Miracle-dz/UniFAR-FTUs huggingface dataset:
Electron-hole diffusion lengths > 175 μm in solution-grown CH3NH3PbI3 single crystals

Balanced carrier diffusion in perovskites The efficient operation of solar cells based on inorganic-organic perovskites requires balanced transport of positive and negative charge carriers over long distances. Dong et al. used a top-seeded solution growth method to obtain millimeter-scale single crystals of the organolead trihalide perovskite CH3NH3PbI3. Under low light illumination, the electron and hole diffusion lengths exceeded 3 mm, and under full sunlight illumination, they exceeded 175 µm. Science, this issue p. 967 Large perovskite crystals can exhibit long and balanced carrier diffusion lengths. Long, balanced electron and hole diffusion lengths greater than 100 nanometers in the polycrystalline organolead trihalide compound CH3NH3PbI3 are critical for highly efficient perovskite solar cells. We found that the diffusion lengths in CH3NH3PbI3 single crystals grown by a solution-growth method can exceed 175 micrometers under 1 sun (100 mW cm−2) illumination and exceed 3 millimeters under weak light for both electrons and holes. The internal quantum efficiencies approach 100% in 3-millimeter-thick single-crystal perovskite solar cells under weak light. These long diffusion lengths result from greater carrier mobility, longer lifetime, and much smaller trap densities in the single crystals than in polycrystalline thin films. The long carrier diffusion lengths enabled the use of CH3NH3PbI3 in radiation sensing and energy harvesting through the gammavoltaic effect, with an efficiency of 3.9% measured with an intense cesium-137 source.
Molecular Evolutionary Pathways toward Two Successful Community-Associated but Multidrug-Resistant ST59 Methicillin-Resistant Staphylococcus aureus Lineages in Taiwan: Dynamic Modes of Mobile Genetic Element Salvages

Clonal complex 59 (CC59) Staphylococcus aureus in Taiwan includes both methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). As the most prominent community-associated MRSA (CA-MRSA) in Taiwan, CC59 has two major clones characterized as PVL-negative SCCmec IV (carrying the staphylococcal cassette chromosome mec IV but Panton-Valentine leukocidin-negative) and PVL-positive SCCmec V (5C2&5). We investigated the drug resistance, phylogeny and the distribution and sequence variation of SCCmec, staphylococcal bacteriophage φSA3, genomic island νSaβ and MES (an enterococcal mobile genetic element conferring multidrug resistance) in 195 CC59 S. aureus. Sequencing and PCR mapping revealed that all of the CC59/SCCmec V (5C2&5) MRSA strains had acquired MESPM1 or its segregants, and obtained a φSA3-related fragment in νSaβ. In contrast, MES6272-2 and MES4578, which showed gentamicin resistance that was not encoded by MESPM1, were dominant in SCCmec IVg MRSA. Translocation of a whole φSA3 into νSaβ instead of only a φSA3-related fragment was common in SCCmec IVg MRSA. However, the non-subtype-g SCCmec IV MRSA (SCCmec IVa is the major) still carried MES and νSaβ structures similar to those in SCCmec V (5C2&5) MRSA. A minimum spanning tree constructed by multiple-locus variable-number tandem repeat analysis revealed that SCCmec IVg MRSA and SCCmec V (5C2&5) MRSA grouped respectively in two major clades. The CC59 MSSA was equally distributed among the two clades, while the non-subtype-g SCCmec IV MRSA mostly clustered with SCCmec V (5C2&5) MRSA. Our findings strongly suggest that CC59 MSSA acquired divergent mobile genetic elements and evolved to SCCmec IVg MRSA and SCCmec V (5C2&5) MRSA/non-subtype-g SCCmec IV MRSA independently. The evolutionary history of CC59 S. aureus explains how mobile genetic elements increase the antimicrobial resistance and virulence and contribute to the success of CA-MRSA in Taiwan.
Transcriptional regulation of the chicken hydroxyindole‐O‐methyltransferase gene by the cone–rod homeobox‐containing protein

Functional differentiation of photoreceptor cells involves the expression of two sets of genes: those encoding the proteins of the phototransduction cascade and those encoding the enzymes of melatonin synthesis. The transcription factor Crx (cone−rod homeobox) plays a major role in the differentiation and maintenance of the photoreceptor phenotype. Previous studies have shown that this effect of Crx is correlated with its ability to transactivate several genes of the phototransduction cascade. Here, we show that Crx can also act on the gene encoding the melatonin‐synthesizing enzyme, hydroxyindole‐O‐methyltransferase (HIOMT, EC 2.1.1.4). Three of the six putative Crx‐binding sites found in the chicken HIOMT promoter interact directly with recombinant Crx and bound a pineal/retina‐specific protein showing DNA‐binding characteristics similar to those of Crx. In transient transfection experiments, Crx transactivated transcription of a reporter gene from the chicken HIOMT promoter. Transactivation was observed even with a portion of promoter carrying only one Crx‐binding site and it was abolished by a mutation in this cis‐regulatory element. These data indicate that Crx may participate in photoreceptor cell differentiation, not only by acting on the genes of the phototransduction cascade, but also by controlling the expression of the genes involved in melatonin synthesis.
Genetic alterations in human gastrointestinal cancers. The application to molecular diagnosis

Gastrointestinal cancers involve genetic alterations in multiple oncogenes, multiple tumor suppressor genes, and multiple DNA repair genes. However, common and different genetic changes are observed in esophageal, gastric, and colorectal carcinomas, respectively. Inactivation of the p53 gene and expression of CD44 abnormal transcripts are common events that serve as a powerful tool for cancer diagnosis. Gene amplification of cyclin D is found preferentially in esophageal cancer, whereas gene amplification of cyclin E and c‐met is frequently associated with gastric cancer. Mutations of the cyclin‐dependent kinase inhibitor genes also occur in esophageal and gastric cancers. However, the scenario of multiple gene changes differs depending on the two histologic types of gastric cancer, because they may have different genetic pathways. Interestingly, the frequency of genetic instability is also quite different between the two types of gastric cancer.
The 5′-untranslated region of the mouse mammary tumor virus mRNA exhibits cap-independent translation initiation

In this study, we demonstrate the identification of an internal ribosome entry site (IRES) within the 5′-untranslated region (5′-UTR) of the mouse mammary tumor virus (MMTV). The 5′-UTR of the full-length mRNA derived from the infectious, complete MMTV genome was cloned into a dual luciferase reporter construct containing an upstream Renilla luciferase gene (RLuc) and a downstream firefly luciferase gene (FLuc). In rabbit reticulocyte lysate, the MMTV 5′-UTR was capable of driving translation of the second cistron. In vitro translational activity from the MMTV 5′-UTR was resistant to the addition of m7GpppG cap-analog and cleavage of eIF4G by foot-and-mouth disease virus (FMDV) L-protease. IRES activity was also demonstrated in the Xenopus laevis oocyte by micro-injection of capped and polyadenylated bicistronic RNAs harboring the MMTV-5′-UTR. Finally, transfection assays showed that the MMTV-IRES exhibits cell type-dependent translational activity, suggesting a requirement for as yet unidentified cellular factors for its optimal function.
A transportation problem based resource allocation scheme for an LTE-Advanced system with carrier aggregation

We formulate the downlink cross-carrier resource allocation problem in LTE-Advanced carrier aggregation (CA) systems as an unbalanced transportation problem (TP). The component carriers (CC's), or their associated sub-bands, represent the supply points whereas the users requesting traffic represent the demand points. The cost of shipping from a supply point to a demand point is set as a function of the proportional fairness (PF) metric. To balance the problem, an artificial supply point is added to represent the shortage in satisfying the demand in case of an overloaded system. Backward compatibility with LTE Release 8 (Rel-8) is guaranteed by adjusting the cost of shipping to Rel-8 users to restrict them to operate on a single CC. The results show that using Vogel approximation method (VAM), which is a sub-optimal efficient method, provides near-optimum results in terms of throughput and fairness and is much faster than the simplex method for optimal solution. The proposed TP-based scheduler is shown to achieve considerable performance improvement over a PF scheduler that handles multiple CC's in the presence of both Rel-8 and LTE-A users.
Fluconazole Loading Dose Pharmacokinetics and Safety in Infants

Background: Invasive candidiasis is a leading cause of morbidity and mortality in critically ill infants. Prompt administration of fluconazole and achievement of the therapeutic target (area under the curve 0 to 24 hours >400 mg*h/L) improve outcomes in candidemic patients. A loading dose of fluconazole is advised for older patients but has not been evaluated in infants. We sought to determine the pharmacokinetics and safety of a fluconazole loading dose in infants at risk for invasive fungal infection. Methods: We enrolled 10 hospitalized infants <60 days old with suspected systemic fungal infection in this open-label study; 9 received a 25-mg/kg fluconazole loading dose followed by a maintenance dose of 12 mg/kg every 24 hours for 4 additional days. Plasma samples were obtained following the loading and steady-state doses (doses 3–5). We used a 1-compartment model to fit the data to estimate pharmacokinetic indices. Results: Data from 57 drug concentrations obtained from 8 infants (median postnatal age, 16 days [interquartile range, 13–32] and median gestational age, 37 weeks [35–38]) showed that the median fluconazole area under the curve 0 to 24 hours (mg*h/L) in this population was 479 (347–496). Of the 8 infants who received the loading dose, 5 (63%) achieved the therapeutic target on the first day of dosing, and all infants achieved a fluconazole 24-hour trough concentration >8 &mgr;g/mL. No adverse events were thought to be related to fluconazole therapy. Conclusions: A loading dose of fluconazole (25 mg/kg) was safe in this small cohort of young infants and achieved the therapeutic target more rapidly than traditional dosing.
Sonographic Differentiation of Asymptomatic Diffuse Thyroid Disease from Normal Thyroid: A Prospective Study

BACKGROUND AND PURPOSE: There is no useful guide or study related to the differentiation of asymptomatic diffuse thyroid disease from normal thyroid by using thyroid US. This study was prospectively designed to evaluate the efficacy of the use of real-time thyroid sonography as performed by an experienced radiologist for the identification of asymptomatic DTD. MATERIALS AND METHODS: From January 2008 to December 2008, 2267 patients underwent thyroid sonography in our hospital by 1 radiologist. Each patient's thyroid was prospectively classified as being in 1 of 4 of the following diagnostic categories on the basis of the sonographic features as determined with the use of real-time sonography: suggestive for DTD, suspicious for DTD, indeterminate, and no evidence of DTD. We calculated the diagnostic efficacy of the sonographic classifications compared with the pathology results. RESULTS: Sonographic classifications for DTD in 340 patients who underwent thyroid surgery because of thyroid malignancy or other causes included the following: suggestive for DTD (n = 32), suspicious for DTD (n = 39), indeterminate (n = 18), and no evidence of DTD (n = 251). On the pathology, HT (n = 33), chronic lymphocytic thyroiditis (n = 27), diffuse hyperplasia (n = 2), and NTP (n = 278) were identified. There were true-positive cases (n = 50), true-negative cases (n = 244), false-positive cases (n = 21), and false-negative cases (n = 7). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for a diagnosis of asymptomatic DTD were 87.7%, 92.1%, 70.4%, 97.2%and 91.3%, respectively. CONCLUSIONS: The present sonographic classification based on real-time sonography of the thyroid is a useful tool for differentiating asymptomatic DTD from normal thyroid.
Clinical and histopathological appraisal of preoperative irradiation for adenocarcinoma of the pancreatoduodenal region

A retrospective review to elucidate the rationale of preoperative irradiation was made on 18 carcinomas of the head of the pancreas area. After 50 Gy/25 fractions of 10 MV X‐ray was given, all 18 tumors decreased from 3.3±0.8 cm to 2.0±0.7 cm. At the surgical operation, 16 patients (89%) received pancreatic resection, without operative death. Histologically, in 13 of these 16 cases, the population of severely degenerative cancer cells (SDCC) was more than 1/3 of all cancer cells, and SDCCs were likely to locate at the periphery (advancing point of carcinoma). These histological patterns were considered as favorable to improve the operative curability. However, in the remaining three cases, the SDCC population was less than 1/3. In addition, nonaffected (i.e., viable) cancer cells were detected at the periphery, and this is an extremely adverse condition for subsequent surgery. Chronic pancreatitis in the noncancerous area was present in these three cases, but not in the 13 cases. Therefore, the most significant factor that predicts the radioresistance especially at the periphery of the carcinoma was considered to be coexisting chronic pancreatitis.
Sensor-Driven Area Coverage for an Autonomous Fixed-Wing Unmanned Aerial Vehicle

Area coverage with an onboard sensor is an important task for an unmanned aerial vehicle (UAV) with many applications. Autonomous fixed-wing UAVs are more appropriate for larger scale area surveying since they can cover ground more quickly. However, their non-holonomic dynamics and susceptibility to disturbances make sensor coverage a challenging task. Most previous approaches to area coverage planning are offline and assume that the UAV can follow the planned trajectory exactly. In this paper, this restriction is removed as the aircraft maintains a coverage map based on its actual pose trajectory and makes control decisions based on that map. The aircraft is able to plan paths in situ based on sensor data and an accurate model of the on-board camera used for coverage. An information theoretic approach is used that selects desired headings that maximize the expected information gain over the coverage map. In addition, the branch entropy concept previously developed for autonomous underwater vehicles is extended to UAVs and ensures that the vehicle is able to achieve its global coverage mission. The coverage map over the workspace uses the projective camera model and compares the expected area of the target on the ground and the actual area covered on the ground by each pixel in the image. The camera is mounted on a two-axis gimbal and can either be stabilized or optimized for maximal coverage. Hardware-in-the-loop simulation results and real hardware implementation on a fixed-wing UAV show the effectiveness of the approach. By including the already developed automatic takeoff and landing capabilities, we now have a fully automated and robust platform for performing aerial imagery surveys.
Mutual Information based Sensor Registration and Calibration

Knowledge of calibration, that defines the location of sensors relative to each other, and registration, that relates sensor response due to the same physical phenomena, are essential in order to be able to fuse information from multiple sensors. In this paper, a mutual information (MI) based approach for automatic sensor registration and calibration is presented. Unsupervised learning of a nonparametric sensing model by maximizing mutual information between signal streams is used to relate information from different sensors, allowing unknown sensor registration and calibration to be determined. Experiments conducted in an office environment are used to illustrate the effectiveness of the proposed technique. Two laser sensors are used to capture people mobbing in an arbitrarily manner in the environment and MI from a number of attributes of the motion are used for relating the signal streams from the sensors. Thus the sensor registration and calibration is achieved without using artificial patterns or pre-specified motions
Quickest Detection POMDPs With Social Learning: Interaction of Local and Global Decision Makers

We consider how local and global decision policies interact in stopping time problems such as quickest time change detection. Individual agents make myopic local decisions via social learning, that is, each agent records a private observation of a noisy underlying state process, selfishly optimizes its local utility and then broadcasts its local decision. Given these local decisions, how can a global decision maker achieve quickest time change detection when the underlying state changes according to a phase-type distribution? This paper presents four results. First, using Blackwell dominance of measures, it is shown that the optimal cost incurred in social-learning-based quickest detection is always larger than that of classical quickest detection. Second, it is shown that in general the optimal decision policy for social-learning-based quickest detection is characterized by multiple thresholds within the space of Bayesian distributions. Third, using lattice programming and stochastic dominance, sufficient conditions are given for the optimal decision policy to consist of a single linear hyperplane, or, more generally, a threshold curve. Estimation of the optimal linear approximation to this threshold curve is formulated as a simulation-based stochastic optimization problem. Finally, this paper shows that in multiagent sensor management with quickest detection, where each agent views the world according to its prior, the optimal policy has a similar structure to social learning.
A System Dynamics Innovation Diffusion Model Applied to Carbon Nanotube Manufacturing

The transition of advanced technologies from their nascent state to viable commercial entities is a critical step in assuring the United States’ national technological superiority and support is often required to incubate such technological developments. We propose a spiral development to investigate the possible scenarios, underlying economics, and risk associated with scaling up carbon nanotube (CNT) production to a commercially viable scale. As the first stage of this proposed effort, this study characterizes the potential scenarios by which a CNT manufacturing company can generate positive annual net revenue and potentially be considered a competitive manufacturer of CNT products on an industrial scale. Subsequent stages of this effort will determine the potential risks associated with investing in the current CNT research and production efforts from a macro perspective. This study investigates the necessary adoption fractions, contact rates, production capacities, production costs, product prices, monetary support, and time necessary for the company of interest to be considered a commercially viable producer of CNT products. The subsidization required to generate varied profit margins is also explored. The application of system dynamics models determined to approximately represent the real diffusion and production of both CNT sheet and CNT yarn products generates insight regarding policy improvement for the company to achieve competitive commercial CNT production and provide an assessment of when CNT production may be profitable. This study should not be used as the basis for decision making due to the fact that the analysis is based on notional data and scenarios.
NAEVOCYTE TRIGGERING BY RECOMBINANT HUMAN GROWTH HORMONE

The influence of growth hormone and insulin‐like growth factor I on human melanocytes is being increasingly recognized. Clinical evidence has shown that when recombinant human growth hormone (hGH) is administered to children of short stature, the growth of melanocytic naevi is boosted. This study was conducted on 56 hGH‐triggered naevi and nine similar lesions excised before or after hGH therapy for hypopituitarism and Turner's syndrome. A series of 40 naevi excised from age‐matched healthy children served as controls. Atypicality of naevocytes was investigated using image analysis, AgNOR counts, immunohistochemistry (HMB‐45, NKI‐C3, Ki‐67, anti‐bcl‐2‐oncoprotein), and DNA flow cytometry. The data associate hGH treatment with anisokaryosis and increased AgNOR and Ki‐67 counts in naevocytes. The same cells also show abnormal patterns of HMB‐45 immunolabelling. These indications of naevocyte activation were not suggestive of malignant transformation. hGH‐triggered melanocytomas should be added to the list of atypical melanocytic naevi. The long‐term evolution of these lesions remains unknown and the potential risk of malignant transformation awaits careful evaluation.
Routine HIV-1 genotyping as a tool to identify dual infections

Objectives:The incidence of HIV-1 dual infections is generally thought to be low, but as dual infections have been associated with accelerated disease progression, its recognition is clinically important. Methods to identify HIV-1 dual infections are time consuming and are not routinely performed. Design:Genotyping of the HIV-1 protease and reverse transcriptase (prot/RT) genes is commonly performed in the western world to detect drug-resistance mutations in clinical isolates. In our hospital, prot/RT baseline sequencing is part of the patient care for all newly infected patients in the Amsterdam region since 2003. We reasoned that degenerate base codes in this sequence could indicate either extensive viral evolution or infection with multiple HIV-1 strains. Methods:We amplified, cloned and sequenced multiple HIV-1 envelope (env)-V3 and gag sequences from patients with 34 or more (range 34–99) degenerate base codes in the ViroSeq genotyping RT sequence (37 out of 1661 available records) to estimate the number of HIV-1 dual infections in this group. Results:Of the 37 patients included in this study, 16 (43.2%, equal to 1% of the 1661 total records) had an HIV-1 dual infection based on phylogenetic analysis of env-V3/gag sequences. If only sequences with 45 or more degenerate base codes were taken into account, 73.3% of patients showed evidence of a dual infection. Conclusion:We describe an additional use of routinely performed HIV-1 genotyping. In patients with a high number of degenerate bases (≥ 34) in RT it is important to consider the possibility of a dual HIV-1 infection.
The Relationship Between Body Fat Percentage and Body Mass Index in Overweight and Obese Individuals in an Urban African Setting

The increase in the prevalence of overweight and obesity in both developed and developing countries is associated with musculoskeletal and other non-communicable diseases. To address this, an accurate measure of body adiposity, bearing in mind several shortcomings of body mass index (BMI), should be used. This study determined the relationship between BMI and body fat (BF)% among adult Nigerians of different ethnic groups residing in an urban setting. Using multistage cluster sampling technique were recruited 1571 subjects (>18 years; male=51.2%) in a cross-sectional study. Body adiposity indices were assessed using BMI and BF%. Using BF%, the result shows that a total number of 156 (9.9%) had low BF% while 291 (18.5%) had very high BF%, while the BMI classifications of body adiposity, 68 (4.3%) were underweight while 271 (17.3%) were obese. There was a strong and positive statistical relationship between BF% and BMI when both were paired without controlling for gender and age (r=0.81, P<0.01). The results show that there is a strong positive association between BMI and BF%, and age and sex are predictors of this association.