Miracle-dz/UniFAR-FTUs huggingface dataset:
DNA from lake sediments reveals long-term ecosystem changes after a biological invasion

Rabbits have had a stronger impact on the landscape and plant communities of a remote island than one century of climate change. What are the long-term consequences of invasive species? After invasion, how long do ecosystems require to reach a new equilibrium? Answering these questions requires long-term, high-resolution data that are vanishingly rare. We combined the analysis of environmental DNA extracted from a lake sediment core, coprophilous fungi, and sedimentological analyses to reconstruct 600 years of ecosystem dynamics on a sub-Antarctic island and to identify the impact of invasive rabbits. Plant communities remained stable from AD 1400 until the 1940s, when the DNA of invasive rabbits was detected in sediments. Rabbit detection corresponded to abrupt changes of plant communities, with a continuous decline of a dominant plant species. Furthermore, erosion rate abruptly increased with rabbit abundance. Rabbit impacts were very fast and were stronger than the effects of climate change during the 20th century. Lake sediments can allow an integrated temporal analysis of ecosystems, revealing the impact of invasive species over time and improving our understanding of underlying mechanisms.
Central nervous system shutdown underlies acute cold tolerance in tropical and temperate Drosophila species

ABSTRACT When cooled, insects first lose their ability to perform coordinated movements (CTmin) after which they enter chill coma (chill coma onset, CCO). Both these behaviours are popular measures of cold tolerance that correlate remarkably well with species distribution. To identify and understand the neuromuscular impairment that causes CTmin and CCO we used inter- and intraspecific model systems of Drosophila species that have varying cold tolerance as a consequence of adaptation or cold acclimation. Our results demonstrate that CTmin and CCO correlate strongly with a spreading depolarization (SD) within the central nervous system (CNS). We show that this SD is associated with a rapid increase in extracellular [K+] within the CNS causing neuronal depolarization that silences the CNS. The CNS shutdown is likely to be caused by a mismatch between passive and active ion transport within the CNS and in a different set of experiments we examine inter- and intraspecific differences in sensitivity to SD events during anoxic exposure. These experiments show that cold adapted or acclimated flies are better able to maintain ionoregulatory balance when active transport is compromised within the CNS. Combined, we demonstrate that a key mechanism underlying chill coma entry of Drosophila is CNS shutdown, and the ability to prevent this CNS shutdown is therefore an important component of acute cold tolerance, thermal adaptation and cold acclimation in insects. Highlighted Article: Cold exposure causes Drosophila to enter a comatose state as a result of the loss of central nervous system function. This is associated with a spreading depolarization and the ability to prevent this underlies acute cold tolerance.
The upregulation of TMPRSS4, partly ascribed to the downregulation of miR-125a-5p, promotes the growth of human lung adenocarcinoma via the NF-κB signaling pathway

In this study, with the aid of microarray technology, transmembrane protease serine 4 (TMPRSS4), a novel member of the serine protease family, was found to be upregulated in the majority of lung adenocarcinoma (LUAD) tissues compared to normal lung tissues. Of note, the clinical significance of TMPRSS4 in LUAD has not yet been reported, at least to the best of our knowledge. Through immunohistochemistry assays, we found that TMPRSS4 was overexpressed in LUAD tissues and that the TMPRSS4 expression level was also proportionally associated with the AJCC clinical stage, T stage and pathological grade. Moreover, a high expression of TMPRSS4 was found to be associated with adverse outcomes and was a significant independent factors predicting a poor prognosis. To elucidate the possible mechanisms responsible for the overexpression of TMPRSS4, we examined at microRNAs (miRNAs or miRs), which are small non-coding RNAs commonly dysregulated in human malignancies and are known to promote carcinogenesis by interacting with other types of RNAs. By means of bioinformatics analysis, a miRNA potentially targeting TMPRSS4 mRNA, namely miR-125a-5p, was selected. Dual luciferase reporter gene assays were then performed to verify the interaction. The results of MTT assays and apoptotic assays revealed that miR-125a-5p significantly inhibited cell growth and enhanced apoptosis, and the silencing of TMPRSS4 had similar effects. Furthermore, we observed that either the overexpression of miR-125a-5p or the silencing of TMPRSS4 prevented the activation of the nuclear factor (NF)-κB signaling pathway. On the whole, our findings illustrate that TMPRSS4 may be a candidate oncogene and may thus serve as a prognostic biomarker for LUAD, and its overexpression may be partly ascribed to the downregulation of miR-125a-5p. The dysregulation of miR-125a-5p and TMPRSS4 affect the biological function of LUAD cells via the NF-κB signaling pathway. The miR-125a-5p/TMPRSS4/NF-κB axis may thus provide novel insight into the pathogenic mechanisms of LUAD and may be used in the development of novel treatment strategies for LUAD.
Dishevelled-2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis

Dishevelled (Dvl)-2 represents one of the cytoplasmic proteins, which serves as a pivotal hub in signaling intermediates through a number of different signaling pathways associated with the Wnt family. The aim of the present study was to investigate the roles and mechanisms of Dvl-2 on synovial fibroblasts (SFBs) in osteoarthritis (OA). A Cell Counting kit-8 (CCK-8) assay was used to determine cell viability. An alkaline phosphatase (ALP) test kit was used to measure the activity of ALP. Western blot and reverse transcription-quantitative polymerase chain reaction analysis were used to evaluate the protein and mRNA expression, respectively. The results suggest that depletion of Dvl-2 significantly decreased the expression of osteoprotegerin (OPG) and ALP (P<0.05) and significantly increased the expression of receptor activator of nuclear factor-κB ligand (RANKL), ALP, osteonectin (ON), osteocalcin (OCN) and osterix (P<0.05). In addition, the depletion of Dvl-2 also significantly inhibited the expression of runt-related transcription factor 2 (Runx-2) and β-catenin in SFBs (P<0.05). The effect of Dvl-2 over-expression was opposite to the effect of Dvl-2 silencing. The inactivation of Wnt3a reversed the effect of Dvl-2 silencing. In conclusion, the results indicate that Dvl-2 regulated osteogenic differentiation of SFBs in OA.
Evidence for policy and practice.

Review Group (with institutions) Advisory group (with institutions) Conflicts of interest (if any) Neither Sultan Barakat nor Kathryn Rzeszut has any personal, financial or professional interests which would influence the conduct or outcomes of this systematic review. However, Sultan Barakat has previously published on the topic of multidonor trust funds (see ‘The Failed Promise of Multi-Donor Trust Funds’, Policy Studies, 2009) and thus may be perceived as having a bias towards confirming previous findings.
Blood-Brain Barrier Disruption and Perivascular Beta-Amyloid Accumulation in the Brain of Aged Rats with Spontaneous Hypertension: Evaluation with Dynamic Contrast-Enhanced Magnetic Resonance Imaging

Objective Whether blood-brain barrier (BBB) disruption induced by chronic spontaneous hypertension is associated with beta-amyloid (Aβ) accumulation in the brain remains poorly understood. The purpose of this study was to investigate the relationship between BBB disruption and Aβ influx and accumulation in the brain of aged rats with chronic spontaneous hypertension. Materials and Methods Five aged spontaneously hypertensive rats (SHRs) and five age-matched normotensive Wistar-Kyoto (WKY) rats were studied. The volume transfer constant (Ktrans) obtained from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was used to evaluate BBB permeability in the hippocampus and cortex in vivo. The BBB tight junctions, immunoglobulin G (IgG), Aβ, and amyloid precursor protein (APP) in the hippocampus and cortex were examined with immunohistochemistry. Results As compared with WKY rats, the Ktrans values in the hippocampus and cortex of the SHRs increased remarkably (0.316 ± 0.027 min−1 vs. 0.084 ± 0.017 min−1, p < 0.001 for hippocampus; 0.302 ± 0.072 min−1 vs. 0.052 ± 0.047 min−1, p < 0.001 for cortex). Dramatic occludin and zonula occludens-1 losses were detected in the hippocampus and cortex of SHRs, and obvious IgG exudation was found there. Dramatic Aβ accumulation was found and limited to the area surrounding the BBB, without extension to other parenchyma regions in the hippocampus and cortex of aged SHRs. Alternatively, differences in APP expression in the hippocampus and cortex were not significant. Conclusion Blood-brain barrier disruption is associated with Aβ influx and accumulation in the brain of aged rats with chronic spontaneous hypertension. DCE-MRI can be used as an effective method to investigated BBB damage.
Relationship between Anxiety and Cognitive Function and Activity of Daily Living in Ischemic Stroke Patients

Objective:To determine the relationship between anxiety and cognitive function and activity of daily living(ADL)in eschemic stroke patients.Methods:All the patients enrolled were divided into the anxiety group and non-anxiety group according to their Hamilton rating scale for anxiety(HAMA)scores.The cognitive functions were evaluated with Mini Mental State Examination(MMSE)during the acute seizure phase.Barthel index was used to evaluate the ADL.The relationship between HAMA score and MMSE,Barthel index score was analyzed.Results:The scores of MMSE,and Barthel index were obviously lower in the anxiety group(P0.05,0.01). Soma-anxiety,mental anxiety and the overall HAMA score were closely related to the MMSE score and factor score of attention/calculation,short-term memory and language comprehension(P0.05,0.01). The relationship between soma-anxiety,mental anxiety and the overall HAMA score and Barthel index score was significant(P0.05,0.01). Conclusion:Anxiety condition is correlated to the cognitive function and ADL in the acute seizure phase of ischemic stroke.Anxiety is negatively influence the cognitive function and ADL of ischemic stroke patients.
Reopening Openness to Experience: A Network Analysis of Four Openness to Experience Inventories

ABSTRACT Openness to Experience is a complex trait, the taxonomic structure of which has been widely debated. Previous research has provided greater clarity of its lower order structure by synthesizing facets across several scales related to Openness to Experience. In this study, we take a finer grained approach by investigating the item-level relations of four Openness to Experience inventories (Big Five Aspects Scale, HEXACO–100, NEO PI–3, and Woo et al.'s Openness to Experience Inventory), using a network science approach, which allowed items to form an emergent taxonomy of facets and aspects. Our results (N = 802) identified 10 distinct facets (variety-seeking, aesthetic appreciation, intellectual curiosity, diversity, openness to emotions, fantasy, imaginative, self-assessed intelligence, intellectual interests, and nontraditionalism) that largely replicate previous findings as well as three higher order aspects: two that are commonly found in the literature (intellect and experiencing; i.e., openness), and one novel aspect (open-mindedness). In addition, we demonstrate that each Openness to Experience inventory offers a unique conceptualization of the trait, and that some inventories provide broader coverage of the network space than others. Our findings establish a broader consensus of Openness to Experience at the aspect and facet level, which has important implications for researchers and the Openness to Experience inventories they use.
Light absorption and partitioning in Arctic Ocean surface waters: impact of multiyear ice melting

Ice melting in the Arctic Ocean exposes the surface water to more radiative energy with poorly understood effects on photo-biogeochemical processes and heat deposi-tion in the upper ocean. In August 2009, we documented the vertical variability of light absorbing components at 37 stations located in the southeastern Beaufort Sea including both Mackenzie River-influenced waters and polar mixed layer waters. We found that melting multiyear ice released significant amount of non-algal particulates (NAP) near the sea surface relative to subsurface waters. NAP absorption coefficients at 440 nm (a NAP (440)) immediately below the sea surface were on average 3-fold (up to 10-fold) higher compared to subsurface values measured at 2–3 m depth. The impact of this unusual feature on the light transmission and remote sensing reflectance (R rs) was further examined using a ra-diative transfer model. A 10-fold particle enrichment homogeneously distributed in the first meter of the water column slightly reduced photosynthetically available and usable radiation (PAR and PUR) by ∼ 6 and ∼ 8 %, respectively, relative to a fully homogenous water column with low particle concentration. In terms of R rs , the particle enrichment significantly flattered the spectrum by reducing the R rs by up to 20 % in the blue-green spectral region (400–550 nm). These results highlight the impact of meltwater on the concentration of particles at sea surface, and the need for considering non-uniform vertical distribution of particles in such systems when interpreting remotely sensed ocean color. Spectral slope of a NAP spectra calculated in the UV (ultraviolet) domain decreased with depth suggesting that this parameter is sensitive to detritus composition and/or diagenesis state (e.g., POM (particulate organic matter) photobleaching).
Placental stem cells pre-treated with a hyaluronan mixed ester of butyric and retinoic acid to cure infarcted pig hearts: a multimodal study.

AIMS Pre-treating placenta-derived human mesenchymal stem cells (FMhMSCs) with a hyaluronan mixed ester of butyric and retinoic acid (HBR) potentiates their reparative capacity in rodent hearts. Our aim was to test FMhMSCs in a large-animal model by employing a novel combination of in vivo and ex vivo analyses. METHODS AND RESULTS Matched regional quantifications of myocardial function and viability were performed by magnetic resonance imaging (MRI) and positron emission tomography (PET) 4 weeks after myocardial infarction combined with intramyocardial injection of FMhMSCs (n = 7), or HBR-pre-treated FMhMSCs (HBR-FMhMSCs, n = 6), or saline solution (PBS, n = 7). Sham-operated pigs (n = 4) were used as control animals. Despite no differences in the ejection fraction and haemodynamics, regional MRI revealed, in pigs treated with HBR-FMhMSCs compared with the other infarcted groups, a 40% smaller infarct scar size and a significant improvement of the end-systolic wall thickening and circumferential shortening of the infarct border zone. Consistently, PET showed that myocardial perfusion and glucose uptake were, respectively, 35 and 23% higher in the border zone of pigs treated with HBR-FMhMSCs compared with the other infarcted groups. Histology supported in vivo imaging; the delivery of HBR-FMhMSCs significantly enhanced capillary density and decreased fibrous tissue by approximately 68%. Moreover, proteomic analysis of the border zone in the HBR-FMhMSCs group and the FMhMSCs group indicated, respectively, 45 and 30% phenotypic homology with healthy tissue, while this homology was only 26% in the border zone of the PBS group. CONCLUSION Our results support a more pronounced reparative potential of HBR-pre-treated FMhMSCs in a clinically relevant animal model of infarction and highlight the necessity of using combined diagnostic imaging to avoid underestimations of stem cell therapeutic effects in the heart.
Measurement of Posterior Corneal Astigmatism by the IOLMaster 700.

PURPOSE To provide the first description of posterior corneal astigmatism as measured by the IOLMaster 700 (Carl Zeiss Meditec, Jena, Germany) and assess how its characteristics compare to previous measurements from other devices. METHODS A total of 1,098 routine IOLMaster 700 biometric measurements were analyzed to provide magnitudes and orientation of steep and flat axes of anterior and posterior corneal astigmatism. Subgroup analysis was conducted to assess correlation of posterior corneal astigmatism characteristics to anterior corneal astigmatism and describe the distribution of posterior corneal astigmatism with age. RESULTS Mean posterior corneal astigmatism was 0.24 ± 0.15 diopters (D). The steep axis of posterior corneal astigmatism was vertically oriented in 73.32% of measurements. Correlation between the magnitude of anterior and posterior corneal astigmatism was greatest when the steep axis of the anterior corneal astigmatism was oriented vertically (r = 0.68, P < .0001). Vertical orientation of the steep axis of anterior corneal astigmatism became less common as age increased, whereas for posterior corneal astigmatism it remained by far the most common orientation. CONCLUSIONS This first description of posterior corneal astigmatism measurement by the IOLMaster 700 found the average magnitude of posterior corneal astigmatism and proportion of vertical orientation of steep axis was lower than previous estimates. The IOLMaster 700 appears capable of providing enhanced biometric measurement for individualized surgical planning. [J Refract Surg. 2018;34(5):331-336. ].
Uptake of Second Dose of Measles-Containing Vaccine among Children in Kakamega County , Kenya

Measles is a major cause of death and complications among young children worldwide despite the availability of a safe and effective vaccine. Per annum over 158,000 cases of measles mortality are reported globally, especially in Africa and Asia. In Kenya, 59 per a million measles incidence were reported in 2011. Approximately 80.1 % of the children aged less than 5 years receive a first dose of measles-containing vaccine in Kakamega County. In 2013 a second dose of measles-containing vaccine was introduced in the routine immunization system. A cross-sectional survey was conducted to determine the coverage of second dose of measles-containing vaccine among children in Kakamega County. Thirty clusters were selected using probability proportional to size with replacement, 19 households were surveyed per cluster and data of the youngest child aged between 24-35 months collected. Among the 571 children surveyed; the coverage of second dose of measles-containing vaccine was 102 (17.9%) (95%CI = 14.9% to 21.3%). The caretaker’s awareness of the second dose of measles-containing vaccine, time taken to the nearest health facility, uptake of Pentavalent 3 and uptake of at least two doses of Vitamin A was significantly associated with the uptake of the second dose of measles-containing vaccine, p-value of 0.0000, 0.0010, 0.0281 and 0.0000respectively. The second dose of measles-containing vaccine coverage in Kakamega is very low, strategies focusing on demand creation, conducting outreach services in hard-toreach areas and ensuring that there are no missed opportunities should be put in place to increase utilization of the second dose of measles-containing vaccine across the County.
Endogenous pain modulation in chronic orofacial pain: a systematic review and meta-analysis

Abstract Abnormal endogenous pain modulation was suggested as a potential mechanism for chronic pain, ie, increased pain facilitation and/or impaired pain inhibition underlying symptoms manifestation. Endogenous pain modulation function can be tested using psychophysical methods such as temporal summation of pain (TSP) and conditioned pain modulation (CPM), which assess pain facilitation and inhibition, respectively. Several studies have investigated endogenous pain modulation function in patients with nonparoxysmal orofacial pain (OFP) and reported mixed results. This study aimed to provide, through a qualitative and quantitative synthesis of the available literature, overall estimates for TSP/CPM responses in patients with OFP relative to controls. MEDLINE, Embase, and the Cochrane databases were searched, and references were screened independently by 2 raters. Twenty-six studies were included for qualitative review, and 22 studies were included for meta-analysis. Traditional meta-analysis and robust variance estimation were used to synthesize overall estimates for standardized mean difference. The overall standardized estimate for TSP was 0.30 (95% confidence interval: 0.11-0.49; P = 0.002), with moderate between-study heterogeneity (Q [df = 17] = 41.8, P = 0.001; I2 = 70.2%). Conditioned pain modulation's estimated overall effect size was large but above the significance threshold (estimate = 1.36; 95% confidence interval: −0.09 to 2.81; P = 0.066), with very large heterogeneity (Q [df = 8] = 108.3, P < 0.001; I2 = 98.0%). Sensitivity analyses did not affect the overall estimate for TSP; for CPM, the overall estimate became significant if specific random-effect models were used or if the most influential study was removed. Publication bias was not present for TSP studies, whereas it substantially influenced CPM's overall estimate. These results suggest increased pain facilitation and trend for pain inhibition impairment in patients with nonparoxysmal OFP.
Apoptotic vesicles: deathly players in cancer‐associated coagulation

Although cancer is associated with coagulation disorders, it is still unclear how the combination of tumor cell and host factors enhance the hypercoagulable state of cancer patients. Emerging evidence points to a central role for tumor endosomal and plasma membrane‐derived vesicular components in the pathogenesis of cancer‐related thrombosis. In particular, tumor cell membranes and extracellular vesicles (EV) harbor lipids and proteinaceous coagulation factors able to initiate multiple points within the coagulation matrix. The impact of chemotherapy upon a host already burdened with a hypercoagulable state increases the risk of pathological coagulation. We argue that chemotherapy‐induced EV harbor the most active components for cancer related thrombosis and discuss how membrane components of the host and tumor act to initiate coagulation to enhance thrombotic risk in cancer patients.