Miracle-dz/UniFAR-FTUs huggingface dataset:
The Transcription Coactivator Cbp Is a Dynamic Component of the Promyelocytic Leukemia Nuclear Body

The transcription coactivator and histone acetyltransferase CAMP response element–binding protein (CBP) has been demonstrated to accumulate in promyelocytic leukemia (PML) bodies. We show that this accumulation is cell type specific. In cells where CBP does not normally accumulate in PML bodies, it can be induced to accumulate in PML bodies through overexpression of either CBP or Pml, but not Sp100. Using fluorescence recovery after photobleaching, we demonstrate that CBP moves rapidly into and out of PML bodies. In contrast, Pml and Sp100 are relatively immobile in the nucleoplasm and within PML nuclear bodies. They possess the characteristics expected of proteins that would play a structural role in the integrity of these subnuclear domains. Our results are consistent with CBP being a dynamic component of PML bodies and that the steady-state level in these structures can be modulated by Pml.
The in vivo Levels of Matrix Metalloproteinase-1 and -8 in Gingival Crevicular Fluid during Initial Orthodontic Tooth Movement

Orthodontic force induces biochemical responses in the periodontal ligament (PDL), but the matrix metalloproteinase (MMP)-dependent molecular mechanisms in orthodontically induced periodontal remodeling have remained unclear. Previous studies indicate that mechanical stress induces MMP-1 production in human PDL cells in vitro. We tested the hypothesis whether the in vivo levels, molecular forms, and degree of activation of MMP-1 and MMP-8 in gingival crevicular fluid (GCF) reflect an early stage of orthodontic tooth movement. Molecular forms of MMP-1 and MMP-8 were analyzed by Western blot, and MMP-8 levels by quantitative immunofluoro-metric assay (IFMA). The results showed that GCF MMP-8 levels for orthodontically treated teeth were significantly higher at 4-8 hrs after force application than before activation, and when compared with the control teeth (p < 0.05). Analysis of our data indicates that the cells within the periodontium are up-regulated to produce MMP-8, and the increased expression and activation of GCF MMP-8 reflect enhanced periodontal remodeling induced by orthodontic force.
Exosomes reflect the hypoxic status of glioma cells and mediate hypoxia-dependent activation of vascular cells during tumor development

Hypoxia, or low oxygen tension, is a major regulator of tumor development and aggressiveness. However, how cancer cells adapt to hypoxia and communicate with their surrounding microenvironment during tumor development remain important questions. Here, we show that secreted vesicles with exosome characteristics mediate hypoxia-dependent intercellular signaling of the highly malignant brain tumor glioblastoma multiforme (GBM). In vitro hypoxia experiments with glioma cells and studies with patient materials reveal the enrichment in exosomes of hypoxia-regulated mRNAs and proteins (e.g., matrix metalloproteinases, IL-8, PDGFs, caveolin 1, and lysyl oxidase), several of which were associated with poor glioma patient prognosis. We show that exosomes derived from GBM cells grown at hypoxic compared with normoxic conditions are potent inducers of angiogenesis ex vivo and in vitro through phenotypic modulation of endothelial cells. Interestingly, endothelial cells were programmed by GBM cell-derived hypoxic exosomes to secrete several potent growth factors and cytokines and to stimulate pericyte PI3K/AKT signaling activation and migration. Moreover, exosomes derived from hypoxic compared with normoxic conditions showed increased autocrine, promigratory activation of GBM cells. These findings were correlated with significantly enhanced induction by hypoxic compared with normoxic exosomes of tumor vascularization, pericyte vessel coverage, GBM cell proliferation, as well as decreased tumor hypoxia in a mouse xenograft model. We conclude that the proteome and mRNA profiles of exosome vesicles closely reflect the oxygenation status of donor glioma cells and patient tumors, and that the exosomal pathway constitutes a potentially targetable driver of hypoxia-dependent intercellular signaling during tumor development.
Differentiating Science Instruction: Secondary science teachers' practices

This descriptive study investigated the implementation practices of secondary science teachers who differentiate instruction. Participants included seven high school science teachers purposefully selected from four different schools located in a mid-Atlantic state. Purposeful selection ensured participants included differentiated instruction (DI) in their lesson implementation. Data included semi-structured interviews and field notes from a minimum of four classroom observations, selected to capture the variety of differentiation strategies employed. These data were analyzed using a constant-comparative approach. Each classroom observation was scored using the validated Differentiated Instruction Implementation Matrix-Modified, which captured both the extent to which critical indicators of DI were present in teachers' instruction and the performance levels at which they engaged in these components of DI. Results indicated participants implemented a variety of differentiation strategies in their classrooms with varying proficiency. Evidence suggested all participants used instructional modifications that required little advance preparation to accommodate differences in students' interests and learning profile. Four of the seven participants implemented more complex instructional strategies that required substantial advance preparation by the teacher. Most significantly, this study provides practical strategies for in-service science teachers beginning to differentiate instruction and recommendations for professional development and preservice science teacher education.
Epoxyeicosatrienoic acids and the soluble epoxide hydrolase are determinants of pulmonary artery pressure and the acute hypoxic pulmonary vasoconstrictor response

Recent findings have indicated a role for cytochrome P‐450 (CYP) epoxygenase‐derived epoxyeicosatrienoic acids (EETs) in acute hypoxic pulmonary vasoconstriction (HPV). Given that the intracellular concentration of EETs is determined by the soluble epoxide hydrolase (sEH), we assessed the influence of the sEH and 11,12‐EET on pulmonary artery pressure and HPV in the isolated mouse lung. In lungs from wild‐type mice, HPV was significantly increased by sEH inhibition, an effect abolished by pretreatment with CYP epoxygenase inhibitors and the EET antagonist 14,15‐EEZE. HPV and EET production were greater in lungs from sEH‐/‐ mice than from wild‐type mice and sEH inhibition had no further effect on HPV, while MSPPOH and 14,15‐EEZE decreased the response. 11,12‐EET increased pulmonary artery pressure in a concentration‐dependent manner and enhanced HPV via a Rho‐dependent mechanism. Both 11,12‐EET and hypoxia elicited the membrane translocation of a transient receptor potential (TRP) C6‐V5 fusion protein, the latter effect was sensitive to 14,15‐ EEZE. Moreover, while acute hypoxia and 11,12‐EET increased pulmonary pressure in lungs from TRPC6+/‐ mice, lungs from TRPC6‐/‐ mice did not respond to either stimuli. These data demonstrate that CYP‐derived EETs are involved in HPV and that EET‐induced pulmonary contraction under normoxic and hypoxic conditions involves a TRPC6‐dependent pathway. — Keserü, B. , Barbosa‐Sicard, E. , Popp, R. , Fisslthaler, B. , Dietrich, A. , Gudermann, T. , Hammock, B. D. , Falck, J. R. , Weissmann, N. , Busse, R. , Fleming, I. Epoxyeicosatrienoic acids and the soluble epoxide hydrolase are determinants of pulmonary artery pressure and the acute hypoxic pulmonary vasoconstrictor response. FASEB J. 22, 4306–4315 (2008)
Implementing curriculum-embedded formative assessment in primary school science classrooms

The implementation of formative assessment strategies is challenging for teachers. We evaluated teachers’ implementation fidelity of a curriculum-embedded formative assessment programme for primary school science education, investigating both material-supported, direct application and subsequent transfer. Furthermore, the relationship between implementation fidelity and teacher variables was explored. N = 17 German primary school teachers participated in professional development on formative assessment, N = 11 teachers formed a control group. Teachers’ implementation fidelity was evaluated via classroom observations student ratings and an analysis of students’ workbooks, focusing on the frequency and quality of intended formative assessment elements (assessments, feedback and instructional adaptations). Regarding direct application, treatment group teachers’ implementation fidelity was high, with slight variations in quality. Regarding transfer, implementation fidelity was lower but teachers still implemented more formative assessment elements than the control group. Teachers’ pedagogical content knowledge and their evaluation of the formative assessment intervention were associated with implementation success.
None-Native University Students’ Perception of Plagiarism

Plagiarism is a complex issue especially among non-native students and it has received a lot of attention from researchers and scholars of academic writing. Some scholars attribute this problem to cultural perceptions and different attitudes toward texts. This study evaluates student perception of different aspects of plagiarism. A small group of postgraduate students in a Malaysian university were asked to categorize ten cases of plagiarism instances. They were also asked to identify plagiarized paraphrased versions of five excerpts of different source texts provided. The findings showed that students had misconceptions about different aspect of plagiarism including citation conventions, collusion, using another writer’s idea, and style of writing. Further analysis has shown that students were aware that plagiarism is wrong but they could not correctly identify the multiple forms in which plagiarism could happen. These findings indicate that students need to be taught and exposed to various forms and layers of plagiarism so that they would know how best to avoid it in their own writing.
Radiofrequency Catheter Ablation in Children with Supraventricular Tachycardias: Intermediate Term Follow Up Results

The Purpose of the Study Radiofrequency (RF) catheter ablation represents an important advance in the management of children with cardiac arrhythmias and has rapidly become the standard and effective line of therapy for supraventricular tachycardias (SVTs) in pediatrics. The purpose of this study was to evaluate the intermediate term follow up results of radiofrequency catheter ablation in treatment of SVT in pediatric age group. Methods A total of 60 pediatric patients (mean age = 12.4 ± 5.3 years, ranged from 3 years to 18 years; male: female = 37:23; mean body weight was 32.02 ± 12.3 kg, ranged from 14 kg to 60 kg) with clinically documented SVT underwent an electrophysiologic study (EPS) and RF catheter ablation at Children's Hospital Mansoura University, Mansoura, Egypt during the period from January 2008 to December 2009 and they were followed up until October 2011. Results The arrhythmias included atrioventricular reentrant tachycardia (AVRT; n = 45, 75%), atrioventricular nodal reentrant tachycardia (AVNRT; n = 6, 10%), and atrial tachycardia (AT; n = 9, 15%). The success rate of the RF catheter ablation was 93.3% for AVRT, 66.7% for AVNRT, and 77.8% for AT, respectively. Procedure-related complications were infrequent (7/60, 11.7%), (atrial flutter during RF catheter ablation (4/60, 6.6%); ventricular fibrillation during RF catheter ablation (1/60, 1.6%); transient complete heart block during RF catheter ablation (2/60, 3.3%)). The recurrence rate was 8.3% (5/60) during a follow-up period of 34 ± 12 months. Conclusion RF catheter ablation is an effective and safe method to manage children with SVT.
Effects of Forage Sources on Rumen Fermentation Characteristics, Performance, and Microbial Protein Synthesis in Midlactation Cows

Eight multiparous Holstein cows (632±12 kg BW; 135±16 DIM) were used in a replicated 4×4 Latin square design to evaluate the effects of forage sources on rumen fermentation characteristics, performance, and microbial protein (MCP) synthesis. The forage portion of the diets contained alfalfa hay (AH), oat hay (OH), Leymus chinensis (LC), or rice straw (RS) as the primary source of fiber. Diets were isonitrogenous and isocaloric, and cows were fed four corn silages based total mixed rations with equivalent nonfiber carbohydrate (NFC) and forage neutral detergent fiber (NDF). Dry matter intake was not affected by the source of dietary forages, ranging from 18.83 to 19.20 kg/d, consequently, milk yield was similar among diets. Because of the numerical differences in milk fat and milk protein concentrations, 4% FCM and ECM yields were unchanged (p>0.05). Mean rumen pH, NH3-N content, and concentrations of volatile fatty acids in the rumen fluid were not affected by the treatments (p>0.05). Dietary treatments did not affect the total tract apparent digestibility of dry matter, organic matter, and crude protein (p>0.05); however, digestibility of NDF and acid detergent fiber in RS diet was higher compared with AH, OH, and LC diets (p<0.05). Total purine derivative excretion was higher in cows fed AH, OH, and LC diets compared with those fed RS diet (p<0.05), consequently, estimated MCP synthesis was 124.35 g/d higher in cows fed AH diet compared with those fed RS diet (p<0.05). The results indicated that cows fed AH, OH, LC, and RS diets with an equivalent forage NDF and NFC have no unfavourable effect on the ruminal fermentation and productive parameters.
Psephological investigations: Tweets, votes, and unknown unknowns in the republican nomination process

This paper analyzes the utility of using information contained within Twitter posts in predicting electoral outcomes. Particularly, we are interested in patterns in Twitter communications that can help explain differences between published opinion polls and the actual vote. We consider three categories of models. The first is a mentions model that examines the correspondence between the prevalence of communications about a candidate and electoral outcomes. The second series of models treat Twitter similar to a prediction market, aggregating not candidate preferences but predictions of the electoral result. Last, we consider whether the rediffusion of tweets about a candidate is a reliable predictor of the candidate's performance. The results find inconsistent support for the predictive value of Twitter mentions as an estimate of the overall vote, but these communications provide some evidence of otherwise undetected shifts in momentum with respect to the aggregated predictions of candidate performance and message rediffusion via retweets containing information about a particular candidate. Given the nature of the information extractable, these data are most sensitive to detecting changes in momentum.
Development, validation and implementation of a quantitative food frequency questionnaire to assess habitual vitamin D intake.

BACKGROUND A well-designed, validated quantitative food frequency questionnaire (FFQ) could offer an efficient and cost-effective method for assessing habitual vitamin D intake. The present study aimed to describe the development, validation and implementation of a vitamin D FFQ. METHODS National food consumption survey data obtained from Irish adults (18-64 years) were used to identify foods that contribute 95% of vitamin D intake. A winter-based validation study was carried out for the resulting FFQ in 120 females, including 98 women [mean (SD) 65.0 (7.3) years] and 22 girls [12.2 (0.8) years], using a 14-day diet history (DH) as a comparator. Serum 25(OH)D concentrations were analysed. Validity coefficients were calculated using the method of triads. Cross-classification and Bland-Altman analysis were also performed. RESULTS Median (interquartile range) vitamin D intakes (including the contribution from nutritional supplements) were 5.4 (3.7) and 3.7 (5.9) μg day(-1) from the FFQ and DH, respectively and intakes of vitamin D from food sources were 3.6 (3.1) and 2.4 (2.2) μg day(-1) . The FFQ and DH classified 86% and 87% of individuals into the same and adjacent thirds of wintertime serum 25(OH)D status, respectively. There was a strong association (r = 0.71, P < 0.0001) and no significant systematic or proportional bias observed for the difference between estimates from the FFQ and DH. The validity coefficient for the FFQ was 0.92 (95% confidence interval = 0.80-0.97). Repeatability analysis (n = 56) performed 6-12 months later showed no significant difference in estimates of vitamin D between administrations. CONCLUSIONS The data obtained in the present study indicate high validity and good reproducibility of a short, interviewer-administered FFQ for vitamin D.
The double-peaked 2008 outburst of the accreting milli-second X-ray pulsar, IGR J00291+5934

Context. In August 2008, the accreting milli-second X-ray pulsar (AMXP), IGR J00291+5934, underwent an outburst lasting ∼100 days, the first since its discovery in 2004. Aims. We present data from the 2008 double-peaked outburst of IGR J00291+5934 from Faulkes Telescope North, the Isaac Newton Telescope, the Keck Telescope, PAIRITEL, the Westerbork Synthesis Radio Telescope and the Swift, XMM-Newton and RXTE X-ray missions. We study the outburst’s evolution at various wavelengths, allowing us to probe accretion physics in this AMXP. Methods. We study the light curve morphology, presenting the first radio-X-ray spectral energy Distributions (SEDs) for this source and the most detailed UV-IR SEDs for any outbursting AMXP. We show simple models that attempt to identify the emission mechanisms responsible for the SEDs. We analyse short-timescale optical variability, and compare a medium resolution optical spectrum with those from 2004. Results. The outburst morphology is unusual for an AMXP, comprising two peaks, the second containing a “plateau” of ∼10 days at maximum brightness within 30 days of the initial activity. This has implications on duty cycles of short-period X-ray transients. The X-ray spectrum can be fitted by a single, hard power-law. We detect optical variability of ∼0.05 mag, on timescales of minutes, but find no periodic modulation. In the optical, the SEDs contain a blue component, indicative of an irradiated disc, and a transient near-infrared (NIR) excess. This excess is consistent with a simple model of an optically thick synchrotron jet (as seen in other outbursting AMXPs), however we discuss other potential origins. The optical spectrum shows a double-peaked Hα profile, a diagnostic of an accretion disc, but we do not clearly see other lines (e.g. He I, II) that were reported in 2004. Conclusions. Optical/IR observations of AMXPs appear to be excellent for studying the evolution of both the outer accretion disc and the inner jet, and may eventually provide us with tight constraints to model disc-jet coupling in accreting neutron stars.
Toluene biofiltration by the fungus Scedosporium apiospermum TB1.

The performance of biofilters inoculated with the fungus Scedosporium apiospermum was evaluated. This fungus was isolated from a biofilter which operated with toluene for more than 6 months. The experiments were performed in a 2.9 L reactor packed with vermiculite or with vermiculite-granular activated carbon as packing material. The initial moisture content of the support and the inlet concentration of toluene were 70% and 6 g/m3, respectively. As the pressure drop increased from 5-40 mm H2O a strong initial growth was observed. Stable operation was maintained for 20 days with a moisture content of 55% and a biomass of 33 mg biomass/g dry support. These conditions were achieved with intermittent addition of culture medium, which permitted a stable elimination capacity (EC) of 100 g/m3(reactor)h without clogging. Pressure drop across the bed and CO2 production were related to toluene elimination. Measurement of toluene, at different levels of the biofilter, showed that the system attained higher local EC (200 g/m3(r)h) at the reactor outlet. These conditions were related to local humidity conditions. When the mineral medium was added periodically before the EC decreases, EC of approximately 258 g/m3(r)h were maintained with removal efficiencies of 98%. Under these conditions the average moisture content was 60% and 41 mg biomass/g dry support was produced. No sporulation was observed. Evaluation of bacterial content and activities showed that the toluene elimination was only due to S. apiospermum catabolism.
Lectures on string/brane cosmology

An overview of some cosmological aspects of string theory is presented. Recent developments are emphasized, especially the attempts to derive inflation or alternatives to inflation from the dynamics of branes in string theory. Time-dependent backgrounds with potential cosmological implications, such as those provided by negative tension branes and S-branes and the rolling string tachyon are also discussed.
Student absenteeism: whose responsibility?

Internationally there is concern about levels of student absenteeism. Research underpinning this article consisted of a survey of academic staff and 25 interviews with first year students in a well regarded ‘new’ university in Britain. The article explores the issue of poor attendance and why a significant number of students seem to have difficulty in engaging fully with their studies in order to become critical and autonomous learners. We look at the role of government policy, institutions, educators, students, schools and parents.
Open Evaluation: A Vision for Entirely Transparent Post-Publication Peer Review and Rating for Science

The two major functions of a scientific publishing system are to provide access to and evaluation of scientific papers. While open access (OA) is becoming a reality, open evaluation (OE), the other side of the coin, has received less attention. Evaluation steers the attention of the scientific community and thus the very course of science. It also influences the use of scientific findings in public policy. The current system of scientific publishing provides only journal prestige as an indication of the quality of new papers and relies on a non-transparent and noisy pre-publication peer-review process, which delays publication by many months on average. Here I propose an OE system, in which papers are evaluated post-publication in an ongoing fashion by means of open peer review and rating. Through signed ratings and reviews, scientists steer the attention of their field and build their reputation. Reviewers are motivated to be objective, because low-quality or self-serving signed evaluations will negatively impact their reputation. A core feature of this proposal is a division of powers between the accumulation of evaluative evidence and the analysis of this evidence by paper evaluation functions (PEFs). PEFs can be freely defined by individuals or groups (e.g., scientific societies) and provide a plurality of perspectives on the scientific literature. Simple PEFs will use averages of ratings, weighting reviewers (e.g., by H-index), and rating scales (e.g., by relevance to a decision process) in different ways. Complex PEFs will use advanced statistical techniques to infer the quality of a paper. Papers with initially promising ratings will be more deeply evaluated. The continual refinement of PEFs in response to attempts by individuals to influence evaluations in their own favor will make the system ungameable. OA and OE together have the power to revolutionize scientific publishing and usher in a new culture of transparency, constructive criticism, and collaboration.