Miracle-dz/UniFAR-FTUs huggingface dataset:
Aerobic Capacity Reference Data in 3816 Healthy Men and Women 20–90 Years

Purpose To provide a large reference material on aerobic fitness and exercise physiology data in a healthy population of Norwegian men and women aged 20–90 years. Methods Maximal and sub maximal levels of VO2, heart rate, oxygen pulse, and rating of perceived exertion (Borg scale: 6–20) were measured in 1929 men and 1881 women during treadmill running. Results The highest VO2max and maximal heart rate among men and women were observed in the youngest age group (20–29 years) and was 54.4±8.4 mL·kg−1·min−1 and 43.0±7.7 mL·kg−1·min−1 (sex differences, p<0.001) and 196±10 beats·min−1 and 194±9 beats·min−1 (sex differences, p<0.05), respectively, with a subsequent reduction of approximately 3.5 mL·kg−1·min−1 and 6 beats·min−1 per decade. The highest oxygen pulses were observed in the 3 youngest age groups (20–29 years, 30–39 years, 40–49 years) among men and women; 22.3 mL·beat−1±3.6 and 14.7 mL·beat−1±2.7 (sex differences, p<0.001), respectively, with no significant difference between these age groups. After the age of 50 we observed an 8% reduction per decade among both sexes. Borg scores appear to give a good estimate of the relative exercise intensity, although observing a slightly different relationship than reported in previous reference material from small populations. Conclusion This is the largest European reference material of objectively measured parameters of aerobic fitness and exercise-physiology in healthy men and women aged 20–90 years, forming the basis for an easily accessible, valid and understandable tool for improved training prescription in healthy men and women.
Elevation of serum creatine kinase in response to medical treatment of Graves’ disease in children

We describe two cases of increases in serum creatine kinase (CK) concentrations in children undergoing treatment of Graves’ disease with antithyroid medications. Presenting complaints consisted of myalgias and muscle cramping in both patients, and increases in serum CK levels were noted 1 mo after initiation of antithyroid drugs. Both patients were euthyroid at the time of CK elevation. While the mechanisms for this process are not clear, it is likely that the acute decrease of thyroid hormones in tissues following a state of chronic hyperthyroidism may result in relative hypothyroid states and subsequent alterations in CK concentrations.
Relationship between ambulatory BP and clinical outcomes in patients with hypertensive CKD.

BACKGROUND AND OBJECTIVES Abnormal ambulatory BP (ABP) profiles are commonplace in CKD, yet the prognostic value of ABP for renal and cardiovascular outcomes is uncertain. This study assessed the relationship of baseline ABP profiles with CKD progression and subsequent cardiovascular outcomes to determine the prognostic value of ABP beyond that of clinic BP measurements. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS Between 2002 and 2003, 617 African Americans with hypertensive CKD treated to a clinic BP goal of <130/80 mmHg were enrolled in this prospective, observational study. Participants were followed for a median of 5 years. Primary renal outcome was a composite of doubling of serum creatinine, ESRD, or death. The primary cardiovascular outcome was a composite of myocardial infarction, hospitalized congestive heart failure, stroke, revascularization procedures, cardiovascular death, and ESRD. RESULTS Multivariable Cox proportional hazard analysis showed that higher 24-hour systolic BP (SBP), daytime, night-time, and clinic SBP were each associated with subsequent renal (hazard ratio, 1.17-1.28; P<0.001) and cardiovascular outcomes (hazard ratio, 1.22-1.32; P<0.001). After controlling for clinic SBP, ABP measures were predictive of renal outcomes in participants with clinic SBP <130 mmHg (P<0.05 for interaction). ABP predicted cardiovascular outcomes with no interaction based on clinic BP control. CONCLUSIONS ABP provides additional information beyond that of multiple clinic BP measures in predicting renal and cardiovascular outcomes in African Americans with hypertensive CKD. The primary utility of ABP in these CKD patients was to identify high-risk individuals among those patients with controlled clinic BP.
Analysis of ku80-Mutant Mice and Cells with Deficient Levels of p53

ABSTRACT Absence of Ku80 results in increased sensitivity to ionizing radiation, defective lymphocyte development, early onset of an age-related phenotype, and premature replicative senescence. Here we investigate the role of p53 on the phenotype of ku80-mutant mice and cells. Reducing levels of p53 increased the cancer incidence for ku80−/− mice. About 20% ofku80 −/− p53 +/− mice developed a broad spectrum of cancer by 40 weeks and allku80 −/− p53 −/− mice developed pro-B-cell lymphoma by 16 weeks. Reducing levels of p53 rescued populations of ku80 −/− cells from replicative senescence by enabling spontaneous immortalization. The double-mutant cells are impaired for the G1/S checkpoint due to the p53 mutation and are hypersensitive to γ-radiation and reactive oxygen species due to the Ku80mutation. These data show that replicative senescence is caused by a p53-dependent cell cycle response to damaged DNA inku80 −/− cells and that p53 is essential for preventing very early onset of pro-B-cell lymphoma inku80 −/− mice.
BMR in a Brazilian adult probability sample: the Nutrition, Physical Activity and Health Survey

Abstract Objective To measure BMR in a probability sample of adults from an urban city of Brazil and to compare indirectly measured BMR (BMRi) with BMR predicted from different equations. Design BMR data were obtained by indirect calorimetry and estimated by different predictive equations (Schofield; Harris and Benedict; Henry and Rees). Anthropometric and body composition measures were also obtained. Setting The Nutrition, Physical Activity and Health Survey (PNAFS), a household survey conducted in Niterói, Rio de Janeiro state, Brazil. Subjects Representative sample of 529 adults (aged ≥20 years; 339 females) living in Niterói, Rio de Janeiro state, Brazil. Results Mean BMRi values were 5839·7 (se 73·9) kJ/d and 4758·1 (se 39·5) kJ/d for men and women, respectively. Predicted BMR by all equations was significantly higher (difference between means and 95 % CI did not include zero) than BMRi in both men and women of all ages. Overall bias in BMR (predicted BMR minus BMRi) using the Schofield equations (overestimation of about 20 %) was higher than when using the Henry and Rees equations (13 % and 16 % overestimation for males and females, respectively). The percentage of individuals whose BMR predicted by the Schofield equations fell within 10 % of BMRi was very low (7·8 % and 14·1 % of males and females, respectively). Conclusions Current available predictive equations of BMR are not adequate to estimate BMR in Brazilians living in Niterói, Rio de Janeiro, Brazil.
Nitric oxide and cutaneous active vasodilation during heat stress in humans.

Whether nitric oxide (NO) is involved in cutaneous active vasodilation during hyperthermia in humans is unclear. We tested for a role of NO in this process during heat stress (water-perfused suits) in seven healthy subjects. Two forearm sites were instrumented with intradermal microdialysis probes. One site was perfused with the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) dissolved in Ringer solution to abolish NO production. The other site was perfused with Ringer solution only. At those sites, skin blood flow (laser-Doppler flowmetry) and sweat rate were simultaneously and continuously monitored. Cutaneous vascular conductance, calculated from laser-Doppler flowmetry and mean arterial pressure, was normalized to maximal levels as achieved by perfusion with the NO donor nitroprusside through the microdialysis probes. Under normothermic conditions, L-NAME did not significantly reduce cutaneous vascular conductance. During hyperthermia, with skin temperature held at 38-38.5 degreesC, internal temperature rose from 36.66 +/- 0.10 to 37.34 +/- 0.06 degreesC (P < 0.01). Cutaneous vascular conductance at untreated sites increased from 12 +/- 2 to 44 +/- 5% of maximum, but only rose from 13 +/- 2 to 30 +/- 5% of maximum at L-NAME-treated sites (P < 0.05 between sites) during heat stress. L-NAME had no effect on sweat rate (P > 0.05). Thus cutaneous active vasodilation requires functional NO synthase to achieve full expression.
Gene expression defines natural changes in mammalian lifespan

Mammals differ more than 100‐fold in maximum lifespan, which can be altered in either direction during evolution, but the molecular basis for natural changes in longevity is not understood. Divergent evolution of mammals also led to extensive changes in gene expression within and between lineages. To understand the relationship between lifespan and variation in gene expression, we carried out RNA‐seq‐based gene expression analyses of liver, kidney, and brain of 33 diverse species of mammals. Our analysis uncovered parallel evolution of gene expression and lifespan, as well as the associated life‐history traits, and identified the processes and pathways involved. These findings provide direct insights into how nature reversibly adjusts lifespan and other traits during adaptive radiation of lineages.
Sulfur Requirements of Container-grown Pin Oak and Japanese Maple

Substrates of container-grown plants are commonly pre-plant amended with sulfated micronutrients to supply micronutrients. However, the cause for the resulting increase in growth may be due to micronutrient addition or to other factors, such as S addition or substrate acidification. Container-grown pin oak (Quercus palustris Münchh) and Japanese maple (Acer palmatum Thunb.) seedlings were grown in a 100% pine bark substrate and amended (or not) with one of the following treatments: control (no amendment), Micromax, K2SO4, H2SO4, HCl, chelated micronutrients, elemental S, or CaSO4. After 11 weeks, dry weights of plants in all treatments supplying S were higher than plants receiving no S. Dry weights of plants in all experiments receiving the chelate treatment were not higher than dry weights for control plants. These data indicated that S, not micronutrient application, was a primary cause of increased growth from the addition of sulfated micronutrients. However, at substrate pH 5.4 dry weight of plants fertilized with Micromax was higher than dry weight of plants fertilized with FeSO4. At a substrate pH of 4.1 there was no difference in dry weight between the Micromax and FeSO4 treatments. Thus, there are conditions such as higher substrate solution pH (5.4 vs. 4.1), where Micromax may prove advantageous over sulfur alone since it would supply micronutrients as well as S.
Assisting the Visually Impaired: Obstacle Detection and Warning System by Acoustic Feedback

The aim of this article is focused on the design of an obstacle detection system for assisting visually impaired people. A dense disparity map is computed from the images of a stereo camera carried by the user. By using the dense disparity map, potential obstacles can be detected in 3D in indoor and outdoor scenarios. A ground plane estimation algorithm based on RANSAC plus filtering techniques allows the robust detection of the ground in every frame. A polar grid representation is proposed to account for the potential obstacles in the scene. The design is completed with acoustic feedback to assist visually impaired users while approaching obstacles. Beep sounds with different frequencies and repetitions inform the user about the presence of obstacles. Audio bone conducting technology is employed to play these sounds without interrupting the visually impaired user from hearing other important sounds from its local environment. A user study participated by four visually impaired volunteers supports the proposed system.
Stress burden and the lifetime incidence of psychiatric disorder in young adults: racial and ethnic contrasts.

BACKGROUND With the exception of studies of individual traumatic events, the significance of stress exposure in psychiatric disorder previously has not been effectively examined. OBJECTIVE To address the hypothesis that accumulated adversity represents an important risk factor for the subsequent onset of depressive and anxiety disorders. DESIGN A community-based study of psychiatric and substance use disorders among a large, ethnically diverse cohort representative of young adults in South Florida. Adversity was estimated with a count of major and potentially traumatic events experienced during one's lifetime and prior to the onset of disorder. SETTING Most interviews took place in the homes of participants, with 30% conducted by telephone. PARTICIPANTS We obtained a random sample of individuals aged 18 to 23 years from a previously studied representative sample of young adolescents. Because participants in the prior study were predominantly boys, a supplementary sample of girls was randomly obtained from the early-adolescence school class rosters. A total of 1803 interviews were completed, representing a success rate of 70.1%. MAIN OUTCOME MEASURES Depressive and anxiety disorders were assessed through computer-assisted personal interviews using the DSM-IV version of the Michigan Composite International Diagnostic Interview. RESULTS Level of lifetime exposure to adversity was found to be associated with an increased risk of subsequent onset of depressive and/or anxiety disorder. This association remained clearly observable when childhood conduct disorder, attention-deficit/hyperactivity disorder, prior substance dependence, and posttraumatic stress disorder were held constant and when the possibility of state dependence effects was considered. CONCLUSION Evidence suggests that high levels of lifetime exposure to adversity are causally implicated in the onset of depressive and anxiety disorders.
Evaluation of clorazepate (Tranxene®) as an anticonvulsant—a pilot study

Desmethyldiazepam—providing the long-term anticonvulsant effect when diazepam is given orally—is conveniently administered as clorazepate (Tranxene®). In this study, clorazepate was compared to phenobarbital as a secondary anticonvulsant in eight ambulatory, adult outpatients. Stable doses of phenytoin were maintained throughout. Drowsiness was present in all on phenobarbital, but there were no clorazepate-related side effects. Seizure control did not differ for each treatment. Addition of common side effects of phenytoin and phenobarbital limited the attained serum levels of each when used together. Clorazepate doses in the 0.56-mg-per-kilogram range gave desmethyldiazepam levels in the 1.0-ug-per-milliliter range. Induction of metabolism was suggested by falling desmethyldiazepam levels despite increasing doses. Clorazepate is an effective, nontoxic secondary anticonvulsant.
Growth hormone (GH)-binding protein in normal and GH-deficient dwarf rats.

There are GH-binding proteins (GHBPs) present in the blood of many species, and these correspond to the extracellular GH-binding domain of the GH receptor. In the rat, GHBP arises by alternative splicing of the GH receptor mRNA, but little is known of the physiological role of circulating GHBP, or its relationship with episodic GH secretion. We have developed a sensitive radioimmunoassay based on recombinant GHBP, and have measured rat GHBP levels in small samples of plasma from normal and GH-deficient dwarf rats. In normal adult rats, GHBP levels were two- to threefold higher in females than in males (16.6 +/- 0.8 vs 6.4 +/- 0.4 micrograms/l, P < 0.001), but this sex difference was not seen in dwarf rats. A continuous infusion of human GH in dwarf males raised plasma GHBP to 23.5 +/- 3.5 micrograms/l compared with 6.7 +/- 0.5 micrograms/l in sham-infused animals, whereas suppression of GH by continuous infusion of a long-acting somatostatin analogue in female dwarf rats had no effect on GHBP. In anaesthetized rats, large changes in plasma GH caused by i.v. administration of rat GH, somatostatin or GH-releasing factor did not affect GHBP acutely. Both GH and GHBP were also measured in serial blood samples from conscious normal and dwarf rats. A sexually dimorphic GH secretory pattern was observed in both strains. Males showed peaks and troughs of GH every 3 h varying over a 100-fold range, whereas females exhibited more continuous GH secretion. Despite the large fluctuations in endogenous GH, GHBP levels remained relatively constant in individual normal or dwarf males, as well as in females of both strains, and there was no significant correlation between GH and GHBP either in individual rats or as a group. Our results suggest that GHBP is GH-dependent in the longer term, and that the higher GHBP levels in female rats require their continuous GH secretory pattern. However, plasma GHBP levels remain stable and are not affected by acute changes in endogenous or exogenous GH.
Semiconducting polymers and quantum dots in luminescent solar concentrators for solar energy harvesting

We compare the performance of luminescent solar concentrators (LSCs) fabricated with polymers and quantum dots to the behavior of laser dye LSCs. Previous research, centered around the use of small molecule laser dyes, was hindered by the lack of materials with small absorption/emission band overlap and longer lifetime. Materials such as semiconducting polymers and quantum dots present qualities that are desirable in LSCs, for example, smaller absorption/emission band overlap, tunable absorption, and longer lifetimes. In this study, the efficiency of LSCs consisting of liquid solutions of semiconducting polymers encased in glass was measured and compared to the efficiency of LSCs based on small molecule dyes and on quantum dots. Factors affecting the optical efficiency of the system such as the luminescing properties of the organic materials were examined. The experimental results were compared to Monte Carlo simulations. Our results suggest that commercially available quantum dots cannot serve as viable .
Inferring the Tsimane's use of color categories from recognition memory

Knowledge of color has strong individual, environmental, and cultural differences that may systematically influence performance in cognitive tasks. For example, color knowledge has been shown to influence recall of color (Persaud & Hemmer, 2014). This manifests as a systematic regression to the mean effect, where memory is biased towards the mean hue of each universal color category. What remains unclear is whether differences, such as culture and environment, might differentially influence memory. We tested recognition memory for color in the Tsimane’ of Bolivia; an indigenous population with little or no modern schooling, whose environment is very different from industrialized societies. We found that recognition regressed towards the mean of some universal color categories, but for others was systematically biased toward neighboring categories. A cluster analysis suggested that the Tsimane’ use five underlying color categories—not the standard universals. This might be shaped by education, language and the environment.
An optimal path to transition in a duct

This paper is concerned with the transition of the laminar flow in a duct of square cross section. As in the similar case of pipe flow, the motion is linearly stable for all Reynolds numbers, rendering this flow a suitable candidate for a study of the ‘bypass’ path to turbulence. It has already been shown that the classical linear optimal perturbation problem, yielding optimal disturbances in the form of longitudinal vortices, fails to provide an ‘optimal’ path to turbulence, i.e. optimal perturbations do not elicit a significant nonlinear response from the flow. Previous simulations have also indicated that a pair of travelling waves generates immediately, by nonlinear quadratic interactions, an unstable mean flow distortion, responsible for rapid breakdown. By the use of functions quantifying the sensitivity of the motion to deviations in the base flow, the optimal travelling wave associated with its specific defect is found by a variational approach. This optimal solution is then integrated in time and shown to display a qualitative similarity to the so-called ‘minimal defect’, for the same parameters. Finally, numerical simulations of an ‘edge state’ are conducted, to identify an unstable solution that mediates laminar–turbulent transition and relate it to results of the optimization procedure.
Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay

Biogenic amines are widely characterized in pathways evaluating reward and punishment, resulting in appropriate aversive or appetitive responses of vertebrates and invertebrates. We utilized the honey bee model and a newly developed spatial avoidance conditioning assay to probe effects of biogenic amines octopamine (OA) and dopamine (DA) on avoidance learning. In this new protocol non-harnessed bees associate a spatial color cue with mild electric shock punishment. After a number of experiences with color and shock the bees no longer enter the compartment associated with punishment. Intrinsic aspects of avoidance conditioning are associated with natural behavior of bees such as punishment (lack of food, explosive pollination mechanisms, danger of predation, heat, etc.) and their association to floral traits or other spatial cues during foraging. The results show that DA reduces the punishment received whereas octopamine OA increases the punishment received. These effects are dose-dependent and specific to the acquisition phase of training. The effects during acquisition are specific as shown in experiments using the antagonists Pimozide and Mianserin for DA and OA receptors, respectively. This study demonstrates the integrative role of biogenic amines in aversive learning in the honey bee as modeled in a novel non-appetitive avoidance learning assay.
Utility of Noninvasive Serum Biomarkers of Liver Fibrosis in Infants with Biliary Atresia

Background: Biliary Atresia (BA) is the most common cause of chronic cholestasis in infants It is a destructive inflammatory obliterative cholangiopathy that affects varying lengths of both intrahepatic and extrahepatic bile ducts. Even after a successful surgery, scarring of the liver can continue, resulting in cirrhosis and its complications. Aim: The aim of this study is to evaluate different serological markers derived from routine investigations in the prediction of liver fibrosis in infants with BA. Methods: This retrospective study included a total of 147 infants with proved diagnosis of BA. We employed six noninvasive scores (FIB-4, FibroQ, King’s score, APRI, GUCI and AAR). Liver fibrosis was classified into 5 grades. For further descriptive purpose, we arbitrarily divided fibrosis grades into early (F1, F2 and F3) and advanced (F4 and F5) fibrosis. Results: FIB-4, FibroQ and King’s score correlated significantly with fibrosis grade (P values were 0.007 and 0.015 respectively) while there was no significant correlation with other studied scores (P value >0.05). FIB-4, FibroQ and King’s score were significantly higher in patients with advanced fibrosis compared to early fibrosis and at cutoff values of 0.0098, 0.0085 and 0.115 respectively they were able to discriminate those with advanced fibrosis with acceptable sensitivity (61.9%-64.3%) and specificity (60.0%-62.9%). Conclusion: FIB-4, FibroQ and King’s score, but not APRI, GUCI and AAR, correlated significantly with fibrosis and could predict those with advanced fibrosis with relatively acceptable performance. These markers may be of help in predicting advanced fibrosis and in long term follow up of infants with BA and reduce the need for repeated liver biopsy.
Learning to Develop Mathematics Lessons with GeoGebra

This paper describes how prospective secondary mathematics teachers’ Technological Pedagogical Content Knowledge (TPCK) and perspectives about teaching and learning mathematics were enriched as they worked individually and in small groups to develop and present lessons with GeoGebra. We also note how Geogebra can be used as a vehicle for building links between a research-focussed university and secondary school teachers.