Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of output spike patterns by phasic inhibition in cerebellar granule cells

The complex interplay of multiple molecular mechanisms taking part to synaptic integration is hard to disentangle experimentally. Therefore, we developed a biologically realistic computational model based on the rich set of data characterizing the cerebellar glomerulus microcircuit. A specific issue was to determine the relative role of phasic and tonic inhibition in dynamically regulating granule cell firing, which has not been clarified yet. The model comprised the excitatory mossy fiber—granule cell and the inhibitory Golgi cell—granule cell synapses and accounted for vesicular release processes, neurotransmitter diffusion and activation of different receptor subtypes. Phasic inhibition was based on stochastic GABA release and spillover causing activation of two major classes of postsynaptic receptors, α1 and α6, while tonic inhibition was based on steady regulation of a Cl− leakage. The glomerular microcircuit model was validated against experimental responses to mossy fiber bursts while metabotropic receptors were blocked. Simulations showed that phasic inhibition controlled the number of spikes during burst transmission but predicted that it specifically controlled time-related parameters (firing initiation and conclusion and first spike precision) when the relative phase of excitation and inhibition was changed. In all conditions, the overall impact of α6 was larger than that of α1 subunit-containing receptors. However, α1 receptors controlled granule cell responses in a narrow ±10 ms band while α6 receptors showed broader ±50 ms tuning. Tonic inhibition biased these effects without changing their nature substantially. These simulations imply that phasic inhibitory mechanisms can dynamically regulate output spike patterns, as well as calcium influx and NMDA currents, at the mossy fiber—granule cell relay of cerebellum without the intervention of tonic inhibition.
Understanding the stigma of mental illness : theory and interventions

Contents List of contributors Foreword Preface 1 The rights of a powerless legion Julio Arboleda-Florez 2 Cross-cultural aspects of the stigma of mental illness Bernice A. Pescosolido, Sigrun Olafsdottir, Jack K. Martin and J. Scott Long The WPA Global Programme against Stigma and Discrimination because of Schizophrenia Norman Sartorius 4 'Fighting stigma and discrimination because of schizophrenia - Open the Doors': a collaborative review of the experience from the German project centres A.E. Baumann, W. Gaebel, et al. 5 Stigma and health care staff Juan J. Lopez-Ibor Jr., Olga Cuenca and Maria-Ines Lopez-Ibor 6 Evaluating programmatic needs concerning the stigma of mental illness Beate Schulze 7 Using the Internet for fighting the stigma of schizophrenia Hugh Schulze 8 Building an evidence base for anti-stigma programming Heather Stuart 9 Other people stigmatize . but, what about us? Attitudes of mental health professionals towards patients with schizophrenia Alp Ucok 10 Implementing anti stigma programmes in Boulder, Colorado and Calgary, Alberta Richard Warner 11 Stigma measurement approaches: conceptual origins and current applications Lawrence H. Yang, Bruce G. Link and Jo C. Phelan Appendix Inventories to measure the scope and impact of stigma experiences from the perspective of those who are stigmatized - consumer and family versions Heather Stuart, Michelle Koller and Roumen Milev Index
Robustness to Faults Promotes Evolvability: Insights from Evolving Digital Circuits

We demonstrate how the need to cope with operational faults enables evolving circuits to find more fit solutions. The analysis of the results obtained in different experimental conditions indicates that, in absence of faults, evolution tends to select circuits that are small and have low phenotypic variability and evolvability. The need to face operation faults, instead, drives evolution toward the selection of larger circuits that are truly robust with respect to genetic variations and that have a greater level of phenotypic variability and evolvability. Overall our results indicate that the need to cope with operation faults leads to the selection of circuits that have a greater probability to generate better circuits as a result of genetic variation with respect to a control condition in which circuits are not subjected to faults.
Retrieval, Monitoring, and Control Processes: A 7 Tesla fMRI Approach to Memory Accuracy

Memory research has been guided by two powerful metaphors: the storehouse (computer) and the correspondence metaphor. The latter emphasizes the dependability of retrieved mnemonic information and draws upon ideas about the state dependency and reconstructive character of episodic memory. We used a new movie to unveil the neural correlates connected with retrieval, monitoring, and control processes, and memory accuracy (MAC), according to the paradigm of Koriat and Goldsmith (1996a,b). During functional magnetic resonance imaging, subjects performed a memory task which required (after an initial learning phase) rating true and false statements [retrieval phase (RP)], making confidence judgments in the respective statement [monitoring phase (MP)], and deciding for either venturing (volunteering) the respective answer or withholding the response [control phase (CP)]. Imaging data pointed to common and unique neural correlates. Activations in brain regions related to RP and MAC were observed in the precuneus, middle temporal gyrus, and left hippocampus. MP was associated with activation in the left anterior and posterior cingulate cortex along with bilateral medial temporal regions. If an answer was volunteered (as opposed to being withheld) during the CP, temporal, and frontal as well as middle and posterior cingulate areas and the precuneus revealed activations. Increased bilateral hippocampal activity was found during withholding compared to volunteering answers. The left caudate activation detected during withholding compared to venturing an answer supports the involvement of the left caudate in inhibiting unwanted responses. Contrary to expectations, we did not evidence prefrontal activations during withholding (as opposed to volunteering) answers. This may reflect our design specifications, but alternative interpretations are put forth.
Addressing Literacy and Numeracy to Improve Diabetes Care

OBJECTIVE Diabetic patients with lower literacy or numeracy skills are at greater risk for poor diabetes outcomes. This study evaluated the impact of providing literacy- and numeracy-sensitive diabetes care within an enhanced diabetes care program on A1C and other diabetes outcomes. RESEARCH DESIGN AND METHODS In two randomized controlled trials, we enrolled 198 adult diabetic patients with most recent A1C ≥7.0%, referred for participation in an enhanced diabetes care program. For 3 months, control patients received care from existing enhanced diabetes care programs, whereas intervention patients received enhanced programs that also addressed literacy and numeracy at each institution. Intervention providers received health communication training and used the interactive Diabetes Literacy and Numeracy Education Toolkit with patients. A1C was measured at 3 and 6 months follow-up. Secondary outcomes included self-efficacy, self-management behaviors, and treatment satisfaction. RESULTS At 3 months, both intervention and control patients had significant improvements in A1C from baseline (intervention −1.50 [95% CI −1.80 to −1.02]; control −0.80 [−1.10 to −0.30]). In adjusted analysis, there was greater improvement in A1C in the intervention group than in the control group (P = 0.03). At 6 months, there were no differences in A1C between intervention and control groups. Self-efficacy improved from baseline for both groups. No significant differences were found for self-management behaviors or satisfaction. CONCLUSIONS A literacy- and numeracy-focused diabetes care program modestly improved self-efficacy and glycemic control compared with standard enhanced diabetes care, but the difference attenuated after conclusion of the intervention.
Extended familism among urban Mexican Americans Anglos and Blacks.

The data for this study were collected in 1974 as part of a larger investigation of a tri-ethnic and geographically isolated urban community in Kansas City Missouri. The research design called for a puposive sample of parents with at least 1 child enrolled in a neighborhood elementary school. Parents were randomly selected from school rosters of parochial elementary schools and from public schools. Complete data were obtained for 143 Anglos 160 Blacks and 152 Mexican Americans. Respondents were interviewed in their homes. The measure of extended familism used in this study has 4 indices: extensity of presence intensity of presence interaction and functionality. Extensity of presence is measured by the total number of households of kin living in the Kansas City area. Intensity of presence is measured by dividing kin into nuclear or extended categories. Intensity with kin is measured by counting the number of kin interacted with at least monthly. Functionality is measured by whether or nor different categories of kin are involved in a variety of mutual aid services and support. The hypothesis that Blacks and Mexican Americans should have higher levels of extended familism than Anglos is largely supported by the data. The data clearly indicate that the Anglos in this sample maintain the lowest level of extended familism on all 4 measures. Mexican Americans appear to have larger families and therefore engage in more extensive kinship. Blacks on the other hand appear to have smaller families and interact with them less but use their kin in a more instrumental fashion as a mutual aid and support system. The pattern that emerges concerning migration is that Anglos migrate away from their kin network whereas Blacks and to an even greater extent Mexican Americans migrate within a kin network. For Blacks and Mexican Americans the functional and/or cultural requirements lead to a much more kinship-involved family pattern.
Evaluation and costs of different haemoglobin methods for use in district hospitals in Malawi

Aims: To evaluate the characteristics of manual haemoglobin methods in use in Malawi and provide evidence for the Ministry of Health in Malawi to enable them to choose a suitable method for district hospitals. Methods: Criteria on accuracy, clinical usefulness, user friendliness, speed, training time, and economic costs were determined by local health professionals and used to compare six different manual haemoglobin methods. These were introduced sequentially into use in a district hospital in Malawi alongside the reference method. Results: HemoCue was the optimal method based on most of the outcome measures but was also the most expensive (US$0.75/test). DHT meter and Jenway colorimeter were the second choice because they were cheaper (US$0.20–0.35/test), but they were not as accurate or user friendly as HemoCue. Conclusions: The process for choosing appropriate laboratory methods is complex and very little guidance is available for health managers in poorer countries. This paper describes the development and testing of a practical model for gathering evidence about test efficiency that could be adapted for use in other resource poor settings.
Bacillus thuringiensis and Bacillus weihenstephanensis Inhibit the Growth of Phytopathogenic Verticillium Species

Verticillium wilt causes severe yield losses in a broad range of economically important crops worldwide. As many soil fumigants have a severe environmental impact, new biocontrol strategies are needed. Members of the genus Bacillus are known as plant growth-promoting bacteria (PGPB) as well as biocontrol agents of pests and diseases. In this study, we isolated 267 Bacillus strains from root-associated soil of field-grown tomato plants. We evaluated the antifungal potential of 20 phenotypically diverse strains according to their antagonistic activity against the two phytopathogenic fungi Verticillium dahliae and Verticillium longisporum. In addition, the 20 strains were sequenced and phylogenetically characterized by multi-locus sequence typing (MLST) resulting in 7 different Bacillus thuringiensis and 13 Bacillus weihenstephanensis strains. All B. thuringiensis isolates inhibited in vitro the tomato pathogen V. dahliae JR2, but had only low efficacy against the tomato-foreign pathogen V. longisporum 43. All B. weihenstephanensis isolates exhibited no fungicidal activity whereas three B. weihenstephanensis isolates showed antagonistic effects on both phytopathogens. These strains had a rhizoid colony morphology, which has not been described for B. weihenstephanensis strains previously. Genome analysis of all isolates revealed putative genes encoding fungicidal substances and resulted in identification of 304 secondary metabolite gene clusters including 101 non-ribosomal polypeptide synthetases and 203 ribosomal-synthesized and post-translationally modified peptides. All genomes encoded genes for the synthesis of the antifungal siderophore bacillibactin. In the genome of one B. thuringiensis strain, a gene cluster for zwittermicin A was detected. Isolates which either exhibited an inhibitory or an interfering effect on the growth of the phytopathogens carried one or two genes encoding putative mycolitic chitinases, which might contribute to antifungal activities. This indicates that chitinases contribute to antifungal activities. The present study identified B. thuringiensis isolates from tomato roots which exhibited in vitro antifungal activity against Verticillium species.
Representations of men's violence against women : audio-visual texts and their reception

Portrayals of sexual and/or domestic violence committed by men against women appear in the television schedules and in movie theatres on almost a daily basis. There is a long established tradition of concern about how depictions of violence in the audiovisual media can impact on audiences. However, minimal consideration has been given to what kind of discursive 'messages' such portrayals might contain and how audiences engage with these. This research explores to what extent audio-visual portrayals of violence against women might offer certain ways of reading and understanding that violence, and how women audiences interpret these. It investigates this through both textual and reception analyses, with the framework through which reception is examined being directly related to the textual material itself. The study assesses to what degree audio-visual texts are capable of structuring audience interpretation, and whether there is any direct relationship between this and how women viewers actually read the texts. Four audio-visual products are examined: the Hollywood film The Accused; an episode of the soap opera EastEnders; the television play Closing Ranks; and an edition of Crimewatch UK. Ninety one women, formed into 14 focus groups, participated in the reception research. Half of these women had experienced violence of a domestic and/or sexual nature, whilst the other half had no experience of such violence. Groups further varied in terms of nationality (Scottish and English), class (working-class and middle-class) and ethnic background (white, Asian and Afro-Caribbean). The research demonstrates that neither audio-visual texts nor their reception can be considered outwith an appreciation of how social and cultural factors influence both. The media are involved in the circulation of cultural meanings about acts of violence against women and in mediating this cannot help but draw on existing discourses which surround such violence. As audiences, women also draw on their socialised conceptions of such violence, though how they engage with and read its representation is affected by their social and cultural positionings and their own lived experiences.
Minor iridoids from Scutellaria albida ssp. albida. Inhibitory potencies on lipoxygenase, linoleic acid lipid peroxidation and antioxidant activity of iridoids from Scutellaria sp.

A new iridoid glycoside, 6′-O-E-caffeoyl-mussaenosidic acid1, in addition to one known aglycon, four known triterpenes and one known flavonoid, were isolated from the aerial parts of Scutellaria albida subsp. albida. Furthermore, 12 iridoids with similar structures isolated from Scutellaria sp. , were examined for their inhibitory potency on lipoxygenase and lipid peroxidation, as well as their antioxidant activity, in comparison to known antioxidants e.g. caffeic acid, nordihydroguaretic acid (NDGA) and trolox. AAPH, DPPH and soybean lipoxygenase (LOX) assays were used for the tests. This investigation led to interesting observations considering the Structure-Activity Relationship. According to our results, the presence of a p-coumaroyl group optimized and even dramatically changed the biological responses of the investigated iridoids.
A statistical resolution theory of the AR method of spectral analysis

The autoregressive (AR) method of spectral analysis is widely used in diverse areas for its solid theoretical foundation, interesting physical interpretation, computational efficiency, and, more importantly, high resolution capability. Various aspects of its statistical performance have been investigated. However, the resolution probability that provides the most rigorous description of the spectrum resolution capability is still not available in the literature. In this paper, by formulating the resolution event in the framework of statistical decision theory and directly determining its probability from its characteristic function, we obtain an exact asymptotic formula for the probability of resolution. On this basis, we determine the limiting resolving behavior of the sample AR spectrum and develop the corresponding geometrical insight in the parametric space. Simulation and numerical results are also presented to confirm and illustrate the theory.
Cell cortex composition and homeostasis resolved by integrating proteomics and quantitative imaging

The cellular actin cortex is the cytoskeletal structure primarily responsible for the control of animal cell shape and as such plays a central role in cell division, migration, and tissue morphogenesis. Due to the lack of experimental systems where the cortex can be investigated independently from other organelles, little is known about its composition, assembly, and homeostasis. Here, we describe novel tools to resolve the composition and regulation of the cortex. We report and validate a protocol for cortex purification based on the separation of cellular blebs. Mass spectrometry analysis of purified cortices provides a first extensive list of cortical components. To assess the function of identified proteins, we design an automated imaging assay for precise quantification of cortical actomyosin assembly dynamics. We show subtle changes in cortex assembly dynamics upon depletion of the identified cortical component profilin. Our widely applicable integrated method paves the way for systems‐level investigations of the actomyosin cortex and its regulation during morphogenesis. © 2013 Wiley Periodicals, Inc.
Improving performance by bridging the semantic gap between multi-queue SSD and I/O virtualization framework

Virtualization has become one of the most helpful techniques, and today it is prevalent in several computing environments including desktops, data-centers, and enterprises. However, an I/O scalability issue in virtualized environments still needs to be addressed because I/O layers are implemented to be oblivious to the I/O behaviors on virtual machines (VM). In particular, when a multi-queue solid state drive (SSD) is used as a secondary storage, each VM reveals semantic gap that degrades the overall performance of the VM by up to 74%. This is due to two key problems. First, the multi-queue SSD accelerates the possibility of lock contentions. Second, even though both the host machine and the multi-queue SSD provide multiple I/O queues for I/O parallelism, existing Virtio-Blk-Data-Plane supports only one I/O queue by an I/O thread for submitting all I/O requests. In this paper, we propose a novel approach, including the design of virtual CPU (vCPU)-dedicated queues and I/O threads, which efficiently distributes the lock contentions and addresses the parallelism issue of Virtio-Blk-Data-Plane in virtualized environments. We design our approach based on the above principle, which allocates a dedicated queue and an I/O thread for each vCPU to reduce the semantic gap. We also implement our approach based on Linux 3.17, and modify both the Virtio-Blk frontend driver of guest OS and the Virtio-Blk backend driver of Quick Emulator (QEMU) 2.1. 2. Our experimental results with various I/O traces clearly show that our design improves the I/O operations per second (IOPS) in virtualized environments by up to 167% over existing QEMU.
Governance and regulation of urban bus transportation: Using partial privatization to achieve the better of two worlds

Mixed delivery of public services is gaining increasing attention as a way for public managers to avoid deciding between a purely public and purely private delivery system. The unusual coexistence of public and private operators in the urban bus market in Barcelona provides an interesting context in which to analyze the challenges and opportunities posed by this system. Competition for concessions among private operators and the regulation of concessionaires generate incentives to improve efficiency and quality. Furthermore, partial privatization increases the efficiency and feasibility of public operators. In fact, competitive bidding is effective in disciplining private operators and increasing the regulators' bargaining power over both public and private firms. The reform implemented in Barcelona offers an interesting insight into all metropolitan areas that are in a position to create a number of separate concessions large enough to benefit from economies of density.
Present Situation and Prospect of Medical Knowledge Based Systems in German-speaking Countries

Summary Background: After a decrease of interest in classical medical expert systems, the publication activity concerning the medical application of Artificial Intelligence and the interest in medical decision support have markedly increased. Nonetheless, no systematic exploratory study has yet been carried out, which directly considers the actual fields of applications, exemplary approaches, obstacles, challenges, and future prospect as seen by pioneering users and developers in a given region. Objectives: This paper reports the results of an online survey designed to fill this gap with the “Knowledge Based Systems” working group of the German Society for Medical Informatics, Biometry and Epidemiology (GMDS) in 2010. Methods: The survey was based on an online questionnaire (5 single and multiple choice questions, 8 Likert-scaled items, 7 free text questions) consented to by the working group. The answers were analyzed by descriptive statistics and a qualitative analysis (bottom-up coding). All academic institutions of Medical Informatics in the German-speaking countries and contributors reporting KBS-related projects at the relevant scientific conferences and in a journal specialized in the field were invited to participate. Results: The survey reached a response rate of 33.4%. The results show a gap between the reported obstacles of medical KBS (mainly low acceptance and rare use in clinical practice) and their future prospect as stated by the participants. Problems previously discussed in the literature like low acceptance, integration, and sustainability of KBS projects were confirmed. The current situation was characterized by naming exemplary existing systems and specifying promising fields of application. Conclusions: The field of KBS in medicine is more diversified and has evolved beyond expectations in the German-speaking countries.