Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel function of STAT1β in B cells: induction of cell death by a mechanism different from that of STAT1α

Alternate splicing of STAT1 produces two isoforms: α, known as the active form, and β, previously shown to act as a dominant‐negative factor. Most studies have dealt with STAT1α, showing its involvement in cell growth control and cell death. To examine the specific function of either isoform in cell death, a naturally STAT1‐deficient human B cell line was transfected to express STAT1α or STAT1β. STAT1α, expressed alone, enhanced cell death, potentiated the fludarabine‐induced apoptosis, and enhanced the nuclear location, the phosphorylation, and the transcriptional activity of p53. Unexpectedly, STAT1β, expressed alone, induced cell death through a mechanism that was independent of the nuclear function of p53. Indeed, in STAT1β‐expressing B cells, p53 was stricktly cytoplasmic where it formed clusters, and there was no induction of the transcriptional activity of p53. These data reveal a novel role of STAT1β in programmed cell death, which is independent of p53.
Comparison of skin microvascular reactivity with hemostatic markers of endothelial dysfunction and damage in type 2 diabetes

Aim: Patients with non-insulin-dependent diabetes mellitus (NIDDM) are at increased cardiovascular risk due to an accelerated atherosclerotic process. The present study aimed to compare skin microvascular function, pulse wave velocity (PWV), and a variety of hemostatic markers of endothelium injury [von Willebrand factor (vWF), plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (t-PA), tissue factor pathway inhibitor (TFPI), and the soluble form of thrombomodulin (s-TM)] in patients with NIDDM. Methods: 54 patients with NIDDM and 38 sex- and age-matched controls were studied. 27 diabetics had no overt micro- and/or macrovascular complications, while the remainder had either or both. The forearm skin blood flow was assessed by laser-Doppler imaging, which allowed the measurement of the response to iontophoretically applied acetylcholine (endothelium-dependent vasodilation) and sodium nitroprusside (endothelium-independent vasodilation), as well as the reactive hyperemia triggered by the transient occlusion of the circulation. Results: Both endothelial and non-endothelial reactivity were significantly blunted in diabetics, regardless of the presence or the absence of vascular complications. Plasma vWF, TFPI and s-TM levels were significantly increased compared with controls only in patients exhibiting vascular complications. Concentrations of t-PA and PAI-1 were significantly increased in the two groups of diabetics versus controls. Conclusion: In NIDDM, both endothelium-dependent and -independent microvascular skin reactivity are impaired, whether or not underlying vascular complications exist. It also appears that microvascular endothelial dysfunction is not necessarily associated in NIDDM with increased circulating levels of hemostatic markers of endothelial damage known to reflect a hypercoagulable state.
Towards an evaluation of graphical user interfaces aesthetics based on metrics

The graphical user interface (GUI) of an interactive system is nowadays the most frequently used interaction modality. While the contents are of high importance, the Look and Feel is an equally essential factor determining the GUI quality that is impacted by several determinants such as but not limited to aesthetics, pleasurability, fun, etc. Therefore, GUIs aesthetics is a potential element to focus on in order to facilitate communication between device and user. On that basis, one question that comes up is: “Is it possible to evaluate the quality of a GUI by estimating its aesthetics through a series of measurable geometric metrics? ”. This paper suggests possible directions to address the previous question by, first, introducing a simplifying model of GUIs aesthetics that captures aesthetics aspects and regions-related metrics. In a second phase, a methodology for the evaluation of GUIs aesthetics is defined based on the underlying model. The paper finally puts forwards a model-based implementation of the aforementioned methodology in the form of a web service tool for metrics-based evaluation of GUIs and discuss the results of a survey on users aesthetics perceptions.
Persistent neuromuscular and neurophysiologic abnormalities in long-term survivors of prolonged critical illness*

ObjectiveTo establish the prevalence, clinical characteristics, and electrophysiologic features of residual neuromuscular dysfunction after prolonged critical illness. DesignProspective follow-up study of survivors of prolonged critical illness. SettingA university hospital and two district general hospitals in the UK. PatientsThe study occurred for a period of 5 yrs. All patients during that time who were in the intensive care unit for >28 days were entered in the study. Measurements and Main ResultsAt follow-up, length of intensive care unit and hospital stay, duration of mechanical ventilation and admission Acute Physiology and Chronic Health Evaluation II score were recorded from the case notes. A clinical history was obtained, a Barthel Index disability score was calculated, and a full neurologic examination was performed. Nerve conduction studies, needle electromyography, single-fiber electromyography and thermal thresholds were performed. A total of 195 patients were identified. There were 86 survivors, of whom 47 could be contacted and 22 consented to be studied. The median time from intensive care unit discharge to follow-up was 43 months (range, 12–57 months). All gave a clear history of severe weakness and functional impairment after hospital discharge and, in all, recovery was prolonged. Motor or sensory deficits were present on clinical examination in 59% of the patients studied. Common peroneal nerve palsy was present in two patients. A total of 21 of 22 (95%) patients had electromyographic evidence of chronic partial denervation at follow-up, findings indicative of a preceding axonal neuropathy. The single-fiber electromyographic studies were also consistent with a preceding motor neuropathy. ConclusionSevere weakness requiring prolonged rehabilitation and abnormal clinical neurologic findings are extremely common in survivors of protracted critical illness. Neurophysiologic evidence of chronic partial denervation of muscle consistent with previous critical illness polyneuropathy is almost invariable and can be found up to 5 yrs after intensive care unit discharge in >90% of these long-stay patients. Evidence of myopathy is unusual. These findings have important implications for the management and rehabilitation of intensive care survivors.
A compact spectrometer based on a micromachined torsional mirror device

We describe a spectrometer employing a microelectromechanical system (MEMS). A torsional micromirror device, which was originally designed for laser scanner applications, has been modified to act as a diffraction grating in a Czerny-Turner setup. This spectrometer system combines the working principle of a scanning monochromator with the compactness of a microsystem. The micromirror device is operated in a harmonic scanning mode at a resonant frequency of 500 Hz, enabling the system to acquire a spectrum per half cycle in one millisecond. The spectra of a calibration source in the visible and near-infrared spectral range as well as simulations concerning the optical system demonstrate the performance of the spectrometer.
Pilot Study of a New Adjustable Thermoplastic Mandibular Advancement Device for the Management of Obstructive Sleep Apnoea-Hypopnoea Syndrome: A Brief Research Letter

Background: Prefabricated adjustable thermoplastic mandibular advancement devices (PAT-MADs) are a practical short-term treatment for obstructive sleep apnoea-hypopnoea syndrome (OSAHS) in patients who have failed or refused continuous positive airway pressure (CPAP) therapy. Objective: To assess the effectiveness of a new professionally-fitted PAT-MAD in patients with OSAHS in Morocco. Method: Twenty-four adults with mild, moderate or severe OSAHS were fitted with the PAT-MAD (BluePro®; BlueSom, France). Respiratory parameters (apnoea-hypopnoea index (AHI), oxygen desaturation index (ODI)) and daytime sleepiness using the Epworth Sleepiness scale (ESS) were assessed before and after treatment. Adverse events were recorded. Results: Mean treatment duration was 106.3 ± 73.4 days. Mean AHI score decreased from 21.4 ± 7.4 to 9.3 ± 4.1 after treatment (p<0.0001) (mean reduction of 57.0 ± 12.3%). Mean ESS and ODI also decreased at EOS (from 10.4 ± 2.8 to 7.3 ± 2.3, mean reduction 30.3 ± 12.2%, p=0.0001; and 7.0 ± 6.9 to 4.7 ± 4.0, mean reduction 30.5 ± 25.0%, p=0.2, respectively). Treatment was considered to have been successful in 22 patients (91.7%) who had mild OSAHS or an AHI score of ≤5 at the end of the study. The device was well-tolerated. Conclusion: This new PAT-MAD appears to be effective at reducing respiratory parameters and improving daytime alertness in patients with OSAHS. Long term studies in a larger number of patients are warranted to assess the long-term efficacy, retention and side-effects of this device.
Endoscopic and Quality‐of‐Life Outcomes After Revision Endoscopic Sinus Surgery

Objectives: First, to examine the impact of endoscopic sinus surgery (ESS) on endoscopic and quality‐of‐life (QOL) outcomes after revision ESS as compared to primary ESS. Second, to evaluate whether or not other risk factors and/or co‐morbidities influence the relationship between revision surgery status and outcomes of ESS.
A Longitudinal Analysis of Positive Psychological Constructs and Emotions on Stress, Anxiety, and Well-Being

Two studies were conducted including one involving a longitudinal research design to understand better the influential role of the positive psychological capacities of hope, efficacy, optimism, resilience, as well as positive emotions on individual stress, anxiety, and well-being. Study 1 (N = 1,316) was conducted to validate a hypothesized relationship between the positive capacities and well-being. Next, in Study 2 (N = 172), data were collected from participants at 12 points in time over 4 months and random coefficient modeling was used to test hypotheses between variables in a cognitive mediational theoretical framework. Results suggest positive psychological capacities can be a source of positive emotions. In addition, positive emotions and stress mediate the relationship between positive psychological capacities and well-being. A discussion of implications and future research conclude the article.
A Reliable and Valid Weighted Scoring Instrument for Use in Grading APA-Style Empirical Research Report

The scoring instrument described in this article is based on a deconstruction of the seven sections of an American Psychological Association (APA)-style empirical research report into a set of learning outcomes divided into content-, expression-, and format-related categories. A double-weighting scheme used to score the report yields a final grade that reflects the relative importance of outcomes in each category and the differential contribution of each section of the report to the report as a whole. The scores produced by the instrument are reliable between and within raters and significantly correlated with students’ cumulative grade point averages. The author hopes the instrument can provide a useful framework for scoring any set of learning outcomes an instructor defines as the essential elements of an APA-style research report.
Genetic and nucleotide sequence analysis of the gene htdA, which regulates conjugal transfer of IncHI plasmids

IncHI plasmids are naturally repressed for conjugative transfer and do not allow efficient propagation of the IncH pilus-specific phage Hgal. Transposons Tn7, Tn5, and TnlacZ were inserted into IncHI plasmids R478, R477-1, and R27, respectively, leading to the isolation of several plasmid mutants which exhibited increased levels of transfer and also permitted good lysis with phage Hgal. A 4.3-kb HindIII fragment from R478 reversed both phenotypic effects of derepression for the R477-1::Tn5 and the R478::Tn7 derivatives, pKFW99 and pKFW100, respectively. Exonuclease III deletions of this fragment and nucleotide sequence analysis indicated that the gene responsible for transfer repression, named here htdA, encoded a polypeptide of 150 amino acids. Cloning and sequence analysis of pDT2454 (R27::TnlacZ) revealed that the transposon had inserted into an open reading frame (ORF) which had an 83% amino acid identity with the R478 htdA gene. Maxicell analysis showed both the R27 and R478 HtdA products had molecular masses of 19.9 kDa. Conjugation experiments showed that the cloned htdA determinants caused a significant reduction of the transfer frequencies of wild-type R478 and R27 plasmids. Examination of both R478 derepressed mutants, pKFW100 and pKFW101, indicated that both transposon insertions occurred upstream of the htdA ORF. The results suggest that HtdA is a regulatory component of IncH plasmid transfer and also show that the region upstream of the htdA ORF is involved in transfer repression. The locations of the htdA determinants were identified on the plasmid maps of R27 and R478.
Distribution of vasotocin- and vasoactive intestinal peptide-like immunoreactivity in the brain of blue tit (Cyanistes coeruleus)

Blue tits (Cyanistes coeruleus) are songbirds, used as model animals in numerous studies covering a wide field of research. Nevertheless, the distribution of neuropeptides in the brain of this avian species remains largely unknown. Here we present some of the first results on distribution of Vasotocine (AVT) and Vasoactive intestinal peptide (VIP) in the brain of males and females of this songbird species, using immunohistochemistry mapping. The bulk of AVT-like cells are found in the hypothalamic supraoptic, paraventricular and suprachiasmatic nuclei, bed nucleus of the stria terminalis, and along the lateral forebrain bundle. Most AVT-like fibers course toward the median eminence, some reaching the arcopallium, and lateral septum. Further terminal fields occur in the dorsal thalamus, ventral tegmental area and pretectal area. Most VIP-like cells are in the lateral septal organ and arcuate nucleus. VIP-like fibers are distributed extensively in the hypothalamus, preoptic area, lateral septum, diagonal band of Broca. They are also found in the bed nucleus of the stria terminalis, amygdaloid nucleus of taenia, robust nucleus of the arcopallium, caudo-ventral hyperpallium, nucleus accumbens and the brainstem. Taken together, these results suggest that both AVT and VIP immunoreactive structures show similar distribution to other avian species, emphasizing evolutionary conservatism in the history of vertebrates. The current study may enable future investigation into the localization of AVT and VIP, in relation to behavioral and ecological traits in the brain of tit species.
Stimulated bacterioplankton growth and selection for certain bacterial taxa in the vicinity of the ctenophore Mnemiopsis leidyi

Episodic blooms of voracious gelatinous zooplankton, such as the ctenophore Mnemiopsis leidyi, affect pools of inorganic nutrients and dissolved organic carbon by intensive grazing activities and mucus release. This will potentially influence bacterioplankton activity and community composition, at least at local scales; however, available studies on this are scarce. In the present study we examined effects of M. leidyi on bacterioplankton growth and composition in incubation experiments. Moreover, we examined community composition of bacteria associated with the surface and gut of M. leidyi. High release of ammonium and high bacterial growth was observed in the treatments with M. leidyi relative to controls. Deep 454 pyrosequencing of 16 S rRNA genes showed specific bacterial communities in treatments with M. leidyi as well as specific communities associated with M. leidyi tissue and gut. In particular, members of Flavobacteriaceae were associated with M. leidyi. Our study shows that M. leidyi influences bacterioplankton activity and community composition in the vicinity of the jellyfish. In particular during temporary aggregations of jellyfish, these local zones of high bacterial growth may contribute significantly to the spatial heterogeneity of bacterioplankton activity and community composition in the sea.
The double-edged sword of the mammalian oocyte--advantages, drawbacks and approaches for basic and clinical analysis at the single cell level.

Oocytes are usually the largest cells in the body and as such offer unique opportunities for single-cell analysis. Unfortunately, these cells are also some of the rarest in the mammalian female, usually necessitating single-cell analysis. In cases of infertility in humans, determining the quality of the oocyte is often restricted to a morphological analysis or to the study of cellular behaviors in the developing embryo. Minimally invasive approaches could greatly assist the clinician to prioritize oocytes for fertilization or following fertilization, which embryo to transfer back into the woman. Transcriptomics of human and mouse oocytes may have great utility, and recently it was learned that the polar body faithfully reflects the transcript prevalence in the oocyte. The polar body may thus serve as a minimally invasive proxy for an oocyte in the clinic. In the mouse, the transcriptomes of oocytes from mice of the same strain are markedly similar; no significant differences are apparent in transcript prevalence or identity. In human oocytes however, the transcript pool is highly variable. This is likely the result of different histories of each oocyte, in the age of the donor woman, the different hormonal exposures and the prolonged time from specification of the primary oocyte to the fully grown and ovulated egg. This variability in human oocytes also emphasizes the need for cell-by-cell analysis of the oocytes in vitro; which oocytes have a better potential for fertilization and development? To this end, new imaging capabilities arebeing employed. Forexample, a single-cell analytical device for oocytes (the simple perfusion apparatus, or SPA) enables investigators to load multiple oocytes in individual wells, to visualize them on the microscope and to use controlled temperature and media flow by perfusion for optimal clinical applications. Recently, developed Raman microspectroscopy approaches suggest that this imaging modality may enable more in-depth analysis of the molecular characteristics of an oocyte that, in combination with the SPA and transcriptomic approaches, might assist the clinician to prioritize more effectively human oocytes and embryos for transfer into women. This review is intended to update the reader on the status of the examination of single oocytes from a variety of approaches and to emphasize areas that may be primed for advancement in the near future.
Type II Secretion System Secretin PulD Localizes in Clusters in the Escherichia coli Outer Membrane

ABSTRACT The cellular localization of a chimera formed by fusing a monomeric red fluorescent protein to the C terminus of the Klebsiella oxytoca type II secretion system outer membrane secretin PulD (PulD-mCherry) in Escherichia coli was determined in vivo by fluorescence microscopy. Like PulD, PulD-mCherry formed sodium dodecyl sulfate- and heat-resistant multimers and was functional in pullulanase secretion. Chromosome-encoded PulD-mCherry formed fluorescent foci on the periphery of the cell in the presence of high (plasmid-encoded) levels of its cognate chaperone, the pilotin PulS. Subcellular fractionation demonstrated that the chimera was located exclusively in the outer membrane under these circumstances. A similar localization pattern was observed by fluorescence microscopy of fixed cells treated with green fluorescent protein-tagged affitin, which binds with high affinity to an epitope in the N-terminal region of PulD. At lower levels of (chromosome-encoded) PulS, PulD-mCherry was less stable, was located mainly in the inner membrane, from which it could not be solubilized with urea, and did not induce the phage shock response, unlike PulD in the absence of PulS. The fluorescence pattern of PulD-mCherry under these conditions was similar to that observed when PulS levels were high. The complete absence of PulS caused the appearance of bright and almost exclusively polar fluorescent foci.
High Performance OCR for Camera-Captured Blurred Documents with LSTM Networks

Documents are routinely captured by digital cameras in today's age owing to the availability of high quality cameras in smart phones. However, recognition of camera-captured documents is substantially more challenging as compared to traditional flat bed scanned documents due to the distortions introduced by the cameras. One of the major performancelimiting artifacts is the motion and out-of-focus blur that is often induced in the document during the capturing process. Existing approaches try to detect presence of blur in the document to inform the user for re-capturing the image. This paper reports, for the first time, an Optical Character Recognition (OCR) system that can directly recognize blurred documents on which the stateof-the-art OCR systems are unable to provide usable results. Our presented system is based on the Long Short-Term Memory (LSTM) networks and has shown promising character recognition results on both the motion-blurred and out-of-focus blurred images. One important feature of this work is that the LSTM networks have been applied directly to the gray-scale document images to avoid error-prone binarization of blurred documents. Experiments are conducted on publicly available SmartDoc-QA dataset that contains a wide variety of image blur degradations. Our presented system achieves 12.3% character error rate on the test documents, which is an over three-fold reduction in the error rate (38.9%) of the best-performing contemporary OCR system (ABBYY Fine Reader) on the same data.
GloptiPoly: global optimization over polynomials with Matlab and SeDuMi

GloptiPoly is a Matlab/SeDuMi add-on to build and solve convex linear matrix inequality relaxations of the (generally non-convex) global optimization problem of minimizing a multivariable polynomial function subject to polynomial inequality, equality or integer constraints. It generates a series of lower bounds monotonically converging to the global optimum. Global optimality is detected and isolated optimal solutions are extracted automatically. Numerical experiments show that for most of the small- and medium-scale problems described in the literature, the global optimum is reached at low computational cost.
Stress perception and production in German stress clash environments

Several production and perception experiments have been carried out in order to investigate, whether stress clash environments in A(BC)-compounds really trigger a stress shift or a destressing of the secondary stress or not. The results indicate that stress shifting is rather rare than common in German. If it occurs, it manifests itself both on a production and perception level. This situation remains stable, no matter if listeners are presented the full acoustic signal or only delexicalised stimuli. The only environment where stress shift tends to take place more often are words consisting of four syllables. These allow for placement of an additional foot. Stress clashes in trisyllabic compounds are often handled by a destressing of the secondary accent as an alternative to stress shift. However, in the majority of stress clash environments, listeners did perceive the primary and secondary stress in the immediate neighbourhood. Obvi- ously, a stress clash creates less of a problem in German than it does in other Germanic languages.