Miracle-dz/UniFAR-FTUs huggingface dataset:
POLYMER BRUSHES INFILTRATED BY NANOPARTICLES AND APPLICATIONS TO THE NUCLEAR PORE COMPLEX

Systems of grafted polymers in the presence of additives are useful in a variety of contexts including industrial applications, solar cells, organic electronics, drug delivery, and nucleocytoplasmic transport. In this thesis, we will consider the morphologies that polymer brushes attain when exposed to a solution of additives (which we generically term ``nanoparticles"), particularly when those nanparticles interact attractively with the polymers. We find that nanoparticles of this type can have a dramatic effect on the height of the polymer chains above the grafting surface, and they can induce highly non-uniform morphologies, including ones in which a dense layer of nanoparticles and monomers forms near the grafting surface. We consider especially the relevance of the system to several experiments performed on biopolymers in the nuclear pore complex when they interact attractively with transport factors that regulate nucleocytoplasmic transport. We find that, although these experiments appear to give inconsistent results, the inconsistencies can be reconciled through two simple models: the Alexander-de Gennes polymer brush, and the Milner-Witten-Cates polymer brush. Our findings should contribute to the understanding of the nuclear pore complex in that experiments can be better understood in the context of their relevant control parameters.
Online Bipartite Perfect Matching With Augmentations

In this paper, we study an online bipartite matching problem, motivated by applications in wireless communication, content delivery, and job scheduling. In our problem, we have a bipartite graph G between n clients and n servers, which represents the servers to which each client can connect. Although the edges of G are unknown at the start, we learn the graph over time, as each client arrives and requests to be matched to a server. As each client arrives, she reveals the servers to which she can connect, and the goal of the algorithm is to maintain a matching between the clients who have arrived and the servers. Assuming that G has a perfect matching which allows all clients to be matched to servers, the goal of the online algorithm is to minimize the switching cost, the total number of times a client needs to switch servers in order to maintain a matching at all times. Although there are no known algorithms which are guaranteed to yield switching cost better than the trivial O(n 2 ) in the worst case, we show that the switching cost can be much lower in three natural settings. In our first result, we show that for any arbitrary graph G with a perfect matching, if the clients arrive in random order, then the total switching cost is only O(n log n) with high probability. This bound is tight, as we show an example where the switching cost is Omega(n log n) in expectation. In our second result, we show that if each client has edges to Theta(log n) uniformly random servers, then the total switching cost is even better; in this case, it is only O(n) with high probability, and we also have a lower bound of Omega(n/log n). In terms of the number of edges needed for each client, our result is tight, since Omega(log n) edges are needed to guarantee a perfect matching in G with high probability. In our last result, we derive the first algorithm known to yield total cost O(n log n), given that the underlying graph G is a forest. This is the first result known to match the existing lower bound for forests, which shows that any online algorithm must have switching cost Omega(n log n), even when G is restricted to be a forest.
Gender differences in insulin action after a single bout of exercise.

Effects of a single exercise bout on insulin action were compared in men (n = 10) and women (n = 10). On an exercise day, subjects cycled for 90 min at 85% lactate threshold, whereas on a rest (control) day, they remained semirecumbent. The period of exercise, or rest, was followed by a 3-h hyperinsulinemic-euglycemic clamp (30 mU.m(-2). min(-1)) and indirect calorimetry. Glucose kinetics were measured isotopically by using an infusion of [6,6-2H2]glucose. Glucose infusion rate (GIR) during the clamp on the rest day was not different between the genders. However, GIR on the exercise day was significantly lower in men compared with women (P = 0.01). This was mainly due to a significantly lower glucose rate of disappearance in men compared with women (P = 0.05), whereas no differences were observed in the endogenous glucose rate of appearance. Nonprotein respiratory quotient (NPRQ) increased significantly during the clamp from preclamp measurements in men and women on the rest day (P < 0.01). Exercise abolished the increase in NPRQ seen during the clamp on the rest day and tended to decrease NPRQ in men. Our results indicate the following: 1) exercise abolishes the usual increase in NPRQ observed during a hyperinsulinemic-euglycemic clamp in both genders, 2) men exhibit relatively lower whole body insulin action in the 3-4 h after exercise compared with women, and 3) gender differences in insulin action may be explained by a lower glucose rate of disappearance in the men after acute exercise. Together, these data imply gender differences in insulin action postexercise exist in peripheral tissues and not in liver.
Sensitivities of Nasal and Rectal Swabs for Detection of Methicillin-Resistant Staphylococcus aureus Colonization in an Active Surveillance Program

ABSTRACT All medical and high-risk surgical patients were screened for methicillin-resistant Staphylococcus aureus colonization over 3.5 years. The sensitivities of nasal and rectal swabs were 68% and 62%, respectively. Naris and open-skin-site swabs detected 467 (74%) of 627 adult carriers identified. Rectal swabs detected an additional 160 (26%) carriers.
Mucosal secretion and expression of basic fibroblast growth factor in patients with collagenous colitis

OBJECTIVES:Collagenous colitis (CC), a condition of unknown ethiology and pathophysiology, is characterized by watery diarrhea and increased amounts of collagen in the colonic mucosa. Basic fibroblast growth factor (bFGF) is a potent multifunctional growth factor that stimulates proliferation and differentiation of fibroblasts that was recently detected in high intraluminal concentrations in patients with ulcerative colitis. In this study we examined the secretion and expression of bFGF in the colorectal mucosa of patients with CC.METHODS:Ten CC patients and 10 control patients underwent colonoscope-based segmental perfusion of the descending colon and rectum. The concentrations of bFGF in perfusate and serum were determined by immunochemical methods, and the expression of bFGF was analyzed immunohistochemically in biopsy samples from colonic mucosa.RESULTS:The median concentrations of bFGF in perfusates from the descending colon and rectum were increased 4-fold in CC patients compared with control patients. The median concentration of bFGF in serum was not significantly different in patients and controls. Immunohistochemical staining of bFGF was located in the colorectal epithelial cells and in exsudate on the luminal side of these cells. In the lamina propria, inflammatory cells and fibroblasts contained bFGF. There were no differences in the intensity of bFGF staining in surface epithelium or lamina propria between patients and controls.CONCLUSION:Local investigation of the colorectal mucosa seems to be crucial when studying the pathophysiology of CC. Increased colorectal mucosal secretion of bFGF in patients with CC may promote differentiation of fibroblasts and thereby lead to increased subepithelial collagen deposition.
The long road to steady state in gas exchange: metabolic and ventilatory responses to hypercapnia and hypoxia in Cuvier's dwarf caiman

ABSTRACT Animals with intermittent lung ventilation and those exposed to hypoxia and hypercapnia will experience fluctuations in the bodily O2 and CO2 stores, but the magnitude and duration of these changes are not well understood amongst ectotherms. Using the changes in the respiratory exchange ratio (RER; CO2 excretion divided by O2 uptake) as a proxy for changes in bodily gas stores, we quantified time constants in response to hypoxia and hypercapnia in Cuvier's dwarf caiman. We confirm distinct and prolonged changes in RER during and after exposure to hypoxia or hypercapnia. Gas exchange transients were evaluated in reference to predictions from a two-compartment model of CO2 exchange to quantify the effects of the levels of hypoxia and hypercapnia, duration of hypercapnia (30–300 min) and body temperature (23 versus 33°C). For hypercapnia, the transients could be adequately fitted by two-phase exponential functions, and slow time constants (after 300 min hypercapnia) concurred reasonably well with modelling predictions. The slow time constants for the decays after hypercapnia were not affected by the level of hypercapnia, but they increased (especially at 23°C) with exposure time, possibly indicating a temporal and slow recruitment of tissues for CO2 storage. In contrast to modelling predictions, elevated body temperature did not reduce the time constants, probably reflecting similar ventilation rates in transients at 23 and 33°C. Our study reveals that attainment of steady state for gas exchange requires considerable time and this has important implications for designing experimental protocols when studying ventilatory control and conducting respirometry. Summary: Attainment of a steady state in gas exchange upon and following hypoxia and hypercapnia in reptiles may take a prolonged time, especially if blood flow and ventilation rates are low.
PhcS Represses Gratuitous Expression of Phenol-Metabolizing Enzymes in Comamonas testosteroni R5

ABSTRACT We identified an open reading frame, designatedphcS, downstream of the transcriptional activator gene (phcR) for the expression of multicomponent phenol hydroxylase (mPH) in Comamonas testosteroni R5. The deduced product of phcS was homologous to AphS ofC. testosteroni TA441, which belongs to the GntR family of transcriptional regulators. The transformation of Pseudomonas aeruginosa PAO1c (phenol negative, catechol positive) with pROR502 containingphcR and the mPH genes conferred the ability to grow on phenol, while transformation with pROR504 containingphcS, phcR, and mPH genes did not confer this ability. The disruption of phcS in strain R5 had no effect on its phenol-oxygenating activity in a chemostat culture with phenol. The phenol-oxygenating activity was not expressed in strain R5 grown in a chemostat with acetate. In contrast, the phenol-oxygenating activity in the strain with a knockoutphcS gene when grown in a chemostat with acetate as the limiting growth factor was 66% of that obtained in phenol-grown cells of the strain with a knockout in the phcSgene. The disruption of phcS and/orphcR and the complementation in trans of these defects confirm that PhcS is a trans-acting repressor and that the unfavorable expression of mPH in thephcS knockout cells grown on acetate requires PhcR. These results show that the PhcS protein repressed the gratuitous expression of phenol-metabolizing enzymes in the absence of the genuine substrate and that strain R5 acted by an unknown mechanism in which the PhcS-mediated repression was overcome in the presence of the pathway substrate.
Liquid-based cervical cytologic smear study and conventional Papanicolaou smears: a metaanalysis of prospective studies comparing cytologic diagnosis and sample adequacy.

OBJECTIVE We sought to evaluate the cytologic diagnosis and sample adequacy of the liquid-based cervical cytologic smear (ThinPrep) compared with that of the conventional Papanicolaou smear. STUDY DESIGN Prospective studies of ThinPrep and conventional Papanicolaou smears were analyzed for cytologic diagnosis and sample adequacy. Computerized databases, references in published studies, and index reviews published in English were used to identify direct-to-vial and split-sample clinical trials of cervical smears performed by conventional and liquid-based techniques. Only published studies that used the Bethesda system nomenclature with clearly documented outcome data were included. Each trial was assessed for the quality of its method, inclusion and exclusion criteria, adequacy of randomization, sampling protocols, definition of outcome, and statistical analyses. RESULTS Twenty-five studies met inclusion criteria for this review. Odds ratios with 95% confidence intervals were calculated for each outcome. Estimates of odds ratios and risk differences for dichotomous outcomes were calculated by use of random and fixed-effects models. Homogeneity was tested across the studies. Results indicate that the ThinPrep test is as good as or superior to the conventional Papanicolaou smear in diagnosing uterine cervical premalignant abnormalities. Also the ThinPrep test provides improved sample adequacy when compared with the conventional Papanicolaou test. CONCLUSION The ThinPrep test improved sample adequacy and led to improved diagnosis of low-grade and high-grade squamous intraepithelial lesions. However, there is no difference in the rate of atypical cells of undetermined significance diagnosis between ThinPrep and conventional smear groups. The added cost of ThinPrep cytologic screening and, hence, its cost-effectiveness are not evaluated in this study.
Reduction in behavior problems with omega-3 supplementation in children aged 8-16 years: a randomized, double-blind, placebo-controlled, stratified, parallel-group trial.

BACKGROUND While limited evidence suggests that omega-3 supplementation may reduce antisocial behavior in children, studies have not reported on posttreatment follow-up and most treatment periods have been of short duration. This study tests the hypothesis that omega-3 supplementation over 6 months will reduce behavior problems in children both at the end of treatment and at 6 months post treatment. METHODS In this randomized, double-blind, placebo-controlled, stratified, parallel-group trial, a community sample of 8-16 year old children were randomized into a treatment group (N = 100) and a placebo-control group (N = 100). The supplementation consisted of a fruit drink containing 1 g/day of omega-3 or a placebo consisting of the same fruit drink without omega-3. Participants, caregivers, and research assistants were blinded to group assignment. The primary outcome measures of externalizing and internalizing behavior problems were reported by both caregivers and their children in a laboratory setting at 0 months (baseline), 6 months (end of treatment) and 12 months (6 months post treatment), together with the secondary outcome measures of parental antisocial behavior. Data were analyzed on an intention-to-treat basis including all participants. TRIAL REGISTRATION ClinicalTrials.gov: http://clinicaltrials.gov/ct2/show/NCT02016079? term=mauritius&rank=2 RESULTS: Significant group × time interactions were observed with the treatment group showing long-term improvements in child behavior problems. The average posttreatment effect size was d = -. 59. Effects were documented for parent reports, but with the exception of proactive and reactive aggression, child-report data were nonsignificant. Parents whose children took omega-3 showed significant posttreatment reductions in their own antisocial and aggressive behavior. This improvement in caregiver behavior partly mediated the improvements observed in child behavior. CONCLUSIONS Findings provide initial evidence that omega-3 supplementation can produce sustained reductions in externalizing and internalizing behavior problems. Results are the first to report improvements in caregiver behavior, and to establish this improvement as a part-mechanism for the efficacy of omega-3.
Visual Performance after Bilateral Implantation of a Four-Haptic Diffractive Toric Multifocal Intraocular Lens in High Myopes

Background. The vision with diffractive toric multifocal intraocular lenses after cataract surgery in long eyes has not been studied previously. Objectives. To report visual performance after bilateral implantation of a diffractive toric multifocal intraocular lens in high myopes. Methods. Prospective, observational case series to include patients with axial length of ≥26 mm and corneal astigmatism of >1 dioptre who underwent bilateral AT LISA 909M implantation. Postoperative examinations included photopic and mesopic distance, intermediate, and near visual acuity; photopic contrast sensitivity; visual symptoms (0–5); satisfaction (1–5); and spectacle independence rate. Results. Twenty-eight eyes (14 patients) were included. Postoperatively, mean photopic monocular uncorrected distance, intermediate, and near visual acuities (logMAR) were 0.12 ± 0.20 (standard deviation), 0.24 ± 0.16, and 0.29 ± 0.21, respectively. Corresponding binocular values were −0.01 ± 0.14, 0.13 ± 0.12, and 0.20 ± 0.19, respectively. One eye (4%) had one-line loss in vision. Under mesopic condition, intermediate vision and near vision decreased significantly (all P ≤ 0.001). Contrast sensitivity at all spatial frequencies did not improve significantly under binocular condition (all P > 0.05). Median scores for halos, night glare, starbursts, and satisfaction were 0.50, 0.00, 0.00, and 4.25, respectively. Ten patients (71%) reported complete spectacle independence. Conclusions. Bilateral implantation of the intraocular lens in high myopes appeared to be safe and achieved good visual performance and high satisfaction.
Cyberbullying detection: a step toward a safer internet yard

As a result of the invention of social networks friendships, relationships and social communications have all gone to a new level with new definitions. One may have hundreds of friends without even seeing their faces. Meanwhile, alongside this transition there is increasing evidence that online social applications have been used by children and adolescents for bullying. State-of-the-art studies in cyberbullying detection have mainly focused on the content of the conversations while largely ignoring the users involved in cyberbullying. We propose that incorporation of the users' information, their characteristics, and post-harassing behaviour, for instance, posting a new status in another social network as a reaction to their bullying experience, will improve the accuracy of cyberbullying detection. Cross-system analyses of the users' behaviour - monitoring their reactions in different online environments - can facilitate this process and provide information that could lead to more accurate detection of cyberbullying.
The future of software

Companies like British Telecommunications whose business is based on the performance of their software systems, should emphasize accessibility, adaptability, transparency, fail-safe operation, and a human face. Software Producing such software is difficult and involves high costs and risks. Adopting the most appropriate methods, technologies, and tools at just the right time is a major problem for the software industry. Recognition of the critical role played by software in so many aspects of society has therefore led us to pursue the following goals: • Forming a vision of software and software development based on the systematic use of expert judgement and peer review; • Establishing the need for a long-term software-development research agenda; and • Outlining a long-term research agenda that helps meet society's future needs for software that is reliable, adaptable, available when needed, and reasonably priced. Predicting the future is a popular pastime in many disciplines. In the field of software development, the February 1997 Communications offered many personal hopes and visions for the future of computer technology [1]. Authors expressed their personal views relating to particular technologies and applications , including databases [2], the Internet [3], and computational humanities [6]. In another personal view of future needs, Stuart Shapiro [5] examined a number of key software technology publications dating from as far back as the 1960s, concluding we need a more pluralistic approach to software engineering, Society is increasingly dependent on large and complex software systems. Indeed, if many of the current predictions about the Y2K computer problem turn out to be accurate, this dependency will be demonstrated dramatically on January 1, 2000. Users need software that meets stringent requirements, supports a range of interaction styles, can be produced quickly, and can be maintained to keep pace with the ever-increasing demand for functional-ity, quality, flexibility, and cost-effectiveness.
When MIMO Control Meets MIMO Communication: A Majorization Condition for Networked Stabilizability

In this paper, we initiate the study of networked stabilization via a MIMO communication scheme between the controller and the plant. Specifically, the communication system is modeled as a MIMO transceiver, which consists of three parts: an encoder, a MIMO channel, and a decoder. In the spirit of MIMO communication, the number of SISO subchannels in the transceiver is often greater than the number of data streams to be transmitted. Moreover, the subchannel capacities are assumed to be fixed a priori. In this case, the encoder/decoder pair gives an additional design freedom on top of the controller, leading to a stabilization problem via coding/control co-design. It turns out that how to take the best advantage of the coding mechanism is quite crucial. From a demand/supply perspective, the design of the coding mechanism boils down to reshaping the demands for communication resource from different control inputs to match the given supplies. We study the problem for the case of AWGN subchannels and fading subchannels, respectively. In both cases, we arrive at a unified necessary and sufficient condition on the capacities of the subchannels under which the coding/control co-design problem is solvable. The condition is given in terms of a majorization type relation. As we go along, systematic procedures are also put forward to implement the coding/control co-design. A numerical example is presented to illustrate our results.
Organ-Specific Protection Against Lipopolysaccharide-Induced Vascular Leak Is Dependent on the Endothelial Protein C Receptor

Objective—To study the role of the endothelial protein C receptor (EPCR) in the modulation of susceptibility to inflammation-induced vascular leak in vivo. Approach and Results—Genetically modified mice with low, <10% EPCR expression (EPCRlow) and control mice were challenged with lipopolysaccharides in a mouse model of endotoxemia. Infrared fluorescence and quantification of albumin-bound Evans Blue in tissues and intravascular plasma volumes were used to assess plasma extravasation. Pair-wise analysis of EPCRlow and control mice matched for sex, age, and weight allowed determination of EPCR-dependent vascular leak. Kidney, lung, and brain were the organs with highest discriminative increased Evans Blue accumulation in EPCRlow versus control mice in response to lipopolysaccharides. Histology of kidney and lung confirmed the EPCR-specific pathology. In addition to severe kidney injury in response to lipopolysaccharides, EPCRlow and anti-EPCR–treated wild-type mice suffered from enhanced albuminuria and profound renal hemorrhage versus controls. Intravascular volume loss at the same extent of weight loss in EPCRlow mice compared with control mice provided proof that plasma leak was the predominant cause of Evans Blue tissue accumulation. Conclusions—This study demonstrates an important protective role for EPCR in vivo against vascular leakage during inflammation and suggests that EPCR-dependent vascular protection is organ-specific.