Miracle-dz/UniFAR-FTUs huggingface dataset:
Endogenous plasma endothelin concentrations and coronary circulation in patients with mild dilated cardiomyopathy

OBJECTIVE To determine whether increased plasma concentrations of endothelin-1 (ET-1) and big endothelin (BET) play a role in the regulation of coronary circulation in patients with idiopathic dilated cardiomyopathy (IDCM). SETTING Tertiary referral centre for cardiac diseases. PATIENTS Fourteen patients (eight male/six female; mean (SD) age 59 (9) years) with IDCM (ejection fraction 36 (9)%) and five normotensive subjects (two male/three female; age 52 (7) years) serving as controls were studied. METHODS Functional status was classified according to New York Heart Association (NYHA) class. Endogenous ET-1 and BET plasma concentrations from the aorta and the coronary sinus were determined by radioimmunoassay. Coronary blood flow, using the inert chromatographic argon method, myocardial oxygen consumption, and coronary sinus oxygen content under basal conditions were determined. RESULTS In the aorta, mean (SD) concentrations of ET-1 (IDCM 0.76 (0.25) v controls 0.31 (0.06) fmol/ml; p = 0.002) and BET (IDCM 3.58 (1.06) vcontrols 2.11 (0.58) fmol/ml; p = 0.014) were increased in patients with IDCM. Aortic ET-1 concentrations correlated positively with NYHA class (r = 0.731; p < 0.001), myocardial oxygen consumption (r = 0.749; p < 0.001), and coronary blood flow (r = 0.645; p = 0.003), but inversely with coronary sinus oxygen content (r = −0.633; p = 0.004), which was significantly decreased in IDCM patients (IDCM 4.68 (1.05)v controls 6.70 (1.06) vol%; p = 0.003). CONCLUSIONS The coronary circulation in patients with IDCM is exposed to an increased endothelin load. ET-1 concentrations correlate with functional deterioration. A decrease of the coronary sinus content of oxygen suggests a mismatch between coronary blood flow and metabolic demand. Thus, ET-1 might be a marker of a disequilibrium between myocardial oxygen demand and coronary blood flow in IDCM.
Dispositions Toward (Collective) Struggle and Educational Resilience in the Inner City: A Case Analysis of Six African-American High School Students

For more than 20 years, researchers have shown that the recognition of a limited opportunity structure on the part of marginal youth circumscribes their optimism toward the future and hence increases their likelihood of disengaging from school. This article, however, focuses on six, low-income, African-American adolescents who expected to realize their ambitions and were high achieving, all the while articulating an acute recognition of how race and class (and, in two cases, gender) operated to constrain the life chances of people like themselves. These students’ familiarity with struggle, including collective struggle, was the only biographical factor which distinguished them from the other respondents in the larger project of which they were a part. Thus, in contrast to the findings of some, their knowledge of struggle did not curtail their academic success but may have contributed to their sense of human agency and facilitated their academic motivation. Because this knowledge derived from their interaction with significant others, this article also maintains that the meanings that arise from immediate experiences and discourses are essential for understanding the diverse ways by which marginal people interpret and respond to their subjugation.
Adaptive LightSlice for Virtual Ray Lights

We speed up the rendering of participating media with Virtual Ray Lights (VRLs) by clustering them in a preprocessing step. A subset of representative VRLs is then sampled from the clustering, which is used for the final rendering. By performing a full variance analysis, we can explicitly estimate the convergence rate of the rendering process and automatically find the locally ideal number of clusters to maximize efficiency. Overall, we report speed-up factors ranging from 13 to 16 compared to unclustered rendering.
The Cullin 3 Ubiquitin Ligase Functions as a Nedd 8-bound Heterodimer

Cullins are members of a family of scaffold proteins that assemble multisubunit ubiquitin ligase complexes to confer substrate specificity for the ubiquitination pathway. Cullin3 (Cul3) forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of the ubiquitin homologue neural-precursor-cellexpressed and developmentally down regulated 8 (Nedd8) near the C terminus of Cul3. Current models suggest that Nedd8 activates cullin complexes by providing a recognition site for a ubiquitin-conjugating enzyme. Based on the following evidence, we propose that Nedd8 activates the BCR ubiquitin ligase by mediating the dimerization of Cul3. First, Cul3 is found as a neddylated heterodimer bound to a BTB domain-containing protein in vivo. Second, the formation of a Cul3 heterodimer is mediated by a Nedd8 molecule, which covalently attaches itself to one Cul3 molecule and binds to the winged-helix B domain at the C terminus of the second Cul3 molecule. Third, complementation experiments revealed that coexpression of two distinct nonfunctional Cul3 mutants can rescue the ubiquitin ligase function of the BCR complex. Likewise, a substrate of the BCR complex binds heterodimeric Cul3, suggesting that the Cul3 complex is active as a dimer. These findings not only provide insight into the architecture of the active BCR complex but also suggest assembly as a regulatory mechanism for activation of all cullin-based ubiquitin ligases.
Hoxd13 expression in the developing limbs of the short‐tailed fruit bat, Carollia perspicillata

Summary Bat forelimbs are highly specialized for sustained flight, providing a unique model to explore the genetic programs that regulate vertebrate limb diversity. Hoxd9–13 genes are important regulators of stylopodium, zeugopodium, and autopodium development and thus evolutionary changes in their expression profiles and biochemical activities may contribute to divergent limb morphologies in vertebrates. We have isolated the genomic region that includes Hoxd12 and Hoxd13 from Carollia perspicillata, the short‐tailed fruit bat. The bat Hoxd13 gene encodes a protein that shares 95% identity with human and mouse HOXD13. The expression pattern of bat Hoxd13 mRNA during limb development was compared with that of mouse. In bat and mouse hindlimbs, the expression patterns of Hoxd13 are relatively similar. However, although the forelimb Hoxd13 expression patterns in both organisms during early limb bud stages are similar, at later stages they diverge; the anterior expression boundary of bat Hoxd13 is posterior‐shifted relative to the mouse. These findings, compared with the Hoxd13 expression profiles of other vertebrates, suggest that divergent Hoxd13 expression patterns may contribute to limb morphological variation.
Menu suggestion: an effective way to improve dietary compliance in peritoneal dialysis patients.

OBJECTIVE Poor compliance with the dietary prescriptions is quite common in dialysis patients. We believe that most of the noncompliance is caused by the patient's poor understanding of the dietary prescription. Therefore, in the present study, we tried to investigate the role of menu suggestion in improving the patient's compliance with the dietary prescription. DESIGN AND SETTING A longitudinal cohort study conducted at an outpatient dialysis clinic. PATIENTS Seventy clinically stable patients on peritoneal dialysis were included in this prospective study during April 1, 2004, to November 31, 2004, in a single center. Patients who had significant cognitive impairment and thus did not understand the food contents during the training course were not eligible for enrollment. INTERVENTION All the patients were randomly assigned to 1 of 2 groups. Group 1 patients received the traditional patient education method. Group 2 patients additionally received individualized menu suggestions based on their food preferences and education on how to exchange the foods at equivalent amounts according to the exchange list. MAIN OUTCOME MEASURES At present, there are no clear optimal dietary protein intake levels for peritoneal dialysis patients. Our experience is that a dietary protein intake level of 0.8 to 1.2 g/kg/d can maintain our patients in a good nutritional status. Thereafter, in this study we prescribed the dietary protein intake level at 0.8 to 1.2 g/kg/d and defined compliance as meeting this target protein intake level. RESULTS There were 35 patients in each group. The compliance was 22.9% in group 1 and 57.1% in group 2 (P < .05). CONCLUSIONS Our study suggests that menu suggestion may be an effective way of improving the compliance with the diet in peritoneal dialysis patients. It improves the patient's understanding of the dietary prescription.
Decreased Expression of Gene Cluster at Chromosome 1q21 Defines Molecular Subgroups of Chemoradiotherapy Response in Esophageal Cancers

Purpose: Resistance to preoperative chemoradiotherapy (CTXRT) in 75% of patients with esophageal adenocarcinoma (EAC) underscores the need for identification of biomarkers of CTXRT response. We previously noted an association between decreased expression of epidermal differentiation complex (EDC) genes S100A2 and SPRR3 at chromosome 1q21 and CTXRT resistance. In the current study, we did an in-depth investigation of the expression of 1q21-1q25 region genes to uncover the role of the EDC and its flanking genes in CTXRT response. Experimental Design: We compared 19 pretreatment EAC specimens with normal squamous mucosa for the expression of 517 genes at chromosome 1q21-1q25 and selected target genes based on their differential expression. Using the pathologic complete-response (pathCR) status of the resected specimens as a representation of CTXRT sensitivity, we assessed the association between the expression of target genes and CTXRT response and clinical outcomes. Results: On the basis of the expression levels of IVL, CRNN, NICE-1, S100A2, and SPPR3, genes within and in close proximity to the EDC, cancers were segregated into high (subgroup I) or low (subgroup II) expressers. Four of the five pathCRs were high expressers. Thus, low expressers, with one exception, were all nonresponders. Patients in subgroup I also had longer survival than those in subgroup II, although this result was not statistically significant owing to the small study number. Conclusions: The expression levels of genes mapping within and close to the EDC define CTXRT response subgroups in EACs.
Macro-level Gender Inequality and the Division of Household Labor in 22 Countries

While most previous studies focus on the effects of individuals' and couples' characteristics on the division of housework, this study argues that macro-level factors are equally important in the dynamics of housework distribution between spouses. Data from the 1994 International Social Survey Programme is used to examine whether macro-level gender inequality limits the effect of individual-level variables (relative resources, time availability, and gender ideology) on the division of housework in 22 industrialized countries. The results show that the equalizing effects of time availability and gender ideology are stronger for women in more egalitarian countries; women in less egalitarian countries benefit less from their individual-level assets. Additional analysis shows that other macro-level factors (economic development, female labor-force participation, gender norms, and welfare regimes) may also influence the division of housework. The results suggest that changes in individual-level factors may not be enough to achieve an equal division of housework without the reduction of macro-level gender inequality.
Development of a Tier 3 Curriculum to Teach Early Literacy Skills

Children with the lowest oral language and early literacy skills at entry to kindergarten are the most vulnerable to poor literacy outcomes. This article describes the programmatic development of a Tier 3 early literacy intervention for preschool children who are most in need of intensive support to achieve early literacy outcomes. The intervention consists of carefully sequenced activities and games to promote early literacy development and is designed to be implemented by an early childhood educator with small groups of one to two children during center time or small-group instruction time in the classroom. Development of, and research on, the intervention followed an iterative process leading to a sequence of studies demonstrating moderate to strong effect sizes for children who qualified for Tier 3 support. Children who received intervention were a diverse group who presented an array of challenging characteristics that required individualization of the intervention. Children progressed at different rates, and some children clearly needed more opportunities to receive the intervention. Further research is needed to investigate factors related to effectiveness of Tier 3 interventions, including characteristics of the intervention, such as duration, time, and group size, as well as the characteristics of children who respond/do not respond.
Insulin-Regulated Trafficking of GLUT4 Requires Ubiquitination

A major consequence of insulin binding its receptor on fat and muscle cells is translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the cell surface where it serves to clear glucose from the bloodstream. Sorting of GLUT4 into its insulin‐sensitive store requires the GGA [Golgi‐localized, γ‐ear‐containing, ADP ribosylation factor (ARF)‐binding] adaptor proteins, but the signal on GLUT4 to direct this sorting step is unknown. Here, we have identified a role for ubiquitination of GLUT4 in this process. We demonstrate that GLUT4 is ubiquitinated in 3T3‐L1 adipocytes, and that a ubiquitin‐resistant version fails to translocate to the cell surface of these cells in response to insulin. Our data support a model in which ubiquitination acts as a signal for the trafficking of GLUT4 from the endosomal/trans‐Golgi network (TGN) system into its intracellular storage compartment, from where it is mobilized to the cell surface in response to insulin.
Selective 5HT2A and 5HT6 receptor antagonists promote sleep in rats.

STUDY OBJECTIVES Serotonin (5-HT) has long been implicated in the control of sleep and wakefulness. This study evaluated the hypnotic efficacy of the 5-HT6 antagonist RO4368554 (RO) and the 5-HT2A receptor antagonist MDL100907 (MDL) relative to zolpidem. DESIGN A randomized, repeated-measures design was utilized in which Wistar rats received intraperitoneal injections of RO (1.0, 3.0, and 10 mg/kg), MDL (0.1, 1.0 and 3.0 mg/kg), zolpidem (10 mg/kg), or vehicle in the middle of the dark (active) period. Electroencephalogram, electromyogram, body temperature (Tb) and locomotor activity were analyzed for 6 hours after injection. MEASUREMENTS AND RESULTS RO, MDL, and zolpidem all produced significant increases in sleep and decreases in waking, compared with vehicle control. All 3 doses of MDL produced more consolidated sleep, increased non-rapid eye movement sleep (NREM) sleep, and increased electroencephalographic delta power during NREM sleep. The highest dose of RO (10.0 mg/kg) produced significant increases in sleep and decreases in waking during hour 2 following dosing. These increases in sleep duration were associated with greater delta power during NREM sleep. ZO Zolpidem induced sleep with the shortest latency and significantly increased NREM sleep and delta power but also suppressed rapid eye movement sleep sleep; in contrast, neither RO nor MDL affected rapid eye movement sleep. Whereas RO did not affect Tb, both zolpidem and MDL reduced Tb relative to vehicle-injected controls. CONCLUSIONS These results support a role for 5-HT2A receptor modulation in NREM sleep and suggest a previously unrecognized role for 5-HT6 receptors in sleep-wake regulation.
Retrospective study of diseases and associated pneumonia type diagnosed in dogs at post-mortem at Veterinary Teaching Hospital, Ibadan, Nigeria

The causes and types of pneumonia in dogs have not been accorded due attention in Nigeria. It is imperative to investigate the incidence and type of pneumonia commonly observed during post-mortem at the Department of Veterinary Pathology arm of the Veterinary Teaching Hospital, Ibadan, Nigeria. This investigation was carried out on 397 archival canine samples for dogs presented for necropsy at the Department of Veterinary Pathology, University of Ibadan, during the periods of 2000 - 2012. The gross diagnosis was obtained from the postmortem records while the specific type of pneumonia was by histopathology of selected lungs tissues, using standard techniques. Descriptive statistics was employed to evaluate the effect of age, sex, breed and the type of pneumonia. Alsatian(29%) and Rottweiler(14.4%) breeds of dogs were the common breeds of dogs encountered at post-mortem, and the dogs above 3 year old (51.9%) were the most affected, with leptospirosis (38.5%) being the most prevalent disease diagnosed, followed by neoplasia (13.49%) while infectious canine hepatitis was the least prevalent (0.5%). The histopathological diagnosis revealed that the pneumonic patterns were that of suppurative bronchopneumonia (75%), fibrinous bronchopneumonia (10%) and interstitial pneumonia, (15%). The diseases associated with the different pneumonia seen during the histopathological examination were leptospirosis, canine distemper, and left sided heart failure. Findings has shown that pneumonia in dogs were commonly associated with leptospirosis. In the course of this study, the histopathological lesions and changes associated with the pneumonia seen in the leptospirosis cases include alveolar haemorrhages, edema, neutrophils and macrophages in the alveolar spaces and neutrophils in small pulmonary vessels. In lieu of this, pneumonia could be a major contributory factor to death associated with leptospirosis in the cases reviewed. Keywords: Archival necropcy samples, Dogs, Incidence, Leptospirosis, Pneumonia
Comparison of Preoperative Quantitative Magnetic Resonance Imaging and Clinical Assessment of Deep Pain Perception as Prognostic Tools for Early Recovery of Motor Function in Paraplegic Dogs with Intervertebral Disk Herniations

Background Prognostic tools to predict early postoperative motor function recovery (MFR) after thoracolumbar intervertebral disk herniation (IVDH) in paraplegic dogs represent an opportunity to timely implement novel therapies that could shorten recovery times and diminish permanent neurological dysfunctions. Hypothesis Fractional anisotropy (FA) values obtained using diffusion tensor imaging have a higher prognostic value than a lesion extension ratio in T2‐weighted images (T2W‐LER) and clinical assessment of deep pain perception (DPP) for MFR. Animals Thirty‐five paraplegic dogs with diagnosis of acute or subacute thoracolumbar IVDH. Methods Prospective, descriptive observational study. At admission, absence or presence of DPP, T2W‐LER, and FA values was evaluated. MFR was assessed within 4 weeks after decompressive surgery. Values of T2W‐LER and FA of dogs with and without MFR were compared using t‐tests. All 3 methods were evaluated for their sensitivity and specificity as a prognostic factor. Results No differences were found between groups regarding T2W‐LER. FA values differed statistically when measured caudally of lesion epicenter being higher in dogs without MFR compared to dogs with MFR (P = .023). Logistic regression analysis revealed significance in FA values measured caudally of the lesion epicenter (P = .033, area under the curve = 0.72). Using a cutoff value of FA = 0.660, the technique had a sensitivity of 80% and a specificity of 55%. Evaluation of DPP had a sensitivity of 73.3% and specificity of 75% (P = .007). Conclusions and Clinical Importance Evaluation of DPP showed a similar sensitivity and a better specificity predicting early MFR than quantitative magnetic resonance imaging.
A Metabolic and Body-Size Scaling Framework for Parasite Within-Host Abundance, Biomass, and Energy Flux

Energetics may provide a useful currency for studying the ecology of parasite assemblages within individual hosts. Parasite assemblages may also provide powerful models to study general principles of ecological energetics. Yet there has been little ecological research on parasite-host energetics, probably due to methodological difficulties. However, the scaling relationships of individual metabolic rate with body or cell size and temperature may permit us to tackle the energetics of parasite assemblages in hosts. This article offers the foundations and initial testing of a metabolic theory of ecology (MTE) framework for parasites in hosts. I first provide equations to estimate energetic flux through observed parasite assemblages. I then develop metabolic scaling theory for parasite abundance, energetics, and biomass in individual hosts. In contrast to previous efforts, the theory factors in both host and parasite metabolic scaling, how parasites use host space, and whether energy or space dictates carrying capacity. Empirical tests indicate that host energetic flux can set parasite carrying capacity, which decreases as predicted considering the scaling of host and parasite metabolic rates. The theory and results also highlight that the phenomenon of “energetic equivalence” is not an assumption of MTE but a possible outcome contingent on how species partition resources. Hence, applying MTE to parasites can lend mechanistic, quantitative, predictive insight into the nature of parasitism and can inform general ecological theory.
Information on Prenatal Diagnosis at the Antenatal Clinic: The Women's Experiences

The issue of information and autonomy (informed consent) in prenatal diagnosis (PND) was studied by questionnaires given to women undergoing amniocentesis (group A, n = 122) or chorionic villus biopsy (group V, n = 90). About 1/4 of the women were also interviewed. Sixty‐eight percent of the women had learnt most about PND already before visiting the antenatal clinic. More women in group A (75%) than in group V (51%) were satisfied with the information given at the clinic, probably because amniocentesis is a well‐known routine, while chorionic villus biopsy is not. Further, in both groups, more of the women who underwent PND because of age were satisfied with the information than those having PND because of strong fear of giving birth to a handicapped baby. This might be explained by the complicated character of this fear, which the medical staff could not always understand and meet. Also the women who were investigated because of a known genetic problem seemed to need more specialized genetic counselling. Fifty‐eight percent of the women were well informed about the risk of giving birth to a child with a congenital disorder and 83% about which disorders could be detected by the procedure they were undergoing. Seventy‐three percent had not felt any uneasiness when they were offered PND by their midwife or gynecologist; 18% had initiated the discussion themselves. Seventy‐four percent of the women recommended that the medical staff actively offered PND. Most women (87%) answered that the midwife's or gynecologist's attitude towards PND had been positive. The majority (85%) considered that there is a risk of persuasion in counselling. However, 75% had felt independent and autonomous in their decision‐making about PND.