Miracle-dz/UniFAR-FTUs huggingface dataset:
Profilin-I-ligand interactions influence various aspects of neuronal differentiation

Differentiating neurons extend membrane protrusions that develop into growing neurites. The driving force for neurite outgrowth is the dynamic actin cytoskeleton, which is regulated by actin-binding proteins. In this study, we describe for the first time, the role of profilin I and its ligand interactions in neuritogenesis of PC12 cells. High-level overexpression of wild-type profilin I had an inhibitory effect on neurite outgrowth. Low levels of profilin I did not disturb this process, but these cells developed many more filopodia along the neurite shafts. Low-level overexpression of mutant forms of profilin I changed one or more aspects of PC12 differentiation. Expression of a profilin I mutant that is defective in actin binding (profilin IR74E) decreased neurite length and strongly inhibited filopodia formation. Cells expressing mutants defective in binding proline-rich ligands (profilin IW3A and profilin IR136D) differentiated faster, developed more and longer neurites and more branches. The profilin IR136D mutant, which is also defective in phosphatidylinositol 4,5-bisphosphate binding, enhanced neurite outgrowth even in the absence of NGF. Parental PC12 cells treated with the ROCK inhibitor Y27632, differentiate faster and display longer neurites and more branches. Similar effects were seen in cells expressing profilin IWT, profilin IW3A and profilin IR74E. By contrast, the profilin IR136D-expressing cells were insensitive to the ROCK inhibitor, suggesting that regulation of profilin I by phosphatidylinositol 4,5-bisphosphate metabolism is crucial for proper neurite outgrowth. Taken together, our data show the importance of the interaction of profilin I with actin, proline-rich proteins and phosphatidylinositol 4,5-bisphosphate in neuronal differentiation of PC12 cells.
Anti‐interleukin‐10R1 monoclonal antibody in combination with bacillus Calmette–Guérin is protective against bladder cancer metastasis in a murine orthotopic tumour model and demonstrates systemic specific anti‐tumour immunity

Effective treatment of bladder cancer with bacillus Calmette–Guérin (BCG) depends on the induction of a T helper type (Th) 1 immune response. Interleukin (IL)‐10 down‐regulates the Th1 response and is associated with BCG failure. In this study, we investigated whether blocking IL‐10 signalling could enhance the BCG‐induced Th1 response and anti‐tumour immunity in a murine orthotopic tumour model. Treatment with BCG and anti‐IL‐10 receptor 1 monoclonal antibody (anti‐IL‐10R1 mAb) increased the interferon (IFN)‐γ to IL‐10 ratio in both splenocyte cultures and urine. Mice bearing luciferase‐expressing MB49 (MB49‐Luc) tumours were treated and followed for tumour growth by bioluminescent imaging, bladder weight and histology. Mice treated with phosphate‐buffered saline (PBS) (group 1), BCG plus control immunoglobulin (Ig)G1 (group 2) or BCG plus anti‐IL‐10R1 mAb (group 3) showed 0, 6 and 22% tumour regression, respectively. The mean bladder weight of group 3 mice was substantially lower than those of groups 1 and 2 mice. Remarkably, 36% of group 1 and 53% of group 2 mice but no group 3 mice developed lung metastasis (P = 0·02). To investigate the mechanisms underlying the effect of combination therapy, splenocytes were stimulated with S12 peptide (serine mutation at codon 12 of the K‐ras oncogene) known to be expressed in MB49‐Luc cells. Induction of ras mutation‐specific IFN‐γ and cytotoxicity was observed in mice treated with combination therapy. These observations indicate that BCG, in combination with anti‐IL‐10R1 mAb, induces enhanced anti‐tumour immunity that is protective against lung metastasis. Anti‐IL‐10R1 mAb demonstrates systemic effects and may prove useful in clinical practice for treating bladder cancer in high‐risk patients.
Assessment of Attitude of Education Students towards Teaching -practice in Nasarawa State University Keffi, Nigeria

The paper is a descriptive survey which sought to assess the attitude of student-teachers towards teaching practice. This study was conducted in the Faculty of Education, Nasarawa state University Keffi. The sample comprised of 150 students who were on teaching practice in 20010/2011 Session, 20 principals and 40 teachers of practicing schools were drawn by simple balloting. Four research questions and one hypothesis guided the study. Two instruments (TPQ and TPAQPT were developed by the researchers and used for data collection. The instruments were validated and an internal consistency of 0.80 and 0.76 respectively were calculated using Cronbach Alpha techniques. Data was analyzed using means and z-Test statistics. The findings revealed that (i) students show a positive attitude towards teaching practice. (ii) female students showed more positive attitude to teaching practice than male students. Also, problems militating against effective teaching practice were determined. It was concluded that if all the strategies for improving students attitude towards teaching practice would be adopted the students’ attitude towards teaching practice, no doubt, will be affected positively. Based on the findings, recommendations were made among others that the Faculty should have a micro-teaching laboratory where the students will be well exposed to the rudiments of teaching before they should be sent to schools for actual practice. Keywords: Attitude, Teaching practice, Micro-teaching laboratory, Actual practice
Evaluation of Non-animal Methods for Assessing Skin Sensitisation Hazard: A Bayesian Value-of-Information Analysis

This paper offers a Bayesian Value-of-Information (VOI) analysis for guiding the development of non-animal testing strategies, balancing information gains from testing with the expected social gains and costs from the adoption of regulatory decisions. Testing is assumed to have value, if, and only if, the information revealed from testing triggers a welfare-improving decision on the use (or non-use) of a substance. As an illustration, our VOI model is applied to a set of five individual non-animal prediction methods used for skin sensitisation hazard assessment, seven battery combinations of these methods, and 236 sequential 2-test and 3-test strategies. Their expected values are quantified and compared to the expected value of the local lymph node assay (LLNA) as the animal method. We find that battery and sequential combinations of non-animal prediction methods reveal a significantly higher expected value than the LLNA. This holds for the entire range of prior beliefs. Furthermore, our results illustrate that the testing strategy with the highest expected value does not necessarily have to follow the order of key events in the sensitisation adverse outcome pathway (AOP).
Heparin induces dimerization and confers proliferative activity onto the hepatocyte growth factor antagonists NK1 and NK2

Hepatocyte growth factor (HGF) is a potent epithelial mitogen whose actions are mediated through its receptor, the proto-oncogene c-Met. Two truncated variants of HGF known as NK1 and NK2 have been reported to be competitive inhibitors of HGF binding to c-Met, and to function as HGF antagonists (Lokker, N.A., and P.J. Godowski. 1993. J. Biol. Chem. 268: 17145-17150; Chan, A.M., J.S. Rubin, D.P. Bottaro, D.W. Hirschfield, M. Chedid, and S.A. Aaronson. 1991. Science (Wash. DC). 254:1382-1387). We show here, however, that NK1 acts as a partial agonist in mink lung cells. Interestingly, NK1, which is an HGF antagonist in hepatocytes in normal conditions, was converted to a partial agonist by adding heparin to the culture medium. The interaction of NK1 and heparin was further studied in BaF3 cells, which express little or no cell surface heparan sulfate proteoglycans. In BaF3 cells transfected with a plasmid encoding human c-Met, heparin and NK1 synergized to stimulate DNA synthesis and cell proliferation. There was no effect of heparin on the IL-3 sensitivity of BaF3-hMet cells, and no effect of NK1 plus heparin in control BaF3 cells, indicating that the response was specific and mediated through c-Met. The naturally occurring HGF splice variant NK2 also stimulated DNA synthesis in mink lung cells and exerted a heparin-dependent effect on BaF3-hMet cells, but not on BaF3-neo cells. The activating effect of heparin was mimicked by a variety of sulfated glycosaminoglycans. Mechanistic studies revealed that heparin increased the binding of NK1 to BaF3-hMet cells, stabilized NK1, and induced dimerization of NK1. Based on these studies, we propose that the normal agonist activity of NK1 and NK2 in mink lung cells is due to an activating interaction with an endogenous glycosaminoglycan. Consistent with that model, a large portion of the NK1 binding to mink lung cells could be blocked by heparin. Moreover, a preparation of glycosaminoglycans from the surface of mink lung cells induced dimerization of NK1. These data show that the activity of NK1 and NK2 can be modulated by heparin and other related glycosaminoglycans to induce proliferation in cells expressing c-Met.
Perbedaan peningkatan kemampuan pemecahan masalah dan komunikasi matematik siswa pada pembelajaran berbasis masalah dan pembelajaran langsung pada siswa Sekolah Menengah Pertama

Tujuan penelitian dalam desain Eksperimen semu ini menyelidiki perbedaan peningkatan Pendekatan (PBM) atas kemampuan pemecahan masalah matematik siswa dan kemampuan komunikasi matematika siswa. Penelitian ini dilaksanakan di SMPN 29 Medan sebanyak 74 siswa. Penelitian ini merupakan suatu studi eksperimen dengan desain penelitian pre-test-post-test control group design . Populasi dalam penelitian ini adalah seluruh siswa kelas 1 (satu) dengan mengambil sampel dua kelas (kelas eksperimen dan kelas kontrol) melalui teknik random sampling. Data dianalisis dengan uji ANAVA dua jalur. Sebelum digunakan uji ANAVA dua jalur terlebih dahulu dilakukan uji homogenitas dalam penelitian dan normalitas dalam penelitian ini dengan taraf signifikan 5%. Hasil analisis data menunjukkan bahwa rata-rata N-Gain tes kemampuan pemecahan masalah kelas eksperimen adalah 0,73 dan kelas kontrol adalah 0,49 dengan nilai sig = 0, dengan 0 < α = 0,05 maka terdapat perbedaan peningkatan kemampuan pemecahan masalah matematik siswa yang diajarkan dengan Pendekatan (PBM) dengan Pembelajaran Langsung. Rata-rata N-Gain tes kemampuan komunikasi eksperimen dan kontrol adalah 0,64 dan 0,44 dengan -value (2-tailed) adalah 0, dengan 0 < α = 0,05 maka terdapat perbedaan peningkatan kemampuan komunikasi matematik siswa yang diajarkan dengan Pendekatan (PBM) dan Pembelajaran Langsung.
EXPERIMENTAL DEMONSTRATION OF A CAUSAL RELATIONSHIP BETWEEN HETEROGENEITY OF SELECTION AND GENETIC DIFFERENTIATION IN QUANTITATIVE TRAITS

Abstract Comparisons of estimates of genetic differentiation at molecular markers (FST) and at quantitative traits (QST) are a means of inferring the level and heterogeneity of selection in natural populations. However, such comparisons are questionable because they require that the influence of drift and selection on QST be detectable over possible background influences of environmental or nonadditive genetic effects on QST-values. Here we test this using an experimental evolution approach in metapopulations of Arabidopsis thaliana experiencing different levels of drift and selection heterogeneity. We estimated the intensity and heterogeneity of selection on morphological and phenological traits via selection differentials. We demonstrate that QST-values increased with increasing selection heterogeneity when genetic drift was limited. The effect of selection on QST was thus detectable despite significant genotype-by-environment interactions that most probably biased the estimates of genetic differentiation. Although they cannot be used as a direct validation of the conclusions of prior studies, our results strongly support both the relevance of QST as an estimator of genetic differentiation and the role of local selection in shaping the genetic differentiation of natural populations.
Performance Analysis of an Hybrid ARQ Adaptation of NDMA Schemes

Traditionally, an unsuccessful packet reception, either due to channel conditions or interference from multiple sources (collisions), is discarded and followed by a new reception of the same packet. Network diversity multiple access (NDMA) handles collisions by using time diversity multipacket reception: the base station forces terminals to transmit P copies of each packet when P terminals collide. This rigidity of having P copies is unsuitable for poor propagation conditions. It might be desirable to have more than P copies of a given packet, adopting a technique for single packet reception called hybrid-ARQ. Hybrid-ARQ effectively decreases the packet error rate. This paper proposes such mechanism for multi-packet reception, called hybrid-ARQ NDMA (H-NDMA), a gated slotted random access protocol, where the access mechanism forces the terminals involved in a collision with reception errors to transmit more than P times. Analytical models for the throughput, delay and energy per useful packet are proposed. The energy performance is analyzed for bounded average delay constraints. The proposed system's performance is evaluated for a single-carrier with frequency domain equalization (SC-FDE) scheme, and compared with the classical NDMA protocol. The comparison highlights that H- NDMA is scalable for an increasing number of terminals and network load.
Highly Nonlinear Birefringent Soft Glass Photonic Crystal Fiber With Liquid Crystal Core

In this letter, highly nonlinear birefringent soft glass photonic crystal fiber is presented and analyzed using the full vectorial finite-difference method. The suggested design has a central hole infiltrated with a nematic liquid crystal (NLC). The reported design offers high birefringence of 0.042 at the operating wavelength 1.55 μm with low losses for the two polarized modes. In addition, high nonlinearities of 425.5 W-1 km-1 and 470.8 W-1 km-1 are achieved for the quasi-transverse electric (TE) and quasi-transverse magnetic (TM) modes, respectively, at the operating wavelength of 1.55 μm.
Cervical cancer screening in adolescents: an evidence-based internet education program for practice improvement among advanced practice nurses.

BACKGROUND The literature reports great variation in the knowledge levels and application of the recent changes of cervical cancer screening guidelines into clinical practice. Evidence-based screening guidelines for the prevention and early detection of cervical cancer offers healthcare providers the opportunity to improve practice patterns among female adolescents by decreasing psychological distress as well as reducing healthcare costs and morbidities associated with over-screening. PURPOSE The purpose of this pilot intervention study was to determine the effects of a Web-based continuing education unit (CEU) program on advanced practice nurses' (APNs) knowledge of current cervical cancer screening evidence-based recommendations and their application in practice. This paper presents a process improvement project as an example of a way to disseminate updated evidence-based practice guidelines among busy healthcare providers. METHODS This Web-based CEU program was developed, piloted, and evaluated specifically for APNs. The program addressed their knowledge level of cervical cancer and its relationship with high-risk human papillomavirus. It also addressed the new cervical cancer screening guidelines and the application of those guidelines into clinical practice. FINDINGS Results of the study indicated that knowledge gaps exist among APNs about cervical cancer screening in adolescents. However, when provided with a CEU educational intervention, APNs' knowledge levels increased and their self-reported clinical practice behaviors changed in accordance with the new cervical cancer screening guidelines. LINKING EVIDENCE TO PRACTICE Providing convenient and readily accessible up-to-date electronic content that provides CEU enhances the adoption of clinical practice guidelines, thereby decreasing the potential of the morbidities associated with over-screening for cervical cancer in adolescents and young women.
Fission of collapsing cavitation bubbles

High-speed observations clearly show that though a collapsing cavitation bubble approaches its minimum size as a coherent single volume, it usually reappears in the first rebounding frame as a cloud of much smaller bubbles or as a highly distorted single volume. This paper explores two mechanisms that may be responsible for that bubble fission process, one invoking a Rayleigh–Taylor stability analysis and the other using the so-called microjet mechanism. Both approaches are shown to lead to qualitatively similar values for the number of fission fragments and the paper investigates the flow parameters that effect that number. Finally, the additional damping of the Rayleigh–Plesset single-bubble calculation caused by the fission process is investigated; it is shown that the fission damping dominates other contributions normally considered and is consistent with the number of collapses and rebounds that are observed to occur in experiments.
The relationships between goal orientation, perfectionism, parental involvement, peer climate, enjoyment, and intention to continue in sport in children.

This investigation examined the relationships between parental involvement, peer-initiated climates, and perfectionism to goal orientation as well as children's enjoyment and the intention to continue playing sport in youth sport. Participants were 188 athletes, 100 boys (M = 12.06, SD = 1.06) and 88 girls (M = 12.18, SD = .73). The athletes completed the TEOSQ, Sport MPS, PIAS, and the PeerMCYSQ. Parental support and peer task environment was related to girls' and boy's task orientation. For boys, personal standards, parental pressure, and fewer concerns over mistakes, also were related to task orientation. Ego orientation was related to peer-initiated ego and task climates, for the boys. For the girls, higher personal standard was the only variable related to ego orientation. For enjoyment, task orientation was the strongest predictor for the girls and the only predictor for the boys for enjoyment. The fewer concerns girls had over mistakes the more enjoyment they reported. For girls and boys, intention to continue playing next season was predicted only by enjoyment. However, results were varied when intention to play next year was examined. For boys, no predictors were discovered whereas for girls, higher levels of enjoyment and task orientation, and lower levels of parental support and pressure related to intention.
Upper bounds on the number of hidden neurons in feedforward networks with arbitrary bounded nonlinear activation functions

It is well known that standard single-hidden layer feedforward networks (SLFNs) with at most N hidden neurons (including biases) can learn N distinct samples (x(i),t(i)) with zero error, and the weights connecting the input neurons and the hidden neurons can be chosen "almost" arbitrarily. However, these results have been obtained for the case when the activation function for the hidden neurons is the signum function. This paper rigorously proves that standard single-hidden layer feedforward networks (SLFNs) with at most N hidden neurons and with any bounded nonlinear activation function which has a limit at one infinity can learn N distinct samples (x(i),t(i)) with zero error. The previous method of arbitrarily choosing weights is not feasible for any SLFN. The proof of our result is constructive and thus gives a method to directly find the weights of the standard SLFNs with any such bounded nonlinear activation function as opposed to iterative training algorithms in the literature.
The transcription factor NFATc2 controls IL-6–dependent T cell activation in experimental colitis

The nuclear factor of activated T cells (NFAT) family of transcription factors controls calcium signaling in T lymphocytes. In this study, we have identified a crucial regulatory role of the transcription factor NFATc2 in T cell–dependent experimental colitis. Similar to ulcerative colitis in humans, the expression of NFATc2 was up-regulated in oxazolone-induced chronic intestinal inflammation. Furthermore, NFATc2 deficiency suppressed colitis induced by oxazolone administration. This finding was associated with enhanced T cell apoptosis in the lamina propria and strikingly reduced production of IL-6, -13, and -17 by mucosal T lymphocytes. Further studies using knockout mice showed that IL-6, rather than IL-23 and -17, are essential for oxazolone colitis induction. Administration of hyper-IL-6 blocked the protective effects of NFATc2 deficiency in experimental colitis, suggesting that IL-6 signal transduction plays a major pathogenic role in vivo. Finally, adoptive transfer of IL-6 and wild-type T cells demonstrated that oxazolone colitis is critically dependent on IL-6 production by T cells. Collectively, these results define a unique regulatory role for NFATc2 in colitis by controlling mucosal T cell activation in an IL-6–dependent manner. NFATc2 in T cells thus emerges as a potentially new therapeutic target for inflammatory bowel diseases.
Twenty Cases Suggestive of Reincarnation

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Mobility-Prediction-Aware Bandwidth Reservation Scheme for Mobile Networks

Bandwidth is an extremely valuable and scarce resource in mobile networks; therefore, efficient mobility-aware bandwidth reservation is necessary to support multimedia applications (e.g., video streaming) that require quality of service (QoS). In this paper, we propose a distributed bandwidth reservation scheme called the mobility-prediction-aware bandwidth reservation (MPBR) scheme. The objective of MPBR is to reduce handoff call dropping rate and maintain acceptable new call blocking rate while providing efficient bandwidth utilization. MPBR consists of a handoff time estimation (HTE) scheme that aims to estimate the time windows when a user will perform handoffs along the path to his destination, an available bandwidth estimation (ABE) scheme that aims to estimate in advance available bandwidth, during the computed time windows in the cells to be traversed by the user to his destination, and an efficient call admission control (ECaC) scheme that aims to control bandwidth allocation in the network cells. The simulation results show that MPBR outperforms existing schemes in terms of reducing handoff call dropping rate.