Miracle-dz/UniFAR-FTUs huggingface dataset:
The effects of recorded sedative music on the physiology and behaviour of premature infants with a respiratory disorder

This study tested the safety of using recorded sedative music (RSM) with 34 week old premature infants with a respiratory disorder. Data was collected to measure the effect of RSM on the physiological stability and behaviours of these infants. Twenty two infants were randomly assigned to one of two groups, the music group or control group. The music group received 20 minutes of recorded music per day for 4 days. The control group received no music for 4 days. Each infant was observed for a total of 45 minutes each day for the 4 days that they participated The data collectors were unaware of group allocation and listened to masking music when conducting observations. Data measures were heart rate, respiration rate, oxygen saturation, and infant behaviours. The use of RSM had no negative effect on the physiology or behaviour of the infants in the music group. There were no significant differences between the groups with regard to heart rate, respiration rate, or oxygen saturation. Behavioural results also showed no significant differences between the behavioural states of the two groups. The lack of significant results is attributed to a number of factors including a potentially skewed sample and insufficient exposure to the music to establish an effect. (author abstract)
A New Leanchoiliid Megacheiran Arthropod from the Lower Cambrian Emu Bay Shale, South Australia

The Leanchoiliidae is well-known from abundant material of Leanchoilia, from the Burgess Shale and Chengjiang Konservat-Lagerstätten. The first Australian member of the group is Oestokerkus megacholix gen. et sp. nov., described from the Emu Bay Shale (Cambrian Series 2, Stage 4), at Buck Quarry, Kangaroo Island, South Australia, and is intermediate in age between the well known leanchoiliid species from the Burgess Shale and Chengjiang. Phylogenetic analysis of “short great appendage” arthropods (Megacheira) in the context of the chelicerate stem group resolves the Australian species as sister to Burgess Shale, Utah, and Chengjiang Leanchoilia species, but most readily distinguished from Leanchoilia and Alalcomenaeus by a different telson shape, interpreted as being forked, widening distally, and with a few dorsally curved spines at the posterior angle. Leanchoiliid interrelationships are stable to alternative character weights, and Megacheira corresponds to a clade in most analyses.
Genetics of Host-Controlled Restriction and Modification of Deoxyribonucleic Acid in Escherichia coli

Lederberg, Seymour (Brown University, Providence, R.I.). Genetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli. J. Bacteriol. 91:1029–1036. 1966. —The locus for the host specific restriction and modification of deoxyribonucleic acid in Escherichia coli has been mapped by matings between mutants for these characters in strains K-12, C600, and B. Linkage analysis and kinetics of marker transfer indicate that a single or closely linked multiple chromosomal site located about 4 min counterclockwise to leucine is responsible for these activities. Secondary factors which affect the quantitative level of restriction also were detected. Wild-type recombinants were isolated in crosses between rm− (restriction or modification, or both) mutants. The expression in zygotes of the restrictionless character of a rm− donor is masked by a separate, physiological impairment of restriction, which results from mating and is independent of the modification state of the donor. The relevance of the restriction character to mating incompatibilities in these and other bacterial strains is considered.
Microbial contamination of open‐but‐unused portions of wound dressings stored in home settings

The purpose of this study was to investigate incidence rates and levels of microbial contamination in open‐but‐unused portions of wound dressings stored in home settings. Portions of wound dressings were collected at up to four home visits for 104 clients undertaking wound management within their home. A control sample and stored sample was collected on each home visit and sent for pathology testing to identify levels of microbial contamination. The stored open‐but‐unused wound dressings were managed according to a written protocol. Of the tested samples (n = 776), 6% of control samples and 7% of test samples had microbial contamination. From regression analysis, the stored samples were more likely to have microbial contamination than control samples, but results were not statistically significant. In comparing occasions of storage and handling across four home visits, after adjusting for sample group and dressing type, none of the home visit occasion regressions were statistically significant. In conclusion, storage of open‐but‐unused portions of wound dressings kept in home settings does not appear to increase the rate of microbial contamination compared to newly opened wound dressings.
Expansion of Human Adult Stem Cells from Bone Marrow Stroma: Conditions that Maximize the Yields of Early Progenitors and Evaluate Their Quality

There is considerable interest in the biology and therapeutic potential of adult stem cells from bone marrow stroma, variously referred to as mesenchymal stem cells or marrow stromal cells (MSCs). Human MSCs can expand rapidly in culture, but the rate of expansion and the yields of multipotential progenitors are inversely related to the plating density and incubation time of each passage. We have defined conditions for optimizing the yields of cultures enriched for early progenitors. Also, we developed a simple method for assessing the quality of the cultures by phase‐contrast microscopy and image analysis or by forward light scatter in a flow cytometer. The cells expanded most rapidly on day 4 after plating, with a minimum average doubling time of about 10 hours for cells initially plated at 10 or 50 cells/cm2. After plating the cells at 1 to 1,000 cells/cm2, the cultures underwent a time‐dependent transition from early progenitors, defined as thin, spindle‐shaped cells (RS‐1A), to wider, spindle‐shaped cells (RS‐1B), and to still wider, spindle‐shaped cells (RS‐1C). Assays for adipogenesis demonstrated that the adipogenic potential of cultures was directly related to their ability to generate single‐cell‐derived colonies and their enrichment for RS‐1A cells. In contrast, cultures enriched for RS‐1B cells showed the greatest potential to differentiate into cartilage in a serum‐free system. The results indicate that, when preparing cultures of human MSCs, it is necessary to compromise between conditions that provide the highest overall yields and those that provide the highest content of early progenitor cells.
The effects of cognitive abilities on driving in people with Parkinson's disease

Objective: The aim was to develop a cognitive screening procedure, which could be used to identify cognitive problems in patients with Parkinson's disease, which might affect their safety to drive. Design: Two group comparison of those found safe to drive and those found unsafe. Setting: People living in the community who were attending an outpatient Movement Disorders clinic or who had been referred to a Regional Mobility Centre. Participants: Fifty-one people with Parkinson's disease who were driving or who wished to resume driving. The 41 men and 10 women were aged 44 – 85 years (mean 64.4 SD 9.1). Main measures: Webster's Rating Scale, Unified Parkinson's Disease Rating Scale motor examination, Stroke Drivers Screening Assessment, Adult Memory and Information Processing Battery, Stroop, Paced Auditory Serial Addition Task and a Tapping task. Results: The unsafe drivers were significantly more disabled, as assessed on Webster's Scale, than those who were found safe to drive. There were no significant differences in the cognitive abilities of safe and unsafe drivers. The most common faults, which caused drivers to be judged unsafe, were lack of observations to the side at junctions, poor positioning on the road and poor driving on roundabouts. There were significant correlations (p < 0.05) between driving ability and performance on the SDSA Dot Cancellation task and the AMIPB Story Recall and Information Processing A. Conclusions: Cognitive abilities were not found to be associated with fitness to drive in people with Parkinson's disease. Webster's Rating Scale differentiated between safe and unsafe drivers. This could be used to determine who to refer to a mobility centre for advice on fitness to drive.
Degradative enzymes of oral streptococci.

Members of the Streptococcus sanguis group (SSG) and Streptococcus milleri group (SMG) were screened for their ability to produce glycosidase, arylamidase (peptidase), protease, dextranase and glycosyltransferase activities. Species within each group produced unique patterns of activity. The most commonly produced glycosidases were beta-D-glucosidase, beta-D-galactosidase, N-acetyl-beta-D-glucosaminidase and N-acetyl-beta-D-galactosaminidase and the least commonly produced glycosidase activity was beta-fucosidase with Streptococcus intermedius (SMG) being the only species capable of producing the activity. For arylamidase activity, the most commonly produced type was lysine-arylamidase. Glycosidase and arylamidase activities were localized to particular sub-cellular fractions. alpha-galactosidase was found only in culture supernatant fluids whereas N-acetyl-beta-D-glucosaminidase was found in all fractions; the culture supernatant, cell wall, cell membrane and cytoplasm. No arylamidase activity was seen in culture supernatants. Phe-arg-arylamidase was found only in cytoplasmic fractions whereas val-pro-argarylamidase was found in cell walls, cell membranes and cytoplasmic fraction. Protease activity was measured as the degradation of bovine serum albumin (BSA) and casein. Casein was degraded by a number of strains whereas no species/strains were able to degrade BSA. Streptococcus intermedius, Streptococcus constellatus (SMG), Streptococcus mitior and Streptococcus defectivus (SSG) were the only species that produced hyaluronidase and no species produced chondroitin sulphatase. The groups were also examined for their abilities to produce glycosyltransferase and dextranase. Strep. sanguis, Streptococcus gordonii, Streptococcus mitis and Streptococcus oralis produced glucosyltransferase and, with the exception of the latter species, fructosyltransferase. No species within the SMG was capable of producing either glycosyltransferase. (ABSTRACT TRUNCATED AT 250 WORDS)
Going offline: Personality and its effects on the transition of online relationships to the offline world

Because the internet is a relatively new technology, few studies have researched the correlations between online behavior and personality. As such, this study set out to determine if online friendships and personality are related. In this study differences in personality were examined between individuals who have met their online friends face-to-face and those who have not. A survey was distributed online using the Five Factor Model to measure personality. Participants in this study were recruited from an online community called Nerdfighteria. The results found that the facets of the Five Factor Model of personality were largely found not to be predictors of whether or not individuals will meet their online friends in an offline setting. Of the five personality factors measured, extraversion was the only one that had statistically significant differences in mean scores between individuals who chose to meet their online friends offline and those chose not to. Individuals with higher scores of extraversion are more likely to meet their online friends face-to-face than those with lower scores of extraversion. Because the difference in mean scores of these two groups was so small, it is possible extraversion does not truly have an effect. As such, it is difficult to determine if personality truly has a relationship to this online behavior.
Paradoxical tuberculosis‐associated immune reconstitution inflammatory syndrome in children

Paradoxical tuberculosis (TB)‐associated Immune Reconstitution Inflammatory Syndrome (IRIS) is a common complication of combination antiretroviral treatment (cART) initiation in adults residing in resource‐limited regions. Little is known about the burden and presentation of TB‐IRIS in children initiating cART while receiving TB treatment.
Aminoglycoside binding to the HIV-1 RNA dimerization initiation site: thermodynamics and effect on the kissing-loop to duplex conversion

Owing to a striking, and most likely fortuitous, structural and sequence similarity with the bacterial 16 S ribosomal A site, the RNA kissing-loop complex formed by the HIV-1 genomic RNA dimerization initiation site (DIS) specifically binds 4,5-disubstituted 2-deoxystreptamine (2-DOS) aminoglycoside antibiotics. We used chemical probing, molecular modeling, isothermal titration calorimetry (ITC) and UV melting to investigate aminoglycoside binding to the DIS loop–loop complex. We showed that apramycin, an aminoglycoside containing a bicyclic moiety, also binds the DIS, but in a different way than 4,5-disubstituted 2-DOS aminoglycosides. The determination of thermodynamic parameters for various aminoglycosides revealed the role of the different rings in the drug–RNA interaction. Surprisingly, we found that the affinity of lividomycin and neomycin for the DIS (Kd ∼ 30 nM) is significantly higher than that obtained in the same experimental conditions for their natural target, the bacterial A site (Kd ∼ 1.6 µM). In good agreement with their respective affinity, aminoglycoside increase the melting temperature of the loop–loop interaction and also block the conversion from kissing-loop complex to extended duplex. Taken together, our data might be useful for selecting new molecules with improved specificity and affinity toward the HIV-1 DIS RNA.
Knockdown of mitogen‐activated protein kinase (MAPK) signalling in the midgut of Anopheles stephensi mosquitoes using antisense morpholinos

Arthropod‐borne infectious diseases are responsible for nearly 1.5 million deaths annually across the globe, with malaria responsible for >50% of these deaths. Recent efforts to enhance malaria control have focused on developing genetically modified Anopheles mosquitoes that are resistant to malaria parasite infection by manipulating proteins that are essential to the immune response. Although this approach has shown promise, the lack of efficient genetic tools in the mosquito makes it difficult to investigate innate immunity using reverse genetics. Current gene knockdown strategies based on small interfering RNA are typically labourious, inefficient, and require extensive training. In the present study, we describe the use of morpholino antisense oligomers to knockdown MEK‐ERK signalling in the midgut of Anopheles stephensi through a simple feeding protocol. Anti‐MEK morpholino provided in a saline meal was readily ingested by female mosquitoes with minimal toxicity and resulted in knockdown of total MEK protein levels 3–4 days after morpholino feeding. Further, anti‐MEK morpholino feeding attenuated inducible phosphorylation of the downstream kinase ERK and, as predicted by previous work, reduced parasite burden in mosquitoes infected with Plasmodium falciparum. To our knowledge, this is the first example of morpholino use for target protein knockdown via feeding in an insect vector. Our results suggest this method is not only efficient for studies of individual proteins, but also for studies of phenotypic control by complex cell signalling networks. As such, our protocol is an effective alternative to current methods for gene knockdown in arthropods.
Glucose, Insulin and Somatostatin Infusion for the Determination of Insulin Resistance in Liver Cirrhosis

Twelve patients with liver cirrhosis and ten normal subjects were studied. Using a constant intravneous infusion of glucose, insulin and somatostatin over 2 1/2 hours we determined the stteady state plasma glucose level (SSPG) in order to measure insulin resistance. The results demonstrated that the cirrhotic patients were insulin resistant compared to normals and that plasma glucagon does not account for the insulin resistance in these patients.
Cartographies of Stalin: Place, Scale, and Reputational Politics

In this article we explore the spatial variation of support for former Soviet leader Joseph Stalin in his home country, Georgia. This research contributes to the literature on reputational politics by highlighting the role of spatial, rather than only social, factors in the construction of public opinion about Stalin. We illustrate how geographic factors impact Georgians' perceptions of the Soviet dictator by examining various aspects of place at various scales—including distance to Gori, Stalin's birthplace, the history of urbanization, and economic and political indices. To this end we map attitudes toward the Soviet dictator and use a multilevel spatial regression technique to explain variance in support for Stalin across forty-seven districts of Georgia. This enables us to explore the impact of place on people's attitudes by examining (1) whether there are local and regional hotspots of Stalin admiration and, if so, (2) what the contextual explanations of these attitudes are.
Cognitive-Enhancing Effects of Polygalasaponin Hydrolysate in Aβ 25–35-Induced Amnesic Mice

Polygalasaponins are the major active constituents of Polygala tenuifolia exhibiting antiamnesic activity, but their applications are limited due to their toxicities. Evidence showed that the toxicities can be attenuated by hydrolysis. Herein, effects of a hydrolysate of polygalasaponins (HPS) on cognitive impairment induced by Aβ 25−35 were assessed by Morris water maze and step-through passive avoidance tests. The impaired spatial reference memory was improved by HPS (50 and 100 mg/kg). In the acquisition trial of step-through test, HPS (50 and 100 mg/kg) increased the latency into the dark chamber and decreased the error frequency significantly (P < .05). However, no significant change was observed during the retention trial. Additionally, HPS increased the corresponding SOD activities (62.34%, 22.09%) and decreased MDA levels (28.21%, 32.35%) in both cortex and hippocampus as compared to model animals. These results show that HPS may be a useful treatment against amnesia probably via its antioxidant properties.
Least Squares Temporal Difference Learning and Galerkin ’ s Method

The problem of estimating the value function underlying a Markovian reward process is considered. As it is well known, the value function underlying a Markovian reward process satisfied a linear fixed point equation. One approach to learning the value function from finite data is to find a good approximation to the value function in a given (linear) subspace of the space of value functions. We review some of the issues that arise when following this approach, as well as some results that characterize the finite-sample performance of some of the algorithms. 1 Markovian Reward Processes Let X be a measurable space and consider a stochastic process (X0, R1, X1, R2, X2, . . .), where Xt ∈ X and Rt+1 ∈ R, t = 0, 1, 2, . The process is called a Markovian Reward process if • (X0, X1, . . .) is a Markov process, and • for any t ≥ 0, given Xt, Xt+1 the distribution of Rt+1 is independent of the history of the process. Here, Xt is called the state of the system at time t, while Rt+1 is the reward associated to transitioning from Xt to Xt+1. We shall denote by P the Markovian kernel underlying the process: Thus, the distribution of (Xt+1, Rt+1) given Xt is given by P(·, ·|Xt), t = 0, 1, . Fix the so-called discount factor 0 ≤ γ ≤ 1 and define the (total discounted) return associated to the process R = ∞ ∑
Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potential.

Primitive and definitive erythropoiesis represent distinct hematopoietic programs that differ with respect to stage of development, transcriptional control, and growth regulation. Although these differences have been recognized for some time, the relationship of the two erythroid lineages to each other is not well established. We have used a model system based on the hematopoietic development of embryonic stem (ES) cells in culture to investigate the origins of the earliest hematopoietic populations. Using ES cells transduced with a retrovirus that overexpresses the HOX11 gene, we have established factor-dependent hematopoietic cell lines that represent novel stages of embryonic hematopoiesis. Analysis of three of these cell lines indicates that they differ with respect to cytokine responsiveness, cell surface markers, and developmental potential. Two of the cell lines, EBHX1 and EBHX11, display the unique capacity to generate both primitive and definitive erythroid progeny as defined by morphology and expression of betaH1 and betamajor globin. The third line, EBHX14, has definitive erythroid and myeloid potential, but is unable to generate cells of the primitive erythroid lineage. Analysis of the cytokine responsiveness of the two lines with primitive erythroid potential has indicated that exposure to leukemia inhibitory factor (LIF) results in the upregulation of betaH1 and a change in cellular morphology to that of primitive erythrocytes. These findings are the first demonstration of a clonal cell line with primitive and definitive hematopoietic potential and support the interpretation that these lineages may arise from a common precursor in embryonic life. In addition, they suggest that LIF could play a role in the regulation of primitive erythropoiesis.
Dynamics of the components of energy intake between Spanish and Mexican preschool children: energy density and food volume in two contexts.

OBJECTIVE To determine the differences in the dynamics of dietary energy density (ED), food volume (FV) and energy intake (EI) between two groups of healthy children, in normal conditions, from Spain and Mexico. METHODS Crossectional study which analyses the habitual diet of two healthy children groups, 1-4 years old, from Reus (Spain, n = 203) and Guadalajara (Mexico, n = 147). Dietary intake was assessed using the 24-hour recall. Anthropometric data were also obtained. We estimated Z-score of weight, height and BMI, and EI (kcal/day), ED (kcal/g), FV (g/day), El/kg body weight (kcal/kg/day) and FV/kg body weight (g/kg/day). RESULTS The Spanish children consumed significantly more cereals (p < 0.05), vegetables, meat, fish and eggs than the Mexican children (p < 0.001), while the latter consumed significantly more sweets (p < 0.001). The mean El/kg body weight was 107.7 ± 36.2 kcal/kg/day in the children from Reus, and 102.4 ± 38.8 kcal/kg/day in the children from Guadalajara, without significant differences. While the ED was significantly higher (p < 0.001) in the Spanish sample (1.41 ± 0.35 kcal/g) that in the Mexican one (1.19 ± 0.37 kcal/g), we observed the contrary on FV per kilogram of weight: it was significantly (p < 0.001) greater in Mexicans (91.0 ± 36.1 g/kg/day) than in Spanish (79.5 ± 27.5 g/kg/day). CONCLUSION In two populations with different contexts, the balance between energy intake and energy requirements is achieved in different ways, allowing energy intake per unit of weight and growth to be adequate. Future studies are needed to clarify the factors of a possible alteration of this equilibrium through time, in such a way, that it would probably contribute to the development of overweight and obesity in several environments.