Miracle-dz/UniFAR-FTUs huggingface dataset:
Construyendo Capital Social: cómo las noticias y la fuerza de los vínculos en la discusión política fomentan la reciprocidad

Reciprocity, a social principle that emerges in non-negotiated, inter-personal exchange, is arguably a key construct in generating social capital. However, little research has made this empirical connection. Building on an Orientation-Stimulus-Reasoning-Orientation-Response model, this study advances a theoretical and empirical model in which news use and political discussion tie strength contributes to the development of reciprocity and social capital. Drawing on two-wave panel data, results suggest that reciprocity is a strong predictor of social capital. News media use also directly predicts reciprocity, as well as political discussion with both strong and weak ties, and social capital. Additionally, discussion with weak ties and reciprocity mediate the relationship between news use and social capital.
Expression of Antizyme Inhibitor 2 in Mast Cells and Role of Polyamines as Selective Regulators of Serotonin Secretion

Background Upon IgE-mediated activation, mast cells (MC) exocytose their cytoplasmic secretory granules and release a variety of bioactive substances that trigger inflammatory responses. Polyamines mediate numerous cellular and physiological functions. We report here that MCs express antizyme inhibitor 2 (AZIN2), an activator of polyamine biosynthesis, previously reported to be exclusively expressed in the brain and testis. We have investigated the intracellular localization of AZIN2 both in resting and activated MCs. In addition, we have examined the functional role of polyamines, downstream effectors of AZIN2, as potential regulators of MC activity. Methodology/Principal Findings Immunostainings show that AZIN2 is expressed in primary and neoplastic human and rodent MCs. We demonstrate that AZIN2 localizes in the Vamp-8 positive, serotonin-containing subset of MC granules, but not in tryptase-containing granules, as revealed by double immunofluorescence stainings. Furthermore, activation of MCs induces rapid upregulation of AZIN2 expression and its redistribution, suggesting a role for AZIN2 in secretory granule exocytosis. We also demonstrate that release of serotonin from activated MCs is polyamine-dependent whereas release of histamine and β-hexosaminidase is not, indicating a granule subtype-specific function for polyamines. Conclusions/Significance The study reports for the first time the expression of AZIN2 outside the brain and testis, and demonstrates the intracellular localization of endogenous AZIN2 in MCs. The granule subtype-specific expression and its induction after MC activation suggest a role for AZIN2 as a local, in situ regulator of polyamine biosynthesis in association with serotonin-containing granules of MCs. Furthermore, our data indicates a novel function for polyamines as selective regulators of serotonin release from MCs.
Density graphs and separators

We propose a class of graphs that would occur naturally in finite-element problems, and we prove a bound on separators for this class of graphs. For three-dimensional graphs, our separator bound is $O(N^{2/3})$. We also propose a simple randomized algorithm to find this separator in $O(N)$ time. Such an algorithm would be used as a preprocessing step for the domain decomposition method of efficiently solving a finite-element problem on a parallel computer. This paper generalizes ``local graphs'''' of Vavasis [1990] to the case of graphs with varying densities of nodes. It also generalizes aspects of Miller and Thurston''s [1990] ``stable graphs. ''''
Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein

The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We demonstrate that specific mutations in the ATP‐binding domain of a single Arabidopsis cytosolic HSP90 isoform compromise RPM1 function. These mutations do not affect the function of related disease resistance proteins. RPM1 associates with HSP90 in plant cells. The Arabidopsis proteins RAR1 and SGT1 are required for the action of many R proteins, and display some structural similarity to HSP90 co‐chaperones. Each associates with HSP90 in plant cells. Our data suggest that (i) RPM1 is an HSP90 client protein; and (ii) RAR1 and SGT1 may function independently as HSP90 cofactors. Dynamic interactions among these proteins can regulate RPM1 stability and function, perhaps similarly to the formation and regulation of animal steroid receptor complexes.
Transgenic Rescue of the LARGEmyd Mouse: A LARGE Therapeutic Window?

LARGE is a glycosyltransferase involved in glycosylation of α-dystroglycan (α-DG). Absence of this protein in the LARGEmyd mouse results in α-DG hypoglycosylation, and is associated with central nervous system abnormalities and progressive muscular dystrophy. Up-regulation of LARGE has previously been proposed as a therapy for the secondary dystroglycanopathies: overexpression in cells compensates for defects in multiple dystroglycanopathy genes. Counterintuitively, LARGE overexpression in an FKRP-deficient mouse exacerbates pathology, suggesting that modulation of α-DG glycosylation requires further investigation. Here we demonstrate that transgenic expression of human LARGE (LARGE-LV5) in the LARGEmyd mouse restores α-DG glycosylation (with marked hyperglycosylation in muscle) and that this corrects both the muscle pathology and brain architecture. By quantitative analyses of LARGE transcripts we also here show that levels of transgenic and endogenous LARGE in the brains of transgenic animals are comparable, but that the transgene is markedly overexpressed in heart and particularly skeletal muscle (20–100 fold over endogenous). Our data suggest LARGE overexpression may only be deleterious under a forced regenerative context, such as that resulting from a reduction in FKRP: in the absence of such a defect we show that systemic expression of LARGE can indeed act therapeutically, and that even dramatic LARGE overexpression is well-tolerated in heart and skeletal muscle. Moreover, correction of LARGEmyd brain pathology with only moderate, near-physiological LARGE expression suggests a generous therapeutic window.
Comparative Performance Analysis of XOR- XNOR Function Based High-Speed CMOS Full Adder Circuits For Low Voltage VLSI Design

This paper presents comparative study of high-speed, low-power and low voltage full adder circuits. Our approach is based on XOR-XNOR design full adder circuits in a single unit. A low power and high performance 9T full adder cell using a design style called “XOR (3T)” is discussed. The designed circuit commands a high degree of regularity and symmetric higher density than the conventional CMOS design style as well as it lowers power consumption by using XOR (3T) logic circuits. Gate Diffusion Input (GDI) technique of low-power digital combinatorial circuit design is also described. This technique helps in reducing the power consumption and the area of digital circuits while maintaining low complexity of logic design. This paper analyses, evaluates and compares the performance of various adder circuits. Several simulations conducted using different voltage supplies, load capacitors and temperature variation demonstrate the superiority of the XOR (3T) based full adder designs in term of delay, power and power delay product (PDP) compared to the other full adder circuits. Simulation results illustrate the superiority of the designed adder circuits against the conventional CMOS, TG and Hybrid full adder circuits in terms of power, delay and power delay product (PDP).
VANG-1 and PRKL-1 Cooperate to Negatively Regulate Neurite Formation in Caenorhabditis elegans

Neuritogenesis is a critical early step in the development and maturation of neurons and neuronal circuits. While extracellular directional cues are known to specify the site and orientation of nascent neurite formation in vivo, little is known about the genetic pathways that block inappropriate neurite emergence in order to maintain proper neuronal polarity. Here we report that the Caenorhabditis elegans orthologues of Van Gogh (vang-1), Prickle (prkl-1), and Dishevelled (dsh-1), core components of planar cell polarity (PCP) signaling, are required in a subset of peripheral motor neurons to restrict neurite emergence to a specific organ axis. In loss-of-function mutants, neurons display supernumerary neurites that extend inappropriately along the orthogonal anteroposterior (A/P) body axis. We show that autonomous and non-autonomous gene activities are required early and persistently to inhibit the formation or consolidation of growth cone protrusions directed away from organ precursor cells. Furthermore, prkl-1 overexpression is sufficient to suppress neurite formation and reorient neuronal polarity in a vang-1– and dsh-1–dependent manner. Our findings suggest a novel role for a PCP–like pathway in maintaining polarized neuronal morphology by inhibiting neuronal responses to extrinsic or intrinsic cues that would otherwise promote extraneous neurite formation.
THE UNORTHODOX ORBITS OF SUBSTRUCTURE HALOS

We use a suite of cosmological N-body simulations to study the properties of substructure halos (subhalos) in galaxy-sized cold dark matter halos. We extend prior work on the subject by considering the whole population of subhalos physically associated with the main system. These are defined as subhalos that have at some time in the past been within the virial radius of the halo's main progenitor and that have survived as self-bound entities to z = 0. We find that this population extends beyond three times the virial radius, and contains objects on extreme orbits, including a few with velocities approaching the nominal escape speed from the system. We trace the origin of these unorthodox orbits to the tidal dissociation of bound groups of subhalos, which results in the ejection of some subhalos along tidal streams. Ejected subhalos are primarily low-mass systems, leading to mass-dependent biases in their spatial distribution and kinematics: the lower the subhalo mass at accretion time, the less centrally concentrated and kinematically hotter their descendant population. The bias is strongest among the most massive subhalos, but disappears at the low-mass end: below a certain mass, subhalos behave just like test particles in the potential of the main halo. Overall, our findings imply that subhalos identified within the virial radius represent a rather incomplete census of the substructure physically related to a halo: only about one half of all associated subhalos are found today within the virial radius of a halo, and many relatively isolated halos may have actually been ejected in the past from more massive systems. These results may explain the age dependence of the clustering of low-mass halos reported recently by Gao et al., and has further implications for (1) the interpretation of the structural parameters and assembly histories of halos neighboring massive systems; (2) the existence of low-mass dynamical outliers, such as Leo I and And XII in the Local Group; and (3) the presence of evidence for evolutionary effects, such as tidal truncation or ram-pressure stripping, well outside the traditional virial boundary of a galaxy system.
1 KNOWLEDGE SHARING AND SOCIAL INTERACTION WITHIN MNCS

Social interaction between managers from different units of a multinational corporation (MNC) has been shown to be an important factor stimulating intra-MNC knowledge sharing. Face-to-face social interactions form a communication channel particularly conducive to the transfer of tacit, non-codified knowledge. But intensive social interaction also provides opportunities for social construction of knowledge in a learning dialogue. The first (sender-receiver) explanation makes us expect social interaction to positively moderate the effects of the factors giving rise to knowledge flows in the first place, such as differences in capabilities between MNC subsidiaries. The second (social learning) perspective also grants an independent effect to social interaction as a main factor stimulating intraMNC knowledge flows. We formulate hypotheses based on both perspectives, and test these on data from 169 MNC subsidiaries. Our findings show a considerable main effect of social interaction on all intra-MNC knowledge flows, confirming the expectations based on the social learning model. Interaction effects, based on the predictions of the sender-receiver model, are only partly confirmed. These findings suggest that future research should devote more attention to the social constitution of MNC knowledge.
Tactile responses of hindpaw, forepaw and whisker neurons in the thalamic ventrobasal complex of anesthetized rats

The majority of studies investigating responses of thalamocortical neurons to tactile stimuli have focused on the whisker representation of the rat thalamus: the ventral–posterior–medial nucleus (VPM). To test whether the basic properties of thalamocortical responses to tactile stimuli could be extended to the entire ventrobasal complex, we recorded single neurons from the whisker, forepaw and hindpaw thalamic representations. We performed a systematic analysis of responses to stereotyped tactile stimuli − 500 ms pulses (i.e. ON–OFF stimuli) or 1 ms pulses (i.e. impulsive stimuli) − under two different anesthetics (pentobarbital or urethane). We obtained the following main results: (i) the tuning of cells to ON vs. OFF stimuli displayed a gradient across neurons, so that two‐thirds of cells responded more to ON stimuli and one‐third responded more to OFF stimuli; (ii) on average, response magnitudes did not differ between ON and OFF stimuli, whereas latencies of response to OFF stimuli were a few milliseconds longer; (iii) latencies of response to ON and OFF stimuli were highly correlated; (iv) responses to impulsive stimuli and ON stimuli showed a strong correlation, whereas the relationship between the responses to impulsive stimuli and OFF stimuli was subtler; (v) unlike ON responses, OFF responses did not decrease when stimuli were moved from the receptive field center to a close location in the excitatory surround. We obtained the same results for hindpaw, forepaw and whisker neurons. Our results support the view of a neurophysiologically homogeneous ventrobasal complex, in which OFF responses participate in the structure of the spatiotemporal receptive field of thalamocortical neurons for tactile stimuli.
Scarless healing: Oral mucosa as a scientific model

Cutaneous wound healing's usual endpoint is scar formation. In contrast to the skin, the oral mucosa heals in a scarless manner, despite undergoing the same stages of the healing process. While wound environment may play a part, studies have demonstrated that inherent differences in the phenotypic and genotypic characteristics of the resident fibroblast populations may have a role in mediating this differential healing. This article analyses the evidence surrounding scarless healing in the oral mucosa and discusses future areas of research and highlights the potential clinical implications of this fascinating phenomenon.
Properties of single voltage‐dependent K+ channels in dendrites of CA1 pyramidal neurones of rat hippocampus

Voltage‐dependent K+ channels in the apical dendrites of CA1 pyramidal neurones play important roles in regulating dendritic excitability, synaptic integration, and synaptic plasticity. Using cell‐attached, voltage‐clamp recordings, we found a large variability in the waveforms of macroscopic K+ currents in the dendrites. With single‐channel analysis, however, we were able to identify four types of voltage‐dependent K+ channels and we categorized them as belonging to delayed‐rectifier, M‐, D‐, or A‐type K+ channels previously described from whole‐cell recordings. Delayed‐rectifier‐type K+ channels had a single‐channel conductance of 19 ± 0.5 pS, and made up the majority of the sustained K+ current uniformly distributed along the apical dendrites. The M‐type K+ channels had a single‐channel conductance of 11 ± 0.8 pS, did not inactivate with prolonged membrane depolarization, deactivated with slow kinetics (time constant 100 ± 6 ms at −40 mV), and were inhibited by bath‐applied muscarinic agonist carbachol (10 μm). The D‐type K+ channels had a single‐channel conductance of around 18 pS, and inactivated with a time constant of 98 ± 4 ms at +54 mV. The A‐type K+ channels had a single‐channel conductance of 6 ± 0.6 pS, inactivated with a time constant of 23 ± 2 ms at +54 mV, and contributed to the majority of the transient K+ current previously described. These results suggest both functional and molecular complexity for K+ channels in dendrites of CA1 pyramidal neurones.
Interaction between Hormonal Receptor Status, Age and Survival in Patients with BRCA1/2 Germline Mutations: A Systematic Review and Meta-Regression

Background Germline mutations in the BRCA1 and BRCA2 genes are the most frequent known hereditary causes of familial breast cancer. Little is known about the interaction of age at diagnosis, estrogen receptor (ER) and progesterone receptor (PgR) expression and outcomes in patients with BRCA1 or BRCA2 mutations. Methods A PubMed search identified publications exploring the association between BRCA mutations and clinical outcome. Hazard ratios (HR) for overall survival were extracted from multivariable analyses. Hazard ratios were weighted and pooled using generic inverse-variance and random-effect modeling. Meta-regression weighted by total study sample size was conducted to explore the influence of age, ER and PgR expression on the association between BRCA mutations and overall survival. Results A total of 16 studies comprising 10,180 patients were included in the analyses. BRCA mutations were not associated with worse overall survival (HR 1.06, 95% CI 0.84–1.34, p = 0.61). A similar finding was observed when evaluating the influence of BRCA1 and BRCA2 mutations on overall survival independently (BRCA1: HR 1.20, 95% CI 0.89–1.61, p = 0.24; BRCA2: HR 1.01, 95% CI 0.80–1.27, p = 0.95). Meta-regression identified an inverse association between ER expression and overall survival (β = -0.75, p = 0.02) in BRCA1 mutation carriers but no association with age or PgR expression (β = -0.45, p = 0.23 and β = 0.02, p = 0.97, respectively). No association was found for BRCA2 mutation status and age, ER, or PgR expression. Conclusion ER-expression appears to be an effect modifier in patients with BRCA1 mutations, but not among those with BRCA2 mutations.
Learning Collaborative Sparse Representation for Grayscale-Thermal Tracking

Integrating multiple different yet complementary feature representations has been proved to be an effective way for boosting tracking performance. This paper investigates how to perform robust object tracking in challenging scenarios by adaptively incorporating information from grayscale and thermal videos, and proposes a novel collaborative algorithm for online tracking. In particular, an adaptive fusion scheme is proposed based on collaborative sparse representation in Bayesian filtering framework. We jointly optimize sparse codes and the reliable weights of different modalities in an online way. In addition, this paper contributes a comprehensive video benchmark, which includes 50 grayscale-thermal sequences and their ground truth annotations for tracking purpose. The videos are with high diversity and the annotations were finished by one single person to guarantee consistency. Extensive experiments against other state-of-the-art trackers with both grayscale and grayscale-thermal inputs demonstrate the effectiveness of the proposed tracking approach. Through analyzing quantitative results, we also provide basic insights and potential future research directions in grayscale-thermal tracking.
Anomalous Effect of Permeant Ion Concentration on Peak Open Probability of Cardiac Na+ Channels

Human heart Na+ channels were expressed transiently in both mammalian cells and Xenopus oocytes, and Na+ currents measured using 150 mM intracellular Na+. Decreasing extracellular permeant ion concentration decreases outward Na+ current at positive voltages while increasing the driving force for the current. This anomalous effect of permeant ion concentration, especially obvious in a mutant (F1485Q) in which fast inactivation is partially abolished, is due to an alteration of open probability. The effect is only observed when a highly permeant cation (Na+, Li+, or hydrazinium) is substituted for a relatively impermeant cation (K+, Rb+, Cs+, N -methylglucamine, Tris, choline, or tetramethylammonium). With high concentrations of extracellular permeant cations, the peak open probability of Na+ channels increases with depolarization and then saturates at positive voltages. By contrast, with low concentrations of permeant ions, the open probability reaches a maximum at approximately 0 mV and then decreases with further depolarization. There is little effect of permeant ion concentration on activation kinetics at depolarized voltages. Furthermore, the lowered open probability caused by a brief depolarization to +60 mV recovers within 5 ms upon repolarization to −140 mV, indicative of a gating process with rapid kinetics. Tail currents at reduced temperatures reveal the rapid onset of this gating process during a large depolarization. A large depolarization may drive a permeant cation out of a site within the extracellular mouth of the pore, reducing the efficiency with which the channel opens.
Causes and Correlates of Calf Mortality in Captive Asian Elephants (Elephas maximus)

Juvenile mortality is a key factor influencing population growth rate in density-independent, predation-free, well-managed captive populations. Currently at least a quarter of all Asian elephants live in captivity, but both the wild and captive populations are unsustainable with the present fertility and calf mortality rates. Despite the need for detailed data on calf mortality to manage effectively populations and to minimize the need for capture from the wild, very little is known of the causes and correlates of calf mortality in Asian elephants. Here we use the world's largest multigenerational demographic dataset on a semi-captive population of Asian elephants compiled from timber camps in Myanmar to investigate the survival of calves (n = 1020) to age five born to captive-born mothers (n = 391) between 1960 and 1999. Mortality risk varied significantly across different ages and was higher for males at any age. Maternal reproductive history was associated with large differences in both stillbirth and liveborn mortality risk: first-time mothers had a higher risk of calf loss as did mothers producing another calf soon (<3.7 years) after a previous birth, and when giving birth at older age. Stillbirth (4%) and pre-weaning mortality (25.6%) were considerably lower than those reported for zoo elephants and used in published population viability analyses. A large proportion of deaths were caused by accidents and lack of maternal milk/calf weakness which both might be partly preventable by supplementary feeding of mothers and calves and work reduction of high-risk mothers. Our results on Myanmar timber elephants with an extensive keeping system provide an important comparison to compromised survivorship reported in zoo elephants. They have implications for improving captive working elephant management systems in range countries and for refining population viability analyses with realistic parameter values in order to predict future population size of the Asian elephant.