Miracle-dz/UniFAR-FTUs huggingface dataset:
The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter

The transcription rate of the dihydrofolate reductase (DHFR) gene increases at the G1/S boundary of the proliferative cell cycle. Through analysis of transiently and stably transfected NIH 3T3 cells, we have now demonstrated that DHFR promoter sequences extending from -270 to +20 are sufficient to confer similar regulation on a reporter gene. Mutation of a protein binding site that spans sequences from -16 to +11 in the DHFR promoter resulted in loss of the transcriptional increase at the G1/S boundary. Purification of an activity from HeLa nuclear extract that binds to this region enriched for a 180-kDa polypeptide (HIP1). Using this HIP1 preparation, we have identified specific positions within the binding site that are critical for efficient protein-DNA interactions. An analysis of association and dissociation rates suggests that bound HIP1 protein can exchange rapidly with free protein. This rapid exchange may facilitate the burst of transcriptional activity from the DHFR promoter at the G1/S boundary.
Interpreting ADHD Rating Scale Scores: Linking ADHD Rating Scale Scores and CGI Levels in Two Randomized Controlled Trials of Lisdexamfetamine Dimesylate in ADHD

Objective: To provide additional understanding of the clinical significance of Attention-Deficit/Hyperactivity Disorder Rating Scale, Version IV (ADHD-RS-IV) total and change scores in relation to Clinical Global Impressions-Severity or -Improvement (CGI-S/-I) levels. Methods: Using two similarly designed pivotal trials of lisdexamfetamine dimesylate (Vyvanse, Shire US Inc), equipercentile linking was used to identify scores on the ADHD-RS-IV and CGI that have the same percentile rank. Results: As assessed by CGI-S levels, moderately, markedly, severely, and extremely ill adults had mean (SD) baseline ADHD-RS-IV scores of 36.2 (4.9), 42.1 (6.1), 45.4 (5.1), and 53.0, respectively. A similar relationship was observed in children. At endpoint, children categorized as minimally, much, or very much improved by CGI-I demonstrated mean (SD) ADHD-RS-IV changes from baseline of -9.9 (6.8), -25.5 (7.2), and -33.2 (9.3), respectively. Adults demonstrated a similar relationship between ADHD-RS-IV change scores and CGI-I ratings. Based on equipercentile link function, a change from baseline in ADHD-RS-IV total score of ~10–15 points or 25% to 30% corresponded to a change of 1 level in CGI-I score. Conclusion: This analysis makes possible the establishment of a clinical impression of severity of illness from total ADHD-RS-IV scores and may facilitate the clinical interpretation of improvement of ADHD-RS-IV change scores.
DETERMINATION OF THE SLIP SYSTEMS IN SINGLE CRYSTALS OF TUNGSTEN MONOCARBIDE

Abstract Slip in single crystals of tungsten monocarbide has been observed around Vickers pyramid hardness indentations. The slip plane is {l100} at room temperature and the slip directions are postulated to be the and . The Vickers hardness value on a (0001) face is approximately twice the value determined for a (1100) face. This is attributed to the ease of motion of dislocations with Burgers vectors as opposed to the dislocations having Burgers vectors since the former can dissociate into partial dislocations. The occurrence of a {1100} slip plane is explained on the basis of the fact that a minimum number of only two covalent tungsten-carbon bonds need be broken for a unit of slip on this plane.
Longitudinal assessment of mental health problems among active and reserve component soldiers returning from the Iraq war.

CONTEXT To promote early identification of mental health problems among combat veterans, the Department of Defense initiated population-wide screening at 2 time points, immediately on return from deployment and 3 to 6 months later. A previous article focusing only on the initial screening is likely to have underestimated the mental health burden. OBJECTIVE To measure the mental health needs among soldiers returning from Iraq and the association of screening with mental health care utilization. DESIGN, SETTING, AND PARTICIPANTS Population-based, longitudinal descriptive study of the initial large cohort of 88 235 US soldiers returning from Iraq who completed both a Post-Deployment Health Assessment (PDHA) and a Post-Deployment Health Re-Assessment (PDHRA) with a median of 6 months between the 2 assessments. MAIN OUTCOME MEASURES Screening positive for posttraumatic stress disorder (PTSD), major depression, alcohol misuse, or other mental health problems; referral and use of mental health services. RESULTS Soldiers reported more mental health concerns and were referred at significantly higher rates from the PDHRA than from the PDHA. Based on the combined screening, clinicians identified 20.3% of active and 42.4% of reserve component soldiers as requiring mental health treatment. Concerns about interpersonal conflict increased 4-fold. Soldiers frequently reported alcohol concerns, yet very few were referred to alcohol treatment. Most soldiers who used mental health services had not been referred, even though the majority accessed care within 30 days following the screening. Although soldiers were much more likely to report PTSD symptoms on the PDHRA than on the PDHA, 49% to 59% of those who had PTSD symptoms identified on the PDHA improved by the time they took the PDHRA. There was no direct relationship of referral or treatment with symptom improvement. CONCLUSIONS Rescreening soldiers several months after their return from Iraq identified a large cohort missed on initial screening. The large clinical burden recently reported among veterans presenting to Veterans Affairs facilities seems to exist within months of returning home, highlighting the need to enhance military mental health care during this period. Increased relationship problems underscore shortcomings in services for family members. Reserve component soldiers who had returned to civilian status were referred at higher rates on the PDHRA, which could reflect their concerns about their ongoing health coverage. Lack of confidentiality may deter soldiers with alcohol problems from accessing treatment. In the context of an overburdened system of care, the effectiveness of population mental health screening was difficult to ascertain.
Melamine sensing based on evanescent field enhanced optical fiber sensor

Melamine is an insalubrious chemical, and has been frequently added into milk products illegally, to make the products more protein-rich. However, it can cause some various diseases, such as kidney stones and bladder cancer. In this paper, a novel optical fiber sensor with high sensitivity based on absorption of the evanescent field for melamine detection is successfully proposed and developed. Different concentrations of melamine changing from 0 to 10mg/mL have been detected using the micro/nano-sensing fiber decorated with silver nanoparticles cluster layer. As the concentration increases, the sensing fiber’s output intensity gradually deceases and the absorption of the analyte becomes large. The concentration changing of 1mg/ml can cause the absorbance varying 0.664 and the limit of the melamine detectable concentration is 1ug/mL. Besides, the coupling properties between silver nanoparticles have also been analyzed by the FDTD method. Overall, this evanescent field enhanced optical fiber sensor has potential to be used in oligo-analyte detection and will promote the development of biomolecular and chemical sensing applications.
Modular coordination of multiple autonomic managers

Complex computing systems are increasingly self-adaptive, with an autonomic computing approach for their administration. Real systems require the co-existence of multiple autonomic management loops, each complex to design. However their uncoordinated co-existence leads to performance degradation and possibly to inconsistency. There is a need for methodological supports facilitating the coordination of multiple autonomic managers. In this paper we propose a method focusing on the discrete control of the interactions of managers. We follow a component-based approach and explore modular discrete control, allowing to break down the combinatorial complexity inherent to the state-space exploration technique. This improves scalability of the approach and allows constructing a hierarchical control. It also allows re-using complex managers in different contexts without modifying their control specifications. We build a component-based coordination of managers, with introspection, adaptivity and reconfiguration. We validate our method on a multiple-loop multi-tier system.
Story of survival in anaplastic large cell lymphoma - sometimes more than the anaplastic lymphoma kinase status: An evaluation of pathologic prognostic factors in 102 cases

Introduction: Systemic anaplastic large cell lymphoma (ALCL) accounts for 5%–10% of adult non Hodgkin's lymphoma (NHL) and 10%–30% of childhood NHL. Owing to significant differences in survival and gene expression profile, current WHO classifies ALCL into two distinct entities as anaplastic lymphoma receptor tyrosine kinase (ALK) positive and ALK negative ALCL with ALK expression by tumour as a good prognostic indicator. However, in our institute which is a cancer referral institute, our preliminary experience was that even ALK positive tumours did not fare well as compared to ALK- negative ALCL. So, the current study aims at exploring more clinical and pathological factors impacting survival in ALCL patients. Objective: To study clinical and pathological prognostic factors in cases of ALCL. Methods: 102 cases of ALCL were retrieved from pathology database. Pathological features and clinical features of these cases were recorded and factors found to impact overall survival (OAS) and disease-free survival (DFS) curves were identified based on univariate and multivariate analysis. Results: ALK 1 expression was seen in 71/102 (69.6%) cases and was not found to impact OAS or DFS. The 2 year OAS rate for ALK positive patients was 63.5% and DFS rate was 54.4%, while for ALK negative patients, the OAS was 60.5% and DFS was 43.5%. The Ann Arbor stage, performance status, international prognostic index, histological subtype, and the degree of the background inflammatory infiltrate were found to impact the OAS significantly. Increased reactive inflammatory component also negatively impacted DFS. In the multivariate analysis, only the histologic type emerged as significant for OAS. Conclusion: Though ALK plays a role in prognostication of systemic ALCL, advanced stage disease and an inflammatory milieu may modulate the final outcome. We report a study of clinical and pathologic prognostic features in 102 cases of anaplastic large cell lymphoma (ALCL) from a cancer referral institute in India. Anaplastic lymphoma receptor tyrosine kinase (ALK-1) expression was seen in 71/102 (69.6%) cases and was not found to impact overall survival (OAS) or disease-free survival (DFS). The 2-year OAS rate for ALK-positive patients was 63.5% and DFS rate was 54.4%, while for ALK-negative patients, the OAS was 60.5% and DFS was 43.5%. The Ann Arbor stage, performance status, international prognostic index, histological subtype, and the degree of the background inflammatory infiltrate were found to impact the OAS significantly. Increased reactive inflammatory component also negatively impacted DFS. In the multivariate analysis, only the histologic type emerged as significant for OAS. Thus, though ALK plays a role in prognostication of systemic ALCL, advanced stage disease and an inflammatory milieu may modulate the final outcome.
Long-term survival after liver transplantation in patients with familial amyloid polyneuropathy

Objective: Familial amyloid polyneuropathy (FAP), which is a fatal disorder inherited in an autosomal dominant fashion, is characterized by systemic accumulation of polymerized transthyretin (TTR) in the peripheral nerves and systemic organs. Liver transplantation has become an accepted treatment of this disorder because it stops the major production of amyloidogenic TTR. However, improved survival of transplant patients compared with that of nontransplant patients has not been sufficiently demonstrated. This study investigated whether transplantation improved the long-term outcome of patients by comparing the survival of patients who had transplantations with that of patients who had not had transplantations. Methods: Eighty consecutive patients with FAP Val30Met who visited Kumamoto University Hospital between January 1990 and December 2010 were studied. The transplant group consisted of 37 patients who had a partial hepatic graft via living donor transplantation in Japan or who underwent liver transplantation in Sweden, Australia, or the United States. The nontransplant group consisted of 43 patients with FAP. Survival was evaluated by using Kaplan-Meier analysis, and the difference in survival was examined via the log-rank test. Results: The transplant group had prolonged survival (p < 0.001) compared with the nontransplant group. The estimated probability of survival at 10 years was 56.1% for the nontransplant group vs 100% for the transplant group. Conclusion: Liver transplantation should be considered as an effective treatment in clinical management of patients with FAP Val30Met. Classification of evidence: This study provides Class III evidence that liver transplantation prolongs survival in patients with FAP Val30Met.
Development of bismuth ferrite derived piezoelectric ceramics for high temperature applications

The foundations of this project lay in the authors research into the xBiFeO3-(1-x)(K0.5Bi0.5)TiO3 ceramic system. A broad pseudocubic region was identified where x = 0.4-0.3, these compositions produced large electric-field induced strains (>0.3%) due to the presence of polar nanoregions that are easily switched by an applied electric field, however, these return to a random order upon removal of the field. Positive-up negative-down (PUND) showed that ferroelectric switching was reduced in the pseudocubic region and synchrotron studies with an applied electric-field in-situ demonstrated predominantly reversible domain switching. Solid-solutions based upon the (1-x-y)BiFeO3-x(K0.5Bi0.5)TiO3-yPbTiO3 ternary system have been fabricated by conventional mixed oxide processing, this system is the primary focus of this thesis. Structural analysis using XRD established that long-range crystallographic order was present above a critical PbTiO3 concentration, in addition a broad mixed symmetry region was identified comprised of tetragonal and rhombohedral symmetries. The ferroelectric Curie temperature was between 410-590 oC. Conventional ferroelectric and piezoelectric ordering was observed with the use of strain and polarization-field measurements. Rayleigh analysis was used to quantify the intrinsic and extrinsic contributions, a critical c/a ratio was identified at 1.045 with domain switching limited above this. Synchrotron x-rays with ex-situ electric-fields allowed for a more detailed structural analysis, selected compositions demonstrated significant phase changing behaviour. The room temperature tetragonal c/a ratio across the mixed symmetry region, Curie temperature, and piezoelectric d33 were found to correlate strongly with the PbTiO3 concentration in a number of compositions. This work demonstrates a number of important results that have wider implications. Ternary and pseudo-quaternary systems have recently been subject to large amounts of research as many of the possible binary systems have been exhausted. This has led to a raft of materials with reported high electric-field induced strains, these are often electrostrictive or have limited operating temperatures. The partial substitution of PbTiO3 promotes long-range non-centrosymmetric order, which leads to increased piezoelectric activity and TC.
Title Ammonia-oxidizing archaea and ammonia-oxidizing bacteria insix full-scale wastewater treatment bioreactors

In this study, dideoxy sequencing and 454 highthroughput sequencing were used to analyze diversities of the ammonia monooxygenase (amoA) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amoA genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amoA and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae, Nitrosomonas oligotropha, Nitrosomonas marina, and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amoA genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amoA and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low.
A Maximum Entropy Joint Demand Estimation and Capacity Control Policy

We propose a tractable, data‐driven demand estimation procedure based on the use of maximum entropy (ME) distributions, and apply it to a stochastic capacity control problem motivated from airline revenue management. Specifically, we study the two fare class “Littlewood” problem in a setting where the firm has access to only potentially censored sales observations; this is also known as the repeated newsvendor problem. We propose a heuristic that iteratively fits an ME distribution to all observed sales data, and in each iteration selects a protection level based on the estimated distribution. When the underlying demand distribution is discrete, we show that the sequence of protection levels converges to the optimal one almost surely, and that the ME demand forecast converges to the true demand distribution for all values below the optimal protection level. That is, the proposed heuristic avoids the “spiral down” effect, making it attractive for problems of joint forecasting and revenue optimization problems in the presence of censored observations.
Human papillomavirus E6/E7 mRNA testing as a predictive marker for cervical carcinoma

Human papillomavirus (HPV) is necessary for the development of cervical carcinoma, and incorporation of molecular testing for HPV in screening and patient management has been proposed. Sufficient scientific evidence exists to recommend HPV DNA testing in the triage of women with equivocal cytology and in follow-up after the treatment of precursor lesions. However, due to a low clinical specificity and positive predictive value, HPV DNA testing has so far not been recommended as primary screening in Europe. In general, diagnostic HPV tests have to demonstrate accuracy, reproducibility and clinical utility before they can be used in patient management and implemented in cervical cancer screening programmes. In this article we give an overview of RNA-based HPV diagnostics and the role of E6/E7 mRNA detection as a predictive marker for the development of cervical carcinoma. HPV E6/E7 mRNA testing for high-risk types seems to correlate better with the severity of the lesion compared with HPV DNA testing, and is a potential marker for the identification of women at risk of developing cervical carcinoma. Commercial assays for simultaneous genotyping and detection of E6/E7 mRNA from the five most common high-risk HPV types are now available and require further evaluation for primary screening, triage and follow-up after treatment.
Micromagnetic Simulation of Magnetic Hysteresis in Hard/Soft Bilayered Exchange Spring Magnets

We investigated the magnetization switching process in a bilayered exchange spring system through micromagnetic simulations. A typical exchange coupled magnetic hysteresis loop was observed in this work. The influence of magnetocrystalline anisotropy constant on the magnetization of the exchange spring structure was also studied. It is demonstrated that the film thickness of hard layer play a more important role than that of soft layer to the influence on coercive field and shape of the hysteresis loop.
Techniques to measure miniscrew implant stability

The use of miniscrews for anchorage control during orthodontic treatment has the potential to improve the treatment of certain types of malocclusions. However, miniscrew failures will greatly influence the efficiency and efficacy of treatment. Having a better understanding of the healing process that occurs around miniscrew implants will provide valuable information that could enhance the predictability of their use. There are several methods to evaluate the stability. These fall into two main groups, which are invasive techniques and non-invasive techniques. The non-invasive measurement technique, resonance frequency analysis, holds great promise for the clinical evaluation of miniscrew implant stability. It may be used to evaluate the transition from primary to secondary stability, producing a better understanding of the time periods that are high risk for screw failure. The technique also provides a method to determine the effect that a modification of the placement protocol of miniscrew implants might have on the transition from primary to secondary stability. On that basis, this review will cover the methods to evaluate miniscrew implant stability longitudinally, both in vitro and in vivo .
From Freedom to Involvement: On the Rhetoric of Mobility in HCI Research

The concept of 'mobility' as it is conceptualized in mobile HCI is scrutinized in this paper. The currently applied understanding is often limited to perceiving mobility as corporeal and in spatial and temporal terms exclusively. While some have attempted to include contextual and social dimensions, their ways of approaching this issue seems problematic and in fact only continue a far-reaching separation between the physical and what is seen as the social or the subjective. These should however not be seen as disparate but rather as co-creators of what one perceives as 'reality'. The concept of involvement from phenomenology is introduced to discuss the possibilities of changing contexts to which use of mobile information technology gives rise. To conclude, we argue that mobile HCI needs to be thought of as designing for involvement in these diverse physio-social contexts, rather than as designing technology with a spatial and temporal location.
Quantum dots as phototoxic drugs and sensors of specific metabolic processes in living cells

When conjugated to CdSe/ZnS nanocrystals (quantum dots), the nucleobase adenine and the neurotransmitter dopamine quench fluorescence emission from in a manner strongly dependent upon the size of the quantum dot. The degree of quenching serves to predict the efficiency with which the conjugates are able to enter living cells. Along with quenching, the presence of specific receptors on the cells is necessary for QD binding, entry, and phototoxicity. Toxicity is manifested by opening of large membrane pores and by oxidative DNA damage, and does not require the release of Cd+2. In bacterial cells, light exposure is necessary for uptake, and procedures to reduce toxicity eliminate labeling. In mammalian cells, antioxidants prevent toxicity but not QD uptake, leading to QD-loaded cells that are nonfluorescent before light exposure. These findings provide a general procedure for rational design of nanoparticle-based photosensitizing drugs and for "off-on" fluorescent labels