Miracle-dz/UniFAR-FTUs huggingface dataset:
A CRITICAL EVALUATION OF RED CELL AND PLASMA VOLUME TECHNIQUES IN PATIENTS WITH CIVILIAN INJURIES

There is now much evidence to suggest that the major cause of red cell disappearance following trauma is haemorrhage. After severe skeletal injuries, an abating but often clinically important bleed continues for one to four days (Clarke, Davies, Fisher, and Topley, 1959). It can be partly appreciated by the nature of the injury and a judgment of the external blood loss and internal swelling, the early swelling often being entirely due to haemorrhage. The local bleeding can also be assessed indirectly by an estimation of the amount of blood lost from the general circulation. The major cause of " wound shock " is now realized frequently to be a low blood volume, itself caused by blood loss unreplaced by blood transfusion. The effect of the low blood volume can therefore be used as a crude guide to possible haemorrhage. The level of the pulse and blood pressure (Fisher, 1958) and the changing level of the external jugular vein are of limited value as a guide to total blood volume. By these multiple approaches the experienced surgeon often assesses the timing and degree of haemorrhage to within I to 1 litre of whole blood (10-20% of the normal blood volume of a man 170 cm. tall). But there are occasions where all these guides are too crude; a haemorrhage of a litre or more of blood could be overlooked; and the emergency management of the injury requires a greater precision in the assessment of haemorrhage. It is under these conditions that the laboratory can often assist by measuring the circulating red cell volume. This red cell volume estimate can be compared with the average normal red cell volume estimated from the height. A "red cell volume estimate of haemorrhage" (Topley, Fisher, Davies, and Clarke, 1959) can be obtained by subtracting the volume of blood found to be remaining in the patient from that assumed to be present before
Pharmacokinetics of HM-3 After Intravitreal Administration in Mice

Abstract Purpose: HM-3, an RGD-modified endostatin-derived polypeptide, is a potent angiogenesis inhibitor synthesized in our laboratory. This study investigated the HM-3 pharmacokinetics of intravitreally administered in mice eyes as an anti-angiogenesis drug for age-related macular degeneration. Materials and methods: A total of 288 C57BL/6J mice were evaluated and divided into four groups. Each mouse in different groups received single bilateral intravitreal injection with HM-3. The concentrations of HM-3 in choroid/sclera, retina and serum were determined by indirect competitive enzyme-linked immunosorbent assay. Results: After intravitreal administration of doses of 0, 10, 20 and 40 μg/eye HM-3, the observed maximum concentration (Cmax) was 12.98 ± 1.42, 27.87 ± 3.64 and 55.96 ± 11.94 ng/mg, respectively; and the total area under the curve (AUCtot) was 739.23 ± 190.32, 1171.74 ± 528.75 and 1777.71 ± 511.64 h ng/mg; the elimination half-life (T1/2) in retina was 104.85 ± 36.90, 107.42 ± 35.25 and 101.12 ± 15.82 h; the mean residence time (MRT) was 172.46 ± 63.80, 164.70 ± 52.72 and 181.32 ± 26.01 h, respectively. In choroid/sclera, the Cmax was 5.29 ± 0.34, 6.29 ± 1.87 and 8.14 ± 0.71 ng/mg, respectively; AUCtot was 579.03 ± 56.50, 762.20 ± 201.09 and 720.91 ±243.87 h ng/mg; T1/2 was 54.04 ± 25.99, 59.33 ± 24.46 and 47.10 ± 10.00 h, respectively; MRT was 139.98 ± 23.93, 155.43 ± 17.81 and 136.45 ± 18.17 h, respectively. But in serum, the Cmax was 482.00 ± 38.97, 493.94 ± 97.64 and 1033.10 ± 276.33 ng/ml, respectively; AUCtot was 21128.55 ± 4683.68, 53444.57 ± 16963.99 and 53164.84 ±1535.06 h ng/ml; T1/2 was 48.39 ± 14.89, 47.96 ± 12.97 and 49.98 ± 30.07 h, respectively; MRT was 108.6 ± 47.17, 159.76 ± 18.82 and 125.33 ± 21.41 h, respectively. Conclusions: The pharmacokinetic profiles of intravitreal administration HM-3 provide the basis for the development of reasonable dosing regimens of clinical choroidal neovascularization (CNV) treatment. However, the vitreous and blood retinal barrier might be barriers to drug distribution and diffusion. In addition, fluid flow for the anterior transport and choroidal blood circulation might play important roles for multiple peaking. Carrying out the research into pharmacokinetics of HM-3 provides the information for laying down drug delivery scheme in mice model of CNV.
Estimating contact dynamics

Motion and interaction with the environment are fundamentally intertwined. Few people-tracking algorithms exploit such interactions, and those that do assume that surface geometry and dynamics are given. This paper concerns the converse problem, i.e., the inference of contact and environment properties from motion. For 3D human motion, with a 12-segment articulated body model, we show how one can estimate the forces acting on the body in terms of internal forces (joint torques), gravity, and the parameters of a contact model (e.g., the geometry and dynamics of a spring-based model). This is tested on motion capture data and video-based tracking data, with walking, jogging, cartwheels, and jumping.
Complexity of Inference in Topic Models

We consider the computational complexity of finding the MAP assignment of topics to words in Latent Dirichlet Allocation. We show that, when the effective number of topics per document is small, exact inference takes polynomial time. In contrast, we show that, when a document has a large number of topics, finding the MAP assignment in LDA is NP-hard. Our results motivate further study of the structure in real-world topic models, and raise a number of questions about the requirements for accurate inference during both learning and test-time use of topic models.
Assessing Errors Inherent in OCT-Derived Macular Thickness Maps

SD-OCT has become an essential tool for evaluating macular pathology; however several aspects of data collection and analysis affect the accuracy of retinal thickness measurements. Here we evaluated sampling density, scan centering, and axial length compensation as factors affecting the accuracy of macular thickness maps. Forty-three patients with various retinal pathologies and 113 normal subjects were imaged using Cirrus HD-OCT. Reduced B-scan density was associated with increased interpolation error in ETDRS macular thickness plots. Correcting for individual differences in axial length revealed modest errors in retinal thickness maps, while more pronounced errors were observed when the ETDRS plot was not positioned at the center of the fovea (which can occur as a result of errant fixation). Cumulative error can exceed hundreds of microns, even under “ideal observer” conditions. This preventable error is particularly relevant when attempting to compare macular thickness maps to normative databases or measuring the area or volume of retinal features.
Research Data Management Training for Geographers: First Impressions

Sharing and secondary analysis of data have become increasingly important for research. Especially in geography, the collection of digital data has grown due to technological changes. Responsible handling and proper documentation of research data have therefore become essential for funders, publishers and higher education institutions. To achieve this goal, universities offer support and training in research data management. This article presents the experiences of a pilot workshop in research data management, especially for geographers. A discipline-specific approach to research data management training is recommended. The focus of this approach increases researchers’ interest and allows for more specific guidance. The instructors identified problems and challenges of research data management for geographers. In regards to training, the communication of benefits and reaching the target groups seem to be the biggest challenges. Consequently, better incentive structures as well as communication channels have to be established.
The natural history of encephalomyocarditis virus-induced myositis and myocarditis in mice. Viral persistence demonstrated by in situ hybridization

Picornaviruses can initiate chronic inflammation that persists after the virus can no longer be cultured from inflamed tissues. In an attempt to understand this transition we have sought evidence for viral persistence by methods that detect viral genome independent of whether or not whole competent virus is present. In mice infected with a myotropic variant of encephalomyocarditis virus, EMC-221A, virus can be cultured in high yield at 1 wk and in low yield at 2 wk from skeletal muscle, heart, and brain; a small number of plaque-forming units could be cultured from brain at 4 wk. By contrast, in situ hybridization detected viral nucleic acid at least a week or two thereafter, often in single cells. In the skeletal muscle, inflammation disappeared by 3 wk, but in heart it remained for the full 12 wk of observation. In the brain, microglial nodules, sometimes with associated viral nucleic acid, were present for a long period. Application of this technique allows a more accurate assessment of the role of viral persistence in the pathogenesis of virus-initiated but apparently autoimmune inflammation.
Reshaping the Principalship: Insights From Transformational Reform Efforts

PART ONE: SETTING THE STAGE Reconstructing the Principalship - Joseph Murphy and Lynn G Beck Challenges and Possibilities Transformational Change and the Evolving Role of the Principal - Joseph Murphy Early Empirical Evidence PART TWO: CASE STUDIES The Principal as Mini-Superintendent Under Chicago School Reform - R Bruce McPherson and Robert L Crowson Transformational Leadership and Teachers' Commitment to Change - Kenneth Leithwood, Doris Jantzi and Alicia Fernandez School Leadership and Teacher Quality of Work Life in Restructuring Schools - Sheila Rosenblum, Karen Seashore Louis and Richard A Rossmiller Ninety Degrees from Everywhere - Nona A Prestine New Understandings of the Principal's Role in a Restructuring Essential School From Attila the Hun to Mary Had a Little Lamb - Philip Hallinger and Charles Hausman Principal Role Ambiguity in Restructured Schools Cultivating a Caring School Community - Lynn G Beck One Principal's Story Voices of Principals from Democratically Transformed Schools - Carl D Glickman, Lewis R Allen and Barbara F Lunsford Changes in School Governance and Principals' Roles - Kent D Peterson and Valli D Warren Changing Jurisdictions, New Power Dynamics, and Conflict in Restructured Schools Ten Propositions for Facilitative Leadership - David T Conley and Paul Goldman PART THREE: CONCLUSION The Evolving Role of the Principal - Karen Seashore Louis and Joseph Murphy Some Concluding Thoughts
Changes in functioning of mesolimbic incentive processing circuits during the premenstrual phase

The premenstrual phase of the menstrual cycle is associated with marked changes in normal and abnormal motivated behaviors. Animal studies suggest that such effects may result from actions of gonadal hormones on the mesolimbic dopamine (DA) system. We therefore investigated premenstrual changes in reward-related neural activity in terminal regions of the DA system in humans. Twenty-eight healthy young women underwent functional magnetic resonance imaging on 2 days during the menstrual cycle, once during the late follicular phase and once during the premenstrual phase, in counterbalanced order. Using a modified version of the monetary incentive delay task, we assessed responsiveness of the ventral striatum to reward anticipation. Our results show enhanced ventral striatal responses during the premenstrual as compared to the follicular phase. Moreover, this effect was most pronounced in women reporting more premenstrual symptoms. These findings provide support for the notion that changes in functioning of mesolimbic incentive processing circuits may underlie premenstrual changes in motivated behaviors. Notably, increases in reward-cue responsiveness have previously been associated with DA withdrawal states. Our findings therefore suggest that the sharp decline of gonadal hormone levels in the premenstrual phase may trigger a similar withdrawal-like state.
Alcohol and coronary heart disease: a meta-analysis.

OBJECTIVE To estimate parameters of the function relating alcohol consumption with the risk of coronary heart disease and to identify the sources of heterogeneity in the parameter estimates. METHODS A search of the epidemiological literature from 1966 to 1998 was performed using several bibliographic databases. Meta-regression models were fitted to evaluate non-linear effects of alcohol intake on the relative risk. The effects of some characteristics of the studies, including an index of their quality, were considered as putative sources of heterogeneity of the estimates. Publication bias was also investigated. FINDINGS Among the 196 initially reviewed articles, 51 were selected. Since qualitative characteristics of the studies were significant sources of heterogeneity, the pooled dose-response functions were based on the 28 cohort studies with higher quality. Risk decreased from 0 to 20 g/day (RR = 0.80; 95% CI: 0.78, 0.83); there was evidence of a protective effect up to 72 g/day (RR = 0.96; 95% CI: 0.92, 1.00) and increased risk above > or = 89 g/day (RR = 1.05; 95% CI: 1.00, 1.11). Lower protective effects and harmful effects were found in women, in men living in countries outside the Mediterranean area and in studies where fatal events were used as the outcome. Evidence of publication bias for moderate intakes and of heterogeneity of the estimates across studies for higher intakes were found. CONCLUSIONS The degree of protection from moderate doses of alcohol should be reconsidered. Further research investigating the effect of drinking patterns on the risk of coronary heart disease should be performed. Caution in making general recommendations is needed.
HLA Typing from 1000 Genomes Whole Genome and Whole Exome Illumina Data

Specific HLA genotypes are known to be linked to either resistance or susceptibility to certain diseases or sensitivity to certain drugs. In addition, high accuracy HLA typing is crucial for organ and bone marrow transplantation. The most widespread high resolution HLA typing method used to date is Sanger sequencing based typing (SBT), and next generation sequencing (NGS) based HLA typing is just starting to be adopted as a higher throughput, lower cost alternative. By HLA typing the HapMap subset of the public 1000 Genomes paired Illumina data, we demonstrate that HLA-A, B and C typing is possible from exome sequencing samples with higher than 90% accuracy. The older 1000 Genomes whole genome sequencing read sets are less reliable and generally unsuitable for the purpose of HLA typing. We also propose using coverage % (the extent of exons covered) as a quality check (QC) measure to increase reliability.
Reducing Fragmentation on 3D Torus-Based HPC Systems Using Packing-Based Job Scheduling and Job Placement Reconfiguration

We address the topology-aware job scheduling and placement problems on 3D torus-based high performance computing systems, with the objective of reducing system fragmentation. In our previous work, we proposed a job placement algorithm based on a local migration process, which aims at reducing the internal fragmentation due to using a convex prism shape for job allocation. However, HPC systems are prone to suffer from external fragmentation as well. Hence, in this paper, we majorly strive to reduce the external fragmentation brought in by the job scheduling and placement processes. Firstly, from the job scheduling aspect of view, we propose a packing-based job scheduling strategy, which reduces the external fragmentation by using the First Come First Served + backfilling strategy. Secondly, we give a review of the migration-based job placement algorithm in our previous work. Thirdly, in order to reduce the external fragmentation resulting from running jobs scattered across the system, we propose a job placement reconfiguration algorithm, which uses a global migration process to rearrange the placement of the running jobs across the system. Both local and global migration are emulated virtual processes under the off-line scenario, which have no migration overhead. However, under the on-line scenario, migration is a real process and leads to a migration delay. Therefore, we propose a buffer-based on-line scheduling model, which helps to avoid the delay of local migration. The evaluation results validate the efficiency of our approach in reducing system fragmentation and improving system utilization.
Search for Limiting Factors in the RNAi Pathway in Silkmoth Tissues and the Bm5 Cell Line: The RNA-Binding Proteins R2D2 and Translin

RNA interference (RNAi), an RNA-dependent gene silencing process that is initiated by double-stranded RNA (dsRNA) molecules, has been applied with variable success in lepidopteran insects, in contrast to the high efficiency achieved in the coleopteran Tribolium castaneum. To gain insight into the factors that determine the efficiency of RNAi, a survey was carried out to check the expression of factors that constitute the machinery of the small interfering RNA (siRNA) and microRNA (miRNA) pathways in different tissues and stages of the silkmoth, Bombyx mori. It was found that the dsRNA-binding protein R2D2, an essential component in the siRNA pathway in Drosophila, was expressed at minimal levels in silkmoth tissues. The silkmoth-derived Bm5 cell line was also deficient in expression of mRNA encoding full-length BmTranslin, an RNA-binding factor that has been shown to stimulate the efficiency of RNAi. However, despite the lack of expression of the RNA-binding proteins, silencing of a luciferase reporter gene was observed by co-transfection of luc dsRNA using a lipophilic reagent. In contrast, gene silencing was not detected when the cells were soaked in culture medium supplemented with dsRNA. The introduction of an expression construct for Tribolium R2D2 (TcR2D2) did not influence the potency of luc dsRNA to silence the luciferase reporter. Immunostaining experiments further showed that both TcR2D2 and BmTranslin accumulated at defined locations within the cytoplasm of transfected cells. Our results offer a first evaluation of the expression of the RNAi machinery in silkmoth tissues and Bm5 cells and provide evidence for a functional RNAi response to intracellular dsRNA in the absence of R2D2 and Translin. The failure of TcR2D2 to stimulate the intracellular RNAi pathway in Bombyx cells is discussed.
Envelopment of Human Cytomegalovirus Occurs by Budding into Golgi-Derived Vacuole Compartments Positive for gB, Rab 3, Trans-Golgi Network 46, and Mannosidase II

ABSTRACT Although considerable progress has been made towards characterizing virus assembly processes, assignment of the site of tegumentation and envelopment for human cytomegalovirus (HCMV) is still not clear. In this study, we examined the envelopment of HCMV particles in human lung fibroblasts (HF) HL 411 and HL 19, human umbilical vein endothelial cells, human pulmonary arterial endothelial cells, and arterial smooth muscle cells at different time points after infection by electron microscopy (EM), immunohistochemistry, and confocal microscopy analysis. Double-immunofluorescence labeling experiments demonstrated colocalization of the HCMV glycoprotein B (gB) with the Golgi resident enzyme mannosidase II, the Golgi marker TGN (trans-Golgi network) 46, and the secretory vacuole marker Rab 3 in all cell types investigated. Final envelopment of tegumented capsids was observed at 5 days postinfection by EM, when tegumented capsids budded into subcellular compartments located in the cytoplasm, in close proximity to the Golgi apparatus. Immunogold labeling and EM analysis confirmed staining of the budding compartment with HCMV gB, Rab 3, and mannosidase II in HL 411 cells. However, the markers Rab 1, Rab 2, Rab 7, Lamp 1 (late endosomes and lysosomes), and Lamp 2 (lysosomes) neither showed specific staining of the budding compartment in the immunogold labeling experiments nor colocalized with gB in the immunofluorescent colocalization experiments in any cell type studied. Together, these results suggest that the final envelopment of HCMV particles takes place mainly into a Golgi-derived secretory vacuole destined for the plasma membrane, which may release new infectious virus particles by fusion with the plasma membrane.
Critical Race Theory and Education: Racism and anti-racism in educational theory and praxis

What is Critical Race Theory (CRT) and what does it offer educational researchers and practitioners outside the US? This paper addresses these questions by examining the recent history of anti-racist research and policy in the UK. In particular, the paper argues that conventional forms of anti-racism have proven unable to keep pace with the development of increasingly racist and exclusionary education polices that operate beneath a veneer of professed tolerance and diversity. In particular, contemporary anti-racism lacks clear statements of principle and theory that risk reinventing the wheel with each new study; it is increasingly reduced to a meaningless slogan; and it risks appropriation within a reformist “can do” perspective dominated by the de-politicized and managerialist language of school effectiveness and improvement. In contrast, CRT offers a genuinely radical and coherent set of approaches that could revitalize critical research in education across a range of inquiries, not only in self-consciously “multicultural” studies. The paper reviews the developing terrain of CRT in education, identifying its key defining elements and the conceptual tools that characterize the work. CRT in education is a fast-changing and incomplete project but it can no longer be ignored by the academy beyond North America.
Dependable filtering: Philosophy and realizations

Digital content production and distribution has radically changed our business models. An unprecedented volume of supply is now on offer, whetted by the demand of millions of users from all over the world. Since users cannot be expected to browse through millions of different items to find what they might like, filtering has become a popular technique to connect supply and demand: trusted users are first identified, and their opinions are then used to create recommendations. In this domain, users' trustworthiness has been measured according to one of the following two criteria: taste similarity (i.e., “I trust those who agree with me”), or social ties (i.e., “I trust my friends, and the people that my friends trust”). The former criterion aims at identifying concordant users, but is subject to abuse by malicious behaviors. The latter aims at detecting well-intentioned users, but fails to capture the natural subjectivity of tastes. In this article, we propose a new definition of trusted recommenders, addressing those users that are both well-intentioned and concordant. Based on this characterisation, we propose a novel approach to information filtering that we call dependable filtering. We describe alternative algorithms realizing this approach, and demonstrate, by means of extensive performance evaluation on a variety of real large-scale datasets, the high degree of both accuracy and robustness they entail.
[Book review] anthropology as cultural critique, an experimental moment in the human sciences

Using cultural anthropology to analyze debates that reverberate throughout the human sciences, George E. Marcus and Michael M. J. Fischer look closely at cultural anthropology's past accomplishments, its current predicaments, its future direction, and the insights it has to offer other fields of study. The result is a provocative work that is important for scholars interested in a critical approach to social science, art, literature, and history, as well as anthropology. This second edition considers new challenges to the field which have arisen since the book's original publication.