Miracle-dz/UniFAR-FTUs huggingface dataset:
Unravelling the Diversity of Grapevine Microbiome

Vitis vinifera is one of the most widely cultivated fruit crops with a great economic impact on the global industry. As a plant, it is naturally colonised by a wide variety of both prokaryotic and eukaryotic microorganisms that interact with grapevine, having either beneficial or phytopathogenic effects, who play a major role in fruit yield, grape quality and, ultimately, in the evolution of grape fermentation and wine production. Therefore, the objective of this study was to extensively characterize the natural microbiome of grapevine. Considering that the majority of microorganisms are uncultivable, we have deeply studied the microflora of grapevine leaves using massive parallel rDNA sequencing, along its vegetative cycle. Among eukaryotic population the most abundant microorganisms belonged to the early diverging fungi lineages and Ascomycota phylum, whereas the Basidiomycota were the least abundant. Regarding prokaryotes, a high diversity of Proteobacteria, Firmicutes and Actinobacteria was unveiled. Indeed, the microbial communities present in the vineyard during its vegetative cycle were shown to be highly structured and dynamic. In all cases, the major abundant microorganisms were the yeast-like fungus Aureobasidium and the prokaryotic Enterobacteriaceae. Herein, we report the first complete microbiome landscape of the vineyard, through a metagenomic approach, and highlight the analysis of the microbial interactions within the vineyard and its importance for the equilibrium of the microecosystem of grapevines.
Optimal and System Myopic Policies for Multi-Echelon Production/Inventory Assembly Systems

In this paper optimal and near optimal policies are proposed for multi-echelon production/inventory assembly systems under continuous review with constant demand over an infinite planning horizon. Costs at each stage consist of a fixed charge per order or production setup plus a linear holding cost on "echelon" inventory. The objective is minimization of average cost per unit time. The major results of this paper are: a mathematically simple, often optimal, "system myopic" solution, a lower bound on the closeness to optimality of this solution, and a branch and bound algorithm which usually finds the optimal solution quickly.
Two systems of giant axon terminals in the cat medial geniculate body: Convergence of cortical and GABAergic inputs

The thalamus plays a critical role in processing sensory information that involves interactions between extrinsic connections and intrinsic circuitry. Little is known regarding how these different systems might interact. We found an unexpected nuclear convergence of two types of giant axon terminals, each of which must have independent origins, in the dorsal division of the cat medial geniculate body. The first class of giant terminal was labeled after injections of biotinylated dextran amines (BDA) in seven auditory cortical areas. A second type was found in sections immunostained for γ‐aminobutyric acid (GABA); these endings had the same nuclear distribution, and they were numerous. The origin of this GABAergic terminal is unknown. The giant corticothalamic terminals were presumably those described in prior accounts using different tracers (Rouiller and de Ribaupierre [1990] Neurosci. Lett. 208:29–35; Ojima [1994] Cerebral Cortex 6:646–663), but with BDA they are labeled more fully. Clusters of such endings were often linked, and hundreds may occur in a single section. Their boutons formed a substantial proportion of the corticothalamic population. Other types of corticogeniculate axon terminals were also labeled, including two kinds that are much smaller and that match closely the classical descriptions of corticothalamic axons. The giant GABAergic endings were found in all dorsal division nuclei and in thalamic visual nuclei such as the lateral posterior nucleus. Like the giant cortical endings, the giant GABAergic terminals often encircled large, pale, immunonegative profiles that may be dendritic. This implies a close spatial, and perhaps a close functional, relationship between the populations of giant axon terminals. Insofar as physiological studies found that pharmacological inactivation of rat somatic sensory cortex suppresses peripheral information transmission through the posterior thalamus, corticofugal input may be essential for normal processing (Diamond et al. [1992] J. Comp. Neurol. 319:66–84). Our findings suggest that the giant corticothalamic endings could play an important role in descending control. Perhaps they are counterbalanced by a GABAergic system and affect thalamic oscillations implicated in shifts in vigilance and attention. J. Comp. Neurol. 413:181–197, 1999. © 1999 Wiley‐Liss, Inc.
MENYIBAK CITRA PEREMPUAN DALAM CERPEN “MARIA “ ( SEBUAH KAJIAN SASTRA FEMINISME )

The title of this research is The image of women Maria’short story. The purpose of this paper is to explain how the short story writer describes the strugle of the women named Maria who struggles against gender injustice. This paper is research model that use literary review research model to collect the dataThe paper shows that The woman named Maria who became the rule main in this story is described as a strong women who always struggle to show that the women also have the power to live independenly without depen on man.In this short story maria is described as women who has interesting personality eventhough phsphysicallynot is described not so pretty. Maria has adult thinking, independent, strong principle, brave, loyal friend. Maria hates the view that the womenis is weak creature. Maria is a women who dare to fight the male domination. Maria is a women who are very loyal to husband
Dynamic output feedback sliding mode control for nonlinear systems with mismatched uncertainty

In this paper, the robust stabilization problem for a class of nonlinear uncertain systems is studied using sliding mode techniques. Matched and mismatched uncertainties are both considered. By employing the sliding surface proposed by Zak and Hui [7], the stability of the sliding mode is shown first. Then, an asymptotic observer is established to estimate the system state variables based on a constrained Lyapunov equation, and a variable structure controller is proposed to stabilize the system by exploiting the estimated state and system output. The two major limitations of [7] are eliminated. Finally a numerical example is presented to show the approach in detail.
Field programmable gate array based control of Dual Active Bridge DC/DC Converter for the UNIFLEX-PM project

This paper presents a Field Programmable Gate Array (FPGA) implemented control system for a Dual Active Bridge (DAB) DC/DC Converter. This implementation was required during the UNIFLEX-PM project to reduce the required computational burden on the Digital Signal Processor (DSP) used for the grid power flow control algorithms. The presented design is verified using one of the 25kVA Dual Active Bridge DC/DC Converters from the UNIFLEX-PM experimental prototype.
BRIDGING THE GAP BETWEEN L2 SPEECH PERCEPTION RESEARCH AND PHONOLOGICAL THEORY

A series of experiments shows that Spanish learners of English acquire the ship-sheep contrast in a way specific to their target dialect (Scottish or Southern British English) and that many learners exhibit a perceptual strategy found in neither Spanish nor English. To account for these facts as well as for the findings of earlier research on second language (L2) speech perception, we provide an Optimality Theoretic model of phonological categorization that comes with a formal learning algorithm for its acquisition. Within this model, the dialect-dependent and L2-specific facts provide evidence for the hypotheses of Full Transfer and Full Access.We would like to thank the audiences of the 25th Penn Linguistics Colloquium (Philadelphia, 2001), Generative Approaches to Language Acquisition 2001 (Palmela), and EuroSLA 11 (Paderborn, 2001) and of talks in Lisbon, Amsterdam, Edinburgh, Utrecht, and Munich, all in 2001 as well, for their questions and remarks, and Rachel Hayes, Michael Sharwood Smith, and five anonymous SSLA reviewers for their helpful comments on earlier versions of this paper. This research was partially sponsored by grant 355-75-003 to Boersma from the Netherlands Organization for Scientific Research (NWO).
Structural biology and bioinformatics in drug design: opportunities and challenges for target identification and lead discovery

Impressive progress in genome sequencing, protein expression and high-throughput crystallography and NMR has radically transformed the opportunities to use protein three-dimensional structures to accelerate drug discovery, but the quantity and complexity of the data have ensured a central place for informatics. Structural biology and bioinformatics have assisted in lead optimization and target identification where they have well established roles; they can now contribute to lead discovery, exploiting high-throughput methods of structure determination that provide powerful approaches to screening of fragment binding.
Exposed Subcutaneous Implantable Devices: An Operative Protocol for Management and Salvage

Background: Implantable venous and electrical devices are prone to exposure and infection. Indications for management are controversial, but—especially if infected—exposed devices are often removed and an additional operation is needed to replace the device, causing a delay in chemotherapy and prolonging healing time. We present our protocol for device salvage, on which limited literature is available. Methods: Between 2007 and 2013, 17 patients were treated (12 venous access ports, 3 cardiac pacemakers, and 2 subcutaneous neural stimulators). Most patients were operated within 7 days from exposure. All patients received only a single perioperative dose of prophylactic antibiotic. In cases of gross infection (n = 1), the device was immediately replaced. In the absence of clinical signs of infection:Complete capsulectomy and aggressive cleaning with an n-acetylcysteine solution and saline solution.Primary exposure of venous ports with sufficient skin coverage (n = 10): the device was covered with local skin flaps.Recurrent cases, cases with insufficient skin coverage or big devices (n = 7): the device was moved to a subpectoral pocket.Mean follow-up was 19 months. Results: Sixteen devices were saved. Only one grossly infected pacemaker was removed and replaced immediately. Only in 1 case, exposure of a venous port recurred after 18 months and was successfully moved to a subpectoral pocket. Chemotherapy was always restarted as scheduled and electrical devices remained functional. Conclusions: This protocol allows—with a straightforward operation and simple measures—to save exposed devices even several days after exposure. Submuscular placement or immediate replacement is indicated only in selected cases.
How Do Prior Ties Affect Learning by Hiring?

Research has shown that hiring R&D scientists from competitors fosters organizational learning. We examine whether hiring scientists who have many collaborative ties with the hiring firm prior to the mobility event produces different learning outcomes than hiring scientists who have few or no such ties. We theorize that prior ties reduce explorative learning and increase exploitative learning. Namely, we posit that prior ties lead the hiring firm to focus on that part of a new hire’s knowledge with which they are already familiar and that they help appropriate the new hire’s newly generated knowledge. At the same time, prior ties induce new hires to search locally within the hiring firm’s knowledge base and to produce more incremental, lower-impact innovations. Using data on R&D scientists’ mobility in the Electronics and Electrical Goods industry, we find broad support for our hypotheses. Our results extend our theoretical understanding of learning-by-hiring processes and bear practical managerial implications.
Awake Hippocampal Sharp-Wave Ripples Support Spatial Memory

Spatial Memory Perturbation The hippocampus is important for learning and memory. However, it is not clear which patterns of neural activity in the hippocampus support specific mnemonic functions. Jadhav et al. (p. 1454, published online 3 May) developed a real-time analysis system to detect and selectively interrupt a certain type of hippocampal neuronal network event—sharp-wave ripples—during learning. In awake animals, loss of sharp-wave ripples and associated memory replay activity caused a learning deficit specific to spatial working memory but had no effect on reference memory. This learning deficit was present despite the preservation of place-field representations and replay activity during rest. The neuronal “replay” of past experience may allow animals to retrieve specific memories and use them to guide behavior. The hippocampus is critical for spatial learning and memory. Hippocampal neurons in awake animals exhibit place field activity that encodes current location, as well as sharp-wave ripple (SWR) activity during which representations based on past experiences are often replayed. The relationship between these patterns of activity and the memory functions of the hippocampus is poorly understood. We interrupted awake SWRs in animals learning a spatial alternation task. We observed a specific learning and performance deficit that persisted throughout training. This deficit was associated with awake SWR activity, as SWR interruption left place field activity and post-experience SWR reactivation intact. These results provide a link between awake SWRs and hippocampal memory processes, which suggests that awake replay of memory-related information during SWRs supports learning and memory-guided decision-making.
Global stability analysis of two-dimensional LTI switched systems with saddle points

This paper investigates globally asymptotical stability issues of two-dimensional linear time-invariant (LTI) switched systems with saddle points. Without resort to any general stability analyzing techniques or ideas based on any type of Lyapunov functions, this paper proposes a globally asymptotical stability (GAS) criterion for such kind of switched systems under arbitrary periodical/quasi-periodical switchings via the classical Lyapunov stability concept. A numerical example and its simulations illustrate the new stability result is correct and effective.
Using Peer Review with Greek EFL College Students

Peer review fosters student critical thinking and self-evaluation (Wood and Kurzel 2008). Numerous studies show that peer review is effective in improving student writing (Althauser and Darnall 2001, Bean 2011), and that it benefits the students receiving as well as those giving the feedback (van den Berg, Admiraal and Pilot 2006). However, these issues have not been greatly researched in Greece. Greek culture bestows great authority to the teacher and students are not accustomed to peer feedback. I have embarked on a small-scale, exploratory, classroom-based study conducted at Deree - The American College of Greece where English is the medium of instruction. Data include first and revised drafts of three academic writing assignments, written peer comments, and learner reflections on the peer reviewing experience. To further explore student attitudes toward peer review, I also administered an online questionnaire. Initial quantitative and qualitative analyses reveal (a) in general student reviewers and reviewees alike accept peer review as an appropriate pedagogical activity; (b) students revise their writing taking into account peer feedback and (c) as reviewers, students were not more critical in giving feedback when doing peer review anonymously. Preliminary results are interpreted with an understanding of the limitations of the ongoing study.
The Functional, Social and Economic Impact of Acute Encephalitis Syndrome in Nepal – a Longitudinal Follow-Up Study

Background Over 133,000 children present to hospitals with Acute Encephalitis Syndrome (AES) annually in Asia. Japanese encephalitis (JE) accounts for approximately one-quarter of cases; in most cases no pathogen is identified and management is supportive. Although JE is known to result in neurological impairment, few studies have examined the wider impact of JE and AES on patients and their families. Methodology/Principal Findings Children (aged 1 month–14 years) with AES were assessed 5–12 months after discharge from two Nepali hospitals. Assessment included clinical examination, the Liverpool Outcome Score (LOS) - a validated assessment of function following encephalitis, questionnaires about the child's social participation since discharge, and out-of-pocket costs to the family. Children were classified as JE or ‘other AES’ based on anti-JE virus antibody titres during acute illness. Contact was made with the families of 76% (73/96) of AES children. Six children had died and one declined participation. 48% (32/66) reported functional impairment at follow-up, most frequently affecting behaviour, language or limb use. Impairment was more frequent in JE compared to ‘other AES’ cases (68% [13/19] versus 40% [19/47]; p = 0.06). 49% (26/53) had improvement in LOS between discharge and follow-up. The median out-of-pocket cost to families, including medical bills, medication and lost earnings was US$ 1151 (10 times their median monthly income) for children with severe/moderate impairment and $524 (4.6 times their income) for those with mild/no impairment (P = 0.007). Acute admission accounted for 74% of costs. Social participation was limited in 21% of children (n = 14). Conclusions/Significance Prolonged functional impairment was common following AES. Economic impact to families was substantial. Encouragingly, almost half the children improved after discharge and most reported sustained social participation. This study highlights a need for long-term medical support following AES. Rationalisation of initial expensive hospital treatments may be warranted, especially since only supportive treatment is available.
New Insight into Secreted Ribonuclease Structure: Binase Is a Natural Dimer

The biological effects of ribonucleases (RNases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. One of the most intriguing properties of RNases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient RNA hydrolysis and the evasion of ribonuclease inhibitor. Dimeric forms of microbial RNase binase secreted by Bacillus pumilus (former B. intermedius) have only been found in crystals to date. Our study is the first report directly confirming the existence of binase dimers in solution and under natural conditions of enzyme biosynthesis and secretion by bacilli. Using different variants of gel electrophoresis, immunoblotting, size-exclusion chromatography and mass-spectrometry, we revealed that binase is a stable natural dimer with high catalytic activity.
Commuting in Belgian metropolitan areas: The power of the Alonso-Muth model

In order to understand patterns of urban commuter flows, insight is required into urban spatial structure (and vice versa). The present contribution first provides a concise overview of the theoretical perspectives from which economists and geographers approach commuting issues. Subsequently, the focus shifts to the classical spatial-economic urban models and how they explain commuter movements. We conduct a number of cluster analyses from which we are able to derive a commuting typology of city region areas. We conclude that distance (which also comprises journey time and proximity of traffic infrastructure), housing characteristics, housing environment, and income continue to play key roles in commuting patterns in the metropolitan areas under consideration.
Verifying CTL* Properties of GOLOG Programs over Local-Effect Actions

GOLOG is a high-level action programming language for controlling autonomous agents such as mobile robots. It is defined on top of a logic-based action theory expressed in the Situation Calculus. Before a program is deployed onto an actual robot and executed in the physical world, it is desirable, if not crucial, to verify that it meets certain requirements (typically expressed through temporal formulas) and thus indeed exhibits the desired behaviour. However, due to the high (first-order) expressiveness of the language, the corresponding verification problem is in general undecidable. In this paper, we extend earlier results to identify a large, non-trivial fragment of the formalism where verification is decidable. In particular, we consider properties expressed in a first-order variant of the branching-time temporal logic CTL*. Decidability is obtained by (1) resorting to the decidable first-order fragment C2 as underlying base logic, (2) using a fragment of GOLOG with ground actions only, and (3) requiring the action theory to only admit local effects.
It’s all about the money: an exploration of probation fees

ABSTRACT The goal of this research was to explore how the probation requirement to pay supervision fees affects the experiences of probation. Using qualitative methods, I reveal how probation fees affected probationers’ lives in and out of probation and how these experiences may undermine probation goals. Forty-six individuals on probation were interviewed or participated in focus groups. Findings demonstrate how the probation fees were a source of constant stress that led to probation extensions, additional sanctions, and fears of probation being revoked for nonpayment. The findings highlight the pressure on probationers and focus on ways in which this probation condition may overshadow other probation goals.
Crisis Spillovers in Emerging Market Economies: Interlinkages, Vulnerabilities and Investor Behaviour

Many emerging market economy (EME) financial crises in the 1990s quickly spread to other countries. By contrast, spillovers from the Argentina crisis in 2001-02 appear to have been much more limited. Why do some crises spread widely and others do not? In this paper the joint importance of intra-EME linkages, related country-specific vulnerabilities and investor behaviour are stressed. This framework provides insights into some potential reasons behind the differing extent of spillovers in two case studies - Asia 1997-98 and Argentina 2001-02. It also highlights the need for further analysis of the less easily measurable elements of the framework, in particular changes in investor behaviour.
Improving outcomes of refractory celiac disease – current and emerging treatment strategies

Intestinal inflammation and symptoms of celiac disease (CD) usually respond well to gluten withdrawal, but rare cases are refractory to diet. Two types of refractory CD are discriminated on the basis of the presence or absence of an atypical population of mucosal lymphocytes that may progress to enteropathy-associated T-cell lymphoma. Challenges remain in the secure diagnosis of both types of refractory disease, and evidence on which to base treatment recommendations is flawed by the small numbers of reported patients and the use of different diagnostic strategies. Recent advances in our understanding of the mechanisms of the condition in conjunction with the development of immunomodulatory agents for managing other inflammatory diseases are helping to shape future approaches to targeted therapy. Progression will depend on collaboration and recruitment to trials. In the meantime, there is evidence to suggest that earlier diagnosis and better follow-up and management of CD may prevent the development of refractoriness.