Miracle-dz/UniFAR-FTUs huggingface dataset:
Distinct Roles of Estrogen Receptors &agr; and &bgr; Mediating Acute Vasodilation of Epicardial Coronary Arteries

This study investigated the contribution of estrogen receptors (ERs) &agr; and &bgr; for epicardial coronary artery function, vascular NO bioactivity, and superoxide (O2−) formation. Porcine coronary rings were suspended in organ chambers and precontracted with prostaglandin F2&agr; to determine direct effects of the selective ER agonists 4,4′,4″-(4-propyl-[1H]pyrazole-1,3,5-triyl)tris-phenol (PPT) or 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN) or the nonselective ER agonist 17&bgr;-estradiol. Indirect effects on contractility to U46619 and relaxation to bradykinin were assessed and effects on NO, nitrite, and O2− formation were measured in cultured cells. Within 5 minutes, selective ER&agr; activation by PPT, but not 17&bgr;-estradiol or the ER&bgr; agonist DPN, caused rapid, NO-dependent, and endothelium-dependent relaxation (49±5%; P<0.001 versus ethanol). PPT also caused sustained endothelium- and NO-independent vasodilation similar to 17&bgr;-estradiol after 60 minutes (72±3%; P<0.001 versus ethanol). DPN induced endothelium-dependent NO-independent relaxation via endothelium-dependent hyperpolarization (40±4%; P<0.01 versus ethanol). 17&bgr;-Estradiol and PPT, but not DPN, attenuated the responses to U46619 and bradykinin. All of the ER agonists increased NO and nitrite formation in vascular endothelial but not smooth muscle cells and attenuated vascular smooth muscle cell O2− formation (P<0.001). ER&agr; activation had the most potent effects on both nitrite formation and inhibiting O2− (P<0.05). These data demonstrate novel and differential mechanisms by which ER&agr; and ER&bgr; activation control coronary artery vasoreactivity in males and females and regulate vascular NO and O2− formation. The findings indicate that coronary vascular effects of sex hormones differ with regard to affinity to ER&agr; and ER&bgr;, which will contribute to beneficial and adverse effects of hormone replacement therapy.
Concurrent evolution of regions of the envelope and polymerase genes of human immunodeficiency virus type 1 observed during zidovudine (AZT) therapy

Nucleotide sequences of regions of the envelope (env) and polymerase (pol) genes of human immunodeficiency virus type 1 (HIV-1) proviral DNA were obtained from sequential blood and autopsy samples from an AIDS patient who had been treated with zidovudine for 9 months. Phylogenetic analyses showed that a reduction in genetic heterogeneity of the env regions of viruses present in the proviral blood population occurred during therapy, and this coincided with an increased pol gene heterogeneity. Differences were observed in different organs obtained post mortem for both the env and pol coding regions. The cardiac blood proviral population consisted mainly of variants which possessed sequences containing mutations at position 215 of the pol gene, associated with drug resistance. By contrast, the brain population consisted entirely of zidovudine sensitive genotypes, and this organ also harboured variants with genetically distinct env sequences. The lymph tissues obtained after death held more diverse proviral env and pol populations, containing both zidovudine sensitive and resistant genotypes.
Fragmentation Impairs the Microclimate Buffering Effect of Tropical Forests

Background Tropical forest species are among the most sensitive to changing climatic conditions, and the forest they inhabit helps to buffer their microclimate from the variable climatic conditions outside the forest. However, habitat fragmentation and edge effects exposes vegetation to outside microclimatic conditions, thereby reducing the ability of the forest to buffer climatic variation. In this paper, we ask what proportion of forest in a fragmented ecosystem is impacted by altered microclimate conditions driven by edge effects, and extrapolate these results to the whole Atlantic Forest biome, one of the most disturbed biodiversity hotspots. To address these questions, we collected above and below ground temperature for a full year using temperature sensors placed in forest fragments of different sizes, and at different distances from the forest edge. Principal Findings In the Atlantic forests of Brazil, we found that the buffering effect of forests reduced maximum outside temperatures by one third or more at ground level within a forest, with the buffering effect being stronger below-ground than one metre above-ground. The temperature buffering effect of forests was, however, reduced near forest edges with the edge effect extending up to 20 m inside the forest. The heavily fragmented nature of the Brazilian Atlantic forest means that 12% of the remaining biome experiences altered microclimate conditions. Conclusions Our results add further information about the extent of edge effects in the Atlantic Forest, and we suggest that maintaining a low perimeter-to-area ratio may be a judicious method for minimizing the amount of forest area that experiences altered microclimatic conditions in this ecosystem.
Accounting Conservatism and Transitory Earnings in Value and Growth Strategies

The value premium earned on value and growth investment strategies increases after adjusting for transitory earnings and for the accounting conservatism bias in the book value of equity. Simple investment strategies based on earnings-to-price (E/P) and book-to-market (B/M) performed on the Swedish stock market between 1980 and 2004 generate an annual value premium of 11 to 14%. Adjustments for transitory earnings and for the conservatism bias increase the value premium by 2 to 4 percentage points, and at the same time they improve the consistency of earning it. These results suggest that transitory earnings and accounting conservatism introduce noise into E/P and B/M measures. Adjusting for these accounting characteristics makes the identification based on E/P and B/M more effective. Copyright (c) 2010 Blackwell Publishing Ltd.
Contextual and Structural Representations of Market-mediated Economic Value

How do we assign value to economic transactions? To answer this question, we must consider whether the value of objects is inherent, is a product of social interaction, or involves other mechanisms. Economic theory predicts that there is an optimal price for any market transaction, and can be observed during auctions or other bidding processes. However, there are also social, cultural, and cognitive components to the assignation of value, which can be observed in both human and non-human Primate societies. While behaviors related to these factors are embedded in market interactions, they also involve a biological substrate for the assignation of value (valuation). To synthesize this diversity of perspectives, we will propose that the process of valuation can be modeled computationally and conceived of as a set of interrelated cultural evolutionary, cognitive, and neural processes. To do this, contextual geometric structures (CGS) will be placed in an agent-based context (minimal and compositional markets). Objects in the form of computational propositions can be acquired and exchanged, which will determine the value of both singletons and linked propositions. Expected results of this model will be evaluated in terms of their contribution to understanding human economic phenomena. The paper will focus on computational representations and how they correspond to real-world concepts. The implications for evolutionary economics and our contemporary understanding of valuation and market dynamics will also be discussed.
Fault severity recognition of hydraulic piston pumps based on EMD and feature energy entropy

Based on Empirical Mode Decomposition (EMD) and feature energy entropy, a method for the fault severity recognition of piston pumps is proposed in this paper. The discharge pressure signals of piston pumps are decomposed into a series of Intrinsic Mode Function (IMF) components by using EMD. Then, some useful IMF components are selected by calculating correlation coefficient between the signal reconstructed by the selected IMFs and the original signal. The characteristic vector is constructed by computing the normalized energy of every selected IMF, and the feature energy entropy can be obtained. The experimental results indicate that the proposed method can recognize the fault severity of pumps effectively.
Host specificity of two pollinating seed‐consuming fly species is not related to soil moisture of host plant in the high Himalayas

Abstract Studying the drivers of host specificity can contribute to our understanding of the origin and evolution of obligate pollination mutualisms. The preference–performance hypothesis predicts that host plant choice of female insects is related mainly to the performance of their offspring. Soil moisture is thought to be particularly important for the survival of larvae and pupae that inhabit soil. In the high Himalayas, Rheum nobile and R. alexandrae differ in their distribution in terms of soil moisture; that is, R. nobile typically occurs in scree with well‐drained soils, R. alexandrae in wetlands. The two plant species are pollinated by their respective mutualistic seed‐consuming flies, Bradysia sp1. and Bradysia sp2. We investigated whether soil moisture is important for regulating host specificity by comparing pupation and adult emergence of the two fly species using field and laboratory experiments. Laboratory experiments revealed soil moisture did have significant effects on larval and pupal performances in both fly species, but the two fly species had similar optimal soil moisture requirements for pupation and adult emergence. Moreover, a field reciprocal transfer experiment showed that there was no significant difference in adult emergence for both fly species between their native and non‐native habitats. Nevertheless, Bradysia sp1. , associated with R. nobile, was more tolerant to drought stress, while Bradysia sp2. , associated with R. alexandrae, was more tolerant to flooding stress. These results indicate that soil moisture is unlikely to play a determining role in regulating host specificity of the two fly species. However, their pupation and adult emergence in response to extremely wet or dry soils are habitat‐specific.
Trust Games Measure Trust

The relationship between trust and risk is a topic of enduring interest. Although there are substantial differences between the ideas the terms express, many researchers from different disciplines have pointed out that these two concepts become very closely related in personal exchange contexts. This raises the important practical concern over whether behaviors in the widely-used “trust game” actually measure trust, or instead reveal more about risk attitudes. It is critical to confront this question rigorously, as data from these games are increasingly used to support conclusions from a wide variety of fields including macroeconomic development, social psychology and cultural anthropology. The aim of this paper is to provide cogent evidence on the relationship between trust and risk in “trust” games. Subjects in our experiment participate either in a trust game or in its risk game counterpart. In the trust version, subjects play a standard trust game and know their counterparts are human. In the risk version, subjects know their counterparts are computers making random decisions. We compare decisions between these treatments, and also correlate behavior with subjects’ risk attitudes as measured by the Holt and Laury (2002) risk instrument. We provide evidence that trusting behavior is different than behavior under risk. In particular, (i) decisions patterns in our trust and risk games are significantly different; and (ii) risk attitudes predict decisions in the risk game, but not the trust game.
Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil.

INTRODUCTION Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). METHODOLOGY We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. RESULTS ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECII-positive; and two ECI strains from a human case (1982) and from bovine meat (2009). CONCLUSIONS The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.
Wogonin Suppresses Melanoma Cell B16-F10 Invasion and Migration by Inhibiting Ras-Medicated Pathways

The patients diagnosed with melanoma have a bad prognosis for early regional invasion and distant metastases. Wogonin (5,7-dihydroxy-8-methoxyflavone) is one of the active components of flavonoids that extracts from Scutellariae radix. Several previous studies reported that wogonin possesses antitumor effect against leukemia, gastrointestinal cancer and breast cancer. In this study, we used melanoma cell B16-F10 to further investigate the anti-invasive and anti-migratory activity of wogonin. Our date showed that wogonin caused suppression of cell migration, adhesion, invasion and actin remodeling by inhibiting the expression of matrix metalloproteinase-2 and Rac1 in vitro. Wogonin also reduced the number of the tumor nodules on the whole surface of the lung in vivo. Furthermore, the examination of mechanism revealed that wogonin inhibited Extracellular Regulated protein Kinases and Protein Kinase B pathways, which are both medicated by Ras. Insulin-like growth factor-1-induced or tumor necrosis factor-α-induced invasion was also inhibited by wogonin. Therefore, the inhibitory mechanism of melanoma cell invasion by wogonin might be elucidated.
Cutaneous Mechanoreceptor Feedback from the Hand and Foot Can Modulate Muscle Sympathetic Nerve Activity

Stimulation of high threshold mechanical nociceptors on the skin can modulate efferent sympathetic outflow. Whether low threshold mechanoreceptors from glabrous skin are similarly capable of modulating autonomic outflow is unclear. Therefore, the purpose of this study was to examine the effects of cutaneous afferent feedback from the hand palm and foot sole on efferent muscle sympathetic nerve activity (MSNA). Fifteen healthy young participants (9 male; 25 ± 3 years [range: 22–29]) underwent microneurographic recording of multi-unit MSNA from the right fibular nerve during 2 min of baseline and 2 min of mechanical vibration (150 Hz, 220 μm peak-to-peak) applied to the left hand or foot. Each participant completed three trials of both hand and foot stimulation, each separated by 5 min. MSNA burst frequency decreased similarly during the 2 min of both hand (20.8 ± 8.9 vs. 19.3 ± 8.6 bursts/minute [Δ −8%], p = 0.035) and foot (21.0 ± 8.3 vs. 19.5 ± 8.3 bursts/minute [Δ −8%], p = 0.048) vibration but did not alter normalized mean burst amplitude or area (All p > 0.05). Larger reductions in burst frequency were observed during the first 10 s (onset) of both hand (20.8 ± 8.9 vs. 17.0 ± 10.4 [Δ −25%], p < 0.001) and foot (21.0 ± 8.3 vs. 18.3 ± 9.4 [Δ −16%], p = 0.035) vibration, in parallel with decreases in normalized mean burst amplitude (hand: 0.45 ± 0.06 vs. 0.36 ± 0.14% [Δ −19%], p = 0.03; foot: 0.47 ± 0.07 vs. 0.34 ± 0.19% [Δ −27%], p = 0.02) and normalized mean burst area (hand: 0.42 ± 0.05 vs. 0.32 ± 0.12% [Δ −25%], p = 0.003; foot: 0.47 ± 0.05 vs. 0.34 ± 0.16% [Δ −28%], p = 0.01). These results demonstrate that tactile feedback from the hands and feet can influence efferent sympathetic outflow to skeletal muscle.
Micropruning: the mechanism of turning of Aplysia growth cones at substrate borders in vitro

Growth cones of Aplysia californica neurons were observed with video- enhanced contrast-differential interference contrast (VEC-DIC) microscopy as they turned at a border between poly-L-lysine-treated and untreated glass. Growth cones that turned generally developed 2 distinct active areas of filopodial and veil formation, much in the way of growth cones undergoing branching. Both active areas advanced, but turning of the neurite occurred through the selective resorption of the incipient branches developing on the untreated substrate. Thus, micropruning of developing regions of the growth cone, rather than the asymmetric extension of filopodia or veils, was primarily responsible for directing neurite growth. We present the hypothesis that abrupt turns by growing neurites are mediated by 2 sets of signals, one causing growth cone splitting, and a second set regulating the survival of the separate branches.
Chinese State-Owned Enterprises: Are They Inefficient?

Abstract Using a panel data set of 200,000+ Chinese firms constructed by merging the Chinese census of manufacturing firms for 2000–2005, we compare the performance of Chinese state-owned enterprises (SOEs) and private firms in terms of rates of return, productivity, growth, costs, and investment. Using panel regressions, we find that Chinese industrial state-owned enterprises are, indeed, less efficient than private firms and pay less attention to costs, inventories, accounts receivables, investment, employee welfare, financing, and administration. We find that this adversely affects their performance. The findings are consistent with the soft-budget constraint hypothesis.
Lack of Genetic Influence on the Innate Inflammatory Response to Helicobacter Infection of the Gastric Mucosa

Helicobacter pylori (H. pylori) is a bacterial pathogen that resides at the gastric mucosa and has a world-wide prevalence of over 50%. Infection usually lasts for the life of the host, and although all infected individuals will develop histologic gastritis only a subset will develop symptomatic gastritis, peptic ulcer disease, gastric MALT lymphoma, or gastric adenocarcinoma. The bacterial and host factors that determine clinical outcome and influence the development of widely varying diseases have not been elucidated. We compared disease in Helicobacter-infected severe combined immunodeficient (SCID) mice on different genetic backgrounds with their corresponding immunocompetent partners to determine if the genetics of the host significantly impacts the innate inflammatory outcome, independent of variations in bacterial virulence factors. BALB/c SCID and C57BL/6 SCID mice developed equivalent histologic gastritis by 8 weeks of infection. Immunocompetent BALB/c mice and C57BL/6 mice developed significantly lower or higher degrees of inflammation respectively. Innate inflammation in immunodeficient mice on the C57BL/6 background remained low even in the absence of the regulatory cytokine IL-10. These results demonstrate that adaptive immunity is not required for the generation of low level inflammation in response to Helicobacter infection and that the degree of inflammation is consistent among different genetic backgrounds. Additionally, this inflammation is limited even in the absence of regulatory T cells.
Phylogeography of a post‐glacial colonizer: Microtus longicaudus (Rodentia: Muridae)

The molecular phylogeography of Microtus longicaudus was investigated with DNA sequences of the mitochondrial cytochrome b gene. We used phylogenetic and pairwise distance methods to reconstruct the history of the species with particular emphasis on the Pacific Northwest. Genetic variation across the species was consistent with vicariant events during the Pleistocene and subsequent northern postglacial expansion following the receding Laurentide and Cordilleran ice sheets. The largest break (> 6% uncorrected sequence divergence) was found to exist between populations found southeast of the Colorado River (eastern Arizona, Colorado, Wyoming and New Mexico) and all other western populations. Other well‐supported subclades were composed of samples from: (i) the islands and north coast of southeast Alaska; (ii) eastern Alaska, British Columbia, Washington and Oregon; and (iii) northern California, Idaho and Montana. Within subclades, divergence was low. Our results suggest that the close relationships among haplotypes within northern subclades are a result of recent colonization, whereas higher among‐subclade divergence is caused by genetic differentiation during prolonged periods of isolation, possibly as a result of mid‐Pleistocene climatic events.
Evidence for a schedule-dependent deleterious interaction between paclitaxel, vinblastine and cisplatin (PVC) in the treatment of advanced transitional cell carcinoma

A phase II study to evaluate the efficacy and toxicity of the combination of vinblastine, paclitaxel and cisplatin (PVC) in previously untreated patients with advanced transitional cell carcinoma. Chemotherapy naive patients with locally advanced or metastatic transitional cell carcinoma received the intravenous combination of paclitaxel 175 mg/m2 over three hours followed by cisplatin 70 mg/m2 over 3 hours on day 1 and vinblastine 3 mg/m2 as a bolus on days 1 and 8 on a 21-day cycle, to a maximum of 6 cycles. The day 8 vinblastine was omitted if the total neutrophil count was <1.0. 15 patients (13 M, 2 F) of median age 66 (54–75) received a median of 5 cycles of treatment. There were two complete responses (13%; 95% CI 2–40%) and five partial responses (33%; 95% CI 12–62%), for an overall response rate of 46% (95% CI 21–73%). Responses occurred only in those with locally recurrent tumours and/or lymph nodes involved. Neutropenia at Grade 3–4 occurred in 14 of 67 cycles (21%) resulting in 7 episodes of neutropenic sepsis. Grade 3–4 thrombocytopenia was not observed. Other Grade 3 toxicity included alopecia (10 pts), diarrhoea (2 pts), constipation resulting in bowel obstruction (2 pts), nephrotoxicity (1 pt), myalgic pain (1 pt) and peripheral neuropathy (1 pt). Six patients developed Grade 2 paraesthesia. The median time to progression was 6 months and the median survival was 11 months. The regimen PVC was both less effective against transitional cell carcinoma and less toxic than expected. This may reflect an inhibitory interaction between vinblastine and paclitaxel and this schedule cannot be recommended for further investigation. © 2000 Cancer Research Campaign http://www.bjcancer. com