Miracle-dz/UniFAR-FTUs huggingface dataset:
Attentional Modulation of Unconscious Automatic Processes: Evidence from Event-related Potentials in a Masked Priming Paradigm

Automatic processes are usually thought to occur independently of any cognitive resources. This traditional view has been recently challenged by showing that temporal attention to a target stimulus is a prerequisite for automatic response priming. The event-related potential (ERP) study reported here extends this research by pursuing a somewhat different approach. In two experiments, it was investigated whether masked semantic priming effects can be modulated by temporal attention to the prime using a cueing procedure. We hypothesized that masked priming is amplified when attention is directed to the stimulus stream in the time window of masked prime presentation, even in the absence of any prime awareness. ERPs were recorded while subjects performed a primed lexical decision task. Target words were preceded by semantically related or unrelated masked prime words, which were not consciously identified. A cue stimulus prompted subjects to direct their attention to the stimulus stream either shortly before the masked prime (short cue interval) or a long time interval before. Priming affected the amplitude of the N400 ERP component, an electrophysiological index of semantic processing. Unrelated primetarget pairs elicited a larger N400 than related pairs (N400 priming effect). Most importantly, this masked N400 priming effect was strongest when the cue interval and the stimulus onset asynchronies were short. The present results show that temporal attention to the prime is a prerequisite for obtaining masked N400 priming effects. They also demonstrate that unconscious automatic processes are susceptible to attentional modulation.
Development of an Alfalfa SNP Array and Its Use to Evaluate Patterns of Population Structure and Linkage Disequilibrium

A large set of genome-wide markers and a high-throughput genotyping platform can facilitate the genetic dissection of complex traits and accelerate molecular breeding applications. Previously, we identified about 0.9 million SNP markers by sequencing transcriptomes of 27 diverse alfalfa genotypes. From this SNP set, we developed an Illumina Infinium array containing 9,277 SNPs. Using this array, we genotyped 280 diverse alfalfa genotypes and several genotypes from related species. About 81% (7,476) of the SNPs met the criteria for quality control and showed polymorphisms. The alfalfa SNP array also showed a high level of transferability for several closely related Medicago species. Principal component analysis and model-based clustering showed clear population structure corresponding to subspecies and ploidy levels. Within cultivated tetraploid alfalfa, genotypes from dormant and nondormant cultivars were largely assigned to different clusters; genotypes from semidormant cultivars were split between the groups. The extent of linkage disequilibrium (LD) across all genotypes rapidly decayed to 26 Kbp at r2 = 0.2, but the rate varied across ploidy levels and subspecies. A high level of consistency in LD was found between and within the two subpopulations of cultivated dormant and nondormant alfalfa suggesting that genome-wide association studies (GWAS) and genomic selection (GS) could be conducted using alfalfa genotypes from throughout the fall dormancy spectrum. However, the relatively low LD levels would require a large number of markers to fully saturate the genome.
On three endemic species of the linyphiid spider genus Canariphantes Wunderlich, 1992 (Araneae, Linyphiidae) from the Azores archipelago.

We describe Canariphantes junipericola n. sp. and C. relictus n. sp. , new single-island endemic linyphiid spiders from the islands of Flores and Santa Maria (Azorean archipelago, Macaronesia), respectively. The female of the first species was incorrectly assigned to Canariphantes acoreensis (Wunderlich, 1992), a species occurring in four islands in the Central Group of Azores (Faial, Pico, São Jorge and Terceira). The latter species is transferred to Canariphantes, its male redescribed and the female genitalia described for the first time. We discuss the systematic affinities of these new species and comment on their conservation status.
e-Authentication for online assessment: A mixed-method study

Authenticating the students’ identity and authenticity of their work is increasingly important to reduce academic malpractices and for quality assurance purposes in Education. There is a growing body of research about technological innovations to combat cheating and plagiarism. However, the literature is very limited on the impact of e-authentication systems across distinctive end-users because it is not a widespread practice at the moment. A considerable gap is to understand whether the use of e-authentication systems would increase trust on e-assessment, and to extend, whether students’ acceptance would vary across gender, age and previous experiences. This study aims to shed light on this area by examining the attitudes and experiences of 328 students who used an authentication system known as adaptive trust-based e-assessment system for learning (TeSLA) . Evidence from mixed-method analysis suggests a broadly positive acceptance of these e-authentication technologies by distance education students. However, significant differences in the students’ responses indicated, for instance, that men were less concerned about providing personal data than women; middle-aged participants were more aware of the nuances of cheating and plagiarism; while younger students were more likely to reject e-authentication, considerably due to data privacy and security and students with disabilities due to concerns about their special needs.
A Fluorescence Quenching Analysis of the Binding of Fluoroquinolones to Humic Acid

Fluorescence quenching was used to investigate the interaction of six fluoroquinolones with humic acid. Static quenching was observed for the binding of ciprofloxacin, enoxacin, fleroxacin, levofloxacin, norfloxacin, and ofloxacin to humic acid. The equilibrium binding constants were found from Stern–Volmer plots of the data. The quenching experiments were repeated over a temperature range of 25–45 ℃ and van’t Hoff plots were generated. From these linear plots, thermodynamic values were calculated for ΔH, ΔG, and ΔS for each of the fluoroquinolones. The equilibrium binding constants were found to be <1 for all the antibiotics studied. The calculated ΔH values were all negative and ranged from −9.5 to −27.6 kJ/mol. The high water solubility of the antibiotics and low ΔH of binding suggests that the antibiotics will be transported easily through the environment. Finally, whether the fluoroquinolones are in a protonated, deprotonated, or partially protonated state is found to correlate to the strength of binding to humic acid.
Introducing case-based peer-assisted learning in a professional course

This paper describes students’ experience of participating in a case-based peer-assisted learning (PAL) program in order to examine whether the approach is pedagogically effective and likely to contribute to students’ professional development. It presents the findings of a study which examined the integration of PAL and case-based learning (CBL) in a multidisciplinary radiologic biology unit as part of an undergraduate degree program in Radiography and Medical Imaging. The study indicated the value of students working together in small groups to prepare and offer a case-based tutorial for their peers, with the cases offering a focus for their research and teaching in the multidisciplinary context. Working in groups and peer teaching were the most beneficial aspects of the program for enhancing student learning. The program involved three episodes of peer assessment (assessing teaching, assessing learning and assessing contributions to group work), with the first of these contributing most to the program, though further development and exploration of the latter two aspects are needed. The integration of PAL with CBL provided a ‘real world’ context for teaching and learning. Students recognised that interacting with peers would be an important aspect of their professional lives and that the program offered them opportunities to prepare for this. Implementation and evaluation of this approach with other groups of students in vocational and non-vocational courses would determine its broader pedagogical effectiveness.
Contributions of Protein-Coding and Regulatory Change to Adaptive Molecular Evolution in Murid Rodents

The contribution of regulatory versus protein change to adaptive evolution has long been controversial. In principle, the rate and strength of adaptation within functional genetic elements can be quantified on the basis of an excess of nucleotide substitutions between species compared to the neutral expectation or from effects of recent substitutions on nucleotide diversity at linked sites. Here, we infer the nature of selective forces acting in proteins, their UTRs and conserved noncoding elements (CNEs) using genome-wide patterns of diversity in wild house mice and divergence to related species. By applying an extension of the McDonald-Kreitman test, we infer that adaptive substitutions are widespread in protein-coding genes, UTRs and CNEs, and we estimate that there are at least four times as many adaptive substitutions in CNEs and UTRs as in proteins. We observe pronounced reductions in mean diversity around nonsynonymous sites (whether or not they have experienced a recent substitution). This can be explained by selection on multiple, linked CNEs and exons. We also observe substantial dips in mean diversity (after controlling for divergence) around protein-coding exons and CNEs, which can also be explained by the combined effects of many linked exons and CNEs. A model of background selection (BGS) can adequately explain the reduction in mean diversity observed around CNEs. However, BGS fails to explain the wide reductions in mean diversity surrounding exons (encompassing ∼100 Kb, on average), implying that there is a substantial role for adaptation within exons or closely linked sites. The wide dips in diversity around exons, which are hard to explain by BGS, suggest that the fitness effects of adaptive amino acid substitutions could be substantially larger than substitutions in CNEs. We conclude that although there appear to be many more adaptive noncoding changes, substitutions in proteins may dominate phenotypic evolution.
PRDM8 exhibits antitumor activities toward hepatocellular carcinoma by targeting NAP1L1

Hepatocellular carcinoma (HCC) is a major leading cause of cancer mortality worldwide. PRDI‐BF1 and RIZ homology domain containing 8 (PRDM8) is a key regulator in neural development and testis steroidogenesis; however, its role in liver carcinogenesis remains to be investigated. In this study, PRDM8 was found to be down‐regulated in HCC, which was linked with shorter recurrence‐free survival. Lentiviral‐based overexpression and knockdown approaches showed that PRDM8 inhibited HCC cell proliferation, migration, and invasion. PRDM8 caused G1/S cell cycle arrest and induced apoptosis. An in vivo tumor model confirmed the antitumor role of PRDM8 in HCC growth and metastasis. Mechanistic study showed that PRDM8 suppressed the PI3K/AKT/mTOR signaling cascade through the regulation of nucleosome assembly protein 1‐like 1 (NAP1L1). Conclusion: PRDM8 as a functional tumor suppressor is frequently down‐regulated in HCC. Through regulating NAP1L1, PRDM8 inhibits PI3K/AKT/mTOR signaling in HCC. PRDM8 is a potential target for therapies of HCC. (Hepatology 2018).
VEGETATION PATTERN FORMATION IN SEMI-ARID GRAZING SYSTEMS

Hypotheses about the origin of vegetation pattern formation in semi-arid areas around the world almost all include a common feature of semi-arid areas: the presence of a positive feedback between plant density and water infiltration. We investigate whether this positive feedback and the spatial redistribution of runoff water are sufficient to explain vegetation pattern formation. For this purpose, we analyze a spatially explicit model con- sisting of partial differential equations using a method for demonstrating pattern formation (Turing analysis). Our analysis reveals that pattern formation can occur in semi-arid areas given only the positive feedback between plant density and local water infiltration coupled with the spatial redistribution of runoff water. Thus, slope and underlying heterogeneity are not essential conditions. Other factors in the model, such as herbivory, plant dispersal, rainfall, and drought tolerance of plants, appear to determine under what conditions pattern formation is likely but are not the primary factors that generate the patterns. The model is in agreement with field observations and indicates the conditions for which vegetation pattern formation can be expected in arid and semi-arid grazing systems.
Performance Characterisation of AGR Fuel Cladding Relevant to Long-term in-Pond Storage in pH-moderated Aqueous Environment

Fuel cladding of the UK? s advanced gas-cooled reactors (AGR) is made of 20%Cr/25%Ni? Nb stabilised austenitic stainless steel. The research reported in this paper aims to enhance the mechanistic understanding of AGR fuel cladding performance. Thermal heat treatments were applied to simulate a range of cladding microstructure, including a sensitised and stabilised condition. Oxalic acid metallographic etching and Electron Back-Scattered Diffraction (EBSD) were used to characterise microstructure, augmented by electro-chemical assessments to provide a quantitative measure of the degree of sensitisation. Anodic polarisaiton tests in 500ppm chloride containing aqueous environments at neutral pH and pH 11.5 indicated differences in microstructure corrosion resistance. In neutral environment pitting corrosion is observed in as-received microstructure, inter-granular corrosion initiating at triple junctions in the sensitised microstructures, and localised pitting corrosion at Nb-rich precipitates interfaces in the stabilised microstructure. Whereas at high pH a suppression of corrosion was achieved, supporting the strategy to pH-control storage pond environments. However, microstructure analysis of the sample surfaces after exposure to pH 11.5 solution indicated dissolution of Nb-containing precipitates, which is currently subject of further investigations. The relationship between microstructure conditions, corrosion morphology, with relevance to currently applied corrosion mitigation strategies for in-pond storage of AGR fuel cladding will be elaborated.
Trauma and Autonomic Dysregulation: Episodic—Versus Systemic—Negative Affect Underlying Cardiovascular Risk in Posttraumatic Stress Disorder

Objective Posttraumatic stress disorder (PTSD) has been linked to elevated heart rate (HR) and reduced heart rate variability (HRV) in cross-sectional research. Recent evidence suggests that this link may be driven by individual differences in autonomic arousal associated with momentary negative affect (NA). Using ecological momentary assessment (EMA) of NA and minute-to-minute HR/HRV monitoring, we examined whether NA-related HR/HRV mediated the association of PTSD symptom severity with 24-hour HRV and endothelial functioning. Methods One hundred ninety-seven young adults (18–39 years), 93 with PTSD, underwent 1 day of Holter monitoring while concurrently reporting NA levels via EMA. Two noninvasive measures of endothelial functioning—flow-mediated dilation and hyperemic flow—were also collected. Multilevel modeling was used to assess the associations of momentary NA with HR and low- and high-frequency HRV during the 5-minute intervals after each EMA reading. Latent variable modeling was then used to determine whether individual differences in these associations mediated the association of PTSD symptom severity with 24-hour HRV, flow-mediated dilation, and hyperemic flow. Results PTSD symptom severity was positively associated with NA-related autonomic arousal (&bgr; = .21, p < .001), which significantly mediated the association of PTSD symptom severity with 24-hour HRV and hyperemic flow, accounting for 62% and 34% of their associations, respectively, while overshadowing the influence of smoking, lifetime alcohol dependence, sleep duration, mean NA, and episodes of acute NA. Conclusions Results suggest that NA-related autonomic arousal is both a primary factor driving cardiovascular risk in PTSD and a potential point of intervention.
Statistical Challenges in Analyses of Chamber‐Based Soil CO2 and N2O Emissions Data

CSA News 13 Measurements of soil greenhouse gas (GHG) emissions have gained a lot of recent attention in an effort to potentially increase agriculture’s role in mitigating climate effects. However, it may not be well recognized that the nature of chamber-based GHG data is such that analyses require advanced statistical techniques to fully explore experimental treatment effects. Moreover, for soil GHG data, some experimental design approaches can enhance while others can weaken an experiment’s ability to detect treatment differences.
A Relationship of Socio-Personal Factors with Job Satisfaction of Veterinary Officers in Punjab

Job satisfaction has emerged as one of the most important aspects of human resource management in recent times. The purpose of the study was to find a relationship of socio-personal factors and job satisfaction of Veterinary Officers in Punjab. Job satisfaction was operationalised as the cumulative score obtained by the respondents in relation to selected facets of the job. It was found that the veterinarians were partially satisfied with their job with a mean score of 3.49 on a scale of 1–5. Job satisfaction had a positive and significant association with experience. It was found that job satisfaction had a negative significant association with distance of place of posting. There was no significant relationship between age, gender, qualification, background and marital status of the veterinarians and the level of job satisfaction.
Local Community Detection in Dynamic Networks

Given a time-evolving network, how can we detect communities over periods of high internal and low external interactions? To address this question we generalize traditional local community detection in graphs to the setting of dynamic networks. Adopting existing static-network approaches in an “aggregated” graph of all temporal interactions is not appropriate for the problem as dynamic communities may be short-lived and thus lost when mixing interactions over long periods. Hence, dynamic community mining requires the detection of both the community nodes and an optimal time interval in which they are actively interacting. We propose a filter-and-verify framework for dynamic community detection. To scale to long intervals of graph evolution, we employ novel spectral bounds for dynamic community conductance and employ them to filter suboptimal periods in near-linear time. We also design a time-and-graph-aware locality sensitive hashing family to effectively spot promising community cores. Our method PHASR discovers communities of consistently higher quality (2 to 67 times better) than those of baselines. At the same time, our bounds allow for pruning between 55% and 95% of the search space, resulting in significant savings in running time compared to exhaustive alternatives for even modest time intervals of graph evolution.
Cambodian boys’ transitions into young adulthood: exploring the influence of societal and masculinity norms on young men’s health

Abstract A growing body of evidence focuses on the experiences of young men in low-income countries, including their health vulnerabilities. Much of this research has been conducted in sub-Saharan Africa, Asia and Latin America. Limited evidence exists on the norms influencing boys’ transitions into young adulthood in Southeast Asia, and in Cambodia in particular. To help fill this gap, we conducted a comparative case study using participatory methods with 16–19-year-old young men in rural and urban Cambodia, and with the adults who intersect in their lives. Findings suggest that in line with their counterparts growing up elsewhere, Cambodian boys welcome becoming young men as a time of newly acquired adult roles and responsibilities, while some may experience growing up as a time of bodily change, burgeoning sexual feelings and limited sources of support and guidance. As a result, some may strive more intensely to conform to the alcohol use and violence modelled by the older men in their environments. Additional research is needed to better understand the vulnerabilities of boys’ transition into young adulthood in Cambodia today, and how to prevent or reduce engagement with these more negative practices.
Opportunistic screening for heart failure with natriuretic peptides in patients with atrial fibrillation: a meta-analysis of individual participant data of four screening studies

Objective Heart failure (HF) often coexists in atrial fibrillation (AF) but is frequently unrecognised due to overlapping symptomatology. Furthermore, AF can cause elevated natriuretic peptide levels, impairing its diagnostic value for HF detection. We aimed to assess the prevalence of previously unknown HF in community-dwelling patients with AF, and to determine the diagnostic value of the amino-terminal pro B-type natriuretic peptide (NTproBNP) for HF screening in patients with AF. Methods Individual participant data from four HF-screening studies in older community-dwelling persons were combined. Presence or absence of HF was in each study established by an expert panel following the criteria of the European Society of Cardiology. We performed a two-stage patient-level meta-analysis to calculate traditional diagnostic indices. Results Of the 1941 individuals included in the four studies, 196 (10.1%) had AF at baseline. HF was uncovered in 83 (43%) of these 196 patients with AF, versus 381 (19.7%) in those without AF at baseline. Median NTproBNP levels of patients with AF with and without HF were 744 pg/mL and 211 pg/mL, respectively. At the cut-point of 125 pg/mL, sensitivity was 93%, specificity 35%, and positive and negative predictive values 51% and 86%, respectively. Only 23% of all patients with AF had an NTproBNP level below the 125 pg/mL cut-point, with still a 13% prevalence of HF in this group. Conclusions With a prevalence of nearly 50%, unrecognised HF is common among community-dwelling patients with AF. Given the high prior change, natriuretic peptides are diagnostically not helpful, and straightforward echocardiography seems to be the preferred strategy for HF screening in patients with AF.
Compound heterozygosity for loss‐of‐function FARSB variants in a patient with classic features of recessive aminoacyl‐tRNA synthetase‐related disease

Aminoacyl‐tRNA synthetases (ARSs) are ubiquitously expressed enzymes that ligate amino acids onto tRNA molecules. Genes encoding ARSs have been implicated in phenotypically diverse dominant and recessive human diseases. The charging of tRNAPHE with phenylalanine is performed by a tetrameric enzyme that contains two alpha (FARSA) and two beta (FARSB) subunits. To date, mutations in the genes encoding these subunits (FARSA and FARSB) have not been implicated in any human disease. Here, we describe a patient with a severe, lethal, multisystem, developmental phenotype who was compound heterozygous for FARSB variants: p.Thr256Met and p.His496Lysfs*14. Expression studies using fibroblasts isolated from the proband revealed a severe depletion of both FARSB and FARSA protein levels. These data indicate that the FARSB variants destabilize total phenylalanyl‐tRNA synthetase levels, thus causing a loss‐of‐function effect. Importantly, our patient shows strong phenotypic overlap with patients that have recessive diseases associated with other ARS loci; these observations strongly support the pathogenicity of the identified FARSB variants and are consistent with the essential function of phenylalanyl‐tRNA synthetase in human cells. In sum, our clinical, genetic, and functional analyses revealed the first FARSB variants associated with a human disease phenotype and expand the locus heterogeneity of ARS‐related human disease.