Miracle-dz/UniFAR-FTUs huggingface dataset:
Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain

Recent cloning of auxiliary subunits associated with voltage-gated ion channels and their subsequent coexpression with the channel forming alpha-subunits has revealed that the expression level, gating and conductance properties of the expressed channels can be profoundly affected by the presence of an auxiliary subunit polypeptide. In the present study, we raised antibodies against the beta-subunit associated with the bovine dendrotoxin sensitive K(+)-channel complex and used these antibodies to characterize the related beta-subunit polypeptides in rat brain. The anti-beta-subunit antibodies displayed a specific reaction on immunoblots of rat brain membranes with a major 38 kDa polypeptide, and a minor 41 kDa polypeptide, which correspond closely to the predicted sizes of the Kv beta 2 and Kv beta 1 beta-subunit polypeptides, respectively, recently cloned from rat brain. Reciprocal coimmunoprecipitation experiments revealed that the beta-subunit polypeptides are associated with Kv1.2 and Kv1.4, but not Kv2.1, alpha- subunits. Immunohistochemical staining revealed that the beta-subunit polypeptides were widely distributed in adult rat brain. Moreover, the cellular distribution of beta-subunit immunoreactivity corresponded closely with immunoreactivity for Kv1.2, and to a lesser extent Kv1.4, but not with Kv2.1. These results suggest that neuronal mechanisms may exist to direct the selective interaction of K+ channel alpha- and beta- subunit polypeptides, and that the properties of K+ channels in specific subcellular domains may be regulated by the formation of heteromultimeric K+ channel complexes containing specific combinations of alpha- and beta-subunits.
Mucosal mitochondrial function and antioxidant defences in patients with gastric carcinoma.

BACKGROUND Cancer cells have alterations in energy metabolism due to defective mitochondrial function. This may be due to generation of excessive free radicals and/or defective antioxidant enzyme systems. The aim of the present study was to assess mitochondrial function and antioxidant defences in the gastric mucosa of patients with gastric carcinoma (CA). METHODS Gastric mucosal mitochondrial function was assessed by means of the reduction of tetrazolium dye (MTT), and levels of antioxidants such as glutathione S-transferase (GST), catalase, superoxide dismutase (SOD), and thiols were measured in biopsy specimens taken from the tumour mucosa (TM) and tumour-free (TF) mucosa, 2 cm away from the tumour, in 49 patients with gastric CA and compared with that in 54 controls. In a further 10 patients with gastric CA, these studies were done on TM and TF mucosa 2 cm and > or = 5 cm away from the tumour. In 10 patients and 5 controls, specimens were obtained for electron microscopy as well. Helicobacter pylori infection was diagnosed by means of histology. RESULTS MTT reduction and GST and SOD activities were significantly decreased in TM and TF mucosa in patients with CA compared with controls (P < 0.01). The levels of thiols and catalase activity were significantly increased in CA as compared with controls (P < 0.01). H. pylori positivity did not influence most of these variables but did give significant decrease in MTT reduction in CA (TF) mucosa (P=0.01) and significant increase in thiol levels in CA (TM) mucosa (P=0.04). Electron microscopy showed mitochondrial alterations in tumour cells in all patients and in adjacent mucosa of 10%-50% of the cells. CONCLUSIONS 1) In gastric CA the cancer mucosal cells and the non-involved cells adjacent to the tumour have defective mitochondrial function, which may be due to altered antioxidant defences and possibly altered free radical formation. 2) Ultrastructural mitochondrial abnormalities are shown to parallel these biochemical abnormalities.
Temporary suppression of visual processing in an RSVP task: an attentional blink? .

Through rapid serial visual presentation (RSVP), we asked Ss to identify a partially specified letter (target) and then to detect the presence or absence of a fully specified letter (probe). Whereas targets are accurately identified, probes are poorly detected when they are presented during a 270-ms interval beginning 180 ms after the target. Probes presented immediately after the target or later in the RSVP stream are accurately detected. This temporary reduction in probe detection was not found in conditions in which a brief blank interval followed the target or Ss were not required to identify the target. The data suggest that the presentation of stimuli after the target but before target-identification processes are complete produces interference at a letter-recognition stage. This interference may cause the temporary suppression of visual attention mechanisms observed in the present study.
The relationship between staff stress and team climate in residential services

This study explored the relationship between stress and team climate in care staff working with learning disabled people. A questionnaire was used to collect information in four community residences. The results suggested that there were no significant differences between mean levels of stress, anxiety and depression in different groups of staff. However, variability was seen within the participants across the four facilities. An examination of the relationship between stress and team climate suggested that the higher the stress the worse the perceived team climate. A correlational analysis indicated that the way individuals view aspects of the organisational climate of these facilities had some impact upon their well-being. The implications of these findings are discussed.
Reproduction and transformation of inequalities in schooling: the transformative potential of the theoretical constructs of Bourdieu

This article is concerned with the theoretical constructs of Bourdieu and their contribution to understanding the reproduction of social and cultural inequalities in schooling. While Bourdieu has been criticised for his reproductive emphasis, this article proposes that there is transformative potential in his theoretical constructs and that these suggest possibilities for schools and teachers to improve the educational outcomes of marginalised students. The article draws together three areas of contribution to this theme of transformation; beginning by characterising habitus as constituted by reproductive and transformative traits and considering the possibilities for the restructuring of students’ habitus. This is followed by a discussion of cultural capital and the way that teachers can draw upon a variety of cultural capitals to act as agents of transformation rather than reproduction. The article concludes by considering the necessity of a transformation of the field to improve the educational outcomes of marginalised students.
THE UPTAKE OF PURINES BY RAT BRAIN IN VIVO AND IN VITRO

Abstract— The uptake of [14C]guanine and some of its [14C]‐labelled derivatives into rat brain was studied in vivo and in vitro. In vivo guanine, guanosine, and hypoxanthine penetrated the brain of adult rats to a very small extent. Inosine was taken up somewhat better. In young animals, also, guanosine was taken up poorly, but guanine was taken up fairly well. When guanine was administered to adult animals, only guanine was found in the brain. In young animals, by contrast, radioactivity from guanine appeared in guanosine and in guanine nucleotides, but no free guanine was found. In vitro guanine was taken up much better and, in fact, remained mostly as guanine in slices from 10‐day‐old rats. The in vitro conversion of guanine to GMP and its incorporation into RNA was unimpaired by the addition of unlabelled guanosine, an indication that guanine was converted directly to GMP. The uptake of guanine in vitro was not subject to competitive inhibition or influenced by the presence of dinitrophenol. This finding suggested that guanine entered the slice by simple diffusion.
A Narrow Escape

Although limited research exists on the appropriateness of Advanced Placement (AP) and International Baccalaureate (IB) programs for gifted secondary learners, these courses serve as the primary methods of meeting the needs of gifted students in most high schools. This qualitative study investigates how a broad range of gifted secondary students perceives and evaluates the curriculum, instruction, and environments within AP and IB courses. Interviews with 200 students in 23 U.S. high schools revealed that although students believe that AP and IB courses provide a greater level of academic challenge and more favorable learning environments than other existing high school courses, the curriculum and instruction within AP and IB courses are not a good fit for all learners, particularly those from traditionally underserved populations. Recommendations for increasing the fit of AP and IB courses for—and consequently for increasing the participation of—students from traditionally underserved populations are discussed. Putting the Research to Use: Nearly all of the students in our study indicated that AP and IB courses were the first courses in which they experienced genuine challenge, and the first academic environments in which they felt comfortable with their advanced abilities and academic interests. This indicates that many gifted students have to wait until the last few years of their school careers to encounter courses appropriately matched to their needs. Infusing greater rigor into the K-12 curriculum and allowing gifted students to spend at least part of the day with like-ability peers would go a long way in ensuring that gifted students' academic and social/emotional needs are being met throughout their school careers. Additionally, many former AP and IB students indicated that these lecture-heavy courses were not a good fit for their preferred modes of learning, suggesting a need for a broader range of gifted services at the high school level than AP and IB courses alone.
Weight-bearing shifts of hemiparetic and healthy adults upon stepping on stairs of various heights

Objective: To examine and compare the effect of stepping on stairs of various heights on lower extremity weight bearing in hemiparetic patients. Setting: Flieman Geriatric Rehabilitation Hospital, Haifa, Israel. Subjects: Fifteen ambulatory hemiparetic patients following an acute cerebrovascular accident, and 16 age-matched healthy controls. Interventions: Each subject was tested twice on two consecutive days in five weight-bearing positions which included level stance and stepping with either leg on 10-cm- and 17-cm-high steps. Data concerning weight distribution on the lower extremities were collected by two computerized forceplates. Main outcome measure: Weight borne by each foot expressed as percentage of overall body weight. Results: In the attempted symmetrical level stance, the percentage of body weight borne by the paretic limb of the stroke patients was significantly lower than that of the nonparetic limb. Placing one foot on a step induced a weight shift to the foot placed on the floor regardless of step height. Weight shifting to the paretic limb was, however, significantly lower than to the nonparetic limb. Weight shifting to the nonparetic limb was significantly lower than to the corresponding limb of healthy individuals. Step height had no significant effect on weight distributions on the feet. Conclusions: Raising a foot on a step appears to be an appropriate strategy for weight shift training of stroke patients. Since weight shifting to both the paretic and nonparetic limb of stroke patients is impaired, treatment strategies should include training in weight shifting to both lower extremities.
The Circadian System of Ruin Lizards: A Seasonally Changing Neuroendocrine Loop?

Previous studies have shown that the amplitude of daily melatonin production in cultured ruin lizard pineal organs explanted in the summer is significantly higher than that from organs explanted in the winter. To test whether seasonal photoperiodic changes are decoded autonomously by the pineal gland, pineals explanted in summer were cultured in vitro and exposed to changes between winter and summer photoperiods. The changes in photoperiod duration did not affect the daily profiles of in vitro melatonin production. The discrepancy between the present in vitro results and those from lizards exposed to winter or summer photoperiods before pineal explantation supports the view that circadian information entering the pineal gland via its innervation is involved in determining seasonal changes of melatonin production in ruin lizards. We further examined whether a central component of the circadian system of ruin lizards, specifically the retinae of the lateral eyes, expresses similar seasonal changes in function as does the pineal gland. We did not find any difference between summer and autumn‐winter in the effectiveness of either bilateral retinalectomy or optic nerve lesion—at the level of the optic chiasm—in altering circadian locomotor behavior in constant conditions. Both surgical procedures mostly induced a shortening of the free‐running period of the locomotor rhythm of similar magnitude in all seasons. Thus, the retinae do not appear to participate in the seasonal reorganization of the circadian system in ruin lizards.
Schistosoma haematobium Treatment in 1–5 Year Old Children: Safety and Efficacy of the Antihelminthic Drug Praziquantel

Background Morbidity due to schistosomiasis is currently controlled by treatment of schistosome infected people with the antihelminthic drug praziquantel (PZQ). Children aged up to 5 years are currently excluded from schistosome control programmes largely due to the lack of PZQ safety data in this age group. This study investigated the safety and efficacy of PZQ treatment in such children. Methods Zimbabwean children aged 1–5 years (n = 104) were treated with PZQ tablets and side effects were assessed by questionnaire administered to their caregivers within 24 hours of taking PZQ. Treatment efficacy was determined 6 weeks after PZQ administration through schistosome egg counts in urine. The change in infection levels in the children 1–5 years old (n = 100) was compared to that in 6–10 year old children (n = 435). Principal Findings Pre-treatment S. haematobium infection intensity in 1–5 year olds was 14.6 eggs/10 ml urine and prevalence was 21%. Of the 104 children, 3.8% reported side effects within 24 hours of taking PZQ treatment. These were stomach ache, loss of appetite, lethargy and inflammation of the face and body. PZQ treatment significantly reduced schistosome infection levels in 1–5 year olds with an egg reduction rate (ERR) of 99% and cure rate (CR) of 92%. This was comparable to the efficacy of praziquantel in 6–10 year olds where ERR was 96% and CR was 67%. Interpretation/Significance PZQ treatment is as safe and efficacious in children aged 1–5 years as it is in older children aged 6–10 years in whom PZQ is the drug of choice for control of schistosome infections.
Transethnic genetic correlation estimates from summary statistics

The increasing number of genetic association studies conducted in multiple populations provides unprecedented opportunity to study how the genetic architecture of complex phenotypes varies between populations, a problem important for both medical and population genetics. Here we develop a method for estimating the transethnic genetic correlation: the correlation of causal variant effect sizes at SNPs common in populations. We take advantage of the entire spectrum of SNP associations and use only summary-level GWAS data. This avoids the computational costs and privacy concerns associated with genotype-level information while remaining scalable to hundreds of thousands of individuals and millions of SNPs. We apply our method to gene expression, rheumatoid arthritis, and type-two diabetes data and overwhelmingly find that the genetic correlation is significantly less than 1. Our method is implemented in a python package called popcorn.
Locking-free adaptive discontinuous Galerkin FEM for linear elasticity problems

An adaptive discontinuous Galerkin finite element method for linear elasticity problems is presented. We develop an a posteriori error estimate and prove its robustness with respect to nearly incompressible materials (absence of volume locking). Furthermore, we present some numerical experiments which illustrate the performance of the scheme on adaptively refined meshes.
Gonotrophic Cycle Estimate for Culex quinquefasciatus in Mérida, Yucatán, México

ABSTRACT West Nile virus (WNV) has been present in the Yucatán State, México, since 2002. Culex quinquefasciatus, one of the main vectors of WNV transmission in the United States, is also common in Mexico and may be a key vector of WNV transmission to humans in the Yucatán. The aim of this study was to determine the length of the gonotrophic cycle and the survival rates of Cx. quinquefasciatus from Mérida, Yucatán, during the rainy versus the dry season. Mosquitoes were collected during 25-day periods in October (rainy season) and in April (dry season), and captured females were classified by abdominal appearance (freshly fed, late-stage fed, half gravid, and subgravid). To determine the age structure as nulliparous and parous females and to calculate the gonotrophic cycle through a time series and the mosquito survival, we used Davidson formulae. Also, vitellogenesis analysis to monitor egg maturity was conducted during both seasons. Cross-correlation data suggested a similar length of the gonotrophic cycle (4 days) in both seasons. Oogenic development required a minimum of 72 h in each season. However, survival of the mosquito population collected in the rainy season was significantly higher (0.91) with a mean temperature of 28 ± 1.57°C than was survival in the dry season (0.78) with a mean temperature of 29 ± 1.10°C. Survival, although higher during the rainy season, did not influence the length of the gonotrophic cycle of Cx. quinquefasciatus in Yucatán.
Confucius Institutes: Distributed leadership and knowledge sharing in a worldwide network

– The purpose of this paper is to focus on Confucius Institutes and assess the applicability of theories of leadership and knowledge sharing to multinational organizations and worldwide networks. Growth of multinational trade and decrease in international tension have facilitated the globalization of both profit‐seeking and non‐profit organizations. Changes in economic and political environment have also blurred the divide in management practices between these organizations. , – The research applies recent theoretical developments to analyze leadership and knowledge sharing of the highly successful Confucius Institutes. Operational similarities and differences between this global learning organization and multinational businesses are evaluated. , – Many similarities exist between the operations of the Confucius Institutes and multinational businesses. For both, strategic goals are achieved through the promotion of global expansion and the management practices of distributed leadership and knowledge sharing. The study makes clear the successful application of distributed leadership to a worldwide network. The Confucius Institutes reflect the cultural and social changes in China, combined with influences of global cultures. Findings suggest that distributed leadership is a suitable management style for coping with variant cultural and socio‐political conditions globally. This leadership style, combined with a knowledge‐sharing network, is also suitable for the situational variables encountered in making thousands of decisions across hundreds of global locations by both learning institutions and business organizations. , – The paper explores a relatively new area of the similarities and differences between global non‐profit and business networks as learning organizations. The study is of value to both those managing and those studying such organizations.
Personality Correlates of Alcohol Consumption and Aggression in a Hispanic College Population

The authors examined the association between alcohol consumption and aggression from a personality trait perspective with 92 self-identified Hispanic college students. They partially replicated a study by Quigley, Corbett, and Tedeshi, which examined the relationships between desired image of power, alcohol expectancies, and alcohol-related aggression. Among male participants, impulsivity did not correlate with either alcohol consumption or frequency of fighting, whereas with females there was a strong positive correlation between impulsivity and alcohol consumption and a weak positive correlation between impulsivity and frequency of fighting. When the results were compared to those of Quigley et al., it was found that the Hispanic participants drank and fought less. The best predictor variable for male alcohol consumption was the desire to be viewed as “tough. ” “Activity” was the primary predictor for female alcohol consumption.
Recovery of Mycobacterium bovis from Soft Fresh Cheese Originating in Mexico

ABSTRACT Recent outbreaks of human tuberculosis in the United States caused by Mycobacterium bovis have implicated cheese originating in Mexico as a source of these infections. A total of 203 samples of cheese originating in Mexico were cultured, and M. bovis was recovered from one specimen. Therefore, M. bovis can be recovered from cheese and may be a source of human infections.
Research Report: the Innovating School Head: Autocratic Initiator or Catalyst of Co-Operation?

and staff when interviewed during a research project on the ’Role of the Secondary School Head’. Quantitative findings of the study are then presented and discussed. Links are established between the head’s innovating stance within the school and (1) aspects of his external professional role, (2) his informal interaction with colleagues in the staff common room. A similar relationship with formal staff participation is not established, but significant differences between the responses of heads and staff are noted and discussed. The results obtained are claimed to be consistent with the view
Implicit Attitudes Toward the Self Over Time

Three studies examined whether people hold meaningful implicit attitudes toward future selves. Two approaches to measuring implicit future self-esteem were developed (individualized and generic Implicit Association Tests) and attitudes toward the self in ten (Studies 1 and 2) or one years' time (Study 3) were assessed. We predicted that implicit future self-esteem would be less strongly influenced by self-enhancement and wishful thinking than explicit future self-esteem. Consistent with this expectation, people's explicit self-esteem was more positive toward their future than their current self, whereas implicit future self-esteem was not enhanced relative to the present. As expected, implicit (but not explicit) future self-esteem predicted academic and health motivation, as well as greater elaboration of, and more structure in, future goals. In contrast, explicit (but not implicit) future self-esteem predicted future-oriented measures more linked to wishful thinking: positive fantasies and general optimism. These results suggest that implicit future self-esteem is meaningfully distinct from explicit future self-esteem and has implications for motivation and goals.
Traffic Demand and Energy Efficiency in Heterogeneous Cellular Mobile Radio Networks

Optimization of the energy efficiency is considered not only to positively contribute to the ecological assessment, but gains in importance from operator's point of view as well, since energy costs for running a mobile radio network have an increasing share of the operational expenditure. From this perspective, the utilization of small, low power base stations is regarded as a promising strategy to enhance a network's throughput and to increase the energy efficiency. In this paper we investigate on the efficiency of homogeneous and heterogeneous networks consisting of a varying number of micro sites with regard to traffic load conditions.