Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibiting MAP Kinase Activity Prevents Calcium Transients and Mitosis Entry in Early Sea Urchin Embryos*

A transient calcium increase triggers nuclear envelope breakdown (mitosis entry) in sea urchin embryos. Cdk1/cyclin B kinase activation is also known to be required for mitosis entry. More recently, MAP kinase activity has also been shown to increase during mitosis. In sea urchin embryos, both kinases show a similar activation profile, peaking at the time of mitosis entry. We tested whether the activity of both kinases is required for mitosis entry and whether either kinase controls mitotic calcium signals. We found that reducing the activity of either mitotic kinase prevents nuclear envelope breakdown, despite the presence of a calcium transient, when cdk1/cyclin B kinase activity is alone inhibited. When MAP kinase activity alone was inhibited, the calcium signal was absent, suggesting that MAP kinase activity is required to generate the calcium transient that triggers nuclear envelope breakdown. However, increasing intracellular free calcium by microinjection of calcium buffers or InsP3 while MAP kinase was inhibited did not itself induce nuclear envelope breakdown, indicating that additional MAP kinase-regulated events are necessary. After MAP kinase inhibition early in the cell cycle, the early events of the cell cycle (pronuclear migration/fusion and DNA synthesis) were unaffected, but chromosome condensation and spindle assembly are prevented. These data indicate that in sea urchin embryos, MAP kinase activity is part of a signaling complex alongside two components previously shown to be essential for entry into mitosis: the calcium transient and the increase in cdk1/cyclinB kinase activity.
SUMO and SUMO-Conjugating Enzyme E2 UBC9 Are Involved in White Spot Syndrome Virus Infection in Fenneropenaeus chinensis

In previous work, small ubiquitin-like modifier (SUMO) in hemocytes of Chinese shrimp Fenneropenaeus chinensis was found to be up-regulated post-white spot syndrome virus (WSSV) infection using proteomic approach. However, the role of SUMO in viral infection is still unclear. In the present work, full length cDNAs of SUMO (FcSUMO) and SUMO-conjugating enzyme E2 UBC9 (FcUBC9) were cloned from F. chinensis using rapid amplification of cDNA ends approach. The open reading frame (ORF) of FcSUMO encoded a 93 amino acids peptide with the predicted molecular weight (M.W) of 10.55 kDa, and the UBC9 ORF encoded a 160 amino acids peptide with the predicted M.W of 18.35 kDa. By quantitative real-time RT-PCR, higher mRNA transcription levels of FcSUMO and FcUBC9 were detected in hemocytes and ovary of F. chinensis, and the two genes were significantly up-regulated post WSSV infection. Subsequently, the recombinant proteins of FcSUMO and FcUBC9 were expressed in Escherichia coli BL21 (DE3), and employed as immunogens for the production of polyclonal antibody (PAb). Indirect immunofluorescence assay revealed that the FcSUMO and UBC9 proteins were mainly located in the hemocytes nuclei. By western blotting, a 13.5 kDa protein and a 18.7 kDa protein in hemocytes were recognized by the PAb against SUMO or UBC9 respectively. Furthermore, gene silencing of FcSUMO and FcUBC9 were performed using RNA interference, and the results showed that the number of WSSV copies and the viral gene expressions were inhibited by knockdown of either SUMO or UBC9, and the mortalities of shrimp were also reduced. These results indicated that FcSUMO and FcUBC9 played important roles in WSSV infection.
What can graph theory tell us about word learning and lexical retrieval?

PURPOSE Graph theory and the new science of networks provide a mathematically rigorous approach to examine the development and organization of complex systems. These tools were applied to the mental lexicon to examine the organization of words in the lexicon and to explore how that structure might influence the acquisition and retrieval of phonological word-forms. METHOD Pajek, a program for large network analysis and visualization (V. Batagelj & A. Mvrar, 1998), was used to examine several characteristics of a network derived from a computerized database of the adult lexicon. Nodes in the network represented words, and a link connected two nodes if the words were phonological neighbors. RESULTS The average path length and clustering coefficient suggest that the phonological network exhibits small-world characteristics. The degree distribution was fit better by an exponential rather than a power-law function. Finally, the network exhibited assortative mixing by degree. Some of these structural characteristics were also found in graphs that were formed by 2 simple stochastic processes suggesting that similar processes might influence the development of the lexicon. CONCLUSIONS The graph theoretic perspective may provide novel insights about the mental lexicon and lead to future studies that help us better understand language development and processing.
Effects of metals on Legionella pneumophila growth in drinking water plumbing systems

An investigation of the chemical environment and growth of Legionella pneumophila in plumbing systems was conducted to gain a better understanding of its ecology in this habitat. Water samples were collected from hospital and institutional hot-water tanks known to have supported L. pneumophila and were analyzed for 23 chemical parameters. The chemical environment of these tanks was found to vary extensively, with the concentrations of certain metals reaching relatively high levels due to corrosion. The effect of various chemical conditions on L. pneumophila growth was then examined by observing its multiplication in the chemically analyzed hot-water tank samples after sterilization and reinoculation with L. pneumophila. L. pneumophila and associated microbiota used in these experiments were obtained from a hot-water tank. These stains were maintained in tap water and had never been passaged on agar. The results of the growth studies indicate that although elevated concentrations of a number of metals are toxic, lower levels of certain metals such as iron, zinc, and potassium enhance growth of naturally occurring L. pneumophila. Parallel observations on accompanying non-Legionellaceae bacteria failed to show the same relationship. These findings suggest that metal plumbing components and associated corrosion products are important factors in the survival and growth of L. pneumophila in plumbing systems and may also be important in related habitats such as cooling towers and air-conditioning systems.
Redox systematics of a magma ocean with variable pressure-temperature gradients and composition

Oxygen fugacity in metal-bearing systems controls some fundamental aspects of the geochemistry of the early Earth, such as the FeO and siderophile trace element content of the mantle, volatile species that influence atmospheric composition, and conditions for organic compounds synthesis. Redox and metal-silicate equilibria in the early Earth are sensitive to oxygen fugacity (fO2), yet are poorly constrained in modeling and experimentation. High pressure and temperature experimentation and modeling in metal-silicate systems usually employs an approximation approach for estimating fO2 that is based on the ratio of Fe and FeO [called “ΔIW (ratio)” hereafter]. We present a new approach that utilizes free energy and activity modeling of the equilibrium: Fe + SiO2 + O2 = Fe2SiO4 to calculate absolute fO2 and relative to the iron-wüstite (IW) buffer at pressure and temperature [ΔIW (P,T)]. This equilibrium is considered across a wide range of pressures and temperatures, including up to the liquidus temperature of peridotite (4,000 K at 50 GPa). Application of ΔIW (ratio) to metal-silicate experiments can be three or four orders of magnitude different from ΔIW (P,T) values calculated using free energy and activity modeling. We will also use this approach to consider the variation in oxygen fugacity in a magma ocean scenario for various thermal structures for the early Earth: hot liquidus gradient, 100 °C below the liquidus, hot and cool adiabatic gradients, and a cool subsolidus adiabat. The results are used to assess the effect of increasing P and T, changing silicate composition during accretion, and related to current models for accretion and core formation in the Earth. The fO2 in a deep magma ocean scenario may become lower relative to the IW buffer at hotter and deeper conditions, which could include metal entrainment scenarios. Therefore, fO2 may evolve from high to low fO2 during Earth (and other differentiated bodies) accretion. Any modeling of core formation and metal-silicate equilibrium should take these effects into account.
Joint prediction in MST-style discourse parsing for argumentation mining

We introduce a new approach to argumentation mining that we applied to a parallel German/English corpus of short texts annotated with argumentation structure. We focus on structure prediction, which we break into a number of subtasks: relation identification, central claim identification, role classification, and function classification. Our new model jointly predicts different aspects of the structure by combining the different subtask predictions in the edge weights of an evidence graph; we then apply a standard MST decoding algorithm. This model not only outperforms two reasonable baselines and two datadriven models of global argument structure for the difficult subtask of relation identification, but also improves the results for central claim identification and function classification and it compares favorably to a complex mstparser pipeline.
Peripheralization: Theoretical Concepts Explaining Socio-Spatial Inequalities

The current increase in socio-spatial inequalities in Europe has led to a revival of the terms "peripheralization" and "marginalization" in spatial research. In contrast to the geographical notion of a "periphery", which is synonymous with distance to a centre and being situated on the fringes of a city, region or nation, research on "peripheralization" describes the production of peripheries through social relations and their spatial implications. The main part of the article provides a critical review of theoretical concepts which attempt to explain socio-spatial disparities between centralization and peripheralization processes. This includes theories of economic polarization, social inequality and political power. Building on this, a multidimensional concept of socio-spatial polarization is outlined, one which comprises processes of centralization and peripheralization in economic, social and political dimensions. Finally, implications are drawn for spatial planning regarding the polarization between metropolitan and non-metropolitan regions and areas for further research are highlighted .
Do social and cultural factors perpetuate gender based violence in Malawi

Gender based violence in Malawi exist at a level that requires special acknowledgement. A survey was conducted to assess how social and cultural factors affect gender-based violence in Malawi. The study revealed that both men and women are victims of gender based violence although women bare the brunt of the practice. Men abuse women through battery, use of abusive language, not providing some requirements and overworking them. Women abuse men by not giving them food and engaging in extra marital affairs. The study concluded that there are cultural practices and beliefs that perpetuate gender-based violence and these include “chiongo”-dowry, polygamy, “the notion of household head”, male mobility, forced marriage and not having sex with a woman when she is menstruating and during post-partum abstinence which can force a man to have extra-marital sex. Keywords : socio-cultural factors, women, gender based violence, Malawi Gender & Behaviour Vol. 6 (2) 2008: pp. 1884-1896
Spitzenkörper Localization and Intracellular Traffic of Green Fluorescent Protein-Labeled CHS-3 and CHS-6 Chitin Synthases in Living Hyphae of Neurospora crassa

ABSTRACT The subcellular location and traffic of two selected chitin synthases (CHS) from Neurospora crassa, CHS-3 and CHS-6, labeled with green fluorescent protein (GFP), were studied by high-resolution confocal laser scanning microscopy. While we found some differences in the overall distribution patterns and appearances of CHS-3-GFP and CHS-6-GFP, most features were similar and were observed consistently. At the hyphal apex, fluorescence congregated into a conspicuous single body corresponding to the location of the Spitzenkörper (Spk). In distal regions (beyond 40 μm from the apex), CHS-GFP revealed a network of large endomembranous compartments that was predominantly comprised of irregular tubular shapes, while some compartments were distinctly spherical. In the distal subapex (20 to 40 μm from the apex), fluorescence was observed in globular bodies that appeared to disintegrate into vesicles as they advanced forward until reaching the proximal subapex (5 to 20 μm from the apex). CHS-GFP was also conspicuously found delineating developing septa. Analysis of fluorescence recovery after photobleaching suggested that the fluorescence of the Spk originated from the advancing population of microvesicles (chitosomes) in the subapex. The inability of brefeldin A to interfere with the traffic of CHS-containing microvesicles and the lack of colocalization of CHS-GFP with the endoplasmic reticulum (ER)-Golgi body fluorescent dyes lend support to the idea that CHS proteins are delivered to the cell surface via an alternative route distinct from the classical ER-Golgi body secretory pathway.
Self-Securing Network Interfaces: What, Why and How (CMU-CS-02-144)

Self-securing network interfaces (NIs) examine the packets that they move between network links and host software, looking for and potentially blocking malicious network activity. This paper describes self-securing network interfaces, their features, and examples of how these features allow administrators to more effectively spot and contain malicious network activity. We present a software architecture for self-securing NIs that separates scanning software into applications (called scanners) running on an NI kernel. The resulting scanner API simplifies the construction of scanning software and allows its powers to be contained even if it is subverted. We illustrate the potential via a prototype selfsecuring NI and two example scanners: one that identifies and blocks known e-mail viruses and one that identifies and inhibits rapidly-propagating worms like Code-Red. We thank the members and companies of the PDL Consortium (including EMC, Hewlett-Packard, Hitachi, IBM, Intel, Network Appliance, Panasas, Seagate, Sun, and Veritas) for their interest, insights, feedback, and support. We thank IBM and Intel for hardware grants supporting our research efforts. This material is based on research sponsored by the Air Force Research Laboratory, under agreement number F49620-01-1-0433. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of the Air Force Research Laboratory or the U.S. Government.
The Power of Living the Writerly Life: A Group Model for Women Writers

Flourishing as a tenure-track faculty member in academia is no small feat. Best practices in writing are often neglected as faculty struggle to balance competing demands on their time. For women who try to balance both home and work responsibilities, this task can be overwhelming. This study documents the outcomes of a writers’ group formed to increase the scholarly output of its members. Findings suggest that this writers’ group model not only has potential to increase writing productivity but also offers the possibility of producing unanticipated outcomes including support and encouragement leading to greater professional and personal balance.
Aspirate from human stented saphenous vein grafts induces epicardial coronary vasoconstriction and impairs perfusion and left ventricular function in rat bioassay hearts with pharmacologically induced endothelial dysfunction

Stent implantation into aortocoronary saphenous vein grafts (SVG) releases particulate debris and soluble vasoactive mediators, for example, serotonin. We now analyzed effects of the soluble mediators released into the coronary arterial blood during stent implantation on vasomotion of isolated rat epicardial coronary artery segments and on coronary flow and left ventricular developed pressure in isolated perfused rat hearts. Coronary blood was retrieved during percutaneous SVG intervention using a distal occlusion/aspiration protection device in nine symptomatic patients with stable angina pectoris and a flow‐limiting SVG stenosis. The blood was separated into particulate debris and plasma. Responses to coronary plasma were determined in isolated rat epicardial coronary arteries and in isolated, constant pressure‐perfused rat hearts (±nitric oxide synthase [NOS] inhibition and ±serotonin receptor blockade, respectively). Coronary aspirate plasma taken after stent implantation induced a stronger vasoconstriction of rat epicardial coronary arteries (52 ± 8% of maximal potassium chloride induced vasoconstriction [% KClmax = 100%]) than plasma taken before stent implantation (12 ± 8% of KClmax); NOS inhibition augmented this vasoconstrictor response (to 110 ± 15% and 24 ± 9% of KClmax). Coronary aspirate plasma taken after stent implantation reduced in isolated perfused rat hearts only under NOS inhibition coronary flow by 17 ± 3% and left ventricular developed pressure by 25 ± 4%. Blockade of serotonin receptors abrogated these effects. Coronary aspirate plasma taken after stent implantation induces vasoconstriction in isolated rat epicardial coronary arteries and reduces coronary flow and left ventricular developed pressure in isolated perfused rat hearts with pharmacologically induced endothelial dysfunction.
Social Networks, Social Support, and Self-Ratings of Health among the Elderly

Social networks and the support they may provide have been shown to be important determinants of the health of the elderly. This study compares structural and support aspects of those networks in their relationship with self-latings of health among 200 elderly residents of the city of Jerusalem. Perceived social support was found to be a more important predictor of health than were measures of network structure. Further, the relation between support and health was a function of the relation between support and both psychological state and functional status.
Indoor Positioning Using Visible LED Lights

Visible light LEDs, due to their numerous advantages, are expected to become the dominant indoor lighting technology. These lights can also be switched ON/OFF at high frequency, enabling their additional use for wireless communication and indoor positioning. In this article, visible LED light--based indoor positioning systems are surveyed and classified into two broad categories based on the receiver structure. The basic principle and architecture of each design category, along with various position computation algorithms, are discussed and compared. Finally, several new research, implementation, commercialization, and standardization challenges are identified and highlighted for this relatively novel and interesting indoor localization technology.
A Comparison of Global Ratings and Checklist Scores from an Undergraduate Assessment Using an Anesthesia Simulator

Purpose To determine the correlation between global ratings and criterion-based checklist scores, and inter-rater reliability of global ratings and criterion-based checklist scores, in a performance assessment using an anesthesia simulator. Method All final-year medical students at the University of Toronto were invited to work through a 15-minute faculty-facilitated scenario using an anesthesia simulator. Students' performances were videotaped and analyzed by two faculty using a 25-point criterion-based checklist and a five-point global rating of competency (1 = clear failure, 5 = superior performance). Correlations between global ratings and checklist scores, as well as specific performance competencies (knowledge, technical skills, and judgment), were determined. Checklist and global scores were converted to percentages; means of the two marks were compared. Mean reliability of a single rater for both checklist and global ratings was determined. Results The correlation between checklist and global ratings was . 74. Mean ratings of both checklist and global scores were low (58.67, SD = 14.96, and 57.08, SD = 24.27, respectively); these differences were not statistically significant. For a single rater, the mean reliability score across rater pairs for checklist scores was . 77 (range .58–.93). Mean reliability score across rater pairs for global ratings was . 62 (.40–.77). Global ratings correlated more highly with technical skills and judgment (r = .51 and r = .53, respectively) than with knowledge. (r = .24) Conclusion Inter-rater reliability was higher for checklist scores than for global ratings; however, global ratings demonstrated acceptable inter-rater reliability and may be useful for competency assessment in performance assessments using simulators.
Effect of health education on knowledge, attitude and practices of personal hygiene among secondary school students in rural Sokoto, North West, Nigeria.

BACKGROUND Personal hygiene related diseases are serious public health problems in developing countries. OBJECTIVE To assess the effect of health education on knowledge, attitude, and practices of personal hygiene among rural secondary school students in Sokoto state, north western Nigeria. METHODS A quasi-experimental controlled study with pretest and post-test design was carried out. A total 120 subjects per group were selected by multistage sampling technique. Two pre-tested instruments, structured interviewer-administered questionnaire and observer's checklist for personal hygiene practices were used for data collection. Health education intervention was carried out one week after baseline data collection and repeated after four weeks for the intervention group only. Postintervention data collection was carried out in both intervention and control groups three months after the 2nd intervention. However, for ethical consideration, the control group was also provided with the health education intervention similar to that provided to the intervention group. RESULTS A total of 120 and 116 subjects in the intervention and control groups respectively participated in all phases of the study. The mean knowledge score (%) of the study subjects was high and similar (73.18 +/- 25.82; 77.06 +/- 21.79; p = 0.21) in both the intervention and control groups at baseline, but differed very significantly (91.16 +/- 11.03; 81.74 +/- 21.78; p < 0.0001) at post-intervention. The mean attitude score (%) was high but differ significantly (82.6 +/- 12.73; 86.38 +/- 10.74; p = 0.01) between the intervention and control groups at baseline, and further differed very significantly (95.5 +/- 3.66; 86.90 +/- 10.44; p < 0.0001) at post intervention. The mean reported practice score (%) was high but differ significantly (94.86 +/- 3.00; 93.25 +/- 6.31; p = 0.01) between the intervention and control groups at baseline, and further differed very significantly (96.68 +/- 4.08; 93.7 +/- 6.28; p < 0.0001) at post-intervention. The mean observed practice score (%) was high and similar (57.50 +/- 29.65; 61.35 +/- 28.49; p = 0.31) in both the intervention and control groups at baseline, but differed very significantly (88.20 +/- 9.99; 62.10 +/- 26.94; p < 0.0001) at post-intervention. CONCLUSION Health education intervention had impact on knowledge base, attitude and practice of study subjects in intervention group compared to control group. Personal hygiene health education is therefore recommended to be taught in secondary schools.