Miracle-dz/UniFAR-FTUs huggingface dataset:
Creatine metabolism in skeletal muscle. I. Creatine movement across muscle membranes.

Abstract In studies in vitro, creatine-1-14C entered the extensor digitorum longus of young rats by a saturable process which had an apparent Vmax of 0.6 mmole per liter of intracellular water per hour and an apparent Km of 5 x 10-4 M. Creatine entry by nonsaturable processes was negligible at physiologic external creatine concentrations. Anaerobiosis, 1 x 10-3 M 2,4-dinitrophenol, and cooling each reduced the intracellular accumulation of creatine-14C. The apparent Q10o for entry was 2.7. Loss of creatine from the muscle accelerated rapidly during incubation at 37° and exceeded the rate of creatine entry, but no connection between entry and loss was apparent. The large creatine loss in vitro was considered to be an artifact because it accelerated so rapidly during incubation and because it is inconsistent with observations from earlier experiments in vivo which indicate that much of the creatine in skeletal muscle is trapped there. A special mechanism for entry, the saturable process, and intracellular trapping of creatine provide a plausible explanation for the high creatine content of skeletal muscle.
Performance analysis of a synchronous, circuit-switched interconnection cached network

In many parallel applications, each computation entity (process, thread etc.) switches the bulk of its communication between a small group of other entities. We call this phenomenon switching locality. The Interconnection Cached Network (ICN) is a reconfigurable network especially suited for exploiting switching locality. It consists of many small, fast crossbars interconnected by a large, slow switching crossbar. The large crossbar is used for topology reconfiguration and the smaller crossbars for circuit switching. For a large class of communication patterns displaying switching locality (this includes meshes, tori, trees, rings, pyramids, etc.), it is possible to choose appropriate ICN configurations and assignments of processes to processors such that all communication paths pass through two or less switching components. Much of the previous work on performance analysis of networks has assumed random, uniformly distributed communication and is inapplicable to many real-life parallel applications that lack this uniformity. We develop a methodology to analyze the performance of synchronous, circuit switched networks under different communication traffic patterns. We employ this methodology to study the performance of the ICN in comparison to more popular reconfigurable networks: the delta and the crossbar. We choose two different communication patterns—a 2-D torus representing a high degree of switching locality and a fully connected graph representing complete absence of such locality. We show that in the presence of locality, the ICN comes very close to matching the crossbar's performance. This, together with the shorter network cycle period of the ICN, makes it more desirable. In the absence of switching locality, the reconfigurability of the ICN allows for a graceful degradation in performance.
Enhancing the utility of complex‐valued functional magnetic resonance imaging detection of neurobiological processes through postacquisition estimation and correction of dynamic B0 errors and motion

Functional magnetic resonance imaging (fMRI) time series analysis is typically performed using only the magnitude portion of the data. The phase information remains unused largely due to its sensitivity to temporal variations in the magnetic field unrelated to the functional response of interest. These phase changes are commonly the result of physiologic processes such as breathing or motion either inside or outside the imaging field of view. As a result, although the functional phase response carries pertinent physiological information concerning the vasculature, one aspect of which is the location of large draining veins, the full hemodynamic phase response is understudied and is poorly understood, especially in comparison with the magnitude response. It is likely that the magnitude and phase contain disjoint information, which could be used in tandem to better characterize functional hemodynamics. In this work, simulated and human fMRI experimental data are used to demonstrate how statistical analysis of complex‐valued fMRI time series can be problematic, and how robust analysis using these powerful and flexible complex‐valued statistics is possible through postprocessing with correction for dynamic magnetic field fluctuations in conjunction with estimated motion parameters. These techniques require no special pulse sequence modifications and can be applied to any complex‐valued echo planar imaging data set. This analysis shows that the phase component appears to contain information complementary to that in the magnitude and that processing and analysis techniques are available to investigate it in a robust and flexible manner. Hum Brain Mapp, 2012. © 2011 Wiley Periodicals, Inc.
Stable and Rapid Recurrent Processing in Realistic Autoassociative Memories

It is shown that in those autoassociative memories that learn by storing multiple patterns of activity on their recurrent collateral connections, there is a fundamental conflict between dynamical stability and storage capacity. It is then found that the network can nevertheless retrieve many different memory patterns, as predicted by nondynamical analyses, if its firing is regulated by inhibition that is sufficiently multiplicative in nature. Simulations of a model network with integrate-and-fire units confirm that this is a realistic solution to the conflict. The simulations also confirm the earlier analytical result that cued-elicited memory retrieval, which follows an exponential time course, occurs in a time linearly related to the time constant for synaptic conductance inactivation and relatively independent of neuronal time constants and firing levels.
Exploiting Spatial Indexes for Semijoin-Based Join Processing in Distributed Spatial Databases

In a distributed spatial database system, a user may issue a query that relates two spatial relations not stored at the same site. Because of the sheer volume and complexity of spatial data, spatial joins between two spatial relations at different sites are expensive in terms of computational and transmission costs. In this paper, we address the problems of processing spatial joins in a distributed environment. We propose a semijoin-like operator, called the spatial semijoin, to prune away objects that do not contribute to the join result. This operator also reduces both the transmission and local processing costs for a later join operation. However, the cost of the elimination process must be taken into account, and we consider approaches to minimize these overheads. We also study and compare two families of distributed join algorithms that are based on the spatial semijoin operator. The first is based on multi-dimensional approximations obtained from an index such as the R-tree, and the second is based on single-dimensional approximations obtained from object mapping. We have conducted experiments on real data sets and report the results in this paper.
Sexually antagonistic coevolution in insects is associated with only limited morphological diversity

Morphological traits involved in male–female sexual interactions, such as male genitalia, often show rapid divergent evolution. This widespread evolutionary pattern could result from sustained sexually antagonistic coevolution, or from other types of selection such as female choice or selection for species isolation. I reviewed the extensive but under‐utilized taxonomic literature on a selected subset of insects, in which male–female conflict has apparently resulted in antagonistic coevolution in males and females. I checked the sexual morphology of groups comprising 500–1000 species in six orders for three evolutionary trends predicted by the sexually antagonistic coevolution hypothesis: males with species‐specific differences and elaborate morphology in structures that grasp or perforate females in sexual contexts; corresponding female structures with apparently coevolved species‐specific morphology; and potentially defensive designs of female morphology. The expectation was that the predictions were especially likely to be fulfilled in these groups. A largely qualitative overview revealed several surprising patterns: sexually antagonistic coevolution is associated with frequent, relatively weak species‐specific differences in males, but male designs are usually relatively simple and conservative (in contrast to the diverse and elaborate designs common in male structures specialized to contact and hold females in other species, and also in weapons such as horns and pincers used in intra‐specific battles); coevolutionary divergence of females is not common; and defensive female divergence is very uncommon. No cases were found of female defensive devices that can be facultatively deployed. Coevolutionary morphological races may have occurred between males and females of some bugs with traumatic insemination, but apparently as a result of female attempts to control fertilization, rather than to reduce the physical damage and infections resulting from insertion of the male's hypodermic genitalia. In sum, the sexually antagonistic coevolution that probably occurs in these groups has generally not resulted in rapid, sustained evolutionary divergence in male and female external sexual morphology. Several limitations of this study, and directions for further analyses are discussed.
Energy-Driven Pattern Formation

Many physical systems can be modelled by nonconvex variational problems regularized by higher-order terms. Examples include martensitic phase transformation, micromagnetics, and the Ginzburg–Landau model of nucleation. We are interested in the singular limit, when the coefficient of the higher-order term tends to zero. Our attention is on the internal structure of walls, and the character of microstructure when it forms. We also study the pathways of thermally-activated transitions, modeled via the minimization of action rather than energy. Our viewpoint is variational, focusing on matching upper and lower bounds. Mathematics Subject Classification (2000). Primary 49-02, 82-02; Secondary 74N15, 82B24, 82D40.
The Role of Physical Exercise and Inactivity in Pain Recurrence and Absenteeism From Work After Active Outpatient Rehabilitation for Recurrent or Chronic Low Back Pain: A Follow-Up Study

Study Design. An observational follow-up. Objectives. To analyze the role of physical exercise and inactivity on the long-term outcome after active outpatient low back rehabilitation. Summary of Background Data. There is considerable evidence documenting the efficacy of exercise in the conservative treatment of chronic low back pain, but the role of exercises after the guided treatment period on the long-term success and maintenance of the results is not known. Methods. One hundred twenty-five patients with low back pain, who had participated in a 12-week active low back rehabilitation program, were asked about subjective pain and disability on the average of 14 months after the treatment. The outcomes were defined as a recurrence of persistent pain and work absenteeism, and a survival or failure analysis was performed between those who had continued exercising and who had been physically inactive. Results. Recurrences of persistent pain during the follow-up period were fewer (P = 0.03) among those who had maintained regular exercise habits after the treatment than among those who had been physically inactive. Similarly, work absenteeism was less (P < 0.01) among physically active than among physically inactive persons. However, patients with good outcome in pain reduction after low back pain rehabilitation were more likely to participate in physical exercise. Conclusions. Exercises are beneficial after guided treatment in the maintenance of the results of active treatment for recurrent chronic low back pain in the long term, but those with less favorable outcome in rehabilitation are less likely to participate in exercises afterward. In active treatment programs, it is recommended that exercises be incorporated after the guided treatment.
The Use of Personal Digital Assistants at the Point of Care in an Undergraduate Nursing Program

This study examined the relationships between the use of personal digital assistants and self-efficacy and the preparation for medication administration among second-year Bachelor of Science in Nursing students in a medical-surgical clinical environment. By using a controlled experimental method, the study attempted to support claims about the educational benefits of personal digital assistants which have generally been reported in more descriptive and anecdotal formats. The sample consisted of 36 students, of which two groups had personal digital assistants and two groups served as a control. The control groups were provided with paper resources equivalent to the software provided by the personal digital assistants. Findings showed a significant increase in self-efficacy in the groups with personal digital assistants.
Functional importance of telomerase pseudoknot revealed by single-molecule analysis

Telomerase ribonucleoprotein (RNP) employs an RNA subunit to template the addition of telomeric repeats onto chromosome ends. Previous studies have suggested that a region of the RNA downstream of the template may be important for telomerase activity and that the region could fold into a pseudoknot. Whether the pseudoknot motif is formed in the active telomerase RNP and what its functional role is have not yet been conclusively established. Using single-molecule FRET, we show that the isolated pseudoknot sequence stably folds into a pseudoknot. However, in the context of the full-length telomerase RNA, interference by other parts of the RNA prevents the formation of the pseudoknot. The protein subunits of the telomerase holoenzyme counteract RNA-induced misfolding and allow a significant fraction of the RNPs to form the pseudoknot structure. Only those RNP complexes containing a properly folded pseudoknot are catalytically active. These results not only demonstrate the functional importance of the pseudoknot but also reveal the critical role played by telomerase proteins in pseudoknot folding.
Disorders of Glucose Regulation in Adults and Birth Weight

OBJECTIVE—The purpose of this study was to examine the association of birth weight with indexes of glycemia in a population-based survey. RESEARCH DESIGN AND METHODS—A total of 10,788 participants in the Australian Diabetes, Obesity and Lifestyle (AusDiab) Study were asked to complete a birth weight questionnaire. Fasting plasma glucose (FPG), postload glucose (PLG), and A1C were modeled against birth weight. World Health Organization criteria were used to define impaired fasting glucose (IFG), impaired glucose tolerance (IGT), and diabetes. RESULTS—Among 7,157 participants who responded to the questionnaire, 4,502 reported their birth weights, with a mean ± SD of 3.4 ± 0.7 kg. FPG, PLG, and A1C were strongly and inversely correlated with birth weight. The odds ratios (95% CI) for high (>90th sex-specific percentile) FPG, PLG, and A1C were 0.83 (0.71–0.96), 0.74 (0.65–0.84), and 0.81 (0.70–0.94), respectively, for a 1-kg increase in birth weight after adjustment for age and sex. In those with low birth weight (LBW), the risks for having IFG, IGT, and diabetes and for all abnormalities combined were increased by 1.75, 2.22, 2.76, and 2.28, respectively, for women and by 1.40, 1.32, 1.98, and 1.49 for men compared with risks for those with normal birth weight. These trends applied across categories of age and BMI. CONCLUSIONS—In an affluent Western country with a good adult health profile, birth weight has an inverse relationship with indexes of glycemia, and individuals with LBW were predisposed to higher rates of glycemic dysregulation in adult life. These associations were independent of BMI and of other factors significantly correlated with glycemic dysregulation.
Clinical expression of bipolar disorder type I as a function of age and polarity at onset: convergent findings in samples from France and the United States.

BACKGROUND The clinical presentation, course, and comorbidities of bipolar disorder type I are highly heterogeneous, and this variability remains poorly predictable. Certain onset characteristics (eg, age and polarity at onset) may delineate subgroups differing in clinical expression and outcome. METHOD We retrospectively investigated the association between both age and polarity at onset and the clinical characteristics of bipolar I disorder (DSM-IV) in 2 independent adult samples: 480 French patients assessed in 1992-2006 (patients had been recruited from 3 university-affiliated psychiatry departments) and 714 US patients assessed in 1991-2003 (data were extracted from the Bipolar Disorder Phenome Database). RESULTS Polarity at onset correlated with subsequent predominance (P < .001). Most patients experienced a depressive onset (57.9% in France vs 71.0% in the United States; P < .001) associated with a higher density of depressive episodes, suicidal behavior, and alcohol misuse. A manic onset was associated with a higher density of manic episodes. Early onset was frequent in both countries (42% in France vs 68% in the United States; P < .001) and was associated with suicidal behavior and cannabis and cocaine/opiate misuse. Sensitivity for the prediction of clinical characteristics was 1%-35% for age at onset and 26%-47% for polarity at onset. CONCLUSIONS Onset characteristics are associated with subsequent predominant polarity, suicidal behavior, and substance misuse in bipolar I disorder. These findings may facilitate personalized treatment strategies based on type of onset and may also facilitate early focused strategies for preventing comorbidity. Given the relatively low sensitivity and specificity of these onset characteristics for predicting clinical variables, the relevance of age and polarity at onset as specifiers in nosographical classifications will require further studies. However, polarity at onset may be the more relevant specifier, with further investigation required for age at onset.
Solid-state NMR studies of the secondary structure of a mutant prion protein fragment of 55 residues that induces neurodegeneration

The secondary structure of a 55-residue fragment of the mouse prion protein, MoPrP(89–143), was studied in randomly aggregated (dried from water) and fibrillar (precipitated from water/acetonitrile) forms by 13C solid-state NMR. Recent studies have shown that the fibrillar form of the P101L mutant of MoPrP(89–143) is capable of inducing prion disease in transgenic mice, whereas unaggregated or randomly aggregated samples do not provoke disease. Through analysis of 13C chemical shifts, we have determined that both wild-type and mutant sequence MoPrP(89–143) form a mixture of β-sheet and α-helical conformations in the randomly aggregated state although the β-sheet content in MoPrP(89–143, P101L) is significantly higher than in the wild-type peptide. In a fibrillar state, MoPrP(89–143, P101L) is completely converted into β-sheet, suggesting that the formation of a specific β-sheet structure may be required for the peptide to induce disease. Studies of an analogous peptide from Syrian hamster PrP verify that sequence alterations in residues 101–117 affect the conformation of aggregated forms of the peptides.
Near-Isogenic Cry1F-Resistant Strain of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Investigate Fitness Cost Associated With Resistance in Brazil

Abstract Field-evolved resistance to Cry1F maize in Spodoptera frugiperda (J.E. Smith) populations in Brazil was reported in 2014. In this study, to investigate fitness costs, we constructed a near-isogenic S. frugiperda- resistant strain (R-Cry1F) using Cry1F-resistant and Cry1F-susceptible strains sharing a close genetic background. A near-isogenic R-Cry1F strain was obtained by eight repeated backcrossings, each followed by sib-mating and selection among resistant and susceptible strains. Fitness cost parameters were evaluated by comparing the biological performance of resistant, susceptible, and heterozygous strains on artificial diet. Fitness parameters monitored included development time and survival rates of egg, larval, pupal, and egg-to-adult periods; sex ratio; adult longevity; timing of preoviposition, oviposition, and postoviposition; fecundity; and fertility. A fertility life table was also calculated. The near-isogenic R-Cry1F strain showed lower survival rate of eggs (32%), when compared with Sus and reciprocal crosses (41 and 55%, respectively). The number of R-Cry1F insects that completed the life cycle was reduced to ∼25%, compared with the Sus strain with ∼32% reaching the adult stage. The mean generation time (T) of R-Cry1F strain was ∼2 d shorter than R-Cry1F♂ × Sus♀ and Sus strains. The reproductive parameters of R-Cry1F strain were similar to the Sus strain. However, fewer females were produced by R-Cry1F strain than R-Cry1F♀ × Sus♂ and more females than R-Cry1F♂ × Sus♀. In summary, no relevant fitness costs are observed in a near-isogenic Cry1F-resistant strain of S. frugiperda, indicating stability of resistance to Cry1F protein in Brazilian populations of this species in the absence of selection pressure.
Inference for Parameters Defined by Moment Inequalities: A Recommended Moment Selection Procedure

This paper is concerned with tests and confidence intervals for parameters that are not necessarily identified and are defined by moment inequalities. In the literature, different test statistics, critical value methods, and implementation methods (i.e., the asymptotic distribution versus the bootstrap) have been proposed. In this paper, we compare these methods. We provide a recommended test statistic, moment selection critical value method, and implementation method. We provide data-dependent procedures for choosing the key moment selection tuning parameter kappa and a size-correction factor eta.
Intrathymic clonal deletion of V beta 6+ T cells in cyclophosphamide- induced tolerance to H-2-compatible, Mls-disparate antigens

When C3H (H-2k, Mls-1b) mice were primed intravenously with 10(8) viable spleen cells from AKR (H-2k, Mls-1a) and treated intraperitoneally with 200 mg/kg of cyclophosphamide (CP) 2 d later, not only a long-lasting skin allograft tolerance but also a tolerance in mixed lymphocyte reaction to Mls-1a-encoded antigens was established. The cellular mechanisms of CP-induced tolerance were examined by assessing the V beta 6-bearing T cells that are strongly correlated with reactivity to Mls-1a-encoded antigens bound to MHC class II molecules. At the relatively early stage (2 or 5 wk) after the CP treatment, CD4+-V beta 6+ T cells of C3H origin were preferentially eliminated in the lymph nodes of the tolerant mice, whereas CD8+-V beta 6+ T cells remained. On the other hand, neither CD4+CD8- nor CD4-CD8+ thymocytes bearing a high density of V beta 6 was detected in the chimeric thymus. Namely, in the thymus of the tolerant C3H mice, neither mixed chimerism nor the clonal deletion of the V beta 6-bearing T cells was observed on day 14, whereas both of them were observed on day 35. The clonal deletion and mixed chimerism in the thymus were lasting for greater than 10 wk after the CP treatment. Expression of V beta 6 on the peripheral T cells in the tolerant C3H mice gradually reduced in the process of time. These results strongly suggested that the clonal deletion in the thymus was one of the essential mechanisms in the CP-induced tolerance system.