Miracle-dz/UniFAR-FTUs huggingface dataset:
Strong Manual Acupuncture Manipulation Could Better Inhibit Spike Frequency of the Dorsal Horn Neurons in Rats with Acute Visceral Nociception

Afferent impulses from visceral nociception can be regulated by acupuncture at spinal cord level; however, the effects of different manual acupuncture (MA) manipulations on the afferent impulses are still unknown. Here, we analyzed the spike frequency of excitatory gastric-related wide dynamic range (WDR) neurons in spinal dorsal horn (SDH) following acute gastric distension (GD) in rats and compared their responses to MA manipulations with four different frequencies (0.5, 1, 2, and 3 Hz) at Zusanli (ST36). Results indicated that the spike frequency was increased by acute GD stimulation. Under acute GD circumstances, the spike frequency was further activated by weak MA stimulation (0.5 and 1 Hz), while being significantly inhibited by strong MA stimulation (2 and 3 Hz). After 10 minutes of the strong MA stimulation, same intensity of acute GD caused less spike frequency. Our previous researches had demonstrated that different MA manipulations could increase spike frequency in an intensity-dependent manner in normal rats; these findings suggest that acupuncture may have different modulatory effects depending on the state of the stomach. Since neuronal spike frequency was related to the level of nociception, the results suggest that strong MA manipulation may have better effect on acute visceral nociception.
Phage Display Engineered T Cell Receptors as Tools for the Study of Tumor Peptide–MHC Interactions

Cancer immunotherapy has finally come of age, demonstrated by recent progress in strategies that engage the endogenous adaptive immune response in tumor killing. Occasionally, significant and durable tumor regression has been achieved. A giant leap forward was the demonstration that the pre-existing polyclonal T cell repertoire could be re-directed by use of cloned T cell receptors (TCRs), to obtain a defined tumor-specific pool of T cells. However, the procedure must be performed with caution to avoid deleterious cross-reactivity. Here, the use of engineered soluble TCRs may represent a safer, yet powerful, alternative. There is also a need for deeper understanding of the processes that underlie antigen presentation in disease and homeostasis, how tumor-specific peptides are generated, and how epitope spreading evolves during tumor development. Due to its plasticity, the pivotal interaction where a TCR engages a peptide/MHC (pMHC) also requires closer attention. For this purpose, phage display as a tool to evolve cloned TCRs represents an attractive avenue to generate suitable reagents allowing the study of defined pMHC presentation, TCR engagement, as well as for the discovery of novel therapeutic leads. Here, we highlight important aspects of the current status in this field.
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miniature brain : setting arousal thresholds in a Drosophila Dopamine in References ml#ref-list-1 (414 articles) cognition Articles on similar topics can be found in the following collections Email alerting service here right-hand corner of the article or click Receive free email alerts when new articles cite this article-sign up in the box at the top publication. Citations to Advance online articles must include the digital object identifier (DOIs) and date of initial online articles are citable and establish publication priority; they are indexed by PubMed from initial publication. the paper journal (edited, typeset versions may be posted when available prior to final publication). Advance Advance online articles have been peer reviewed and accepted for publication but have not yet appeared in
ARIA‐suggested drugs for allergic rhinitis: what impact on quality of life? A GA2LEN Review

Allergic diseases constitute a global health problem, as they have an increasing economic and social impact and, especially, they can deeply interfere with the patients’ daily life, being a cause of physical and emotional discomfort. This is why the health‐related quality‐of‐life (HRQoL) has become increasingly important in health care research; in fact, the assessment of the impact the disease and its treatment have on patients, provides a more comprehensive approach in outcome evaluation. Numerous validated questionnaires are available and many studies have been performed evaluating HRQoL in people affected by allergic rhinitis (AR), thus testifying a great interest in this topic. The aims of the present review are: to examine the scientific literature of the last 3 years dealing with the impact of AR treatments suggested by allergic rhinitis and its impact on asthma guidelines on patients’ QoL, and to identify the unexplored or not‐fully‐investigated areas concerning this issue.
Population genomics of the Anthropocene: urbanization is negatively associated with genome‐wide variation in white‐footed mouse populations

Urbanization results in pervasive habitat fragmentation and reduces standing genetic variation through bottlenecks and drift. Loss of genomewide variation may ultimately reduce the evolutionary potential of animal populations experiencing rapidly changing conditions. In this study, we examined genomewide variation among 23 white‐footed mouse (Peromyscus leucopus) populations sampled along an urbanization gradient in the New York City metropolitan area. Genomewide variation was estimated as a proxy for evolutionary potential using more than 10 000 single nucleotide polymorphism (SNP) markers generated by ddRAD‐Seq. We found that genomewide variation is inversely related to urbanization as measured by percent impervious surface cover, and to a lesser extent, human population density. We also report that urbanization results in enhanced genomewide differentiation between populations in cities. There was no pattern of isolation by distance among these populations, but an isolation by resistance model based on impervious surface significantly explained patterns of genetic differentiation. Isolation by environment modeling also indicated that urban populations deviate much more strongly from global allele frequencies than suburban or rural populations. This study is the first to examine loss of genomewide SNP variation along an urban‐to‐rural gradient and quantify urbanization as a driver of population genomic patterns.
Response to Comment on “Oxytocin-mediated GABA inhibition during delivery attenuates autism pathogenesis in rodent offspring”

Bambini-Junior et al. questioned whether our treatment in two rodent models of autism has a long-lasting effect into adulthood. In response, we show that bumetanide treatment around delivery attenuates autistic behavioral features in adult offspring. Therefore, the polarity of γ-aminobutyric acid (GABA) actions during delivery exerts long-lasting priming actions after birth.
Lack of executive functions deficits among adult ad hd individuals from a Brazilian clinical sample

Executive function deficits have been previously documented in individuals with Attention Deficit Hyperactivity Disorder (ADHD). Objective The current study aimed to compare measures of executive functions among a clinical sample of adults with ADHD and normal control subjects, matched for age, gender and education. Methods Twenty-three self-referred adults diagnosed with ADHD according to DSM-IV criteria, and twenty-five control subjects were assessed using a neuropsychological battery which included the Wisconsin Card Sorting Test, Tower of Hanoi, Digit Span, Trail Making Test (A and B), Stroop Test and Raven’s Progressive Matrices. Results The ADHD group did not differ significantly from the control subjects on any of the measures assessed. Conclusion Measures of executive functions using this test battery were unable to discriminate between adults with ADHD and control subjects in this clinical sample.
Critical tunnel cavities for water exclusion in “Green and Ampt” and “Gardner” soils

The limiting case of the analytical solution for the downward flow of water in a Green and Ampt soil around a tunnel cavity of critical shape resting on a permeable substratum at infinite depth gives the shape of the cavity and also the shape of the fringe surface of the surrounding tension‐saturated region as parabolas. The cavity shape is compared with the parabolic shape obtained analytically for the water exclusion from a critical tunnel cavity in a Gardner soil of infinite extent where parabolic isobaric surfaces surround the cavity. The results are used to relate the parameters in the two models. They also add confidence for their use in soil hydrology.
A Simple and Novel Method to Monitor Breathing and Heart Rate in Awake and Urethane-Anesthetized Newborn Rodents

Rodents are most useful models to study physiological and pathophysiological processes in early development, because they are born in a relatively immature state. However, only few techniques are available to monitor non-invasively heart frequency and respiratory rate in neonatal rodents without restraining or hindering access to the animal. Here we describe experimental procedures that allow monitoring of heart frequency by electrocardiography (ECG) and breathing rate with a piezoelectric transducer (PZT) element without hindering access to the animal. These techniques can be easily installed and are used in the present study in unrestrained awake and anesthetized neonatal C57/Bl6 mice and Wistar rats between postnatal day 0 and 7. In line with previous reports from awake rodents we demonstrate that heart rate in rats and mice increases during the first postnatal week. Respiratory frequency did not differ between both species, but heart rate was significantly higher in mice than in rats. Further our data indicate that urethane, an agent that is widely used for anesthesia, induces a hypoventilation in neonates whilst heart rate remains unaffected at a dose of 1 g per kg body weight. Of note, hypoventilation induced by urethane was not detected in rats at postnatal 0/1. To verify the detected hypoventilation we performed blood gas analyses. We detected a respiratory acidosis reflected by a lower pH and elevated level in CO2 tension (pCO2) in both species upon urethane treatment. Furthermore we found that metabolism of urethane is different in P0/1 mice and rats and between P0/1 and P6/7 in both species. Our findings underline the usefulness of monitoring basic cardio-respiratory parameters in neonates during anesthesia. In addition our study gives information on developmental changes in heart and breathing frequency in newborn mice and rats and the effects of urethane in both species during the first postnatal week.
Single-factor repeated-measures designs: analysis and interpretation.

In this column we discussed the selection and interpretation of appropriate statistical tests for single-factor within-subjects/ repeated-measures designs and provided an example from the literature. The parametric tests that we discussed were the t test for paired or correlated samples and the single-factor repeated-measures ANOVA. We also mentioned four nonparametric tests to be used in single-factor within-subjects/repeated-measures designs, but they are relatively rare in the literature. The Compton et al. (2001) article did not provide effect size measures, but they could be computed from the means and standard deviations. Remember that a statistically significant t or ANOVA (even ifp < .001) does not mean that there was a large effect, especially if the sample was large. In the Compton example, the sample was quite small (N = 14), and the findings do reflect a large effect size.
New advances in the management of juvenile idiopathic arthritis—2: The era of biologicals

Juvenile idiopathic arthritis (JIA) is the most common paediatric rheumatic disease with significant long-term morbidity and mortality. Major advances have taken place in recent years in our understanding and the evidence base of JIA. The advent of biological therapies has opened a major new era in the medical management of JIA with recent trials published of etanercept, infliximab, adalimumab, abatacept, tocilizumab and anakinra. National and international collaborative clinical and research networks are ideally placed to enable future advances in the management of JIA and all paediatric rheumatic disorders. This review follows on from Part 1 of a review of recent advances in non-biological therapies in JIA, and focuses on the significant new advances in biological therapies in managing JIA.
Multivariate Regression with Monotone Missing Observation of the Dependent Variables

Multivariate regression is discussed, where the observations of the dependent variables are (monotone) missing completely at random; the explanatory variables are assumed to be completely observed.We discuss OLS-, GLS- and a certain form of E(stimated) GLS-estimation.It turns out that (E)GLS-estimation uses the preceding dependent variables in a well-structured way.In case of normality, ML-estimation coincides with (E)GLS-estimation.We include (sets of) MANOVA-tables enabling us to perform exact tests on the coecients based on a (new) generalized Wilks' distribution.Only the very special case of the constant as sole explanatory variable has been treated in the literature so far: our model incorporates this missing data problem.
Consistent Estimation with a Large Number of Weak Instruments

This paper analyzes the conditions under which consistent estimation can be achieved in instrumental variables (IV) regression when the available instruments are weak and the number of instruments, K n , goes to infinity with the sample size. We show that consistent estimation depends importantly on the strength of the instruments as measured by r n , the rate of growth of the so-called concentration parameter, and also on K n . In particular, when K n →∞, the concentration parameter can grow, even if each individual instrument is only weakly correlated with the endogenous explanatory variables, and consistency of certain estimators can be established under weaker conditions than have previously been assumed in the literature. Hence, the use of many weak instruments may actually improve the performance of certain point estimators. More specifically, we find that the limited information maximum likelihood (LIML) estimator and the bias-corrected two-stage least squares (B2SLS) estimator are consistent when $\sqrt{K_{n}}/r_{n}\rightarrow 0$ K n / r n → 0 , while the two-stage least squares (2SLS) estimator is consistent only if K n /r n →0 as n→∞. These consistency results suggest that LIML and B2SLS are more robust to instrument weakness than 2SLS. Copyright The Econometric Society 2005.
ArchOptions: A Real Options-Based Model for Predicting the Stability of Software Architectures

Architectural stability refers to the extent an architecture is flexible to endure evolutionary changes in stakeholders\' requirements and the environment. We assume that the primary goal of software architecture is to guide the system\'s evolution. We contribute to a novel model that exploits options theory to predict architectural stability. The model is predictive: it provides \"insights\" on the evolution of the software system based on valuing the extent an architecture can endure a set of likely evolutionary changes. The model builds on Black and Scholes financial options theory (Noble Prize wining) to value such extent. We show how we have derived the model: the analogy and assumptions made to reach the model, its formulation, and possible interpretations. We refer to this model as ArchOptions.
Analysis of estimated environments for 2004 and 2005 severe convective storm reports

Analysis of the mesoscale environments associated with observed severe convective storms can provide key insights into their character and predictability, and the challenges to achieving successful severe weather forecasts. The NOAA Storm Prediction Center (SPC) has begun construction of a database of severe storm environments associated with each severe weather report and will analyze these data with the goal of improving national severe weather forecasts. This portion of the broader effort focuses on analysis of estimated environments for all severe convective storms reported during the two year period 2004 and 2005. This manuscript briefly describes the methods used to prepare the database, describes some initial results, and summarizes the efforts to date and the next steps within the broader project.
Severity of Depression and Magnitude of Productivity Loss

PURPOSE Depression is associated with lowered work functioning, including absences, impaired productivity, and decreased job retention. Few studies have examined depression symptoms across a continuum of severity in relationship to the magnitude of work impairment in a large and heterogeneous patient population, however. We assessed the relationship between depression symptom severity and productivity loss among patients initiating treatment for depression. METHODS Data were obtained from patients participating in the DIAMOND (Depression Improvement Across Minnesota: Offering a New Direction) initiative, a statewide quality improvement collaborative to provide enhanced depression care. Patients newly started on antidepressants were surveyed with the Patient Health Questionnaire 9-item screen (PHQ-9), a measure of depression symptom severity; the Work Productivity and Activity Impairment (WPAI) questionnaire, a measure of loss in productivity; and items on health status and demographics. RESULTS We analyzed data from the 771 patients who reported being currently employed. General linear models adjusting for demographics and health status showed a significant linear, monotonic relationship between depression symptom severity and productivity loss: with every 1-point increase in PHQ-9 score, patients experienced an additional mean productivity loss of 1.65% (P <.001). Even minor levels of depression symptoms were associated with decrements in work function. Full-time vs part-time employment status and self-reported fair or poor health vs excellent, very good, or good health were also associated with a loss of productivity (P <.001 and P=.045, respectively). CONCLUSIONS This study shows a relationship between the severity of depression symptoms and work function, and suggests that even minor levels of depression are associated with a loss of productivity. Employers may find it beneficial to invest in effective treatments for depressed employees across the continuum of depression severity.
Influence of ROI selection on resting state functional connectivity: an individualized approach for resting state fMRI analysis

The differences in how our brain is connected are often thought to reflect the differences in our individual personalities and cognitive abilities. Individual differences in brain connectivity has long been recognized in the neuroscience community however it has yet to manifest itself in the methodology of resting state analysis. This is evident as previous studies use the same region of interest (ROIs) for all subjects. In this paper we demonstrate that the use of ROIs which are standardized across individuals leads to inaccurate calculations of functional connectivity. We also show that this problem can be addressed by taking an individualized approach by using subject-specific ROIs. Finally we show that ROI selection can affect the way we interpret our data by showing different changes in functional connectivity with aging.
The impact of a nurse-led care programme on events and physical and psychosocial parameters in patients with heart failure with preserved ejection fraction: A randomized clinical trial in primary care in Russia

Abstract Background: Disease management programmes (DMPs) improve quality of care for patients with heart failure (HF). However, only a limited number of trials have studied the efficacy of such programmes for patients with heart failure with preserved ejection fraction (HFPEF). Objective: To estimate the impact of a structured, nurse-led patient education programme and care plan in general practice on outcome parameters and events in patients with HFPEF. Methods: Single blinded randomized clinical trial with an intervention over six months and a follow-up during 12 additional months. In the control group, the patients (n = 41) were managed according to Russian national guidelines. Patients in the intervention group (n = 44) received education on individual lifestyle changes and modifications of cardiovascular disease (CVD) risk factors, home-based exercise training and weekly nurse consultations in addition to usual care. Results: Six months after their inclusion, patients in the intervention group significantly improved body mass index, waist circumference, six-min walk test distance, total cholesterol, low-density lipoprotein, left ventricular end-diastolic volume index, quality of life and level of anxiety. After 18 months, there were 11 deaths (25%) or hospitalizations in the intervention group and 12 (29%) in the control group (P = 0.134). Cardiovascular mortality and readmission rate were not reduced significantly after six months of follow-up: the hazard ratio was 0.47 (95% CI: 0.17–1.28; P = 0.197). After 18 months, this was 0.85 (0.42–1.73; P = 0.658). Conclusion: This primary care based DMP for patients with HFPEF improved the patients’ emotional status and quality of life, positively influenced body weight, functional capacity and lipid profile, and attenuated heart remodelling.
The Nitric Oxide Donor, O2-Vinyl 1-(Pyrrolidin-1-yl)diazen-1-ium-1,2-diolate (V-PYRRO/NO), Protects against Cadmium-Induced Hepatotoxicity in Mice

The nitric oxide (NO) donor, O2-vinyl 1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate (V-PYRRO/NO), is metabolized by P450 enzymes to release NO within the liver and is effective in protecting against hepatotoxicity of endotoxin and acetaminophen. This study examined the effects of V-PYRRO/NO on cadmium (Cd) hepatotoxicity in mice. Mice were given multiple injections of V-PYRRO/NO (10 mg/kg, s.c. at 2-h intervals) before and after a hepatotoxic dose of Cd (3.7 mg/kg Cd as CdCl2, i.p.). V-PYRRO/NO administration reduced Cd-induced hepatotoxicity as evidenced by reduced serum alanine aminotransferase activity, improved pathology, and reduced hepatic lipid peroxidation. The protection by V-PYRRO/NO was not mediated by altered Cd distribution to the liver or within hepatic subcellular fractions. Similar inductions of metallothionein, a metal-binding protein, were observed in mice receiving Cd alone or Cd plus V-PYRRO/NO. Real-time reverse transcription-polymerase chain reaction analysis revealed that V-PYRRO/NO administration suppressed the expression of inflammation-related genes such as macrophage inflammatory protein-2, CXC chemokine, thrombospondin-1, intracellular adhesion molecular-1, and interleukin-6. V-PYRRO/NO also suppressed the expression of acute phase protein genes and genes related to cell-death pathways, such as c-jun/AP-1, nuclear factor-κB, early response growth factor-1, heme oxygenase-1, caspase-3, growth arrest, and DNA-damaging protein-153. In summary, the liver-selective NO donor, V-PYRRO/NO, protects against Cd hepatotoxicity in mice. This protection is not mediated through altered distribution of Cd but may be related to reduced hepatic inflammation, reduced acute phase responses, and the suppression of cell-death-related components.
Robust image watermarking on the DCT domain

This paper proposes two simple DCT-domain-based schemes to embed single or multiple watermarks into an image for copyright protection and data monitoring and tracking. The watermark data are essentially embedded in the middle band of the DCT domain to make a tradeoff between visual degradation and robustness. The proposed schemes are simple and no original host image is required for watermark extraction. The algorithm also features the capability of embedding multiple orthogonal watermarks into an image simultaneously. A set of systematic experiments, including Gaussian smoothing, JPEG compression, and image cropping are performed to prove the robustness of our algorithms.