Miracle-dz/UniFAR-FTUs huggingface dataset:
The induction of 72-kD gelatinase in T cells upon adhesion to endothelial cells is VCAM-1 dependent

T cell extravasation from the bloodstream into the perivascular tissue during inflammation involves transmigration through the endothelial cell layer and basement membrane into the interstitial matrix. The specific mechanisms by which T cells transmigrate, however, are poorly understood. Matrix degradation by enzymes such as 72-kD gelatinase has been implicated as an important component in tissue invasion by various types of cells. In this study, we have demonstrated that 72-kD gelatinase is induced in T cells upon adhesion to endothelial cells. We also provide evidence that the induction of 72-kD gelatinase is mediated by binding to vascular cell adhesion molecule-1 (VCAM-1). The T cells used in this study were cloned murine Th1 cells antigenic to myelin basic protein. These cells express very late antigen-4 on their cell surface and have been shown to infiltrate the brain parenchyma and cause experimental autoimmune encephalomyelitis when infused into normal mice (Baron, J. L., J. A. Madri, N. H. Ruddle, G. Hashim, and C. A. Janeway. 1993. J. Exp. Med. 177:57-68). In the experiments presented here, T cells were cocultured with VCAM-1-positive and -negative endothelial cells grown in a monolayer in order to study the expression of 72-kD gelatinase upon T cell adhesion. Additional experiments were conducted in which T cells were cocultured with VCAM-1 positive cells grown on microporous membranes suspended in transwells to study 72-kD gelatinase following T cell transmigration. T cells were also incubated with recombinant VCAM-1 in order to study the role of VCAM-1 in inducing 72-kD gelatinase. The results demonstrated that T cells adhered to both VCAM-1-positive and -negative endothelial cells. T cells that adhered to the VCAM-1-positive endothelial cells exhibited an induction in 72-kD gelatinase protein, activity, and mRNA whereas 72- kD gelatinase was not induced in the T cells that adhered to the VCAM-1- negative endothelial cells. Incubating T cells with recombinant VCAM-1 coated onto tissue culture plastic showed that T cells adhered to the molecule and that adhesion to recombinant VCAM-1 was sufficient to induce 72-kD gelatinase. Further, T cells that had transmigrated through a VCAM-1-positive endothelial cell monolayer exhibited 72-kD gelatinase activity but not mRNA expression. In addition, 72-kD gelatinase was detected on the cell surface of the transmigrated T cells by FACS analysis. In other experiments, TIMP-2 was added to the transmigration studies and was shown to reduce T cell transmigration. (ABSTRACT TRUNCATED AT 400 WORDS)
Ventilatory control in newborn mice prenatally exposed to cocaine

Infants born to mothers who used cocaine during pregnancy are at increased risk for neonatal death and respiratory impairments. Confounding factors such as multiple substance abuse make it difficult to isolate the effects of cocaine. We used a murine model to test the hypothesis that prenatal cocaine exposure may impair ventilatory responses to chemical stimuli in newborns. Seventy‐two pregnant mice were randomly assigned to three groups: cocaine (COC), saline (SAL), and untreated (UNT). COC and SAL mice received subcutaneous injections of either 20 mg/kg of cocaine or a saline solution twice a day from gestational days 8–17. Ventilation (V′E) and tidal volume (VT), both divided by body weight, and breath duration (TTOT) were measured using whole‐body plethysmography in freely moving COC (n = 47), SAL (n = 123), and UNT (n = 93) pups on postnatal day 2.
Dividing Attention Between the Hands and the Head: Performance Trade-Offs Between Rapid Finger Tapping and Verbal Memory

We tested a multiple resources approach to time-sharing performance which assumes that each cerebral hemisphere controls its own set of processing resources that it cannot share with the other hemisphere. Right-handed men performed a verbal memory task while concurrently tapping the index finger of either hand as rapidly as possible. Task priority was manipulated with a payoff scheme. Subjects remembered more on the verbal task when concurrently tapping with their left hands than when tapping with their right hands, and their memory performance was much better when the memory task was emphasized than when the tapping task was emphasized, regardless of hand. For the tapping task, decrements from baseline tapping rates and trade-offs between tasks were equal for both hands when subjects were reading the to-be-remembered words aloud. In contrast, during the retention interval, decrements were larger for the right hand than the left, and there were no task trade-offs. The data were interpreted to mean that on right-hand trials, both tasks required exclusively left-hemisphere resources, whereas on left-hand trials, righthemisphere resources were required to execute the tapping movements per se, but left-hemisphere resources were necessary to coordinate those movements with the movements required for overt speech. The data suggest that cognitive and motor resources may be independent types and thus underscore the importance of manipulating task priority to obtain an accurate picture of a task's resource requirements.
Communication as culture: Essays on media and society

Series Editor's Introduction Acknowledgements Introduction PART I: COMMUNICATION AS CULTURE A cultural approach to communication Mass communication and cultural studies Reconceiving 'mass' and 'media' Overcoming resistance to cultural studies PART II: TECHNOLOGY AND CULTURE The mythos of the electronic revolution with John J Quirk Space, time and communications: a tribute to Harold Innis The history of the future with John J Quirk Technology and Ideology: the case of the telegraph Works cited Index About the author
“Inner Voices”

The experience of hearing a “voice” or “loud thoughts” within the head or some other part of the body is not infrequently reported by psychiatric patients. Schneider (1959) has stipulated that “certain modes of hearing voices are of special diagnostic importance for assuming a schizophrenia: hearing one's own thoughts (or thoughts being audible), voices conversing with one another, and voices that keep up a running commentary on the patient's behaviour”. This paper is primarily concerned with the phenomena of “loud” or “audible” thoughts, “inner voices” and similar experiences. It attempts to show that various phenomena superficially resemble the schizophrenic experience of “thoughts becoming audible”, but that distinction is possible on phenomenological grounds.
Writing and the Problem of Will: The Creative Writing Workshop and the Stanley Milgram Paradigm

Abstract The classroom exchange of student feedback, along with the writing exercise and close reading, remains a common staple of Creative Writing teaching. Nevertheless, its acceptance by students rests on a willingness to learn through trial and error, and openness to being guided by their peers and by their tutors along this path. Where these break down, the freedom and informality of the workshop can become the conditions that strengthen hostility and mistrust. At such moments this tutor uses research by Latané and Darley (1970) and Milgram (1974) as material for debate about responsibility, authority and individuality. Their experiments demonstrate that an instinct to conform with their peers, and an instinct to rely on authority figures, interferes with normal people's ability to act as individuals for the common good. In the shorter term, a study of these experiments can help students understand the need to work together under tutorial guidance. In the longer term, they can spur thought and debate on the nature of authority in the artistic calling.
Compromised Rat Testicular Antioxidant Defence System by Hypothyroidism before Puberty

Altered thyroid function during early stages of development is known to affect adversely testicular growth, physiology, and antioxidant defence status at adulthood. The objective of the present study is to investigate the modulation of antioxidant defence status in neonatal persistent hypothyroid rats before their sexual maturation and also to identify the specific testicular cell populations vulnerable to degeneration during neonatal hypothyroidism in immature rats. Hypothyroidism was induced in neonates by feeding the lactating mother with 0.05% 6-n-propyl-2-thiouracil (PTU) through the drinking water. From the day of parturition till weaning (25 day postpartum), the pups received PTU through mother's milk (or) drinking water and then directly from drinking water containing PTU for the remaining period of experimentation. On the 31st day postpartum, the animals were sacrificed for the study. An altered antioxidant defence system marked by elevated SOD, CAT, and GR activities, with decreased GPx and GST activities were observed along with increased protein carbonylation, disturbed redox status in hypothyroid immature rat testis. This compromised testicular antioxidant status might have contributed to poor growth and development by affecting the spermatogenesis and steroidogenesis in rats before puberty as indicated by reduced germ cell number, complete absence of round spermatids, decreased seminiferous tubule diameter, and decreased testosterone level.
ADULTHOOD AND REMINISCENCE: A DESCRIPTIVE STUDY

Abstract Although reminiscence is a behavior common to all ages, research to date has been conducted only with older adults. This study sought to explore the phenomenon with adults of various ages with the anticipation that comparative information would lead to a better conceptualization of the behavior and its functions. It was hypothesized that there would be no difference in what adults reminisced about, why they reminisced, or how they reminisced with respect to age, sex, education, or socioeconomic status. A sample of 309 adults ranging in age from 18 to 90 were asked to complete a survey exploring various aspects of reminiscence. Analysis of variance and chi‐square were used to assess differences in reminiscent behavior across age, sex, education, and social class. There were no systematic differences with regard to education or social class. On several dimensions sex emerged as a more important variable than age, suggesting that age may not be as important a consideration in simple reminiscence as .
Alternative Teacher Certification: Its Policy Implications for Classroom and Personnel Practice

Alternative certification (AC) of teachers is an issue of interest and discussion within the 1980s education reform movement. Little empirical evaluation is available for formulating policy, however. This research describes an alternative certification program in the Dallas Independent School District. Recruitment programs resulted in 4,000 inquiries and over 1,300 applicants, 691 of whom took the entrance test, 557 who passed, and 110 who were admitted to the program. This research addresses eight topics: (a) characteristics of the program, (b) characteristics of the interns, (c) intern attitudes, (d) comparison of interns and traditionally certified teachers, (e) teaching performance of interns, (f) predictors of AC success, (g) reaction to the program, and (h) consideration of whether AC programs can contribute to the need for teachers. Policy recommendations, based on the above, are formulated.
Safer Sex Stories Told by Young Gay Men: Building on Resiliency Through Gay-Boy Talk

ABSTRACT Most research about HIV among gay youth focuses on HIV risk behaviors. Many of the studies of gay youth are also driven by a deficit model of development. Scholars have argued that such deficit models may be contributing to their stigmatization while ignoring their resiliency. This article focuses attention on a subset of the sexual stories told by young gay men as part of the Sexual Stores Project. The twenty Anglo and twenty Latino young gay men, ages 18–24, were drawn using a snowball sampling procedure within each ethnic group. Analyses of these semi-structured interviews allow for examinations of these young gay men's safe sex accounts and their experiences in sex education at school. Recommendations for meaningful and relevant HIV prevention sex education guided by “Gay-boy” talk are provided.
Ionotropic GABA Receptors and Distal Retinal ON and OFF Responses

In the vertebrate retina, visual signals are segregated into parallel ON and OFF pathways, which provide information for light increments and decrements. The segregation is first evident at the level of the ON and OFF bipolar cells in distal retina. The activity of large populations of ON and OFF bipolar cells is reflected in the b- and d-waves of the diffuse electroretinogram (ERG). The role of gamma-aminobutyric acid (GABA), acting through ionotropic GABA receptors in shaping the ON and OFF responses in distal retina, is a matter of debate. This review summarized current knowledge about the types of the GABAergic neurons and ionotropic GABA receptors in the retina as well as the effects of GABA and specific GABAA and GABAC receptor antagonists on the activity of the ON and OFF bipolar cells in both nonmammalian and mammalian retina. Special emphasis is put on the effects on b- and d-waves of the ERG as a useful tool for assessment of the overall function of distal retinal ON and OFF channels. The role of GABAergic system in establishing the ON-OFF asymmetry concerning the time course and absolute and relative sensitivity of the ERG responses under different conditions of light adaptation in amphibian retina is also discussed.
Bioactivity as an Options Value of Sea Cucumbers in the Egyptian Red Sea

Abstract: The utility of a species can be divided into its direct, indirect, and options values. In the marine environment, direct consumptive values predominate and often lead to overexploitation at the expense of significant options values derived through bioprospecting for natural products. We surveyed the waters of the Egyptian Red Sea coast (Gulf of Aqaba [north] and the Red Sea [south]) for species of sea cucumbers and analyzed extracts from species for a range of bioactivities with potential biomedical applications. All habitat types were surveyed within these regions. We found 22 species of sea cucumber of which two, Holothuria fuscogilva and Holothuria flavomaculata, were recorded in Egypt for the first time. Although none of the species identified were unique to the Gulf of Aqaba, 10 species were only found in the Red Sea sector. Bioassay results showed that although no species had antibacterial activity, most extracts exhibited activity against Candida and Leishmania but were most active against a LoVo mammalian carcinoma cell line. Our most significant finding was the intraspecific variation in bioactivity in individuals collected from different habitat types and sectors of the coast. This variation may reflect the effect of environment on secondary metabolite production or may indicate significant genetic diversity between populations within a species. Our results indicate a potentially significant options value to sea cucumbers through bioprospecting. Given the importance of economic development in countries such as Egypt and the perceived low conservation value of invertebrates such as sea cucumbers, the linking of these factors to conservation is vital for the maintenance and sustainable exploitation of these animals.
The ERCC1 C118T polymorphism predicts clinical outcomes of colorectal cancer patients receiving oxaliplatin-based chemotherapy: a meta-analysis based on 22 studies.

BACKGROUND Although the predictive value of the excision repair cross-complementing group 1 (ERCC1) C118T polymorphism in clinical outcomes of patients with colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy has been evaluated in numerous published studies, the conclusions are conflicting. Therefore, we performed the present meta-analysis to determine the precise role of the ERCC1 C118T polymorphism in this clinical situation and help optimize individual chemotherapy. MATERIALS AND METHODS A multiple search strategy was used to identify eligible studies. Pooled odds ratios (ORs) and their 95% confidence intervals (CIs) were used to estimate objective response and oxaliplatin-induced toxicity, with hazard ratios (HRs) with 95%CIs for progression-free survival (PFS) and overall survival (OS). RESULTS A total of 22 studies including 2,846 CRC patients were eligible in the analysis. Overall, no significant correlation was found between the ERCC1 C118T polymorphism and objective response to oxaliplatin-based chemotherapy, in all patients or in the Asian and Caucasian subgroups. However, the pooled analysis showed that the PFS and OS were significantly shorter in patients who carried T/T or T/C genotypes of ERCC1 C118T as compared to the C/C genotype. On stratified analysis by ethnicity, the ERCC1 118T allele was associated with a favorable prognosis in Caucasians (PFS, HR=0.58, 95%CI: 0.24-1.44; OS, HR=0.38, 95%CI: 0.22-0.64) but an unfavorable prognosis in Asians (PFS, HR=2.49, 95%CI: 1.87-3.33; OS, HR=2.63, 95%CI: 1.87-3.69) based on a dominant model. In addition, we failed to find a statistically significant impact of ERCC1 C118T polymorphism on oxaliplatin-induced toxicity. CONCLUSIONS The ERCC1 C118T polymorphism may have prognostic value in patients with CRC undergoing oxaliplatin-based chemotherapy.
Body, Psyche, and Culture: The Relationship between Disgust and Morality

"Core disgust" is a food related emotion that is rooted in evolution but is also a cultural product. Seven categories of disgust elicitors have been observed in an American sample. These include food, animals, body products, sexual de viance, body-envelope violations, poor hygiene, and contact with death. In addition, social concerns such as interpersonal contamination and socio- moral violations are also associated with disgust. Cross-cultural analyses of disgust and its elicitors using Israeli, Japanese, Greek and Hopi notions of disgust were undertaken. It was noted that disgust elicitors have expanded from food to the social order and have been found in many cultures. Expla nations for this expansion are provided in terms of embodied schemata, which refer to imaginative structures or patterns of experience that are based on bodily knowledge or sensation. A mechanism is suggested whereby disgust elicitors are viewed as a prototypically defined category involving many of the embodied schemata of disgust. It is argued that each culture draws upon these schemata and its social and moral life is based on them.
Impact of aerobic exercise trials with varied intensity patterns on perceptions of effort: An evaluation of predicted, in-task, and session exertion

Abstract Perceptions of effort provide a sense of momentary exertion during exercise, but may have value when considered prior to and after exercise. The purpose of the present study was to determine the relationship between perceived exertion assessed before, during, and after trials of aerobic exercise fixed for total work but varied on intensity patterns. Twenty participants completed five trials of treadmill exercise that included planned intensity variations every 10 minutes to result in the same total work. Perceived exertion was taken before, during, and after exercise. Analyses confirmed each trial was similar in terms of average speed and heart rate (P > 0.05). Predicted and session exertion results indicated that the steady exercise bout was perceived as easier than many trials of varied speed (P < 0.05). Trials including an intense segment in the middle or end of the session were perceived as more difficult than sessions that varied intensity otherwise or remained constant. Similarly, trials that remained constant during the 30-minute period were perceived as less aversive than most other trials. These findings suggest that exercise sessions involving a steady intensity throughout are viewed as less taxing than sessions with variable intensity, especially when compared to sessions that conclude with a higher intensity finish.
Active and passive immunisation against respiratory syncytial virus

RSV is a major cause of respiratory illness in infants under 2 years of age. Evidence is accumulating that it is also underestimated as a cause of respiratory infection in adults, the elderly and immunocompromised individuals. Active interventions to control the impact of RSV infection have been hampered by a lack of understanding of the immune response to RSV in different age groups. A number of different strategies for developing RSV vaccines have been pursued, including live attenuated vaccines, genetically engineered live and subunit vaccines and peptide vaccines with varying degrees of success. The target populations for RSV vaccines include infants, the elderly and women of childbearing age, but the efficacy of different vaccines may differ according to age. Desirable immune responses and immune correlates of protection to RSV in humans remain uncertain and determining these is critical for introduction of any vaccines.
Pain over time and its effects on life in thalassemia

Pain reports have become increasingly common and problematic in thalassemia. As patients are living longer, there is a growing need to study pain and to explore its impact on patient lives. The Brief Pain Inventory (BPI) was used quarterly to assess pain and pain interference in patients with thalassemia in North America. The Medical Outcomes Study 36‐Item Short Form Health Survey and the Hospital Anxiety and Depression Scale were used to assess quality of life, anxiety and depression. Of the 252 participants, 56% reported pain at least once over the course of this study, with 32% reporting severe pain (≥7/10); 16% reported pain at all four visits. Increased pain severity significantly interfered with daily life (P < 0.001; regression analysis) and participants with more sites of pain showed an increase in the amount of daily activities affected by pain (P = 0.001). Participants reporting more visits with pain reported a significantly higher impact on affective and physical function (P < 0.001). Physical quality of life decreased with increasing numbers of visits with pain (P < 0.001). Those who reported one or more sites of pain showed increased symptoms of both depression (P < 0.001) and anxiety (P = 0.003). Participants reporting at least two visits with pain had higher symptoms of anxiety (P = 0.002) and those with at least three visits reported higher symptoms of depression (P = 0.003). Pain is prevalent in thalassemia and is often a chronic condition that interferes with life. The study highlights the significance of pain in thalassemia and its impact, which should be considered in future research and treatments. Am. J. Heamtol. 88:939–943, 2013. © 2013 Wiley Periodicals, Inc.
Why Do Household Portfolio Shares Rise in Wealth?

We develop a life-cycle consumption and portfolio choice model in which households have nonhomothetic utility over two types of goods, basic and luxury. We calibrate the model to match the cross-sectional and life-cycle variation in the basic expenditure share in the Consumer Expenditure Survey. The model explains the degree to which the portfolio share in risky assets rises in wealth in the cross-section of households in the Survey of Consumer Finances. For a given household, the portfolio share can fall in response to an increase in wealth, even though the model implies decreasing relative risk aversion.