Miracle-dz/UniFAR-FTUs huggingface dataset:
Increased Platelet and Microparticle Activation in HIV Infection: Upregulation of P-Selectin and Tissue Factor Expression

Objective:HIV-1–infected patients have an increased risk for atherothrombosis and cardiovascular disease, but the mechanism behind these risks is poorly understood. We have previously reported that expression of tissue factor (TF) on circulating monocytes is increased in persons with HIV infection and that TF expression is related to immune activation, to levels of HIV in plasma, and to indices of microbial translocation. In this study, we explore the activation state of platelets in HIV disease. Methods:Here, using flow cytometry-based assays, we measured platelet and platelet microparticle (PMP) activation in samples from HIV-1–infected donors and controls. Results:Platelets and PMPs from HIV-1–infected patients are activated (as reflected by expression of CD62 P-selectin) and also more frequently expressed the procoagulant TF than did platelets and PMPs obtained from controls. Expression of these proteins was directly related to expression of TF on monocytes, to markers of T-cell activation (CD38 and HLA-DR), and to plasma levels of soluble CD14, the coreceptor for bacterial lipopolysaccharride. Platelet and microparticle expression of TF was not related to plasma levels of HIV but expression of P-selectin was related to plasma levels of HIV; neither TF nor P-selectin expression was related to CD4 T-cell count. Conclusions:Platelets and microparticles are activated in HIV infection, and this activated phenotype may contribute to the increased risk for cardiovascular and thrombotic events in this population although a role for other confounding cardiovascular risks cannot be completely excluded.
Effects of polymer/metal interaction in thin-film multichip module applications

An investigation was conducted to examine the effects of different polyimide/metal combinations on structures similar to those used in thin-film multichip module applications. Three different metal structures and three commercially available spin-on polyimide materials which possess different molecular structures were examined. Transmission electron microscopy was used to study the polyimide/metal interfaces. Measurements of the transmission characteristics of line structures fabricated using the different metal/polyimide combinations were compared. It was found that the extent of interaction of polyimide with copper is dependent upon the type of polyimide used. Dupont 2611D polyimide showed the least amount of interaction with only a small band of precipitates present approximately 5 kAA from the copper/polyimide interface, which do not affect the electrical transmission characteristics of lines spaced 22 mu m a part. The use of an electrodes nickel layer on copper to retard copper/polyimide interaction from occurring during the curing cycle of the polyimide was found to be effective. However, increased loss of transmission at high frequencies and narrow spacing was seen for nickel-clad lines embedded in Dupont 2611D polyimide. Since nickel is a magnetic material, this increase in loss may be due to natural inductance from the nickel present in neighboring lines. <<ETX>>
Toward Energy-Efficient 5G Wireless Communications Technologies: Tools for decoupling the scaling of networks from the growth of operating power

The densification and expansion of wireless networks pose new challenges on energy efficiency. With a drastic increase of infrastructure nodes (e.g. ultradense deployment of small cells), the total energy consumption may easily exceed an acceptable level. While most studies focus on the energy radiated by the antennas, the bigger part of the total energy budget is actually consumed by the hardware (e.g., coolers and circuit energy consumption). The ability to shut down infrastructure nodes (or parts of it) or to adapt the transmission strategy according to the traffic will therefore become an important design aspect of energy-efficient wireless ? architectures. Network infrastructure should be ? regarded as a resource that can be occupied or released on demand, and the modeling and optimization of such systems are highly nontrivial problems. In particular, elements of the network infrastructure should be released by taking into account traffic forecasts to avoid losing the required coverage and capacity. However, even if traffic profiles were perfectly known, the determination of the elements to be released is complicated by the potential interference coupling between active elements and the sheer size of the optimization problems in dense networks.
Exploring the Anti-Cancer Activity of Novel Thiosemicarbazones Generated through the Combination of Retro-Fragments: Dissection of Critical Structure-Activity Relationships

Thiosemicarbazones (TSCs) are an interesting class of ligands that show a diverse range of biological activity, including anti-fungal, anti-viral and anti-cancer effects. Our previous studies have demonstrated the potent in vivo anti-tumor activity of novel TSCs and their ability to overcome resistance to clinically used chemotherapeutics. In the current study, 35 novel TSCs of 6 different classes were designed using a combination of retro-fragments that appear in other TSCs. Additionally, di-substitution at the terminal N4 atom, which was previously identified to be critical for potent anti-cancer activity, was preserved through the incorporation of an N4-based piperazine or morpholine ring. The anti-proliferative activity of the novel TSCs were examined in a variety of cancer and normal cell-types. In particular, compounds 1d and 3c demonstrated the greatest promise as anti-cancer agents with potent and selective anti-proliferative activity. Structure-activity relationship studies revealed that the chelators that utilized “soft” donor atoms, such as nitrogen and sulfur, resulted in potent anti-cancer activity. Indeed, the N,N,S donor atom set was crucial for the formation of redox active iron complexes that were able to mediate the oxidation of ascorbate. This further highlights the important role of reactive oxygen species generation in mediating potent anti-cancer activity. Significantly, this study identified the potent and selective anti-cancer activity of 1d and 3c that warrants further examination.
Outcome after in-patient detoxification for alcohol dependence: a naturalistic comparison of 7 versus 28 days stay.

Research has tended to show that the gains of residential rehabilitation are short-term and cost-inefficient. This study compares the outcomes of two samples, one group staying at a non-statutory sector alcohol detoxification unit for < or =7 days (short stay: SS) with a second group also admitted for detoxification but who stayed at the Unit for a further 8-21 days (long stay: LS). Allocation was not at random: the longer stay was either at the request of the client, referring or treatment agency itself and then had to be approved by an external funding agency. Sixty-four DSM-IV (Diagnostic and Statistical Manual of Mental Disorders) alcohol-dependent subjects were studied. Baseline data included socio-demographic information, illicit drug use during the past 12 months, severity of alcohol dependence, alcohol problems, physical/psychological symptoms, depression and indices of quality of life. At baseline, LS subjects reported more recreational cannabis use than SS subjects. Sixty-two (97%) subjects were re-interviewed 12 weeks after baseline assessment. During follow-up, equal proportions of each group relapsed (> or =21 units/7 day period fo males; > or =14 units/7day period for females). There was a trend for SS clients to have consumed less alcohol in total than the LS clients. The trend was towards improvement in the study measurements for the SS group, though none of the changes was significant. In the LS group, all variables tended towards a deterioration in health status. The longer stay did not appear to confer any extra benefit to the LS group. Cannabis use and illicit drug use at baseline, while commoner in the LS group, did not predict drinking or social adjustment in the follow-up period in this sample and thus could not be used to explain the lack of a better outcome in the LS group.
Seiberg-Witten Curve for the E-String Theory

We construct the Seiberg-Witten curve for the E-string theory in six-dimensions. The curve is expressed in terms of affine E8 characters up to level 6 and is determined by using the mirror-type transformation so that it reproduces the number of holomorphic curves in the Calabi-Yau manifold and the amplitudes of = 4 U(n) Yang-Mills theory on ½K3. We also show that our curve flows to known five- and four-dimensional Seiberg-Witten curves in suitable limits.
Downregulation of oxytocin receptors in right ventricle of rats with monocrotaline‐induced pulmonary hypertension

Aim: Pulmonary hypertension (PH) in the rat leads to right ventricular (RV) hypertrophy, inflammation and increased natriuretic peptide (NP) levels in plasma and RV. Because the release of nitric oxide (NO) and atrial natriuretic peptide (ANP) is a function of the oxytocin receptor (OTR), we examined the effect of PH on gene and protein expression of OTR, NP (A, atrial; B, brain) and receptors (NPRs), nitric oxide synthases (NOS), interleukin (IL)‐1β, IL‐6 and tumour necrosis factor‐α in the hypertrophied RV in a model of PH.
New Approaches to Volcanic Time Series Analysis

The literature on geophysical time series analysis is so extensive that to review even one topic, such as volcanic tremor series, is a major task (e.g. Konstantinou and Schlindwein 2002). The purpose of this chapter is not to attempt such a review but rather to outline some new tools for nonstationary and nonlinear time series analysis that have been developed and used successfully in other areas of the environmental sciences and appear to have good potential for application in a volcanological or wider geophysical context. These stochastic methods of time series analysis have the advantage that they all exploit powerful recursive (sequential updating) methods of estimation that facilitate the analysis of data generated by nonstationary and nonlinear systems (e.g. Young 1984). This Chapter starts by reviewing briefly some of the model-based methods of time series analysis, signal extraction and forecasting that have appeared in the statistical and time series analysis literature over many years and then proceeds to describe in more detail one approach that has attracted considerable interest over the past two decades. This is based on the concept of an ‘unobserved component’ model and it exploits recursive estimation for the purposes of estimating time variable parameters in nonstationary systems. It is shown that such an approach allows for various, practically useful procedures in time series analysis: signal extraction; interpolation over gaps in time series; and forecasting or backcasting. It then goes on to outline the basic aspects of input-output time series modelling, considering both discrete-time and continuous-time ‘transfer function’ models that are
CT Diagnosis of Appendicitis in Children: Comparison of Orthogonal Planes and Assessment of Contrast Opacification of the Appendix

Objective The aim of this study was to determine which plane is best for identification of the appendix and to assess if opacification of the appendix impacts visualization. Methods Retrospective review of 218 computed tomography examinations performed for suspected appendicitis was conducted by 2 pediatric radiologists evaluating conspicuity of the appendix depending on orthogonal plane and enteric contrast. Results Of the 180 cases in which the appendix was visualized, 154 were performed with multiplanar reformations. The best plane for identification of the appendix was coronal in 96, axial in 41, and sagittal in 17. Of the 218 computed tomography examinations, 169 had enteric contrast. The appendix was identified in 180 cases and completely opacified in 62, partially opacified in 26, without opacification in 59, and air filled in 33. Of the 38 cases in which the appendix was not identified, 29 had enteric contrast administration. In 131 of the 218 cases, the appendix was normal, and 58% demonstrated partial or complete opacification of the appendix. In 35 of the 218 cases, appendicitis was found, and there was predominantly no opacification or at most partial opacification of the appendix. Conclusions The coronal plane is best to identify the appendix. No enteric contrast is required to visualize appendicitis.
High stability of self‐assembled peptide nanowires against thermal, chemical, and proteolytic attacks

Understanding the self‐assembly of peptides into ordered nanostructures is recently getting much attention since it can provide an alternative route for fabricating novel bio‐inspired materials. In order to realize the potential of the peptide‐based nanofabrication technology, however, more information is needed regarding the integrity or stability of peptide nanostructures under the process conditions encountered in their applications. In this study, we investigated the stability of self‐assembled peptide nanowires (PNWs) and nanotubes (PNTs) against thermal, chemical, proteolytic attacks, and their conformational changes upon heat treatment. PNWs and PNTs were grown by the self‐assembly of diphenylalanine (Phe–Phe), a peptide building block, on solid substrates at different chemical atmospheres and temperatures. The incubation of diphenylalanine under aniline vapor at 150°C led to the formation of PNWs, while its incubation with water vapor at 25°C produced PNTs. We analyzed the stability of peptide nanostructures using multiple tools, such as electron microscopy, thermal analysis tools, circular dichroism, and Fourier‐transform infrared spectroscopy. Our results show that PNWs are highly stable up to 200°C and remain unchanged when incubated in aqueous solutions (from pH 1 to 14) or in various chemical solvents (from polar to non‐polar). In contrast, PNTs started to disintegrate even at 100°C and underwent a conformational change at an elevated temperature. When we further studied their resistance to a proteolytic environment, we discovered that PNWs kept their initial structure while PNTs fully disintegrated. We found that the high stability of PNWs originates from their predominant β‐sheet conformation and the conformational change of diphenylalanine nanostructures. Our study suggests that self‐assembled PNWs are suitable for future nano‐scale applications requiring harsh processing conditions. Biotechnol. Bioeng. 2010; 105: 221–230. © 2009 Wiley Periodicals, Inc.
Subsidies in Oligopoly Markets: a Welfare Comparison Between Symmetric and Asymmetric Costs

This article studies welfare effects of uniform production subsidies in oligopoly markets, comparing cases of symmetric and asymmetric costs. Cost asymmetry reduces the welfare impact relative to the symmetric-cost case if the demand function is concave and magnifies the impact if demand is convex. The welfare difference increases with the degree of market power and with the cost differential in the industry.
Intratumoral chemotherapy for lung cancer: re-challenge current targeted therapies

Strategies to enhance the already established doublet chemotherapy regimen for lung cancer have been investigated for more than 20 years. Initially, the concept was to administer chemotherapy drugs locally to the tumor site for efficient diffusion through passive transport within the tumor. Recent advances have enhanced the diffusion of pharmaceuticals through active transport by using pharmaceuticals designed to target the genome of tumors. In the present study, five patients with non-small cell lung cancer epidermal growth factor receptor (EGFR) negative stage IIIa–IV International Union Against Cancer 7 (UICC-7), and with Eastern Cooperative Oncology Group (ECOG) 2 scores were administered platinum-based doublet chemotherapy using combined intratumoral-regional and intravenous route of administration. Cisplatin analogues were injected at 0.5%–1% concentration within the tumor lesion and proven malignant lymph nodes according to pretreatment histological/cytological results and the concentration of systemic infusion was decreased to 70% of a standard protocol. This combined intravenous plus intratumoral-regional chemotherapy is used as a first line therapy on this short series of patients. To the best of our knowledge this is the first report of direct treatment of involved lymph nodes with cisplatin by endobronchial ultrasound drug delivery with a needle without any adverse effects. The initial overall survival and local response are suggestive of a better efficacy compared to established doublet cisplatin–based systemic chemotherapy in (higher) standard concentrations alone according to the UICC 7 database expected survival. An extensive search of the literature was performed to gather information of previously published literature of intratumoral chemo-drug administration and formulation for this treatment modality. Our study shows a favorable local response, more than a 50% reduction, for a massive tumor mass after administration of five sessions of intratumoral chemotherapy plus two cycles of low-dose intravenous chemotherapy according to our protocol. These encouraging results (even in very sick ECOG 2 patients with central obstructive non-small cell lung cancer having a worse prognosis and quality of life than a non-small cell lung cancer in ECOG 0 of the same tumor node metastasis [TNM]-stage without central obstruction) for a chemotherapy-only protocol that differs from conventional cisplatin-based doublet chemotherapy by the route, target site, and dose paves the way for broader applications of this technique. Finally, future perspectives of this treatment and pharmaceutical design for intratumoral administration are presented.
Distinct afferent innervation patterns within the human proximal and distal esophageal mucosa

Little is known about the mucosal phenotype of the proximal human esophagus. There is evidence to suggest that the proximal esophagus is more sensitive to chemical and mechanical stimulation compared with the distal. This may have physiological relevance (e.g., in prevention of aspiration of gastroesophageal refluxate), but also pathological relevance (e.g., in reflux perception or dysphagia). Reasons for this increased sensitivity are unclear but may include impairment in mucosal barrier integrity or changes in sensory innervation. We assessed mucosal barrier integrity and afferent nerve distribution in the proximal and distal esophagus of healthy human volunteers. In 10 healthy volunteers baseline proximal and distal esophageal impedance was measured in vivo. Esophageal mucosal biopsies from the distal and proximal esophagus were taken, and baseline transepithelial electrical resistance (TER) was measured in Ussing chambers. Biopsies were examined immunohistochemically for presence and location of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers. In a further four healthy volunteers we investigated for colocalization of CGRP and protein gene product (PGP) 9.5 immunoreactivity in nerve fibers. Baseline impedance was higher in the proximal than in the distal esophagus [2,936 Ω (SD578) vs. 2,229 Ω (SD821); P = 0.03], however, baseline TER was not significantly different between them. Mucosal CGRP-immunoreactive nerves were found in the epithelium of both proximal and distal esophagus, but were located more superficially in the proximal mucosa compared with the distal [11.5 (SD7) vs. 21.7 (SD5) cell layers from lumen, P = 0.002] 19% of proximal, and 10% of distal mucosal PGP-immunoreactive fibers colocalized with CGRP. PGP-immunoreactive fibers were also significantly closer to the luminal surface in the proximal compared with the distal esophagus (P < 0.001). We conclude that mucosal barrier integrity is similar in proximal and distal esophagus, but proximal mucosal afferent nerves are in a more superficial location. The enhanced sensitivity to reflux-evoked symptoms of the proximal esophagus most likely has an anatomical basis.
Comparative Genome Analysis of Lactobacillus rhamnosus Clinical Isolates from Initial Stages of Dental Pulp Infection: Identification of a New Exopolysaccharide Cluster

The human oral microbiome has a major role in oral diseases including dental caries. Our studies on progression of caries infection through dentin and more recently, the invasion of vital dental pulp, detected Lactobacillus rhamnosus in the initial stages of infection of vital pulp tissue. In this study employing current high-throughput next generation sequencing technology we sought to obtain insight into genomic traits of tissue invasive L. rhamnosus, to recognise biomarkers that could provide an understanding of pathogenic potential of lactobacilli, generally regarded as safe. Roche GS FLX+ technology was used to generate whole genome sequences of two clinical isolates of L. rhamnosus infecting vital pulp. Detailed genome-wide comparison of the genetic profiles of tissue invasive L. rhamnosus with probiotic L. rhamnosus was performed to test the hypothesis that specific strains of L. rhamnosus possessing a unique gene complement are selected for the capacity to invade vital pulp tissue. Analysis identified 264 and 258 genes respectively, from dental pulp-invasive L. rhamnosus strains LRHMDP2 and LRHMDP3 isolated from two different subjects that were not present in the reference probiotic L. rhamnosus strain ATCC 53103 (GG). Distinct genome signatures identified included the presence of a modified exopolysaccharide cluster, a characteristic confirmed in a further six clinical isolates. Additional features of LRHMDP2 and LRHMDP3 were altered transcriptional regulators from RpoN, NtrC, MutR, ArsR and zinc-binding Cro/CI families, as well as changes in the two-component sensor kinase response regulator and ABC transporters for ferric iron. Both clinical isolates of L. rhamnosus contained a single SpaFED cluster, as in L. rhamnosus Lc705, instead of the two Spa clusters (SpaCBA and SpaFED) identified in L. rhamnosus ATCC 53103 (GG). Genomic distance analysis and SNP divergence confirmed a close relationship of the clinical isolates but segregation from the reference probiotic L. rhamnosus strain ATCC 53103 (GG).
A review of validated youth prevention and positive youth development programs in Asia

Abstract In view of the intensification of adolescent developmental issues in different Asian contexts, there is an urgent need for developing prevention and positive youth development programs in different Asian communities. In this paper, adolescent prevention and positive youth development programs in Asia which have been evaluated by studies adopting true experimental or quasi-experimental designs are reviewed. Several observations are highlighted from this review. First, compared with Western societies, the number of validated programs in different Asian communities was extremely low. Second, there were comparatively more programs addressing substance abuse than other mental health problems. Third, compared with evaluated prevention programs, there were very few positive youth development programs. Finally, there were very few rigorously designed evaluative studies of prevention and positive youth development programs over a long period of time.