Miracle-dz/UniFAR-FTUs huggingface dataset:
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity

In obesity, white adipose tissue (WAT) inflammation is linked to insulin resistance. Increased adipocyte chemokine (C-C motif) ligand 2 (CCL2) secretion may initiate adipose inflammation by attracting the migration of inflammatory cells into the tissue. Using an unbiased approach, we identified adipose microRNAs (miRNAs) that are dysregulated in human obesity and assessed their possible role in controlling CCL2 production. In subcutaneous WAT obtained from 56 subjects, 11 miRNAs were present in all subjects and downregulated in obesity. Of these, 10 affected adipocyte CCL2 secretion in vitro and for 2 miRNAs (miR-126 and miR-193b), regulatory circuits were defined. While miR-126 bound directly to the 3′-untranslated region of CCL2 mRNA, miR-193b regulated CCL2 production indirectly through a network of transcription factors, many of which have been identified in other inflammatory conditions. In addition, overexpression of miR-193b and miR-126 in a human monocyte/macrophage cell line attenuated CCL2 production. The levels of the two miRNAs in subcutaneous WAT were significantly associated with CCL2 secretion (miR-193b) and expression of integrin, α-X, an inflammatory macrophage marker (miR-193b and miR-126). Taken together, our data suggest that miRNAs may be important regulators of adipose inflammation through their effects on CCL2 release from human adipocytes and macrophages.
Reconsidering Intrinsic Religion as a Source of Universal Compassion

Several studies have considered the ability of devout Christians to accept homosexual persons while rejecting homosexual behavior. Batson, Floyd, Meyer, and Winner (1999) found that intrinsic Christians were not able to make that distinction. Bassett et al. (2000) found that intrinsic Christians were able to distinguish between the value of homosexual persons and behavior. Both studies used financial help as the measure of acceptance. This study replicated the Batson et al. methodology with some modifications. The basic methodology involves students working at a time-limited task, which might financially benefit another “student. ” What is varied in the procedure is the description of the other “student” (regarding sexual orientation and what the person intends to do with the money). Results from the study suggested that intrinsic Christians were able to “love the sinner but hate the sin. ”
The Single and Combined Effects of Multiple Intensities of Behavior Modification and Methylphenidate for Children With Attention Deficit Hyperactivity Disorder in a Classroom Setting

Currently behavior modification, stimulant medication, and combined treatments are supported as evidence-based interventions for attention deficit hyperactivity disorder in classroom settings. However, there has been little study of the relative effects of these two modalities and their combination in classrooms. Using a within-subject design, the present study investigated the single effects of behavior modification (no, low, and high intensity), methylphenidate (placebo, 0.15, 0.30, and 0.60 mg/kg), and the combination of these treatments in an analogue classroom setting with 48 children (ages 6–12) diagnosed with attention deficit hyperactivity disorder. Results indicated substantial effects of both unimodal treatments and their combination on measures of classroom behavior, productivity, and teacher ratings of functioning. The results are discussed in light of the current literature on single and combined treatments for attention deficit hyperactivity disorder.
Ethics in the Digital Age: A Comparison of the Effects of Moving Images and Photographs on Moral Judgment

This study used a controlled experiment to see if moving images can improve moral judgment the same way that still images have in previous research. It found that watching a video only once caused participants' reasoning about ethical issues to decline significantly compared with those who saw a still photograph. This adds to the mounting evidence that still images elevate the quality of reasons used to make ethical decisions. The media have the power to not only set the public's agenda but also to shape ethical decision making regarding how citizens solve problems in society. In order to help audiences think more ethically about issues in the news, using still photographs is better than showing video, especially when stories are ethically charged, and improved ethical thinking can help achieve a more just society.
Word meaning, cognitive development, and social interaction

This review proposes that Bloom's linkage of word meaning with more general cognitive capacities could be extended through examination of the social contexts in which children learn. Specifically, the child's developing theory of mind can be viewed as part of the process by which children learn word meanings through engagement in social interactions that facilitate both language and strategic behaviours.
Effect of Human Milk Appetite Hormones, Macronutrients, and Infant Characteristics on Gastric Emptying and Breastfeeding Patterns of Term Fully Breastfed Infants

Human milk (HM) components influence infant feeding patterns and nutrient intake, yet it is unclear how they influence gastric emptying (GE), a key component of appetite regulation. This study analyzed GE of a single breastfeed, HM appetite hormones/macronutrients and demographics/anthropometrics/body composition of term fully breastfed infants (n = 41, 2 and/or 5 mo). Stomach volumes (SV) were calculated from pre-/post-feed ultrasound scans, then repeatedly until the next feed. Feed volume (FV) was measured by the test-weigh method. HM samples were analyzed for adiponectin, leptin, fat, lactose, total carbohydrate, lysozyme, and total/whey/casein protein. Linear regression/mixed effect models were used to determine associations between GE/feed variables and HM components/infant anthropometrics/adiposity. Higher FVs were associated with faster (−0.07 [−0.10, −0.03], p < 0.001) GE rate, higher post-feed SVs (0.82 [0.53, 1.12], p < 0.001), and longer GE times (0.24 [0.03, 0.46], p = 0.033). Higher whey protein concentration was associated with higher post-feed SVs (4.99 [0.84, 9.13], p = 0.023). Longer GE time was associated with higher adiponectin concentration (2.29 [0.92, 3.66], p = 0.002) and dose (0.02 [0.01, 0.03], p = 0.005), and lower casein:whey ratio (−65.89 [−107.13, −2.66], p = 0.003). FV and HM composition influence GE and breastfeeding patterns in term breastfed infants.
The making of modern cynicism

Once describing a life of exile, self-denial, physical rigor, and mastery of one's desires, cynicism now describes a life of political quietism, passivity, and moral indifference, representing not a weakening of ancient philosophic norms but rather their inversion. In "The Making of Modern Cynicism", David Mazella asks: how did ancient Cynic philosophy come to provide a name for its modern, unphilosophical counterpart, and what events caused such a dramatic reversal of cynicism's former meanings? He traces the concept of cynicism from its origins as a philosophical way of life in Greek antiquity through its successive transformations in the early modern and Enlightenment periods and into the nineteenth century when it took its distinctively modern, unreflective form as a variety of disenchantment, disbelief, or distrust. Sampling a wide variety of literary, philosophical, and historical writings, Mazella documents the transition of the cynic from an ascetic philosopher to any person whose "faded belief or curdled trust had left him unfit for attachments to others. " Even more important, Mazella questions why cynicism should provoke such hand-wringing from cultural critics when it has been a stable, recognized, even routine feature of modern politics for the better part of 150 years. Arguing that modern cynics inspire powerful reactions by envisaging a future without hope of meaningful change, he then suggests that we address popular cynicism in more effective, less moralistic ways. Rather than dismissing cynicism as an irrational attitude of distrust or fatalism, or chiding cynics for their persistent disbelief, Mazella contends that analyzing cynicism can reveal the unacknowledged limits of current political argument, a crucial first step toward developing the kinds of reasoned persuasion necessary for more meaningful and substantive forms of political change. Well-written and engaging, "The Making of Modern Cynicism" will appeal not only to readers in literary and cultural criticism but also to those interested in political theory and the history of philosophy.
Synaptic Connections of Calretinin-Immunoreactive Neurons in the Human Neocortex

Previous immunocytochemical studies in the cerebral cortex of various species have shown that the calcium-binding protein calretinin (CR) labels specific subpopulations of nonspiny nonpyramidal cells (interneurons). The present study attempts to characterize morphologically and chemically the microcircuitry of CR-immunoreactive (CR-ir) neurons in the human temporal neocortex. Postembedding immunocytochemistry for CR and GABA and combination immunocytochemistry for CR and nonphosphorylated neurofilament protein (NPNFP) or for CR and the calcium-binding proteins parvalbumin (PV) and calbindin (CB) showed CR multiterminal endings frequently innervating the distal apical dendrite or the cell body and proximal dendrites of NPNFP-ir or CB-ir pyramidal cells, respectively. Cell bodies of interneurons immunoreactive for CB or PV were innervated only occasionally by CR multiterminal endings, whereas certain GABA neurons were surrounded by them. Furthermore, CR-ir axon terminals formed either symmetrical (the majority) or asymmetrical synapses with a variety of postsynaptic elements. These results indicate that different subpopulations of CR interneurons exist that are specialized for selective innervation of somatic or dendritic regions of certain pyramidal and nonpyramidal neurons.
Prevalence of Human Parechovirus in The Netherlands in 2000 to 2007

ABSTRACT Infection with human parechovirus 3 (HPeV3) was described for the first time in Japan in 2004 and reportedly is more often associated with severe disease than infection with HPeV1 or HPeV2. In 2004, infections with HPeV3 were observed for the first time in The Netherlands. Genetic analysis showed several different lineages, suggesting endemic circulation. We analyzed 163 cell culture isolates from the same number of patients tested in routine virological laboratories as part of the national enterovirus surveillance program. Isolates were collected between 2000 and 2007 and could not be characterized by routine methods. In total, 155 isolates (95%) were found positive for HPeV by a reverse transcription-PCR assay targeting the 5′ untranslated region, explaining the majority of the diagnostic deficit in enterovirus surveillance for these years. Typing of the isolates by use of partial genome sequencing of the VP1/2A region revealed the presence of 55 HPeV1, 2 HPeV2, 89 HPeV3, 1 HPeV4, and 8 HPeV5 isolates. We compared isolation dates, age groups affected, and clinical pictures, which were reported as part of the routine surveillance. Clear differences in epidemiology were observed, with HPeV3 occurring at intervals of 2 years and in the spring-summer season, whereas HPeV1 was observed in small numbers throughout each year, with a low in the summer months. HPeV3 infection affected younger children than HPeV1 infection and was significantly more often associated with fever, meningitis, and viremia.
Simple and Rapid Proteomic Analysis by Protease-Immobilized Microreactors

Proteomics is the large-scale study of proteins, particularly their stuructures and functions. One of the most important point is to develop efficient and rapid approachehes to identify the target proteins. Peptide mass mapping and tandem mass spectrometry (MS/MS)-based peptide sequencing are key methods in current protein identification for proteomic studies. Proteins are usually digested into peptides that are subsequently analyzed by MS. Therefore, proteolysis by sequence-specific proteases is the key step for positive sequencing in proteomic analysis integrated with MS (Aebersold & Mann, 2003). The conventional method of in-solution digestion by proteases is a time-consuming procedure (overnight at 37 C). The substrate/protease ratio must be kept high (generally > 50) in order to prevent excessive sample contamination by the protease and its auto-digested products. But this leads to a relatively slow digestion. In addition, obtaining reliable peptide maps and meaningful sequence data by MS analysis requires not only the separation of the digested peptides but also strictly defined proteolysis conditions (Domon & Aebersold, 2006; Witze et al., 2007). In addition, the ionization efficiency of the digested fragments including a modified peptide such as a phosphopeptide is dependent on peptide-size or peptidesequence, which directly correlates with sequence coverage of the target protein by MS analysis. Furthermore, peptide recovery from in-solution digestion is highly dependent on the structural properties of the target proteins because proteins with rigid structures, e.g. by disulfide bonds tend to be resistant to complete digestion. In fact, the typical preparation of a sample for proteolysis includes denaturation, reduction of disulfide bonds, and alkylation procedures lead to a decrease the conformational stability. It is obvious that insufficient sequence coverage could compromise the accuracy of proteome characterization. Therefore, it is important to develop novel digestion methods to achieve a highly efficient proteolysis for MS-based peptide mapping (Park & Russell, 2000; Slysz et al., 2006). A microreactor is a suitable reaction system for handling small-volume samples in a microchannel to perform chemical or enzymatic reactions. Enzyme-immobilized
Control of motor unit firing during step-like increases in voluntary force

In most skeletal muscles, force is generated by a combination of motor unit (MU) recruitment and increases in the firing rate of previously active MUs. Two contrasting patterns of firing rate organization have been reported. In the first pattern, the earliest recruited MUs reach the highest firing rates as force is increased, and later recruited MUs fire at lower rates. When firing rate of multiple MUs are superimposed, these rate trajectories form a concentric layered profile termed “onion skin. ” In the second pattern, called “reverse onion skin,” later recruited MUs reach higher firing rates, and crossing of firing rate trajectories for recorded MUs is common (although such trajectories are assembled routinely from different trials). Our present study examined the firing rate organization of concurrently active MUs of the first dorsal interosseous muscle during serial, step-like increases in isometric abduction forces. We used a surface sensor array coupled with MU discrimination algorithms to characterize MU firing patterns. Our objective was to determine whether “onion skin” profiles are contingent upon the force trajectory of the motor task, examined here using step-like increases of force output, and also whether they are manifested at different force levels. Our results revealed that the overall “onion skin” firing rate profile was retained as the force level increased with each force step up to 15% MVC. However, the distribution of firing rates across MUs was compressed with increasing force, and overlapping firing rate of units were observed. This rate compression was largely due to rate saturation of the relatively high frequency discharging MUs. Our results reflect flexible firing patterns across MUs at different levels of excitation drive. It is also evident that many units did not follow all the step increases consistently. This failure to track firing rate increases at higher forces could be due to an intrinsically mediated saturation of firing rates for the low threshold MUs, or potentially to some form of inhibitory interactions between active MUs as the level of excitation of the MU pool is progressively increased.
Health status of elderly persons of Antioquia, Colombia

Objective The health needs of older adults constitute challenges for health monitoring and care, resulting in increased costs. The present study therefore sought to analyze the health status of the elderly to enable targeting and intervention, in the pursuit of healthy aging for all. Method A quantitative observational cross-sectional study using a primary source was performed in 2012. A total of 4,248 adults aged over 60 years of age residing in the nine regions of Antioquia, Colombia, and the capital city were surveyed. Univariate, bivariate and multivariate analysis was performed of statistical and epidemiological measures, based on confidence intervals and statistical tests under 5%. Results One in four individuals had a risk of depression, one in three were at risk of anxiety, eight out of ten had cognitive impairment, mainly mild, and the same proportion were at nutritional risk. The elderly persons suffered from all types of abuse, notably psychological. Conclusions Thehealth status ofelderly personsis more affectedbymental than physical healthrisks, indicatingthatpublic policy anda better distribution ofresourcesshould be aimed at seeking animprovement in qualityof life anddignified aging, differentiated byregion.
Chimeric cytochromes P450 engineered by domain swapping and random mutagenesis for producing human metabolites of drugs

Human drug metabolites produced by cytochrome P450 enzymes are critical for safety testing and may themselves act as drugs or leads in the drug discovery and development process. Here, highly active chimeric fusion proteins (chimeras) were obtained by reductase domain swapping of mutants at key catalytic residues of the heme domain with that of a natural variant (CYP102A1.2) of P450 BM3 (CYP102A1.1) from Bacillus megaterium. Random mutagenesis at the heme domain of the chimera was also used to generate chimeric mutants that were more active and diverse than the chimeras themselves. To determine whether the chimeras and several mutants of the highly active chimera displayed enhanced catalytic activity and, more importantly, whether they acquired activities of biotechnological importance, we measured the oxidation activities of the chimeras and chimeric mutants toward human P450 substrates, mainly drugs. Some of the chimeric mutants showed high activity toward typical human P450 substrates including drugs. Statin leads, especially chiral products, with inhibitory effects toward HMG‐CoA reductase could be obtained from metabolites of statin drugs generated using these chimeric mutants. This study reveals the critical role of the reductase domain for the activity of P450 BM3 and shows that chimeras generated by domain swapping can be used to develop industrial enzymes for the synthesis of human metabolites from drugs and drug leads. Biotechnol. Bioeng. 2014;111: 1313–1322. © 2014 Wiley Periodicals, Inc.
Relationship of Ethnic Identity and Spiritual Development: An Exploratory Study

The current investigation examined the relationship of ethnic identity and spiritual development. The Multigroup Ethnic Identity Measure (J. Phinney, 1992), Spirituality Ends Measure (B. Fiorito & L. Ryan, 1998), and Spirituality Means Measure (B. Fiorito & L. Ryan, 1998) were administered to 198 male and female college students from 4 ethnic groups. Results revealed differences among ethnic groups in the way that they identified with their ethnic heritage and spiritual orientation. In addition, the results Indicated that ethnic identity was positively associated with an intrinsic spiritual means orientation and negatively related to an extrinsic spiritual ends orientation. Implications for counselors and recommendations for future research are discussed.
Young traffic victims' long-term health-related quality of life: child self-reports and parental reports.

OBJECTIVES To describe the long-term health-related quality of life (HRQOL) reported by young traffic injury victims and to assess the child-parent agreement on the child's HRQOL. DESIGN Cohort study with a mean follow-up of 2.4 years. SETTING Traumatology department in a university hospital in The Netherlands. PARTICIPANTS All traffic injury victims treated at the traumatology department in 1996-1997 and aged 8 to 15 years at follow-up (N = 254). The data of 157 child-parent pairs were available for analysis (mean follow-up age, 12+/-2.4 y; 57% boys; 24% hospitalized). INTERVENTIONS Not applicable. MAIN OUTCOME MEASURE TNO-AZL Children's Quality of Life parent and child questionnaires. RESULTS Young traffic injury victims reported a significantly lower HRQOL in the motor and autonomy scales compared with contemporaries in the reference group. The child-parent agreement ranged from low to moderate (intraclass correlations,.35-.67). A comparison made between the children and their parents found that the children were more negative regarding the physical complaints and the motor, autonomy, and positive-emotion scales. CONCLUSIONS Young traffic injury victims reported a reasonably good long-term HRQOL, and, surprisingly, few psychologic problems were revealed. Physicians who rely only on parental reports may overestimate the child's HRQOL, especially when assessing the physical functioning. The child's own reports should not be neglected in the assessment of a comprehensive picture of the child's HRQOL.
Combined Histopathological and Molecular Cytogenetic Stratification of Medulloblastoma Patients

This study examined the utility of stratifying children with medulloblastomas by a combination of refined histopathological classification and molecular cytogenetic evaluation. Detailed histopathological classification of tumors from a cohort of patients (n = 87) composed mainly of children entered into the International Society of Pediatric Oncology (SIOP)/United Kingdom Children’s Cancer Study Group PNET3 trial (n = 65), included identification of the large cell/anaplastic phenotype. Fluorescence in situ hybridization was used to detect chromosome 17 abnormalities, losses of 9q22 and 10q24, and amplification of the MYCC and MYCN oncogenes. The large cell/anaplastic phenotype, which was present in 20% of medulloblastomas, emerged as an independent prognostic indicator. Loss of 17p13.3 (38% of medulloblastomas) was found across all of the histopathological variants, whereas MYCC/MYCN amplification (6%/8% of medulloblastomas) was significantly associated with the large cell/anaplastic phenotype. Both of these genetic abnormalities emerged as prognostic indicators. Loss of 9q22 was associated with the nodular/desmoplastic medulloblastoma variant, whereas loss of 10q24 was found in all of the variants. Together with metastatic tumor at presentation, the large cell/anaplastic phenotype, 17p13.3 loss, or high-frequency MYC amplification defined a high-risk group of children whose outcome was significantly (P = 0.0002) poorer than a low-risk group without these tumor characteristics. Combined evaluation of novel histopathological features and molecular cytogenetic abnormalities promises to allow stratification of patients with medulloblastoma, such that those likely to be cured will be spared the side effects of maximal therapy, which can be targeted at those with aggressive disease.