Miracle-dz/UniFAR-FTUs huggingface dataset:
Photothrombosis ischemia stimulates a sustained astrocytic Ca2+ signaling in vivo

Although there is significant information concerning the consequences of cerebral ischemia on neuronal function, relatively little is known about functional responses of astrocytes, the predominant glial‐cell type in the central nervous system. In this study, we asked whether focal ischemia would impact astrocytic Ca2+ signaling, a characteristic form of excitability in this cell type. In vivo Ca2+ imaging of cortical astrocytes was performed using two‐photon (2‐P) microscopy during the acute phase of photothrombosis‐induced ischemia initiated by green light illumination of circulating Rose Bengal. Although whisker evoked potentials were reduced by over 90% within minutes of photothrombosis, astrocytes in the ischemic core remained structurally intact for a few hours. In vivo Ca2+ imaging showed that an increase in transient Ca2+ signals in astrocytes within 20 min of ischemia. These Ca2+ signals were synchronized and propagated as waves amongst the glial network. Pharmacological manipulations demonstrated that these Ca2+ signals were dependent on activation of metabotropic glutamate receptor 5 (mGluR5) and metabotropic γ‐aminobutyric acid receptor (GABABR) but not by P2 purinergic receptor or A1 adenosine receptor. Selective inhibition of Ca2+ in astrocytes with BAPTA significantly reduced the infarct volume, demonstrating that the enhanced astrocytic Ca2+ signal contributes to neuronal damage presumably through Ca2+‐dependent release of glial glutamate. Because astrocytes offer multiple functions in close communication with neurons and vasculature, the ischemia‐induced increase in astrocytic Ca2+ signaling may represent an initial attempt for these cells to communicate with neurons or provide feed back regulation to the vasculature. © 2008 Wiley‐Liss, Inc.
Issues in Empirical Studies with Students

Empirical studies in software engineering are often used to gain insight into new techniques and methods. However, due mainly to concerns of external validity, questions have been raised as to the value of these types of studies. In this paper, we discuss lessons learned from conducting a large number of empirical studies in university courses in three different countries (Italy, Norway and the United States). We provide a review of the relevant literature from the empirical software engineering community, the software engineering education community and the general education community. After identifying four important stakeholders in these studies (Researchers, Students, Instructors, and Professionals), we list the main goals and risks faced by each. We then use this information to develop a checklist that can be followed by researchers and educators when planning and running studies in university courses. The goal of this checklist is to provide guidance to ensure that such studies have as much experimental and pedagogical value as possible.
Ontogenic Expression Profiles of Gonadotropins (fshb and lhb) and Growth Hormone (gh) During Sexual Differentiation and Puberty Onset in Female Zebrafish1

ABSTRACT In the zebrafish model, the ontogenic expression profiles of all pituitary hormones have been reported except gonadotropins, partly because they are not supposed to be expressed in the embryonic stage. The spatiotemporal expression patterns of gonadotropins, namely follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh), in this species therefore remain largely unknown. As the master hormones controlling reproduction, the information on this issue would be valuable for understanding the roles of gonadotropins in early sexual development. Using double-colored fluorescent in situ hybridization (FISH) and real-time quantitative PCR (qPCR), this study was undertaken to analyze the ontogenic expression patterns of FSHbeta (fshb) and LHbeta (lhb) subunits in the zebrafish pituitary, with particular emphasis on the stage of sexual differentiation (∼25–30 dpf [days postfertilization]) and puberty onset (∼45 dpf). As a control, growth hormone (gh) was also examined throughout the study. The zebrafish were collected at different time points of early development, including 4, 5, 6, 8, 10, 13, 16, 19, 22, 25, 28, 38, 48, and 53 dpf. The head of each fish, including the brain and pituitary, was sampled for double-colored FISH analysis, whereas the body was fixed for histological examination of sex and gonadal developmental stage. Our results showed that the expression of fshb started much earlier than that of lhb, with its mRNA signal detectable (∼2–3 cells per pituitary) shortly after hatching (4 dpf). In contrast, lhb expression became detectable much later, at the time of sex differentiation (∼25 dpf). In female zebrafish, the first morphological sign for puberty is the first wave of follicle transition from the primary growth to previtellogenic stage, which occurs around 45 dpf and is marked by the appearance of cortical alveoli in the oocytes. Interestingly, the number of lhb-expressing cells was very low (∼5–6 cells per pituitary) before this transition but increased dramatically during and after the transition. In contrast, the expression of fshb was abundant before puberty, with only a slight increase in cell number during puberty onset. The increased expression of fshb and lhb at puberty was also supported by real-time qPCR analysis at the single pituitary level. Interestingly, the fshb-expressing cells changed their spatial distribution significantly during puberty, from a predominantly peripheral to a central location. As the control, the expression of gh was abundant throughout prepubertal and pubertal periods. Our results strongly suggest an important role for Lh at the puberty onset of female zebrafish, similar to the situation in mammals, and its expression could be a sign for puberty at the pituitary level. However, the significance of the location change of Fsh cells during this period will be interesting to investigate.
USING MENDELIAN RANDOMISATION TO INFER CAUSALITY IN DEPRESSION AND ANXIETY RESEARCH

Depression and anxiety co‐occur with substance use and abuse at a high rate. Ascertaining whether substance use plays a causal role in depression and anxiety is difficult or impossible with conventional observational epidemiology. Mendelian randomisation uses genetic variants as a proxy for environmental exposures, such as substance use, which can address problems of reverse causation and residual confounding, providing stronger evidence about causality. Genetic variants can be used instead of directly measuring exposure levels, in order to gain an unbiased estimate of the effect of various exposures on depression and anxiety. The suitability of the genetic variant as a proxy can be ascertained by confirming that there is no relationship between variant and outcome in those who do not use the substance. At present, there are suitable instruments for tobacco use, so we use that as a case study. Proof‐of‐principle Mendelian randomisation studies using these variants have found evidence for a causal effect of smoking on body mass index. Two studies have investigated tobacco and depression using this method, but neither found strong evidence that smoking causes depression or anxiety; evidence is more consistent with a self‐medication hypothesis. Mendelian randomisation represents a technique that can aid understanding of exposures that may or may not be causally related to depression and anxiety. As more suitable instruments emerge (including the use of allelic risk scores rather than individual single nucleotide polymorphisms), the effect of other substances can be investigated. Linkage disequilibrium, pleiotropy, and population stratification, which can distort Mendelian randomisation studies, are also discussed.
Temporal Self-Extension: Implications for Temporal Comparison and Autobiographical Memory

people should also chronically differ in how far their current self subjectively reaches into the past. The model of personal memory (Ross, Abstract : Research on temporal comparison has shown that people dissociate themselves from their past to attain a positive self view. Social comparison research has demonstrated that the distinctness of contextually activated information determines whether a recalled self exerts assimilation or contrast effects on the current self. However, hardly any study addressed individual differences. Also, very little is known about whether the ease or difficulty to date past events and experiences influences current self-judgments. We present a new scale capturing the degree of the current self time extension. Three studies support the notion that temporal self-extension determines how past selves are accessed and processed, regarding both the abstractness with which self-knowledge is retrieved and the experienced temporal distance to the past. These findings have important implications for temporal as well as social comparison processes.
Analysis of quantization noise feedback in causal transform coding

The performances of the LDU (lower-diagonal-upper) factorization transform were recently shown to be equivalent to those of the Karhunen-Loeve transform (KLT), which is optimal for Gaussian sources, in the limit of high rates (Phoong, S.-M. and Lin, Y.-P., 2000; Mary, D. and Slock, D.T.M., 2001; Lahouti, F. and Khandani, A.K., 2001). We further investigate the performances of the LDU for actual transform coding (TC) schemes. Our previous results (Mary and Slock, 2001) showed that the LDU should be implemented in closed loop around the quantizers, though this leads to a noise feedback effect, similar to that occurring in DPCM systems. We develop novel analyses of these effects on the distortion-rate functions and coding gains. The proposed analyses compare our previous results, obtained for a hypothetical TC system for which the bit allocation is optimal and the rate is high, to those obtained for practical TC systems whose bit allocation is nearly optimal. By means of a theorem and numerical results, evidence is given that ordering the subsignals in the source vector by order of decreasing variance minimizes the quantization noise feedback. For the investigated practical systems, we show that deviations from the high rate assumptions arise below /spl sim/3 b/s. The effects of the noise feedback become non negligible below /spl sim/2 b/s. The LDU competes with the KLT above /spl sim/2.5 b/s.
TO AGREE OR NOT TO AGREE: THE EFFECTS OF VALUE CONGRUENCE, INDIVIDUAL DEMOGRAPHIC DISSIMILARITY, AND CONFLICT ON WORKGROUP OUTCOMES

In this quasi‐experimental study we investigate value congruence and demographic dissimilarity among group members as factors which influence various types of conflict within workgroups. We also examine whether it is beneficial for members to be different or alike, to agree or disagree, in order to foster work group productivity. Results indicate that visible individual demographic differences (i.e., sex, age) increase relationship conflict, while informational demographic differences (i.e., education) increase task‐focused conflict. Value congruence of members decreased both relationship and task conflict, and the specific content of the values held by members influenced performance. Specifically, both detail and outcome group value orientations increased objective performance; outcome, decisiveness, and stability orientations increased perceptions of high performance; and both decisiveness and supportiveness orientations increased the satisfaction level of group members while a team orientation decreased individual member satisfaction in this sample.
Using Commitments to Drive Consistency: Enhancing the Effectiveness of Cause‐related Marketing Communications

Research has consistently found that, despite a high degree of expressed concern about the environment, few individuals are willing to follow‐up this concern with behavioural actions in support of the environment, particularly when these actions require the individuals to absorb some costs. Using the theoretical framework of commitment–consistency theory, two studies were conducted in order to examine the effect of a small, active commitment to an environmental cause on consumer preference for an advertised product that supported that cause. The empirical results show strong support for the fact that persuading consumers to make an active commitment to a cause can serve as an important prerequisite for behavioural consistency (in terms of the willingness to buy a product). Further, the paper adds to the evidence on self‐perception theory as an underlying mechanism for the effectiveness of the technique. It is also found that, although commitment increases willingness to buy a product related to the cause, the effect works only when the cost associated with supporting the cause is minimal. The implications for marketing communications theory and practice are discussed.
Fast Concept Analysis

Formal concept analysis is increasingly used for large contexts that are built by programs. This paper presents an efficient algorithm for concept analysis that computes concepts together with their explicit lattice structure. An experimental evaluation uses randomly generated contexts to compare the running time of the presented algorithm with two other algorithms. Running time increases quadratically with the number of concepts, but with a small quadratic component. At least contexts with sparsely filled context tables cause concept lattices grow quadratically with respect to the size of their base relation. The growth rate is controlled by the density of context tables. Modest growth combined with efficient algorithms lead to fast concept analysis.
Implementation of Motor Imagery during Specific Aerobic Training Session in Young Tennis Players

The aim of this study was to investigate the effects of implementing motor imagery (MI) during specific tennis high intensity intermittent training (HIIT) sessions on groundstroke performance in young elite tennis players. Stroke accuracy and ball velocity of forehand and backhand drives were evaluated in ten young tennis players, immediately before and after having randomly performed two HIIT sessions. One session included MI exercises during the recovery phases, while the other included verbal encouragements for physical efforts and served as control condition. Results revealed that similar cardiac demand was observed during both sessions, while implementing MI maintained groundstroke accuracy. Embedding MI during HIIT enabled the development of physical fitness and the preservation of stroke performance. These findings bring new insight to tennis and conditioning coaches in order to fulfil the benefits of specific playing HIIT sessions, and therefore to optimise the training time.
Call Center Outsourcing Contract Analysis and Choice

This paper considers a call center outsourcing contract analysis and choice problem faced by a contractor and a service provider. The service provider receives an uncertain call volume over multiple periods and is considering outsourcing all or part of these calls to a contractor. Each call brings in a fixed revenue to the service provider. Answering calls requires having service capacity; thus implicit in the outsourcing decision is a capacity decision. Insufficient capacity implies that calls cannot be answered, which in turn means there will be a revenue loss. Faced with a choice between a volume-based and a capacity-based contract offered by a contractor that has pricing power, the service provider determines optimal capacity levels. The optimal price and capacity of the contractor together with the optimal capacity of the service provider determine optimal profits of each party under the two contracts being considered. This paper characterizes optimal capacity levels and partially characterizes optimal pricing decisions under each contract. The impact of demand variability and the economic parameters on contract choice are explored through numerical examples. It is shown that no contract type is universally preferred and that operating environments as well as cost-revenue structures have an important effect.
Interacting oscillatory boundary layers and wall modes in modulated rotating convection

Thermal convection in a rotating cylinder near onset is investigated using direct numerical simulations of the Navier–Stokes equations with the Boussinesq approximation in a regime dominated by the Coriolis force. For thermal driving too small to support convection throughout the entire cell, convection sets in as alternating pairs of hot and cold plumes in the sidewall boundary layer, the so-called wall modes of rotating convection. We subject the wall modes to small amplitude harmonic modulations of the rotation rate over a wide range of frequencies. The modulations produce harmonic Ekman boundary layers at the top and bottom lids as well as a Stokes boundary layer at the sidewall. These boundary layers drive a time-periodic large-scale circulation that interacts with the wall-localized thermal plumes in a non-trivial manner. The resultant phenomena include a substantial shift in the onset of wall-mode convection to higher temperature differences for a broad band of frequencies, as well as a significant alteration of the precession rate of the wall mode at very high modulation frequencies due to the mean azimuthal streaming flow resulting from the modulations.
Minecraft and the Building Blocks of Creative Individuality

This article situates the video game Minecraft along with Robinson Crusoe in a genealogy of island fictions to show how Minecraft reinterprets narratives of creative subjects and invention. By reading Minecraft through histories of construction toys, modern conceptions of creativity, and digital practices of modding, I argue that Minecraft allows players to experiment with various social and environmental conditions historically central to debates over shaping creative subjects, rooted in island narratives as the generic site for negotiating conflicts between individual autonomy and social dependence. I show how Minecraft players become inventive subjects through simulating Crusoe’s island and performing code work with the game as software embedded in networks of players, programmers, and digital technologies.
THE FINE STRUCTURE OF Streptomyces coelicolor II. The Nuclear Material

Colonies and spore suspensions of Streptomyces coelicolor were fixed for electron microscopy by the method of Kellenberger, Ryter, and Sechaud (1958). In thin sections the nuclear regions have a lower average density than the cytoplasm and the outlines of these regions correspond well with the profiles of the chromatinic bodies observed with the light microscope. The nuclear regions contain fibrils, about 5 mµ in diameter. In contrast, after fixation by the method of Palade (1952) the nuclear material is coagulated into irregular dense masses and tubular structures about 20 mµ in diameter, lying in a nuclear "vacuole. " The significance of these observations is discussed in relation to the observations of other workers on the fine structure of the nuclear material of other bacteria and the chromosomes of higher cells.
Homogeneous functions on light cones: the infinitesimal structure of some degenerate principal series representations

In this paper we study the reducibility, composition series and unitarity of the components of some degenerate principal series representations of $\RMO(p,q)$, $\RMU(p,q)$ and $\SP(p,q)$. This is done by realizing these representations in paces of homogeneous functions on light cones and writing down the explicit actions of the universal enveloping algebra of the group concerned.
Xanthine Oxidase Activity Is Associated with Risk Factors for Cardiovascular Disease and Inflammatory and Oxidative Status Markers in Metabolic Syndrome: Effects of a Single Exercise Session

Objective. The main goal of the present study was to investigate the xanthine oxidase (XO) activity in metabolic syndrome in subjects submitted to a single exercise session. We also investigated parameters of oxidative and inflammatory status. Materials/Methods. A case-control study (9 healthy and 8 MS volunteers) was performed to measure XO, superoxide dismutase (SOD), glutathione peroxidase activities, lipid peroxidation, high-sensitivity C-reactive protein (hsCRP) content, glucose levels, and lipid profile. Body mass indices, abdominal circumference, systolic and diastolic blood pressure, and TG levels were also determined. The exercise session consisted of 3 minutes of stretching, 3 minutes of warm-up, 30 minutes at a constant dynamic workload at a moderate intensity, and 3 minutes at a low speed. The blood samples were collected before and 15 minutes after the exercise session. Results. Serum XO activity was higher in MS group compared to control group. SOD activity was lower in MS subjects. XO activity was correlated with SOD, abdominal circumference, body mass indices, and hsCRP. The single exercise session reduced the SOD activity in the control group. Conclusions. Our data support the association between oxidative stress and risk factors for cardiovascular diseases and suggest XO is present in the pathogenesis of metabolic syndrome.
A DESIGN OF EXPERIMENTS APPROACH TO READINESS RISK ANALYSIS

We develop a simulation model to aid in identifying and evaluating promising alternatives to achieve improvements in weapon system-level availability when services for system components are outsourced. Two outcomes are valued: improvements in average operational availability for the weapon system, and reductions in the probability that operational availability of the weapon system falls below a given planning threshold (readiness risk). In practice, these outcomes must be obtained through performance-based agreements with logistics providers. The size of the state space, and the non-linear and stochastic nature of the outcomes, precludes the use of optimization approaches. Instead, we use designed experiments to evaluate simulation scenarios in an intelligent way. This is an efficient approach that enables us to assess average readiness and readiness risk outcomes of the alternatives, as well as to identify the components and logistics factors with the greatest impact on operational availability.
Discrete Representation of Straight Lines

If a continuous straight line segment is digitized on a regular grid, obviously a loss of information occurs. As a result, the discrete representation obtained (e.g., a chaincode string) can be coded more conveniently than the continuous line segment, but measurements of properties (such as line length) performed on the representation have an intrinsic inaccuracy due to the digitization process. In this paper, two fundamental properties of the quantization of straight line segments are treated. 1) It is proved that every ``straight'' chaincode string can be represented by a set of four unique integer parameters. Definitions of these parameters are given. 2) A mathematical expression is derived for the set of all continuous line segments which could have generated a given chaincode string. The relation with the chord property is briefly discussed.