Miracle-dz/UniFAR-FTUs huggingface dataset:
Exposure to Endocrine Disruptor Induces Transgenerational Epigenetic Deregulation of MicroRNAs in Primordial Germ Cells

In mammals, germ cell differentiation is initiated in the Primordial Germ Cells (PGCs) during fetal development. Prenatal exposure to environmental toxicants such as endocrine disruptors may alter PGC differentiation, development of the male germline and induce transgenerational epigenetic disorders. The anti-androgenic compound vinclozolin represents a paradigmatic example of molecule causing transgenerational effects on germ cells. We performed prenatal exposure to vinclozolin in mice and analyzed the phenotypic and molecular changes in three successive generations. A reduction in the number of embryonic PGCs and increased rate of apoptotic cells along with decrease of fertility rate in adult males were observed in F1 to F3 generations. Blimp1 is a crucial regulator of PGC differentiation. We show that prenatal exposure to vinclozolin deregulates specific microRNAs in PGCs, such as miR-23b and miR-21, inducing disequilibrium in the Lin28/let-7/Blimp1 pathway in three successive generations of males. As determined by global maps of cytosine methylation, we found no evidence for prominent changes in DNA methylation in PGCs or mature sperm. Our data suggest that embryonic exposure to environmental endocrine disruptors induces transgenerational epigenetic deregulation of expression of microRNAs affecting key regulatory pathways of germ cells differentiation.
Eclipse-induced wind changes over the British Isles on the 20 March 2015

The British Isles benefits from dense meteorological observation networks, enabling insights into the still-unresolved effects of solar eclipse events on the near-surface wind field. The near-surface effects of the solar eclipse of 20 March 2015 are derived through comparison of output from the Met Office’s operational weather forecast model (which is ignorant of the eclipse) with data from two meteorological networks: the Met Office’s land surface station (MIDAS) network and a roadside measurement network operated by Vaisala. Synoptic-evolution relative calculations reveal the cooling and increase in relative humidity almost universally attributed to eclipse events. In addition, a slackening of wind speeds by up to about 2 knots in already weak winds and backing in wind direction of about 20° under clear skies across middle England are attributed to the eclipse event. The slackening of wind speed is consistent with the previously reported boundary layer stabilization during eclipse events. Wind direction changes have previously been attributed to a large-scale ‘eclipse-induced cold-cored cyclone’, mountain slope flows, and changes in the strength of sea breezes. A new explanation is proposed here by analogy with nocturnal wind changes at sunset and shown to predict direction changes consistent with those observed. This article is part of the themed issue ‘Atmospheric effects of solar eclipses stimulated by the 2015 UK eclipse’.
Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene

In this communication, we describe several features of the D. melanogaster gene which codes for ribosomal protein 49 (rp49). Nucleotide sequence analysis in conjunction with primer extension and S1 nuclease protection experiments show that the structure of the rp49 gene consists of a 102 bp 5' exon, a single 59 bp intron, and a 420 bp 3' exon, encoding a total of 132 amino acids. The rp49 gene shares many features with other abundantly expressed Drosophila genes, including codon preference, which are discussed.
Screening for lung cancer.

BACKGROUND While population based screening for lung cancer has not been adopted by most countries, it is not clear whether sputum examinations, chest radiography or newer methods such as computed tomography are effective in reducing mortality from lung cancer. OBJECTIVES To determine whether screening for lung cancer using regular sputum examinations or chest radiography or CT chest reduces lung cancer mortality. SEARCH STRATEGY Electronic databases (the Cochrane Central Register of Controlled Trials, MEDLINE, PREMEDLINE and EMBASE; 1966 to July 2000) ), bibliographies, hand searching of a journal and discussion with experts were used to identify published and unpublished trials. SELECTION CRITERIA Controlled trials of screening for lung cancer using sputum examinations, chest radiography or CT chest. DATA COLLECTION AND ANALYSIS Intention to screen analysis was performed. Where there was significant statistical heterogeneity relative risks were reported using the random effects model, but for other outcomes the fixed effect model was used. MAIN RESULTS Seven trials were included (6 randomised controlled studies and 1 non-randomised controlled trial) with a total of 245,610 subjects. There were no studies with an unscreened control group. Frequent screening with chest x-rays was associated with an 11% relative increase in mortality from lung cancer compared with less frequent screening (RR 1.11, CI: 1.00-1.23). A non statistically significant trend was observed to reduced mortality from lung cancer when screening with chest x-ray and sputum cytology was compared with chest x-ray alone (RR 0.88, CI:0.74-1.03). Several of the included studies had potential methodological weaknesses. There were no controlled studies of spiral CT. REVIEWER'S CONCLUSIONS The current evidence does not support screening for lung cancer with chest radiography or sputum cytology. Frequent chest x-ray screening might be harmful. Further, methodologically rigorous trials are required.
Entering the World of Individual Routines: The Affordances of Mobile Applications

The IS discipline has a long tradition in investigating how new technologies affect work practices, but has mostly focused on the organizational level. With mobile applications, we are facing a new technology wave that is centered on the individual users. Despite their popularity, mobile applications' possibilities to enhance an individual’s knowledge, skills, and competence in daily work practices have not been studied in a systematic way. Building on the concept of routines from organizational theory and insights from two field studies, we investigate mobile applications acting as material artifacts and their possibilities of goal-oriented actions in individual routines. Our main contributions are the extension of Pentland & Feldman’s generative system model and a set of affordances that mobile applications bring to individual routines. Our findings complement recent studies on routines at the organizational level and contribute to enhance artifact design knowledge for mobile applications beyond “interaction design”.
HISTOPATHOLOGICAL STUDY OF PROSTATIC LESIONS AND ASSESSEMENT WITH AGNOR INDEX

Interpretation of prostatic biopsies has been a continuous problem for practicing pathologist. This current study aims at the analysis of histopathological features of various non neoplastic and neoplastic lesions of the prostate including the grading of malignant lesions and evaluation of role of AgNOR index in different benign, premalignant and malignant lesions of prostate. One hundred and eight transurethral resection of prostate specimens were studied with haematoxylin and eosin and AgNOR staining. Benign prostatic hyperplasia was the most common lesion affecting the prostate in elderly. High grade prostatic intra epithelial neoplasia had strong association with prostatic carcinoma. Among the malignant lesions of the prostate, primary prostatic adenocarcinoma was the commonest. According to Gleason Grading system higher grades were more commonly observed as the predominant pattern. Mean AgNOR counts were higher in malignant lesions when compared with the benign lesions.
Use of MicroRNAs to Screen for Colon Cancer

Colon cancer (CC) screening is important for diagnosing early stage for malignancy and therefore potentially reduces mortality from this disease because the cancer could be cured at the early disease stage. Early detection is needed if accurate and cost effective diagnostic methods are available. Mortality from colon cancer malignancy is theoretically preventable through screening. The Current screening method, the immunological fecal occult blood test, FOBTi, lacks sensitivity and requires dietary restriction, which impedes compliance. Moreover colonoscopy is invasive and costly, which decreases compliance, and in certain cases could lead to mortality. Compared to the FOBT test, a noninvasive sensitive screen that does not require dietary restriction would be more convenient. Colonoscopy screening is recommended for colorectal cancer (CRC). Although it is a reliable screening method, colonoscopy is an invasive test, often accompanied by abdominal pain, has potential complications and has high cost, which has hampered its application worldwide. A screening approach that uses the relatively stable and non degradable micro RNA molecules when extracted from either the noninvasive human stool, or the semi-invasive blood samples by available commercial kits and manipulated thereafter, would be more preferable than a transcriptomic messenger (m) RNA-, a mutation DNA-, an epigeneticor a proteomic-based test. That approach utilizes reverse transcriptase (RT), followed by a modified quantitative real-time polymerase chain reaction (qPCR). To compensate for exosomal miRNAs that would not be measured, a parallel test could be performed on stool or plasma's total RNAs, and corrections for exsosomal loss are made to obtain accurate results. Ultimately, a chip would be developed to facilitate diagnosis, as has been carried out for the quantification of genetically modified organisms (GMOs) in foods. The gold standard to which the miRNA test is compared to is colonoscopy. If laboratory performance criteria are met, a miRNA test in human stool or blood samples based on high through put automated technologies and quantitative expression measurements currently employed in the diagnostic clinical laboratory, would eventually be advanced to the clinical setting, making a noticeable impact on the prevention of colon cancer.
It’s no Riddle, Choose the Middle

This study examines the effect of the number of crimes and topographical detail on police officer predictions of serial burglars’ home locations. Officers are given 36 maps depicting three, five, or seven crime sites and topographical or no topographical details. They are asked to predict, by marking an X on the map, where they thought each burglar lived. After making their predictions on half of the maps, officers randomly receive either no training or training in one of two simple decision-making strategies. The accuracy of predictions at baseline and retest is measured as the distance between the predicted and actual home locations, and these accuracy scores are compared to a commonly used geographic profiling system. Results show that training significantly improved predictive accuracy, regardless of the number of crime locations or topographical detail presented. In addition, trained participants are as accurate as the geographic profiling system.
Perception of the Combined Effects of Smoking and Alcohol on Cancer Risks in Never Smokers and Heavy Smokers

The issue addressed in the study is: How do people perceive the combined effect of alcohol consumption and tobacco consumption on risks of cancer? The method used was an application of Information Integration Theory. Sixty-four participants of both sexes were asked to estimate the risk of cancer associated with a number of situations described by a tobacco-consumption level associated with an alcohol-consumption level. Participants were subsequently presented with a questionnaire concerning the way alcohol and tobacco consumption can cause cancer. Results showed that French adults apparently considered that indulging in only one of these two behaviours represents a maximum health risk. The two effects were seen to combine disjunctively which runs counter to current medical data. However, there was total contradiction between the participants’ answers to the questionnaire concerning their knowledge and the information integration task.
Free and forced morphodynamics of river bifurcations

Water and sediment distribution by river bifurcations is often highly unbalanced. This may result from a variety of factors, such as migration of bars, channel curvature and backwater effects, which promote an uneven partition of flow and sediment fluxes in the downstream branches, which we call ‘forcings’. Bifurcations also display an intrinsic instability mechanism that leads to unbalanced configurations, as occurs in the idealized case of a geometrically symmetric bifurcation, which we call ‘free’, provided the width‐to‐depth ratio of the incoming flow is large enough. Most frequently, these free and forced mechanisms coexist; however, their controlling roles in bifurcation dynamics have not been investigated so far. In this paper we address this question by proposing a unified free‐forced modelling framework for bifurcation morphodynamics. Upstream channel curvature and different slopes of downstream branches (slope advantage) are specifically investigated as forcing effects typically occurring in bifurcations of alluvial channels. The modelling strategy is based on the widely used two‐cell model of Bolla Pittaluga et al. (Water Resources Research, 2003, 39(3), 1–13), here extended to account for the spatially non‐uniform fluxes entering the bifurcation node. Results reveal that the relative role of free and forced mechanisms depends on the width‐to‐depth ratio falling above or below the resonant threshold that controls the stability of free bifurcations: when the main channel is relatively wide and shallow (super‐resonant regime) the bifurcation invariably evolves towards unbalanced configurations, whatever the combination of curvature and slope advantage values, which instead control the bifurcation response under sub‐resonant conditions. Detection of the resonant aspect ratio as a key threshold also releases the modelling approach from the need for parameter calibration that characterized previous approaches, and allows for interpreting under a unified framework the opposite behaviours shown by gravel‐bed and sand‐bed bifurcations for increasing Shields parameter values. © 2018 John Wiley & Sons, Ltd.
Removing photography artifacts using gradient projection and flash-exposure sampling

Flash images are known to suffer from several problems: saturation of nearby objects, poor illumination of distant objects, reflections of objects strongly lit by the flash and strong highlights due to the reflection of flash itself by glossy surfaces. We propose to use a flash and no-flash (ambient) image pair to produce better flash images. We present a novel gradient projection scheme based on a gradient coherence model that allows removal of reflections and highlights from flash images. We also present a brightness-ratio based algorithm that allows us to compensate for the falloff in the flash image brightness due to depth. In several practical scenarios, the quality of flash/no-flash images may be limited in terms of dynamic range. In such cases, we advocate using several images taken under different flash intensities and exposures. We analyze the flash intensity-exposure space and propose a method for adaptively sampling this space so as to minimize the number of captured images for any given scene. We present several experimental results that demonstrate the ability of our algorithms to produce improved flash images.
Esthesioneuroblastoma: An Update on the Massachusetts Eye and Ear Infirmary and Massachusetts General Hospital Experience with Craniofacial Resection, Proton Beam Radiation, and Chemotherapy

Abstract Objectives To update the Massachusetts General Hospital (MGH) and Massachusetts Eye and Ear Infirmary (MEEI) experience in the management of esthesioneuroblastoma (ENB) with multimodality therapy and to reassess treatment outcomes and complications in a larger cohort with longer follow-up times. Design A retrospective chart review. Setting A tertiary referral center. Participants All patients presenting with ENB and managed at the MGH and MEEI from 1997 to 2013. Main Outcome Measures Disease-free and overall survival. Results Twenty-two patients were identified with an average follow-up of 73 months. Ten patients presented with Kadish stage B disease and 12 with stage C disease. A total of six patients (27%) developed regional metastases. Treatment for all patients included craniofacial resection (CFR) followed by proton beam irradiation with or without chemotherapy. The 5-year disease-free and overall survival rates were 86.4% and 95.2%, respectively, by Kaplan-Meier analysis. Negative margins were a significant factor in disease-free survival. One patient experienced severe late-radiation toxicity. Conclusions ENB is safely and effectively treated with CFR followed by proton beam irradiation. The high incidence of regional metastases warrants strong consideration for elective neck irradiation. Proton beam radiation is associated with lower rates of severe late-radiation toxicity than conventional radiotherapy.
Collaborative Networks as Determinants of Knowledge Diffusion Patterns

This paper examines whether interpersonal networks help explain two widely documented patterns of knowledge diffusion: (1) geographic localization of knowledge flows, and (2) concentration of knowledge flows within firm boundaries. I measure knowledge flows using patent citation data, and employ a novel regression framework based on choice-based sampling to estimate the probability of knowledge flow between inventors of any two patents. As expected, intraregional and intrafirm knowledge flows are found to be stronger than those across regional or firm boundaries. I explore whether these patterns can be explained by direct and indirect network ties among inventors, as inferred from past collaborations among them. The existence of a tie is found to be associated with a greater probability of knowledge flow, with the probability decreasing as the path length (geodesic) increases. Furthermore, the effect of regional or firm boundaries on knowledge flow decreases once interpersonal ties have been accounted for. In fact, being in the same region or firm is found to have little additional effect on the probability of knowledge flow among inventors who already have close network ties. The overall evidence is consistent with a view that interpersonal networks are important in determining observed patterns of knowledge diffusion.
Generic Trace Semantics via Coinduction

Trace semantics has been defined for various kinds of state-based systems, notably with different forms of branching such as non-determinism vs. probability. In this paper we claim to identify one underlying mathematical structure behind these "trace semantics," namely coinduction in a Kleisli category. This claim is based on our technical result that, under a suitably order-enriched setting, a final coalgebra in a Kleisli category is given by an initial algebra in the category Sets. Formerly the theory of coalgebras has been employed mostly in Sets where coinduction yields a finer process semantics of bisimilarity. Therefore this paper extends the application field of coalgebras, providing a new instance of the principle "process semantics via coinduction. "
Education of staff in preschool aged classrooms in child care centers and child outcomes: A meta-analysis and systematic review

Staff education is considered key to quality of early childhood education and care (ECEC) programs. However, findings about associations between staff education and children’s outcomes have been inconsistent. We conducted a systematic review and meta-analysis of associations between ECEC staff education and child outcomes. Searches of Medline, PsycINFO, and ERIC, websites of large datasets and reference sections of all retrieved articles were conducted. Eligible studies provided a statistical link between staff education and child outcomes for preschool-aged children in ECEC programs. Titles, abstracts and paper reviews as well as all data extraction were conducted by two independent raters. Of the 823 studies reviewed for eligibility, 39 met our inclusion criteria. Research in this area is observational in nature and subject to the inherent biases of that research design. Results from our systematic review were hampered by heterogeneity in how staff education was defined, variability in whose education was measured and the child outcomes that were assessed. However, overall the qualitative summary indicates that associations between staff education and childhood outcomes are non-existent to very borderline positive. In our meta-analysis of more homogeneous studies we identified certain positive, albeit very weak, associations between staff education and children’s language outcomes (specifically, vocabulary and letter word identification) and no significant association with a mathematics outcome (WJ Applied Problems). Thus, our findings suggest that within the range of education levels found in the existing literature, education is not a key driver of child outcomes. However, since we only explored levels of education that were reported in the literature, our findings cannot be used to argue for lowering education standards in ECEC settings.
Enhancing Diagnostic Precision: Evaluation of Preprocessing Filters in Simple Diffusion Kurtosis Imaging for Head and Neck Tumors

Background: Our initial clinical study using simple diffusion kurtosis imaging (SDI), which simultaneously produces a diffusion kurtosis image (DKI) and an apparent diffusion coefficient map, confirmed the usefulness of SDI for tumor diagnosis. However, the obtained DKI had noticeable variability in the mean kurtosis (MK) values, which is inherent to SDI. We aimed to improve this variability in SDI by preprocessing with three different filters (Gaussian [G], median [M], and nonlocal mean) of the diffusion-weighted images used for SDI. Methods: The usefulness of filter parameters for diagnosis was examined in basic and clinical studies involving 13 patients with head and neck tumors. Results: The filter parameters, which did not change the median MK value, but reduced the variability and significantly homogenized the MK values in tumor and normal tissues in both basic and clinical studies, were identified. In the receiver operating characteristic curve analysis for distinguishing tumors from normal tissues using MK values, the area under curve values significantly improved from 0.627 without filters to 0.641 with G (σ = 0.5) and 0.638 with M (radius = 0.5). Conclusions: Thus, image pretreatment with G and M for SDI was shown to be useful for improving tumor diagnosis in clinical practice.