Miracle-dz/UniFAR-FTUs huggingface dataset:
Estrogen Stimulates Proliferation and Differentiation of Neural Stem/Progenitor Cells through Different Signal Transduction Pathways

Our previous study indicated that both 17β-estradiol (E2), known to be an endogenous estrogen, and bisphenol A (BPA), known to be a xenoestrogen, could positively influence the proliferation or differentiation of neural stem/progenitor cells (NS/PCs). The aim of the present study was to identify the signal transduction pathways for estrogenic activities promoting proliferation and differentiation of NS/PCs via well known nuclear estrogen receptors (ERs) or putative membrane-associated ERs. NS/PCs were cultured from the telencephalon of 15-day-old rat embryos. In order to confirm the involvement of nuclear ERs for estrogenic activities, their specific antagonist, ICI-182,780, was used. The presence of putative membrane-associated ER was functionally examined as to whether E2 can activate rapid intracellular signaling mechanism. In order to confirm the involvement of membrane-associated ERs for estrogenic activities, a cell-impermeable E2, bovine serum albumin-conjugated E2 (E2-BSA) was used. We showed that E2 could rapidly activate extracellular signal-regulated kinases 1/2 (ERK 1/2), which was not inhibited by ICI-182,780. ICI-182,780 abrogated the stimulatory effect of these estrogens (E2 and BPA) on the proliferation of NS/PCs, but not their effect on the differentiation of the NS/PCs into oligodendroglia. Furthermore, E2-BSA mimicked the activity of differentiation from NS/PCs into oligodendroglia, but not the activity of proliferation. Our study suggests that (1) the estrogen induced proliferation of NS/PCs is mediated via nuclear ERs; (2) the oligodendroglial generation from NS/PCs is likely to be stimulated via putative membrane-associated ERs.
Anthrax Edema Factor Toxicity Is Strongly Mediated by the N-end Rule

Anthrax edema factor (EF) is a calmodulin-dependent adenylate cyclase that converts adenosine triphosphate (ATP) into 3’–5’-cyclic adenosine monophosphate (cAMP), contributing to the establishment of Bacillus anthracis infections and the resulting pathophysiology. We show that EF adenylate cyclase toxin activity is strongly mediated by the N-end rule, and thus is dependent on the identity of the N-terminal amino acid. EF variants having different N-terminal residues varied by more than 100-fold in potency in cultured cells and mice. EF variants having unfavorable, destabilizing N-terminal residues showed much greater activity in cells when the E1 ubiquitin ligase was inactivated or when proteasome inhibitors were present. Taken together, these results show that EF is uniquely affected by ubiquitination and/or proteasomal degradation.
Experience Matters

Deciding which piece of information to acquire or attend to is fundamental to perception, categorization, medical diagnosis, and scientific inference. Four statistical theories of the value of information—information gain, Kullback-Liebler distance, probability gain (error minimization), and impact—are equally consistent with extant data on human information acquisition. Three experiments, designed via computer optimization to be maximally informative, tested which of these theories best describes human information search. Experiment 1, which used natural sampling and experience-based learning to convey environmental probabilities, found that probability gain explained subjects’ information search better than the other statistical theories or the probability-of-certainty heuristic. Experiments 1 and 2 found that subjects behaved differently when the standard method of verbally presented summary statistics (rather than experience-based learning) was used to convey environmental probabilities. Experiment 3 found that subjects’ preference for probability gain is robust, suggesting that the other models contribute little to subjects’ search behavior.
Effect of the addition of nanoclays on the water absorption and mechanical properties of glass fiber/up resin composites

Mechanical performance of composite materials must remain satisfactory during their life in service. However, composite structures such as wind blades are usually exposed to humid and severe environments, and the absorbed water can degrade the fiber–matrix interface decreasing the composite overall performance. Dual scale (nano-micro) composites can be obtained by adding nanoparticles to the polyester resin and reinforcing that nanomaterial with a glass fiber mat. These materials have good potential in the production of composite parts such as wind blades because nanoparticles dispersed in the polymer matrix could lower the water absorption of the composites and improve their performance in humid environments. However, nanosized reinforcing particles have high internal surface and therefore they tend to agglomerate and are difficult to disperse homogeneously in the polymeric matrix. Moreover, the final properties of the nanocomposites are strongly influenced by clay particles’ morphology and dispersion. In this work, the effect of the addition of pristine and organomodified nanoclays on the water absorption and mechanical properties of glass fiber/unsaturated polyester resin composites was studied. Results showed that the chemical treatments were effective in improving clay dispersion, reducing water absorption, and increasing the composites’ performance in humid environments. In addition, this work presents a procedure to use water absorption tests as means of accelerated humidity absorption tests.
Synergistic PET and SENSE MR Image Reconstruction Using Joint Sparsity Regularization

In this paper, we propose a generalized joint sparsity regularization prior and reconstruction framework for the synergistic reconstruction of positron emission tomography (PET) and under sampled sensitivity encoded magnetic resonance imaging data with the aim of improving image quality beyond that obtained through conventional independent reconstructions. The proposed prior improves upon the joint total variation (TV) using a non-convex potential function that assigns a relatively lower penalty for the PET and MR gradients, whose magnitudes are jointly large, thus permitting the preservation and formation of common boundaries irrespective of their relative orientation. The alternating direction method of multipliers (ADMM) optimization framework was exploited for the joint PET-MR image reconstruction. In this framework, the joint maximum a posteriori objective function was effectively optimized by alternating between well-established regularized PET and MR image reconstructions. Moreover, the dependency of the joint prior on the PET and MR signal intensities was addressed by a novel alternating scaling of the distribution of the gradient vectors. The proposed prior was compared with the separate TV and joint TV regularization methods using extensive simulation and real clinical data. In addition, the proposed joint prior was compared with the recently proposed linear parallel level sets (PLSs) method using a benchmark simulation data set. Our simulation and clinical data results demonstrated the improved quality of the synergistically reconstructed PET-MR images compared with the unregularized and conventional separately regularized methods. It was also found that the proposed prior can outperform both the joint TV and linear PLS regularization methods in assisting edge preservation and recovery of details, which are otherwise impaired by noise and aliasing artifacts. In conclusion, the proposed joint sparsity regularization within the presented a ADMM reconstruction framework is a promising technique, nonetheless our clinical results showed that the clinical applicability of joint reconstruction might be limited in current PET-MR scanners, mainly due to the lower resolution of PET images.
On the Identification of Cointegrated Systems in Small Samples: Practical Procedures with an Application to UK Wages and Prices

This paper discusses the practical application of identification in cointegrated systems. It will argue that in a common realistic modelling situation of a limited data set and the theory requirements of a fairly rich model, the techniques proposed in the existing literature are almost impossible to implement successfully. There are crucial decisions to be made over the order in which various restrictions are imposed in the move from a general unrestricted VECM to the fully (over) identified VECM. We will argue that imposing exogeneity restrictions at the earliest possible stage of the model reduction process and then restricting the dynamic adjustment of the model hugely increases the power of tests of overidentifying restrictions on the long run cointegrating vectors. These arguments are supported by Monte Carlo evidence. In practise this means that a thorough use of economic theory at an early stage, rather than treating a model as a pure statistical artifact, can yield enormous benefits.
Variability and interrelationships of grain quantity and quality characteristics in soybean

The objective of this study was to estimate the efficiency and reliability of grain quantity and quality characteristics in soybean (Glycine max (L.) Merrill) as selection criteria in breeding for higher grain yield. Trials were conducted on the experimental field of the Agricultural Institute Osijek (Croatia) during a three-year period (1993-1995) and involved 22 soybean genotypes. Mean values, variation coefficients, correlation coefficients and path coefficients were estimated for grain yield components, grain yield, protein and oil content in grain. The variation coefficient was low for the following traits: oil and protein content in grain, seed yield/plant, number of nodes/plant and seeds number/plant. Phenotypic path coefficients indicated a very strong positive direct effect of seeds number/plant and seed yield/plant on grain yield.The results of biometrical analyses showed that the direct selection on higher number of seeds/plant and higher seed yield/plant would result in greater genetic advance in grain yield.
Architecture of high-throughput context adaptive variable length coding decoder in AVC/H.264

In this paper, a High-throughput Context Adaptive Variable Length Coding decoder which is capable for supporting AVC/H.264 HP@level 4.2 has been presented. To increase throughput, multi-symbol decoders for “LEVEL” and “RunBefore” and architecture of fast zero insertion are presented to reduce processing cycles to reach high-throughput rate. Finally, the experimental results show that the throughput of presented Context Adaptive Variable Length Coding decoder achieves the level limitation of level 4.2 in AVC/H.264 and the synthesis result shows that the gate count is about 17.2K gates at a clock constrain of 108MHz.
Primary follicular thyroid carcinoma metastasis to the kidney and widespread dissemination: A case report

Distant metastases are more common in follicular thyroid carcinoma (FC) than in papillary thyroid carcinoma. However, FC metastasis to the kidney with eggshell calcification, as observed in the present case, is rare. The current report presents a case of a 67-year-old woman exhibiting a solitary tumor in the mid pole of the left kidney. Radical nephrectomy was performed, as the tumor was diagnosed as a primary renal carcinoma using contrast-enhanced computed tomography. Once the tumor was confirmed to be FC, total thyroidectomy was performed. Following administration of an oral therapeutic dose of 100 mCi 131I, functional imaging demonstrated the presence of multifocal metastases in the chest and abdomen. Euthyrox® was prescribed orally to aid normal thyroid function. Follow-up 6 months later using radionuclide imaging demonstrated the disappearance of the multifocal metastases in the chest and abdomen. The distant metastasis of FC may represent the initial symptom of the primary lesion, which was neglected. Ultrasound is an effective method to examine nodules located on the thyroid.
Solid Particle Erosion of Fiber Reinforced Plastics

Solid particle erosion tests were carried out to study the effect of matrix materials, reinforcement fibers, interface strength between matrix material and fibers, im pact angle, and particle velocity on the solid particle erosion behavior of fiber reinforced plastics. In the present study, special attention was focused on the effect of the interface strength between matrix material and fibers, which has not been investigated in a lot of previous works related with the erosion of composite materials. FRPs with treated or un treated fiber surfaces were used as test materials to study the effect of interface strength be tween matrix material and fibers. It is found from the erosion tests that the erosion rate is larger in a FRP than in a neat resin, and that the erosion rate of a FRP decreases with the increase of the interface strength between matrix material and fibers.
Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain.

AIM The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the elastic modulus and flexural strength of machined dentine bars, and (ii) changes in strain of 'whole' extracted human teeth were evaluated. One hundred standardized plano-parallel dentine bars (> 11.7 x 0.8 x 0.8 mm) were randomly divided into the three groups, immersed for 2 h in the respective solutions and then subjected to a three-point bend test. Changes in strain of each of 10 teeth on cyclical nondestructive occlusal loading were measured using electrical resistance strain gauges bonded to the cervical aspects. Each tooth had its crown and enamel reduced and root canal prepared. These were irrigated sequentially in a series of four separate, 30-minute regimes; initial-saline, 0.5% NaOCl, 5.25% NaOCl and final-saline. The changes in strains after each irrigation regime were compared. RESULTS There was a significant decrease in elastic modulus of the dentine bars immersed in 5.25% NaOCl compared with the saline group (P < 0.01). There was also a significant decrease in flexural strength of the dentine bars in the 5.25% NaOCl group compared to both the saline and 0.5% NaOCl groups (P < 0.01). The strain data from the nondestructive tooth loading tests revealed significant increases in tensile strain between the initial-saline and the final-saline stages (P < 0.01). Significant increases in compressive strains were also found between initial-saline and 5.25% NaOCl; and between 0.5% NaOCl and 5.25% NaOCl stages (P < 0.01). CONCLUSIONS The null hypothesis was rejected, 5.25% NaOCl reduced the elastic modulus and flexural strength of dentine. Irrigation of root canals of single, mature rooted premolars with 5.25% NaOCl affected their properties sufficiently to alter their strain characteristics when no enamel was present.
Functional Characterisation of Alpha-Galactosidase A Mutations as a Basis for a New Classification System in Fabry Disease

Fabry disease (FD) is an X-linked hereditary defect of glycosphingolipid storage caused by mutations in the gene encoding the lysosomal hydrolase α-galactosidase A (GLA, α-gal A). To date, over 400 mutations causing amino acid substitutions have been described. Most of these mutations are related to the classical Fabry phenotype. Generally in lysosomal storage disorders a reliable genotype/phenotype correlation is difficult to achieve, especially in FD with its X-linked mode of inheritance. In order to predict the metabolic consequence of a given mutation, we combined in vitro enzyme activity with in vivo biomarker data. Furthermore, we used the pharmacological chaperone (PC) 1-deoxygalactonojirimycin (DGJ) as a tool to analyse the influence of individual mutations on subcellular organelle-trafficking and stability. We analysed a significant number of mutations and correlated the obtained properties to the clinical manifestation related to the mutation in order to improve our knowledge of the identity of functional relevant amino acids. Additionally, we illustrate the consequences of different mutations on plasma lyso-globotriaosylsphingosine (lyso-Gb3) accumulation in the patients' plasma, a biomarker proven to reflect the impaired substrate clearance caused by specific mutations. The established system enables us to provide information for the clinical relevance of PC therapy for a given mutant. Finally, in order to generate reliable predictions of mutant GLA defects we compared the different data sets to reveal the most coherent system to reflect the clinical situation.
Non-steroidal anti-inflammatory agents for cystoid macular oedema following cataract surgery: a systematic review

Aim: To examine the effectiveness of non-steroidal anti-inflammatory drugs (NSAIDs) in the treatment of cystoid macular oedema (CMO) following cataract surgery. Methods: Systematic literature review of randomised controlled trials (RCTs) that evaluated the effects of NSAIDs in the treatment of CMO following cataract surgery was done according to the Cochrane Collaboration methodology. Results: Seven trials involving a total of 266 participants were included. Four trials studied the effects of NSAIDs in chronic CMO while the other three trials examined the effect of NSAIDs in acute CMO. Little evidence of effectiveness was found for oral indomethacin and topical fenoprofen for chronic CMO in two small trials. Treatment with topical 0.5% ketorolac for chronic CMO was found to be effective in two trials. Three trials examined the effect of topical NSAIDs on acute CMO. The comparisons among these studies were of a NSAID to placebo, prednisolone or another NSAID. Because of considerable heterogeneity between these study designs, their results were not combined in a meta-analysis. Conclusion: A positive effect of topical NSAID (0.5% ketorolac tromethamine ophthalmic solution) on chronic CMO was noted. However, there is not enough evidence to show the effectiveness of NSAIDs in acute CMO following cataract surgery.
A Space-For-Time (SFT) Substitution Approach to Studying Historical Phenological Changes in Urban Environment

Plant phenological records are crucial for predicting plant responses to global warming. However, many historical records are either short or replete with data gaps, which pose limitations and may lead to erroneous conclusions about the direction and magnitude of change. In addition to uninterrupted monitoring, missing observations may be substituted via modeling, experimentation, or gradient analysis. Here we have developed a space-for-time (SFT) substitution method that uses spatial phenology and temperature data to fill gaps in historical records. To do this, we combined historical data for several tree species from a single location with spatial data for the same species and used linear regression and Analysis of Covariance (ANCOVA) to build complementary spring phenology models and assess improvements achieved by the approach. SFT substitution allowed increasing the sample size and developing more robust phenology models for some of the species studied. Testing models with reduced historical data size revealed thresholds at which SFT improved historical trend estimation. We conclude that under certain circumstances both the robustness of models and accuracy of phenological trends can be enhanced although some limitations and assumptions still need to be resolved. There is considerable potential for exploring SFT analyses in phenology studies, especially those conducted in urban environments and those dealing with non-linearities in phenology modeling.
Emotion recognition using imperfect speech recognition

This paper investigates the use of speech-to-text methods for assigning an emotion class to a given speech utterance. Previous work shows that an emotion extracted from text can convey complementary evidence to the information extracted by classifiers based on spectral, or other non-linguistic features. As speech-to-text usually presents significantly more computational effort, in this study we investigate the degree of speechto-text accuracy needed for reliable detection of emotions from an automatically generated transcription of an utterance. We evaluate the use of hypotheses in both training and testing, and compare several classification approaches on the same task. Our results show that emotion recognition performance stays roughly constant as long as word accuracy doesn’t fall below a reasonable value, making the use of speech-to-text viable for training of emotion classifiers based on linguistics. Index Terms: speech-to-text, emotion detection, meta-data extraction, rich transcription, children’s speech
Contralateral Inhibition of Click- and Chirp-Evoked Human Compound Action Potentials

Cochlear outer hair cells (OHC) receive direct efferent feedback from the caudal auditory brainstem via the medial olivocochlear (MOC) bundle. This circuit provides the neural substrate for the MOC reflex, which inhibits cochlear amplifier gain and is believed to play a role in listening in noise and protection from acoustic overexposure. The human MOC reflex has been studied extensively using otoacoustic emissions (OAE) paradigms; however, these measurements are insensitive to subsequent “downstream” efferent effects on the neural ensembles that mediate hearing. In this experiment, click- and chirp-evoked auditory nerve compound action potential (CAP) amplitudes were measured electrocochleographically from the human eardrum without and with MOC reflex activation elicited by contralateral broadband noise. We hypothesized that the chirp would be a more optimal stimulus for measuring neural MOC effects because it synchronizes excitation along the entire length of the basilar membrane and thus evokes a more robust CAP than a click at low to moderate stimulus levels. Chirps produced larger CAPs than clicks at all stimulus intensities (50–80 dB ppeSPL). MOC reflex inhibition of CAPs was larger for chirps than clicks at low stimulus levels when quantified both in terms of amplitude reduction and effective attenuation. Effective attenuation was larger for chirp- and click-evoked CAPs than for click-evoked OAEs measured from the same subjects. Our results suggest that the chirp is an optimal stimulus for evoking CAPs at low stimulus intensities and for assessing MOC reflex effects on the auditory nerve. Further, our work supports previous findings that MOC reflex effects at the level of the auditory nerve are underestimated by measures of OAE inhibition.
The role of oxidative stress in the pathogenesis and treatment of asthma

The role of oxidative stress in asthma is gaining increasing scientific attention. The hallmark of asthma is airway inflammation. Oxidative stress may initiate and augment inflammation, and may also result from inflammation. Exposure to tobacco smoke, ozone, diesel exhaust, and a variety of other pollutants generates reactive oxygen species and other oxidative stressors. Some studies suggest that asthmatics have a decreased ability to respond to oxidative stress, while others find upregulated antioxidative function. Oxidative stress may alter the Th1/Th2 immune response and result in activation of NF‐kβ, a powerful inducer of pro‐inflammatory genes. Genetic polymorphisms may play an important role in determining susceptibility to oxidative stress. Many therapeutic strategies to decrease oxidative stress in asthma have been suggested. Dietary changes, antioxidant vitamins, other antioxidant drugs, Ayurvedic supplements, and even radon exposure in a hot bathroom have been studied. Minimizing exposure of young children to environmental tobacco smoke remains paramount.
Analyte-filled fiber core assisted surface plasmon sensing

A microstructured fiber based surface plasmon resonance sensor with a high sensitivity of 4500nm/RIU was proposed using an analyte-filled core instead of an air-filled core. Higher signal to noise ratio and narrower resonance spectral width were demonstrated and a better performance over the whole sensing range can be achieved.