Miracle-dz/UniFAR-FTUs huggingface dataset:
A coherence effect in multimedia learning: The case for minimizing irrelevant sounds in the design of multimedia instructional messages.

The authors tested the recommendation that adding bells and whistles (in the form of background music and/or sounds) would improve the quality of a multimedia instructional message. In 2 studies, students received an animation and concurrent narration intended to explain the formation of lightning (Experiment 1) or the operation of hydraulic braking systems (Experiment 2), For some students, the aathors added background music (Group NM), sounds (Group NS), bom (Group NSM), or neither (Group N). On tests of retention and transfer, Group NSM performed worse than Group N; groups receiving music performed worse than groups not receiving music; and groups receiving sounds performed worse (only in Experiment 2) man groups not receiving sounds. Results were consistent with the idea that auditory adjuncts can overload the learner's auditory working memory, as predicted by a cognitive theory of multimedia learning. Humans are designed to integrate multimodal stimuli into one meaningful experience, such as when they associate the sound of thunder to the visual image of lightning in the sky. However, when the process of lightning formation is to be taught using a computer, the instructional designer is faced with the need to choose between several alternative presentation formats to promote meaningful learning (Mayer & Moreno, 1998). Within the visual modality, for example, the process of lightning may be shown by a static diagram, an animation, or a video, and it may be described by visually presented text. Within the auditory modality, for example, the process of lightning may be accompanied by sound effects or background music, and it may be described by an auditorily presented narration. The present study examines one aspect of multimedia design, the role of auditory adjuncts such as background music and sound.
Development of Simplified Vasoactive Intestinal Peptide Analogs with Receptor Selectivity and Stability for Human Vasoactive Intestinal Peptide/Pituitary Adenylate Cyclase-Activating Polypeptide Receptors

Vasoactive intestinal peptide (VIP) is a widespread neurotransmitter whose physiological and pathophysiological actions are mediated by two receptor classes, VIP/pituitary adenylate cyclase-activating polypeptide (VPAC) 1 and VPAC2. VIP is a 28-amino acid peptide that is rapidly degraded and simplified; metabolically stable analogs are needed. In this study, we use information from studies of the VIP pharmacophore for VPAC1/VPAC2 to design nine simplified VIP analogs that could have high affinity and selectivity for each VPAC or that retained high affinity for both VPACs and were metabolically stable. From binding studies of their abilities to directly interact with hVPAC1 (T47D cells, hVPAC1-transfected cells) and hVPAC2 (Sup T1- and VPAC2-transfected cells) and to stimulate adenylate cyclase in each, two analogs [(Ala2,8,9,11,19,22,24,25,27,28)VIP and (Ala2,8,9,11,19,24–28)VIP] were found to have >2000- and >600-fold selectivity for hVPAC1. None of the nine analogs had hVPAC2 selectivity. However, two simplified analogs [(Ala2,8,9, 16,19,24)VIP and (Ala2,8,9,16,19,24,25)VIP] retained high affinity and potency for both hVPACs. 125I-[Ala2,8,9,16,19,24,25]VIP was much more metabolically stable than 125I-VIP. The availability of these simplified analogs of VIP, which are metabolically stable and have either hVPAC1 selectivity or retain high affinity for both hVPACs, should be useful for exploring the role of VPAC subtypes in mediating VIPs' actions as well as being useful therapeutically and for exploring the usefulness of VIP receptor imaging of tumors and VIP receptor-mediated tumor cytotoxicity.
Calmodulin confers calcium sensitivity on ciliary dynein ATPase

Extraction of demembranated cilia of Tetrahymena by Tris-EDTA (denoted by the suffix E) yields 14S-E and 30S-E dyneins with ATPase activities that are slightly increased by Ca++. This effect is moderately potentiated when bovine brain calmodulin is added to the assay mixture. Extraction with 0.5 M KCl (denoted by the suffix K) yeilds a 14S-K dynein with a low basal ATPase activity in the presence of Ca++. Subsequent addition of calmodulin causes marked activation (up to 10- fold) of ATPase activity. Although 14S-K and 14S-E dyneins have Ca++- dependent ATPase activities that differ markedly in the degree of activation, the concentration of calmodulin required for half-maximal saturation is similar for both, approximately 0.1 microM. Both 30S-K and 30S-E dyneins, however, require approximately 0.7 microM bovine brain calmodulin to reach half-maximal activation of their Ca++- dependent ATPase activities. Tetrahymena calmodulin is as effective as bovine brain calmodulin in activating 30S dynein , but may be slightly less effective than the brain calmodulin in activating 14S dynein. Rabbit skeletal muscle troponin C also activates the Ca++-dependent ATPase activity of 30S dynein and, to a lesser extent, that of 14S dynein, but in both cases is less effective than calmodulin. The interaction of calmodulin with dynein that results in ATPase activation is largely complete in less than 1 min, and is prevented by the presence of low concentrations of ATP. Adenylyl imidodiphosphate can partially prevent activation of dynein ATPase by calmodulin plus Ca++, but at much higher concentrations than required for prevention by ATP. beta, gamma-methyl-adenosine triphosphate appears not to prevent this activation. The presence of Ca++-dependent calmodulin-binding sites on 14S and 30S dyneins was demonstrated by the Ca++-dependent retention of the dyneins on a calmodulin-Sepharose-4B column. Gel electrophoresis of 14S dynein that had been purified by the affinity-chromatography procedure showed that presence of two major and one minor high molecular weight components. Similar analysis of 30S dynein purified by this procedure also revealed on major and one minor high molecular weight components that were different from the major components of 14S dynein. Ca++-dependent binding sites for calmodulin were shown to be present on axonemes that had been extracted twice with Tris-EDTA or with 0.5 M KCl by the use of 35S-labeled Tetrahymena calmodulin. It is concluded that the 14S and 30S dyneins of Tetrahymena contain Ca++- dependent binding sites for calmodulin and the calmodulin mediates the Ca++-regulation of the dynein ATPases of Tetrahymena cilia.
A New Agility Test for Adults: Its Test–Retest Reliability and Minimal Detectable Change in Untrained Women and Men Aged 28–55

Abstract Manderoos, SA, Vaara, ME, Mäki, PJ, Mälkiä, EA, Aunola, SK, and Karppi, S-L. A new agility test for adults: its test–retest reliability and minimal detectable change in untrained women and men aged 28−55. J Strength Cond Res 30(8): 2226–2234, 2016—The aims of this study were to present a new Agility Test for Adults (ATA), to investigate its test–retest reliability and to quantify minimal detectable change at the 95% confidence interval (MDC95). Both the relative and absolute reliabilities were evaluated. Altogether 52 healthy untrained volunteers (25 women: age 43.3 ± 6.6 years; 27 men: age 42.8 ± 7.2 years) were recruited into the study. The subjects performed 3 ATA tests repeated after 2 different intervals: the first test session was baseline, session 2 was a week later, and session 3 was half an hour after session 2. The intraclass correlation coefficient and the SEM of the performance time of ATA were 0.91 and 0.27 seconds (same day), 0.94 and 0.20 seconds (1 week) for women, and 0.95, 0.13 seconds, and 0.94, 0.19 seconds for men, respectively. MDC95 was 0.76 seconds (same day) and 0.56 seconds (1 week) for women, and respectively 0.37 and 0.51 seconds for men. The results showed that ATA is stable and reliable when evaluating agility characteristics in untrained adults. The properties of ATA make it appropriate for screening people to find early signs of declined agility and allow possibility to clinicians and physical trainers to monitor true changes in performance time at agility test by applying the knowledge of MDC95 coefficient. Furthermore, ATA can give tips for planning appropriate exercise programes to prevent clumsiness and falls with more serious consequences among aging people.
Unique-Word GFDM Transmission Systems

In this letter, we propose the use of a deterministic sequence, known as unique word (UW), instead of the cyclic prefix in generalized frequency division multiplexing (GFDM) systems. The UW consists of known sequences that, if not null, can be used advantageously for synchronization and channel estimation purposes. In addition, UW allows the application of a highly efficient linear minimum mean squared error (LMMSE) smoother for noise reduction at the receiver. To avoid the conditions of non-orthogonality caused by the insertion of the UW and performance degradation in time varying frequency-selective channels, we use frequency-shift offset quadrature amplitude modulation. We present a signal model of a UW-GFDM system considering a single and multiple UWs. We then develop an LMMSE receive filter for signal reception of the proposed UW-GFDM system. Simulations show that the proposed UW-GFDM system outperforms prior work.
Systematics and Biogeography of the Silverside Tribe Menidiini (Teleostomi: Atherinopsidae) Based on the Mitochondrial ND2 Gene

Abstract The silverside fish tribe Menidiini (Teleostei: Atherinopsidae) consists of four genera, Menidia, Labidesthes, Poblana, and Chirostoma, that are distributed along the Atlantic coast of North America, throughout the Gulf of México, insular United States, and the Mesa Central of México. It has been suggested that Chirostoma, Poblana, and Menidia should be recognized as a single genus under the nominal Menidia. To test this hypothesis, phylogenetic relationships within the tribe Menidiini were assessed using the mitochondrially encoded ND2 gene. Monophyly of the Menidiini tribe was supported. Results also failed to support monophyly for the genera Menidia and Chirostoma as currently recognized. A central Mexican clade, inclusive of Chirostoma and Poblana, was recovered as monophyletic and strongly supported. Relationships within the Mesa Central clade support a previously recognized “humboldtianum” clade and the paraphyly of Chirostoma with respect to Poblana.
Mediterranean diet and metabolic syndrome: the evidence

Abstract Background The Mediterranean diet has long been related to a lower cardiovascular disease risk; however, more recent evidences also indicate that it has a favourable effect on adiposity and type 2 diabetes. Design Review of the available literature in relation to Mediterranean diet and metabolic syndrome. Results Several components of Mediterranean diet patterns have been inversely related with body mass index. They are considered to be modulators of insulin resistance, can exert beneficial effects on blood pressure, improve atherogenic dyslipidemia or attenuate the inflammatory burden associated with metabolic syndrome. Furthermore, a lower prevalence of metabolic syndrome has been associated with dietary patterns rich in fruits and vegetables, nuts, olive oil, legumes and fish, moderate in alcohol and low in red meat, processed meat, refined carbohydrates and whole-fat dairy products. Conclusions There is much evidence suggesting that the Mediterranean diet could serve as an anti-inflammatory dietary pattern, which could help to fight diseases related to chronic inflammation, including metabolic syndrome.
Phosphoprotein Analysis Using Antibodies Broadly Reactive against Phosphorylated Motifs*

The substrates of most protein kinases remain unknown because of the difficulty tracing signaling pathways and identifying sites of protein phosphorylation. Here we describe a method useful in detecting subclasses of protein kinase substrates. Although the method is broadly applicable to any protein kinase for which a substrate consensus motif has been identified, we illustrate here the use of antibodies broadly reactive against phosphorylated Ser/Thr-motifs typical of AGC kinase substrates. Phosphopeptide libraries with fixed residues corresponding to consensus motifs RXRXXT*/S* (Akt motif) and S*XR (protein kinase C motif) were used as antigens to generate antibodies that recognize many different phosphoproteins containing the fixed motif. Because most AGC kinase members are phosphorylated and activated by phosphoinositide-dependent protein kinase-1 (PDK1), we used PDK1−/− ES cells to profile potential AGC kinase substrates downstream of PDK1. To identify phosphoproteins detected using the Akt substrate antibody, we characterized the antibody binding specificity to generate a specificity matrix useful in predicting antibody reactivity. Using this approach we predicted and then identified a 30-kDa phosphoprotein detected by both Akt and protein kinase C substrate antibodies as S6 ribosomal protein. Phosphospecific motif antibodies offer a new approach to protein kinase substrate identification that combines immunoreactivity data with protein data base searches based upon antibody specificity.
The diagnostic accuracy of two tense measures for identifying 3-year-olds with language impairment.

PURPOSE The authors of this study investigated the diagnostic accuracy of the Finite Verb Morphology Composite (FVMC; Bedore & Leonard, 1998) and the Tense and Agreement Productivity Score (TAPS; Hadley & Short, 2005) in identifying 3-year-olds with language impairment (LI). METHOD Eighteen pairs of 3-year-olds with and without LI participated in the current study. The FVMC and the TAPS were computed from 100- and 50-utterance language samples. RESULTS The FVMC and TAPS demonstrated higher diagnostic accuracy in the 100-utterance samples than in the 50-utterance samples. For 100-utterance samples, when children's age or severity was not considered, the FVMC showed a slight advantage over the TAPS in diagnostic accuracy. However, when children's age or severity was considered, the opposite pattern was observed. CONCLUSION Both the FVMC and TAPS can be used to differentiate 3-year-olds with and without LI. To reliably identify 3-year-olds with LI by using tense measures, language samples with at least 100 utterances are recommended.
Permaculture Adoption Among Malawian Farmers: A Positive Deviance Inquiry

iv Introduction 1 1. Background 1 A. Snapshot Malawi 5 B. Contemporary Agriculture versus Permaculture: Malawi Context 6 2. Statement of Research Question 12 Literature Review 14 1. Positive Deviance 14 2. Adoption of Agricultural Techniques and Practices 17 Research Methodology 20 Presentation and Analysis of Data 27 1. Profile of the Respondents 27 2. Adoption 31 3. Yields 44 4. Food and Nutrition Security Score 46 Discussion & Conclusions 52 Bibliography 58 Appendix 61
Rigidity of graded regular algebras

We prove a graded version of Alev-Polo's rigidity theorem: the homogenization of the universal enveloping algebra of a semisimple Lie algebra and the Rees ring of the Weyl algebras A n (k) cannot be isomorphic to their fixed subring under any finite group action. We also show the same result for other classes of graded regular algebras including the Sklyanin algebras.
Wavelet Representation of the Corneal Pulse for Detecting Ocular Dicrotism

Purpose To develop a reliable and powerful method for detecting the ocular dicrotism from non-invasively acquired signals of corneal pulse without the knowledge of the underlying cardiopulmonary information present in signals of ocular blood pulse and the electrical heart activity. Methods Retrospective data from a study on glaucomatous and age-related changes in corneal pulsation [PLOS ONE 9(7),(2014):e102814] involving 261 subjects was used. Continuous wavelet representation of the signal derivative of the corneal pulse was considered with a complex Gaussian derivative function chosen as mother wavelet. Gray-level Co-occurrence Matrix has been applied to the image (heat-maps) of CWT to yield a set of parameters that can be used to devise the ocular dicrotic pulse detection schemes based on the Conditional Inference Tree and the Random Forest models. The detection scheme was first tested on synthetic signals resembling those of a dicrotic and a non-dicrotic ocular pulse before being used on all 261 real recordings. Results A detection scheme based on a single feature of the Continuous Wavelet Transform of the corneal pulse signal resulted in a low detection rate. Conglomeration of a set of features based on measures of texture (homogeneity, correlation, energy, and contrast) resulted in a high detection rate reaching 93%. Conclusion It is possible to reliably detect a dicrotic ocular pulse from the signals of corneal pulsation without the need of acquiring additional signals related to heart activity, which was the previous state-of-the-art. The proposed scheme can be applied to other non-stationary biomedical signals related to ocular dynamics.
Flexible band versus rigid ring annuloplasty for tricuspid regurgitation: a systematic review and meta-analysis.

BACKGROUND Up to 20% of patients have pre-discharge residual moderate to severe tricuspid regurgitation (TR) after tricuspid repair. Reoperations for recurrent TR carry high mortality rates, which emphasizes the importance of identifying the optimal technique for the surgical management of TR. The present study is a systematic review and meta-analysis that aims to compare short and long term survival and freedom from TR of flexible band ring versus rigid ring for annuloplasty of TR. METHODS We conducted a systematic review and meta-analysis of comparative studies to evaluate these procedures. A systematic search of the literature was performed from six electronic databases. Pooled meta-analysis was conducted using odds ratio (OR) and weighted mean difference (WMD). RESULTS The rates of in-hospital mortality were not different between the two groups, with cumulative rates of 6.9% for flexible band and 7.3% for rigid ring (OR: 0.92; 95% CI: 0.49-1.71). Rates of stroke were also similar with 1.7% of flexible band and 1.3% of rigid rings suffering a perioperative stroke (OR: 1.29; 95% CI: 0.74-2.23). Rigid ring had significantly better freedom from grade ≥2 TR at 5 years (OR: 0.44; 95% CI: 0.20-0.99) and overall (P=0.005). There was no significant difference in overall rates of reoperation (P=0.232) and survival (P=0.086) between flexible band and rigid ring. CONCLUSIONS Both rigid ring and flexible band offer acceptable outcomes for the treatment of TR. Compared to flexible band, rates of TR are stable after rigid ring annuloplasty and long term freedom from TR are superior for rigid ring devices. Large prospective randomized trials are required in order to validate these findings and assess for improvements in patient survival.
Virulence genes promote conjugative transfer of the Ti plasmid between Agrobacterium strains

Certain virulence region operons of the Agrobacterium tumefaciens Ti plasmid promoted conjugative Ti plasmid transfer. Mutations in the vir region of pTiC58 inhibited conjugative plasmid transfer between A. tumefaciens strains. Mutations in virA, virG, 5' virB, and virE had the greatest effect on plasmid transfer, and mutations in virC had no effect. Transfer inhibition in vir mutants occurred in the presence or absence of acetosyringone.
Efficient gene delivery system mediated by cis-aconitate-modified chitosan-g-stearic acid micelles

Cis-aconitate-modified chitosan-g-stearic acid (CA-CSO-SA) micelles were synthesized in this study to improve the gene transfection efficiency of chitosan-g-stearic acid (CSO-SA). The CA-CSO-SA micelles had a similar size, critical micelle concentration, and morphology, but their zeta potential and cytotoxicity were reduced compared with CSO-SA micelles. After modification with cis-aconitate, the CA-CSO-SA micelles could also compact plasmid DNA (pDNA) to form nanocomplexes. However, the DNA binding ability of CA-CSO-SA was slightly reduced compared with that of CSO-SA. The transfection efficiency mediated by CA-CSO-SA/pDNA against HEK-293 cells reached up to 37%, and was much higher than that of CSO-SA/pDNA (16%). Although the cis-aconitate modification reduced cellular uptake kinetics in the initial stages, the total amount of cellular uptake tended to be the same after 24 hours of incubation. An endocytosis inhibition experiment showed that the internalization mechanism of CA-CSO-SA/pDNA in HEK-293 cells was mainly via clathrin-mediated endocytosis, as well as caveolae-mediated endocytosis and macropinocytosis. Observation of intracellular trafficking indicated that the CSO-SA/pDNA complexes were trapped in endolysosomes, but CA-CSO-SA/pDNA was more widely distributed in the cytosol. This study suggests that modification with cis-aconitate improves the transfection efficiency of CSO-SA/pDNA.
Randomized competitive algorithms for generalized caching

We consider online algorithms for the generalized caching problem. Here we are given a cache of size k and pages with arbitrary sizes and fetching costs. Given a request sequence of pages, the goal is to minimize the total cost of fetching the pages into the cache. We give an online algorithm with competitive ratio O(log2k), which is the first algorithm for the problem with competitive ratio sublinear in k. We also give improved O(log k)-competitive algorithms for the special cases of the Bit Model and Fault model. In the Bit Model, the fetching cost is proportional to the size of the page and in the Fault model all fetching costs are uniform. Previously, an O(log2 k)-competitive algorithm due to Irani [14] was known for both of these models. Our algorithms are based on an extension of the primal-dual framework for online algorithms which was developed by Buchbinder and Naor [7]. We first generate an O(log k)-competitive fractional algorithm for the problem. This is done by using a strengthened LP formulation with knapsack-cover constraints, where exponentially many constraints are added upon arrival of a new request. Second, we round online the fractional solution and obtain a randomized online algorithm. Our techniques provide a unified framework for caching algorithms and are substantially simpler than those previously used.
Improved Repetitive-Element PCR Fingerprinting ofSalmonella enterica with the Use of Extremely Elevated Annealing Temperatures

ABSTRACT Modified thermal cycling conditions were explored in an effort to improve the reproducibility and resolving power of repetitive-element PCR (rep-PCR) fingerprinting. Assay performance was rigorously evaluated under standard and modified cycling conditions, using as a test set 12 strains putatively representing 12 serovars ofSalmonella enterica. For all three fingerprint types (ERIC2, BOXA1R, and composite fingerprints), the use of extremely elevated annealing temperatures plus an initial “touchdown” cycling routine yielded significant improvements in day-to-day reproducibility and discriminating power despite the somewhat sparser appearance of the fingerprints. Modified cycling conditions markedly reduced the variability of fingerprints between cyclers, allowing fingerprints from different cyclers to be analyzed together without the degradation of assay performance that occurred with between-cycler analyses under standard cycling conditions. With modified cycling, composite fingerprints exhibited the lowest reproducibility but the highest net discriminating power of the three fingerprint types. rep-PCR fingerprints led to the discovery of a serotyping error involving one of the 12 test strains. These data demonstrate that modified cycling regimens that incorporate elevated annealing temperatures (with or without an initial touchdown routine) may markedly improve the performance of rep-PCR fingerprinting as a bacterial typing tool.