Miracle-dz/UniFAR-FTUs huggingface dataset:
Environmental Toxicology and Chemistry

Sediment toxicity is usually assessed by conducting laboratory bioassays on thoroughly homogenized, field‐ collected, sediment samples. Although it is generally held that these bioassays provide a conservative assessment of the potential for environmental impact, there are few studies comparing the results of laboratory sediment bioassays with actual measures of benthic community health in exposed field populations. To help inform an understanding of the relative efficacy of laboratory‐based bioassays in predicting potential impacts in exposed field populations, a laboratory‐to‐field comparison study was conducted. Laboratory bioassays included standard 10‐d acute toxicity tests measuring survival in 4 species of estuarine/marine amphipods (Eohaustorius estuarius, Ampelisca abdita, Rhepoxinius abronius, and Leptocheirus plumulosus) and 2 longer term, 28‐d sublethal tests with a marine polychaete, Neanthes arenaceodentata (survival and growth), and the amphipod L. plumulosus (survival, growth, and reproduction). A highly contaminated and toxic sediment was mixed with a cleaner sediment of similar grain size to produce a series of diluted contaminated sediment treatments (0, 6, 12, 25, and 50%). Sediment treatments were placed in containers and deployed in the field. At specified intervals (at time of deployment and 9 and 12 mo post deployment), containers were retrieved from the field and analyzed for sediment chemistry, infaunal community composition, and toxicity. Laboratory toxicity endpoints were compared with measures of benthic community health to evaluate the ability of the toxicity tests to accurately predict benthic impacts. The results of these comparisons indicate that the laboratory tests evaluated provide conservative estimates of potential benthic community impacts, with both acute and chronic tests detecting effects at lower treatment levels than were detected in exposed field populations using traditional measures of benthic community health. Environ Toxicol Chem 2019;38:1784–1802. Published 2019 Wiley Periodicals, Inc. on behalf of SETAC. This article is a US government work, and as such, is in the public domain in the United States of America.
Influence of medium composition on the growth and antigen expression of Helicobacter pylori

An evaluation of the ability of various solid and liquid media to support both growth and antigen expression, particularly lipopolysaccharide (LPS) expression, by Helicobacterpylori culture collection strains and clinical isolates was performed. Liquid‐based basal media (brain heart infusion, Brucella broth, Mueller–Hinton broth and tryptone soya broth) supported the growth of strains, whereas solid basal media of the same formulation did not support growth. Optimal growth of all strains was obtained on solid and in liquid media containing blood. Supplemented solid media containing supplements other than blood supported growth but only to a small extent. In liquid media excluding blood, serum supplements enhanced growth and horse serum was found to be superior to fetal calf serum. In general, β‐cyclodextrin did not increase growth. Mueller–Hinton broth or tryptone soya broth containing horse serum and a nitrogen source such as yeast extract or proteose peptone no. 3 were found to give optimal growth of H. pylori in a blood‐free environment. Strains after cultivation in liquid media, irrespective of composition, maintained production of high‐molecular weight (mol. wt) LPS with an O side chain independent of medium composition, whereas subculturing on solid media resulted in production of low‐mol. wt LPS. Expression of proteins differed in liquid and on solid media, particularly proteins of 57 and 60 kDa, but qualitatively no differences were observed upon supplementation of basal media.
Prognostic Factors of Long-Term Hearing Preservation in Small and Medium-Sized Vestibular Schwannomas After Microsurgery.

OBJECTIVE The authors evaluated the long-term hearing outcomes of patients with vestibular schwannoma (VS) to explore appropriate surgical treatment. STUDY DESIGN Retrospective study. SETTING Tertiary referral center. PATIENTS A total of 138 patients diagnosed with small and medium-sized VS with serviceable hearing from January 2006 to December 2015. INTERVENTIONS All patients underwent microsurgery via retrosigmoid (RSA) or middle cranial fossa approach (MFA) and were followed up for over 2 years. MAIN OUTCOME MEASURES Pre- and postoperative hearing, including pure tone audiometry, speech discrimination score, and auditory brainstem response (ABR), were analyzed. RESULTS The mean tumor size and volume were 16.6 ± 3.4 mm and 1711.8 ± 918.5 mm, respectively. Preoperative hearing levels were Class A in 42, Class B in 67, and Class C in 29 patients. Patients with a tumor from the superior vestibular nerve (SVN) had better hearing at diagnosis. Postoperative hearing levels were Class A, B, C, and D for 28, 17, 32, and 61 patients. Hearing outcomes were significantly better in patients with normal intraoperative I wave on ABR. Hearing loss within 6 months had a positive effect on postoperative hearing. Better preoperative hearing and tumors from SVN were correlated with better postoperative hearing outcomes. Tumor size, cystic variation, or extension to the fundus of internal auditory canal had no influence on hearing preservation. CONCLUSIONS Better preoperative hearing, shorter hearing loss period, tumors from SVN, and normal intraoperative I wave are prognostic factors for serviceable hearing. RSA and MFA are effective and safe for tumor removal and hearing preservation.
Posttreatment Effect of MGMT Methylation Level on Glioblastoma Survival.

The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) removes temozolomide-induced alkylation, thereby preventing DNA damage and cytotoxicity. We investigated the prognostic effect of different MGMT methylation levels on overall and progression-free survival in 327 patients with primary glioblastoma undergoing standard treatment. We obtained MGMT methylation level in 4 CpG sites using pyrosequencing. The association between MGMT methylation level and survival was investigated using Cox proportional hazards model and an extension to detect time-varying effects. We found an association between MGMT methylation level and overall survival (OS) from around 9 months after the diagnosis, with no association between MGMT methylation level and OS before that. For patients surviving at least 9 months even small increases in MGMT methylation level are significantly beneficial (HR = 0.97, 95% CI [0.96, 0.98]). The predictive ability of MGMT methylation level on OS from 9 months after diagnosis has a Harrel's C of 66%. We conclude that the MGMT methylation level is strongly associated with survival only for patients surviving beyond 9 months with considerable effects for levels much lower than previously reported. Prognostic evaluation of cut-points of MGMT methylation levels and of CpG island site selection should take the time-varying effect on overall survival into account.
Maximum-likelihood estimation of specific differential phase and attenuation in rain

Precise estimation of propagation parameters in precipitation media is of interest to improve the performance of communications systems and in remote sensing applications. In this paper, we present maximum-likelihood estimators of specific attenuation and specific differential phase in rain. The model used for obtaining the cited estimators assumes coherent propagation, reflection symmetry of the medium, and Gaussian statistics of the scattering matrix measurements. No assumptions about the microphysical properties of the medium are needed. The performance of the estimators is evaluated through simulated data. Results show negligible estimators bias and variances close to Cramer-Rao bounds.
Experiencing community and domestic violence is associated with epigenetic changes in DNA methylation of BDNF and CLPX in adolescents

Abstract Experiencing violence changes behavior, shapes personalities, and poses a risk factor for mental disorders. This association might be mediated through epigenetic modifications that affect gene expression, such as DNA methylation. The present study investigated the impact of community and domestic violence on DNA methylation measured in saliva collected from 375 individuals including three generations: grandmothers (n = 126), mothers (n = 125), and adolescents (n = 124, 53% female). Using the Infinium HumanMethylation450 BeadChip array, in adolescents, we detected two CpG sites that showed an association of DNA methylation and lifetime exposure to community and domestic violence even after FDR correction: BDNF_cg06260077 (logFC −0.454, p = 3.71E‐07), and CLPX_cg01908660 (logFC = −0.372, p = 1.38E‐07). Differential DNA methylation of the CpG BDNF_cg06260077 associated with exposure to violence was also observed in the maternal but not the grandmaternal generation. BDNF (brain‐derived neurotrophic factor) and CLPX (caseinolytic mitochondrial matrix peptidase chaperone subunit) genes are involved in neural development. Our results thus reveal altered molecular mechanisms of developmental and intergenerational trajectories in survivors of repeated violent experiences.
miR-216a exacerbates TGF-β-induced myofibroblast transdifferentiation via PTEN/AKT signaling

Myofibroblast transdifferentiation is an important feature of cardiac fibrosis. Previous studies have indicated that microRNA-216a (miR-216a) is upregulated in response to transforming growth factor-β (TGF-β) in kidney cells and can activate Smad3; however, its role in myofibroblast transdifferentiation remains unclear. The present study aimed to investigate the role of miR-216a in TGF-β-induced myofibroblast transdifferentiation, and to determine the underlying mechanisms. Adult mouse cardiac fibroblasts were treated with TGF-β to induce myofibroblast transdifferentiation. An antagomir and agomir of miR-216a were used to inhibit or overexpress miR-216a in cardiac fibroblasts, respectively. Myofibroblast transdifferentiation was evaluated based on the levels of fibrotic markers and α-smooth muscle actin expression. The miR-216a antagomir attenuated, whereas the miR-216a agomir promoted TGF-β-induced myofibroblast transdifferentiation. Mechanistically, miR-216a accelerated myofibroblast transdifferentiation via the AKT/glycogen synthase kinase 3β signaling pathway, independent of the canonical Smad3 pathway. In addition, it was observed that miR-216a activated AKT via the downregulation of PTEN. In conclusion, miR-216a was involved in the regulation of TGF-β-induced myofibroblast transdifferentiation, suggesting that targeting miR-216a may aid in developing effective interventions for the treatment of cardiac fibrosis.
Vitexin alleviates interleukin‐1β‐induced inflammatory responses in chondrocytes from osteoarthritis patients: Involvement of HIF‐1α pathway

It has been reported that vitexin has anti‐inflammatory effects in osteoarthritis (OA) rats. However, the effects of vitexin on interleukins‐1β (IL‐1β)‐stimulated OA patient‐derived chondrocytes have not been reported. The purpose of this study was to investigate the anti‐inflammatory effects of vitexin on IL‐1β‐stimulated human osteoarthritis chondrocytes and to reveal the involvement of hypoxia‐inducible factor 1α (HIF‐1α) pathway. Enzyme‐linked immunosorbent assay, quantitative real‐time PCR and Western blotting assays were employed. ELISA results demonstrated that the proinflammatory cytokine levels of interleukins‐6 (IL‐6) and tumour necrosis factor α (TNF‐α) in the serum and synovial fluid and HIF‐1α level in the synovial fluid were significantly elevated in OA patients compared to normal healthy subjects. Moreover, the Western blotting results indicated that the protein expression of HIF‐1α was significantly higher in the cartilage tissues of OA patients. OA patient‐derived chondrocytes were stimulated by IL‐1β and treated with different concentration of vitexin for 24 hours. Vitexin showed no cytotoxicity and increased the survival of chondrocytes under IL‐1β stimulation. Vitexin suppressed IL‐1β‐induced production of NO and prostaglandin E2 (PGE2) in chondrocytes culture. The treatment of vitexin significantly inhibited IL‐1β‐induced expressions of proinflammatory cytokine levels of IL‐6, TNF‐α, matrix metalloproteinase (MMP)‐1, MMP‐3 and MMP‐13. Furthermore, Western blotting results demonstrated that HIF‐1α is involved in vitexin's protective effects on IL‐1β‐stimulated injuries in OA patient‐derived chondrocytes. Our study demonstrates that vitexin alleviates IL‐1β‐induced inflammatory responses in chondrocytes from osteoarthritis patients, which may be attributed partly to the inhibition of HIF‐1α pathway.
Overactivation of the Reward System and Deficient Inhibition in Exercise Addiction

ABSTRACT Purpose Behavior studies have found that exercise addiction is associated with high impulsivity. In other addictions, neural mechanisms of impulsivity reflect abnormalities in the reward and inhibition systems. In this study, we determined whether abnormalities existed in the reward and inhibition systems of exercise addicts. Methods Three groups of male participants (15 exercise addicts, 18 regular exercisers, and 16 exercise avoiders) completed the Mini International Personality Item Pool (Mini-IPIP), the classic go/no-go task, and the exercise-related go/no-go task. Event-related potentials (ERP) were recorded during the go/no-go tasks, and correctly performed trials were analyzed. Results Exercise addicts scored lower for extraversion and higher for neuroticism, reflecting a poor capacity for emotional regulation and impulse control, and had larger N2 and P3d amplitudes during the exercise-related go/no-go task. Exercise addicts and exercise avoiders demonstrated impaired accuracy in the exercise-related go/no-go task and had larger N2 amplitudes compared with regular exercisers during the letter–digit go/no-go task. Exercise addicts and regular exercisers showed larger Go-N1 and Go-P2 amplitudes compared with exercise avoiders during the exercise-related go/no-go task. Exercisers (exercise addicts and regular exercisers) demonstrated higher activation in response to exercise-related stimuli as reflected by larger N1 and P2, and addicts (exercise addicts) demonstrated poorer inhibition as reflected by larger N2 and P3d amplitudes. Go-N1 and Go-P2 were significantly correlated with no-go accuracy in exercise-related task. Conclusions Exercise addicts scored higher for the neuroticism personality trait and exhibited overactivation of the reward system and underactivation of the inhibition system. Overactivation of the reward system may be related to long-term exposure to exercise. Underactivation of the inhibition system may be a crucial factor in exercise addiction.
Premenstrual syndrome is associated with altered cortisol awakening response

Abstract Previous studies have revealed stress-induced dysregulation of hypothalamic-pituitary-adrenal (HPA) axis in women with premenstrual syndrome (PMS). So far, however, the results about the relationship between HPA axis dysregulation and PMS are mixed. To this end, it is necessary to investigate the basal activity of the HPA axis in women with PMS instead of only assessing a certain stressor. Therefore, this study evaluated the relationship between the cortisol awakening response (CAR) and PMS. Thirty-two women with PMS (mean age 22.47 ± 2.20 years) and 36 healthy controls (mean age 22.28 ± 2.43 years) were included in this study. Saliva samples of our participants were collected successively at 0, 30, 45, and 60 min after awakening to assess CAR during each of two phases of the menstrual cycle (the mid-follicular phase and the late luteal phase). The results showed a significantly attenuated CAR in women with PMS compared with the healthy controls, especially at 45 and 60 min after awakening, regardless of the menstrual cycle phases. Furthermore, there was a significant negative correlation between PMS severity as measured by PMS scale and AUCi (i.e. the Area Under the Curve with respect to increase) in the mid-follicular phase. Our findings suggested that an attenuated CAR activity profile may be an important risk factor for the development of PMS.
Abusive supervision and suicidal ideation: The mediating role of basic psychological need satisfaction

Abstract This study examines whether abusive supervision is related to suicide ideation and whether the need for autonomy, competence, and relatedness mediates this relationship. We use a diverse sample (N = 687) from six Chinese organizations. Abusive supervision was significantly related to suicidal ideation and satisfaction of the needs for competence and relatedness, but not for autonomy, partially mediated this relationship. Findings suggest that organizations that initiate intervention strategies to limit the adverse consequences of abusive supervision thereby help protect subordinates from suicidal ideation.
Multiple magnetic dipolarizations observed by THEMIS during a substorm

Abstract. The magnetic field dipolarization in the vicinity of substorm onset and during substorm expansion phase during the period of 06:00–06:40 UT on 15 February 2008 is investigated with observations from multiple probes of THEMIS. It is found that the magnetic dipolarization at the substorm onset (the onset time was about 06:14 UT) was not accompanied by obvious magnetic disturbance and ion bulk speed variation. The magnetic dipolarizations taking place during the substorm expansion phase observed by P4~(−10.97, 2.04, −3.03) RE and P3~(−11.32, 1.15, −3.10) RE were mostly accompanied by high speed earthward ion bulk flow, but the magnetic dipolarizations occurring during the substorm expansion phase observed by P5~(−9.45, 1.07, −2.85) RE were not accompanied by high speed earthward ion bulk flow. Before substorm onset THEMIS P3, P4, P5 all observed the Bx component fluctuation with a period of about 300 s. After substorm onset earthward high speed ion bulk flow and significant magnetic disturbances both occurred at P3 and P4 locations. These results indicate that there is no one-to-one relationship between the near-Earth magnetic dipolarization and the earthward ion bulk flow. In particular, the magnetic dipolarization occurring on the earthward side of the inner near-Earth plasma sheet is not accompanied by high speed earthward ion bulk flow. The dipolarization at substorm onset is a local and small scale phenomenon. There are multiple magnetic dipolarizations occurring during the substorm expansion phase. The dipolarization process is very complex and is not simply an MHD process. It is accompanied by some kinds of plasma instabilities, the plasma sheet azimuthal expansion not only by earthward ion bulk flow during substorm. A sharp increase of the AE index does not always give an accurate substorm onset time for substorm analysis.
Translating Research on Myoelectric Control into Clinics—Are the Performance Assessment Methods Adequate?

Missing an upper limb dramatically impairs daily-life activities. Efforts in overcoming the issues arising from this disability have been made in both academia and industry, although their clinical outcome is still limited. Translation of prosthetic research into clinics has been challenging because of the difficulties in meeting the necessary requirements of the market. In this perspective article, we suggest that one relevant factor determining the relatively small clinical impact of myocontrol algorithms for upper limb prostheses is the limit of commonly used laboratory performance metrics. The laboratory conditions, in which the majority of the solutions are being evaluated, fail to sufficiently replicate real-life challenges. We qualitatively support this argument with representative data from seven transradial amputees. Their ability to control a myoelectric prosthesis was tested by measuring the accuracy of offline EMG signal classification, as a typical laboratory performance metrics, as well as by clinical scores when performing standard tests of daily living. Despite all subjects reaching relatively high classification accuracy offline, their clinical scores varied greatly and were not strongly predicted by classification accuracy. We therefore support the suggestion to test myocontrol systems using clinical tests on amputees, fully fitted with sockets and prostheses highly resembling the systems they would use in daily living, as evaluation benchmark. Agreement on this level of testing for systems developed in research laboratories would facilitate clinically relevant progresses in this field.
Distribution-Free Probability Density Forecast Through Deep Neural Networks

Probability density forecast offers the whole distributions of forecasting targets, which brings greater flexibility and practicability than the other probabilistic forecast models such as prediction interval (PI) and quantile forecast. However, existing density forecast models have introduced various constraints on forecasted distributions, which has limited their ability to approximate real distributions and may result in suboptimality. In this paper, a distribution-free density forecast model based on deep learning is proposed, in which the real cumulative density functions (CDFs) of forecasting target are approximated by a large-capacity positive-weighted deep neural network (NN). Benefiting from the universal approximation ability of NNs, the range of forecasted distributions has been proven to contain all the distributions with continuous CDFs, which is superior to existing models’ considering both width and accordance with reality. Three tests from different scenarios were implemented for evaluation, i.e., very-short-term wind power, wind speed, and day-ahead electricity price forecast, in which the proposed density forecast model has shown superior performance over the state of the art.
Vasoactive Agents for Adult Septic Shock: An Update and Review

Purpose The objective of this article is to discuss the pharmacology, side effects, and clinical application of vasoactive therapy in the management of adult septic shock. Summary Sepsis is one of the most common reasons for admission to an intensive care unit with the incidence estimated to be greater than 750 000 cases per year in the United States. Clinicians should understand the basic pharmacology of available vasoactive agents to allow for routine and complex management of septic shock. Conclusion While advances in research, identification, and early implementation of best practices for the treatment of sepsis has reduced mortality, rates remain high. Vasopressors and inotropes remain part of the core therapeutic modalities of sepsis management. Norepinephrine is the first-line vasopressor of choice for septic shock, though secondary vasopressors can be used depending on the patient’s circumstances.