Miracle-dz/UniFAR-FTUs huggingface dataset:
Factors affecting North Carolina dental hygienists' confidence in providing obesity education and counseling.

PURPOSE Obesity is a major public health issue in the United States. Dental hygienists influence their patients' oral health by providing dietary and behavioral recommendations that encourage good oral health practices. However, it is not known if they are ready to provide behavioral counseling strategies for weight loss. This study investigates whether dental hygienists in North Carolina are confident to counsel patients who are at-risk for obesity. METHODS A questionnaire was used to survey 246 dental hygienists attending a continuing education (CE) course. It investigated self-reported confidence in providing obesity counseling, educational preparation, outcome expectations and self-efficacy. The primary outcome was confidence in providing weight loss counseling. Mantel Haenszel statistics were used to compare group of interest. RESULTS Of the dental hygienists surveyed, 43% perceived an increase of overweight patients in their practices. Nearly all (95%) felt that dental hygienists have a role in helping patients improve nutrition. Over half (65%) expressed confidence in discussing obesity-related health risks. On average, the confidence in getting patients to follow weight loss advice was significantly different (p=0.02) for those with a 2 year degree and those with a 4 year degree. CONCLUSIONS The findings indicate that many North Carolina dental hygienists are willing to discuss obesity with patients.
Palaeoenvironments and palaeoceanography changes across the Jurassic/Cretaceous boundary in the Arctic realm: case study of the Nordvik section (north Siberia, Russia)

The Jurassic/Cretaceous transition was accompanied by significant changes in palaeoceanography and palaeoenvironments in the Tethyan Realm, but outside the Tethys such data are very scarce. Here we present results of a study of the most complete section in the Panboreal Superrealm, the Nordvik section. Belemnite δ18O data show an irregular decrease from values reaching up to +1.6‰ in the Middle Oxfordian and from +0.8 to −1.7‰ in the basal Ryazanian, indicating a prolonged warming. The biodiversity changes were strongly related to sea-level oscillations, showing a relatively low belemnite and high ammonite diversity during sea-level rise, accompanied by a decrease of the macrobenthos taxonomical richness. The most prominent sea-level rise is marked by the occurrence of open sea ammonites with Pacific affinities. Peak abundances of spores and prasinophytes correlate with a negative excursion in organic carbon δ13C near the J/K boundary and could reflect blooms of green algae caused by disturbance of the marine ecosystem.
Modulation of NK Cell Autocrine-Induced Eosinophil Chemotaxis by Interleukin-15 and Vitamin D3: A Possible NK-Eosinophil Crosstalk via IL-8 in the Pathophysiology of Allergic Rhinitis

Natural killer cells (NK) secrete eosinophilotactic cytokines, however, whether they contribute to eosinophil chemotaxis by secreting IL-8 is not known. We investigated the ability of CD56+CD3-ve (NK cells) to induce chemotaxis of peripheral blood eosinophils from allergic rhinitis (AR) patients, through IL-8 secretion, and the effects of IL-15, the NK cell proactivating cytokine, and calcitriol: 1α, 25-dihydroxy Vitamin D3 (vitamin D3), the immunomodulator agent, in this scenario. Herein, it is shown that supernatants from unstimulated NK cells exhibited chemotactic activity against eosinophil. This effect was significantly augmented by IL-15 (1 ng/mL) treatment, resulting in an increase in the chemotactic index of approximately 3 folds and was abrogated by neutralizing antibody (Ab) to IL-8 in a dose-dependent fashion. The amount of IL-8 secreted by NK cells was increased by IL-15 treatment from levels of 88.64 ± 21.5 to 178.9 ± 23.6 Pg/mL and was significantly reduced by 10−6 M vitamin D3 to levels of 59.2 ± 16.3 Pg/mL. Our results indicate a novel inflammatory crosstalk between NK cells and eosinophils via IL-15/IL-8 axis that can be modulated by vitamin D3.
Ontogeny of somatic angiotensin-converting enzyme.

Angiotensin-converting enzyme or kininase II (ACE-KII) plays a central role in the control of circulating and tissue levels of angiotensin II and kinins. Both peptides have been implicated in the regulation of renal function and growth during normal development. We tested the hypothesis that the developing rat kidney expresses ACE-KII mRNA transcripts and the active enzyme and evaluated whether the developmental expression of the ACE-KII gene is related to changes in circulating angiotensin II and tissue kallikrein. ACE-KII mRNA and enzymatic activity were low in the newborn kidney; peak expression occurred on days 15 and 20 of postnatal life (16-fold versus day 1). In extrarenal tissues, ACE-KII activity and mRNA levels were also low during the newborn period in the following order of abundance: lung > kidney > aorta > heart. The lung showed a higher age-related increase in active ACE-KII and mRNA abundance (15-fold) than heart and aorta (activity, 3- to 4-fold; mRNA, 6- to 10-fold). The developmental profile of ACE-KII correlated temporally with changes in circulating angiotensin II and tissue kallikrein. Plasma angiotensin II levels were 2.5-fold higher in newborn than adult rats, whereas renal and extrarenal kallikrein-like activity increased twofold to fivefold from birth to adulthood. These results demonstrate that the ACE-KII gene is developmentally regulated in a tissue-specific manner. Tissue kinin generation and degradation, reflected by kallikrein and ACE-KII activities, are coordinately regulated during development, whereas circulating angiotensin II and tissue ACE-KII change in a reciprocal manner. (ABSTRACT TRUNCATED AT 250 WORDS)
Effects of Vegetation on Turbulence, Sediment Transport, and Stream Morphology

AbstractVegetation as multiple stems in various configurations or an isolated stem profoundly alters turbulent flows. Past research has shown that these alterations influence sediment transport and stream morphology, but entail complex interactions and feedbacks between flow, vegetation, and sediment processes that involve many parameters. These interactions are examined here for a variety of macrophyte patterns and scales in riverine environments. Flow Reynolds number, canopy density, and submergence ratio are just a few of the key parameters that influence the spatial variability of the flow, momentum transfer, vortex shedding and dissipation, and instantaneous stresses that are known to affect sediment and morphological processes in rivers. Knowledge gaps, though, still remain. A taxonomy that classifies vegetated flows as dense, sparse, or isolated on the basis of threshold parameters like the ratio of stem diameter to stem spacing would be useful for comparing studies among researchers and predicting.
Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology

Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like PrnpB and variants of PrbcL, and a version of the promoter Ptrc with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.
Pure Topographical Disorientation Following a Right Forceps Major of the Splenium Lesion: A Case Study

A 72-year-old man with pure topographical disorientation following a focal hemorrhage in the right forceps major of splenium was assessed at 2 weeks and 3 months after the onset. Initially, he could identify familiar buildings and landmarks, but noted topographical disorientation, dysfunction in sense of quarters, and in visuo-spatial function. The improvement of topographical disorientation was attained in 3 months, while the inability of the sense of quarters and manipulating visuo-spatial information remained unchanged. These results suggested the heading disorientation was accompanied with impaired sense of quarters, although disabled sense of quarters continued beyond the recovery of heading disorientation.
The structural basis of proteolytic activation of bovine glutamate dehydrogenase

In this work, we re‐examine the previously reported phenomenon of the creation of a superactive glutamate dehydrogenase by proteolytic modification by chymotrypsin and explore the various discrepancies that came to light during those studies. We find that superactivation is caused by cleavage at the N terminus of the protein and not the C‐terminal allosteric site, as has previously been suggested. N‐terminal sequencing reveals that TLCK‐treated chymotrypsin cleaves bovine glutamate dehydrogenase at phenylalanine 10. We suggest that trypsin contamination in nontreated chymotrypsin may have led to the production of the larger 4–5 kDa digestion product, previously misinterpreted as having caused the activation. In line with some previous studies, we can confirm that GTP inhibition is attenuated to some extent by the proteolysis, while ADP activation is almost abolished. Utilizing the recently solved structures of bovine glutamate dehydrogenase, we illustrate the cleavage points.
Transcriptional regulation and expression of the dominant drusen gene FBLN3 (EFEMP1) in mammalian retina.

PURPOSE To determine the important transcriptional control elements and sites of expression of fibulin-3 in mammalian retina. METHODS Sequencing and 5' rapid amplification of cDNA ends (RACE) were undertaken to characterize the genomic sequence upstream of the FBLN3 coding sequence. Reporter deletion-mutation constructs were used in luciferase transfection assays to determine the important regulatory motifs. Fibulin-3 expression in mouse and human retina was studied by in situ hybridization and RT-PCR. The effect of 17beta-estradiol on fibulin-3 production was studied in COS-7 and ARPE-19 cells. RESULTS Two untranslated exons were fully sequenced completing the characterization of FBLN3 that comprises 12 exons. Reporter assays suggest that the FBLN3 proximal promoter is contained within 425 bp upstream of exon 1. Important regulatory elements include three Sp1-binding sites, a Tant motif (trans-activating) and an estrogen response element (ERE) binding site (trans-repressing). No TATA or CAAT regulatory boxes were identified. RT-PCR suggests that the fibulin-3 gene is expressed in murine and human RPE, and in situ studies confirm that Fbln3 is expressed in the outer and inner nuclear layers, but strikingly not in the ganglion cell layer. Fibulin-3 expression in ARPE-19 cells could be modified by varying the amount of estrogen in the cell culture medium. CONCLUSIONS The 5' end of the FBLN3 gene has been characterized, and the important upstream motifs regulating its transcription have been identified. Fibulin-3 is expressed in adult retina and at early stages in human and mouse development. Estrogen may be an important regulator of fibulin-3 expression, and this highlights a novel mechanism by which circulating estrogen may control the composition of the retinal extracellular matrix.
The voice prominence hypothesis: the interplay of F0 and voice source features in accentuation

This paper explores the interplay of source correlates of accentuation, examining a hypothesis (the Voice Prominence Hypothesis) that different source parameters are involved and may serve as equivalent. It predicts that where accentuation is not marked by pitch salience there will be more extensive changes in other source parameters. This follows our assumption that prosodic entities such as accentuation, focus, declination, etc. involve adjustments to the entire voice source and not simply to F0. Twelve 3-accent sentences of Connemara Irish (declaratives, WH questions and Yes/No questions) were analysed. These are typically produced and transcribed as H* H* H*L. Of particular interest were the second accents: although they are heard as accented, there are no particular pitch excursions that would account for their salience. Inverse filtering and subsequent source parameterisation was carried out to yield measures for a range of source parameters. Results support the voice prominence hypothesis: as predicted, the most striking source adjustments were found in the second accent. Even where there is substantial pitch movement (final accent), parameters other than F0 appear to be contributing to the salience of the accented syllable. The precise source changes associated with accentuation varied across sentence types and within the prosodic phrase. Index Terms: voice source, accentuation, prominence.
A substellar companion around the intermediate-mass giant star HD 11977

We report the discovery of a substellar companion to the intermediate-mass star HD 11977 (G5 III). Radial velocities of this star have been monitored for five years with FEROS at the 1.52-m ESO and later at the 2.2-m MPG/ESO telescope in of K 1 = Chile. Based on the collected data we calculated an orbital solution with a period of P = 711 days, a semi-amplitude of K 1 = 105 ms -1 , and an eccentricity of e = 0.4. The period of the radial-velocity variation is longer than that of the estimated stellar rotation, rendering it unlikely that rotational modulation is the source of the variation in the radial velocity. This hypothesis is supported by the absence of a correlation between stellar activity indicators and radial-velocity variation. By determining a primary stellar mass of M * = 1.91 M ○. , the best-fit minimum mass of the companion and semi-major axis of the orbit are m 2 sin i = 6.54 M Jup and a 2 = 1.93 AU, respectively. An upper limit for the mass of the companion of m 2 ? 65.5 M Jup has been calculated from HIPPARCOS astrometric measurements. Although the possibility of a brown-dwarf companion cannot be excluded, HD 11977 B is one of the few planet candidates detected around an intermediate-mass star. The progenitor main-sequence star of HD 11977 is probably an A-type star. This discovery gives an indirect evidence for planetary companions around early type main-sequence stars.
SPRINT: Extending RUN to Schedule Sporadic Tasks

The RUN algorithm has proven to be a very effective technique for optimal multiprocessor scheduling, thanks to the limited number of preemptions and migrations incurred by the scheduled task set. This permits to achieve high system utilisation rates typical of global scheduling approaches without paying too much penalty due to excessive preemption and migration overheads. Unfortunately, the adoption of RUN in real-world applications is limited by the missing support to sporadic task sets: we address this problem by proposing SPRINT (SPoradic Run for INdependent Tasks). SPRINT is proven correct for the vast majority of task sets and successfully scheduled all those randomly generated during our experiments. Yet, its behaviour is not defined for some specific task sets, which are however extremely rare [1]. Interestingly, experimental results show that the favourable property of causing a small number of preemptions and migrations achieved by RUN is preserved with SPRINT.
Visualization of wave‐induced suspension patterns over two‐dimensional bedforms

High spatial and temporal resolution measurements of suspended sand concentration (c) over vortex ripples were collected with a three‐transducer acoustic backscatter sensor (ABS) array, under irregular `natural' waves in a multidirectional wave basin. These measurements permit two‐dimensional visualization of the movement of sediment‐laden vortices over an individual vortex ripple under a series of waves. Patterns of sediment motion were tracked through consecutive zero‐crossings in the horizontal velocity (U) record measured at 0·05 m above the ripple crest elevation. It was possible to trace the advection of individual sediment‐laden vortices at the zero‐crossings. During 73% of these events, shedding and advection of coherent suspension events occurred before the flow reversal associated with the zero‐crossing. This may be caused by the bedforms retarding the near‐bed flow inducing the eddy shedding before the zero‐crossing. While at maxima in U, secondary suspension events with low c were observed to pass over the ripple crest moving with U measured at 0·05 m. This pattern is attributed to vortex shedding from adjacent bedforms and/or antecedent suspension events. The most energetic events appeared to persist for several wave cycles and reached heights of ≈0·20 m. These suspension events appeared to be more persistent when smaller waves follow larger waves, possibly as a result of weaker reversals in vorticity. Although the events appeared to be vertically coherent in the time series from the individual transducers, it is apparent through visualization that these events are associated with the pairing of antecedent and developing vortices.
Escape from a Dominant HLA-B*15-Restricted CD8+ T Cell Response against Hepatitis C Virus Requires Compensatory Mutations outside the Epitope

ABSTRACT Antiviral CD8+ T cells are a key component of the adaptive immune system against hepatitis C virus (HCV). For the development of immune therapies, it is essential to understand how CD8+ T cells contribute to clearance of infection and why they fail so often. A mechanism for secondary failure is mutational escape of the virus. However, some substitutions in viral epitopes are associated with fitness costs and often require compensatory mutations. We hypothesized that compensatory mutations may point toward epitopes under particularly strong selection pressure that may be beneficial for vaccine design because of a higher genetic barrier to escape. We previously identified two HLA-B*15-restricted CD8+ epitopes in NS5B (LLRHHNMVY2450-2458 and SQRQKKVTF2466-2474), based on sequence analysis of a large HCV genotype 1b outbreak. Both epitopes are targeted in about 70% of HLA-B*15-positive individuals exposed to HCV. Reproducible selection of escape mutations was confirmed in an independent multicenter cohort in the present study. Interestingly, mutations were also selected in the epitope flanking region, suggesting that compensatory evolution may play a role. Covariation analysis of sequences from the database confirmed a significant association between escape mutations inside one of the epitopes (H2454R and M2456L) and substitutions in the epitope flanking region (S2439T and K2440Q). Functional analysis with the subgenomic replicon Con1 confirmed that the primary escape mutations impaired viral replication, while fitness was restored by the additional substitutions in the epitope flanking region. We concluded that selection of escape mutations inside an HLA-B*15 epitope requires secondary substitutions in the epitope flanking region that compensate for fitness costs.
A targeted mutation at the T-cell receptor alpha/delta locus impairs T-cell development and reveals the presence of the nearby antiapoptosis gene Dad1

Locus control regions are cis gene regulatory elements comprised of DNase I-hypersensitive sites. These regions usually do not stimulate transcription outside of a chromosomal context, and therefore their ability to regulate the expression of genes is thought to occur through the modification of chromatin accessibility. A locus control region is located downstream of the T-cell receptor (TCR) alpha/delta locus on mouse chromosome 14. This locus control region is known to drive T-cell-specific TCR alpha transcription in transgenic mice. In this report, we describe a targeted deletion of this locus control region and show that this mutation acts at a critical checkpoint in alphabeta T-cell development, between the TCR-intermediate and TCR-high stages. Our analysis further reveals that the antiapoptosis gene Dad1 is at the 3' end of the TCR alpha/delta locus and that Dad1 is required for embryogenesis. We show that mouse Dad1 has a broader expression pattern than the TCR genes, in terms of both tissue and temporal specificity. Finally, we report that the chromatin between TCR alpha and Dad1 is DNase I hypersensitive in a variety of cell types, thus correlating with Dad1 expression and raising the possibility that Dad1 regulatory sequences reside in this region.
Occurrence of Deoxynivalenol and Zearalenone in Commercial Fish Feed: An Initial Study

The control of mycotoxins is a global challenge not only in human consumption but also in nutrition of farm animals including aquatic species. Fusarium toxins, such as deoxynivalenol (DON) and zearalenone (ZEN), are common contaminants of animal feed but no study reported the occurrence of both mycotoxins in fish feed so far. Here, we report for the first time the occurrence of DON and ZEN in samples of commercial fish feed designed for nutrition of cyprinids collected from central Europe. A maximal DON concentration of 825 μg kg−1 feed was found in one feed whereas average values of 289 μg kg−1 feed were noted. ZEN was the more prevalent mycotoxin but the concentrations were lower showing an average level of 67.9 μg kg−1 feed.