Miracle-dz/UniFAR-FTUs huggingface dataset:
Mortality Update of Workers Exposed to Acrylonitrile in The Netherlands

To study the possible carcinogenic effects of acrylonitrile, we updated the follow up of a cohort of 2842 acrylonitrile workers. The comparison group consisted of 3961 workers from a nitrogen fixation plant. Industrial hygiene assessments quantified past exposure to acrylonitrile, 8-hour averages as well as peak exposure, the use of personal protective equipment, and exposure to other potential carcinogenic agents. Standardized mortality ratios were calculated to adjust for the effect of age distribution, length of follow up, and temporal changes in background mortality rates. Cumulative dose-effect relations were determined for 3 exposure categories and 3 latency periods. The results show that no cancer excess seems related to exposure to acrylonitrile. This additional follow up of a cohort of 2842 workers exposed to acrylonitrile further supports the notion that occupational exposures to acrylonitrile that have occurred in the past have not noticeably increased workers’ cancer mortality rates.
Acanthoic acid and other constituents from the stem of Annona amazonica (Annonaceae)

The present work reports the isolation of acanthoic acid, a promising pimaradiene-type diterpene with several important biological activities described in the literature, from the stems of Annona amazonica. We found that acanthoic acid has significant trypanocidal activity against the epimastigote forms of Trypanosoma cruzi. This diterpene is the major constituent of the plant, comprising at least 65% of the hexane extract, demonstrating that A. amazonica is a new renewable natural source for this compound. The chemical investigation also resulted in the isolation of the alkaloids liriodenine and cassythicine, and other compounds including terpenes, sterols, and fatty acids. Additionally, the complete and unequivocal 1H and 13C NMR chemical shift assignments for cassythicine are provided.
On the precision of experimentally determined protein folding rates and ϕ‐values

ϕ‐Values, a relatively direct probe of transition‐state structure, are an important benchmark in both experimental and theoretical studies of protein folding. Recently, however, significant controversy has emerged regarding the reliability with which ϕ‐values can be determined experimentally: Because ϕ is a ratio of differences between experimental observables it is extremely sensitive to errors in those observations when the differences are small. Here we address this issue directly by performing blind, replicate measurements in three laboratories. By monitoring within‐ and between‐laboratory variability, we have determined the precision with which folding rates and ϕ‐values are measured using generally accepted laboratory practices and under conditions typical of our laboratories. We find that, unless the change in free energy associated with the probing mutation is quite large, the precision of ϕ‐values is relatively poor when determined using rates extrapolated to the absence of denaturant. In contrast, when we employ rates estimated at nonzero denaturant concentrations or assume that the slopes of the chevron arms (mf and mu) are invariant upon mutation, the precision of our estimates of ϕ is significantly improved. Nevertheless, the reproducibility we thus obtain still compares poorly with the confidence intervals typically reported in the literature. This discrepancy appears to arise due to differences in how precision is calculated, the dependence of precision on the number of data points employed in defining a chevron, and interlaboratory sources of variability that may have been largely ignored in the prior literature.
Chemical-Free Cotton Defoliation by; Mechanical, Flame and Laser Girdling

A novel new way to achieve chemical-free defoliation of cotton is discussed. The research found that by severing the phloem tissue on the main stalk, via a girdling operation, the operation stimulated the cotton plant to alter its growth into an early senescence pathway that resulted in the plant shedding its leaves and opening up all its bolls, leaving the plant in the perfect state for machine harvesting. Even with follow-up rains, zero regrowth occurred in the treated plants, unlike the untreated control plots where significant regrowth did occur. This report compares the results of greenhouse and field trials where the girdling operation was performed by hand, flame, mechanical and via a CO2 laser to achieve phloem tissue severance. Design parameters for a prototype laser girdling system are also provided. Results suggest that for deficit irrigated cotton, girdling can provide an alternative means to defoliate cotton.
Animal models of Parkinson's disease.

Animal models are important tools in experimental medical science to better understand pathogenesis of human diseases. Once developed, these models can be exploited to test therapeutic approaches for treating functional disturbances observed in the disease of interest. On the basis of experimental and clinical findings, Parkinson's disease (PD) was the first neurological disease to be modeled and, subsequently, to be treated by neurotransmitter replacement therapy. Agents that selectively disrupt or destroy catecholaminergic systems, such as reserpine, methamphetamine, 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine have been used to develop PD models. Recently, it has been found that agricultural chemicals, such as rotenone and paraquat, when administered systemically, can reproduce specific features of PD in rodents, apparently via oxidative damage. Transgenic animals that over-express alpha-synuclein are used to study the role of this protein in dopaminergic degeneration. This review critically discusses animal models of PD and compares them with characteristics of the human disease.
The Worldwide Incorporation of Women and Women’s Rights Discourse in Social Science Textbooks, 1970–2008

The invisibility of women in educational curricula and the effect this has on perpetuating women’s marginal status in society has been well documented. This article examines (1) whether and how mentions of women and women’s rights have expanded and changed in textbooks cross-nationally and over time and (2) to what extent these outcomes are driven by (a) national factors of individual countries, such as economic, political, and social development, or (b) global or transnational dynamics. We employ a quantitative analysis of the representations of women in textbooks by examining over 500 secondary school social science textbooks from 74 countries published between 1970 and 2008. Descriptive analyses reveal a steady increase in mentions of women and women’s rights in textbooks around the world. Results from multilevel models indicate the explanatory power of nation-states’ linkages to global norms of human and women’s rights in additional to national characteristics.
Is this app safe?: a large scale study on application permissions and risk signals

Third-party applications (apps) drive the attractiveness of web and mobile application platforms. Many of these platforms adopt a decentralized control strategy, relying on explicit user consent for granting permissions that the apps request. Users have to rely primarily on community ratings as the signals to identify the potentially harmful and inappropriate apps even though community ratings typically reflect opinions about perceived functionality or performance rather than about risks. With the arrival of HTML5 web apps, such user-consent permission systems will become more widespread. We study the effectiveness of user-consent permission systems through a large scale data collection of Facebook apps, Chrome extensions and Android apps. Our analysis confirms that the current forms of community ratings used in app markets today are not reliable indicators of privacy risks of an app. We find some evidence indicating attempts to mislead or entice users into granting permissions: free applications and applications with mature content request more permissions than is typical; 'look-alike' applications which have names similar to popular applications also request more permissions than is typical. We also find that across all three platforms popular applications request more permissions than average.
Modeling the acquisition front-end in high resolution gamma-ray imaging

The availability of synthetic realistic data eases design optimization, algorithm evaluation and verification of any digital system where a significant amount of digital signal processing is performed. The evolution of positron emission tomography cameras towards continuous sampling of individual position-sensitive photomultiplier anodes with processing algorithms implemented on digital programmable logic devices creates a new framework where new approaches to the /spl gamma/-event detection are possible. We have developed a system model of the acquisition chain, including multi-layer phoswich, photomultiplier, front-end analog electronics, data acquisition and digital processing. This processing includes estimation algorithms for the most relevant event parameters: energy, layer-of-interaction, time picking-off and event location. The selected simulation platform couples gently to digital hardware simulation tools, in such a way that implemented models may generate real-like stimuli for the digital system under development. The modeling of the whole front-end electronics enables deeper understanding and tuning of different system trade-offs and provides a rapid and soft transition between specification and hardware development.
Colon cancer chemopreventive effects of baicalein, an active enteric microbiome metabolite from baicalin

Baicalin is a major constituent of Scutellaria baicalensis, which is a commonly used herbal medicine in many Asian countries. After oral ingestion, intestinal micro-biota metabolism may change parent compound's structure and its biological activities. However, whether baicalin can be metabolized by enteric microbiota and the related anti-cancer activity is not clear. In this study, using human enteric microbiome incubation and HPLC analysis, we observed that baicalin can be quickly converted to baicalein. We compared the antiproliferative effects of baicalin and baicalein using a panel of human cancer cell lines, including three human colorectal cancer (CRC) cell lines. In vitro antiproliferative effects on CRC cells were verified using an in vivo xenograft nude mouse model. Baicalin showed limited antiproliferative effects on some of these cancer cell lines. Baicalein, however, showed significant antiproliferative effects in all the tested cancer cell lines, especially on HCT-116 human colorectal cancer cells. In vivo antitumor results supported our in vitro data. We demonstrated that baicalein exerts potent S phase cell cycle arrest and pro-apoptotic effects in HCT-116 cells. Baicalein induced the activation of caspase 3 and 9. The in silico modeling suggested that baicalein forms hydrogen bonds with residues Ser251 and Asp253 at the active site of caspase 3, while interactions with residues Leu227 and Asp228 in caspase 9 through its hydroxyl groups. Data from this study suggested that baicalein is a potent anticancer metabolite derived from S. baicalensis. Enteric microbiota play a key role in the colon cancer chemoprevention of S. baicalensis.
Quantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells

Cancer cells actively release extracellular vesicles (EVs), including exosomes and microvesicles, into surrounding tissues. These EVs play pleiotropic roles in cancer progression and metastasis, including invasion, angiogenesis, and immune modulation. However, the proteomic differences between primary and metastatic cancer cell-derived EVs remain unclear. Here, we conducted comparative proteomic analysis between EVs derived from human primary colorectal cancer cells (SW480) and their metastatic derivatives (SW620). Using label-free quantitation, we identified 803 and 787 proteins in SW480 EVs and SW620 EVs, respectively. Based on comparison between the estimated abundance of EV proteins, we identified 368 SW480 EV-enriched and 359 SW620 EV-enriched proteins. SW480 EV-enriched proteins played a role in cell adhesion, but SW620 EV-enriched proteins were associated with cancer progression and functioned as diagnostic indicators of metastatic cancer; they were overexpressed in metastatic colorectal cancer and played roles in multidrug resistance. As the first proteomic analysis comparing primary and metastatic cancer-derived EVs, this study increases our understanding of the pathological function of EVs in the metastatic process and provides useful biomarkers for cancer metastasis. To access the supplementary material to this article: Tables S1–S4, please see Supplementary files under Article Tools online.
Modulation of Hepatitis C Virus RNA Accumulation and Translation by DDX6 and miR-122 Are Mediated by Separate Mechanisms

DDX6 and other P-body proteins are required for efficient replication of Hepatitis C Virus (HCV) by unknown mechanisms. DDX6 has been implicated in miRNA induced gene silencing, and since efficient HCV replication and translation relies on the cellular microRNA, miR-122, we hypothesized that DDX6 had a role in the mechanism of action of miR-122. However, by using multiple HCV translation and replication assays we have found this is not the case. DDX6 silencing decreased HCV replication and translation, but did not affect the ability of miR-122 to stimulate HCV translation or promote HCV RNA accumulation. In addition, the negative effect of DDX6 silencing on HCV replication and translation was not dependent on miR-122 association with the HCV genome. Thus, DDX6 does not have a role in the activity of miR-122, and it appears that DDX6 and miR-122 modulate HCV through distinct pathways. This effect was seen in both Huh7.5 cells and in Hep3B cells, indicating that the effects are not cell type specific. Since infections by other viruses in the Flaviviridae family, including Dengue and West Nile Virus, also disrupt P-bodies and are regulated by DDX6, we speculate that DDX6 may have a common function that support the replication of several Flaviviruses.
Nature and autonomy: An organizational view of social and neurobiological aspects of self-regulation in behavior and development

The concepts of self-regulation and autonomy are examined within an organizational framework. We begin by retracing the historical origins of the organizational viewpoint in early debates within the field of biology between vitalists and reductionists, from which the construct of self-regulation emerged. We then consider human autonomy as an evolved behavioral, developmental, and experiential phenomenon that operates at both neurobiological and psychological levels and requires very specific supports within higher order social organizations. We contrast autonomy or true self-regulation with controlling regulation (a nonautonomous form of intentional behavior) in phenomenological and functional terms, and we relate the forms of regulation to the developmental processes of intrinsic motivation and internalization. Subsequently, we describe how self-regulation versus control may be characterized by distinct neurobiological underpinnings, and we speculate about some of the adaptive advantages that may underlie the evolution of autonomy. Throughout, we argue that disturbances of autonomy, which have both biological and psychological etiologies, are central to many forms of psychopathology and social alienation.
The Enculturation Experience of Three Chinese American Adolescents

The authors designed a qualitative, multiple case study that employed the photovoice method to explore how enculturation is experienced by three Chinese adolescents living with their families in a nonethnically dense cultural community. A total of 18 one-on-one interviews were conducted with three youth and their parents. Photos were also used as elicitation tools to understand the meaning of enculturation for each individual. Case descriptions of each adolescent are presented, followed by five cross-case themes: (a) Self-Identifying as Chinese, (b) Parental Strictness, (c) Multiple Groups of Comparison, (d) (Not) Having a Chinese Community, and (e) Messages to Excel. The findings provide a descriptive understanding of how adolescent enculturation is shaped by the family, community, and their intersections. Implications for research and practice, such as the continued need to understand enculturation as a dynamic phenomenon and process, are presented.
A Label-Free Electrochemical Immunosensor for Carbofuran Detection Based on a Sol-Gel Entrapped Antibody

In this study, an anti-carbofuran monoclonal antibody (Ab) was immobilized on the surface of a glassy carbon electrode (GCE) using silica sol-gel (SiSG) technology. Thus, a sensitive, label-free electrochemical immunosensor for the direct determination of carbofuran was developed. The electrochemical performance of immunoreaction of antigen with the anti-carbofuran monoclonal antibody was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), in which phosphate buffer solution containing [Fe(CN)6]3−/4− was used as the base solution for test. Because the complex formed by the immunoreaction hindered the diffusion of [Fe(CN)6]3−/4− on the electrode surface, the redox peak current of the immunosensor in the CV obviously decreased with the increase of the carbofuran concentration. The pH of working solution, the concentration of Ab and the incubation time of carbofuran were studied to ensure the sensitivity and conductivity of the immunosensor. Under the optimal conditions, the linear range of the proposed immunosensor for the determination of carbofuran was from 1 ng/mL to 100 μg/mL and from 50 μg/mL to 200 μg/mL with a detection limit of 0.33 ng/mL (S/N = 3). The proposed immunosensor exhibited good high sensitivity and stability, and it was thus suitable for trace detection of carbofuran pesticide residues.
Selective deficit of time perception in a patient with right prefrontal cortex lesion

The neural systems underlying subjective perception of time are of increasing interest for neuroscientists. Previous studies in humans and in animals have documented a role of the cerebellum and basal ganglia as an internal clock of discrete temporal units.1,2⇓ Recent data from focal lesion investigations have suggested that the frontal and the parietal lobes also are critical for time perception, especially for their role in attention and in maintaining the representation of subjective time in the working memory.3,4⇓ Neuropsychological and functional imaging studies have supported the importance of the prefrontal cortex in the perception and in the comparison of time intervals.5 We describe the case of a patient who had a selective impairment in the perception of events’ duration. A 49-year-old man reported mental confusion and difficulty with concentration. Neurologic examination showed very mild left hemiparesis that regressed in a few days. After this acute episode, …
A new flash memory management for flash storage system

Proposes a new way of managing flash memory space for flash memory-specific file systems based on a log-structured file system. Flash memory has attractive features such as non-volatility and fast I/O speed, but it also suffers from an inability to update in place, and limited usage cycles. These drawbacks require many changes to conventional storage (file) management techniques. Our focus is on lowering the cleaning cost and evenly utilizing flash memory cells while maintaining a balance between these two often-conflicting goals. The cleaning efficiency is enhanced by dynamically separating cold data and non-cold data. The second goal, cycle leveling, is achieved to the degree where the maximum difference between erase cycles is below the error range of the hardware. Simulation results show that the proposed method has a significant benefit over naive methods: a maximum of 35% reduction in the cleaning cost with evenly-spread writes across segments.
Design of a Combinatorial Bidding Market for Green Corridor Freight

The Green Corridor (GC) initiative, pursued by the Swedish government and the European Commission, is intended to be a platform for innovation for long-distance freight transport aiming for more green and efficient solutions by enhanced use of economies of scale and development of new technologies. However, scale has to be traded off by two other aspects (i) freight transport time and reliability; and (ii) economies of scope. In this paper we present an already existing rail corridor case that highlights the need for tools (and/or markets) for making more efficient tradeoffs between economies of scale and scope in long-distance freight transport. This involves making very complex coordination of shipments that are differentiated with respect to origins-destinations, shipment size, time, time reliability requirements, regularity of shipments and involving both ex ante transport planning and real-time control. A market-based method that in principle could be used to solve such complex coordination is combinatorial auctioning and we briefly review a number of Swedish public tendering cases where this is done in practice. However, this raises questions on whether a fragmented vertically separated European rail industry can mobilize the “soft” market infrastructure needed to support a Green Corridor.
A Stable and Efficient Numerical Algorithm for Unconfined Aquifer Analysis

The nonlinearity of equations governing flow in unconfined aquifers poses challenges for numerical models, particularly in field‐scale applications. Existing methods are often unstable, do not converge, or require extremely fine grids and small time steps. Standard modeling procedures such as automated model calibration and Monte Carlo uncertainty analysis typically require thousands of model runs. Stable and efficient model performance is essential to these analyses. We propose a new method that offers improvements in stability and efficiency and is relatively tolerant of coarse grids. It applies a strategy similar to that in the MODFLOW code to the solution of Richard’s equation with a grid‐dependent pressure/saturation relationship. The method imposes a contrast between horizontal and vertical permeability in gridblocks containing the water table, does not require “dry” cells to convert to inactive cells, and allows recharge to flow through relatively dry cells to the water table. We establish the accuracy of the method by comparison to an analytical solution for radial flow to a well in an unconfined aquifer with delayed yield. Using a suite of test problems, we demonstrate the efficiencies gained in speed and accuracy over two‐phase simulations, and improved stability when compared to MODFLOW. The advantages for applications to transient unconfined aquifer analysis are clearly demonstrated by our examples. We also demonstrate applicability to mixed vadose zone/saturated zone applications, including transport, and find that the method shows great promise for these types of problem as well.