Miracle-dz/UniFAR-FTUs huggingface dataset:
Exclusive localization of carbonic anhydrase in bacteriocytes of the deep-sea clam Calyptogena okutanii with thioautotrophic symbiotic bacteria

SUMMARY Deep-sea Calyptogena clams harbor thioautotrophic intracellular symbiotic bacteria in their gill epithelial cells. The symbiont fixes CO2 to synthesize organic compounds. Carbonic anhydrase (CA) from the host catalyzes the reaction CO2 + H2O ↔ HCO3− + H+, and is assumed to facilitate inorganic carbon (Ci) uptake and transport to the symbiont. However, the localization of CA in gill tissue remains unknown. We therefore analyzed mRNA sequences, proteins and CA activity in Calyptogena okutanii using expression sequence tag, SDS-PAGE and LC-MS/MS. We found that acetazolamide-sensitive soluble CA was abundantly expressed in the gill tissue of C. okutanii, and the enzyme was purified by affinity chromatography. Mouse monoclonal antibodies against the CA of C. okutanii were used in western blot analysis and immunofluorescence staining of the gill tissues of C. okutanii, which showed that CA was exclusively localized in the symbiont-harboring cells (bacteriocytes) in gill epithelial cells. Western blot analysis and measurement of activity showed that CA was abundantly (26–72% of total soluble protein) detected in the gill tissues of not only Calyptogena clams but also deep-sea Bathymodiolus mussels that harbor thioautotrophic or methanotrophic symbiotic bacteria, but was not detected in a non-symbiotic mussel, Mytilus sp. The present study showed that CA is abundant in the gill tissues of deep-sea symbiotic bivalves and specifically localizes in the cytoplasm of bacteriocytes of C. okutanii. This indicates that the Ci supply process to symbionts in the vacuole (symbiosome) in bacteriocytes is essential for symbiosis.
Aggression and substance abuse in bipolar disorder.

OBJECTIVES The goal of this retrospective study was to examine factors differentiating persons with bipolar disorder who did or did not have comorbid lifetime substance use disorders (SUD) at an index assessment. We also explored the chronology of onset of mood and SUD. METHODS We studied 146 subjects with DSM-defined bipolar disorder. Subgroups with and without lifetime SUD were compared on demographic and clinical measures. RESULTS Substance abuse disorders in this bipolar sample were associated with male sex, impulsive-aggressive traits, comorbid conduct and Cluster B personality disorders, number of suicide attempts and earlier age at onset of a first mood episode. In a multivariable logistic regression analysis, male sex and aggression and possibly earlier age at mood disorder onset were associated with SUD. In those with or without SUD, the first mood episode tended to be depressive and to precede the onset of SUD. CONCLUSIONS In persons with bipolar disorder, an earlier age of onset and aggressive traits appear to be factors associated with later development of comorbid SUD.
Exercise Blood Pressure and the Risk for Future Hypertension Among Normotensive Middle‐Aged Adults

Background The aim of the present study was to examine whether exercise blood pressure can be used to predict the development of hypertension in normotensive middle‐aged adults. Methods and Results We investigated 7082 normotensive subjects who were annually screened in a tertiary medical center and completed maximal treadmill exercise tests at each visit. After the initial 3 years, subjects were divided into approximate quartiles according to their average exercise systolic and diastolic blood pressure responses (≤158; 158 to 170; 170 to 183; ≥183 mm Hg for systolic blood pressure and ≤73; 73 to 77; 77 to 82; ≥82 mm Hg for diastolic blood pressure). Mean age of the study population was 48±9 years and 73% were men. Average baseline resting blood pressure was 120/77±12/7 mm Hg. During a follow‐up of 5±3 years, 1036 (14.6%) subjects developed hypertension. The cumulative probability of new‐onset hypertension at 5 years was significantly increased with increasing quartiles of exercise systolic blood pressure (5%, 9%, 17%, and 35%, respectively; P<0.001), with a similar association shown for diastolic blood pressure. After adjustment for baseline resting blood pressure and clinical parameters, each 5‐mm Hg increments in exercise either systolic or diastolic blood pressures were independently associated with respective 11% (P<0.001) and 30% (P<0.001) increased risk for the development of hypertension. Conclusions In normotensive middle‐aged individuals, blood pressure response to exercise is associated with future development of hypertension.
Evolution and lifetimes of flow topology in a turbulent boundary layer

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The Use of Bedside Electronic Medical Record to Improve Quality of Care in Nursing Facilities: A Qualitative Analysis

It appears that the implementation and use of a bedside electronic medical record in nursing homes can be a strategy to improve quality of care. Staff like using the bedside electronic medical record and believe it is beneficial. Information gleaned from this qualitative evaluation of four nursing homes that implemented complete electronic medical records and participated in a larger evaluation of the use of an electronic medical record will be useful to other nursing homes as they consider implementing bedside computing technology. Nursing home owners and administrators must be prepared to undertake a major change requiring many months of planning to successfully implement. Direct care staff will need support as they learn to use the equipment, especially for thefirst 6 to 12 months after implementation. There should be a careful plan for continuing education opportunities so that staff learn to properly use the software and can benefit from the technology. After 12 to 24 months, almost no one wants to return to the era of paper charting.
Biomechanical characterization of the balloon-expandable slotted tube stents

AbstrAct Purpose: The aim of the presented work was determination of the biomechanical characteristics of the vascular stent made of stainless steel (Cr-Ni-Mo) and Co-Cr-W-Ni alloy. Additionally, in order to compare obtained results, an experimental analysis of the stent made of stainless steel was carried out. Design/methodology/approach: In order to determine the strength characteristics of the analyzed stent the finite element method was applied. Geometrical model of the vascular stent, which was meshed with the use of the SOLID95 element, was worked out. Selection of the finite element was conditioned by large strains that occur during angioplastic procedure. The established boundary conditions imitated the phenomena during the balloon expansion in real conditions. Findings: The result of the analysis was determination of relationship between equivalent stresses and strains in the individual regions the stent in the function of the diameter’s change (d = 1.20 - 4.00 mm) caused by expanding pressure. Analysis of the obtained results indicates diverse distribution of stresses and strains in the stent depending on the applied biomaterial.
The formate/nitrite transporter family of anion channels

Abstract The formate/nitrite transporter (FNT) family of integral membrane proteins comprises pentameric channels for monovalent anions that exhibit a broad specificity for small anions such as chloride, the physiological cargo molecules formate, nitrite, and hydrosulfide, and also larger organic acids. Three-dimensional structures are available for the three known subtypes, FocA, NirC, and HSC, which reveal remarkable evolutionary optimizations for the respective physiological context of the channels. FNT channels share a conserved translocation pathway in each protomer, with a central hydrophobic cavity that is separated from both sides of the membrane by a narrow constriction. A single protonable residue, a histidine, plays a key role by transiently protonating the transported anion to allow an uncharged species to pass the hydrophobic barrier. Further selectivity is reached through variations in the electrostatic surface potential of the proteins, priming the formate channel FocA for anion export, whereas NirC and HSC should work bidirectionally. Electrophysiological studies have shown that a broad variety of monovalent anions can be transported, and in the case of FocA, these match exactly the products of mixed-acid fermentation, the predominant metabolic pathway for most enterobacterial species.
Providing a cloud network infrastructure on a supercomputer

Supercomputers and clouds both strive to make a large number of computing cores available for computation. More recently, similar objectives such as low-power, manageability at scale, and low cost of ownership are driving a more converged hardware and software. Challenges remain, however, of which one is that current cloud infrastructure does not yield the performance sought by many scientific applications. A source of the performance loss comes from virtualization and virtualization of the network in particular. This paper provides an introduction and analysis of a hybrid supercomputer software infrastructure, which allows direct hardware access to the communication hardware for the necessary components while providing the standard elastic cloud infrastructure for other components.
Evidence That a Peptide Spanning the B-C Junction of Proinsulin Is an Early Autoantigen Epitope in the Pathogenesis of Type 1 Diabetes1

The expression of pro(insulin) in the thymus may lead to the negative selection of pro(insulin) autoreactive T cells and peripheral tolerance to this autoantigen in type 1 diabetes (T1D). We investigated whether proinsulin is expressed in the thymus of young nonobese diabetic (NOD) mice, whether T cells from naive NOD female mice at weaning are reactive to mouse proinsulin, and the role of proinsulin as a pathogenic autoantigen in T1D. Proinsulin II mRNA transcripts were detected in the thymus of 2-wk-old NOD mice at similar levels to other control strains. Despite this expression, proinsulin autoreactive T cells were detected in the periphery of 2- to 3-wk-old naive NOD mice. Peripheral T cells reactive to the insulin, glutamic acid decarboxylase 65 (GAD65), GAD67, and islet cell Ag p69 autoantigens were also detected in these mice, indicating that NOD mice are not tolerant to any of these islet autoantigens at this young age. T cell reactivities to proinsulin and islet cell Ag p69 exceeded those to GAD67, and T cell reactivity to proinsulin in the spleen and pancreatic lymph nodes was directed mainly against a p24–33 epitope that spans the B chain/C peptide junction. Intraperitoneal immunization with proinsulin perinatally beginning at 18 days of age delayed the onset and reduced the incidence of T1D. However, s.c. immunization with proinsulin initiated at 5 wk of age accelerated diabetes in female NOD mice. Our findings support the notion that proinsulin p24–33 may be a primary autoantigen epitope in the pathogenesis of T1D in NOD mice.
VALORISATION OF STAINLESS STEEL SLAGS AS A HYDRAULIC BINDER

This work is focused on exploring various cold and hot stage treatment paths of stainless steel slag as a tool to improve its hydraulic properties. At a cold stage, mechanical and chemical activation was applied on industrial stainless steel slag; and it was found that both activation methods effectively improve the reactivity of the studied slag. In addition, the detailed investigation of hydration on two major phases, γ – C 2 S and merwinite, revealed that their hydration resulted in the formation of C – S – H gel, typically formed during the hydration of OPC. Regarding the hot stage treatment, the combination of the chemistry modification with the addition of fly ash at 30 wt. % and fast cooling by means of water quenching resulted in a complete amorphisation of the material. Ultimately, the produced material possessing similar properties to granulated blast furnace slag could be used as a latent hydraulic material in blended cements.
A quantitative approach to evaluate urinary benzene and S-phenylmercapturic acid as biomarkers of low benzene exposure

Context: Benzene is a ubiquitous pollutant; smoking habit, genetic polymorphisms, and analytical difficulties impact the identification of the best biomarker. Objective: To apply a systematic quantitative approach to evaluate urinary benzene (BEN-U) and S-phenylmercapturic acid (SPMA) as biomarkers of low benzene exposures. Methods: Seventy-one blue collar refinery workers, 97 white collar refinery workers and 108 general population subjects were included. Intrinsic characteristics, sampling and analytical issues were compared. Results: BEN-U and SPMA were detected in 99% and 78% of samples, which correlated with benzene exposure (r = 0.456 and r = 0.636, respectively) and with urinary cotinine (r = 0.630 and r = 0.570, respectively). Intrinsic characteristics were similar for the two biomarkers: specificity (0.64 and 0.69 for BEN-U and SPMA), sensitivity (0.74 and 0.83), as well as intra- and inter-individual variability (150% and >14 for both). Conclusion: BEN-U and SPMA show similar intrinsic characteristics; analytical issues in detecting SPMA suggest that BEN-U is more convenient for investigating low exposure levels.
On effective moduli of an elastic body containing periodically distributed voids: comments and corrections

For an elastic body containing periodically distributed voids, several effective techniques are presented which can be used to obtain the effective elastic moduli with any desired degree of accuracy. The results include the effects of void geometry as well as void interactions. For a body containing spherical voids, numerical results are presented and compared with those obtained by other methods.
Nitrogen Source Activates TOR (Target of Rapamycin) Complex 1 via Glutamine and Independently of Gtr/Rag Proteins*

Background: Nutrients, in particular the nitrogen source, activate TORC1 signaling in yeast. Results: The nitrogen source stimulates a rapid, transient activation of TORC1. Preferred nitrogen sources result in sustained TORC1 activity and growth via a mechanism dependent on glutamine accumulation and independent of the Gtr/Rag. Conclusion: Nutrients activate yeast TORC1 via two distinct mechanisms. Significance: Gtr/Rag is not the only mechanism to activate TORC1 in response to nutrients. The evolutionary conserved TOR complex 1 (TORC1) activates cell growth in response to nutrients. In yeast, TORC1 responds to the nitrogen source via a poorly understood mechanism. Leucine, and perhaps other amino acids, activates TORC1 via the small GTPases Gtr1 and Gtr2, orthologs of the mammalian Rag GTPases. Here we investigate the activation of TORC1 by the nitrogen source and how this might be related to TORC1 activation by Gtr/Rag. The quality of the nitrogen source, as defined by its ability to promote growth and glutamine accumulation, directly correlates with its ability to activate TORC1 as measured by Sch9 phosphorylation. Preferred nitrogen sources stimulate rapid, sustained Sch9 phosphorylation and glutamine accumulation. Inhibition of glutamine synthesis reduces TORC1 activity and growth. Poor nitrogen sources stimulate rapid but transient Sch9 phosphorylation. A Gtr1 deficiency prevents the transient stimulation of TORC1 but does not affect the sustained TORC1 activity in response to good nitrogen sources. These findings suggest that the nitrogen source must be converted to glutamine, the preferred nitrogen source in yeast, to sustain TORC1 activity. Furthermore, sustained TORC1 activity is independent of Gtr/Rag. Thus, the nitrogen source and Gtr/Rag activate TORC1 via different mechanisms.
Role of the fixed combination of fluticasone and salmeterol in adult Chinese patients with asthma and COPD

Chronic obstructive pulmonary disease (COPD) and asthma are common airway disorders characterized by chronic airway inflammation and airflow obstruction, and are a leading cause of morbidity and mortality in the People’s Republic of China. These two diseases pose a high economic burden on the family and the whole of society. Despite evidence-based Global Initiative for Chronic Obstructive Lung Disease and Global Initiative for Asthma guidelines being available for the diagnosis and management of COPD and asthma, many of these patients are not properly diagnosed or managed in the People’s Republic of China. The value of combination therapy with inhaled corticosteroids and long-acting β2-agonists has been established in the management of asthma and COPD globally. Combinations of inhaled corticosteroids and long-acting β2-agonists such as fluticasone and salmeterol, have been shown to be effective for improving symptoms, health status, and reducing exacerbations in both diseases. In this review, we discuss the efficacy and safety of this combination therapy from key studies, particularly in the People’s Republic of China.
Digital Cranial Endocast of Hyopsodus (Mammalia, “Condylarthra”): A Case of Paleogene Terrestrial Echolocation?

We here describe the endocranial cast of the Eocene archaic ungulate Hyopsodus lepidus AMNH 143783 (Bridgerian, North America) reconstructed from X-ray computed microtomography data. This represents the first complete cranial endocast known for Hyopsodontinae. The Hyopsodus endocast is compared to other known “condylarthran” endocasts, i. e. those of Pleuraspidotherium (Pleuraspidotheriidae), Arctocyon (Arctocyonidae), Meniscotherium (Meniscotheriidae), Phenacodus (Phenacodontidae), as well as to basal perissodactyls (Hyracotherium) and artiodactyls (Cebochoerus, Homacodon). Hyopsodus presents one of the highest encephalization quotients of archaic ungulates and shows an “advanced version” of the basal ungulate brain pattern, with a mosaic of archaic characters such as large olfactory bulbs, weak ventral expansion of the neopallium, and absence of neopallium fissuration, as well as more specialized ones such as the relative reduction of the cerebellum compared to cerebrum or the enlargement of the inferior colliculus. As in other archaic ungulates, Hyopsodus midbrain exposure is important, but it exhibits a dorsally protruding largely developed inferior colliculus, a feature unique among “Condylarthra”. A potential correlation between the development of the inferior colliculus in Hyopsodus and the use of terrestrial echolocation as observed in extant tenrecs and shrews is discussed. The detailed analysis of the overall morphology of the postcranial skeleton of Hyopsodus indicates a nimble, fast moving animal that likely lived in burrows. This would be compatible with terrestrial echolocation used by the animal to investigate subterranean habitat and/or to minimize predation during nocturnal exploration of the environment.
Quantitative Ultrasound at the Hand Phalanges in Adolescent Girls is Related to Their Overall Physical Fitness.

BACKGROUND Growing of the skeleton, depends on genetic, health, nutritional and environmental influences. OBJECTIVES The aim of the study was to assess the ultrasound properties of bone measured at hand phalanges in adolescent girls regarding the influence of pubertal status and level of physical fitness. MATERIAL AND METHODS The study group was 56 adolescent girls aged 12-14 yrs, the controls were 86 girls matched for age, recruited from 1256 subjects undergoing bone measurement for screening purposes. The skeletal status (amplitude-dependent speed of sound - Ad-SoS) was assessed by quantitative ultrasound (QUS) at proximal phalanges using DBM Sonic 1200. Physical fitness was assessed by the Zuchora index and pubertal development by the Tanner scale. RESULTS The adolescent girls from our study group had greater body weight, height, BMI and Ad-SoS values than the controls. Overall physical fitness was highest in girls with the lowest BMI and lowest in girls with the highest BMI values. The highest results of the Ad-SoS were reported in girls presenting highest level of physical fitness. They were statistically significantly higher than in girls with a minimal level of physical fitness. There was a statistically significant correlation between the overall physical fitness score and Ad-SoS results. No correlations between Ad-SoS and the Tanner scale, particular the elements of physical fitness (speed, jumping ability, flexibility, shoulder muscular strength, abdominal muscle strength) and BMI were shown. CONCLUSIONS The bone properties assessed by QUS at the hand phalanges in adolescent girls aged 12-14 years depend on their overall physical fitness. Pubertal development, body size and particular elements of physical fitness, especially shoulder muscular strength, have limited influence.
Calcium-Dependent Exocytosis of Atrial Natriuretic Peptide from Astrocytes

Astrocytes are non-neuronal cells in the CNS, which, like neurons, are capable of releasing neuroactive molecules. However, the mechanism of release is ill defined. In this study, we investigated the mechanism of release of atrial natriuretic peptide (ANP) from cultured cortical astrocytes by confocal microscopy. To study the discharge of this hormone, we transfected astrocytes with a construct to express pro-ANP fused with the emerald green fluorescent protein (ANP.emd). The transfection of cells with ANP.emd resulted in fluorescent puncta in the cytoplasm that represent secretory organelles. If ANP is released by exocytosis, in which the vesicle fuses with the plasma membrane, then the total intensity of the green fluorescing probe should decrease, whereas the vesicle membrane is incorporated into the plasma membrane. To monitor exocytosis, we labeled the membrane with the fluorescent styryldye FM 4-64, a reporter of cumulative exocytosis. The application of ionomycin to elevate cytoplasmic [Ca2+] increased the fluorescence intensity of FM 4-64, whereas that of ANP.emd decreased. These effects were not observed in the absence of extracellular Ca2+, suggesting that ANP is released by regulated Ca2+-dependent exocytosis from astrocytes.
Modeling, Analysis, and Optimization of Coded Caching in Small-Cell Networks

Coded caching is able to exploit accumulated cache size and hence superior to uncoded caching by distributing different fractions of a file in different nodes. This paper investigates coded caching in a large-scale small-cell network (SCN) where the locations of small base stations (SBSs) are modeled by stochastic geometry. We first propose a content delivery framework, where multiple SBSs that cache different coded packets of a desired file transmit concurrently upon a user request and the user decodes the signals using successive interference cancellation (SIC). We characterize the performance of coded caching by two performance metrics, average fractional offloaded traffic (AFOT) and average ergodic rate (AER), for which a closed-form expression and a tractable expression are derived, respectively, in the high signal-to-noise ratio region. We then formulate the coded cache placement problem for AFOT maximization as a multiple-choice knapsack problem (MCKP). By utilizing the analytical properties of AFOT, a greedy but optimal algorithm is proposed. We also consider the coded cache placement problem for AER maximization. By converting this problem into a standard MCKP, a heuristic algorithm is proposed. Analytical and numerical results reveal several design and performance insights of coded caching in conjunction with SIC receiver in interference-limited SCNs.