Miracle-dz/UniFAR-FTUs huggingface dataset:
The terminal nerve plays a prominent role in GnRH-1 neuronal migration independent from proper olfactory and vomeronasal connections to the olfactory bulbs

ABSTRACT Gonadotropin-releasing hormone-1 (GnRH-1) neurons (GnRH-1 ns) migrate from the developing olfactory pit into the hypothalamus during embryonic development. Migration of the GnRH-1 neurons is required for mammalian reproduction as these cells control release of gonadotropins from the anterior pituitary gland. Disturbances in GnRH-1 ns migration, GnRH-1 synthesis, secretion or signaling lead to varying degrees of hypogonadotropic hypogonadism (HH), which impairs pubertal onset and fertility. HH associated with congenital olfactory defects is clinically defined as Kallmann Syndrome (KS). The association of olfactory defects with HH in KS suggested a potential direct relationship between defective olfactory axonal routing, lack of olfactory bulbs (OBs) and aberrant GnRH-1 ns migration. However, it has never been experimentally proven that the formation of axonal connections of the olfactory/vomeronasal neurons to their functional targets are necessary for the migration of GnRH-1 ns to the hypothalamus. Loss-of-function of the Arx-1 homeobox gene leads to the lack of proper formation of the OBs with abnormal axonal termination of olfactory sensory neurons ( Yoshihara et al., 2005). Our data prove that correct development of the OBs and axonal connection of the olfactory/vomeronasal sensory neurons to the forebrain are not required for GnRH-1 ns migration, and suggest that the terminal nerve, which forms the GnRH-1 migratory scaffold, follows different guidance cues and differs in gene expression from olfactory/vomeronasal sensory neurons. Summary: Our work reveals that correct olfactory bulb development is not required for GnRH-1 neuronal migration. This study challenges the idea that GnRH-1 neuronal migration to the hypothalamus relies on correct routing of the olfactory and vomeronasal neurons and supports the existence of the TN in mammals.
Development of the initial diatom microfouling layer on antifouling and fouling-release surfaces in temperate and tropical Australia

Diatoms are a major component of the slime layers that form on artificial surfaces in marine environments. In this article, the role played by diatoms during the pioneering stages of colonization of three marine antifouling (AF) coatings, viz Intersmooth 360®, Super Yacht 800® and a fouling-release (FR) coating Intersleek 700®, was investigated. The study was conducted over three distinct seasons in two very different marine environments in Australia, ie temperate Williamstown, Victoria and tropical Cairns, Queensland. Diatom fouling occurred more rapidly on the FR coating Intersleek 700, compared to both biocidal AF paints. However, colonization by diatoms on all three coatings was generally slow during the 16-day study. Benthic diatoms do not subsist by floating around in the water column, rather they only gain the opportunity to colonize new surfaces when they either voluntarily release or are displaced from their benthic habitat, thereafter entering the water column where the opportunity to adhere to a new surface presents itself. However, once settled, fouling diatoms grow exponentially from the site of attachment, spreading out until they populate large areas of the surface. This mode of surface colonization correlates more with an ‘infection’ type, epidemiology model, a mechanism that accounts for the colonization of significant regions of the coating surface from a single fouling diatom cell, forming ‘clonal patches’. This is in comparison to the bacterial colonization of the surface, which exhibits far more rapid recruitment and growth of cells on the substratum surface. Therefore, it is hypothesized that fouling diatoms may be characterized more by their ability to adhere and grow on surfaces already modified by bacterial biofilms, rather than on their strength of adhesion. Cell morphology and the ability to avoid shear may also be an important factor.
Treating COPD with PDE 4 inhibitors

While the pathogenesis of chronic obstructive pulmonary disease (COPD) is incompletely understood, chronic inflammation is a major factor. In fact, the inflammatory response is abnormal, with CD8+ T-cells, CD68+ macrophages, and neutrophils predominating in the conducting airways, lung parenchyma, and pulmonary vasculature. Elevated levels of the second messenger cAMP can inhibit some inflammatory processes. Theophylline has long been used in treating asthma; it causes bronchodilation by inhibiting cyclic nucleotide phosphodiesterase (PDE), which inactivates cAMP. By inhibiting PDE, theophylline increases cAMP, inhibiting inflammation and relaxing airway smooth muscle. Rather than one PDE, there are now known to be more than 50, with differing activities, substrate preferences, and tissue distributions. Thus, the possibility exists of selectively inhibiting only the enzyme(s) in the tissue(s) of interest. PDE 4 is the primary cAMP-hydrolyzing enzyme in inflammatory and immune cells (macrophages, eosinophils, neutrophils). Inhibiting PDE 4 in these cells leads to increased cAMP levels, down-regulating the inflammatory response. Because PDE 4 is also expressed in airway smooth muscle and, in vitro, PDE 4 inhibitors relax lung smooth muscle, selective PDE 4 inhibitors are being developed for treating COPD. Clinical studies have been conducted with PDE 4 inhibitors; this review concerns those reported to date.
Heterozygous deletion at 14q22.1–q22.3 including the BMP4 gene in a patient with psychomotor retardation, congenital corneal opacity and feet polysyndactyly

Here we report on a 1‐year‐old Japanese girl with psychomotor retardation, bilateral congenital corneal opacity and bilateral postaxial polysyndactyly of the feet. Although she had a normal female karyotype, our in‐house bacterial artificial chromosome (BAC)‐based array‐CGH analysis successfully detected at least a 2.7‐Mb heterozygous deletion at 14q22.1–q22.3 harboring 18 protein‐coding genes. Among the genes, BMP4 was a candidate for the gene causing the abnormalities of both the eye and digits. It was previously reported that the BMP family was correlated with the morphogenesis of digits and ocular development, and Bmp4 heterozygous null mice revealed skeletal abnormalities including polydactyly and ocular anterior segment abnormalities. Patients with a deletion including BMP4 also hadabnormalities of the eye and digits. These previous reports support that a haplo‐insufficiency of the BMP4 gene likely caused the congenital ocular and digit abnormalities. Moreover, among the other genes contained in the deletion, GMFB is a candidate for the gene responsible for the psychomotor retardation. © 2008 Wiley‐Liss, Inc.
Embedded video in hypermedia documents: supporting integration and adaptive control

As the availability of digital video becomes commonplace, a shift in application focus will occur from merely accessing video as an independent data stream to embedding video with other multimedia data types into coordinated hypermedia presentations. The migration to embedded video will present new demands on application and support environments: processing of any one piece of video data will depend on how that data relates to other data streams active within the same presentation. This article describes presentation, synchronization, and interaction control issues for manipulating embedded video. First we describe the requirements for embedded video, contrasted against other forms of video use. Next we consider mechanisms for describing and implementing the behavior of embedded-video segments relative to other data items in a document; these relationships form the basis of implementing cooperative control among the events in a presentation. Finally we consider extending the possibilities for tailoring embedded video to the characteristics of the local runtime environment; this forms the basis for adaptive, application-level quality-of-service control of a presentation. In all cases, we describe a mechanism to externalize the behavior of hypermedia presentations containing resource-intensive data requirements so that effective control can be implemented by low-level system facilities based on application-specific requirements. We present our results in terms of the CMIFed authoring/presentation system.
Recombinant Staphylococcus Strains as Live Vectors for the Induction of Neutralizing Anti-Diphtheria Toxin Antisera

ABSTRACT We have investigated whether the nonpathogenic gram-positive bacteria Staphylococcus xylosus and S. carnosuscan display a whole domain of a toxic protein on their surface and if such vectors are suitable for immunization of BALB/c mice. The nucleotide sequence encoding the receptor-binding domain (DTR; amino acids 382 to 535) of diphtheria toxin (DT) was inserted into plasmids pSE′mp18ABPXM and pSPPmABPXM, which were designed to display heterologous proteins on S. xylosus and S. carnosus cell surfaces, respectively. Western blot analysis of the resulting bacterial lysates indicates that DTR is produced by each expression system. However, analysis of rabbit anti-DTR antisera binding to the transformed live bacteria shows that DTR is not displayed on the surface of S. xylosus cells whereas it is efficiently exposed on S. carnosus. A significant anti-DT antibody response was raised in BALB/c mice immunized intraperitoneally with S. carnosus displaying DTR, and the antisera abolished DT cytotoxicity on Vero cells. Thus, only S. carnosus can display a whole domain of a toxic protein and represents a potential vector for humoral vaccination.
Detection of Cyclospora cayetanensis Oocysts in Human Fecal Specimens by Flow Cytometry

ABSTRACT A diagnosis of cyclosporiasis typically involves stool examinations for the presence of Cyclospora oocysts by means of microscopy. In recent years, flow cytometry has been gaining in popularity as a novel method of detecting pathogens in environmental and clinical samples. The present study is an evaluation of a flow cytometric method for the detection and enumeration of Cyclospora oocysts in human fecal specimens associated with food-borne outbreaks of cyclosporiasis in Ontario, Canada. Flow cytometry results were generally very comparable to the original microscopy results for these specimens, in terms of both presence or absence of oocysts and relative oocyst concentrations. Of the 34 fecal specimens confirmed positive for Cyclospora by microscopy, 32 were also found positive by flow cytometry, and 2 others were considered equivocal. Of the eight fecal specimens reported to be negative by microscopy, two were found positive by flow cytometry and five others were considered equivocal. These two flow cytometry-positive samples and one of the equivocal samples were confirmed by microscopic reexamination, suggesting that flow cytometry may be more sensitive than microscopy. While the sample preparation time for flow cytometry is similar to or slightly longer than that for microscopy, the actual analysis time is much shorter. Further, because flow cytometry is largely automated, an analyst's levels of fatigue and expertise will not influence results. Flow cytometry appears to be a useful alternative to microscopy for the screening of large numbers of stool specimens for Cyclospora oocysts, such as in an outbreak situation.
Kinematic analysis of kicking movements in preterm infants with very low birth weight and full-term infants.

BACKGROUND AND PURPOSE Study of kicking development provides important information to understand how early spontaneous movements change in infants as they acquire voluntary control. Researchers have investigated the kicking movements of preterm infants; however, the movement patterns that they have described were inconsistent. The purpose of this study, therefore, was to examine the development of kicking movements with kinematic analysis in preterm infants with very low birth weight (VLBW) and full-term infants. SUBJECTS AND METHODS Twenty-two infants with VLBW who were divided into low gestational age (gestational age of <30 weeks, n=9) and high gestational age (gestational age of >or=30 weeks, n=13) classes and 22 full-term infants were evaluated during kicking movements using 4 synchronized cameras and 3-dimensional kinematic analysis when the infants were 2 and 4 months of corrected age. RESULTS The infants with VLBW and a high gestational age showed similar kicking movements compared with the full-term infants. In contrast, the infants with VLBW and a low gestational age exhibited a higher kick frequency and a shorter flexion phase at 4 months of corrected age. They also exhibited a higher hip-knee correlation and lower variability in the interlimb coordination pattern at 2 and 4 months of corrected age. DISCUSSION AND CONCLUSION The findings indicate that infants with VLBW, particularly those with a low gestational age, have age-related differences in movement organization and coordination of kicking compared with full-term infants.
Planning with Abstract Markov Decision Processes

Robots acting in human-scale environments must plan under uncertainty in large state–action spaces and face constantly changing reward functions as requirements and goals change. Planning under uncertainty in large state–action spaces requires hierarchical abstraction for efficient computation. We introduce a new hierarchical planning framework called Abstract Markov Decision Processes (AMDPs) that can plan in a fraction of the time needed for complex decision making in ordinary MDPs. AMDPs provide abstract states, actions, and transition dynamics in multiple layers above a base-level “flat” MDP. AMDPs decompose problems into a series of subtasks with both local reward and local transition functions used to create policies for subtasks. The resulting hierarchical planning method is independently optimal at each level of abstraction, and is recursively optimal when the local reward and transition functions are correct. We present empirical results showing significantly improved planning speed, while maintaining solution quality, in the Taxi domain and in a mobile-manipulation robotics problem. Furthermore, our approach allows specification of a decision-making model for a mobile-manipulation problem on a Turtlebot, spanning from low-level control actions operating on continuous variables all the way up through high-level object manipulation tasks.
Antimicrobial resistance of Staphylococcus aureus isolated from the intensive care unit of a general hospital in southern Brazil.

BACKGROUND Staphylococcus aureus is recognized as a major cause of nosocomial infections worldwide. The aim of this study was to evaluate the antimicrobial resistance of S. aureus isolates and the possible changes of the resistance patterns after the implementation of norms of hospital infection control. METHODOLOGY During the period of 2001 to 2004, antimicrobial resistance of S. aureus (n = 104) isolated from patients in the intensive care unit of the Hospital Divina Providencia (Porto Alegre, Brazil) was determined. The progress of S. aureus control in this hospital through 2002, after the implementation of norms for infection control, was also evaluated. RESULTS The glycopeptides presented larger in vitro activity against the isolates of S. aureus (100% of susceptibility for vancomycin and teicoplanin). The percentage of samples showing resistance to at least one drug was 96%, 97% and 100% for the years 2001, 2003 and 2004, respectively. Except for ampicillin and penicillin, antimicrobial resistance decreased from 2001 to 2004. A total of 26 phenotypic profiles were identified; among them a single profile (phenotype B) was identified in all three years of investigation, corresponding to 50% of the isolates. The number of isolated S. aureus decreased the following years, totaling 50 in 2001, 34 in 2003, and 20 in 2004. CONCLUSIONS A reduction in the number of isolates and antimicrobial resistance of S. aureus from the intensive care unit was observed after the implementation of norms for infection control.
Dynamic response of NDVI to soil moisture variations during different hydrological regimes in the Sahel region

ABSTRACT Over the last few decades, the African Sahel has become the focus of many studies regarding vegetation dynamics and their relationships with climate and people. This is because rainfall limits the production of biomass in the region, a resource on which people are directly dependent for their livelihoods. In this study, we utilized a remote-sensing approach to answering the following two questions: (1) how does the dynamic relationship between soil moisture and plant growth vary across hydrological regimes, and (2) are vegetation-type-dependent responses to soil moisture availability detectable from satellite imagery? In order to answer these questions, we studied the relationship between monthly modelled soil moisture as an indicator for water availability and the remotely sensed normalized difference vegetation index (NDVI) as a proxy for vegetation growth between a “recovery rainfall period” (1982 to 1997) and a “stable rainfall period” (1998 to 2013), at different time lags across the Sahel region. Using windowed cross-correlation, we find a strong significant positive relationship between NDVI and soil moisture at a concurrent time and at NDVI lagging behind soil moisture by 1 month for grassland, cropland, and deciduous shrubland vegetation – the dominant vegetation classes in the Sahel. South of the Sahel (the Sudanian and Guinean areas), we find longer optimal lags (soil moisture lagged by 1–3 months) in association with mixed forest and deciduous shrubland. We find no major significant change in optimal lag between the recovery and stable periods in the Sahelian region; however, in the Sudanian and Guinean areas, we observe a trend towards shorter time lags. This change in optimal lag suggests a vegetation change, which may be a response to a climatic shift or land-use change. This approach of identifying spatiotemporal trends in optimal lag correlations between modelled soil moisture and NDVI could prove to be a useful tool for mapping vegetation change and ecosystem behaviour, in turn helping inform climate change mitigation approaches and agricultural planning.
Posterior Semicircular Canal Type Benign Paroxysmal Positioning Vertigo with Ageotropic Paroxysmal Positioning Nystagmus

We report on 3 patients with typical benign paroxysmal positioning vertigo (BPPV) and atypical, paroxysmal positioning nystagmus. When the Dix-Hallpike test was performed, the patients exhibited an ageotropic nystagmus, different from that classically described in posterior semicircular canal BPPV. It was torsional-vertical with the vertical component beating downwards, and the torsional component was beating away from the lowermost ear. In both left and right Dix-Hallpike positions, the upper poles of the eyes were turning away from the lowermost ear. The atypical ageotropic paroxysmal positional nystagmus of the posterior semicircular canal was observed in the evolution of the BPPV in 2 patients and on the first examination in the third. Two patients had changing patterns of paroxysmal positioning nystagmus.
Multiplex degenerate PCR coupled with an oligo sorbent array for human endogenous retrovirus expression profiling

Human endogenous retroviruses (HERVs) can be divided into distinct families of tens to thousands of paralogous loci. The expression of HERV elements has been detected in all tissues tested to date, particularly germ cells, embryonic tissues and neoplastic tissues. Hence, the study of HERV expression could represent added value in cancer diagnosis. We developed a quantitative assay combining a multiplex degenerate PCR (MD-PCR) amplification, based on the relative conservation of the pol genes, and a colorimetric Oligo Sorbent Array (OLISA®). Nine HERV families were selected and amplification primers and capture probes were designed for each family. The features required to achieve efficient amplification of most of the elements of each HERV family and balanced co-amplification of all HERV families were analyzed. We found that MD-PCR reliability, i.e. equivalence of amplification and dose-effect relationship, relied on the adjustment of three critical parameters: the primer degeneracy, the relative concentration of each primer and the total amount of primers in the amplification mixture. The analysis of tumoral versus normal tissues suggests that this assay could prove useful in tumor phenotyping.
Virtual Velocity Approach for Estimating Bed Material Transport in Gravel‐Bed Rivers: Key Factors and Significance

In large gravel‐bed rivers, bed material transport estimation is challenging since theoretically based formulas often fail to accurately predict sediment fluxes, and it is difficult to carry out field measurements. A viable alternative to direct measurement is provided by the virtual velocity approach representing a hybrid solution to calculate the bed material transport based on a theoretical framework and use of tracers. This work aims to improve the methodology and to assess the role of input factors through a case‐study application carried out in the Parma River (Italy). Two tracer types and scour chains were deployed at four sections. Data on water level, transport processes, particle travel distances, and active layers were collected over 17 months and 6 events. The transport that occurred during two events was calculated applying different configurations taking in account for several input factors (i.e., grain size, water stage, and topography). Applying simple or more complex configurations led to significant differences in transport estimates: In relation to channel morphodynamics, different factors (e.g., variability of water level within the cross section in multithread channels) play a key role on transport processes. Results indicate that it is crucial to collect and process field data developing reach‐specific transport rating curves and to combine different type of tracers for monitoring the clast displacement lengths. Based on the methodological improvements and sensitivity analysis addressed in this study, we developed a decision tree in order to design future applications of the virtual velocity approach for estimating the bed material load in different gravel‐bed river contexts.
Social Anxiety and Posttraumatic Stress in Combat Veterans: Relations to Well-being and Character Strengths

There are few studies examining the relationship between psychopathology and positive experiences and traits. Although initial studies suggest persons with posttraumatic stress disorder (PTSD) are at increased risk for excessive social anxiety, there have been no studies to date evaluating how these conditions might interact to affect positive experiences and traits. Using self-report scales, informant ratings, and experience-sampling methodologies, we examined the association of social anxiety with well-being and character strengths in veterans with and without PTSD. Controlling for PTSD and trait negative affect, social anxiety was negatively related to global ratings of well-being and character strengths. Social anxiety also accounted for incremental variance in day-today well-being (i.e., daily affect balance, percentage of pleasant days, positive social activity, self-esteem, gratitude) over a 14-day assessment period. Although veterans with PTSD reported lower levels of global and daily well-being and character strengths than veterans without PTSD, a diagnosis of PTSD failed to exhibit unique relationships with these constructs. Building on a growing body of work, these data suggest that social anxiety is uniquely associated with disturbances in positive experiences, events, and traits. Our findings support the value of directly addressing social anxiety in the study and treatment of PTSD.