Miracle-dz/UniFAR-FTUs huggingface dataset:
Sex Steroids Affect Triglyceride Handling, Glucose-Dependent Insulinotropic Polypeptide, and Insulin Sensitivity

OBJECTIVE To evaluate metabolic effects of sex steroids in nonfasting and fasting conditions, independent from changes in body composition. RESEARCH DESIGN AND METHODS A randomized clinical trial was performed to create contrasting sex steroid levels in healthy young men: by letrozole (aromatase inhibitor) to lower estradiol (E2) and increase testosterone (group T, n = 10) versus letrozole plus E2 patches to lower T and raise E2 (group E, n = 10). Mixed meals and hyperinsulinemic-euglycemic clamps were performed before and after a 1-week treatment period. RESULTS Following intervention, the postprandial triglyceride response displayed a diverging response with a decline in group T and an increase in group E; the postprandial glucose-dependent insulinotropic polypeptide (GIP) response increased in group T. Insulin sensitivity increased in group T but remained unaltered in group E. CONCLUSIONS In healthy young men, short-term changes in sex steroids affect postprandial triglyceride and GIP response and insulin sensitivity.
Effects of 17β-estradiol (E2) on aqueous organisms and its treatment problem: a review

Abstract Natural estrogens, estrone (E1), 17β-estradiol (E2) and estriol (E3) are endocrine disrupting chemicals (EDCs) that are discharged consistently and directly into surface waters with wastewater treatment plants (WWPTs) effluents, disposal sludges and in storm-water runoff. The most common and highest potential natural estrogen that causes estrogen activity in wastewater influent is E2. This review describes and attempts to summarize the main problems involved in the removal of E2 from WWTP by traditional processes, which fundamentally rely on activated sludge and provide an insufficient treatment for E2, as well as advanced oxidation processes (AOPs) that are applied in tertiary section treatment works. Biological processes affect and play an important role in the degradation of E2. However, some investigations have reported that operations that rely on high retention times have low efficiencies. Although advanced treatment technologies are available, their cost and operational considerations do not make them sustainable solutions. Therefore, E2 is still being released into aqueous areas, as shown in this study that investigates results from different countries. E2 is present on the watch list of substances in the Water Framework Directive (WFD) of the European Union since 2013 and the minimum acceptable concentration of it is 0.4 ng/L.
The Potential of Social Identity for Equilibrium Selection

When does a common group identity improve efficiency in coordination games? To answer this question, we propose a group-contingent social preference model and derive conditions under which social identity changes equilibrium selection. We test our predictions in the minimum-effort game in the laboratory under parameter configurations which lead to an inefficient low-effort equilibrium for subjects with no group identity. For those with a salient group identity, consistent with our theory, we find that learning leads to ingroup coordination to the efficient high-effort equilibrium. Additionally, our theoretical framework reconciles findings from a number of coordination game experiments. (JEL C71, C91, D71)
Monomeric Synucleins Generate Membrane Curvature*

Background: α-Synuclein is a protein of unknown function that is critical for Parkinson disease pathology. Results: All members of the synuclein family can bend membranes when monomeric. Conclusion: Synucleins function at the presynaptic terminal to facilitate membrane curvature generation during synaptic vesicle exo- and endocytosis. Significance: Elucidating synuclein function is important for understanding vesicle trafficking and synaptic dysfunction in Parkinson disease. Synucleins are a family of presynaptic membrane binding proteins. α-Synuclein, the principal member of this family, is mutated in familial Parkinson disease. To gain insight into the molecular functions of synucleins, we performed an unbiased proteomic screen and identified synaptic protein changes in αβγ-synuclein knock-out brains. We observed increases in the levels of select membrane curvature sensing/generating proteins. One of the most prominent changes was for the N-BAR protein endophilin A1. Here we demonstrate that the levels of synucleins and endophilin A1 are reciprocally regulated and that they are functionally related. We show that all synucleins can robustly generate membrane curvature similar to endophilins. However, only monomeric but not tetrameric α-synuclein can bend membranes. Further, A30P α-synuclein, a Parkinson disease mutant that disrupts protein folding, is also deficient in this activity. This suggests that synucleins generate membrane curvature through the asymmetric insertion of their N-terminal amphipathic helix. Based on our findings, we propose to include synucleins in the class of amphipathic helix-containing proteins that sense and generate membrane curvature. These results advance our understanding of the physiological function of synucleins.
A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists.

The U.S. Environmental Protection Agency has proposed that in vitro assays for estrogen receptor (ER)- and androgen receptor (AR)-mediated actions be included in a Tier-I screening battery to detect hormonally active chemicals. Herein we describe the development of a novel stable cell line, MDA-kb2, for screening of androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine-disrupting chemicals. The breast cancer cell line, MDA-MB-453, was stably transformed with the MMTV.luciferase. neo reporter gene construct. Since both GR and AR are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either AR or GR activate the MMTV luciferase reporter. As expected, AR agonists such as dihydrotestosterone (DHT), and GR agonists such as dexamethasone (DEX), corticosterone, and aldosterone induce luciferase expression at appropriate concentrations. DHT consistently produced 3-9-fold induction at concentrations from 0.1 to 10 nM. At 1 to 1000 nM, DEX induced luciferase activity 1.3-19.5-fold. To distinguish AR- from GR-mediated ligands, chemicals were assayed concurrently with the antiandrogen, hydroxyflutamide (OHF), which blocks AR- but not GR-mediated responses. In addition, known AR antagonists, including hydroxyflutamide, vinclozolin, vinclozolin metabolites M1 and M2, p,p'-DDE, and linuron inhibited DHT-induced luciferase gene expression at appropriate concentrations in this system. We have found that these cells are relatively easy to culture and maintain. Responsiveness was monitored over time and was stable for more than 80 passages. Some advantages of this assay are that it is relatively rapid (2 days), eliminates the need for transfection, can be conducted in a 96-well plate format, and produces consistent reproducible results. In summary, we have developed a cell line that can be used to screen chemicals, not just for AR- but for GR-mediated activities as well.
Effects of coccidiosis vaccination administered by in ovo injection on Ross 708 broiler performance through 14 days of post‐hatch age

&NA; Effects of the in ovo injection of a commercial coccidiosis vaccine on various hatching chick quality variables and 14 d post‐hatch (dph) oocyst shedding have been previously examined. The current study was designed to examine the performance of Ross 708 broilers during the 14 dph period of oocyst shedding following the application of the coccidiosis vaccine. On each of 7 replicate tray levels of a single‐stage incubator, a total of 4 treatment groups was randomly represented, with each treatment group containing 63 eggs. Treatments were administered using a commercial multi‐egg injector on d 18.5 of incubation. The treatments included 3 control groups (non‐injected, dry‐punch, and diluent‐injected) and one treatment group (injected with diluent containing Inovocox EM1 vaccine). On d 21 of incubation, 20 chicks from each of the 28 treatment‐replicate groups were placed in corresponding wire‐floored battery cages. Mortality, feed intake (FI), BW gain (BWG), and feed conversion ratio (FCR) were determined for the zero to 7, 7 to 14, and cumulative zero to 14 dph intervals. There were no significant treatment effects on mortality in any interval or on BW at zero dph. There were significant treatment effects on BW at 7 and 14 dph, on BWG and FI in the zero to 7, 7 to 14, and zero to 14 dph intervals, and on FCR in the 7 to 14 and zero to 14 dph intervals. Although the performance variables of birds belonging to the diluent‐injected and vaccine‐injected groups were not significantly different, the 14 dph BW, 7 to 14 dph FI, and zero to 14 dph BWG and FI of birds belonging to the vaccine treatment group were significantly higher than those in birds belonging to the non‐injected control group. It was concluded that use of the Inovocox EM1 vaccine in commercial diluent has no detrimental effect on the overall post‐hatch performance of broilers through 14 dph.
Economic decision-making in poverty depletes cognitive control

The folk theory of the “undeserving poor” maintains that poverty is the result of bad behavior. Might poverty instead cause behavior that appears impatient or impulsive? This paper reports a randomized lab experiment and a partially randomized field experiment, both in India, and analysis of the American Time Use Survey. In all three studies, poverty is associated with lower performance and control. There is no evidence of a specific role for willpower or a generic effect of scarcity. Poverty, by making economic decision-making more difficult for the poor, appears to have depleted cognitive control. ∗dspears@princeton.edu. Princeton University. Thank you, first, to the participants, and to Vijay and Dinesh for conducting the fieldwork in Banswara. John Papp, Deepak, Pramod, and Murli helped make the experiments happen; this would have been impossible wihtout Diane Coffey. I am thankful for comments from Anne Case, Josephine Duh, Mike Geruso, Simone Schaner, and Abby Sussman. I appreciate that Sendhil Mullainathan, Eldar Shafir, and Jiaying Zhao have, at various points, discussed with me their complementary studies.
MDL, penalized likelihood, and statistical risk

We determine, for both countable and uncountable collections of functions, information-theoretic conditions on a penalty pen(f) such that the optimizer f of the penalized log likelihood criterion log 1/likelihood(f)+pen(f) has risk not more than the index of resolvability corresponding to the accuracy of the optimizer of the expected value of the criterion. If F is the linear span of a dictionary of functions, traditional description-length penalties are based on the number of non-zero terms (the lscr0 norm of the coefficients). We specialize our general conclusions to show the lscr1 norm of the coefficients times a suitable multiplier lambda is also an information-theoretically valid penalty.
Overexpression of p53, Mutation of hMLH1 and Microsatellite Instability in Gastric Carcinomas: Clinicopathologic Implications and Prognosis

Results: The expression ratios of mutated p53, inactivated hMLH1, and MSI were 32.8%, 8.4%, and 8.7%, respectively. Mutation of p53 occurred more frequently in aged group (over 40), differentiated group (against the non-differentiated group), intestinal type, infiltrative type and positive lymph node metastasis group. Inactivated hMLH1 occurred more frequently in aged group, differentiated group, intestinal type and expanding growth type group. MSI was found more frequently in aged group, intestinal type and expanding growth type group. All three genetic markers had no significant associations with the 5-year survival. Conclusion: We identified significant relationships between mutated p53, inactivated hMLH1, and MSI with some clinicopathologic features of gastric cancer. However, there were no apparent relationships between p53, hMLH1,
A nova re-accretion model for J-type carbon stars

The J-type carbon (J)-stars constitute 10–15% of the observed carbon stars in both our Galaxy and the Large Magellanic Cloud (LMC). They are characterized by strong 13 C absorption bands with low 12 C/ 13 C ratios along with other chemical signatures peculiar for typical carbon stars, e.g. a lack of s-process enhancement. Most of the J-stars are dimmer than the N-type carbon stars some of which, by hot-bottom burning, make 13 C only in a narrow range of masses. We investigate a binary-star formation channel for J-stars involving re-accretion of carbon-rich nova ejecta on main-sequence companions to low-mass carbon-oxygen white-dwarfs. The subsequent evolution of the companion stars in such systems is studied with a rapid binary evolutionary code to predict chemical signatures of nova pollution in systems which merge into giant single stars. A detailed population synthesis study is performed to estimate the number of these mergers and compare their properties with observed J-stars. Our results predict that such nova polluted mergers evolve with low luminosities as well as low 12 C/ 13 C ratios like the majority of observed J-stars (e.g. in the LMC) but cannot
Insights for conducting real-time focus groups online using a web conferencing service

Background Online focus groups have been increasing in use over the last 2 decades, including in biomedical and health-related research. However, most of this research has made use of text-based services such as email, discussion boards, and chat rooms, which do not replicate the experience of face-to-face focus groups. Web conferencing services have the potential to more closely match the face-to-face focus group experience, including important visual and aural cues. This paper provides critical reflections on using a web conferencing service to conduct online focus groups. Methods As part of a broader study, we conducted both online and face-to-face focus groups with participants. The online groups were conducted in real-time using the web conferencing service, Blackboard Collaborate TM. We used reflective practice to assess how the conduct and content of the groups were similar and how they differed across the two platforms. Results We found that further research using such services is warranted, particularly when working with hard-to-reach or geographically dispersed populations. The level of discussion and the quality of the data obtained was similar to that found in face-to-face groups. However, some issues remain, particularly in relation to managing technical issues experienced by participants and ensuring adequate recording quality to facilitate transcription and analysis. Conclusions Our experience with using web conferencing for online focus groups suggests that they have the potential to offer a realistic and comparable alternative to face-to-face focus groups, especially for geographically dispersed populations such as rural and remote health practitioners. Further testing of these services is warranted but researchers should carefully consider the service they use to minimise the impact of technical difficulties.
Methodological Challenges of Researching Positive Action Measures

This paper highlights some of the methodological challenges which have arisen in collecting data for an international study on positive action measures. It will describe strategies employed to encourage participation in the study from as wide a range of organisations and individuals using a mixed method approach. The paper will also discuss the methodological and sensitive issues related to this type of research in organisations and strategies adopted by the research team to ameliorate any problems that have arisen whilst maintaining trustworthiness and rigour in the study.
Visualizing Coronavirus RNA Synthesis in Time by Using Click Chemistry

ABSTRACT Coronaviruses induce in infected cells the formation of replicative structures, consisting of double-membrane vesicles (DMVs) and convoluted membranes, where viral RNA synthesis supposedly takes place and to which the nonstructural proteins (nsp's) localize. Double-stranded RNA (dsRNA), the presumed intermediate in RNA synthesis, is localized to the DMV interior. However, as pores connecting the DMV interior with the cytoplasm have not been detected, it is unclear whether RNA synthesis occurs at these same sites. Here, we studied coronavirus RNA synthesis by feeding cells with a uridine analogue, after which nascent RNAs were detected using click chemistry. Early in infection, nascent viral RNA and nsp's colocalized with or occurred adjacent to dsRNA foci. Late in infection, the correlation between dsRNA dots, then found dispersed throughout the cytoplasm, and nsp's and nascent RNAs was less obvious. However, foci of nascent RNAs were always found to colocalize with the nsp12-encoded RNA-dependent RNA polymerase. These results demonstrate the feasibility of detecting viral RNA synthesis by using click chemistry and indicate that dsRNA dots do not necessarily correspond with sites of active viral RNA synthesis. Rather, late in infection many DMVs may harbor dsRNA molecules that are no longer functioning as intermediates in RNA synthesis.
Survey of polycyclic aromatic hydrocarbons of vegetable oils and oilseeds by GC-MS in China

ABSTRACT There is a lack of information regarding the occurrence and content of contamination of polycyclic aromatic hydrocarbon (PAH) in edible vegetable oils and oilseeds used for oil production in China. By combining the advantages of ultrasound-assisted extraction, low temperature separation and silica SPE purification, a method for the determination of the USEPA, 16 PAHs was developed based on GC-MS to fill this gap. The method recoveries for oils and oilseeds were 84.4–113.8% and 84.3–115.3%, respectively. The LODs and LOQs for 16 PAHs were ranged from 0.06–0.17 and 0.19–0.56 μg kg–1, respectively. Based on the established method, PAH concentrations in 21 edible oils and 17 oilseeds were determined. Almost all the PAHs were found in all the samples tested, especially the light PAHs (LPAHs). Three oil samples exceeded the maximum level of 10 μg kg–1 for BaP set by China. However, five and six oil samples, respectively, exceeded the maximum limits of 2 and 10 μg kg–1 set for BaP and PAH4 by the European Union. The concentrations of PAH16 in oilseed samples were 1.5 times higher than corresponding oil samples. The relationships between PAH4 and PAH8, PAH4 and PAH16 as well as PAH8 and PAH16 indicates that PAH4 is a sufficient surrogate for the contamination level of PAHs in edible oils when compared with PAH8.
Indexing Metric Spaces with M-Tree

M-tree is a dynamic access method suitable to index generic “metric spaces”, where the function used to compute the distance between any two objects satisfies the positivity, symmetry, and triangle inequality postulates. The M-tree design fulfills typical requirements of multimedia applications, where objects are indexed using complex features, and similarity queries can require application of time-consuming distance functions. In this paper we describe the basic search and management algorithms of M-tree, introduce several heuristic split policies, and experimentally evaluate them, considering both I/O and CPU costs. Results also show that M-tree performs better than R∗-tree on highdimensional vector spaces.
Role of MICA expression, anti-MICA antibodies and serum MICA during acute rejection in a rat-to-mouse cardiac transplantation model.

BACKGROUND AND OBJECTIVE Human major histocompatibility complex class I-related gene A (MICA) is reportedly associated with poor transplant outcomes and a high risk of acute and chronic rejection in solid organ transplantation. However, studies on these risks have found conflicting results. In the present study, we investigate the MICA expression and serum MICA (sMICA) as well as the MICA antibodies (anti-MICA) in serum of recipients during acute rejection (AR) in a rat-to-mouse cardiac transplantation model. METHODS Construct rat-to-mouse concordant cardiac transplantation models, histological examination of the heart in recipients during AR at 2-6 hours time point was done. We then studied the MICA gene expression of the heart in recipients during AR at 2-6 hours time point by western blot and RT-PCR assay. We latter studied the anti-MICA and sMICA levels in serum of recipients during AR at 2-6 hours time point by Flow cytometry and ELISA measurement. RESULTS We found that Lewis rat hearts transplanted into BALB/c mice developed typical AR in 6 days. The level of severity of xenograft rejection from 2 d to 6 d was increased in a time-dependant way. MICA protein and MICA mRNA was also increased in time-dependant way and reached the highest value at 6 h. The prevalence of anti-MICA was significantly higher among those with severe acute rejection. However, sMICA was significantly increased during AR at 2 hours, then gradually decreased, and reached the lowest value at 6 h. CONCLUSIONS MICA expression in recipients' heart and anti-MICA antibodies in recipients' sera may associated with high risk of AR in rat-to-mouse transplantation. sMICA showed a negative association with acute rejection and may be a good predictor of heart transplant outcomes.
Dissemination of guidelines: which sources do physicians use in order to be informed?

OBJECTIVE To gain insight into processes of dissemination of clinical guidelines, sources of information physicians use to become informed about them, and factors influencing these processes. DESIGN National survey among a random sample of family physicians using a structured questionnaire. SETTING Family practice in The Netherlands; evaluation of a national programme of (evidence based) practice guidelines. STUDY PARTICIPANTS A random sample of 1531 family physicians. MAIN MEASURES Being informed about national guidelines and specific recommendations from these guidelines; being informed about specific educational programmes on the national guidelines; sources of information used in order to be informed; doctor and practice characteristics. RESULTS The response rate was 67%, the responders being younger than the non-responders. The scientific journal for family physicians proved to be the most important source of information (85%), discussing the guidelines in the local family doctor group was also important (53%). Following continuing medical education courses was less important (33%). On average 80% knew about the different guidelines and recommendations, 63% about the educational programmes. Doctors who use the scientific journal as a source, who are members of the professional organization of family doctors, who are younger and who are actively involved in education on family medicine, proved to be better informed. CONCLUSIONS Segmentation of the target group is necessary for effective dissemination of guidelines or new research findings. For some doctors it is desirable to make evidence available quickly, for others spreading the guidelines through the local network may be effective, while for another group a more active, personal approach may be necessary.