Miracle-dz/UniFAR-FTUs huggingface dataset:
Finite Difference , Finite element and B-Spline Collocation Methods Applied to Two Parameter Singularly Perturbed Boundary Value Problems 1

The objective of this paper is to present a comparative study of fitted-mesh finite difference method, Ritz-Galerkin finite element method and B-spline collocation method for a two-parameter singularly perturbed boundary value problems. Due to the small parameters ε and μ, the boundary layers arise. We have taken a piecewise-uniform fittedmesh to resolve the boundary layers and shown that fitted-mesh finite difference method has almost first order parameter-uniform convergence, Ritz-Galerkin finite element method has almost second order parameter-uniform convergence and B-spline collocation method has second order parameter-uniform convergence. Numerical experiments support these theoretical results. c ⃝ 2011 European Society of Computational Methods in Sciences and Engineering
Incidence of platinum‐induced ototoxicity in pediatric patients in Quebec

The antineoplastic agents cisplatin and carboplatin are widely‐used and highly‐effective against a variety of pediatric cancers. Unfortunately, ototoxicity is a frequently encountered side effect of platinum‐based chemotherapy. There is currently no treatment or prevention for platinum‐induced ototoxicity and development of hearing loss may lead to devastating consequences on the quality of life of pediatric cancer survivors. The objective of this study is to determine the incidence of platinum‐induced ototoxicity in a large series of pediatric patients and to evaluate the incidence of progression of ototoxicity after completion of treatment.
Cortical language activation in stroke patients recovering from aphasia with functional MRI.

BACKGROUND AND PURPOSE Two mechanisms for recovery from aphasia, repair of damaged language networks and activation of compensatory areas, have been proposed. In this study, we investigated whether both mechanisms or one instead of the other take place in the brain of recovered aphasic patients. METHODS Using blood oxygenation level-dependent functional MRI (fMRI), we studied cortical language networks during lexical-semantic processing tasks in 7 right-handed aphasic patients at least 5 months after the onset of left-hemisphere stroke and had regained substantial language functions since then. RESULTS We found that in the recovered aphasic patient group, functional language activity significantly increased in the right hemisphere and nonsignificantly decreased in the left hemisphere compared with that in the normal group. Bilateral language networks resulted from partial restitution of damaged functions in the left hemisphere and activation of compensated (or recruited) areas in the right hemisphere. Failure to restore any language function in the left hemisphere led to predominantly right hemispheric networks in some individuals. However, better language recovery, at least for lexical-semantic processing, was observed in individuals who had bilateral rather than right hemisphere-predominant networks. CONCLUSIONS The results indicate that the restoration of left-hemisphere language networks is associated with better recovery and inversely related to activity in the compensated or recruited areas of the right hemisphere.
Role of Central and Peripheral Adrenergic Mechanisms in Neurogenic Hypertension Produced by Brainstem Lesions in Rat

Bilateral lesions of the nucleus tractus solitarius (NTS) in rats result in acute fulminating hypertension (NTS hypertension) as a consequence of central deafferentation of baroreceptors. The hypertension is due to increased peripheral resistance and decreased cardiac output. The hypertension is blocked and cardiac output is increased by phentolamine, trimethaphan (Arfonad), and reserpine but not by propranolol. In the present experiment, systemically administered 6-hydroxydopamine (6-OH-DA) did not alter NTS hypertension if the adrenal glands were intact. Adrenalectomy, however, blocked the lesion-induced rise in blood pressure in 6-OH-DA-treated rats. Intracisternally administered 6-OH-DA (600 μg) lowered the concentration of norepinephrine only in the spinal cord and blocked the development of NTS hypertension. Local injection of 6-OH-DA into the lateral hypothalamus did not affect the hypertension. Injection of 6-OH-DA into the NTS resulted in a mild, transient elevation in blood pressure. The results of these experiments demonstrate that (1) NTS hypertension is due to increased sympathetic neural discharge, (2) during NTS hypertension sufficient adrenomedullary catecholamines are released to produce hypertension when sympathetic terminals are destroyed, (3) central noradrenergic neurons participate in the expression of NTS hypertension, and (4) baroreceptors can inhibit the release of adrenal catecholamines.
Analysis of organotins in seawater of the Southern Ocean and Suruga Bay, Japan, by gas chromatography/inductively coupled plasma mass spectrometry

Organotins are widespread in the world's oceans and have a detrimental effect on organisms. However, there is little information on their distribution in the Southern hemisphere. We analyzed organotins in seawater from the Southern Ocean and Suruga Bay, Japan, using gas chromatography/inductively coupled plasma mass spectrometry. Organotins were compared in two contrasting environments—one in shallow, temperate coastal waters (Suruga Bay) and the other in cold, deep waters of the far Southern Ocean. Twelve kinds of organotins were detected from Suruga Bay, with tributyltin (0.184–13.6 ng Sn/L) and total organotins (0.801–19.7 ng Sn/L). In contrast, three kinds of organotins were detected in the Southern Ocean, with total organotins (from not detected to 0.266 ng Sn/L). The ratios of degraded products of tributyltin and triphenyltin from the Southern Ocean were higher than those in Suruga Bay, suggesting that fresh input of organotins into the Southern Ocean is relatively low. The presence of butyltins in Antarctic sediments and biota has been demonstrated previously; however, the present study is the first to describe trace levels of organotins in the Southern Ocean approximately 1,000 km from Antarctica.
Learning by googling

The goal of giving a well-defined meaning to information is currently shared by endeavors such as the Semantic Web as well as by current trends within Knowledge Management. They all depend on the large-scale formalization of knowledge and on the availability of formal metadata about information resources. However, the question how to provide the necessary formal metadata in an effective and efficient way is still not solved to a satisfactory extent. Certainly, the most effective way to provide such metadata as well as formalized knowledge is to let humans encode them directly into the system, but this is neither efficient nor feasible. Furthermore, as current social studies show, individual knowledge is often less powerful than the collective knowledge of a certain community.As a potential way out of the knowledge acquisition bottleneck, we present a novel methodology that acquires collective knowledge from the World Wide Web using the GoogleTM API. In particular, we present PANKOW, a concrete instantiation of this methodology which is evaluated in two experiments: one with the aim of classifying novel instances with regard to an existing ontology and one with the aim of learning sub-/superconcept relations.
Microtubule-Associated Protein AtMPB2C Plays a Role in Organization of Cortical Microtubules, Stomata Patterning, and Tobamovirus Infectivity1

AtMPB2C is the Arabidopsis (Arabidopsis thaliana) homolog of MPB2C, a microtubule-associated host factor of tobacco mosaic virus movement protein that was been previously identified in Nicotiana tabacum. To analyze the endogenous function of AtMPB2C and its role in viral infections, transgenic Arabidopsis plant lines stably overexpressing green fluorescent protein (GFP)-AtMPB2C were established. The GFP-AtMPB2C fusion protein was detectable in various cell types and organs and localized at microtubules in a punctuate pattern or in filaments. To determine whether overexpression impacted on the cortical microtubular cytoskeleton, GFP-AtMPB2C-overexpressing plants were compared to known microtubular marker lines. In rapidly elongated cell types such as vein cells and root cells, GFP-AtMPB2C overexpression caused highly unordered assemblies of cortical microtubules, a disturbed, snake-like microtubular shape, and star-like crossing points of microtubules. Phenotypically, GFP-AtMPB2C transgenic plants showed retarded growth but were viable and fertile. Seedlings of GFP-AtMPB2C transgenic plants were characterized by clockwise twisted leaves, clustered stomata, and enhanced drought tolerance. GFP-AtMPB2C-overexpressing plants showed increased resistance against oilseed rape mosaic virus, a close relative of tobacco mosaic virus, but not against cucumber mosaic virus when compared to Arabidopsis wild-type plants. These results suggest that AtMPB2C is involved in the alignment of cortical microtubules, the patterning of stomata, and restricting tobamoviral infections.
The Effectiveness of Using Electronic Book (Epub 3.0) with Blended Learning Approach on Science Classroom

Integrated science subjects in junior high school are developed as subjects of integrative science intended to obtain basic competencies in science and technology and to cultivate scientific thinking critically, creatively, and independently. This study aims to determine the effectiveness of digital books (epub) as learning resources in increasing knowledge and students' skills in learning science. Blended learning combines face-toface learning with e-learning using learning resources for elektronic books (epub). The results showed that the use of emulsions (epub) with a blended learning approach was effective in improving the 5 aspects of knowledge of grade sevent students in science learning. Based on the N-Gain test, the use of emulsions (epub) with a blended learning approach can increase students' knowledge by 0.65 in the medium category. The average value of science process skills after applying the elektronic book (epub) with a blended learning approach is 65.32% with the medium category.
Retroperitoneal ectopic brain: Case report and literature review

Heterotopic neural tissue (HNT) has been identified in many sites. In our literature review, we have found only two cases of HNT located in the retroperitoneum. As far as we know, cytological features of HNT have not been described. We here report a case of retroperitoneal HNT diagnosed by fine‐needle aspiration (FNA) cytology and subsequent resection. Our patient was a male infant born at 26 weeks’ gestation. A retroperitoneal solid‐cystic lesion measuring 3.8 cm was identified by abdominal ultrasound. FNA was performed and cytology showed highly cellular smears with single cells and masses of fibrillary material. Cells were small with rounded irregular nuclei and variable cytoplasm. Multinucleated cells, cells with neuronal morphology, calcifications, and hemosiderophages could also be seen. The lesion was excised and histology showed fibrillary areas, glial cells, neurons, and ependymal cells. Synaptophysin, GFAP, and EMA were expressed in distinct areas and cell types. No recurrences were observed.
New challenges in lightning impulse flashover modeling of air gaps and insulators

Lightning overvoltages were causing problems on overhead lines well before Tesla and Edison argued over the merits of ac and dc. Many of the overvoltage protection methods for distribution lines follow the same philosophy as that used for telegraph and telephone lines. Modern guides for improving distribution-line lightning performance, such as IEEE 1410, still distinguish between unpreventable outages caused by direct flashes to phase conductors and preventable faults that are caused by flashes to objects or the ground near the line.
Intergenerational Risk Decision Making: A Practical Example

There is no such thing as intergenerational decision making, at least not yet. In fact, there is no such thing as intragenerational decision making in the context of maximizing overall social good given resource limitations, there are just decisions being made in an ad hoc fashion. Even if one assumes that there is such a thing as intragenerational decision making, no uniform standard or guidance exists to make societal decisions for the common good. Risks to society are judged unevenly within the same agency and across agencies. Decisions are made in isolation and not weighed in the societal context of what is intra or intergenerationally important. The National Academy of Public Administration (NAPA) has set forth a framework for intergenerational decision making that provides a consistent and fair basis for making tough decisions in order to address difficult issues such as the long‐term disposal of nuclear wastes. NAPA recognizes that there is an intergenerational obligation that must encompass broader questions than the narrow issue of waste disposal since resources are finite and needs are great. The fundamental principles are based on sustainability with the overarching objective that “no generation should needlessly, now or in the future, deprive its successors of the opportunity to enjoy a quality of life equivalent to its own. ” Coupled with this objective are four supporting principles of trusteeship, sustainability, chain of obligation, and precaution. The NAPA process also recognizes that no decision can be final and that a “rolling future” view is better than making decisions for “all time. ” It attempts to balance the needs of the present with those of the future in an open and transparent process that is aimed at producing a decision, not just endless analysis. The U.S. Congress and president should develop a rational standard by which to judge laws that involve intra and intergenerational issues relative to the overall societal good. Present regulations need to be evaluated relative to a uniform level of risk and benefit to assess where the limited money available can do the most good for both the present and future generations in the context of NAPA sustainability principles. It is hoped that decision makers will take a serious look at this process since it can work to resolve stakeholder stalemate.
Bayesian Inference of Allele-Specific Gene Expression Indicates Abundant Cis-Regulatory Variation in Natural Flycatcher Populations

Abstract Polymorphism in cis-regulatory sequences can lead to different levels of expression for the two alleles of a gene, providing a starting point for the evolution of gene expression. Little is known about the genome-wide abundance of genetic variation in gene regulation in natural populations but analysis of allele-specific expression (ASE) provides a means for investigating such variation. We performed RNA-seq of multiple tissues from population samples of two closely related flycatcher species and developed a Bayesian algorithm that maximizes data usage by borrowing information from the whole data set and combines several SNPs per transcript to detect ASE. Of 2,576 transcripts analyzed in collared flycatcher, ASE was detected in 185 (7.2%) and a similar frequency was seen in the pied flycatcher. Transcripts with statistically significant ASE commonly showed the major allele in >90% of the reads, reflecting that power was highest when expression was heavily biased toward one of the alleles. This would suggest that the observed frequencies of ASE likely are underestimates. The proportion of ASE transcripts varied among tissues, being lowest in testis and highest in muscle. Individuals often showed ASE of particular transcripts in more than one tissue (73.4%), consistent with a genetic basis for regulation of gene expression. The results suggest that genetic variation in regulatory sequences commonly affects gene expression in natural populations and that it provides a seedbed for phenotypic evolution via divergence in gene expression.
Tangled up in two: a burst of genome duplications at the end of the Cretaceous and the consequences for plant evolution

Genome sequencing has demonstrated that besides frequent small-scale duplications, large-scale duplication events such as whole genome duplications (WGDs) are found on many branches of the evolutionary tree of life. Especially in the plant lineage, there is evidence for recurrent WGDs, and the ancestor of all angiosperms was in fact most likely a polyploid species. The number of WGDs found in sequenced plant genomes allows us to investigate questions about the roles of WGDs that were hitherto impossible to address. An intriguing observation is that many plant WGDs seem associated with periods of increased environmental stress and/or fluctuations, a trend that is evident for both present-day polyploids and palaeopolyploids formed around the Cretaceous–Palaeogene (K–Pg) extinction at 66 Ma. Here, we revisit the WGDs in plants that mark the K–Pg boundary, and discuss some specific examples of biological innovations and/or diversifications that may be linked to these WGDs. We review evidence for the processes that could have contributed to increased polyploid establishment at the K–Pg boundary, and discuss the implications on subsequent plant evolution in the Cenozoic.
Gonadotropin-independent regulation of steroidogenesis in the fetal rat testis.

UNLABELLED The goal of the present study was to determine whether the onset of fetal Leydig cell steroidogenesis is dependent on gonadotropic stimulation. The relationships between the onset of pituitary LH synthesis and secretion, and the response of testicular steroidogenesis to LH and various putative paracrine factors were examined. We found by reverse transcription-polymerase chain reaction (RT-PCR) that the LHbeta-subunit gene expression in the fetal pituitary gland starts on embryonic day (E) 16.5. Plasma LH was very low (< 5.0 ng/L) until E19.5 and increased significantly thereafter. In contrast, the greatest increase in the testicular testosterone had already occurred between E18.5 and E19.5. Hence, fetal testicular steroidogenesis must start independent of LH stimulation. Basal testosterone production in incubations of fetal testis (E16.5-19.5) was high, 50-80% of the hCG-stimulated level. In contrast, in dispersed fetal Leydig cells, basal steroidogenesis was consistently low. This suggests the presence of paracrine factors in the intact testes that stimulate their steroidogenesis. Effects of various putative paracrine factors were thereafter tested on the fetal testis. We found for the first time that both vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP-27) markedly stimulated fetal, but not adult, Leydig cells. IN CONCLUSION 1) Pituitary LH cannot be the initial stimulus for fetal testicular steroidogenesis. 2) Some paracrine factor(s) probably turn on and maintain early fetal testicular steroidogenesis before the later onset of LH secretion, although a constitutive component in the onset of steroidogenesis is also possible. 3) VIP and PACAP-27 are likely candidates for a paracrine stimulus of the fetal testis.
Disrupting neuronal transmission: mechanism of DBS?

Applying high-frequency stimulation (HFS) to deep brain structure, known as deep brain stimulation (DBS), has now been recognized an effective therapeutic option for a wide range of neurological and psychiatric disorders. DBS targeting the basal ganglia thalamo-cortical loop, especially the internal segment of the globus pallidus (GPi), subthalamic nucleus (STN) and thalamus, has been widely employed as a successful surgical therapy for movement disorders, such as Parkinson’s disease, dystonia and tremor. However, the neurophysiological mechanism underling the action of DBS remains unclear and is still under debate: does DBS inhibit or excite local neuronal elements? In this review, we will examine this question and propose the alternative interpretation: DBS dissociates inputs and outputs, resulting in disruption of abnormal signal transmission.
Stalked Bacteria: Properties of Deoxriybonucleic Acid Bacteriophage φCbK

The Caulobacter crescentus bacteriophage φCbK was studied with respect to the physical and chemical properties of both the phage and its deoxyribonucleic acid (DNA). Electron micrographs reveal the phage to be among the largest DNA bacteriophages reported, with head dimensions of 64 by 195 nm and a flexible tail 275 nm in length. The phage is composed of 57% DNA. This DNA is double-stranded as indicated by (i) the sharp increase in extinction coefficient over a narrow range of temperature increase, (ii) an increase in density in CsCl upon thermal denaturation, and (iii) the equivalence of guanine and cytosine as well as adenine and thymine, determined by chemical analysis. φCbK DNA cosediments with Escherichia coli phage T2 DNA and has therefore been assigned an S20,w value of 63.5S. The size of the phage and its DNA and the percentage of DNA indicate that the φCbK phage head is relatively loosely packed. The properties of the DNA from bacteriophage φCbK are similar to those of host C. crescentus DNA with respect to buoyant density, thermal transition point, and guanine plus cytosine content.
Type classes in Mercury

The type systems of functional languages such as Haskell have recently become more powerful and expressive. They not only allow programmers to write code that works on values of any type (genericity), they also allow programmers to require that a particular type belongs to a given type class (constrained genericity). Such code may use any of the methods of the type class, since every type that is a member of the type class must implement those methods. This capability makes it significantly easier to express solutions to many common problems, and promotes code reuse. Incorporating type classes in a logic programming language provides some new challenges. In this paper, we explain how we have extended Mercury's type system to include support for type classes. We show that type classes integrate very nicely with Mercury's mode, determinism and uniqueness systems, and describe how our implementation works.