Miracle-dz/UniFAR-FTUs huggingface dataset:
Upcycling umbilical cords: bridging regenerative medicine with neonatology

Abstract Preterm birth is a major health concern that affects 10% of all worldwide deliveries. Many preterm infants are discharged from the hospital with morbidities that lead to an increased risk for neurodevelopmental impairment, recurrent hospitalizations, and life-long conditions. Unfortunately, the treatment of these conditions is palliative rather than curative, which calls for novel and innovative strategies. Progress in regenerative medicine has offered therapeutic options for many of these conditions. Specifically, human umbilical cord mesenchymal stem cells (MSCs) and cord blood (UCB) cells have shown promise in treating adult-onset diseases. Unlike bone-marrow and embryonic derived stem cells, umbilical cord-derived cells are easily and humanely obtained, have low immunogenicity, and offer the potential of autologous therapy. While there are several studies to uphold the efficacy of umbilical cord MSCs in adult therapies, there remains an unmet need for the investigation of its use in treating neonates. The purpose of this review is to provide a summary of current information on the potential therapeutic benefits and clinical applicability of umbilical cord MSCs and UCB cells. Promising preclinical studies have now led to a research movement that is focusing on cell-based therapies for preterm infants.
Convergence of a mixed method for a semi-stationary compressible Stokes system

We propose and analyze a finite element method for a semi– stationary Stokes system modeling compressible fluid flow subject to a Navier– slip boundary condition. The velocity (momentum) equation is approximated by a mixed finite element method using the lowest order Nedelec spaces of the first kind. The continuity equation is approximated by a standard piecewise constant upwind discontinuous Galerkin scheme. Our main result states that the numerical method converges to a weak solution. The convergence proof consists of two main steps: (i) To establish strong spatial compactness of the velocity field, which is intricate since the element spaces are only div or curl conforming. (ii) To prove that the discontinuous Galerkin approximations converge strongly, which is required in view of the nonlinear pressure function. Tools involved in the analysis include a higher integrability estimate for the discontinuous Galerkin approximations, a discrete equation for the effective viscous flux, and various renormalized formulations of the discontinuous Galerkin scheme.
Molecular markers of neuronal subpopulations in layers 4, 5, and 6 of cat primary visual cortex

Cat primary visual cortex has been used as an immunogen to produce monoclonal antibodies that detect subpopulations of neurons. When tested by immunofluorescence on tissue sections of areas 17 and 18, 2 of these antibodies, VC1.1 and VC5.1, outlined a rare subpopulation of neurons located mainly in layer 4 but also in layers 5 and 6. Double- labeling immunofluorescence experiments in area 17 revealed that all VC1.1-reactive cells were also VC5.1-reactive and 83% of VC5.1-reactive cells were VC1.1-reactive, suggesting that the antibodies were reacting with the same subpopulation of cells. Both antibodies labeled similar or identical subpopulations of cells in other areas of the cat CNS, including the superior colliculus, parts of hippocampus, cerebellar deep nuclei, and rostral spinal cord. Neither antibody labeled cell bodies in the lateral geniculate nucleus. In the retina, VC1.1 labeled cell bodies and processes of some horizontal and amacrine cells, whereas VC5.1 labeled only ganglion cell axons. In the cerebellar cortex, the most prominent labeling of VC1.1 was of Purkinje cells, whereas that of VC5.1 was of Lugaro cells. Immunoblotting analyses of cat cortical homogenates demonstrated that VC1.1 recognized a major polypeptide band of Mr 95,000–105,000 and additional bands of Mr 145,000 and Mr 170,000. VC5.1 recognized bands of Mr 97,000 and Mr 150,000. Subcellular fractionation and extraction studies showed that the VC1.1 antigens were integral membrane proteins preferentially located in a synaptosomal plasma membrane fraction. The VC5.1 antigens were preferentially located in a soluble cytoplasmic or extracellular fraction. The results indicate that antibodies VC1.1 and VC5.1 recognize unique epitopes in the cat CNS and define a previously unrecognized subpopulation of cells in cat visual cortex.
Changing Women, Unchanged Men?: Sociological Perspectives on Gender in a Post-Industrial Society

Part 1 Introduction theoretical dilemmas. Part 2 Socialization in a post-industrial society: gender and the post-industrial child gender and the post-industrial adolescent gender and young adulthood. Part 3 The received wisdom queried: stigma, deviance, bodies and identity consumption, locality and identity work and identity - the indignities of labour homelife and identity - domestic bliss? conclusions - the verdict.
A Decisional Framework of Offshoring: Integrating Insights from 25 Years of Research to Provide Direction for Future

Mirroring the growing trend for firms to support their operations by locating activities abroad, research on the practice of offshoring has increased considerably in recent years. However, despite the mounting research, understanding of the key factors influencing decision-making for offshoring remains surprisingly limited due to fragmentation. In this study, we synthesize and integrate insights from different research domains in order to develop a comprehensive decisional framework for key offshoring decisions. The integrative decisional framework is based on a systematic review of offshoring research published in the most influential management and business journals in the past 25 years. In addition to providing a snapshot of the state of research on decision-making for offshoring, this study aims to stimulate future research by identifying promising research opportunities. In particular, we propose that future research should use alternative theories to incorporate overlooked aspects of decision-making, integrate different theories to account for the interdependencies between decisions, and adopt a portfolio perspective that considers each decision as part of an overall offshoring strategy.
An Examination of Several Characteristics that Affect the Prediction of OSPL90 in Hearing Aids

Objective Investigators at the National Acoustic Laboratories have provided a theoretical derivation and e-perimental validation of a formula for setting the ma-imum output of hearing aids (Dillon & Storey, 1998; Storey, Dillon, Yeend, & Wigney, 1998). Given that measurement of discomfort levels for setting maximum output can be both time-consuming and of questionable reliability, the use of a prescriptive formula warrants consideration. In this article, an extensive data base was considered and issues of normal hearing, clinical protocol, age and gender were investigated in an effort to further determine optimal maximum output settings. Design Data were gathered from five previous investigations, for a total of 433 subjects (total ears = 710). Threshold of discomfort (TD) measures were obtained using one of two adaptations of the Ascending Method of Limits, one with category anchors and one without. Results Subjects with normal hearing had significantly lower TDs than subjects with hearing loss. A different regression line for measured TDs as a function of hearing level was noted for subjects whose hearing threshold levels fell between 20 and 60 dB HL and those with thresholds above 60 dB HL. When all effects (hearing level, method, age and gender) were considered in a single predictive model for the two threshold groups, only method and threshold were significant predictors of TD. However, for the subjects with thresholds between 20 and 60 dB HL, less than 4% of the variance in TD measures could be accounted for by those factors. For subjects with threshold above 60 dB HL, 22% of the variance in TD measures could be accounted for by those variables. Conclusions For both groups of subjects (20 to 60 dB HL and above 60 dB HL) methodology and hearing thresholds are significant predictors of discomfort levels. Age and gender are not. Given the small variance accounted for by any of these factors, measures of discomfort using standardized methodologies seem warranted.
Transplantation of Noncultured Stromal Vascular Fraction Cells of Adipose Tissue Ameliorates Osteonecrosis of the Jaw–Like Lesions in Mice

The precise pathoetiology and effective treatment strategies for bisphosphonate‐related osteonecrosis of the jaw (BRONJ) remain unknown. Transplantation of noncultured stromal vascular fraction (SVF) cells has been shown to be a useful method for regenerative medicine in place of stem cell therapy. This study investigated the effects of noncultured SVF transplantation on tooth extraction socket healing in mice. Both chemotherapeutic/bisphosphonate combination therapy for 7 weeks and tooth extraction of maxillary first molars at 3 weeks after drug administration were performed using female C57BL/6J mice. Osseous and soft tissue wound healing were validated at 4 weeks postextraction using gross wound healing and histomorphometry. Here, we created a new animal model of high‐prevalence ONJ‐like lesions that mimic human progression, because human ONJ mainly occurs in female patients taking both chemotherapeutic and bisphosphonate following tooth extraction. Moreover, mice with chemotherapeutic and bisphosphonate combination therapy for 5 weeks received SVF transplantation just after tooth extraction at 3 weeks post–drug administration. Euthanasia was performed at 2 weeks postextraction to assess the transplantation effects on wound healing using gross wound healing, histomorphometry, immunohistomorphometry, quantitative real‐time polymerase chain reaction, and microcomputed tomography. We showed that systemic transplantation of noncultured SVF cells ameliorates ONJ‐like lesions by improving both osseous and soft tissue healing of tooth extraction sockets. SVF therapy significantly increased blood vessels and the ratio of M2/M1 macrophages. In addition, SVF transplantation reduced the increases in tartrate‐resistant acid phosphatase–positive (TRAP+) mononuclear cells (MNCs) and nonattached osteoclasts from the bone surface, which were significantly detected in the connective tissue of tooth extraction sockets and bone marrow by chemotherapeutic/bisphosphonate combination therapy. Our findings suggest that transplantation of noncultured SVF cells is a suitable treatment for BRONJ. Abnormal TRAP+ MNCs and nonattached osteoclasts in systemic and local environments may contribute to the development of BRONJ. © 2017 American Society for Bone and Mineral Research.
Characterizing ncRNAs in Human Pathogenic Protists Using High-Throughput Sequencing Technology

ncRNAs are key genes in many human diseases including cancer and viral infection, as well as providing critical functions in pathogenic organisms such as fungi, bacteria, viruses, and protists. Until now the identification and characterization of ncRNAs associated with disease has been slow or inaccurate requiring many years of testing to understand complicated RNA and protein gene relationships. High-throughput sequencing now offers the opportunity to characterize miRNAs, siRNAs, small nucleolar RNAs (snoRNAs), and long ncRNAs on a genomic scale, making it faster and easier to clarify how these ncRNAs contribute to the disease state. However, this technology is still relatively new, and ncRNA discovery is not an application of high priority for streamlined bioinformatics. Here we summarize background concepts and practical approaches for ncRNA analysis using high-throughput sequencing, and how it relates to understanding human disease. As a case study, we focus on the parasitic protists Giardia lamblia and Trichomonas vaginalis, where large evolutionary distance has meant difficulties in comparing ncRNAs with those from model eukaryotes. A combination of biological, computational, and sequencing approaches has enabled easier classification of ncRNA classes such as snoRNAs, but has also aided the identification of novel classes. It is hoped that a higher level of understanding of ncRNA expression and interaction may aid in the development of less harsh treatment for protist-based diseases.
Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells

Sorafenib, a tyrosine kinase inhibitor, has been demonstrated to exert anti-tumor effects. However, the molecular mechanisms underlying its effects on bladder cancer remain unknown. Here, we evaluated the mechanisms responsible for the sorafenib-induced anti-tumor effects on 5637 and T24 bladder cancer cells. We demonstrated that sorafenib reduces cell viability, stimulates lysosome permeabilization and induces apoptosis of bladder cancer cells. These effects are dependent by the activation of cathepsin B released from lysosomes. The sorafenib-increased cathepsin B activity induced the proteolysis of Bid into tBid that stimulates the intrinsic pathway of apoptosis characterized by mitochondrial membrane depolarization, oxygen radical generation and cytochrome c release. Moreover, we found that cathepsin B enzymatic activity, induced by sorafenib, is dependent on its dephosphorylation via PTEN activation and Akt inactivation. Pretreatment with orthovanadate rescued bladder cancer cells from apoptosis. In addition, the Akt inhibitor perifosine increased the sensitivity of bladder cancer cells to sorafenib-induced cytotoxicity. Overall, our results show that apoptotic cell death induced by sorafenib in bladder cancer cells is dependent on cathepsin B activity and involved PTEN and Akt signaling pathways. The Akt inhibitor perifosine increased the cytotoxic effects of sorafenib in bladder cancer cells.
Comparative Study of Expression of Smad3 in Oral Lichen Planus and Normal Oral Mucosa

Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa which is considered by the World Health Organization (WHO) as a premalignant condition. One step in malignant development is so called epithelial mesenchymal transition (EMT), a process whereby epithelial cells acquire mesenchymal characteristics. A factor known to induce EMT is the transforming growth factor-β (TGF-β), which uses the Smad proteins as mediators for its signaling. The aim of this study was to compare the expression of Smad 3 in Oral Lichen Planus and normal oral mucosa. This descriptive analytic study was performed on 30 patients with OLP (21 women and 9 men with mean age of 45.23± 2.44 years) and 20 normal oral mucosa (14 women and 6 men with mean age of 46.95± 2.21 years). The samples were studied by immunohistochemical staining. Data were analyzed with paired T-test and Wilcoxon test by SPSS software. Expression of Smad3 in OLP samples and normal oral mucosa was different. This difference was statistically significant (P<0.001). The apparently higher expression of Smad 3 in oral lichen planus compared to normal oral mucosa might help to discuss its higher potential for malignant transition.
Linking land-use projections and forest fragmentation analysis.

An econometric model of private land-use decisions is used to project land use to 2030 for each county in the continental United States. On a national scale, forest area is projected to increase overall between 0.1 and 0.2 percent per year between now and 2030. However, forest area is projected to decrease in a majority of regions, including the key forestry regions of the South and the Pacific Northwest westside. Urban area is projected to increase by 68 million acres, and cropland, pasture, rangeland, and Conservation Reserve Program land is projected to decline in area. Regional econometric models are needed to better represent region-specific economic relationships. County-level models of forest fragmentation indices are estimated for the Western United States. The core forest model is found to perform better than the model of like adjacencies for forest land. A spatially detailed analysis of forest fragmentation in Polk County, Oregon, reveals that forests become more fragmented even though forest area increases. By linking the land-use projection and forest fragmentation models, we project increases in the average county shares of core forest in 8 of the 11 Western States. The average like adjacency measure increases in six of the states. The aggregate and spatially detailed fragmentation methods are compared by projecting the fragmentation indices to 2022 for Polk County, Oregon. Considerable differences in the results were produced with the two methods, especially in the case of the like adjacency metric.
A MultiPath Network for Object Detection

The recent COCO object detection dataset presents several new challenges for object detection. In particular, it contains objects at a broad range of scales, less prototypical images, and requires more precise localization. To address these challenges, we test three modifications to the standard Fast R-CNN object detector: (1) skip connections that give the detector access to features at multiple network layers, (2) a foveal structure to exploit object context at multiple object resolutions, and (3) an integral loss function and corresponding network adjustment that improve localization. The result of these modifications is that information can flow along multiple paths in our network, including through features from multiple network layers and from multiple object views. We refer to our modified classifier as a "MultiPath" network. We couple our MultiPath network with DeepMask object proposals, which are well suited for localization and small objects, and adapt our pipeline to predict segmentation masks in addition to bounding boxes. The combined system improves results over the baseline Fast R-CNN detector with Selective Search by 66% overall and by 4x on small objects. It placed second in both the COCO 2015 detection and segmentation challenges.
Family Relationships and Family Well-Being : A Study of the Families of Nine Year-Olds in Ireland

This study is based on the first wave of data on the child cohort (nine year-olds) in the Growing Up in Ireland (GUI) survey. It examines family relationships and their associations with parent and child well-being in the families of the nine year-olds and explores social inequalities in these aspects of family circumstances. The analysis is presented under five headings: the structure of families (a term which encompasses family structure both currently and over time and also includes family size), relationship quality between parents, the individual well-being of parents, relationship quality between parents and children, and the well-being of children.
Influence of the Magnetic Field on Oscillation Spectra in Solar

In this work, we studied oscillation parameters in faculae above magnetic knots and in the adjacent to them areas. Using SDO data we analysed oscillations in magnetic strength, Doppler velocity, and intensity signals for the lower photosphere, and in intensity for the higher levels. We found that in the magnetic field strength oscillation spectra in magnetic knots, peaks at a frequency of about 4.8mHz appear, while there are no such frequencies in the adjacent facular patches of a moderate field strength. On the contrary, Doppler velocity photospheric oscillation spectra are similar for these types of regions: in both cases, the significant peaks are in the 2.5–4.5mHz range, though the oscillations in magnetic knots are 2–3 times weaker than those at the facular periphery. At the upper photosphere, the dominant frequencies in magnetic knots are 0.5–1mHz higher than in the medium-field regions. The transition region oscillations above magnetic knots mainly concentrate in the 3–6mHz range, and those above moderate-field patches concentrate below 3mHz.
Cardiac renin and angiotensins. Uptake from plasma versus in situ synthesis.

The existence of a cardiac renin-angiotensin system, independent of the circulating renin-angiotensin system, is still controversial. We compared the tissue levels of renin-angiotensin system components in the heart with the levels in blood plasma in healthy pigs and 30 hours after nephrectomy. Angiotensin I (Ang I)-generating activity of cardiac tissue was identified as renin by its inhibition with a specific active site-directed renin inhibitor. We took precautions to prevent the ex vivo generation and breakdown of cardiac angiotensins and made appropriate corrections for any losses of intact Ang I and II during extraction and assay. Tissue levels of renin (n = 11) and Ang I (n = 7) and II (n = 7) in the left and right atria were higher than in the corresponding ventricles (P < .05). Cardiac renin and Ang I levels (expressed per gram wet weight) were similar to the plasma levels, and Ang II in cardiac tissue was higher than in plasma (P < .05). The presence of these renin-angiotensin system components in cardiac tissue therefore cannot be accounted for by trapped plasma or simple diffusion from plasma into the interstitial fluid. Angiotensinogen levels (n = 11) in cardiac tissue were 10% to 25% of the levels in plasma, which is compatible with its diffusion from plasma into the interstitium. Like angiotensin-converting enzyme, renin was enriched in a purified cardiac membrane fraction prepared from left ventricular tissue, as compared with crude homogenate, and 12 +/- 3% (mean +/- SD, n = 6) of renin in crude homogenate was found in the cardiac membrane fraction and could be solubilized with 1% Triton X-100. Tissue levels of renin and Ang I and II in the atria and ventricles were directly correlated with plasma levels (P < .05), and in both tissue and plasma the levels were undetectably low after nephrectomy. We conclude that most if not all renin in cardiac tissue originates from the kidney. Results support the contentions that in the healthy heart, angiotensin production depends on plasma-derived renin and that plasma-derived angiotensinogen in the interstitial fluid is a potential source of cardiac angiotensins. Binding of renin to cardiac membranes may be part of a mechanism by which renin is taken up from plasma.
Jointly Modeling Deep Video and Compositional Text to Bridge Vision and Language in a Unified Framework

Recently, joint video-language modeling has been attracting more and more attention. However, most existing approaches focus on exploring the language model upon on a fixed visual model. In this paper, we propose a unified framework that jointly models video and the corresponding text sentences. The framework consists of three parts: a compositional semantics language model, a deep video model and a joint embedding model. In our language model, we propose a dependency-tree structure model that embeds sentence into a continuous vector space, which preserves visually grounded meanings and word order. In the visual model, we leverage deep neural networks to capture essential semantic information from videos. In the joint embedding model, we minimize the distance of the outputs of the deep video model and compositional language model in the joint space, and update these two models jointly. Based on these three parts, our system is able to accomplish three tasks: 1) natural language generation, and 2) video retrieval and 3) language retrieval. In the experiments, the results show our approach outperforms SVM, CRF and CCA baselines in predicting Subject-Verb-Object triplet and natural sentence generation, and is better than CCA in video retrieval and language retrieval tasks.