Miracle-dz/UniFAR-FTUs huggingface dataset:
Two Time Windows of Anisomycin-Induced Amnesia for Passive Avoidance Training in the Day-Old Chick

The antibiotic anisomycin (ANI), a protein synthesis inhibitor, was used to investigate the time-related changes in protein synthesis following passive avoidance training in the day-old chick. Retention of memory for this simple learning task is known to be prevented by protein synthesis inhibitors within the first hour post-training. Here we report a second, later time window during which inhibition of protein synthesis results in amnesia following one-trial passive avoidance training. Birds were given bilateral intracranial injections of ANI (10 microliters/hemisphere of a 30 mM solution) at various times relative to training and tested 24 h later. Injections given between 0.5 h prior to 1.5 h post-training or 4-5 h post-training, but not at later or at intervening times, resulted in amnesia. These results are discussed in the context of earlier findings, using the inhibitor of glycoprotein synthesis 2-deoxygalactose, that memory formation shows two glycoprotein-synthesis-dependent periods of sensitivity (Scholey, Rose, Zamani, Bock, & Schachner, 1993). The time windows of susceptibility of ANI and 2-Dgal are consistent with a model in which there are two waves of neural activity following training; during the second, commencing 4 h after training, proteins are synthesized and then glycosylated as part of the establishment of an enduring memory trace.
Literacy and religion

This book explores the interrelation of literacy and religion as practiced by Western Christians in, first, historical contexts and, second, in one contemporary church setting. Using both a case study and a Foucauldian theoretical framework, the book provides a sustained analysis of the reciprocal discursive construction of literacy, religiosity and identity in one Seventh-day Adventist Church community of Northern Australia. Critical linguistic and discourse analytic theory is used to disclose processes of theological (church), familial (home) and educational (school) normalisation of community members into regulated ways of hearing and speaking, reading and writing, being and believing. Detailed analyses of spoken and written texts taken from institutional and local community settings show how textual religion is an exemplary technology of the self, a politics constituted by canonical texts, interpretive norms, textual practices, ritualised events and sociopolitical protocols that, ultimately, are turned in upon the self. The purpose of these analyses is to show how, across denominational difference in belief (tradition) and practice, particular versions of self and society are constructed through economies of truth from text, enabling and constraining what can and cannot be spoken and enacted by believers.
Collective Stability in Structured Prediction: Generalization from One Example

Structured predictors enable joint inference over multiple interdependent output variables. These models are often trained on a small number of examples with large internal structure. Existing distribution-free generalization bounds do not guarantee generalization in this setting, though this contradicts a large body of empirical evidence from computer vision, natural language processing, social networks and other fields. In this paper, we identify a set of natural conditions-- weak dependence, hypothesis complexity and a new measure, collective stability--that are sufficient for generalization from even a single example, without imposing an explicit generative model of the data. We then demonstrate that the complexity and stability conditions are satisfied by a broad class of models, including marginal inference in templated graphical models. We thus obtain uniform convergence rates that can decrease significantly faster than previous bounds, particularly when each structured example is sufficiently large and the number of training examples is constant, even one.
A New Switched-Capacitor Boost-Multilevel Inverter Using Partial Charging

In this brief, a new switched-capacitor-boost-multilevel (SCBM) inverter is proposed and implemented. This inverter possesses the distinct features of both voltage boost up and near-sinusoidal staircase output voltage. The key is to utilize partial charging in such a way that multiple voltage steps per capacitor can be realized, hence significantly reducing the number of capacitors for a given number of levels. Based on using only two capacitors, a 13-level SCBM inverter is designed and analyzed, with emphasis on assessing its total harmonic distortion. Both simulation and experimental results are given to confirm the theoretical analysis.
Context-Based Adaptation of Mobile Applications in Tourism

Mobile guides (based on PDAs, smart phones, or mobile phones) play an increasingly important role in tourism, giving tourists ubiquitous access to relevant information especially during their trip. Due to a more difficult access to mobile applications in a ubiquitous usage environment, based on time constraints, lighting conditions, bandwidth, etc., user acceptance of mobile applications strongly depends on the application adaptation to the concrete usage context. This article presents a framework for mobile applications in tourism, enabling a flexible implementation of adaptive, context-aware tourism applications. The framework especially provides approaches for user interface adaptation, content adaptation (recommendation), and interaction modality adaptation. The framework has been prototypically instantiated and evaluated in two different application scenarios, a city guide for the city of Innsbruck and a skiing guide for the ski resort DolomitiSuperski. Both application scenarios showed high usage rates and customer satisfaction and proved the applicability and effectiveness of the presented approach for developing adaptive mobile tourism applications.
Dicer1 Is Required for Differentiation of the Mouse Male Germline1

Abstract MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression. Hundreds of miRNAs are expressed in mammals; however, their functions are just starting to be uncovered. MicroRNAs are processed from a long hairpin mRNA transcript, down to a ∼23-nucleotide duplex. The enzyme Dicer1 is required for miRNA processing, and mouse knockouts of Dicer1 are embryonic lethal before 7.5 days postcoitus. To examine the function of miRNAs specifically in the germline, we used a mouse model that expresses Cre recombinase from the TNAP locus and a floxed Dicer1 conditional allele. Removal of Dicer1 from germ cells resulted in male infertility. Germ cells were present in adult testes, but few tubules contained elongating spermatids. Germ cells that did differentiate to elongating spermatids exhibited abnormal morphology and motility. Rarely, sperm lacking Dicer1 could fertilize wild-type eggs to generate viable offspring. These results show that Dicer1 and miRNAs are essential for proper differentiation of the male germline.
Expression of RUNX 1 Correlates with Poor Patient Prognosis in Triple Negative Breast Cancer

The RUNX1 transcription factor is widely recognised for its tumour suppressor effects in leukaemia. Recently a putative link to breast cancer has started to emerge, however the function of RUNX1 in breast cancer is still unknown. To investigate if RUNX1 expression was important to clinical outcome in primary breast tumours a tissue microarray (TMA) containing biopsies from 483 patients with primary operable invasive ductal breast cancer was stained by immunohistochemistry. RUNX1 was associated with progesterone receptor (PR)-positive tumours (P,0.05), more tumour CD4+(P,0.05) and CD8+ (P,0.01) T-lymphocytic infiltrate, increased tumour CD138+plasma cell (P,0.01) and more CD68+macrophage infiltrate (P, 0.001). RUNX1 expression did not influence outcome of oestrogen receptor (ER)-positive or HER2-positive disease, however on univariate analysis a high RUNX1 protein was significantly associated with poorer cancer-specific survival in patients with ER-negative (P,0.05) and with triple negative (TN) invasive breast cancer (P,0.05). Furthermore, multivariate Cox regression analysis of cancer-specific survival showed a trend towards significance in ER-negative patients (P,0.1) and was significant in triple negative patients (P,0.05). Of relevance, triple negative breast cancer currently lacks good biomarkers and patients with this subtype do not benefit from the option of targeted therapy unlike patients with ER-positive or HER2positive disease. Using multivariate analysis RUNX1 was identified as an independent prognostic marker in the triple negative subgroup. Overall, our study identifies RUNX1 as a new prognostic indicator correlating with poor prognosis specifically in the triple negative subtype of human breast cancer. Citation: Ferrari N, Mohammed ZMA, Nixon C, Mason SM, Mallon E, et al. (2014) Expression of RUNX1 Correlates with Poor Patient Prognosis in Triple Negative Breast Cancer. PLOS ONE 9(6): e100759. doi:10.1371/journal.pone. 0100759 Editor: Arto Mannermaa, University of Eastern Finland, Finland Received February 24, 2014; Accepted May 28, 2014; Published June 26, 2014 Copyright: 2014 Ferrari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was funded by Cancer Research UK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * Email: K.Blyth@beatson.gla. ac.uk ¤ Current address: University Department of Pathology, Omar Almukhtar University, Al bayda, Libya
Short-term effects of CO2 leakage on the soil bacterial community in a simulated gas leakage scenario

The technology of carbon dioxide (CO2) capture and storage (CCS) has provided a new option for mitigating global anthropogenic emissions with unique advantages. However, the potential risk of gas leakage from CO2 sequestration and utilization processes has attracted considerable attention. Moreover, leakage might threaten soil ecosystems and thus cannot be ignored. In this study, a simulation experiment of leakage from CO2 geological storage was designed to investigate the short-term effects of different CO2 leakage concentration (from 400 g m−2 day−1 to 2,000 g m−2 day−1) on soil bacterial communities. A shunt device and adjustable flow meter were used to control the amount of CO2 injected into the soil. Comparisons were made between soil physicochemical properties, soil enzyme activities, and microbial community diversity before and after injecting different CO2 concentrations. Increasing CO2 concentration decreased the soil pH, and the largest variation ranged from 8.15 to 7.29 (p < 0.05). Nitrate nitrogen content varied from 1.01 to 4.03 mg/Kg, while Olsen-phosphorus and total phosphorus demonstrated less regular downtrends. The fluorescein diacetate (FDA) hydrolytic enzyme activity was inhibited by the increasing CO2 flux, with the average content varying from 22.69 to 11.25 mg/(Kg h) (p < 0.05). However, the increasing activity amplitude of the polyphenol oxidase enzyme approached 230%, while the urease activity presented a similar rising trend. Alpha diversity results showed that the Shannon index decreased from 7.66 ± 0.13 to 5.23 ± 0.35 as the soil CO2 concentration increased. The dominant phylum in the soil samples was Proteobacteria, whose proportion rose rapidly from 28.85% to 67.93%. In addition, the proportion of Acidobacteria decreased from 19.64% to 9.29% (p < 0.01). Moreover, the abundances of genera Methylophilus, Methylobacillus, and Methylovorus increased, while GP4, GP6 and GP7 decreased. Canonical correlation analysis results suggested that there was a correlation between the abundance variation of Proteobacteria, Acidobacteria, and the increasing nitrate nitrogen, urease and polyphenol oxidase enzyme activities, as well as the decreasing FDA hydrolytic enzyme activity, Olsen-phosphorus and total phosphorus contents. These results might be useful for evaluating the risk of potential CO2 leakages on soil ecosystems.
Genetics of Hepatobiliary Carcinogenesis

Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC) are two leading causes of cancer death in the world. Liver carcinogenesis is driven by genetic alterations in combination with viral and environmental factors. β-catenin and P53 mutations represent the two main genetic alterations described in HCC, and P53 and KRAS mutations in CC, but rare genetic alterations could be particularly valuable if they constitute drug-able targets (such as PIK3CA or EGFR mutations). Recent progress using global genomic analysis has highlighted the marked genetic heterogeneity of this disease and this approach has also been used to assess prognosis or refine the diagnosis. The validation of sorafenib as the first targeted therapy useful in HCC has opened up new prospects for biotherapy in this cancer. In the future, mapping of genetic alterations will be essential to adapt treatment to HCC and CC biology.
Enumeration of Marine Viruses in Culture and Natural Samples by Flow Cytometry

ABSTRACT Flow cytometry (FCM) was successfully used to enumerate viruses in seawater after staining with the nucleic acid-specific dye SYBR Green-I. The technique was first optimized by using thePhaeocystis lytic virus PpV-01. Then it was used to analyze natural samples from different oceanic locations. Virus samples were fixed with 0.5% glutaraldehyde and deep frozen for delayed analysis. The samples were then diluted in Tris-EDTA buffer and analyzed in the presence of SYBR Green-I. A duplicate sample was heated at 80°C in the presence of detergent before analysis. Virus counts obtained by FCM were highly correlated to, although slightly higher than, those obtained by epifluorescence microscopy or by transmission electron microscopy (r = 0.937, n = 14, andr = 0.96, n = 8, respectively). Analysis of a depth profile from the Mediterranean Sea revealed that the abundance of viruses displayed the same vertical trend as that of planktonic cells. FCM permits us to distinguish between at least two and sometimes three virus populations in natural samples. Because of its speed and accuracy, FCM should prove very useful for studies of virus infection in cultures and should allow us to better understand the structure and dynamics of virus populations in natural waters.
On the star formation rate – brightest cluster relation: estimating the peak star formation rate in post-merger galaxies

We further the recent discussion on the relation between the star formation rate (SFR) of a galaxy and the luminosity of its brightest star cluster (SFR versus M V brightest ). We first show that the observed trend of SFR versus M V brightest is due to the brightest cluster in a galaxy being preferentially young (≤15 Myr - for a constant SFR) and hence a good tracer of the current SFR, although we give notable exceptions to this rule. Archival Hubble Space Telescope (HST) imaging of high-SFR galaxies, as well as additional galaxies/clusters from the literature, is used to further confirm the observed trend. Using a series of Monte Carlo simulations, we show that a pure power-law mass function with index a = 2 is ruled out by the current data. Instead, we find that a Schechter function (i.e. a power law with an exponential truncation at the high-mass end) provides an excellent fit to the data. Additionally, these simulations show that bound cluster formation (in M ⊙ yr -1 ) represents only ∼8±3 per cent of the total star formation within a galaxy, independent of the SFR. From this, we conclude that there is only a single mode of cluster formation which operates over at least 6 orders of magnitude in the SFR. We provide a simple model of star/cluster formation feedback within dwarf galaxies (and star-forming complexes within spirals) which highlights the strong impact that a massive cluster can have on its surroundings. Using this relation, we can extrapolate backwards in time in order to estimate the peak SFR of major merger galaxies, such as NGC 7252,1316 and 3610. The derived SFRs for these galaxies are between a few hundred and a few thousand solar masses per year. The inferred far-infrared luminosity of the galaxies, from the extrapolated SFR, places them well within the range of ultraluminous infrared galaxies (ULIRGs) and for NGC 7252 within the hyperluminous infrared galaxy (HLIRG) regime. Thus, we provide evidence that these post-merger galaxies passed through a ULIRG/HLIRG phase and are now evolving passively. Using the current and extrapolated past SFR of NGC 34, we infer that the ULIRG phase of this galaxy has lasted for at least 150 Myr.
Renal function and structure in a rat model of arterial calcification and increased pulse pressure.

Clinical studies suggest a strong link between tissue calcification and pressure hyperpulsatility in end stage renal disease patients. Using a Wistar rat model of arterial elastocalcinosis and hyperpulsatility [vitamin D and nicotine (VDN) treatment], we evaluated the relative importance of tissue calcification and hyperpulsatility in the etiology of renal failure. VDN rats showed significant increases in aortic wall calcium content (50 times; 992+/-171 vs. control 19+/-1 micromol/g dry wt) and pulse pressure (1.5 times; 61+/-4 vs. control 40+/-2 mmHg). Significant renal calcification (16 times; 124+/-27 vs. control 8.1+/-0.7 micromol/g dry wt) occurred mainly within the media of the preglomerular vasculature and in the areas of interstitial fibrosis in VDN. Extensive renal damages (5 times; 26+/-5% of collapsed-atrophic or sclerotic glomeruli, or glomerular cysts vs. control 5.2+/-0.3%; 28 times; 61+/-12% areas of focal, cortical areas exhibiting interstitial fibrosis per section vs. control 2.2+/-0.6%) were observed histologically. The glomerular filtration rate significantly decreased (880+/-40 vs. control 1,058+/-44 microl.min(-1). g kidney wt(-1)). Albuminuria increased six times (1.6+/-0.4 vs. control 0.27+/-0.04 mg/24 h). There were significant linear relationships between albuminuria and pulse pressure (r2=0.408; n=24) or renal calcium content (r2=0.328; n=24; P<0.05) and between glomerular filtration rate and pulse pressure (r2=0.168; n=27). To our knowledge, this study provides the first evidence of links between both 1) hyperpulsatility and renal dysfunction, and 2) renal calcification and renal dysfunction. Given the increasing frequency of end-stage renal disease, this model could prove useful for preclinical evaluation of drugs that prevent or attenuate hyperpulsatility and/or tissue calcification.
Treatment and prognostic analysis of patients with leptomeningeal metastases from non-small cell lung cancer

Leptomeningeal metastases (LM) from non‐small cell lung cancer (NSCLC) are associated with poor prognosis and optimal treatment for this subgroup of NSCLC patients is controversial. The purpose of this study is to evaluate treatment options and prognostic factors of NSCLC patients with LM.
AutoEval mkII - Interaction Design for a VR Design Review System

This paper summarizes the experience drawn from designing and revising a design review application prototype interface using immersive virtual reality technology and putting it into context with previous research in the field of 3D human-computer interaction. AutoEval was originally developed in collaboration with a major car manufacturer to enable intuitive analysis and manipulation of 3D models for users without a CAD or computer science background. This paper introduces the system and discusses the 3D interaction design decisions taken based on the observation and informal feedback of a large number of users
Changes in the structural complexity of the aged brain

Structural changes of neurons in the brain during aging are complex and not well understood. Neurons have significant homeostatic control of essential brain functions, including synaptic excitability, gene expression, and metabolic regulation. Any deviations from the norm can have severe consequences as seen in aging and injury. In this review, we present some of the structural adaptations that neurons undergo throughout normal and pathological aging and discuss their effects on electrophysiological properties and cognition. During aging, it is evident that neurons undergo morphological changes such as a reduction in the complexity of dendrite arborization and dendritic length. Spine numbers are also decreased, and because spines are the major sites for excitatory synapses, changes in their numbers could reflect a change in synaptic densities. This idea has been supported by studies that demonstrate a decrease in the overall frequency of spontaneous glutamate receptor‐mediated excitatory responses, as well as a decrease in the levels of α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid and N‐methyl‐d‐aspartate receptor expression. Other properties such as γ‐aminobutyric acid A receptor‐mediated inhibitory responses and action potential firing rates are both significantly increased with age. These findings suggest that age‐related neuronal dysfunction, which must underlie observed decline in cognitive function, probably involves a host of other subtle changes within the cortex that could include alterations in receptors, loss of dendrites, and spines and myelin dystrophy, as well as the alterations in synaptic transmission. Together these multiple alterations in the brain may constitute the substrate for age‐related loss of cognitive function.
Adult Age Differences in Attention to Semantic Context Attention to Semantic

In three experiments age differences in attention to semantic context were examined. The performance of younger adults (ages 18–29 years) and older adults (ages 60–79 years) on a semantic priming task indicated that both age groups could use information regarding the probability that a prime and target would be related to flexibly anticipate the target category given the prime word (Experiment 1). The timing by which target expectancies were reflected in reaction time performance was delayed for older adults as compared to younger adults, but only when the target was expected to be semantically unrelated to the prime word (Experiment 2). When the target and prime were expected to be semantically related, the time course of priming effects was similar for younger and older adults (Experiment 3). Together the findings indicate that older adults are able to use semantic context and the probability of stimulus relatedness to anticipate target information. Although aging may be associated with a delay in the timing by which controlled expectancies are expressed, these findings argue against an age-related decline in the ability to represent contextual information.