Miracle-dz/UniFAR-FTUs huggingface dataset:
Xerocytosis is caused by mutations that alter the kinetics of the mechanosensitive channel PIEZO1

Significance Familial xerocytosis in humans, which causes dehydration of red blood cells and hemolytic anemia, was traced to mutations in the mechanosensitive ion channel, PIEZO1. The mutations slowed inactivation and introduced a pronounced latency for activation. Loss of inactivation and increased latency for activation could modify groups of channels simultaneously, suggesting that they exist in common spatial domains. The hereditary xerocytosis mutants affect red cell cation fluxes: slow inactivation increases them, and increased latency decreases them. These data provide a direct link between pathology and mechanosensitive channel dysfunction in nonsensory cells. Familial xerocytosis (HX) in humans is an autosomal disease that causes dehydration of red blood cells resulting in hemolytic anemia which has been traced to two individual mutations in the mechanosensitive ion channel, PIEZO1. Each mutation alters channel kinetics in ways that can explain the clinical presentation. Both mutations slowed inactivation and introduced a pronounced latency for activation. A conservative substitution of lysine for arginine (R2456K) eliminated inactivation and also slowed deactivation, indicating that this mutant’s loss of charge is not responsible for HX. Fitting the current vs. pressure data to Boltzmann distributions showed that the half-activation pressure, P1/2, for M2225R was similar to that of WT, whereas mutations at position 2456 were left shifted. The absolute stress sensitivity was calibrated by cotransfection and comparison with MscL, a well-characterized mechanosensitive channel from bacteria that is driven by bilayer tension. The slope sensitivity of WT and mutant human PIEZO1 (hPIEZO1) was similar to that of MscL implying that the in-plane area increased markedly, by ∼6–20 nm2 during opening. In addition to the behavior of individual channels, groups of hPIEZO1 channels could undergo simultaneous changes in kinetics including a loss of inactivation and a long (∼200 ms), silent latency for activation. These observations suggest that hPIEZO1 exists in spatial domains whose global properties can modify channel gating. The mutations that create HX affect cation fluxes in two ways: slow inactivation increases the cation flux, and the latency decreases it. These data provide a direct link between pathology and mechanosensitive channel dysfunction in nonsensory cells.
Efficient Algorithms for Public-Private Social Networks

We introduce the public-private model of graphs. In this model, we have a public graph and each node in the public graph has an associated private graph. The motivation for studying this model stems from social networks, where the nodes are the users, the public graph is visible to everyone, and the private graph at each node is visible only to the user at the node. From each node's viewpoint, the graph is just a union of its private graph and the public graph. We consider the problem of efficiently computing various properties of the graphs from each node's point of view, with minimal amount of recomputation on the public graph. To illustrate the richness of our model, we explore two powerful computational paradigms for studying large graphs, namely, sketching and sampling, and focus on some key problems in social networks and show efficient algorithms in the public-private graph model. In the sketching model, we show how to efficiently approximate the neighborhood function, which in turn can be used to approximate various notions of centrality. In the sampling model, we focus on all-pair shortest path distances, node similarities, and correlation clustering.
A controlled trial of desipramine in 18 men with posttraumatic stress disorder.

Eighteen male U.S. veterans meeting DSM-III criteria for posttraumatic stress disorder (PTSD) completed a 4-week double-blind, crossover study comparing administration of 200 mg/day of desipramine with placebo. Response was measured by using the Beck Depression Inventory, the Hamilton Rating Scale for Depression, the Hamilton Rating Scale for Anxiety, and the Impact of Event Scale. Overall, the only apparent response to desipramine was in some symptoms of depression; there were no changes in anxiety and other PTSD symptoms.
Pneumonia identification using statistical feature selection

OBJECTIVE This paper describes a natural language processing system for the task of pneumonia identification. Based on the information extracted from the narrative reports associated with a patient, the task is to identify whether or not the patient is positive for pneumonia. DESIGN A binary classifier was employed to identify pneumonia from a dataset of multiple types of clinical notes created for 426 patients during their stay in the intensive care unit. For this purpose, three types of features were considered: (1) word n-grams, (2) Unified Medical Language System (UMLS) concepts, and (3) assertion values associated with pneumonia expressions. System performance was greatly increased by a feature selection approach which uses statistical significance testing to rank features based on their association with the two categories of pneumonia identification. RESULTS Besides testing our system on the entire cohort of 426 patients (unrestricted dataset), we also used a smaller subset of 236 patients (restricted dataset). The performance of the system was compared with the results of a baseline previously proposed for these two datasets. The best results achieved by the system (85.71 and 81.67 F1-measure) are significantly better than the baseline results (50.70 and 49.10 F1-measure) on the restricted and unrestricted datasets, respectively. CONCLUSION Using a statistical feature selection approach that allows the feature extractor to consider only the most informative features from the feature space significantly improves the performance over a baseline that uses all the features from the same feature space. Extracting the assertion value for pneumonia expressions further improves the system performance.
REGRESSIVE ENTRY COSTS TO MARKET PARTICIPATION

There are transactions costs to participating in the marketplace. This paper argues that not only are transactions costs different across individuals, but that they are in fact regressive in their impact. In other words, the incidence of unintended consequences mediated through the market falls disproportionately on the very people who can ill afford to bear them. Such phenomenon can be partially traced to differences in personal capabilities and social location. Disadvantaged economic agents will find themselves having to pay higher “entry costs” to market participation because unfettered market operations favor those who have better human capital and are better situated. Thus, there is need for extra-market mechanisms to level the playing field and to provide relief and assistance to those who are unable to pay the “entry cost” to partaking in the market. Conference Proceedings Transforming Unjust Structures: Capabilities and Justice 26-27 June 2003, von Hügel Institute St Edmund's College Cambridge 1 REGRESSIVE ENTRY COSTS TO MARKET PARTICIPATION
A New Measure of Cognitive Flexibility

This paper contains a description of a scale to measure cognitive flexibility. In Study 1, 247 participants completed the Cognitive Flexibility Scale, the Communication Flexibility Scale, and the Rigidity of Attitudes Regarding Personal Habits Scale. In Study 2, 275 participants completed the Cognitive Flexibility Scale, the Interaction Involvement Scale, the Self-monitoring Scale, and the Unwillingness to Communicate Scale. Analysis showed the Cognitive Flexibility Scale to be internally reliable and supported its construct and concurrent validity.
Teachers in the news: a critical analysis of one US newspaper's discourse on education, 2006–2007

Public education discourse in the USA has been characterized by messages of crisis shaping education policies across national contexts. Education policy solutions target a lack of qualified teachers and insufficient oversight of teacher practice as central factors in the crisis, placing teacher identity as knowledgeable, authoritative professionals at the center of educational reform debates. Mainstream news media is a key site for education policy debate. I employ critical discourse analysis of education news published in a major US newspaper, uncovering how grammar patterns in news discourse situate teacher identity in relation to knowledge and authority. I demonstrate how the paper's discourse frames teacher identity in terms of Accountability and Caring and discuss how, in the context of larger economic and social policy debates, Accountability gains authority over caring to shape education policy. I call for teachers to integrate critical participation in public education debates as key element of professional identity.
The zebrafish as a model system for analyzing mammalian and native α-crystallin promoter function

Previous studies have used the zebrafish to investigate the biology of lens crystallin proteins and their roles in development and disease. However, little is known about zebrafish α-crystallin promoter function, how it compares to that of mammals, or whether mammalian α-crystallin promoter activity can be assessed using zebrafish embryos. We injected a variety of α-crystallin promoter fragments from each species combined with the coding sequence for green fluorescent protein (GFP) into zebrafish zygotes to determine the resulting spatiotemporal expression patterns in the developing embryo. We also measured mRNA levels and protein abundance for all three zebrafish α-crystallins. Our data showed that mouse and zebrafish αA-crystallin promoters generated similar GFP expression in the lens, but with earlier onset when using mouse promoters. Expression was also found in notochord and skeletal muscle in a smaller percentage of embryos. Mouse αB-crystallin promoter fragments drove GFP expression primarily in zebrafish skeletal muscle, with less common expression in notochord, lens, heart and in extraocular regions of the eye. A short fragment containing only a lens-specific enhancer region increased lens and notochord GFP expression while decreasing muscle expression, suggesting that the influence of mouse promoter control regions carries over into zebrafish embryos. The two paralogous zebrafish αB-crystallin promoters produced subtly different expression profiles, with the aBa promoter driving expression equally in notochord and skeletal muscle while the αBb promoter resulted primarily in skeletal muscle expression. Messenger RNA for zebrafish αA increased between 1 and 2 days post fertilization (dpf), αBa increased between 4 and 5 dpf, but αBb remained at baseline levels through 5 dpf. Parallel reaction monitoring (PRM) mass spectrometry was used to detect αA, aBa, and αBb peptides in digests of zebrafish embryos. In whole embryos, αA-crystallin was first detected by 2 dpf, peaked in abundance by 4–5 dpf, and was localized to the eye. αBa was detected in whole embryo at nearly constant levels from 1–6 dpf, was also localized primarily to the eye, and its abundance in extraocular tissues decreased from 4–7 dpf. In contrast, due to its low abundance, no αBb protein could be detected in whole embryo, or dissected eye and extraocular tissues. Our results show that mammalian α-crystallin promoters can be efficiently screened in zebrafish embryos and that their controlling regions are well conserved. An ontogenetic shift in zebrafish aBa-crystallin promoter activity provides an interesting system for examining the evolution and control of tissue specificity. Future studies that combine these promoter based approaches with the expanding ability to engineer the zebrafish genome via techniques such as CRISPR/Cas9 will allow the manipulation of protein expression to test hypotheses about lens crystallin function and its relation to lens biology and disease.
The impact of larval and juvenile fish on zooplankton and algal dynamics

The impact of young‐of‐the‐year (YOY) fish predation on zooplankton dynamics and cascading effects down to phytoplankton were studied in lake enclosures using different densities of larval and juvenile European perch (Perca fluviatilis). Two experiments, one in June and one in August, were performed in Lake Dagstorpssjon, a slightly eutrophic lake in Scania, southern Sweden. In our experiments, we found that in August, there was a negative relation between YOY perch density and cladoceran biomass and between YOY perch density and cladoceran size. Moreover, in August, both rotifer and algal abundance increased with increasing YOY density, and species composition of algae was affected. At high YOY densities, there was also a negative effect on water transparency. In June, when growth of larval perch was slow and daphnid abundance low, larval perch was not the major structuring force on either zooplankton or algal communities. In early summer, the likelihood of YOY perch having an impact on cladoceran biomass is dependent on the timing of many factors. One factor is the timing between larval occurrence and the increase in large cladoceran biomass that will enhance larval growth. In late summer, however, a high enough predation pressure of juvenile fish will prevent recovery of large herbivores after the midsummer decline in zooplankton abundance and will drive the algal community toward a late summer species composition of small algal cells and blue‐green algae.
Dawes Review 4: Spiral Structures in Disc Galaxies

Abstract The majority of astrophysics involves the study of spiral galaxies, and stars and planets within them, but how spiral arms in galaxies form and evolve is still a fundamental problem. Major progress in this field was made primarily in the 1960s, and early 1970s, but since then there has been no comprehensive update on the state of the field. In this review, we discuss the progress in theory, and in particular numerical calculations, which unlike in the 1960s and 1970s, are now commonplace, as well as recent observational developments. We set out the current status for different scenarios for spiral arm formation, the nature of the spiral arms they induce, and the consequences for gas dynamics and star formation in different types of spiral galaxies. We argue that, with the possible exception of barred galaxies, spiral arms are transient, recurrent and initiated by swing amplified instabilities in the disc. We suppose that unbarred m = 2 spiral patterns are induced by tidal interactions, and slowly wind up over time. However the mechanism for generating spiral structure does not appear to have significant consequences for star formation in galaxies.
Competence is not enough: meta-competence and accounting education

The UK Management Charter Intiative (MCI) movement towards competence-based education is an indication of the growing attention being paid to the notion of the competent professional. There are already developments by the UK accounting bodies to develop detailed programmes to introduce competence-based education to accounting students. Competences have been defined in various ways - a behavioural description of workplace performance; doing rather than knowing; a focus on output (what a person can do) rather than input (what he or she has been taught). The underlying notion is to provide specific skills that are relevant to professional practice. The paper suggests that competences are not enough, and that what is required - meta-competence - can be learned but cannot be taught. Meta-competence is the overarching ability under which competence shelters. It embraces the higher order abilities which have to do with being able to learn, adapt, anticipate and create. Meta-competences are a prerequisite for the.
Leadership and job satisfaction among aviation fire fighters in Australia

Recently important changes have been made to fire service management practices in Australia; however, these changes have typically not been based on empirical research or followed up by systematic evaluation. While job satisfaction and leadership behaviour have been extensively investigated in other fields, studies of these variables in the fire service, particularly in Australia, are almost absent. This article examines links between job satisfaction and leadership behaviour in aviation fire fighters. Fifty‐six fire fighters responded to the Job Descriptive Index and the Leadership Behaviour Questionnaire XII. As hypothesised, employee job satisfaction correlated with supervisor leadership behaviour, with the exception of consideration leadership and co‐worker satisfaction. A new explanation, concerning level of team interaction, is proposed for the findings. Implications for the fire service and areas for further research are suggested.
High-redshift Faranoff-Riley type II radio galaxies: X-ray properties of the cores

We present an extensive X-ray spectral analysis of the cores of 19 Faranoff-Riley type II sources in the redshift range 0.5 < z < 1.0 which were selected to be matched in isotropic radio power. The sample consists of 10 radio galaxies (RGs) and nine quasars. We compare our results with the expectations from a unification model that ascribes the difference between these two types of sources to the viewing angle to the line of sight, beaming and the presence of a dust and gas torus. We find that the spectrum of all the quasars can be fitted with a single power law, and that the spectral index flattens with decreasing angle to the line of sight. We interpret this as the effect of increasingly dominant inverse Compton X-ray emission, beamed such that the jet emission outshines other core components. For up to 70 per cent of the RGs we detect intrinsic absorption; their core spectra are best fitted with an unabsorbed steep power law of average spectral index r = 2.1 and an absorbed power law of spectral index r = 1.6, which is flatter than that observed for radio-quiet quasars (RQQs). We further conclude that the presence of a jet affects the spectral properties of absorbed nuclear emission in active galactic nuclei. In RGs, any steep-spectrum component of nuclear X-ray emission, similar to that seen in RQQs, must be masked by a jet or by jet-related emission.
A Case-Based Approach to Transition of Care for Patients With Sickle Cell Disease

Abstract Young adults with sickle cell disease must navigate a difficult road to independence once they age out of pediatric care. The anxiety surrounding transition, the challenges of medical complications, and chronic psychosocial stressors are obstacles to a seamless transition to adult medical care. The two cases presented here demonstrate that a team-based, multidisciplinary approach can facilitate a successful transition.
mRNA Expression of MT1-MMP, MMP-9, Cathepsin K, and TRAP in Highly Enriched Osteoclasts Cultured on Several Matrix Proteins and Ivory Surfaces

We demonstrated that the transcriptional expression of MT1-MMP, MMP-9, cathepsin K, and TRAP in highly enriched osteoclasts were regulated by different matrix proteins that bind to integrin on osteoclast, such as collagen type I (CoI), fibronectin (FN), vitronectin (VN), osteopontin (OPN), and ivory. Results suggested that the OPN-integrin αVβ3 binding plays a more important role than CoI-α2β1 binding in the regulation of osteoclast activity.
Seizure outcome after epilepsy surgery in children and adolescents

Few epilepsy surgery outcome data are available from series of pediatric patients. We studied seizure outcome in 136 pediatric patients who had surgery for intractable epilepsy at The Cleveland Clinic between January 1990 and June 1996, with a postoperative follow‐up of 1 to 7.5 years (mean, 3.6 years). Sixty‐two children (3 months to 12 years old at time of surgery) were compared with 74 adolescents (13–20 years old). Extratemporal or multilobar resections and hemispherectomies were similarly frequent among children (50%) and adolescents (44%), but these procedures strongly predominated in infancy (90% of patients 0–2 years of age). The remaining patients had temporal resection. Cortical dysplasia and low‐grade tumor were the most common causes and hippocampal sclerosis was rare. Seizure‐free outcome was achieved for 69% of adolescents, 68% of children, and 60% of the infant subgroup, overall; for 23 (74%) of 31 children and 33 (80%) of 41 adolescents after temporal resection; for 11 (58%) of 19 children and 15 (52%) of 29 adolescents after extratemporal or multilobar resection; and for 8 (67%) of 12 children and 3 (75%) of 4 adolescents after functional hemispherectomy. Seizure‐free outcome was more frequent after temporal resection (56 of 72, 78%) than after extratemporal or multilobar resection (26 of 48, 54%; 41 of 48 with a focal lesion on magnetic resonance imaging), and among patients with tumor (36 of 44, 82%) versus cortical dysplasia (16 of 31, 52%). The frequency of seizure‐free outcome after epilepsy surgery was similar for infants, children, and adolescents, and comparable with results from adult series. Most patients in each age, surgery type, and causal group were free from seizures after surgery. These results suggest that children should be considered for surgical evaluation at whatever age they manifest with severe, intractable, disabling localization‐related epilepsy.
Cobas-Bact system for identification of members of the family Enterobacteriaceae in 4 h 20 min

The Cobas-Bact (Roche Diagnostics, Basel, Switzerland) new rotor for the identification (ID) in 4 h 20 min of 33 members of the family Enterobacteriaceae to genus and species level was evaluated by testing 444 strains of which 398 belonged to common species and 46 belonged to rare species of Enterobacteriaceae. Each strain was identified by the API 20E system (Analytab Products, Plainview, N.Y.), and additional discriminating tests were set up if necessary. Only first-choice ID were considered in this study and were classified either as high-confidence ID (normalized likelihood, greater than or equal to 80%) or as low-confidence ID (normalized likelihood, less than 80%) requiring additional tests for confirmation. The data were analyzed by two versions of Cobas-Bact software. With the first version of the software (SW8446), the overall accuracy of Cobas-Bact was 95.5% (424 correct ID of 444). When restricted to high-confidence ID it rose to 99.4% (350 of 352) for the common species and 96.9% (31 of 32) for the rare species. Only three strains of the high-confidence group were misidentified. Sixty ID were considered unacceptable because of their low confidence. Using the first software version (SW8446) they represented 12% (46 of 398) of the common species (17 typical strains, 10 Shigella species, 10 inactive Escherichia coli strains, and 9 rare biotypes) and 30% (14 of 46) of the rare species. The same data analyzed by the new version (SW8524) of the Cobas-Bact software resulted in an overall accuracy of 93.9% (417 correct ID). The number of high-confidence ID rose to 401, of which 392 (97.7%) were accurate. The decrease in low-confidence ID (43 versus 60) was mainly due to the Shigella species. In conclusion the accuracy of Cobas-Bact identification system was very good when restricted to high-confidence ID. The Cobas-Bact performance for rare species ID was poorer, but the small number of strains tested does not allow definitive conclusions.