Miracle-dz/UniFAR-FTUs huggingface dataset:
Aberrant Expression of Novel Cytokine IL-38 and Regulatory T Lymphocytes in Childhood Asthma

We investigated the expression of novel anti-inflammatory interleukin (IL)-38 and regulatory T (Treg) lymphocytes in childhood asthma patients. The protein and mRNA expression level of IL-38, periostin, peripheral CD4+CD25+CD134+ T lymphocytes as well as CD4+CD25highFoxP3+ and CD4+CD25highCD127− Treg lymphocytes from 40 asthmatic patients and 20 normal control (NC) subjects were studied using ELISA, qPCR and flow cytometry. Serum and supernatant cytokines/chemokines were determined by multiplex assay. Serum IL-38, IL-5, IL-17, IL-6, interferon-γ, periostin, IL-1β and IL-13 concentrations were significantly higher in asthmatic patients with or without steroid treatment than those in controls (all p < 0.05). The percentages of both CD4+CD25highFoxP3+ and CD4+CD25highCD127− Treg lymphocytes were markedly decreased in asthmatic patients with and without steroid treatment than those in controls (all p < 0.05). The elevated IL-38 concentration negatively correlated with the percentage of Treg lymphocytes in asthmatic patients with high level (>40 ng/mL) of periostin (p < 0.05). Although the comparable mRNA levels of IL-38 and its receptor IL-36R were found between patients and controls, the mRNA level of IL-38 positively correlated with IL-36R and negatively correlated with IL-10 in all asthmatic patients (both p < 0.05). The percentage of CD4+CD25+CD134+ activated T lymphocytes was also significantly higher in asthmatic patients with steroid treatment than those in controls (p < 0.05). This cross-sectional study demonstrated that the overexpression of circulating IL-38 may play a role in the immunopathogenesis in asthma.
Dendrimer-Based Selective Proteostasis-Inhibition Strategy to Control NSCLC Growth and Progression

Elevated valosin containing protein (VCP/p97) levels promote the progression of non-small cell lung carcinoma (NSCLC). Although many VCP inhibitors are available, most of these therapeutic compounds have low specificity for targeted tumor cell delivery. Hence, the primary aim of this study was to evaluate the in vitro efficacy of dendrimer-encapsulated potent VCP-inhibitor drug in controlling non-small cell lung carcinoma (NSCLC) progression. The VCP inhibitor(s) (either in their pure form or encapsulated in generation-4 PAMAM-dendrimer with hydroxyl surface) were tested for their in vitro efficacy in modulating H1299 (NSCLC cells) proliferation, migration, invasion, apoptosis and cell cycle progression. Our results show that VCP inhibition by DBeQ was significantly more potent than NMS-873 as evident by decreased cell proliferation (p<0.0001, MTT-assay) and migration (p<0.05; scratch-assay), and increased apoptosis (p<0.05; caspase-3/7-assay) as compared to untreated control cells. Next, we found that dendrimer-encapsulated DBeQ (DDNDBeQ) treatment increased ubiquitinated-protein accumulation in soluble protein-fraction (immunoblotting) of H1299 cells as compared to DDN-control, implying the effectiveness of DBeQ in proteostasis-inhibition. We verified by immunostaining that DDNDBeQ treatment increases accumulation of ubiquitinated-proteins that co-localizes with an ER-marker, KDEL. We observed that proteostasis-inhibition with DDNDBeQ, significantly decreased cell migration rate (scratch-assay and transwell-invasion) as compared to the control-DDN treatment (p<0.05). Moreover, DDNDBeQ treatment showed a significant decrease in cell proliferation (p<0.01, MTT-assay) and increased caspase-3/7 mediated apoptotic cell death (p<0.05) as compared to DDN-control. This was further verified by cell cycle analysis (propidium-iodide-staining) that demonstrated significant cell cycle arrest in the G2/M-phase (p<0.001) by DDNDBeQ treatment as compared to control-DDN. Moreover, we confirmed by clonogenic-assay that DDNDBeQ treatment significantly (p<0.001) inhibits H1299 colony-formation as compared to control/DDN. Overall, encapsulation of potent VCP-inhibitor DBeQ into a dendrimer allows selective VCP-mediated proteostasis-inhibition for controlling NSCLC-tumor growth and progression to allow tumor-targeted sustained drug delivery.
Ziv–Zakai Bounds on Time Delay Estimation in Unknown Convolutive Random Channels

Using the Ziv-Zakai bound (ZZB) methodology, we develop a Bayesian mean-square error bound on time delay estimation (TDE) in convolutive random channels, and compare it with time delay estimator performance and a Crame¿r-Rao bound. The channel is modeled as a tapped delay line, whose taps are Gaussian random variables that may be nonzero mean and correlated, a model widely adopted in many applications such as wideband fading in a multipath channel. The time delay has a uniform prior distribution. A ZZB is developed that incorporates the prior on the random time delay, as well as the convolutive random Gaussian channel, and does not assume the receiver has knowledge of the channel realization. The ZZB provides good performance prediction for maximum a posteriori (MAP) time delay estimation, tracking the low, medium, and high signal-to-noise ratio (SNR) regimes. The convolutive channel model includes important special cases, such as narrowband Gaussian channels corresponding to Rayleigh/Rician fading, wideband multipath channels with a power decay profile (such as exponential decay), and known channels. We show that the associated Crame¿r-Rao bound is tight only at high SNR, whereas the ZZB predicts threshold behavior and TDE breakdown as the SNR decreases. When compared to a ZZB that assumes knowledge of the channel realization, the ZZB developed here provides a more realistic and tighter bound, revealing the performance loss due to lack of channel knowledge. The MAP estimator incorporates knowledge of the channel statistics, and so performs much better than a maximum likelihood estimator that minimizes mean square error but does not use knowledge of the random channel statistics. Several examples illustrate the estimator and bound behaviors.
Aquaporins in the Colon as a New Therapeutic Target in Diarrhea and Constipation

Aquaporins (AQPs) play important roles in the water transport system in the human body. There are currently 13 types of AQP, AQP0 through AQP12, which are expressed in various organs. Many members of the AQP family are expressed in the intestinal tract. AQP3 is predominantly expressed in the colon, ultimately controlling the water transport. Recently, it was clarified that several laxatives exhibit a laxative effect by changing the AQP3 expression level in the colon. In addition, it was revealed that morphine causes severe constipation by increasing the AQP3 expression level in the colon. These findings have shown that AQP3 is one of the most important functional molecules in water transport in the colon. This review will focus on the physiological and pathological roles of AQP3 in the colon, and discuss clinical applications of colon AQP3.
Is there a safe limit of delay for emergency caesarean section in Ghana? Results of analysis of early perinatal outcome.

OBJECTIVE To determine the limits of delaying caesarean section in a busy obstetric unit in a developing country setting that is not associated with neonatal survival. METHODS Retrospective cohort study of emergency cesarean sections. Indications were sub-divided into imminent threat and no imminent threat to fetal wellbeing. The primary outcomes was a composite measure of adverse perinatal outcome including stillbirth, 5-minute Apgar score < 7 and neonatal intensive care unit admission. Effect of decision-to-delivery interval on perinatal outcomes was evaluated using Kaplan-Meier survival analysis. RESULTS 495 women met inclusion criteria (142 'imminent threat' group, 353 'no imminent threat' group). The median decision-to-delivery interval was significantly shorter in the 'imminent threat' group (2.25 [95% CI 1.38 - 5.83] versus 3.42 [95% CI 1.83 - 5.85] hours, p <0.001). Only 1.7% and 12.7% sections were performed within 30 minutes and 1 hour, respectively. Risk of the composite outcome was significantly higher in the 'imminent threat group (46.5% versus 31.2%, RR=1.49 [95% CI 1.18 - 1.89], p=0.001). A 95% probability of 'live intact' survival occurred at 1 hr and 2 hrs respectively, for the imminent threat and the no imminent threat groups. CONCLUSION Increasing decision-to-delivery interval is associated with higher risk of adverse perinatal outcomes, but a 95% live intact survival can be achieved if the delivery occurs within 2 hours.
Fortalecimiento de competencias básicas en ciencias naturales desde el Modelo Pedagógico de la Formación Profesional Integral en el Centro Nacional de Hotelería, Turismo e Industrias Alimentarias, regional Bogotá (CNHTyA)

This article gives an account on a work that sought to strengthen basic skills in natural sciences in the CNHTyA. Between 2017 and 2018, a descriptive study of a case study was carried out with a quantitative approach, designing and applying a learning guide for the development of basic skills in Natural Sciences. The strategies employed increased the skill level by 40%. The optimization of the development of competences was given by prioritizing the relationship of affective, cognitive and metacognitive levels in professional training, above psychometric principles.
Robust direction estimation of UWB sources in Laguerre-Gauss beamspaces

A novel method for estimating the direction of arrival of ultra-wideband wavefronts impinging on a linear uniform sensor array is proposed. It is based on expanding the space time signals onto a basis of Laguerre-Gauss Circular Harmonic functions, whose peculiar properties lead to a new signal subspace parametric model. This approach allows robust angle of arrival estimation in low signal to noise ratio environments.
Genotyping and Molecular Characterization of Hepatitis B Virus from Human Immunodeficiency Virus-Infected Individuals in Southern Africa

Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) are hyperendemic in sub-Saharan Africa. The HBV genotypes prevailing in HIV-infected Africans are unknown. Our aim was to determine the HBV genotypes in HIV-infected participants and to identify clinically significant HBV mutations. From 71 HBV DNA+ve HIV-infected participants, 49 basic core promoter/precore (BCP/PC) and 29 complete S regions were successfully sequenced. Following phylogenetic analysis of 29 specimens in the complete S region, 28 belonged to subgenotype A1 and one to D3. Mutations affecting HBeAg expression at the transcriptional (1762T1764A), translational (Kozak 1809–1812, initiation 1814–1816, G1896A with C1858T), or post translational levels (G1862T), were responsible for the high HBeAg-negativity observed. The G1862T mutation occurred only in subgenotype A1 isolates, which were found in one third (7/21) of HBsAg−ve participants, but in none of the 18 HBsAg+ve participants (p<0.05). Pre-S deletion mutants were detected in four HBsAg+ve and one HBsAg−ve participant/s. The following mutations occurred significantly more frequently in HBV isolated in this study than in strains of the same cluster of the phylogenetic tree: ps1F25L, ps1V88L/A; ps2Q10R, ps2 R48K/T, ps2A53V and sQ129R/H, sQ164A/V/G/D, sV168A and sS174N (p<0.05). ps1I48V/T occurred more frequently in females than males (p<0.05). Isolates with sV168A occurred more frequently in participants with viral loads >200 IU per ml (p<0.05) and only sS174N occurred more frequently in HBsAg−ve than in HBsAg+ve individuals (p<0.05). Prior to initiation of ART, ten percent, 3 of 29 isolates sequenced, had drug resistance mutations rtV173L, rtL180M+rtM204V and rtV214A, respectively. This study has provided important information on the molecular characteristics of HBV in HIV-infected southern Africans prior to ART initiation, which has important clinical relevance in the management of HBV/HIV co-infection in our unique setting.
Temporal Dynamics of Neural Adaptation Effect in the Human Visual Ventral Stream

When the same visual stimulus is repeatedly presented with a brief interval, the brain responses to that stimulus are attenuated relative to those at first presentation [neural adaptation (NA)]. Although this effect has been widely observed in various regions of human brain, its temporal dynamics as a neuronal population has been mostly unclear. In the present study, we used a magnetoencephalography (MEG) and conducted a macrolevel investigation of the temporal profiles of the NA occurring in the human visual ventral stream. The combination of MEG with our previous random dot blinking method isolated the neural responses in the higher visual cortex relating to shape perception. We dissociated three dimensions of the NA: activation strength, peak latency, and temporal duration of neural response. The results revealed that visual responses to the repeated compared with novel stimulus showed a significant reduction in both activation strength and peak latency but not in the duration of neural processing. Furthermore, this acceleration of peak latency showed a significant correlation with reaction time of the subjects, whereas no correlation was found between the reaction time and the temporal duration of neural responses. These results indicate that (1) the NA involves the brain response changes in the temporal domain as well as the response attenuation reported previously, and (2) this temporal change is primarily observed as a rapid rising of “what” responses, rather than a temporal shortening of neural response curves within the visual ventral stream as considered previously.
Policies for Mixed Communities

The belief that it is fairer if communities are ``mixed'' can be traced at least to the late nineteenth century and the founders of the Garden City Movement. The idea is now firmly established in Organization for Economic Cooperation and Development (OECD) and national policies. This article reviews the evidence and argues that this is essentially a faith-based policy because there is scant real evidence that making communities more mixed makes the life chances of the poor any better. There is overwhelming evidence that the attributes that make neighborhoods attractive are capitalized into house prices/rents. The result is that poor people cannot afford to buy into nicer neighborhoods, which anyway have amenities of no value to them. Moreover, ``specialized neighborhoods'' are an important element in agglomeration economies and seem to be welfare enhancing. Thus, policies for mixed neighborhoods treat the symptoms rather than the causes of poverty. Efforts to improve social equity would be more effectively directed toward people themselves rather than moving people around to mix neighborhoods.
Structure based molecular inhibition of Caspase-8 for treatment of multi-neurodegenerative diseases using known natural compounds

Neurodegenerative disorders are often associated with excessive neuronal apoptosis. It is well known that apoptosis is regulated by some intracellular proteases, such as, Caspases (cysteine-dependent, aspartate-specific proteases). In fact, Caspase-8 which is an initiator caspase, has been identified as a key mediator of neuronal apoptosis. In addition, Caspase-8 is found to be coupled with the regulation of various neurodegenerative disorders including Alzheimer׳s disease (AD), Parkinson׳s disease (PD), Huntington׳s Diseases (HD) and Dentatorubral Pallidoluysian Atrophy (DRPLA). Caspase-8 inhibition may provide an effective means of treatment for multiple neurodegenerative disorders. Therefore, the present study describes the molecular interaction of some selected natural compounds with known anti neurodegenerative properties with Caspase-8. Docking between Caspase-8 and each of these compounds (separately) was performed using ‘Autodock4.2’. Out of all the selected compounds, rosmarinic acid and curcumin proved to be the most potent inhibitors of Caspase-8 with binding energy (ΔG) of -7.10 Kcal/mol and -7.08 Kcal/mol, respectively. However, further in vitro and in vivo studies are needed to validate the anti-neurodegenerative potential of these compounds.
Adaptation in the Corticosterone and Hyperthermic Responses to Stress Following Repeated Stressor Exposure

Previous studies have shown that repeated daily exposure to the same (homotypic) stressor results in habituation of the corticosterone (CORT) response. Others have found that the stress response to a more ethologically relevant stressor, social defeat, does not habituate and, in some cases, sensitisation has been observed. Similar observations have been noted when core temperature is examined. Although habituation and/or sensitisation have been reported during stressor exposure, little is known about the development of an anticipatory fever in response to daily stressor exposure. The aim of the present study was to compare systematically commonly used laboratory stressors (i.e. restraint, cage confinement and social defeat) using a common set of procedures and analyses. Specifically, we examined: (i) the development of an anticipatory fever to repeated (5 days) homotypic stressor exposure; (ii) the adaptation of the fever response during stressor exposure; and (iii) the resolution of the fever response to stressors presented at the same time each day. For comparison, adaptation of the CORT response was also examined to assess the degree to which habituation to repeated stressor exposure may represent a more general response observed across diverse physiological measures. Habituation was observed after restraint and cage confinement, but not observed in either the CORT or hyperthermic responses to repeated social defeat. Furthermore, no anticipatory fever response was observed with repeated exposure to restraint, cage confinement, or social defeat. These data suggest that habituation to repeated stressor exposure may not occur with all homotypic stressor paradigms. In addition, rats do not appear to entrain an anticipatory fever response to a stressor presented at the same time each day, at least not within 5–6 days of repeated exposure.
Complaints about sleep in trauma patients in an emergency department in respect to alcohol use.

UNLABELLED Among other lifestyle problems, sleeping problems have been related to alcohol use. AIM The aim of this study was to evaluate complaints about sleep in trauma patients in an emergency department and its relation to alcohol use disorders (AUD). METHOD In an emergency department, trauma patients (1014 females, 1680 males) were screened using a computerized questionnaire addressing AUD (AUD identification test, AUDIT), smoking, drug and medication use, and sleep (presence of difficulties in falling asleep and/or sleeping through). Age, BMI, income, employment, partner status, and "feeling fit" were additionally assessed. RESULTS An AUDIT score > or = 8 points was found in 24.2% of the males and 8.3% of the females. Complaints about sleep were reported by 28% and 34% of the patients, respectively. These complaints about sleep were more likely in males at-risk drinkers (AUDIT > or = 8 versus 1-4 points (Adjusted odds ratio: AOR = 1.60, P = 0.001) or abstainers (AUDIT = 0 versus 1-4 points, AOR = 1.55, P = 0.029), and with increasing age (AOR = 1.01, P = 0.010), in patients not feeling "fit" (AOR = 2.15, P < 0.001), and users of pain (AOR = 2.24, P < 0.001) and sleep medication (AOR = 8.03, P < 0.001). In females, complaints about sleep were more likely with higher age (AOR = 1.04, P = 0.023), higher BMI (AOR = 1.04, P = 0.023), with not-feeling-fit (AOR = 1.87, P < 0.001) and sleep medication (AOR = 5.24, P < 0.001), and less likely in patients with a higher education (AOR = 0.57, P < 0.001). CONCLUSION Complaints about sleep were reported frequently by trauma patients. There was an association between AUDs and sleep complaints (mainly problems about sleeping through) in males, but not in females.
Generating Incremental Length Summary Based on Hierarchical Topic Coverage Maximization

Document summarization is playing an important role in coping with information overload on the Web. Many summarization models have been proposed recently, but few try to adjust the summary length and sentence order according to application scenarios. With the popularity of handheld devices, presenting key information first in summaries of flexible length is of great convenience in terms of faster reading and decision-making and network consumption reduction. Targeting this problem, we introduce a novel task of generating summaries of incremental length. In particular, we require that the summaries should have the ability to automatically adjust the coverage of general-detailed information when the summary length varies. We propose a novel summarization model that incrementally maximizes topic coverage based on the document’s hierarchical topic model. In addition to the standard Rouge-1 measure, we define a new evaluation metric based on the similarity of the summaries’ topic coverage distribution in order to account for sentence order and summary length. Extensive experiments on Wikipedia pages, DUC 2007, and general noninverted writing style documents from multiple sources show the effectiveness of our proposed approach. Moreover, we carry out a user study on a mobile application scenario to show the usability of the produced summary in terms of improving judgment accuracy and speed, as well as reducing the reading burden and network traffic.
Distortion and Evolution of a Localized Vortex in an Irrotational Flow

This paper examines the interaction of an axisymmetric vortex monopole, such as a Lamb vortex, with a background irrotational flow. At leading order, the monopole is advected with the background flow velocity at the center of vorticity. However, inhomogeneities of the flow will cause the monopole to distort. It is shown that a shear‐diffusion mechanism, familiar from the study of mixing of passive scalars, plays an important role in the evolution of the vorticity distribution. Through this mechanism, nonaxisymmetric vorticity perturbations which do not shift the center of vorticity are homogenized along streamlines on a Re1/3 time scale, much faster than the Re decay time scale of an axisymmetric monopole. This separation of time scales leads to the quasisteady evolution of a monopole in a slowly varying flow. The asymptotic theory is verified by numerically computing the linear response of a Lamb monopole to a time‐periodic straining flow and it is shown that a large amplitude, O(Re1/3), distortion resul.