Miracle-dz/UniFAR-FTUs huggingface dataset:
Effectiveness of haemodiailtration with ultrailtrate regeneration in the reduction of light chains in multiple myeloma with renal failure

Acute kidney failure in multiple myeloma (MM) occurs in 12%-20% of patients and is a poor prognostic factor for patient survival. Recent studies have shown that dialysis with a High-Cut-Off membrane (HCO) removes free light chains (FLC) effectively although with significant albumin loss. Other adsorption-based techniques, such as haemodiafiltration with ultrafiltrate regeneration by adsorption in resin (SUPRA-HFR), have not been studied. We present three cases of MM, all haemodialysisdependent since diagnosis. Two cases were IgG kappa and one was IgA lambda. All patients were treated with chemotherapy and SUPRA-HFR. The aim of this study was to evaluate the effectiveness of SUPRA-HFR in the reduction of FLC and its effect on albumin. We collected blood samples preand post-dialysis, and ultrafiltrate (UF) samples preand post-resin 5 minutes into the session and 5 minutes from the end. The mean reduction rate of FLC in blood per session in the three patients was 53% and 63% (kappa) and 38% (lambda). In the UF, the mean FLC reduction rate was close to 99%, both at the start and at the end of dialysis, without the removal of albumin. With the results obtained we can conclude that this technique achieves an effective reduction of FLC, which is maintained throughout the session, without resin saturation and without albumin loss. Therefore, SUPRA-HFR is effective as an adjunctive therapy for MM.
The Development, Description and Appraisal of an Emergent Multimethod Research Design to Study Workforce Changes in Integrated Care Interventions

Introduction: In this paper, we provide a detailed and explicit description of the processes and decisions underlying and shaping the emergent multimethod research design of our study on workforce changes in integrated chronic care. Theory and methods: The study was originally planned as mixed method research consisting of a preliminary literature review and quantitative check of these findings via a Delphi panel. However, when the findings of the literature review were not appropriate for quantitative confirmation, we chose to continue our qualitative exploration of the topic via qualitative questionnaires and secondary analysis of two best practice case reports. Results: The resulting research design is schematically described as an emergent and interactive multimethod design with multiphase combination timing. In doing so, we provide other researchers with a set of theory- and experience-based options to develop their own multimethod research and provide an example for more detailed and structured reporting of emergent designs. Conclusion and discussion: We argue that the terminology developed for the description of mixed methods designs should also be used for multimethod designs such as the one presented here.
Genetics of personalities: no simple answers for complex traits

Identifying the genes that underlie phenotypic variation in natural populations, and assessing the consequences of polymorphisms at these loci for individual fitness are major objectives in evolutionary biology. Yet, with the exception of a few success stories, little progress has been made, and our understanding of the link between genotype and phenotype is still in its infancy. For example, although body length in humans is largely genetically determined, with heritability estimates greater than 0.8, massive genome‐wide association studies (GWAS) have only been able to account for a very small proportion of this variation ( Gudbjartsson et al. 2008 ). If it is so difficult to explain the genetics behind relatively ‘simple’ traits, can we envision that it will at all be possible to find genes underlying complex behavioural traits in wild non‐model organisms? Some notable examples suggest that this can indeed be a worthwhile endeavour. Recently, the circadian rhythm gene Clock has been associated with timing of breeding in a wild blue tit population ( Johnsen et al. 2007 ; Liedvogel et al. 2009 ) and the Pgi gene to variation in dispersal and flight endurance in Glanville fritillary butterflies ( Niitepold et al. 2009 ). A promising candidate gene for influencing complex animal personality traits, also known as behavioural syndromes ( Sih et al. 2004 ), is the dopamine receptor D4 (DRD4) gene. Within the last decade, polymorphisms in this gene have been associated with variation in novelty seeking and exploration behaviour in a range of species, from humans to great tits ( Schinka et al. 2002 ; Fidler et al. 2007 ). In this issue, Korsten et al. (2010) attempt to replicate this previously observed association in wild‐living birds, and test for the generality of the association between DRD4 and personality across a number of European great tit populations.
Perubahan Tutupan Hutan Mangrove Di Pesisir Kabupaten Lampung Timur

The purposes of this study were: (1)to describe the change of mangrove forest cover along the coast of East Lampung Regency using Landsat data for 1973, 1983, 1994, 2004, and 2013; (2)todetermine triggering factors that occur in the coast of East Lampung Regency during the years 1973-2013 related to the mangrove forest change. The methods used in the study are spatial analysis and qualitative descriptive method. The study was conducted along the coast of East Lampung Regency over period of October 2013-March 2014.The result shows that: in 1973-1983 increased by 2.541,22 ha, in 1983-1994 decreased by 4.903,54 ha, in 1994-2004 decreased by 6.377,11 ha, in 2004-2013 decreased by 3.059,23 ha. The related factors that occur in there werechanges in the status management area is happening in Way Kambas National Park (TNWK), there were people migration into Labuhan Maringgai that cutting mangrove, there were fishermen activity in TNWK, transmigration program in Labuhan Maringgai, and land clearing into ponds in Pasir Sakti, the local government issued a regulation:Peraturan Daerah Kabupaten Lampung Timur Nomor: 03 Tahun 2002 (Rehabilitation of Coastal, Coastal and Marine in East Lampung regency), and many mangrove conservating program organized by Lampung Mangrove Center (LMC) in Labuhan Maringgai and mangrove conservator group (Mutiara Hijau I) in Pasir Sakti.
Kneser graphs are like Swiss cheese

We prove that for a large family of product graphs, and for Kneser graphs $K(n,\alpha n)$ with fixed $\alpha <1/2$, the following holds. Any set of vertices that spans a small proportion of the edges in the graph can be made independent by removing a small proportion of the vertices of the graph. This allows us to strengthen the results of [DinurFR06] and [DinurF09], and show that any independent set in these graphs is almost contained in an independent set which depends on few coordinates. Our proof is inspired by, and follows some of the main ideas of, Fox's proof of the graph removal lemma [Fox11].
A cooperative MAC protocol for wireless local area networks

In this paper, a novel idea of user cooperation in wireless networks has been exploited to improve the performance of the IEEE 802.11 medium access control (MAC) protocol. The new MAC protocol leverages the multi-rate capability of IEEE 802.11b and allows the mobile stations (STA) far away from the access point (AP) to transmit at a higher rate by using an intermediate station as a relay. Two specific variations of the new MAC protocol, namely CoopMAC I and CoopMAC II, are introduced in the paper. Both are able to increase the throughput of the whole network and reduce the average packet delay. Moreover, CoopMAC II also maintains backward compatibility with the legacy 802.11 protocol. The performance improvement is further evaluated by analysis and extensive simulations.
Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling

Cell polarity in Drosophila epithelia, oocytes and neuroblasts is controlled by the evolutionarily conserved PAR/aPKC complex, which consists of the serine-threonine protein kinase aPKC and the PDZ-domain proteins Bazooka (Baz) and PAR-6. The PAR/aPKC complex is required for the separation of apical and basolateral plasma membrane domains, for the asymmetric localization of cell fate determinants and for the proper orientation of the mitotic spindle. How the complex exerts these different functions is not known. We show that the lipid phosphatase PTEN directly binds to Baz in vitro and in vivo, and colocalizes with Baz in the apical cortex of epithelia and neuroblasts. PTEN is an important regulator of phosphoinositide turnover that antagonizes the activity of PI3-kinase. We show that Pten mutant ovaries and embryos lacking maternal and zygotic Pten function display phenotypes consistent with a function for PTEN in the organization of the actin cytoskeleton. In freshly laid eggs, the germ plasm determinants oskar mRNA and Vasa are not localized properly to the posterior cytocortex and pole cells do not form. In addition, the actin-dependent posterior movement of nuclei during early cleavage divisions does not occur and the synchrony of nuclear divisions at syncytial blastoderm stages is lost. Pten mutant embryos also show severe defects during cellularization. Our data provide evidence for a link between the PAR/aPKC complex, the actin cytoskeleton and PI3-kinase signaling mediated by PTEN.
Transcriptional activation of the tumor suppressor and differentiation gene S100A2 by a novel p63-binding site

S100A2 is generally found expressed in the epidermis and was recently shown to play a crucial role in the differentiation of keratinocytes. Also known as CaN19, S100A2 was identified as a potential tumor suppressor. Expression of S100A2 is upregulated by p53. The proteins p63 and p73 are related to p53 and are expressed as several splice variants with partially overlapping tasks but also functions different from p53. It had been shown that p63 proteins with mutations in their DNA-binding domain cause severe phenotypes in man as autosomal dominantly inherited disease including EEC, AEC, SHFM, LMS and ADULT syndromes. Here we show that S100A2 is a transcriptional target of p63/p73 family members, particularly the p63 splice variant TAp63γ. The regulation is mediated by a novel transcriptional element in the S100A2 promoter which is bound by TAp63γ but not by p53. Mutant p63 proteins derived from EEC and ADULT syndrome patients cannot activate S100A2 transcription whereas SHFM-related mutants still can stimulate the S100A2 promoter. Consistent with a function in tumor suppression S100A2 expression is stimulated upon DNA damage. After doxorubicin treatment p63γ proteins are recruited to the S100A2 promoter in vivo. This may indicate a function of the p63-dependent S100A2 regulation in tumor suppression.
NMD3 regulates both mRNA and rRNA nuclear export in African trypanosomes via an XPOI-linked pathway

Trypanosomes mostly regulate gene expression through post-transcriptional mechanisms, particularly mRNA stability. However, much mRNA degradation is cytoplasmic such that mRNA nuclear export must represent an important level of regulation. Ribosomal RNAs must also be exported from the nucleus and the trypanosome orthologue of NMD3 has been confirmed to be involved in rRNA processing and export, matching its function in other organisms. Surprisingly, we found that TbNMD3 depletion also generates mRNA accumulation of procyclin-associated genes (PAGs), these being co-transcribed by RNA polymerase I with the procyclin surface antigen genes expressed on trypanosome insect forms. By whole transcriptome RNA-seq analysis of TbNMD3-depleted cells we confirm the regulation of the PAG transcripts by TbNMD3 and using reporter constructs reveal that PAG1 regulation is mediated by its 5′UTR. Dissection of the mechanism of regulation demonstrates that it is not dependent upon translational inhibition mediated by TbNMD3 depletion nor enhanced transcription. However, depletion of the nuclear export factors XPO1 or MEX67 recapitulates the effects of TbNMD3 depletion on PAG mRNAs and mRNAs accumulated in the nucleus of TbNMD3-depleted cells. These results invoke a novel RNA regulatory mechanism involving the NMD3-dependent nuclear export of mRNA cargos, suggesting a shared platform for mRNA and rRNA export.
PHOTODYNAMIC THERAPY WITH PHOTOFRIN II INDUCES PROGRAMMED CELL DEATH IN CARCINOMA CELL LINES

Abstract The mode of cell death following photodynamic therapy was investigated from the perspective of programmed cell death or apoptosis. Human prostate carcinoma cells (PC3), human non‐small cell lung carcinoma (H322a) and rat mammary carcinoma (MTF7) were treated by photodynamic therapy. An examination of extracted cellular DNA by gel electrophoresis showed the characteristic DNA ladder indicative of internucleosomal cleavage of DNA during apoptosis. The magnitude of the response and the photodynamic therapy dosage required to induce DNA fragmentation were different in PC3 and MTF7. The MTF7 cells responded with rapid apoptosis at the dose of light and drug that yielded 50% cell death (LD50). In contrast, PC3 showed only marginal response at the LD50 but had a marked response at the LD85. Thus, apoptosis did not ensue as quickly in PC3 as in MTF7. The H322a cells were killed by photodynamic therapy but failed to exhibit any apoptotic response. The results also suggested that apoptosis in these cell lines has a minor requirement for de novo protein synthesis and no requirement for de novo RNA synthesis. This study indicates that although apoptosis can occur during photodynamic therapy‐induced cell death, this response is not universal for all cancer cell lines.
Transfusion of Red Blood Cells to Patients with Sepsis

Sepsis is one of the major causes of death worldwide, and is the host response to infection which renders our organs malfunctioning. Insufficient tissue perfusion and oxygen delivery have been implicated in the pathogenesis of sepsis-related organ dysfunction, making transfusion of packed red blood cells (pRBCs) a reasonable treatment modality. However, clinical trials have generated controversial results. Even the notion that transfused pRBCs increase the oxygen-carrying capacity of blood has been challenged. Meanwhile, during sepsis, the ability of our tissues to utilize oxygen may also be reduced, and the increased blood concentrations of lactate may be the results of strong inflammation and excessive catecholamine release, rather than impaired cell respiration. Leukodepleted pRBCs more consistently demonstrated improvement in microcirculation, and the increase in blood viscosity brought about by pRBC transfusion helps maintain functional capillary density. A restrictive strategy of pRBC transfusion is recommended in treating septic patients.
RSIM: Simulating Shared-Memory Multiprocessors with ILP Processors

The early 1990s saw several announcements of commercial shared-memory systems using processors that aggressively exploited instruction-level parallelism (ILP), including the MIPS R10000, Hewlett-Packard PA8000, and Intel Pentium Pro. These processors could potentially reduce memory read stalls by overlapping read latency with other operations, possibly changing the nature of performance bottlenecks in the system. The authors' experience with Rsim demonstrates that modeling ILP features is important even in shared-memory multiprocessor systems. In particular, current simple processor-based approximations cannot model significant performance effects for applications exhibiting parallel read misses. Further, recent shared-memory designs such as aggressive implementations of sequential consistency use the aggressive ILP-enhancing features of modern processors that simple processor-based simulators do not model. As microprocessor systems become more complex, the availability of shared infrastructure source code is likely to become increasingly crucial. The authors plan to release a new Rsim version shortly that will include instruction caches, TLBs, multimedia extensions, simultaneous multithreading, Rabbit fast simulation mode, and ports to Linux platforms.
The Time Has Come

The use of Bayesian methods for data analysis is creating a revolution in fields ranging from genetics to marketing. Yet, results of our literature review, including more than 10,000 articles published in 15 journals from January 2001 and December 2010, indicate that Bayesian approaches are essentially absent from the organizational sciences. Our article introduces organizational science researchers to Bayesian methods and describes why and how they should be used. We use multiple linear regression as the framework to offer a step-by-step demonstration, including the use of software, regarding how to implement Bayesian methods. We explain and illustrate how to determine the prior distribution, compute the posterior distribution, possibly accept the null value, and produce a write-up describing the entire Bayesian process, including graphs, results, and their interpretation. We also offer a summary of the advantages of using Bayesian analysis and examples of how specific published research based on frequentist analysis-based approaches failed to benefit from the advantages offered by a Bayesian approach and how using Bayesian analyses would have led to richer and, in some cases, different substantive conclusions. We hope that our article will serve as a catalyst for the adoption of Bayesian methods in organizational science research.
Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C4 Photosynthetic Enzyme Genes

Histone modifications play important roles in regulating the expression of C4 photosynthetic genes. Given that all enzymes required for the C4 photosynthesis pathway are present in C3 plants, it has been hypothesized that this expression regulatory mechanism has been conserved. However, the relationship between histone modification and the expression of homologs of C4 photosynthetic enzyme genes has not been well determined in C3 plants. In the present study, we cloned nine hybrid poplar (Populus simonii × Populus nigra) homologs of maize (Zea mays) C4 photosynthetic enzyme genes, carbonic anhydrase (CA), pyruvate orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxykinase (PCK), and phosphoenolpyruvate carboxylase (PEPC), and investigated the correlation between the expression levels of these genes and the levels of promoter histone acetylation modifications in four vegetative tissues. We found that poplar homologs of C4 homologous genes had tissue-dependent expression patterns that were mostly well-correlated with the level of histone acetylation modification (H3K9ac and H4K5ac) determined by chromatin immunoprecipitation assays. Treatment with the histone deacetylase inhibitor trichostatin A further confirmed the role of histone acetylation in the regulation of the nine target genes. Collectively, these results suggest that both H3K9ac and H4K5ac positively regulate the tissue-dependent expression pattern of the PsnCAs, PsnPPDKs, PsnPCKs, and PsnPEPCs genes and that this regulatory mechanism seems to be conserved among the C3 and C4 species. Our findings provide new insight that will aid efforts to modify the expression pattern of these homologs of C4 genes to engineer C4 plants from C3 plants.
Step-indexed kripke models over recursive worlds

Over the last decade, there has been extensive research on modelling challenging features in programming languages and program logics, such as higher-order store and storable resource invariants. A recent line of work has identified a common solution to some of these challenges: Kripke models over worlds that are recursively defined in a category of metric spaces. In this paper, we broaden the scope of this technique from the original domain-theoretic setting to an elementary, operational one based on step indexing. The resulting method is widely applicable and leads to simple, succinct models of complicated language features, as we demonstrate in our semantics of Charguéraud and Pottier's type-and-capability system for an ML-like higher-order language. Moreover, the method provides a high-level understanding of the essence of recent approaches based on step indexing.
Identifying Key Water Resource Vulnerabilities in Data‐Scarce Transboundary River Basins

This paper presents a two‐step framework to identify key water resource vulnerabilities in transboundary river basins where data availability on both hydrological fluxes and the operation of man‐made facilities is either limited or nonexistent. In a first step, it combines two state‐of‐the‐art modeling tools to overcome data limitations and build a model that provides a lower bound on risks estimated in that basin. Land data assimilation (process‐based hydrological modeling taking remote‐sensed products as inputs) is needed to evaluate hydrological fluxes, that is, streamflow data and consumptive use in irrigated agriculture—a lower‐end estimate of demand. Hydroeconomic modeling provides cooperative water allocation policies that reflect the best‐case management of storage capacity under hydrological uncertainty at a monthly time step for competing uses—hydropower, irrigation. In a second step, the framework uses additional scenarios to proceed with the in‐depth analysis of the vulnerabilities identified despite the use of what is by definition a best‐case model. We implement this approach to the Tigris‐Euphrates river basin, a politically unstable region where water scarcity has been hypothesized to serve as a trigger for the Syrian revolution and ensuing war. Results suggest that even under the framework's best‐case assumptions, the Euphrates part of the basin is close to a threshold where it becomes reliant on transfers of saline water from other parts of the basin to ensure irrigation demands are met. This Tigris‐Euphrates river basin application demonstrates how the proposed framework quantifies vulnerabilities that have been hitherto discussed in a mostly qualitative, speculative way.
Effectiveness of combined biliary and duodenal stenting in patients with malignant biliary and duodenal obstruction

Abstract Introduction. Concomitant biliary and duodenal obstructions are not uncommon complications in patients with gastroduodenal or pancreatobiliary malignancies. Alleviation of obstruction is very important for the palliation of inoperable patients. We studied the clinical outcomes of combined biliary and duodenal stenting. Methods. Between January 2003 and January 2010, the records of 24 patients who underwent biliary and duodenal stent placement due to inoperable malignant biliary and duodenal obstruction were reviewed retrospectively. Results. Of the 24 patients, a duodenal stent was placed after biliary stenting in 23 patients and a biliary stent was placed after duodenal stenting in one patient. Biliary stents were placed endoscopically (33 cases) or percutaneously (14 cases). Duodenal stents were placed endoscopically by fluoroscopic guidance in all patients. Oral feeding was possible at a mean of 2.7 ± 1.2 days (range, 1–6 days) after duodenal stenting. Acute pancreatitis and acute cholangitis developed in three patients and one patient, respectively, as early complications after biliary stenting. Biliary stent occlusion was developed in 12 patients and was treated successfully by stent reinsertion. As complications of duodenal stent, one case of stent migration and five cases of stent occlusion developed. Median survival after initial bilioduodenal stenting was 195.5 days (range, 21–725 days). Stent patency was well maintained in 83.3% of patients after combined stent placements while patients were alive. Conclusion. Combined biliary and duodenal stenting seems to be safe and effective in palliation of inoperable malignant biliary and duodenal obstruction.