Miracle-dz/UniFAR-FTUs huggingface dataset:
Experimental insights into the importance of ecologically dissimilar bacteria to community assembly along a salinity gradient

The response of local communities to marine-freshwater transitions and the processes that underlie community assembly are unclear, particularly with respect to bacteria that differ in their life strategies. Here, we implemented a transplant experiment where bacterioplankton from three regions of the Baltic Sea with differing salinities (∼3, 7 and 28 psu) were exposed to each other's environmental conditions. We found that habitat specialists were more abundant than generalists after exposure to salinity changes, irrespective of their origins. Most specialists that were selected following a salinity change were rare in the starting communities. Selection for generalists, however, was not specifically driven by the recruitment of either rare or abundant members, suggesting that taxon's initial abundance is minor relevant to the growth of generalists. Patterns in phylogenetic relatedness indicated that environmental filtering was the most influential assembly mechanism for specialists, whereas competitive interaction was more important for the assembly of generalists. Altogether, this study shows that large salinity changes promote the establishment of habitat specialists and that deterministic processes vary during community assembly for ecologically dissimilar taxa. We, therefore, propose that distinguishing assembly mechanisms of different community members helps understand and predict community dynamics in response to environmental change.
Anaerobic exercise and oxidative stress - Effect of the intense exercise on nitric oxide and malondialdehyde

The physical exercise is considered beneficial contributing for physical, psychological and social wellbeing and balance of the individual, being able to delay the aging process. The physical exercise unchains a physiological stress situation, to which, the sympathetic nervous system activity answers activating adaptation mechanisms. The availability of oxygen and the nitric oxide release, provide the formation of reactive oxygen species (ROS), related with the origin of cellular and tissue injuries. In order to evaluate the effect of the exercise we selected a set of sixteen healthy young individuals, voluntary, that they had constituted two distinct groups: the athlete, constituted by futsal athlete; and the control group, formed for individuals that did not practise any type of sport with regularity. The main objective of this work was to investigate if the acute and intense exercise originates, in both the groups, in the production and release of NO and in the production of free oxygen radicals. With this purpose the Wingate test is used (supramaximum anaerobic test executed in 30 seconds). After that, the concentrations of blood lactate, platelet and plasmatic nitric oxide and the plasmatic malondialdehyde (MDA) had been determined. All the determination had been made in two blood samples: one harvested before the exercise and the other 15 minutes after the Wingate test; with the exception of lactates, which was executed 5 minutes after the test. The innovation of the present study showed in the plasmatic malondialdehyde levels, which revealed in the athletes a significant reduction, in rest and after exercise, relatively to the control group. A significant reduction in the blood lactate concentration was verified in the athletes, after exercise, in relation to the control. On the other hand, the concentrations of total intra-platelet nitrites and released for the platelet, presented in the athletes a significant increase, in rest and after exercise, relatively to the control. The differences are related with the physical training, seeming to stimulate the adaptation mechanisms and the antioxidation defences of the athletes, conferring bigger cardiovascular protection and enhanced protection against physical and oxidative stress, comparatively to the individuals that did not practise sport with regularity. Thus, in young individuals, seems to us that the regular physical activity and the intense exercise develop a physiological adaptation, such that, after a maximum acute exercise, has pointers of an enhanced cardiovascular protection and against oxidative stress.
Enhancement and Field Evaluation of an Advanced Clearance Measurement System for Highway Structures, Including Generation of Clearance Diagrams

This report presents enhancements to STRUCTVIEW, a vehicle-based system for the measurement of roadway structure profiles. This second-generation system uses a scanning laser rangefinder to measure various structure and roadway features from a vehicle traveling at highway speed. Measurement capabilities include horizontal and vertical clearances, which can be used to support issuing permits based on vehicle height. The clearance dimensions extracted from STRUCTVIEW sensor data can be exported, e.g. using eXtensible Markup Language (XML), for generation of clearance diagrams.
User Acceptance of Wrist-Worn Activity Trackers Among Community-Dwelling Older Adults: Mixed Method Study

Background Wearable activity trackers are newly emerging technologies with the anticipation for successfully supporting aging-in-place. Consumer-grade wearable activity trackers are increasingly ubiquitous in the market, but the attitudes toward, as well as acceptance and voluntary use of, these trackers in older population are poorly understood. Objective The aim of this study was to assess acceptance and usage of wearable activity trackers in Canadian community-dwelling older adults, using the potentially influential factors as identified in literature and technology acceptance model. Methods A mixed methods design was used. A total of 20 older adults aged 55 years and older were recruited from Southwestern Ontario. Participants used 2 different wearable activity trackers (Xiaomi Mi Band and Microsoft Band) separately for each segment in the crossover design study for 21 days (ie, 42 days total). A questionnaire was developed to capture acceptance and experience at the end of each segment, representing 2 different devices. Semistructured interviews were conducted with 4 participants, and a content analysis was performed. Results Participants ranged in age from 55 years to 84 years (mean age: 64 years). The Mi Band gained higher levels of acceptance (16/20, 80%) compared with the Microsoft Band (10/20, 50%). The equipment characteristics dimension scored significantly higher for the Mi Band (P<.05). The amount a participant was willing to pay for the device was highly associated with technology acceptance (P<.05). Multivariate logistic regression with 3 covariates resulted in an area under the curve of 0.79. Content analysis resulted in the formation of the following main themes: (1) smartphones as facilitators of wearable activity trackers; (2) privacy is less of a concern for wearable activity trackers, (3) value proposition: self-awareness and motivation; (4) subjective norm, social support, and sense of independence; and (5) equipment characteristics matter: display, battery, comfort, and aesthetics. Conclusions Older adults were mostly accepting of wearable activity trackers, and they had a clear understanding of its value for their lives. Wearable activity trackers were uniquely considered more personal than other types of technologies, thereby the equipment characteristics including comfort, aesthetics, and price had a significant impact on the acceptance. Results indicated that privacy was less of concern for older adults, but it may have stemmed from a lack of understanding of the privacy risks and implications. These findings add to emerging research that investigates acceptance and factors that may influence acceptance of wearable activity trackers among older adults.
YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation

Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimuli can be transduced into intracellular biochemical signals through mechanosensors and intracellular mechanotransducers, such as YAP. However, the molecular mechanisms underlying mechanical regulation of OLs by YAP remain unknown. We found that OL morphology and interactions between OLs and neuronal axons were affected by knocking down YAP. Mechanical stretching of OL precursor cells induced nuclear YAP accumulation and assembly of focal adhesion, key platforms for mechanotransduction. Shear stress decreased the number of OL processes, whereas a dominant‐negative form of YAP suppressed these effects. To investigate the roles of YAP in postnatal OLs in vivo, we constructed a novel YAP knock‐in mouse and found that in vivo overexpression of YAP widely affected OL maturation. These results indicate that YAP regulates OL morphology and maturation in response to mechanical factors. GLIA 2017;65:360–374
Privacy as a Part of the Preference Structure of Users App Buying Decision

Information privacy and personal data in information systems are referred to as the ‘new oil’ of the 21st century. The mass adoption of smart mobile devices, sensor-enabled smart IoT-devices, and mobile applications provide virtually endless possibilities of gathering users’ personal information. Previous research suggests that users attribute very little monetary value to their information privacy. The current paper assumes that users are not able to monetize their value of privacy due to its abstract nature and non-transparent context. By defining privacy as a crucial product attribute of mobile applications the authors provide an approach to measure the importance of privacy as part of users’ preference structure. The results of the conducted choice-based conjoint Analysis emphasize the high relevance of privacy in users’ preference structure when downloading an app and provide an interesting contribution for theory and
SPICE modeling of nonlinear memristive behavior

The recent discovery of the ‘modern’ memristor has drawn great attention of both academia and industry. Given their favorable performance merits, memristors are expected to play a fundamental role in electronic industry. Modeling of memristive devices is essential for circuit design, and a number of Simulation Program with Integrated Circuit Emphasis (SPICE) models have already been introduced. The common problem in most models is that there is no threshold consideration; hence, only a few address the nonlinear nature of the device. This paper aims to present a SPICE implementation of a threshold‐type switching model of a voltage‐controlled memristive device that attributes the switching effect to a tunneling distance modulation. Threshold‐type switching is closer to the actual behavior of most experimentally realizable memristive systems, and our modeling approach addresses the issue of programming thresholds. Both the netlist and the simple schematic are provided, thus making it easy to comprehend and ready to be used. Compared with other modeling solutions, it involves significantly low‐complexity operation under an unlimited set of frequencies, and its simulation results are in good qualitative and quantitative agreement with the theoretical formulation. The proposed model is used to simulate an antiserial memristive switch, proving that it can be efficiently introduced in complex memristive circuits. Copyright © 2013 John Wiley & Sons, Ltd.
Encoding of Both Reaching and Grasping Kinematics in Dorsal and Ventral Premotor Cortices

Classically, it has been hypothesized that reach-to-grasp movements arise from two discrete parietofrontal cortical networks. As part of these networks, the dorsal premotor cortex (PMd) has been implicated in the control of reaching movements of the arm, whereas the ventral premotor cortex (PMv) has been associated with the control of grasping movements of the hand. Recent studies have shown that such a strict delineation of function along anatomical boundaries is unlikely, partly because reaching to different locations can alter distal hand kinematics and grasping different objects can affect kinematics of the proximal arm. Here, we used chronically implanted multielectrode arrays to record unit-spiking activity in both PMd and PMv simultaneously while rhesus macaques engaged in a reach-to-grasp task. Generalized linear models were used to predict the spiking activity of cells in both areas as a function of different kinematic parameters, as well as spike history. To account for the influence of reaching on hand kinematics and vice versa, we applied demixed principal components analysis to define kinematics synergies that maximized variance across either different object locations or grip types. We found that single cells in both PMd and PMv encode the kinematics of both reaching and grasping synergies, suggesting that this classical division of reach and grasp in PMd and PMv, respectively, does not accurately reflect the encoding preferences of cells in those areas. SIGNIFICANCE STATEMENT For reach-to-grasp movements, the dorsal premotor cortex (PMd) has been implicated in the control of reaching movements of the arm, whereas the ventral premotor cortex (PMv) has been associated with the control of grasping movements of the hand. We recorded unit-spiking activity in PMd and PMv simultaneously while macaques performed a reach-to-grasp task. We modeled the spiking activity of neurons as a function of kinematic parameters and spike history. We applied demixed principal components analysis to define kinematics synergies. We found that single units in both PMd and PMv encode the kinematics of both reaching and grasping synergies, suggesting that the division of reach and grasp in PMd and PMv, respectively, cannot be made based on their encoding properties.
Determining Experimental Parameters for Thermal-Mechanical Forming Simulation considering Martensite Formation in Austenitic Stainless Steel

The forming behavior of metastable austenitic stainless steel is mainly dominated by the temperature-dependent TRIP effect (transformation induced plasticity). Of course, the high dependency of material properties on the temperature level during forming means the temperature must be considered during the FE analysis. The strain-induced formation of α’-martensite from austenite can be represented by using finite element programs utilizing suitable models such as the Haensel-model. This paper discusses the determination of parameters for a completely thermal-mechanical forming simulation in LS-DYNA based on the material model of Haensel. The measurement of the martensite evolution in non-isothermal tensile tests was performed with metastable austenitic stainless steel EN 1.4301 at different rolling directions between 0° and 90°. This allows an estimation of the influence of the rolling direction to the martensite formation. Of specific importance is the accuracy of the martensite content measured by magnetic induction methods (Feritscope). The observation of different factors, such as stress dependence of the magnetisation, blank thickness and numerous calibration curves discloses a substantial important influence on the parameter determination for the material models. The parameters obtained for use of Haensel model and temperature-dependent friction coefficients are used to simulate forming process of a real component and to validate its implementation in the commercial code LS-DYNA.
Expression of the cancer stem cell markers ABCG2 and OCT-4 in right-sided colon cancer predicts recurrence and poor outcomes

Right-sided colon cancer (RCC) has a poorer prognosis and a higher relapse rate than left-sided colon cancer (LCC). Like cancer stem cells (CSCs), RCC cells cannot be fully eradicated and are often involved in relapse or metastasis. Because CSCs may be linked with poor outcomes, CSC markers may have prognostic value in RCC. ATP-binding cassette sub-family G member 2 (ABCG2) and OCT-4 (also known as POU5F1) are among the most useful markers for CSC identification. We therefore examined the malignant behavior of ABCG2 and OCT-4 in vitro and in vivo, and their expression was assessed in pathology tissues obtained from clinicopathologically recurrent and non-recurrent cases. Our survey suggested associations between ABCG2 and OCT-4 expression and RCC clinicopathological variables. No correlations were detected between ABCG2 or OCT-4 expression and age, gender, tumor size, or tumor shape, but ABCG2 expression correlated with TNM stage, tumor differentiation, and lymphovascular invasion. Additionally, expression of both ABCG2 and OCT-4 correlated with RCC recurrence and poor outcomes.
Triple test, a diagnostic observation, can detect cognitive impairment in older adults

A simple, quick, and efficient screening tool for detecting mild cognitive impairment (MCI) and Alzheimer’s disease (AD) is essential, especially in the primary care setting. In this study, we examined the neuropsychological profiles of elderly patients and aimed to assess the diagnostic value of the triple test, comprised of the attended alone sign (AAS), head‐turning sign, and applause sign (AS), for detecting MCI and AD.
Depressive symptoms increase the risk of progression to dementia in subjects with mild cognitive impairment: systematic review and meta‐analysis

There is a long‐standing debate in the literature whether depressive symptoms increase the risk of dementia in older with mild cognitive impairment (MCI). We aim to conduct a meta‐analysis of studies that evaluated the risk of dementia in subjects with MCI and depressive symptoms compared with subjects with MCI and no depressive symptoms.
Knockdown of Anillin Actin Binding Protein Blocks Cytokinesis in Hepatocytes and Reduces Liver Tumor Development in Mice Without Affecting Regeneration.

BACKGROUND & AIMS Cytokinesis can fail during normal postnatal liver development, leading to polyploid hepatocytes. We investigated whether inhibiting cytokinesis in the liver slows tumor growth without compromising the health of normal hepatocytes. We inhibited cytokinesis in cancer cells by knocking down ANLN, a cytoskeletal scaffolding protein that regulates cytokinesis and might promote tumorigenesis, in mice with liver disease. METHODS We analyzed clinical and gene expression data from The Cancer Genome Atlas, Oncomine, PrognoScan, and a hepatocellular carcinoma (HCC) tissue microarray. We knocked down ANLN with small interfering RNAs (siRNAs) in H2.35 liver cells and performed image analyses of cells undergoing cytokinesis. siRNAs were delivered to LAP-MYC mice, which develop hepatoblastoma, using lipid nanoparticles. H2.35 cells with knockdown of ANLN or control cells were injected into FRG mice, which develop chronic liver damage, and tumor growth was monitored. We also developed mice with inducible expression of transgenes encoding small hairpin RNAs (shRNAs) against Anln messenger RNA and studied liver tumorigenesis after administration of diethylnitrosamine and carbon tetrachloride. siRNAs against Anln messenger RNA were conjugated to N-acetylgalactosamine to reduce toxicity and increase hepatocyte tropism; their effects were studied in mouse models of liver cancer and regeneration. RESULTS Levels of ANLN messenger RNA were increased in human HCC tissues compared to non-tumor liver tissues. siRNA knockdown of ANLN blocked cytokinesis in H2.35 liver cells. Administration of siRNA against ANLN increased survival times of LAP-MYC mice, compared to mice given a control siRNA. H2.35 liver cells with shRNA knockdown of ANLN formed tumors more slowly in FRG mice than control H2.35 cells. Mice with inducible expression of shRNAs against Anln mRNA developed fewer liver tumors after administration of diethylnitrosamine and carbon tetrachloride than control mice. Knockdown of ANLN did not affect liver regeneration after acute and chronic liver injuries. CONCLUSIONS Knockdown of ANLN in liver cells blocks cytokinesis and inhibits development of liver tumors in mice. Agents that inhibit ANLN in the liver might be effective for prevention or treatment of HCC.
Electrophilic Trifluoromethylselenolation of Boronic Acids

Trifluoromethylselenylated compounds are emergent compounds with interesting physicochemical properties that still suffer from a lack of efficient synthetic methods. We recently developed an efficient one-pot strategy to generate in situ CF3SeCl and use it in various reactions. Herein, we continue our study of the reactivity scope of this preformed reagent. Cross-coupling reactions with aromatic and heteroaromatic boronic acids have been investigated. The expected products have been obtained, using a stoichiometric amount of copper, with moderate yields.
Health-related quality of life in older patients with acute coronary syndrome randomised to an invasive or conservative strategy. The After Eighty randomised controlled trial

Objective in the After Eighty study (ClinicalTrials.gov.number, NCT01255540), patients aged 80 years or more, with non-ST-elevation myocardial infarction (NSTEMI), and unstable angina pectoris (UAP), were randomised to either an invasive or conservative management approach. We sought to compare the effects of these management strategies on health related quality of life (HRQOL) after 1 year. Methods the After Eighty study was a prospective randomised controlled multicenter trial. In total, 457 patients aged 80 or over, with NSTEMI or UAP, were randomised to either an invasive strategy (n = 229, mean age: 84.7 years), involving early coronary angiography, with immediate evaluation for percutaneous coronary intervention, coronary artery bypass graft, optimal medical therapy, or to a conservative strategy (n = 228, mean age: 84.9 years). The Short Form 36 health survey (SF-36) was used to assess HRQOL at baseline, and at the 1-year follow-up. Results baseline SF-36 completion was achieved for 208 and 216 patients in the invasive and conservative groups, respectively. A total of 137 in the invasive group and 136 patients in the conservative group completed the SF-36 form at follow-up. When comparing the changes from follow-up to baseline (delta) no significant changes in quality-of-life scores were observed between the two strategies in any of the domains, expect for a small but statistically significant difference in bodily pain. This difference in only one of the SF-36 subscales may not necessarily be clinically significant. Conclusion from baseline to the 1 year follow-up, only minor differences in change of HRQOL as measured by SF-36 were seen by comparing an invasive and conservative strategy. ClinicalTrials.gov identifier NCT01255540.