Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex

Efficient transcription is linked to modification of chromatin. For instance, tri-methylation of lysine 4 on histone H3 (H3K4) strongly correlates with transcriptional activity and is regulated by the Bur1/2 kinase complex. We found that the evolutionarily conserved Ccr4-Not complex is involved in establishing H3K4 tri-methylation in Saccharomyces cerevisiae. We observed synthetic lethal interactions of Ccr4-Not components with BUR1 and BUR2. Further analysis indicated that the genes encoding the Not-proteins are essential for efficient regulation of H3K4me3, but not H3K4me1/2, H3K36me2 or H3K79me2/3 levels. Moreover, regulation of H3K4me3 levels by NOT4 is independent of defects in RNA polymerase II loading. We found NOT4 to be important for ubiquitylation of histone H2B via recruitment of the PAF complex, but not for recruitment or activation of the Bur1/2 complex. These results suggest a mechanism in which the Ccr4-Not complex functions parallel to or downstream of the Bur1/2 kinase to facilitate H3K4me3 via PAF complex recruitment.
The Prediction of Therapeutic Response to Long-Term Intensive Treatment of Seriously Disturbed Young Adult Inpatients

Most investigations of predictors of therapeutic change report that less seriously disturbed patients have greater capacity to benefit from treatment while other investigations indicate that more seriously disturbed patients have greater potential for change. The results of the present study indicate that the prognosis for seriously disturbed young adults to gain from long-term, intensive, inpatient treatment is indicated by an initial capacity to communicate disordered thinking and disruptive experiences as well as a capacity for establishing appropriate and constructive interpersonal relationships. Greater disordered thinking and the representation of more malevolent, unilateral interactions, as well as more differentiated and integrated representations of the human figure on the Rorschach early in treatment, related to less intense and frequent clinical symptoms and more intact and appropriate interpersonal relationships after approximately 15 months of treatment. While the relevance of these findings .
Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction

Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four base-long 5′-overhangs, and T4 DNA polymerase treatment of the insert in presence of a single dNTP to create vector-compatible four base-long overhangs. Notably, the insert preparation does not require any restriction enzyme treatment. The BsaI sites in the vector are oriented in such a manner that upon digestion with BsaI, a stuffer sequence along with both BsaI recognition sequences is removed. The sequence of the four base-long overhangs produced by BsaI cleavage were designed to be non-palindromic, non-compatible to each other. Therefore, only ligation of an insert carrying compatible ends allows directional cloning of the insert to the vector to generate a recombinant without recreating the BsaI sites. We also developed rapid protocols for insert preparation and cloning, by which the entire process from PCR to transformation can be completed in 6–8 h and DNA fragments ranging in size from 200 to 2200 bp can be cloned with equal efficiencies. One protocol uses a single tube for insert preparation if amplification is performed using polymerases with low 3′-exonuclease activity. The other protocol is compatible with any thermostable polymerase, including those with high 3′-exonuclease activity, and does not significantly increase the time required for cloning. The suitability of this method for high-throughput cloning was demonstrated by cloning batches of 24 PCR products with nearly 100% efficiency. The cloning strategy is also suitable for high efficiency cloning and was used to construct large libraries comprising more than 108 clones/µg vector. Additionally, based on this strategy, a variety of vectors were constructed for the expression of proteins in E. coli, enabling large number of different clones to be rapidly generated.
Human exposure in arc-welding processes: Current versus previous ICNIRP basic restrictions

This work deals with human exposure of workers to electromagnetic fields generated by manual arc-welding processes, and focuses on the comparison between current ICNIRP basic restrictions (2010), defined as internal electric field values, and previous ICNIRP basic restrictions (1998), still in force in the EU till the Directive 2004/40/EC will be revised, defined in terms of induced current density. To this aim, numerical simulation is used to mimic the exposure setup defined in Standard EN 50444, in the severe case of Tungsten Inert Gas (TIG) welding involving square-wave currents. It is shown that application of the new ICNIRP Guidelines leads to a significant reduction of the exposure index.
Human Immunodeficiency Virus (HIV) Leukoencephalopathy and the Microcirculation

We studied the brains of three patients with acquired immune deficiency syndrome (AIDS), all of whom developed subacutely progressive dementia unassociated with opportunistic infection or neoplasm in the central nervous system. Computed tomographic (CT) scans of the head revealed cortical atrophy, ventricular dilatation, and diffuse hypodensity of the centrum semiovale. On microscopic examination, the cerebral and cerebellar white matter in all cases showed diffuse and focal, angiocentric regions of myelin pallor, focal vacuolization, and extensive gliosis. Variable axonal loss and axonal spheroids were evident. The microvasculature showed striking changes, including mural thickening, increased cellularity, and enlargement and pleomorphism of endothelial cells with variable numbers of macrophages and multinucleated giant cells (MNGC), which often contained hemosiderin pigment. Human immunodeficiency virus type 1 (HIV-1) antigens were identified immunocytochemically within perivascular macrophages and MNGC and in some microglial cells. We suggest that the morphologic abnormalities of the microcirculation may be associated with an alteration of the blood-brain barrier. The increased vascular permeability could contribute to damage and loss of the white matter including both myelin and axons, and result in subcortical cerebral atrophy. The HIV-1 infected cells present in relation to the microvasculature may play a role in mediating the vascular injury.
Slim enough to swim? Weight pressures for competitive swimmers and coaching implications.

Swimmers found themselves in the spotlight at the 2000 Sydney Olympics and in the scrutiny of spectators and sport researchers alike. An attempt is often made to determine what the athlete is feeling behind the scenes and this article will focus on body weight concerns among swimmers. While sports such as gymnastics (Conviser, Fitzgibbon, & Kahn, 2000) and figure skating (Smith, 1997; Ryan, 1995) have been consistently labeled "leanness-demand" sports, the weight-related pressures for swimmers have been less clear. Benson and Taub (1993) reported that swimmers feel pressure to drop weight, "Swimmers may be especially vulnerable to disordered eating due to the display of their bodies in a tight and revealing team uniform" (p. 360).
Health and Quality of Life Outcomes Measurement Properties of the Brazilian Version of the Pediatric Quality of Life Inventory (pedsql™) Cancer Module Scale

Background: The use of health-related quality of life (HRQOL) measurements has been increased progressively in health surveys. These measurements document the functional and psychosocial outcomes of health conditions and complement clinical indicators to provide a comprehensive description of individuals and populations' health. The Pediatric Quality of Life Inventory™ (PedsQL™) is a promising instrument with age-appropriate versions. The objective of the current paper was to evaluate the psychometric properties of the PedsQL™ 3.0 Cancer Module cross-culturally adapted for use in Brazil.
International Students of Isfahan University of Medical Sciences: A Survey about Their Needs and Difficulties

Conflict of interest: none declared. Introduction International students experience difficult situations in a foreign country that may intensify other problems, which are expected in these situations and may have harmful effects on the potential function of students. As the main problems of international students may vary in different situations, the aim of this study was to investigate the main problems of international students in Iran. Methods In this cross sectional survey, all international students in Isfahan University of Medical Sciences who were in Iran during 2006-2007 were evaluated by a questionnaire consisting of six scoops of educational, psychological, supporting–communication, familial, economical and sociocultural. Results Problems referred to sociocultural scope had greater mean (2.44) followed by communication problems (2.30) and economical problems (2.16). Conclusion We suggest that consulting centers should employ experts familiar with international students problems capable of communicating well with international students.
Anti-Tumor Necrosis Factor Alpha Therapy and Periodontal Inflammation in Rheumatoid Arthritis. A clinical and biochemical approach

CODRINA ANCUTA1,2, EUGEN ANCUTA3*, RODICA CHIRIEAC4, MAGDA ANTOHE5, CRISTINA IORDACHE5 1 Grigore T. Popa University of Medicine and Pharmacy Iasi, Rheumatology Department, 16 Universitatii Str. , 700115, Iasi, Romania 2 Clinical Rehabilitation Hospital, Rheumatology 2 Department, 14 Pantelimon Halipa Str. , 700661, Iasi, Romania 3 Elena Doamna Clinical Hospital, Research Department, 49 Elena Doamna Str. , 700389, Iasi, Romania 4 SANOCARE Medical Center, 3A Ghica Voda Str. , 700400, Iasi, Romania 5 Grigore T. Popa University of Medicine and Pharmacy Iasi, Department of Implantology, Removable Dentures, Technology, 16 Universitatii Str. , 700115, Iasi, Romania
The impact of short-term professional development on participant outcomes: a review of the literature

A narrative literature review was conducted to identify the design features of effective short-term face-to-face professional development (PD) events. The 23 reviewed studies described PD with durations of 30 hours or less and involved participants in education or human service-related professions. Design features associated with positive impacts of short-term PD include sufficient time based on topic complexity, the use of learning objectives, alignment with participants’ training needs, demonstrations of desired behaviors, opportunities for participant practice, group discussions, pre-work and homework, active learning tasks that require cognitive processing, a participant-centered setting and follow-up support to promote transfer of learning.
Classroom Management: Beliefs of Preservice Teachers and Classroom Teachers Concerning Classroom Management Styles.

Noting that preservice teachers and experienced teachers share a concern for classroom management, this study compared the beliefs of classroom teachers, intern teachers, and senior level practicum students regarding classroom management styles. Participating in the study were 43 early childhood and 44 elementary education preservice teachers, and 87 classroom teachers. Data were collected by means of the Inventory of Classroom Management Style subscales to address the instructional, disciplinary, and personal dimensions of classroom management. It was hypothesized that greater experience in teaching would be associated with less interventionist management styles. Findings suggested that there were no subscale differences on the Classroom Management Style instrument. Practicum students, those with the least teaching experience, were more non-interventionist than other groups. Interns, with the middle level of teaching experience, were more interventionist than both practicum students and experienced teachers. (The framework for the inventory of classroom management style is appended.) (KB) Reproductions supplied by EDRS are the best that can be made from the original document.
Computation of Parameters of Power Transformer Windings for Use in Frequency Response Analysis

We present a 3-D model for calculating magnetic fields in a power transformer and the effective parameters (inductance and resistance) of its windings. The transformer is representative of large transformers with power ratings ranging from hundreds of kilovolt amperes to hundreds of megavolt amperes. The model accounts for anisotropic frequency-dependent properties of the laminated transformer core and eddy currents in the steel sheets. We discuss the results of the calculations performed in the frequency range 10 Hz-10 MHz, and show that the largest variations of both the magnetic field and parameters of the windings take place at frequencies below ~10 kHz and, at frequencies higher than 1 MHz, the magnetic core does not significantly affect the variation of the effective parameters
Personality factors in human deception detection: comparing human to machine performance

Previous studies of human performance in deception detection have found that humans generally are quite poor at this task, comparing unfavorably even to the performance of automated procedures. However, different scenarios and speakers may be harder or easier to judge. In this paper we compare human to machine performance detecting deception on a single corpus, the ColumbiaSRI-Colorado Corpus of deceptive speech. On average, our human judges scored worse than chance — and worse than current best machine learning performance on this corpus. However, not all judges scored poorly. Based on personality tests given before the task, we find that several personality factors appear to correlate with the ability of a judge to detect deception in speech. Index Terms: deception, deceptive, perception, personality.
Government E-Recruiting Web Sites: The Influence of E-Recruitment Content and Usability on Recruiting and Hiring Outcomes in US State Governments

This research examines both the content and usability of e-recruiting web sites on a government's ability to attract individuals to apply for government positions and on government's ability to retain new hires. Building on previous studies evaluating corporate recruiting web pages, this study evaluates the e-recruiting efforts of state governments in the United States. Our study shows that governments with more usable hiring web site receive significantly more applications per job opening; whereas states with higher quality content on their recruiting web pages have significantly less voluntary turnover of new hires.
Neurofibromin Regulates Neural Stem Cell Proliferation, Survival, and Astroglial Differentiation In Vitro and In Vivo

Neurofibromatosis 1 (NF1) is a common inherited disease in which affected children exhibit abnormalities in astrocyte growth regulation and are prone to the development of brain tumors (astrocytoma). Previous studies from our laboratory demonstrated that Nf1 mutant mouse astrocytomas contains populations of proliferating nestin+ progenitor cells, suggesting that immature astroglial progenitors may serve as a reservoir of proliferating tumor cells. Here, we directly examined the consequences of Nf1 inactivation on neural stem cell (NSC) proliferation in vitro and in vivo. We found dose-dependent effects of neurofibromin expression on NSC proliferation and survival in vitro, which reflected increased RAS pathway activation and increased bcl2 expression. In addition, unlike wild-type NSCs, Nf1-/- NSCs and, to a lesser extent, Nf1+/- NSCs survive as xenografts in naive recipient brains in vivo. Although Nf1-/- NSCs are multipotent, Nf1-/- and Nf1+/-, but not wild-type, NSCs generated increased numbers of morphologically abnormal, immature astroglial cells in vitro. Moreover, the Nf1-/- NSC growth and survival advantage as well as the astroglial cell differentiation defect were completely rescued by expression of the GAP (RAS-GTPase activating protein) domain of neurofibromin. Finally, the increase in astroglial progenitors and proliferating cells seen in vitro was also observed in Nf1-/- and Nf1+/- embryonic as well as Nf1+/- adult brains in vivo. Collectively, these findings support the hypothesis that alterations in neurofibromin expression in the developing brain have significant consequences for astrocyte growth and differentiation relevant to normal brain development and astrocytoma formation in children.
Rating systems for evaluation of the elbow.

INTRODUCTION Many scoring systems have been used for elbow disorders. However, only few of these have been validated, and many assess only few aspects of elbow function. METHODS A literature search was performed using the keyword 'elbow' in combination with 'scoring system', 'outcome assessment', 'elbow disorder' and 'clinical evaluation'. RESULTS Eighteen scoring systems are currently available for the evaluation of elbow disorders. Each of them evaluates the elbow performance using specific variables, including both objective and subjective criteria. All these scoring systems are presented. DISCUSSION Although many scoring systems have been used to evaluate elbow function, we are still far from a single outcome evaluation system which is reliable, valid and sensitive to clinically relevant changes, takes into account both patients' and physicians' perspective and is short and practical to use. CONCLUSION Further studies are required to evaluate the reliability, validity and sensitivity of the elbow scoring systems used in the common clinical practice.
The Validity of the Personal Orientation Inventory for Measuring the Effects of Training for Therapeutic Practice

The present study examines the effects of training counselors for the core therapeutic conditions of empathy, warmth, and genuineness in terms of increased levels of actualizing as measured by the Personal Orientation Inventory. Findings revealed significant differences between the experimental group and the contrast and control groups on the major Inner Directed scale and significant positive pretest to posttest changes for the experimental group on subscales of Spontaneity, Self-Regard, and Synergy. Correlations between POI scales and independent ratings of the core conditions showed that all three conditions of empathy, warmth, and genuineness were significantly related to the Inner Directed, Feeling Reactivity, and Spontaneity scales and that genuineness was also significantly correlated with the Synergy scale.
Validation of a music mood induction procedure: Some preliminary findings

Abstract Two selections of music, judged by raters to differentially affect their self-reported happiness, exhilaration, despondency, and sadness, were used as a mood induction procedure to investigate the effects of “happy” and “sad” music on behaviour and self-reported mood, and to assess the contribution of explicit demand characteristics to the procedure. The design was similar to those used by Velten (1968) and Polivy and Doyle (1980). Thirty-five subjects listened to “happy”, “sad”, or no music. Explicit instructions to subjects regarding the music were varied across groups. All subjects then completed two self-report measures and four behavioural tasks. It was found that the music mood induction procedure differentially affected self-reported mood and some measures of behavioural change, and explicit demand characteristics were found not to have contributed substantially to the mood effects found.
Prenatal exposure to common environmental factors affects brain lipids and increases risk of developing autism spectrum disorders

The prevalence of autism spectrum disorders (ASDs) has been on the rise over recent years. The presence of diverse subsets of candidate genes in each individual with an ASD and the vast variability of phenotypical differences suggest that the interference of an exogenous environmental component may greatly contribute to the development of ASDs. The lipid mediator prostaglandin E2 (PGE2) is released from phospholipids of cell membranes, and is important in brain development and function; PGE2 is involved in differentiation, synaptic plasticity and calcium regulation. The previous review already described extrinsic factors, including deficient dietary supplementation, and exposure to oxidative stress, infections and inflammation that can disrupt signaling of the PGE2 pathway and contribute to ASDs. In this review, the structure and establishment of two key protective barriers for the brain during early development are described: the blood–brain barrier; and the placental barrier. Then, the first comprehensive summary of other environmental factors, such as exposure to chemicals in air pollution, pesticides and consumer products, which can also disturb PGE2 signaling and increase the risk for developing ASDs is provided. Also, how these exogenous agents are capable of crossing the protective barriers of the brain during critical developmental periods when barrier components are still being formed is described. This review underlines the importance of avoiding or limiting exposure to these factors during vulnerable periods in development.
Preliminary Results of the Luna Aneurysm Embolization System in a Rabbit Model: A New Intrasaccular Aneurysm Occlusion Device

BACKGROUND AND PURPOSE: Recent advances in endovascular devices have been aimed at providing high density, mesh-like metallic materials across the aneurysm neck, in place of coil technology. Therefore our aim was to report the in vivo preclinical performance of a self-expanding intrasaccular embolization device. MATERIALS AND METHODS: Elastase-induced aneurysms were created in 12 rabbits. Each aneurysm was embolized with a Luna AES. DSA was performed preimplantation; 5, 10, and 30 minutes postimplantation; and at 1 month in 12 rabbits and at 3 months in 8 rabbits. Early postimplantation intra-aneurysmal flow was graded as unchanged, moderately diminished, or completely absent. One- and 3-month DSAs were graded by using a 3-point scale (complete, near-complete, or incomplete occlusion). Aneurysms were harvested for gross and microscopic histologic evaluation at 1 month (n = 4) and at 3 months (n = 8). Tissues within the aneurysm dome and across the aneurysm neck were assessed by using HE staining. RESULTS: Ten (83%) of 12 aneurysms demonstrated complete cessation of flow within 30 minutes of device implantation. At 1-month follow-up, 10 (83%) of 12 aneurysms were completely occluded. At 3 months, 7 of 8 (88%) aneurysms remained completely occluded. One-month gross examination in 4 rabbits demonstrated that membranous tissue completely covered the device in 3 subjects (75%). Microscopic examination showed that 3 aneurysms had loose connective tissue filling the aneurysm cavity. Three-month gross and microscopic examinations demonstrated membranous tissue completely covering the device, loose connective tissue filling the aneurysm cavity, and neointima formation crossing the aneurysm neck in 8 of 8 (100.0%) subjects. CONCLUSIONS: The Luna AES achieved high rates of complete angiographic occlusion and showed promising histologic findings in the rabbit aneurysm model.