Miracle-dz/UniFAR-FTUs huggingface dataset:
Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production

We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low.
Strategies in the Surgical Management of Atrial Fibrillation

Atrial fibrillation (AF) is associated with substantial morbidity, mortality, and economic burden and confers a lifetime risk of up to 25%. Current medical management involves thromboembolism prevention, rate, and rhythm control. An increased understanding of AF pathophysiology has led to enhanced pharmacological and medical therapies; however this is often limited by toxicity, variable symptom control, and inability to modulate the atrial substrate. Surgical AF ablation has been available since the original description of the Cox Maze procedure, either as a standalone or concomitant intervention. Advances in novel energy delivery systems have allowed the development of less technically demanding procedures potentially eliminating the need for median sternotomy and cardiopulmonary bypass. Variations in the definition, duration, and reporting of AF have produced methodological limitations impacting on the validity of interstudy comparisons. Standardization of these parameters may, in future, allow us to further evaluate clinical endpoints and establish the efficacy of these techniques.
The Rotator Cuff Opposes Superior Translation of the Humeral Head

To determine the influence of rotator cuff muscle activity on humeral head migration relative to the glenoid during active arm elevation we studied five fresh cadaveric shoulders. The shoulder girdles were mounted in an apparatus that simulated contraction of the deltoid and rotator cuff muscles while maintaining the normal scapulothoracic relationship. The arms were abducted using four different configurations of simulated muscle activity: deltoid alone; deltoid and supraspinatus; del toid, infraspinatus, teres minor, and subscapularis; and deltoid, supraspinatus, infraspinatus, teres minor, and subscapularis. For each simulated muscle configura tion the vertical position of the humeral head in relation to the glenoid was determined at 30°, 60°, 90°, and 120° of abduction using digitized anteroposterior radio graphs. Both muscle activity and abduction angle sig nificantly influenced the glenohumeral relationship. With simulated activity of the entire rotator cuff, the geo metric center of the humeral head was centered in the glenoid at 30° but had moved 1.5 mm superiorly by 120°. Abduction without the subscapularis, infraspina tus, and teres minor muscles caused significant supe riorly directed shifts in humeral head position as did ab duction using only the deltoid muscle. These results support the possible use of selective strengthening ex ercises for the infraspinatus, teres minor, and sub scapularis muscles in treatment of the impingement syndrome.
Clinical comparison of three methods to measure blood pressure in nonsedated dogs.

OBJECTIVE To determine arterial blood pressure in client-owned dogs, using direct arterial puncture, oscillometry, and Doppler ultrasonography in a clinical setting. DESIGN Prospective study. ANIMALS 8 clinically normal client-owned dogs. PROCEDURE Blood pressures of nonsedated dogs were measured simultaneously, using each of the 3 methods. Mean values obtained were compared with published mean values. Ability of noninvasive methods (Doppler ultrasonography and oscillometry) to accurately predict results of the invasive method, and relationships between blood pressure and age, body weight, and degree of patient anxiety were determined. RESULTS Calculated ranges of values (mean +/- 2 SD) determined by direct arterial puncture were: systolic pressure, 114 to 194 mm Hg; diastolic pressure, 66 to 102 mm Hg; and mean pressure, 85 to 129 mm Hg. Ranges determined by use of oscillometry were: systolic, 110 to 190 mm Hg; diastolic, 35 to 107 mm Hg; and mean, 78 to 138 mm Hg. Ultrasonographic and oscillometric values did not accurately predict direct values, but mean values of systolic and mean pressures were similar among methods. Relationships were not detected between age or body weight and blood pressure. Significant differences in blood pressure were not detected between anxious and nonanxious dogs. CONCLUSIONS AND CLINICAL RELEVANCE Mean values of systolic, diastolic, and mean arterial blood pressure measured in nonsedated client-owned dogs, using invasive and noninvasive methods in a clinical setting, are comparable with those determined for acclimatized, trained, or sedated dogs. However, results of noninvasive methods may not accurately reflect direct values.
Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Stem rust is one of the most potentially harmful wheat diseases, but has been effectively controlled in China since 1970s. However, the interest in breeding wheat with durable resistance to stem rust has been renewed with the emergence of Ug99 (TTKSK) virulent to the widely used resistance gene Sr31, and by which the wheat stem rust was controlled for 40 years in wheat production area worldwide. Yunnan Province, located on the Southwest border of China, is one of the main wheat growing regions, playing a pivotal role in the wheat stem rust epidemic in China. This study investigated the levels of resistance in key wheat cultivars (lines) of Yunnan Province. In addition, the existence of Sr25, Sr26, Sr28, Sr31, Sr32, and Sr38 genes in 119 wheat cultivars was assessed using specific DNA markers. The results indicated that 77 (64.7%) tested wheat varieties showed different levels of resistance to all the tested races of Puccinia graminis f. sp. tritici. Using molecular markers, we identified the resistance gene Sr31 in 43 samples; Sr38 in 10 samples; Sr28 in 12 samples, and one sample which was resistant against Ug99 (avirulent to Sr32). No Sr25 or Sr26 (effective against Ug99) was identified in any cultivars tested. Furthermore, 5 out of 119 cultivars tested carried both Sr31 and Sr38 and eight contained both Sr31 and Sr28. The results enable the development of appropriate strategies to breed varieties resistant to stem rust.
Water demand management research: A psychological perspective

The availability of fresh water for human consumption is a critical global issue and one that will be exacerbated by the impacts of climate change. Water demand management has an important role to play in reducing the vulnerability of freshwater supplies to climate change impacts. In this paper, we argue that the field of psychology and environmental psychology in particular can make a vital contribution in understanding further the drivers of residential water demand. A growing body of literature in environmental psychology has examined the determinants of water conservation behavior, and this research has many potential applications for water demand policy. In this paper we offer a review of current psychological research that examines the five broad causes of residential water conservation behaviors: attitudes, beliefs, habits or routines, personal capabilities, and contextual factors. We assess how psychologists have studied water conservation behavior to date, identify shortcomings, and indicate how this research can be used to further promote residential water conservation and to inform evidence‐based policy and practice.
The Bridle Procedure in the Treatment of Paralysis of the Foot*

The Bridle procedure consists of a posterior tibial tendon transfer through the interosseous membrane to the dorsum of the foot with a dual anastomosis to the tendon of the anterior tibial and a rerouted peroneus longus in front of the lateral malleolus. This procedure is performed to restore loss of dorsiflexion and to balance the foot, preventing a secondary varus or valgus deformity. The procedure was performed in 10 patients with 11 feet with paralysis of dorsiflexion of the foot. All are brace-free. The results are better in patients with peroneal nerve palsy secondary to trauma.
A Pharmacokinetics, Efficacy, and Safety Study of Gadoterate Meglumine in Pediatric Subjects Aged Younger Than 2 Years

Objectives The primary objective of this study was to investigate the pharmacokinetic profile of gadoterate meglumine in pediatric patients younger than 2 years; the secondary objectives were to document its efficacy and safety. Material and Methods This was a Phase IV open-label, prospective study conducted in 9 centers (4 countries). Forty-five patients younger than 2 years with normal estimated glomerular filtration rate and scheduled to undergo routine gadolinium-enhanced magnetic resonance imaging (MRI) of any organ were included and received a single intravenous injection of gadoterate meglumine (0.1 mmol/kg). To perform the population pharmacokinetics analysis, 3 blood samples per subject were drawn during 3 time windows at time points allocated by randomization. Results Gadoterate meglumine concentrations were best fitted using a 2-compartmental model with linear elimination from central compartment. The median total clearance adjusted to body weight was estimated at 0.06 L/h per kg and increased with estimated glomerular filtration rate according to a power model. The median volume of distribution at steady state (Vss) adjusted to body weight was estimated at 0.047 L/kg. Estimated median terminal half-life (t1/2&bgr;) was 1.35 h, and the median systemic exposure (area under the curve) was 1591 &mgr;mol h/L. Efficacy was assessed by comparing precontrast +postcontrast images to precontrast images in a subset of 28 subjects who underwent an MRI examination of brain, spine, and associated tissues. A total of 28 lesions were identified and analyzed in 15 subjects with precontrast images versus 30 lesions in 16 subjects with precontrast + postcontrast images. Lesion visualization was improved with a mean (SD) increase in scores at subject level of 0.7 (1.0) for lesion border delineation, 0.9 (1.6) for internal morphology, and 3.1 (3.2) for contrast enhancement. Twenty-six adverse events occurred postinjection in 13 subjects (28.9%), including 3 serious reported in 1 subject (2.2%). One subject (2.2%) experienced 1 rash of moderate intensity considered as related to gadoterate meglumine. Conclusions The pharmacokinetic profile of gadoterate meglumine after a single intravenous injection of 0.1 mmol/kg was appropriately described in newborns and infants younger than 2 years, for whom no dose adjustment is required. The improved efficacy of gadoterate meglumine for contrast-enhanced MRI examination of brain, spine, and associated tissues, as well as its good safety profile, was also demonstrated in this population.
Effect of Lake Trophic Status and Rooted Macrophytes on Community Composition and Abundance of Ammonia-Oxidizing Prokaryotes in Freshwater Sediments

ABSTRACT Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland pools. Archaeal and bacterial ammonia monooxygenase alpha-subunit (amoA) gene diversity increased from oligotrophic to mesotrophic sites; the number of detected operational taxonomic units was positively correlated to ammonia availability and pH and negatively correlated to sediment C/N ratios. AOA communities could be grouped according to lake trophic status and pH; plant species-specific communities were not detected, and no grouping was apparent for AOB communities. Relative abundance, determined by quantitative PCR targeting amoA, was always low for AOB (<0.05% of all prokaryotes) and slightly higher for AOA in unvegetated sediment and AOA in association with M. alterniflorum (0.01 to 2%), while AOA accounted for up to 5% in the rhizospheres of L. uniflora and J. bulbosus. These results indicate that (i) AOA are at least as numerous as AOB in freshwater sediments, (ii) aquatic macrophytes with substantial release of oxygen and organic carbon into their rhizospheres, like L. uniflora and J. bulbosus, increase AOA abundance; and (iii) AOA community composition is generally determined by lake trophy, not by plant species-specific interactions.
Deposition of Particulate from Coal-Derived Syngas on Gas Turbine Blades Near Film Cooling Holes

DEPOSITION OF PARTICULATE FROM COAL-DERIVED SYNGAS ON GAS TURBINE BLADES NEAR FILM COOLING HOLES Weiguo Ai Department of Chemical Engineering Doctor of Philosophy Synfuel from gasification of coal, biomass, and/or petroleum coke is an alternative to natural gas in land-based industrial gas turbines. However, carryover fine particulate in the syngas may lead to a considerable amount of deposition on turbine blades, which reduces component life and system performance. Deposition experiments on film-cooled turbine components were performed in an accelerated test facility to examine the nature of flyash deposits near film cooling holes. Experimental results indicate that deposition capture efficiency decreased with increased blowing ratio. Shaped holes exhibited more span-wise coverage than cylindrical holes and effectively reduced deposition. The TBC layer increased surface temperature, resulting in increased deposition. Coupons with close hole spacing exhibited a more uniform low temperature region downstream and less deposition. Capture efficiencies for small particles were lower than for large particles, especially at low blowing ratios. The trench increased cooling effectiveness downstream, but did not reduce overall collection efficiency of particulates because the trench also acted as a particulate collector. In the numerical computations using a CFD code (FLUENT), the standard k-ω turbulence model and RANS were employed to compute flow field and heat transfer. A Lagrangian particle method was utilized to predict the ash particulate transport. Userdefined subroutines were developed to describe and predict particle deposition rates on the turbine blade surface. Small particles have a greater tendency to stick to the surface. As the surface temperature rose above the transition temperature, large particles dominated the excessive deposition due to the high delivery rate. Backside impingement of coolant improved the overall cooling effectiveness. Experiments and CFD modeling results suggest that clean coolant dominated the initial deposition process by blowing off the particles and preventing particles from impacting on the surface. Initial deposits formed between coolant channels. Subsequent deposition occurred on top of initial deposits, due to increasing deposit surface temperature, which led to the formation of distinct ridges between coolant paths.
A pediatric intramedullary spinal cord tumor with unusual solid‐cystic and papillary features: A case report

Spinal cord tumors are rare in children. We report a novel case of pediatric intramedullary spinal cord tumor with unusual solid‐cystic and papillary features. Clinically, the patient presented at the age of 3 years with motor deficit and urinary incontinence, and MRI demonstrated multilocular cystic lesions in the thoracic spine. Histologically the tumor consisted of solid, sheet‐like components and branching papillary structures, and immunohistochemistry demonstrated positive reactivity for epithelial membrane antigen, cytokeratins (7, AE1/3, CAM5.2), E‐cadherin and transthyretin, and negativity for GFAP, S‐100 protein, synaptophysin and neurofilament. These histological and immunohistochemical findings appeared to be unique, and were not compatible with the features of classical ependymoma or choroid plexus papilloma. The clinical behavior, characterized by relatively rapid tumor regrowth after surgical resection and a relatively high MIB‐1 labeling index, suggest that this tumor might have had moderate malignant potential. This pediatric case appears to be particularly informative with regard to the tumor biology or tumorigenesis of intramedullary spinal cord tumor with unusual solid‐cystic and papillary features.
The effects of feeding sericea lespedeza hay on growth rate of goats naturally infected with gastrointestinal nematodes.

Goat production is increasing in the United States due to high ethnic demand, but infection with gastrointestinal nematode (GIN) parasites is a major constraint to the industry. Increasing GIN resistance to chemical anthelmintics worldwide has led to the development of alternative control strategies, including use of forages containing condensed tannins (CT). An experiment was designed using infected and dewormed male kids (Kiko x Spanish, 6 mo old, 18.9 +/- 3.25 kg) fed diets containing 25% concentrate and either 75% sericea lespedeza [SL; Lespedeza cuneata (Dum-Cours.) G. Don], a high CT forage (87 to 181 g of CT/kg), or 75% bermudagrass [BG; Cynodon dactylon (L.) Pers. ] hay (n = 10/treatment). The kids were weighed every 14 d, and fecal and blood samples were taken weekly for fecal egg counts and packed cell volume determination, respectively. Fecal cultures were processed every 14 d to determine CT effect on larval development. At slaughter, adult GIN were collected from the abomasum and small intestines for counting and speciation. Blood samples were also analyzed for plasma urea-N, and ruminal VFA and pH were determined. The infected SL-fed kids had consistently lower (P < 0.05) fecal egg counts than the infected BG goats throughout the trial and greater (P < 0.05) packed cell volume beginning by d 77. Average daily gain was greater (P < 0.001) in kids fed SL- than BG-based diets, regardless of infection status (104.3 +/- 5.0 and 75.5 +/- 4.8 g/d, respectively). Total VFA and acetate concentrations were greater (P < 0.001) in the BG- than in SL-fed goats, whereas propionate levels were unaffected by diet. Acetate:propionate ratio (P = 0.01) and plasma urea-N (P = 0.03) levels were greater in BG-fed goats, whereas rumen pH was greater (P < 0.001) in the SL-fed goats. Feeding SL hay can reduce GIN infection levels and increase performance of goats compared with BG hay.
Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton[W][OA]

The visualization of storage, membrane, and signaling lipid species in embryos of cottonseeds at cellular resolution suggests that lipid species, even those in the same class, are distributed heterogeneously in tissues. This work provides new information about metabolite distribution and points to a previously unknown complexity in cellular biochemistry within plant tissues. Advances in mass spectrometry (MS) have made comprehensive lipidomics analysis of complex tissues relatively commonplace. These compositional analyses, although able to resolve hundreds of molecular species of lipids in single extracts, lose the original cellular context from which these lipids are derived. Recently, high-resolution MS of individual lipid droplets from seed tissues indicated organelle-to-organelle variation in lipid composition, suggesting that heterogeneity of lipid distributions at the cellular level may be prevalent. Here, we employed matrix-assisted laser desorption/ionization–MS imaging (MALDI-MSI) approaches to visualize lipid species directly in seed tissues of upland cotton (Gossypium hirsutum). MS imaging of cryosections of mature cotton embryos revealed a distinct, heterogeneous distribution of molecular species of triacylglycerols and phosphatidylcholines, the major storage and membrane lipid classes in cotton embryos. Other lipids were imaged, including phosphatidylethanolamines, phosphatidic acids, sterols, and gossypol, indicating the broad range of metabolites and applications for this chemical visualization approach. We conclude that comprehensive lipidomics images generated by MALDI-MSI report accurate, relative amounts of lipid species in plant tissues and reveal previously unseen differences in spatial distributions providing for a new level of understanding in cellular biochemistry.
Changes in surface elevation and extent of the Kaskawulsh Glacier, Yukon Territory

Between 1977 and 2007, the Kaskawulsh Glacier underwent an overall decrease in volume of between 2.76 km water equivalent (we) and 4.60 km we, and a decrease in area of 2.27%. Volume losses are consistent with changes observed at most glaciers in the Yukon/Alaska region which are thinning and/or retreating (Arendt et al. 2006; Chen et al. 2006a; Larsen et al. 2007; Molnia 2007). The rate of volume change over the periods 19771995 and 1995-2007 remained constant at -0.52 km yr" we, while between 2000 and 2007 the glacier gained volume at a rate of 1.04 km yr" we. Gains in the recent 2000-2007 period result from prominent thickening in the accumulation area above 1989 m (+16.9 m) and minor thickening in the ablation area (+0.5 m). The observed thickening pattern is similar to patterns observed in Greenland (Chen et al. 2006b; Johannessen et al. 2005; Luthcke et al. 2008b), Antarctica (Davis et al. 2005), the Karakoram Himalaya (Hewitt 2005), New Zealand (Chinn 1999; Hooker and Fitzharris 1999), Scandinavia (Bamber et al. 2004; Chinn et al. 2005, Nesje et al. 2000), and Alaska (Arendt et al. 2008; Muskett et al. 2003). Between 1956 and 2007, the glacier terminus retreated by an average of 655 m (13 m yr" ). No scaling ratio between terminus retreat and volume change could be established for the Kaskawulsh Glacier for the period 1977-2007.
Horn Fragments of the Halpern-Shoham Interval Temporal Logic

We investigate the satisfiability problem for Horn fragments of the Halpern-Shoham interval temporal logic depending on the type (box or diamond) of the interval modal operators, the type of the underlying linear order (discrete or dense), and the type of semantics for the interval relations (reflexive or irreflexive). For example, we show that satisfiability of Horn formulas with diamonds is undecidable for any type of linear orders and semantics. On the contrary, satisfiability of Horn formulas with boxes is tractable over both discrete and dense orders under the reflexive semantics and over dense orders under the irreflexive semantics but becomes undecidable over discrete orders under the irreflexive semantics. Satisfiability of binary Horn formulas with both boxes and diamonds is always undecidable under the irreflexive semantics.