Miracle-dz/UniFAR-FTUs huggingface dataset:
Possible interactions between angiotensin II and insulin: effects on glucose and lipid metabolism in vivo and in vitro.

Angiotensin II (ANGII) increases insulin sensitivity in diabetic and non-diabetic subjects, even at subpressor doses, and because there is 'crosstalk' between ANGII and insulin-signaling pathways the underlying mechanism may not be due solely to changes in regional blood flow. A series of experimental studies was undertaken to evaluate the effects of ANGII on glucose and lipid metabolism in vivo and in vitro. Groups of fructose-fed, insulin-resistant Sprague-Dawley (SD) rats were pre-treated with 0.3 mg/kg per day of the AT(1)-receptor antagonist L-158 809 (n=16), or vehicle (n=16), by oral gavage. This was prior to an oral glucose tolerance test (day 5) and measurement of the effects of ANGII infusion (20 ng/kg per min i.v. for 3 h) on whole-body insulin sensitivity using the insulin suppression test (day 7). The effect of ANGII infusion on total triglyceride secretion rate (TGSR) was evaluated in normal SD rats pretreated for 7 days with L-158 809 (n=12) or vehicle (n=12). AT(1)- and AT(2)- receptor mRNA expression and [(3)H]2-deoxyglucose uptake were assessed in cultured L6 myoblasts. Short-term treatment with L-158 809 had no effect on glucose tolerance or fasting triglyceride levels in fructose-fed rats. ANGII infusion had no effect on insulin sensitivity in fructose-fed rats pretreated with vehicle (steady-state plasma glucose (SSPG) values 8.1+/-1.6 vs 8. 4+/-0.4 mmol/l), but pretreatment with L-158 809 resulted in ANGII having a modest insulin antagonist effect in this insulin-resistant model (SSPG values 9.6+/-0.3 vs 7.1+/-0.6, P<0.03). ANGII infusion had no significant effect on TGSR (e.g. 24.6+/-1.4 vs 28.4+/-0.9 mg/100 g per h in vehicle-treated animals). RT-PCR analysis showed that L6 cells express both AT(1)- and AT(2)-receptor mRNA. Incubation with ANGII (10(-9) and 10(-8) M) had no significant effect on the dose-response curve for insulin-stimulated [(3)H]2-deoxyglucose uptake. For example, C(I200) values (dose of insulin required to increase glucose uptake by 200%) were 4.5 x 10(-9) M (control) vs 3.9 x 10(-9) M and 6.2 x 10(-9) M, whereas the positive control (glucagon-like peptide-1) increased insulin sensitivity. Thus, ANGII infusion may have a modest insulin antagonist effect on glucose disposal in insulin-resistant fructose-fed rats pretreated with an AT(1)-blocker, but ANGII has no effect on TGSR or in vitro glucose uptake in L6 myoblasts. These findings are relevant to recent clinical discussions about the metabolic effects of ANGII and renin-angiotensin system blockade.
Characterization of Substrate Preference for Slc1p and Cst26p in Saccharomyces cerevisiae Using Lipidomic Approaches and an LPAAT Activity Assay

Background Phosphatidic acid (PA) is a key regulated intermediate and precursor for de novo biosynthesis of all glycerophospholipids. PA can be synthesized through the acylation of lysophosphatidic acid (LPA) by 1-acyl-3-phosphate acyltransferase (also called lysophosphatidic acid acyltransferase, LPAAT). Recent findings have substantiated the essential roles of acyltransferases in various biological functions. Methodologies/Principal Findings We used a flow-injection-based lipidomic approach with ∼200 multiple reaction monitoring (MRM) transitions to pre-screen fatty acyl composition of phospholipids in the yeast Saccharomyces cerevisiae mutants. Dramatic changes were observed in fatty acyl composition in some yeast mutants including Slc1p, a well-characterized LPAAT, and Cst26p, a recently characterized phosphatidylinositol stearoyl incorporating 1 protein and putative LPAAT in S. cerevisiae. A comprehensive high-performance liquid chromatography–based multi-stage MRM approach (more than 500 MRM transitions) was developed and further applied to quantify individual phospholipids in both strains to confirm these changes. Our data suggest potential fatty acyl substrates as well as fatty acyls that compensate for defects in both Cst26p and Slc1p mutants. These results were consistent with those from a non-radioactive LPAAT enzymatic assay using C17-LPA and acyl-CoA donors as substrates. Conclusions We found that Slc1p utilized fatty acid (FA) 18:1 and FA 14:0 as substrates to synthesize corresponding PAs; moreover, it was probably the only acyltransferase responsible for acylation of saturated short-chain fatty acyls (12:0 and 10:0) in S. cerevisiae. We also identified FA 18:0, FA 16:0, FA 14:0 and exogenous FA 17:0 as preferred substrates for Cst26p because transformation with a GFP-tagged CST26 restored the phospholipid profile of a CST26 mutant. Our current findings expand the enzymes and existing scope of acyl-CoA donors for glycerophospholipid biosynthesis.
Aldosterone Enhances Ischemia-Induced Neovascularization Through Angiotensin II–Dependent Pathway

Background—We analyzed the role of aldosterone in ischemia-induced neovascularization and the involvement of angiotensin II (Ang II) signaling in this effect. Methods and Results—Ischemia was induced by right femoral artery ligature in mice treated or not with aldosterone (4.5 μg/day), aldosterone plus spironolactone (aldosterone receptor blocker; 20 mg/kg per day), or aldosterone plus valsartan (angiotensin type 1 [AT1] receptor blocker; 20 mg/kg per day). After 21 days, neovascularization was evaluated by microangiography, capillary density measurement, and laser-Doppler perfusion imaging. Protein level of vascular endothelial growth factor (VEGF) was determined by Western blot analysis in hindlimbs. mRNA levels of renin–angiotensin system components were also assessed by semiquantitative reverse transcription–polymerase chain reaction. Angiographic score, capillary number, and foot perfusion were improved in ischemic/nonischemic leg ratio by 1.4-, 1.5-, and 1.4-fold, respectively, in aldosterone-treated mice compared with controls (P <0.05). Aldosterone proangiogenic effect was associated with 2.3-fold increase in VEGF protein content (P <0.05). Treatments with spironolactone or with neutralizing VEGF antibody hampered the proangiogenic effect of aldosterone (P <0.05 versus aldosterone-treated mice). Interestingly, AT1 receptor blockade completely abrogated the aldosterone proangiogenic effect, emphasizing the involvement of Ang II–related pathway in aldosterone-induced vessel growth. In this view, angiotensinogen mRNA content was 2.2-fold increased in aldosterone-treated mice in reference to controls (P <0.05), whereas that of renin, angiotensin-converting enzyme, and AT1 receptor subtype was unaffected. Aldosterone treatment also decreased AT2 mRNA content by 2-fold (P <0.05 versus controls), suggesting that aldosterone may switch the Ang II pathway toward activation of vessel growth. Conclusions—This study shows for the first time that aldosterone increases neovascularization in the setting of ischemia through activation of Ang II signaling.
Prenatal detection of extra structurally abnormal chromosomes (ESACs): new cases and a review of the literature

We present 16 cases, 10 de novo and 6 familial, in which extra structurally abnormal chromosomes (ESACs) were diagnosed prenatally and identified by fluorescence in situ hybridization (FISH) studies with follow up from birth. We review the literature on prenatally diagnosed ESACs arising de novo and suggest a management protocol for these cases. Copyright © 1999 John Wiley & Sons, Ltd.
Diversity Surveys and Evolutionary Relationships of aoxB Genes in Aerobic Arsenite-Oxidizing Bacteria

ABSTRACT A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.
Melt Processed PCL/PEG Scaffold with Discrete Pore Size Gradient for Selective Cellular Infiltration

In order to develop scaffold able to mimic the natural gradient properties of tissues, biphasic and triphasic approaches were adopted. In this work, polycaprolactone/polyethylene glycol (PCL/PEG) scaffolds were prepared by using a combination of melt mixing and selective leaching without harmful solvents. The method permitted to develop three-layer scaffolds with high control of porosity and pore size. The mechanical properties were evaluated under physiological condition in order to simulate the real conditions of work. Co-culture of osteoblastic and fibroblastic mice cells were carried out in order to study the differential cellular permeation through the different pore size layers.
Word Graphs for Statistical Machine Translation

Word graphs have various applications in the field of machine translation. Therefore it is important for machine translation systems to produce compact word graphs of high quality. We will describe the generation of word graphs for state of the art phrase-based statistical machine translation. We will use these word graph to provide an analysis of the search process. We will evaluate the quality of the word graphs using the well-known graph word error rate. Additionally, we introduce the two novel graph-to-string criteria: the position-independent graph word error rate and the graph BLEU score. Experimental results are presented for two Chinese--English tasks: the small IWSLT task and the NIST large data track task. For both tasks, we achieve significant reductions of the graph error rate already with compact word graphs.
Metronidazole-Induced and Wernicke Encephalopathy: Two Different Entities Sharing the Same Metabolic Pathway?

We read with interest the recent article by Kim et al[1][1] on MR imaging features of metronidazole-induced encephalopathy (MIE). They described MR findings from 7 patients treated with metronidazole, 5 of whom suffered from gastrointestinal tract disease. All of the patients showed symmetric signal
Supercritical multitype branching processes: the ancestral types of typical individuals

For supercritical multitype Markov branching processes in continuous time, we investigate the evolution of types along those lineages that survive up to some time t. We establish almost-sure convergence theorems for both time and population averages of ancestral types (conditioned on nonextinction), and identify the mutation process describing the type evolution along typical lineages. An important tool is a representation of the family tree in terms of a suitable size-biased tree with trunk. As a by-product, this representation allows a ‘conceptual proof’ (in the sense of Kurtz et al.) of the continuous-time version of the Kesten-Stigum theorem.
Molecular Cancer Gene Expression Profiling by Dna Microarray Analysis in Mouse Embryonic Fibroblasts Transformed by Ras V12 Mutated Protein and the E1a Oncogene

Background: Ras is an area of intensive biochemical and genetic studies and characterizing downstream components that relay ras-induced signals is clearly important. We used a systematic approach, based on DNA microarray technology to establish a first catalog of genes whose expression is altered by ras and, as such, potentially involved in the regulation of cell growth and transformation.
Overview of freshwater microbial eukaryotes diversity: a first analysis of publicly available metabarcoding data.

Although they are widespread, diverse and involved in biogeochemical cycles, microbial eukaryotes attract less attention than their prokaryotic counterparts in environmental microbiology. In this study, we used publicly available 18S barcoding data to define biases that may limit such analyses and to gain an overview of the planktonic microbial eukaryotic diversity in freshwater ecosystems. The richness of the microbial eukaryotes was estimated to 100 798 operational taxonomic units (OTUs) delineating 1267 clusters or phylogenetic units (PUs, i.e. monophyletic groups of OTUs that are phylogenetically close). By summing the richness found in aquatic environments, we can predict the microbial eukaryotic richness to be around 200 000-250 000 species. The molecular diversity of protists in freshwater environments is generally higher than that of the morphospecies and cultivated species catalogued in public databases. Amoebozoa, Viridiplantae, Ichthyosporea, and Cryptophyta are the most phylogenetically diverse taxa, and characterisation of these groups is still needed. A network analysis showed that Fungi, Stramenopiles and Viridiplantae play central role in lake ecosystems. Finally, this work provides guidance for compiling metabarcoding data and identifies missing data that should be obtained to increase our knowledge on microbial eukaryote diversity.
The Exact Complexity of the First-Order Logic Definability Problem

We study the definability problem for first-order logic, denoted by FO-Def. The input of FO-Def is a relational database instance I and a relation R; the question to answer is whether there exists a first-order query Q (or, equivalently, a relational algebra expression Q) such that Q evaluated on I gives R as an answer. Although the study of FO-Def dates back to 1978, when the decidability of this problem was shown, the exact complexity of FO-Def remains as a fundamental open problem. In this article, we provide a polynomial-time algorithm for solving FO-Def that uses calls to a graph-isomorphism subroutine (or oracle). As a consequence, the first-order definability problem is found to be complete for the class GI of all problems that are polynomial-time Turing reducible to the graph isomorphism problem, thus closing the open question about the exact complexity of this problem. The technique used is also applied to a generalized version of the problem that accepts a finite set of relation pairs, and whose exact complexity was also open; this version is also found to be GI-complete.
Robust text-independent speaker identification over telephone channels

This paper addresses the issue of closed-set text-independent speaker identification from samples of speech recorded over the telephone. It focuses on the effects of acoustic mismatches between training and testing data, and concentrates on two approaches: (1) extracting features that are robust against channel variations and (2) transforming the speaker models to compensate for channel effects. First, an experimental study shows that optimizing the front end processing of the speech signal can significantly improve speaker recognition performance. A new filterbank design is introduced to improve the robustness of the speech spectrum computation in the front-end unit. Next, a new feature based on spectral slopes is described. Its ability to discriminate between speakers is shown to be superior to that of the traditional cepstrum. This feature can be used alone or combined with the cepstrum. The second part of the paper presents two model transformation methods that further reduce channel effects. These methods make use of a locally collected stereo database to estimate a speaker-independent variance transformation for each speech feature used by the classifier. The transformations constructed on this stereo database can then be applied to speaker models derived from other databases. Combined, the methods developed in this paper resulted in a 38% relative improvement on the closed-set 30-s training 5-s testing condition of the NIST'95 Evaluation task, after cepstral mean removal.
Identification of Streptococcus sanguinis with a PCR-Generated Species-Specific DNA Probe

ABSTRACT The objective of the present study was to design a PCR-generated DNA probe and determine the specificity of the probe for the identification of clinical isolates of Streptococcus sanguinis. To do this, we examined over 200 arbitrarily primed PCR (AP-PCR) amplicon patterns obtained with DNA from clinical isolates of S. sanguinis. A 1.6-kb DNA amplicon that was common to all AP-PCR profiles was extracted from agarose gels and then cloned and sequenced. A search for a similar sequence in the GenBank database with the BLASTN program revealed that the 1.6-kb DNA fragment comprised an intergenic region between two housekeeping genes, uncC (proton-translocating ATPase) and murA (UDP-N-acetylglucosamine enolpyruvyl transferase). Three digoxigenin-labeled DNA probes were synthesized on the basis of the sequence of the 1.6-kb fragment: the sequence of probe SSA-1 contained the proton-translocating ATPase (uncC) and the entire intergenic region, the sequence of probe SSA-2 contained only the intergenic region, and the sequence of probe SSA-3 contained an internal region of the murA gene. Dot blot hybridization showed that the three probes displayed signals for hybridization to both S. sanguinis strain ATCC 10556 and the S. sanguinis clinical isolates. Probe SSA-1, however, hybridized to DNA from S. oralis and S. mitis. Probe SSA-3 hybridized to DNA from S. gordonii, S. mitis, S. oralis, S. parasanguinis, and S. vestibularis. The probe SSA-2-specific intergenic region appeared to be specific for S. sanguinis. The results from this study suggest that probe SSA-2 may serve as a species-specific DNA probe for the identification of clinical isolates of S. sanguinis.
Maximum Likelihood Fitting of Tidal Streams with Application to the Sagittarius Dwarf Tidal Tails

We present a maximum likelihood method for determining the spatial properties of tidal debris and of the Galactic spheroid. With this method we characterize Sagittarius debris using stars with the colors of blue F turnoff stars in SDSS stripe 82. The debris is located at (α ,δ ,R) = (31.37°± 0.26°,0.0°,29.22 ± 0.20 kpc) , with a (spatial) direction given by the unit vector (− 0.991 ± 0.007 kpc ,0.042 ± 0.033 kpc ,0.127 ± 0.046 kpc) , in galactocentric Cartesian coordinates, and with FWHM = 6.74 ± 0.06 kpc. This 2.5° wide stripe contains 0.9% as many F turnoff stars as the current Sagittarius dwarf galaxy. Over small spatial extent, the debris is modeled as a cylinder with a density that falls off as a Gaussian with distance from the axis, while the smooth component of the spheroid is modeled with a Hernquist profile. We assume that the absolute magnitude of F turnoff stars is distributed as a Gaussian, which is an improvement over previous methods which fixed the absolute magnitude at . The effectiveness and correctness of the algorithm is demonstrated on a simulated set of F turnoff stars created to mimic SDSS stripe 82 data, which shows that we have a much greater accuracy than previous studies. Our algorithm can be applied to divide the stellar data into two catalogs: one which fits the stream density profile and one with the characteristics of the spheroid. This allows us to effectively separate tidal debris from the spheroid population, both facilitating the study of the tidal stream dynamics and providing a test of whether a smooth spheroidal population exists.
Utility of neurophysiological monitoring using dorsal column mapping in intramedullary spinal cord surgery.

OBJECT Intramedullary spinal cord tumors can displace the surrounding neural tissue, causing enlargement and distortion of the normal cord anatomy. Resection requires a midline myelotomy to avoid injury to the posterior columns. Locating the midline for myelotomy is often difficult because of the distorted anatomy. Standard anatomical landmarks may be misleading in patients with intramedullary spinal cord tumors due to cord rotation, edema, neovascularization, or local scar formation. Misplacement of the myelotomy places the posterior columns at risk of significant postoperative disability. The authors describe a technique for mapping the dorsal column to accurately locate the midline. METHODS A group of 10 patients with cervical and thoracic intramedullary spinal cord lesions underwent dorsal column mapping in which a strip electrode was used to define the midline. After the laminectomy and durotomy, a custom-designed multielectrode grid was placed on the exposed dorsal surface of the spinal cord. The electrode is made up of 8 parallel Teflon-coated stainless-steel wires (76-microm diameter, spaced 1 mm apart) embedded in silastic with each of the wires stripped of its insulating coating along a length of 2 mm. This strip electrode maps the amplitude gradient of conducted spinal somatosensory evoked potentials elicited by bilateral tibial nerve stimulation. Using these recordings, the dorsal columns are topographically mapped as lying between two adjacent numbers. RESULTS The authors conducted a retrospective analysis of the preoperative, immediate, and short-term postoperative neurological status, focusing especially on posterior column function. There were 8 women and 2 men whose mean age was 52 years. There were 4 ependymomas, 1 subependymoma, 1 gangliocytoma, 1 anaplastic astrocytoma, 1 cavernous malformation, and 2 symptomatic syringes requiring shunting. In all patients the authors attempted to identify the midline by using anatomical landmarks, and then proceeded with dorsal column mapping to identify the midline electrophysiologically. In the 2 patients with syringomyelia and in 5 of the patients with tumors, the authors were unable to identify the midline anatomically with any certainty. In 2 patients with intramedullary tumors, they were able to identify the midline anatomically with certainty. Dorsal column mapping allowed identification of the midline and to confirm the authors' anatomical localization. In 2 patients with intramedullary tumors, posterior column function was preserved only on 1 side. All other patients had intact posterior column function preoperatively. CONCLUSIONS Dorsal column mapping is a useful technique for guiding the surgeon in locating the midline for myelotomy in intramedullary spinal cord surgery. In conjunction with somatosensory evoked potential, motor evoked potential, and D-wave recordings, we have been able to reduce the surgical morbidity related to dorsal column dysfunction in this small group of patients.
Mathematical modelling of infection and disease due to Neisseria meningitidis and Neisseria lactamica.

BACKGROUND Invasive meningococcal disease, due to Neisseria meningitidis, is an important cause of morbidity and mortality in young children and adolescents. Nasopharyngeal carriage of meningococci (MC), is most prevalent in young adults whereas carriage of Neisseria lactamica (LC), a related non-pathogenic organism, is most prevalent in young children. The objective of this study was to use modelling techniques to test hypotheses on the processes that govern the incidence of meningococcal disease (MD). METHODS Deterministic compartmental models were fitted to age structured data sets of MC, LC and MD. RESULTS The model most consistent with the available data sets is one where LC inhibits MC, an inhibition that lasts for a mean of 4.7 years. The hypothesis that LC also acts as a natural immunogen against MD was consistent with this model. The second peak of MD observed among adolescents could be due to the peak in the acquisition of MC in this age group. CONCLUSIONS The role of LC as a natural immunogen against asymptomatic and symptomatic meningococcal infection was consistent with available field data. If the introduction of novel meningococcal vaccines into a population changes the prevalence of MC or LC, this could have a substantial impact on the effectiveness of immunization programmes. This paper demonstrates the potential utility of modelling to estimate these effects.