Miracle-dz/UniFAR-FTUs huggingface dataset:
Widespread solid particle formation by mountain waves in the Arctic stratosphere

Observations of polar stratospheric clouds (PSCs) by lidar show that the clouds often contain solid particles, which are most likely composed of nitric acid hydrates. However, laboratory experiments indicate that such hydrate particles are not easily formed under Arctic synoptic scale conditions, suggesting that solid PSC particles should be rather rare. Here we show results from a model study indicating that mountain-induced mesoscale temperature perturbations may be an important source of nitric acid hydrate particles in the Arctic. Multiple Arctic vortex trajectories were combined with a global mountain wave forecast model to calculate the potential for solid particle formation during December and January 1994/1995. The mountain wave model was used to calculate adiabatic cooling over several thousand ridge elements. Nitric acid hydrate particles were assumed to form in the mountain waves according to several microphysical mechanisms, and were then advected using polar vortex-filling synoptic trajectories to generate maps of solid particle occurrence. The calculations show that mountain waves may be a significant source of PSCs containing solid particles that are observed on the synoptic scale. In particular, the east coast of Greenland, the Norwegian mountains, and the Urals are found to be solid particle sources, with the PSCs often predicted to survive several thousand kilometers downstream.
Predominance of Single Prophage Carrying a CRISPR/cas System in “Candidatus Liberibacter asiaticus” Strains in Southern China

“Candidatus Liberibacter asiaticus” (CLas) is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature. This study began with the genome sequence analysis of CLas Strain A4 from Guangdong in the prophage region. Within the two currently known prophage types, Type 1 (SC1-like) and Type 2 (SC2-like), A4 genome contained only a Type 2 prophage, CGdP2, namely. An analysis on CLas strains collected in Guangdong showed that Type 2 prophage dominated the bacterial population (82.6%, 71/86). An extended survey covering five provinces in southern China also revealed the predominance of single prophage (Type 1 or Type 2) in the CLas population (90.4%, 169/187). CLas strains with two and no prophage types accounted for 7.2% and 2.8%, respectively. In silico analyses on CGdP2 identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) system, consisting of four 22 bp repeats, three 23 bp spacers and 9 predicted cas. Similar CRISPR/cas systems were detected in all 10 published CLas prophages as well as 13 CLas field strains in southern China. Both Type 1 and Type 2 prophages shared almost identical sequences in spacer 1 and 3 but not spacer 2. Considering that the function of a CRISPR/cas system was to destroy invading DNA, it was hypothesized that a pre-established CLas prophage could use its CRISPR/cas system guided by spacer 1 and/or 3 to defeat the invasion of the other phage/prophage. This hypothesis explained the predominance of single prophage type in the CLas population in southern China. This is the first report of CRISPR/cas system in the “Ca. Liberibacter” genera.
Influence of formalin fixation on the biomechanical properties of human diaphyseal bone

Abstract Owing to the lack of fresh human bones, formalin-fixed specimens are frequently used in biomechanical testing. However, formalin fixation is assumed to affect the biomechanical properties of bone. The aim of this study was to compare axial and torsional stiffness and bone mineral density in fresh and embalmed human bones. The subtrochanteric regions of 12 pairs of fresh human femora were randomised into two groups for paired comparison. After bone mineral density measurement, one group was preserved in 4% formalin. After 6 weeks, bone mineral density was remeasured and each specimen underwent axial and torsional loading. The formalin group showed significant higher stiffness values for torsional and axial loads than the fresh group. Axial stiffness increased by 14.1%, whereas torsional stiffness increased by 14.3%. These differences were not reflected in bone mineral density values. Formalin fixation significantly influences the stiffness of human cadaveric bones. Fresh bones represent the in vivo conditions better than formalin fixed bones.
Construction and characterization in vivo of Bordetella pertussis aroA mutants

A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector system based on the suicide vector pRTP1 was used to deliver the mutations from E. coli back into B. pertussis CN2992FS and BP1. The aroA mutation was introduced by allelic exchange into the chromosome of B. pertussis, resulting in otherwise isogenic parental and aroA mutant pairs. The B. pertussis aroA mutants grew well on laboratory medium supplemented with aromatic compounds but failed to grow on unsupplemented medium. The B. pertussis aroA mutants expressed the normal B. pertussis extracellular, virulence-associated proteins; inactivated, whole-cell vaccines prepared from the mutants protected mice as efficiently as vaccines made from the parent strains against intracerebral challenge with the virulent B. pertussis 18323. Live B. pertussis aroA bacteria inefficiently colonized the lungs of NIH/S mice after they were challenged with aerosol, unlike the wild-type B. pertussis organism. Mice exposed to three separate aerosols of live B. pertussis aroA bacteria were protected against lung colonization after being exposed to an aerosol containing the virulent parental B. pertussis strain. High-level antibodies against B. pertussis rapidly appeared in the sera of mice immunized by aerosol with the B. pertussis aroA strains and challenged with the virulent parent.
Loose coupling in the bacterial flagellar motor

Significance Many species of bacteria swim using rotating helical propellers called flagella. Flagellar rotary motors obtain energy from the transmembrane gradient of ions (either H+ or Na+). Physiological properties of the motor have been studied for more than 40 y and have led to the longstanding view that the motor is tightly coupled, meaning that each revolution is associated with the movement of a fixed number of ions across the membrane. Simulations described here indicate that this may not be the case: A loosely coupled mechanism can explain all of the observations that have been taken to indicate tight coupling, as well as some measurements that are more difficult to account for within the tight-coupling framework. Physiological properties of the flagellar rotary motor have been taken to indicate a tightly coupled mechanism in which each revolution is driven by a fixed number of energizing ions. Measurements that would directly test the tight-coupling hypothesis have not been made. Energizing ions flow through membrane-bound complexes formed from the proteins MotA and MotB, which are anchored to the cell wall and constitute the stator. Genetic and biochemical evidence points to a “power stroke” mechanism in which the ions interact with an aspartate residue of MotB to drive conformational changes in MotA that are transmitted to the rotor protein FliG. Each stator complex contains two separate ion-binding sites, raising the question of whether the power stroke is driven by one, two, or either number of ions. Here, we describe simulations of a model in which the conformational change can be driven by either one or two ions. This loosely coupled model can account for the observed physiological properties of the motor, including those that have been taken to indicate tight coupling; it also accords with recent measurements of motor torque at high load that are harder to explain in tight-coupling models. Under loads relevant to a swimming cell, the loosely coupled motor would perform about as well as a two-proton motor and significantly better than a one-proton motor. The loosely coupled motor is predicted to be especially advantageous under conditions of diminished energy supply, or of reduced temperature, turning faster than an obligatorily two-proton motor while using fewer ions.
Comparison of Chewing Ability, Oral Health Related Quality of Life and Nutritional Status Before and After Insertion of Complete Denture amongst Edentulous Patients in a Dental College of Pune

Background The relationship between tooth loss and nutritional intake is important. As people age, their diminished physical capacity and decreased income adversely affect their ability to maintain their teeth. The aim of the study was to assess and compare the chewing ability, oral health related quality of life and nutritional status before and after fabrication and insertion of complete denture amongst edentulous participants in a dental college. Material and Methods Non Randomized Intervention study. The study population consisted of 42 participants (16 females and 26 males), aged 50 years and above. Prior to commencement of the study, informed consent was obtained and validation and reliability test of the questionnaire were done. The data for chewing ability, GOHAI and nutritional status assessment was recorded at baseline, 3rd, 6th and 12th month after denture fabrication and insertion. The statistical comparisons were performed by repeated measure ANOVA and Chi-square test. P value<0.05 was considered as statistically significant. Results Chewing ability, GOHAI, BMI (Body Mass Index) and data from Food-intake questionnaire showed statistically significant improvement from baseline to 6th month but no statistically significant improvement was observed from 6th month to 12th month. Nutritive value of food (protein, energy and fat) showed no significant difference over a period of 12 months (p<0.05). Conclusion Thus, it was concluded that the intervention (denture insertion) was effective in increasing the chewing ability, body weight, food-intake, and oral health related quality of life.
Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

Backgrounds/Aims: Colorectal cancer (CRC) cells show some alterations of lipid metabolism. Elongation of fatty acids (FA) has not been studied in CRC tissues thus far. The aim of this study was to verify if CRC specimens and normal colon mucosa differ in terms of their levels of very long-chain FAs, a product of FA elongation. Moreover, the expression of elongase genes has been studied in normal tissue and CRC. Finally, we searched for some specific products of FA elongation in serum of CRC patients. Methods: The specimens of normal colon mucosa and CRC were obtained from nineteen CRC patients differ in terms of FA elongation. We also searched for some specific products of FA elongation in serum of CRC patients and from healthy volunteers. Tissue and serum FA profiles were determined by means of gas chromatography-mass spectrometry (GC/MS), and the tissue expression of elongases (ELOVLs) was analyzed with real-time PCR. Results: Compared to normal colon tissue, CRC specimens showed significantly higher levels of 22-, 24- and 26-carbon FAs, stronger expressions of ELOVL1 and ELOVL6 (4- and 9-fold elevated respectively), and higher values of 18: 0/16: 0 elongation index. We also demonstrated presence of cerotic acid (26: 0) in serum of all CRC patients but in none of the healthy controls. Conclusions: CRC tissue seems to be characterized by enhanced FA elongation (hyper-elongation). Presence of cerotic acid in CRC patients sera and absence of this FA in healthy subjects points to this compound as a strong candidate for specific metabolic marker of colorectal malignancies.
Health related quality of life of long-term kidney transplantation recipients.

BACKGROUND Health related quality of life (HRQOL) is an important issue for long-term kidney transplantation (KT) patients. Nevertheless, few studies have focused on long-term HRQOL in KT recipients with a functional graft. Thus, the aim of this study is to describe the long-term (10-year) HRQOL of KT recipients. METHODS This is a cross-sectional and correlational design. The Medical Outcome Survey (MOS SF-36) questionnaire was used to collect data on HRQOL. The data were collected from November 2009 to September 2010 at a medical center in Northern Taiwan. RESULTS A total of 88 patients were interviewed. The mean years after transplantation was 14.48 (SD = 3.9). The mean score of each of the HRQOL subscales ranged from 59.4 to 82.5. The mean scores on the bodily pain (BP) subscale were the highest and, on the general health (GH) subscale, the lowest. Compared to the general population, with the exception of the BP subscale, long-term KT patients had a lower mean score (poorer HRQOL) on all subscales. Age, gender, serum creatinine level, and employment status were significantly related to HRQOL. CONCLUSION HRQOL of long-term KT patients was, overall, poorer than that of the general population. When comparing the HRQOL of KT patients with that of the general population, one should take into account age and gender. Finally, the physical, psychological, and social adjustment domains of HRQOL of KT patients warrant further attention.
Low-dose azathioprine is effective in maintaining remission in steroid-dependent ulcerative colitis: results from a territory-wide Chinese population-based IBD registry

Background: Whether low-dose azathioprine (AZA) is effective in maintaining remission in patients with steroid-dependent ulcerative colitis (UC) remains unclear. We assessed the efficacy and safety of low-dose AZA in a Chinese population with UC. Methods: We identified steroid-dependent UC patients in clinical remission on AZA maintenance therapy from a territory-wide IBD Registry. Standard- and low-dose AZA were defined as at least 2 mg/kg/day and less than 2 mg/kg/day, respectively. Relapse rates were analyzed by Kaplan–Meier analysis and compared using log-rank test. Results: Among 1226 UC patients, 128 (53% male, median duration on AZA 44 months) were included. Median maintenance AZA dose was 1.3 mg/kg/day. 97.7% of the patients were on concomitant oral 5-aminosalicylic acid. Cumulative relapse-free rates in patients on standard-dose and low-dose AZA were 71.2%, 52.8% and 45.2%, and 71.8%, 55.3% and 46.2% at 12, 24 and 36 months, respectively (p = 0.871). Relapse rate within 12 months was higher in patients who withdrew compared with those who maintained on AZA (52.6% versus 29.4%; p = 0.045). Mean corpuscular volume increased after AZA therapy in both of the low-dose [median (interquartile range, IQR): 88.2 (81.4–92.2) versus 95.1 (90.1–100.9) fl, p < 0.001] and standard-dose subgroups [median (IQR) 86.8 (76.9–89.9) versus 94.7 (85.9–99.7) fl, p < 0.001]. Leukopenia occurred in 21.1% of the patients. Patients on standard dose had a higher risk for leukopenia than those on low-dose AZA [odds ratio (OR) 3.9, 95% CI 1.9–8.2, p < 0.001]. Conclusions: In the Chinese population, low-dose AZA is effective for maintaining remission in steroid-dependent UC patients. Standard-dose AZA was associated with more than threefold increased risk of leukopenia.
Sometimes fish, sometimes fowl? Liminality, identity work and identity malleability in graduate teaching assistants

Abstract Graduate teaching assistants (GTAs) have been described as being ‘neither fish nor fowl’, occupying a role between student and teacher. Their multiple identities are commonly framed within the literature as a key challenge. This study explored the perspectives of GTAs when discussing their teaching work, through activity-oriented focus groups with nine GTAs from a UK university. Thematic analysis revealed that whilst GTAs showed a lack of clarity over their identity, they are actively involved in the process of ‘identity work’ through negotiating an emerging professional identity. Furthermore, liminality of status, being neither fully a student nor teacher, allows GTAs to operate with identity malleability, adjusting their most salient identity to meet the demands of the situation. It is argued that rather than occupying an ‘ambiguous niche’, GTAs occupy a ‘unique niche’ and the identity malleability they possess affords the optimum conditions in which to engage in identity work.
Zygosity Diagnosis in Young Twins by Questionnaire for Twins' Mothers and Twins' Self-reports

Abstract The present study deals with the determination of zygosity in twins of childhood age by simple questionnaire. The subjects were 224 twin pairs and their mothers, consisting of 159 monozygotic and 65 same-sex dizygotic pairs, identified by genetic markers including DNA samples. Mothers of twins responded to 19 questionnaire items dealing with twin similarity in 16 items about physical features and 3 items about the degree of similarity and frequency of being mistaken (confusion of identity) when twins were about 1 year of age. The twins themselves responded to three questionnaire items dealing with only confusion of identity items. The results of stepwise logistic regression analysis were as follows: the total accuracy of the mothers' questionnaire was 91.5% when using only the items dealing with confusion of identity. This accuracy was slightly lower than that obtained by twins' self-reports dealing with nearly the same question items of confusion of identity, answered by both twins separately with 93.3% accuracy. The total accuracy of mothers' questionnaire responses rose to 95.1% when we used all 19 items. In addition to ‘the frequency of being mistaken‘, two physical features, namely ‘shape of fingers’ and ‘shape of eyebrow’, were very informative. In conclusion, twin zygosity can be estimated by the use of the mothers' simple questionnaire with sufficient accuracy even in very young twins about 1 year of age.
Failure of cervical ripening with prostaglandin-E2 can it be predicted?

Objective. To identify factors predicting failure of cervical ripening with prostaglandin E2 (PGE2). Study design. A retrospective, cohort study of all women admitted for preinduction cervical ripening and failed to respond to PGE2 in comparison with randomly selected control group of women who underwent successful preinduction cervical ripening with PGE2 in a 3:1 ratio. Results. Overall 488 women were included, of them, 122 in the study group and 366 in the control group. A comparison between the two groups has revealed that maternal age above 30 years (OR = 2.7, 95%-CI 1.3–5.6), nulliparity (OR = 4.1, 95%-CI 1.7–10.0), prepregnancy BMI above 25 kg/m2 (OR = 3.5, 95%-CI 1.7–7.1), cervical dilatation of 1 cm (OR = 9.1, 95%-CI 3.5–13.4), cervical effacement of 50% or less admission (OR = 5.0, 95%-CI 2.2–8.8), and gestational age of 37 weeks or less (OR = 2.9, 95%-CI 1.3–6.6), are independent and significant predictors of cervical ripening failure with PGE2, and account for almost 50% of the variation in ripening outcome (R2 = 0.47). No association was found between the risk of cervical ripening failure and the indication for ripening, rupture of membranes, weight gain during pregnancy, weight before labor, diabetes or preeclampsia during pregnancy, and the newborn weight. Conclusions. Characterization of women who have a high probability for cervical ripening failure with PGE2 will help to improve consultation and may help choosing the optimal method for labor induction in these cases.
Early Exposure to Violence, Relationship Violence, and Relationship Satisfaction in Adolescents and Emerging Adults: The Role of Romantic Attachment

Objective: Violence in romantic relationships is highly prevalent in adolescence and early adulthood and is related to a wide array of negative outcomes. Although the scientific literature increasingly highlights potential risk factors for the perpetration of violence toward a romantic partner, integrative models of these predictors remain scarce. Using an attachment framework, the current study examines the associations between early exposure to violence, perpetration of relationship violence, and relationship satisfaction. We hypothesized that exposure to family violence fosters the development of attachment anxiety and avoidance, which in turn are related to relationship violence and low relationship satisfaction. Method: At Time 1, a sample of 1,252 (72.3% women) adolescents and emerging adults were recruited from high schools and colleges. Participants completed measures of exposure to family violence, attachment, perpetrated relationship violence and relationship adjustment. Three years later (Time 2), 234 of these participants agreed to participate in a follow-up assessment. Structural equation modeling was used to test cross-sectional and longitudinal models. Results: The findings suggest that exposure to family violence predicts relationship violence both directly and indirectly through attachment anxiety, whereas attachment avoidance and relationship violence are predictors of relationship satisfaction. Longitudinal analyses also show that changes in romantic attachment are associated with changes in relationship violence and satisfaction. Conclusions: Romantic attachment is a significant target for the prevention and treatment of violence in intimate relationships involving adolescents or emerging adults.
Simultaneous Hypoxia and Low Extracellular pH Suppress Overall Metabolic Rate and Protein Synthesis In Vitro

Background The tumor microenvironment is characterized by regions of hypoxia and acidosis which are linked to poor prognosis. This occurs due to an aberrant vasculature as well as high rates of glycolysis and lactate production in tumor cells even in the presence of oxygen (the Warburg effect), which weakens the spatial linkage between hypoxia and acidosis. Methods Five different human squamous cell carcinoma cell lines (SiHa, FaDuDD, UTSCC5, UTSCC14 and UTSCC15) were treated with hypoxia, acidosis (pH 6.3), or a combination, and gene expression analyzed using microarray. SiHa and FaDuDD were chosen for further characterization of cell energetics and protein synthesis. Total cellular ATP turnover and relative glycolytic dependency was determined by simultaneous measurements of oxygen consumption and lactate synthesis rates and total protein synthesis was determined by autoradiographic quantification of the incorporation of 35S-labelled methionine and cysteine into protein. Results Microarray analysis allowed differentiation between genes induced at low oxygen only at normal extracellular pH (pHe), genes induced at low oxygen at both normal and low pHe, and genes induced at low pHe independent of oxygen concentration. Several genes were found to be upregulated by acidosis independent of oxygenation. Acidosis resulted in a more wide-scale change in gene expression profiles than hypoxia including upregulation of genes involved in the translation process, for example Eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), and Ribosomal protein L37 (RPL37). Acidosis suppressed overall ATP turnover and protein synthesis by 50%. Protein synthesis, but not total ATP production, was also suppressed under hypoxic conditions. A dramatic decrease in ATP turnover (SiHa) and protein synthesis (both cell lines) was observed when hypoxia and low pHe were combined. Conclusions We demonstrate here that the influence of hypoxia and acidosis causes different responses, both in gene expression and in de novo protein synthesis, depending on whether the two factors induced alone or overlapping, and as such it is important for in vivo studies to take this into account.