Miracle-dz/UniFAR-FTUs huggingface dataset:
Rugged Single Domain Antibody Detection Elements for Bacillus anthracis Spores and Vegetative Cells

Significant efforts to develop both laboratory and field-based detection assays for an array of potential biological threats started well before the anthrax attacks of 2001 and have continued with renewed urgency following. While numerous assays and methods have been explored that are suitable for laboratory utilization, detection in the field is often complicated by requirements for functionality in austere environments, where limited cold-chain facilities exist. In an effort to overcome these assay limitations for Bacillus anthracis, one of the most recognizable threats, a series of single domain antibodies (sdAbs) were isolated from a phage display library prepared from immunized llamas. Characterization of target specificity, affinity, and thermal stability was conducted for six sdAb families isolated from rounds of selection against the bacterial spore. The protein target for all six sdAb families was determined to be the S-layer protein EA1, which is present in both vegetative cells and bacterial spores. All of the sdAbs examined exhibited a high degree of specificity for the target bacterium and its spore, with affinities in the nanomolar range, and the ability to refold into functional antigen-binding molecules following several rounds of thermal denaturation and refolding. This research demonstrates the capabilities of these sdAbs and their potential for integration into current and developing assays and biosensors.
Differential sympathetic-parasympathetic interactions in sinus node and AV junction.

This study examined the effects and interactions evoked by various combinations of parasympathetic (P) and sympathetic (S) stimulations on sinus node automaticity and atrioventricular (AV) conduction (both anterograde and retrograde) in 11 two-mo old anesthetized puppies. The respective effects of these autonomic maneuvers were assessed by covariant analysis using a linear regression model of the form Y = alpha 0 + alpha 1 X base line + alpha 2 X sequence + alpha 3 X S + alpha 4 X S2 + alpha 5 X P + alpha 6 X P2 + alpha 7 X (P X S). The effects of parasympathetic and sympathetic stimulation, of the quadratic term S2, and of the interaction term (P X S) were highly significant (P less than 0.0001) on both sinus node automaticity and AV conduction (anterograde and retrograde). In contrast, the effect of the quadratic term P2 was significant on sinus node automaticity only. Sequence of stimulation whether (SP) or (PS) had no significant effect on either chronotropic or dromotropic properties. Furthermore, whereas augmented antagonism with parasympathetic predominance over sympathetic activity was readily demonstrable in the control of sinus rate, the reverse, i.e., augmented antagonism with sympathetic preponderance over parasympathetic activity, was found to apply to the control of AV conduction (both anterograde and retrograde). Hence, the nature and extent of parasympathetic-sympathetic interactions vary considerably, depending on which cardiac structure and/or function is under scrutiny. It appears that AV conduction is especially sensitive to modulation of sympathoadrenergic activity, whereas sinus node automaticity is particularly responsive to cholinomimetic influences.
Loss of pigment epithelium derived factor expression in glioma progression

Background: Pigment epithelium derived factor (PEDF) was first isolated from medium conditioned by human fetal retinal pigment epithelial cells. PEDF was detected in a broad range of human fetal and adult tissues including almost all brain areas. It can also inhibit the proliferation of cultured rat astrocytes. Recent studies have implicated PEDF in activities that are inhibitory to angiogenesis. Aims: To investigate the expression of PEDF in gliomas to assess its “gliastatic” effects and its role in anti-angiogenesis. Methods: PEDF mRNA values were measured by quantitative real time reverse transcription polymerase chain reaction (RT-PCR) analysis of normal brain tissue and tumour specimens from both low and high grade gliomas. In addition, immunohistochemical staining for PEDF and vascular endothelial growth factor (VEGF) was performed on 32 paraffin wax embedded glioma samples, 10 of them grade IV, 10 grade III, seven grade II, and five grade I. Results: RT-PCR showed that PEDF mRNA values were 5.0 (p < 0.001) and 15.4 (p < 0.001) times higher in normal human brain specimens (n = 5) than in tumour tissue specimens of low grade glioma (grades I and II; n = 15) and high grade glioma (grades III and IV; n = 10), respectively. VEGF was strongly positive in 90% of grade IV, 70% of grade III, 43% of grade II, and 20% of grade I cases. In contrast, PEDF was positive in none of grade IV, 20% of grade III, 43% of grade II, and 60% of grade I tumours. There was an inverse correlation between VEGF and PEDF expression, and a lack of PEDF in advanced grade gliomas. Conclusions: It is possible that the absence of PEDF expression is a potent factor for the enhancement of angiogenesis in glioma.
Cardiometabolic aspects of polycystic ovarian syndrome

It is estimated that 6%–7% of women of reproductive age have polycystic ovarian syndrome (PCOS). Women with this condition exhibit an adverse cardiovascular risk profile, characteristic of the cardiometabolic syndrome and given the high prevalence of PCOS in the female population, this condition may contribute towards the acceleration of cardiovascular disease among young women. This article summarizes the recent development and findings in the cardiometabolic abnormalities in patients with PCOS. Patients with PCOS have the clinical features of oligomenorrhoea, hirsutism and infertility; however, they also exhibit hyperinsulinemia, obesity, hypertension, dyslipidemia, and an increased pro-thrombotic state. They have an increased risk of type 2 diabetes and impaired glucose tolerance, and sleep apnea is also found more commonly in this population. However, despite the presence of cardiovascular risk factors and increased surrogate markers of cardiovascular disease it is unclear if they have accelerated atherosclerosis. End point studies are currently lacking and the available evidence are conflicting. Adipose tissue has emerged as an important endocrine organ over the last decade and gained recognition in having an important role in the cardiometabolic syndrome. Adiponectin that is secreted exclusively by adipocytes has recently been recognized as an important marker of cardiometabolic syndrome, obesity, type 2 diabetes, and coronary artery disease. Other adipocytokines like leptin and resistin have also recently been recognized. This article will address the current evidence for the adverse cardiovascular risk in PCOS and the other factors that may be implicated. Finally the therapeutic options for treatment will be discussed.
THE MASS DISTRIBUTION AND ASSEMBLY OF THE MILKY WAY FROM THE PROPERTIES OF THE MAGELLANIC CLOUDS

We present a new measurement of the mass of the Milky Way (MW) based on observed properties of its largest satellite galaxies, the Magellanic Clouds (MCs), and an assumed prior of a ΛCDM universe. The large, high-resolution Bolshoi cosmological simulation of this universe provides a means to statistically sample the dynamical properties of bright satellite galaxies in a large population of dark matter halos. The observed properties of the MCs, including their circular velocity, distance from the center of the MW, and velocity within the MW halo, are used to evaluate the likelihood that a given halo would have each or all of these properties; the posterior probability distribution function (PDF) for any property of the MW system can thus be constructed. This method provides a constraint on the MW virial mass, 1.2+0.7− 0.4 (stat.)+0.3− 0.3 (sys.) × 1012 M☉ (68% confidence), which is consistent with recent determinations that involve very different assumptions. In addition, we calculate the posterior PDF for the density profile of the MW and its satellite accretion history. Although typical satellites of 1012 M☉ halos are accreted over a wide range of epochs over the last 10 Gyr, we find a ∼72% probability that the MCs were accreted within the last Gyr, and a 50% probability that they were accreted together.
Relationships between spatio‐temporal environmental and genetic variation reveal an important influence of exogenous selection in a pupfish hybrid zone

The importance of exogenous selection in a natural hybrid zone between the pupfishes Cyprinodon atrorus and Cyprinodon bifasciatus was tested via spatio‐temporal analyses of environmental and genetic change over winter, spring and summer for three consecutive years. A critical influence of exogenous selection on hybrid zone regulation was demonstrated by a significant relationship between environmental (salinity and temperature) and genetic (three diagnostic nuDNA loci) variation over space and time (seasons) in the Rio Churince system, Cuatro Ciénegas, Mexico. At sites environmentally more similar to parental habitats, the genetic composition of hybrids was stable and similar to the resident parental species, whereas complex admixtures of parental and hybrid genotypic classes characterized intermediate environments, as did the greatest change in allelic and genotypic frequencies across seasons. Within hybrids across the entire Rio Churince system, seasonal changes in allelic and genotypic frequencies were consistent with results from previous reciprocal transplant experiments, which showed C. bifasciatus to suffer high mortality (75%) when exposed to the habitat of C. atrorus in winter (extreme temperature lows and variability) and summer (abrupt salinity change and extreme temperature highs and variability). Although unconfirmed, the distributional limits of C. atrorus and C. atrorus‐like hybrids appear to be governed by similar constraints (predation or competition). The argument favouring evolutionary significance of hybridization in animals is bolstered by the results of this study, which links the importance of exogenous selection in a contemporary hybrid zone between C. atrorus and C. bifasciatus to previous demonstration of the long‐term evolutionary significance of environmental variation and introgression on the phenotypic diversification Cuatro Ciénegas Cyprinodon.
Calcification of the interclinoid and petroclinoid ligaments of sella turcica: a radiographic study of the prevalence.

OBJECTIVES The purpose of this study was to evaluate the prevalence of calcifications of the sella turcica, in particular, calcification of the interclinoid and petroclinoid ligaments (PCL). DESIGN Radiographic analysis of preoperative cephalometric film images. SETTING AND SAMPLE POPULATION Lateral cephlometric radiographs of 255 subjects presenting for orthodontic evaluation were reviewed. The number of subjects selected for this study was determined by power analysis. EXPERIMENTAL VARIABLE Two calibrated raters reviewed cephalometric projections and scored the films using a standardized rating scale. OUTCOME MEASURE The rating scale classified interclinoid ligaments (ICL) into one of four categories depending on the degree of calcification and PCLs as either, no calcification, partial or complete calcification. RESULTS Of all subjects, calcification of the ICL ranged from 39% rated as more than half calcified to 8% completely calcified. Petroclinoid analysis revealed 67% with no calcification, 23% with partial calcification and 9% completely calcified. Spearman's correlations were computed between age and the degree of calcification and between the degree of calcification for these two ligaments with a significant association between age and degree of calcification in the PCL, r = 0.185 (p = 0.003) and a significant association between the degree of calcification in the petroclinoid and ICLs, r = 0.186 (p = 0.003). In addition, chi-squared tests demonstrated statistically significant associations between the presence of calcification in the PCL to the distribution of age (p = 0.041) and between the presence of calcification in the ICL to the distribution of age (p = 0.045). CONCLUSION As calcification of these ligaments has suggestive associations with disease entities, their recognition as a variant of normal anatomy should be evaluated when assessing cephalometric radiographs.
COMPLEX POWERS OF OPERATORS

We define the complex powers of a densely defined operator A whose resolvent exists in a suitable region of the complex plane. Generally, this region is strictly contained in an angle and there exists α ∈ (0, ∞ )s uch that the resolvent of A is bounded by O((1 + |λ|) α ) there. We prove that for some particular choices of a fractional number b, the negative of the fractional power (−A) b is the c.i. g. of an analytic semigroup of growth order r> 0.
Smoking and HIV-related Health Issues among Older HIV-positive Gay, Bisexual, and Other Men Who Have Sex with Men

The prevalence of cigarette smoking and the relations between smoking and HIV clinical markers, HIV medication adherence, and opportunistic infections (OIs) were examined in a sample of 199 HIV-positive, gay, bisexual, and other men who have sex with men (MSM) aged 50 and older. Overall, 35.7% were current smokers, 35.7% were former smokers, and 28.6% were never smokers. In the final multivariable polytomous logistic regression model controlling for age, income, and illicit drug use, current smokers were less likely to report an undetectable viral load as compared to never and former smokers. Relative to never smokers, former smokers were more likely to report respiratory OIs, and current smokers were more likely to report gastrointestinal OIs. This study demonstrates high prevalence of cigarette smoking among aging, HIV-positive MSM and provides additional evidence for a relationship between smoking and poorer HIV clinical markers. Targeted and tailored smoking cessation programs within the context of HIV care services are warranted.
Combination treatment with quercetin and resveratrol attenuates high fat diet-induced obesity and associated inflammation in rats via the AMPKα1/SIRT1 signaling pathway

Diet-induced obesity is associated with systemic inflammation, which is considered to originate predominantly from the adipose tissue. Quercetin and resveratrol are two dietary polyphenols that exhibit anti-inflammatory properties and anti-insulin resistance when administered in isolation or combination (CQR). It remains unknown whether CQR reduces high fat diet (HFD)-induced obesity and inflammation in rats. In the current study, 46 male Wistar rats were divided into two groups, one of which was fed a normal diet (ND, 5.4% fat, w/w) and one of which was fed a HFD (45% fat, w/w) for 3 weeks. Following removal of the 12 most obesity-resistant rats from the HFD group, the remaining rats were divided into two sub-groups: A HFD group and a HFD+CQR group (administered 120 mg/kg/day resveratrol and 240 mg/kg/day quercetin). The results revealed that the HFD+CQR group had significantly lower body weights at 11 weeks compared with the HFD group and had significantly reduced visceral adipose tissue weights and adipocyte sizes. Serum lipid profiles were also significantly ameliorated in the HFD+CQR group. CQR attenuated the expression of systemic proinflammatory adipokines, including leptin, tumor necrosis factor-α, monocyte chemoattractant protein-1 and interleukin-6. It also reduced the recruitment of mast cells to the epididyotic adipose tissue (EAT). Furthermore, CQR reversed the HFD-induced suppression of 5′-adenosine monophosphate-activated protein kinase α1 (AMPKα1) phosphorylation and sirtuin 1 (SIRT1) expression in EAT. In conclusion, CQR may suppress obesity and associated inflammation via the AMPKα1/SIRT1 signaling pathway in rats fed a HFD.
Dynamic initiation and dual-tree complex wavelet feature-based classification of motor imagery of swallow EEG signals

The use of motor imagery-based brain computer interface has recently been shown to have potential for rehabilitation. This paper proposes a novel scheme to detect motor imagery of swallow from electroencephalography (EEG) signals for dysphagia rehabilitation. The proposed scheme extracts features from the coefficients of dual-tree complex wavelet transform (DT-CWT). A novel sliding window-based peak localization scheme is proposed to dynamically locate the initiation of tongue movement from Electromyography (EMG) signal. Subsequently, effective time segments are extracted from EEG signal for classification based on the detected dynamic initiation location. Comparisons are made between our proposed scheme with that of the three existing approaches. The results based on six healthy subjects show that an increase in averaged accuracy of 9.95% is achieved. Further, an increase in averaged accuracy of 8.02% is resulted comparing our proposed scheme by using and not using the dynamic initiation to extract the time segments. Classification results using EMG data confirm that our results are not due to movements artifacts. Statistical tests with 95% confidence to estimate the accuracy on the respective action at chance level show that five out of six subjects performed above chance level for our proposed dynamic initiation and wavelet feature-based approach.
Hormone receptor expression and clinicopathologic features in male and female breast cancer.

BACKGROUND Male and female breast cancers were investigated for variation in the clinicopathologic characteristics and expression of steroid hormone receptors in the northeast of Iran. MATERIALS AND METHODS Tumor specimens of 17 males and 338 females with breast cancer were collected at the hospitals of Mashhad University of Medical Sciences. Immunohistochemical expression of hormone receptors and clinicopathologic features of breast cancer were compared between two groups. RESULTS The mean age in men was 15 years higher than women (p=0.000). Males and females were mainly in stage II and III respectively (p=0.007). Although more than 60% of male and female patients were grade II, the respective figures for grade I and III were 25% and 12.5% in men but 7.1% and 27.2% in women respectively (p=0.025). ER was significantly more positive in men against women; 82.3% versus 53.4% (p=0.016). The related measures for PR was 58.8% and 50.3%, respectively (p=0.424). Males also showed significantly more ER expression than postmenopausal females; 82.3% versus 48.9% (p=0.010). CONCLUSIONS Breast cancer in males and females contrasted in age at diagnosis, histological type, stage, grade and ER expression which emphasize they are separate diseases with different behaviors.
Heat-Sterllized Pd Fluid Blocks Leukocyte Adhesion and Increases Flow Velocity in Rat Peritoneal Venules

Data from cell culture experiments indicate that heat sterilization of peritoneal dialysis (PD) fluids produces cytotoxic glucose degradation products. The present vital microscopic study investigated the effects of different sterilization methods on the biocompatibility of PD fluids. Thus, heat-sterilized (commercially obtained and experimentally produced) and filter-sterilized PD fluids (pH = 5.30 5.40; 1.5% glucose) were compared with Tyrode buffer, with respect to the effects on microvascular blood flow velocity and leukocyte adhesion in the rat mesentery. Exteriorization of the mesentery produced a mild inflammation, known from the literature and characterized by the adhesive rolling of leukocytes along venular walls. Superfusion of the mesentery with filter-sterilized PD fluid had no significant effects on leukocyte rolling or flow velocity in venules 25 40 μm in diameter compared with buffer superfusion. Heat-sterilized PD fluid decreased the concentration of rolling leukocytes and increased flow velocity significantly, as compared with buffer and filter-sterilized PD fluid. The results indicate that heat sterilization of PD fluids produces substances that interact with microvascular tone and leukocyte-endothelial adhesion, which hypothetically could impair the acute, granulocyte-mediated defense against bacterial infections.
Extraction of decision rules via imprecise probabilities

Abstract Data analysis techniques can be applied to discover important relations among features. This is the main objective of the Information Root Node Variation (IRNV) technique, a new method to extract knowledge from data via decision trees. The decision trees used by the original method were built using classic split criteria. The performance of new split criteria based on imprecise probabilities and uncertainty measures, called credal split criteria, differs significantly from the performance obtained using the classic criteria. This paper extends the IRNV method using two credal split criteria: one based on a mathematical parametric model, and other one based on a non-parametric model. The performance of the method is analyzed using a case study of traffic accident data to identify patterns related to the severity of an accident. We found that a larger number of rules is generated, significantly supplementing the information obtained using the classic split criteria.
Sodium Absorption from the Exoplanetary Atmosphere of HD 189733b Detected in the Optical Transmission Spectrum

We present the first ground-based detection of sodium absorption in the transmission spectrum of an extrasolar planet. Absorption due to the atmosphere of the extrasolar planet HD 189733b is detected in both lines of the Na I doublet. High spectral resolution observations were taken of 11 transits with the High Resolution Spectrograph (HRS) on the 9.2 m Hobby-Eberly Telescope (HET). The Na I absorption in the transmission spectrum due to HD 189733b is (− 67.2 ± 20.7) × 10−5 deeper in the “narrow” spectral band that encompasses both lines relative to adjacent bands. The 1 σ error includes both random and systematic errors, and the detection is >3 σ. This amount of relative absorption in Na I for HD 189733b is ~3 times larger than that detected for HD 209458b by Charbonneau et al. (2002) and indicates that these two hot Jupiters may have significantly different atmospheric properties.
Fruit Coloration and Anthocyanin Biosynthesis after Bag Removal in Non-Red and Red Apples (Malus × domestica Borkh.)

In the present study, evolution of apple color (L* and a/b), the accumulation of anthocyanins and the activity of the related enzymes, phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI), dihydroflavonol4-reductase (DFR) and UDP-Glucose: flavonoid-3-O-galactosyl transferase (UFGT), were investigated in bagged non-red apple cultivars (‘Granny Smith’ and ‘Golden Delicious’) and red apple cultivars (‘Starkrimon’ and ‘Pink Lady’). Young fruits were bagged 40–45 days after flowering (DAF), and fruits of ‘Golden Delicious’ and ‘Starkrimon’ were uncovered and exposed to light 120 DAF, while those of ‘Granny Smith’ and ‘Pink Lady’ were exposed for 160 DAF. Results showed that cyanidin 3-galactoside (cy3-gal) was the most abundant anthocyanin in both non-red and red cultivars. Level of anthocyanins was higher in ‘Granny Smith’ than in ‘Golden Delicious’, indicating that red color was easier to develop in green cultivar ‘Granny Smith’ than in yellow cultivar ‘Golden Delicious’ after bag removal. The cy3-gal accumulation of non-red cultivars tested was not significantly correlated with PAL, CHI and DFR activity, but was significantly correlated with UFGT activity. During the reddening of non-red apples, UFGT may be the more important factor in the anthocyanin biosynthesis.