Miracle-dz/UniFAR-FTUs huggingface dataset:
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)

The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.
Swallowing sounds in speech therapy practice: a critical analysis of the literature

ABSTRACT This study aimed to investigate international scientific papers published on the subject of cervical auscultation and its use in speech therapy. The study involved a qualitative review of the literature spanning the last 10 years. Articles were selected from the PubMed database using the following keywords: cervical auscultation, swallowing and swallowing disorders. Research was included that was conducted on adult humans (over 18 years of age) and was written in English. Each citation retrieved from the database was analyzed independently by each of the study researchers to ascertain its relevance for inclusion in the study. The methodology involved formulating the research question, locating and selecting studies and critically evaluating the articles according to the precepts of the Cochrane Handbook. As a result, 35 studies were identified; 13 articles were analyzed because they allowed access to the full text and were related directly to the subject. We found that the studies were performed with groups of healthy subjects and subjects with different types of base pathology. Some studies compared the patterns found in the different groups. Some of the research sought to study the pattern of swallowing sounds with different factors - evaluator experience, the specificity and sensitivity of the method and how to improve the technique of cervical auscultation through the use of instruments other than the stethoscope. The conclusion of this critical analysis is that cervical auscultation is an important tool to be used in conjunction with other assessment methods in the routine clinical evaluation of swallowing.
Thinking Through: Essays on Feminism, Marxism, and Anti-Racism

Brings together new and recent writings by writer and academic Himani Bannerji. Through anti-racist, Marxist feminism, Bannerji questions the notion of 'distinct/separate oppressions' which understands gender, race, and class as separate issues. Incisive and important, 'Thinking Through' offers a new strategy to theorising gender, race, class, and socialist revolution.
On the Order of Round Components in the AES

This paper analyses all 24 possible round constructions using different combinations of the four round components of the AES cipher: SubBytes, ShiftRows, AddRoundKey and MixColumns. We investigate how the different round orderings affect the security of AES against differential, linear, multiset, impossible differential and boomerang attacks. The cryptographic strenght of each cipher variant was measured by the size of each distinguisher, their probability or correlation value and the number of active S-boxes. Our analyses indicate that all these permutations of the AES components have similar cryptographic strength (concerning these five attacks), although there are implementation advantages for certain permutations.
COSMIC EVOLUTION OF BLACK HOLES AND SPHEROIDS. V. THE RELATION BETWEEN BLACK HOLE MASS AND HOST GALAXY LUMINOSITY FOR A SAMPLE OF 79 ACTIVE GALAXIES

We investigate the cosmic evolution of the black hole (BH) mass–bulge luminosity relation using a sample of 52 active galaxies at z ∼ 0.36 and z ∼ 0.57 in the BH mass range of 107.4–109.1 M☉. By consistently applying multicomponent spectral and structural decomposition to high-quality Keck spectra and high-resolution Hubble Space Telescope images, BH masses (MBH) are estimated using the Hβ broad emission line combined with the 5100 Å nuclear luminosity, and bulge luminosities (Lbul) are derived from surface photometry. Comparing the resulting MBH − Lbul relation to local active galaxies and taking into account selection effects, we find evolution of the form MBH/Lbul∝(1 + z)γ with γ = 1.8 ± 0.7, consistent with BH growth preceding that of the host galaxies. Including an additional sample of 27 active galaxies with 0.5 < z < 1.9 taken from the literature and measured in a consistent way, we obtain γ = 0.9 ± 0.7 for the MBH − Lbul relation and γ = 0.4 ± 0.5 for the MBH–total host galaxy luminosity (Lhost) relation. The results strengthen the findings from our previous studies and provide additional evidence for host galaxy bulge growth being dominated by disk-to-bulge transformation via minor mergers and/or disk instabilities.
Bilateral Loudness Balancing and Distorted Spatial Perception in Recipients of Bilateral Cochlear Implants

Objective: To determine whether bilateral loudness balancing during mapping of bilateral cochlear implants (CIs) produces fused, punctate, and centered auditory images that facilitate lateralization with stimulation on single-electrode pairs. Design: Adopting procedures similar to those that are practiced clinically, direct stimulation was used to obtain most-comfortable levels (C levels) in recipients of bilateral CIs. Three pairs of electrodes, located in the base, middle, and apex of the electrode array, were tested. These electrode pairs were loudness-balanced by playing right-left electrode pairs sequentially. In experiment 1, the authors measured the location, number, and compactness of auditory images in 11 participants in a subjective fusion experiment. In experiment 2, the authors measured the location and number of the auditory images while imposing a range of interaural level differences (ILDs) in 13 participants in a lateralization experiment. Six of these participants repeated the mapping process and lateralization experiment over three separate days to determine the variability in the procedure. Results: In approximately 80% of instances, bilateral loudness balancing was achieved from relatively small adjustments to the C levels (⩽3 clinical current units). More important, however, was the observation that in 4 of 11 participants, simultaneous bilateral stimulation regularly elicited percepts that were not fused into a single auditory object. Across all participants, approximately 23% of percepts were not perceived as fused; this contrasts with the 1 to 2% incidence of diplacusis observed with normal-hearing individuals. In addition to the unfused images, the perceived location was often offset from the physical ILD. On the whole, only 45% of percepts presented with an ILD of 0 clinical current units were perceived as fused and heard in the center of the head. Taken together, these results suggest that distortions to the spatial map remain common in bilateral CI recipients even after careful bilateral loudness balancing. Conclusions: The primary conclusion from these experiments is that, even after bilateral loudness balancing, bilateral CI recipients still regularly perceive stimuli that are unfused, offset from the assumed zero ILD, or both. Thus, while current clinical mapping procedures for bilateral CIs are sufficient to enable many of the benefits of bilateral hearing, they may not elicit percepts that are thought to be optimal for sound-source location. As a result, in the absence of new developments in signal processing for CIs, new mapping procedures may need to be developed for bilateral CI recipients to maximize the benefits of bilateral hearing.
Does Fast Adaptive Modulation Always Outperform Slow Adaptive Modulation?

Link adaptation techniques are important modern and future wireless communication systems to cope with quality of service fluctuations in fading channels. These techniques require the knowledge of the channel state obtained with a portion of resources devoted to channel estimation instead of data and updated every coherence time of the process to be tracked. In this paper, we analyze fast and slow adaptive modulation systems with diversity and non-ideal channel estimation under energy constraints. The framework enables to address the following questions: (i) What is the impact of non-ideal channel estimation on fast and slow adaptive modulation systems? (ii) How to define a proper figure of merit which considers both resources dedicated to data and those to channel estimation? (iii) Does fast adaptive always outperform slow adaptive techniques? Our analysis shows that, despite the lower complexity and feedback rate, slow adaptive modulation (SAM) can achieve higher spectral efficiency than fast adaptive modulation (FAM) in the presence of energy constraint, diversity, and non-ideal channel estimation. In addition, SAM satisfies bit error outage requirements also in FAM-denied region.
Increased viral burden and cytopathicity correlate temporally with CD4+ T-lymphocyte decline and clinical progression in human immunodeficiency virus type 1-infected individuals

The rate of clinical progression is variable among individuals infected with human immunodeficiency virus type 1 (HIV-1). Changes in viral burden which correlate with disease status have been demonstrated in cross-sectional studies; however, a detailed longitudinal study of the temporal relationship between viral burden, CD4+ T-cell numbers, and clinical status throughout the course of infection has not been reported. Multiple longitudinal blood samples were obtained from four HIV-1-infected individuals with clinically divergent profiles. Levels of HIV-1 were measured in sequential samples of peripheral blood mononuclear cells, using both end-point dilution cultures and quantitative polymerase chain reaction methods. Serial HIV-1 isolates from each case were also evaluated to determine their biological properties in vitro. For the three patients with clinical progression, a dramatic increase in the level of HIV-1 was observed concurrent with or prior to a marked drop in CD4+ T lymphocytes. This increase in viral burden was temporally associated with the emergence of a more cytopathic viral phenotype. In contrast, consistently low levels of HIV-1 were observed in the one patient who was clinically and immunologically stable for more than a decade. Moreover, viral isolates from this patient were less cytopathic in vitro compared with HIV-1 isolates from those patients with disease progression. The temporal association between increased viral burden and CD4+ T-cell decline suggests a direct role for HIV-1 in the cytopathology of CD4+ T cells in vivo. Our results indicate that the pathogenic mechanisms responsible for CD4+ T-cell depletion may be related to both quantitative and qualitative changes in HIV-1.
The future in action: neurophysiological and behavioral evidence of anticipatory motor simulation.

The motor system can no longer be considered as a mere passive executive system of motor commands generated elsewhere in the brain. On the contrary, it is deeply involved in perceptual and cognitive functions and acts as an “anticipation device”. The present thesis investigates the anticipatory motor mechanisms occurring in two particular instances: i) when processing sensory events occurring within the peripersonal space (PPS); and ii) when perceiving and predicting others’actions. The first study provides evidence that PPS representation in humans modulates neural activity within the motor system, while the second demonstrates that the motor mapping of sensory events occurring within the PPS critically relies on the activity of the premotor cortex. The third study provides direct evidence that the anticipatory motor simulation of others’ actions critically relies on the activity of the anterior node of the action observation network (AON), namely the inferior frontal cortex (IFC). The fourth study, sheds light on the pivotal role of the left IFC in predicting the future end state of observed right-hand actions. Finally, the fifth study examines how the ability to predict others’ actions could be influenced by a reduction of sensorimotor experience due to the traumatic or congenital loss of a limb. Overall, the present work provides new insights on: i) the anticipatory mechanisms of the basic reactivity of the motor system when processing sensory events occurring within the PPS, and the same anticipatory motor mechanisms when perceiving others’ implied actions; ii) the functional connectivity and plasticity of premotor-motor circuits both during the motor mapping of sensory events occurring within the PPS and when perceiving others’ actions; and iii) the anticipatory mechanisms related to others’ actions prediction.
Chandra Study of X-Ray Point Sources in the Early-Type Galaxy NGC 4552 (M89)

We present a Chandra ACIS study of the early-type galaxy NGC 4552. We detect 47 X-ray point sources, most of which are likely low-mass X-ray binaries (LMXBs), within four effective radii (Re). The brightest X-ray source coincides with the optical, UV, and radio center of the galaxy and shows variability on >1 hr timescales, indicating the possible existence of a low-luminosity active galactic nucleus (AGN). The 46 off-center sources and the unresolved point sources contribute about 29% and 20% to the total luminosity of the galaxy, respectively. We find that after correcting for the incompleteness at the low-luminosity end, the observed cumulative X-ray luminosity function (XLF) of the off-center sources is best fitted by a broken power-law model with a break at Lb = 4.4 × 1038 ergs s-1. We identified 210 globular cluster (GC) candidates in a HST WFPC2 optical image of the galaxy's central region. Of the 25 off-center LMXBs that fall within the WFPC2 field of view, 10 sources are coincident with a GC. Thus, the fraction of the GCs hosting bright LMXBs and the fraction of the LMXBs associated with GCs are 4.8% and 40%, respectively. In the V and I bands, the GCs hosting bright LMXBs are typically 1-2 mag brighter than the GCs with no detected LMXBs. There are about 1.9 ± 0.4 times as many LMXBs in the red, metal-rich GCs as there are in the blue, metal-poor ones. We find no obvious difference between the luminosity distributions of LMXBs in GCs and in the field, but the cumulative spectrum of the LMXBs in GCs tends to be softer than that of the LMXBs in field. We detected three X-ray sources that have isotropic luminosities larger than 1039 ergs s-1. Only one of these is located in the joint Chandra-HST field and is found to be associated with a GC. By studying its ACIS spectra we infer that the this may be a candidate black hole system with a mass of 15-135 M☉. One of the other sources with a luminosity brighter than 1039 ergs s-1 reveals temporal variations in brightness on timescales greater than 1 hr.
Multisensory Integration of Dynamic Faces and Voices in Rhesus Monkey Auditory Cortex

In the social world, multiple sensory channels are used concurrently to facilitate communication. Among human and nonhuman primates, faces and voices are the primary means of transmitting social signals (Adolphs, 2003; Ghazanfar and Santos, 2004). Primates recognize the correspondence between species-specific facial and vocal expressions (Massaro, 1998; Ghazanfar and Logothetis, 2003; Izumi and Kojima, 2004), and these visual and auditory channels can be integrated into unified percepts to enhance detection and discrimination. Where and how such communication signals are integrated at the neural level are poorly understood. In particular, it is unclear what role “unimodal” sensory areas, such as the auditory cortex, may play. We recorded local field potential activity, the signal that best correlates with human imaging and event-related potential signals, in both the core and lateral belt regions of the auditory cortex in awake behaving rhesus monkeys while they viewed vocalizing conspecifics. We demonstrate unequivocally that the primate auditory cortex integrates facial and vocal signals through enhancement and suppression of field potentials in both the core and lateral belt regions. The majority of these multisensory responses were specific to face/voice integration, and the lateral belt region shows a greater frequency of multisensory integration than the core region. These multisensory processes in the auditory cortex likely occur via reciprocal interactions with the superior temporal sulcus.
Playing Charades in the fMRI: Are Mirror and/or Mentalizing Areas Involved in Gestural Communication?

Communication is an important aspect of human life, allowing us to powerfully coordinate our behaviour with that of others. Boiled down to its mere essentials, communication entails transferring a mental content from one brain to another. Spoken language obviously plays an important role in communication between human individuals. Manual gestures however often aid the semantic interpretation of the spoken message, and gestures may have played a central role in the earlier evolution of communication. Here we used the social game of charades to investigate the neural basis of gestural communication by having participants produce and interpret meaningful gestures while their brain activity was measured using functional magnetic resonance imaging. While participants decoded observed gestures, the putative mirror neuron system (pMNS: premotor, parietal and posterior mid-temporal cortex), associated with motor simulation, and the temporo-parietal junction (TPJ), associated with mentalizing and agency attribution, were significantly recruited. Of these areas only the pMNS was recruited during the production of gestures. This suggests that gestural communication relies on a combination of simulation and, during decoding, mentalizing/agency attribution brain areas. Comparing the decoding of gestures with a condition in which participants viewed the same gestures with an instruction not to interpret the gestures showed that although parts of the pMNS responded more strongly during active decoding, most of the pMNS and the TPJ did not show such significant task effects. This suggests that the mere observation of gestures recruits most of the system involved in voluntary interpretation.
The Non-lysosomal β-Glucosidase GBA2 Is a Non-integral Membrane-associated Protein at the Endoplasmic Reticulum (ER) and Golgi*

Background: The β-glucosidase GBA2 degrades glucosylceramide (GlcCer) outside the lysosomes. Results: GBA2 is not an integral membrane protein but rather is membrane-associated at the ER and Golgi. Conclusion: GBA2 is located in a key position for a lysosome-independent route of GlcCer-dependent signaling. Significance: Understanding the localization and enzymatic properties of GBA2 is crucial for investigating the role of non-lysosomal glucosylceramide in Gaucher disease pathology. GBA1 and GBA2 are both β-glucosidases, which cleave glucosylceramide (GlcCer) to glucose and ceramide. GlcCer is a main precursor for higher order glycosphingolipids but might also serve as intracellular messenger. Mutations in the lysosomal GBA1 underlie Gaucher disease, the most common lysosomal storage disease in humans. Knocking out the non-lysosomal GBA2 in mice results in accumulation of GlcCer outside the lysosomes in various tissues (e.g. testis and liver) and impairs sperm development and liver regeneration. However, the underlying mechanisms are not well understood. To reveal the physiological function of GBA2 and, thereby, of the non-lysosomal GlcCer pool, it is important to characterize the localization of GBA2 and its activity in different tissues. Thus, we generated GBA2-specific antibodies and developed an assay that discriminates between GBA1 and GBA2 without the use of detergent. We show that GBA2 is not, as previously thought, an integral membrane protein but rather a cytosolic protein that tightly associates with cellular membranes. The interaction with the membrane, in particular with phospholipids, is important for its activity. GBA2 is localized at the ER and Golgi, which puts GBA2 in a key position for a lysosome-independent route of GlcCer-dependent signaling. Furthermore, our results suggest that GBA2 might affect the phenotype of Gaucher disease, because GBA2 activity is reduced in Gba1 knock-out fibroblasts and fibroblasts from a Gaucher patient. Our results provide the basis to understand the mechanism for GBA2 function in vivo and might help to unravel the role of GBA2 during pathogenesis of Gaucher disease.
On-line image guidance for frameless stereotactic radiotherapy of lung malignancies by cone beam CT: Comparison between target localization and alignment on bony anatomy

Introduction. Free-breathing stereotactic radiotherapy for lung malignancies requires reliable prediction of respiratory motion and accurate target localization. A protocol was adopted for reproducibility and reduction of respiratory motion and for target localization by CBCT image guidance. Tumor respiratory displacements and tumor positioning errors relative to bony anatomy alignment are analyzed. Materials and method. Image guided SRT was performed for 99 lung malignancies. Two groups of patients were considered: group A did not perform any breathing control; group B controlled visually their respiratory cycle and volumes on an Active Breathing Coordinator (ABC) monitor during the acquisition of simulation CT and CBCT, and treatment delivery. GTV on end inhale and exhale CT data sets were fused in an ITV and the extent of tumor motion evaluated between these 2 phases. A pre-treatment CBCT was acquired and aligned to the reference CT using bony anatomy; for tumor positioning the ITV contour on the reference CT was matched to the visible tumor on CBCT. Interobserver variability of tumor positioning was evaluated. ITV and CBCT tumor dimensions were compared. Results. 3D tumor breathing displacement (mean±SD) was significantly higher for group A (14.7±9.9 mm) than for group B (4.7±3.1 mm). The detected differences between tumor and bony structure alignment below 3 mm were 68% for group B and 45% for group A, reaching statistical significance. Interobserver variability was 1.7±1.1 mm (mean±SD). Dimensions of tumor image on CBCT were consistent with ITV dimensions for group B (max difference 14%). Conclusions. The adopted protocol seems effective in reducing respiratory internal movements and margin. Tumor positioning errors relative to bony anatomy are also reduced. However bony anatomy as a surrogate of the target may still lead to some relevant positioning errors. Target visualization on CBCT is essential for an accurate localization in lung SRT.