Miracle-dz/UniFAR-FTUs huggingface dataset:
AKR1B10 promotes breast cancer cell migration and invasion via activation of ERK signaling

Background Aldo-keto reductase family 1, member B10 (AKR1B10), is known to be significantly induced in the cells of various cancers such as breast cancer. However, the mechanisms of AKR1B10 promoting tumorigenesis in breast cancer remain unclear. In the present study, we demonstrated the potential role and mechanism of AKR1B10 in the invasion and migration of breast cancer cells. Methods The expression level of AKR1B10 in breast carcinoma, para-carcinoma and cancer tissues were detected by immunohistochemical evaluation and real-time polymerase chain reaction (RT-PCR), and the correlationships between AKR1B10 expression and clinicopathological features in breast cancer patients (n=131) were investigated. AKR1B10 was ectopically expressed in MCF-7 cells or silenced in BT-20 cells. The roles of AKR1B10 expression in the migration and invasion of MCF-7 cells and BT-20 cells were explored by wound healing assay, transwell migration assay and transwell matrigel invasion assay, and finally the activation level of extracellular signal-regulated kinase 1/2 (EKR1/2) activation and the expression level of matrix metalloproteinase-2 (MMP2) and vimentin in MCF-7 and BT-20 cells were measured by western blot. Results We found that AKR1B10 expression was increased in malignant tissues, which was correlated positively with tumor size, lymph node metastasis (p<0.05). MCF-7/AKR1B10 cells displayed a higher ability of migration (43.57±1.04%) compared with MCF-7/vector cells (29.12±1.34%) in wound healing assay, and the migrated cell number of MCF-7/AKR1B10 was more (418.43±9.62) than that of MCF-7/vector (222.43±17.75) in transwell migration assay without matrigel. We furtherly confirmed MCF-7/AKR1B10 cells invaded faster compared with MCF-7/vector cells by transwell matrigel invasion assay. Finally, we found AKR1B10 induced the migration and invasion of MCF-7 and BT-20 cells by activating EKR signaling, which promoted the expressions of MMP2 and vimentin. PD98059, a specific inhibitor of the activation of MEK, blocked the migration and invasion by inhibiting the expression of MMP2 and vimentin. Conclusions AKR1B10 is overexpressed in breast cancer, and promotes the migration and invasion of MCF-7 and BT-20 cells by activating ERK signaling pathway.
Polymedia: Towards a new theory of digital media in interpersonal communication

This article develops a new theory of polymedia in order to understand the consequences of digital media in the context of interpersonal communication. Drawing on illustrative examples from a comparative ethnography of Filipino and Caribbean transnational families, the article develops the contours of a theory of polymedia. We demonstrate how users avail themselves of new media as a communicative environment of affordances rather than as a catalogue of ever proliferating but discrete technologies. As a consequence, with polymedia the primary concern shifts from the constraints imposed by each individual medium to an emphasis upon the social, emotional and moral consequences of choosing between those different media. As the choice of medium acquires communicative intent, navigating the environment of polymedia becomes inextricably linked to the ways in which interpersonal relationships are experienced and managed. Polymedia is ultimately about a new relationship between the social and the technological, rather than merely a shift in the technology itself.
Effects of Expanded California Health Coverage on Hospitals: Implications for ACA Medicaid Expansions.

OBJECTIVE To assess the effects on hospitals of early California actions to expand insurance coverage for low-income uninsured adults after passage of the Affordable Care Act. DATA SOURCES/STUDY SETTING Data from the California Office of Statewide Health Planning and Development and the California Department of Health were merged with U.S. census data for 294 short-term general hospitals during the period 2009-2012. STUDY DESIGN A difference-in-difference analysis was conducted with hospitals in counties that did not implement insurance expansions used as a comparison group. Variables examined included payer mix, costs of unreimbursed care, and hospital operating margin. Sensitivity analyses were conducted as well as a triple difference analysis. Effects were estimated for hospitals overall and by ownership type. PRINCIPAL FINDINGS California insurance expansions primarily benefited for-profit hospitals, with these facilities experiencing significant decreases in self-pay patients, increases in county-covered patients, and reductions in charity care. Most models yielded no significant change in payer mix and conflicting changes in unreimbursed care for nonprofit hospitals. CONCLUSIONS California hospitals that treated the most uninsured prior to insurance expansions did not as a group experience substantial benefit in terms of reduced uninsured burden or better financial performance after program expansions occurred.
Information-motivation-behavioral skills (IMB) model: Testing direct and mediated treatment effects on condom use among women in low-income housing

Background: The Information-Motivation-Behavioral Skills (IMB) model of HIV preventive behavior (1–4) specifies that treatment effects on behavior occur largely as the result of treatment effects on behavioral skills, which follow from effects on information and motivation.Purpose: The objective was to determine whether the variables specified by the IMB model of HIV preventive behavior (1–4) accounted for the relation between an IMB-based treatment and resulting HIV preventive behavior (condom use). Method:Women (n = 557) living in 18 low-income housing developments in 5 geographically dispersed cities were recruited to participate in an HIV-prevention study. Women (within housing developments) were randomly assigned to receive an IMB-based, HIV risk-avoidance intervention or a comparison intervention. Baseline andposttreatment (16 months after baseline) data were collected on condom use information, motivation (social norms, attitudes, intentions, and perceived risk), enactment of behavioral skills (condom negotiation and procurement), and rates of condom use in the past 2 months.Results: The IMB intervention led to a 12% to 16% increase in condom use rates over the course, whereas the comparison intervention led to 2% decrease. In addition, the IMB treatment led to greater increases in condom use information, in the intentions and social norms components of motivation and the condom procurement and condom conversations components of behavioral skills. The IMB model provided an acceptable fit to the data (root mean square error of approximation < .05) and accounted for 50% of the variance inposttreatment condom use among the sample. Treatment effects on condom use were almost entirely mediated by the IMB variables; specifically, motivation and enactment of behavioral skills mediated the intervention’s impact on condom use.Conclusions: These results provide supporting evidence as to how theoretical variables operate to effect change within a theory-based intervention and provide evidence as to the applicability of a prevailing theory of HIV risk behavior among low-income minority women
Cellular and Molecular Biology Cytotoxic effects of new-generation bulk-fill composites on human dental pulp stem cells

: This study was performed to evaluate possible DNA damage in cells of human origin exposed to dental composites in vitro using a cytotoxic assay. Five bulk-fill composites were filled in molds and irradiated for 20 s. DPSCs were inoculated into 24-well plates. After the insert membrane was inserted and composites were added and the experiment was continued for 24/72 hours. In order to investigate the effects of the materials on DPSCs; its effect on apoptosis-re gulating Bcl-2 gene, Human Beta-Defensins (HBDs 1-2) gene, Interleukin 6, 8, 10 expression level was examined. Also in order to check the cellular viability and stress factors; MTT assay, Total Antioxidant and Oxidant Status kits were used. At both irradiation times, all composites significantly affected analyses parameters used in primary DNA damage assessment or induced significant formation of cellular death. Cytotoxicity was detected in TE<SS<FBF<XB<VBF groups at 24 hour, and after 72 hour this sequence has changed.
Conserved but Attenuated Parental Gene Expression in Allopolyploids: Constitutive Zinc Hyperaccumulation in the Allotetraploid Arabidopsis kamchatica

Allopolyploidization combines parental genomes and often confers broader species distribution. However, little is known about parentally transmitted gene expression underlying quantitative traits following allopolyploidization because of the complexity of polyploid genomes. The allopolyploid species Arabidopsis kamchatica is a natural hybrid of the zinc hyperaccumulator Arabidopsis halleri and of the nonaccumulator Arabidopsis lyrata. We found that A. kamchatica retained the ability to hyperaccumulate zinc from A. halleri and grows in soils with both low and high metal content. Hyperaccumulation of zinc by A. kamchatica was reduced to about half of A. halleri, but is 10-fold greater than A. lyrata. Homeologs derived from A. halleri had significantly higher levels of expression of genes such as HEAVY METAL ATPASE4 (HMA4), METAL TRANSPORTER PROTEIN1 and other metal ion transporters than those derived from A. lyrata, which suggests cis-regulatory differences. A. kamchatica has on average about half the expression of these genes compared with A. halleri due to fixed heterozygosity inherent in allopolyploids. Zinc treatment significantly changed the ratios of expression of 1% of homeologous pairs, including genes putatively involved in metal homeostasis. Resequencing data showed a significant reduction in genetic diversity over a large genomic region (290 kb) surrounding the HMA4 locus derived from the A. halleri parent compared with the syntenic A. lyrata-derived region, which suggests different evolutionary histories. We also estimated that three A. halleri-derived HMA4 copies are present in A. kamchatica. Our findings support a transcriptomic model in which environment-related transcriptional patterns of both parents are conserved but attenuated in the allopolyploids.
The impact of artificial selection on morphological integration in the appendicular skeleton of domestic horses

The relationships between the different component parts of organisms, such as the sharing of common development or function, produce a coordinated variation between the different traits. This morphological integration contributes to drive or constrain morphological variation and thus impacts phenotypic diversification. Artificial selection is known to contribute significantly to phenotypic diversification of domestic species. However, little attention has been paid to its potential impact on integration patterns. This study explores the patterns of integration in the limb bones of different horse breeds, using 3D geometric morphometrics. The domestic horse is known to have been strongly impacted by artificial selection, and was often selected for functional traits. Our results confirm that morphological integration among bones within the same limb is strong and apparently partly produced by functional factors. Most importantly, they reveal that artificial selection, which led to the diversification of domestic horses, impacts covariation patterns. The influence of selection on the patterns of covariation varies along the limbs and modulates bone shape, likely due to a differential ligament or muscle development. These results highlight that, in addition to not being constrained by a strong morphological integration, artificial selection has modulated the covariation patterns according to the locomotor specificities of the breeds. More broadly, it illustrates the interest in studying how micro‐evolutionary processes impact covariation patterns and consequently contribute to morphological diversification of domestic species.
Interobserver Consistency of Drug-Induced Sleep Endoscopy in Diagnosing Obstructive Sleep Apnea Using a VOTE Classification System

Objectives: To explore the interobserver consistency of drug-induced sleep endoscopy (DISE) for patients with obstructive sleep apnea syndrome (OSAS) and review the current literature. Methods: In total, 55 patients with an apnea–hypopnea index >5, as determined by on overnight sleep study, were included in this study, 45 males and 10 females, with an average age of 46.87 ± 10.06 years old (range, 19–71). For all OSAS patients, DISE was performed by the same surgeon, which was recorded digitally. The video recordings of DISE were evaluated independently by 3 experienced surgeons who were asked to note his or her decisions as the pattern, site, and degree of upper airway collapse using a VOTE (velum, oropharynx lateral wall, tongue base, and the epiglottis) classification system. Results: Interobserver consistency in the diagnosis of velum-related obstruction in anteroposterior, lateral, and concentric configurations ranged from poor to good. Only significant interobserver consistency among observers A and B was obtained in the diagnosis of oropharynx-related obstruction in the lateral configuration (concordance 60.0%, kappa: 0.365, P < 0.05). Interobserver consistency in the diagnosis of the tongue-related collapse in an anteroposterior configuration, the epiglottis-related collapse in an anteroposterior and lateral configuration ranged from fair to moderate (all kappa values >0.20, all P values < 0.05). Conclusion: Our data suggested that the interobserver consistency of DISE ranged from poor to good. Therefore, further studies with larger numbers of patients are needed to standardize DISE procedures, training, and interpretation.
Development and validation of nomograms for predicting survival in patients with non-metastatic colorectal cancer

Background This study aimed to develop nomograms for predicting survival in patients with non-metastatic colorectal cancer (CRC). Results On multivariate analyses of the derivation set, the nomograms for OS and CSS shared common significant prognostic factors: age, first-degree relative cancer history, differentiation grade, vessels/nerves invasion, TNM stage, CEA, CA19-9 and PNI. The nomograms displayed good accuracy in predicting OS and CSS, with C-indexes of 0.75 and 0.76, respectively. The calibration plots also showed an excellent agreement between the predicted and observed survival probabilities. Furthermore, the predictive accuracy of the nomograms was confirmed in the validation set, with C-indexes of 0.79 and 0.83 for OS and CSS, respectively. Materials and Methods On the basis of data from 822 patients with resected non-metastatic CRC, nomograms for predicting overall survival (OS) and cancer-specific survival (CSS) were established using Cox regression model. The predictive performance of the nomograms was assessed by concordance index (C-index) and calibration plot. An independent external cohort of 171 patients was used to validate the nomograms. Conclusions We developed and validated two nomograms for patients with non-metastatic CRC, which could provide individual prediction of OS and CSS with high accuracy.
Repeated Intravenous Thrombolysis for Early Recurrent Stroke: Challenging the Exclusion Criterion

Background and Purpose— Intravenous thrombolysis (IVT) within 4.5 hours from symptom onset improves functional outcome in patients with acute ischemic stroke. Its use in patients with previous stroke within the preceding 3 months is contraindicated because of the assumed higher risk of intracranial hemorrhage. In addition, tissue-type plasminogen activator may itself promote neurotoxicity and blood–brain barrier disruption. However, safety and effectiveness of repeated IVT is essentially unknown in patients with early (<3 months) recurrent stroke (ERS), because they were excluded from thrombolysis trials. This article reports the largest case series of repeated IVT in ERS. Methods— We reviewed databases of prospectively collected patient data of 8 European stroke centers for the presence of patients with ERS, who received IVT for both the index stroke and ERS. Demographics, clinical and radiological data, bleeding complications, and functional outcome were analyzed. Results— We identified 19 subjects with repeated IVT in ERS. Mean age was 68±12 years, and 37% of them were female. Median interthrombolysis interval was 30 days (interquartile range, 13–50). Functional independence (modified Rankin scale score ⩽2) was achieved in 79% of patients after the first and in 47.4% after repeated IV tissue-type plasminogen activator, respectively. There was no symptomatic intracranial hemorrhage. Median final infarct volume after the first IVT was 1.5 cm3 (interquartile range, 0.5–3.1). Conclusions— Patients with small infarct volumes and robust clinical improvement might be considered for repeated IVT within 3 months. Studies following strict protocols and larger registries incorporating these patients might serve to identify selection criteria for the safe use of repeated IVT in ERS.
Discovery and temporal analysis of latent study patterns in MOOC interaction sequences

Capturing students' behavioral patterns through analysis of sequential interaction logs is an important task in educational data mining and could enable more effective and personalized support during the learning processes. This study aims at discovery and temporal analysis of learners' study patterns in MOOC assessment periods. We propose two different methods to achieve this goal. First, following a hypothesis-driven approach, we identify learners' study patterns based on their interaction with lectures and assignments. Through clustering of study pattern sequences, we capture different longitudinal activity profiles among learners and describe their properties. Second, we propose a temporal clustering pipeline for unsupervised discovery of latent patterns in learners' interaction data. We model and cluster activity sequences at each time step and perform cluster matching to enable tracking learning behaviours over time. Our proposed pipeline is general and applicable in different learning environments such as MOOC and ITS. Moreover, it allows for modeling and temporal analysis of interaction data at different levels of actions granularity and time resolution. We demonstrate the application of this method for detecting latent study patterns in a MOOC course.
Genome composition and phylogeny of microbes predict their co-occurrence in the environment

The genomic information of microbes is a major determinant of their phenotypic properties, yet it is largely unknown to what extent ecological associations between different species can be explained by their genome composition. To bridge this gap, this study introduces two new genome-wide pairwise measures of microbe-microbe interaction. The first (genome content similarity index) quantifies similarity in genome composition between two microbes, while the second (microbe-microbe functional association index) summarizes the topology of a protein functional association network built for a given pair of microbes and quantifies the fraction of network edges crossing organismal boundaries. These new indices are then used to predict co-occurrence between reference genomes from two 16S-based ecological datasets, accounting for phylogenetic relatedness of the taxa. Phylogenetic relatedness was found to be a strong predictor of ecological associations between microbes which explains about 10% of variance in co-occurrence data, but genome composition was found to be a strong predictor as well, it explains up to 4% the variance in co-occurrence when all genomic-based indices are used in combination, even after accounting for evolutionary relationships between the species. On their own, the metrics proposed here explain a larger proportion of variance than previously reported more complex methods that rely on metabolic network comparisons. In summary, results of this study indicate that microbial genomes do indeed contain detectable signal of organismal ecology, and the methods described in the paper can be used to improve mechanistic understanding of microbe-microbe interactions.
The Influence of Fiber Treatment on the Mechanical Behavior of Jute-Coir Reinforced Epoxy Resin Hybrid Composite Plate

A novel surface modification of coir fiber was done by oxidation followed by furfuryl alcohol treatment. The surface modified coir fiber showed better interfacial adhesion with epoxidized phenolic novolac (EPN) thermoset resin compared to unmodified coir fiber reinforced composites. Experimentally, tensile and flexural properties are evaluated at a different percentage of the coir fibers. Stiffness and damping behavior of the composite plates are evaluated theoretically, using a meshless multiquadric radial basis function method. Numerical results are compared with those obtained by other analytical methods. The aim of the study is to observe the behavior of a chemically treated and untreated coir fiber-reinforced composite plate at a different percentage of the coir fibers.
Jaw‐Dropping: Functional Variation in the Digastric Muscle in Bats

Diet and feeding behavior in mammals is strongly linked to the morphology of their feeding apparatus. Cranio‐muscular morphology determines how wide, forcefully, and quickly the jaw can be opened or closed, which limits the size and material properties of the foods that a mammal can eat. Most studies of feeding performance in mammals have focused on skull form and jaw muscles involved in generating bite force, but few explore how jaw abduction is related to feeding performance. In this study, we explored how the morphology of the digastric muscle, the primary jaw abducting muscle in mammals, and its jaw lever mechanics are related to diet in morphologically diverse noctilionoid bats. Results showed that insectivorous bats have strong digastric muscles associated with proportionally long jaws, which suggests these species can open their jaws quickly and powerfully during prey capture and chewing. Short snouted frugivorous bats exhibit traits that would enable them to open their jaws proportionally wider to accommodate the large fruits that they commonly feed on. Our results support the hypothesis that digastric muscle and jaw morphology are correlated with diet in bats, and that our results may also apply to other groups of mammals. Anat Rec, 301:279–290, 2018. © 2018 Wiley Periodicals, Inc.