Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential Expression of Connexins during Neocortical Development and Neuronal Circuit Formation

Gap junctions are membrane channels that mediate the direct passage of ions and molecules between adjacent cells. Recent tracer coupling and optical recording studies have revealed the presence of gap junction-mediated communication between neurons during neocortical development. We have visualized gap junctions in the developing rat cerebral cortex with electron microscopy and studied the pattern of expression and cellular localization of connexins 26, 32, and 43 that take part in their formation. We found that these connexins (Cxs) are expressed differentially during development, and their patterns of expression are correlated with important developmental events such as cell proliferation, migration, and formation of cortical neuronal circuits. Specifically, we observed that the developmental profile of Cx 26 during the first 3 weeks of postnatal life matched closely the development of neuronal coupling, suggesting that coupled neurons use this gap junction protein during circuit formation in the cortex. The subsequent diminution of Cx 26 was mirrored by an increase in Cx 32 immunoreactivity, which became pronounced at the late stages of cortical maturation. In contrast, Cx 43 was localized in the cortex throughout the period of development. Its localization in radial glial fibers closely associated with migrating neurons suggests that this Cx may be involved in neuronal migration.
Long-term efficacy of bosentan in treatment of pulmonary arterial hypertension in children

The aim of the present study was to evaluate a 5-yr experience of bosentan in children with pulmonary arterial hypertension (PAH). A retrospective, observational study was made of children in the UK Pulmonary Hypertension Service for Children (Great Ormond Street Hospital for Children, London, UK) who were given bosentan as monotherapy or in combination, from February 2002 to May 2008 and followed up for ≥6 months. Detailed studies were made of 101 children with idiopathic PAH (IPAH) (n = 42) and PAH associated with congenital heart disease (n = 59). Before treatment, World Health Organization (WHO) functional class, 6-min walk distance (6MWD), height, weight and haemodynamic data were determined. Evaluations were analysed after 6 months and annually to a maximum of 5 yrs. Median duration of treatment was 31.5 months. Initial improvement in WHO functional class and 6MWD was maintained for up to 3 yrs. Height and weight increased but the z-scores did not improve. After 3 yrs, bosentan was continued as monotherapy in only 21% of children with IPAH, but in 69% of repaired cases and 56% of those with Eisenmenger syndrome. The Kaplan–Meier survival estimates for the 101 patients were 96, 89, 83 and 60% at 1, 2, 3 and 5 yrs, respectively. A treatment regime that includes bosentan is safe and appears to be effective in slowing disease progression in children with PAH.
An illustrated key to the genera of Thripinae (Thysanoptera) from South East Asia

An illustrated key is provided for the identification of 65 genera of Thripinae from South East Asia. Wherever possible notes are given on the host-plant associations and geographical distributions, and suggestions made concerning phylogenetic relationships. The combination Taeniothrips euophthalmos Moulton is re-established.
Reliability-aware design to suppress aging

Due to aging, circuit reliability has become extraordinary challenging. Reliability-aware circuit design flows do virtually not exist and even research is in its infancy. In this paper, we propose to bring aging awareness to EDA tool flows based on so-called degradation-aware cell libraries. These libraries include detailed delay information of gates/cells under the impact that aging has on both threshold voltage (Vth) and carrier mobility (μ) of transistors. This is unlike state of the art which considers Vth only. We show how ignoring ß degradation leads to underestimating guard-bands by 19% on average. Our investigation revealed that the impact of aging is strongly dependent on the operating conditions of gates (i.e. input signal slew and output load capacitance), and not solely on the duty cycle of transistors. Neglecting this fact results in employing insufficient guard-bands and thus not sustaining reliability during lifetime. We demonstrate that degradation-aware libraries and tool flows are indispensable for not only accurately estimating guardbands, but also efficiently containing them. By considering aging degradations during logic synthesis, significantly more resilient circuits can be obtained. We further quantify the impact of aging on the degradation of image processing circuits. This goes far beyond investigating aging with respect to path delays solely. We show that in a standard design without any guardbanding, aging leads to unacceptable image quality after just one year. By contrast, if the synthesis tool is provided with the degradation-aware cell library, high image quality is sustained for 10 years (even under worst-case aging and without a guardband). Hence, using our approach, aging can be effectively suppressed.
Disparagement humor and prejudice: Contemporary theory and research

Abstract In this article we review contemporary research testing Martineau’s (1972) hypothesis that disparagement humor fosters or introduces prejudice against the disparaged out-group. Supporting Martineau’s hypothesis, research suggests that instigating disparagement humor might indeed foster prejudice against the targeted group; however, through mechanisms that do not implicate unique effects of humor as a medium for communicating disparagement. Contrary to Martineau’s hypothesis, it does not appear that exposure to disparagement humor promotes a negative disposition toward the targeted group. Rather than acting as an initiator of prejudice, disparagement humor functions as a releaser of existing prejudice. Lastly, following Martineau’s theoretical framework, we identify new questions about the social consequences of disparagement humor that require further theoretical development and empirical research.
Plasma Amino Acid Levels in Huntington's Chorea

Summary Concentrations of plasma amino acids in nine patients with Huntington's chorea and nine control patients were studied while diet and drug therapy were controlled. Significantly low values for threonine, alanine, isoleucine, leucine, lysine and histidine were found in the Huntington's chorea patients. However, since different investigators have failed to establish a consistent pattern of abnormality, it is considered that the findings are probably due to non-specific factors.
Fruit and vegetable intake and the risk of gastric adenocarcinoma: A reanalysis of the european prospective investigation into cancer and nutrition (EPIC‐EURGAST) study after a longer follow‐up

In a previous European prospective investigation into cancer and nutrition (EPIC) analysis, we found an inverse association between total intake of vegetables, onion and garlic, and risk of intestinal gastric cancer (GC) and between citrus fruit and risk of cardia GC. The aim of this study is to reanalyze the effect of fruit and vegetables (F&V), based on a longer follow‐up and twice the number of GC cases. Subjects are 477,312 men and women mostly aged 35 to 70 years participating in the EPIC cohort, including 683 gastric adenocarcinomas with 11 years of follow‐up. Information on diet and lifestyle was collected at baseline. A calibration study in a subsample was used to correct for dietary measurement errors. When comparing the highest vs. lowest quintile of intake, we found an inverse association between total intake of V&F and GC risk [hazard ratio (HR) 0.77; 95% confidence interval (CI) 0.57–1.04; p for trend 0.02], between fresh fruit and risk of the diffuse type (HR 0.59; 95% CI 0.36–0.97; p for trend 0.03) and an inverse association between citrus fruit and risk of cardia cancer (HR 0.61; 95% CI 0.38–1.00, p for trend 0.01). Although calibration revealed somewhat stronger inverse associations, none of the risks reached statistical significance. There was no association between total or specific vegetables intake and GC risk. The inverse association between fresh fruit and citrus fruits and risk of GC seems to be restricted to smokers and the Northern European countries. Fresh fruit and citrus fruit consumption may protect against diffuse and cardia GC, respectively.
Reversal of Myeloid Cell–Mediated Immunosuppression in Patients with Metastatic Renal Cell Carcinoma

Purpose: Tumor-induced immunosuppression remains a significant obstacle that limits the efficacy of biological therapy for renal cell carcinoma. Here we evaluate the role of CD33 myeloid-derived suppressor cells (MDSC) in the regulation of T-cell responses in renal cell carcinoma patients. We also examine effect of all-trans-retinoic acid (ATRA) on MDSC-mediated immune suppression. Experimental Design: CD33-positive myeloid cells were isolated from the peripheral blood of renal cell carcinoma patients with magnetic beads and tested in vitro for their ability to inhibit T-cell responses. T-cell function was evaluated using ELISPOT and CTL assays. Results: MDSC isolated from renal cell carcinoma patients, but not from healthy donors, were capable of suppressing antigen-specific T-cell responses in vitro through the secretion of reactive oxygen species and nitric oxide upon interaction with CTL. MDSC-mediated immune suppression and IFN-γ down-regulation was reversible in vitro by exposing cells to the reactive oxygen species inhibitors. Moreover, ATRA was capable of abrogating MDSC-mediated immunosuppression and improving T-cell function by direct differentiation into antigen-presenting cell precursors. Conclusions: These results may have significant implications regarding the future design of active immunotherapy protocols that may include differentiation agents as part of a multimodal approach to renal cell carcinoma immunotherapy.
The Entrepreneurial Intention of University Students: The Case of a University of Technology in South Africa

This quantitative study was executed from a realist‘s ontological perspective and its epistemological leaning is towards that of an empiricist. The study essentially sought to determine the existence or otherwise of entrepreneurial intentions among the students. Ample emphasis needs to be placed on entrepreneurship education and practical entrepreneurship schemes (such as mentorship programmes) if developing countries are to realise the goal of having a productive and virile youth population, which would represent a significant shift from today‘s yawning youth unemployment position. The study collected data in a cross-sectional manner from a random sample of 150 students drawn from a leading South African University of Technology. In analyzing the data, there was recourse to the use of descriptive as well as inferential statistics. Interestingly, results show no statistically significant relationships between students‘ entrepreneurial intention and selected sociodemographic variables such as age, gender, culture, etc. While we acknowledge that the results of this study emerged from a sample of 150 students of a particular university and therefore betray the concept of generalization, we are equally confident that the findings have significant implications for developing economies around the world including South Africa.
Unzipping the Role of Myosin Light Chain Phosphatase in Smooth Muscle Cell Relaxation*

Recently, it has been hypothesized that myosin light chain (MLC) phosphatase is activated by cGMP-dependent protein kinase (PKG) via a leucine zipper-leucine zipper (LZ-LZ) interaction through the C-terminal LZ in the myosin-binding subunit (MBS) of MLC phosphatase and the N-terminal LZ of PKG (Surks, H. K., Mochizuki, N., Kasai, Y., Georgescu, S. P., Tang, K. M., Ito, M., Lincoln, T. M., and Mendelsohn, M. E. (1999) Science 286, 1583-1587). Alternative splicing of a 3′-exon produces a LZ+ or LZ- MBS, and the sensitivity to cGMP-mediated smooth muscle relaxation correlates with the relative expression of LZ+/LZ- MBS isoforms (Khatri, J. J., Joyce, K. M., Brozovich, F. V., and Fisher, S. A. (2001) J. Biol. Chem. 276, 37250 -37257). In the present study, we determined the effect of LZ+/LZ- MBS isoforms on cGMP-induced MLC20 dephosphorylation. Four avian smooth muscle MBS-recombinant adenoviruses were prepared and transfected into cultured embryonic chicken gizzard smooth muscle cells. The expressed exogenous MBS isoforms were shown to replace the endogenous isoform in the MLC phosphatase holoenzyme. The interaction of type I PKG (PKGI) with the MBS did not depend on the presence of cGMP or the MBS LZ. However, direct activation of PKGI by 8-bromo-cGMP produced a dose-dependent decrease in MLC20 phosphorylation (p < 0.05) only in smooth muscle cells expressing a LZ+ MBS. These results suggest that the activation of MLC phosphatase by PKGI requires a LZ+ MBS, but the binding of PKGI to the MBS is not mediated by a LZ-LZ interaction. Thus, the relative expression of LZ+/LZ- MBS isoforms could explain differences in tissue sensitivity to NO-mediated vasodilatation.
Foreign Branding and Its Effects on Product Perceptions and Attitudes

With three experiments, the authors examine the notion that foreign branding—the strategy of pronouncing or spelling a brand name in a foreign language—triggers cultural stereotypes and influences product perceptions and attitudes. Choosing French brands as one specific case, Experiment 1 shows that the French pronunciation of a brand name affects the perceived hedonism of the products, attitudes toward the brand, and attitudes toward the brand name. Experiment 2 shows that congruent country-of-origin information, added to French branding, does not result in more hedonic perceptions; incongruence, however, diminishes the effect. In Experiment 3, an actual product taste test is performed. Despite the presence of direct sensory experience, consumer perceptions of a product change as a result of French branding.
Improving Hospital Discharge Planning for Elderly Patients

Hospital discharge planning has become increasingly important in an era of prospective payment and managed care. Given the changes in tasks, decisions, and environments involved, it is important to identify how to move such planning from an art to an empirically based decisionmaking process. The authors use a decision-sciences framework to review the state-of-the-art of hospital discharge planning and to suggest methods for improvement.
Acute arsenic toxicity alters cytochrome P450 and soluble epoxide hydrolase and their associated arachidonic acid metabolism in C57Bl/6 mouse heart

Acute arsenic (As(III)) exposure has been reported to cause cardiac toxicity, however this toxicity was never linked to the disturbance in cytochrome P450 (P450)-mediated arachidonic acid metabolism. Therefore, we investigated the effect of acute As(III) toxicity on the expression of P450 and soluble epoxide hydrolase (sEH) and their associated arachidonic acid metabolism in mice hearts. As(III) toxicity was induced by a single intraperitoneal injection of 12.5 mg/kg of As(III). Our results showed that As(III) treatment caused a significant induction of the cardiac hypertrophic markers in addition to Cyp1b1, Cyp2b, Cyp2c, Cyp4f, and sEH gene expression in mice hearts. Furthermore, As(III) increased sEH protein expression and activity in hearts with a consequent decrease in 11,12-, and 14,15-epoxyeicosatrienoic acids (EETs) formation. Whereas the formation of 8,9-, 11,12-, 14,15-dihydroxyeicosatrienoic acids (DHETs) was significantly increased. As(III) also increased sEH mRNA and protein expression levels in addition to the hypertrophic markers which was reversed by knockdown of sEH in H9c2 cells. In conclusion, acute As(III) toxicity alters the expression of several P450s and sEH enzymes with a consequent decrease in the cardioprotective EETs which may represent a novel mechanism by which As(III) causes progressive cardiotoxicity. Furthermore, inhibiting sEH might represent a novel therapeutic approach to prevent As(III)-induced hypertrophy.
Les minima sociaux en France: entre compensation et responsabilité

[fre] Les minima sociaux en France : entre compensation et responsabilite par Marc Fleurbaey, Cyrille Hagnere, Michel Martinez et Alain Trannoy . Cet article propose une grille de lecture d'un systeme de transferts a partir de la notion de responsabilite individuelle : si le montant d'une aide quelconque est independant des caracteristiques individuelles des beneficiaires, ces derniers sont implicitement tenus pour responsables de ces caracteristiques. Apres l'expose du cadre theorique, nous passons en revue l'ensemble des aides destinees aux personnes defavorisees, en examinant successivement la prise en compte de caracteristiques socio-demographiques, et celles des revenus d'activite. Cette approche se revele utile, en particuUer, pour mettre en lumiere des hypotheses implicites un peu bizarres quant aux normes de comportement en matieres d'aides inter-individuelles. Un traitement avantageux des familles monoparentales bien que mal adapte semble egalement emerger. D ' autre part, la prise en compte de la structure dynamique des versements des allocations permet de montrer que la reforme Aubry des minima sociaux a eu un effet non negligeable dans la diminution des trappes a inactivite. [eng] Income Support in France: Between Compensation and Responsibility by Marc Fleurbaey, Cyrille Hagnere, Michel Martinez and Alain Trannoy . This paper suggests a reading grid for a system of transfers based on the notion of individual responsibility: if the sum of a given benefit is independent of the individual characteristics of the recipients, these recipients are implicitly taken to be responsible for these characteristics. We first present the theoretical framework and then review all the benefits for underprivileged individuals, studying how socio-demographic and earned-income characteristics are taken into account. This approach is useful, in particular, to bring to light slightly unusual implicit assumptions as regards inter-individual assistance behavioural norms. A favourable, albeit poorly suited, treatment of lone-parent families also emerges. Moreover, consideration of the dynamic structure of benefit payments shows that the Aubry reform of income support (i.e.Specific Solidarity Allowance[ASS],Integration Allowance [AI],Minimum Integration Income [RMI], Single Parent Allowance [API] and Widow's Benefit) has had a not-inconsiderable effect on the decrease in unemployment traps.
Pre-Enrollment Considerations of Undergraduate Wheelchair Users and Their Post-Enrollment Transitions.

The purpose of this study was to examine the pre-enrollment considerations of undergraduate wheelchair users (i.e., the decision to attend college, college selection factors) and their post-enrollment transitions (i.e., adjustments from high school to college, academic and social integration). Qualitative ethnographic research methodology was used to reveal the voices of 10 students using wheelchairs and four of their parents. The determining factors in the college selection process for students using wheelchairs were the academic majors available at the university, coupled with the physical accessibility of the campus and a strong office of disability services. The freedom that is attached to making personal decisions and friends was a new experience to many of them and, after a brief transitional time, they relished it. From being able to get around on their own to and from class, to hanging out with friends, a feeling of independence was the key to integrating into college. Similar to peers not in wheelchairs, these students had learned how to navigate the higher education setting and be vocal about their needs (i.e., self-advocate). A series of recommendations for students in wheelchairs, their parents, and colleges and universities is provided.
Ring-Theoretic Properties of Certain Hecke Algebras

The purpose of this article is to provide a key ingredient of [W2] by establishing that certain minimal Hecke algebras considered there are complete intersections. As is recorded in [W2], a method going back to Mazur [M] allows one to show that these algebras are Gorenstein, but for the complete intersection property a new approach is required. The methods of this paper are related to those of Chapter 3 of [W2]. The methods of Section 3 of this paper are based on a previous approach of one of us (A.W.). We would like to thank Henri Darmon, Fred Diamond and Gerd Faltings for carefully reading the first version of this article. Gerd Faltings has also suggested a simplification of our argument as well as of the argument of Chapter 3 of [W2] and we would like to thank him for allowing us to reproduce these in the appendix to this paper. R. T. would like to thank A. W. for his invitation to collaborate and for sharing his many insights into the questions considered. R. T. would also like to thank Princeton University, Universite de Paris 7 and Harvard University for their hospitality during this collaboration. A. W. was supported by an NSF grant.
Phenotype-based high-content chemical library screening identifies statins as inhibitors of in vivo lymphangiogenesis

Lymphangiogenesis plays an important role in promoting cancer metastasis to sentinel lymph nodes and beyond and also promotes organ transplant rejection. We used human lymphatic endothelial cells to establish a reliable three-dimensional lymphangiogenic sprouting assay with automated image acquisition and analysis for inhibitor screening. This high-content phenotype-based assay quantifies sprouts by automated fluorescence microscopy and newly developed analysis software. We identified signaling pathways involved in lymphangiogenic sprouting by screening the Library of Pharmacologically Active Compounds (LOPAC)1280 collection of pharmacologically relevant compounds. Hit characterization revealed that mitogen-activated protein kinase kinase (MEK) 1/2 inhibitors substantially block lymphangiogenesis in vitro and in vivo. Importantly, the drug class of statins, for the first time, emerged as potent inhibitors of lymphangiogenic sprouting in vitro and of corneal and cutaneous lymphangiogenesis in vivo. This effect was mediated by inhibition of the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase and subsequently the isoprenylation of Rac1. Supplementation with the enzymatic products of HMG-CoA reductase functionally rescued lymphangiogenic sprouting and the recruitment of Rac1 to the plasma membrane.