Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of carbon to nitrogen ratios on soybean somatic embryo (cv. Jack) growth and composition

Soybean [Glycine max (L.) Merr. ] seed are valued for their protein and oil content. Soybean somatic embryos cultured in Soybean Histodifferentiation and Maturation (SHaM) medium were examined for their suitability as a model system for developing an understanding of assimilate partitioning and metabolic control points for protein and oil biosynthesis in soybean seed. This report describes the growth dynamics and compositional changes of SHaM embryos in response to change in the carbon to nitrogen ratio of the medium. It was postulated that at media compositions that were sufficient to support maximal growth rates, changes in the C:N ratio are likely to influence the partitioning of resources between the various storage products, especially protein and oil. As postulated, at steady-state growth rates, embryo protein content was strongly correlated with decreasing C:N ratios and increasing glutamine consumption rates. However, oil content remained relatively unchanged across the C:N ratio range tested, and resources were instead directed towards the starch and residual biomass (estimated by mass balance) pools in response to increasing C:N ratios. Protein and oil were inversely related only at concentrations of sucrose in the medium <88mM, where carbon limited growth and no starch was found to accumulate in the tissues. These observations and the high reproducibility in the data indicate that SHaM embryos are an ideal model system for the application of metabolic flux analysis studies designed to test hypotheses regarding assimilate partitioning in developing soybean seeds.
The influence of sediment characteristics on the fate of steroidogenic compounds in aquatic systems and the effects on progesterone bioavailability in a target organism

There is growing concern about the biologic effects stemming from steroids in impacted waterways. In aquatic systems, interaction between steroids and sediment influence both contaminant fate as well as subsequent bioavailability to aquatic organisms. The focus of this dissertation research was to gain a better understanding of steroid behavior in aquatic systems based on the physiochemical properties of sediment and to use this knowledge to better understand the biological effects stemming from sediment-associated progesterone exposure. Two natural aquatic sediments, a sand and a silty loam, were selected to represent marked differences in sediments properties. Initially, sorption of 17β-estradiol, estrone, progesterone, and testosterone was evaluated to different size fractions of each sediment to determine the steroid sorption capacity and distribution within the whole sediment. Sorption capacity was influenced more by organic carbon content than particle size; while, interactions between size fractions were found to affect the distribution of steroids within the whole sediments. In a subsequent study, the sediments were used to evaluate the fate of progesterone and the corresponding alteration of gene expression in a target organism, the fathead minnow (Pimephales promelas), using three steroid-responsive genes; vitellogenin, androgen receptor and estrogen receptor-alpha. When exposed to progesterone spiked sand, fish exhibited significant reductions in the expression of vitellogenin in the 5 and 50 ng/g treatment groups at 7 d and significant reductions in vitellogenin and androgen receptor expression after 14 d in the 50 and 500 ng/g treatment groups. In contrast, fish exposed to progesterone associated with the silty loam sediments did not show a biological response at 7 d and only realized a significant reduction in vitellogenin at 14 d in the 50 and 500 ng/g groups. In both sediments, progesterone degradation resulted in the production of androgens including androsteinedione, testosterone, and androstadienedione, as well as the anti-estrogen, testolactone. Differences in compound fate resulted in organism exposure to different suites of metabolites either in water or associated with the sediment. Results from this study suggest that environmental progestagens will lead to defeminization at environmentally-relevant concentrations, and that exposure is influenced by sediment properties. Sources of steroids in the environment 1 Fate of steroidogenic compounds in agricultural soils 3 Effects of particle size on steroid sorption 4 Microbial degradation of steroids 5 Fate of steroidogenic compounds in aquatic systems 7 Biological effects of aqueous steroids 8 Biological effects of aqueous progesterone 9 Biological effects of sediment-associated steroids 11
Associations between adult attachment dimensions and attitudes toward pain behaviour.

BACKGROUND Despite the important role positive reinforcement of pain behaviour is believed to play in chronic pain, there is a paucity of research regarding factors that influence the provision of such reinforcement. Attachment theory suggests that individuals high in attachment avoidance view the pain behaviour of others in a negative manner and would, therefore, provide little reinforcement of pain behaviour. As an initial step in evaluating this model, relationships between attachment dimensions and attitudes toward pain behaviour were examined. Attachment avoidance was hypothesized to be negatively associated with accepting attitudes toward pain behaviour. METHODS A sample of undergraduate students (n=160) completed the Relationships Structures Questionnaire, which provides global ratings of adult attachment dimensions (anxiety and avoidance) by assessing attachment across four relationship targets (friend, mother, father and romantic partner). Attitudes regarding the acceptability of pain behaviour were assessed using male and female versions of the Appropriate Pain Behaviour Questionnaire (APBQ). RESULTS Consistent with the hypothesis, attachment avoidance was negatively correlated with both APBQ-Female and APBQ-Male scores. Multiple regression analyses were used to investigate the relationships between the attachment scales and the APBQ scales while statistically adjusting for sex and testing for interaction effects. The findings revealed complex relationships involving interaction effects that provided further support for the hypothesis. CONCLUSIONS The findings provided support for the hypothesis that attachment avoidance is associated with less accepting attitudes toward pain behaviour. Additional research regarding the role of attachment and attitudes on responses to pain behaviour is warranted.
Clinical and laboratory indices that enhance the diagnosis of postural tachycardia syndrome.

OBJECTIVE To identify clinical and laboratory indices that improve the diagnosis of the postural tachycardia syndrome (POTS). DESIGN We assessed associations of orthostatic intolerance by using multivariate regression analysis. MATERIAL AND METHODS We evaluated autonomic symptoms and autonomic function in 30 patients with POTS, 30 patients with mild orthostatic intolerance, and 19 age- and gender-matched control subjects. Indices of parasympathetic and sympathetic functions were analyzed on the basis of (1) autonomic function tests (head-up tilt), (2) oscillations at respiratory and nonrespiratory frequencies (0.01 to 0.09 Hz) in R-R interval and blood pressure (Wigner distribution), and (3) deterministic component (rescaled range analysis). RESULTS The four clinical and laboratory indices that independently supported the diagnosis of POTS are as follows: (1) orthostatic heart rate during the first minute of head-up tilt, (2) autonomic deficit (adrenergic autonomic score), (3) loss of spectral powers in R-R interval during head-up tilt at the fifth minute, and (4) severity of orthostatic dizziness, fatigue, palpitations, and shortness of breath. CONCLUSION Enhancing the sensitivity and specificity of the diagnosis of POTS should be possible by using these four indices. A hyperadrenergic state and distal neuropathy, affecting adrenergic sympathetic cardiovagal fibers, seem to be involved in the pathophysiology of POTS. Certain features suggest brain-stem dysregulation.
Repair of ventral root avulsions of the brachial plexus: a review

Abstract Traumatic root avulsions of the brachial plexus constitute a devastating lesion resulting in loss of function of the upper limb and carry a large emotional and socioeconomic impact. In this literature survey, the different factors involved in root avulsion are discussed in combination with various surgical techniques for repair of experimental ventral root avulsion. Until now repair of root avulsions did not generate unequivocal proof of recovery of limb function, particularly of the hand. More experimental studies are needed to assess the efficacy of several repair techniques, the optimal timing for surgery, and the complications associated with spinal cord manipulation.
RIG-I-Mediated Antiviral Signaling Is Inhibited in HIV-1 Infection by a Protease-Mediated Sequestration of RIG-I

ABSTRACT The rapid induction of type I interferon (IFN) is essential for establishing innate antiviral responses. During infection, cytoplasmic viral RNA is sensed by two DExD/H box RNA helicases, RIG-I and MDA5, ultimately driving IFN production. Here, we demonstrate that purified genomic RNA from HIV-1 induces a RIG-I-dependent type I IFN response. Both the dimeric and monomeric forms of HIV-1 were sensed by RIG-I, but not MDA5, with monomeric RNA, usually found in defective HIV-1 particles, acting as a better inducer of IFN than dimeric RNA. However, despite the presence of HIV-1 RNA in the de novo infection of monocyte-derived macrophages, HIV-1 replication did not lead to a substantial induction of IFN signaling. We demonstrate the existence of an evasion mechanism based on the inhibition of the RIG-I sensor through the action of the HIV-1 protease (PR). Indeed, the ectopic expression of PR resulted in the inhibition of IFN regulatory factor 3 (IRF-3) phosphorylation and decreased expression of IFN and interferon-stimulated genes. A downregulation of cytoplasmic RIG-I levels occurred in cells undergoing a single-cycle infection with wild-type provirus BH10 but not in cells transfected with a protease-deficient provirus, BH10-PR−. Cellular fractionation and confocal microscopy studies revealed that RIG-I translocated from the cytosol to an insoluble fraction during the de novo HIV-1 infection of monocyte-derived macrophages, in the presence of PR. The loss of cytoplasmic RIG-I was prevented by the lysosomal inhibitor E64, suggesting that PR targets RIG-I to the lysosomes. This study reveals a novel PR-dependent mechanism employed by HIV-1 to counteract the early IFN response to viral RNA in infected cells.
Large-scale multimodal semantic concept detection for consumer video

In this paper we present a systematic study of automatic classification of consumer videos into a large set of diverse semantic concept classes, which have been carefully selected based on user studies and extensively annotated over 1300+ videos from real users. Our goals are to assess the state of the art of multimedia analytics (including both audio and visual analysis) in consumer video classification and to discover new research opportunities. We investigated several statistical approaches built upon global/local visual features, audio features, and audio-visual combinations. Three multi-modal fusion frameworks (ensemble, context fusion, and joint boosting) are also evaluated. Experiment results show that visual and audio models perform best for different sets of concepts. Both provide significant contributions to multimodal fusion, via expansion of the classifier pool for context fusion and the feature bases for feature sharing. The fused multimodal models are shown to significantly reduce the detection errors (compared to single modality models), resulting in a promising accuracy of 83% over diverse concepts. To the best of our knowledge, this is the first work on systematic investigation of multimodal classification using a large-scale ontology and realistic video corpus.
Influence of PA6 as a Charring Agent on Flame Retardancy, Thermal and Mechanical Properties of LGFR PP Composites

Abstract In this work, the combustion, thermal, and mechanical performances of long-glass-fiber-reinforced polypropylene/intumescent flame retardant (LGFPP/IFR) composites with different contents of polyamide 6 (PA6) as a charring agent were investigated by limiting oxygen index (LOI), UL-94 test, cone calorimetry, thermal gravimetric analysis (TGA), scanning electron microscopy (SEM), and mechanical property test. The results showed that LGFPP/IFR/PA6 composites exhibited much better flame retardancy due to the dense char layer structure, as proved by SEM. When 15 wt% PA6 was added, the LGFPP/IFR composites had the best flame retardancy, and LOI increased by 55.1 %, and the UL-94 reached V-0 rating. The cone calorimeter tests indicated that PA6 could prevent the transmission of heat and the volatilization of flammable substances, thereby reducing the heat release of “second burning”. According to TGA analysis, the carbon layer began to form at a lower temperature due to the synergistic effect between IFR and PA6. Moreover, the calculation of apparent active energy (Ea) revealed that PA6 effectively increased the Ea values of the composites, resulting in a better thermal stability and flame retardancy. In addition, PA6 enhanced the mechanical properties of the composites effectively.
Inhibition of Excitatory Synaptic Transmission in Hippocampal Neurons by Levetiracetam Involves Zn2+-Dependent GABA Type A Receptor–Mediated Presynaptic Modulation

Levetiracetam (LEV) is an antiepileptic drug with a unique but as yet not fully resolved mechanism of action. Therefore, by use of a simplified rat-isolated nerve-bouton preparation, we have investigated how LEV modulates glutamatergic transmission from mossy fiber terminals to hippocampal CA3 neurons. Action potential–evoked excitatory postsynaptic currents (eEPSCs) were recorded using a conventional whole-cell patch-clamp recording configuration in voltage-clamp mode. The antiepileptic drug phenytoin decreased glutamatergic eEPSCs in a concentration-dependent fashion by inhibiting voltage-dependent Na+ and Ca2+ channel currents. In contrast, LEV had no effect on eEPSCs or voltage-dependent Na+ or Ca2+ channel currents. Activation of presynaptic GABA type A (GABAA) receptors by muscimol induced presynaptic inhibition of eEPSCs, resulting from depolarization block. Low concentrations of Zn2+, which had no effect on eEPSCs, voltage-dependent Na+ or Ca2+ channel currents, or glutamate receptor–mediated whole cell currents, reduced the muscimol-induced presynaptic inhibition. LEV applied in the continuous presence of 1 µM muscimol and 1 µM Zn2+ reversed this Zn2+ modulation on eEPSCs. The antagonizing effect of LEV on Zn2+-induced presynaptic GABAA receptor inhibition was also observed with the Zn2+ chelators Ca-EDTA and RhodZin-3. Our results clearly show that LEV removes the Zn2+-induced suppression of GABAA-mediated presynaptic inhibition, resulting in a presynaptic decrease in glutamate-mediated excitatory transmission. Our results provide a novel mechanism by which LEV may inhibit neuronal activity.
High-speed InP/InGaAs uni-traveling-carrier photodiodes with 3-dB bandwidth over 150 GHz

High-speed and high-saturation-power photodetectors operating at 1.3 and 1.55 /spl mu/m wavelength are attracting considerable attention for the purpose of configuring receivers with no broadband electronic post-amplifier. The uni-traveling-carrier photodiode (UTCPD) having a p-type photo-absorption layer and a widegap electron-collection layer is one candidate that fulfills the requirements. In this paper, we study photo-absorption layer design for faster response and demonstrate UTC-PD operations with 3-dB bandwidth of 152 GHz.
Fluid integration of rotation and translation

Previous research has shown that rotation and orientation of items plays three major roles during collaboration: comprehension, coordination and communication. Based on these roles of orientation and advice from kinesiology research, we have designed the Rotate'N Translate (RNT) interaction mechanism, which provides integrated control of rotation and translation using only a single touch-point for input. We present an empirical evaluation comparing RNT to a common rotation mechanism that separates control of rotation and translation. Results of this study indicate RNT is more efficient than the separate mechanism and better supports the comprehension, coordination and communication roles of orientation.
Peroxisome Proliferator-Activated Receptor δ: A Target with a Broad Therapeutic Potential for Drug Discovery

The biology of Peroxisome proliferator-activated receptor (PPAR) alpha (α) and gamma (γ) has been intensely scrutinized for the last 20 years and the clinical use of both PPAR-α (fibrates) and PPAR-γ (thiazolididiones) agonists has led to the understanding of their key role in the treatment of hypertriglyceridemia and type 2 diabetes mellitus [1, 2]. In contrast, the understanding of PPAR delta (δ) biology still lags behind. The identification of small molecule agonists for PPAR-δ has shed some light on the function of this ubiquitously expressed receptor in preclinical models and early clinical studies [3]. They have revealed the multiple benefits of PPAR-δ activation on lipid disorders, diabetes, and inflammation [3, 4]. However, synthetic PPAR-δ agonists have yet to be marketed for clinical use in humans, partly due to the burden associated with their clinical development [3].
Effects of Domain, Retention Interval, and Information Load on Young and Older Adults’ Visuospatial Working Memory

Age-related differences in visuospatial working memory were examined in 69 young adults and 49 older adults exposed to three pairs of tasks. Each pair consisted of one task involving information about the form or appearance of items and another task involving information about item locations. The first pair of tasks manipulated retention interval and required maintaining information about one item. The second pair also manipulated retention interval and required maintaining information about multiple items presented simultaneously. The third pair manipulated the number of sequentially presented items. Analyses of the first two pairs of tasks revealed significant age deficits in working memory for spatial locations but not in working memory for visual features. Notably, there were no age differences in the effect of retention interval on any of the four tasks, suggesting that visuospatial information is lost at similar rates in older and young adults. Analyses of the third pair of tasks revealed that, regardless of domain, increasing the amount of information impaired older adults’ memory performance to a greater extent than young adults’ performance. Thus, the present results suggest differences in basic working memory capacity in both domains, but a lack of age differences in rates of forgetting from working memory, and greater age-related deficits in the spatial domain than in the object domain.
HIV coreceptor and chemokine ligand gene expression in the male urethra and female cervix

Objective:Isolates with a tropism for the coreceptor CCR5 are the predominant viral strain transmitted following heterosexual transmission. We have investigated coreceptor expression levels within male and female genital epithelia to assess whether selective transmission can be explained by elevated CCR5 expression within the genital epithelia per se. Design:Individuals attending a local genitourinary medicine unit were recruited, and samples of genital epithelia obtained using either a cytobrush (females) or urethral swab (males). Expression of coreceptor and cell marker mRNAs was then determined by reverse transcription (RT)–PCR. Methods:RNA was recovered from the epithelial cell samples then used as templates in competitive quantitative RT–PCR to measure mRNA expression of key chemokines, coreceptors and cell-type markers in the epithelial cell samples. Cell-surface coreceptor expression was also assessed in a sample of patients using fluorescent cell staining. Results:CXCR4 and CCR3 coreceptors were expressed at significantly higher levels than CCR5 within the female endo- and ectocervix and distal end of the male urethra. Increased levels of cell surface expressed CXCR4 compared to CCR5 was confirmed in samples obtained from the female genital tract by FACS analysis. Conclusions:The selective transmission of CCR5-tropic viral variants is unlikely to result simply from differential coreceptor abundance at the genital epithelia.
New Genotypes of Orientia tsutsugamushi Isolated from Humans in Eastern Taiwan

Scrub typhus, an acute febrile illness, is caused by the obligate intracellular bacterium Orientia tsutsugamushi. In our study, O. tsutsugamushi was rapidly detected and typed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 56-kDa type-specific antigen (TSA) gene. To investigate the genotypes of clinical variants of O. tsutsugamushi, we collected 3223 blood samples from eastern Taiwanese patients with suspected scrub typhus from 2002 to 2008. In total, 505 samples were found to be positive for scrub typhus infection by PCR, and bacteria were isolated from 282 of them. Four prototype genotype strains (Karp, Kato, Kawasaki and Gilliam) and eleven different Taiwanese genotype isolates (Taiwan-A, -B, -C, -D, -E, -G, -H, -J, -N, -O and -P) were identified by RPLF analysis. Taiwan-H, the major genotype in eastern Taiwan, exhibited prevalence and isolation rates of 47.3% (239/505) and 42.6% (120/282), respectively. We also assessed the genetic relatedness of the 56-kDa TSA gene among eight Taiwan-H isolates, thirteen other Taiwanese isolates and 104 DNA sequences deposited in the GenBank database using MEGA version 5.0 and PHYLIP version 3.66. We found that the Taiwan-H isolates formed into a new cluster, which was designated the Taiwan Gilliam-variant (TG-v) cluster to distinguish it from the Japanese Gilliam-variant (JG-v) cluster. According to Simplot analysis, TG-v is a new recombinant strain among Gilliam, Ikeda and Kato. Moreover, the Gilliam-Kawasaki cluster had the highest percentage of RFLP cases and was the most frequently isolated type in eastern Taiwan (50.1%, 253/505; 44.0%, 124/282). These findings shed light on the genetic evolution of O. tsutsugamushi into different strains and may be useful in vaccine development and epidemic disease control in the future.