Miracle-dz/UniFAR-FTUs huggingface dataset:
Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation Pattern Induction

Background: MSCs are a promising therapeutic resource. Paracrine effects and the induction of differentiation patterns are thought to represent the two primary mechanisms underlying the therapeutic effects of mesenchymal stem cell (MSC) transplantation in vivo. However, it is unclear which mechanism is involved in the therapeutic effects of human umbilical cord-derived MSC (hUC-MSC) transplantation. Methods and Results: Based on flow cytometry analysis, hUC-MSCs exhibited the morphological characteristics and surface markers of MSCs. Following directed neural induction, these cells displayed a neuron-like morphology and expressed high levels of neural markers. All types of hUC-MSCs, including differentiated and redifferentiated cells, promoted learning and memory function recovery in hypoxic-ischemic brain damaged (HIBD) rats. The hUC-MSCs secreted IL-8, which enhanced angiogenesis in the hippocampus via the JNK pathway. However, the differentiated and redifferentiated cells did not exert significantly greater therapeutic effects than the undifferentiated hUC-MSCs. Conclusion: hUC-MSCs display the biological properties and neural differentiation potential of MSCs and provide therapeutic advantages by secreting IL-8, which participates in angiogenesis in the rat HIBD model. These data suggest that hUC-MSC transplantation improves the recovery of neuronal function via an IL-8-mediated secretion mechanism, whereas differentiation pattern induction was limited.
Inflammasome activation causes dual recruitment of NLRC4 and NLRP3 to the same macromolecular complex

Significance The nucleotide-binding oligomerization domain-like receptor (NLR) family members, NLRC4 and NLRP3, activate the inflammasome to provide host defenses against infection. The precise molecular constituents of an inflammasome are unknown; however, it is believed that receptor-specific complexes containing apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) and caspase-1 are formed. Here, we used confocal and superresolution microscopy to show that in macrophages infected with Salmonella Typhimurium, a pathogen that activates two distinct NLRs, ASC forms an outer ring-like structure that comprises NLRC4, NLRP3, caspase-1, caspase-8, and pro–IL-1β within the same macromolecular complex. These results suggest that the inflammasome is a highly dynamic macromolecular protein complex capable of recruiting different NLRs and effectors to coordinate inflammasome responses to infection. Pathogen recognition by nucleotide-binding oligomerization domain-like receptor (NLR) results in the formation of a macromolecular protein complex (inflammasome) that drives protective inflammatory responses in the host. It is thought that the number of inflammasome complexes forming in a cell is determined by the number of NLRs being activated, with each NLR initiating its own inflammasome assembly independent of one another; however, we show here that the important foodborne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) simultaneously activates at least two NLRs, whereas only a single inflammasome complex is formed in a macrophage. Both nucleotide-binding domain and leucine-rich repeat caspase recruitment domain 4 and nucleotide-binding domain and leucine-rich repeat pyrin domain 3 are simultaneously present in the same inflammasome, where both NLRs are required to drive IL-1β processing within the Salmonella-infected cell and to regulate the bacterial burden in mice. Superresolution imaging of Salmonella-infected macrophages revealed a macromolecular complex with an outer ring of apoptosis-associated speck-like protein containing a caspase activation and recruitment domain and an inner ring of NLRs, with active caspase effectors containing the pro–IL-1β substrate localized internal to the ring structure. Our data reveal the spatial localization of different components of the inflammasome and how different members of the NLR family cooperate to drive robust IL-1β processing during Salmonella infection.
Use of Andexanet Alfa for Factor Xa Inhibitor Reversal in US Verified Trauma Centers: A National Survey

Direct oral factor Xa inhibitors are replacing vitamin K-dependent antagonists as anticoagulation treatment in many clinical scenarios. Trauma centers are noting an increase in patients presenting on these medications. The 2018 Food and Drug Administration approval of andexanet alfa provides an alternative anticoagulation reversal. Barriers may limit utilization of new medications including a lack of grade 1A evidence supporting the use of prothrombin complex concentrate (PCC) versus andexanet alfa and cost. To evaluate barriers of andexanet alfa utilization by trauma surgeons, a 15-question survey was conducted. There was a 9% completion rate (n = 89). The results revealed 23.5% would choose andexanet alfa as first-line treatment in children, and 25.8% as first-line treatment in adults. The majority of respondents, 64.7% and 67.4%, would use PCC preferentially in children and adults, respectively. Respondents indicated that cost burden was an overriding factor (76.3%); 42.4% cited lack of high-level efficacy data of andexanet alfa for reversal of factor Xa inhibitors. Additional double-blinded multi-institutional randomized controlled trials comparing 4F-PCC and andexanet alfa for factor Xa inhibitor reversal are needed to support efficacy especially with the increased cost associated.
Convergence Analyses on On-Line Weight Noise Injection-Based Training Algorithms for MLPs

Injecting weight noise during training is a simple technique that has been proposed for almost two decades. However, little is known about its convergence behavior. This paper studies the convergence of two weight noise injection-based training algorithms, multiplicative weight noise injection with weight decay and additive weight noise injection with weight decay. We consider that they are applied to multilayer perceptrons either with linear or sigmoid output nodes. Let <b>w</b>(<i>t</i>) be the weight vector, let <i>V</i>(<b>w</b>) be the corresponding objective function of the training algorithm, let α >; 0 be the weight decay constant, and let μ(<i>t</i>) be the step size. We show that if μ(<i>t</i>)→ 0, then with probability one <i>E</i>[||<b>w</b>(<i>t</i>)||<sub>2</sub><sup>2</sup>] is bound and lim<i>t</i>→∞||<b>w</b>(<i>t</i>)||<sub>2</sub> exists. Based on these two properties, we show that if μ(<i>t</i>)→ 0, Σ<i>t</i>μ(<i>t</i>)=∞, and Σ<i>t</i>μ(<i>t</i>)<sup>2</sup> <; ∞, then with probability one these algorithms converge. Moreover, <b>w</b>(<i>t</i>) converges with probability one to a point where ∇<b>w</b><i>V</i>(<b>w</b>)=<b>0</b>.
Event-triggered control for multi-agent systems

Event-driven strategies for multi-agent systems are motivated by the future use of embedded microprocessors with limited resources that will gather information and actuate the individual agent controller updates. The control actuation updates considered in this paper are event-driven, depending on the ratio of a certain measurement error with respect to the norm of a function of the state, and are applied to a first order agreement problem. A centralized formulation of the problem is considered first and then the results are extended to the decentralized counterpart, in which agents require knowledge only of the states of their neighbors for the controller implementation.
Closed Reduction and Percutaneous Pinning Versus Open Reduction and Internal Fixation for Type II Lateral Condyle Humerus Fractures in Children Displaced >2 mm

Background: The optimal treatment for pediatric lateral condyle fractures displaced >2 mm, but with little joint displacement is unclear. The purpose of this study was to assess clinical and radiographic outcomes and complication rates of patients undergoing open reduction internal fixation (ORIF) versus closed reduction and percutaneous pin fixation (CRPP) of lateral condyle fractures with >2 mm of displacement and no obvious articular surface incongruity. Methods: A retrospective review of all children with acute lateral condyle fractures with 2.1 to 5.0 mm of displacement treated from 2006 to 2014 was performed. A total of 74 patients were treated who met inclusion criteria; 51 underwent ORIF and 23 underwent CRPP. No differences existed between the 2 groups with respect to age, sex, extremity, mechanism of injury, time to treatment, fracture displacement, or fracture classification. Charts and radiographs were reviewed and the following parameters were documented: operating room time, time to union, return to activities, magnitude of lateral spurring, and complications. Major complications were defined as those with presumptive long-term effects or requiring reoperation. Results: All fractures healed within 12 weeks of surgery, regardless of treatment type and no differences were observed in time to union between groups. OR time averaged 30 minutes faster for the CRPP group (P<0.001). Nearly 10% of patients in each group developed elbow stiffness, requiring formal therapy. The overall complication rates were 25% for the ORIF group and 13% for the CRPP group (P=0.36). No major complications were observed in the CRPP group, whereas 3 (6%) were observed in the ORIF group, including 1 case of avascular necrosis, 1 case of osteomyelitis with an associated premature physeal closure, and 1 refracture requiring surgery. Conclusions: Surgical treatment of lateral condyle fractures displaced >2 mm, but with no significant articular surface incongruity has good outcomes with both CRPP and ORIF. CRPP, however, minimizes surgical time, avoids an incision and is thus our preferred treatment approach when joint congruity can be confirmed. Level of Evidence: Level III—therapeutic study.
Species Differences in Reactivity of Mouse and Rat Astrocytes in vitro

Reactive astrogliosis constitutes a major obstacle to neuronal regeneration and is characterized by rearrangement and upregulation of expression of cytoskeletal proteins, increased proliferation and hypertrophy. Many approaches have been attempted to mimic astrogliosis by inducing reactive astrocytes in vitro. Such research is usually performed using astrocytes derived from Mus musculus or Rattus norvegicus, and results compared between species on the assumption that these cells behave equivalently. Therefore, we compared reactivity between mouse and rat astrocytes in scratch wound assays to gain further insight into how comparable these cell culture models are. Proliferation and migration, as well as expression of the cytoskeletal proteins glial fibrillary acidic protein (GFAP) and vimentin, were compared by immunocytochemistry and immunoblot. Further, we investigated migration of proliferating cells by 5-ethynyl-2′-deoxyuridine staining. Substantial differences in GFAP expression and proliferation between astrocytes of the two species were found: rat astrocytes showed different cytoskeletal morphology, expressed significantly more GFAP and vimentin of different molecular size and were more proliferative than comparable mouse astrocytes. Our results suggest that rat and mouse astrocytes may respond differently to various reactivity-triggering stimuli, which needs to be considered when general conclusions are drawn regarding effects of factors regulating astrocyte reactivity.
RVDT: a design space for multiple input devices, multipleviews and multiple display surfaces combination

We study interaction combination performed using a tabletop device, a mouse, and/or a six Degrees Of Freedom(DOF) input device in a systemcombining a 2Dflat (map-kind) view presented horizontally and a 3D perspective vertical view of the same virtual environment. The design of such a 2D/3D interface relies on the RVDT model and its design space that allow easy high-level combined interactions to achieve spatial tasks. RVDT integrates the relations between physical and numerical DOFs and applies to any graphical user interface in which multiple views, multiple display surfaces and multiple input devices are combined. The user study shows that experimented users prefer table-top/6DOF input device interaction combination with a maximal number of elementary tasks performed with both devices.
Efficiently training a better visual detector with sparse eigenvectors

Face detection plays an important role in many vision applications. Since Viola and Jones proposed the first real-time AdaBoost based object detection system, much effort has been spent on improving the boosting method. In this work, we first show that feature selection methods other than boosting can also be used for training an efficient object detector. In particular, we have adopted greedy sparse linear discriminant analysis (GSLDA) for its computational efficiency; and slightly better detection performance is achieved compared with. Moreover, we propose a new technique, termed boosted greedy sparse linear discriminant analysis (BGSLDA), to efficiently train object detectors. BGSLDA exploits the sample re-weighting property of boosting and the class-separability criterion of GSLDA. Experiments in the domain of highly skewed data distributions, e.g., face detection, demonstrates that classifiers trained with the proposed BGSLDA outperforms AdaBoost and its variants. This finding provides a significant opportunity to argue that Adaboost and similar approaches are not the only methods that can achieve high classification results for high dimensional data such as object detection.
Stress, self‐efficacy and quality of life in cancer patients

The purpose of the study was to examine the effect of different stressors on various domains of quality of life (QOL) in cancer patients. The study focused on testing a model describing interrelations between two kinds of stress antecedents, two mediating variables—perceived stress and self‐efficacy—and QOL. The participants were 60 cancer patients of both genders and various diagnoses. They were administered questionnaires of background information, QOL, perceived stress and general self‐efficacy. Two stress indices were defined empirically: health stress (based on advanced disease stage, long disease duration, and undergoing treatment) and social stress (based on unemployment, recent immigration, and older age). Confirmatory factor analysis enabled defining five factors of QOL. Hierarchical regression analyses showed that the index of social stress was related to more QOL scales than the index of health stress and very few interactions with the mediating variables. Structural equation modeling provided a more comprehensive and accurate view. It showed that the index of health stress affected QOL mainly through perceived stress, and that self‐efficacy affected QOL by reducing perceived stress and increasing QOL. The major conclusions are that QOL is affected negatively by both health stresses and social stresses, but the former are mediated primarily by the experience of perceived stress. Copyright © 2006 John Wiley & Sons, Ltd.
Differential binding properties of [H-3]dextrorphan and [H-3]MK-801 in heterologously expressed NMDA receptors

The N -methyl-D-aspartate receptor (NMDAR) antagonists: MK-801, phencyclidine and ketamine are open-channel blockers with limited clinical value due to psychotomimetic effects. Similarly, the psychotomimetic effects of the dextrorotatory opioids, dextromethorphan and its metabolite dextrorphan, derive from their NMDAR antagonist actions. Differences in the use dependency of blockade, however, suggest that the binding sites for MK-801 and dextrorphan are distinct. In the absence of exogenous glutamate and glycine, the rate of association of [ 3 H]MK-801 with wild-type NR1-1a/NR2A receptors was considerably slower than that for [ 3 H]dextrorphan. Glutamate individually, and in the presence of the co-agonist glycine, had substantial effects on the specific binding of [ 3 H]MK-801, while the binding of [ 3 H]dextrorphan was not affected. Mutation of residues N616 and A627 in the NR1 subunit had a profound effect on [ 3 H]MK-801 binding affinity, while that of [ 3 H]dextrorphan was unaltered. In contrast, NR1 residues, W611 and N812, were critical for specific binding of [ 3 H]dextrorphan to NR1-1a/NR2A complexes with no corresponding influence on that of [ 3 H]MK-801. Thus, [ 3 H]dextrorphan and [ 3 H]MK-801 have distinct molecular determinants for high-affinity binding. The ability of [ 3 H]dextrorphan to bind to a closed channel, moreover, indicates that its recognition site is shallower in the ion channel domain than that of MK-801 and may be associated with the extracellular vestibule of the NMDAR.
Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan

A multidisciplinary, collaborative project was conducted to determine the prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan and ascertain its Public Health Significance. Using a grid-based sampling strategy, soil samples (n = 145) were collected from villages (n = 29, 5 samples/village) and examined for Bacillus anthracis, Burkholderia mallei/pseudomallei, Coxiella burnetii, Francisella tularensis, and Yersinia pestis using real time PCR assays. Chemical analysis of soil samples was also performed on these samples. The relationship between soil composition and absence or presence of the pathogen, and seven risk factors was evaluated. DNA of B. anthracis (CapB), B. mallei/pseudomallei (chromosomal gene), C. burnetii (IS1111, transposase gene), and F. tularensis (lipoprotein/outer membrane protein) was detected in 9.6, 1.4, 4.8, and 13.1% of soil samples, respectively. None of the samples were positive for protective antigen plasmid (PA) of B. anthracis and Y. pestis (plasminogen activating factor, pPla gene). The prevalence of B. anthracis (CapB) was found to be associated with organic matter, magnesium (Mg), copper (Cu), chromium (Cr), manganese (Mn), cobalt (Co), cadmium (Cd), sodium (Na), ferrous (Fe), calcium (Ca), and potassium (K). Phosphorous (P) was found to be associated with prevalence of F. tularensis while it were Mg, Co, Na, Fe, Ca, and K for C. burnetii. The odds of detecting DNA of F. tularensis were 2.7, 4.1, and 2.7 higher when soil sample sites were >1 km from animal markets, >500 m from vehicular traffic roads and animal density of < 1000 animals, respectively. While the odds of detecting DNA of C. burnetii was 32, 11.8, and 5.9 higher when soil sample sites were >500 m from vehicular traffic roads, presence of ground cover and animal density of < 1000 animals, respectively. In conclusion, the distribution pattern of the soil-borne pathogens in and around the areas of Lahore district puts both human and animal populations at a high risk of exposure. Further studies are needed to explore the genetic nature and molecular diversity of prevailing pathogens together with their seroconversion in animals and humans.
Deep scattering spectra with deep neural networks for LVCSR tasks

Log-mel filterbank features, which are commonly used features for CNNs, can remove higher-resolution information from the speech signal. A novel technique, known as Deep Scattering Spectrum (DSS), addresses this issue and looks to preserve this information. DSS features have shown promise on TIMIT, both for classification and recognition. In this paper, we extend the use of DSS features for LVCSR tasks. First, we explore the optimal multi-resolution time and frequency scattering operations for LVCSR tasks. Next, we explore techniques to reduce the dimension of the DSS features. We also incorporate speaker adaptation techniques into the DSS features. Results on a 50 and 430 hour English Broadcast News task show that the DSS features provide between a 4-7% relative improvement in WER over log-mel features, within a state-of-the-art CNN framework which incorporates speaker-adaptation and sequence training. Finally, we show that DSS features are similar to multi-resolution log-mel + MFCCs, and similar improvements can be obtained with this representation.
Seismic signature of active intrusions in mountain chains

Magma pulses in mountain chains may trigger earthquakes relevant to seismic hazard assessment. Intrusions are a ubiquitous component of mountain chains and testify to the emplacement of magma at depth. Understanding the emplacement and growth mechanisms of intrusions, such as diapiric or dike-like ascent, is critical to constrain the evolution and structure of the crust. Petrological and geological data allow us to reconstruct magma pathways and long-term magma differentiation and assembly processes. However, our ability to detect and reconstruct the short-term dynamics related to active intrusive episodes in mountain chains is embryonic, lacking recognized geophysical signals. We analyze an anomalously deep seismic sequence (maximum magnitude 5) characterized by low-frequency bursts of earthquakes that occurred in 2013 in the Apennine chain in Italy. We provide seismic evidences of fluid involvement in the earthquake nucleation process and identify a thermal anomaly in aquifers where CO2 of magmatic origin dissolves. We show that the intrusion of dike-like bodies in mountain chains may trigger earthquakes with magnitudes that may be relevant to seismic hazard assessment. These findings provide a new perspective on the emplacement mechanisms of intrusive bodies and the interpretation of the seismicity in mountain chains.
Elevated [Cl-]i, and [Na+]i inhibit Na+, K+, Cl- cotransport by different mechanisms in squid giant axons

Bumetanide-sensitive (BS) unidirectional fluxes of (36)Cl- or (22)Na+ were measured in internally dialyzed squid giant axons while varying the intra- or extracellular concentrations of Na+ and/or Cl-. Raising either [Cl-]i or [Na+]i resulted in a concentration-dependent reduction of the BS influx of both (36)Cl- and (22)Na+. Raising [Cl-]i above 200 mM completely blocked BS influxes. However, raising [Na+]i to 290 mM resulted in saturable but incomplete inhibition of both BS Na+ influx and BS Cl- influx. The consequences of varying intracellular Cl- on cotransporter effluxes were complex. At lower [Cl-]i values (below 100 mM) intracellular Cl- activated cotransporter effluxes. Surprisingly, however, raising [Cl-]i levels > 125 mM resulted in a [Cl-]i-dependent inhibition of BS effluxes of both Na+ and Cl-. On the other hand, raising [Na+]i resulted only in the activation of the BS Na+ efflux; intracellular Na+ did not inhibit BS efflux even at 290 mM. The inhibitory effects of intracellular Na+ on cotransporter-mediated influxes, and lack of inhibitory effects on BS effluxes, are consistent with the trans-side inhibition expected for an ordered binding/release model of cotransporter operation. However, the inhibitory effects of intracellular Cl- on both influxes and effluxes are not explained by such a model. These data suggest that Cl may interact with an intracellular site (or sites), which does not mediate Cl transport, but does modulate the transport activity of the Na+, K+, Cl- cotransporter.