Miracle-dz/UniFAR-FTUs huggingface dataset:
A Design to Study Flow Induced Crystallization in a Multipass Rheometer

Abstract The design and performance of a flow geometry for the multipass rheometer (MPR) is described, creating an experimental setup to study in-situ and ex-situ structure and morphology development with a proper control over the processing conditions and shear history. The slit used is equipped with diamond windows, to combine flow with different experimental techniques, such as optical microscopy (OM), birefringence and X-ray scattering. In this paper we present preliminary results, obtained on isotactic polypropylene, that demonstrate the possibilities of this device for more extended future research. The focus is on the in-situ birefringence measurements of crystallization and the relation with the final morphology.
IL-10 Gene Transfer Attenuates P. gingivalis-induced Inflammation

IL-10 is an anti-inflammatory cytokine secreted by stimulated Th2 lymphocytes that can down-regulate inflammatory responses to bacterial challenge. We hypothesized that local delivery of IL-10 using gene-transfer will down-regulate inflammatory responses. We examined the effect of IL-10 plasmid injection on the local cytokine response. Two weeks after the implantation of chambers, either IL-10 plasmid or vector was injected into the mice. Four days later, they were challenged with an intra-chamber injection of P. gingivalis. The intra-chamber levels of IL-10, IFNγ, TNFα, and IL-1β were evaluated after 2 and 24 hrs. The results showed that local IL-10 gene delivery elevated the levels of IL-10 at both time periods. It attenuated the levels of IFNγ (656 ± 154 to 218 ± 144 pg/mL) and TNFα (23 ± 2.0 to 12.5 ± 2.9 ng/mL) at 2 hrs, and of IL-1β (21.5 ± 5.7 to 12.4 ± 3.0 ng/mL) at 24 hrs. The results suggest the possibility of modulating the local inflammatory response to P. gingivalis by direct IL-10 gene transfer.
Study on the Gemological Characteristics of Amber from Myanmar and Chinese Fushun

This paper researches on the gemological characteristics of amber from Myanmar and Chinese Fushun by using of the basic instruments of gemology, SEM and FT-IR. The results show that the refractive index is approximately 1.54 both in Myanmar and Fushun amber, resin glossy as well as conchoidal fracture. While the hardness of Myanmar amber is much higher and it has a dark lyons blue ultraviolet fluorescence; Fushun amber is much more transparent, medium kashima ultraviolet fluorescence and coal coveres on its surface. In addition, the fracture in Myanmar amber is more obvious compared with Fushun amber.The infrared spectrums prove that Myanmar amber has the absorption peak of CO stretching vibration at 1730cm-1, while Fushun amber is at 1700cm; the absorption peak of CH3 asymmetric stretching at 2950cm-1 of Myanmar amber is also different from Fushun amber which is at 2960cm-1. As a result, this paper provides basic identification between Myanmar amber and Chinese Fushun amber in gemology.
Parameter estimation of neuron models using in-vitro and in-vivo electrophysiological data

Spiking neuron models can accurately predict the response of neurons to somatically injected currents if the model parameters are carefully tuned. Predicting the response of in-vivo neurons responding to natural stimuli presents a far more challenging modeling problem. In this study, an algorithm is presented for parameter estimation of spiking neuron models. The algorithm is a hybrid evolutionary algorithm which uses a spike train metric as a fitness function. We apply this to parameter discovery in modeling two experimental data sets with spiking neurons; in-vitro current injection responses from a regular spiking pyramidal neuron are modeled using spiking neurons and in-vivo extracellular auditory data is modeled using a two stage model consisting of a stimulus filter and spiking neuron model.
Predicting Gene Expression from Sequence: A Reexamination

Although much of the information regarding genes' expressions is encoded in the genome, deciphering such information has been very challenging. We reexamined Beer and Tavazoie's (BT) approach to predict mRNA expression patterns of 2,587 genes in Saccharomyces cerevisiae from the information in their respective promoter sequences. Instead of fitting complex Bayesian network models, we trained naïve Bayes classifiers using only the sequence-motif matching scores provided by BT. Our simple models correctly predict expression patterns for 79% of the genes, based on the same criterion and the same cross-validation (CV) procedure as BT, which compares favorably to the 73% accuracy of BT. The fact that our approach did not use position and orientation information of the predicted binding sites but achieved a higher prediction accuracy, motivated us to investigate a few biological predictions made by BT. We found that some of their predictions, especially those related to motif orientations and positions, are at best circumstantial. For example, the combinatorial rules suggested by BT for the PAC and RRPE motifs are not unique to the cluster of genes from which the predictive model was inferred, and there are simpler rules that are statistically more significant than BT's ones. We also show that CV procedure used by BT to estimate their method's prediction accuracy is inappropriate and may have overestimated the prediction accuracy by about 10%.
Up-regulation of miR-210 by vascular endothelial growth factor in ex vivo expanded CD34+ cells enhances cell-mediated angiogenesis

Ex vivo culture has been proposed as a means to augment and repair autologous cells in patients with chronic diseases, but the mechanisms governing improvement in cell function are not well understood. Although microRNAs (miRs) are increasingly appreciated as key regulators of cellular function, a role for these factors in CD34+ cell‐mediated angiogenesis has not been elucidated. Vascular endothelial growth factor (VEGF) was previously shown to induce expression of certain miRs associated with angiogenesis in endothelial cells and promote survival and number of vascular colony forming units of haematopoietic stem cells (HSCs). We sought to evaluate the role of VEGF in expansion and angiogenic function of CD34+ cells and to identify specific miRs associated with angiogenic properties of expanded cells. Umbilical cord blood CD34+ cells were effectively expanded (18‐ to 22‐fold) in culture medium containing stem cell factor (SCF), Flt‐3 ligand (Flt‐3), thrombopoietin (TPO) and interleukin‐6 (IL‐6) with (postEX/+VEGF) and without VEGF (postEX/noVEGF). Tube formation in matrigel assay and tissue perfusion/capillary density in mice ischaemic hindlimb were significantly improved by postEX/+VEGF cells compared with fresh CD34+ and postEX/noVEGF cells. MiR‐210 expression was significantly up‐regulated in postEX/+VEGF cells. MiR‐210 inhibitor abrogated and 210 mimic recapitulated the pro‐angiogenic effects by treatment of postEX/+VEGF and postEX/noVEGF cells respectively. Collectively, these observations highlight a critical role for VEGF in enhancing the angiogenic property of expanded cells, and identify miR‐210 as a potential therapeutic target to enhance CD34+ stem cell function for the treatment of ischaemic vascular disease.
The human urothelial tight junction: claudin 3 and the ZO-1α+ switch

Objective Tight junctions are multicomponent structures, with claudin proteins defining paracellular permeability. Claudin 3 is a candidate for the exceptional “tightness” of human urothelium, being localised to the terminal tight junction (TJ) of superficial cells. Our aim was to determine whether claudin 3 plays an instigating and/or a functional role in the urothelial TJ. Materials and Methods Normal human urothelial (NHU) cells maintained as non-immortalised cell lines were retrovirally-transduced to over-express or silence claudin 3 expression. Stable sublines induced to stratify or differentiate were assessed for TJ formation by immunocytochemistry and transepithelial electrical resistance (TER). Expression of claudin 3, ZO-1 and ZO-1α+ was examined in native urothelium by immunohistochemistry. Results Claudin 3 expression was associated with differentiation and development of a tight barrier and along with ZO-1 and ZO-1α+ was localised to the apical tight junction in native urothelium. Knockdown of claudin 3 inhibited formation of a tight barrier in three independent cell lines, however, overexpression of claudin 3 was not sufficient to induce tight barrier development in the absence of differentiation. A differentiation-dependent induction of the ZO-1α+ isoform was found to coincide with barrier formation. Whereas claudin 3 overexpression did not induce the switch to co-expression of ZO-1α−/ZO-1α+, claudin 3 knockdown decreased localisation of ZO-1 to the TJ and resulted in compromised barrier function. Conclusions Urothelial cytodifferentiation is accompanied by induction of claudin 3 which is essential for the development of a terminal TJ. A coordinated switch to the ZO-1α+ isotype was also observed and for the first time may indicate that ZO-1α+ is involved in the structural assembly and function of the urothelial terminal TJ.
Hybrid Control of the Berkeley Lower Extremity Exoskeleton (BLEEX)

The Berkeley Lower Extremity Exoskeleton is the first functional energetically autonomous load carrying human exoskeleton and was demonstrated at U.C. Berkeley, walking at the average speed of 0.9 m/s (2 mph) while carrying a 34 kg (75 lb) payload. The original published controller, called the BLEEX Sensitivity Amplification Controller, was based on positive feedback and was designed to increase the closed loop system sensitivity to its wearer’s forces and torques without any direct measurement from the wearer. This controller was successful at allowing natural and unobstructed load support for the pilot. This article presents an improved control scheme we call “hybrid” BLEEX control that adds robustness to changing BLEEX backpack payload. The walking gait cycle is divided into stance control and swing control phases. Position control is used for the BLEEX stance leg (including the torso and backpack) and a sensitivity amplification controller is used for the swing leg. The controller is also designed to smoothly transition between these two schemes as the pilot walks. With hybrid control, the controller does not require a good model of the BLEEX torso and payload, which is difficult to obtain and subject to change as payload is added and removed. As a tradeoff, the position control used in this method requires the human to wear seven inclinometers to measure human limb and torso angles. These additional sensors require careful design to securely fasten them to the human and increase the time to don and doff BLEEX.
Enhanced transdermal delivery of salbutamol sulfate via ethosomes

The main objective of the present work was to compare the transdermal delivery of salbutamol sulfate (SS), a hydrophilic drug used as a bronchodilator, from ethosomes and classic liposomes containing different cholesterol and dicetylphosphate concentrations. All the systems were characterized for shape, particle size, and entrapment efficiency percentage, by image analysis optical microscopy or transmission electron microscopy, laser diffraction, and ultracentrifugation, respectively. In vitro drug permeation via a synthetic semipermeable membrane or skin from newborn mice was studied in Franz diffusion cells. The selected systems were incorporated into Pluronic F 127 gels and evaluated for both drug permeation and mice skin deposition. In all systems, the presence of spherical-shaped vesicles was predominant. The vesicle size was significantly decreased (P<.05) by decreasing cholesterol concentration and increasing dicetylphosphate and ethanol concentrations. The entrapment efficiency percentage was significantly increased (P<.05) by increasing cholesterol, dicetylphosphate, and ethanol concentrations. In vitro permeation studies of the prepared gels containing the selected vesicles showed that ethosomal systems were much more efficient at delivering SS into mice skin (in terms of quantity and depth) than were liposomes or aqueous or hydroalcoholic solutions.
Global decline of bumblebees is phylogenetically structured and inversely related to species range size and pathogen incidence

Conservation biology can profit greatly from incorporating a phylogenetic perspective into analyses of patterns and drivers of species extinction risk. We applied such an approach to analyse patterns of bumblebee (Bombus) decline. We assembled a database representing approximately 43% of the circa 260 globally known species, which included species extinction risk assessments following the International Union fo Conservation of Nature Red List categories and criteria, and information on species traits presumably associated with bumblebee decline. We quantified the strength of phylogenetic signal in decline, range size, tongue length and parasite presence. Overall, about one-third of the assessed bumblebees are declining and declining species are not randomly distributed across the Bombus phylogeny. Susceptible species were over-represented in the subgenus Thoracobombus (approx. 64%) and under-represented in the subgenus Pyrobombus (approx. 6%). Phylogenetic logistic regressions revealed that species with small geographical ranges and those in which none of three internal parasites were reported (i.e. Crithidia bombi, Nosema spp. or Locustacarus buchneri) were particularly vulnerable. Bumblebee evolutionary history will be deeply eroded if most species from threatened clades, particularly those stemming from basal nodes, become finally extinct. The habitat of species with restricted distribution should be protected and the importance of pathogen tolerance/resistance as mechanisms to deal with pathogens needs urgent research.
Demographic and Genetic Estimates of Effective Population and Breeding Size in the Amphibian Rana temporaria

Abstract: Genetic methods for estimating effective population size ( Ne) or the effective number of breeders ( Nb) have become popular, but comparisons of these estimates with demographic estimates of Neand Nbare rare, especially in anurans. We used three genetic (linkage disequilibrium, temporal moments, Bayesian coalescent‐based method) and three demographic models, the latter considering number of breeding individuals, sex ratio, reproductive skew, and other demographic data, to estimate Neand Nbin two subarctic populations (T and P) of the common frog Rana temporaria, subject to long‐term capture–recapture studies. Demographic estimates of Nebased on total population size ( Ne[T]= 44.5–56.9; Ne[P]= 68.8–93.7) deviated markedly from the genetic estimates obtained using the linkage disequilibrium method ( Ne[T]= 97.1; Ne[P]= 13.2). The demographic estimates of Nb, taking into consideration sex ratio and variance in reproductive success ( Nb[T]= 10.1–39.7; Nb[P]= 3.9–21.3), were higher than the genetic estimates ( Nb[T]= 3.7–5.4; Nb[P]= 3.5–3.9). The main factors affecting the effective size estimates were sex ratio and reproductive skew. The discrepancies between corresponding Neand Nbestimates highlight the sensitivity of both demographic and genetic estimates on their underlying assumptions. Yet the ratios of effective or breeding effective size to the census population size were similar to those reported earlier for anurans, reinforcing the view that the discrepancy between actual and effective breeding sizes in anuran populations is typically very large.
Joint damage and inflammation in c-Jun N-terminal kinase 2 knockout mice with passive murine collagen-induced arthritis.

OBJECTIVE Previous studies have demonstrated that inhibition of c-Jun N-terminal kinase (JNK) decreases joint destruction in the rat adjuvant arthritis model. The present study was undertaken to investigate whether selective loss of JNK-2 function decreases joint destruction in JNK-2 knockout mice, in order to determine the role of this isoform in inflammatory arthritis. METHODS Passive collagen-induced arthritis (CIA) was induced in Jnk2(-/-) and wild-type mice by administering anti-type II collagen antibodies. Arthritis was assessed daily using a semiquantitative clinical scoring system. Fibroblast-like synoviocytes (FLS) were prepared from Jnk2(-/-) and wild-type mice, and JNK protein expression was determined by Western blot analysis. Matrix metalloproteinase 13 (MMP-13) expression was determined by Northern blot analysis, and activator protein 1 (AP-1) binding activity by electromobility shift assay (EMSA). RESULTS The JNK protein level in Jnk2(-/-) mice with CIA was 22% of that in wild-type mice with CIA (P < 0.001), and mainly the 46-kd isoform was expressed in the former group. Surprisingly, clinical arthritis was slightly more severe in the Jnk2(-/-) mice. Histologic scores for synovial inflammation were not significantly different. However, Safranin O-stained sections from the Jnk2(-/-) mice exhibited significantly less joint damage. Although joint destruction was decreased in Jnk2(-/-) mice with CIA, EMSA and Northern blot analysis of total joint extracts revealed similar levels of AP-1 binding and MMP-13 expression in Jnk2(-/-) and wild-type mice. The lack of correlation with AP-1 activity and MMP expression was probably because non-FLS cells in the joint may express more JNK-1 than do FLS. CONCLUSION JNK-2 is a determinant of matrix degradation, but it has little effect on inflammation in arthritis. Complete inhibition of MMP expression and joint destruction will likely require combined JNK-1 and JNK-2 inhibition.
Incidence, character and clinical relevance of mixed cryoglobulinaemia in patients with chronic hepatitis C virus infection

Hepatitis C virus (HCV) infection has been implicated in the pathogenesis of mixed cryoglobulinaemia. Several studies have shown the presence of anti‐HCV antibodies and HCV‐RNA in both sera and cryoglobulins of such patients. However, the prevalence and clinical significance of cryoglobulins remain uncertain in patients with chronic HCV infection. We have studied 113 consecutive patients referred for assessment because of the presence of anti‐HCV antibody in serum for the presence of cryoglobulinaemia and ascertained their clinical relevance and immunochemical properties. Twenty‐one of 113 (19%) had detectable cryoglobulins with a mean protein concentration of 0.38 g/l (range 0.15–3.34 g/l). Most of these patients were asymptomatic. The cryoglobulins were of type III in 19 (91%) and of type II in two patients (9%). The latter two patients had the highest concentration of cryoglobulins, subnormal C4 and C1q levels suggesting classical pathway activation and vasculitis with renal impairment. The cryoglobulin IgG subclasses were mainly IgG1 and IgG3. HCV‐RNA was detected more frequently in the sera of cryoglobulin‐positive patients than in cryoglobulin‐negative patients. This study showed that mixed cryoglobulinaemia is common in chronic HCV infection, and is predominantly type III. Evidence of systemic or renal disease was rare except in those with type II cryoglobulinaemia, and this may reflect either the concentration of the cryoprecipitate or the presence of a monoclonal complement‐activating IgM paraprotein. The detection of HCV‐RNA in the majority of the cryoprecipitates further supports the important role of HCV in the etiopathogenesis of essential mixed cryoglobulinaemia, although the mechanism is at present unclear.
Exposure to tobacco smoking and periodontal health.

BACKGROUND The influence of smoking behavior on the periodontal health condition was clinically and radiographically studied in 257 dentally aware adults in the age range 20-69 years, including 50 current smokers, 61 former smokers and 133 non-smokers. AIMS The clinical variables to be investigated were frequency of diseased sites > or =4 mm, frequency of gingival bleeding sites and plaque index. In addition, the periodontal bone height was radiographically assessed as a % of the dental root length. METHODS All variables were based on full-mouth examinations including all teeth and periodontia. RESULTS The observations indicated an inferior periodontal health condition associated with smoking. This was evidenced by a significantly greater frequency of diseased sites and a significantly greater reduction of periodontal bone height in current smokers as compared to non-smokers. The condition of former smokers was intermediate between current smokers and non-smokers, suggesting that former smokers who have quit smoking have a better periodontal health condition than current smokers, although worse than that of non-smokers. The finding that former smokers exhibited less disease than current smokers suggests that smoking cessation may be beneficial and mitigate the untoward effects inflicted by smoking, allowing a normalization towards non-smoker conditions. Heavy exposure was consistently associated with more severe a condition than light exposure, suggesting that the relationship between smoking exposure and periodontal morbidity is dose-dependent. CONCLUSIONS Altogether, the present observations identify a negative impact from smoking on periodontal health and provide further evidence that tobacco smoking is an avoidable risk for periodontal disease.
Physical Activity and Mortality in Older Men With Diagnosed Coronary Heart Disease

BackgroundWe have studied the relations between physical activity, types of physical activity, and changes in physical activity and all-cause mortality in men with established coronary heart disease (CHD). Methods and ResultsIn 1992, 12 to 14 years after the initial screening (Q1) of 7735 men 40 to 59 years of age from general practices in 24 British towns, 5934 (91% of available survivors, mean age 63 years) provided further information on physical activity (Q92) and were followed up for 5 years; 963 had a physician’s diagnosis of CHD (myocardial infarction or angina). After exclusions, there were 772 men with established CHD, 131 of whom died of all causes. The lowest risks for all-cause and cardiovascular mortality were seen in light and moderate activity groups (adjusted relative risk compared with inactive/occasionally active: light, 0.42 (0.25, 0.71); moderate, 0.47 (0.24, 0.92); and moderately vigorous/vigorous, 0.63 (0.39, 1.03). Recreational activity of ≥4 hours per weekend, moderate or heavy gardening, and regular walking (>40 min/d) were all associated with a significant reduction in all-cause mortality. Nonsporting activity was more beneficial than sporting activities. Men sedentary at Q1 who began at least light activity by Q92 showed lower mortality rates on follow-up than those who remained sedentary ( relative risk 0.58, 95% CI 0.33 to 1.03;P =0.06). ConclusionsLight or moderate activity in men with established CHD is associated with a significantly lower risk of all-cause mortality. Regular walking and moderate or heavy gardening were sufficient to achieve this benefit.