Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact of electromagnetic fields on stem cells: common mechanisms at the crossroad between adult neurogenesis and osteogenesis

In the recent years adult neural and mesenchymal stem cells have been intensively investigated as effective resources for repair therapies. In vivo and in vitro studies have provided insights on the molecular mechanisms underlying the neurogenic and osteogenic processes in adulthood. This knowledge appears fundamental for the development of targeted strategies to manipulate stem cells. Here we review recent literature dealing with the effects of electromagnetic fields on stem cell biology that lends support to their use as a promising tool to positively influence the different steps of neurogenic and osteogenic processes. We will focus on recent studies revealing that extremely-low frequency electromagnetic fields enhance adult hippocampal neurogenesis by inducing epigenetic modifications on the regulatory sequences of genes responsible for neural stem cell proliferation and neuronal differentiation. In light of the emerging critical role played by chromatin modifications in maintaining the stemness as well as in regulating stem cell differentiation, we will also attempt to exploit epigenetic changes that can represent common targets for electromagnetic field effects on neurogenic and osteogenic processes.
Anemia and Acute Coronary Syndrome: Time for Intervention Studies

Originally characterized as “globules of his blood” in 1675 by the Dutch microscopist van Leeuwenhoek, the crucial role of erythrocytes, or red blood cells, in regulating normal human physiology was not appreciated until centuries later. [1][1] In contemporary medical taxonomy, the construct of
Partial Inhibition of Multidrug Resistance by Safingol Is Independent of Modulation of P-glycoprotein Substrate Activities and Correlated with Inhibition of Protein Kinase C (*)

Safingol is a lysosphingolipid protein kinase C (PKC) inhibitor that competitively interacts at the regulatory phorbol binding domain of PKC. We investigated the effects of safingol on antineoplastic drug sensitivity and PKC activity of MCF-7 tumor cell lines. Safingol treatment of 32P-labeled MCF-7 WT and MCF-7 DOXR cells inhibited phosphorylation of the myristoylated alanine-rich protein kinase C substrate in both cell lines, suggesting inhibition of cellular PKC. However, only in MCF-7 DOXR cells did safingol treatment increase accumulation of [3H]vinblastine and enhance toxicity of Vinca alkaloids and anthracyclines. Drug accumulation changes in MCF-7 DOXR cells treated with safingol were accompanied by inhibition of basal and phorbol 12,13-dibutyrate-stimulated phosphorylation of P-glycoprotein (P-gp). Expression of P-gp and levels of mdr1 message in MCF-7 DOXR cells were not altered by safingol treatment alone or in combination with vinblastine. Treatment of MCF-7 DOXR cell membranes with safingol did not inhibit [3H]vinblastine binding or [3H]azidopine photoaffinity labeling of P-gp. Furthermore, safingol did not stimulate P-gp ATPase activity in membranes prepared from MCF-7 DOXR cells. We conclude that enhanced drug accumulation and sensitivity in MCF-7 DOXR cells treated with safingol are correlated with inhibition of PKC rather than competitive interference with P-gp drug binding through direct interaction with P-glycoprotein.
Coupled information-theoretic encoding for face photo-sketch recognition

Automatic face photo-sketch recognition has important applications for law enforcement. Recent research has focused on transforming photos and sketches into the same modality for matching or developing advanced classification algorithms to reduce the modality gap between features extracted from photos and sketches. In this paper, we propose a new inter-modality face recognition approach by reducing the modality gap at the feature extraction stage. A new face descriptor based on coupled information-theoretic encoding is used to capture discriminative local face structures and to effectively match photos and sketches. Guided by maximizing the mutual information between photos and sketches in the quantized feature spaces, the coupled encoding is achieved by the proposed coupled information-theoretic projection tree, which is extended to the randomized forest to further boost the performance. We create the largest face sketch database including sketches of 1, 194 people from the FERET database. Experiments on this large scale dataset show that our approach significantly outperforms the state-of-the-art methods.
Terrain mapping for a roving planetary explorer

The authors are prototyping a legged vehicle, the Ambler, for an exploratory mission on another planet, conceivably Mars, where it is to traverse uncharted areas and collect material samples. They describe how the rover can construct from range imagery a geometric terrain representation, i.e., elevation map that includes uncertainty, unknown areas, and local features. First, they present an algorithm for constructing an elevation map from a single range image. By virtue of working in spherical-polar space, the algorithm is independent of the desired map resolution and the orientation of the sensor, unlike algorithms that work in Cartesian space. Secondly, the authors present a two-stage matching technique (feature matching followed by iconic matching) that identifies the transformation T corresponding to the vehicle displacement between two viewing positions. Thirdly, to support legged locomotion over rough terrain, they describe methods for evaluating regions of the constructed elevation maps as footholds. <<ETX>>
The plus ends of stable microtubules are the exclusive nucleating structures for microtubules in the axon

Microtubules (MTs) in the axon have a uniform polarity orientation that is recapitulated during recovery from episodes of MT depolymerization (Heidemann, S. R., M. A. Hamborg, S. J. Thomas, B. Song, S. Lindley, and D. Chu. 1984. J. Cell Biol. 99:1289-1295). This tight regulation of their organization indicates that axonal MTs are spatially regulated by discrete nucleating structures comparable in function to the centrosome. Several authors have proposed that an especially stable class of MTs in the axon may serve as these nucleating structures. In a previous report (Baas, P. W., and M. M. Black. 1990. J. Cell Biol. 111:495-509), we determined that the axons of cultured sympathetic neurons contain two classes of MT polymer, stable and labile, that differ in their sensitivity to nocodazole by roughly 35-fold. The stable and labile polymer represent long-lived and recently assembled polymer, respectively. We also determined that these two classes of polymer can be visually distinguished at the immunoelectron microscopic level based on their content of tyrosinated alpha-tubulin: the labile polymer stains densely, while the stable polymer does not stain. In the present study, we have taken advantage of these observations to directly identify MT nucleating structures in the axon. Neuron cultures were treated with nocodazole for 6 h to completely depolymerize the labile polymer in the axon, and substantially shorten the stable polymer. The cultures were then rinsed free of the drug, permitted to reassemble polymer for various periods of time, and prepared for immunoelectron microscopic localization of tyrosinated alpha-tubulin. Serial reconstruction of consecutive thin sections was undertaken to determine the spatial relationship between the stable MTs and the newly assembled polymer. All of the new polymer assembled in direct continuity with the plus ends of stable MTs, indicating that these ends are assembly competent, and hence capable of acting as nucleating structures. Our results further indicate that no self-assembly of MTs occurs in the axon, nor do any MT nucleating structures exist in the axon other than the plus ends of stable MTs. Thus the plus ends of stable MTs are the exclusive nucleating structures for MTs in the axon.
On the Performance of Turbo Signal Recovery with Partial DFT Sensing Matrices

This letter is on the performance of the turbo signal recovery (TSR) algorithm for partial discrete Fourier transform (DFT) matrices based compressed sensing. Based on state evolution analysis, we prove that TSR with a partial DFT sensing matrix outperforms the well-known approximate message passing (AMP) algorithm with an independent identically distributed (IID) sensing matrix.
Investigation of the Precipitates on the Concrete Surface due to Sulphate Exposure

Abstract The aim of this study is to investigate the durability of cement-based materials subjected to the effects of sulphuric acid in terms of surface deterioration. Damaged concrete surfaces and the samples’ mass changes were studied during 270-day simulation of both chemical and biological attacks. Chemical corrosion was simulated by sulphuric acid with pH of 3.0 and 4.0, respectively, while biological corrosion was simulated by activity of bacteria Acidithiobacillus thiooxidans. XRD and SEM analyses confirmed a massive sulphate precipitate formation on the concrete surface due to chemical and biological sulphate corrosion.
Variational conditions and conjugate points for the fixed-endpoint control problem

For the fixed-endpoint optimal-control problem, with the set of constraints on the state and control functions arbitrary, we provide in this paper a definition of conjugate point together with a set of sufficient conditions for weak and strong local optimality. These conditions, which are a strengthening of known necessary conditions, involve the second variation with respect to the Hamiltonian, and are obtained directly without any reference to possible extended definitions of conjugate points, fields of extremals, solutions of a certain Riccati equation, or Hamilton-Jaco bi theory. In other words, the proof of sufficiency is self-contained in optimal-control terms. On the other hand, the notion of conjugate point is derived by a characterization of the positivity of the second variation on a set of nontrivial admissible variations, and the definition we give is 'proper' in the sense that the corresponding Jacobi conditions play, for necessity and sufficiency, the same role as in the classical theory of calculus of variations.
The neural system that mediates familiarity memory

In recognition memory tests, feelings of familiarity for stimuli vary in strength. Increasing levels of felt familiarity should modulate activity in brain structures that mediate familiarity memory. We used this expectation to identify the neural system that underlies scene familiarity memory. Normal subjects studied pictures of scenes and 2 days later while undergoing event‐related functional magnetic resonance imaging (fMRI) rated old and new pictures as novel, slightly familiar, moderately familiar, very familiar, or recollected, although they were specifically instructed not to try and recollect. Familiarity strength was, therefore, judged as absent (misses) or present at three levels of increasing strength. A parametric analysis showed that, as perceived strength of familiarity increased activity in the perirhinal cortex, insula and left superior temporal cortex declined linearly whereas activity in the left dorsomedial thalamus, left ventrolateral and anteromedial frontal cortex, posterior cingulate cortex, and left parietal neocortex increased linearly. Hippocampal activity was not modulated linearly or quadratically by changes in familiarity strength. Recollection activated the hippocampus, and left anterior and inferolateral frontal and parietal cortices more than strong familiarity. In contrast, no brain region that was unaffected by recollection (relative to misses and correct rejections) was modulated by variations in familiarity strength. The implications of these findings for the functional and neural bases of familiarity and recollection are considered. © 2006 Wiley‐Liss, Inc.
Real-time consensus-based scene reconstruction using commodity graphics hardware

We present a novel use of commodity graphics hardware that effectively combines a plane-sweeping algorithm with view synthesis for real-time, on-line 3D scene acquisition and view synthesis. Using real-time imagery from a few calibrated cameras, our method can generate new images from nearby viewpoints, estimate a dense depth map from the current viewpoint, or create a textured triangular mesh. We can do this without prior geometric information or requiring any user interaction, in real time and on line. The heart of our method is using programmable pixel shader technology to square intensity differences between reference image pixels, and then to choose final colors (or depths) that correspond to the minimum difference, i.e. the most consistent color. In this paper we describe the method, place it in the context of related work in computer graphics and computer vision, and present results.
Studies of lysozyme binding to histamine as a ligand for hydrophobic charge induction chromatography

Histamine was immobilized on Sepharose CL‐6B (Sepharose) for use as a ligand of hydrophobic charge induction chromatography (HCIC) of proteins. Lysozyme adsorption onto Histamine‐Sepharose (HA‐S) was studied by adsorption equilibrium and calorimetry to uncover the thermodynamic mechanism of the protein binding. In both the experiments, the influence of salt (ammonium sulfate and sodium sulfate) was examined. Adsorption isotherms showed that HA‐S exhibited a high salt tolerance in lysozyme adsorption. This property was well explained by the combined contributions of hydrophobic interaction and aromatic stacking. The isotherms were well fitted to the Langmuir equation, and the equilibrium parameters for lysozyme adsorption were obtained. In addition, thermodynamic parameters (ΔHads, ΔSads, and ΔGads) for the adsorption were obtained by isothermal titration calorimetry by titrating lysozyme solutions into the adsorbent suspension. Furthermore, free histamine was titrated into lysozyme solution in the same salt‐buffers. Compared with the binding of lysozyme to free histamine, lysozyme adsorption onto HA‐S was characterized by a less favorable ΔGads and an unfavorable ΔSads because histamine was covalently attached to Sepharose via a three‐carbon‐chain spacer. Consequently, the immobilized histamine could only associate with the residues on the protein surface rather than those in the hydrophobic pocket, causing a less favorable orientation between histamine and lysozyme. Further comparison of thermodynamic parameters indicated that the unfavorable ΔSads was offset by a favorable ΔHads, thus exhibiting typical enthalpy‐entropy compensation. Moreover, thermodynamic analyses indicated the importance of the dehydration of lysozyme molecule and HA‐S during the adsorption and a substantial conformational change of the protein during adsorption. The results have provided clear insights into the adsorption mechanisms of lysozyme onto the new HCIC material. © 2009 American Institute of Chemical Engineers Biotechnol. Prog. , 2010
Uptake of offer to receive genetic information about BRCA1 and BRCA2 mutations in an Australian population-based study.

Research on the utilization of genetic testing services for mutations in BRCA1 and BRCA2 has focused on women with a strong family history of breast and ovarian cancer. We conducted a population-based case-control-family study of Australian women diagnosed with invasive breast cancer before age 40 years, unselected for family history, and tested for germ line mutations in BRCA1 and BRCA2. Case subjects found to carry a deleterious mutation and their relatives who had given a research blood sample were informed by mail that the study had identified "genetic information" and were offered the opportunity to learn more. Those interested were referred to a government-funded family cancer clinic. Of 94 subjects who received the letter, 3 (3%) did not respond and 38 (40%) declined to learn their result (16 declined the referral, 10 accepted but did not attend a clinic, and 12 attended a clinic but declined testing), and 12 (13%) remain "on hold. " The remaining 41 (44%) chose to learn their result (3 of whom already knew their mutation status). There was no evidence that the decision to learn of mutation status depended on age, gender, family history, or having been diagnosed with breast cancer. Of 19 families with more than one participant, in 11 (58%) there was discordance between relatives in receiving genetic results. Although in Australia genetic testing is offered free of charge and insurance issues are not a major consideration, we found considerable reluctance, indecision, and unexplained variability both between and within case families in the desire to know their mutation status.
Growth Hormone Stimulates Bone Healing in a Critical-sized Bone Defect Model

Growth hormone plays an important role in bone metabolism. Treating bone deficits is a major topic in orthopaedic surgery. Our hypothesis was that local continuous growth hormone administration stimulates bone healing in a canine critical-sized bone defect model. Bone formation in the defects was quantified using densitometric image analysis and histomorphometry. After growth hormone treatment, expression levels of insulin-like growth factors-I and II, and growth hormone receptor were determined in the bone regenerate of the original defects. Circulating plasma concentrations of insulin-like growth factors-I and II, and insulin- like growth factor binding proteins-4, and 6 were measured during treatment. Growth hormone administration resulted in healing of bone defects but without an additional effect of local infusion. Expression of insulin-like growth factor-I in the bone regenerate was lower in the growth hormonetreated dogs, whereas insulin-like growth factor-II and growth hormone receptor expression were not increased. Growth hormone increased circulating insulin-like growth factor-I and growth factor-II plasma concentrations. Continuous infusion of growth hormone stimulated bone healing in a canine critical-sized bone defect model. Local delivery of growth hormone did not additionally enhance bone healing. Increased circulating plasma concentrations of insulin-like growth factors-I and II most likely induced bone formation.
Morphology of the human internal vertebral venous plexus: A cadaver study after latex injection in the 21–25‐week fetus

The morphology of the anterior and posterior internal vertebral venous plexus (IVVP) in human fetuses between 21–25 weeks of gestational age is described. The results are compared to the findings of a previous morphological study of the IVVP in the aged. The morphological pattern of the anterior IVVP in the fetus is very similar with the anterior IVVP in the aged human. In contrast, the posterior IVVP in the fetus lacks the prominent transverse bridging veins that are present in the aged lower thoracic and the lumbar posterior IVVP. The background of these morphological differences is unclear. Maybe the thoracolumbar part of the posterior IVVP is subject to “developmental delay,” or the observed differences in the aged may result from functional and age‐related factors that trigger this part of the vertebral venous system during (erect) life. The observed age related morphological differences of the posterior IVVP support the concept of the venous origin of the spontaneous spinal epidural hematoma (SSEH). Clin. Anat. 18:397–403, 2005. © 2005 Wiley‐Liss, Inc.
The chemodiversity of wines can reveal a metabologeography expression of cooperage oak wood

Wine chemical compositions, which result from a complex interplay between environmental factors, genetic factors, and viticultural practices, have mostly been studied using targeted analyses of selected families of metabolites. Detailed studies have particularly concerned volatile and polyphenolic compounds because of their acknowledged roles in the organoleptic and therapeutic properties. However, we show that an unprecedented chemical diversity of wine composition can be unraveled through a nontargeted approach by ultrahigh-resolution mass spectrometry, which provides an instantaneous image of complex interacting processes, not easily or possibly resolvable into their unambiguous individual contributions. In particular, the statistical analysis of a series of barrel-aged wines revealed that 10-year-old wines still express a metabologeographic signature of the forest location where oaks of the barrel in which they were aged have grown.
Transient immunosuppressive treatment leads to long-term retention of allogeneic myoblasts in hybrid myofibers

Normal and genetically engineered skeletal muscle cells (myoblasts) show promise as drug delivery vehicles and as therapeutic agents for treating muscle degeneration in muscular dystrophies. A limitation is the immune response of the host to the transplanted cells. Allogeneic myoblasts are rapidly rejected unless immunosuppressants are administered. However, continuous immunosuppression is associated with significant toxic side effects. Here we test whether immunosuppressive treatment, administered only transiently after allogeneic myoblast transplantation, allows the long-term survival of the transplanted cells in mice. Two immunosuppressive treatments with different modes of action were used: (a) cyclosporine A (CSA); and (b) monoclonal antibodies to intracellular adhesion molecule-1 and leukocyte function- associated molecule-1. The use of myoblasts genetically engineered to express beta-galactosidase allowed quantitation of the survival of allogeneic myoblasts at different times after cessation of the immunosuppressive treatments. Without host immunosuppression, allogeneic myoblasts were rejected from all host strains tested, although the relative time course differed as expected for low and high responder strains. The allogeneic myoblasts initially fused with host myofibers, but these hybrid cells were later destroyed by the massive immunological response of the host. However, transient immunosuppressive treatment prevented the hybrid myofiber destruction and led to their long-term retention. Even four months after the cessation of treatment, the hybrid myofibers persisted and no inflammatory infiltrate was present in the tissue. Such long-term survival indicates that transient immunosuppression may greatly increase the utility of myoblast transplantation as a therapeutic approach to the treatment of muscle and nonmuscle disease.
Active Vision : Learning Visual Objects through Egocentric Views of Children and Parents

Work in Cognitive Science has shown that infants are amazingly efficient at the complex task of learning to recognize objects in a world full of visual clutter. In fact, many computer vision researchers have drawn analogies between that process and the impressive recent performance of deep learning. This connection raises the exciting potential that better understanding human learning may give us insight into how to improve deep learning, while deep learning may give us new tools to model and understand how humans learn. We consider a first step towards the latter, in particular using deep learning to test the hypothesis that one reason toddlers are able to learn so efficiently is that they create high-quality visual training data for themselves by actively manipulating objects and thus self-selecting ideal object views for visual learning. We test this idea by collecting egocentric video data of free toy play between toddlers and parents, and then train separate Convolutional Neural Networks based on the toddlers’ views and the parents’ views. Our results show that the egocentric data collected by parents and toddlers have different properties, and that CNNs learn better models using the toddler than the parent views.