Miracle-dz/UniFAR-FTUs huggingface dataset:
Ergosterol Biosynthesis Inhibitors Become Fungicidal when Combined with Calcineurin Inhibitors against Candida albicans, Candida glabrata, and Candida krusei

ABSTRACT Azoles target the ergosterol biosynthetic enzyme lanosterol 14α-demethylase and are a widely applied class of antifungal agents because of their broad therapeutic window, wide spectrum of activity, and low toxicity. Unfortunately, azoles are generally fungistatic and resistance to fluconazole is emerging in several fungal pathogens. We recently established that the protein phosphatase calcineurin allows survival of Candida albicans during the membrane stress exerted by azoles. The calcineurin inhibitors cyclosporine A (CsA) and tacrolimus (FK506) are dramatically synergistic with azoles, resulting in potent fungicidal activity, and mutant strains lacking calcineurin are markedly hypersensitive to azoles. Here we establish that drugs targeting other enzymes in the ergosterol biosynthetic pathway (terbinafine and fenpropimorph) also exhibit dramatic synergistic antifungal activity against wild-type C. albicans when used in conjunction with CsA and FK506. Similarly, C. albicans mutant strains lacking calcineurin B are markedly hypersensitive to terbinafine and fenpropimorph. The FK506 binding protein FKBP12 is required for FK506 synergism with ergosterol biosynthesis inhibitors, and a calcineurin mutation that confers FK506 resistance abolishes drug synergism. Additionally, we provide evidence of drug synergy between the nonimmunosuppressive FK506 analog L-685,818 and fenpropimorph or terbinafine against wild-type C. albicans. These drug combinations also exert synergistic effects against two other Candida species, C. glabrata and C. krusei, which are known for intrinsic or rapidly acquired resistance to azoles. These studies demonstrate that the activity of non-azole antifungal agents that target ergosterol biosynthesis can be enhanced by inhibition of the calcineurin signaling pathway, extending their spectrum of action and providing an alternative approach by which to overcome antifungal drug resistance.
Acquisition of #sC clusters in Spanish-English bilingual children

We evaluate productions of word-initial /s/ clusters (#sC clusters) in English by 40 Spanish-English bilingual children (aged 2;11 – 4;5). These clusters have been studied considerably in research on the phonology of English and related languages because of their unusual patterning. Specifically, #sC clusters violate several phonotactic and/or universal constraints on syllable structure. Monolingual English-speaking children also show production patterns for these clusters that are quite different from those of other clusters. Yet to be fully explored, however, is how these clusters pattern in the speech of English-speaking children who also speak another language that prohibits #sC clusters, such as Spanish. We evaluate Spanish-English bilingual children's productions of #sC clusters to compare their production patterns with the findings of previous research. Results generally indicate that these children's production patterns are consistent with cross-linguistic predictions related to sonority and sonority sequencing. Additionally, some children's productions appeared to be influenced by Spanish phonotactics.
A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)

Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore their use by growers can reduce pesticide residues. Previous studies have reported two glabrous mutant plants containing the genes, csgl1 and csgl2. In the present study, a new glabrous mutant, NCG157, was identified showing a gene interaction effect with csgl1 and csgl2. This mutant showed the glabrous character on stems, leaves, tendrils, receptacles and ovaries, and there were no spines or tumors on the fruit surface. Inheritance analysis showed that a single recessive gene, named csgl3, determined the glabrous trait. An F2 population derived from the cross of two inbred lines 9930 (a fresh market type from Northern China that exhibits trichomes) and NCG157 (an American processing type with glabrous surfaces) was used for genetic mapping of the csgl3 gene. By combining bulked segregant analysis (BAS) with molecular markers, 18 markers, including two simple sequence repeats (SSR), nine insertion deletions (InDel) and seven derived cleaved amplified polymorphism sequences (dCAPs), were identified to link to the csgl3 gene. All of the linked markers were used as anchor loci to locate the csgl3 gene on cucumber chromosome 6. The csgl3 gene was mapped between the dCAPs markers dCAPs-21 and dCAPs-19, at genetic distances of 0.05 cM and 0.15 cM, respectively. The physical distance of this region was 19.6 kb. Three markers, InDel-19, dCAPs-2 and dCAPs-11, co-segregated with csgl3. There were two candidate genes in the region, Csa6M514860 and Csa6M514870. Quantitative real-time PCR showed that the expression of Csa6M514870 was higher in the tissues of 9930 than that of NCG157, and this was consistent with their phenotypic characters. Csa6M514870 is therefore postulated to be the candidate gene for the development of trichomes in cucumber. This study will facilitate marker-assisted selection (MAS) of the smooth plant trait in cucumber breeding and provide for future cloning of csgl3.
Cheshire-cat logic: The recurring theme of terrorist abnormality in psychological research

Abstract Using an encounter from Alice In Wonderland as a metaphor, this article examines the long-running attempt to apply a psychopathology label to terrorists. The disorders of greatest interest to researchers (antisocial, narcissistic and paranoid personality disorders), are described in order to highlight their attraction for theorists. A review of evidence follows. The critique finds that the findings supporting the pathology model are rare and generally of poor quality. In contrast, the evidence suggesting terrorist normality is both more plentiful and of better quality. However, in response to a failure to find any major psychopathology, a trend has emerged which asserts that terrorists possess many of the traits of pathological personalities but do not possess the actual clinical disorders. This development has effectively tainted terrorists with a pathology aura, without offering any way to easily test or refute the accusations.
Ed's Dilemma: Succession Planning at Niagara Paving 1

Ed Merski, the founder of Niagara Paving, a successful asphalt company, must decide whether and on what terms to invite his youngest child Nancy to rejoin the family business. Nancy has worked in a number of jobs in and outside Niagara Paving and soon will graduate from a master of business administration program. Ed is concerned about how her entry will affect not only the operations of the business, but also relations among employees, including his son, Luke, who has worked for the company for the past 20 years.
Accuracy of a cone beam computed tomography-guided surgical stent for orthodontic mini-implant placement.

OBJECTIVE To validate the accuracy of a cone-beam computed tomography (CBCT)-guided surgical stent for orthodontic mini-implant (OMI) placement by quantitatively evaluating the difference between CBCT-prescribed and actual position of mini-implants in preoperative and postoperative CBCT images. MATERIALS AND METHODS A surgical stent was fabricated using Teflon-Perfluoroalkoxy, which has appropriate biological x-ray attenuation properties. Polyvinylsiloxane impression material was used to secure the custom-made surgical stent onto swine mandibles. CBCT scanning was done with the stent in place to virtually plan mini-implants using a three-dimensional (3D) software program. An appropriate insertion point was determined using 3D reconstruction data, and the vertical and horizontal angulations were determined using four prescribed angles. A custom-designed surveyor was used to drill a guide hole within the surgical stent as prescribed on the CBCT images for insertion of 32 OMIs. The mandibles with a surgical stent in place were rescanned with CBCT to measure the deviations between the virtual planning data and surgical results. RESULTS The difference between the prescribed and actual vertical angle was 1.01 ± 7.25, and the horizontal difference was 1.16 ± 6.08. The correlation coefficient confirms that there was no intrarater variability in either the horizontal (R = .97) or vertical (R = .74) vectors. CONCLUSIONS The surgical stent in this study guides mini-implants to the prescribed position as planned in CBCT. Since the statistical difference was not significant, the surgical stent can be considered to be an accurate guide tool for mini-implant placement in clinical use.
Introduction: Emotions in Education

As cognitive, developmental and educational psychologists have moved out of the laboratory and tried to apply their findings to the classroom context, it has become evident that students’ and teachers’ motivational beliefs (i.e., goals, purposes, intentions, interests, self-perceptions of efficacy, control, and competence) influence their learning and behavior. In the 1990s, motivational issues can no longer be ignored by researchers interested in cognition and learning in the classroom. (Pintrich, 1991, p. 199)
Piezoelectric Bimorphs' Characteristics as In-Socket Sensors for Transfemoral Amputees

Alternative sensory systems for the development of prosthetic knees are being increasingly highlighted nowadays, due to the rapid advancements in the field of lower limb prosthetics. This study presents the use of piezoelectric bimorphs as in-socket sensors for transfemoral amputees. An Instron machine was used in the calibration procedure and the corresponding output data were further analyzed to determine the static and dynamic characteristics of the piezoelectric bimorph. The piezoelectric bimorph showed appropriate static operating range, repeatability, hysteresis, and frequency response for application in lower prosthesis, with a force range of 0–100 N. To further validate this finding, an experiment was conducted with a single transfemoral amputee subject to measure the stump/socket pressure using the piezoelectric bimorph embedded inside the socket. The results showed that a maximum interface pressure of about 27 kPa occurred at the anterior proximal site compared to the anterior distal and posterior sites, consistent with values published in other studies. This paper highlighted the capacity of piezoelectric bimorphs to perform as in-socket sensors for transfemoral amputees. However, further experiments are recommended to be conducted with different amputees with different socket types.
The evolution of liver disease in cystic fibrosis

OBJECTIVES To describe prospectively the evolution of liver abnormalities in cystic fibrosis (CF), and to assess their impact on nutritional status. STUDY DESIGN 124 children (61 boys) with CF (median age, 5.4 years; range, 0.1–13.9) were followed longitudinally for a median of four years. Annual clinical examination, biochemistry, and ultrasound assessment were performed. Chrispin-Norman score, anthropometry, and bacterial colonisation of airway secretions were measured at each assessment. RESULTS At initial assessment, 45% of the patients had no liver abnormalities, 42% had biochemical abnormality, 35% ultrasound abnormality, and 6% had clinical abnormality of the liver. In this cross sectional analysis, abnormal biochemistry was present in 40% of children with ultrasound or clinical abnormalities, but when longitudinal follow up data were analysed, abnormal biochemistry preceded or coincided with abnormal ultrasound or clinical hepatosplenomegaly in three quarters of 53 children developing new abnormalities. Eighty four of 124 children (68%) showed ultrasound or clinical evidence of liver abnormality at some point during the four years of follow up. No association was found between liver disease and nutritional status. CONCLUSIONS Hepatic abnormality was common in this group of children with CF, was often predicted by intermittent biochemical abnormalities, and was not associated with deterioration in nutritional status.
Chronic levodopa therapy does not improve skilled reach accuracy or reach range on a pasta matrix reaching task in 6‐OHDA dopamine‐depleted (hemi‐Parkinson analogue) rats

l‐dopa therapy reverses some but not all of the motor deficits in human Parkinson patients. Although a number rat analogues of human Parkinson's disease have been developed for evaluating the efficacy of drug therapies, it is not known whether l‐dopa has a similar selective action on the motor symptoms in the rat models. To examine the effectiveness of l‐dopa in reversing the motor deficits in rats, we administered 6‐OHDA unilaterally to produce hemi‐Parkinson rats, which were then trained to reach for food using either their impaired (contralateral to the lesion) limb or their good (ipsilateral to the lesion) limb. To assess the skill, accuracy and range of limb movement, rats reached for pasta from a horizontal array of 260 vertically orientated pieces of pasta. The number and location of pasta pieces taken from this matrix was calculated and the qualitative aspects of the reaching movements were rated. The quantitative data on pasta sticks retrieved indicated that forelimb extension and movement radius around the shoulder joint was reduced by 6‐OHDA treatment and did not improve after chronic l‐dopa treatment. The qualitative analysis showed that grasping patterns, paw movements and body movements impaired by the lesion were also not improved by l‐dopa treatment. These findings are the first in the rat to suggest that whereas l‐dopa has a general activating effect on the rat's whole‐body movements, as displayed in contralateral rotation, its effectiveness does not extend to skilled forelimb movements. The results are discussed in relationship to the idea that the restoration of some skilled movements may require normal synaptic function.
How can leaders achieve high employee engagement

Purpose – Organizations aspire to have engaged employees, and spend considerable resources to measure and improve employee engagement. Theoretically, leadership is a key antecedent of engagement, yet there is no research directly linking leader behaviors and follower engagement. The purpose of this paper is to investigate the evidence for such a link.Design/methodology/approach – Research was conducted with a large New Zealand insurance organization, using data from direct reports. A pilot study was first conducted (n=236), in which the JRA 360‐degree feedback instrument was factor analyzed. Subsequently, a linkage analysis (n=178) was conducted to establish the relationship of the resultant leadership factors with JRA's employee engagement measure.Findings – Three factors emerged from the JRA 360: supports team, performs effectively, and displays integrity. Correlation and regression results showed that supports team was the strongest predictor of engagement; semi‐partial correlations showed that the thr.
The inorganic biochemistry of molybdoenzymes

Molybednum-containing enzymes (Coughlan, 1980; Spiro, 1985) occupy a significant place in the development of the field now termed inorganic biochemistry. The importance of the metal as a biological trace element depends on its involvement in the known, and perhaps other as yet unknown, molybdoenzymes. That it plays a role in biological nitrogen fixation, the process whereby the enzyme nitrogenase in the root nodules of plants converts atmospheric nitrogen into ammonia, was recognized in the 1930s. The metal is also a constituent of a variety of other enzymes, having first been found in a mammalian enzyme, xanthine oxidase, in the 1950s.
Personality disorder scale predictors of depression stability over time as a partial function of mental health history

The high comorbidity of personality disturbance and psychiatric symptomatology has been well established. Diagnostic and Statistical Manual-IV (DSM-IV) personality disorder symptom clusters often represent aberrant, intense and labile emotional reactions to stressors. The role of personality disorder traits on the variability of depression symptoms as expressed over time, however, has gained relatively little research attention. The presence and number of personality disorder diagnoses have been associated with earlier depression onset and less favourable treatment outcomes suggesting that this form of mood disturbance may be more durable over time when associated with Axis II features. The present study examined Beck Depression Inventory (BDI) temporal stability as a function of Millon Clinical Multiaxial Inventory (MCMI-II) personality disorder base rate scores among 406 college students with and without reported histories of significant mental health concerns. Instability of BDI scores across time was shown to be predicted (r = 0.15) by selected personality disorderscale dimensions (antisocial, self-defeating, borderline and total number of MCMI-II personality disorder elevations). BDI reliability did drop significantly among participants reporting a mental health treatment history and multiple personality disorder elevations. Gender differences were not found in the strength of these bivariate correlations. Women generated smaller BDI absolute differences than men. While BDI test–retest reliability was only linked modestly to personality disorder attributes in this college sample, further study may be warranted to evaluate similar relationships within a clinical sample. Copyright © 2009 John Wiley & Sons, Ltd.
An Empirical Study of Empowerment in the Workplace

Empowerment in the workplace was developed and practiced in the 1950s and 1960s. Recent forces in the business community, such as globalization, skilled staff shortages, and the need for innovation and productivity have added momentum to the search for ways to survive. Despite limited empirical evidence, empowerment has been advocated as a promising solution. This study evaluates the effect of a controlled introduction of empowerment, with reference to power, managerial functions, leadership styles, and employee motivation. Data from 81 employees who were formally empowered were compared with 90 individuals in a control group. Results provided minimal support for the positive influence of empowerment. The disruptive nature of corporate downsizing that occurred during the introduction of empowerment may have nullified its effect.
BUDGET ANALYSIS AND POLICY ADVOCACY: THE ROLE OF NON-GOVERNMENTAL PUBLIC ACTION

This paper examines the impact of independent budget analysis and advocacy initiatives that are designed to improve budget transparency and government expenditure priorities. It draws on six case studies of independent budget groups in Brazil, Croatia, India, Mexico, South Africa, and Uganda, which include non-governmental organisations, research institutions and social movements. The findings demonstrate that the most significant impacts achieved by independent budget groups include improvements in the transparency of budgetary decisions and the budget process; increased budget awareness and literacy; and enhanced engagement in the budget process on the part of legislators, the media and civil society organisations. While the structure of the budget process makes substantial changes in expenditure priorities difficult to achieve, budget groups can directly contribute to positive impacts on budget allocations and implementation. Tracking of budgetary expenditures and impacts was also found to be effective in ensuring effective utilisation of education and health expenditures. Increased budget allocations and improved utilisation of public funds that benefit poor and disadvantaged groups can ensure greater equity in budget priorities and further social justice objectives.
Genetic Profiles of Korean Patients With Glucose-6-Phosphate Dehydrogenase Deficiency

Background We describe the genetic profiles of Korean patients with glucose-6-phosphate dehydrogenase (G6PD) deficiencies and the effects of G6PD mutations on protein stability and enzyme activity on the basis of in silico analysis. Methods In parallel with a genetic analysis, the pathogenicity of G6PD mutations detected in Korean patients was predicted in silico. The simulated effects of G6PD mutations were compared to the WHO classes based on G6PD enzyme activity. Four previously reported mutations and three newly diagnosed patients with missense mutations were estimated. Results One novel mutation (p.Cys385Gly, labeled G6PD Kangnam) and two known mutations [p.Ile220Met (G6PD São Paulo) and p.Glu416Lys (G6PD Tokyo)] were identified in this study. G6PD mutations identified in Koreans were also found in Brazil (G6PD São Paulo), Poland (G6PD Seoul), United States of America (G6PD Riley), Mexico (G6PD Guadalajara), and Japan (G6PD Tokyo). Several mutations occurred at the same nucleotide, but resulted in different amino acid residue changes in different ethnic populations (p.Ile380 variant, G6PD Calvo Mackenna; p.Cys385 variants, Tomah, Madrid, Lynwood; p.Arg387 variant, Beverly Hills; p.Pro396 variant, Bari; and p.Pro396Ala in India). On the basis of the in silico analysis, Class I or II mutations were predicted to be highly deleterious, and the effects of one Class IV mutation were equivocal. Conclusions The genetic profiles of Korean individuals with G6PD mutations indicated that the same mutations may have arisen by independent mutational events, and were not derived from shared ancestral mutations. The in silico analysis provided insight into the role of G6PD mutations in enzyme function and stability.
Beyond Repair: Conversation Analysis as an Approach to SLA.

As one of several approaches to SLA as social practice, Conversation Analysis (CA) has the capacity to examine in detail how opportunities for L2 learning arise in different interactional activities. Its particular strength, and one that distinguishes it from other social practice approaches, is its consistent focus on the orientations and relevancies that participants display to each other through their interactional conduct. CA thus affords a distinct perspective on L2 learning as object and process. It enables researchers to reconsider such established SLA topics as fluency, correction, or the benefits of tasks for L2 learning, but also offers an acquisitional perspective on interactional conduct whose potential for L2 learning has been largely unexplored.
Supported Employment in New York State: A Comparison of Benefits and Costs

The rapid growth of supported employment programs in the last five years has been accompanied by the strong belief that through such programs many people with severe disabilities would be more satisfied with their lives and receive higher earnings than in sheltered workshops, work activity centers, and adult day care. This paper presents data collected in New York State that test these propositions and compare the benefits with the costs of operating the supported employment program.