Miracle-dz/UniFAR-FTUs huggingface dataset:
Capability of B-type natriuretic peptide (BNP) and amino-terminal proBNP as indicators of cardiac structural disease in asymptomatic patients with systemic arterial hypertension.

BACKGROUND The aim of the present study was to prospectively evaluate the diagnostic utility of B-type natriuretic peptide (BNP) and amino-terminal proBNP (NT-proBNP) measurements for the detection of cardiac structural disease in asymptomatic patients with systemic arterial hypertension and to test the hypothesis that the 2 analytes are equally useful in this clinical setting. METHODS We studied a consecutive series of 149 asymptomatic patients referred for echocardiographic evaluation of the cardiac effects of systemic arterial hypertension. Diagnosis of cardiac structural disease was based on the presence of systolic or diastolic dysfunction, left atrial dilatation, left ventricular dilatation or hypertrophy, pulmonary hypertension, and wall motion or valvular abnormalities. Blood concentrations of BNP and NT-proBNP were measured by 2 commercially available assays (Abbott AxSYM and Roche Elecsys, respectively). Diagnostic accuracies of BNP and NT-proBNP were assessed by ROC curve analysis. Areas under the curves were compared by analysis of equivalency. RESULTS In distinguishing between hypertensive patients with cardiac structural disease (n = 118) and hypertensive patients without (n = 31), areas under the curves were 0.740 (95% confidence interval, 0.662-0.808) for BNP and 0.762 (0.685-0.828) for NT-proBNP and were significantly equivalent (P = 0.015). Cutoff values with a 90% sensitivity for cardiac structural disease were 17 ng/L for BNP and 39 ng/L for NT-proBNP, with 29% and 32% specificity, respectively. CONCLUSIONS BNP and NT-proBNP have similar capabilities for detecting cardiac structural disease in asymptomatic patients with systemic arterial hypertension. However, in the setting evaluated, a screening strategy relying on measurement of BNP or NT-proBNP may be of limited value because of the low specificity at the selected cutoff values.
Combining Multiple Knowledge Sources for Dialogue Segmentation in Multimedia Archives

Automatic segmentation is important for making multimedia archives comprehensible, and for developing downstream information retrieval and extraction modules. In this study, we explore approaches that can segment multiparty conversational speech by integrating various knowledge sources (e.g., words, audio and video recordings, speaker intention and context). In particular, we evaluate the performance of a Maximum Entropy approach, and examine the effectiveness of multimodal features on the task of dialogue segmentation. We also provide a quantitative account of the effect of using ASR transcription as opposed to human transcripts.
Quantitative Analysis of Cumulative Trauma Risk Factors and Risk Factor Interactions

This study identifies the strongest occupational risk factors and risk factor interactions in the development of indicators associated with cumulative trauma disorders (CTD). Suggestions for minimizing the occurrence of indicators associated with onset of CTD are presented. Five occupational risk factors and six risk factor interactions were evaluated to determine their contribution to accepted indicators of the onset of CTD, including exertion, discomfort, difficulty, and fatigue. Taguchi’s level-analysis procedure was used to design a 16-trial hammering experiment and to analyze the results. From the hypothesized occupational risk factors, repetition was found to have the strongest effect on the values of the physiological and subjective indicators of the onset of CTD.
Project SCORE! Coaches’ perceptions of an online tool to promote positive youth development in sport

Research points to the potential of youth sport as an avenue to support the growth of particular assets and outcomes. A recurring theme in this line of research is the need to train coaches to deliberately deliver themes relating to positive youth development (PYD) consistently in youth sport programs. The purpose of the study was to design and deliver a technology-based PYD program. Project SCORE! (www.projectscore.ca) is a series of 10 lessons to help coaches integrate PYD into sport. Four youth sport coaches completed the program in this first phase of this research and were interviewed. The goal of this study was to gain some insights from coaches as they completed the program. Positive comments about the program (i.e. ease of use, success of particular lessons, coach’s personal growth) and challenges regarding teaching positive skills to youth are discussed. These results helped to shape the program and make necessary changes so that it may be used for a larger research study. Other implications and future research directions are discussed.
Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis

Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well “U” bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.
Inhibition by bradykinin of voltage-activated barium current in a rat dorsal root ganglion cell line: role of protein kinase C

The whole-cell patch-clamp technique was used to record Ba2+ currents through voltage-activated calcium channels in the clonal dorsal root ganglion cell line F11-B9. The pain-producing peptide bradykinin (BK; 100 nM) reduced the sustained Ba2+ current in F11-B9 cells by 30%. In cultures prelabeled with 3H-arachidonic acid and tested under ionic conditions similar to those used for recording Ba2+ currents, BK also induced a concentration-dependent, transient, 2.7-fold accumulation of 3H-diacylglycerol. Both the elevation of 3H-diacylglycerol and the inhibition of Ba2+ current began within 5 sec following BK exposure, and the effective concentration range of BK was similar for the 2 responses. In whole-cell recordings, extracellularly applied 1-oleoyl-2- acetylglycerol (OAG; 0.5–5 microM) mimicked the degree of block and occluded the block of sustained current by BK. Another protein kinase C (PKC) activator, 1,2-dioctanoylglycerol (diC8), blocked 70–100% of sustained current when applied intracellularly or extracellularly at 5 microM, whereas extracellular application of ethylene glycol dioctanoate (5 microM), an analog reported not to stimulate PKC, inhibited only 14% of sustained current. The pseudosubstrate peptide PKC19–36 (2 microM in pipette) and the lipid staurosporine (100 nM in pipette), both inhibitors of PKC, reduced the effects of maximal concentrations of OAG or BK by 55–60%. Dynorphin A applied intracellularly (2 microM) as a control for nonspecific effects of PKC19–36 did not inhibit the block of sustained current by BK. These data are consistent with the hypothesis that BK inhibits whole-cell sustained Ba2+ current in F11-B9 cells via a mechanism that involves activation of PKC.
Learning to Navigate in Complex Environments

Learning to navigate in complex environments with dynamic elements is an important milestone in developing AI agents. In this work we formulate the navigation question as a reinforcement learning problem and show that data efficiency and task performance can be dramatically improved by relying on additional auxiliary tasks leveraging multimodal sensory inputs. In particular we consider jointly learning the goal-driven reinforcement learning problem with auxiliary depth prediction and loop closure classification tasks. This approach can learn to navigate from raw sensory input in complicated 3D mazes, approaching human-level performance even under conditions where the goal location changes frequently. We provide detailed analysis of the agent behaviour, its ability to localise, and its network activity dynamics, showing that the agent implicitly learns key navigation abilities.
Public Perceptions of the Media Coverage of Irregular Immigration

Irregular immigration has become a globally important topic. While there have been some studies on public opinion toward irregular immigration, virtually no studies have examined how audiences evaluate the media coverage of this issue. There is also a lack of comparative research. The aims of this article are to provide survey data on public opinion toward irregular immigration in the United States, France, and Norway as well as a comparative analysis of public perceptions of the news coverage. Findings suggest that irregular immigration remains a highly salient issue in public opinion in all three countries. Furthermore, public opinion is generally critical and skeptic toward irregular immigration and immigrants, and differences between countries regarding the coverage of the issue in national mainstream media do not necessarily seem to be mirrored in public opinion. The survey data also suggest that citizens in all three countries tend to believe that the negative aspects of irregular immigration such as crimes or border control receive too little coverage whereas perspectives more positive to irregular immigration receive too much. Implications for further comparative research on public opinion and media coverage are discussed.
High Concordance of Gene Expression Profiling–correlated Immunohistochemistry Algorithms in Diffuse Large B-cell Lymphoma, Not Otherwise Specified

Diffuse large B-cell lymphoma (DLBCL) is classified into prognostically distinct germinal center B-cell (GCB) and activated B-cell subtypes by gene expression profiling (GEP). Recent reports suggest the role of GEP subtypes in targeted therapy. Immunohistochemistry (IHC) algorithms have been proposed as surrogates of GEP, but their utility remains controversial. Using microarray, we examined the concordance of 4 GEP-correlated and 2 non–GEP-correlated IHC algorithms in 381 DLBCLs, not otherwise specified. Subtypes and variants of DLBCL were excluded to minimize the possible confounding effect on prognosis and phenotype. Survival was analyzed in 138 cyclophosphamide, adriamycin, vincristine, and prednisone (CHOP)-treated and 147 rituximab plus CHOP (R-CHOP)-treated patients. Of the GEP-correlated algorithms, high concordance was observed among Hans, Choi, and Visco-Young algorithms (total concordance, 87.1%; &kgr; score: 0.726 to 0.889), whereas Tally algorithm exhibited slightly lower concordance (total concordance 77.4%; &kgr; score: 0.502 to 0.643). Two non–GEP-correlated algorithms (Muris and Nyman) exhibited poor concordance. Compared with the Western data, incidence of the non-GCB subtype was higher in all algorithms. Univariate analysis showed prognostic significance for Hans, Choi, and Visco-Young algorithms and BCL6, GCET1, LMO2, and BCL2 in CHOP-treated patients. On multivariate analysis, Hans algorithm retained its prognostic significance. By contrast, neither the algorithms nor individual antigens predicted survival in R-CHOP treatment. The high concordance among GEP-correlated algorithms suggests their usefulness as reliable discriminators of molecular subtype in DLBCL, not otherwise specified. Our study also indicates that prognostic significance of IHC algorithms may be limited in R-CHOP-treated Asian patients because of the predominance of the non-GCB type.
Azlocillin: In Vitro Studies of a New Semisynthetic Penicillin

The activity of azlocillin, a new semisynthetic penicillin, was determined against 582 clinical isolates of gram-negative bacilli and gram-positive cocci. Over 75% of the isolates of Pseudomonas aeruginosa were inhibited at a concentration of 12.5 μg or less per ml. Azlocillin is also active against indole-negative and -positive Proteus spp. , inhibiting 98 and 71%, respectively, at a concentration of 12.5 μg or less per ml. Isolates of Klebsiella spp. and Enterobacter spp. showed less susceptibility than isolates of Escherichia coli and Serratia spp. Gram-positive cocci except penicillin G-resistant Staphylococcus aureus were susceptible to azlocillin. Azlocillin failed to inhibit the growth of gram-negative bacilli when large inocula were used. It was more active in alkaline pH, but the type of medium used had little effect on its activity. Azlocillin was more active than mezlocillin, ticarcillin, and carbenicillin and as active as BLP-1654 against isolates of P. aeruginosa. It was not as active as mezlocillin against the majority of the other gram-negative bacilli.
Seminal plasma characteristics during frequent ejaculation.

Seven young men provided a semen sample every 8 h for 2 days. Citric acid, zinc, acid phosphatase , spermine, fructose and protein were measured in the samples. Despite a dramatic reduction in semen volume, the concentration of the majority of the parameters remained constant during the period of study. The protein profile of the ejaculates, as assessed by 2-dimensional electrophoresis, also did not change. Levels of citric acid, zinc and acid phosphatase were closely correlated, while spermine, which is also believed to be of prostatic origin, showed no correlation with these other parameters. The spermine concentration of the ejaculates increased significantly during the period of high ejaculation frequency and returned to normal levels after a 3 day abstinence period. In view of the fact that spermine accumulation in tissues appears to be associated with growth and protein synthesis this increase in seminal spermine may reflect a stimulation of prostatic cell activity.
Sensing Informal Networks in Organizations

We present an examination of informal network structure within the sales division of a global manufacturing organization. Sociometric Badges were used to collect data on face-to-face interactions over a total of 8 weeks, the latter half of which was spent in a redesigned workspace. These data were supplemented by employees’ e-mail and instant messaging log activity. The allocation of an individual’s communication among colleagues reflected the company’s structure as a post-bureaucratic organization. The observed interteam communication patterns differed from those expected to arise based on the various functions performed by each team throughout the sales cycle, suggesting that the communication needs of each team were not wholly provided for by the available media. A subset of workers who were encouraged to utilize flexible seating arrangements in a remodeled space had a higher proportion of face-to-face interactions with colleagues outside of their team, while employees seated far away from each other were less likely to exchange e-mail. This research has implications for companies hoping to understand the structure of informal networks within their organization as well as those considering workplace redesign as a method of stimulating communication within these networks.
High Trait Anger Mexican Youth: Characteristics, Parental Anger, and Counseling Needs

Abstract This study compared three groups of Mexican youth: (1) high trait anger adolescents recognizing anger problems (HR); (2) high trait anger youth not reporting anger problems (HNR); and (3) low trait anger adolescents not reporting anger problems (LNR). The HR group was sizable, representing 21% of all students and 72% of high anger youth. Compared to LNR, high anger groups (HR and HNR) experienced more angry feelings, engaged in anger suppression (e.g., holding anger in and harboring grudges) and aggressive anger expression (e.g., urges to aggression, physical aggressive anger expression toward others and toward self and objects), and reported lower internal and external anger control (e.g., relaxing and controlling one’s behavior when angry). High anger groups also reported greater trait anger in both parents than LNR, suggesting parent’s anger is a risk factor for anger in adolescents. HR and HNR groups, however, did not differ on any variable. Findings for high anger groups supported the intensity, aggression, and reduced positive coping hypotheses of State-Trait Anger Theory. Findings were also discussed in terms of the counseling needs of high anger Mexican youth and State-Trait Theory.
Isolation and genotyping of Acanthamoeba strains from corneal infections in Italy.

Acanthamoeba keratitis (AK) is a corneal disease caused by members of a genus of free-living amoebae and is associated predominantly with contact lens (CL) use. This study reports 16 cases of culture-proven AK diagnosed in northern Italy. Genotype identification was carried out with a PCR assay based on sequence analysis of the 18S rRNA gene, and sensitivity and specificity were evaluated in comparison with traditional parasitological techniques. A 405 bp region of the 18S rRNA gene (ASA.S1) including diagnostic fragment 3 (DF3) was amplified using the genus-specific primers JDP1 and JDP2. Genotype assignment was based on phenetic analysis of the ASA.S1 subset of the nuclear small-subunit rRNA gene sequence excluding the highly variable DF3 region. Phylogenetic analysis was also performed on the sequences obtained. All patients complained of monolateral infection; 11 (68.75%) admitted improper CL disinfection. In 14/16 (87.5 %) subjects, corneal scrapings were stained with calcofluor white and haematoxylin and eosin and, in ten cases (62.5 %), microscopy was positive for Acanthamoeba cysts. In vitro culture on 3 % non-nutrient agar plates was obtained in all cases (100 %), whereas cloning and axenic growth were positive for 14 amoebic stocks (87.5 %). PCR analysis had 100 % sensitivity and specificity compared with in vitro axenic culture, showing positive amplification from 15 isolates. All Acanthamoeba strains belonged to the T4 genotype, the main AK-related genotype worldwide. These results confirmed the importance of a complete diagnostic protocol, including a PCR assay, for the clinical diagnosis of AK on biological samples. Genotyping allowed inclusion of all isolates in the T4 group, thus demonstrating the prevalence of this genotype in northern Italy.
Regionally Specific and Genome-Wide Analyses Conclusively Demonstrate the Absence of CpG Methylation in Human Mitochondrial DNA

ABSTRACT Although CpG methylation clearly distributes genome-wide in vertebrate nuclear DNA, the state of methylation in the vertebrate mitochondrial genome has been unclear. Several recent reports using immunoprecipitation, mass spectrometry, and enzyme-linked immunosorbent assay methods concluded that human mitochondrial DNA (mtDNA) has much more than the 2 to 5% CpG methylation previously estimated. However, these methods do not provide information as to the sites or frequency of methylation at each CpG site. Here, we have used the more definitive bisulfite genomic sequencing method to examine CpG methylation in HCT116 human cells and primary human cells to independently answer these two questions. We found no evidence of CpG methylation at a biologically significant level in these regions of the human mitochondrial genome. Furthermore, unbiased next-generation sequencing of sodium bisulfite treated total DNA from HCT116 cells and analysis of genome-wide sodium bisulfite sequencing data sets from several other DNA sources confirmed this absence of CpG methylation in mtDNA. Based on our findings using regionally specific and genome-wide approaches with multiple human cell sources, we can definitively conclude that CpG methylation is absent in mtDNA. It is highly unlikely that CpG methylation plays any role in direct control of mitochondrial function.
Acceptance of Wireless Internet via Mobile Technology in China

This study explores factors significantly impact the acceptance of Wireless Internet via Mobile Technology (WIMT) in China. The results indicate that the acceptance of WIMT is related with factors of: perceived usefulness, perceived ease of use, social influences, wireless trust environment, and facilitating conditions. Factors of perceived usefulness and ease of use are directed by system complexity. This study provides diagnostic insight into how different factors influence user intention to accept WIMT and thus helps business build solid strategy to prompt WIMT and m-commerce in China.
Antecedents of team turnover intentions in temporary organizations: Development of a research model

Purpose – The purpose of this paper is to shed light on the antecedents of team turnover intentions in temporary organizations such as projects, programs, or temporary teams. Design/methodology/approach – The authors systematically combine the findings from the literature on the antecedents of turnover intentions in permanent organizations (PO) with the characteristics of temporary organizations (TO) and develop a research model comprising personal factors, job-related factors, and organization-related factors. Findings – A research model consisting of testable propositions that the authors derived from both specificities of TO and the antecedents of turnover intentions in PO. Originality/value – The authors contribute to literature by identifying potential antecedents of turnover intentions that are specific to TOs and those that also exist in POs but are adapted to a temporary context. The research model allows future empirical research on turnover in TO to be conducted in a systematic way and supports .