Miracle-dz/UniFAR-FTUs huggingface dataset:
Blue-emitting ZnSe random laser

We demonstrate a blue-emitting semiconductor random laser using a thin ZnSe powered film at room and low temperatures. Below the threshold excitation, we observe a broad spontaneous emission band centered at ~470 nm due to the excitonic transitions in ZnSe particles. Above the threshold excitation, several discrete lasing lines caused by the light amplification in closed-loop paths and emitted in all directions were observed near the center of the spontaneous emission band (~475 nm). The linewidth of each discrete lasing line is less than 0.4 nm. Lasing threshold power density is determined to be ~700 kW/cm2. The effective cavity diameter Deff is also estimated to be ~6 μm from the experimental lasing spectrum by performing Fourier transform analysis. The temperature dependence of the lasing characteristics in ZnSe random laser is also investigated and found that decreasing temperature shifts the discrete lasing lines toward higher-energy side with increasing their intensities.
The Fear of Pain Questionnaire-III and the Fear of Pain Questionnaire-Short Form: a confirmatory factor analysis

Background The Fear of Pain Questionnaire-III (FPQ-III) is a widely used instrument to assess the fear of pain (FOP) in clinical and nonclinical samples. The FPQ-III has 30 items and is divided into three subscales: Severe Pain, Minor Pain and Medical Pain. Due to findings of poor fit of the original three-factor FPQ-III model, the Fear of Pain Questionnaire-Short Form (FPQ-SF) four-factor model has been suggested as an alternative. The FPQ-SF is a revised version of the FPQ-III, reduced to 20 items and subdivided into four subscales: Severe Pain, Minor Pain, Injection Pain and Dental Pain. Aims and methods The purpose of the study was to investigate the model fit, reliability and validity of the FPQ-III and the FPQ-SF in a Norwegian nonclinical sample, using confirmatory factor analysis (CFA). The second aim was to explore the model fit of the two scales in male and female subgroups separately, since previous studies have uncovered differences in how well the questionnaires measure FOP across sex; thus, the questionnaires might not be sex neutral. It has been argued that the FPQ-SF model is better because of the higher fit to the data across sex. To explore model fit across sex within the questionnaires, the model fit, validity and reliability were compared across sex using CFA. Results The results revealed that both models’ original factor structures had poor fit. However, the FPQ-SF had a better fit overall, compared to the FPQ-III. The model fit of the two models differed across sex, with better fit for males on the FPQ-III and for females on the FPQ-SF. Conclusion The FPQ-SF is a better questionnaire than the FPQ-III for measurement of FOP in Norwegian samples and across sex subgroups. However, the FPQ-III is a better questionnaire for males than for females, whereas the FPQ-SF is a better questionnaire for females than for males. The findings are discussed and directions for future investigations outlined.
Expression of Nodal, Cripto, SMAD3, Phosphorylated SMAD3, and SMAD4 in the Proliferative Endometrium of Women With Endometriosis

Background: Nodal is a growth factor of the transforming growth factor β superfamily that is expressed in high turnover tissues, such as the human endometrium, and in several malignancies. The effects of Nodal are modulated by the coreceptor Cripto and mediated by SMAD proteins. This study evaluated the gene and protein expression of Nodal, Cripto, total and phosphorylated (p) SMAD3, and SMAD4 in the proliferative endometrium of women with and without endometriosis. Method: Total RNA was isolated and complementary DNA synthesized from eutopic endometrium of women with (n = 15) and without (n = 12) endometriosis, followed by quantitative real-time polymerase chain reaction (PCR) to evaluate the gene expression of Nodal, Cripto, SMAD3, and SMAD4. Western blot was used to evaluate the protein levels of Nodal and Cripto, and immunohistochemistry was performed to localize SMAD3, pSMAD3, and SMAD4. Results: Although Nodal expression was unchanged in women with endometriosis, real-time PCR indicated lower gene expression of Cripto (fold change 0.27, P < .05) in the endometriosis group. This difference, however, was not maintained at protein expression level as assessed by Western blot. The immunostaining of total SMAD3 was reduced in the endometriosis group (P < .01), but the localization of pSMAD3 and the nuclear staining of SMAD4 were unchanged. Conclusion: These findings suggest that the Nodal signaling pathway has subtle changes in the endometrium of women with endometriosis, but this imbalance may not cause functional damage as it seems not to affect the nuclear expression of SMAD4.
Structure solution of the basic decagonal Al-Co-Ni phase by the atomic surfaces modelling method.

The atomic surfaces modelling technique has been used to solve the structure of the basic Ni-rich Al-Co-Ni decagonal phase. Formula Al70.6Co6. 7Ni22.7, space group P10, five-dimensional unit-cell parameters: d1 = d4 = 4.752 (3) A, d2 = d3 = 3.360 (2) A, d5 = 8.1710 (2) A; alpha12 = alpha34 = 69.295 degrees, alpha13 = alpha24 = 45 degrees, alpha14 = 41.410 degrees, alpha23 = alphai5 = 90 degrees (i = 1-4), V = 291.2 (7) A5; D(x) = 3.887 Mg x m(-3). Refinement based on /F/; 2767 unique reflections (/F/ > 0), 749 parameters, R = 0.17, wR = 0.06. Describing the structure of quasicrystals embedded in n-dimensional superspace in principle takes advantage of n-dimensional periodicity to select the minimal set of degrees of freedom for the structure. The method of modelling of the atomic surfaces yielded the first fully detailed structure solution of this phase. Comparison with numerous former, less accurate models confirms several features already derived, but adds a new essential insight of the structure and its complexity. The atoms fill the space forming recurrent structure motifs, which we will (generically) refer to as clusters. However, no unique cluster exists, although differences are small. Each cluster shows a high degree of structural disorder. This gives rise to a large configurational entropy, as much as expected in a phase which is stable at high temperature. On the other side, the cluster spatial arrangement is perfectly quasiperiodic. These considerations, corroborated by analysis of the structural relationship with neighbouring periodic phases, strongly suggest the existence of a non-local, long-range interaction term in the total energy which may be essential to the stability.
Suicidal Behavior in Prodromal Huntington Disease

Background: Several studies have suggested a greater risk of suicide in Huntington disease (HD); however, unique risk factors for suicide in HD are not established. Objective: We sought to determine risk factors for suicidal behavior, defined as suicide or attempted suicide, in prodromal HD. Methods: From the prospective PREDICT-HD cohort, we identified 735 cases with HD gene expansion but no manifest symptoms of HD and 194 non-gene-expanded controls. In survival analysis, a number of potential risk factors for suicidal behavior were assessed, including symptoms of depression, hopelessness, substance abuse, marital status, gender, and psychiatric history. Results: During a mean of 3.7 years of prospective follow-up, 12 cases (1.6%) attempted suicide and 1 completed suicide (0.1%). No suicides were observed among controls. In univariate Cox proportional hazards regression models, a history of suicide attempts (HR 8.5, 95% CI 2.8–26.1, p < 0.0002) and a Beck Depression Inventory II score >13 (HR 7.2, 95% CI 2.3–22.0, p < 0.0006) were associated with suicidal behavior. These risk factors had independent effects in multivariate models. A history of incarceration in the past 2 years was also associated (HR 12.5, 95% CI 2.7–56.6, p < 0.002), though uncommon. No further risk factors were identified. Conclusion: A history of suicide attempts and the presence of depression are strongly predictive of suicidal behavior in prodromal HD. As these risk factors are among the most robust risk factors for suicide, established suicide risk factors appear applicable to those with prodromal HD.
Mice Deficient in Telomerase Activity Develop Hypertension Because of an Excess of Endothelin Production

Background— Telomere shortening has been related to vascular dysfunction and hypertension. In the present study, we analyzed the influence of telomerase deficiency and telomere shortening on arterial pressure (AP). Methods and Results— AP was evaluated in 6-month-old mice lacking the RNA component of the telomerase (terc−/−) at the first generation and third generation (G3). First generation and G3 mice showed higher AP than wild-type (WT) mice. To analyze the mechanisms involved, mean AP and vascular resistance in response to vasoactive substances were measured in G3 and WT mice. These mice showed similar responses to acetylcholine, NG-nitro-l-arginine methyl ester, angiotensin II, and losartan administration. Mean AP did not increase after endothelin-1 (ET-1) administration in G3 mice, but it did in WT animals. Bosentan treatment decreased mean AP only in G3 mice. Serum and urine concentrations of ET-1 were higher in terc−/− than in WT mice. Endothelin-converting enzyme (ECE-1) mRNA expression was higher in terc−/− animals than in the WT group. FR901533, an ECE antagonist, decreased blood pressure in conscious G3 mice. Studies in mouse embryonic fibroblasts from G3 mice suggest that ECE-1 overexpression could be mediated by reactive oxygen species in an AP-1–dependent mechanism, in which some kinases such as PI3-kinase, Akt, erk1/2, and Jun Kinase could be involved. An increased activity of nicotinamide adenine dinucleotide phosphate oxidase seems to be the main source of reactive oxygen species. Conclusions— Mice lacking telomerase activity show hypertension as a result of an increase in plasma ET-1 levels, which is a consequence of ECE-1 overexpression. A direct link between telomerase activity and hypertension is reported.
Replica molding using polymeric materials: A practical step toward nanomanufacturing

Electrochemical synthesis of the hybrid PPy/PMol2: This was performed by multiple cyclic voltammograms using a Pt foil working electrode, a Agi AgCl reference electrode, and Pt coil as counter-electrode. Thus, 0.91 g of H3[M~IZ040] . xHzO (x = 17 as determined by TGA, FW = 2131.5) was dissolved in 50 mL of water and mixed with a solution of 0.70 mL of pyrrole in 50 mL of water. The mixture turned instantaneously from yellow to blue due to the partial oxidation of pyrrole by the phosphomolybdate. The complete oxidative polymerization was carried out by consecutive cyclic potential scans from +0.5 to -0.2 V (vs. AgiAgCl) at a scanning rate of 1 mVls. Chemical analyses revealed experimental values of 20.65 % C, 6.14% N, and 1.39 'L H. 44.61 Yo hilo.
Modulatory effects mediated by metabotropic glutamate receptor 5 on lateral geniculate nucleus relay cells

Glutamate is thought to be the excitatory neurotransmitter in the lateral geniculate nucleus (LGN) of the cat, mediating visual transmission from the retina via ionotropic receptors of both d,l‐α‐amino‐3‐hydroxy‐5‐α‐methyl‐4‐isoxazolepropionate and N‐methyl‐d‐aspartate subtypes. Moreover, glutamate also exerts an important modulatory influence on LGN cells, where metabotropic glutamate receptors (mGluRs) seem to play a crucial role. Here we show in anesthetized adult cats that iontophoretic application of the specific mGluR5 antagonist 2‐methyl‐6‐(phenylethynyl)‐pyridine (MPEP) produced two, distinctly different, effects on LGN neurons. Visual responses to flashing spots and drifting gratings were attenuated (decreased by an average of 59%) in 13 of 23 of the cells but augmented (increased by an average of 60%) in 10 of 23 of the cells. Further, in each case when the specific mGluR5 agonist (R,S)‐2‐chloro‐5‐hydroxyphenylglycine was applied, the effects obtained were the opposite to those of MPEP. Data obtained in a second group of experiments to determine a possible interaction between mGluR5 blockade by MPEP and glutamate ionotropic receptors show that, in the majority of neurons (11 of 15, 73%), the MPEP‐mediated effects seem to be independent of N‐methyl‐d‐aspartate and d,l‐α‐amino‐3‐hydroxy‐5‐α‐methyl‐4‐isoxazolepropionate receptor activity. Our results demonstrate a physiological role for mGluR5 in controlling retinal input and show, in vivo, a more intricate scenario than previously suggested, highlighting the complexity of metabotropic receptor interactions with excitatory and inhibitory elements in the thalamus.
Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis

To determine the genotypes of Toxoplasma gondii strains associated with human toxoplasmosis, we developed a sensitive approach for typing parasites grown from clinical samples by short-term in vitro culture. A newly described nested PCR assay was capable of amplifying genomic DNA from as few as five parasites in the presence of host tissues. Typing was based on DNA polymorphisms at the SAG2 locus, encoding tachyzoite surface antigen p22. Restriction fragment length polymorphisms in PCR-amplified SAG2 products were used to classify strains into one of the three major lineages of T. gondii. This approach was successfully used to determine the genotypes of 68 of 72 samples that had been previously isolated from patients with congenital, cerebral, and disseminated toxoplasmosis. Type II strains of T. gondii were found in a majority of the samples, accounting for 55 (81%) of the 68 toxoplasmosis cases. In contrast, type I and III strains were found in only 7 (10%) and 6 (9%) of the 68 cases, respectively. The results of this study support the previous finding that type II strains are most often associated with human toxoplasmosis. Nested PCR analysis at the SAG2 locus provides rapid assignment of T. gondii to a specific genotype that should be useful in analyzing a variety of clinical samples.
Obscured and unobscured AGN populations in a hard-X-ray selected sample of the XMDS survey

Aims. Our goal is to probe the populations of obscured and unobscured AGN investigating their optical-IR and X-ray properties as a function of X-ray flux, luminosity and redshift within a hard X-ray selected sample with wide multiwavelength coverage. Methods. We selected a sample of 136 X-ray sources detected at a significance of ≥3σ in the 2-10 keV band (F_(2-10) ≳ 10^(-14) erg cm^(-2) s^(-1)) in a ~1 deg^2 area in the XMM Medium Deep Survey (XMDS). The XMDS area is covered with optical photometry from the VVDS and CFHTLS surveys and infrared Spitzer data from the SWIRE survey. Based on the X-ray luminosity and X-ray to optical ratio, 132 sources are likely AGN, of which 122 have unambiguous optical - IR identification. The observed optical and IR spectral energy distributions of all identified sources are fitted with AGN/galaxy templates in order to classify them and compute photometric redshifts. X-ray spectral analysis is performed individually for sources with a sufficient number of counts and using a stacking technique for subsamples of sources at different flux levels. Hardness ratios are used to estimate X-ray absorption in individual weak sources. Results. 70% of the AGN are fitted by a type 2 AGN or a star forming galaxy template. We group them together in a single class of "optically obscured" AGN. These have "red" optical colors and in about 60% of cases show significant X-ray absorption (N_H > 10^(22) cm^(-2)). Sources with SEDs typical of type 1 AGN have "blue" optical colors and exhibit X-ray absorption in about 30% of cases. The stacked X-ray spectrum of obscured AGN is flatter than that of type 1 AGN and has an average spectral slope of Γ = 1.6. The subsample of objects fitted by a star forming galaxy template has an even harder stacked spectrum, with Γ ~ 1.2-1.3. The obscured fraction is larger at lower fluxes, lower redshifts and lower luminosities. X-ray absorption is less common than "optical" obscuration and its incidence is nearly constant with redshift and luminosity. This implies that at high luminosities X-ray absorption is not necessarily related to optical obscuration. The estimated surface densities of obscured, unobscured AGN and type 2 QSOs are respectively 138, 59 and 35 deg^(-2) at F > 10^(-14) erg cm^(-2) s^(-1).
Psychosocial influences: Critiques, findings, and research needs

Nongenetic factors have a major influence on psychopathology. Knowledge on specific psychosocial risk and protective mechanisms is more limited because of inadequate attention to measurement issues, person effects on the environment, and the possibility of genetic mediation. Nevertheless, a range of research strategies may be used to provide rigorous tests of causal hypotheses; these have shown the importance of environmentally mediated risks. Challenges for the future include greater use of such research strategies, improved measures of psychosocial risks that can be applied to large samples, investigation of origins of risks, identification of causes of time trends in levels of psychopathology, delineation of psychosocial effects on lifetime liability, understanding of environmental effects on the organism, appreciation of processes involved in developmental programming, and understanding of individual differences in susceptibility.
UDP-Glucuronosyltransferase 1A Compromises Intracellular Accumulation and Anti-Cancer Effect of Tanshinone IIA in Human Colon Cancer Cells

Background and Purpose NAD(P)H: quinone oxidoreductase 1 (NQO1) mediated quinone reduction and subsequent UDP-glucuronosyltransferases (UGTs) catalyzed glucuronidation is the dominant metabolic pathway of tanshinone IIA (TSA), a promising anti-cancer agent. UGTs are positively expressed in various tumor tissues and play an important role in the metabolic elimination of TSA. This study aims to explore the role of UGT1A in determining the intracellular accumulation and the resultant apoptotic effect of TSA. Experimental Approach We examined TSA intracellular accumulation and glucuronidation in HT29 (UGT1A positive) and HCT116 (UGT1A negative) human colon cancer cell lines. We also examined TSA-mediated reactive oxygen species (ROS) production, cytotoxicity and apoptotic effect in HT29 and HCT116 cells to investigate whether UGT1A levels are directly associated with TSA anti-cancer effect. UGT1A siRNA or propofol, a UGT1A9 competitive inhibitor, was used to inhibit UGT1A expression or UGT1A9 activity. Key Results Multiple UGT1A isoforms are positively expressed in HT29 but not in HCT116 cells. Cellular S9 fractions prepared from HT29 cells exhibit strong glucuronidation activity towards TSA, which can be inhibited by propofol or UGT1A siRNA interference. TSA intracellular accumulation in HT29 cells is much lower than that in HCT116 cells, which correlates with high expression levels of UGT1A in HT29 cells. Consistently, TSA induces less intracellular ROS, cytotoxicity, and apoptotic effect in HT29 cells than those in HCT116 cells. Pretreatment of HT29 cells with UGT1A siRNA or propofol can decrease TSA glucuronidation and simultaneously improve its intracellular accumulation, as well as enhance TSA anti-cancer effect. Conclusions and Implications UGT1A can compromise TSA cytotoxicity via reducing its intracellular exposure and switching the NQO1-triggered redox cycle to metabolic elimination. Our study may shed a light in understanding the cellular pharmacokinetic and molecular mechanism by which UGTs determine the chemotherapy effects of drugs that are UGTs’ substrates.
Simulating human mobility and information diffusion

Human spatial motions determine geographic social contacts that influence the way an information is spread on a population or a community. As mobility is a transverse dimension to social practices it is important to better understand its role. With the Eternal-Return model we propose, we simulate an artificial world populated by heterogeneous agents who differ in their mobility. We have chosen a multi-agent framework perspective for this simulation. We endow the agents with simple rules on how to move around the space and how to establish proximity-contacts. This allows to distinguish different kinds of mobile agents, from sedentary ones to travelers. To summarize the dynamics induced by mobility over time, we define the mobility-based Social Proximity Network as being the network of all distinct contacts between agents. Its properties give insight in the process of information spreading. We conduct simulations to understand how an information can be broadcast when agent-nodes are in motion.
Luteolin Nanoparticle in Chemoprevention: In Vitro and In Vivo Anticancer Activity

Cancer prevention (chemoprevention) by using naturally occurring dietary agents has gained immense interest because of the broad safety window of these compounds. However, many of these compounds are hydrophobic and poorly soluble in water. They frequently display low bioavailability, poor systemic delivery, and low efficacy. To circumvent this problem, we explored a novel approach toward chemoprevention using nanotechnology to deliver luteolin, a natural compound present in green vegetables. We formulated water-soluble polymer-encapsulated Nano-Luteolin from hydrophobic luteolin, and studied its anticancer activity against lung cancer and head and neck cancer. In vitro studies demonstrated that, like luteolin, Nano-Luteolin inhibited the growth of lung cancer cells (H292 cell line) and squamous cell carcinoma of head and neck (SCCHN) cells (Tu212 cell line). In Tu212 cells, the IC50 value of Nano-Luteolin was 4.13 μmol/L, and that of luteolin was 6.96 μmol/L. In H292 cells, the IC50 of luteolin was 15.56 μmol/L, and Nano-Luteolin was 14.96 μmol/L. In vivo studies using a tumor xenograft mouse model demonstrated that Nano-Luteolin has a significant inhibitory effect on the tumor growth of SCCHN in comparison to luteolin. Our results suggest that nanoparticle delivery of naturally occurring dietary agents like luteolin has many advantages and may have potential application in chemoprevention in clinical settings. Cancer Prev Res; 7(1); 65–73. ©2013 AACR.
Immunocryosurgery ‐ an effective combinational modality for Bowen's disease

Bowen's disease (BD) is widely treated with topical imiquimod or cryosurgery. The present single‐center retrospective study reports on the application of standardized immunocryosurgery (cryosurgery during ongoing topical imiquimod) for the treatment of BD. Daily imiquimod 5% cream was applied on BD lesion and a 5 mm rim around it in 5‐week treatment cycles; cryosurgery (liquid N2, open spray; 2 cycles, 15 second each) was performed at the end of the second week of each treatment cycle. Between 1/1/2009 and 31/12/2014 21 patients (mean age ± SD: 74.4 ± 8.0 years; 12 males) with 24 lesions (mean maximum diameter ± SD: 45.8 ± 50.9 mm; range: 9–200 mm) completed the protocol. The anatomic distribution of the lesions included face/scalp (Ν = 14), neck/trunk (Ν = 6), and extremities (Ν = 4). Twenty‐one out of twenty‐four lesions with diameter <80 mm cleared after one immunocryosurgery cycle, while the rest three tumors (with the largest diameters: 100, 180, 200 mm) required two treatment cycles for complete response (clearance rate: 100%). After a median follow‐up of 24 months (range: 6–60 months) the overall effectiveness was 91.7%: 22/24 lesions remained in sustained complete remission. With the exception of a variable degree of hypopigmentation, the cosmetic outcome was satisfactory even for extensive lesions. Immunocryosurgery, is feasible and highly efficacious minimally‐invasive treatment alternative for BD.