Miracle-dz/UniFAR-FTUs huggingface dataset:
Oral N-Acetyl-Cysteine Attenuates Loss of Dopaminergic Terminals in α-Synuclein Overexpressing Mice

Levels of glutathione are lower in the substantia nigra (SN) early in Parkinson's disease (PD) and this may contribute to mitochondrial dysfunction and oxidative stress. Oxidative stress may increase the accumulation of toxic forms of α-synuclein (SNCA). We hypothesized that supplementation with n-acetylcysteine (NAC), a source of cysteine – the limiting amino acid in glutathione synthesis, would protect against α-synuclein toxicity. Transgenic mice overexpressing wild-type human α-synuclein drank water supplemented with NAC or control water supplemented with alanine from ages 6 weeks to 1 year. NAC increased SN levels of glutathione within 5–7 weeks of treatment; however, this increase was not sustained at 1 year. Despite the transient nature of the impact of NAC on brain glutathione, the loss of dopaminergic terminals at 1 year associated with SNCA overexpression was significantly attenuated by NAC supplementation, as measured by immunoreactivity for tyrosine hydroxylase in the striatum (p = 0.007; unpaired, two-tailed t-test), with a similar but nonsignificant trend for dopamine transporter (DAT) immunoreactivity. NAC significantly decreased the levels of human SNCA in the brains of PDGFb-SNCA transgenic mice compared to alanine treated transgenics. This was associated with a decrease in nuclear NFκB localization and an increase in cytoplasmic localization of NFκB in the NAC-treated transgenics. Overall, these results indicate that oral NAC supplementation decreases SNCA levels in brain and partially protects against loss of dopaminergic terminals associated with overexpression of α-synuclein in this model.
Comparison of methods for quantifying dental wear caused by erosion and abrasion

Various methods have been applied to evaluate the effect of erosion and abrasion. So, the aim of this study was to check the applicability of stylus profilometry (SP), surface hardness (SH) and focus‐variation 3D microscopy (FVM) to the analysis of human enamel and dentin subjected to erosion/abrasion. The samples were randomly allocated into four groups (n = 10): G1‐enamel/erosion, G2‐enamel/erosion plus abrasion, G3‐dentin/erosion, and G4‐dentin/erosion plus abrasion. The specimens were selected by their surface hardness, and they were subjected to cycles of demineralization (Coca‐Cola®‐60 s) and remineralization (artificial saliva‐60 min). For groups G2 and G4, the remineralization procedures were followed by toothbrushing (150 strokes). The above cycle was repeated 3×/day during 5 days. The samples were assessed using SH, SP, and FVM. For each substrate, the groups were compared using an unpaired t‐test, and Pearson correlation coefficients were calculated (α = 5%). For enamel, both profilometry technique showed greater surface loss when the erosion and abrasion processes were combined (P <0.05). The correlation analysis did not reveal any relationships among SH, SP, and FVM to G2 and G4. There were significant correlation coefficients (–0.70 and –0.67) for the comparisons between the FVM and SH methods in enamel and dentin, respectively, in G1 and G3. Choosing the ideal technique for the analysis of erosion depends on the type of dental substrate. SP was not sufficiently sensitive to measure the effects on dentin of erosion or erosion/abrasion. However, SP, FVM and SH were adequate for the detection of tissue loss and demineralization in enamel. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.
The role of serotonin 5-HT2A receptors in memory and cognition

Serotonin 5-HT2A receptors (5-HT2ARs) are widely distributed in the central nervous system, especially in brain region essential for learning and cognition. In addition to endogenous 5-HT, several hallucinogens, antipsychotics, and antidepressants function by targeting 5-HT2ARs. Preclinical studies show that 5-HT2AR antagonists have antipsychotic and antidepressant properties, whereas agonist ligands possess cognition-enhancing and hallucinogenic properties. Abnormal 5-HT2AR activity is associated with a number of psychiatric disorders and conditions, including depression, schizophrenia, and drug addiction. In addition to its traditional activity as a G protein-coupled receptor (GPCR), recent studies have defined novel operations of 5-HT2ARs. Here we review progress in the (1) receptor anatomy and biology: distribution, signaling, polymerization and allosteric modulation; and (2) receptor functions: learning and memory, hallucination and spatial cognition, and mental disorders. Based on the recent progress in basic research on the 5-HT2AR, it appears that post-training 5-HT2AR activation enhances non-spatial memory consolidation, while pre-training 5-HT2AR activation facilitates fear extinction. Further, the potential influence that 5-HT2AR-elicited visual hallucinations may have on visual cue (i.e., landmark) guided spatial cognition is discussed. We conclude that the development of selective 5-HT2AR modulators to target distinct signaling pathways and neural circuits represents a new possibility for treating emotional, neuropsychiatric, and neurodegenerative disorders.
In the blink of an eye: combining head motion and eye blink frequency for activity recognition with Google Glass

We demonstrate how information about eye blink frequency and head motion patterns derived from Google Glass sensors can be used to distinguish different types of high level activities. While it is well known that eye blink frequency is correlated with user activity, our aim is to show that (1) eye blink frequency data from an unobtrusive, commercial platform which is not a dedicated eye tracker is good enough to be useful and (2) that adding head motion patterns information significantly improves the recognition rates. The method is evaluated on a data set from an experiment containing five activity classes (reading, talking, watching TV, mathematical problem solving, and sawing) of eight participants showing 67% recognition accuracy for eye blinking only and 82% when extended with head motion patterns.
Translational Suppression of Atrophic Regulators by MicroRNA-23a Integrates Resistance to Skeletal Muscle Atrophy*

Muscle atrophy is caused by accelerated protein degradation and occurs in many pathological states. Two muscle-specific ubiquitin ligases, MAFbx/atrogin-1 and muscle RING-finger 1 (MuRF1), are prominently induced during muscle atrophy and mediate atrophy-associated protein degradation. Blocking the expression of these two ubiquitin ligases provides protection against muscle atrophy. Here we report that miR-23a suppresses the translation of both MAFbx/atrogin-1 and MuRF1 in a 3′-UTR-dependent manner. Ectopic expression of miR-23a is sufficient to protect muscles from atrophy in vitro and in vivo. Furthermore, miR-23a transgenic mice showed resistance against glucocorticoid-induced skeletal muscle atrophy. These data suggest that suppression of multiple regulators by a single miRNA can have significant consequences in adult tissues.
Nonexercise energy expenditure and physical activity in the Midwest Exercise Trial 2.

PURPOSE This study aimed to examine compensatory changes in nonexercise energy expenditure (NEEx) and nonexercise physical activity (NEPA) in response to an aerobic exercise training program. METHODS Ninety-two overweight/obese (body mass index, 25-39.9 kg·m) sedentary young adults (18-30 yr) completed a 10-month randomized clinical efficacy trial of aerobic exercise 5 d·wk at either 400 kcal per session (n = 37), 600 kcal per session (n = 37), or control (n = 18). Total daily energy expenditure (TDEE) and resting metabolic rate (RMR) were measured at months 0 and 10. NEPA was measured by an accelerometer at months 0, 3.5, 7, and 10. NEEx was calculated by the following formula: [(total daily energy expenditure × 0.9) - RMR] - net EEEx (EEEx-RMR). Mixed modeling was used to examine differences between groups (group effect), within groups (time effect), and group-time interaction for NEEx and NEPA. RESULTS Within the exercise groups, there were no significant effects (all P > 0.05) of group, time, or group-time interaction for NEPA. In addition, there were no significant within- or between-group differences for change in NEEx. However, activity counts per minute were significantly higher (P < 0.001) in the 600-kcal-per-session group (346 ± 141 min·d) versus controls (290 ± 106 min·d) at month 7 and significantly higher (P < 0.001) in both the 600-kcal-per-session (345 ± 163 min·d) and 400-kcal-per-session groups (317 ± 146 min·d) versus controls (277 ± 116 min·d) at 10 months. CONCLUSIONS A 10-month aerobic exercise training program in previously sedentary, overweight and obese young adults was not associated with compensatory decreases in NEEx or NEPA. Results suggest that overweight and obese individuals do not become less physically active or spend more time in sedentary pursuits in response to exercise.
Physical and Psychosocial Effects of Wii Video Game Use among Older Women

This study investigated the physical and psychosocial effect of exergaming in community dwelling older adult women. In a pilot study consisting of a six-week baseline period and a six-week intervention period, participants (N = 11, mean age = 73.5 years, SD = 9.0) played Nintendo Wii Sports twice weekly. We measured full body movements using accelerometers, and assessed psychosocial effects through end-of-study focus group meetings. There were large self-reported psychological effects related to positive changes in self perception. The game-play deepened social connections within the group and provided a basis for shared experiences with younger aged family members. Physically, the game-play showed significantly higher maximum energy expenditure (t = -4.52, p < 0.05) than baseline, but no significant difference in overall energy expenditure. Findings from the quantitative data showed that Wii-play did not have substantial physical effects; nevertheless, qualitative data revealed that the participants perceived an improved sense of physical, social and psychological wellbeing.
Multicast time maximization in energy constrained wireless networks

We consider the problem of maximizing the lifetime of a given multicast connection in a wireless network of energy-constrained (e.g. battery-operated) nodes, by choosing ideal transmission power levels for the nodes relaying the connection. We distinguish between two basic operating modes: In a static assignment, the power levels of the nodes are set at the beginning and remain unchanged until the nodes are depleted of energy. In a dynamic assignment, the powers can be adjusted during operation.We show that lifetime-maximizing static power assignments can be found in polynomial time, whereas for dynamic assignments, a quantized-time version of the problem is NP-hard. We then study the approximability of the quantized dynamic case and conclude that no polynomial time approximation scheme (PTAS) exists for the problem unless Ptime = NP. Finally, by considering two approximation heuristics for the dynamic case, we show experimentally that the lifetime of a dynamically maintained multicast connection can be made several times longer than what can be achieved by the best possible static assignment.
Relative Risk Perception for Terrorism: Implications for Preparedness and Risk Communication

Terrorism presents a significant risk that is often approached at public policy, infrastructure, or emergency management level. Public perceptions of the likelihood of terrorist events, and how this may relate to individual preparedness, are not always extensively examined. The tendency to think that negative events are less likely to happen to oneself than to the average person is known as optimism bias. Optimism bias is relevant to perceptions of terrorism, because it is thought to be related to a reduction in precaution use. Using an online survey of 164 participants, this study aimed to determine whether Sydney residents thought they had a lower likelihood of experiencing terrorist events than other Australians. Significant optimism bias was observed for witnessing terrorist events, but not for personally experiencing terrorist events. In addition, Sydney residents tended to think that terrorist attacks were more likely to occur in Sydney than another major Australian city in the next five years. At the same time, household and workplace preparedness for terrorism was quite low, as was awareness of emergency strategies in the central business district. Perceptions of high likelihood of terrorism happening in one's own city, yet low preparedness present a challenge for risk communication and emergency management strategies. The diversity of possible terrorist targets, and the simple plans that can moderate the effects of a disaster may need to be emphasized in future anti‐terrorism initiatives.
Concentration-dependent Effects of D-Methylphenidate on Frontal Cortex and Spinal Cord Networks in vitro

Spontaneously active frontal cortex and spinal cord networks grown on microelectrode arrays were used to study effects of D-methylphenidate. These central nervous system tissues have relatively low concentrations of dopaminergic and noradrenergic neurons compared to the richly populated loci, yet exhibit similar neurophysiological responses to methylphenidate. The spontaneous spike activity of both tissues was inhibited in a concentration-dependent manner by serial additions of 1-500 µM methylphenidate. Methylphenidate is non-toxic as spike inhibition was recovered following washes. The average concentrations for 50% spike rate inhibition (IC50 ± SD) were 118 ± 52 (n= 6) and 57 ± 43 (n = 11) for frontal cortex and spinal cord networks, respectively. A 3 hour exposure of a network to 1 mM methylphenidate was nontoxic. The effective concentrations described in this study are within the therapeutic dosage range. Therefore, the platform may be used for further investigations of drug mechanisms.
Are difficulties in mentalizing associated with severity of Antisocial Personality Disorder

Part 1 of the thesis reviews the literature on the measurement of mentalization in adult clinical populations. As mentalization is a broad multi-faceted term, the search incorporates the related concepts of Theory of Mind (ToM) and Emotional Intelligence (EI) as these have been widely operationalised. The review presents a framework for different types of measures, including performance-based tasks and self-report questionnaires, and considers their relative psychometric strengths. It finds an absence of any one measure that covers the breadth of the mentalization construct, however, a set of recommendations are made for an optimal approach using currently available tools. Part 2 presents an empirical study of the relationship between mentalizing capability and severity of Antisocial Personality Disorder (ASPD) in an offender sample. The results show that some specific mentalizing measures were able to modestly predict severity of ASPD. These were the ability to take the perspective of another person, the ability to read mental states from the ‘eyes’ and a general inability to mentalize. These findings suggest that a greater understanding of mentalizing capacities in people with ASPD may support improved risk assessment and clinical treatments. The study’s limitations are considered and its implications for further research and practice. Part 3 presents a critical appraisal of the process of undertaking this research. It describes some of the challenges to joint working across the NHS and the Criminal Justice System. It considers how the use of psychometric assessment can be improved in an ASPD/offender population. It builds on the literature review to recommend how the operationalisation of mentalization can be further enhanced.
Drug use, sex work and the risk environment in Dublin.

Action 98 of the National Drugs Strategy 2001-2008 required the NACD to carry out research on drug misuse among at-risk groups, including prostitutes/sex workers. In response, the NACD undertook this study to explore female and male problematic drug-using sex workers' lived experience of risk, and how the risk environment can mediate the individual’s capacity to reduce the risk of harm. The concept of the risk environment was broadly defined as comprising risk factors that are external to the individual. Thus, the 'environment' encompasses not only the physical space within which drug-using sex workers live and work, but also the social, economic and policy environment.
Automatic Tablature Transcription of Electric Guitar Recordings by Estimation of Score- and Instrument-Related Parameters

In this paper we present a novel algorithm for automatic analysis, transcription, and parameter extraction from isolated polyphonic guitar recordings. In addition to general score-related information such as note onset, duration, and pitch, instrumentspecific information such as the plucked string, the applied plucking and expression styles are retrieved automatically. For this purpose, we adapted several state-of-the-art approaches for onset and offset detection, multipitch estimation, string estimation, feature extraction, and multi-class classification. Furthermore we investigated a robust partial tracking algorithm with respect to inharmonicity, an extensive extraction of novel and known audio features as well as the exploitation of instrument-based knowledge in the form of plausability filtering to obtain more reliable prediction. Our system achieved very high accuracy values of 98 % for onset and offset detection as well as multipitch estimation. For the instrument-related parameters, the proposed algorithm also showed very good performance with accuracy values of 82 % for the string number, 93 % for the plucking style, and 83 % for the expression style. Index Terms - playing techniques, plucking style, expression style, multiple fundamental frequency estimation, string classification, fretboard position, fingering, electric guitar, inharmonicity coefficient, tablature
Brain-computer interface-based robotic end effector system for wrist and hand rehabilitation: results of a three-armed randomized controlled trial for chronic stroke

The objective of this study was to investigate the efficacy of an Electroencephalography (EEG)-based Motor Imagery (MI) Brain-Computer Interface (BCI) coupled with a Haptic Knob (HK) robot for arm rehabilitation in stroke patients. In this three-arm, single-blind, randomized controlled trial; 21 chronic hemiplegic stroke patients (Fugl-Meyer Motor Assessment (FMMA) score 10–50), recruited after pre-screening for MI BCI ability, were randomly allocated to BCI-HK, HK or Standard Arm Therapy (SAT) groups. All groups received 18 sessions of intervention over 6 weeks, 3 sessions per week, 90 min per session. The BCI-HK group received 1 h of BCI coupled with HK intervention, and the HK group received 1 h of HK intervention per session. Both BCI-HK and HK groups received 120 trials of robot-assisted hand grasping and knob manipulation followed by 30 min of therapist-assisted arm mobilization. The SAT group received 1.5 h of therapist-assisted arm mobilization and forearm pronation-supination movements incorporating wrist control and grasp-release functions. In all, 14 males, 7 females, mean age 54.2 years, mean stroke duration 385.1 days, with baseline FMMA score 27.0 were recruited. The primary outcome measure was upper extremity FMMA scores measured mid-intervention at week 3, end-intervention at week 6, and follow-up at weeks 12 and 24. Seven, 8 and 7 subjects underwent BCI-HK, HK and SAT interventions respectively. FMMA score improved in all groups, but no intergroup differences were found at any time points. Significantly larger motor gains were observed in the BCI-HK group compared to the SAT group at weeks 3, 12, and 24, but motor gains in the HK group did not differ from the SAT group at any time point. In conclusion, BCI-HK is effective, safe, and may have the potential for enhancing motor recovery in chronic stroke when combined with therapist-assisted arm mobilization.
Extracellular matrix as target for antitumor therapy

The aim of the present review is to survey the accumulated knowledge on the extracellular matrix (ECM) of tumors referring to its putative utility as therapeutic target. Following the traditional observation on the extensive morphological alteration in the tumor-affected tissue, the well-documented aberrant cellular regulation indicated that ECM components have an active role in tumor progression. However, due to the diverse functions and variable expression of proteoglycans, matrix proteins, and integrins, it is rather difficult to identify a comprehensive therapeutic target among ECM components. At present, the elevated level of heparanase and the prominent expression of αvβ5 integrin are considered as promising therapeutic targets. The inhibition of glycosaminoglycan offers another promising approach in the treatment of those tumors which are stimulated by proteoglycans. It can be ascertained that a selective ECM inhibitor would be a great asset to control metastasis driven by ECM-mediated signaling.
Sustained attention and prediction: distinct brain maturation trajectories during adolescence

Adolescence is a key period for frontal cortex maturation necessary for the development of cognitive ability. Sustained attention and prediction are cognitive functions critical for optimizing sensory processing, and essential to efficiently adapt behaviors in an ever-changing world. The aim of the current study was to investigate the brain developmental trajectories of attentive and predictive processing through adolescence. We recorded EEG in 36 participants from the age of 12–24 years (three age groups: 12–14, 14–17, 18–24 years) to target development during early and late adolescence, and early adulthood. We chose a visual target detection task which loaded upon sustained attention, and we manipulated target predictability. Continued maturation of sustained attention after age 12 was evidenced by improved performance (hits, false alarms (FAs) and sensitivity) in a detection task, associated with a frontal shift in the scalp topographies of the Contingent Negative Variation (CNV) and P3 responses, with increasing age. No effect of age was observed on predictive processing, with all ages showing similar benefits in reaction time, increases in P3 amplitude (indexing predictive value encoding and memorization), increases in CNV amplitude (corresponding to prediction implementation) and reduction in target-P3 latency (reflecting successful prediction building and use), with increased predictive content. This suggests that adolescents extracted and used predictive information to generate predictions as well as adults. The present results show that predictive and attentive processing follow distinct brain developmental trajectories: prediction abilities seem mature by the age of 12 and sustained attention continues to improve after 12-years of age and is associated with maturational changes in the frontal cortices.