Miracle-dz/UniFAR-FTUs huggingface dataset:
SCUTICOCILIATID CILIATE OUTBREAK IN AUSTRALIAN POT-BELLIED SEAHORSE, HIPPOCAMPUS ABDOMINALIS (LESSON, 1827): CLINICAL SIGNS, HISTOPATHOLOGIC FINDINGS, AND TREATMENT WITH METRONIDAZOLE

Abstract: A severe outbreak of scuticociliatosis occurred in Australian pot-bellied seahorse, Hippocampus abdominalis (Lesson, 1872), kept at the Vancouver Aquarium Marine Science Centre (Vancouver, British Columbia, Canada). Clinical signs included anorexia, lethargy, irregular respiration, and death. Cytology and histopathology revealed a high number of histophagous ciliated protozoa within the tissues. The parasite, identified as Philasterides dicentrarchi, was observed in several internal organs that appeared edematous and hemorrhagic upon postmortem examination. Severe histopathologic lesions were reported in particular in the ovary, the kidney, and the intestine. This infection was successfully treated with metronidazole via bath therapy. No further evidence of this parasite was found in the treated fish.
Effect of magnesium addition on evolution of inclusions in Mn–Si–Al deoxidised molten steels

Abstract To reveal the effects of magnesium on the evolution of inclusions in Mn–Si–Al deoxidised steels, both thermodynamic calculations and deoxidised experiments were carried out in the present work. The samples extracted from the melts were polished and analysed by optical microscope and scanning electron microscopy (SEM). The results showed that magnesium can control the size of oxide inclusions in Mn–Si–Al deoxidised melts well. Superfluous MgO raise the melting point of the complex inclusions, and then worsen the steel castability. Therefore, the amount of Mg addition should be under control. The recommend value of [Mg] in present work is 1–3 ppm, based on thermodynamic calculations and experiments. All inclusions observed in the sample were nearly spherical. The evolution mechanisms of inclusions were discussed comprehensively, and proper models for the formation of oxide inclusions were set up. In practice, to achieve the full liquid inclusions in molten steel, the addition amount of magnesium and aluminium should be considered simultaneously.
Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken

Segmented double-stranded RNA (dsRNA) viruses were identified by polyacrylamide gel electrophoresis (PAGE) technique in fecal samples from broiler chicken. A total of 378 fecal samples from 1-7 weeks old chickens were analyzed. dsRNA with migration profile characteristic of avian rotavirus (AvRV), reovirus (ARV) or picobirnavirus (PBV) was identified in 32 (8.5%), 7 (1.8%) and 13 (3.4%) samples, respectively. AvRV and ARV occurred more frequently in chickens up to 1 month old and were related with enteritis signs. Considering only fecal samples of chickens with diarrhea, the AvRV was detected in 37.8% (14/37) and the ARV in 13.5% (5/37) of analyzed samples. AvRV was identified in only 1.5% (4/274) and ARV was not detected in normal feces collected from assymptomatic chickens (controls). PBV dsRNA was detected in broiler chickens from two to seven weeks old, more frequently in feces with pasty consistency. The AvRV showed great electrophoretic profile variability in the dsRNA segments and nine different electropherotypes were identified. Variation in genome pattern was not observed in either ARV or PBV.
Melioidotic osteomyelitis in northern Australia.

BACKGROUND Osteomyelitis is a well-recognized manifestation of melioidosis, a significant disease that is endemic in South-East Asia and northern Australia. Diagnosis requires a high degree of clinical suspicion and is dependent on microbiological confirmation; important clues are travel to or residence in an endemic area. Infection often but not always occurs in well-recognized risk groups, especially diabetics and alcoholics. Subacute presentations often mimic other disease processes and patients may not always be clinically septic. Treatment requires surgical drainage in combination with multiple antibiotic therapy, including at least 2 weeks of intravenous ceftazidime and oral therapy continuing for 3-6 months. Non-compliance usually results in relapse. Due to the potential long latency of the disease and the possibility of reactivation, follow-up should probably be life-long. METHODS A prospective study of 115 patients presenting with melioidosis between 1989 and 1995 was undertaken. RESULTS Four patients were found to have osteomyelitis. CONCLUSION It is important to be aware of this condition so that early treatment can be initiated.
Cryptosporidium spp. in calves and cows from organic and conventional dairy herds

SUMMARY A cohort study was performed to investigate cryptosporidial prevalence and species distribution in 13 organic and 13 conventional dairy herds. Faecal samples were collected from 221 calves and 259 cows. Management routines were recorded at farm inspection and through a questionnaire. Samples were concentrated using sodium chloride flotation and cryptosporidial oocysts were detected by epifluorescence microscopy. Molecular analysis was used to determine species and subtypes. A multivariable model for factors associated with calves being Cryptosporidium spp. positive was built. Cryptosporidium spp. -positive animals were identified in all herds. Prevalences were similar in organic and conventional calves (44·7% vs. 52·3%), as well as in cows (3·1% vs. 3·8%), P > 0·05. Cryptosporidium bovis, C. ryanae and C. parvum were identified. C. ryanae was identified in a calf younger than the described prepatent period. The multivariable model included four significant variables; calf age, cleanliness of bedding, cleaning routines for group pens and farmers' attitudes towards biosecurity.
Linkage maps from multiple genetic crosses and loci linked to growth-related virulent phenotype in Plasmodium yoelii

Plasmodium yoelii is an excellent model for studying malaria pathogenesis that is often intractable to investigate using human parasites; however, genetic studies of the parasite have been hindered by lack of genome-wide linkage resources. Here, we performed 14 genetic crosses between three pairs of P. yoelii clones/subspecies, isolated 75 independent recombinant progeny from the crosses, and constructed a high-resolution linkage map for this parasite. Microsatellite genotypes from the progeny formed 14 linkage groups belonging to the 14 parasite chromosomes, allowing assignment of sequence contigs to chromosomes. Growth-related virulent phenotypes from 25 progeny of one of the crosses were significantly associated with a major locus on chromosome 13 and with two secondary loci on chromosomes 7 and 10. The chromosome 10 and 13 loci are both linked to day 5 parasitemia, and their effects on parasite growth rate are independent but additive. The locus on chromosome 7 is associated with day 10 parasitemia. The chromosome 13 locus spans ∼220 kb of DNA containing 51 predicted genes, including the P. yoelii erythrocyte binding ligand, in which a C741Y substitution in the R6 domain is implicated in the change of growth rate. Similarly, the chromosome 10 locus spans ∼234 kb with 71 candidate genes, containing a member of the 235-kDa rhoptry proteins (Py235) that can bind to the erythrocyte surface membrane. Atypical virulent phenotypes among the progeny were also observed. This study provides critical tools and information for genetic investigations of virulence and biology of P. yoelii.
Differential Recognition of Surface Proteins in Streptococcus pyogenes by Two Sortase Gene Homologs

ABSTRACT The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous to Staphylococcus aureus sortase, which covalently links proteins containing an LPXTG motif to the cell wall. The GAS srtA mutant was defective in anchoring the LPXTG-containing proteins M6, protein F, ScpA, and GRAB to the cell surface. This phenotype was complemented when a wild-type srtA gene was provided in trans. The surface localization of T6, however, was unaffected by the srtA mutation. The M1 genome sequence contains a second open reading frame with a motif characteristic of sortase proteins. Inactivation of this gene (designated srtB) in strain JRS4 affected the surface localization of T6 but not M6, protein F, ScpA, or GRAB. This phenotype was complemented by srtB in trans. An srtA probe hybridized with DNA from all GAS strains tested (M types 1, 3, 4, 5, 6, 18, 22, and 50 and nontypeable strain 64/14) and from streptococcal groups C and G, while srtB hybridized with DNA from only a few GAS strains. We conclude that srtA and srtB encode sortase enzymes required for anchoring different subsets of proteins to the cell wall. It seems likely that the multiple sortase homologs in the genomes of other gram-positive bacteria have a similar substrate-specific role.
Radiation-Induced Esophagitis is Mitigated by Soy Isoflavones

Introduction Lung cancer patients receiving radiotherapy present with acute esophagitis and chronic fibrosis, as a result of radiation injury to esophageal tissues. We have shown that soy isoflavones alleviate pneumonitis and fibrosis caused by radiation toxicity to normal lung. The effect of soy isoflavones on esophagitis histopathological changes induced by radiation was investigated. Methods C57BL/6 mice were treated with 10 Gy or 25 Gy single thoracic irradiation and soy isoflavones for up to 16 weeks. Damage to esophageal tissues was assessed by hematoxylin–eosin, Masson’s Trichrome and Ki-67 staining at 1, 4, 10, and 16 weeks after radiation. The effects on smooth muscle cells and leukocyte infiltration were determined by immunohistochemistry using anti-αSMA and anti-CD45, respectively. Results Radiation caused thickening of esophageal tissue layers that was significantly reduced by soy isoflavones. Major radiation alterations included hypertrophy of basal cells in mucosal epithelium and damage to smooth muscle cells in muscularis mucosae as well as disruption of collagen fibers in lamina propria connective tissue with leukocyte infiltration. These effects were observed as early as 1 week after radiation and were more pronounced with a higher dose of 25 Gy. Soy isoflavones limited the extent of tissue damage induced by radiation both at 10 and 25 Gy. Conclusion Soy isoflavones have a radioprotective effect on the esophagus, mitigating the early and late effects of radiation injury in several esophagus tissue layers. Soy could be administered with radiotherapy to decrease the incidence and severity of esophagitis in lung cancer patients receiving thoracic radiation therapy.
The Utility of Using the Bispectral Index—Vista for Detecting Cross‐Clamping Decline in Cerebral Blood Flow Velocity

BACKGROUND Patients undergoing carotid endarterectomy for extracranial internal carotid artery stenosis are at risk of cerebral ischemia/hypoperfusion. Criterion recommended by European and American committees to determine whether to place a shunt consisted of a decline in transcranial Doppler ultrasonography—measured middle cerebral artery blood flow velocity (MCBFV) to < 30% to 40% of intraoperative preclamp value. OBJECTIVE To assess the discriminative power of the bispectral index (BIS)—Vista monitor for detecting a 40% decline in MCBFV with cross-clamping. METHODS In 20 patients undergoing carotid endarterectomy under remifentanil/propofol anesthesia, BIS—Vista data, MCBFV, and pulsatility index from bilaterally mounted BIS—Vista and transcranial Doppler monitors were continuously recorded. RESULTS Coefficient of determination revealed good correlation (r2 = 0.763) between ipsilateral BIS—Vista and MCBFV after cross-clamping. BIS—Vista exhibited a high discriminative power of 0.850 (95% confidence interval, 0.455–0.966) area under the receiver-operating characteristic curve in detecting an ipsilateral 40% MCBFV decline. Two-way analysis of variance (location by time) suggests that BIS—Vista exhibited a global decline; ie, both BIS—Vistas declined when 1 carotid on either side was clamped because there was no significant interhemispheric difference (P = .112) in mean BIS—Vista values over time. CONCLUSION Although we demonstrated good correlation and high discriminative power of the BIS—Vista monitor in depicting a MCBFV decline that could serve as indicator of decline in cerebral activity, BIS—Vista cannot be considered a reliable indicator of cerebral ischemia/hypoperfusion that could replace transcranial Doppler monitoring to determine whether a shunt is to be placed.
Quality Control for Crowdsourced Enumeration Tasks

Quality control is one of the central issues in crowdsourcing research. In this paper, we consider a quality control problem of crowdsourced enumeration tasks that request workers to enumerate possible answers as many as possible. Since workers neither necessarily provide correct answers nor provide exactly the same answers even if the answers indicate the same idea, we propose a two-stage quality control method consisting of the answer clustering stage and the reliability estimation stage.
Typeface personality traits and their design characteristics

Most research on fonts is related to legibility, readability and recognition. There are only a few studies on typefaces and their potential personality traits. In this paper, we focus on the visual expression of typefaces and their design characteristics. The relationship between typefaces and their personality traits is investigated. By using statistical analyses on data collected from participants who filled out a survey, the correlation between fonts and personality traits is explored. Fonts used within this study are grouped according to their personality traits. The typical typographical and aesthetic characteristics of typefaces in these groups are examined in detail.
Antithrombotic Treatment Following Intracerebral Hemorrhage in Patients With and Without Atrial Fibrillation

Background and Purpose— Patients who survive intracerebral hemorrhage (ICH) often have compelling indications for anticoagulant and antiplatelet medication. This nationwide observational study aimed to determine the extent and predictors of antithrombotic treatment after ICH in Sweden. Methods— Patients with a first-ever ICH in the Swedish Stroke Register (Riksstroke) 2005 to 2012 who survived hospital discharge were included. Riksstroke data were individually linked with other national registers to determine comorbid conditions and dispensed prescriptions of antithrombotic agents. Results— Among the 2777 patients with atrial fibrillation (AF), the proportion with a dispensed prescription of antithrombotic agents was 8.5% (anticoagulants) and 36.6% (antiplatelet agents) within 6 months and 11.1% (anticoagulants) and 43.6% (antiplatelet agents) within 1 year. Among the 11 268 patients without AF, the corresponding figures were 1.6% (anticoagulants) and 13.8% (antiplatelet agents) within 6 months and 2.0% (anticoagulants) and 17.5% (antiplatelet agents) within 1 year. In patients with AF, predictors of anticoagulant treatment were less severe ICH, younger age, previous anticoagulation, valvular disease, and previous ischemic stroke. High CHA2DS2-VASc (congestive heart failure, hypertension, age, diabetes mellitus, stroke [doubled], vascular disease, age, and sex category [female]) scores did not correlate with anticoagulant treatment. There was a positive correlation between high CHA2DS2-VASc and HAS-BLED (hypertension, abnormal renal/liver function, stroke, bleeding history or predisposition, labile international normalized ratio, elderly, drugs/alcohol) scores (rs=0.590, P<0.001). Conclusions— In majority of patients who receive antithrombotic agents, treatment is initiated within 6 months of ICH. Still, many patients with compelling indications for antithrombotic treatment are not prescribed antithrombotic agents. Factors other than high risk of embolic stroke by CHA2DS2-VASc in ICH survivors with concurrent AF are used to guide the anticoagulant treatment decision in Swedish clinical practice.
The Behavioral Relevance of Cortical Neural Ensemble Responses Emerges Suddenly

Whereas many laboratory-studied decisions involve a highly trained animal identifying an ambiguous stimulus, many naturalistic decisions do not. Consumption decisions, for instance, involve determining whether to eject or consume an already identified stimulus in the mouth and are decisions that can be made without training. By standard analyses, rodent cortical single-neuron taste responses come to predict such consumption decisions across the 500 ms preceding the consumption or rejection itself; decision-related firing emerges well after stimulus identification. Analyzing single-trial ensemble activity using hidden Markov models, we show these decision-related cortical responses to be part of a reliable sequence of states (each defined by the firing rates within the ensemble) separated by brief state-to-state transitions, the latencies of which vary widely between trials. When we aligned data to the onset of the (late-appearing) state that dominates during the time period in which single-neuron firing is correlated to taste palatability, the apparent ramp in stimulus-aligned choice-related firing was shown to be a much more precipitous coherent jump. This jump in choice-related firing resembled a step function more than it did the output of a standard (ramping) decision-making model, and provided a robust prediction of decision latency in single trials. Together, these results demonstrate that activity related to naturalistic consumption decisions emerges nearly instantaneously in cortical ensembles. SIGNIFICANCE STATEMENT This paper provides a description of how the brain makes evaluative decisions. The majority of work on the neurobiology of decision making deals with “what is it? ” decisions; out of this work has emerged a model whereby neurons accumulate information about the stimulus in the form of slowly increasing firing rates and reach a decision when those firing rates reach a threshold. Here, we study a different kind of more naturalistic decision—a decision to evaluate “what shall I do with it? ” after the identity of a taste in the mouth has been identified—and show that this decision is not made through the gradual increasing of stimulus-related firing, but rather that this decision appears to be made in a sudden moment of “insight. ”
FATIGUE TESTS OF REINFORCED CONCRETE BEAMS STRENGTHENED USING CARBON FIBER-REINFORCED POLYMER COMPOSITES

The use of carbon fiber-reinforced polymer (CFRP) composites has been identified as a viable technique for use in strengthening deteriorating or structurally deficient reinforced concrete bridges. This article presents the results of a study that consisted of repeated-load fatigue testing 10 reinforced concrete beams strengthened using two different types of externally-bonded CFRP composites. The results indicate that the bond between the composite laminates and surface of the concrete can degrade at load amplitudes corresponding to extreme load conditions for a bridge. The stress range applied to the reinforcing steel and maximum stress applied to the composite laminates were the controlling parameters that limited the fatigue life of the specimens under study. The authors conclude that an upper limit on stresses generated along the composite-concrete interface may have to be set during design to avoid premature debonding after a limited number of load cycles.
Perceptual evaluation of space in virtual environments

Floor plan designs and their spatial analysis are typically constrained to blueprints and 2D projections of 3D models. Computing appropriate spatial measures from such representations provides a standard way of quantifying important aspects of the design. We wish to investigate whether a person's perceptual exploration of a space would agree with such spatial measures, that is, whether a person can roughly infer such measures by exploring a space. We perform two studies, one involving novices and the other experts. First, we conduct a perceptual study to discover whether a novice user's perception of spatial measures depends on the mode used to explore the space. Our analysis considers three spatial measures, grounded in Space-Syntax, that characterize key aspects of a design such as visibility, accessibility, and organization. We compare three modes of exploration: 2D blueprints, first-person view in a 3D simulation, and a 3D virtual reality simulation with teleportation. A correlation analysis between the users' perceptual ratings and the spatial measures, indicates that virtual reality is the most effective of the three methods, while 2D blueprints and 3D first-person exploration often fail entirely to convey the spatial measures. In the second study, experts are asked to evaluate and rank the design blueprints for each measure. The expert observations are in strong agreement with the spatial measures for accessibility and organization, but not for visibility in some cases. This indicates that even experts have difficulty understanding spatial aspects of an architecture design from 2D blueprints alone.
In vivo use of hyperspectral imaging to develop a noncontact endoscopic diagnosis support system for malignant colorectal tumors

Abstract. The early detection and diagnosis of malignant colorectal tumors enables the initiation of early-stage therapy and can significantly increase the survival rate and post-treatment quality of life among cancer patients. Hyperspectral imaging (HSI) is recognized as a powerful tool for noninvasive cancer detection. In the gastrointestinal field, most of the studies on HSI have involved ex vivo biopsies or resected tissues. In the present study, we aimed to assess the difference in the in vivo spectral reflectance of malignant colorectal tumors and normal mucosa. A total of 21 colorectal tumors or adenomatous polyps from 12 patients at Shanghai Zhongshan Hospital were examined using a flexible hyperspectral (HS) colonoscopy system that can obtain in vivo HS images of the colorectal mucosa. We determined the optimal wavelengths for differentiating tumors from normal tissue based on these recorded images. The application of the determined wavelengths in spectral imaging in clinical trials indicated that such a clinical support system comprising a flexible HS colonoscopy unit and band selection unit is useful for outlining the tumor region and enhancing the display of the mucosa microvascular pattern in vivo.