Miracle-dz/UniFAR-FTUs huggingface dataset:
Study of a possible role of the monoamine oxidase A (MAOA) gene in paranoid schizophrenia among a Chinese population

Monoamine oxidase A (MAOA) is the enzyme responsible for degradation of several monoamines, such as dopamine and serotonin that are considered as being two of the most important neurotransmitters involved in the pathophysiology of schizophrenia. To study a possible role of the MAOA gene in conferring susceptibility to schizophrenia, the present study genotyped the variable number of tandem repeat (VNTR) polymorphism and 41 SNPs across this gene among 555 unrelated patients with paranoid schizophrenia and 567 unrelated healthy controls. Quantitative real‐time PCR analysis was employed to quantify expression of MAOA mRNA in 73 drug‐free patients. While none of these genotyped DNA markers showed allelic association with paranoid schizophrenia, haplotypic association was found for the VNTR‐rs6323, VNTR‐rs1137070, and VNTR‐rs6323‐rs1137070 haplotypes in female subjects. Nevertheless, no significant change of the expression of MAOA mRNA was detected in either female or male patients with paranoid schizophrenia. Our study suggests that the interaction between genetic variants within the MAOA gene may contribute to an increased risk of paranoid schizophrenia, but the precise mechanism needs further investigation. © 2011 Wiley Periodicals, Inc.
Single-Cell STAT5 Signal Transduction Profiling in Normal and Leukemic Stem and Progenitor Cell Populations Reveals Highly Distinct Cytokine Responses

Background Signal Transducer and Activator of Transcription 5 (STAT5) plays critical roles in normal and leukemic hematopoiesis. However, the manner in which STAT5 responds to early-acting and lineage-restricted cytokines, particularly in leukemic stem/progenitor cells, is largely unknown. Methodology/Principal Findings We optimized a multiparametric flow cytometry protocol to analyze STAT5 phosphorylation upon cytokine stimulation in stem and progenitor cell compartments at a single-cell level. In normal cord blood (CB) cells, STAT5 phosphorylation was efficiently induced by TPO, IL-3 and GM-CSF within CD34+CD38− hematopoietic stem cells (HSCs). EPO- and SCF-induced STAT5 phosphorylation was largely restricted to the megakaryocyte-erythroid progenitor (MEP) compartment, while G-CSF as well IL-3 and GM-CSF were most efficient in inducing STAT5 phosphorylation in the myeloid progenitor compartments. Strikingly, mobilized adult peripheral blood (PB) CD34+ cells responded much less efficiently to cytokine-induced STAT5 activation, with the exception of TPO. In leukemic stem and progenitor cells, highly distinct cytokine responses were observed, differing significantly from their normal counterparts. These responses could not be predicted by the expression level of cytokine receptors. Also, heterogeneity existed in cytokine requirements for long-term expansion of AML CD34+ cells on stroma. Conclusions/Significance In conclusion, our optimized multiparametric flow cytometry protocols allow the analysis of signal transduction at the single cell level in normal and leukemic stem and progenitor cells. Our study demonstrates highly distinctive cytokine responses in STAT5 phosphorylation in both normal and leukemic stem/progenitor cells.
Computer-Assisted Interpretation of Planar Whole-Body Bone Scans

The purpose of this study was to develop a computer-assisted diagnosis (CAD) system based on image-processing techniques and artificial neural networks for the interpretation of bone scans performed to determine the presence or absence of metastases. Methods: A training group of 810 consecutive patients who had undergone bone scintigraphy due to suspected metastatic disease were included in the study. Whole-body images, anterior and posterior views, were obtained after an injection of 99mTc-methylene diphosphonate. The image-processing techniques included algorithms for automatic segmentation of the skeleton and automatic detection and feature extraction of hot spots. Two sets of artificial neural networks were used to classify the images, 1 classifying each hot spot separately and the other classifying the whole bone scan. A test group of 59 patients with breast or prostate cancer was used to evaluate the CAD system. The patients in the test group were selected to reflect the spectrum of pathology found in everyday clinical work. As the gold standard for the test group, we used the final clinical assessment of each case. This assessment was based on follow-up scans and other clinical data, including the results of laboratory tests, and available diagnostic images, such as from MRI, CT, and radiography, from a mean follow-up period of 4.8 y. Results: The CAD system correctly identified 19 of the 21 patients with metastases in the test group, showing a sensitivity of 90%. False-positive classification of metastases was made in 4 of the 38 patients not classified as having metastases by the gold standard, resulting in a specificity of 89%. Conclusion: A completely automated CAD system can be used to detect metastases in bone scans. Application of the method as a clinical decision support tool appears to have significant potential.
The effect of biologic therapy different from infliximab or adalimumab in patients with refractory uveitis due to Behçet's disease: results of a multicentre open-label study.

OBJECTIVES To assess the efficacy of other biologic therapies, different from infliximab (IFX) and adalimumab (ADA), in patients with Behçet's disease uveitis (BU). METHODS Multicenter study of 124 patients with BU refractory to at least one standard immunosuppressive agent that required IFX or ADA therapy. Patients who had to be switched to another biologic agent due to inefficacy or intolerance to IFX or ADA or patient's decision were assessed. The main outcome measures were the degree of anterior and posterior chamber inflammation and macular thickness. RESULTS Seven (5.6%) of 124 cases (4 women/3 men; mean age, 43 (range 28- 67) years; 12 affected eyes) were studied. Five of them had been initially treated with ADA and 2 with IFX. The other biologic agents used were golimumab (n=4), tocilizumab (n=2) and rituximab (n=1). The ocular pattern was panuveitis (n=4) or posterior uveitis (n=3). Uveitis was bilateral in 5 patients (71.4%). At baseline, anterior chamber and vitreous inflammation were present in 6 (50%) and 7 (58.3%) of the eyes. All the patients (12 eyes) had macular thickening (OCT>250μm) and 4 of them (7 eyes), cystoid macular edema (OCT>300 μm). Besides reduction anterior chamber and vitreous inflammation, we observed a reduction of OCT values, from 330.4±58.5 μm at the onset of the biological agent to 273±50 μm at month 12 (p=0.06). Six patients achieved a complete remission of uveitis. CONCLUSIONS The vast majority of patients with BU refractory to standard immunosuppressive drugs are successfully controlled with ADA and/or IFX. Other biologic agents appear to be also useful.
Dark Matter Halos: Velocity Anisotropy-Density Slope Relation

Dark matter (DM) halos formed in CDM cosmologies seem to be characterized by a power-law phase-space density profile. The density of the DM halos is often fitted by the NFW profile but a better fit is provided by the Sersic fitting formula. These relations are empirically derived from cosmological simulations of structure formation but have not yet been explained on a first-principle basis. Here we solve the Jeans equation under the assumption of a spherical DM halo in dynamical equilibrium, that obeys a power-law phase-space density and either the NFW-like or the Sersic density profile. We then calculate the velocity anisotropy, β (r) , analytically. Our main result is that for the NFW-like profile the β − γ relation is not a linear one (where γ is the logarithmic derivative of the density ρ [ r] ). The shape of β (r) depends mostly on the ratio of the gravitational to kinetic energy within the NFW scale radius Rs. For the Sersic profile a linear β − γ relation is recovered, and in particular for the Sersic index of n ≈ 6.0 case the linear fit of Hansen & Moore is reproduced. Our main result is that the phase-space density power law, the Sersic density form, and the linear β − γ dependence constitute a consistent set of relations which obey the spherical Jeans equation and as such provide the framework for the dynamical modeling of DM halos.
International Journal of Behavioral Nutrition and Physical Activity Effect of Point-of-purchase Calorie Labeling on Restaurant and Cafeteria Food Choices: a Review of the Literature

Background: Eating away from home has increased in prevalence among US adults and now comprises about 50% of food expenditures. Calorie labeling on chain restaurant menus is one specific policy that has been proposed to help consumers make better food choices at restaurants. The present review evaluates the available empirical literature on the effects of calorie information on food choices in restaurant and cafeteria settings.
A competing risk approach to the analysis of trials of alternative intra‐uterine devices (IUDs) for fertility regulation

The methods of survival analysis have had a profound influence on the way that studies concerned with the safety and efficacy of intra‐uterine devices (IUDs) for fertility control have been designed, conducted, analysed and reported. For example, the Kaplan–Meier technique has been used to summarize the results of controlled clinical trials of alternative devices and the logrank test used to make any comparisons. A particular feature of IUD studies is the large number of possible causes of failure (reasons for discontinuation of the device). These lead to considerations of competing risks, where in this framework, the discontinuations are considered as competing causes of contraceptive failure. In this context, we compare the net and crude probability estimates of discontinuation rates using continuous time and argue for the routine use of the latter. We use data from a randomized multi‐centre trialon the long‐term safety and efficacy of two IUDs, TCu220C and TCu380A, for illustration. Copyright © 2001 John Wiley & Sons, Ltd.
Multisensory Congruency as a Mechanism for Attentional Control over Perceptual Selection

The neural mechanisms underlying attentional selection of competing neural signals for awareness remains an unresolved issue. We studied attentional selection, using perceptually ambiguous stimuli in a novel multisensory paradigm that combined competing auditory and competing visual stimuli. We demonstrate that the ability to select, and attentively hold, one of the competing alternatives in either sensory modality is greatly enhanced when there is a matching cross-modal stimulus. Intriguingly, this multimodal enhancement of attentional selection seems to require a conscious act of attention, as passively experiencing the multisensory stimuli did not enhance control over the stimulus. We also demonstrate that congruent auditory or tactile information, and combined auditory–tactile information, aids attentional control over competing visual stimuli and visa versa. Our data suggest a functional role for recently found neurons that combine voluntarily initiated attentional functions across sensory modalities. We argue that these units provide a mechanism for structuring multisensory inputs that are then used to selectively modulate early (unimodal) cortical processing, boosting the gain of task-relevant features for willful control over perceptual awareness.
Epirubicin Followed by Cyclophosphamide, Methotrexate and 5-Fluorouracil versus Paclitaxel Followed by Epirubicin and Vinorelbine in Patients with High-Risk Operable Breast Cancer

Objective: Breast cancer patients with >3 involved nodes (N+) have a poor outcome. Chemotherapy (CT), alone or combined with endocrine therapy (ET) in hormone receptor (HOR)-positive patients, is the standard for these women. However, there are still questions surrounding the optimal adjuvant CT regimen. Methods: 244 patients with >3 N+ were randomized to receive either four 3-weekly courses of epirubicin (E: 100 mg/m2, day 1) followed by four 4-weekly cycles of cyclophosphamide, methotrexate and 5-fluorouracil (CMF: 600, 40, 600 mg/m2, days 1, 8: n = 122) or four 3-weekly courses of paclitaxel (T: 175 mg/m2, day 1) followed by four 3-weekly cycles of epirubicin and vinorelbine (E: 75 mg/m2, day 1; V: 25 mg/m2, days 1, 8: n = 122). After CT, tamoxifen (plus an LH-RH analog in menstruating women) was given to all HOR-positive patients over a period of 5 years. Overall survival (OS) was the primary end point. Relapse-free survival (RFS) and toxicity were secondary end points. Results: At a median follow-up time of 102 months (range 3–146), OS and RFS did not differ significantly between groups (E-CMF vs. T-EV: OS, HR 0.94, 95% CI 0.59–1.48, p = 0.8; RFS, HR 0.86, 95% CI 0.57–1.29, p = 0.45). The lack of any difference between assigned treatments was confirmed by multivariate analysis (E-CMF vs. T-EV: RFS, HR 0.98, 95% CI 0.64–1.48, p = 0.9). The 2 regimens showed different toxicity profiles. In fact, significantly more women assigned to E-CMF were affected by stomatitis (p = 0.001) while significantly more women in the T-EV group developed peripheral neuropathy (p < 0.0001) and musculoskeletal disorders (p < 0.0001). However, side effects were moderate and manageable and no toxic death occurred in either arm of the study. Conclusions: T-EV was safe and moderately toxic but was not superior to E-CMF.
Expectations from structural genomics

Structural genomics projects aim to provide an experimental structure or a good model for every protein in all completed genomes. Most of the experimental work for these projects will be directed toward proteins whose fold cannot be readily recognized by simple sequence comparison with proteins of known structure. Based on the history of proteins classified in the SCOP structure database, we expect that only about a quarter of the early structural genomics targets will have a new fold. Among the remaining ones, about half are likely to be evolutionarily related to proteins of known structure, even though the homology could not be readily detected by sequence analysis.
Vestibulocollic reflexes evoked by short‐duration galvanic stimulation in man

1 Vestibular‐dependent responses in leg muscles following transmastoid galvanic stimulation have been well characterized. Here we describe the properties of vestibulocollic responses evoked by transmastoid galvanic stimulation. 2 In twelve healthy human subjects we examined the averaged responses in unrectified sternocleidomastoid (SCM) EMG evoked by transmastoid stimulation using current pulses of 4 mA intensity and 2 ms duration. In ten subjects we also examined the effects of unilateral vestibular stimulation with the indifferent electrode at the vertex. In further experiments we studied the effects of different levels of background muscle activation, head position, current intensity and current duration. We compared these responses with click‐evoked vestibulocollic responses in SCM. 3 A clearly defined biphasic response, beginning with a surface positivity, was recorded in the SCM ipsilateral to the side of cathode placement in all subjects. We refer to this as the p13/n23 [g] (galvanic) response, given the close similarity, in terms of waveform and latencies, to the previously described click‐evoked p13/n23 vestibulocollic response. The amplitude of this response was linearly related to background muscle activation, current intensity and current duration, but independent of head position. Unilateral galvanic stimulation revealed the p13/n23 [g] response to be solely generated by the cathode. 4 A biphasic response beginning with a surface negativity (n12/p20 [g]) contralateral to the cathode was seen in all subjects and was generated by both the cathode contralaterally and the anode ipsilaterally. 5 Both the p13/n23 [g] and n12/p20 [g] potentials were abolished by selective vestibular nerve section and unaffected by severe sensorineural deafness. 6 We conclude that galvanic stimulation evokes short‐latency vestibulocollic reflexes. These vestibulocollic reflexes have properties that are distinct from those described for galvanic‐evoked vestibular reflexes in leg muscles, and which may be related to their differing physiological roles.
The consequences of diversity in multidisciplinary work teams

A heart rate and body temperature measurement system where the temperature is measured by a thermistor placed in intimate contact with the patient which varies in resistance value, the variation being converted to a voltage which is applied to a voltage controlled oscillator forming a pulse train input to a storage register. The register is gated in timed intervals into a digital display utilizing a mode switch and reset switch. The mode switch selects temperature or pulse rate. The pulse rate is determined by transluminating a finger of the patient with a light source such as a bulb and observing coloration changes with a light sensitive device, the signal being applied to an amplifier circuit driving a schmitt trigger, the output being stretched by a monostable multivibrator and the pulses being stored in an integrating amplifier which provides a DC level. The DC level is also applied to the mode switch which is input to the same VCO. The mode switch selects pre-set inputs for the register which again is gated by a timed signal into a digital display. The apparatus described is a portable indicating device.
Studies on fertility of DDK mice: reciprocal crosses between DDK and C57BL/6J strains and experimental transplantation of the ovary.

The fertility of DDK mice was investigated in reciprocal crosses between DDK and C57BL/6J strains of mice. When mated to C57BL males, DDK females were semi-sterile. By contrast, when C57BL females were mated to DDK males, they showed better fertility than in the intra-strain mating. Embryological study has revealed that the low fertility of DDK females is caused by embryonic death at the morulablastocyst or pre-egg cylinder stage, 3 to 5 days after copulation. A defect in trophoblast formation was a common feature of the dead embryos. Experiments involving ovary transplantation have shown that the
Engineering of chromosomal wax ester synthase integrated Saccharomyces cerevisiae mutants for improved biosynthesis of fatty acid ethyl esters

In recent years, significant advances have been made to engineer robust microbes for overproducing biochemical products from renewable resources. These accomplishments have to a large extend been based on plasmid based methods. However, plasmid maintenance may cause a metabolic burden on the host cell and plasmid‐based overexpression of genes can result in genetically unstable strains, which contributes to loss in productivity. Here, a chromosome engineering method based on delta integration was applied in Saccharomyces cerevisiae for the production of fatty acid ethyl esters (FAEEs), which can be directly used as biodiesel and would be a possible substitute for conventional petroleum‐based diesel. An integration construct was designed and integrated into chromosomal delta sequences by repetitive transformation, which resulted in 1–6 copies of the integration construct per genome. The corresponding FAEE production increased up to 34 mg/L, which is an about sixfold increase compared to the equivalent plasmid‐based producer. The integrated cassette in the yeast genome was stably maintained in nonselective medium after deletion of RAD52 which is essential for efficient homologous recombination. To obtain a further increase of FAEE production, genes encoding endogenous acyl‐CoA binding protein (ACB1) and a bacterial NADP+‐dependent glyceraldehyde‐3‐phosphate dehydrogenase (gapN) were overexpressed in the final integration strain, which resulted in another 40% percent increase in FAEE production. Our integration strategy enables easy engineering of strains with adjustable gene copy numbers integrated into the genome and this allows for an easy evaluation of the effect of the gene copy number on pathway flux. It therefore represents a valuable tool for introducing and expressing a heterologous pathway in yeast. Biotechnol. Bioeng. 2014;111: 1740–1747. © 2014 Wiley Periodicals, Inc.
Adequacy of laser diffraction for soil particle size analysis

Sedimentation has been a standard methodology for particle size analysis since the early 1900s. In recent years laser diffraction is beginning to replace sedimentation as the prefered technique in some industries, such as marine sediment analysis. However, for the particle size analysis of soils, which have a diverse range of both particle size and shape, laser diffraction still requires evaluation of its reliability. In this study, the sedimentation based sieve plummet balance method and the laser diffraction method were used to measure the particle size distribution of 22 soil samples representing four contrasting Australian Soil Orders. Initially, a precise wet riffling methodology was developed capable of obtaining representative samples within the recommended obscuration range for laser diffraction. It was found that repeatable results were obtained even if measurements were made at the extreme ends of the manufacturer’s recommended obscuration range. Results from statistical analysis suggested that the use of sample pretreatment to remove soil organic carbon (and possible traces of calcium-carbonate content) made minor differences to the laser diffraction particle size distributions compared to no pretreatment. These differences were found to be marginally statistically significant in the Podosol topsoil and Vertosol subsoil. There are well known reasons why sedimentation methods may be considered to ‘overestimate’ plate-like clay particles, while laser diffraction will ‘underestimate’ the proportion of clay particles. In this study we used Lin’s concordance correlation coefficient to determine the equivalence of laser diffraction and sieve plummet balance results. The results suggested that the laser diffraction equivalent thresholds corresponding to the sieve plummet balance cumulative particle sizes of < 2 μm, < 20 μm, and < 200 μm, were < 9 μm, < 26 μm, < 275 μm respectively. The many advantages of laser diffraction for soil particle size analysis, and the empirical results of this study, suggest that deployment of laser diffraction as a standard test procedure can provide reliable results, provided consistent sample preparation is used.
Enzyme immunoassay using a novel recombinant polypeptide to detect human immunodeficiency virus env antibody

A unique antigen, CBre3, has been synthesized from a genetically engineered clone to detect human immunodeficiency virus (HIV) env antibodies with high sensitivity and specificity. The antigen contains sequences derived from both envelope proteins of HIV, i.e., gp120 and gp41, and was purified free of Escherichia coli proteins detectable by Coomassie stain or immunoblotting with E. coli antiserum. The purified recombinant polypeptides were used as antigen in an enzyme immunoassay (EIA) to screen serum samples from healthy and HIV-infected individuals. The same samples were also tested by radioimmunoprecipitation (RIP) for gp120 and gp160 HIV antibodies. All samples containing gp120 and gp160 antibodies by RIP had CBre3 EIA values greater than 0.35 (n, 122; range, 0.37 to 2.1+; median, 1.65). All RIP HIV antibody-negative samples had CBre3 EIA values less than 0.25 (n, 140; mean, 0.052; standard deviation, 0.045; range, 0.00 to 0.22). The endpoint titer of a standard positive control serum was 1:10,000 by RIP and by CBre3 EIA. The assay was 100% accurate in three proficiency panels. It easily detected six samples from individuals whose infections were confirmed by culture; these samples were reactive only with p24 by Western blot. The samples also were positive for gp120 and gp160 antibodies by RIP. These data suggest that the CBre3 EIA can detect env antibodies as sensitively and specifically as RIP and with more sensitivity than Western blot.