Miracle-dz/UniFAR-FTUs huggingface dataset:
Striatal Molecular Signature of Subchronic Subthalamic Nucleus High Frequency Stimulation in Parkinsonian Rat

This study addresses the molecular mechanisms underlying the action of subthalamic nucleus high frequency stimulation (STN-HFS) in the treatment of Parkinson's disease and its interaction with levodopa (L-DOPA), focusing on the striatum. Striatal gene expression profile was assessed in rats with nigral dopamine neuron lesion, either treated or not, using agilent microarrays and qPCR verification. The treatments consisted in anti-akinetic STN-HFS (5 days), chronic L-DOPA treatment inducing dyskinesia (LIDs) or the combination of the two treatments that exacerbated LIDs. STN-HFS modulated 71 striatal genes. The main biological processes associated with the differentially expressed gene products include regulation of growth, of apoptosis and of synaptic transmission, and extracellular region is a major cellular component implicated. In particular, several of these genes have been shown to support survival or differentiation of striatal or of dopaminergic neurons. These results indicate that STN HFS may induce widespread anatomo-functional rearrangements in the striatum and create a molecular environment favorable for neuroprotection and neuroplasticity. STN-HFS and L-DOPA treatment share very few common gene regulation features indicating that the molecular substrates underlying their striatal action are mostly different; among the common effects is the down-regulation of Adrb1, which encodes the adrenergic beta-1- receptor, supporting a major role of this receptor in Parkinson's disease. In addition to genes already reported to be associated with LIDs (preprodynorphin, thyrotropin-releasing hormone, metabotropic glutamate receptor 4, cannabinoid receptor 1), the comparison between DOPA and DOPA/HFS identifies immunity-related genes as potential players in L-DOPA side effects.
Identifying Cheating on Unproctored Internet Tests: The Z-Test and the Likelihood Ratio Test

Unproctored Internet testing (UIT) is becoming more popular in employment settings due to its cost effectiveness and efficiency. However, one of the major concerns with UIT is the possibility of cheating behaviors: a more capable conspirator can sit beside the real applicant and answer test items, or the applicant may use unauthorized materials. The present study examined the effectiveness of using a proctored verification test following the UIT to identify cheating in UIT, where 2 test statistics, a Z-test and a likelihood ratio (LR) test, compare the consistency of test performance across the testing conditions. A simulation study was conducted to test the effectiveness of the two test statistics for a computerized adaptive test format. Results indicate that both test statistics have high power to detect dishonest job applicants at low Type I error rates. Compared with the LR test, the Z-test was more efficient and effective and is therefore recommended for practical applications. The theoretical and practical implications are discussed.
A retrospective cohort study of the utility of the modified early warning score for interfacility transfer of patients with traumatic injury

Objective The modified early warning score (MEWS) is a ‘track and trigger’ score using routine physiological vital signs. The objective is to determine if the pretransfer MEWS can be used for predicting outcomes in trauma patients requiring interfacility transfer to higher levels of care. Design, setting and participants Retrospective study of consecutively transferred trauma patients into a level-II trauma centre from 2013 to 2014. Interventions None. Outcome measures Mortality, intensive care unit (ICU) admission, operative procedure, MEWS deterioration in-transit, air transport interfacility, secondary overtriage (low injury severity score (ISS) <10, LOS<1 day, discharged home) and severe injury (ISS ≥16). The association between the pretransfer MEWS and outcomes were analysed with Cochran-Armitage trend tests, receiver operator characteristic (ROC) curves and univariate logistic regression. Results There were 587 transferred patients; outcomes were reported in 339 patients with complete data on all five vital signs used to calculate the MEWS. The MEWS ranged from 0 to 9 (median of 1). There was a significant linear relationship between MEWS and study outcomes, especially mortality, ICU admission, air medical transport and severe injury (p<0.001 for all). A threshold score ≥4 was identified by ROC analysis; 11.2% of patients had MEWS ≥4. Outcomes were significantly worse in patients with MEWS ≥4 versus <4: mortality (26.2% vs 3.0%, OR=11.59, p<0.001); ICU admission (73.7% vs 47.2%, OR=3.14, p=0.003); air transfer (42.1% vs 15.6%, OR=3.93, p<0.001) and severe injury (59.5% vs 27.2%, OR=3.9, p<0.001). The MEWS was not associated with surgery, in-transit MEWS deterioration or secondary overtriage. Conclusion Pretransfer MEWS ≥4 may be used by the receiving facility for predicting injury severity, mortality, air transport and ICU resource use. In the interfacility transport setting, the MEWS may be useful for identifying patients with less obvious need for transfer or requiring more expeditious transfer.
Aging, Emotion, and Evolution

Abstract: Ample empirical evidence shows that basic cognitive processes integral to learning and memory suffer with age. Explanations for age‐related loss typically cite the absence of evolutionary selection pressures during the postreproductive years, which consequently failed to optimize functioning during old age. In this paper, we suggest that evolutionary pressures did operate at older ages and that an evolutionary account is entirely consistent with the pattern of findings currently available in the psychological literature on aging. Cognitive loss is limited primarily to new learning, yet integrated world knowledge increases with age. In addition, socioemotional regulation improves with age, which is associated with increased investment in emotionally meaningful others (most notably kin). In this chapter, we argue that this profile of late‐life characteristics contributes to the reproductive success of kin. We consider how the uniquely human ability to monitor place in the life cycle and the consequent motivational shifts that occur when boundaries in time are perceived contribute to the adaptive value of long life. Finally, we suggest that joint consideration of evolutionary theory and life‐span psychology can lead to fruitful advances in the understanding of human aging.
Fast Solving Maximum Weight Clique Problem in Massive Graphs

This paper explores techniques for fast solving the maximum weight clique problem (MWCP) in very large scale real-world graphs. Because of the size of such graphs and the intractability of MWCP, previously developed algorithms may not be applicable. Although recent heuristic algorithms make progress in solving MWCP in massive graphs, they still need considerable time to get a good solution. In this work, we propose a new method for MWCP which interleaves between clique construction and graph reduction. We also propose three novel ideas to make it efficient, and develop an algorithm called FastWClq. Experiments on massive graphs from various applications show that, Fast-WClq finds better solutions than state of the art algorithms while the run time is much less. Further, FastWClq proves the optimal solution for about half of the graphs in an averaged time less than one second.
Connecting strongly correlated superfluids by a quantum point contact

Simulating electronic transport with atoms Two superconductors connected by a bridge made out of nonsuperconducting material form a so-called Josephson junction (see the Perspective by Belzig). Valtolina et al. replaced the superconductors with two reservoirs of a superfluid Fermi gas and connected them by a weak link to allow atoms to move from one side to the other. Then they made one reservoir more populated than the other and studied the ensuing dynamics as a function of interaction strength between the atoms. In a related experiment, Husmann et al. kept the interaction strength at its maximum, but varied the temperature and the properties of the link. As temperature increased, the superfluid disappeared and thermal transport took over. Science, this issue p. 1498, p. 1505; see also p. 1470 Two reservoirs of strongly interacting fermionic 6Li atoms are used to simulate a mesoscopic electronic device. [Also see Perspective by Belzig] Point contacts provide simple connections between macroscopic particle reservoirs. In electric circuits, strong links between metals, semiconductors, or superconductors have applications for fundamental condensed-matter physics as well as quantum information processing. However, for complex, strongly correlated materials, links have been largely restricted to weak tunnel junctions. We studied resonantly interacting Fermi gases connected by a tunable, ballistic quantum point contact, finding a nonlinear current-bias relation. At low temperature, our observations agree quantitatively with a theoretical model in which the current originates from multiple Andreev reflections. In a wide contact geometry, the competition between superfluidity and thermally activated transport leads to a conductance minimum. Our system offers a controllable platform for the study of mesoscopic devices based on strongly interacting matter.
Consistently Low Prevalence of Syphilis among Female Sex Workers in Jinan, China: Findings from Two Consecutive Respondent Driven Sampling Surveys

Background Routine surveillance using convenient sampling found low prevalence of HIV and syphilis among female sex workers in China. Two consecutive surveys using respondent driven sampling were conducted in 2008 and 2009 to examine the prevalence of HIV and syphilis among female sex workers in Jinan, China. Methods A face-to-face interview was conducted to collect demographic, behavioral and service utilization information using a structured questionnaire. Blood samples were drawn for serological tests of HIV-1 antibody and syphilis antibody. Respondent Driven Sampling Analysis Tool was used to generate population level estimates. Results In 2008 and in 2009, 363 and 432 subjects were recruited and surveyed respectively. Prevalence of syphilis was 2.8% in 2008 and 2.2% in 2009, while no HIV case was found in both years. Results are comparable to those from routine sentinel surveillance system in the city. Only 60.8% subjects in 2008 and 48.3% in 2009 reported a consistent condom use with clients during the past month. Over 50% subjects had not been covered by any HIV-related services in the past year, with only 15.6% subjects in 2008 and 13.1% in 2009 ever tested for HIV. Conclusions Despite the low prevalence of syphilis and HIV, risk behaviors are common. Targeted interventions to promote the safe sex and utilization of existing intervention services are still needed to keep the epidemic from growing.
Profile of the level of physical activity in the daily lives of patients with COPD in Brazil.

OBJECTIVE To evaluate characteristics of physical activities in daily life in COPD patients in Brazil, correlating those characteristics with physiological variables. METHODS Physical activities in daily life were evaluated in 40 COPD patients (18 males; 66 + or - 8 years of age; FEV(1) = 46 + or - 16 % of predicted; body mass index = 27 + or - 6 kg/m(2)) and 30 healthy age- and gender-matched subjects, using a multiaxial accelerometer-based sensor for 12 h/day on two consecutive days. We also assessed maximal and functional exercise capacity, using the incremental exercise test and the six-minute walk test (6MWT), respectively; MIP and MEP; peripheral muscle force, using the one-repetition maximum test and the handgrip test; quality of life, using the Saint George's Respiratory Questionnaire (SGRQ); functional status, using the London Chest Activity of Daily Living questionnaire; and dyspnea sensation, using the Medical Research Council (MRC) scale. RESULTS Mean walking time/day was shorter for COPD patients than for the controls (55 + or - 33 vs. 80 + or - 28 min/day; p = 0.001), as movement intensity was lower (1.9 + or - 0.4 vs. 2.3 + or - 0.6 m/s(2); p = 0.004). The COPD patients also tended to spend more time seated (294 + or - 114 vs. 246 + or - 122 min/day, p = 0.08). Walking time/day correlated with the 6MWT (r = 0.42; p = 0.007) and maximal workload (r = 0.41; p = 0.009), as well as with age, MRC scale score and SGRQ activity domain score (-0.31 < or = r < or = -0.43; p < or = 0.05 for all). CONCLUSIONS This sample of Brazilian patients with COPD, although more active than those evaluated in studies conducted in Europe, were less active than were the controls. Walking time/day correlated only moderately with maximal and functional exercise capacity.
The pragmatics of humor support

Abstract Many researchers claim joking and laughter to be an adjacency pair. There are, however, a range of strategies used for supporting humor in conversation, of which laughter is just one. This paper uses natural conversational data to illustrate a variety of humor support strategies. Common support strategies include contributing more humor, playing along with the gag, using echo or overlap, offering sympathy and contradicting self-deprecating humor. There are four implicatures associated with full support of humor: recognition of a humorous frame, understanding the humor, appreciating the humor, and agreeing with any message associated with it. Recognition, understanding and appreciation are in an entailment relationship, and this relationship can be exploited to display recognition and understanding while denying appreciation. The implicature of agreement is particularly salient when teasing or self-deprecating humor is being supported.
Association of Smoking Status With Quality of Life in a Cross-sectional Population-Based Sample of Iranian Adults

This study aimed to examine the association between smoking status and quality of life (QOL) in a population-based sample of Iranian adults. The study used data from the Isfahan Healthy Heart Program (IHHP). A total of 5830 participants were included. They were categorized as current, former, or never smokers. QOL was evaluated using the World Health Organization Quality of Life Questionnaire (WHOQOL-BREF), Persian version. Data entry was performed using SPSS (SPSS Inc, Chicago, IL) software version 13.0. QOL was associated with marital status (P = .014), education (P = .001), occupation (P = .007), and income (P < .000) among current smokers. After adjusting for sociodemographic characteristics, smoking decreased QOL among current (odds ratio [OR] = 0.98; 95% confidence interval [CI] = 0.97-0.99) and past (OR = 0.97; 95%CI = 0.95-0.98) smokers relative to never smokers. QOL is lower among Iranian current and past smokers, and the relation is independent of underlying sociodemographic factors.
Loss of LAP2α Delays Satellite Cell Differentiation and Affects Postnatal Fiber‐Type Determination

Lamina‐associated polypeptide 2α (LAP2α) is a nucleoplasmic protein implicated in cell cycle regulation through its interaction with A‐type lamins and the retinoblastoma protein. Mutations in lamin A/C and LAP2α cause late onset striated muscle diseases, but the molecular mechanisms are poorly understood. To study the role of LAP2α in skeletal muscle function and postnatal tissue homeostasis, we generated complete and muscle‐specific LAP2α knockout mice. Whereas overall muscle morphology, function, and regeneration were not detectably affected, the myofiber‐associated muscle stem cell pool was increased in complete LAP2α knockout animals. At molecular level, the absence of LAP2α preserved the stem cell‐like phenotype of Lap2α−/− primary myoblasts and delayed their in vitro differentiation. In addition, loss of LAP2α shifted the myofiber‐type ratios of adult slow muscles toward fast fiber types. Conditional Cre‐mediated late muscle‐specific ablation of LAP2α affected early stages of in vitro myoblast differentiation, and also fiber‐type determination, but did not change myofiber‐associated stem cell numbers in vivo. Our data demonstrate multiple and distinct functions of LAP2α in muscle stem cell maintenance, early phases of myogenic differentiation, and muscle remodeling. STEM CELLS 2010;28:480–488
The Clostridium difficile Protease Cwp84 Modulates both Biofilm Formation and Cell-Surface Properties

Clostridium difficile is responsible for 15-20% of antibiotic-associated diarrheas, and nearly all cases of pseudomembranous colitis. Among the cell wall proteins involved in the colonization process, Cwp84 is a protease that cleaves the S-layer protein SlpA into two subunits. A cwp84 mutant was previously shown to be affected for in vitro growth but not in its virulence in a hamster model. In this study, the cwp84 mutant elaborated biofilms with increased biomass compared with the parental strain, allowing the mutant to grow more robustly in the biofilm state. Proteomic analyses of the 630Δerm bacteria growing within the biofilm revealed the distribution of abundant proteins either in cell surface, matrix or supernatant fractions. Of note, the toxin TcdA was found in the biofilm matrix. Although the overall proteome differences between the cwp84 mutant and the parental strains were modest, there was still a significant impact on bacterial surface properties such as altered hydrophobicity. In vitro and in vivo competition assays revealed that the mutant was significantly impaired for growth only in the planktonic state, but not in biofilms or in vivo. Taken together, our results suggest that the phenotypes in the cwp84 mutant come from either the accumulation of uncleaved SlpA, or the ability of Cwp84 to cleave as yet undetermined proteins.
Ion Channel Stochasticity May Be Critical in Determining the Reliability and Precision of Spike Timing

The firing reliability and precision of an isopotential membrane patch consisting of a realistically large number of ion channels is investigated using a stochastic Hodgkin-Huxley (HH) model. In sharp contrast to the deterministic HH model, the biophysically inspired stochastic model reproduces qualitatively the different reliability and precision characteristics of spike firing in response to DC and fluctuating current input in neocortical neurons, as reported by Mainen & Sejnowski (1995). For DC inputs, spike timing is highly unreliable; the reliability and precision are significantly increased for fluctuating current input. This behavior is critically determined by the relatively small number of excitable channels that are opened near threshold for spike firing rather than by the total number of channels that exist in the membrane patch. Channel fluctuations, together with the inherent bistability in the HH equations, give rise to three additional experimentally observed phenomena: subthreshold oscillations in the membrane voltage for DC input, spontaneous spikes for subthreshold inputs, and missing spikes for suprathreshold inputs. We suggest that the noise inherent in the operation of ion channels enables neurons to act as smart encoders. Slowly varying, uncorrelated inputs are coded with low reliability and accuracy and, hence, the information about such inputs is encoded almost exclusively by the spike rate. On the other hand, correlated presynaptic activity produces sharp fluctuations in the input to the postsynaptic cell, which are then encoded with high reliability and accuracy. In this case, information about the input exists in the exact timing of the spikes. We conclude that channel stochasticity should be considered in realistic models of neurons.
Recon: Verifying file system consistency at runtime

File system bugs that corrupt metadata on disk are insidious. Existing reliability methods, such as checksums, redundancy, or transactional updates, merely ensure that the corruption is reliably preserved. Typical workarounds, based on using backups or repairing the file system, are painfully slow. Worse, the recovery may result in further corruption. We present Recon, a system that protects file system metadata from buggy file system operations. Our approach leverages file systems that provide crash consistency using transactional updates. We define declarative statements called consistency invariants for a file system. These invariants must be satisfied by each transaction being committed to disk to preserve file system integrity. Recon checks these invariants at commit, thereby minimizing the damage caused by buggy file systems. The major challenges to this approach are specifying invariants and interpreting file system behavior correctly without relying on the file system code. Recon provides a framework for file-system specific metadata interpretation and invariant checking. We show the feasibility of interpreting metadata and writing consistency invariants for the Linux ext3 file system using this framework. Recon can detect random as well as targeted file-system corruption at runtime as effectively as the offline e2fsck file-system checker, with low overhead.
Added Value Measures in Education Show Genetic as Well as Environmental Influence

Does achievement independent of ability or previous attainment provide a purer measure of the added value of school? In a study of 4000 pairs of 12-year-old twins in the UK, we measured achievement with year-long teacher assessments as well as tests. Raw achievement shows moderate heritability (about 50%) and modest shared environmental influences (25%). Unexpectedly, we show that for indices of the added value of school, genetic influences remain moderate (around 50%), and the shared (school) environment is less important (about 12%). The pervasiveness of genetic influence in how and how much children learn is compatible with an active view of learning in which children create their own educational experiences in part on the basis of their genetic propensities.