Miracle-dz/UniFAR-FTUs huggingface dataset:
Photoinactivation of Ammonia Oxidation in Nitrosomonas

Photoinactivation of ammonia oxidation in cells of Nitrosomonas was shown to follow first-order kinetics with a rate constant proportional to incident light intensity. The action spectrum for photoinactivation consisted of a broad peak in the ultraviolet range, where both hydroxylamine and ammonia oxidation were affected, and a shoulder at approximately 410 nm where only ammonia oxidation was affected. In photoinactivated cells, hydroxylamine but not ammonia was oxidized to nitrite and hydroxylamine but not ammonia caused reduction of cytochromes in vivo. The amount per cell of the following constituents was not measurably altered by photoinactivation: cytochromes b, c, a, and P460; ubiquinone; phospholipid; free amino acids; hydroxylamine-dependent nitrite synthetase; nitrite reductase; p-phenylenediamine oxidase; and cytochrome c oxidase. Malonaldehyde or lipid peroxides were not detected in photoinactivated cells. Photoinactivation was prevented (i) under anaerobic conditions, (ii) in the presence of methanol, allylthiourea, thiosemicarbazide, hydroxylamine, ethylxanthate, or CO at concentrations wich caused 100% inhibition of ammonia oxidation, and (iii) at concentrations of ammonia or hydroxylamine which gave a rapid rate of nitrite production. Recovery of ammonia oxidation activity in 90% inactivated cells took place in 6 h, required an energy and/or nitrogen source, and was inhibited by 400 μg of chloramphenicol per ml.
Non-EST based prediction of exon skipping and intron retention events using Pfam information

Most of the known alternative splice events have been detected by the comparison of expressed sequence tags (ESTs) and cDNAs. However, not all splice events are represented in EST databases since ESTs have several biases. Therefore, non-EST based approaches are needed to extend our view of a transcriptome. Here, we describe a novel method for the ab initio prediction of alternative splice events that is solely based on the annotation of Pfam domains. Furthermore, we applied this approach in a genome-wide manner to all human RefSeq transcripts and predicted a total of 321 exon skipping and intron retention events. We show that this method is very reliable as 78% (250 of 321) of our predictions are confirmed by ESTs or cDNAs. Subsequent analyses of splice events within Pfam domains revealed a significant preference of alternative exon junctions to be located at the protein surface and to avoid secondary structure elements. Thus, splice events within Pfams are probable to alter the structure and function of a domain which makes them highly interesting for detailed biological investigation. As Pfam domains are annotated in many other species, our strategy to predict exon skipping and intron retention events might be important for species with a lower number of ESTs.
Hla Simulation of Agent-based Bacterial Models

Modelling and simulation form an integral part of a predictive and explanatory approach to computational systems biology. However the development of such complex simulation applications is extremely challenging, typically requiring collabora-tive effort from researchers with different domain knowledge and expertise, often at different locations. We believe these challenges can potentially be met by a combination of two emerging standards and their supporting middleware: the Grid and the High Level Architecture. To evaluate the suitability of the HLA as a standard for systems biology simulations on the Grid, we are developing a prototype HLA-compliant, Grid-based simulator for systems biology which we call BACGRID. In this paper, we focus on the distribution of BACGRID using HLA. We outline the systems biology problem we have adopted as a test case, briefly describe the simulation model and the architecture of the simulator, and present preliminary results of experiments to investigate the scalability of the BACGRID HLA distribution.
Bioefficacy, dissipation kinetics and safety evaluation of selected insecticides in Allium cepa L

This paper reports the bioefficacy of selected insecticides against thrips and their pre-harvest intervals (PHI) in onion pertaining to their recommended application rates and maximum residue limits. Profenophos, methomyl and imidacloprid showed comparatively higher bioefficacy against thrips. GC-MS and LC-MS/MS-based residue analysis methods in onion bulbs and composite matrix of bulbs+leaves were thoroughly validated. The residue data for bulb+leaves was assessed with reference to the EU-MRLs applicable for spring onion. Dimethoate was the most stable chemical with PHI of 52.5 days, followed by monocrotophos (24 days) and carbofuran (20.5 days). The PHIs of profenophos, chlorpyrifos, methomyl and cypermethrin were similar and within the range of 10–13 days. Imidacloprid and λ-cyhalothrin had similar PHI of 4.5 days. Spinosad was the fastest-degrading chemical with PHI of 2 days. The combined bioefficacy and residue dynamics information will support label-claim of these insecticides for the management of thrips in onion, help in scheduling their applications in pest management program as per relative PHIs and minimize the residue accumulations at harvest. The dietary exposure was less than the maximum permissible intake for most of the insecticides on all sampling days except for dimethoate and monocrotophos.
German Validation of the Conners Adult ADHD Rating Scale–Self-Report

Objective: The Conners Adult ADHD Rating Scales (CAARS) assess symptoms specific to adults that are frequently used and have been translated into German. The current study tests the factor structure of the CAARS in a large sample of German adults with ADHD and compares the means of the CAARS subscales with those of healthy German controls. Method: CAARS were completed by 466 participants with ADHD and 851 healthy control participants. Confirmatory factor analysis was used to establish model fit with the American original. Comparisons between participants with ADHD and healthy controls and influences of gender, age, and degree of education were analyzed. Results: Confirmatory factor analysis showed a very good fit with the model for the American original. Differences between ADHD participants and healthy controls on all Conners Adult ADHD Rating Scales–Self-Report (CAARS-S) subscales were substantial and significant. Conclusion: The factor structure of the original American model was successfully replicated in this sample of adult German ADHD participants.
Tooth loss in permanent dentition in a rural population, Ninevah Governorate-Iraq.

This study was designed to investigate the prevalence of tooth loss among Iraqi population living ni rural areas, Nineveh Governate. A random sample of 746 subjects, compromising 280 males and 466 females were chosen from two villages ni Nineveh Governate, their age range 5-70 years and it was divided into 6 age groups with an interval of 10 years. The study shows that the percent of individuals with the tooth loss increase with increasing the age, also their mean of teeth loss increase with the increasing age, also their mean of tooth loss per individuals was increase significantly with the increasing age. The result indicated that the females reported higher percent than the males, also the mean of tooth loss were higher significantly ni females than males, the study revealed that the teeth loss ni the upper jaws slightly higher than the lower jaw for the age group 25 years and over, while for younger age group the opposite si true. The first molars recorded the higher percent of tooth loss among the other teeth. The study indicated that the more than 90% of the total individuals that had loss their teeth need fixed or removable prosthodontics treatment.
[Parietal lobes].

From the combination of knowledge and actions, someone can improve their skill and ability. It will lead them to live and work much better. This is why, the students, workers, or even employers should have reading habit for books. Any book will give certain knowledge to take all benefits. This is what this parietal lobes tells you. It will add more knowledge of you to life and work better. Try it and prove it.
Biomass Burning Markers and Residential Burning in the WINTER Aircraft Campaign

As part of the WINTER (Wintertime Investigation of Transport, Emissions, and Reactivity) campaign, a Particle‐into‐Liquid Sampler with a fraction collector was flown aboard the National Center for Atmospheric Research C‐130 aircraft. Two‐minute integrated liquid samples containing dissolved fine particulate matter (PM1) species were collected and analyzed off‐line for the smoke marker levoglucosan using high‐performance anion‐exchange chromatography‐pulsed amperometric detection to compare levoglucosan with aerosol mass spectrometer (AMS) biomass burning markers and investigate the contribution from residential burning during the study. Levoglucosan was correlated with AMS organic aerosol (R2 = 0.49) and with carbon monoxide (CO; R2 = 0.51) for all flights. Levoglucosan was not correlated with the inorganic smoke marker water‐soluble potassium but was correlated with the AMS markers ∆C2H4O2+ (high resolution, R2 = 0.60) and ∆m/z 60 (unit mass resolution, R2 = 0.61). However, at low levoglucosan, AMS markers deviated potentially due to interferences from other sources or differences with the species captured by the AMS markers. Analysis of levoglucosan changes relative to carbon monoxide as plumes advected from source regions showed no systematic levoglucosan loss for plumes up to 20 hr old. Based on literature residential burning source ratios and measured levoglucosan, contributions of organic carbon (OC) due to residential burning were estimated. The contribution ranged from ~30 to 100% of the OC, with significant variability depending on the source ratio used; however, the results show that biomass burning was a significant PM1 OC source across the entire sampling region. A GEOS‐Chem model simulation predicted significantly less smoke contribution.
Low-molecular weight heparin for deep vein thrombosis prophylaxis in hospitalized medical patients: results from a cost-effectiveness analysis

Multiple options exist for the prevention of deep vein thrombosis (DVT) in medical inpatients. We sought to determine the cost-effectiveness of low-molecular-weight heparin (LMWH) relative to unfractionated heparin (UFH) for DVT prevention in this setting. We conducted a cost-effectiveness analysis from the perspective of a third-party payer employing a decision model and literature-based estimates for inputs. In the base-case analysis, LMWH had little impact on the rate of DVT. Despite higher acquisition costs, however, LMWHs resulted in net savings. Routine use of LMWH saves approximately US$89 per patient. The lower rate of heparin-induced thrombocytopenia (HIT) with LMWH accounted for this differential. Univariate sensitivity analysis revealed the model was moderately sensitive to the odds ratio of HIT with LMWH and the cost of HIT. Multivariate sensitivity analysis confirmed the LMWH approach dominated financially. ‘Worst-case’ scenario modeling, where LMWH actually increased the risk for DVT, had little effect on the rate of HIT, and was substantially more costly than UFH, still demonstrated that LMWHs were economically superior. Monte-Carlo simulation indicated the 95% confidence interval around the estimate for savings with LMWH ranged from US$7 to US$373. We conclude that, despite their higher cost, LMWHs for thromboprophylaxis in medical patients result in savings.
Inhibitory Effects of Norwogonin, Oroxylin A, and Mosloflavone on Enterovirus 71

Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxylin A, and mosloflavone from Scutellaria baicalensis Georgi was evaluated using a cytopathic effect (CPE) reduction method, which demonstrated that all three compounds possessed strong anti-EV71 activity and decreased the formation of visible CPEs. Norwogonin, oroxylin A, and mosloflavone also inhibited virus replication during the initial stage of virus infection, and they inhibited viral VP2 protein expression, thereby inhibiting viral capsid protein synthesis. However, ribavirin has a relatively weaker efficacy compared to the other drugs. Therefore, these findings provide important information that will aid in the utilization of norwogonin, oroxylin A, and mosloflavone for EV71 treatment.
Alteration of the Canine Small-Intestinal Lactic Acid Bacterium Microbiota by Feeding of Potential Probiotics

ABSTRACT Five potentially probiotic canine fecal lactic acid bacterium (LAB) strains, Lactobacillus fermentum LAB8, Lactobacillus salivarius LAB9, Weissella confusa LAB10, Lactobacillus rhamnosus LAB11, and Lactobacillus mucosae LAB12, were fed to five permanently fistulated beagles for 7 days. The survival of the strains and their potential effects on the indigenous intestinal LAB microbiota were monitored for 17 days. Denaturing gradient gel electrophoresis (DGGE) demonstrated that the five fed LAB strains survived in the upper gastrointestinal tract and modified the dominant preexisting indigenous jejunal LAB microbiota of the dogs. When the LAB supplementation was ceased, DGGE analysis of jejunal chyme showed that all the fed LAB strains were undetectable after 7 days. However, the diversity of the intestinal indigenous microbiota of the dogs, as characterized from jejunal chyme plated on Lactobacillus selective medium without acetic acid, was reduced and did not return to the original level during the study period. In all but one dog, an indigenous Lactobacillus acidophilus strain emerged as the dominant LAB strain. In conclusion, strains LAB8 to LAB12 have potential as probiotic strains for dogs as they survive in and dominate the jejunal LAB microbiota during feeding and have the ability to modify the intestinal microbiota.
Dynamic pricing , product innovation , and quality-based cost

Manufacturing and high-tech firms simultaneously conduct dynamic pricing and product innovation policies. We model these policies in an optimal control framework. On the supply-side, the firm conducts the pricing and the product innovation policies. The cost of production is related to product quality. On the demand-side, the consumer has interest in price and quality. We analyse the determinants of dynamic pricing and the effect of quality on price. We show that the dynamic pricing is only linked to the characteristics of the demand. Under a general demand function, price dynamics is ambiguous; price may decrease even if quality and cost increase. Under an additive separable demand function, price dynamics follows quality dynamics; the pricing curve emulates the quality curve. Under a multiplicative separable demand function, price dynamics follows cost dynamics; the pricing curve emulates the cost curve.
Low Diet Quality and Nutritional Knowledge in Women with Endometriosis: A Pilot Study

Endometriosis is a chronic gynecological disease characterized by ectopic endometrial tissue outside the uterus that can cause chronic pain, dysmenorrhea, fatigue, and infertility. The aim of this study was to assess diet quality and nutritional knowledge of women with endometriosis. A questionnaire was used to collect data on lifestyle, dietary habits and nutritional knowledge from 90 women. More than 80% of women with endometriosis (82.2%) confirmed that diet and lifestyle are related to the progression of endometriosis. After being diagnosed with endometriosis, 77.3% of respondents reported a change in their dietary habits and made various changes to their diet by increasing or eliminating the consumption of certain foods. The majority declared reducing pro-inflammatory products, increasing anti-inflammatory foods, including the consumption of vegetables and fruits, avoiding white/or red/or processed meat, and avoiding sweets. Nevertheless, diet quality as assessed by the pro-healthy diet index (pHDI-10) and the Mediterranean Diet Score (MDS) showed a low intensity of consumption of pro-healthy food (average pHDI-10 value 31.4%; MDS 7.55/14 points, p = 0.0001). Women with endometriosis reported lower than recommended consumption of fruits, vegetables, dairy products and whole grains. A higher percentage of healthy controls had a “good” level of nutritional knowledge than women with endometriosis. The results of our study highlight the need to improve diet quality and nutritional knowledge among women with endometriosis as well as developing targeted dietary recommendations to help improve well-being and reduce the pain symptoms of endometriosis.
The influence of intracellular levels of cyclic nucleotides on cell proliferation and the induction of antibody synthesis

The intracellular ratio of adenosine 3',5'-cyclic monophosphate (cyclic AMP) to guanosine 3',5'-cyclic monophosphate (cyclic GMP) may control the developmental pathway followed by antibody-forming cell (AFC) precursors. The evidence for this is derived from several different types of experiments. First lipopolysaccharide (LPS) which is mitogenic for B lymphocytes, stimulates rapid, transient changes in intracellular levels of cyclic GMP but not cyclic AMP when added to mouse spleen cultures. Cyclic GMP itself stimulates DNA synthesis in these cultures, suggesting that the intracellular changes in cyclic GMP levels are involved in the mitogenic signal delivered by LPS to cells. The absolute amounts of cyclic nucleotides may vary widely in different cells under various conditions, however, the intracellular ratio of cyclic AMP to cyclic GMP is always high in nondividing cells and low in dividing cells. AFC precursors appear to respond to antigen in the absence of T-cell activity by inactivation (1-7). In the response to antigen in the presence of specific T cells, precursor cells proliferate and mature to AFC. Raising intracellular levels of cyclic AMP inhibits cell proliferation and leads to precursor cell inactivation (14, 15). It is suggested that the interaction of antigen with immunoglobulin receptors on the surface of precursors cells leads to the stimulation of adenylate cyclase activity and initiates the inactivation pathway. Since cyclic GMP stimulates immune responses in T- cell-depleted cultures (14, 15) and increasing cyclic GMP levels appear to be involved in the delivery of a mitogenic signal to cells, it is suggested that T-helper cells deliver a signal to precursor cells via the stimulation of guanylate cyclase to initiate the inductive pathway. It is suggested that it is the intracellular ratio of cyclic AMP to cyclic GMP that regulates the fate of precursor cells, not the absolute level of one cyclic nucleotide.
Rapid and extensive warming following cessation of solar radiation management

Solar radiation management (SRM) has been proposed as a means to alleviate the climate impacts of ongoing anthropogenic greenhouse gas (GHG) emissions. However, its efficacy depends on its indefinite maintenance, without interruption from a variety of possible sources, such as technological failure or global cooperation breakdown. Here, we consider the scenario in which SRM—via stratospheric aerosol injection—is terminated abruptly following an implementation period during which anthropogenic GHG emissions have continued. We show that upon cessation of SRM, an abrupt, spatially broad, and sustained warming over land occurs that is well outside 20th century climate variability bounds. Global mean precipitation also increases rapidly following cessation, however spatial patterns are less coherent than temperature, with almost half of land areas experiencing drying trends. We further show that the rate of warming—of critical importance for ecological and human systems—is principally controlled by background GHG levels. Thus, a risk of abrupt and dangerous warming is inherent to the large-scale implementation of SRM, and can be diminished only through concurrent strong reductions in anthropogenic GHG emissions.
Acoustic and Aerodynamic Effects of Interarticulator Timing in Voiceless Consonants

Interarticulator timing is a mechanism for producing linguistic contrasts that is widely used in different languages. This paper explores acoustic and aerodynamic effects of variations in laryngeal-oral coordination in voiceless consonants. Measurements of voice onset time and interarticulator phasing for individual tokens of stop consonants show weak correlations, indicating that interarticulator timing is only one factor determining voice onset time. Other factors most likely involved are glottal opening, transglottal pressure and air flow, and vocal fold tension. Taken together, these observations suggest that speakers may only have limited control of voice onset time. This could explain why languages do not seem to make fine-grain use of VOT for linguistic contrasts. Measurements of peak and minimum air flow during individual source pulses, obtained by inverse-filtering oral flow, show a pattern of decrease and increase in vowels following voiceless consonants. Subtle differences in the time course of these patterns occur following different consonants, suggesting that interarticulator phasing may be partly responsible for them. Closer examination reveals consistent correlations with interarticulator phasing for one speaker but inconsistent results for another. The results are discussed in terms of speech motor control and controlled variables in speech.
Characterization of canadian arctic fossil woods

Fossil woods from Axel Heiberg, Ellesmere, and Cornwallis islands in the Canadian Arctic were analyzed. Most specimens have been dated as Eocene, but one may be Paleocene, and as old as 65 million years. All the woods are gymnosperms. Based on recognizable characteristics, some of the Eocene specimens have been characterized as possibly spruce or larch, and one specimen has all the characteristics of Douglas fir. A Miocene wood has been identified as an especially well preserved white pine. Chemical and instrumental analyses showed that the Eocene and Paleocene specimens have undergone extensive carbohydrate degradation with nearly complete removal of hemicelluloses. The residual carbohydrate is mainly crystalline cellulose. The fossil woods are rich in lignin; some specimens contain over 80 per cent lignin. The fossil lignins are methoxyl-group deficient, sidechain degraded, and more condensed compared to recent softwood lignin. No evidence of bacterial or fungal decay was observed; hydrolysis was probably the major route of degradation.