Miracle-dz/UniFAR-FTUs huggingface dataset:
Mass Spectrometry Analysis of Hepcidin Peptides in Experimental Mouse Models

The mouse is a valuable model for unravelling the role of hepcidin in iron homeostasis, however, such studies still report hepcidin mRNA levels as a surrogate marker for bioactive hepcidin in its pivotal function to block ferroportin-mediated iron transport. Here, we aimed to assess bioactive mouse Hepcidin-1 (Hep-1) and its paralogue Hepcidin-2 (Hep-2) at the peptide level. To this purpose, fourier transform ion cyclotron resonance (FTICR) and tandem-MS was used for hepcidin identification, after which a time-of-flight (TOF) MS-based methodology was exploited to routinely determine Hep-1 and -2 levels in mouse serum and urine. This method was biologically validated by hepcidin assessment in: i) 3 mouse strains (C57Bl/6; DBA/2 and BABL/c) upon stimulation with intravenous iron and LPS, ii) homozygous Hfe knock out, homozygous transferrin receptor 2 (Y245X) mutated mice and double affected mice, and iii) mice treated with a sublethal hepatotoxic dose of paracetamol. The results showed that detection of Hep-1 was restricted to serum, whereas Hep-2 and its presumed isoforms were predominantly present in urine. Elevations in serum Hep-1 and urine Hep-2 upon intravenous iron or LPS were only moderate and varied considerably between mouse strains. Serum Hep-1 was decreased in all three hemochromatosis models, being lowest in the double affected mice. Serum Hep-1 levels correlated with liver hepcidin-1 gene expression, while acute liver damage by paracetamol depleted Hep-1 from serum. Furthermore, serum Hep-1 appeared to be an excellent indicator of splenic iron accumulation. In conclusion, Hep-1 and Hep-2 peptide responses in experimental mouse agree with the known biology of hepcidin mRNA regulators, and their measurement can now be implemented in experimental mouse models to provide novel insights in post-transcriptional regulation, hepcidin function, and kinetics.
HOW ADMIXTURES AFFECT SHRINKAGE AND CREEP

This article summarizes the findings of a detailed review of experimental test data on elasticity, creep, and shrinkage of concrete containing chemical and mineral admixtures presented at the ACI Fall Convention at Atlanta in 1997. The main purpose of this review was to assess any common effects on deformation of these types of the admixtures: plasticizers (water reducers), superplasticizers (high-range water reducers), blast furnace slag, fly ashes, and silica fume.
Requirement of a Vasodilator-stimulated Phosphoprotein Family Member for Cell Adhesion, the Formation of Filopodia, and Chemotaxis in Dictyostelium* 210

We have examined the function of a member of the vasodilator-stimulated phosphoprotein family of proteins (DdVASP) inDictyostelium. Ddvasp null cells lack filopodia, whereas targeting DdVASP to the plasma membrane with a myristoyl tag results in a significant increase in filopodia. The proline-rich domain-Ena/VASP homology 2 structure is required for both actin polymerization activity and filopodia formation.Ddvasp null cells exhibit a chemotaxis defect, which appears to be due to a defect in the ability of the cells to properly adhere to the substratum and to suppress lateral pseudopod extension. We demonstrate that during chemotaxis, the anterior ∼50% of the cell lifts from the substratum and remains elevated for up to 1 min. These defects lead to a significant decrease in chemotaxis efficiency. DdVASP localizes to the leading edge in migrating cells and to the tips of filopodia. In addition, Ddvasp null cells have a defect in particle adhesion but internalize particles normally. Our results provide new insights into the function of DdVASP in controlling the actin cytoskeleton during chemotaxis and filopodia formation.
An efficient procedure for computing lightning induced overvoltages on overhead lines

Overvoltages on electrical power or telecommunications overhead lines due to nearby lightning strokes can cause faults and disturbances in electrical and electronic devices and apparatuses connected to them. It is important to understand these induced overvoltages, because equipments with electronic devices are sensitive to such disturbances. In this paper, lightning radiated electromagnetic fields caused by the return stroke are evaluated with transmission line engineering models, representing the current through the lightning channel. Integral equations are solved by means the Trapezoidal Rule, resulting in an efficient way to compute the radiated electromagnetic field. Also, the coupling between the electromagnetic field radiated by the return stroke and the overhead line is considered by using The Method of Characteristics. In this way, transmission line equations to describe voltages and currents in the overhead line are solved in order to compute the induced overvoltages. Results are compared with others internationally published.
Efficacy of Cytokine Removal by Plasmadiafiltration Using a Selective Plasma Separator: In Vitro Sepsis Model

More effective removal of pro‐ and anti‐inflammatory cytokines may play an important role in the treatment of sepsis. Plasmadiafiltration (PDF) with a larger selective plasma separator was performed to study the cytokine and plasma protein permeability profiles of the membrane in an in vitro sepsis model. The in vitro sepsis model was constructed by exposure of human whole blood to bacterial lipopolysaccharide. EVACURE 2A, a selective plasma separator, was placed in the blood circuit of PDF. Sieving coefficients of cytokines and plasma protein were tested in post‐dilution PDF mode at the following operating parameters: blood flow rate 150 mL/min; dialysate flow rate 33.33 mL/min; replacing fluid flow rate 6.67 mL/min; ultrafiltration rate 5 mL/min. An enzyme linked immunoadsorbent assay was used to measure the concentrations of tumor necrosis factor‐α (TNF‐α), high‐mobility group box 1 protein (HMGB1), interleukin‐1β (IL‐1β), interleukin‐1 receptor antagonist (IL‐1ra), interleukin‐2 (IL‐2), interleukin‐2 receptor (IL‐2r), interleukin‐6 (IL‐6), interleukin‐8 (IL‐8), interleukin‐10 (IL‐10) in plasma and ultrafiltrate. Sieving coefficients of different solutes ranged from 0.1 to 1.0 at first, decreased 10%–60% after 1 h of PDF, and then remained stable. Total clearance rates of cytokines ranged from 15 to 80 mL/min. The concentrations of cytokines decreased 20–80% after 1 hour of PDF. The sieving coefficient of albumin was 0.1 at first and then decreased to 0.05 after 1 hour of therapy. Plasmadiafiltration with Evacure 2A plasma separator can effectively remove almost all of the inflammatory mediators with low albumin loss.
dipeptide motifs of the p 48 subunit of chromatin assembly factor-1 is essential for viability of DT 40 chicken B cell line

Chromatin assembly factor-1 (CAF-1) is involved in chromatin assembly and comprises three subunits, p48, p60 and p150. The p48 subunit, CAF-1p48 (p48), with seven WD (Trp-Asp) repeat motifs, is a member of the WD protein family. The immunoprecipitation experiment revealed that ßpropeller structure of p48 was less stringent for its binding to HDAC1, but more stringent for its binding to both histone H4 and p60, indicating that the proper ß-propeller structure of p48 is essential for the binding to these two proteins. Complementation experiments, involving missense and truncated mutants of FLAG-tagged p48, revealed that mutations of every of seven WD dipeptide motifs, like both the N-terminal and C-terminal truncated mutations, could not rescue for the tetinduced lethality. These results indicate not only that p48 is essential for the viability of vertebrate cells, although the yeast p48 homolog is nonessential, but also that all the seven WD dipeptide motifs are necessary for the maintenance of the proper structure of p48 that is fundamentally important for its functions.
The effect of vertical air gaps to thermal transmittance of horizontal thermal insulating layer

Abstract In order to reduce the amounts of work at the construction site, single‐ply dual density thermal insulating roofing boards are used with increasing frequency for thermal insulation of flat roofs. In this case, the joints between boards are not overlapped by the other ply over it; therefore gaps of varying width form between the sides of the boards through the entire thickness of the insulating layer, whose effect on the effective thermal conductivity of the thermal insulating layer must be evaluated. The aim of this project was to assess the reliability of standard method, used to determine the impact of such air gaps on the effective thermal conductivity of the thermal insulating layer by comparing the results of calculations and the results of measurements of thermal conductivity, also to determine the correction factors for thermal transmittance of horizontal thermal insulation layers due to the forming vertical air gaps between the single‐ply mineral wool boards. After measurements of thermal.
Cooperative interference mitigation and handover management for heterogeneous cloud small cell networks

Heterogeneous small cell networks have attracted much attention for satisfying users' explosive data traffic requirements. The heterogeneous cloud small cell network (HCSNet), which combines cloud computing and a heterogeneous small cell network, will likely play an important role in 5G mobile communication networks. However, with massive deployment of small cells, co-channel interference and handover management are two important problems in an HCSNet, especially for cell edge users. In this article, we examine the problems of cooperative interference mitigation and handover management in an HCSNet. A network architecture is described to combine cloud radio access network with small cells. An effective CoMP clustering scheme using affinity propagation is adopted to mitigate cell edge users' interference. A low-complexity handover management scheme is presented, and its signaling procedure is analyzed in an HCSNet. Numerical results show that with the proposed network architecture, CoMP clustering and handover management schemes can significantly increase the capacity of HCSNet while maintaining users' quality of service.
New parameters for identifying subclinical atherosclerosis in patients with primary Sjögren's syndrome: a pilot study.

OBJECTIVES We investigated sub-clinical cardiovascular involvement in primary Sjögren's syndrome (pSS) patients by means of ADMA, coronary flow reserve (CFR), intima media thickness (cIMT), pulse wave velocity (PWV) and myocardial deformation. METHODS The study involved 22 outpatients with pSS (6 males, 16 females; mean age 60.14±7.81 years) and no documentable cardiovascular disease, and 22 age- and gender-matched controls. Dipyridamole transthoracic stress echocardiography was used to evaluate wall motion and CFR. A CFR value of <2.5 was considered a sign of impaired coronary function. We also evaluated cIMT arterial stiffness PWV and plasma ADMA levels, and made a speckle tracking echocardiography (STE) analysis. RESULTS All of the patients were affected by pSS. Although within the normal range, the patients' CFR was lower than that of the controls (median 2.70; IQR 2.40-2.90 vs. 3.20; IQR 3.06-3.33; p<0.0001), whereas their ADMA levels were significantly higher (median 0.81 μM; IQR 0.79-0.85 μM vs. 0.54 μM; IQR 0.52-0.58 μM; p<0.0001). Both left and right PWV values were significantly higher in the patients than in the controls (median 8.8 m/s right and 8.9 m/s left vs. 6.86 and 6.89 m/s), whereas QIMT was substantially similar in the two groups. CONCLUSIONS Higher ADMA levels suggest the presence of endothelial dysfunction and sub-clinical atherosclerosis in pSS patients, even in the case of a normal CFR. This finding is supported by the PWV values, which were higher in the pSS patients. ADMA levels and PWV values may be useful markers for identifying early endothelial dysfunction in pSS patients.
Automated Verification of Group Key Agreement Protocols

We advance the state-of-the-art in automated symbolic cryptographic protocol analysis by providing the first algorithm that can handle Diffie-Hellman exponentiation, bilinear pairing, and AC-operators. Our support for AC-operators enables protocol specifications to use multisets, natural numbers, and finite maps. We implement the algorithm in the TAMARIN prover and provide the first symbolic correctness proofs for group key agreement protocols that use Diffie-Hellman or bilinear pairing, loops, and recursion, while at the same time supporting advanced security properties, such as perfect forward secrecy and eCK-security. We automatically verify a set of protocols, including the STR, group Joux, and GDH protocols, thereby demonstrating the effectiveness of our approach.
Radiotherapy for Adrenal Metastasis from Hepatocellular Carcinoma: A Multi-Institutional Retrospective Study (KROG 13-05)

Although the adrenal glands are not common sites of metastasis from hepatocellular carcinoma (HCC), this metastasis can be met in patients with advanced HCC in some clinical settings. However, the effectiveness of radiotherapy against such metastases is unclear. Therefore, we performed the present multi-institutional study to investigate tumor response, overall survival (OS), treatment-related toxicity, and prognostic factors after radiotherapy. We retrospectively reviewed 134 patients who completed a planned radiotherapy for their adrenal metastases. Complete response was noted in 6 (4.3%), partial response in 48 (34.0%), and stable disease in 78 patients (55.3%). The median OS was 12.8 months, and the 1-, 2-, and 5-year OS rates were 53.1%, 23.9%, and 9.3%, respectively. Grade 3 anorexia occurred in 2 patients, grade 3 diarrhea in 1, and grade 3 fatigue in 1. Multivariate analyses revealed that the following factors had significant effects on OS: controlled intrahepatic tumor; controlled extrahepatic metastasis; and Child-Pugh class A. Although patients with adrenal metastasis from HCC had poor OS, radiotherapy provided an objective response rate of 38.3% and disease stability of 93.6%, with minimal adverse events. Therefore, radiotherapy for these patients could represent a good treatment modality, especially for patients with controlled intrahepatic tumors, controlled extrahepatic metastasis, and good hepatic function.
SimRank: A link analysis based blogger recommendation algorithm using text similarity

Blogs have influenced our life profoundly and people preferred to subscribe to influential bloggers they are interested in. Hence, the identification of influential bloggers automatically has become an important task. Previous researches focus on the blog sites in which there exist abundant hyperlinks, but their methods can not scale to ones that have few hyperlinks. In this paper, we propose a novel algorithm called SimRank to recommend influential bloggers. Our algorithm is based on the observation that the reproduction of blog posts and similar contents is common in blogosphere, which form implicit links between bloggers. By measuring the text similarity of the blog posts, we create link graph between bloggers, and adopt the PageRank algorithm to rank the importance of bloggers. Experimental results indicate that our proposed algorithm is effective in recommending bloggers.
Radial Flow Permeability Testing of an Argillaceous Limestone

Argillaceous Lindsay limestone is the geologic storage formation that will be encountered at the site for the construction of a deep ground repository in Ontario, Canada, for the storage of low to intermediate level nuclear waste. The permeability of the Lindsay limestone is a key parameter that will influence the long‐term movement of radionuclides from the repository to the geosphere. This paper describes the use of both steady‐state and transient radial flow laboratory tests to determine the permeability of this argillaceous limestone. The interpretation of the tests is carried out using both analytical results and computational models of flow problems that exhibit radial symmetry. The results obtained from this research investigation are compared with the data available in the literature for similar argillaceous limestones mainly found in the Lindsay (Cobourg) formation. The experiments give permeabilities in the range of 1.0 × 10−22 to 1.68 × 10−19 m2 for radial flows that are oriented along bedding planes under zero axial stress. The factors influencing transient pulse tests in particular and the interpretation of the results are discussed.
Naturally Occurring Alpha-Synuclein Autoantibodies in Parkinson’s Disease: Sources of (Error) Variance in Biomarker Assays

Alpha-synuclein (α-Syn) plays a pivotal role in the pathophysiology of Parkinson’s disease (PD), which can partly be modulated by innate and adaptive immune functions, and vice versa. Here, naturally occurring α-Syn autoantibodies (α-Syn-nAbs) may be effective against α-Syn pathoetiology and may serve as a PD biomarker. However, serum and cerebrospinal fluid α-Syn-nAbs levels still lack consistent evidence as required for a reliable PD biomarker. Serum and cerebrospinal fluid α-Syn-nAbs levels of 66 PD patients and 69 healthy controls were assessed using a validated ELISA assay. Moreover, potential sources of error variance including unspecific ELISA background signals, free serum hemoglobin concentrations, α-Syn plate coating procedures, and differences in α-Syn-nAbs standards, were investigated. PD patients and controls did not differ in serum (p = .49) nor cerebrospinal fluid (p = .29) α-Syn-nAbs levels. Interestingly, free serum hemoglobin concentrations were negatively correlated with α-Syn-nAbs levels in controls (Spearman = −.41, p<.001), but not in PD patients ( = .16, p = .21). ELISA α-Syn plate coating procedures impacted inter-assay variability (same day coating: 8–16%; coating on different days: 16–58%). α-Syn-nAbs standards from different purification batches differed regarding optical density measured in ELISAs suggesting differences in α-Syn affinity. While α-Syn-nAbs levels may represent a potential PD biomarker, several methodological issues have to be considered to increase reproducibility of α-Syn-nAbs findings. Further studies using standardized protocols minimizing sources of error variance may be necessary to establish a reliable PD α-Syn-nAbs biomarker.
Neuroimaging self-esteem: a fMRI study of individual differences in women

Although neuroimaging studies strongly implicate the medial prefrontal cortex (ventral and dorsal), cingulate gyrus (anterior and posterior), precuneus and temporoparietal cortex in mediating self-referential processing (SRP), little is known about the neural bases mediating individual differences in valenced SRP, that is, processes intrinsic to self-esteem. This study investigated the neural correlates of experimentally engendered valenced SRP via the Visual–Verbal Self-Other Referential Processing Task in 20 women with fMRI. Participants viewed pictures of themselves or unknown other women during separate trials while covertly rehearsing ‘I am’ or ‘She is’, followed by reading valenced trait adjectives, thus variably associating the self/other with positivity/negativity. Response within dorsal and ventral medial prefrontal cortex, cingulate cortex and left temporoparietal cortex varied with individual differences in both pre-task rated self-descriptiveness of the words, as well as task-induced affective responses. Results are discussed as they relate to a social cognitive and affective neuroscience view of self-esteem.
Perception of self-generated action in schizophrenia

Introduction. Self-generated actions involve central processes of sensorimotor integration that continuously monitor sensory inputs to ensure that motor outputs are congruent with our intentions. This mechanism works automatically in normal conditions but becomes conscious whenever a mismatch happens during the execution of action between expected and current sensorimotor reafferences. It is now admitted in the literature that sensorimotor processes as well as the ability to predict the consequences of our own actions imply the existence of a forward model of action, which is based on efference copies. Recently, it has been proposed that positive symptoms expressed by schizophrenic patients, such as delusions of control or thought insertions, arise because of a deficiency in this forward model, and more particularly, because of a lack of awareness of certain aspects of motor control derived from such an internal model. Method. To test further this hypothesis, 19 schizophrenic patients (10 with and 9 without Schneiderian symptoms) and 19 control subjects performed a visuo-motor conflict task and had verbally to report the felt position of their hand at the end of each trial. Results. Under this experimental procedure, schizophrenic patients - whatever their clinical phenotype - failed to switch to a conscious representation of their hand movements, and then consequently to maintain their level of performance for the sensorimotor adjustment in comparison with controls. Conclusion. Our findings suggest two facts. First, that a functional monitoring of action, based on a forward
Integral point sets over finite fields

We consider point sets in the affine plane Fq where each Euclidean distance of two points is an element of Fq . These sets are called integral point sets and were originally defined in m-dimensional Euclidean spaces Em. We determine their maximal cardinality I(Fq , 2). For arbitrary commutative rings R instead of Fq or for further restrictions as no three points on a line or no four points on a circle we give partial results. Additionally we study the geometric structure of the examples with maximum cardinality.
Isolation of a human hepatic ferritin receptor

A human hepatic ferritin receptor has been isolated from human liver and has been purified using affinity chromatography. An affinity constant of 6.0 × 108 moles−1 liter was determined for the ferritin receptor. The molecular weight was estimated to be approximately 53,000 by gel electrophoresis. Binding of ferritin to the receptor coupled to a microparticulate support was specific for human liver ferritin with no binding of rat or porcine ferritin. Binding was unaffected by a 100‐fold excess of human transferrin, human asialoo‐rosomucoid and bovine albumin. After treatment of the receptor protein with trypsin, binding was not detected. The human hepatic ferritin receptor may play an important role in the uptake of iron into the hepatocyte in physiological and pathological conditions.
Facebook Addiction Among Turkish College Students: The Role of Psychological Health, Demographic, and Usage Characteristics

This study explored Facebook addiction among Turkish college students and its behavioral, demographic, and psychological health predictors. The Facebook Addiction Scale (FAS) was developed and its construct validity was assessed through factor analyses. A total of 447 students reported their personal information and Facebook usage and completed the FAS and General Health Questionnaire (GHQ-28). The results revealed that weekly time commitment, social motives, severe depression, and anxiety and insomnia positively predicted Facebook addiction. Neither demographic variables nor the interactions of gender by usage characteristics were found to be significant predictors.
A meta-analytic review of research on hostility and physical health.

A new meta-analysis of research on hostility and physical health was conducted that includes 15 studies used in previous meta-analytic reviews and 30 new independent studies. Overall, the results suggest that hostility is an independent risk factor for coronary heart disease (CHD). For structured interview indicators of potential for hostility, the weighted mean r was . 18. After controlling for other risk factors for CHD, the widely used Cook-Medley Hostility Scale and other cognitive-experiential measures were most predictive of all-cause mortality (weighted mean r = .16) and, to a lesser extent, CHD (weighted mean r = .08). Similar to other areas of research, the increased use of high-risk studies in recent years produced an increase in null findings.