Miracle-dz/UniFAR-FTUs huggingface dataset:
Variable Temperature Stress in the Nematode Caenorhabditis elegans (Maupas) and Its Implications for Sensitivity to an Additional Chemical Stressor

A wealth of studies has investigated how chemical sensitivity is affected by temperature, however, almost always under different constant rather than more realistic fluctuating regimes. Here we compared how the nematode Caenorhabditis elegans responds to copper at constant temperatures (8–24°C) and under fluctuation conditions of low (±4°C) and high (±8°C) amplitude (averages of 12, 16, 20°C and 16°C respectively). The DEBkiss model was used to interpret effects on energy budgets. Increasing constant temperature from 12–24°C reduced time to first egg, life-span and population growth rates consistent with temperature driven metabolic rate change. Responses at 8°C did not, however, accord with this pattern (including a deviation from the Temperature Size Rule), identifying a cold stress effect. High amplitude variation and low amplitude variation around a mean temperature of 12°C impacted reproduction and body size compared to nematodes kept at the matching average constant temperatures. Copper exposure affected reproduction, body size and life-span and consequently population growth. Sensitivity to copper (EC50 values), was similar at intermediate temperatures (12, 16, 20°C) and higher at 24°C and especially the innately stressful 8°C condition. Temperature variation did not increase copper sensitivity. Indeed under variable conditions including time at the stressful 8°C condition, sensitivity was reduced. DEBkiss identified increased maintenance costs and increased assimilation as possible mechanisms for cold and higher copper concentration effects. Model analysis of combined variable temperature effects, however, demonstrated no additional joint stressor response. Hence, concerns that exposure to temperature fluctuations may sensitise species to co-stressor effects seem unfounded in this case.
Purification and Characterization of Human Adrenomedullin Binding Protein-1

We recently discovered that the vascular responsiveness to adrenomedullin (AM), a potent vasoactive peptide, decreased during sepsis and hemorrhage in the rat and was markedly improved by its novel binding protein (AMBP-1). Moreover, AM/AMBP-1 appears to be one of the leading candidates for further development to treat sepsis and hemorrhage. However, the extremely high cost of commercial AMBP-1 limits the development of human AM and AMBP-1 as therapeutic agents. The purpose of this study was to isolate and purify AMBP-1 from normal human serum and test its stability and biological activity under in vitro and in vivo conditions. AMBP-1 was isolated and purified from normal human serum with a yield of about 3.0 mg per 100 mL and purity of >99%. The purified AMBP-1 has a AM-binding capacity similar to that of the commercial AMBP-1. Human AM and human AMBP-1 in combination significantly inhibited lipopolysaccharide-induced tumor necrosis factor (TNF)-α and interleukin (IL)-6 production from macrophages. The biological activity of the purified human AMBP-1 was well preserved when stored at 45°C for 5 d in solution or at 100°C for 1 h in powder. Moreover, administration of AM and purified AMBP-1 to hemorrhaged rats attenuated tissue injury and neutrophil accumulation. Purified AMBP-1 in combination with AM also suppressed the hemorrhage-induced rise in serum cytokines TNF-α and IL-6. Thus, we have successfully purified biologically active AMBP-1 from human normal serum and demonstrated the stability of purified human AMBP-1. This technique will enable us to further develop human AM/AMBP-1 as a novel treatment for safe and effective therapy of patients with hemorrhagic shock, sepsis, and ischemic injury.
Pharmacokinetics of Compounded Intravenous and Oral Gabapentin in Hispaniolan Amazon Parrots (Amazona ventralis)

Abstract: Neuropathic pain is a manifestation of chronic pain that arises with damage to the somatosensory system. Pharmacologic treatment recommendations for alleviation of neuropathic pain are often multimodal, and the few reports communicating treatment of suspected neuropathic pain in avian patients describe the use of gabapentin as part of the therapeutic regimen. To determine the pharmacokinetics of gabapentin in Hispaniolan Amazon parrots (Amazona ventralis), compounded gabapentin suspensions were administered at 30 mg/kg IV to 2 birds, 10 mg/kg PO to 3 birds, and 30 mg/kg PO to 3 birds. Blood samples were collected immediately before and at 9 different time points after drug administration. Plasma samples were analyzed for gabapentin concentration, and pharmacokinetic parameters were calculated with both a nonlinear mixed-effect approach and a noncompartmental analysis. The best compartmental, oral model was used to simulate the concentration-time profiles resulting from different dosing scenarios. Mild sedation was observed in both study birds after intravenous injection. Computer simulation of different dosing scenarios with the mean parameter estimates showed that 15 mg/kg every 8 hours would be a starting point for oral dosing in Hispaniolan Amazon parrots based on effective plasma concentrations reported for human patients; however, additional studies need to be performed to establish a therapeutic dose.
Simultaneous Extratympanic Electrocochleography and Auditory Brainstem Responses Revisited

The purpose of this study was to revisit the two-channel, simultaneous click-evoked extratympanic electrocochleography and auditory brainstem response (ECoG/ABR) recording technique for clinical use in normal hearing participants. Recording the compound action potential (AP) of the ECoG simultaneously with ABR may be useful when Wave I of the ABR is small or diminished in patients with sensorineural or retrocochlear disorder and minimizes overall test time. In contrast to some previous studies that used the extratympanic electrode both as non-inverting electrode for the ECoG and inverting electrode for ABR, this study maintained separate recording channel montages unique to conventional click-evoked ECoG and ABR recordings. That is, the ABR was recorded using a vertical channel (Cz to ipsilateral earlobe), while the ECoG with custom extratympanic electrode was recorded using a horizontal channel (tympanic membrane to contralateral earlobe). The extratympanic electrode is easy to fabricate in-house, or can be purchased commercially. Maintaining the conventional ABR montage permits continued use of traditional normative data. Broadband clicks at a fixed level of 85 dB nHL were presented with alternating polarity at stimulus rates of 9.3, 11.3, and 15.3/s. Different stimulation rates were explored to identify the most efficient rate without sacrificing time or waveform morphology. Results revealed larger ECoG AP than ABR Wave I, as expected, and no significant difference across stimulation rate and no interaction effect. Extratympanic electrode placement takes little additional clinic time and may improve the neurodiagnostic utility of the ABR.
A new proposal for radical hysterectomy.

OBJECTIVE The authors revised the surgical procedure for radical hysterectomy utilizing detailed observation of the venous system, connective fascial sheets, and neural pathways within the uterine supports. STUDY DESIGN The anterior, middle, and posterior uterine supports were reclassified into two systems, supporting or fascial and drainage or areoral. Supporting system consisted of the superficial layer of the vesicouterine ligament, fascial part of the Mackenrodt ligament, sacrouterine ligament, and rectovaginal ligament, whereas drainage system consisted of the deep layer of the vesicouterine ligament, vascular part of the Mackenrodt ligament, and the so-called mesoureter. The operative procedure was planned according to the continuity of these ligaments and executed first by excising the fascia and then dissecting the denuded areoral tissue. RESULTS Among the 15 patients who underwent surgery for uterine cancer during a 2-year period the mean (SD) time required for the operation was 305.5 +/- 30.5 min and the mean (SD) total volume of blood loss 592.0 +/- 238.2 ml. A mean (SD) period of 14.3 +/- 3.8 postoperative days was required until the volume of the residual urine decreased to less than 50 mL. CONCLUSION The present operation has been structured more three-dimensionally and systematically than before. Further, safety of the operation was significantly improved including prevention of hemorrhage and preservation of bladder function.
Ontology learning from Italian legal texts

The paper reports on the methodology and preliminary results of a case study in automatically extracting ontological knowledge from Italian legislative texts. We use a fully--implemented ontology learning system (T2K) that includes a battery of tools for Natural Language Processing (NLP), statistical text analysis and machine language learning. Tools are dynamically integrated to provide an incremental representation of the content of vast repositories of unstructured documents. Evaluated results, however preliminary, show the great potential of NLP--powered incremental systems like T2K for accurate large--scale semi--automatic extraction of legal ontologies.
Physical activity interacts with adiposity in determining cardiometabolic risk in adolescents.

BACKGROUND Moderate-to-vigorous physical activity (MVPA) has been negatively associated with cardiometabolic risk. We sought to determine if MVPA interacts with body-mass index (BMI) and waist circumference (WC) in determining cardiometabolic risk in adolescents. METHODS This cross-sectional study included cardiometabolic risk (blood pressure [BP], nonfasting lipids) screening and a 7-day recall physical activity questionnaire in 4,104 adolescents (51% male; mean age: 14.6 ± 0.5 years old). WC- and BMI- percentiles were used to define anthropometric categories (including obese adolescents: 90th WC, 85th BMI). RESULTS Obesity in adolescents was associated with lower levels of high-density lipoprotein (HDL cholesterol (Estimate [EST]: -0.28(0.07) mmol/L, p < . 001) and higher non-HDL cholesterol (EST: +0.38(0.14) mmol/L, p = . 008). Each additional day with 20 min of MVPA was associated with lower non-HDL cholesterol (EST: -0.014(0.005) mmol/L/days/week, p = . 003), independent of anthropometric category. Each additional day with 20 min of MVPA was associated with an increased odds ratio (OR) for higher BP category in obese adolescents (OR: 1.055, 95% CI: 1.028-1.084, p < .001) and a lower odds ratio for higher BP category in presumably-muscular adolescents (OR: 0.968, 95% CI: 0.934-0.989, p = .005). CONCLUSIONS An increase in MVPA was associated with an increased likelihood for higher BP category in obese adolescents. The dose-response relationship between physical activity and cardiometabolic risk needs to be evaluated in adolescents of varying anthropometry categories.
Failure Prediction Technique for Compression Loaded Carbon Fibre-Epoxy Laminate with Open Holes

The inplane compressive fracture behaviour of a carbon fibre-epoxy laminate containing a single hole is examined. Failure is due to 0° fibre microbuckling surrounded by delamination. The microbuckled zone initiates at the hole boundary. It extends stably under increasing load before becoming unstable at a critical microbuckle length of 2–3 mm. The damage propagation and failure of the orthotropic laminate is analysed by a cohesive zone model, in which damage around the open hole is represented by a line crack loaded on its faces. Good agreement between experimental and predicted notched strength is observed for the chosen material and lay-up.
Articulation Rate Filtering of CQCC Features for Automatic Speaker Verification

This paper introduces a new articulation rate filter and reports its combination with recently proposed constant Q cepstral coefficients (CQCCs) in their first application to automatic speaker verification (ASV). CQCC features are extracted with the constant Q transform (CQT), a perceptually-inspired alternative to Fourier-based approaches to time-frequency analysis. The CQT offers greater frequency resolution at lower frequencies and greater time resolution at higher frequencies. When coupled with cepstral analysis and the new articulation rate filter, the resulting CQCC features are readily modelled using conventional techniques. A comparative assessment of CQCCs and mel frequency cepstral coefficients (MFCC) for a short-duration speaker verification scenario shows that CQCCs generally outperform MFCCs and that the two feature representations are highly complementary; fusion experiments with the RSR2015 and RedDots databases show relative reductions in equal error rates of as much as 60% compared to an MFCC baseline.
A leftover service curve approach to analyze demultiplexing in queueing networks

Queueing networks are typically subject to demultiplexing operations, whereby network nodes split flows into multiple sub-flows. The demultiplexing operation captures relevant network aspects such as packet loss or multi-path routing. In this paper we propose a novel approach to analyze queueing networks with demultiplexing. The basic idea is to represent a network node implementing a demultiplexing operation on an output flow as an equivalent system for which the corresponding input flow is logically demultiplexed according to the demultiplexing operation at the output. In this way, the service given to one of the demultiplexed sub-flows at the output can be expressed in terms of a leftover service curve, and consequently performance bounds can be derived using the network calculus methodology. Using numerical illustrations, we show that the obtained bounds improve upon existing bounds, especially in the case of the rather small sub-flows.
Determination of Thermal Properties and Morphology of Eucalyptus Wood Residue Filled High Density Polyethylene Composites

Thermal behaviors of eucalyptus wood residue (EWR) filled recycled high density polyethylene (HDPE) composites have been measured applying the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Morphology of the materials was also studied using scanning electron microscope (SEM). Addition of the EWR into the recycled HDPE matrix reduced the starting of degradation temperature. EWR filled recycled HDPE had two main decomposition peaks, one for EWR around 350 °C and one for recycled HDPE around 460 °C. Addition of EWR did not affect the melting temperature of the recycled HDPE. Morphological study showed that addition of coupling agent improved the compatibility between wood residue and recycled HDPE.
Black Hole Mass Estimates from Reverberation Mapping and from Spatially Resolved Kinematics

Black hole (BH) masses that have been measured by reverberation mapping in active galaxies fall significantly below the correlation between bulge luminosity and BH mass determined from spatially resolved kinematics of nearby normal galaxies. This discrepancy has created concern that one or both techniques suffer from systematic errors. We show that BH masses from reverberation mapping are consistent with the recently discovered relationship between BH mass and galaxy velocity dispersion. Therefore, the bulge luminosities are the probable source of the disagreement, not problems with either method of mass measurement. This result underscores the utility of the BH mass-velocity dispersion relationship. Reverberation mapping can now be applied with increased confidence to galaxies whose active nuclei are too bright or whose distances are too large for BH searches based on spatially resolved kinematics.
Preparatory Activations across a Distributed Cortical Network Determine Production of Express Saccades in Humans

Reaction time variability across trials to identical stimuli may arise from both ongoing and transient neural processes occurring before trial onset. These processes were examined with dense-array EEG as humans completed saccades in a “gap” paradigm known to elicit bimodal variability in response times, including separate populations of “express” and regular reaction time saccades. Results indicated that express reaction time trials could be differentiated from regular reaction time trials by (1) pretrial phase synchrony of occipital cortex oscillations in the 8–9 Hz (low alpha) frequency range (lower phase synchrony preceding express trials), (2) subsequent mid- and late-gap period cortical activities across a distributed occipital-parietal network (stronger activations preceding express trials), and (3) posttarget parietal activations locked to response generation (weaker preceding express trials). A post hoc path analysis suggested that the observed cortical activations leading to express saccades are best understood as an interdependent chain of events that affect express saccade production. These results highlight the importance of a distributed posterior cortical network, particularly in right hemisphere, that prepares the saccade system for rapid responding.
Obesity and Breast Cancer: Current Insights on the Role of Fatty Acids and Lipid Metabolism in Promoting Breast Cancer Growth and Progression

Obesity and excess accumulation of adipose tissue are known risk factors for several types of cancer, including breast cancer. With the incidence of obesity constantly rising worldwide, understanding the molecular details of the interaction between adipose tissue and breast tumors, the most common tumors in women, becomes an urgent task. In terms of lipid metabolism, most of the studies conducted so far focused on upregulated de novo lipid synthesis in cancer cells. More recently, the use of extracellular lipids as source of energy came into focus. Especially in obesity, associated dysfunctional adipose tissue releases increased amounts of fatty acids, but also dietary lipids can be involved in promoting tumor growth and progression. In addition, it was shown that breast cancer cells and adipocytes, which are a major component of the stroma of breast tumors, are able to directly interact with each other. Breast cancer cells and adjacent adipocytes exchange molecules such as growth factors, chemokines, and interleukins in a reciprocal manner. Moreover, it was shown that breast cancer cells can access and utilize fatty acids produced by neighboring adipocytes. Thus adipocytes, and especially hypertrophic adipocytes, can act as providers of lipids, which can be used as a source of energy for fatty acid oxidation and as building blocks for tumor cell growth.
Tracing Black Hole Mergers Through Radio Lobe Morphology

Binary supermassive black holes are produced by galactic mergers as the black holes from the two galaxies fall to the center of the merged system and form a bound pair. The two black holes will eventually coalesce in an enormous burst of gravitational radiation. Here we show that the orientation of a black hole's spin axis would change dramatically even in a minor merger, leading to a sudden flip in the direction of any associated jet. We identify the winged or X-type radio sources with galaxies in which this has occurred. The inferred coalescence rate is similar to the overall galaxy merger rate, implying that of the order of one merger event per year could be detected by gravitational wave interferometers.
Arabidopsis Basic Leucine Zipper Proteins That Mediate Stress-Responsive Abscisic Acid Signaling Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010362.

The phytohormone abscisic acid (ABA) plays an essential role in adaptive stress responses. The hormone regulates, among others, the expression of numerous stress-responsive genes. From various promoter analyses, ABA-responsive elements (ABREs) have been determined and a number of ABRE binding factors have been isolated, although their in vivo roles are not known. Here we report that the ABRE binding factors ABF3 and ABF4 function in ABA signaling. The constitutive overexpression of ABF3 or ABF4 in Arabidopsis resulted in ABA hypersensitivity and other ABA-associated phenotypes. In addition, the transgenic plants exhibited reduced transpiration and enhanced drought tolerance. At the molecular level, altered expression of ABA/stress-regulated genes was observed. Furthermore, the temporal and spatial expression patterns of ABF3 and ABF4 were consistent with their suggested roles. Thus, our results provide strong in vivo evidence that ABF3 and ABF4 mediate stress-responsive ABA signaling.
Simulating the effects of climate change on fire regimes in Arctic biomes: implications for caribou and moose habitat

Wildfire is the primary ecological driver of succession in the boreal forest and may become increasingly important within tundra ecosystems as the Arctic warms. Migratory barren-ground caribou (Rangifer tarandus granti) rely heavily on terricolous lichens to sustain them through the winter months. Lichens preferred by caribou can take 50 or more years to recover after being consumed by wildfires. We simulated effects of climate change on the fire regime within the winter range of one of the largest caribou herds in the world, the Western Arctic Herd, to assess how their forage may be impacted. We forecast that the total area burned (AB) in the near term (2008–2053) will be 0–30% greater than during our historic reference period (1950–2007) depending on the climate model (CGCM3.1 or ECHAM5) considered. Further into the future (i.e., 2054–2099), we forecast AB to increase 25–53% more than during our reference period. In contrast to the entire study area, which contains both tundra and boreal forest habitats, we forecast that the amount of AB in tundra alone will increase (0–61%) in the near term. Simulated high-quality caribou winter range, as indexed by tundra and spruce habitat that had not burned in ≥50 years, decreased modestly (<6%) in the near term over the entire study area. Simulated changes were more dramatic within the herd's core winter range, with declines in high-quality caribou winter range approaching 30%. Conversely, moose habitat was projected to increase by 19–64% within the core winter range in the near term. The simulated declines in the quantity of core winter range in the future due to larger and more frequent fires could impact caribou abundance through decreased nutritional performance and/or apparent competition with moose. These impacts would likely be detrimental to the subsistence users that rely on this resource. Additionally, changes in the fire regime and decreases in caribou abundance could amplify feedback mechanisms, such as decreasing albedo, by facilitating shrub growth that may hasten climate-driven changes to the composition and structure of vegetation communities in the low Arctic.
Concurrent Error Detection Using Watchdog Processors - A Survey

Concurrent system-level error detection techniques using a watchdog processor are surveyed. A watchdog processor is a small and simple coprocessor that detects errors by monitoring the behavior of a system. Like replication, it does not depend on any fault model for error detection. However, it requires less hardware than replication. It is shown that a large number of errors can be detected by monitoring the control flow and memory-access behavior. Two techniques for control-flow checking are discussed and compared with current error-detection techniques. A scheme for memory-access checking based on capability-based addressing is described. The design of a watchdog for performing reasonable checks on the output of a main processor by executing assertions is discussed. >