Miracle-dz/UniFAR-FTUs huggingface dataset:
Downregulation of platelet‐activating factor responsiveness during maturation of human dendritic cells

Dendritic cells (DCs) are specialized antigen‐presenting cells characterized by their ability to migrate into target sites, process antigens, and activate naive T‐cells. Biological activities of platelet‐activating factor (PAF) and the cytokine macrophage inflammatory protein‐3β (MIP‐3β) as well as the mRNA expression of their receptors were characterized in human DCs during lipopolysaccharide (LPS)‐promoted maturation. Platelet‐activating factor induced calcium transients, migration‐associated actin polymerization response, and chemotaxis in immature human dendritic cells differentiated in vitro from monocytes with interleukin‐4 and granulocyte macrophage colony stimulating factor. In addition, RT‐PCR experiments indicated mRNA expression of the PAF receptor in these immature DCs. Cell studies and mRNA analyses further revealed that immature DCs neither respond to MIP‐3β nor express its specific receptor, CCR7. Induction of cell differentiation by LPS led to the loss of the mRNA expression of the PAF receptor, accompanied by decreasing intracellular calcium release, actin polymerization, and migration after stimulation with PAF. In contrast, LPS treatment induced increasing responsiveness toward MIP‐3β and mRNA expression of CCR7. Comparable data regarding mRNA expression of PAF receptor and PAF responsiveness were also obtained with another maturation protocol using TNFα instead of LPS. The direct comparison between the two different protocols showed a slower decrease of PAF responsiveness induced by TNFα than by LPS. These results show the loss of PAF responsiveness associated with downregulation of PAF receptor mRNA expression during LPS‐ and TNFα‐induced maturation in human DCs. Therefore, these findings point to a functional relevance of PAF in recruiting immature DCs, whereas MIP‐3β might regulate the migration of DCs at a later stage of maturation. J. Cell. Physiol. 185:394–400, 2000. © 2000 Wiley‐Liss, Inc.
An Amino Acid Substitution in the Coding Region of the E2 Glycoprotein Adapts Ross River Virus To Utilize Heparan Sulfate as an Attachment Moiety

ABSTRACT Passage of Ross River virus strain NB5092 in avian cells has been previously shown to select for virus variants that have enhanced replication in these cells. Sequencing of these variants identified two independent sites that might be responsible for the phenotype. We now demonstrate, using a molecular cDNA clone of the wild-type T48 strain, that an amino acid substitution at residue 218 in the E2 glycoprotein can account for the phenotype. Substitutions that replaced the wild-type asparagine with basic residues had enhanced replication in avian cells while acidic or neutral residues had little or no observable effect. Ross River virus mutants that had increased replication in avian cells also grew better in BHK cells than the wild-type virus, whereas the remaining mutants were unaffected in growth. Replication in both BHK and avian cells of Ross River virus mutants N218K and N218R was inhibited by the presence of heparin or by the pretreatment of the cells with heparinase. Binding of the mutants, but not of the wild type, to a heparin-Sepharose column produced binding comparable to that of Sindbis virus, which has previously been shown to bind heparin. Replication of these mutants was also adversely affected when they were grown in a CHO cell line that was deficient in heparan sulfate production. These results demonstrate that amino acid 218 of the E2 glycoprotein can be modified to create an heparan sulfate binding site and this modification expands the host range of Ross River virus in cultured cells to cells of avian origin.
Citrus aurantium increases seizure latency to PTZ induced seizures in zebrafish thru NMDA and mGluR's I and II

Epilepsy is a serious neurological condition and pharmacotherapy is not effective for all patients and causes serious adverse effects and pharmacokinetic and pharmacodynamic interactions. Natural products and ethnobotanical resources can help develop new therapeutic options for conditions like epilepsy. In Puerto Rico, ethnobotanical resources highlight the anxiolytic properties of a tea like preparation made from the leaves of the Citrus aurantium tree or bitter orange. Studies performed with essential oils from the peel of the fruit have shown to increase seizure latency to pentylenetetrazole (PTZ) and maximal electroshock seizure in mice. We characterized the extract composition, and used a model of PTZ induces seizures in the zebrafish and a receptor-ligand binding assay to determine if this preparation has anticonvulsant properties and its mechanism of action. We determined that the aqueous extract made from the leaves of the C. aurantium tree contains hesperidin, neohesperidin, and neohesperidin dihydrochalcone. Using our zebrafish model, we determined that exposure to the C. aurantium 28 mg/mL extract in aquarium water increases seizure latency by 119% compared to controls. We ruled out a mechanism involving GABAA receptors using the selective antagonist gabazine. We used two approaches to study the role of glutamate in the mechanism of the C. aurantium extract. The ligand binding assay revealed C. aurantium extracts at concentrations of 0.42 to 5.6 mg/mL significantly reduced [3H]Glu binding indicating an interaction with glutamate receptors, in particular with NMDA receptors and mGluR II. This interaction was confirmed with our animal model using selective receptor antagonists and we identified an interaction with mGluR I, not observed in the ligand binding experiment. These study provide evidence of the anticonvulsant properties of the aqueous extract made from the leaves of the C. aurantium tree and a mechanism involving NMDA and mGluR's I and II.
A Broadband Doherty Power Amplifier Based on Continuous-Mode Technology

In this paper, a novel broadband Doherty power amplifier based on the continuous-mode technique (C-DPA) is proposed. The amplifier is focused on manipulating harmonic components in a Doherty power amplifier (DPA) structure to achieve improved bandwidth and efficiency. In a conventional DPA, harmonic isolation is typically required between the two transistors to prevent them from modulating each other at harmonic frequencies. However, as presented in this paper, such isolation is not actually necessary. On the contrary, by allowing the two transistors to modulate each other at harmonic frequencies with the help of a properly designed postharmonic tuning network, a series of highly efficient DPA modes can be created over a continuous frequency band, leading to a broadband C-DPA. Based on the proposed method, an example of a C-DPA working from 1.65 to 2.75 GHz was designed. According to the measured results, the designed C-DPA exhibits a 52%-66% efficiency at a -6 dB power backoff and a power utilization factor higher than 1.08 over the 1.1-GHz band. In addition, when simulated by a 7.5-dB peak-to-average power ratio 20-MHz LTE signal, the example C-DPA exhibits an efficiency of 46%-62% while maintaining an adjacent channel power ratio below -45 dBc after linearization over the full 1.1-GHz band. To the best of our knowledge, this is the first proposed C-DPA and a state-of-the-art performance for broadband DPAs.
Evidence for Genetic Exchange and Recombination of Rhizobium Symbiotic Plasmids in a Soil Population

A soil population of 16 Rhizobium leguminosarum bv. trifolii isolates was characterized by using three Sym (for symbiotic) plasmid-specific DNA hybridization probes: (i) an R. leguminosarum bv. trifolii-specific, repeated-sequence probe; (ii) a nifHDK gene probe, and (iii) a nod gene probe. A predominant Sym plasmid family was identified among the isolates. Three other unrelated Sym plasmid families were also identified. The isolates were also classified either by using a chromosomal DNA hybridization probe or by serological relatedness to 25 different R. leguminosarum bv. trifolii antisera. With either method, it was possible to group the 16 soil isolates into identical or related families. However, the correlation between the two techniques was not high. Irrespective of the means used to classify the bacterial host strain, it was possible to identify the same Sym plasmids in unrelated strains, as well as unrelated Sym plasmids in identical host strains. These data indicate that, within this soil population, there has been genetic exchange of Sym plasmids, and in one instance the hybridization pattern indicates that in vivo recombination of two different Sym plasmids may have occurred. Symbiotic effectiveness tests on red, strawberry, and subterranean clovers clearly differentiated the isolates. In general, the pattern of response was similar within groupings on the basis of Sym plasmid and chromosomal profiles but different between such groups.
Highly Educated Women Marry Less: An Analysis of Female Marriage Rates in Spain

The purpose of this article is to analyse how the increase of women's educational attainment has influenced marriage in Spain. It studies the transition from being single to marriage using data from the 1999 Fertility Survey and employing an event history analysis (EHA). The results indicate that highly educated women marry less. Moreover, the paper warns about the blind use of EHA in research that wishes to discriminate between the tempus and the quantum of the phenomenon under analysis.
Glucose Metabolism during Resting State Reveals Abnormal Brain Networks Organization in the Alzheimer’s Disease and Mild Cognitive Impairment

This paper aims to study the abnormal patterns of brain glucose metabolism co-variations in Alzheimer disease (AD) and Mild Cognitive Impairment (MCI) patients compared to Normal healthy controls (NC) using the Alzheimer Disease Neuroimaging Initiative (ADNI) database. The local cerebral metabolic rate for glucose (CMRgl) in a set of 90 structures belonging to the AAL atlas was obtained from Fluro-Deoxyglucose Positron Emission Tomography data in resting state. It is assumed that brain regions whose CMRgl values are significantly correlated are functionally associated; therefore, when metabolism is altered in a single region, the alteration will affect the metabolism of other brain areas with which it interrelates. The glucose metabolism network (represented by the matrix of the CMRgl co-variations among all pairs of structures) was studied using the graph theory framework. The highest concurrent fluctuations in CMRgl were basically identified between homologous cortical regions in all groups. Significant differences in CMRgl co-variations in AD and MCI groups as compared to NC were found. The AD and MCI patients showed aberrant patterns in comparison to NC subjects, as detected by global and local network properties (global and local efficiency, clustering index, and others). MCI network’s attributes showed an intermediate position between NC and AD, corroborating it as a transitional stage from normal aging to Alzheimer disease. Our study is an attempt at exploring the complex association between glucose metabolism, CMRgl covariations and the attributes of the brain network organization in AD and MCI.
Exploring the microbial diversity in Jordanian hot springs by comparative metagenomic analysis

A culture‐independent approach was utilized in this study to reveal the microbial diversity in Jordanian hot springs represented by Ma'in and Afra hot springs. Water samples from Ma'in and Afra hot springs were collected in June 2015. The in situ temperature of water samples range was 38–59°C and the pH range was 7.4–8.4. The metagenome was extracted and analyzed using the next generation technology (bTEFAP®). A total of 314,310 sequences were parsed and 288,452 were then clustered. The sequences were predominated by bacteria (>84%) and the relative abundance of archaea in each sample was <1%. Eukaryotic microorganisms were detected but with varying abundances (0.6%–15%). Because most of the detected sequences were found to belong to the domain of bacteria (196,936 sequences out 288,452), the bacterial sequences were utilized for further microbial analyses. With respect to alpha and beta diversity, samples were rarefied to 30,000 sequences and bootstrapped at 10,000 sequences. The Shannon–Wiener Index curve plot reaches a plateau at approximately 3,000 sequences indicating that sequencing depth was sufficient to capture the full scope of microbial diversity. By examining the relative abundance of phyla detected in each sample, it appears that the biota of both Jordanian hot springs sampled are compositionally similar, with over 50% of the microbial community of each sample being comprised of the phylum Proteobacteria. The second most abundant phylum was the phylum Bacteroidetes which represents more than 13% in each sample. The phylum Firmicutes was also detected with a significant abundance. However, lower abundance of Deinococcus, Verrucomicrobia, Planctomycetes, and Chloroflexi was detected. A principal coordinate analysis plot was generated based upon the weighted UniFrac distance matrix. By utilizing Monte Carlo simulations, we were able to determine that there were no significant differences in the microbial diversity between each sample.
Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome

Objective Irritable bowel syndrome (IBS) is a common clinical gastrointestinal dysfunction disorders. 5-sertonon (5-hydroxytryptamine, 5-HT) is a very important neurotransmitter, which is involved in gastrointestinal motion and sensation. Solute carrier family 6 member 4 (SLC6A4) gene encode serotonin transporter (SERT) which function is to rapidly reuptake the most of 5-HT. Therefore, it is needed to explore the association between SLC6A4 gene polymorphisms and IBS. Methods 119 patients and 238 healthy controls were administrated to detect the SLC6A4 gene polymorphisms including 5-HT-transporter-gene-linked polymorphic region (5-HTTLPR), variable number of tandem repeats (VNTRs) and three selected tag Single Nucleotide Polymorphisms (SNPs) rs1042173, rs3794808, rs2020936 by using polymerase chain reaction (PCR) and TaqMan® SNP Genotyping. Results There were significant difference for 5-HTTLPR between IBS and control groups (X2 = 106.168, P<0.0001). In control group, genotypes were mainly L/L (58.4%), however, the genotypes in IBS were S/S (37.8%). The significant difference was shown in D-IBS subjects when compared to the controls (X2 = 50.850, P<0.0001) for 5-HTTLPR. For STin2 VNTR, rs1042173, rs3794808, and rs2020936 polymorphisms, there were no any significant differences between IBS and control groups. There were no statistical significantly haplotypes for 5-HTTLPR, VNTRs and the three SNPs between IBS and controls. Conclusion The S allele in 5-HTTLPR was a susceptible allele with Chinese Han IBS, but other associations of VNTRs, three selected Tag SNPs and positive haplotype with IBS were not found. It is indicated that much research are needed to study the relationship between other polymorphisms in SLC6A4 gene and IBS.
In Defense of Shame: Shame in the Context of Guilt and Embarrassment

We are interested in the relations among shame, guilt, and embarrassment and especially in how each relates to judgments of character. We start by analyzing the distinction between being and feeling guilty, and unearth the role of shame as a guilt feeling. We proceed to examine shame and guilt in relation to moral responsibility and to flaws of character. We address a recent psychological finding (Tangney, Wagner, Hill-Barlow, and Marshall, 1996; Tangney, Hill-Barlow, Wagner and Marshall, 1996) that shame is both destructive and in so far as it has a social function could be replaced by guilt. We reinterpret the guilt culture/shame culture distinction in terms of our way of distinguishing these emotions. Finally we examine embarrassment as distinct from shame and find the difference to lie not so much in the phenomenology of the participant as it is in context, and in which elements of the context the speaker describing the emotion wishes to stress. We conclude by defending shame despite its psychological troubles.
The relationships between time use and health and well‐being in men with spinal cord injury

Abstract Despite evidence of the relationship between time use and health and well‐being, and the World Health Organization's recognition of activity limitation as a measure of disability, there has been limited investigation into the activity patterns of persons with disabilities. This study used interviews and time diaries to provide preliminary descriptive and analytical information about the daily time use patterns of men with a spinal cord injury (SCI) living in the community (n=312). The study examined relationships between disability severity, time use, social support and socio‐economic status, and psychosocial outcomes, specifically life satisfaction, perceived health and adjustment to disability. The men with spinal cord injury spent on average 7.2 hours in leisure activities; 4.7 hours in productivity; 3.7 hours in personal care; and 8.5 hours sleeping. The sample rated their satisfaction with their time use as mediocre, but levels of adjustment to disability were moderate to high. Regression an.
Matrix quality and disturbance frequency drive evolution of species behavior at habitat boundaries

Summary Previous theoretical studies suggest that a species' landscape should influence the evolution of its dispersal characteristics, because landscape structure affects the costs and benefits of dispersal. However, these studies have not considered the evolution of boundary crossing, that is, the tendency of animals to cross from habitat to nonhabitat (“matrix”). It is important to understand this dispersal behavior, because of its effects on the probability of population persistence. Boundary‐crossing behavior drives the rate of interaction with matrix, and thus, it influences the rate of movement among populations and the risk of dispersal mortality. We used an individual‐based, spatially explicit model to simulate the evolution of boundary crossing in response to landscape structure. Our simulations predict higher evolved probabilities of boundary crossing in landscapes with more habitat, less fragmented habitat, higher‐quality matrix, and more frequent disturbances (i.e., fewer generations between local population extinction events). Unexpectedly, our simulations also suggest that matrix quality and disturbance frequency have much stronger effects on the evolution of boundary crossing than either habitat amount or habitat fragmentation. Our results suggest that boundary‐crossing responses are most affected by the costs of dispersal through matrix and the benefits of escaping local extinction events. Evolution of optimal behavior at habitat boundaries in response to the landscape may have implications for species in human‐altered landscapes, because this behavior may become suboptimal if the landscape changes faster than the species' evolutionary response to that change. Understanding how matrix quality and habitat disturbance drive evolution of behavior at boundaries, and how this in turn influences the extinction risk of species in human‐altered landscapes should help us identify species of conservation concern and target them for management.
Ground-based Icing Condition Remote Sensing System Definition Ground-based Icing Condition Remote Sensing System Definition Ground-based Icing Condition Remote Sensing System Definition Background

This report documents the NASA Glenn Research Center activities to assess and down select remote sensing technologies for the purpose of developing a system capable of measuring icing condition hazards aloft. The information generated by such a remote sensing system is intended for use by the entire aviation community, including flight crews, air traffic controllers, airline dispatchers, and aviation weather forecasters. The remote sensing system must be capable of remotely measuring temperature and liquid water content (LWC) and indicating the presence of super-cooled large droplets (SLD). Technologies examined include Profiling Microwave Radiometer, Dual-Band Radar, Multi-Band Radar, Ka-Band Radar, Polarized Ka-Band Radar, and Multiple Field of View (MFOV) Lidar. The assessment of these systems took place primarily during the Mt. Washington Icing Sensors Project (MWISP) in April 1999 and the Alliance Icing Research Study (AIRS) from November 1999 through February 2000. A discussion of the various sensing technologies is included. The result of the assessment is that no one sensing technology can satisfy all of the stated project goals. Therefore a proposed system includes radiometry and Ka-band radar. A multilevel approach is proposed to allow the future selection of the fielded system based upon required capability and available funding. The most basic level system would be the least capable and least expensive. The next level would increase capability and cost, and the highest level would be the most capable and most expensive to field. The Level 1 system would consist of a Profiling Microwave Radiometer. The Level 2 system would add a Ka-Band Radar. The Level 3 system would add polarization to the Ka-Band Radar. All levels of the system would utilize hardware that is already under development by the U.S. Government. However, to meet the needs of the aviation community, all levels of the system will require further development. In addition to the proposed system, it is also recommended that NASA continue to foster the development of Multi-Band Radar and airborne microwave radiometer technologies.
Validation of Scores on the Homework Management Scale for Middle School Students

The purpose of this study was to test the validity of scores on the Homework Management Scale (HMS) using 699 rural and 482 urban eighth graders. The study revealed that the HMS comprised 5 separate yet related factors: arranging the environment, managing time, handling distraction, monitoring motivation, and controlling emotion. Given an adequate level of configural, factor loading, common error covariance, and intercept invariance, I further tested the difference between group means. Results revealed that urban students were more likely to manage their homework than their rural counterparts in 2 of the 5 areas, namely, handling distraction and monitoring motivation. Findings also showed that the HMS differentiated among students who were more or less likely to complete homework assignments.
The Family-Centered Program Rating Scale

Programs that provide services for young children with special needs are now planning ways to support the child's family as well. In response to the great diversity in the way programs define and implement a family-centered approach to service delivery, researchers at the Beach Center on Families and Disability designed and tested the Family-Centered Program Rating Scale (FamPRS). In addition to being used as a component of a comprehensive program evaluation, the FamPRS may be used for program planning, self development, and research. This article describes how the rating scale was developed, provides information about its psychometric properties, and makes recommendations for its appropriate uses.