Miracle-dz/UniFAR-FTUs huggingface dataset:
Intermediate filaments and the initiation of desmosome assembly

The desmosome junction is an important component in the cohesion of epithelial cells, especially epidermal keratinocytes. To gain insight into the structure and function of desmosomes, their morphogenesis has been studied in a primary mouse epidermal (PME) cell culture system. When these cells are grown in approximately 0.1 mM Ca2+, they contain no desmosomes. They are induced to form desmosomes when the Ca2+ level in the culture medium is raised to approximately 1.2 mM Ca2+. PME cells in medium containing low levels of Ca2+, and then processed for indirect immunofluorescence using antibodies directed against desmoplakins (desmosomal plaque proteins), display a pattern of discrete fluorescent spots concentrated mainly in the perinuclear region. Double label immunofluorescence using keratin and desmoplakin antibodies reveals that the desmoplakin-containing spots and the cytoplasmic network of tonofibrils (bundles of intermediate filaments [IFB]) are in the same juxtanuclear region. Within 1 h after the switch to higher levels of Ca2+, the spots move toward the cell surface, primarily to areas of cell-cell contact and not to free cell surfaces. This reorganization occurs at the same time that tonofibrils also move toward cell surfaces in contact with neighboring cells. Once the desmoplakin spots have reached the cell surface, they appear to aggregate to form desmosomes. These immunofluorescence observations have been confirmed by immunogold ultrastructural localization. Preliminary biochemical and immunological studies indicate that desmoplakin appears in whole cell protein extracts and in Triton high salt insoluble residues (i.e., cytoskeletal preparations consisting primarily of IFB) prepared from PME cells maintained in medium containing both low and normal Ca2+ levels. These findings show that certain desmosome components are preformed in the cytoplasm of PME cells. These components undergo a dramatic reorganization, which parallels the changes in IFB redistribution, upon induction of desmosome formation. The reorganization depends upon both the extracellular Ca2+ level and the establishment of cell-to-cell contacts. Furthermore, the data suggests that desmosomes do not act as organizing centers for the elaboration of IFB. Indeed, we postulate that the movement of IFB and preformed desmosomal components to the cell surface is an important initiating event in desmosome morphogenesis.
The Influences of Parental Expectations on Children's Academic Achievements: A Comparative Analysis of the United States and China

Parenting is very important to children, and it directly or indirectly influences children's outcomes in schools. Parents express passions and strong desires to help their children to succeed regardless of race, ethnicity, or socioeconomic status, and they are highly interested in their children's education in school. This paper is concerned with parental expectations for school-aged children in the United States and China under the influences of historical, cultural and social context. I will comprehensively compare relevant literature parts in both America and China, determining the distinctions in terms of various parenting patterns. Research about parental expectations in the two countries will help me to better understand the differences of parental involvement in education and understand some problems and phenomena in current society. The methodology is based on theoretical/conceptual literature, and it is not an empirical study.
Diurnal variations in luminal and stromal areas of choroid in normal eyes

Aims To determine the diurnal variations of the luminal and stromal areas of the choroid in normal eyes. Methods This was a prospective observational study of 38 eyes of 38 normal subjects. The blood pressure, heart rate, intraocular pressure and enhanced depth imaging optical coherence tomographic (EDI-OCT) images were recorded every 3 hours between 6:00 and 21:00 hours. The horizontal EDI-OCT images of the subfoveal choroid were converted to binary images. The central choroidal thickness (CCT), total cross-sectional choroidal area, the luminal areas, stromal areas and the ratio of luminal area to total choroidal area (L/C ratio) were determined. Results There were significant diurnal variations in the CCT, total choroidal area, luminal area and L/C ratio with the maximum values at 6:00 hours and the minimum values at 15:00 hours (p<0.001 for the CCT, p=0.011 for the total choroidal area, p<0.001 for the luminal area and p=0.014 for the L/C ratio). There was no significant variation in the stromal area (p=0.216). The range of fluctuation in the CCT was significantly correlated with that in the luminal area and the total choroidal area (p<0.001). However, there was no significant correlation between the fluctuation range in the CCT and that in the stromal area (p=0.095). There was no statistical relationship between the systemic parameters and the choroidal parameters. Conclusions The changes in the luminal area are most likely responsible for the diurnal change in the CCT and subfoveal choroidal area. Trial registration number UMIN000019060, Pre-results.
Academic, Social and Psychological Correlates of Gender Disparity in Higher Education: The Case of Debub University

The present study investigates the pattern of female academic success in higher education and their prospects in progressing through the system drawing a solid data on their academic status, socio-cultural and psychological factors, and personal background variables. The sample involved 134 (Male= 94 and Female = 40) first year students admitted in the 2003/2004 academic year. The methods used to collect information include students’ academic performance records, and a self-administered measure assessing respondents’ gender role attitude, general academic self concept, and perceived parental expectation. The results indicate that with the exception of College of Health Sciences, the attrition rate ranges from33% in the Faculty of Natural Sciences to as high as 46.7% in the College of Agriculture among girls who joined Debub University after the pre-university preparatory program. Further, the analyses generally indicate that females have low academic self-concept and are particularly less confident about their ability in traditionally masculine subjects like Mathematics and Physics. The low self-concept of ability in case of girls was related to their low academic performance. But boys and girls did not significantly vary in their gender role attitude indicating pervading traditional stereotypic beliefs. Finally, implications of the findings relevant to address gender equity in higher education institutions in Ethiopia are discussed. ----------------------------------------------------------------------------------------------------------------
Unilateral changes in walking surface compliance evoke dorsiflexion in paretic leg of impaired walkers

Introduction Gait impairments due to stroke impact millions of individuals throughout the world. Despite the growing interest in automating gait therapy with robotic devices, there is no clear evidence that robot-assisted gait therapy is superior to traditional treadmill-based therapy. Methods This work investigates the effect of perturbations to the compliance of the walking surface on the paretic leg of impaired walkers. Using a novel robotic device, the variable stiffness treadmill, we apply perturbations to the compliance of the walking surface underneath the non-paretic leg of two hemi-paretic walkers and analyze the kinematic and neuromuscular response of the contralateral (paretic) leg with motion capture and surface electromyography systems. Results We present results of evoked muscle activity (predominately tibialis anterior) and increased dorsiflexion in the paretic leg during the swing phase of gait at stiffness values of 60 kN/m and less for all subjects. Conclusions This work provides evidence for the first time of reducing the drop-foot effect in the impaired leg of hemiparetic walkers in response to unilateral perturbations to the compliance of the treadmill platform, thus providing direction for targeted robot-assisted gait rehabilitation.
Return Maps, Parameterization, and Cycle-Wise Planning of Yo-Yo Playing

This note presents a unified approach to nominal control generation and return map parameterization for yo-yo playing. The approach is based on choosing an intermediate state of the yo-yo as the parameter and formulating an optimization problem with appropriate boundary conditions that incorporate the corresponding parameter. By solving the optimization problems, both the return map and the nominal optimal control for the robot can be obtained. The return map is naturally parameterized with the intermediate state, which can be treated as a virtual control. The return map facilitates the application of standard analysis and design methods for discrete-time control systems. The approach is promising as it might be extended to control other cyclic dynamic systems.
Wages and Job Mobility of Young Workers

This paper presents a discrete-time version of Jovanovic's model of worker-firm matching. Descriptive evidence is presented that supports the notion that unobserved worker-firm heterogeneity is an important component in the intertemporal structure of wages for young workers. A structural econometric model of wage dynamics under worker-firm sorting is developed and estimated. Finally, a formal test of the matching model is carried out, and the matching structure on intertemporal covariances of wages is not rejected. My results indicate the necessity of jointly considering processes of turnover and wage growth when analyzing the labor market experiences of young workers.
High Genetic Diversity and Adaptive Potential of Two Simian Hemorrhagic Fever Viruses in a Wild Primate Population

Key biological properties such as high genetic diversity and high evolutionary rate enhance the potential of certain RNA viruses to adapt and emerge. Identifying viruses with these properties in their natural hosts could dramatically improve disease forecasting and surveillance. Recently, we discovered two novel members of the viral family Arteriviridae: simian hemorrhagic fever virus (SHFV)-krc1 and SHFV-krc2, infecting a single wild red colobus (Procolobus rufomitratus tephrosceles) in Kibale National Park, Uganda. Nearly nothing is known about the biological properties of SHFVs in nature, although the SHFV type strain, SHFV-LVR, has caused devastating outbreaks of viral hemorrhagic fever in captive macaques. Here we detected SHFV-krc1 and SHFV-krc2 in 40% and 47% of 60 wild red colobus tested, respectively. We found viral loads in excess of 106–107 RNA copies per milliliter of blood plasma for each of these viruses. SHFV-krc1 and SHFV-krc2 also showed high genetic diversity at both the inter- and intra-host levels. Analyses of synonymous and non-synonymous nucleotide diversity across viral genomes revealed patterns suggestive of positive selection in SHFV open reading frames (ORF) 5 (SHFV-krc2 only) and 7 (SHFV-krc1 and SHFV-krc2). Thus, these viruses share several important properties with some of the most rapidly evolving, emergent RNA viruses.
Purinergic and muscarinic modulation of ATP release from the urothelium and its paracrine actions

The urothelium is a newly recognized sensory structure that detects bladder fullness. Pivotal to this sensory role is the release of ATP from the urothelium. However, the routes for urothelial ATP release, its modulation by receptor-mediated pathways, and the autocrine/paracrine role of ATP are poorly understood, especially in native tissue. We examined the action of key neurotransmitters: purinergic and muscarinic agonists on ATP release and its paracrine effect. Guinea pig and human urothelial mucosa were mounted in a perfusion trough; superfusate ATP was measured using a luciferin-luciferase assay, and tissue contractions were recorded with a tension transducer. Intracellular Ca2+ was measured in isolated urothelial cells with fura-2. The P2Y agonist UTP but not the P2X agonist α,β-methylene-ATP generated ATP release. The muscarinic agonist carbachol and the M2-preferential agonist oxotremorine also generated ATP release, which was antagonized by the M2-specific agent methoctramine. Agonist-evoked ATP release was accompanied by mucosal contractions. Urothelial ATP release was differentially mediated by intracellular Ca2+ release, cAMP, exocytosis, or connexins. Urothelium-attached smooth muscle exhibited spontaneous contractions that were augmented by subthreshold concentrations of carbachol, which had little direct effect on smooth muscle. This activity was attenuated by desensitizing P2X receptors on smooth muscle. Urothelial ATP release was increased in aging bladders. Purinergic and muscarinic agents produced similar effects in human urothelial tissue. This is the first demonstration of specific modulation of urothelial ATP release in native tissue by purinergic and muscarinic neurotransmitters via distinct mechanisms. Released ATP produces paracrine effects on underlying tissues. This process is altered during aging and has relevance to human bladder pathologies.
The Polycomb Group Protein Ring1b/Rnf2 Is Specifically Required for Craniofacial Development

Polycomb group (PcG) genes are chromatin modifiers that mediate epigenetic silencing of target genes. PcG-mediated epigenetic silencing is implicated in embryonic development, stem cell plasticity, cell fate maintenance, cellular differentiation and cancer. However, analysis of the roles of PcG proteins in maintaining differentiation programs during vertebrate embryogenesis has been hampered due to the early embryonic lethality of several PcG knock-outs in the mouse. Here, we show that zebrafish Ring1b/Rnf2, the single E3 ubiquitin ligase in the Polycomb Repressive Complex 1, critically regulates the developmental program of craniofacial cell lineages. Zebrafish ring1b mutants display a severe craniofacial phenotype, which includes an almost complete absence of all cranial cartilage, bone and musculature. We show that Cranial Neural Crest (CNC)-derived cartilage precursors migrate correctly into the pharyngeal arches, but fail to differentiate into chondrocytes. This phenotype is specific for cartilage precursors, since other neural crest-derived cell lineages, including glia, neurons and chromatophores, are formed normally in ring1b mutants. Our results therefore reveal a critical and specific role for Ring1b in promoting the differentiation of cranial neural crest cells into chondrocytes. The molecular mechanisms underlying the pathogenesis of craniofacial abnormalities, which are among the most common genetic birth defects in humans, remain poorly understood. The zebrafish ring1b mutant provides a molecular model for investigating these mechanisms and may lead to the discovery of new treatments or preventions of craniofacial abnormalities.
Globalisation and ethnic jokes: A new look on an old tradition in Belarus and Estonia

Christie Davies, the renowned humour researcher and a passionate propagator of the comparative method in studying jokes, stressed the necessity of establishing a relationship between two sets of social facts: the jokes themselves on the one hand, and the social structure or cultural traditions wherein they disseminate on the other (Davies 2002: 6). He also inspired others to examine the differences and similarities in the patterns of jokes between different nations, social circumstances and eras. By doing this and building falsifiable models and generalisations of joking relationships, he changed the way we look at and analyse ethnic jokes. This study returns to earlier findings of Estonian (Laineste 2005, 2009) and Belarusian (Astapova 2015, Zhvaleuskaya 2013, 2015) ethnic jokes and takes a look at new trends in fresh data. Starting with the jokes from the end of the 19th century and ending with the most recent jokes, memes and other humorous items shared over the Internet, the paper will give an overview of how social reality interacts with the rules of target choice, above all describing the effect of globalisation on jokelore.
The Moderating Effect of Gender in Urgent Care Service Satisfaction

The objective of this study is to investigate the extent to which gender serves as a moderator in the relationships between healthcare service quality, patient satisfaction, and behavioral intention in the urgent care industry. Using the data collected from 253 male and 209 female urgent care patients, the moderating effect of gender was examined through partial least square structural equation modeling. Gender was found significant in the relationship between urgent care quality and patient satisfaction. To improve patient satisfaction among males, urgent care providers should focus on reducing negative emotions among male patients. This can be done by reducing the gap between their expectations and their perceptions, creating a warm environment in the urgent care facility, and alleviating patients' emotional vulnerability. The female community is more willing to pay a premium price and recommend the urgent care providers if they are satisfied. The practical implication is that urgent care providers should employ different strategies across gender to improve their perceptions of care quality and satisfaction level.
Recognizing Programming Misconceptions - An analysis of the data collected from the UUhistle program simulation tool

Lic.sc. (Tech) Juha Sorva Learning to program has many challenges. If a student encounters problems of understanding how the programming language works or how a program is executed, it might be hard to fix these misconceptions later. These misconceptions may also make it hard to learn more complicated concepts if the student does not understand the basics correctly. In program simulation exercises a student takes on the role of the computer as executor of a program using a graphical user interface. In these exercises, students should be able to understand the execution model to simulate the execution correctly and therefore simulation mistakes are interesting because they can reveal what kind of misconceptions students may have. The main research question of this master's thesis is to analyze log files collected by UUhistle program simulation tool used in the basic programming course and try to find out what the most common simulation errors the students have made are and try to figure out the causes of the errors. Is the error a simple mistake, is it caused by the user interface, is the error related to the simulation exercises or can we find a similar programming misconception in the literature? We selected common 26 errors which were likely caused by something else than a simple mistake. The errors are related to the basics of the execution, conditions, loops, functions and object-oriented programming. Many of these errors are similar to those misconceptions reported in the literature earlier. Therefore we can make an assumption that visual program simulation makes it possible to recognize possible misconceptions and be used to fix the misconceptions by giving feedback to students. We also present a few ideas how to improve UUhistle based on the results we got. Some of the errors are clearly related to the user interface and by making some changes to the interface we can reduce the number of the errors caused by the tool itself. We also noticed that the students did not read much the feedback UUhistle gave to them and we should also improve the way UUhistle shows the feedback.
Systematic Review and Meta-Analysis of Human Studies to Support a Quantitative Recommendation for Whole Grain Intake in Relation to Type 2 Diabetes

Background Due to the increasing evidence of their health benefits, whole grains are recommended for consumption worldwide. Such recommendations are, however, rarely quantitative. Our aim was to perform a quantitative evaluation of the relationship between whole grain consumption and the occurrence of type 2 diabetes (T2D) to support a recommendation on the daily consumption of whole grains. Methods and Findings We conducted a systematic review by searching three bibliographic databases. We included human studies addressing the relationship between whole grain consumption and T2D occurrence, and providing quantitative information on daily intake of whole grains. A dose-response meta-regression analysis between whole grain intake and T2D occurrence was performed, using a hierarchical mixed least square linear regression model. Eight observational studies were included (all but one prospective), with a total of 15,573 cases of T2D among 316,051 participants. Quantitative meta-regression demonstrated a significant linear inverse relationship between whole grain intake and T2D occurrence (P<0.0001), with an overall absolute reduction of 0.3% in the T2D rate for each additional 10 g of whole grain ingredient consumed daily. The association persisted when adjusted on sex, age, country, study design, follow up duration, and mode of report of whole grain intakes (as foods or ingredients). Conclusions The meta-regression model made it possible to estimate the decrease in T2D risk corresponding to various changes in whole grain intakes, and the results contribute to setting up quantitative recommendations. For instance, consuming three servings of whole grain foods (45 g of whole grain ingredients) daily would induce a 20% relative reduction in the T2D risk as compared to consuming a half serving (7.5 g of whole grain ingredients). These results should be considered for future recommendations, by considering the actual whole grain intake of the concerned populations. The systematic review protocol was published on the PROSPERO register (CRD42013006925).
Contrasting demographic history and phylogeographical patterns in two Indo‐Pacific gastropods

Marine species with ranges that span the Indo‐Australian Archipelago (IAA) exhibit a range of phylogeographical patterns, most of which are interpreted in the context of vicariance between Indian and Pacific Ocean populations during Pliocene and Pleistocene low sea‐level stands. However, patterns often vary among ecologically similar taxa, sometimes even within genera. This study compares phylogeographical patterns in two species of highly dispersive neritid gastropod, Nerita albicilla and Nerita plicata, with nearly sympatric ranges that span the Indo‐Pacific. Mitochondrial COI sequences from > 1000 individuals from 97 sites reveal similar phylogenies in both species (two divergent clades differing by 3.2% and 2.3%, for N. albicilla and N. plicata, respectively). However, despite ecological similarity and congeneric status, the two species exhibit phylogeographical discordance. N. albicilla has maintained reciprocal monophyly of Indian and Pacific Ocean populations, while N. plicata is panmictic between oceans, but displays a genetic cline in the Central Pacific. Although this difference might be explained by qualitatively different demographic histories, parameter estimates from three coalescent models indicate that both species have high levels of gene flow between demes (2Nem > 75), and share a common history of population expansion that is likely associated with cyclical flooding of continental shelves and island lagoons following low sea‐level stands. Results indicate that ecologically similar, codistributed species may respond very differently to shared environmental processes, suggesting that relatively minor differences in traits such as pelagic larval duration or microhabitat association may profoundly impact phylogeographical structure.