Miracle-dz/UniFAR-FTUs huggingface dataset:
Yolk-sac–derived macrophages regulate fetal testis vascularization and morphogenesis

Significance A main requisite for organogenesis is the integration of vascular networks, which not only deliver oxygen and nutrients but also serve instructive roles in organ patterning that determine how organs develop into their final structure and function in the adult. Our previous work showed that vascularization is essential to induce formation of the primitive cord structures that give rise to the seminiferous tubules of the adult testis. Here we show that fetal macrophages are required to remodel the vasculature and refine organ compartments during differentiation of the gonad. This study reveals an underappreciated and likely vital role for macrophages in fetal organogenesis that may be relevant to the development of many organs. Organogenesis of the testis is initiated when expression of Sry in pre-Sertoli cells directs the gonad toward a male-specific fate. The cells in the early bipotential gonad undergo de novo organization to form testis cords that enclose germ cells inside tubules lined by epithelial Sertoli cells. Although Sertoli cells are a driving force in the de novo formation of testis cords, recent studies in mouse showed that reorganization of the vasculature and of interstitial cells also play critical roles in testis cord morphogenesis. However, the mechanism driving reorganization of the vasculature during fetal organogenesis remained unclear. Here we demonstrate that fetal macrophages are associated with nascent gonadal and mesonephric vasculature during the initial phases of testis morphogenesis. Macrophages mediate vascular reorganization and prune errant germ cells and somatic cells after testis architecture is established. We show that gonadal macrophages are derived from primitive yolk-sac hematopoietic progenitors and exhibit hallmarks of M2 activation status, suggestive of angiogenic and tissue remodeling functions. Depletion of macrophages resulted in impaired vascular reorganization and abnormal cord formation. These findings reveal a previously unappreciated role for macrophages in testis morphogenesis and suggest that macrophages are an intermediary between neovascularization and organ architecture during fetal organogenesis.
New design for reducing interfacial stresses of reinforced structures with FRP plates

Purpose The sustainability of the structures is not only a technical goal, but also a matter of social and environmental values. This requires the researchers to use very rigid, highly durable and corrosion-resistant composite structures in order to achieve the technical, environmental and social goals. The purpose of this paper is to present an original work on reducing the interfacial stresses of bonded structures with fibre-reinforced polymers (FRP) plates based on new taper design. Design/methodology/approach In this proposed concept, the effect of combined taper is investigated on reducing interfacial stresses, attempting to enhance the structure performance and address the debonding problem that comes with reinforcing techniques. This research is carried out by using finite element analysis, incorporating many new parameters. Findings As a result, a new solution is discovered that combined taper in both adhesive layer and composite laminate, which significantly reduces the interfacial stresses at the end of the FRP plate. Additionally, a parametric study is carried out in order to determine the optimal configurations of taper dimensions as well as other parameters that influence the stress concentration distribution at the edge of the adherends. Practical implications This new design regarding the reduction of interfacial stresses will help in increasing the lifespan of damaged structures reinforced by FRP composites, preserving thus its technical, historical and social values. Originality/value The paper uses straight, concave and convex fillets with inverse taper as a new design solution with new parameters including thermo-mechanical loads and pre-stressed FRP plate with multi-layer, fibre orientation and shear-lag effects.
Protocol for Management of Imported Pediatric Malaria Decreases Time to Medication Administration

Background: A malaria management protocol was developed and implemented at a tertiary care children's hospital in September 1999. We retrospectively evaluated children admitted with malaria 10-years preimplementation and 7-years postimplementation to determine the impact the protocol had on management and time delay to appropriate antimalarial therapy. Methods: This before and after study compared all admissions with the discharge diagnosis of malaria in the study period. Retrospective chart review was used to determine the time from emergency department (ED) registration to administration of antimalarial treatment. Other outcomes measured included mortality, length of hospital stay, and intensive care unit admission. Results: Fifty-eight admissions were identified during the defined period, most of which were due to Plasmodium falciparum[r] malaria. Thirty-one (53.4%) cases were before implementation of the protocol. Children were more likely to receive appropriate investigations to assess for possible severe malaria before transfer from the ED to the ward after protocol implementation (18% vs. 63%, P = 0.005). Analysis of index cases of malaria, excluding patients diagnosed after the diagnosis of a sibling, showed there was a significant reduction in time to medication administration (8 vs. 5.5 hours, P = 0.036). Conclusion: After broad-based implementation of a malaria treatment protocol in a pediatric hospital, children received more thorough investigations, were more likely to receive therapy before leaving the ED and had a shorter delay before receiving appropriate antimalarial therapy.
Four Non-Gaussian-Type Tube Models of Rubber-Like Elasticity: Analysis of Tensile Data for Lightly and Highly Carbon-Black Filled Styrene-Butadiene Elastomers

Abstract Recently, using a new, non-Gaussian, tube-based entropy model for active-filler filled elastic networks with the polymer phase influenced by the filler loading, proposed by the author, a data analysis of literature tensile data for carbon-black filled styrene-butadiene rubber (SBR) networks of different loadings was carried out; the data-fittability and the particular influence of the filler loading were examined in detail. In this report, the data-fitting performances of three other non-filled network models, all tube-based and finite chain extensible, along with a common strain-amplification factor that is necessary to extend them to the analysis of the filled networks, as well as the model which was employed in the above study were compared. As tensile data for this analysis, those observed for the same unfilled SBR, the same lightly loaded SBR and one of the same highly loaded SBR networks, as in our previous study, were chosen.
Ultrasound Versus Anatomic Guidance for Intra-articular and Periarticular Injection: A Systematic Review

Abstract Objective: To investigate the clinical efficacy of ultrasound when compared with anatomic standard injection using palpation/anatomic landmarks. Methods: PubMed, Ovid Medline/Cochrane Reviews, BIOSIS Previews®, and SPORTDiscus™ databases were searched to January 2011. To narrow the search, the following key search terms were used: ultrasound, guided, injection, joint, shoulder, elbow, wrist, hip, knee, and ankle. Fifteen articles were determined to be relevant, and an additional 2 articles were added after bibliography sections of the 15 articles were reviewed, resulting in a total of 17 articles meeting inclusion and exclusion criteria. Results: Accuracy was greater in the ultrasound-guided group, independent of anatomic site. An improvement in time to symptom onset in the short term (< 6 weeks) was greatest with the use of ultrasound-guided intra-articular injection. Long-term outcomes are not proven to be different between intra-articular injection with ultrasound or anatomic guidance. The greatest trends were observed in the knee and shoulder joints, demonstrating improvements in function, pain, intensity, and range of motion, independent of injection technique. In foot/ankle and wrist/hand, small joint space injections showed a greater accuracy with ultrasound-guided injections contrasted to larger joint spaces, in which these joint injections demonstrated equal accuracy independent of injection technique. Conclusion: This systematic review can confirm that accuracy is improved with the use of ultrasound-guided intra-articular injection. We can also confirm that short-term outcome improvements are present using ultrasound-guided injection techniques but can confirm no difference in long-term outcome measures using either technique.
Modelling Trial-by-Trial Changes in the Mismatch Negativity

The mismatch negativity (MMN) is a differential brain response to violations of learned regularities. It has been used to demonstrate that the brain learns the statistical structure of its environment and predicts future sensory inputs. However, the algorithmic nature of these computations and the underlying neurobiological implementation remain controversial. This article introduces a mathematical framework with which competing ideas about the computational quantities indexed by MMN responses can be formalized and tested against single-trial EEG data. This framework was applied to five major theories of the MMN, comparing their ability to explain trial-by-trial changes in MMN amplitude. Three of these theories (predictive coding, model adjustment, and novelty detection) were formalized by linking the MMN to different manifestations of the same computational mechanism: approximate Bayesian inference according to the free-energy principle. We thereby propose a unifying view on three distinct theories of the MMN. The relative plausibility of each theory was assessed against empirical single-trial MMN amplitudes acquired from eight healthy volunteers in a roving oddball experiment. Models based on the free-energy principle provided more plausible explanations of trial-by-trial changes in MMN amplitude than models representing the two more traditional theories (change detection and adaptation). Our results suggest that the MMN reflects approximate Bayesian learning of sensory regularities, and that the MMN-generating process adjusts a probabilistic model of the environment according to prediction errors.
Pitch Expert: A Problem-Solving System for Kraft Mills

Pitch Expert was developed to make expertise available to mill-site engineers to solve pitch problems in kraft pulp mills. These problems have been estimated to cause losses to the Canadian pulp and paper industry in excess of $100 million per year. The design of the system took into account not only the complexity of the process interactions and the need for accuracy and completeness of recommendations, but the ongoing need for training mill personnel and the requirement that the system be maintainable and expandable without constant involvement of the developers. Pitch Expert is now accessible by modem and the savings achieved through use of the system have covered the development costs within six months of release.
Evaluation of a Localization Training Program for Hearing Impaired Listeners

Objective: To evaluate the effectiveness of a home-based and a laboratory-based localization training program. Design: This study examined the effectiveness of a localization training program on improving the localization ability of 15 participants with a mild-to-moderately severe hearing loss. These participants had worn the study hearing aids in a previous study. The training consisted of laboratory-based training and home-based training. The participants were divided into three groups: a control group, a group that performed the laboratory training first followed by the home training, and a group that completed the home training first followed by the laboratory training. The participants were evaluated before any training (baseline), at 2 weeks, 1 month, 2 months, and 3 months after baseline testing. All training was completed by the second month. The participants only wore the study hearing aids between the second month and the third month. Localization testing and laboratory training were conducted in a sound-treated room with a 360 degree, 12 loudspeaker array. There were three stimuli each randomly presented three times from each loudspeaker (nine test items from each loudspeaker) for a total of 108 items on each test or training trial. The stimuli, including a continuous noise, a telephone ring, and a speech passage “Search for the sound from this speaker” were high-pass filtered above 2000 Hz. The test stimuli had a duration of 300 ms, whereas the training stimuli had five durations (3 s, 2 s, 1 s, 500 ms, and 300 ms) and four back attenuation (−8, −4, −2, and 0 dB re: front presentation) values. All stimuli were presented at 30 dB SL or the most comfortable listening level of the participants. Each participant completed 6 to 8, 2 hr laboratory-based training within a month. The home training required a two-loudspeaker computer system using 30 different sounds of various durations (5) by attenuation (4) combinations. The participants were required to use the home training program for 30 min per day, 5 days per week for 4 weeks. Results: Localization data were evaluated using a 30 degree error criterion. There was a significant difference in localization scores for sounds that originated from the back between baseline and 3 months for the two groups that received training. The performance of the control group remained the same across the 3 month period. Generalization to other stimuli and in the unaided condition was also seen. There were no significant differences in localization performance from other directions between baseline and 3 months. These results indicated that the training program was effective in improving the localization skills of these listeners under the current test set-up. Conclusions: The current study demonstrated that hearing aid wearers can be trained on their front/back localization skills using either laboratory-based or home-based training program. The effectiveness of the training was generalized to other acoustic stimuli and the unaided conditions when the stimulus levels were fixed.
Distinct Genetic and Epigenetic Signatures of Colorectal Cancers According to Ethnic Origin

Background: The outcome of colorectal cancer varies depending on ethnic origin. Egyptian colorectal carcinoma is surprisingly young-age disease with high proportion of rectal and advanced stage cancers. Methods: We characterized 69 sporadic Egyptian colorectal cancers for promoter methylation at 24 tumor suppressor genes, microsatellite instability, and expression of mismatch repair, p53, and β-catenin proteins. Data were compared with 80 Western colorectal carcinoma of sporadic and familial origin from Finland. Results: Egyptian colorectal carcinomas showed significantly higher methylation of the microsatellite stable (MSS) tumors as reflected by the average number of methylated genes per case (P = 0.00002) and tumor suppressor gene methylator phenotype (TSGMP), defined here as methylation of ≥5 genes, (P = 0.0001) compared with the sporadic Western cancers. The TSGMP was associated with advanced stage in the Egyptian cancers (P = 0.0016). Four genes were differentially methylated between Egyptian and Western cases, of which the association of CDKN2B/p15 methylation with Egyptian origin was outstanding (P = 4.83E-10). Egyptian carcinoma also showed significantly lower frequency of nuclear β-catenin localization than the sporadic Western cancers (P = 0.00006) but similar to that of the familial Western subset designated as familial colorectal cancer type X. Conclusions: We show novel pathway in colon carcinogenesis marked by high methylation of MSS cancers, remarkable CDKN2B/p15 methylation, and low frequency of Wnt signaling activation. Impact: Our findings highlight the possible effect of environmental exposures in carcinogenesis through DNA methylation and should have applications in prevention, molecular diagnosis, prognosis, and treatment. Cancer Epidemiol Biomarkers Prev; 21(1); 202–11. ©2011 AACR.
Exploration of an incremental suite of microscopic models for acoustic event monitoring using a robotic sensor network

Simulation is frequently used in the study of multi-agent systems. Unfortunately, in many cases, it is not necessarily clear how faithfully the details of the simulated model represent the behavior of the physical system. Often, the effects of the environment in which the system is to be placed are even neglected entirely. Taking into account the entire system (including interactions with the target environment), establishing a clear hierarchy among multiple levels of modeling not only enhances the fidelity of the individual models, but also emphasizes the tradeoffs inherent in each. Understanding and leveraging the full spectrum of models allows the use of fast, high-level models for exploration in the parameter space, the results of which can be verified on more precise low-level models. Here, we demonstrate the generation of a family of models for a robotic wireless sensor network engaged in an acoustic detection task. Quantitative correspondence is shown between modeling levels and with the physical system.
Regulation of p53 tumor suppressor by Helicobacter pylori in gastric epithelial cells.

BACKGROUND & AIMS Infection with the gastric mucosal pathogen Helicobacter pylori is the strongest identified risk factor for distal gastric cancer. These bacteria colonize a significant part of the world's population. We investigated the molecular mechanisms of p53 regulation in H pylori-infected cells. METHODS Mongolian gerbils were challenged with H pylori and their gastric tissues were analyzed by immunohistochemistry and immunoblotting with p53 antibodies. Gastric epithelial cells were co-cultured with H pylori and the regulation of p53 was assessed by real-time polymerase chain reaction, immunoblotting, immunofluorescence, and cell survival assays. Short hairpin RNA and dominant-negative mutants were used to inhibit activities of Human Double Minute 2 (HDM2) and AKT1 proteins. RESULTS We found that in addition to previously reported up-regulation of p53, H pylori can also negatively regulate p53 by increasing ubiquitination and proteasomal degradation via activation of the serine/threonine kinase AKT1, which phosphorylates and activates the ubiquitin ligase HDM2. These effects were mediated by the bacterial virulence factor CagA; ectopic expression of CagA in gastric epithelial cells increased phosphorylation of HDM2 along with the ubiquitination and proteasomal degradation of p53. The decrease in p53 levels increased survival of gastric epithelial cells that had sustained DNA damage. CONCLUSIONS H pylori is able to inhibit the tumor suppressor p53. H pylori activates AKT1, resulting in phosphorylation and activation of HDM2 and subsequent degradation of p53 in gastric epithelial cells. H pylori-induced dysregulation of p53 is a potential mechanism by which the microorganism increases the risk of gastric cancer in infected individuals.
Sinusoidal Bragg grating based on hybrid metal insulator metal plasmonic waveguide

This is the first report on 3D sinusoidal Bragg grating structure based on hybrid metal insulator metal (HMIM) plasmonic waveguide. The proposed structure has a gradual change in the refractive index rather than an abrupt change providing excellent filtering characteristics. The device is studied at an operating wavelength of 1.55 μm. The results are based on numerical simulations performed using the software CST microwave studio suite. The transmission characteristics show more than 80% transmission in the passband and near zero transmission in the rejection band. The proposed structure reduces the scattering losses and a wide bandgap of 0.387 μm is achieved. The proposed structure has a track length of 3.784 μm for 11 cells, which is very less compared to previous reports allowing large-scale integration of photonic devices. A microcavity is also formed and its resonance is investigated by introducing a defect length of 0.217 μm in the periodic structure. A peak transmission of 50% and a narrow resonance bandwidth of 0.029 μm are achieved at 1.55 μm resonance wavelength. The quality factor which defines the energy stored in the cavity is Q = 53.
Retinal Nerve Fiber Layer and Central Corneal Thickness in Patients with Exfoliation Syndrome

Purpose: To evaluate the retinal nerve fiber layer (RNFL) and central corneal thickness (CCT) in patients with exfoliation syndrome (XFS) Methods: In this comparative case series we measured RNFL thickness and CCT in 30 patients with XFS and in 30 age and sex matched healthy subjects who met the inclusion criteria. Results: Average RNFL in XFS group were significantly thinner than controls (94.36±8.70 µm vs. 100.80±6.68 µm) (P=0.002). In the analysis with regard to quadrants, no statistically significant reduction in RNFL thickness was found between groups. XFS patients and controls did not differ in CCT measurements (522.90±40.71 µm vs. 517.30±30.50 µm) (P=0.549). Conclusion: No significant differences in CCT and RNFL thickness in temporal, nasal, superior and inferior quadrants between XFS and controls were observed. However, average RNFL measurements in eyes with XFS showed lower values.
Frontloading and Intensity of Skilled Home Health Visits: A State of the Science

Frontloading of skilled nursing visits is one way home health providers have attempted to reduce hospital readmissions among skilled home health patients. Upon review of the frontloading evidence, visit intensity emerged as being closely related. This state of the science presents a critique and synthesis of the published empirical evidence related to frontloading and visit intensity. OVID/Medline, PubMed, and Scopus were searched. Seven studies were eligible for inclusion. Further research is required to define frontloading and visit intensity, identify patients most likely to benefit, and to provide a better understanding of how home health agencies can best implement these strategies.
Is tumor size a reliable predictor of histopathological characteristics of renal cell carcinoma?

Objectives: To determine whether smaller tumor size is associated with less-aggressiveness in renal cell carcinoma (RCC). Materials and Methods: Series records of 505 patients diagnosed with RCC were retrospectively reviewed and the data concerning tumor size and pathological information were extracted and analyzed. Results: Five hundred and eight RCCs were identified. The mean tumor size was 5.02 ± 2.70 cm. No correlation was detected between the size of tumor and the histological subtype. The overall nuclear grade distribution was 57.1% and 42.9% for low-grade and high-grade disease, respectively. Each 1 cm increase in tumor size was associated with a significant increase in the odds ratio of high-grade disease by 1.46. 91.1% were found low-stage lesions and the odds ratio for the association of high-stage disease with each 1 cm increase in tumor size was 1.67. Multinomial models revealed that each 1 cm increase in the tumor size was associated with a 35% increase in renal capsule involvement and 66% renal vascular invasion. The cut-off point of tumor size in renal vascular invasion was 5.6 cm. Conclusion: Tumor size is not an independent predictor for the histological subtype of RCC. However, it is closely correlated to histopathological features, with the indications that the greater the tumor size, the more aggressive potential the RCC is.