Miracle-dz/UniFAR-FTUs huggingface dataset:
Adenosine Triphosphate Content in Human Unfertilized Oocytes, Undivided Zygotes and Embryos Unsuitable for Transfer or Cryopreservation

OBJECTIVE: To investigate the relationship between adenosine triphosphate (ATP) content and developmental potential in human oocytes, zygotes and embryos. METHODS: ATP content was determined using a bioluminescence assay in 72 unfertilized oocytes, 26 undivided zygotes and 70 embryos unsuitable for transfer or cryopreservation, obtained from 52 women undergoing in vitro fertilization and embryo transfer (IVF—ET). RESULTS: The mean ATP content increased with development: zygotes had a significantly higher ATP content than oocytes, and embryos had a significantly higher ATP content than both zygotes and oocytes. Within the embryo group, the ATP content was significantly higher in polypronuclear embryos than in inferior embryos with substantial fragmentation. When analysed in relation to IVF—ET outcome, the ATP content of oocytes and embryos from women who became pregnant was significantly higher than in oocytes and embryos from those who did not become pregnant. CONCLUSIONS: Reduced ATP content may be related to fertilization failure, arrested division and abnormal embryonic development. The differences observed in oocyte and embryo ATP content between women who became pregnant and those who did not suggests that mitochondrial function is correlated with individual fertility.
Interpersonal resources and vulnerabilities: the influence of parents and peers on depressive symptoms in relationally victimized adolescents

Adolescence heralds a unique period of vulnerability to depressive symptoms. The current study examined relational victimization, targeting adolescents’ interpersonal relationships, as a unique predictor of depressive symptoms in a broad age range of adolescents. Past research shows that interpersonal resources—particularly emotional support—are negatively related to depression. In this study, the moderating effects of emotional support from mothers, fathers, and peers on the association between relational victimization and depressive symptoms were investigated. As expected, high levels of maternal and peer emotional support buffered the association between relational victimization and depressive symptoms. Emotional support from fathers did not moderate this relationship. Findings also suggest that while support from peers is protective against concurrent depressive symptoms, it can be detrimental to adolescent’s mental health over time. In contrast, maternal emotional support buffers future depressive symptoms associated with past experiences of relational victimization.
Bacillus subtilis Protects Porcine Intestinal Barrier from Deoxynivalenol via Improved Zonula Occludens-1 Expression

Intestinal epithelial cells (IECs) forming the barrier for the first-line of protection are interconnected by tight junction (TJ) proteins. TJ alteration results in impaired barrier function, which causes potentially excessive inflammation leading to intestinal disorders. It has been suggested that toll-like receptor (TLR) 2 ligands and some bacteria enhance epithelial barrier function in humans and mice. However, no such study has yet to be claimed in swine. The aim of the present study was to examine whether Bacillus subtilis could improve barrier integrity and protection against deoxynivalenol (DON)-induced barrier disruption in porcine intestinal epithelial cell line (IPEC-J2). We found that B. subtilis decreased permeability of TJ and improved the expression of zonula occludens (ZO)-1 and occludin during the process of forming TJ. In addition, ZO-1 expression of IPEC-J2 cells treated with B. subtilis was up-regulated against DON-induced damage. In conclusion, B. subtilis may have potential to enhance epithelial barrier function and to prevent the cells from DON-induced barrier dysfunction.
Influence of ionizing radiation on Cobra (Naja haje) and Cerastes cerastes venoms: Toxicological and immunological aspects.

The effect of gamma irradiation (15 KGy) on the Cobra Naja haje and Cerastes cerastes venoms toxicity and immunogenicity was evaluated. Irradiated venoms were at least 28.1% less toxic than non-irradiated venoms. However the antigenic response was not changed as judged by the capacity of irradiated venoms to react with polyvalent antivenom horse serum. The immunodiffusion method showed identity between irradiated and non-irradiated samples. The effect of gamma radiation on some venom enzymes and venoms profile was studied. Proteolytic, phospholipase A2 and hemorrhagic activities were inhibited by irradiation. Irradiation with gamma rays resulted in structural changes in the venoms as indicated by the changes in their molecular weights. The results indicated that irradiation of toxic protein -using suitable dosecan promote significant detoxification but still retaining many of the original antigenic and immuonological properties of native venom.
Implementing process-oriented knowledge management strategies

Despite growing interest about a strategic perspective on knowledge management (KM), there is still a lack of a procedure and methods to guide the implementation of KM strategies. In this paper, we review the current state of practice of KM initiatives and identify four scenarios for potentially successful KM initiatives. The majority of organizations can be described as being a knowledge management starter. In order to improve these KM initiatives and link them to business strategy, we suggest a process‐oriented knowledge management approach as a step to bridge the gap between human‐ and technology‐oriented KM. This approach is outlined with the help of the four levels of intervention: (1) strategy, (2) KM organization and processes, (3) topics/content, and (4) instruments/systems. The definition and implementation of a process‐oriented KM strategy in a large transaction bank will serve as an example to illustrate the application of our approach.
“Popeye muscle” morphology in OBPI elbow flexion contracture

Abstract Background: The pathophysiology of elbow flexion contracture (EFC) in obstetrical brachial plexus injury (OBPI) is not established. In basic science models, neonatal denervation leads to impaired muscle growth. In clinical studies, diminished growth is correlated with extent of denervation, and improved with surgical repair. In EFC, the biceps are clinically short and round vs the contralateral size, termed the “Popeye muscle”. The objective of this study was to determine if the biceps morphology (muscle belly and tendon length) in arms with EFC secondary to OBPI is different vs the contralateral. Methods: This is a retrospective matched-cohort study. Patients with unilateral EFC (>20°) secondary to OBPI were identified (median = 6.6 years, range = 4.7–16.8). A blinded radiologist used computed tomography to measure length of the biceps short head muscle belly, and tendon bilaterally using standardised anatomical landmarks. Results: Twelve patients were analyzed. The biceps muscle belly in the injured arm was shorter in all patients vs contralateral, mean difference = 3.6 cm (80%), p < 0.001. The biceps tendon in the injured arm was longer in all patients vs contralateral, mean difference = 1.13 cm (127%), p < 0.001. The total biceps length in the injured arm was shorter in all patients vs contralateral, mean difference = 2.5 cm (89%), p < 0.001. Conclusions: This is the first human study confirming growth discrepancy of an elbow flexor in EFC. Distinct biceps morphology is demonstrated, with a significantly shorter muscle belly and overall length, but longer tendon vs normal. This is termed the “Popeye muscle” for its irregular morphology. Findings are consistent with impaired limb growth in denervation.
The Constitutive Androstane Receptor and Pregnane X Receptor Function Coordinately to Prevent Bile Acid-induced Hepatotoxicity*

A double null mouse line (2XENKO) lacking the xenobiotic receptors CAR (constitutive androstane receptor) (NR1I3) and PXR (pregnane X receptor) (NR1I2) was generated to study their functions in response to potentially toxic xenobiotic and endobiotic stimuli. Like the single knockouts, the 2XENKO mice are viable and fertile and show no overt phenotypes under normal conditions. As expected, they are completely insensitive to broad range xenobiotic inducers able to activate both receptors, such as clotrimazole and dieldrin. Comparisons of the single and double knockouts reveal specific roles for the two receptors. Thus, PXR does not contribute to the process of acetaminophen hepatotoxicity mediated by CAR, but both receptors contribute to the protective response to the hydrophobic bile acid lithocholic acid (LCA). As previously observed with PXR (Xie, W., Radominska-Pandya, A., Shi, Y., Simon, C. M., Nelson, M. C., Ong, E. S., Waxman, D. J., and Evans, R. M. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 3375-3380), pharmacologic activation of CAR induces multiple LCA detoxifying enzymes and provides strong protection against LCA toxicity. Comparison of their responses to LCA treatment demonstrates that CAR predominantly mediates induction of the cytochrome p450 CYP3A11 and the multidrug resistance-associated protein 3 transporter, whereas PXR is the major regulator of the Na+-dependent organic anion transporter 2. These differential responses may account for the significant sensitivity of the CAR knockouts, but not the PXR knockouts, to an acute LCA dose. Because this sensitivity is not further increased in the 2XENKO mice, CAR may play a primary role in acute responses to this toxic endobiotic. These results define a central role for CAR in LCA detoxification and show that CAR and PXR function coordinately to regulate both xenobiotic and bile acid metabolism.
Hemodynamic comparison between Trifecta and Freestyle valves implanted in small aortic roots. One-year echocardiographic results from a prospective randomized study.

BACKGROUND AND AIM OF THE STUDY Aortic valve replacement in patients with a small aortic root may be associated to high residual gradients. In such patients, both stentless valves and aortic annulus enlargement can reduce these residual gradients. Several studies have reported that Trifecta valves yield very good hemodynamic results. The aim of the present study was to compare the hemodynamic performance of Trifecta vs. Freestyle valves at one year in patients with an aortic annulus ≤ 2.3 cm. METHODS Between September 2011 and September 2013, 40 patients with a native aortic annulus diameter ≤ 2.3 cm and average age of 81 ± 4 years, were randomized to receive either a St-Jude Trifecta stented prosthesis (20 patients) or a Medtronic Freestyle stentless prosthesis (20 patients). RESULTS No differences between Trifecta and Freestyle were found at one year in mean gradient s: 6.1 ± 3 mmHg and 6.6 ± 3 mmHg (p = 0.796); effective ori fice area: 1.82 ± 0.3 mmHg and 1.76 ± 0.4 mmHg (p = 0.676) or regression of left ventricular mass: - 25% ± 14 vs. -19% ± 16 (p = 0.204), respectively. Only moderate patient -pro sthesis mismatch was found, which affected 3 patient s in each group. CONCLUSION At one year both stentless and stented prostheses yielded comparable hemodynamic results. These data suggest that Trifecta implantation is a valid means of avoiding patient -prosthesis mismatch in aortic valve replacement in elderly patients with a small native aortic annulus.
Communication and Collaboration, Satisfaction, Equity, and Autonomy in Blended Learning Environments: A Case from Turkey

Research studies conducted in the area of e-learning and blended learning lead to the emergence of new concepts, which directly influence quality. Among these concepts, the ones taken into consideration for this research study are as follows: communication and collaboration, satisfaction, equity, and autonomy. The perceptions of students taking blended ICT courses in a private university in Turkey were gathered through an Online Learning Environment Survey (OLES). Additionally, the suggestions of instructors for improving the quality of blended learning were obtained through a focus group interview. The findings of this study show that the perceived communication, collaboration, and satisfaction levels of students vary according to their levels of computer and Internet literacy. Also, there are differences in the students’ satisfaction levels based on gender. The majority of students revealed that they considered themselves to be autonomous and equal in the blended learning environment. The findings of this research study, together with our review of recent literature, lead us to conclude that there are four major areas (containing several factors) that must be considered when developing a high-quality blended learning environment: technology, instructors, students, and pedagogy. Based on the findings, some practical suggestions for transforming traditional courses into blended ones are also offered at the end of the research study. Finally, suggestions for the future are provided.
Psychometric properties of a Dutch short form of the Arthritis Impact Measurement Scales 2 (Dutch-AIMS2-SF).

OBJECTIVE To evaluate the reliability and validity of a Dutch version of the Arthritis Impact Measurement Scales 2 short form (AIMS2-SF) and examine the agreement between the AIMS2 and AIMS2-SF in rheumatoid arthritis (RA) patients. METHODS Data were collected from 587 RA patients from three studies. Patients completed the Dutch-AIMS2, Modified Health Assessment Questionnaire (M-HAQ), and Visual Analogue Scale for pain (VAS-pain), and clinical data were collected to calculate the Disease Activity Score 28 (DAS28). Short-form component scores were calculated from the AIMS2 long-form data. In addition, a Modified Symptom component score was calculated by replacing item 42 with item 38 as was suggested by Haavardsholm et al. [7] for the Norwegian version. RESULTS The internal consistency of the Physical, Symptom and Affect components was good (Cronbach's alpha= 0.75-0.87), moderate for the Role component (alpha=0.62) but rather low for the Social Interaction (0.51) component. Replacing item 33 with item 31 of the long-form AIMS2 increased internal consistency for the Social Interaction component to 0.63. Test-retest reliability of the AIMS2-SF components was high (intraclass correlation coefficients >0.70). Mean scores of the AIMS2-SF were generally close to those from the AIMS2, but the limits of agreement were rather wide. Both the Modified Symptom and Modified Social Interaction components showed better agreement than the original short-form components. Plots of differences between AIMS2 and AIMS2-SF against the mean of the two scores for the five components showed that the differences varied over the range of the measurements. Factor analysis confirmed the three-factor structure, with a physical, psychological and social dimension that has been found for the Dutch-AIMS2 long form. Correlations of the AIMS2-SF components with M-HAQ total score, functional class, VAS-pain and DAS28 were very similar to the correlations for the original AIMS2. CONCLUSION The Dutch-AIMS2-SF, with Modified Symptom and Social Interaction components has good psychometric properties, similar to those of the Dutch-AIMS2 long form.
Lost and unfulfilled relationships behind emotional loneliness in old age

ABSTRACT Using a qualitative approach, this article examines how the experiences of emotional loneliness are embedded in the everyday lives and relationships of older adults. Ten in-depth interviews were conducted in 2010 with older people who reported feeling lonely, often or all the time, during a cohort study in southern Finland. The research reveals the multifaceted nature of loneliness and its causes. Behind emotional loneliness, we identified lost and unfulfilled relationships, involving the loss or lack of a partner, the absence of a meaningful friendship, complex parenthood and troubling childhood experiences. Most of the interviewees have faced loneliness that only began in old age, but for some, loneliness has been present for nearly a lifetime.
Soft Sensing of Polypropylene Melt Index Based on Improved RBF Network

In view of the problem in the practical application of radial basis function (RBF) network, such as the number of nodes in the hidden layer and the parameters (w, c, and σ) are hard to determine, an improved particle swarm optimization (PSO) algorithm which makes use of the advantages of PSO algorithm and genetic algorithm (GA) is proposed, and then optimize the RBF network model with the new algorithm for soft sensing of polypropylene melt index. Simulation results show that the new method can improve the network's training speed and predictive precision effectively, and is more suitable for soft sensing of polypropylene melt index.
Some Correlates of Romantic Love

Statistical analysis of the questionnaire responses of 1,079 white college students reveals substantial differences in the romantic orientation of males and females. Females are found to have more romantic experiences than males, even when age is controlled. The difference is reduced, however, with "closeness to marriage"; in fact, the reported figure for females undergoes a reversal. Sociologically, this reversal phenomenon is attributed to a directedness in female romantic orientation for which there appears to be no male counterpart. The implications of this sex difference are discussed in terms of current mate selection theories.
Being a mentor: novice teachers’ mentors’ conceptions of mentoring prior to training

This study deals with novice teachers’ mentors’ conceptions of mentoring prior to their mentoring training. In Israel, all novice teachers have to be supported and assessed by a mentor during their first year of teaching. The aim of this study was to elicit from prospective mentors their own conception of professional mentoring, as a basis for developing mentor training courses. We administered a questionnaire to 170 experienced teachers who had just started taking mentor courses in Israel. The open-ended questions therein included five category components: the mentor’s spheres of responsibility; content addressed in mentoring work; qualities required in mentors; professional skills required for mentoring; and indicators of successful mentoring. The findings mapped perceptions of the role of mentors but also revealed missing elements. The concluding discussion of the findings provides insight into the planning of training programs for mentors of novice teachers, thereby optimizing the training process and the professional abilities of their graduates.
Political Ethos Revisited

An effort to test the existence and correlates of the “unitarist” and “individualist” political ethos (first discussed in City Politics under the labels “middle-class Anglo-Saxon ethos” and “immigrant ethos”) in a sample of 1,059 mostly male Boston homeowners reveals that about one fifth of the respondents have one or the other ethos when defined by two sets of attitudes and about one eighth have one or the other when defined by three sets of attitudes. In general, the respondents displaying each attitude or two or more attitudes in the predicted combinations have the predicted ethnic, religious, income, and educational attributes. Jewish voters, however, are less likely than predicted to have the good government attitude, whereas Irish and Polish respondents are more likely to have it. Upper-income Yankees were strongly unitarist as defined by all three attitudes.
Computationally sound mechanized proofs for basic and public-key Kerberos

We present a computationally sound mechanized analysis of Kerberos 5, both with and without its public-key extension PKINIT. We prove authentication and key secrecy properties using the prover CryptoVerif, which works directly in the computational model; these are the first mechanical proofs of a full industrial protocol at the computational level. We also generalize the notion of key usability and use CryptoVerif to prove that this definition is satisfied by keys in Kerberos.
Assessment of carcinogenic risk from personal exposure to benzo(a)pyrene in the Total Human Environmental Exposure Study (THEES).

The Total Human Environmental Exposure Study (THEES) was an investigation of multimedia exposure to the ubiquitous environmental carcinogen, benzo(a)pyrene (BaP). The three-phase study was conducted in Phillipsburg, New Jersey and involved the participation of 14-15 individuals (8-10 homes) during each 14-day monitoring period. Microenvironmental sampling of air, food, water and soil indicated that environmental exposure to BaP was primarily through air and food. Exposure and risk estimates were, therefore, based on the results of personal monitoring of breathing zone air and prepared food samples. Based on a comparison of the range and magnitude of inhalation and dietary BaP exposures, food ingestion was clearly the predominant exposure to pathway. The relative contributions of other potential sources of community exposure to BaP (e.g., soil and drinking water ingestion) were also assessed. The excess cancer risk estimates for food ingestion were consistently greater than those for personal air, reflecting both the predominantly higher BaP exposures through the diet and the higher carcinogenic potency value for oral exposure. Overall, the total lifetime risk from personal exposure to BaP for nonsmokers in the community was estimated at 10(-5). In identifying risk reduction options, it is important to account for the observation that personal activities, lifestyle, and diet strongly influenced individual exposures to BaP.
Cholinergic Atrial Fibrillation in a Computer Model of a Two-Dimensional Sheet of Canine Atrial Cells With Realistic Ionic Properties

Classical concepts of atrial fibrillation (AF) have been rooted in Moe’s multiple-wavelet hypothesis and simple cellular-automaton computer model. Recent experimental work has raised questions about the multiple-wavelet mechanism, suggesting a discrete “driver region” underlying AF. We reexplored the theoretical basis for AF with a 2-dimensional computer model of a 5×10-cm sheet of atrial cells with realistic ionic and coupling properties. Vagal actions were formulated based on patch-clamp studies of acetylcholine (ACh) effects. In control, a single extrastimulus resulted in a highly meandering unstable spiral wave. Simulated electrograms showed fibrillatory activity, with a dominant frequency (DF, 6.5 Hz) that correlated with the mean rate. Uniform ACh reduced core meander of the spiral wave by ≈70% (as measured by the standard deviation of spiral-wave tip position) and accelerated the DF to 17.0 Hz. Simulated vagally induced refractoriness heterogeneity caused wavefront breakup as accelerated reentrant activity in regions of short refractoriness impinged on regions unable to respond in a 1:1 fashion because of longer refractoriness. In 7 simulations spanning the range of conditions giving sustained AF, 5 were maintained by single dominant spiral waves. On average, 3.0±1.3 wavelets were present (range, 1 to 7). Most wavelets were short-lived and did not contribute to AF maintenance. In contrast to predictions of the multiple-wavelet hypothesis, but in agreement with recent experimental evidence, our model indicates that AF can result from relatively stable primary spiral-wave generators and is significantly organized. Our results suggest that vagal AF may arise from ACh-induced stabilization of the primary spiral-wave generator and disorganization of the heterogeneous tissue response. The full text of this article is available at http://www.circresaha. org.