Miracle-dz/UniFAR-FTUs huggingface dataset:
Detection and properties of the human proliferative monocyte subpopulation

Peripheral blood monocyte subpopulations have been reported and can give rise to diverse, differentiated phenotypes. A subpopulation(s) of human monocytes can proliferate in vitro in response to macrophage‐colony stimulating factor (M‐CSF; or CSF‐1). This population, termed the proliferative monocyte (PM), is presumably less mature than other monocytes; however, it has not been defined further. Previous studies monitoring the frequency of the slowly cycling PM from different donors indicated that the assay for their reproducible measurement required improvement. We demonstrate that for optimal PM detection, high 5‐bromo‐2′‐deoxyuridine concentrations are required over a delayed and wide time‐frame. Surface marker phenotyping by flow cytometry showed that freshly isolated PM are CD14+ and could be distinguished from two other human monocyte subpopulations, namely, the CD14loCD16+ and CD14loCD64– subsets. PM express relatively high levels of CD64 and CD33 but have relatively low CD13 expression; they are also c‐Fms+ and human leukocyte antigen‐DR+. Labeling with carboxyfluorescein diacetate succinimidyl ester (CFSE) enabled the estimation of the number of PM divisions over time. Following CFSE labeling and culture, PM were sorted from the nonproliferating population and shown to have a distinctive, spindle‐shaped morphology and higher capacity to form multinucleated, tartrate‐resistant acid phosphatase+ cells in the presence of M‐CSF and receptor activator of nuclear factor‐κB ligand. The phenotype and properties of the PM subpopulation were examined as a prelude to determining its role in disease using methods that can be applied to clarify human monocyte heterogeneity.
Temporal Profile and Mechanisms of the Prompt Sympathoexcitation following Coronary Ligation in Wistar Rats

Our aim was to assess the timing and mechanisms of the sympathoexcitation that occurs immediately after coronary ligation. We recorded thoracic sympathetic (tSNA) and phrenic activities, heart rate (HR) and perfusion pressure in Wistar rats subjected to either ligation of the left anterior descending coronary artery (LAD) or Sham operated in the working heart-brainstem preparation. Thirty minutes after LAD ligation, tSNA had increased (basal: 2.5±0.2 µV, 30 min: 3.5±0.3 µV), being even higher at 60 min (5.2±0.5 µV, P<0.01); while no change was observed in Sham animals. HR increased significantly 45 min after LAD (P<0.01). Sixty minutes after LAD ligation, there was: (i) an augmented peripheral chemoreflex – greater sympathoexcitatory response (50, 45 and 27% of increase to 25, 50 and 75 µL injections of NaCN 0.03%, respectively, when compared to Sham, P<0.01); (ii) an elevated pressor response (32±1 versus 23±1 mmHg in Sham, P<0.01) and a reduced baroreflex sympathetic gain (1.3±0.1 versus Sham 2.0±0.1%.mmHg−1, P<0.01) to phenylephrine injection; (iii) an elevated cardiac sympathetic tone (ΔHR after atenolol: −108±8 versus −82±7 bpm in Sham, P<0.05). In contrast, no changes were observed in cardiac vagal tone and bradycardic response to both baroreflex and chemoreflex between LAD and Sham groups. The immediate sympathoexcitatory response in LAD rats was dependent on an excitatory spinal sympathetic cardiocardiac reflex, whereas at 3 h an angiotensin II type 1 receptor mechanism was essential since Losartan curbed the response by 34% relative to LAD rats administered saline (P<0.05). A spinal reflex appears key to the immediate sympathoexcitatory response after coronary ligation. Therefore, the sympathoexcitatory response seems to be maintained by an angiotensinergic mechanism and concomitant augmentation of sympathoexcitatory reflexes.
Seroprevalence and incidence of hepatitis E virus among blood donors: A review

Hepatitis E virus (HEV) is an RNA virus with 4 main genotypes. HEV‐1 and HEV‐2 infect solely humans, while HEV‐3 and HEV‐4 infect humans and various animals such as pigs, deer, and rabbits. HEV‐5 and HEV‐6 infect mainly wild boar. Recently, new genotypes, known as HEV‐7 and HEV‐8, were found to infect camels and humans. HEV is globally distributed into different epidemiological patterns based on socioeconomic factors and ecology. Although HEV is mainly transmitted through the fecal‐oral route, it was also recognized as a transfusion‐transmitted virus. Transmission through blood donation was documented worldwide with rising annual observations, accounting for more than 2.5% of all transmissions. HEV infection is usually asymptomatic or subclinical in immunocompetent individuals, so it remains questionable whether there is an urgent need to screen for HEV prior to blood transfusion. Moreover, recent studies conducted in the Middle East and North Africa (MENA) region indicate that HEV is highly endemic. Here, we provide a review on HEV epidemiology, transmission, and laboratory diagnosis, giving special emphasis to the newly discovered genotypes, HEV‐7 and HEV‐8. Furthermore, we underscore the findings of recent HEV seroprevalence and viremia studies among blood donors worldwide. We also shed light on similar studies performed among blood donors in the MENA region.
Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant

Camptothecin (CPT) analogues and derivatives serve as a novel class of effective anticancer agents that exert their action against DNA topoisomerase I. This paper presents procedures for the rapid, high frequency regeneration of a camptothecin producing plant, Ophiorrhiza prostrata D. Don from leaf and internode explants via shoot organogenesis. The concentrations of plant growth regulators and explant types exhibited discrete roles in the efficacy of shoot induction. N 6 -benzyladenine (BA) was the most effective cytokinin for the induction of shoots. Murashige and Skoog (MS) medium with 8.87 µM BA and 2.46 µM indole-3-butyric acid (IBA) yielded the highest number of shoots from leaf and internode explants (76.0 and 90.8 shoots respectively). In the case of leaf explants, explants from the proximal end produced a higher number of shoots than those from the mid and distal end. Leaf and internode explants cultured on MS medium supplemented with α-naphthaleneacetic acid (NAA) and BA developed shoots, calli and roots. Calli subcultured onto medium supplemented with 8.87 µM BA and 2.46 µM IBA developed a mean of 20.1 shoots within 40 days. Excision and culture of internode and proximal leaf explants from the established cultures on MS basal medium significantly enhanced the number of shoots and yielded a mean of 18.3 and 13.7 shoots respectively within 40 days. Histological examination of leaf explants showed that the shoots were of sub-epidermal origin, confined to the sub-epidermal cells above the vascular traces. Shoots cultured on half-strength MS basal medium with 10.74 µM NAA and 2.32 µM Kn produced a mean of 48.2 roots per shoot. Direct transfer of rootless healthy shoots showed a 50% survival rate, whilst it was 100 percent in the case of in vitro rooted shoots.
Guided Wave Acoustic Emission from Fatigue Crack Growth in Aluminium Plate

Acoustic emission (AE) testing is an increasingly popular technique used for nondestructive evaluation (NDE). It has been used to detect and locate defects such as fatigue cracks in real structures. The monitoring of fatigue cracks in plate-like structures is critical for aerospace industries. Much research has been conducted to characterize and provide quantitative understanding of the source of emission on small specimens. It is difficult to extend these results to real structures as most of the experiments are restricted by the geometric effects from the specimens. The aim of this work is to provide a characterization of elastic waves emanating from fatigue cracks in plate-like structures. Fatigue crack growth is initiated in large 6082 T6 aluminium alloy plate specimens subjected to fatigue loading in the laboratory. A large specimen is utilized to eliminate multiple reflections from edges. The signals were recorded using both resonant and nonresonant transducers attached to the surface of the alloy specimens. The distances between the damage feature and sensors are located far enough apart in order to obtain good separation of guided-wave modes. Large numbers of AE signals are detected with active fatigue crack propagation during the experiment. Analysis of experimental results from multiple crack growth events are used to characterize the elastic waves. Experimental results are compared with finite element predictions to examine the mechanism of AE generation at the crack tip.
Mortality in vegetarians and comparable nonvegetarians in the United Kingdom123

Background: Vegetarians and others who do not eat meat have been observed to have lower incidence rates than meat eaters of some chronic diseases, but it is unclear whether this translates into lower mortality. Objective: The purpose of this study was to describe mortality in vegetarians and comparable nonvegetarians in a large United Kingdom cohort. Design: The study involved a pooled analysis of data from 2 prospective studies that included 60,310 persons living in the United Kingdom, comprising 18,431 regular meat eaters (who ate meat ≥5 times/wk on average), 13,039 low (less-frequent) meat eaters, 8516 fish eaters (who ate fish but not meat), and 20,324 vegetarians (including 2228 vegans who did not eat any animal foods). Mortality by diet group for each of 18 common causes of death was estimated with the use of Cox proportional hazards models. Results: There were 5294 deaths before age 90 in >1 million y of follow-up. There was no significant difference in overall (all-cause) mortality between the diet groups: HRs in low meat eaters, fish eaters, and vegetarians compared with regular meat eaters were 0.93 (95% CI: 0.86, 1.00), 0.96 (95% CI: 0.86, 1.06), and 1.02 (95% CI: 0.94, 1.10), respectively; P-heterogeneity of risks = 0.082. There were significant differences in risk compared with regular meat eaters for deaths from circulatory disease [higher in fish eaters (HR: 1.22; 95% CI: 1.02, 1.46)]; malignant cancer [lower in fish eaters (HR: 0.82; 95% CI: 0.70, 0.97)], including pancreatic cancer [lower in low meat eaters and vegetarians (HR: 0.55; 95% CI: 0.36, 0.86 and HR: 0.48; 95% CI: 0.28, 0.82, respectively)] and cancers of the lymphatic/hematopoietic tissue [lower in vegetarians (HR: 0.50; 95% CI: 0.32, 0.79)]; respiratory disease [lower in low meat eaters (HR: 0.70; 95% CI: 0.53, 0.92)]; and all other causes [lower in low meat eaters (HR: 0.74; 95% CI: 0.56, 0.99)]. Further adjustment for body mass index left these associations largely unchanged. Conclusions: United Kingdom–based vegetarians and comparable nonvegetarians have similar all-cause mortality. Differences found for specific causes of death merit further investigation.
Increasing collaboration and participation in smart city governance: a cross-case analysis of smart city initiatives

ABSTRACT This study addresses the concept of smart governance in the context of smart cities, with a focus on analyzing the phenomenon of smart collaboration. Relying on the existing collaboration and participation concepts in the smart city domain, an empirical analysis was undertaken of how ICT can promote collaborative governance and increase the participation and engagement in government. The multiple case studies focus on three cities in Brazil that run municipal operations centers in an effort to “become smarter”: Rio de Janeiro, Porto Alegre, and Belo Horizonte. Interviews with directors, managers, and technicians shed light on the contribution that ICT makes in promoting an environment of collaboration in the government. The findings have revealed that ICT has an important role in supporting information sharing and integration between government agencies and external stakeholders, including citizens, especially in developing countries.
Gene network analysis reveals a novel 22-gene signature of carbon metabolism in hepatocellular carcinoma

Although much progress has been made in understanding cancer cellular metabolism adaptation, the co-regulations between genes of metabolism and cancer pathways and their interactions remain poorly characterized. Here, we applied gene co-expression network analysis to 1509 metabolic gene expression data generated from 120 HCC and 180 non-tumor human liver tissues by microarray. Our analyses reveal that metabolism genes can be classified into different co-expression modules based on their associations with HCC related traits. The co-regulation mechanism of the carbon metabolism genes in normal liver tissues was interrupted during the processes of carcinogenesis. In parallel, we performed RNAseq analysis of HCC and non-tumor human liver tissues, and identified a unique 22-carbon-metabolism-gene-signature of increased expression. This gene signature was further verified in multiple microarray data sets, and its prognostic value was also proven by HCC patients' survival data from TCGA. Additionally, the tumorigenic function of two representative genes, CS and ACSS1, were validated experimentally by cell growth and spheroid formation assays. The current study provides evidence for the reprogramming of the co-regulation network between carbon metabolism and cancer pathway genes in HCC. In addition, this study also reveals a unique 22-carbon-metabolism-gene-expression-signature in HCC. Strategies targeting these genes may represent new therapeutic approaches for HCC treatment.
A Whole Other Story

Conflict narratives, having at their core the belief that the ingroup suffered more than the outgroup (competitive victimhood), are key in maintaining conflicts. Three experiments conducted with Jewish Israelis (Study 1), Turkish Kurds (Study 2), and Americans (Study 3) tested whether conflict narratives can reduce conflict. Studies 1 and 3 showed that people respond to inclusive victimhood narratives that emphasize both ingroup and outgroup suffering with a reduction in competitive victimhood and, in turn, reduced support for aggressive policies—but only when people were relatively less concerned that acknowledgment of outgroup suffering might risk loss of third-party support. Study 2 further found that inclusive narratives reduce conflict for low-power groups, yet without being moderated by concern. Together, these studies show that inclusive victimhood narratives can reduce conflict when people are not concerned about losing third-party support. The important role of third parties in conflict resolution is discussed.
Field Cage Studies and Progressive Evaluation of Genetically-Engineered Mosquitoes

Background A genetically-engineered strain of the dengue mosquito vector Aedes aegypti, designated OX3604C, was evaluated in large outdoor cage trials for its potential to improve dengue prevention efforts by inducing population suppression. OX3604C is engineered with a repressible genetic construct that causes a female-specific flightless phenotype. Wild-type females that mate with homozygous OX3604C males will not produce reproductive female offspring. Weekly introductions of OX3604C males eliminated all three targeted Ae. aegypti populations after 10–20 weeks in a previous laboratory cage experiment. As part of the phased, progressive evaluation of this technology, we carried out an assessment in large outdoor field enclosures in dengue endemic southern Mexico. Methodology/Principal Findings OX3604C males were introduced weekly into field cages containing stable target populations, initially at 10∶1 ratios. Statistically significant target population decreases were detected in 4 of 5 treatment cages after 17 weeks, but none of the treatment populations were eliminated. Mating competitiveness experiments, carried out to explore the discrepancy between lab and field cage results revealed a maximum mating disadvantage of up 59.1% for OX3604C males, which accounted for a significant part of the 97% fitness cost predicted by a mathematical model to be necessary to produce the field cage results. Conclusions/Significance Our results indicate that OX3604C may not be effective in large-scale releases. A strain with the same transgene that is not encumbered by a large mating disadvantage, however, could have improved prospects for dengue prevention. Insights from large outdoor cage experiments may provide an important part of the progressive, stepwise evaluation of genetically-engineered mosquitoes.
Melatonin Differentially Modulates NF-кB Expression in Breast and Liver Cancer Cells.

BACKGROUND NF-kB (nuclear factor kappa B) is a transcription factor composed of two subunits, p50 and p65, which plays a key role in the inflammatory process. Melatonin has oncostatic, antiangiogenic and antimetastatic properties, and some recent studies have indicated an inhibitory effect of melatonin on NF-kB in some types of cancer. This work aims to investigate the effects of melatonin treatment on the expression of NFkB in breast and liver cancer models. METHOD The breast cancer xenographic model was performed using female Balb/c nude athymic mice injected with MDA-MB-231 cells. The animals were treated with 40 mg/Kg of melatonin for 21 days. Volume of the tumors was measured with a digital caliper. Hepatocarcinoma model was developed by using the HepG2 cells in vitro, treated with 1 mM melatonin for 24 h. The expression of NF-kB protein was verified by immunohistochemistry and immunocytochemistry and quantified by optical densitometry, in vivo study and in vitro study, respectively. NF-kB gene expression was performed by quantitative RT-PCR. RESULTS The breast cancer xenografts nude mice treated with melatonin showed reduced tumor size (P=0.0022). There was a decrease in NF-kB protein staining (P=0.0027) and gene expression (P=0.0185) in mice treated with melatonin. The opposite results were observed for the hepatocarcinoma model. HepG2 cells treated with melatonin showed an increase in the NF-kB immunostaining when compared to control cells (P=0.0042). CONCLUSION Our results indicated that the treatment with melatonin was able to decrease both gene and protein expressions of NF-kB in breast cancer cells and, conversely, increase the transcription factor protein expression in hepatocarcinoma cells. These data highlighted a double role in the expression of NF-kB, depending on the cell type. Further studies are needed to better elucidate the action of melatonin in NF-kB, since this transcription factor acts on different signaling pathways that are fundamental for carcinogenesis.
Entrepreneurs’ decision making under different levels of uncertainty: the role of emotions

Purpose – The purpose of this paper is to investigate entrepreneurs’ investment decisions under uncertainty in continued investments where the authors test the role of emotions to continue or discontinue the investment. Design/methodology/approach – A conjoint analysis is carried out on 101 entrepreneurs’ 3,232 investment decisions. The entrepreneurs were provided with a scenario of an investment where the dependent variable was the entrepreneur's propensity to allocate further resources to the described investment. They assessed their willingness to allocate further resources to the investment on a seven-point Likert-type scale. The independent variables in the experiment were the experienced emotions of the entrepreneur each of which was described by the two levels of high and low. Findings – It was found that self-confidence, challenge, and hope increase the propensity to continue investments as do increased level of uncertainty. Embarrassment and strain do not increase this propensity, however, high u.
Ten-year seawater tests on aluminum

Seven aluminum alloys and panels of high-purity aluminum were exposed for ten years in seawater at Wrightsville Beach, N. C. Replicate 4 by 12-in. panels of each material were exposed in half-tide immersion and full immersion. Removals in triplicate were made at the end of one-, two-, five-, and ten-year exposures. Other than the 1199 aluminum (99.99 percent purity), seven aluminum-magnesium alloys (5000 series) were tested. Panels were degreased prior to exposure and were cleaned ultrasonically after exposure in a chromic-phosphoric acid solution. After depth of pitting was measured, tension specimens were cut from each panel, and the mechanical properties determined. Panels were heavily fouled with barnacles and other marine growth for all exposure periods. The fouling had little apparent effect on the pitting depth of the alumina n in tidal immersion, but generated heavy etching on some alloys during the 5 to 10 year exposure interval. Little change in tensile properties after ten years' exposure was noted for any of the test alloys. Tensile losses reported for several alloys after full immersion include: 5086-0 (3.7 percent), 5154-H38 (5.1 percent), and 5457-H34 (5.2 percent). The high-purity 1199 and alloys 5154-H38, 5456-0, and 5456-H321 showed losses of 16 to 27 percent in elongation while the change in elongation for the 5086-0 was a decrease of about 6 percent. Confirming other work, we found the corrosion rates to be greater for the full seawater immersion than for the tidal immersion. The lowest ten-year corrosion rate in the half-tide location was 0.014 mpy (mils per year) and the 5456-0, and the highest rate for the aluminum-magnesium 5000 series was 0.051 mpy for the 5456-H321 alloy. In full-immersion tests, the lowest rate was 0.064. For comparison, the rates for the 1199 alloy were 0.036 mpy in tidal and 0.061 mpy in full immersion. Corrosion weight losses and depth of pitting had reduced rates of growth over the interval between five years and ten years. Maximum depth of pitting for an alloy was generally at least four times the magnitude of the average of the twenty deepest pits. The deepest pit found for any alloy after ten years was 72.0 mils for 5456-H321 in half-tide immersion and 131.0 mils for the same alloy (0 temper) in full immersion. Least apparent pitting depths occurred on alloys 5154-H38, 5457-H34, 5086-0 and high-purity 1199 where heavy etching tended to hide pits on the ten-year full-immersion panels.
Culture supernatant produced by Lactobacillus kefiri from kefir inhibits the growth of Cronobacter sakazakii

Cronobacter sakazakii is a life-threatening foodborne pathogen found in powdered infant formula and dairy products. Kefir is a dairy probiotic product and its antimicrobial activity against C. sakazakii was reported in our previous study. To identify key microorganisms that mediate growth suppression, we tested the antimicrobial activity of culture supernatants derived from lactic acid bacteria found in kefir. Lactobacillus kefiri DH5, L. kefiranofaciens DH101, and B ifidobacterium longum 720 (a commercial probiotic strain that served as a positive control) all significantly inhibited the growth of C. sakazakii ATCC 29544, delaying the initiation of exponential growth from 3 to 9 h in the nutrient broth. Among them, L. kefiri DH5 exerted the strongest antimicrobial effects against C. sakazakii, showing bactericidal effect at the addition of 300 µl of supernatant in 1 ml of nutrient broth. Interestingly, the supernatant of L. kefiri DH5 has higher pH and lower titrable acidity than that of L. kefiranofaciens DH101, suggesting metabolites produced by heterofermentation of L. kefiri acted more effectively to antagonise the growth of C. sakazakii. In addition, the supernatant of L. kefiri DH5 induced the leakage of cytoplasmic materials including nucleic acid and proteins, suggesting L. kefiri DH5 disrupted the cellular membrane integrity of C. sakazakii. Considering that pH neutralisation reduced the L. kefiri-dependent growth suppression, it is inferred that this activity is mainly due to organic acids produced during the fermentation process.