Miracle-dz/UniFAR-FTUs huggingface dataset:
Cave monitoring in the Béke and Baradla caves (Northeastern Hungary): implications for the conditions for the formation cave carbonates

: In order to use speleothems in the reconstruction of past climate and environmental changes it is necessary to understand the environmental and hydrological processes that determine the physico-chemical conditions of carbonate precipitation and hence speleothem formation. Therefore, in this study an extended monitoring program was conducted in the Béke and Baradla caves located in the Aggtelek region (Northeastern Hungary). The studied caves are rich in speleothem and flowstone occurrences with great potential for paleoclimatology studies. The monitoring activity included measurements of atmospheric and cave temperatures, CO 2 concentration in cave air, as well as chemical and isotopic compositions of water samples (drip water, precipitation) and in situ carbonate precipitates. The hydrogen and oxygen isotope compositions of drip waters showed no seasonal variation at any of the collection sites, indicating a well-mixed karstic aquifer. This implies that the isotopic compositions of local speleothems were able to record multiannual isotopic changes inherited from stable isotopes in the drip water. CO 2 concentration showed seasonality (high values in summer and low values in winter) in both caves, likely affecting carbonate precipitation or corrosion and consequently stalagmite growth. Systematic variations among Mg/Ca and Sr/Ca, Na/Ca, and Si/Ca element ratios were detected in the drip water suggesting Prior Calcite Precipitation (PCP). As PCP is characteristic of periods of reduced infiltration during drier weather conditions, the variations in drip water chemistry and drip rates indicate that the hydrological conditions also varied significantly during the studied period. This hydrological variability appears to affect not only trace element composition but also the isotopic composition of modern carbonate precipitates. In summary, these findings imply that the speleothems from the studied caves were able to record the hydrological changes resulting from alternating wet and dry periods, and therefore the geochemical data can be used to reconstruct past climate and environmental changes. Béke, Baradla, cave monitoring, PCP, stable isotope, fresh carbonate
Prosody, emotions, and... 'whatever'

Weexaminetheroleofprosodyincueingascaleofnegative� meaningsassociatedwiththeuseof� whatever. �Theanalysisof� acorpusofelicitedexamplesshowsthatthemorenegative� thetoken,�themorelikelyitistohaveanadditionalpitch� accent,�extendedduration,�andexpandedpitchrangeonthe� firstsyllable. �Thesefindingsareanalyzedasalinkbetween� pragmaticmeaningandthestrengthoftheprosodicboundary� betweenthefirsttwosyllables�( what#ever). �Theresultsof� perceptionexperimentsshowthattheprosodyofwhatever� itselfisasystematiccueforthedegreeofnegative� connotationassociatedwiththeutteranceinwhich� whatever� occurs. � Potentialapplicationsofthisresultforspoken� dialoguesystemsandsynthesisofemotionalspeechare� discussed. �
Predicting mobility outcome one year after stroke: a prospective cohort study.

OBJECTIVE To develop a prognostic model to predict mobility outcome one year post-stroke. DESIGN Prospective cohort study in patients with a first-ever stroke admitted for inpatient rehabilitation. PATIENTS A total of 217 patients with stroke (mean age 58 years) following inpatient rehabilitation in 4 rehabilitation centres across the Netherlands. METHODS Mobility was measured using the Rivermead Mobility Index at one year poststroke. Included independent variables were: patient and stroke characteristics, functional status, urinary incontinence, sitting balance, motor and cognitive function. Univariate and multivariate linear regression analyses were performed in a model-developing set (n=174) and the model was validated in cross-validation set (n=43). RESULTS Total Rivermead Mobility Index score at one year post-stroke was predicted by functional status, sitting balance, time between stroke onset and measurement, and age. The derived model predicted 48% of the variance, while validation in the cross-validation set resulted in an adjusted R(2) of 0.47. CONCLUSION The present prospective study shows that outcome of mobility one year after stroke can be predicted validly by including functional status, sitting balance, moment of admission to the rehabilitation centre after stroke onset and age.
CSB interacts with SNM1A and promotes DNA interstrand crosslink processing

Cockayne syndrome (CS) is a premature aging disorder characterized by photosensitivity, impaired development and multisystem progressive degeneration, and consists of two strict complementation groups, A and B. Using a yeast two-hybrid approach, we identified the 5′-3′ exonuclease SNM1A as one of four strong interacting partners of CSB. This direct interaction was confirmed using purified recombinant proteins—with CSB able to modulate the exonuclease activity of SNM1A on oligonucleotide substrates in vitro—and the two proteins were shown to exist in a common complex in human cell extracts. CSB and SNM1A were also found, using fluorescently tagged proteins in combination with confocal microscopy and laser microirradiation, to be recruited to localized trioxsalen-induced ICL damage in human cells, with accumulation being suppressed by transcription inhibition. Moreover, SNM1A recruitment was significantly reduced in CSB-deficient cells, suggesting coordination between the two proteins in vivo. CSB-deficient neural cells exhibited increased sensitivity to DNA crosslinking agents, particularly, in a non-cycling, differentiated state, as well as delayed ICL processing as revealed by a modified Comet assay and γ-H2AX foci persistence. The results indicate that CSB coordinates the resolution of ICLs, possibly in a transcription-associated repair mechanism involving SNM1A, and that defects in the process could contribute to the post-mitotic degenerative pathologies associated with CS.
A Genetic Analysis of Current Medication Use in the UK Biobank

Genomics has been forecasted to revolutionise human health by improving medical treatment through a better understanding of the molecular mechanisms of human diseases. Despite great successes of the last decade’s genome-wide association studies (GWAS), the results have been translated to genomic medicine to a limited extent. One route to get closer to improved medical treatment could be by understanding the genetics of medication use. Current medication profiles from 335,744 individuals from the UK Biobank were obtained, and a GWAS was conducted to identify common genetic variants associated with current medication use. In total, 59 independent loci were identified for medication use, and approximately 18% of the total variation was attributable to common genetic variation. The largest fraction of genetic variance for current medication use was captured by variants with low-to-medium minor allele frequency, with coding, conserved genomic regions and transcription start sites being enriched for associated variants. The average correlation (R) between medication use and the polygenic score was 0.14. The results further demonstrated that individuals with higher polygenic burden for medication use were, on average, sicker and had a higher risk for adverse drug reactions. These results provide an insight into the genetic contribution of medication use and pave the way for developments of novel multiple trait polygenic scores, which include the genetically informed medication use.
Influence Of Recreational Activities On Stress, Subjective Wellbeing And Job Satisfaction Among Lecturers In University Of Calabar

This study investigated impact of recreational activities on stress, subjective wellbeing and job satisfaction among lecturers in the University of Calabar Nigeria. Three null hypotheses guided the study. Survey research design was adopted for the study. Stratified random sampling technique was adopted to select the 150 lecturers from the faculties of education who participated in the study. The instrument for data collection was a twenty (20) item structured questionnaire which was presented for validation by two experts in Test and Measurement and one from the department of Human Kinetics and Health Education. Data were collected by physically administering copies of the instrument to the respondents. The data collected were statistically analyzed using simple linear regression and the study documented a statistically significant influence of recreational activities on stress management, subjective wellbeing and job satisfaction among lecturers in the University of Calabar. Based on the findings of the study, it is recommended among other suggestions that relevant authorities should ensure that recreational activities are deliberately introduced into lecturers’ work schedules to sustain their job satisfaction and attain educational goals and objectives.
An Experimental and Numerical Study of Long Wave Run-Up on a Plane Beach

This research is to facilitate the current understanding of long wave dynamics at coasts and during on-land propagation; experimental and numerical approaches are compared against existing analytical expressions for the long wave run-up. Leading depression sinusoidal waves are chosen to model these dynamics. The experimental study was conducted using a new pump-driven wave generator and the numerical experiments were carried out with a one-dimensional discontinuous Galerkin non-linear shallow water model. The numerical model is able to accurately reproduce the run-up elevation and velocities predicted by the theoretical expressions. Depending on the surf similarity of the generated waves and due to imperfections of the experimental wave generation, riding waves are observed in the experimental results. These artifacts can also be confirmed in the numerical study when the data from the physical experiments is assimilated. Qualitatively, scale effects associated with the experimental setting are discussed. Finally, shoreline velocities, run-up and run-down are determined and shown to largely agree with analytical predictions.
On upstream fish passage in standard box culverts: interactions between fish and turbulence

ABSTRACT The challenge to understanding the fluid mechanics of fish swimming is knowing exactly what the water is doing where the fish swims. Recent field and laboratory observations in box culvert barrel showed that fish tend to swim preferentially close to the channel sidewalls, in regions of slow velocity and often high turbulence intensity. An analogy with human swimming is developed herein. Fish minimise their energy expenditure by swimming in inter-connected low-velocity zones (LVZs) and minimising acceleration-deceleration amplitudes. In a box culvert barrel, the mechanical energy expenditure is drastically reduced in sidewall and corner flow regions, characterised by low velocities and secondary current motion. These regions were “sweet spots” used by small bodied fish to minimise their rate of work. Both bed and sidewall roughness must be scaled to the fish dimensions. More generally, the methodology brings rigorous scientific insights into why certain culvert designs, possibly equipped with baffles and apertures, are more efficient in promoting fish passage. One may foresee an evolution of the scientific approach towards using advanced physics-based theory supported by high-quality data sets. The results also raise questions on limitations of current fish swim tunnel tests, and matching swimming performance data to hydrodynamic measurements.
Synchronous Laparoscopic Radical Nephrectomy Left and Contralateral Right Hemicolectomy during the Same Endoscopic Procedure

Synchronous renal cell carcinoma in patients with colorectal carcinoma is reported in various percentages ranging from 0.03 up to 4.85% (Halak et al. (2000), Capra et al. (2003)). When surgical treatment is indicated usually two separate operations are planned for resection. In open surgery, in such cases simultaneous resection is recommended if possible. Few reports have described the resection of colorectal and renal cell carcinoma in a single laparoscopic procedure. We have shown that combining left radical nephrectomy and right hemicolectomy is technically feasible, safe and that overall operative time can be limited. In our case operative time was 210 minutes, blood loss 100 milliliters, and duration of hospital stay was 8 days. Adequate port placement, preoperative scheduling, and surgical experience are essential to achieve this goal.
Optimal Inattention to the Stock Market with Information Costs and Transactions Costs

Recurrent intervals of inattention to the stock market are optimal if consumers incur a utility cost to observe asset values. When consumers observe the value of their wealth, they decide whether to transfer funds between a transactions account from which consumption must be financed and an investment portfolio of equity and riskless bonds. Transfers of funds are subject to a transactions cost that reduces wealth and consists of two components: one is proportional to the amount of assets transferred, and the other is a fixed resource cost. Because it is costly to transfer funds, the consumer may choose not to transfer any funds on a particular observation date. In general, the optimal adjustment rule—including the size and direction of transfers, and the time of the next observation—is state-dependent. Surprisingly, unless the fixed resource cost of transferring funds is large, the consumer’s optimal behavior eventually evolves to a situation with a purely time-dependent rule with a constant interval of time between observations. This interval of time can be substantial even for tiny observation costs. When this situation is attained, the standard consumption Euler equation holds between observation dates if the consumer is sufficiently risk averse.
Distribution and expression of SLC45A2 in the skin of sheep with different coat colors.

INTRODUCTION To investigate whether the membrane-associated transporter protein SLC45A2 is differentially expressed in the skin of sheep with different coat colors and to determine its correlation with coat color establishment in sheep. MATERIAL AND METHODS The expression of SLC45A2 in sheep skin samples with different coat colors was qualitatively and quantitatively analyzed by PCR amplification, RT-PCR, immunohistochemical staining and Western blotting. RESULTS A 193-bp SLC45A2 CDS sequence was successfully amplified from sheep skin samples with diverse coat colors. RT-PCR analysis revealed that SLC45A2 mRNA was expressed in all sheep skin samples tested, with relative expression levels of 512.74 ± 121.51 in black skin, 143.38 ± 119.31 and 1.36 ± 0.09 in black dots and white dots of piebald skin, respectively, and 1.02 ± 0.23 in white skin (p < 0.01**). Positive SLC45A2 protein bands were also detected in all skin samples by Western blot analysis, with relative expression levels of 0.85 ± ± 0.17** in black skin, 0.60 ± 0.05** and 0.34 ± 0.07 in black dots and white dots of piebald skin, respectively, and 0.20 ± 0.05 in white skin (p < 0.01**). Immunohistochemical assays revealed that SLC45A2 was expressed in the hair follicle matrix, the inner and outer root sheath, and the dermal papilla in the skin tissues with different coat colors. These patterns were quantified by optical density (OD) analysis, which yielded relative expression levels of 0.23 ± 0.11 in black skin, 0.19 ± 0.09 and 0.10 ± 0.03 in black dots and white dots of piebald skin, respectively, and 0.08 ± 0.01 in white skin (p < 0.05*). CONCLUSION SLC45A2 is detectably expressed in sheep skin of all coat colors, though at significantly different levels. SLC45A2 may participate in the establishment of coat color by regulating the synthesis and trafficking of melanin.
Pseudomonas aeruginosa biofilm aggravates skin inflammatory response in BALB/c mice in a novel chronic wound model

Chronic wounds are presumed to persist in the inflammatory state, preventing healing. Emerging evidence indicates a clinical impact of bacterial biofilms in soft tissues, including Pseudomonas aeruginosa (PA) biofilms. To further investigate this, we developed a chronic PA biofilm wound infection model in C3H/HeN and BALB/c mice. The chronic wound was established by an injection of seaweed alginate‐embedded P. aeruginosa PAO1 beneath a third‐degree thermal lesion providing full thickness skin necrosis, as in human chronic wounds. Cultures revealed growth of PA, and both alginate with or without PAO1 generated a polymorphonuclear‐dominated inflammation early after infection. However, both at days 4 and 7, there were a more acute polymorphonuclear‐dominated and higher degree of inflammation in the PAO1 containing group (p < 0.05). Furthermore, PNA‐FISH and supplemented DAPI staining showed bacteria organized in clusters, resembling biofilms, and inflammation located adjacent to the PA. The chronic wound infection showed a higher number of PAO1 in the BALB/c mice at day 4 after infection as compared to C3H/HeN mice (p < 0.006). In addition, a higher concentration of interleukin‐1beta in the chronic wounds of BALB/c mice was observed at day 7 (p < 0.02), despite a similar number of bacteria in the two mouse strains. The present study succeeded in establishing a chronic PA biofilm infection in mice. The results showed an aggravating impact of local inflammation induced by PA biofilms. In conclusion, our findings indicate that improved infection control of chronic wounds reduces the inflammatory response and may improve healing.
Channel parameter estimation in mobile radio environments using the SAGE algorithm

This study investigates the application potential of the SAGE (space-alternating generalized expectation-maximization) algorithm to jointly estimate the relative delay, incidence azimuth, Doppler frequency, and complex amplitude of impinging waves in mobile radio environments. The performance, i.e., high-resolution ability, accuracy, and convergence rate of the scheme, is assessed in synthetic and real macro- and pico-cellular channels. The results indicate that the scheme overcomes the resolution limitation inherent to classical techniques like the Fourier or beam-forming methods. In particular, it is shown that waves which exhibit an arbitrarily small difference in azimuth can be easily separated as long as their delays or Doppler frequencies differ by a fraction of the intrinsic resolution of the measurement equipment. Two waves are claimed to be separated when the mean-squared estimation errors (MSEEs) of the estimates of their parameters are close to the corresponding Cramer-Rao lower bounds (CRLBs) derived in a scenario where only a single wave is impinging. The adverb easily means that the MSEEs rapidly approach the CLRBs, i.e., within less than 20 iteration cycles. Convergence of the log-likelihood sequence is achieved after approximately ten iteration cycles when the scheme is applied in real channels. In this use, the estimated dominant waves can be related to a scatterer/reflector in the propagation environment. The investigations demonstrate that the SAGE algorithm is a powerful high-resolution tool that can be successfully applied for parameter extraction from extensive channel measurement data, especially for the purpose of channel modeling.
Enantiomeric differentiation of acyclic terpenes by 13C NMR spectroscopy using a chiral lanthanide shift reagent

The 13C NMR behaviour of ten acyclic terpene alcohols was examined in the presence of a chiral lanthanide shift reagent (CLSR). For each alcohol, we measured the lanthanide‐induced shift (LIS) on the signals of the carbons and the splitting of some signals, which allowed the enantiomeric differentiation. As expected, the LIS decreased with the number of bonds between the binding function and the considered carbon. The enantiomeric splitting is observed for several signals in the spectrum of each compound. The influence of the hindrance of the binding function (primary, secondary or tertiary alcohol) and that of the stereochemistry of the double bonds is discussed. Copyright © 2004 John Wiley & Sons, Ltd.
Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

The unicellular green alga Dunaliella salina is well adapted to salt stress and contains compounds (including β-carotene and vitamins) with potential commercial value. A large transcriptome database of D. salina during the adjustment, exponential and stationary growth phases was generated using a high throughput sequencing platform. We characterized the metabolic processes in D. salina with a focus on valuable metabolites, with the aim of manipulating D. salina to achieve greater economic value in large-scale production through a bioengineering strategy. Gene expression profiles under salt stress verified using quantitative polymerase chain reaction (qPCR) implied that salt can regulate the expression of key genes. This study generated a substantial fraction of D. salina transcriptional sequences for the entire growth cycle, providing a basis for the discovery of novel genes. This first full-scale transcriptome study of D. salina establishes a foundation for further comparative genomic studies. 摘要目 的解析杜氏盐藻代谢过程, 主要关注盐胁迫下累积的代谢物 (渗透平衡产物、多胺和类胡萝卜素) 的代谢。 创新点本研究通过高通量测序产生了大量来自杜氏盐藻整个生长周期的转录组数据, 描述了杜氏盐藻在盐胁迫下累积的渗透平衡产物、 多胺和类胡萝卜素的代谢过程。 另外通过该手段也进一步分析了盐胁迫处理下, 抑制精胺合成底物的供应可能会缓解盐藻增殖对胡萝卜素含量的影响。 方 法以来自3 个不同生长时期的杜氏盐藻为材料, 进行大规模转录组测序。 在转录组功能注释的基础上, 预测了杜氏盐藻盐胁迫下累积的渗透平衡产物 (图3)、 多胺 (图4) 和类胡萝卜素 (图5) 的代谢路径。 利用相对定量聚合酶链反应 (qPCR) 技术构建了相关代谢路径中关键基因的表达谱 (图6)。 结 论通过杜氏盐藻转录组测序共获取了 39820 条单一序列。 在功能注释和聚类分析的基础上预测了杜氏盐藻盐胁迫下累积的渗透平衡产物 (甘油和脯氨酸)、 多胺以及类胡萝卜素的代谢路径。 相关代谢途径的关键酶的表达谱分析, 说明盐能够调节甘油、脯氨酸以及多胺的代谢过程。 抑制精胺合成底物的供应可能会缓解盐藻增殖对胡萝卜素含量的影响。
Downlink Multi-Antenna Heterogeneous Cellular Network With Load Balancing

We model and analyze heterogeneous cellular networks with multiple antenna BSs (multi-antenna HetNets) with K classes or tiers of base stations (BSs), which may differ in terms of transmit power, deployment density, number of transmit antennas, number of users served, transmission scheme, and path loss exponent. We show that the cell selection rules in multi-antenna HetNets may differ significantly from the single-antenna HetNets due to the possible differences in multi-antenna transmission schemes across tiers. While it is challenging to derive exact cell selection rules even for maximizing signal-to-interference-plus-noise-ratio (SINR) at the receiver, we show that adding an appropriately chosen tier-dependent cell selection bias in the received power yields a close approximation. Assuming arbitrary selection bias for each tier, simple expressions for downlink coverage and rate are derived. For coverage maximization, the required selection bias for each tier is given in closed form. Due to this connection with biasing, multi-antenna HetNets may balance load more naturally across tiers in certain regimes compared to single-antenna HetNets, where a large cell selection bias is often needed to offload traffic to small cells.