Miracle-dz/UniFAR-FTUs huggingface dataset:
Nutrient enrichment of estuarine submersed vascular plant communities. 1. Algal growth and effects on production of plants and associated communities.

Eight experimental ponds containing submersed vascular plants (predominantly Potamogeton perfoliatus and Ruppja maritirna) were subjected in duplicate to 4 levels (including controls) of fertilization from June to August 1981. Seston and phytoplankton chlorophyll a increased with fertilization, and pronounced algal blooms were evident under high dosage. Of the total seston. phytoplankton exerted the greatest influence on attenuation of photosynthetically active radiation (PAR), such that there was insufficient light for submersed vascular plant growth at the sediment surface during bloonls. An extensive epiphytic community developed on plants in all nutrient-treated ponds at densities similar to those observed in nature on senescent plants. At high nutrient treatments the accumulation of epiphytic material resulted in > 80 % attenuation of the incident radiation at the leaf surface. Biomass of submersed macrophytes decreased significantly under high and medium nutrient treatments compared to control and low treatments within 60 d following initial fertilization. Apparent production of vascular plants (based on oxygen production and I4C-bicarbonate uptake) was reduced at the higher nutrient treatments for both R. maritirna and P. perfoliatus. Most of this reduction in macrophyte photosynthesis could be explained by attenuation of PAR associated with epiphytic material. However, without PAR attenuance in the overlying water, observed levels of epiphytic growth would be insufficient to reduce light below compensation levels needed to sustain vascular plant growth. At the high fertilization rates, integrated primary production of pond communities was significantly reduced with the loss of the vascular plants, even though phytoplankton and epiphytic growth were enhanced.
Debating multiculturalism and national identity in Britain: Competing frames

Recent interpretations of policy developments across Europe have suggested a potential tension between multiculturalism and national identity. This article examines how this tension has been understood in British political debate by analysing, as a proxy, debates from the House of Lords. These debates show that four competing frames exist on the relationship between multiculturalism and national identity. These frames offer rival perspectives on the issues surrounding multiculturalism and national identity; they present different problems and solutions. Moreover, the article shows how these frames start from different interpretations of the social reality they are responding to. It concludes by questioning the pursuit of consensus on these matters.
Genotyping Techniques for Determining the Diversity of Microorganisms

Typing of microbial pathogens, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections. Strain typing also has applications in studying bacterial population dynamics. The part that molecular methods have to play in elucidating bacterial diversity is increasingly important. The shortcomings of phenotypically based typing methods (generally these methods are viewed as being too time consuming and lacking in sufficient resolution amongst related strains) have led to the development of many DNA – based techniques. A suitable typing method must have high discrimination power combined with good to moderate interand intra-laboratory reproducibility. In addition, it should be easy to set up, to use and to interpret, and inexpensive (Olive & Bean, 1999). In this chapter, we review the current bacterial genotyping methods and classify them into six main categories: (1 and 2) DNA banding pattern-based methods, which classify bacteria according to the size of fragments generated respectively by enzymatic digestion of genomic/plasmid DNA, and PCR amplification, (3) DNA hybridization–based methods using nucleotidic probes, (4) DNA sequencing-based methods, which study the polymorphism of DNA sequences, (5) differentiation of isolates on the basis of presence or absence of particular genes and (6) high resolution melting analysis–real–time monitoring of melting process of PCR amplified polymorphic DNA fragment. We described and compared the applications of genotyping methods to the study of bacterial strain diversity. We also discussed the selection of appropriate genotyping methods and the challenges of bacterial strain typing and described the current trends of genotyping methods.
On the relationship between fronts of the Antarctic Circumpolar Current and surface chlorophyll concentrations in the Southern Ocean

[1] We reexamine the relationship between circulation, bathymetry, and surface chlorophyll in the Southern Ocean, using new high-resolution maps of the frontal structure of the Antarctic Circumpolar Current (ACC) derived from satellite altimetry. The maps reveal that the ACC consists of multiple filaments or jets. By averaging surface chlorophyll measurements along streamlines, we show that the fronts define the limits of zones with similar concentrations and seasonality of surface chlorophyll. The overall pattern of surface chlorophyll is consistent with strongest upwelling of nutrient-rich deep water south of the Polar Front (PF). However, the distribution of chlorophyll in the Southern Ocean is concentrated in a number of persistent blooms, observed downstream of islands and bathymetric features. In contrast to previous studies, we find little evidence that the fronts of the ACC are associated with enhanced productivity, at least where the fronts are distant from topography. Rather, we find that most regions of elevated chlorophyll in the open Southern Ocean can be explained by upwelling of nutrients (both macronutrients and micronutrients) where the ACC interacts with topography, followed by downstream advection. The upwelling is shown to be the consequence of the bottom pressure torque established by the large-scale flow, rather than being due to small-scale instabilities of the jets. The interaction of the flow with the topography therefore establishes both the large-scale dynamical balance of the ACC and determines the productivity of the open Southern Ocean.
Prognostic relevance of viable circulating tumor cells detected by EPISPOT in metastatic breast cancer patients.

BACKGROUND Detection of circulating tumor cells (CTC) in breast cancer patients is currently performed in many clinical trials, using different technologies, in particular the EpCAM-dependent CellSearch® system. The purpose of this study was to investigate the incidence and prognostic relevance of viable CTC in a large cohort of metastatic breast cancer (MBC) patients. METHODS A total of 254 MBC patients were enrolled in a prospective multicenter study at first diagnosis of metastatic disease or disease progression (before the start of a new treatment regimen). After EpCAM-independent enrichment, viable CTC releasing cytokeratin-19 as an epithelial cell marker were detected in the peripheral blood by an EPISPOT assay, and the Food and Drug Administration cleared CellSearch was used as the reference method. RESULTS Using the EPISPOT assay, CTC were detected in 59% of MBC patients. The overall survival (OS) was linked with the CTC status measured by EPISPOT (P = 0.0191), which allowed stratification of MBC patients in low- and high-risk groups. This stratification could be improved by addition of the CTC status assessed by the CellSearch system. In multivariate Cox proportional-hazards regression analysis, the 3 methods used to determine the level of CTC (EPISPOT, CellSearch, and combination of EPISPOT/CellSearch) were compared by the Bayesian information criterion method. Interestingly, the combination of the EPISPOT and CellSearch assays was the strongest predictor of OS (hazard ratio, 22.6; 95% CI, 2.8-184.08). CONCLUSIONS This is the first study in which CTC detection using the EPISPOT assay was evaluated on a large cohort of MBC patients, showing prognostic relevance of the presence of viable CTC.
Decompression, Enucleation, and Implant Placement in the Management of a Large Dentigerous Cyst

The dentigerous cyst is a developmental odontogenic cyst, accounting for 10% of all cysts of the jaws. The treatment options are decompression, marsupialization, or enucleation regarding the features of the lesions. In this article, a case of a 17-year-old adolescent girl having a large mandibular dentigerous cyst associated with an unerupted third molar is presented. The treatment is composed of decompression for 6 months and enucleation. Decompression and enucleation are a predictable choice in the treatment of large cysts. Radiologic evaluation is mandatory to decide for the appropriate time for the removal of the decompression stents, the enucleation time, and also for the evaluation of the adequate new bone formation for implant placement. The present case emphasizes the success of a conservative approach in the management of a large dentigerous cyst in a young patient and also the maintenance of the new bone formation without using any graft materials for the placement of dental implants.
An improved limit on the axion–photon coupling from the CAST experiment

We have searched for solar axions or similar particles that couple to two photons by using the CERN Axion Solar Telescope (CAST) set-up with improved conditions in all detectors. From the absence of excess x-rays when the magnet was pointing to the Sun, we set an upper limit on the axion–photon coupling of gaγ<8.8 × 10−11 GeV−1 at 95% CL for . This result is the best experimental limit over a broad range of axion masses and for also supersedes the previous limit derived from energy-loss arguments on globular cluster stars.
Neutrophils, apoptosis and phagocytic clearance: an innate sequence of cellular responses regulating intramacrophagic parasite infections

In complex organisms, apoptosis is a constitutive cell death process that is involved in physiological regulation of cell numbers and that can also be induced in the course of inflammatory and immune responses. Neutrophils are among the first cells recruited during inflammation. Neutrophils constitutively die by apoptosis at inflamed sites, and are ingested by macrophages. Recent studies investigated how phagocytic clearance of senescent neutrophils influences the survival of intracellular protozoan parasites that have been phagocytosed by, or have invaded phagocytes. The results indicate that neutrophil clearance plays an unexpected role in regulation of intramacrophagic protozoan parasite infection.
Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi

Significance Malaria continues to impose enormous health and economic burdens on the developing world. Novel technologies proposed to reduce the impact of the disease include the introgression of parasite-resistance genes into mosquito populations, thereby modifying the ability of the vector to transmit the pathogens. Such genes have been developed for the human malaria parasite Plasmodium falciparum. Here we provide evidence for a highly efficient gene-drive system that can spread these antimalarial genes into a target vector population. This system exploits the nuclease activity and target-site specificity of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, which, when restricted to the germ line, copies a genetic element from one chromosome to its homolog with ≥98% efficiency while maintaining the transcriptional activity of the genes being introgressed. Genetic engineering technologies can be used both to create transgenic mosquitoes carrying antipathogen effector genes targeting human malaria parasites and to generate gene-drive systems capable of introgressing the genes throughout wild vector populations. We developed a highly effective autonomous Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (Cas9)-mediated gene-drive system in the Asian malaria vector Anopheles stephensi, adapted from the mutagenic chain reaction (MCR). This specific system results in progeny of males and females derived from transgenic males exhibiting a high frequency of germ-line gene conversion consistent with homology-directed repair (HDR). This system copies an ∼17-kb construct from its site of insertion to its homologous chromosome in a faithful, site-specific manner. Dual anti-Plasmodium falciparum effector genes, a marker gene, and the autonomous gene-drive components are introgressed into ∼99.5% of the progeny following outcrosses of transgenic lines to wild-type mosquitoes. The effector genes remain transcriptionally inducible upon blood feeding. In contrast to the efficient conversion in individuals expressing Cas9 only in the germ line, males and females derived from transgenic females, which are expected to have drive component molecules in the egg, produce progeny with a high frequency of mutations in the targeted genome sequence, resulting in near-Mendelian inheritance ratios of the transgene. Such mutant alleles result presumably from nonhomologous end-joining (NHEJ) events before the segregation of somatic and germ-line lineages early in development. These data support the design of this system to be active strictly within the germ line. Strains based on this technology could sustain control and elimination as part of the malaria eradication agenda.
AN ANALYSIS OF DRIVERS AFFECTING THE IMPLEMENTATION OF GREEN SUPPLY CHAIN MANAGEMENT FOR THE INDIAN MANUFACTURING INDUSTRIES

The supply chain consists of those activities assoc iated with manufacturing from raw material acquisit ion to final product delivery. Because of the recently changed environmental requi rements that affect manufacturing operations and tr ansportation systems, growing attention is given to the development of en vironment management strategies for supply chains. A green supply chain aims at confining the wastes within the industrial system s o as to conserve energy and prevent the dissipation of harmful materials into the environment. Frequently researches in study on driv ers of Green Supply Chain Management are criticized for its narrow perspectives and lack of industrial relevance. So, it is a real need to find improved processes for adoption and im plementation of Green practices Green image in Manufacturing Industries. In the cur rent competitive scenario, the driver of Green Supp ly Chain Management assumes a significant importance and call for serio us research attention. The aim of this paper is to examining the Green practices in the selected Indian Manufacturing Companies. The ma in objectives of this paper are to gain critical and crucial drivers for implementation of Green Supply Chain Management in Indian Manufacturing Industries in various sectors likewise, two wheeler, four wheeler, and General Manufacturing sector wise comp anies. This paper has attempted to fill the gaps in the contemporary research in the study on drivers of Green Supply Chain Manageme nt in selected Indian corporate sectors. It is obse rved that Driver, DF10 i.e. ‘Employees Motivation, health & Safety’ have more i
The Avian Transcriptome Response to Malaria Infection

Malaria parasites are highly virulent pathogens which infect a wide range of vertebrates. Despite their importance, the way different hosts control and suppress malaria infections remains poorly understood. With recent developments in next-generation sequencing techniques, however, it is now possible to quantify the response of the entire transcriptome to infections. We experimentally infected Eurasian siskins (Carduelis spinus) with avian malaria parasites (Plasmodium ashfordi), and used high-throughput RNA-sequencing to measure the avian transcriptome in blood collected before infection (day 0), during peak parasitemia (day 21 postinfection), and when parasitemia was decreasing (day 31). We found considerable differences in the transcriptomes of infected and uninfected individuals, with a large number of genes differentially expressed during both peak and decreasing parasitemia stages. These genes were overrepresented among functions involved in the immune system, stress response, cell death regulation, metabolism, and telomerase activity. Comparative analyses of the differentially expressed genes in our study to those found in other hosts of malaria (human and mouse) revealed a set of genes that are potentially involved in highly conserved evolutionary responses to malaria infection. By using RNA-sequencing we gained a more complete view of the host response, and were able to pinpoint not only well-documented host genes but also unannotated genes with clear significance during infection, such as microRNAs. This study shows how the avian blood transcriptome shifts in response to malaria infection, and we believe that it will facilitate further research into the diversity of molecular mechanisms that hosts utilize to fight malaria infections.
“The Survey of the Ghetto” in the Time of Anti-Semitism

This paper aims to recall one of the first pieces of anthropological fieldwork carried out in Europe. It was supervised by Bronisław Malinowski and carried out by Feliks Gross (1906–2006). He had been a young Cracovian lawyer of Jewish descent and socialist activist interested in sociology and anthropology, who was preparing for an academic career. This turned out to be impossible because of the growing wave of anti-Semitism in Europe. Gross became a student and collaborator of his Cracovian compatriot, then a London professor, Bronisław Malinowski, who advised him to make a “survey of the ghetto” in their hometown. The research progressed promisingly, but was halted by the outbreak of the Second World War. Gross managed to escape to Vilna, where he tried to continue his research. He finally landed safely in the United States, where he met his mentor and encouraged him to engage in political activity. The Cracovian project is described using the correspondence between Malinowski and Gross, as well as other archival material. It is shown against the political and academic background, together with the important theoretical and methodological frameworks of the time. Thus, the unsuccessful project becomes a very informative case of the working of the academic “field,” to use Pierre Bourdieu’s term. There are also other examples of European research supervised by Malinowski, who, therefore, deserves the title of a predecessor of the “anthropology at home. ”
The Ratio of Constitutive Androstane Receptor to Pregnane X Receptor Determines the Activity of Guggulsterone against the Cyp2b10 Promoter

Guggulsterone is the active ingredient in gugulipid, an organic extract of the Commiphora mukul plant. Gugulipid has been used for nearly 3000 years in Ayurvedic medicine, mainly as a treatment for arthritis. Herbal practitioners currently use gugulipid therapy in conditions as diverse as rheumatism, coronary artery disease, arthritis, hyperlipidemia, acne, and obesity. The active ingredient in gugulipid is guggulsterone, a plant sterol compound recently identified as a pregnane X receptor (PXR; NR1I2) ligand. We show herein that guggulsterone treatment represses the expression of cytochrome P450 2b10 (Cyp2b10) gene expression by inhibiting constitutive androstane receptor (CAR; NR1I3) activity in hepatocytes lacking functional PXR (PXR-knockout). We also show that PXR-CAR cross-talk determines the net activity of guggulsterone treatment toward Cyp2b10 gene expression. Using mammalian two-hybrid assays, we show that treatment with guggulsterone differentially affects protein cofactor recruitment to these two nuclear receptors. These data identify guggulsterone as an inverse agonist of the nuclear receptor CAR. When viewed together with the data showing that PXR and CAR expression is highly variable in different ethnic populations and that CAR expression is under the control of a circadian rhythm, our data provide important insight into the molecular mechanism of interindividual variability of drug metabolism. These data, together with the recent resolution of the crystal structures of PXR and CAR, will likely aid in the rational design of more specific CAR inverse agonists that are currently viewed as potential antiobesity drugs.
Are transrectal ultrasonically guided biopsies required for the accurate diagnosis of carcinoma of the prostate? Can digitally guided systematic biopsies offer an acceptable alternative?

OBJECTIVE To compare the diagnostic yield of ultrasonically guided and digitally guided systematic biopsies of the prostate. PATIENTS AND METHODS Fifty two patients with suspected but unproven carcinoma of the prostate underwent simultaneous transrectal ultrasonography (TRUS)-guided and digitally guided systematic biopsies of the prostate. A mean of 12 cores was obtained from each patient. On average more cores were obtained with TRUS guidance (mean 6.7) than with digital guidance (mean 5.3). RESULTS Cancer was detected in 19 patients and the diagnosis was made by both techniques in 16. Two patients had tumour detected only with TRUS guidance and one only with digital guidance. No complications occurred. CONCLUSION This study indicates that there is no significant difference between TRUS guidance and digital guidance to direct systematic biopsies of the prostate. The use of routine TRUS in screening programmes is now in doubt.
Risk of Reverse Seroconversion of Hepatitis B Virus Surface Antigen in Rituximab-Treated Non-Hodgkin Lymphoma Patients

AbstractRituximab causes hepatitis B virus (HBV) reactivation in HBV surface antigen (HBsAg)-seronegative patients with CD20-positive B-cell non-Hodgkin lymphoma (CD20+ NHL), especially for those seropositive to the antibody of core antigen (anti-HBc). Clinical hepatitis usually develops after reverse seroconversion of HBsAg (HBV-RS), indicated by the reappearance of HBsAg in serum. Because of the relatively high prevalence of anti-HBc seropositivity in unvaccinated HBsAg-seronegative adults in an HBV hyperendemic area, we aimed to investigate additional factors influencing the development of rituximab-associated HBV-RS.Between January 2000 and December 2010, unvaccinated HBsAg-seronegative adults with CD20+ NHL who had received rituximab-containing therapy but not anti-HBV agents were enrolled. Patients with and without HBV-RS were compared in terms of clinical factors and treatments including the number of cycles of rituximab therapy, and transplantation. Competing risk regression was used to identify the factors associated with HBV-RS.For the 482 patients enrolled, the serological status of anti-HBc was available in 75.9%, with a seropositivity rate of 86.6%. At the last follow-up, a total of 33 (6.85%) patients had HBV-RS, with 95.8% anti-HBc seropositive, 78.9% anti-HBs seropositive, and none anti-HCV seropositive. HBV-RS patients have received more cycles (≥6) and prolonged durations of rituximab therapy, and hematopoietic stem cell transplantation. The overall survival was not different between patients with and those without HBV-RS. At the time of HBV-RS, a total of 25 (78.1%) patients had hepatitis flare, especially when HBV-RS appeared during/after induction therapy (100%, 10 of 10). Three (9.1%) patients had fulminant hepatitis, resulting in death in 1 (3%) patient. A higher rituximab cycle intensity was associated with a higher rate of hepatitis flare at the time of HBV-RS. When death in the absence of HBV-RS was considered as the competing risk, the univariate and multivariate regression analyses showed that several factors were independently associated with the development of HBV-RS, including anti-HCV seronegativity, histological subtype of posttransplant lymphoproliferative disorders, ≥6 cycles of rituximab therapy, and succeeding hematopoietic stem cell transplantation.The findings of our study identify additional factors influencing the development of rituximab-associated HBV-RS in HBsAg-seronegative adults with CD20+ NHL.