Miracle-dz/UniFAR-FTUs huggingface dataset:
Translocation of Sugars in the Concord Grape.

translocatability of the various sugars varies significantly with species, and perhaps with environmental conditions as well. The analysis of this problem is admittedly complicated by the difficulty of distinguishing between the molecules in the phloem actually in transit from the leaves or other supply centers and molecules of the same species originating in metabolic reactions in this tissue, or captured in metabolic pools of the respective sugars in the non-conducting cells of the phloem and closely adjacent tissues. However, the techniques of radiochemistry and chromatography now permit a more definitive analysis of this problem, and a re-examination, therefore, of certain aspects of sugar translocation has been undertaken. The grape was chosen for the initial studies in this series because of the ease with which the bark and wood may be separated for analytical purposes, and because of the extensive information available on the anatomy and cytology of its phloem (6).
Spondyloepimetaphyseal dysplasia Pakistani type: Expansion of the phenotype

Spondyloepimetaphyseal dysplasia (SEMD), Pakistani type, is a skeletal dysplasia characterized by platyspondyly, delayed epiphyseal ossification, mild metaphyseal abnormalities, short stature, and short and bowed legs, and is caused by mutations in PAPSS2. In a single Turkish patient also hyperandrogenism was reported. We describe five patients from a Turkish family with SEMD Pakistani type with homozygosity for a nonsense mutation (p.R329X) leading to a stop codon in PAPSS2. Plasma levels of dehydroepiandrosterone (DHEA) and androstenedione were normal, but DHEA sulfate levels were low in four of the patients. Two patients and a mother had history of pubertal hyperandrogenism. Testosterone level was mildly elevated in one of the female patients, and insulin resistance was not detected in any of the patients. The patients also had precocious costal calcification, small iliac bones, short femoral necks, coxa vara, short halluces and fused vertebral bodies, none of which has been reported previously in this entity. (c) 2013 Wiley Periodicals, Inc.
Low cost measurement system for broadcast power amplifiers

For the fast prototyping, production, and delivery of commercial radio frequency products/systems, accurate and reliable test systems are necessary. These systems are usually product customized and thus expensive solutions, capable of performing all necessary measurements for a specific product. The purpose of this paper is to present a flexible cost‐effective test solution, capable of realizing all necessary measurement on a high output power amplifier system greater than 500 W, and to generate essential reports. These reports allow the system operator to (i) perform an analysis and a statistical comparison among various products, assuring that the quality is the same for all products; and (ii) retain products that present a performance poorer than other similar products, without a rigorous technical analysis, thus increasing efficiency in factory assembly line. Copyright © 2013 John Wiley & Sons, Ltd.
Interleukin-6: Possible biological roles during exercise

Abstract Interleukin-6 (IL-6) is a multifunctional cytokine that exerts its modulatory effects on cells that express membrane bound IL-6 receptors; however, IL-6 in a complex with soluble IL-6R can bind to any cell that express glycoprotein 130 (gp130). Thus, all cell types may respond to the pro- as well as anti-inflammatory properties of IL-6. Since the first report of acute exercise-induced increase in plasma IL-6 in the early 1990s, scientists have tried to elucidate the factors that influence the magnitude of change of plasma IL-6, as well as the possible biological roles of this cytokine. Evidence suggests that exercise intensity and duration as well as the form of contraction (e.g. eccentric or concentric) and muscle damage all influence IL-6 response to acute exercise. However, data on training status and performance on plasma IL-6 concentration changes during exercise are more inconclusive, as discussed in this review. In the last decade, most of the studies have focused on IL-6 as an ‘energy sensor’ possibly secreted by skeletal muscle that activates glycogenolysis in the liver and lipolysis in fat tissue in order to provide muscle with the growing energy demands during exercise.
Relationships between muscular torque and gait speed in chronic hemiparetic subjects

Purpose. To identify which paretic and non-paretic muscular groups of the lower limbs were the best predictors of gait speed in chronic hemiparetic subjects. Method. Twelve hemiparetic subjects with ages ranging from 65 to 75 years (70.67 ± 3.31 years) were included in this cross-sectional study. All participants had time since onset of stroke of at least six months and the ability to walk independently. Main outcome measures included self-selected gait speed and maximum isometric torques of both the paretic and non-paretic lower limbs. Pearson's correlation coefficients and stepwise regression analysis were employed to investigate the level of associations between the outcome variables. Results. Mean gait speed was 0.65 ± 0.33 ms−1. There were significant differences (p < 0.01) in torques values between paretic and non-paretic lower limbs for all assessed muscular groups. Stepwise regression analysis revealed that the most important independent variable for predicting gait speed was the isometric torque of paretic knee flexors (). No significant associations were found between any torque values of the non-paretic side and gait speed. Conclusions. The paretic knee flexors were shown to play an important role in predicting gait speed after stroke, but no influences were found for the muscular groups of the non-paretic side. Therefore, findings of the present study suggest that paretic knee flexors torque should be addressed in rehabilitation programmes aiming to improve gait speed after stroke.
Robin sequence: what the multidisciplinary approach can do

Robin sequence (RS) is a commonly encountered triad of micrognathia, glossoptosis, and airway obstruction, with or without a cleft palate. The management of airway obstruction is of paramount importance, and multiple reviews and retrospective series outline the diagnosis and treatment of RS. This article focuses on the multidisciplinary nature of RS and the specialists’ contributions and thought processes regarding the management of the RS child from birth to skeletal maturity. This review demonstrates that the care of these children extends far beyond the acute airway obstruction and that thorough monitoring and appropriate intervention are required to help them achieve optimal outcomes.
A clinicopathological study of nm23-H1 expression in classical Hodgkin's lymphoma.

BACKGROUND We carried out immunohistochemistry to examine the expression of nm23-H1 in Hodgkin and Reed-Sternberg cells in patients with classical Hodgkin's lymphoma (CHL). PATIENTS AND METHODS We evaluated 128 patients with CHL [87 patients with nodular sclerosis (NS) and 41 patients with mixed cellularity (MC)] for CD15, CD20, Ki-67, EBER, TIA-1, and nm23-H1 by immunohistochemistry. RESULTS CD15 was expressed in 79%, CD20 in 11%, Ki-67 in 93%, EBER in 34%, TIA-1 in 11%, and nm23-H1 in 60% of the CHL patients. NS patients showed a significantly higher rate of nm23-H1 expression than MC patients (P < 0.001). The serum nm23-H1 level was significantly higher in patients with positive nm23 expression. Univariate analysis showed that stage IV, poor performance status, low hemoglobin level, low serum albumin level, age of 45 years or older, TIA-1-positive status, and nm23-H1-positive status were associated with significantly shorter progression-free survival. Multivariate analysis with these factors showed TIA-1 and cytoplasmic nm23-H1 expression to be significant and independent prognostic factors. CONCLUSIONS Our results indicate that nm23-H1 expression is a prognostic factor for CHL and that it is as important as serum nm23-H1, both of which are useful for planning the treatment strategy.
Microstructural properties and mechanical behavior of magnesium/hydroxyapatite biocomposite under static and high cycle fatigue loading

In this study, magnesium/hydroxyapatite biocomposites having 2.5 and 5 wt% of hydroxyapatite have been fabricated using stir casting followed by hot extrusion method. Both microstructural and mechanical behaviors of these composites have been studied, which contain particle distribution, grain size, microhardness, tension, and compression tests. Then, high cycle fatigue tests were performed using a rotating-bending testing machine (under stress ratio of R = −1). The results indicate an acceptable particle distribution and grain refinement in the composites. Also, the microhardness of the composites has been increased in comparison with the pure extruded sample. The yield stress has been enhanced in both tension and compression tests by increasing the amount of reinforcement, while the maximum strain reduced. Moreover, the fabricated biocomposites revealed better overall high cycle fatigue life in comparison to pure Mg, and infinite life (>107 cycles) could be achieved in the composites. Scanning electron microscope images of the fracture surfaces showed that the agglomeration of hydroxyapatite particles is one of the most important criteria for crack initiation in the composites.
Quinolone and Cephalosporin Resistance in Enteric Fever

Enteric fever is a major public health problem in developing countries. Ciprofloxacin resistance has now become a norm in the Indian subcontinent. Novel molecular substitutions may become frequent in future owing to selective pressures exerted by the irrational use of ciprofloxacin in human and veterinary therapeutics, in a population endemic with nalidixic acid-resistant strains. The therapeutics of ciprofloxacin-resistant enteric fever narrows down to third- and fourth-generation cephalosporins, azithromycin, tigecycline and penems. The first-line antimicrobials ampicillin, chloramphenicol and co-trimoxazole need to be rolled back. Antimicrobial surveillance coupled with molecular analysis of fluoroquinolone resistance is warranted for reconfirming novel and established molecular patterns for therapeutic reappraisal and for novel-drug targets. This review explores the antimicrobial resistance and its molecular mechanisms, as well as novel drugs in the therapy of enteric fever.
Design and stability analysis of a lane following controller

This paper considers the design and stability analysis of a steering controller. The objective of the controller is to steer a ground vehicle along a reference line located in the middle of the lane to be followed. We define an arbitrary look-ahead point located on the local longitudinal axis of the vehicle. The distance between the look-ahead point and the reference line is called the look-ahead offset. During perfect lane tracking, the ratio of the steer angle to the look-ahead offset is independent of the curve radius under reasonable approximations. That ratio is computed in terms of the vehicle speed and various vehicle parameters. Then, a constant controller is designed to achieve that ratio at steady state. The controller is a continuous function of the vehicle speed. The only information processed by the controller is the look-ahead offset, which can be measured using a radar-based or a vision-based sensor. Using Routh-Hurwitz analysis, we analytically prove that the closed-loop system is stable. Given any range of longitudinal speeds, there exists a sufficiently large look-ahead distance ensuring the closed-loop stability for all speeds in that speed range. For a particular set of parameter variations, it is also shown that choosing the look-ahead distance large enough guarantees the robustness of closed-loop stability, Various simulation results demonstrating the performance of the controller are included.
Analysis of process parameters effects on underwater friction stir welding of aluminum alloy 6082-T6

In friction stir welding of heat treatable aluminum alloys, the thermal cycles developed during the joining process result in softening of the joints which adversely affect their mechanical properties. Underwater friction stir welding can be a process of choice to overcome this problem due to low peak temperature and short dwell time involved during the process. Consequently, this article presents a study pertaining to the underwater friction stir welding of aluminum alloy 6082-T6 with an aim to develop a mathematical model to optimize the underwater friction stir welding process parameters for obtaining maximum tensile strength. The results of the study reveal that the tool shoulder diameter (d), tool rotational speed (ω), welding speed (v), and second-order term of rotational speed, that is, ω2, significantly affect the tensile strength of the joint. The maximum tensile strength of 241 MPa which is indeed 79% of the base metal strength and 10.7% higher than that of conventional (air) friction stir welding joint was achieved at an optimal setting of the underwater friction stir welding parameters, that is, tool rotational speed of 900 r/min, the welding speed of 80 mm/min, and a tool shoulder of 17 mm. The article also presents the results of temperature variation, the macrostructural and microstructural investigations, microhardness, and fractography of the joint obtained at the optimal setting for underwater friction stir welded (UFSWed) joint.
Usefulness of Microcatheters Inserted Overnight for Additional Injection of Sclerosant after Initial Balloon-Occluded Retrograde Transvenous Obliteration of Gastric Varices

In patients with large gastric varices, dose limitation of the sclerosant can cause difficulties in achieving complete thrombosis of varices during a single balloon-occluded retrograde transvenous obliteration (BRTO) procedure. For patients with incomplete variceal thrombosis after the first BRTO, additional sclerosant must be injected in a second BRTO. We report a successful case of BRTO for large gastric varices in whom additional sclerosant was injected through a microcatheter that remained inserted overnight. To achieve complete variceal thrombosis in a patient with incomplete thrombosis of large gastric varices after a first BRTO, a retained microcatheter can be used to inject additional sclerosant in a second BRTO the next day.
Asphalt pavement rutting simulated using granular micromechanics-based rate-dependent damage-plasticity model

Abstract Repeated application of vehicle loads on the asphalt pavement creates permanent deformations and surface cracks. Simulation of this pavement distress has posed severe challenges due to the granular nature of the pavement materials. In this paper, the granular micromechanics approach is used in a thermo-mechanical framework to obtain the constitutive relationships for the non-linear rate-dependent granular materials with damage and plasticity. The developed constitutive relationships were validated and verified with experimental data and implemented into a non-standard finite-element (FE) framework to simulate asphalt pavement performance under the repeated traffic load. The FE model was subjected to different repeated load levels to demonstrate response nonlinearity. The applied load was subsequently removed and pavement was allowed to recover to a stress free condition to obtain the permanent deformation. The simulations results demonstrated that 30% increase in the applied load causes approximately 127% increase in the rutting depth at the centre of the applied load. Critically horizontal tensile plastic strains were observed near tire edge, indicating top-down cracking of pavement under the applied load. The presented approach provides an alternative for simulating asphalt pavement rutting by incorporating the effects of micro-scale mechanisms on the macro-scale rutting response in an FE framework. The advantage of the granular micromechanical approach is that, the damage, plastic potential and flow rule are defined using simple 1d functions at micro-scale, in contrast to tensorial formulation of complex plastic potentials, damage functions and rules for their evolution in conventional approaches.
Tightening the Bounds on Feasible Preemption Points

Caches have become invaluable for higher-end architectures to hide, in part, the increasing gap between processor speed and memory access times. While the effect of caches on timing predictability of single real-time tasks has been the focus of much research, bounding the overhead of cache warm-ups after preemptions remains a challenging problem, particularly for data caches. This paper makes multiple contributions. 1) We bound the penalty of cache interference for real-time tasks by providing accurate predictions of data cache behavior across preemptions, including instruction cache and pipeline effects. We show that, when considering cache preemption, the critical instant does not occur upon simultaneous release of all tasks. 2) We develop analysis methods to calculate upper bounds on the number of possible preemption points for each job of a task. To make these bounds tight, we consider the entire range between the best-case and worst-case execution times (BCET and WCET) of higher priority jobs. The effects of cache interference are integrated into the WCET calculations by using a feedback mechanism to interact with a static timing analyzer. Significant improvements in tightening bounds of up to an order of magnitude over two prior methods and up to half a magnitude over a third prior method are obtained by experiments for (a) the number of preemptions, (b) the WCET and (c) the response time of a task. Overall, this work contributes by calculating the worst-case preemption delay under consideration of data caches
Crohn's disease genotypes of patients in remission vs relapses after infliximab discontinuation.

AIM To investigate genetic differences between Crohn's disease (CD) patients with a sustained remission vs relapsers after discontinuing infliximab while in corticosteroid-free remission. METHODS Forty-eight CD patients received infliximab and were in full corticosteroid-free clinical remission but then discontinued infliximab for reasons other than a loss of response, were identified by review of an electronic database and charts. Infliximab-associated remission was defined as corticosteroid-free plus normalization of clinical disease activity [CD activity index (CDAI) < 150] during follow-up visits based on physician global assessments. A CD relapse (loss of infliximab-induced remission) was clinically defined as a physician visit for symptoms of disease activity (CDAI > 220) and a therapeutic intervention with CD medication(s), or a hospitalization with complications related to active CD. Genetic analyses were performed on samples from 14 patients (n = 6 who had a sustained long term remission after stopping infliximab, n = 8 who rapidly relapsed after stopping infliximab). Nucleotide-binding oligomerization domain 2 (NOD2)/caspase activation recruitment domain 15 (CARD15) polymorphisms (R702W, G908R and L1007fs) and the inflammatory bowel disease 5 (IBD5) polymorphisms (IGR2060a1 and IGR3081a1) were analyzed in each group. RESULTS Five single nucleotide polymorphisms of IBD5 and NOD2/CARD15 genes were successfully analyzed for all 14 subjects. There was no significant increase in frequency of the NOD2/CARD15 polymorphisms (R702W, G908R and L1007fs) and the IBD5 polymorphisms (IGR2060a1 and IGR3081a1) in either group of patients; those whose disease relapsed rapidly or those who remained in sustained long term remission following the discontinuation of infliximab. Nearly a third of patients in full clinical remission who stopped infliximab for reasons other than loss of response remained in sustained clinical remission, while two-thirds relapsed rapidly. There was a marked difference in the duration of clinical remission following discontinuance of infliximab between the two groups. The patients who lost remission did so after 1.0 years ± 0.6 years, while those still in remission were at the time of this study, 8.1 years ± 2.6 years post-discontinuation of infliximab, P < 0.001. The 8 patients who had lost remission after discontinuing infliximab had a mean number of 5 infusions (range 3-7), with a mean treatment time of 7.2 mo (range 1.5 mo-15 mo). The mean duration of time from the last infusion of infliximab to the time of loss of remission was 382 d (range 20 d-701 d). The 6 patients who remained in remission after discontinuing infliximab had a mean number of 6 infusions (range 3-12), with a mean treatment duration of 12 mo (range 3.6 mo-32 mo) (P = 0.45 relative to those who lost remission). CONCLUSION There are no IBD5 or NOD2/CARD15 mutations that predict which patients might have sustained remission and which will relapse rapidly after stopping infliximab.
Optimisation of growth hormone production by muscle cells using plasmid DNA.

The production of peptide hormones by skeletal muscle tissue is a promising area of gene therapy. Skeletal muscle myogenesis can be induced in vitro, resulting in the fusion of mononucleate myoblasts to form multinucleate myotubes, and delivery vectors are first tested in vitro. C2C12 myoblasts transfected with pcDNA3-GH, which used the human cytomegalovirus (CMV) promoter, secreted immunoreactive GH with comparable biological activity to pituitary GH. Mouse myeloid leukaemia cells, which express the mouse GH receptor were used for the bioassay, and activation of these cells by GH was measured by a colorimetric microculture tetrazolium assay. Cells were incubated with a tetrazolium salt (MTS) and an intermediate electron acceptor (phenazine methosulphate, PMS), and formazan production was measured as optical density (O.D.) at 490 nm. The efficiencies of several plasmid expression vectors were compared in differentiated and non-differentiated muscle cells, as a function of bioactive GH secreted by the transfected cells. Ten-day differentiated C2C12 myotubes transfected with pcDNA3E-GH, which used the CMV promoter and a rat myosin light chain enhancer element, secreted significantly more biologically active GH than myotubes transfected with pcDNA3-GH (0.82 O.D. units+/-0.06 vs 0.57+/-0.05 respectively, P<0.001). This was consistent with reduced CMV promoter activity in myotubes. Myoblasts transfected with pcDNA3-GH secreted more bioactive GH than 10-day transfected myotubes (1.1+/-0. 1 vs 0.77+/-0.07 respectively). However, the responses were indistinguishable (both 1.0+/-0.09) if both the myotubes and myoblasts had been transfected with pcDNA3E-GH. Substitution of the vector pMHLC-GH, which used a muscle-specific truncated rabbit myosin heavy chain promoter, and the myosin enhancer resulted in a marked decrease in the responses to the conditioned medium from fused myotubes compared with the vectors pcDNA3-GH and pcDNA3E-GH (0. 24+/-0.02 vs 0.57+/-0.05 vs 0.82+/-0.06 respectively). We concluded that the combination of CMV promoter and myosin light chain enhancer in pcDNA3E-GH had the greatest expression efficiency of the several plasmid vectors which we investigated.