Miracle-dz/UniFAR-FTUs huggingface dataset:
Detection of Candida species in pregnant Chinese women with a molecular beacon method

Purpose. Candida pathogens are commonly found in women and can cause vulvovaginal candidiasis (VVC), whose infection rate is further increased during pregnancy. We aimed to study the Candida prevalence and strain distribution in pregnant Chinese women with a molecular beacon assay. Methodology. From March 2016 to February 2017, a total of 993 pregnant women attending routine antenatal visits at the Beijing Obstetrics and Gynecology Hospital were enrolled. For Candida detection and identification, a unique molecular beacon assay was presented and compared with a traditional phenotypic method. Antifungal susceptibility was tested with the following agents: 5‐flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole. Results. The prevalence of Candida was found to be 21.8 % when using the molecular method and 15.0 % when using the phenotypic method. The distribution of the Candida spp. was listed in order of decreasing prevalence: Candida albicans (79.8 %), Candida glabrata (13.5 %), Candida parapsilosis (3.7 %), Candida krusei (2.2 %) and Candida tropicalis (1.1 %). We found that 90.7 % of the Candida detection results were consistent between the molecular and the phenotypic methods. In the cases where the sequencing analyses for the Candida isolates resulted in inconsistent identification, the molecular method showed higher sensitivity than the phenotypic method (96.0 vs 64.6 %). C. albicans, C. glabrata and C. parapsilosis were essentially susceptible to all five antifungal agents tested, whereas C. tropicalis and C. krusei were susceptible to voriconazole and amphotericin B. Conclusion. By exhibiting good sensitivity and specificity, the molecular assay may offer a fast and accurate Candida screening platform for pregnant women.
Massively Parallel Sequencing of Genes Implicated in Heritable Cardiac Disorders: A Strategy for a Small Diagnostic Laboratory

Sudden cardiac death (SCD) in people before the age of 35 years is a devastating event for any family. The causes of SCD in the young can be broadly divided into two groups: heritable cardiac disorders that affect the heart structure (cardiomyopathies) and primary electrical disorders (cardiac ion channelopathies). Genetic testing is vital as those suffering from cardiac ion channelopathies have structurally normal hearts, and those with cardiomyopathies may only show subtle abnormalities in the heart and these signs may not be detected during an autopsy. Post-mortem genetic testing of SCD victims is important to identify the underlying genetic cause. This is important as family cascade screening may be undertaken to identify those who may be at risk and provide vital information about risk stratification and clinical management. The development of massively parallel sequencing (MPS) has made it possible for the simultaneous screening of multiple patients for hundreds of genes. In light of this, we opted to develop an MPS approach for SCD analysis that would allow us to screen for mutations in genes implicated in cardiomyopathies and cardiac ion channelopathies. The rationale behind this panel was to limit it to genes carrying the greatest mutation load. If no likely pathogenic gene variant were found then testing could cascade to whole exome/genome sequencing as a gene-discovery exercise. The overarching aim was to design and validate a custom-cardiac panel that satisfies the diagnostic requirements of LabPLUS (Auckland City Hospital, Auckland, NZ) and the guidelines provided by the Royal College of Pathologists of Australasia and the Association for Clinical Genetic Science.
Radiolabeled Antibodies Against Müllerian-Inhibiting Substance Receptor, Type II: New Tools for a Theranostic Approach in Ovarian Cancer

We have developed the 16F12 mouse monoclonal antibody (mAb), which targets the Müllerian-inhibiting substance receptor, type II (MISRII), expressed by ovarian tumors. Here, we assessed in preclinical models the possibility of using radiolabeled 16F12 in a theranostic approach for small-volume ovarian peritoneal carcinomatosis, such as after cytoreductive surgery. Methods: DOTA-, DTPA- or deferoxamine mesylate–conjugated 16F12 mAb was radiolabeled with β-particle (177Lu) or α-particle (213Bi) emitters for therapeutic use and with 89Zr for PET imaging. On the 13th postxenograft day, mice bearing intraperitoneal MISRII-positive AN3CA endometrial carcinoma cell xenografts were treated by conventional intraperitoneal radioimmunotherapy (IP-RIT) with 10 MBq of 177Lu-16F12 or 12.9 MBq of 213Bi-16F12 or by brief intraperitoneal radioimmunotherapy (BIP-RIT) using 50 MBq of 177Lu-16F12 or 37 MBq of 213Bi-16F12. For BIP-RIT, 30 min after injection of the radiolabeled mAbs, the peritoneal cavity was washed to remove the unbound radioactivity. The biodistribution of 177Lu- and 213Bi-16F12 mAbs was determined and then used for dose assessment. Hematologic toxicity was also monitored. Results: The 16F12 mAb was satisfactorily radiolabeled for both therapy and imaging. IP-RIT with 177Lu-16F12 was slightly more efficient in delaying tumor growth than IP-RIT with 213Bi-16F12. Conversely, 213Bi-16F12 was more efficient than 177Lu-16F12 in BIP-RIT. The biodistribution analysis showed that the tumor-to-blood uptake ratio was significantly higher with BIP-RIT than with IP-RIT for both 213Bi- and 177Lu-16F12. Hematologic toxicity was more pronounced with 177Lu-16F12 than with 213Bi-16F12. SPECT/CT images (after BIP-RIT with 177Lu-16F12) and PET/CT images (after injection of 89Zr-16F12 in the tail vein) showed focal uptake at the tumor site. Conclusion: Radiolabeled 16F12 could represent a new theranostic tool for small-volume ovarian peritoneal carcinomatosis. Specifically, 213Bi-16F12–based BIP-RIT could be proposed to selected patients as an alternative adjuvant treatment immediately after cytoreductive surgery. An anti-MISRII mAb is currently being used in a first-in-human study, thus making radiolabeled anti-MISRII mAbs a realistic theranostic option for the clinic.
Random coupling model for wireless communication channels

In this paper, we derive an explicit formula for the voltage-to-voltage transfer function of multiple-input multiple-output (MIMO) wireless channels. A statistical model, the random coupling model, is used to develop the open-circuit transfer function of the MIMO channel on a physical basis. The emulation of realistic wireless channels is typically performed through irregular cavities with high losses. In this case, we find that the transfer function takes a simple form involving the free-space impedance matrix of antennas and a fluctuation matrix expressing the wave chaos inside the environment. Monte Carlo simulations of the open-circuit transfer function are performed for MIMO systems up to three antennas in the transmit and receive arrays. In contrast to the common assumption that the MIMO channel fulfill multivariate normality (MVN), the Hans-Zinckler test of the obtained ensembles of the MIMO channel show that the MVN assumption of the MIMO channel tends to be invalid with an increasing number of antennas in the transmitting and receiving arrays, when mutual coupling is present in the arrays. Numerical results indicate that this effect is more pronounced at relatively low frequencies.
Nationwide French Study of RET Variants Detected from 2003 to 2013 Suggests a Possible Influence of Polymorphisms as Modifiers.

BACKGROUND The presence of single nucleotide polymorphisms (SNPs) in the REarranged during Transfection (RET) gene has been investigated with regard to their potential role in the development or progression of medullary thyroid cancer or pheochromocytomas (PHEO) in patients with the multiple endocrine neoplasia type 2 (MEN2) syndrome. The aim of this study was to evaluate the spectrum of RET variants in France between 2003 and 2013, and to evaluate the impact of SNPs on the MEN2 A phenotype. METHODS In this retrospective cohort study, RET variants were screened in 5109 index cases, and RET pathogenic variants were screened in 2214 relatives. Exons 5, 8, 10, 11, 13, 14, 15, and 16 were characterized by Sanger sequencing. RET pathogenic variants, RET variants with unknown functional significance (VUS), and four RET SNP variants-G691S (rs1799939), L769L (rs1800861), S836S (rs1800862), and S904S (rs1800863)-were characterized and are reported in index cases. In silico analysis and classification following the recommendation of the American College of Medical Genetics and Genomics was performed for RET VUS. Each patient's age at the time of diagnosis, sex, and the endocrine neoplasias present at molecular diagnosis were recorded. RESULTS Twenty-six single VUS in RET without any well-defined risk profiles were found in 33 patients. Nine of these were considered probably pathogenic, 11 of uncertain significance, and six as probably benign. Three double pathogenic variants found in three patients were classified as pathogenic. A study of the entire cohort showed that patients carrying pathogenic variants or VUS in RET together with PHEO were diagnosed earlier than the others. The presence of the G691S SNP, or a combination of SNPs, increased the risk of developing PHEO but did not modify the date of the diagnosis. No association was found between SNPs and medullary thyroid cancer or hyperparathyroidism. CONCLUSIONS The findings propose a classification of 15 of the 26 VUS in RET without any well-defined risk profiles and suggest that the G691S SNP, or a combination of SNPs, may be associated with the development of PHEO.
Different Dietary Levels of Protein to Lipid Ratio Affected Digestive Efficiency, Skeletal Growth, and Muscle Protein in Rainbow Trout Families

Normal Protein (NP) and High Protein (HP) diets were provided to rainbow trout families from juvenile to maturity. Muscle protein concentration increased during growth and the protein to lipid (P/L) ratio was doubled at late stage with respect to the dietary P/L ratio. The HP-diet fish showed higher protein deposition in body and white muscle, and had lower condition factor due to protein deposition associated more with body length than body weight. Fish growth rates were decreased at maturation, and the HP-diet fish showed lower growth rate including the activity ratio of trypsin to chymotrypsin (T/C ratio) and feed efficiency. Trypsin and chymotrypsin specific activities were related to dietary protein levels, and the T/C ratio was related to intestinal weight and growth rate independent of the enzymes specific activity levels. The families with high growth capacity could double increase white muscle P/L ratio levels, compared to low and medium growth families, if they were fed on HP-diet. The digestive enzyme extracts from high growth families resulted in higher in vitro protein digestibility for all diets. The effect of dietary P/L ratio on digestion, skeletal growth (length) and the white muscle P/L ratio in fish with different growth capacities is illustrated.
Compressive Behavior of Sustainable Steel-FRP Composite Bars with Different Slenderness Ratios

This paper presents experimental studies on the compressive behavior of a sustainable steel-fiber reinforced composite bar (SFCB) under uniaxial compressive loading. The SFCB, combined with steel and fiber reinforced polymer (FRP), is expected to significantly enhance structural safety and sustainability. A new test method with LVDT and extensometer sensors was developed and verified through experiments to test the tensile and compressive behavior of the SFCB. Fifty-four specimens including SFCB and inner steel bar (ISB) with different slenderness ratios were tested. The test results indicated that the initial compressive elastic modulus of the SFCB was essentially the same as its initial tensile elastic modulus. The compressive yield load of the SFCB was essentially irrelevant to the slenderness ratio, and the ultimate compressive stress of the SFCBs varied inversely with the slenderness ratios. The squash load of the SFCB tended to be conservative for predicting the compressive yield load of the SFCB, while the equivalent critical global buckling load of the SFCB was much higher than its corresponding compressive yield load and ultimate load due to the inelastic buckling mechanism of the SFCB within the range of the equivalent slenderness ratios studied in this paper.
A novel approach to the synthesis of photoluminescent germanium nanoparticles by reactive laser ablation

We propose a novel approach to the synthesis of photoluminescent Ge nanoparticles using reactive laser ablation. The deposition of germanium under low oxygen pressure (2–100 mTorr) yields Ge nanoparticles embedded in a Ge oxide matrix. Ge nanostructures were characterized by transmission electron microscopy, x-ray photoelectron spectroscopy and photoluminescence (PL) spectroscopy. The oxygen pressure determines the degree of oxidation of GeOx films (0≤x≤2). A strong PL signal depending on oxygen pressure was observed. The PL maximum is detected for 10 mTorr of oxygen corresponding to a nanoparticle size of 1.8 ± 0.5 nm.
Jianpi Bushen, a Traditional Chinese Medicine Therapy, Combined with Chemotherapy for Gastric Cancer Treatment: A Meta-Analysis of Randomized Controlled Trials

Objective. To investigate the effects of Jianpi Bushen (JPBS), a traditional Chinese medicine that is used to invigorate the spleen and tonify the kidney, combined with chemotherapy for the treatment of gastric cancer. Methods. Literature retrieval was performed in PubMed, EMBASE, Cochrane Library, MEDLINE, CNKI, Wanfang Data Information Site, and VIP from inception to October 2017. Randomized controlled trials to evaluate the effects of JPBS combined with chemotherapy were identified. The primary reported outcomes were KPS (Karnofsky Performance Status), clinical curative efficiency, immune function, blood system, and nonhematologic system. Review Manager 5.3 (RevMan 5.3) was used for data analysis, and the quality of the studies was also appraised. Results. A total of 26 studies were included with 3098 individuals. The results of the meta-analysis indicated that treatment of gastric cancer with the combination of JPBS and chemotherapy resulted in better outcomes compared to chemotherapy alone. Conclusion. Evidence from the meta-analysis suggested that JPBS combined with chemotherapy has a positive effect on gastric cancer treatment. However, additional rigorously designed and large sample randomized controlled trials are required to confirm the efficacy and safety of this treatment.
The Cost of Climate Change in Tanzania: Impacts and Adaptations

In recent years, Tanzania has witnessed a number of climate related disasters namely, flooding, droughts, widespread crop failures, livestock deaths and intensification of climate sensitive diseases among others. Regular climate discussions in the country have often underestimate crucial problems related to climate change like chronic energy crisis and influx of people to urban areas arguably because of failing agriculture in farming areas. This paper has highlighted the fact that the poor, usually with limited resources and who contribute the least to the causes of climate change are the most affected in many ways. In addition, it gives a detailed account of the issues of climate change in Tanzania and explains the effects of climate variability using examples. The effects of climate change in the country are widespread and significantly interfere with agriculture, while at the same time, reducing the ability of the society to deliver services. Indigenous knowledge such as survival skills and coping mechanisms adopted by different societies in Tanzania, have been discussed in the paper. Such coping mechanisms however are overwhelmed by the impacts of climate change on the people. Different institutions including the government have taken some positive steps towards combating climate change; however the efforts done so far are insufficient. In addition, addressing climate change in Tanzania is hindered with inadequate resources, corruption and poor coordination and implementation of combating measures. Generally current climate variability in the nation is an issue of concern for all future plans and must be addressed. (Journal of American Science 2010; 6(3):182-196). (ISSN: 1545-1003).
The case for case studies in management research

It is commonly asserted that qualitative research in the organizational sciences lacks the rigor and objectivity of the quantitative approach. Case studies, while commonly used for educational purposes, have been viewed in a less favorable light in terms of research. This paper suggests that case studies represent an important research track in organizational science, not only as a method of generating hypotheses for quantitative studies, but for generating and testing theory. The paper will develop arguments in support of case study research, will highlight particular issues and constraints relating to case study research, and will offer recommendations for the use of this method.
Corporation Income Taxes and the Cost of Capital: A Revision

The value of debt tax shields in foundational corporate valuation models by Nobel Laureates Modigliani and Miller (MM) continues to be a controversial issue that is central to our understanding of corporate finance. Rather than discounting debt interest payments using a riskless interest rate or unlevered equity rate, the present paper proposes the use of the levered cost of equity. Assuming no bankruptcy risk and no personal taxes, our revised tax model yields an inverted U-shaped firm value function with an interior optimal capital structure. Analyses are extended to Miller’s personal tax extension of MM’s tax model. Also, implications to corporate capital structure decisions and previous literature are discussed.
Molecular Ageing of Alpha- and Beta-Synucleins: Protein Damage and Repair Mechanisms

Abnormal α-synuclein aggregates are hallmarks of a number of neurodegenerative diseases. Alpha synuclein and β-synucleins are susceptible to post-translational modification as isoaspartate protein damage, which is regulated in vivo by the action of the repair enzyme protein L-isoaspartyl O-methyltransferase (PIMT). We aged in vitro native α-synuclein, the α-synuclein familial mutants A30P and A53T that give rise to Parkinsonian phenotypes, and β-synuclein, at physiological pH and temperature for a time course of up to 20 days. Resolution of native α-synuclein and β-synuclein by two dimensional techniques showed the accumulation of a number of post-translationally modified forms of both proteins. The levels of isoaspartate formed over the 20 day time course were quantified by exogenous methylation with PIMT using S-Adenosyl-L-[3H-methyl]methionine as a methyl donor, and liquid scintillation counting of liberated 3H-methanol. All α-synuclein proteins accumulated isoaspartate at ∼1% of molecules/day, ∼20 times faster than for β-synuclein. This disparity between rates of isoaspartate was confirmed by exogenous methylation of synucleins by PIMT, protein resolution by one-dimensional denaturing gel electrophoresis, and visualisation of 3H-methyl esters by autoradiography. Protein silver staining and autoradiography also revealed that α-synucleins accumulated stable oligomers that were resistant to denaturing conditions, and which also contained isoaspartate. Co-incubation of approximately equimolar β-synuclein with α-synuclein resulted in a significant reduction of isoaspartate formed in all α-synucleins after 20 days of ageing. Co-incubated α- and β-synucleins, or α, or β synucleins alone, were resolved by non-denaturing size exclusion chromatography and all formed oligomers of ∼57.5 kDa; consistent with tetramerization. Direct association of α-synuclein with β-synuclein in column fractions or from in vitro ageing co-incubations was demonstrated by their co-immunoprecipitation. These results provide an insight into the molecular differences between α- and β-synucleins during ageing, and highlight the susceptibility of α-synuclein to protein damage, and the potential protective role of β-synuclein.
Inside a Cucuteni Settlement: Remote Sensing Techniques for Documenting an Unexplored Eneolithic Site from Northeastern Romania

This paper presents recent results of an integrated non-invasive investigation carried out in a previously unexplored settlement from northeastern Romania, belonging to the last great Eneolithic civilisation of Old Europe, the Cucuteni-Trypillia cultural complex. Although there is a long history of research concerning this culture, at only a handful of sites has archaeological research completed a comprehensive planimetric image. This makes it impossible to determine a typological evolution of the internal organisation of Cucutenian sites, both diachronically, across the three great phases of the culture (A, A−B and B for the Romanian area), and spatially, from SE Transylvania to the Republic of Moldova, and towards the steppes of the Ukraine. Accordingly, in certain environmental conditions, many essential behavioural aspects of Cucutenian communities are far from understood. Consequently, the generalisation and integration of non-invasive prospecting methods—Light Detection and Ranging (LiDAR), aerial photography, earth resistivity, magnetometry, and their integration through Geographic Information System (GIS)—clearly represents a feasible alternative for deciphering the Cucuteni culture. These complementary investigation methods were applied for this case study, emphasis being put on the conjoint use of datasets from each technique. On the basis of results recently obtained from the Războieni–Dealul Mare/Dealul Boghiu site, innovative characteristics are described concerning intra-site spatial organisation, a typology of the fortification systems, the existence of ritual or delimitation ditches, and the presence of habitations outside fortified areas.
Study the Influence of UV-Irradiation on the Photo Stability of Pure and Plasticized Poly (4-Vinyl Biphenyl) in Solid Films

The effect of UV irradiation and blending with phthalate and terephthalate plasticizers on the photo-stability of Poly (4-vinyl biphenyl) was studied at different intervals of irradiation time and in presence of air. The increase in irradiation time on the photodegradation of polymer thin films caused a change in the intensity and shape of the fluorescence band. It has been found that the stability of the polymer decreases with the increase of irradiation time, and to increases with the increase of the amount of added phthalate and terephthalate plasticizers, which is evidence of polymer photodegradation. The FT-IR spectra of irradiated pure and blended polymer with phthalate and terephthalate plasticizers showed a decrease in some absorption bands and increase in the other bands, this is also another factor for the occurrence of photo degradation of the irradiated polymer. The increase in the intensity of absorption of carbonyl and hydroxyl region, indicates a possible photogegradation of polymeric chains and the formation of alcohols, aliphatic ketones and to the increase in the number of (C=C) that resulted from hydrogen abstraction during chains-scission.