Miracle-dz/UniFAR-FTUs huggingface dataset:
The basal keratin network of stratified squamous epithelia: defining K15 function in the absence of K14

Keratin 5 and keratin 14 have been touted as the hallmarks of the basal keratin networks of all stratified squamous epithelia. Absence of K14 gives rise to epidermolysis bullosa simplex, a human blistering skin disorder involving cytolysis in the basal layer of epidermis. To address the puzzling question of why this disease is primarily manifested in skin rather than other stratified squamous epithelia, we ablated the K14 gene in mice and examined various tissues expressing this gene. We show that a key factor is the presence of another keratin, K15, which was hitherto unappreciated as a basal cell component. We show that the levels of K15 relative to K14 vary dramatically among stratified squamous epithelial tissues, and with neonatal development. In the absence of K14, K15 makes a bona fide, but ultrastructurally distinct, keratin filament network with K5. In the epidermis of neonatal mutant mice, K15 levels are low and do not compensate for the loss of K14. In contrast, the esophagus is unaffected in the neonatal mutant mice, but does appear to be fragile in the adult. Parallel to this phenomenon is that esophageal K14 is expressed at extremely low levels in the neonate, but rises in postnatal development. Finally, despite previous conclusions that the formation of suprabasal keratin filaments might depend upon K5/K14, we find that a wide variety of suprabasal networks composed of different keratins can form in the absence of K14 in the basal layer.
Mechanisms of Adherence in a Harm Reduction Housing Program

Advances in clinical therapies for people living with HIV have greatly increased life expectancies, but the high levels of adherence that are necessary for clinical success are infrequently achieved, especially for chronically homeless substance users. The Open Door is a harm reduction housing program that seeks to improve clinical outcomes for this population. We present findings from qualitative interviews with residents of the program, conducted to explore facilitators of residents’ success in the program, which is ultimately defined as HIV clinical adherence. Two major themes developed. Positive changes captures processes or characteristics that residents reported as having changed as a result of living in the program. Mechanisms of success reflects aspects of the program to which residents attributed their improved adherence. Given that improving adherence in vulnerable populations has both clinical and public health implications, our study suggests future avenues for research and foundations on which to build future interventions.
Peer relationships in boys with attention-deficit hyperactivity disorder with and without comorbid aggression

Abstract Peer relationship difficulties and peer rejection are common in youngsters with attention-deficit hyperactivity disorder (ADHD), mandating focus on assessment issues, underlying reasons for peer approval and disapproval, links with comorbid aggression, and the mediating role of sociocognitive mechanisms as well as emotion regulation strategies. With previously unfamiliar ADHD and comparison boys who attended naturalistic summer research programs, we found the following: (a) parent and teacher estimates (but not self-reports) showed moderate correspondence with peer-nominated social preference; (b) ADHD boys were more likely than their comparison peers to accept other ADHD agemates; (c) aggression and noncompliance Predominated as self-reported reasons for rejecting agemates in both ADHD and comparison groups, with Perceived similarity the chief mediator of peer acceptance; (d) the high-aggressive subgroup of ADHD boys showed markedly worse peer sociometric status than did ADHD boys without aggression, for whom social isolation was also a predictor of peer reputation; and (e) self-reported social goals of a sensation-seeking nature and observed emotional reactivity characterized high-aggressive boys with ADHD and predicted end-of-program peer disapproval. We discuss implications for normal developmental processes and for intervention efforts.
Conceptions of knowledge in research on students' understanding of the greenhouse effect: Methodological positions and their consequences for representations of knowing

Much of the research on students' understanding of the greenhouse effect and global warming reports poor results. Students are claimed to hold misconceptions and naive beliefs, and the impact of teaching on their conceptions is also low. In the present study, these results are called into question, and it is argued that they may to a large extent be seen as artifacts of the research methods deployed, in particular when written questionnaires are used. When following students' project work in school over a long period, many of the misunderstandings reported in the literature do not appear. It is argued that the appropriation and use of scientific language when discussing complex socioscientific issues is a gradual process. When observing the language and mediational means students use over time, it is obvious that they are able to identify and use central distinctions in their interactions. They are also able to make productive use of texts and other materials that allow them to successively approximate scientific modes of reasoning. Thus, what students know emerges in communicative practices where they interact with others and with cultural tools in a focused activity. It is argued that students' knowledge of complex multidisciplinary phenomena of this kind may be particularly ill-suited to conventional questionnaire types of testing. © 2009 Wiley Periodicals, Inc. Sci Ed93:978–995, 2009
The effect of dietary sodium bicarbonate on performance and blood parameters of broiler chickens and local balady breed inoculated with Salmonella gallinarum.

To determine the role of 0.5% sodium bicarbonate (NaHCO 3 ) on the performance of chickens inoculated with Salmonella gallinarum, a total of 720 one-day-old-broiler chickens from four strains Hubbard (H), Lohmann (L), Ross (R) and Balady (B) were grown for 42 days in 24 pens, two levels of NaHCO 3 (0 and 0.5%) and 3 replicates/treatment (4 strains × 2 dietary treatment × 3 replicates of 30 birds for each group) in a completely randomized design. Dietary NaHCO 3 supplementation had significant adverse effects on average body weight (P<0.001), feed intake and feed conversion ratio (FCR). Body weight of the four non-treated groups was significantly higher than treated (P<0.001) and B strain birds had the lightest final body weight and the poorest FCR and was significantly different from the 3 commercial strains (P<0.05). Blood glucose, cholesterol, and total protein were not affected by dietary alterations or strain differences. The re-isolation of Salmonella gallinarum from liver and intestine of sacrificed and inoculated chickens revealed that the organism could be shed from liver and intestine with alternative rates in all strains. There was a significantly higher mortality rate in treated groups (P<0.05) during the whole experimental period. Furthermore, B strain was numerically the most resistant to Salmonella gallinarum of the untreated groups. The use of 0.5% dietary NaHCO 3 did not improve the performance of the different strains nor prevent the localization of Salomenella gallinarum.
The Potsdam Commentary Corpus

A corpus of German newspaper commentaries has been assembled and annotated with different information (and currently, to different degrees): part-of-speech, syntax, rhetorical structure, connectives, co-reference, and information structure. The paper explains the design decisions taken in the annotations, and describes a number of applications using this corpus with its multi-layer annotation.
You Are What You Tweet: Connecting the Geographic Variation in America’s Obesity Rate to Twitter Content

We conduct a detailed investigation of the relationship among the obesity rate of urban areas and expressions of happiness, diet and physical activity on social media. We do so by analyzing a massive, geo-tagged data set comprising over 200 million words generated over the course of 2012 and 2013 on the social network service Twitter. Among many results, we show that areas with lower obesity rates: (1) have happier tweets and frequently discuss (2) food, particularly fruits and vegetables, and (3) physical activities of any intensity. Additionally, we provide evidence that each of these results offer different and unique insight into the variation of the obesity rate in urban areas within the United States. Our work shows how the contents of social media may potentially be used to estimate real-time, population-scale measures of factors related to obesity.
Molecular basis for MMP9 induction and disruption of epithelial cell–cell contacts by galectin-3

ABSTRACT Dynamic modulation of the physical contacts between neighboring cells is integral to epithelial processes such as tissue repair and cancer dissemination. Induction of matrix metalloproteinase (MMP) activity contributes to the disassembly of intercellular junctions and the degradation of the extracellular matrix, thus mitigating the physical constraint to cell movement. Using the cornea as a model, we show here that a carbohydrate-binding protein, galectin-3, promotes cell–cell detachment and redistribution of the tight junction protein occludin through its N-terminal polymerizing domain. Notably, we demonstrate that galectin-3 initiates cell–cell disassembly by inducing matrix metalloproteinase expression in a manner that is dependent on the interaction with and clustering of the matrix metalloproteinase inducer CD147 (also known as EMMPRIN and basigin) on the cell surface. Using galectin-3-knockout mice in an in vivo model of wound healing, we further show that increased synthesis of MMP9 at the leading edge of migrating epithelium is regulated by galectin-3. These findings establish a new galectin-3-mediated regulatory mechanism for induction of metalloproteinase expression and disruption of cell–cell contacts required for cell motility in migrating epithelia.
Skin benefits of a myconoside‐rich extract from resurrection plant Haberlea rhodopensis

Resurrection plant Haberlea rhodopensis develops molecules to survive drought stress. These molecules allow the plant to resurge from a desiccation state. We have extracted a specific fraction from the plant (Haberlea extract) and found it rich, among other molecules, of a caffeoyl phenylethanoid glycoside called myconoside, a molecule extremely abundant in the plant with a potential role in survival. Peroxide‐stressed normal human dermal fibroblasts treated with the Haberlea extract, showed increased collagen VI (+822%), collagen XVI (+928%) and elastin (+144%) mRNA synthesis, measured by RT‐qPCR. This effect was superior to those obtained with benchmarks retinoic acid and retinol. When used at 3% in human skin biopsies, Haberlea extract protected against UV‐induced dermis oxidation by 100% (P < 0.01), as evidenced by immunohistochemistry. Finally, when tested in human volunteers (n = 20) at 3% in a cream against a placebo, Haberlea extract increased skin elasticity (3× placebo, P < 0.0002) and skin radiance (4× placebo, P < 0.05) after only 15 days of treatment, with the effect sustained after 30 and 60 days of treatment. We demonstrated that by using Haberlea extract (particularly rich in glycoside myconoside), it is possible to strongly stimulate antioxidant skin defences and extracellular matrix protein synthesis. This effect, in turn, will further stimulate skin elasticity and skin radiance significantly in human volunteers. The extract can be suggested for anti‐ageing treatments, intended for claims such as protection from oxidation, increased skin elasticity and enhanced skin radiance.
Accelerated axon outgrowth, guidance, and target reinnervation across nerve transection gaps following a brief electrical stimulation paradigm.

OBJECT Regeneration of peripheral nerves is remarkably restrained across transection injuries, limiting recovery of function. Strategies to reverse this common and unfortunate outcome are limited. Remarkably, however, new evidence suggests that a brief extracellular electrical stimulation (ES), delivered at the time of injury, improves the regrowth of motor and sensory axons. METHODS In this work, the authors explored and tested this ES paradigm, which was applied proximal to transected sciatic nerves in mice, and identified several novel and compelling impacts of the approach. Using thy-1 yellow fluorescent protein mice with fluorescent axons that allow serial in vivo tracking of regeneration, the morphological, electrophysiological, and behavioral indices of nerve regrowth were measured. RESULTS The authors show that ES is associated with a 30%-50% improvement in several indices of regeneration: regrowth of axons and their partnered Schwann cells across transection sites, maturation of regenerated fibers in gaps spanning transection zones, and entry of axons into their muscle and cutaneous target zones. In parallel studies, the authors analyzed adult sensory neurons and their response to extracellular ES while plated on a novel microelectrode array construct designed to deliver the identical ES paradigm used in vivo. The ES accelerated neurite outgrowth, supporting the concept of a neuron-autonomous mechanism of action. CONCLUSIONS Taken together, these results support a robust role for brief ES following peripheral nerve injuries in promoting regeneration. Electrical stimulation has a wider repertoire of impact than previously recognized, and its impact in vitro supports the hypothesis that a neuron-specific reprogrammed injury response is recruited by the ES protocol.
A space-optimal data-stream algorithm for coresets in the plane

Given a point set P⊆R<sup>2</sup>, a subset Q⊆ P is an ε-kernel of P if for every slab W containing Q, the (1+ε)-expansion of W also contains P. We present a data-stream algorithm for maintaining an ε-kernel of a stream of points in R<sup>2</sup> that uses O(1/√ ε) space and takes O(log (1/ε)) amortized time to process each point. This is the first space-optimal data-stream algorithm for this problem.
Endometrial destruction techniques for heavy menstrual bleeding: a Cochrane review.

UNLABELLED This paper is based on a Cochrane review published in The Cochrane Library, issue 3, 2002 (see www.CochraneLibrary.net for information) with permission from The Cochrane Collaboration and Update Software. Cochrane reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and The Cochrane Library should be consulted for the most recent version of the review. BACKGROUND The objective of this review was to compare the efficacy, safety and acceptability of methods used to destroy the endometrium to reduce heavy menstrual bleeding (HMB) in premenopausal women. METHODS We searched the Cochrane Controlled Trials Register, Medline, Embase, Current Contents, Biological Abstracts, Psyclit and CINAHL. We also searched the specialized register of the Cochrane Menstrual Disorders and Subfertility Group and reference lists of articles, and contacted pharmaceutical companies and experts in the field. Randomized controlled trials comparing endometrial ablation techniques in women with a complaint of HMB without uterine pathology were selected. The outcomes included reduction of HMB, improvement in quality of life, operative outcomes, satisfaction with outcome, complications and need for further surgery. The two reviewers independently selected trials for inclusion, assessed trials for quality and extracted data. Attempts were made to contact authors for clarification of data in some trials. Adverse events were only assessed if they were separately measured in the included trials. RESULTS In comparing hysteroscopic techniques, the vaporizing electrode procedure was less difficult to perform [odds ratio (OR) = 0.25; 95% confidence interval (CI): 0.1, 0.7] and had less fluid deficit [weighted mean difference (WMD) = -258 ml; 95% CI: -342.1, -174.0] than transcervical resection of the endometrium (TCRE). The odds of fluid overload and equipment failure were higher (OR = 5.2; 95% CI: 1.5, 18.4 and OR = 6.0; 95% CI: 1.7, 20.9 respectively) for those women having laser treatment as compared with TCRE. In comparing traditional hysteroscopic endometrial ablation with the newer second generation techniques overall, the newer techniques took less time to perform (WMD = -11 min; 95% CI: -18.6, -2.6) and were more likely to be performed under local anaesthesia (OR = 7.6; 95% CI: 1.1, 52.7) but had a greater chance of equipment failure (OR = 4.1; 95% CI: 1.1, 15.0). The reduction in HMB did not differ significantly between any of the groups. CONCLUSIONS The rapid development of a number of new methods of endometrial destruction has made systematic comparisons between methods and with the 'gold standard' of TCRE difficult. Most of the newer techniques are performed blind and are technically easier than hysteroscopy-based methods. Overall, the existing evidence suggests that success rates and complication profile of newer techniques of ablation compare favourably with TCRE, although technical difficulties with new equipment need to be ironed out.
A comparison of the psychometric effects of remoxipride with those of haloperidol, thioridazine, and lorazepam in healthy volunteers

In this placebo and verum (lorazepam 2 mg) controlled double‐blind crossover study the acute effects of Remoxipride 50 mg and 100 mg; Haloperidol 2·5 mg; and Thioridazine 50 mg were examined in 18 healthy volunteers. CNS function was assessed using a battery of psychometric measures, viz: Critical Flicker Fusion Threshold (CFFT); Choice Reaction Time (CRT); Tracking Accuracy (RMS) and Peripheral Reaction Time (PRT); Sternberg Memory Scanning (SMS); Word Memory (WM) as well as Line Analogue Ratings of subjective sedation (LARS) and the Leeds Sleep Evaluation Questionnaire (LSEQ). Both doses of remoxipride were associated with a reduction of CFFT: 0·9 Hz for 50 mg and 1·3 Hz for 100 mg. The 100 mg dose caused an increase (impairment) in CRT total time of 38 msec. Haloperidol reduced CFFT, while thioridazine and lorazepam impaired performance on the majority of objective measures, as expected for known sedative drugs. Thioridazine led to hypotension in three subjects, and to subjective sedation (LARS), but no treatment affected responses on the LSEQ administered the morning of the day following treatment. These results show that remoxipride has some depressant effects on CNS function, but that these are less prominent than those of sedative neuroleptics such as thioridazine.
The Non-Anglophone Scholar on the Periphery of Scholarly Publication.

As a symptom of globalization and the marketization of the universities, more and more scholars, many or most, of whom use English as an additional language (EAL), are being required to published in English. This article presents some qualitative data which highlights some of the difficulties encountered by such writers. It first discusses a previously published case study of an EAL writer writing for publication, highlighting some of the difficulties encountered by this young scholar. It then goes on to consider a particular writing strategy adopted by some EAL writers which might be considered to be controversial, the copying of fragments of text from previously published work, and referred to here as language reuse. The final part of the paper discusses various approaches directed towards alleviating problems encountered by EAL writers such as those exemplified in the main body of the paper.
User readiness for IS development: An examination of 50 cases

Nearly every information system development (ISD) triggers a wide variety of feelings from all stakeholders who are impacted in various ways because, more than ever, ISD is leveraged as a means of organizational change. Motivated by the stubborn progress on the success rate of ISD, this research, in contrast to the previous research, hypothesizes that user readiness underpins all other kinds of readiness for ISD. In light of this hypothesis, our research team has interviewed representatives of 50 ISD projects in order to identify the efficacy of user readiness in the success rate of ISD and the responsiveness of user readiness to project-specific efforts. Our interview findings indicate that user readiness does correlate to the success of ISD, although such correspondence varies in degree according to the complexity of ISD. In addition, our findings show that the motivational aspect of user readiness responds to project-specific efforts more slowly than the cognitive aspect of it, and that user readiness is in the midst of other kinds of readiness such as process readiness, data readiness, organizational and cultural readiness, so it is pivotal to the success of ISD. The findings from this research should help researchers and practitioners refocus their efforts to improve the success rate of ISD. Copyright © 2008 John Wiley & Sons, Ltd.
Immunocytochemical Characterization of Delta-Opioid and Mu-Opioid Receptor Protein in the Bovine Pineal Gland

Opioidergic innervation has been identified in the mammalian pineal gland. Recently, opioid receptors in bovine pineal glands have been characterized; the activation of these receptors leads to the stimulation of melatonin synthesis. In this study, the precise localization of opioid receptors in bovine pineal glands was determined by an immunohistochemical technique using antibodies raised against delta-opioid and mu-opioid receptors. Immunoreactivity of these two receptors was present at a moderate level in pinealocytes. A double-labeling study has shown that delta-opioid receptors are localized predominantly with mu-opioid receptors in the same pinealocytes. These immunopositive pinealocytes are often located in a group; however, some of them are dispersed individually. In addition, both types of receptors were found in glial cells and processes. A small number of delta-receptor-immunoreactive nerve fibers were observed in the perivascular space and intraparenchyma of the pineal gland. Mu-opioid receptor immunoreactivity was found in a number of nerve fibers throughout the gland, and in terminal-like dots on pinealocytes. There was immunocolocalization between delta-opioid receptors or mu-opioid receptors and leu-enkephalin in some nerve fibers. The results of this study indicate that the modulatory effect of the opioid system on melatonin secretion in pineal glands might act via opioid receptors on pinealocytes, whereas receptors located on nerve fibers might modulate the release of opioid peptides.
Effects of Simultaneous or Sequential Weight Loss Diet and Aerobic Interval Training on Metabolic Syndrome

Abstract Our purpose in this study was to investigate efficient and sustainable combinations of exercise and diet-induced weight loss (DIET), in order to combat obesity in metabolic syndrome (MetS) patients. We examined the impact of aerobic interval training (AIT), followed by or concurrent to a DIET on MetS components. 36 MetS patients (54±9 years old; 33±4 BMI; 27 males and 9 females) underwent 16 weeks of AIT followed by another 16 weeks without exercise from the fall of 2013 to the spring of 2014. Participants were randomized to AIT without DIET (E CON, n=12), AIT followed by DIET (E-then-D, n=12) or AIT concurrent with DIET (E+D, n=12) groups. Body weight decreased below E CON similarly in the E-then-D and E+D groups (~5%). Training improved blood pressure and cardiorespiratory fitness (VO2peak) in all groups with no additional effect of concurrent weight loss. However, E+D improved insulin sensitivity (HOMA) and lowered plasma triglycerides and blood cholesterol below E CON and E-then-D (all P<0.05). Weight loss in E-then-D in the 16 weeks without exercise lowered HOMA to the E+D levels and maintained blood pressure at trained levels. Our data suggest that a new lifestyle combination consisting of aerobic interval training followed by weight loss diet is similar, or even more effective on improving metabolic syndrome factors than concurrent exercise plus diet.