Miracle-dz/UniFAR-FTUs huggingface dataset:
Fuchs Endothelial Corneal Dystrophy: A Systematic Immunofluorescence Study of Collagen Type VIII Suggests Heterogeneous Pathophysiology

Purpose: The purpose of this study was to investigate the pathophysiologic heterogeneity of Fuchs endothelial corneal dystrophy (FECD). Methods: We conducted a systematic immunofluorescence study on 39 Descemet membrane samples from FECD patients and compared these with 10 Descemet membrane samples from patients with pseudophakic bullous keratopathy (PBK) and 7 normal corneas. Samples were analyzed with immunofluorescence using antibodies to the &agr;1-chain [collagen VIII &agr;1-chain (COL8A1)] and &agr;2-chain (COL8A2). Intensity of staining was assessed using a subjective grading scale from 0 to 3. The presence of specific staining patterns was noted. Results: The overall distribution of COL8A1 staining intensity between groups was significantly different (P = 0.002). There was marked/intense staining in 85% (33/39) of the FECD samples, 40% (4/10) of the PBK samples (P = 0.034), and 29% (2/7) of normal samples (P = 0.004). The overall distribution of COL8A2 staining intensity was not significantly different between groups (P = 0.39). There was marked/intense staining in 33% (13/39) of the FECD samples, 10% (1/10) of PBK samples, and 14% (1/7) of the normal samples. There was substantial variation in staining intensity in the FECD group, a phenomenon that was especially pronounced for the COL8A2 antibody. Conclusions: We found increased staining for COL8A1, but not COL8A2 in FECD samples. Further, there was striking variation of staining intensity in FECD patients, indicating pathophysiological heterogeneity.
Characterization of SURF and BRISK Interest Point Distribution for Distributed Feature Extraction in Visual Sensor Networks

We study the statistical characteristics of SURF and BRISK interest points and descriptors, with the aim of supporting the design of distributed processing across sensor nodes in a resource -constrained visual sensor network (VSN). Our results show high variability in the density, the spatial distribution , and the octave layer distribution of the interest points. The high variability implies that balancing the processing load among the sensor nodes is a very challenging task, and obtaining a priori information is essential, e.g., through prediction . Our results show that if a priori information is available about the images, then Top- M interest point selection, limited , octave-based processing at the camera node, together with area-based interest point detection and extraction at the processing nodes, can balance the processing load and limit the transmission cost in the network . Complete interest point detection at the camera node with optimized descriptor extraction delegation to the processing nodes in turn can further decrease the transmission load and allow a better balance of the processing load among the network nodes.
HIV/AIDS Clinical Manifestations and their Implication for Patient Clinical Staging in Resource Limited Settings in Tanzania

Background: Tanzania HIV/AIDS management follows WHO clinical staging which requires CD4 counts as complement. Lacking CD4 counts facilities in rural health facilities remains a challenge. Simplified and sensitive clinical staging based on local clinical patterns is useful to ensure effective care without CD4 counts. Objectives: To assess whether local HIV clinical manifestations can be used to guide HIV management in settings with limited access to CD4 counts in Tanzania. Methods: A Cross-sectional study conducted at Tumbi and Chalinze health facilities documented clinical manifestations and CD4 counts in 360 HIV/AIDS patients. Simplified management groups comprised of severe and moderate disease were formed based on clinical manifestations and CD4 counts results. Symptoms with high frequency were used to predict severe disease. Results: A Weight loss (48.3%) and chronic cough (40.8 %) were the most reported manifestations in the study population. More than 50% of patients presented with CD4≤200. Most symptoms were found to be highly sensitive (71% to 93%) in predicting severe immunosuppression using CD4<200 cut-off point as a ‘Gold standard’. Chronic diarrhoea presented in 10.6%, and predicted well severe immunosuppression either alone (OR 1.95, 95%CI, 0.95-4.22) or in combination (OR 4.21, 95%CI 0.92-19.33) with other symptoms. Basing strictly on WHO clinical staging 30.8% of patients were detected to be severely immunosuppressed (Stage 4). While using our proposed management categories of severe and moderate immunosuppression 70% of patients were put into the severe immunosuppression group, consistent with CD4 cut-off count of≤350. Conclusions: HIV/AIDS clinics managing large cohorts should develop validated site specific guidelines based on local experiences. Simplified guidelines are useful for resource constrained settings without CD4 counting facilities.
Spirulina Promotes Stem Cell Genesis and Protects against LPS Induced Declines in Neural Stem Cell Proliferation

Adult stem cells are present in many tissues including, skin, muscle, adipose, bone marrow, and in the brain. Neuroinflammation has been shown to be a potent negative regulator of stem cell and progenitor cell proliferation in the neurogenic regions of the brain. Recently we demonstrated that decreasing a key neuroinflammatory cytokine IL-1β in the hippocampus of aged rats reversed the age-related cognitive decline and increased neurogenesis in the age rats. We also have found that nutraceuticals have the potential to reduce neuroinflammation, and decrease oxidative stress. The objectives of this study were to determine if spirulina could protect the proliferative potential of hippocampal neural progenitor cells from an acute systemic inflammatory insult of lipopolysaccharide (LPS). To this end, young rats were fed for 30 days a control diet or a diet supplemented with 0.1% spirulina. On day 28 the rats were given a single i.p. injection of LPS (1 mg/kg). The following day the rats were injected with BrdU (50 mg/kg b.i.d. i.p.) and were sacrificed 24 hours after the first injection of BrdU. Quantification of the BrdU positive cells in the subgranular zone of the dentate gyrus demonstrated a decrease in proliferation of the stem/progenitor cells in the hippocampus as a result of the LPS insult. Furthermore, the diet supplemented with spirulina was able to negate the LPS induced decrease in stem/progenitor cell proliferation. In a second set of studies we examined the effects of spirulina either alone or in combination with a proprietary formulation (NT-020) of blueberry, green tea, vitamin D3 and carnosine on the function of bone marrow and CD34+ cells in vitro. Spirulina had small effects on its own and more than additive effects in combination with NT-020 to promote mitochondrial respiration and/or proliferation of these cells in culture. When examined on neural stem cells in culture spirulina increased proliferation at baseline and protected against the negative influence of TNFα to reduce neural stem cell proliferation. These results support the hypothesis that a diet enriched with spirulina and other nutraceuticals may help protect the stem/progenitor cells from insults.
Real interference alignment for the MIMO multiple access wiretap channel

We consider a two-user multiple-input multiple-output (MIMO) multiple access wiretap channel with N antennas at each transmitter, N antennas at the legitimate receiver, and K antennas at the eavesdropper. We determine the optimal sum secure degrees of freedom (s.d.o.f.) when the channel gains are fixed for the duration of the communication. We provide optimal achievable schemes based on a combination of Gaussian signaling and real interference alignment for all regimes of N and K.
Natural killer cells do not play a dominant role in CD4+ subset differentiation in Candida albicans-infected mice

The effects of in vivo administration of monoclonal antibodies against NK-1.1-bearing cells on the early production of gamma interferon (IFN-gamma) in vitro and development of Th1-associated immunity were studied in mice infected with a live vaccine strain of Candida albicans. At 1 and 4 days postinfection, natural killer (NK) cell-enriched fractions from the spleens of antibody-treated mice displayed a dramatic reduction in 5E6+ lymphocytes and negligible anti-YAC-1 cytotoxic activity in vitro. Nevertheless, the frequency of IFN-gamma-producing cells in those fractions was reduced by less than half, on average, by anti-NK-1.1 treatment in vivo. In addition, the antibody-treated and infected mice demonstrated unchanged T helper cell responses, as measured by yeast-specific footpad reactions, resistance to reinfection, occurrence of antibodies of different isotypes, and production in vitro of interleukin-2 (IL-2), IFN-gamma, IL-4, and IL-10 by CD4+ cells. Therefore, although NK cells may contribute to early IFN-gamma production in Candida-vaccinated mice, these cells apparently do not play a dominant role in the qualitative development of yeast-specific T helper responses.
Characterization of Bacteroides forsythus isolates

Fifteen Bacteroides forsythus strains freshly isolated from patients with periodontitis were used together with three collection strains and one type strain for characterization of growth on various media; determination of enzymatic profiles, antibiotic susceptibility profiles, 16S rRNA ribotypes, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) outer membrane protein profiles, and pathogenicity; and gas chromatography analysis by using a wound chamber model in rabbits. All strains were stimulated by N-acetylmuramic acid, while one strain needed a further supplement such as yeast extract for optimal growth. All strains showed trypsin-like activity. While 10 different ribotypes were found, the SDS-PAGE profiles revealed similar patterns for all strains. All strains were sensitive to penicillin G (MICs, <0.5 microg/ml), ampicillin (MICs, <1.0 microg/ml), amoxicillin (MICs, <0.38 microg/ml), metronidazole (MICs, <0.005 microg/ml), tetracycline (MICs, <0.19 microg/ml), doxycycline (MICs, 0.05 microg/ml), erythromycin (MICs, <0.4 microg/ml), and clindamycin (MICs, <0.016 microg/ml), while they were less sensitive to ciprofloxacin (MICs, <4 microg/ml). B. forsythus did not cause abscess formation by monoinoculation. B. forsythus coinoculated with Fusobacterium nucleatum ATCC 10953 caused abscess formation in 75% of rabbits, while it caused abscess formation in 100% of rabbits when it was coinoculated with Porphyromonas gingivalis FDC 381. In the case of the latter combination, four of six rabbits died of sepsis after 6 to 7 days, and P. gingivalis and B. forsythus were recovered from the heart blood at a proportion of 10:1. B. forsythus strains were highly virulent and invasive in combination with P. gingivalis.
Rapid differentiation of streptococci isolated from cows with mastitis

The demonstration of some species-specific streptococcal enzymes with 4-methylumbelliferyl-conjugated beta-D-glucuronide, N-acetyl-beta-D-glucosaminide, and beta-D-mannoside and agglutination with the lectin from Dolichos biflorus allowed rapid tentative identification of streptococci isolated from cows with mastitis.
Effect of genistein on expression of pancreatic SIRT1, inflammatory cytokines and histological changes in ovariectomized diabetic rat

Objective(s): Genistein is reported to have anti-diabetic and anti-inflammatory functions, in particular, direct effects on β-cell proliferation and insulin secretion. In this study, we investigated the anti-inflammatory effect of genistein on the pancreatic β-cells in ovariectomized diabetic rat. Materials and Methods: Forty female rats were divided into four groups: sham, bilateral ovariectomy (OVX), OVX.D (OVX+diabetes) and OVX.D. G (OVX.D+genistein). After bilateral ovariectomy, rats in the diabetic groups were fed high-fat diet (HFD), ad libitum for 4 weeks, and then a low dose of streptozotocin (STZ) (30 mg/kg) injected intraperitoneally. Genistein (1 mg/kg/day; SC) was administrated for 8 weeks. At the end of 8 weeks, pancreas tissue was removed and used for western blotting and Hematoxylin-Eosin staining. Results: Treatment with genistein declined inflammation and tissue injury, and this decline was correlated with the expression of SIRT1. OVX and OVX.D significantly increased Nf-κB and IL-1β expression and decreased SIRT1 levels compared to sham group (P<0.05). Significant reduction of Nf-κB and IL-1β, and increasing of SIRT1 were observed during genistein treatment (P<0.05). Conclusion: Estrogen deficiency alone or with HFD increased pancreatic inflammation. However, subcutaneous administration of gtenistein prevented from these inflammatory changes in the pancreas of a surgery animal model of ovariectomy with or without diabetes. Our results support the potential preventing effect of genistein from pancreatic injury.
Flow Corrosion Behavior of Austenitic Stainless Steels UNS S30403 and UNS S31603

Abstract Metastable pitting behavior for two grades of austenitic stainless steels, Type 304L (UNS S30403) and Type 316L (UNS S31603), has been investigated for a range of controlled hydrodynamic conditions: static, laminar, and turbulent (Reynolds numbers of 1,000, 2,000, 3,000, 10,000, 50,000, and 75,000). Metastable pitting was evident under all flow regimes. Stable pit growth was most evident for laminar flow conditions immediately before the transition to turbulent flow and during the approach to the so-called “critical velocity” (∼1.5 m/s) at Reynolds numbers of approximately 2,000 and 50,000, respectively. The corrosion behavior of the two austenitic stainless steels has been compared with a superduplex stainless steel, UNS S32760, exposed to the same hydrodynamic conditions.
An Investigation of Multi-Agent Planning in CLP

This paper explores the use of Constraint Logic Programming (CLP) as a platform for experimenting with planning problems in the presence of multiple interacting agents. The paper develops a novel constraint-based action language, B MAP, that enables the declarative description of large classes of multi-agent and multi-valued domains. B MAP supports several complex features, including combined effects of concurrent and interacting actions, concurrency control, and delayed effects. The paper presents a mapping of B MAP theories to CLP and it demonstrates the effectiveness of an implementation in SICStus Prolog on several benchmark problems. The effort is an evolution of previous research on using CLP for single-agent domains, demonstrating the flexibility of CLP technology to handle the more complex issues of multi-agency and concurrency.
Improving Erosion Resistance of Polymer Reinforced Composites

This article reports on solid particle erosion tests performed on glass fiber reinforced polyphenylene sulphide (PPS) for use on an all-composite ice-protection system for aircraft structures. The idea was introduced to use a metal mesh as a convenient way of toughening the surface layer thereby increasing erosion resistance. The erosion rates of the composites were evaluated at different impingement angles and with different erodent particle size. An aircraft-grade aluminium sheet was also used to provide a comparison for the composites. It was found that the erosion rate of the composites drastically reduced upon the introduction of an impregnated mesh onto the surface of the baseline glass/PPS laminate, to values similar to that of the aluminium sheet. In addition, the erosion rate was found to be dependent not only on the volume fraction of the matrix on the surface, but also the properties of the metal used for the mesh. The solution to use a metal mesh was shown to have multifunctional uses for composite based ice-protection system.
Thyroid Hormone-disrupting Effects and the Amphibian Metamorphosis Assay

There are continued concerns about endocrine-disrupting chemical effects, and appropriate vertebrate models for assessment of risk are a high priority. Frog tadpoles are very sensitive to environmental substances because of their habitat and the complex processes of metamorphosis regulated by the endocrine system, mainly thyroid hormones. During metamorphosis, marked alteration in hormonal factors occurs, as well as dramatic structural and functional changes in larval tissues. There are a variety of mechanisms determining thyroid hormone balance or disruption directly or indirectly. Direct-acting agents can cause changes in thyroxine synthesis and/or secretion in thyroid through effects on peroxidases, thyroidal iodide uptake, deiodinase, and proteolysis. At the same time, indirect action may result from biochemical processes such as sulfation, deiodination and glucuronidation. Because their potential to disrupt thyroid hormones has been identified as an important consideration for the regulation of chemicals, the OECD and the EPA have each established guidelines that make use of larval African clawed frogs (Xenopus laevis) and frog metamorphosis for screening and testing of potential endocrine disrupters. The guidelines are based on evaluation of alteration in the hypothalamic-pituitary-thyroid axis. One of the primary endpoints is thyroid gland histopathology. Others are mortality, developmental stage, hind limb length, snout-vent length and wet body weight. Regarding histopathological features, the guidelines include core criteria and additional qualitative parameters along with grading. Taking into account the difficulties in evaluating amphibian thyroid glands, which change continuously throughout metamorphosis, histopathological examination has been shown to be a very sensitive approach.
Triplet repeat RNA structure and its role as pathogenic agent and therapeutic target

This review presents detailed information about the structure of triplet repeat RNA and addresses the simple sequence repeats of normal and expanded lengths in the context of the physiological and pathogenic roles played in human cells. First, we discuss the occurrence and frequency of various trinucleotide repeats in transcripts and classify them according to the propensity to form RNA structures of different architectures and stabilities. We show that repeats capable of forming hairpin structures are overrepresented in exons, which implies that they may have important functions. We further describe long triplet repeat RNA as a pathogenic agent by presenting human neurological diseases caused by triplet repeat expansions in which mutant RNA gains a toxic function. Prominent examples of these diseases include myotonic dystrophy type 1 and fragile X-associated tremor ataxia syndrome, which are triggered by mutant CUG and CGG repeats, respectively. In addition, we discuss RNA-mediated pathogenesis in polyglutamine disorders such as Huntington's disease and spinocerebellar ataxia type 3, in which expanded CAG repeats may act as an auxiliary toxic agent. Finally, triplet repeat RNA is presented as a therapeutic target. We describe various concepts and approaches aimed at the selective inhibition of mutant transcript activity in experimental therapies developed for repeat-associated diseases.
Growing Grapes in Your Computer to Defend Against Malware

Behavior-based detection is promising to resolve the pressing security problem of malware. However, the great challenge lies in how to detect malware in a both accurate and light-weight manner. In this paper, we propose a novel behavior-based detection method, named growing grapes, aiming to enable accurate online detection. It consists of a clustering engine and detection engine. The clustering engine groups the objects, e.g., processes and files, of a suspicious program together into a cluster, just like growing grapes. The detection engine recognizes the cluster as malicious if the behaviors of the cluster match a predefined behavior template formed by a set of discrete behaviors. The approach is accurate since it identifies a malware based on multiple behaviors and the source of the processes requesting the behaviors. The approach is also light-weight as it uses OS-level information flows instead of data flows that generally impose significant performance impact on the system. To further improve the performance, a novel method of organizing the behavior template and template database is proposed, which not only makes the template matching process very quick, but also makes the storage space small and fixed. Furthermore, the detection accuracy and performance are optimized to the best degree using a combinatorial optimization algorithm, which properly selects and combines multiple behaviors to form a template for malware detection. Finally, the approach novelly identifies malicious OS objects in a cluster fashion rather than one by one as done in traditional methods, which help users to thoroughly eliminate the changes of a malware without malware family knowledge. Compared with commercial antimalware tools, extensive experiments show that our approach can detect new malware samples with higher detection rate and lower false positive rate while imposing low overhead on the system.
Radical cystectomy with orthotopic neobladder for invasive bladder cancer: a critical analysis of long term oncological, functional and quality of life results.

PURPOSE Analyze current knowledge and practice regarding tumor-related cystectomy with subsequent orthotopic neobladder both in male and female patients. DESIGN, SETTING, AND PARTICIPANTS Evaluate literature predominantly from the last decade dealing with long-term experience in large numbers of patients with an orthotopic neobladder following cystectomy. Oncological outcome specific to an orthotopic neobladder, functional aspects such as urinary continence, renal function, sexual activity and other quality of life issues are elucidated. RESULTS Local pelvic recurrences after urothelial bladder cancer occur in 7-12%. Urethral second primary tumors in male and female patients in contemporary series with bladder substitution are 4-6% and 1.4 o 4%, respectively. Upper tract recurrences vary between 2.4-17%. Complications regarding the upper urinary tract have dramatically diminished due to simplified forms of upper tract protection as well as a more refined technique of ureterointestinal anastomosis. Depending on the technique ureteroileal stenosis was lately reported to lie between 2.7 to 3.8%. Renal function remained stable in 96% after a mean follow-up of up to 5 years. CONCLUSION Radical cystectomy in carefully selected patients has stood the test of time by providing adequate long-term survival and low local recurrence rates. Orthotopic bladder substitution does not compromise oncological outcome, yields excellent functional results, is cost effective compared to other types of urinary diversion, may improve quality of life and should therefore be the diversion of choice both in men and women. Chronological age is generally not a contraindication for cystectomy, but for orthotopic urinary diversion, tumor extent, functional pelvic floor deficits and general life expectancy are limiting factors.