Miracle-dz/UniFAR-FTUs huggingface dataset:
A Cysteine Zipper Stabilizes a Pre-Fusion F Glycoprotein Vaccine for Respiratory Syncytial Virus

Recombinant subunit vaccines should contain minimal non-pathogen motifs to reduce potential off-target reactivity. We recently developed a vaccine antigen against respiratory syncytial virus (RSV), which comprised the fusion (F) glycoprotein stabilized in its pre-fusion trimeric conformation by “DS-Cav1” mutations and by an appended C-terminal trimerization motif or “foldon” from T4-bacteriophage fibritin. Here we investigate the creation of a cysteine zipper to allow for the removal of the phage foldon, while maintaining the immunogenicity of the parent DS-Cav1+foldon antigen. Constructs without foldon yielded RSV F monomers, and enzymatic removal of the phage foldon from pre-fusion F trimers resulted in their dissociation into monomers. Because the native C terminus of the pre-fusion RSV F ectodomain encompasses a viral trimeric coiled-coil, we explored whether introduction of cysteine residues capable of forming inter-protomer disulfides might allow for stable trimers. Structural modeling indicated the introduced cysteines to form disulfide “rings”, with each ring comprising a different set of inward facing residues of the coiled-coil. Three sets of rings could be placed within the native RSV F coiled-coil, and additional rings could be added by duplicating portions of the coiled-coil. High levels of neutralizing activity in mice, equivalent to that of the parent DS-Cav1+foldon antigen, were elicited by a 4-ring stabilized RSV F trimer with no foldon. Structure-based alteration of a viral coiled-coil to create a cysteine zipper thus allows a phage trimerization motif to be removed from a candidate vaccine antigen.
The signer as an embodied mirror neuron system: neural mechanisms underlying sign language and action

Introduction “Mirror” neurons are found in area F5 of the monkey brain, and they fire both when a monkey grasps an object and when the monkey observes another individual grasping the object (e.g., Rizzolatti et al ., 1996; see Arbib, Chapter 1, this volume, for further discussion). Mirror neurons have also been found in the rostral part of the monkey inferior parietal lobule (Gallese et al ., 2002). Like mirror neurons, signers must associate the visually perceived manual actions of another signer with self-generated actions of the same form. Sign language comprehension and production requires a direct coupling between action observation and action execution. However, unlike mirror neurons for hand movements recorded in monkey, signing is not tied to object manipulation. Mirror neurons for grasping in monkey fire only when an object is present or understood to be present and do not fire when just the grasping movement is presented (Umilta et al ., 2001). Furthermore, unlike grasping and reaching movements, sign articulations are structured within a phonological system of contrasts. The hand configuration for a sign is determined by a phonological specification stored in the lexicon, not by the properties of an object to be grasped. These facts have interesting implications for the evolution of language and for the neural systems that underlie sign language and action. The fact that sign language exhibits form-based patterning of meaningless elements (i.e., phonology) distinguishes signs from actions, even when the two appear quite similar on the surface; for example, the American Sign Language (ASL) sign TO-HAMMER resembles the act of hammering.
Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

Background Preeclampsia (PE) is a pregnancy-specific syndrome manifested by on-set of hypertension and proteinuria after 20 weeks of gestation. Abnormal placenta development has been generally accepted as initial cause of the disorder. Recently, miR-195 was found to be down-regulated in preeclamptic placentas compared with normal pregnant ones, indicating possible association of this small molecule with placental pathology of preeclampsia. By far the function of miR-195 in the development of placenta remains unknown. Methodology/Principal Findings Bioinformatic assay predicted ActRIIA as one of the targets for miR-195. By using Real-time PCR, Western blotting and Dual Luciferase Assay, we validated that ActRIIA was the direct target of miR-195 in human trophoblast cells. Transwell insert invasion assay showed that miR-195 could promote cell invasion in trophoblast cell line, HTR8/SVneo cells, and the effect could be abrogated by overexpressed ActRIIA. In preeclamptic placenta tissues, pri-miR-195 and mature miR-195 expressions were down-regulated, whereas ActRIIA level appeared to be increased when compared with that in gestational-week-matched normal placentas. Conclusions/Significance This is the first report on the function of miR-195 in human placental trophoblast cells which reveals an invasion-promoting effect of the small RNA via repressing ActRIIA. Aberrant expression of miR-195 may contribute to the occurrence of preeclampsia through interfering with Activin/Nodal signaling mediated by ActRIIA in human placenta.
Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs

Continuous imaging of live tissues provides clear temporal sequence of biological events. The Drosophila imaginal discs have been popular experimental subjects for the study of a wide variety of biological phenomena, but long term culture that allows normal development has not been satisfactory. Here we report a culture method that can sustain normal development for 18 hours and allows live imaging. The method is validated in multiple discs and for cell proliferation, differentiation and migration. However, it does not support disc growth and cannot support cell proliferation for more than 7 to 12 hr. We monitored the cellular behavior of retinal basal glia in the developing eye disc and found that distinct glia type has distinct properties of proliferation and migration. The live imaging provided direct proof that wrapping glia differentiated from existing glia after migrating to the anterior front, and unexpectedly found that they undergo endoreplication before wrapping axons, and their nuclei migrate up and down along the axons. UV-induced specific labeling of a single carpet glia also showed that the two carpet glia membrane do not overlap and suggests a tiling or repulsion mechanism between the two cells. These findings demonstrated the usefulness of an ex vivo culture method and live imaging.
Religion as a Human Right and a Security Threat—Investigating Young Adults’ Experiences of Religion in Finland

The emergence of religiously motivated terrorist attacks and the increasing xenophobia expressed in Europe concern religions in many ways. Questions related to religion also lie at the core of educational aims and practices used to create national cohesion and understanding about different types of values and worldviews. However, despite the topicality of the issue, we have little knowledge about the ways in which young adults experience religions in a secular state. In order to contribute to the discussion regarding the relationships between religion, nationality, security, and education, this study focuses on investigating how politically active young adults experience the role of religions in Finnish society. The qualitative data of this study were collected from young adults (18–30-year-olds) through an online questionnaire distributed through political youth organisations. The content analysis of the responses (altogether 250 respondents) identified five main orientations towards religions. The findings highlight the importance of providing young people with education about different faiths and worldviews for reducing prejudices, especially those related to Islam. The findings also highlight the need to address in education and society the possible but not as self-evident relationship between violence and religion, and to do this more explicitly than is currently done.
A Survey of Large Scale Data Management Approaches in Cloud Environments

In the last two decades, the continuous increase of computational power has produced an overwhelming flow of data. Moreover, the recent advances in Web technology has made it easy for any user to provide and consume content of any form. This has called for a paradigm shift in the computing architecture and large scale data processing mechanisms. Cloud computing is associated with a new paradigm for the provision of computing infrastructure. This paradigm shifts the location of this infrastructure to the network to reduce the costs associated with the management of hardware and software resources. This paper gives a comprehensive survey of numerous approaches and mechanisms of deploying data-intensive applications in the cloud which are gaining a lot of momentum in both research and industrial communities. We analyze the various design decisions of each approach and its suitability to support certain classes of applications and end-users. A discussion of some open issues and future challenges pertaining to scalability, consistency, economical processing of large scale data on the cloud is provided. We highlight the characteristics of the best candidate classes of applications that can be deployed in the cloud.
Central Vs. Peripheral Manifestations of Neuromuscular Force in Persons with Parkinsonʼs Disease

Hammond, Kelley Grace. M.S. The University of Memphis. May 2010. Central versus peripheral manifestations of neuromuscular force production in persons with Parkinson’s disease. Major Professor: Brian K. Schilling, Ph.D. Bradykinesia and reduced neuromuscular force exist in Parkinson’s disease (PD). Percutaneous electrical stimulation (PES) has been used to evaluate central versus peripheral manifestations of neuromuscular strength in healthy, aging, and athletic populations, but this method has not previously been used in PD. This pilot study used PES of the quadriceps femoris to identify central and peripheral activation in persons with PD (n = 7) and neurologically healthy controls (n = 6). Maximal voluntary rate of force development (PD = 2544N/s ± 1183, control = 4599N/s ± 1077; P = 0.008) and the rate of force development ratio (RFDR; PD = 0.45 ± 0.15, control = 0.80 ± 0.20; P = 0.004) were significantly (P < 0.05) lower in the PD group compared to controls. No other significant differences were found between groups. This study was the first to quantify the central deficits caused by PD which bring about reduced neuromuscular rate of force development.
How WEIRD are positive psychology interventions? A bibliometric analysis of randomized controlled trials on the science of well-being

ABSTRACT The past two decades have witnessed a rapid rise in well-being research, and a profusion of empirical studies on positive psychology interventions (PPIs). This bibliometric analysis quantifies the extent to which rigorous research on PPIs that employ randomized controlled trials (RCTs) reaches beyond Western Educated Industrialized Rich Democratic (WEIRD) populations. A search was conducted through databases including PubMed, PsycINFO, and Scopus for studies from 1998 to 2017. In total, we found 187 full-text articles that included 188 RCTs from 24 countries. We found that RCTs on the efficacy of PPIs are still predominately conducted in western countries, which accounted for 78.2% of the studies. All these countries are highly industrialized and democratic, and study populations are often highly educated and have a high income. However, there has been a strong and steady increase in publications from non-Western countries since 2012, indicating a trend towards globalization of positive psychology research.
A Comparative Study of the Sodium Content and Calories from Sugar in Toddler Foods Sold in Low- and High-Income New York City Supermarkets

Information from the nutrition facts labels of toddler foods marketed in low- and high-income New York City zip codes were analyzed for sodium content, the proportion of sugar-derived calories, and presence of sugar and/or high-fructose corn syrup as an added sweetener in the list of ingredients. Among the 272 toddler foods analyzed, more than a quarter were high in sodium, over one-third derived at least 20% their calories from sugar, and more than 41% of the foods had sugar and/or high-fructose corn syrup listed among the first five ingredients. The proportion of foods with such nutritional characteristics did not significantly differ between the low- and high-income neighborhood supermarkets. Median sodium content was highest among “side dishes” and “meals. ” The proportion of calories derived from sugar was found to be highest among “snacks and yogurt blends” in both low- and high-income neighborhoods and “breakfast foods and cereals” in low-income neighborhoods. When compared to high-income neighborhoods, more than three times the proportion of total calories in “breakfast foods and cereals” sold in low-income neighborhoods were derived from sugar. Since taste preferences established during childhood can have long-lasting influence on dietary habits, it is imperative to limit the promotion of toddler foods that are high in sodium and sugar as well as educate parents to make nutritionally sound decisions at the point of purchase.
Pre-diagnostic Clinical Presentations and Medical History Prior to the Diagnosis of Inflammatory Bowel Disease in Children

Purpose The clinical presentations of inflammatory bowel disease (IBD) prior to diagnosis are so diverse or vague that many of them waste time before final diagnosis. This study was undertaken to know the medical history of the pediatric patients until the final diagnosis could be reached. Methods The medical records of all pediatric patients who were diagnosed with IBD (Crohn's disease [CD] in 14 children, ulcerative colitis [UC] in 17) during the last 13 years were reviewed. We investigated the length of the diagnostic time lag, chief clinical presentation, and any useful laboratory predictor among the routinely performed examinations. Indeterminate colitis was not included. Results The mean ages of children at the final diagnosis was similar in both diseases. As for the pre-clinical past history of bowel symptoms in CD patients, 5 were previously healthy, 9 had had 1-3 gastrointestinal (GI) symptoms, weight loss, bloody stool, anemia and rectal prolapse. With UC, 9 were previously healthy, 8 had had 1-3 GI symptoms, bloody stool, anorexia. The average diagnostic time lag with CD was 3.36 months, and with UC 2.2 months. Body mass index (BMI) and the initial basic laboratory data (white blood cell, hemoglobin, mean corpuscular volume, serum albumin, and serum total protein) were lower in CD, statistically significant only in BMI. Conclusion IBD shows diverse clinical symptoms before its classical features, making the patients waste time until diagnosis. It is important to concern possibility of IBD even in the mildly sick children who do not show the characteristic symptoms of IBD.
There is no degree invariant half-jump

We prove that there is no degree invariant solution to Post’s problem that always gives an intermediate degree. In fact, assuming definable determinacy, if W is any definable operator on degrees such that a <W (a) < a on a cone then W is low2 or high2 on a cone of degrees, i. e. there is a degree c such that W (a) = a′′ for every a ≥ c or W (a) = a′′′ for every a ≥ c. ∗Research partially supported by the U.S. ARO through ACSyAM at the Mathematical Sciences Institute of Cornell University Contract DAAL03-91-C-0027, the IGC of Victoria University and the Marsden Fund for Basic Science under grant VIC-509. §Research partially supported by NSF Grant DMS-9503503 and the U.S. ARO through ACSyAM at the Mathematical Sciences Institute of Cornell University Contract DAAL0391-C-0027.
Yield Variability and Agricultural Trade

We examine how changes in yield variability affect the welfare of cereal grain and oilseed buyers and producers around the world. We simulate trade patterns and welfare for 21 countries with a Ricardian trade model that incorporates bilateral trade costs and crop yield distributions. The model shows that world trade volumes would need to increase substantially if crop yield variability were to rise. Net welfare effects, however, are moderate so long as countries do not resort to policies that inhibit trade, such as export restrictions or measures to promote self-sufficiency in crops. Low-income countries suffer the most from increases in yield variability, due to higher bilateral trade costs and lower-than-average productivity.
Theoretical and Experimental Evaluation of Superstrate Effect on Rectangular Patch Resonator Parameters

In this paper, modeling and experimentation of a Rectangular Patch Resonator (RPR) covered with a dielectric superstrate are investigated. The RPR criteria are established theoretically and experimentally, to be used in future prospects as an electromagnetic (EM) sensor for the characterization of superstrates. The theoretical model is based on the moment method (MoM) via Galerkin's approach, in which three types of basis and testing functions are used. These functions as well as the spectral dyadic Green function are efficiently implanted with compact structured Fortran 90 codes. The EM commercial HFSS and CST Microwave Studio softwares are used to simulate the proposed RPR prototypes. The accuracy of the obtained results is assessed using four prototypes of RPRs operating around 6 GHz, taking into account only the resonant frequency of the fundamental dominant mode. The theoretical model is compared to simulation and measurement results, and very good agreements are observed.
Enhancement of students’ creative thinking skills on mixture separation topic using project based student worksheet

The aim of this study is to describe the effectiveness of project based student worksheet in improving students' creative thinking skills. The research method is using quasi experiment with the matching only pre-test post-test control group design. The population in this research is all students of class VII SMP N 2 Belitang Madang Raya with class VII1 as control class and class VII4 as experiment class. The sample of this research is obtaining by purposive sampling technique. The effectiveness of project based student worksheet is based on significant post-test differences between the control class and the experiment class as well as the effect size. The results show that the using of project based student worksheet is effective in improving students' creative thinking skills on mixture separation topic.
Organizational identity in acculturation in cross-border acquisitions: Implications for HRD practitioners in global M&A

ABSTRACT The purpose of this study is to explore the role of organizational identity in acculturation, or organizational culture development, between the acquired and the acquiring organizations during a cross-border acquisition. The goal is to identify how long-term and short-term employees identify with the acquired organization, and the implications for acculturation for both groups. It is also to help identify implications for Human Resource Development Practitioners in this process. The long-term employees (more than seven years) of the acquired organization identified more strongly with the organization than the short-term (three or less years) employees in this study. The short-term employees seemed to welcome the acquiring Multinational Company (MNC), and readily adapted to their multicultural identity, while long-term employees were more concerned with maintaining the reputation of the original organization. The study provides Human Resource Development (HRD) practitioners with a better understanding of how and why organizational identity is an important aspect to consider in the acculturation process in cross-border mergers and acquisitions. It also provides insight into how to work with organizational identity with acquired organizations in integration and adaptation to the new organization by understanding the differences in organizational identity of long-term and short-term employees in India.
ON THE IRREDUCIBILITY, SELF-DUALITY, AND NON-HOMOGENEITY OF COMPLETELY POSITIVE CONES

Abstract. For a closed cone Cin R n , the completely positive cone of Cis the convex cone K C in S n generated by fuu T : u2Cg. Such a cone arises, for example, in the conic LP reformula-tion of a nonconvex quadratic minimization problem over an arbitrary set with linear and binaryconstraints. Motivated by the useful and desirable properties of the nonnegative orthant and thepositive semide nite cone (and more generally of symmetric cones in Euclidean Jordan algebras),this paper investigates when (or whether) K C can be irreducible, self-dual, or homogeneous.Key words. Copositive cones, Completely positive cones, Self-dual, Irreducible cone, Homoge-neous cone.AMS subject classi cations. 15B48, 90C20. 1. Introduction. Given a closed cone Cin R n that is not necessarily convex,we consider two related cones in the space S n of all n nreal symmetric matrices:The completely positive cone of Cde ned byK C :=nXuu T : u2Co(1.1) ;where the sum denotes a nite sum, and the copositive cone of Cgiven by(1.2) E
Linear dynamical models in speech synthesis

Hidden Markov models (HMMs) are becoming the dominant approach for text-to-speech synthesis (TTS). HMMs provide an attractive acoustic modeling scheme which has been exhaustively investigated and developed for many years. Modern HMM-based speech synthesizers have approached the quality of the best state-of-the-art unit selection systems. However, we believe that statistical parametric speech synthesis has not reached its potential, since HMMs are limited by several assumptions which do not apply to the properties of speech. We, therefore, propose in this paper to use Linear Dynamical Models (LDMs) instead of HMMs. LDMs can better model the dynamics of speech and can produce a naturally smoother trajectory of the synthesized speech. We perform a series of experiments using different system configurations to check on the performance of LDMs for speech synthesis. We show that LDM-based synthesizers can outperform HMM-based ones in terms of cepstral distance and are a very promising acoustic modeling alternative for statistical parametric TTS.
Towards an Optimal Teacher Salary Schedule: Designing Base Salary to Attract and Retain Effective Teachers

This study presents the literature’s first estimates of the effect of teacher pay on the distribution of experience among teachers hired by public school districts. Overall, a 1% increase in base salary for teachers of a particular experience level increases the proportion of the targeted teachers hired by 0.04–0.08 percentage points. Pay increases have the largest effect on hire rates among teachers with 2–3 years of experience and the effect diminishes with experience. I show that higher teacher salaries provide a dual benefit of retaining and attracting a more effective distribution of teachers. Districts may also improve student achievement growth at no cost by reshaping their salary schedules so that they are increasing and concave in teacher experience.
Modeling of an Optical Sensor Based on Whispering Gallery Modes (WGMs) on the Surface Guiding Layer of Glass Filaments

A ring-resonator-based refractive index sensor is proposed in this paper. Glass filaments with surface guiding layers created by ion exchange are crossed with a fiber taper to act as a ring resonator sensor. Theoretical simulation of the sensor response is proposed, and optimization of structural parameters including thickness and refractive index of the surface guiding layer and the diameter of the ring resonator for higher sensitivity is investigated. Results show that a detection limit of a variation of ∼10-5RIU can be reached. Due to its simple fabrication and easy manipulation as well as good sensing performance, we believe such a micro-cavity sensor will find potential applications in high sensitivity optical sensing.