Miracle-dz/UniFAR-FTUs huggingface dataset:
Methylglyoxal-Mediated Stress Correlates with High Metabolic Activity and Promotes Tumor Growth in Colorectal Cancer

Cancer cells generally rely on aerobic glycolysis as a major source of energy. Methylglyoxal (MG), a dicarbonyl compound that is produced as a side product during glycolysis, is highly reactive and induces the formation of advanced glycation end-products that are implicated in several pathologies including cancer. All mammalian cells have an enzymatic defense against MG composed by glyoxalases GLO1 and GLO2 that converts MG to d-lactate. Colorectal cancer (CRC) is one of the most frequently occurring cancers with high morbidity and mortality. In this study, we used immunohistochemistry to examine the level of MG protein adducts, in a series of 102 CRC human tumors divided into four clinical stages. We consistently detected a high level of MG adducts and low GLO1 activity in high stage tumors compared to low stage ones suggesting a pro-tumor role for dicarbonyl stress. Accordingly, GLO1 depletion in CRC cells promoted tumor growth in vivo that was efficiently reversed using carnosine, a potent MG scavenger. Our study represents the first demonstration that MG adducts accumulation is a consistent feature of high stage CRC tumors. Our data point to MG production and detoxification levels as an important molecular link between exacerbated glycolytic activity and CRC progression.
Accent and Discourse Context: Assigning Pitch Accent in Synthetic Speech

Identifying the regularities underlying speaker decisions to emphasize or de-emphasize an item intonationally has long been the subject of speculation and controversy. This paper describes a study of accent assignment based upon the analysis of natural recorded (read) speech. Results are being incorporated in NewSpeak, an interface to the Bell Laboratories Text-to-Speech System, which varies intonational features based upon syntactic structure and higher-level discourse information inferred from unrestricted text, in order to generate more natural synthetic speech. Implications of the work for the evaluation of discourse models, for automatic labeling of prosodic features, and for speech synthesis are discussed.
Galectin-1 Accumulation in the Ovary Carcinoma Peritumoral Stroma Is Induced by Ovary Carcinoma Cells and Affects Both Cancer Cell Proliferation and Adhesion to Laminin-1 and Fibronectin

Galectin-1 (gal-1) is a 14-kDa laminin-binding galectin involved in several biologic events including regulation of cancer cell proliferation and adhesion to the matrix. In this study, we examined gal-1 expression in 30 human epithelial ovary carcinoma samples by Western and Northern blotting and by immunohistochemistry. Gal-1 mRNA levels were increased in more than 95% of the examined ovary carcinoma samples, compared with a wedge resection of a normal ovary. Immunohistochemical analysis of the samples demonstrated gal-1 expression in cancer epithelial cells from 17 of 30 samples, with a cytoplasmic pattern. Gal-1 immunostaining was significantly increased in the stroma associated with carcinoma cells compared with the normal, noninvaded stroma (p = 0.003). This pattern of expression was confirmed by examination of 12 other frozen epithelial ovary carcinomas, using in situ hybridization. Immunohistochemical staining of the specimens demonstrated colocalization of gal-1, laminin-1, and fibronectin. In vitro experiments were conducted to elucidate the potential biologic role of gal-1 in ovarian cancer progression. Gal-1 protein expression and release was detected in AZ364, SK-OV-3, and AZ224, but not in OVCAR-3, AZ419, and AZ382, human ovary carcinoma cell lines. Incubation of 84BR fibroblasts with conditioned media harvested from the ovary carcinoma cell lines induced an increased expression of gal-1 in the cultured fibroblasts in all cases except AZ419 and SK-OV-3. High concentrations of gal-1 (100 μg/ml) induced significantly decreased cell proliferation in all cell lines, as defined by bromodeoxyuridine incorporation. Additionally, recombinant gal-1 induced a dose-dependent increase in in vitro adhesion of AZ224, SK-OV-3, and AZ382 cells to laminin-1; adhesion to fibronectin was increased by gal-1 in OVCAR-3, AZ224, and SK-OV-3. No effect was observed in the other cases. Our data contribute to define a role for gal-1 during the interactions between human ovary carcinoma cells and host fibroblasts.
Distinct Albino3-dependent and -independent Pathways for Thylakoid Membrane Protein Insertion*

The homologous proteins Oxa1, YidC, and Alb3 mediate the insertion of membrane proteins in mitochondria, bacteria, and chloroplast thylakoids, respectively. Depletion of YidC inEscherichia coli affects the integration of every membrane protein studied, and Alb3 has been shown previously to be required for the insertion of a signal recognition particle (SRP)-dependent protein, Lhcb1, in thylakoids. In this study we have analyzed the “global” role of Alb3 in the insertion of thylakoid membrane proteins. We show that insertion of two chlorophyll-binding proteins, Lhcb4.1 and Lhcb5, is almost totally blocked by preincubation of thylakoids with anti-Alb3 antibodies, indicating a requirement for Alb3 in the insertion pathway. Insertion of the related PsbS protein, on the other hand, is unaffected by Alb3 antibodies, and insertion of a group of SRP-independent, signal peptide-bearing proteins, PsbX, PsbW, and PsbY, is likewise completely unaffected. Proteinase K is furthermore able to completely degrade Alb3, but this treatment does not affect the insertion of these proteins. Among the thylakoid proteins studied here, Alb3 requirement correlates strictly with a requirement for stromal factors and nucleoside triphosphates. However, the majority of proteins tested do not require Alb3 or any other known form of translocation apparatus.
Identification of Measurement Differences Between English and Spanish Language Versions of the Mini-Mental State Examination: Detecting Differential Item Functioning Using MIMIC Modeling

Background:Knowledge of the extent to which measurement of adult cognitive functioning differs between Spanish and English language administrations of the Mini-Mental State Examination (MMSE) is critical for inclusive, representative, and valid research of older adults in the United States. Objectives:We sought to demonstrate the use of an item response theory (IRT) based structural equation model, that is, the MIMIC model (multiple indicators, multiple causes), to evaluate MMSE responses for evidence of differential item functioning (DIF) attributable to language of administration. Subjects:We studied participants in a dementia case registry study (n = 1546), 42% of whom were examined with the Spanish language MMSE. Results:Twelve of 21 items were identified as having significant uniform DIF. The 4 most discrepant included orientation to season, orientation to state, repeat phrase, and follow command. DIF accounted for two-thirds of the observed difference in underlying level of cognitive functioning between Spanish- and English-language administration groups. Conclusions:Failing to account for measurement differences may lead to spurious inferences regarding language group differences in level of underlying level of cognitive functioning. The MIMIC model can be used to detect and adjust for such measurement differences in substantive research.
Public Goods Provision and Sanctioning in Privileged Groups

In public-good provision, privileged groups enjoy the advantage that some of their members find it optimal to supply a positive amount of the public good. However, the inherent asymmetric nature of these groups may make the enforcement of cooperative behavior through informal sanctioning harder to accomplish. In this article, the authors experimentally investigate public-good provision in normal and privileged groups with and without decentralized punishment. The authors find that compared to normal groups, privileged groups are relatively ineffective in using costly sanctions to increase everyone's contributions. Punishment is less targeted toward strong free riders, and they exhibit a weaker increase in contributions after being punished. Thus, the authors show that privileged groups are not as privileged as they initially seem.
The Effect of Therapeutic Zinc Supplementation among Young Children with Selected Infections: A Review of the Evidence

Background Zinc deficiency is now widely recognized as a leading risk factor for morbidity and mortality and is estimated to be responsible for approximately 800,000 excess deaths annually among children under 5 years of age. Objective To evaluate the impact of zinc supplementation as an adjunct in the treatment of diarrhea, pneumonia, malaria, and tuberculosis in children under 5 years of age. Methods A comprehensive literature search of electronic databases to identify randomized, controlled trials on the topic was undertaken in January 2008. Eligible studies identified on search were reviewed by the authors and data extraction was done. Statistical analyses were performed with the use of Review Manager software. Results Current analysis of the adjunctive therapeutic benefit of zinc in acute diarrhea corroborates existing reviews and provides evidence of reduction in the duration of acute diarrhea by 0.5 day (p = .002) in children under 5 years of age. However, zinc supplementation is found to have no beneficial impact in infants under 6 months of age. A beneficial effect of zinc as an adjunctive treatment is also found in persistent diarrhea, the duration of which is reduced by 0.68 day (p < .0001). Evidence of the benefit of zinc supplementation in pneumonia and malaria is insufficient, whereas no studies are available in children with tuberculosis. Conclusions The existing literature provides evidence of a beneficial effect of therapeutic zinc supplementation in the reduction of the duration of acute and persistent diarrhea. However, evidence for its impact on pneumonia, malaria. and tuberculosis in children under 5 years of age is insufficient and needs further evaluation.
Comparison and Combination of State-of-the-art Techniques for Handwritten Character Recognition: Topping the MNIST Benchmark

Although the recognition of isolated handwritten digits has been a research topic for many years, it continues to be of interest for the research community and for commercial applications. We show that despite the maturity of the field, different approaches still deliver results that vary enough to allow improvements by using their combination. We do so by choosing four well-motivated state-of-the-art recognition systems for which results on the standard MNIST benchmark are available. When comparing the errors made, we observe that the errors made differ between all four systems, suggesting the use of classifier combination. We then determine the error rate of a hypothetical system that combines the output of the four systems. The result obtained in this manner is an error rate of 0.35% on the MNIST data, the best result published so far. We furthermore discuss the statistical significance of the combined result and of the results of the individual classifiers.
Generalized Assorted Pixel Camera: Postcapture Control of Resolution, Dynamic Range, and Spectrum

We propose the concept of a generalized assorted pixel (GAP) camera, which enables the user to capture a single image of a scene and, after the fact, control the tradeoff between spatial resolution, dynamic range and spectral detail. The GAP camera uses a complex array (or mosaic) of color filters. A major problem with using such an array is that the captured image is severely under-sampled for at least some of the filter types. This leads to reconstructed images with strong aliasing. We make four contributions in this paper: 1) we present a comprehensive optimization method to arrive at the spatial and spectral layout of the color filter array of a GAP camera. 2) We develop a novel algorithm for reconstructing the under-sampled channels of the image while minimizing aliasing artifacts. 3) We demonstrate how the user can capture a single image and then control the tradeoff of spatial resolution to generate a variety of images, including monochrome, high dynamic range (HDR) monochrome, RGB, HDR RGB, and multispectral images. 4) Finally, the performance of our GAP camera has been verified using extensive simulations that use multispectral images of real world scenes. A large database of these multispectral images has been made available at http://wwwl.cs. columbia.edu/ CAVE/projects/gap_camera/ for use by the research community.
Intravenous immunoglobulins in immunodeficiencies: more than mere replacement therapy

Intravenous immunoglobulin (IVIG) is a therapeutic compound prepared from pools of plasma obtained from several thousand healthy blood donors. For more than 20 years, IVIG has been used in the treatment of a wide range of primary and secondary immunodeficiencies. IVIG now represents a standard therapeutic option for most antibody deficiencies. Routinely, IVIG is used in patients with X‐linked agammaglobulinaemia (XLA), common variable immunodeficiency (CVID), X‐linked hyper‐IgM, severe combined immunodeficiency, Wiskott‐Aldrich syndrome, and selective IgG class deficiency. In addition, IVIG is used extensively in the treatment of a wide variety of autoimmune disorders. IVIG is administered at distinct doses in the two clinical settings: whereas immunodeficient patients are treated with replacement levels of IVIG, patients with autoimmune and inflammatory diseases are administered with very high doses of IVIG. Several lines of experimental evidence gathered in the recent years suggest that the therapeutic beneficial effect of IVIG in immunodeficiencies reflects an active role for IVIG, rather than a mere passive transfer of antibodies.
UV‐assisted three‐dimensional printing of polymer nanocomposites based on inorganic fillers

In this work, nanocomposites based on a UV-curable polymeric resin and different inorganic fillers were developed for use in UV-assisted three-dimensional (UV-3D) printing. This technology consists in the additive multilayer deposition of a UV-curable resin for the fabrication of 3D macro structures and microstructures of arbitrary shapes. A systematic investigation on the effect of filler concentration on the rheological properties of the polymer-based nanocomposites was performed. In particular, the rheological characterization of these nanocomposites allowed to identify the optimal printability parameters for these systems based on the shear rate of the materials at the extrusion nozzle. In addition, photocalorimetric measurements were used to assess the effect of the presence of the inorganic fillers on the thermodynamics and kinetics of the photocuring process of the resins. By direct deposition of homogeneous solvent-free nanocomposite dispersions of different fillers in a UV-curable polymeric resin, the effect of UV-3D printing direction, fill density, and fill pattern on the mechanical properties of UV-3D printed specimens was investigated by means of uniaxial tensile tests. Finally, examples of 3D macroarchitectures and microarchitectures, spanning features, and planar transparent structures directly formed upon UV-3D printing of such nanocomposite dispersions were reproducibly obtained and demonstrated, clearly highlighting the suitability of these nanocomposite formulations for advanced UV-3D printing applications. POLYM. COMPOS. , 38:1662–1670, 2017. © 2015 Society of Plastics Engineers
Rebrightening of XRF 030723: Further Evidence for a Two-Component Jet in a Gamma-Ray Burst

We numerically investigate optical afterglows from two-component jets under various configurations. Generally, the light curve is characterized by a rapid rebrightening when the observer is off-axis with respect to the narrow component, with the amplitude and peak time depending on detailed parameters. We further show that the optical afterglow of XRF 030723, especially its notable and rapid rebrightening, can be well explained by a typical two-component jet. This X-ray flash, together with GRB 030329, strongly hints toward the two-component jet model as a unified picture for X-ray flashes and gamma-ray bursts. With a narrow but ultrarelativistic inner outflow and a wide but less energetic outer ejecta, a two-component jet will be observed as a typical gamma-ray burst if our line of sight is within the angular scope of the narrow outflow. Otherwise, if the line of sight is within or slightly beyond the cone of the wide component, an X-ray flash will be detected.
Species Composition and Abundance of Stink Bugs (Hemiptera: Heteroptera: Pentatomidae) in Minnesota Field Corn

ABSTRACT In response to concerns of increasing significance of stink bugs (Hemiptera: Heteroptera: Pentatomidae) in northern states, a survey was conducted over 2 yr in Minnesota to characterize the Pentatomidae associated with field corn, Zea mays L. Halyomorpha halys (Stål), an exotic species, was not detected in this survey, despite continued detection of this species as an invader of human-made structures in Minnesota. Five species of Pentatomidae (four herbivorous; one predatory) were collected from corn. Across years, Euschistus variolarius (Palisot de Beauvois) and Euschistus servus euschistoides (Vollenhoven) had the greatest relative abundances and frequencies of detection. In 2012, the abundance of herbivorous species exceeded 25 nymphs and adults per 100 plants (i.e., an economic threshold) in 0.48% of fields. However, the abundance of herbivorous species did not reach economic levels in any fields sampled in 2013. The frequency of detection of herbivorous species and ratio of nymphs to adults was highest during reproductive growth stages of corn. The predator species, Podisus maculiventris (Say), was detected in 0 to 0.32% of fields. These results provide baseline information on the species composition and abundance of Pentatomidae in Minnesota field corn, which will be necessary for documentation of changes to this fauna as a result of the invasion of H. halys and to determine if some native species continue to increase in abundance in field crops.
Polycrystalline silicon thin films crystallized by green laser

A top hat beam of frequency-doubled Nd: YAG laser is obtained from our beam shaping optical system. With this beam, amorphous silicon thin films deposited on glass by plasma-enhanced chemical vapor deposition (PECVD) are successfully crystallized. The surface morphology of the laser-crystallized materials is studied by atomic force microscopy (AFM). Pronounced increase in surface roughness after the laser treatment could be observed from the Microscope Photos. Raman spectra of the Si films are measured to confirm the phase transition from amorphous to polycrystalline and to investigate the silicon structural properties. Crystalline fraction evaluated from the Raman spectra are found to increase almost linearly with the laser fluence. There exists the optimized laser fluence to produce the best crystallization in the range of 400 ~1000mJ/cm2.
Cellular titin localization in stress fibers and interaction with myosin II filaments in vitro

We previously discovered a cellular isoform of titin (originally named T-protein) colocalized with myosin II in the terminal web domain of the chicken intestinal epithelial cell brush border cytoskeleton (Eilertsen, K.J., and T.C.S. Keller. 1992. J. Cell Biol. 119:549-557). Here, we demonstrate that cellular titin also colocalizes with myosin II filaments in stress fibers and organizes a similar array of myosin II filaments in vitro. To investigate interactions between cellular titin and myosin in vitro, we purified both proteins from isolated intestinal epithelial cell brush borders by a combination of gel filtration and hydroxyapatite column chromatography. Electron microscopy of brush border myosin bipolar filaments assembled in the presence and absence of cellular titin revealed a cellular titin- dependent side-by-side and end-to-end alignment of the filaments into highly ordered arrays. Immunogold labeling confirmed cellular titin association with the filament arrays. Under similar assembly conditions, purified chicken pectoralis muscle titin formed much less regular aggregates of muscle myosin bipolar filaments. Sucrose density gradient analyses of both cellular and muscle titin-myosin supramolecular arrays demonstrated that the cellular titin and myosin isoforms coassembled with a myosin/titin ratio of approximately 25:1, whereas the muscle isoforms coassembled with a myosin:titin ratio of approximately 38:1. No coassembly aggregates were found when cellular myosin was assembled in the presence of muscle titin or when muscle myosin was assembled in the presence of cellular titin. Our results demonstrate that cellular titin can organize an isoform-specific association of myosin II bipolar filaments and support the possibility that cellular titin is a key organizing component of the brush border and other myosin II-containing cytoskeletal structures including stress fibers.
Recent progress in Glinus oppositifolius research

Abstract Context: Glinus oppositifolius (L.) Aug. DC. (Molluginaceae), a perennial subshrubs herb, grows at low altitudes in the southern part of Taiwan, and is used in traditional Chinese medicine for herpes zoster and herpangina. Objective: This study describes nutritional and therapeutic potential of Glinus oppositifolius and summarizes scientific evidence that supports traditional claims; recent progress in research for this plant is reviewed herein. Materials and methods: The literature has been retrieved from the web-based online systems including PubMed, Medline, and Google Scholar. The articles related to phytochemistry, pharmaceutical biology and ethnopharmacology have been excluded. Results and discussion: In clinical practice, the plant has been extensively investigated in a broad range of studies to provide scientific evidence for folklore claims or to find new therapeutic uses. The present review may arouse related research and make a more valid display for Taiwanese native medicinal plants.
Reactivation of hepatitis E infection in a patient with acute lymphoblastic leukaemia after allogeneic stem cell transplantation

Hepatitis E virus (HEV) is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialised countries. Generally, HEV causes an acute self-limiting hepatitis. The clinical course is characterised by transient viraemia and transaminasaemia followed by a full hepatic recovery. Recent studies describe prolonged and chronic HEV infections in some immunosuppressed patients after solid organ transplantation. Here, an indigenous acute limited hepatitis E in a patient with Philadelphia chromosome-positive acute lymphoblastic leukaemia prior to allogeneic stem cell transplantation is reported. Fourteen weeks after stem cell transplantation, reappearance of HEV viraemia was observed, with increasing viral load and modestly elevated serum transaminases. Sequence analysis of the viral RNAs revealed a reactivation of endogenous HEV genotype 3, indicating viral persistence after recovery from acute hepatitis E.