Miracle-dz/UniFAR-FTUs huggingface dataset:
Risk Classification for Enteroviral Infection in Children With Meningitis and Negative Gram Stain

Objectives Enterovirus is the most common cause of aseptic meningitis in children. This study aimed at identifying baseline variables associated with a positive cerebrospinal fluid (CSF) Enterovirus polymerase chain reaction (PCR) to aid clinicians in targeting patients who could be tested and treated as outpatients. Methods We performed a retrospective review of children (2 months to 17 years old) admitted to the Children's Memorial Hermann Hospital in Houston, TX, between January 2005 and December 2010 with symptoms of meningitis, CSF white cell count of greater than 5 cells/mm3, and a negative CSF Gram stain, who had a CSF Enterovirus PCR. Results One hundred thirty-seven children were reviewed; median age was 4.7 (0.1–17.1) years, and 79 (58%) were male. Fifty patients (37%) had positive CSF Enterovirus PCR. Only 13 (15%) of the Enterovirus PCR-negative patients had an identifiable etiology. All patients were hospitalized. The mean hospital stay for patients with Enterovirus was 2.9 days; 88% received empiric antibiotics. Rates of antibiotic administration were not different between PCR-positive and PCR-negative groups (P > 0.05). All patients with Enterovirus had a favorable clinical outcome. A predictive model was created using 3 baseline variables independently associated with a positive Enterovirus PCR (P < 0.05): May to November presentation, CSF protein of less than 100 mg/dL, and absence of focal neurologic signs. The model classified patients into 2 risk categories for a positive Enterovirus PCR (low risk, 0% [0/17 patients]; high risk, 42% [50/120 patients]; P < 0.001). Conclusions Our predictive model can be used to identify children for whom Enterovirus PCR testing is warranted. Such testing could avoid unnecessary hospitalization and antibiotic administration.
Multilingual domain modeling in Twenty-One: automatic creation of a bi-directional translation lexicon from a parallel corpus

Within the project Twenty-One, which aims at the effective dissemination of information on ecology and sustainable development, a sytem is developed that supports cross-language information retrieval in any of the four languages Dutch, English, French and German. Knowledge of this application domain is needed to enhance existing translation resources for the purpose of lexical disambiguation. This paper describes an algorithm for the automated acquisition of a translation lexicon from a parallel corpus. New about the presented algorithm is the statistical language model used. Because the algorithm is based on a symmetric translation model it becomes possible to identify one-to-many and many-to-one relations between words of a language pair. We claim that the presented method has two advantages over algorithms that have been published before. Firstly, because the translation model is more powerful, the resulting bilingual lexicon will be more accurate. Secondly, the resulting bilingual lexicon can be used to translate in both directions between a language pair. Different versions of the algorithm were evaluated on the Dutch and English version of the Agenda 21 corpus, which is a UN document on the application domain of sustainable development.
Formant Bandwidth Affects the Identification of Competing Vowels

Formant bandwidth is known to have little effect on vowel quality. This paper shows that it has a strong effect on mutual masking between vowels. Subjects presented with stimuli consisting of pairs of synthetic vowels were requested to report one or two vowels for each stimulus. Identification rates were calculated independently for both vowels in the stimulus. Vowels had either the same or different fundamental frequencies. Their RMS amplitudes differed by 5, 15 or 25 dB. Formant bandwidth of each vowel was either twice or half its standard value. Identification of a target vowel was best when: (1) its RMS amplitude exceeded that of its competitor, (2) its formants were narrow, (3) formants of the competitor were wide, and (4) F0s were different. These effects were approximately orthogonal. A narrow-bandwidth voice is thus more resistant to masking, and a stronger masker, than a wide-formant vowel.
Spawning origins of pelagic juvenile cod Gadus morhua inferred from spatially explicit age distributions : potential influences on year-class strength and recruitment

Incongruity between the reconstructed spawning period of pelagic juvenile (0-group) cod Gadus morhua and the observed spawning period for adult cod on the main spawning grounds south of Iceland have indicated that large numbers of the surviving juvenile population may, in some years, originate from other smaller inshore spawning grounds located within fjords of the west, north and east coasts. We examine this hypothesis by using age/length and temperature/growth relation- ships, based on aging of 0-group cod from 1995 and 1997, to analyze historical demographic data from 0-group fish surveys (1970 to 1998) and determine the spawning distribution and origin of pelagic juvenile cod. Data on early life-history characteristics in conjunction with temporal and spa- tial distribution of pelagic juvenile cod are used to create new abundance indices representing the inshore and offshore juvenile cod-population components. Partitioning of the historical 0-group index into these population components at a finer geographic scale than has been used previously provides new information for estimating the relative contribution of the main spawning component versus the smaller in-fjord spawning components towards variable recruitment levels in the Icelandic cod stock. Spawning diversity, or multiple spawning components, are important for recruitment success as they disperse the mortality risk of the early life-history stages, which are thought to be the principal deter- minants of year-class strength.
Rumination in Adolescence: the Distinctive Impact of Brooding and Reflection on Psychopathology

Abstract Rumination has a crucial role in the onset, severity and maintenance of depression in adolescent and adult populations. The Ruminative Responses Scale (RRS) is the most widely self-report instrument used to assess individual differences in the tendency to engage in ruminative responses style. This paper aims to test the factor structure of the 10-item RRS and the gender-based measurement invariance, in a community sample of adolescents, using a Confirmatory Factor Analysis. Participants were 542 adolescents (53% females) with a mean age of 14 years old (SD = 1.75) from middle and secondary schools (years of education’s mean = 9.46, SD = 1.60) in Portugal. Results confirm the two-factor structure of the RRS composed by brooding and reflection dimensions (GFI = .93, CFI = .90, TLI = .87, SRMR = .05, RMSEA = .11, 90% C.I. [0.092 to 0.121]) and the invariance across gender (GFI = .91, CFI = .89, TLI = .85, RMSEA = .08, 90% C.I. [0.069 to 0.090], p < .001). RRS and their dimensions presented a good internal reliability (Brooding: α = .80; Reflection: α = .75; RRS total: α = .85). Brooding and reflection dimensions revealed moderate correlations with depression, anxiety and stress symptoms (p < .001). Multiple Regression Analysis through Structural Equation Modelling (SEM) showed that brooding is significantly and strongly associated with internalizing symptoms (p < .001). Female adolescents reported more levels of rumination than male adolescents. Overall, these findings support the usefulness of the Portuguese version of RRS and suggest that this short version is an economical, valid and reliable measure to assess ruminative response styles in adolescence.
SpaceTop: integrating 2D and spatial 3D interactions in a see-through desktop environment

SpaceTop is a concept that fuses spatial 2D and 3D interactions in a single workspace. It extends the traditional desktop interface with interaction technology and visualization techniques that enable seamless transitions between 2D and 3D manipulations. SpaceTop allows users to type, click, draw in 2D, and directly manipulate interface elements that float in the 3D space above the keyboard. It makes it possible to easily switch from one modality to another, or to simultaneously use two modalities with different hands. We introduce hardware and software configurations for co-locating these various interaction modalities in a unified workspace using depth cameras and a transparent display. We describe new interaction and visualization techniques that allow users to interact with 2D elements floating in 3D space. We present the results from a preliminary user study that indicates the benefit of such hybrid workspaces.
Dark Matter Annihilation Signals: The Importance of Radiative Corrections

Being able to safely distinguish astrophysical from potential dark matter (DM) annihilation signals is of utmost importance for indirect DM searches. To this end, one has to rely on distinctive -- and unique -- spectral signatures to look for. Internal bremsstrahlung (IB), unavoidable in the presence of charged annihilation products, provides such a signature. In fact, as it generically dominates the gamma-ray spectrum expected from DM annihilations, at high energies, it may well turn out to be more important for indirect DM searches than the traditionally looked-for line signals. As illustrated in some detail, the observation of IB signatures would even allow to distinguish between different DM candidates or to constrain significantly the parameter space of, e.g., neutralino DM. The gamma-ray contributions reported here are therefore of great interest for the already launched Fermi/GLAST satellite and the upcoming new generation of Air Cherenkov Telescope systems like CTA -- which are most sensitive at the high energies where these effects are particularly important. Finally, radiative corrections may even significantly alter the positron spectrum from DM annihilations; an intriguing positron excess recently found by the PAMELA satellite might turn out to be an indication of the peculiar spectral signature expected in that case.
Calentamiento por radiofrecuencia para esterilizar Pinus radiata como material para embalajes. Parte 1: Tiempo total de tratamiento

In this work the total heating time was studied using radio frequency to sterilize wood packaging material of Pinus radiata, through the conditions 56 ° C and 60 ° C for 30 and 1 minutes respectively, according to international phytosanitary norms. The experimental tests were carried out in a laboratory radiofrequency equipment of 3 m3 capacity. The heating time was determined and prediction models were developed, depending on the thickness, plaque separation and wood loading volume, with confidence levels above 90%. The results showed that the total time of radiofrequency sterilization was greater in the surface of the pile and were statistically similar under both treatment conditions.
Compatibilization and mechanical properties of compression-molded polypropylene/high-impact polystyrene blends

This research work focused on polypropylene/high-impact polystyrene polymer blends. Since this blend (80/20 in weight) was incompatible, styrene–ethylene–butylene–styrene (SEBS) block copolymer, styrene–butadiene–styrene (SBS), and ethylene vinyl acetate were tested as compatibilizers (2.5 or 7.5 wt% in relation to blend). After blending via extrusion, the compositions were compression molded and their mechanical (tensile and impact), morphological (scanning electron microscopy), and rheological (melt flow index and viscosity) properties were evaluated. Blends containing SBS and 7.5 wt% of SEBS showed a positive effect regarding impact strength, although with a decrease in Young’s modulus and tensile strength. Rheological characteristics were also affected by the compatibilizers.
Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P‐selectin and E‐selectin

To study rolling of mouse neutrophils on P‐ and E‐selectins in whole blood and without cell isolation, we constructed an autoperfused flow chamber made from rectangular microslides (0.2×2 mm) perfused from a carotid artery catheter. A differential pressure transducer served to measure wall shear stress. Green fluorescent neutrophils rolled on P‐selectin but not E‐selectin coated at 50 ng/ml, with some rolling on E‐selectin at 150 ng/ml. However, when P‐ and E‐selectins were coimmobilized, the resulting number of rolling neutrophils was sixfold and fourfold higher than on P‐ or E‐selectin alone. Velocity and flux analysis shows that P‐selectin initiates neutrophil rolling, and a small amount of E‐selectin, unable to capture many neutrophils, reduces the rolling velocity of all neutrophils by more than 90%. The unexpected synergism between E‐ and P‐selectins explains why neutrophil recruitment is enhanced when both selectins are expressed.
Mutations in the membrane-spanning domain of the human immunodeficiency virus envelope glycoprotein that affect fusion activity

A chimeric protein consisting of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) ectodomain joined to the transmembrane and cytoplasmic-tail domains of vesicular stomatitis virus G protein lost the ability to fuse CD4+ HeLa cells yet was transported to the cell surface and cleaved normally. These results suggested some critical role of the HIV gp41 transmembrane or cytoplasmic domain in fusion. Subsequent mutagenic analysis of the HIV-1 Env transmembrane domain revealed that the sequence of amino acid residues from positions 696 to 707 of the transmembrane domain was important for fusion function but was not required for anchoring of the Env protein in the lipid bilayer or for transport to the cell surface. Further analysis indicated that the basic residues at positions 696 and 707 were critical for membrane fusion activity, as was the spacing between these residues. These results demonstrate that in addition to providing an anchoring function, the specific amino acid sequence in the transmembrane domain plays a crucial role in the membrane fusion process.
Dual Role of Interleukin-4 (IL-4) in Pulmonary Paracoccidioidomycosis: Endogenous IL-4 Can Induce Protection or Exacerbation of Disease Depending on the Host Genetic Pattern

ABSTRACT Resistance to paracoccidioidomycosis, the most important endemic mycosis in Latin America, is thought to be primarily mediated by cellular immunity and the production of gamma interferon. To assess the role of interleukin-4 (IL-4), a Th2 cytokine, pulmonary paracoccidioidomycosis in IL-4-depleted susceptible (B10.A) and intermediate (C57BL/6) mice was studied. Two different protocols were used to neutralize endogenous IL-4 in B10.A mice: 1 mg of anti-IL-4 monoclonal antibody (MAb)/week and 8 mg 1 day before intratracheal infection with 106Paracoccidioides brasiliensis yeast cells. Unexpectedly, both protocols enhanced pulmonary infection but did not alter the levels of pulmonary cytokines and specific antibodies. Since in a previous work it was verified that C57BL/6 mice genetically deficient in IL-4 were more resistant to P. brasiliensis infection, we also investigated the effect of IL-4 depletion in this mouse strain. Treatment with the MAb at 1 mg/week led to less severe pulmonary disease associated with impaired synthesis of Th2 cytokines in the lungs and liver of control C57BL/6 mice. Conversely, in IL-4-depleted C57BL/6 mice, increased levels of tumor necrosis factor alpha and IL-12 were found in the lungs and liver, respectively. In addition, higher levels of immunoglobulin G2a (IgG2a) and lower levels of IgG1 antibodies were produced by IL-4-depleted mice than by control mice. Lung pathologic findings were equivalent in IL-4-depleted and untreated B10.A mice. In IL-4-depleted C57BL/6 mice, however, smaller and well-organized granulomas replaced the more extensive lesions that developed in untreated mice. These results clearly showed that IL-4 can have a protective or a disease-promoting effect in pulmonary paracoccidioidomycosis depending on the genetic background of the host.
A Novel Mutation within the Central Listeria monocytogenes Regulator PrfA That Results in Constitutive Expression of Virulence Gene Products

ABSTRACT The PrfA protein of Listeria monocytogenes functions as a key regulatory factor for the coordinated expression of many virulence genes during bacterial infection of host cells. PrfA activity is controlled by multiple regulatory mechanisms, including an apparent requirement for either the presence of a cofactor or some form of posttranslational modification that regulates the activation of PrfA. In this study, we describe the identification and characterization of a novel PrfA mutation that results in constitutive activation of the PrfA protein. The PrfA L140F mutation was found to confer high-level expression of PrfA-regulated genes and to be functionally dominant over the wild-type allele. The presence of the PrfA L140F mutation resulted in the aggregation of L. monocytogenes in broth culture and, unlike previously described prfA mutations, appeared to be slightly toxic to the bacteria. High-level PrfA-dependent gene expression showed no additional increase in L. monocytogenes strains containing an additional copy of prfA L140F despite a >4-fold increase in PrfA protein levels. In contrast, the introduction of multiple copies of the wild-type prfA allele to L. monocytogenes resulted in a corresponding increase in PrfA-dependent gene expression, although overall expression levels remained far below those observed for PrfA L140F strains. These results suggest a hierarchy of PrfA regulation, such that the relative levels of PrfA protein present within the cell correlate with the levels of PrfA-dependent gene expression when the protein is not in its fully activated state; however, saturating levels of the protein are then quickly reached when PrfA is converted to its active form. Regulation of the PrfA activation status must be an important facet of L. monocytogenes survival, as mutations that result in constitutive PrfA activation may have deleterious consequences for bacterial physiology.
Epidemiological, clinical and laboratory features of patients hospitalized with 2009 pandemic influenza in north of Iran

BACKGROUND The clinical manifestations and outcome of influenza infection differ between various patients in the world. The purpose of this study was to assess the clinical manifestations of patients with confirmed or suspected novel H1N1 flu infection in Sari, North of Iran. METHODS From September 2009 to January 2010, the patients' data were collected by retrospective chart review of medical records. Laboratory confirmation included a positive RT-PCR (reverse transcriptase-polymerase-chain-reaction assay) from a nasal or pharyngeal swab sample. RESULTS Nearly 80% of established patients were in age group of 15-45 years. Approximately 14.6% of female cases were pregnant There was no significant difference in clinical and laboratory characteristics of patients with confirmed H1N1 virus infection to total cases with Influenza Like Illness (ILI). Thirty nine (95.1%) of the established patients had a combination of fever plus sore throat or cough. Relative lymphopenia was reported in 36.6%. Pneumonia was the most common complication. Acute pericarditis evolved in one case and aseptic meningitis was reported in another. CONCLUSION Precise collecting information of clinical manifestations, risk factors and other characteristics of flu, can help to the early infection detection, timely treatment of patients and proper preventive measurements.
Is GPR17 a P2Y/Leukotriene Receptor? Examination of Uracil Nucleotides, Nucleotide Sugars, and Cysteinyl Leukotrienes as Agonists of GPR17

The orphan receptor GPR17 has been reported to be activated by UDP, UDP-sugars, and cysteinyl leukotrienes, and coupled to intracellular Ca2+ mobilization and inhibition of cAMP accumulation, but other studies have reported either a different agonist profile or lack of agonist activity altogether. To determine if GPR17 is activated by uracil nucleotides and leukotrienes, the hemagglutinin-tagged receptor was expressed in five different cell lines and the signaling properties of the receptor were investigated. In C6, 1321N1, or Chinese hamster ovary (CHO) cells stably expressing GPR17, UDP, UDP-glucose, UDP-galactose, and cysteinyl leukotriene C4 (LTC4) all failed to promote inhibition of forskolin-stimulated cAMP accumulation, whereas both UDP and UDP-glucose promoted marked inhibition (>80%) of forskolin-stimulated cAMP accumulation in C6 and CHO cells expressing the P2Y14 receptor. Likewise, none of these compounds promoted accumulation of inositol phosphates in COS-7 or human embryonic kidney 293 cells transiently transfected with GPR17 alone or cotransfected with Gαq/i5, which links Gi-coupled receptors to the Gq-regulated phospholipase C (PLC) signaling pathway, or PLCε, which is activated by the Gα12/13 signaling pathway. Moreover, none of these compounds promoted internalization of GPR17 in 1321N1-GPR17 cells. Consistent with previous reports, coexpression experiments of GPR17 with cysteinyl leukotriene receptor 1 (CysLTR1) suggested that GPR17 acts as a negative regulator of CysLTR1. Taken together, these data suggest that UDP, UDP-glucose, UDP-galactose, and LTC4 are not the cognate ligands of GPR17.
Spreading characteristics of an insoluble surfactant film on a thin liquid layer: comparison between theory and experiment

We describe measurements of the surface slope and reconstruction of the interface shape during the spreading of an oleic acid film on the surface of a thin aqueous glycerol mixture. This experimental system closely mimics the behaviour of an insoluble surfactant film driven to spread on a thin viscous layer under the action of a tangential (Marangoni) surface stress. Refracted image Moiré topography is used to monitor the evolution of the surface slope over macroscopic distances, from which the time variant interface shape and advancing speed of the surfactant film are inferred. The interfacial profile exhibits a strong surface depression ahead of the surfactant source capped by an elevated rim at the surfactant leading edge. The surface slope and shape as well as the propagation characteristics of the advancing rim can be compared directly with theoretical predictions. The agreement is quite strong when the model allows for a small level of pre-existing surface contamination of the initial liquid layer. Comparison between theoretical and experimental profiles reveals the importance of the initial shear stress in determining the evolution in the film thickness and surfactant distribution. This initial stress appears to thin the underlying liquid support so drastically that the surfactant droplet behaves as a finite and not an infinite source, even though there is always an excess of surfactant present at the origin.
Cardiovascular Disease in Ageing: An Overview on Thoracic Aortic Aneurysm as an Emerging Inflammatory Disease

Medial degeneration associated with thoracic aortic aneurysm and acute aortic dissection was originally described by Erdheim as a noninflammatory lesion related to the loss of smooth muscle cells and elastic fibre fragmentation in the media. Recent evidences propose the strong role of a chronic immune/inflammatory process in aneurysm evocation and progression. The coexistence of inflammatory cells with markers of apoptotic vascular cell death in the media of ascending aorta with aneurysms and type A dissections raises the possibility that activated T cells and macrophages may contribute to the elimination of smooth muscle cells and degradation of the matrix. On the other hand, several inflammatory pathways (including TGF-β, TLR-4 interferon-γ, chemokines, and interferon-γ) seem to be involved in the medial degeneration related to aged and dilated aorta. This is an overview on thoracic aortic aneurysm as an emerging inflammatory disease.