Miracle-dz/UniFAR-FTUs huggingface dataset:
DNA methylation profiling reveals a pathological signature that contributes to transcriptional defects of CD34+ CD15− cells in early chronic‐phase chronic myeloid leukemia

Despite the high efficiency of tyrosine kinase inhibitors (TKI), some patients with chronic myeloid leukemia (CML) will display residual disease that can become resistant to treatment, indicating intraclonal heterogeneity in chronic‐phase CML (CP‐CML). To determine the basis of this heterogeneity, we conducted the first exhaustive characterization of the DNA methylation pattern of sorted CP‐CML CD34+CD15− (immature) and CD34−CD15+ (mature) cells at diagnosis (prior to any treatment) and compared it to that of CD34+CD15− and CD34−CD15+ cells isolated from healthy donors (HD). In both cell types, we identified several hundreds of differentially methylated regions (DMRs) showing DNA methylation changes between CP‐CML and HD samples, with only a subset of them in common between CD34+CD15− and CD34−CD15+ cells. This suggested DNA methylation variability within the same CML clone. We also identified 70 genes that could be aberrantly repressed upon hypermethylation and 171 genes that could be aberrantly expressed upon hypomethylation of some of these DMRs in CP‐CML cells, among which 18 and 81, respectively, were in CP‐CML CD34+CD15− cells only. We then validated the DNA methylation and expression defects of selected candidate genes. Specifically, we identified GAS2, a candidate oncogene, as a new example of gene the hypomethylation of which is associated with robust overexpression in CP‐CML cells. Altogether, we demonstrated that DNA methylation abnormalities exist at early stages of CML and can affect the transcriptional landscape of malignant cells. These observations could lead to the development of combination treatments with epigenetic drugs and TKI for CP‐CML.
Anti‐HLA alloantibodies of the IgA isotype in re‐transplant candidates part II: Correlation with graft survival

We reported previously on the widespread occurrence of anti‐HLA alloantibodies of the IgA isotype (anti‐HLA IgA) in the sera of solid‐organ re‐transplantation (re‐tx) candidates (Arnold et al., ). Specifically focussing on kidney re‐tx patients, we now extended our earlier findings by examining the impact of the presence and donor specificity of anti‐HLA IgA on graft survival. We observed frequent concurrence of anti‐HLA IgA and anti‐HLA IgG in 27% of our multicenter collective of 694 kidney re‐tx patients. This subgroup displayed significantly reduced graft survival as evidenced by the median time to first dialysis after transplantation (TTD 77 months) compared to patients carrying either anti‐HLA IgG or IgA (TTD 102 and 94 months, respectively). In addition, donor specificity of anti‐HLA IgA had a significant negative impact on graft survival (TTD 74 months) in our study. Taken together, our data strongly indicate that presence of anti‐HLA IgA, in particular in conjunction with anti‐HLA‐IgG, in sera of kidney re‐tx patients is associated with negative transplantation outcome.
Protective Effects of Topiroxostat on an Ischemia-Reperfusion Model of Rat Hearts.

BACKGROUND Ischemia/reperfusion (I/R) injury triggers cardiac dysfunctions via creating reactive oxygen species (ROS). Because xanthine oxidase (XO) is one of the major enzymes that generate ROS, inhibition of XO is expected to suppress ROS-induced I/R injury. However, it remains unclear whether XO inhibition really yields cardioprotection during I/R. The protective effects of the XO inhibitors, topiroxostat and allopurinol, on cardiac I/R injury were evaluated.Methods and Results:Using isolated rat hearts, ventricular functions, occurrence of arrhythmias, XO activities and thiobarbituric acid reactive substances (TBARS) productions and myocardial levels of adenine nucleotides before and after I/R, and cardiomyocyte death markers during reperfusion, were evaluated. Topiroxostat prevented left ventricular dysfunctions and facilitated recovery from arrhythmias during I/R. Allopurinol and the antioxidant, N-acetylcysteine (NAC), exhibited similar effects at higher concentrations. Topiroxostat inhibited myocardial XO activities and TBARS productions after I/R. I/R decreased myocardial levels of ATP, ADP and AMP, but increased that of xanthine. While topiroxostat, allopurinol or NAC did not change myocardial levels of ATP, ADP or AMP after I/R, all of the agents decreased the level of xanthine. They also decreased releases of CPK and LDH during reperfusion. CONCLUSIONS Topiroxostat showed protective effects against I/R injury with higher potency than allopurinol or NAC. It dramatically inhibited XO activity and TBARS production, suggesting suppression of ROS generation.
Pentocin MQ1: A Novel, Broad-Spectrum, Pore-Forming Bacteriocin From Lactobacillus pentosus CS2 With Quorum Sensing Regulatory Mechanism and Biopreservative Potential

Micrococcus luteus, Listeria monocytogenes, and Bacillus cereus are major food-borne pathogenic and spoilage bacteria. Emergence of antibiotic resistance and consumer demand for foods containing less of chemical preservatives led to a search for natural antimicrobials. A study aimed at characterizing, investigating the mechanism of action and regulation of biosynthesis and evaluating the biopreservative potential of pentocin from Lactobacillus pentosus CS2 was conducted. Pentocin MQ1 is a novel bacteriocin isolated from L. pentosus CS2 of coconut shake origin. The purification strategy involved adsorption-desorption of bacteriocin followed by RP-HPLC. It has a molecular weight of 2110.672 Da as determined by MALDI-TOF mass spectrometry and a molar extinction value of 298.82 M−1 cm−1. Pentocin MQ1 is not plasmid-borne and its biosynthesis is regulated by a quorum sensing mechanism. It has a broad spectrum of antibacterial activity, exhibited high chemical, thermal and pH stability but proved sensitive to proteolytic enzymes. It is potent against M. luteus, B. cereus, and L. monocytogenes at micromolar concentrations. It is quick-acting and exhibited a bactericidal mode of action against its targets. Target killing was mediated by pore formation. We report for the first time membrane permeabilization as a mechanism of action of the pentocin from the study against Gram-positive bacteria. Pentocin MQ1 is a cell wall-associated bacteriocin. Application of pentocin MQ1 improved the microbiological quality and extended the shelf life of fresh banana. This is the first report on the biopreservation of banana using bacteriocin. These findings place pentocin MQ1 as a potential biopreservative for further evaluation in food and medical applications.
FinDroidHR

We present FinDroidHR, a novel gesture input technique for off-the-shelf smartwatches. Our technique is designed to detect 10 hand gestures on the hand wearing a smartwatch. The technique is enabled by analysing features of the Photoplethysmography (PPG) signal that optical heart-rate sensors capture. In a study with 20 participants, we show that FinDroidHR achieves 90.55% accuracy and 90.73% recall. Our work is the first study to explore the feasibility of using optical sensors on the off-the-shelf wearable devices to recognise gestures. Without requiring bespoke hardware, FinDroidHR can be readily used on existing smartwatches.
Comparing depressive symptoms in teenage boys and girls

Introduction: Symptoms of depression vary between the males and females. Depressed men show behaviors such as irritability, restlessness, difficulty in concentrating, and instead of the usual behaviors. Sleep disturbance is a common symptom in depressed men. Men are less likely to go to doctors and unconsciously show other behaviors such as anger instead of the sadness. It seems that considering depression as “feminine” is a great injustice toward male patients whom their illness will not be diagnosed nor treated. Materials and Methods: The sample consisted of 191 depressed adolescents, 108 males and 83 females aged 13–19 years old. Data collected for 10 years from 2005 to 2015 and their depressive symptoms were evaluated by the Beck Depression Inventory-Second Edition. Results: Depressed girls felt sadness, guilt, punishment, worthlessness, low energy and fatigue, or more asthenia, whereas depressed boys have symptoms such as irritability, depression, suicidal thoughts, or desires to reduce their pleasure. The results of t-test showed that the difference between the total scores of boys and girls with depressive disorder (16.93) is significant at 0.001. F values for feeling sad (58.13), hatred of self (12.38), suicidal thoughts or desires (12.97), restlessness (17.35), and irritability (46. 41) were significant in the 0.001. Conclusion: Experiencing depression in boys and girls according to the role of gender was different. Gender can have an effective role in showing depression symptoms in adolescents.
Predicting age and gender in online social networks

A common characteristic of communication on online social networks is that it happens via short messages, often using non-standard language variations. These characteristics make this type of text a challenging text genre for natural language processing. Moreover, in these digital communities it is easy to provide a false name, age, gender and location in order to hide one's true identity, providing criminals such as pedophiles with new possibilities to groom their victims. It would therefore be useful if user profiles can be checked on the basis of text analysis, and false profiles flagged for monitoring. This paper presents an exploratory study in which we apply a text categorization approach for the prediction of age and gender on a corpus of chat texts, which we collected from the Belgian social networking site Netlog. We examine which types of features are most informative for a reliable prediction of age and gender on this difficult text type and perform experiments with different data set sizes in order to acquire more insight into the minimum data size requirements for this task.
Effects of continued psychological care toward brain tumor patients and their family members' negative emotions

Purpose: Numerous studies have confirmed that brain tumor patients and their family members frequently exhibit negative emotional reactions, such as anxiety and depression, during diagnosis and treatment of the disease. Family members experience increasing pressure as the year of survival of patient progress. The aim of this study was to investigate the effects of the continued psychological care (CPC) toward the brain tumor patients and their family members' emotions. Subjects and Methods: The asynchronous clinical control trial was performed, and 162 brain tumor patients and their family members were divided into the control group and the intervention group. The control group was only performed the telephone follow-up toward the patients. Beside this way, the intervention group was performed the CPC toward the patients and their family member. The self-rating anxiety scale (SAS) and the self-rating depression scale (SDS) were used to measure the negative emotions of the patients and their family members, and the patients' treatment compliance and the incidence of seizures were compared. Results: The SAS and SDS scores of the intervention group on the 14 days, 28 days and 3 months of the CPC were significantly lower than the control group (P < 0.05); the SAS and SDS scores of the intervention group after the intervention were significantly lower than those at the discharging (P < 0.05), the treatment compliance of the intervention group was significantly higher than the control group (P < 0.05), and the seizure incidence of the intervention group was significantly lower than the control group (P < 0.05). Conclusion: The CPC could effectively reduce the anxiety and depression among the brain tumor patients and their family members.
Cluster formation versus star formation rates around six regions in the Large Magellanic Cloud

The stellar population and star clusters around six regions in the Large Magellanic Cloud (LMC) are studied to understand the correlation between star formation and cluster formation rates. We used the stellar data base of the OGLE II LMC survey and the star cluster catalogues. The observed distributions of stellar density in the colour−magnitude diagrams (CMDs) were compared with synthetic ones generated from stellar evolutionary models. By minimising the reduced χ 2 values, the star formation history of the regions were obtained in terms of star formation rates (SFR). All the regions were found to show large SFRs between the ages 500−2 Gyr with lower values for younger and older ages. A correlated peak in the cluster and SFRs is found for ages ∼1 Gyr, and for ages less than 100 Myr. Five of the six regions show significant cluster formation in the age range of 100−300 Myr, when the SFRs were found to be very low. This indicates anti-correlation between star and cluster formation rates for the 100−300 Myr age range. A possible reason may be that the stars are predominantly formed in clusters, whether bound or unbound, as a result of star formation during the above age range. The enhanced cluster formation rate in the 100−300 Myr age range could be correlated with the encounter of the LMC with the Small Magellanic Cloud, while the enhanced star and cluster formation at ∼1 Gyr does not correspond to any interaction. This could indicate that the star formation induced by interactions is biased towards group or cluster formation of stars.
Experimental and numerical investigation of flexural behavior of carbon fiber reinforced aluminum laminates

Fiber metal laminates (FML) combine the strength of carbon fiber composite layer with ductility of the aluminum layer for desirable mechanical characteristics. For these composites, progressive failure behavior can be complex and require attention. In this study, two carbon fiber reinforced aluminum laminates (CARRAL) with a 3/2 configuration, with aluminum in the outer layer for the first case and one with carbon fiber composite layer in the outer layer were prepared using a vacuum press without any adhesive layer between the layers—a significant departure from similar aerospace materials. Epoxy from the prepreg provides adequate adhesion during consolidation in these lower cost FMLs with a pressure level of 0.35 MPa. Three-point flexural behaviors of these two material systems were evaluated under static loading and failure modes were recorded. Primary failure modes observed were crack in lower aluminum layer, carbon fiber (CFRP) layer fracture and delamination between upper aluminum and CFRP layer. A major contribution of this study was to predict the flexural response of these FMLs using LS-DYNA finite element code. Modeling the progressive damage behavior of FML by considering stress based material failure and shear stress based delamination failure between adjacent layers were key aspects of finite element modeling. Predicted mechanical behavior matches well with experimental results and the progressive nature of damage are recovered in the model.
Faraday instability of a two-layer liquid film with a free upper surface

The classic Faraday instability in a one-layer liquid film is extended to a two-layer film consisting of a lower liquid layer, supported by a vibrating solid plate, overlaid by a second layer of immiscible fluid with a free upper surface. A detailed analytical and numerical analysis of the linear stability of the film, and the interplay between the Faraday and the Rayleigh-Taylor instabilities, is presented.
Curiosity killed the cat: no evidence of an association between cat ownership and psychotic symptoms at ages 13 and 18 years in a UK general population cohort

Background Congenital or early life infection with Toxoplasma gondii has been implicated in schizophrenia aetiology. Childhood cat ownership has been hypothesized as an intermediary marker of T. gondii infection and, by proxy, as a risk factor for later psychosis. Evidence supporting this hypothesis is, however, limited. Method We used birth cohort data from the Avon Longitudinal Study of Parents and Children (ALSPAC) to investigate whether cat ownership in pregnancy and childhood (ages 4 and 10 years) was associated with psychotic experiences (PEs) in early (age 13, N = 6705) and late (age 18, N = 4676) adolescence, rated from semi-structured interviews. We used logistic regression to examine associations between cat ownership and PEs, adjusting for several sociodemographic and socioeconomic factors, household characteristics and dog ownership. Missing data were handled via multiple imputation. Results Cat ownership during pregnancy was not associated with PEs at age 13 years [adjusted odds ratio (OR) 1.15, 95% confidence interval (CI) 0.97–1.35] or 18 years (OR 1.08, 95% CI 0.86–1.35). Initial univariable evidence that cat ownership at ages 4 and 10 years was associated with PEs at age 13 years did not persist after multivariable adjustment (4 years: OR 1.18, 95% CI 0.94–1.48; 10 years: OR 1.12, 95% CI 0.92–1.36). There was no evidence that childhood cat ownership was associated with PEs at age 18 years. Conclusions While pregnant women should continue to avoid handling soiled cat litter, given possible T. gondii exposure, our study strongly indicates that cat ownership in pregnancy or early childhood does not confer an increased risk of later adolescent PEs.
What Makes Smartphone Use Meaningful or Meaningless?

Prior research indicates that many people wish to limit aspects of their smartphone use. Why is it that certain smartphone use feels so meaningless? We examined this question by using interviews, the experience sampling method, and mobile logging of 86,402 sessions of app use. One motivation for use (habitual use to pass the time) and two types of use (entertainment and passive social media) were associated with a lower sense of meaningfulness. In interviews, participants reported feeling a loss of autonomy when using their phone in these ways. These reports were corroborated by experience sampling data showing that motivation to achieve a specific purpose declined over the course of app use, particularly for passive social media and entertainment usage. In interviews, participants pointed out that even when smartphone use itself was meaningless, it could sometimes still be meaningful in the context of broader life as a 'micro escape' from negative situations. We discuss implications for how mobile apps can be used and designed to reduce meaningless experiences.
Noninvasive reconstruction of placental methylome from maternal plasma DNA: Potential for prenatal testing and monitoring

During human pregnancy, the DNA methylation of placental tissue is highly relevant to the normal growth and development of the fetus; therefore, methylomic analysis of the placental tissue possesses high research and clinical value in prenatal testing and monitoring. Thus, our aim is to develop an approach for reconstruction of the placental methylome, which should be completely noninvasive and achieve high accuracy and resolution.
Molecular mechanisms and physiological relevance of RGK proteins in the heart

The primary route of Ca2+ entry into cardiac myocytes is via 1,4‐dihydropyridine‐sensitive, voltage‐gated L‐type Ca2+ channels. Ca2+ influx through these channels influences duration of action potential and engages excitation‐contraction (EC) coupling in both the atria and the myocardium. Members of the RGK (Rad, Rem, Rem2 and Gem/Kir) family of small GTP‐binding proteins are potent, endogenously expressed inhibitors of cardiac L‐type channels. Although much work has focused on the molecular mechanisms by which RGK proteins inhibit the CaV1.2 and CaV1.3 L‐type channel isoforms that expressed in the heart, their impact on greater cardiac function is only beginning to come into focus. In this review, we summarize recent findings regarding the influence of RGK proteins on normal cardiac physiology and the pathological consequences of aberrant RGK activity.
Field Estimation of Ventilation Capacity Using FANS

Instrumentation and procedures have been developed to characterize mechanical ventilation system capacity as part of an evaluation of ammonia emissions from commercial poultry housing. A FANS anemometer array unit, developed at the Mississippi USDA center, built and refined at University of Kentucky, and calibrated at the BESS laboratory, was found to have repeatability in the range of about 1% between two traverse readings performed one after the other. The unit was used to measure broiler house fans under typical system static pressure differences. A hydraulic lift cart was fabricated to streamline FANS positioning and movement through the large poultry houses. Taping all gaps between the FANS unit and fan housing improved airflow measurements about 6% versus not taping. Using a duct to transition down to 36-inch fans resulted in a 2.5% improvement versus not using a duct. Fan manufacturer performance data was 2 to 13% higher than actual field performance.
Needs and preferences among patients with high‐grade glioma and their caregivers – A longitudinal mixed methods study

Previous reports on the patient perspective of daily life during a 1-year high-grade glioma (HGG) trajectory from the time of diagnosis are sparse. The aim of this longitudinal mixed methods study is to identify the specific needs and preferences for rehabilitation and supportive care and how it links with physical activity, psychological measures and health quality longitudinally over the first year after diagnosis among patients with HGG and their caregivers by integrating qualitative and quantitative findings. Using a longitudinal mixed methods design, patients with malignant glioma (n = 30) and their caregivers (n = 33) were interviewed and completed questionnaires (patients only) about physical activity level, anxiety/depression and quality of life five times during the 1-year period. Their needs and preferences included interventions designed to re-define hope after diagnosis, health promoting physical activities initiated early, psychological symptom management strategies, and life planning. Caregivers are committed to their caregiving role, but their engagement is nonetheless challenged over time by enormous caregiver burdens. The identified specific needs and preferences favour supportive care, education, information and rehabilitation. Guidelines attentive to these needs and implemented in clinical practice have the potential to improve patients' health-related quality of life and support caregivers by involving them more actively in care and management.
Non-normative preaspirated voiceless fricatives in Scottish English: Phonetic and phonological characteristics

Preaspiration is usually associated with stops rather than fricatives, both at phonological and phonetic levels of description. This study reports the occurrence of phonetic (nonnormative)preaspiration of voiceless fricatives in Scottish Standard English (SSE)spoken in the Central Belt of Scotland. We classify it as non-normative because it is variably present in different speakers, but the distribution is nevertheless understandable on phonetic grounds. The paper analyses the phonetic distribution of preaspiration and its functions in SSE. Preaspiration is shown to occur more frequently after open vowels and phrase-finally. Sociophonetic conditioning by speaker’s sex is not found to be relevant. Functional analysis shows that preaspiration (reflected in the amount of noise in mid/high spectralfrequencies) is a systematic correlate of phonological fricative /voice/ contrast phrase finally. In this context, it appears to be even stronger predictor of /±voice/ than such traditionally-considered correlates as voicing offset and segmental duration. The results show that abstract non-neutralised /voice/ is phonetically multidimensional such that fricative preaspiration can maintain the contrast in the contexts where phonetic voicing is demoted. The extent and functioning of preaspiration in SSE suggests that it is a varietyspecific optional characteristic resulting from a learned dissociation of lingual and laryngeal stricture gestures in voiceless fricatives.