Miracle-dz/UniFAR-FTUs huggingface dataset:
Localization and Activity of Myosin Light Chain Kinase Isoforms during the Cell Cycle

Phosphorylation on Ser 19 of the myosin II regulatory light chain by myosin light chain kinase (MLCK) regulates actomyosin contractility in smooth muscle and vertebrate nonmuscle cells. The smooth/nonmuscle MLCK gene locus produces two kinases, a high molecular weight isoform (long MLCK) and a low molecular weight isoform (short MLCK), that are differentially expressed in smooth and nonmuscle tissues. To study the relative localization of the MLCK isoforms in cultured nonmuscle cells and to determine the spatial and temporal dynamics of MLCK localization during mitosis, we constructed green fluorescent protein fusions of the long and short MLCKs. In interphase cells, localization of the long MLCK to stress fibers is mediated by five DXRXXL motifs, which span the junction of the NH2-terminal extension and the short MLCK. In contrast, localization of the long MLCK to the cleavage furrow in dividing cells requires the five DXRXXL motifs as well as additional amino acid sequences present in the NH2-terminal extension. Thus, it appears that nonmuscle cells utilize different mechanisms for targeting the long MLCK to actomyosin structures during interphase and mitosis. Further studies have shown that the long MLCK has twofold lower kinase activity in early mitosis than in interphase or in the early stages of postmitotic spreading. These findings suggest a model in which MLCK and the myosin II phosphatase (Totsukawa, G., Y. Yamakita, S. Yamashiro, H. Hosoya, D.J. Hartshorne, and F. Matsumura. 1999. J. Cell Biol. 144:735–744) act cooperatively to regulate the level of Ser 19–phosphorylated myosin II during mitosis and initiate cytokinesis through the activation of myosin II motor activity.
Linguistic Diversity as Resource: A Multilevel Approach to Building Awareness in First-Year Writing Programs (and Beyond)

Abstract:Drawing on research in systems theory and their own programmatic efforts to recognize, value, and integrate language differences in first-year composition, the authors argue for a multilevel approach for sustainable and systemic change to occur. Multilevel work functions to identify points of leverage for enacting language rights in institutional settings.
FOR ATMOSPHERIC AND ADJACENCY EFFECTS ON HIGH RESOLUTION SENSOR DATA-A CASE STUDY USING IRSP 6 LISS-IV DATA

Path radiance due to aerosols plays an important role over satellite derived reflectance due to higher aerosol optical depth and their seasonal variations. In addition, satellite data from high-resolution sensors are subjected to adjacency effect, which reduces apparent surface contrast by decreasing the top of the atmosphere radiance over bright pixels and increasing the brightness of the dark pixels. In the present study, Second Simulation of Satellite Signal in the solar Spectrum (6S) radiative transfer model was used to analyze influence of atmospheric and adjacency affects over two different study areas corresponds to semi arid and tropical forest regions of India using IRS P6 – LISS-IV satellite data. Ground measurements of surface reflectance over selected crops were conducted to compare satellite derived surface reflectance. 6S model has been calibrated for local conditions using measurements on aerosol optical depth, water vapour and ozone parameters derived from MODIS-TERRA and OMI sensors. The methodology was applied for atmospheric correction of satellite data during intense biomass burning episodes in north-east region of India. Results of the study indicated that satellite reflectance measurements correlated well after correcting the satellite data for atmospheric effects using satellite based atmospheric parameters. Statistical information and seperability analysis over different land use categories suggested improved dynamic range and better seperability after atmospheric corrections. The major outcome of study suggested that atmospheric corrections using satellite derived atmospheric parameters provides a reasonably good data set when ground measurements on atmospheric parameters and surface reflectance are not available.
t-Bu2SiF-Derivatized D2-Receptor Ligands: The First SiFA-Containing Small Molecule Radiotracers for Target-Specific PET-Imaging

The synthesis, radiolabeling and in vitro evaluation of new silicon-fluoride acceptor (SiFA) derivatized D2-receptor ligands is reported. The SiFA-technology simplifies the introduction of fluorine-18 into target specific biomolecules for Positron-Emission-Tomography (PET). However, one of the remaining challenges, especially for small molecules such as receptor-ligands, is the bulkiness of the SiFA-moiety. We therefore synthesized four Fallypride SiFA-conjugates derivatized either directly at the benzoic acid ring system (SiFA-DMFP, SiFA-FP, SiFA-DDMFP) or at the butyl-side chain (SiFA-M-FP) and tested their receptor affinities. We found D2-receptor affinities for all compounds in the nanomolar range (Ki(SiFA-DMFP) = 13.6 nM, Ki(SiFA-FP) = 33.0 nM, Ki(SiFA-DDMFP) = 62.7 nM and Ki(SiFA-M-FP) = 4.21 nM). The radiofluorination showed highest yields when 10 nmol of the precursors were reacted with [18F]fluoride/TBAHCO3 in acetonitrile. After a reversed phased cartridge purification the desired products could be isolated as an injectable solution after only 10 min synthesis time with radiochemical yields (RCY) of more than 40% in the case of SiFA-DMFP resulting in specific activities >41 GBq/µmol (>1,100 Ci/mmol). Furthermore, the radiolabeled products were shown to be stable in the injectable solutions, as well as in human plasma, for at least 90 min.
Optical CDMA Code Collision and Translation Performance Analysis

This paper presents the performance analysis of a code translation (conversion) module for an optical code-division multiple access (O-CDMA) local area network (LAN) system. Simulation results are presented for a code re-using system with two LANs connected by a central router. The same set of codes is being used in each of the two LANs. Code collisions occur at the router when a code in use in one LAN clashes with a message from the other LAN at the same code. A model is developed to simulate the system and to measure the performance characteristics. The simulations indicate that throughput can be increased at least by 20% using a code translation scheme. Furthermore, the network with given number of codes can support twice as many users for the same percentage of code collisions whenever a code translation scheme is employed
Measurement Invariance of the Brief Symptom Inventory-18 (BSI-18) Across Asian American Ethnic, Nativity, and Gender Groups

This study was the first to examine the factor structure, measurement invariance, and criterion-related evidence for the construct validity of the Brief Symptom Inventory-18 (BSI-18; Derogatis, 2000) in an Asian American sample. Multigroup confirmative factor analyses were carried out in a community sample of adults of Asian ethnic descent (N = 624; M = 41.47 years, SD = 13.28) across ethnicity (Chinese, Filipinx, and Vietnamese), nativity status (U.S.-born and Asian-born), and gender (female and male) to test a priori competing models and measurement invariance. Results of bifactor analyses and ancillary estimates provided strong evidence for the general factor of the BSI-18. Invariance tests indicated that BSI-18 items were interpreted in a similar fashion, and responses could be meaningfully compared across ethnicity, gender, and nativity samples. In addition, theory-consistent correlations with self-esteem and racial microaggression experiences provided criterion-related evidence for the BSI-18. Results may not generalize to other Asian American groups (e.g., South Asians, Asian elders, and clinical samples), and researchers are strongly encouraged to conduct further internal structure analyses of the BSI-18 among Asian American populations. In conclusion, we recommend calculation and interpretation of the BSI-18 total score as a measure of general psychological distress for Asian American populations.
Progenitor cells of the olfactory receptor neuron lineage

The olfactory epithelium of the mouse has many properties that make it an ideal system for studying the molecular regulation of neurogenesis. We have used a combination of in vitro and in vivo approaches to identify three distinct stages of neuronal progenitors in the olfactory receptor neuron lineage. The neuronal stem cell, which is ultimately responsible for continual neuron renewal in this system, gives rise to a transit amplifying progenitor identified by its expression of a transcription factor, MASH1. The MASH1‐expressing progenitor gives rise to a second transit amplifying progenitor, the Immediate Neuronal Precursor, which is distinct from the stem cell and MASH1‐expressing progenitor, and gives rise quantitatively to olfactory receptor neurons. Regulation of progenitor cell proliferation and differentiation occurs at each of these three cell stages, and growth factors of the fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) families appear to play particularly important roles in these processes. Analyses of the actions of FGFs and BMPs reveal that negative signaling plays at least as important a role as positive signaling in the regulation of olfactory neurogenesis. Microsc. Res. Tech. 58:176–188, 2002. © 2002 Wiley‐Liss, Inc.
Identification and Classification of Rhizobia by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry

Mass spectrometry (MS) has been widely used for specific, sensitive and rapid analysis of proteins and has shown a high potential for bacterial identification and characterization. Type strains of four species of rhizobia and Escherichia coli DH5α were employed as reference bacteria to optimize various parameters for identification and classification of species of rhizobia by matrix-assisted laser desorption/ionization time-of-flight MS (MALDI TOF MS). The parameters optimized included culture medium states (liquid or solid), bacterial growth phases, colony storage temperature and duration, and protein data processing to enhance the bacterial identification resolution, accuracy and reliability. The medium state had little effects on the mass spectra of protein profiles. A suitable sampling time was between the exponential phase and the stationary phase. Consistent protein mass spectral profiles were observed for E. coli colonies pre-grown for 14 days and rhizobia for 21 days at 4°C or 21°C. A dendrogram of 75 rhizobial strains of 4 genera was constructed based on MALDI TOF mass spectra and the topological patterns agreed well with those in the 16S rDNA phylogenetic tree. The potential of developing a mass spectral database for all rhizobia species was assessed with blind samples. The entire process from sample preparation to accurate identification and classification of species required approximately one hour.
Diagnosis of Liver Involvement in Primary Sjögren Syndrome

Liver involvement was one of the first extraglandular manifestations to be reported in patients with primary Sjögren syndrome (SS). In the 1990s, a study of liver involvement in patients with primary SS integrated the evaluation of clinical signs of liver disease, liver function and a complete panel of autoantibodies. Recent developments in the field of hepatic and viral diseases have significantly changed the diagnostic approach to liver involvement in SS. The most recent studies have shown that, after eliminating hepatotoxic drugs and fatty liver disease, the two main causes of liver disease in primary SS are chronic viral infections and autoimmune liver diseases. The differential diagnosis of liver disease in primary SS (viral vs autoimmune) is clinically important, since the two processes require different therapeutic approaches and have different prognoses. With respect to viral infections, chronic HCV infection is the main cause of liver involvement in SS patients from the Mediterranean area, while chronic HBV infection may be the main cause of liver involvement in SS patients from Asian countries. After eliminating viral hepatitis, primary biliary cirrhosis (PBC) should be considered the main cause of liver disease in primary SS. PBC-related SS patients may have a broad spectrum of abnormalities of the liver, including having no clinical or analytical data suggestive of liver disease. Autoimmune hepatitis (AIH) is the second most frequently found autoimmune liver disease to be associated with SS (all reported cases are type I), and nearly 10% of these patients have an AIH-PBC overlap. Finally, IgG4-related disease must be investigated in patients with SS presenting with sclerosing cholangitis, especially when autoimmune pancreatitis or retroperitoneal fibrosis are also present.
Impact of different walking surfaces on gait identification based on higher-order statistics of accelerometer data

This paper presents an investigation on how different walking surfaces affect gait identification based on accelerometer data. Accelerometer data of 5 subjects walking on 4 different solid surfaces was acquired on 3 different days by a cell phone placed on the subject's hip. Data analysis detects gait cycles by a method based on wavelet transform. For all gait cycles, features were calculated by using higher-order statistics. Similarity estimation for discerning the different subjects and surfaces was introduced by using principal component analysis (PCA). It proved that the different solid surfaces do not influence the efficiency of the identification of subjects based on their gait.
Do cerebral blood volume and contrast transfer coefficient predict prognosis in human glioma?

INTRODUCTION Noninvasive measurements of cerebral blood volume (CBV) and contrast transfer coefficient (K(trans)) have potential benefits in the diagnosis and therapeutic management of adult glioma. This study examines the relationship between CBV, K(trans), and overall survival. METHODS AND MATERIALS Twenty-seven adult patients with glioma underwent T1-weighted dynamic contrast-enhanced MR imaging, and parametric maps of CBV and K(trans) were calculated. The relationship of histologic grade, CBV, K(trans), age, sex, surgical resection, and use of adjuvant therapy to survival were analyzed by using the logrank method and Cox regression analysis. The Kaplan-Meier method for displaying survival curves was used. The relationship of factors such as comorbidity, elevated intracranial pressure, size of nonenhancing tumor, and peritumoral edema were not considered. RESULTS Both CBV (P < .01) and K(trans) (P < .01) show a significant relationship to histologic grade. CBV (P = .004), K(trans) (P = .008), and histologic grade (P < .001) all demonstrate a significant association with patient survival when analyzed individually. Cox regression analysis identified only histologic grade (P < .01) and K(trans) (P < .05) as independent significant prognostic indicators. Examination of survival data from high-grade (III and IV) tumors demonstrated a linear relationship between K(trans) and patient survival (P < .01). CONCLUSION This study suggests a direct relationship between K(trans) and length of survival in high-grade gliomas, which could be of clinical importance. CBV relates directly to histologic grade but provides no independent prognostic information over and above that provided by grade. Further large prospective studies should be planned to test whether this observation holds true.
RuleEd, a Web-based Semantic Network Interface for Constructing and Revising Computable Eligibility Rules

RuleEd is a web-based editing environment which enables clinical trial eligibility rules entered as free text to be represented as a series of terms mapped to unique concepts in a controlled vocabulary. RuleEd provides interfaces for creating and refining concept mappings for terms within rules and disambiguating multiply-mapped terms. A combination of inter-active and non-interactive methods enable authors to specify eligibility rule representations with a fine level of control.
Heterogeneously Catalyzed Fenton-Reversible Addition–Fragmentation Chain Transfer Polymerization in the Presence of Air

Aqueous Fenton-reversible addition–fragmentation chain transfer (RAFT) polymerization catalyzed by heterogeneous catalysts, that is, Fe(II) metal–organic framework (MOF) particles, coupled with hydrogen peroxide (H2O2) with the reaction mixture exposed to air in open vessels is reported. Reactive hydroxyl radicals are generated via a heterogeneous redox reaction between Fe(II) of the MOF particles and H2O2, which then chemically deoxygenate the reaction mixture in situ, initiating RAFT polymerization. Well-controlled polymers (Đ < 1.1) with experimental molecular weights close to theoretical values at high monomer conversions (ca. 85%) were achieved within 15 min. High “livingness” of the synthesized polymer chains was demonstrated by chain extension experiments and matrix-assisted laser desorption/ionization time-of-flight analysis. This study contributes to the growing interest in nonenzymatic deoxygenation techniques via heterogeneous catalysis for conducting radical polymerization reactions.
Why Color Mandalas? A Study of Anxiety-Reducing Mechanisms

Abstract This experimental study examined the validity of 3 underlying mechanisms that have been proposed in the research literature to explain the anxiety-reducing effect of coloring mandalas: distraction, structure, and centering. Four conditions were tested: (a) freely coloring a mandala, (b) coloring a mandala with preset colors, (c) freely drawing a mandala within a preset circle, and (d) coloring a rectangular grid with preset colors. Anxiety was induced in the participants and then measured by self-report and pulse rate. The anxiety-reducing effect of the intervention task was found to be significant in all 3 mandala conditions as compared to the control condition. The centering explanation was supported by the physiological data but not the self-reported data. The structure explanation was not supported by either source of data. The distraction explanation could not be verified because the manipulation of distraction was not successful. Findings from this study support the use of mandalas as an anxiety-reduction tool in art therapy, however the validity of the distraction effect needs further investigation.
A novel approach for characterizing the mixing zone of a saltwater wedge

The mixing zone between freshwater and seawater in a coastal aquifer is an important transition region that controls regional groundwater flow dynamics and reactive transport processes. While experimental procedures have been developed to study the movement of salt wedges in aquifers, so far no one has attempted to exclusively map the mixing zone associated with the wedge in a laboratory‐scale aquifer model. We propose a colorimetric experimental method, which employs the reaction between alkaline freshwater and acidic saltwater, to map the mixing zone. The experiment was completed in a two‐dimensional aquifer model to simulate a steady saltwater wedge. We used the density‐dependent flow model SUTRA to simulate these experimental results and to estimate the mixing parameters, i.e., dispersivity coefficients. The results show that the proposed colorimetric technique is a useful experimental approach to visualize and quantify the level of mixing in a saltwater intrusion experiment.
Crossing Boundaries: A Case Study of Employee Blogging

Editors, email, and instant messaging were first widely used by students who later brought knowledge of their uses and effective practices into workplaces. Weblogs may make such a transition more quickly. We present a study of emergent blogging practices in a corporate setting. We attended meetings, read email, documents, and Weblogs, and interviewed 38 people loggers, infrastructure administrators, attorneys, public relations specialists, and executives. We found an experimental, rapidly-evolving terrain marked by growing sophistication about balancing personal, team, and corporate incentives and issues
Analysis and Design of Warning Delivery Service in Intervehicular Networks

This paper focuses on inter-vehicular networks providing warning delivery service. As soon as a danger is detected, the propagation of a warning message is triggered, with the aim of guaranteeing a safety area around the point in which the danger is located. Multiple broadcast cycles can be generated so that a given lifetime of the safety area is guaranteed. The service is based on multi-hop ad hoc inter-vehicular communications with a probabilistic choice of the relay nodes. The scenario we consider consists of high speed streets, such as highways, in which vehicles exhibit one-dimensional movements along the direction of the road. We propose an analytical model for the study of this service and derive performance indices such as the probability that a vehicle is informed, the average number of duplicate messages received by a vehicle and the average delay. Moreover, we use the model to discuss system design issues, which include the proper setting of the forwarding probability at each vehicle, so that a given probability to receive the warning can be guaranteed to all vehicles in the safety area. The model is validated against simulation results. Since it is very accurate, the model can be instrumental to the performance evaluation and design of broadcasting techniques in inter-vehicular networks.
Clusters and groups of galaxies in the 2dF galaxy redshift survey: A new catalogue

We create a new catalogue of groups and clusters, applying the friends-of-friends method to the 2dF GRS final release. We investigate various selection effects due to the use of a magnitude limited sample. For this purpose we follow the changes in group sizes and mean galaxy number densities within groups when shifting nearby observed groups to larger distances. We study the distribution of sizes of dark matter haloes in N -body simulations and compare properties of these haloes and the 2dF groups. We show that at large distances from the observer luminous and intrinsically greater groups dominate, but in these groups only very bright members are seen, which form compact cores of the groups. These two effects almost cancel each other, so that the mean sizes and densities of groups do not change considerably with distance. Our final sample contains 10750 groups in the Northern part, and 14465 groups in the Southern part of the 2dF survey with membership Ngal ≥ 2. We estimate the total luminosities of our groups, correcting for group members fainter than the observational limit of the survey. The cluster catalogue is available at our web-site (http://www.aai.ee/∼maret/2dfgr.html). (© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Mirror symmetry for orbifold Hurwitz numbers

We study mirror symmetry for orbifold Hurwitz numbers. We show that the Laplace transform of orbifold Hurwitz numbers satisfy a dierential recursion, which is then proved to be equivalent to the integral recursion of Eynard and Orantin with spectral curve given by the r-Lambert curve. We argue that the r-Lambert curve also arises in the innite framing limit of orbifold Gromov-Witten theory of (C 3 =(Z=rZ)). Finally, we prove that the mirror model to orbifold Hurwitz numbers admits a quantum curve.