Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional Studies of the Yeast Med5, Med15 and Med16 Mediator Tail Subunits

The yeast Mediator complex can be divided into three modules, designated Head, Middle and Tail. Tail comprises the Med2, Med3, Med5, Med15 and Med16 protein subunits, which are all encoded by genes that are individually non-essential for viability. In cells lacking Med16, Tail is displaced from Head and Middle. However, inactivation of MED5/MED15 and MED15/MED16 are synthetically lethal, indicating that Tail performs essential functions as a separate complex even when it is not bound to Middle and Head. We have used the N-Degron method to create temperature-sensitive (ts) mutants in the Mediator tail subunits Med5, Med15 and Med16 to study the immediate effects on global gene expression when each subunit is individually inactivated, and when Med5/15 or Med15/16 are inactivated together. We identify 25 genes in each double mutant that show a significant change in expression when compared to the corresponding single mutants and to the wild type strain. Importantly, 13 of the 25 identified genes are common for both double mutants. We also find that all strains in which MED15 is inactivated show down-regulation of genes that have been identified as targets for the Ace2 transcriptional activator protein, which is important for progression through the G1 phase of the cell cycle. Supporting this observation, we demonstrate that loss of Med15 leads to a G1 arrest phenotype. Collectively, these findings provide insight into the function of the Mediator Tail module.
A Perspective of Theory of Planned Behavior and Attribution Theory for PBB P2 Taxpayer Compliance in Probolinggo

This study aims to show that attitude to comply, subjective norms, and perceived behavioral control had affect the intent to obey. Furthermore, perceived behavioral control and intention to abide affect on PBB tax compliance refers to the Theory of Planned Behavior. This study also adds a variable: the sanctions, that refers to Attribution Theory which predicted to affect PBB taxpayer compliance behavior. The sample was PBB taxpayers who have tax object in Probolinggo. The results showed that the attitude to obey had positive influence on the intention to comply, subjective norms had negatively affected on the intention to obey and perceived behavioral control had positive influence on the intention to obey. Moreover, these results also prove empirically that the intention to obey and perceived behavioral control had positive effect on PBB tax compliance, while the sanctions have no effect on PBB tax compliance. In addition, the intention to obey have the effect of partial mediation on relationships perceived behavioral control and PBB taxpayer compliance.
Interest Rate Smoothing and Time-Varying Premium: Another Look at Debt Management in Japan

We argue a source of time-varying premium (TVTP) in Japanese government bond market, and show that it is interest rate smoothing that causes empirical failures of expectation theory of term structure of interest rates. We estimate a regime switching ARCH model where an interest rate smoothing regime can be identified. Based on a model of time-inconsistency by Missale and Blanchard (1994), we further focus on a role of debt maturity in TVTP, which is an alternative to an ARCH process. Our robust empirical evidences support the expectation theory in Japanese government bond market. Moreover, in comparison with the ARCH process, debt maturity turns out to be a reliable proxy for the TVTP. This shows a possibility of a debt management policy in Japan: fiscal authority takes advantage of the debt maturity for price stability which is a target of monetary policy. It sharply contrasts with an evidence for ineffectiveness of the U.S. debt management policy by Wallace and Warner (1996).
Study on Preliminary Performance of Algorithms for Network Traffic Identification

At present, more and more scholars paid attention to accuracy rate of Internet traffic identification, but not on construction model time, test time, CPU utilization, memory consumption, and concision of models description between different algorithms. However, these indicators played a decisive role in performance of practical internet traffic identification system. So, we collected traffic from existing operatorspsila networks, and tested 15 kinds of supervised learning algorithms and so on. The multivariate evaluation method was proposed to assess the test results. The results show that the 15 kinds of algorithms have similar accuracy rate, but their construction model time, test time, and concision of models description are very different. The C4.5 algorithm is the most suitable classification algorithms for network traffic identification.
Effects of Skim Milk Powder Supplementation to Soy Yogurts on Biotransformation of Isoflavone Glycosides to Biologically Active Forms during Storage

Three batches of yogurts were made with soy protein isolate (SPI) supplemented with 2% (S2), 4% (S4) or 6% (S6) of skim milk powder (SMP). The fourth batch (control; S0) was prepared from SPI without SMP supplementation. Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842 (Lb 11842) and Streptococcus thermophilus ST 1342 (ST 1342) were used as the starter culture. Biotransformation of the inactive forms, isoflavone glycosides (IG) to biologically active forms, isoflavone aglycones (IA), was determined during 28 d storage. The viability of both microorganisms was significantly higher (P < 0.05) in S2, S4, and S6 than that in S0. The ratio of lactic acid/acetic acid in S0 was in the range of 15.53 - 22.31 compared to 7.24 - 12.81 in S2, S4 and S6. The biotransformation of IG to IA in S2, S4 and S6 was also enhanced by 9.9 -13.3% compared to S0.
Does Psi Exist? Reply to Storm and Ertel (2001)

The authors recently published a nonsignificant meta-analysis of 30 extrasensory perception ganzfeld studies, all conducted after the 1986 publication of important methodological guidelines aimed at reducing sources of artifact noted in earlier studies. In response, L. Storm and S. Ertel (2001) presented a meta-analysis of 79 studies published between 1974 and 1996. They argued that the positive and highly statistically significant overall outcome indicates a replicable paranormal effect. In doing so, they ignored the well-documented and widely recognized methodological problems in the early studies, which make it impossible to interpret the results as evidence of extrasensory perception. In addition, Storm and Ertel's meta-analysis is not an accurate quantitative summary of ganzfeld research because of methodologica l problems such as their use of an inconsistent method for calculating study outcomes and inconsistent inclusion criteria. Storm and Ertel (2001) presented a meta-analysis of ganzfeld studies, which, they argued, provides strong evidence of a replicable and genuine communication anomaly. We disagree both with their conclusion and their view of the role of methodologica l quality in attempts to resolve whether extrasensory perception exists. In this article, we discuss the problems in the early ganzfeld studies that make it difficult to draw strong conclusions from meta-analyses such as Storm and Ertel's that include them, additional problems in Storm and Ertel's meta-analytic approach, the general difficulties in using meta-analysis to identify and correct for methodological problems in constituent studies, and a possible approach to the replication problem within parapsychology.
You don’t see the world through the same eyes any more: The impact of sexual offending work on police staff

This paper examines the experiences of police staff in England who work with sexual offence material (SOM). Eleven officers completed a questionnaire then took part in semi-structured interviews. The data were analysed in two stages: Interpretative Phenomenological Analysis was used to illuminate the ‘lived experience’ of participants, and establish a theme structure. Clinical models of workplace trauma were then employed to explore the theme ‘Impact of working with sexual offending’. Impact includes cognitive intrusions and increased suspiciousness. The authors identify where officers’ accounts intersect with nascent symptoms of both Vicarious Traumatisation (McCann and Pearlman, 1990) and post-traumatic stress disorder (PTSD).
Cone Contacts, Mosaics, and Territories of Bipolar Cells in the Mouse Retina

We report a quantitative analysis of the different bipolar cell types of the mouse retina. They were identified in wild-type mice by specific antibodies or in transgenic mouse lines by specific expression of green fluorescent protein or Clomeleon. The bipolar cell densities, their cone contacts, their dendritic coverage, and their axonal tiling were measured in retinal whole mounts. The results show that each and all cones are contacted by at least one member of any given type of bipolar cell (not considering genuine blue cones). Consequently, each cone feeds its light signals into a minimum of 10 different bipolar cells. Parallel processing of an image projected onto the retina, therefore, starts at the first synapse of the retina, the cone pedicle. The quantitative analysis suggests that our proposed catalog of 11 cone bipolar cells and one rod bipolar cell is complete, and all major bipolar cell types of the mouse retina appear to have been discovered.
Goal Difficulty and Creative Performance: The Mediating Role of Stress Appraisal

ABSTRACT We investigated whether the effect of goal difficulty on creativity may be clarified by examining how people appraise their working conditions. In an experiment, 157 undergraduate students completed a divergent thinking task for which goal difficulty was manipulated. Stress appraisals were assessed through self-reports, and creativity was evaluated by independent judges. Results indicated that difficult goals, although categorized as a challenge stressor, can also be appraised as a hindrance and/or a threat. Although there was a direct positive effect of difficult goals on creative performance, hindrance appraisals partially mediated a negative relationship between goal difficulty and creative performance. Results highlight that stress appraisals can help clarify inconsistent effects of stressors, with implications for managing creativity.
Digitalisation in the banking industry and workers subjective well-being

Purpose The purpose of this paper is to explore how bankers perceive digitalisation relating to their subjective well-being. The paper seeks to further explore how this relation is contingent on the aspect of structural organisation represented by the concept of individualist/collectivist organisational culture. Design/methodology/approach The study is based on the survey distributed to employees of 18 bank offices in the south of Sweden, which 161 employees answered. The analysis of the data was performed by descriptive statistics, principle component analysis, Pearson correlations, multiple linear and moderating multiple linear regression analyses. Findings The study indicates that bankers’ experience digitalisation as a four-faceted construct: a tool for information management, and work optimisation, customer relation management and as a change agent. The study suggests that the use of digital tools for work optimisation has a positive relation to the work- related dimensions of subjective well-being as well as a spillover effect on the life balance and life satisfaction dimensions. It also indicates that the information management dimension has a positive relation to the life satisfaction aspect of subjective well-being. Finally, the study found that increasing the degree of collectivist organisation culture has a positive moderating effect on the relation between the use of digital tools for work optimisation and life balance and subjective well-being, respectively. Originality/value The study reveals a new way of operationalising digitalisation in banks and is the first study of its type to explore the relationship between digitalisation different facets and banker subjective well-being.
Sex Differences in Video Game Play:

In this study, we examined gender differences in video game use by focusing on interpersonal needs for inclusion, affection, and control, as well as socially constructed perceptions of gendered game play. Results of a large-scale survey (n = 534) of young adults’ reasons for video game use, preferred game genres, and amount of game play are reported. Female respondents report less frequent play, less motivation to play in social situations, and less orientation to game genres featuring competition and three-dimensional rotation. Implications for game design are discussed.
D = 2+1 N = 2 Yang-Mills theory from wrapped branes

We find a new solution of type-IIB supergravity which represents a collection of D5-branes wrapped on the topologically non-trivial S^3 of the deformed conifold geometry T*S^3. The type-IIB solution is obtained by lifting a new solution of D = 7 SU(2)_L × SU(2)_R gauged supergravity to ten dimensions in which SU(2)_D gauge fields in the diagonal subgroup are turned on. The supergravity solution describes a slice of the Coulomb branch in the large-N limit of
Technology Adoption and Absorption : The Case of China

Using a unique firm-level survey dataset collected by one of the authors, this paper addresses two questions central to the literature on technology diffusion: What factors influence technology adoption, and how well do firms absorb newly acquired technology? We find that given the same technical capacity, firms with better access to cheap bank credit are more likely to adopt larger technology projects and invest more in imported equipment from technologically-advanced countries. On the other hand, the return to technology investment differs significantly across firms. In particular, firms with better access to cheap credit have significantly lower project profitability and capacity utilization. These results have important implications for the role of financial development in technology diffusion. (JEL Classification: O3) ∗Brandt: Department of Economics, University of Toronto, 150 St. George Street, Toronto, Ontario, M5S 3G7, Canada. Zhu: Department of Economics, Michigan State University, Marshall Hall, East Lansing, MI, 48824, USA. We are grateful to Steven Haider, Bart Hobijn, Rich Jensen, Raoul Minetti, Ximing Wu, seminar participants at McGill University, Michigan State University, and conference participants in the Mid-West International Economics Meeting 2002, the 9th EIIT conference, the Econometric Society North American Summer Meeting 2003, the NBER Summer Institute 2003, and the CEA Annual Meetings 2004. Brandt would like to acknowledge the support of the Shanghai Economic Research Center and the National Statistical Bureau for their help in carrying out the survey. He would also like to thank former colleague Yehuda Kotowitz for his important contribution to the project at an earlier date. Financial support for the survey was provided by the Canadian International Development Agency.
Preparing Teacher Trainees for Field Experience: Lessons From the On-Campus Practical Experience in Colleges of Education in Ghana

This study sought to understand the process of providing on-campus practical experience to teacher trainees in preparation for practicum in basic schools, and how the trainees perceive these. It used the mixed method approach to obtain data from three purposively sampled colleges of education in the Central Region of Ghana. Questionnaires were administered to 232 teacher trainees; 12 focus group discussion sessions were held with the trainees while an interview guide was used to elicit data from 24 college tutors. The findings showed that the on-campus experience offered trainees the opportunity to improve on their knowledge and practice of general pedagogical skills such as the writing of lesson plans, statement of appropriate lesson objectives, lesson delivery, timing of activities, and the use of teaching and learning resources. However, there was no evidence of documented standards and guidelines for the conduct of the on-campus practicum. Again, trainees had very limited time to engage with supervisors and critically interrogate and reflect on their own practices. Consequently, it was suggested that teacher training institutions should develop appropriate teaching standards and guidelines for on-campus teaching practice and give trainees more opportunity to engage with and critically interrogate their own practice in the process of learning to teach.
The origins of narcissism and narcissistic personality disorder: A theoretical and empirical reformulation.

lthough they are concepts with long psychoanalytic pedigrees, A narcissism and narcissistic personality disorder only recently have become topics of concern not just for psychoanalysis but for empirical research in psychology and psychiatry as well. For the most part, research in this area has comprised essentially descriptive or atheoretical attempts to identify and measure these constructs,' and only a few Portions of this chapter were presented to the Psychology Service of the Bronx Veterans Affairs Medical Center. I thank Deborah Bryan and Joseph Masling for their many comments, both editorial and substantive, on this manuscript.
Analysis of Political Language Based on Appraisal Theory: The Mutual Construction of Language and Power—Taking Xi Jinping and Donald Trump’s Speeches at World Economic Forum as Examples

Davos Forum, also known as World Economic Forum, is a non-official international organization aiming at discussing problems in world economy and promoting international economic cooperation and communication. Based on James R. Martin’s Appraisal Theory, this paper attempts to make a contrastive analysis between Xi Jinping’s and Donald Trump’s speeches at World Economic Forum in 2017 and 2018 respectively. The purpose is to explore how they convey ideology in their speeches, how they make use of language resources to coordinate emotions, judgments and appreciation, as well as how they engage and graduate semantic contents, thereby exploring the mutual construction of language and power. Keywords—Appraisal theory; World Economic Forum; Attitude; Engagement; Graduation
Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products

ABSTRACT To further explore possible avenues for accessing microbial biodiversity for drug discovery from natural products, we constructed and screened a 5,000-clone “shotgun” environmental DNA library by using an Escherichia coli-Streptomyces lividans shuttle cosmid vector and DNA inserts from microbes derived directly (without cultivation) from soil. The library was analyzed by several means to assess diversity, genetic content, and expression of heterologous genes in both expression hosts. We found that the phylogenetic content of the DNA library was extremely diverse, representing mostly microorganisms that have not been described previously. The library was screened by PCR for sequences similar to parts of type I polyketide synthase genes and tested for the expression of new molecules by screening of live colonies and cell extracts. The results revealed new polyketide synthase genes in at least eight clones. In addition, at least five additional clones were confirmed by high-pressure liquid chromatography analysis and/or biological activity to produce heterologous molecules. These data reinforce the idea that exploiting previously unknown or uncultivated microorganisms for the discovery of novel natural products has potential value and, most importantly, suggest a strategy for developing this technology into a realistic and effective drug discovery tool.
Oceanic nutrient and oxygen transports and bounds on export production during the World Ocean Circulation Experiment

Large‐scale oceanic transports of nutrient and oxygen are estimated from selected hydrographic sections from the World Ocean Circulation Experiment spanning the world ocean. A so‐called geostrophic inverse box model is used to calculate the velocity field across the transoceanic sections. The circulation is required, a priori, to conserve mass, salt, top‐to‐bottom silicate, and subsurface heat and PO( = 170P + O2). The resulting estimate of the time mean circulation is used to compute horizontal and vertical nutrient transports and their residual sources and sinks associated with biogeochemical processes. Locally, the remineralization rate is, in general, below our uncertainty level, with magnitudes consistent with in situ measurements (0 ± 0.1mol N m−2 yr−1 to 0.7 ± 0.25 mol N m−2 yr−1 below about 100 m). Because of correlations between errors, the export production becomes significant when integrated globally, with 390 ± 240 kmol Si s−1 and 57 ± 40 kmol N s−1(420 ± 290 kmol C s−1) between 47°N and 30°S and at 2000 m. Examination of N* provides estimates for nitrate fixation and denitrification, either consistent (North Atlantic) or contradictory (Indian) with previous findings. Similarily, oxygen utilization rates and net air‐sea exchanges are provided. A net uptake of oxygen at high latitudes and an outgassing at low latitudes are found. Examination of the pseudotracer O* suggests an important role of dissolved organic matter in the oxygen budget. Oceanic oxygen and O* are found in global balance within uncertainties.
Swift XRT Observations of the Afterglow of XRF 050416A

Swift discovered XRF 050416A with the Burst Alert Telescope and began observing it with its narrow-field instruments only 64.5 s after the burst onset. Its very soft spectrum classifies this event as an X-ray flash. The afterglow X-ray emission was monitored up to 74 days after the burst. The X-ray light curve initially decays very fast (decay slope α ~ 2.4), subsequently flattens (α ~ 0.44), and eventually steepens again (α ~ 0.88), similar to many X-ray afterglows. The first and second phases end ~172 and ~1450 s after the burst onset, respectively. We find evidence of spectral evolution from a softer emission with photon index Γ ~ 3.0 during the initial steep decay, to a harder emission with Γ ~ 2.0 during the following evolutionary phases. The spectra show intrinsic absorption in the host galaxy with column density of ~6.8 × 1021 cm-2. The consistency of the initial photon index with the high-energy BAT photon index suggests that the initial fast decaying phase of the X-ray light curve may be the low-energy tail of the prompt emission. The lack of jet break signatures in the X-ray afterglow light curve is not consistent with empirical relations between the source rest-frame peak energy and the collimation-corrected energy of the burst. The standard uniform jet model can give a possible description of the XRF 050416A X-ray afterglow for an opening angle larger than a few tens of degrees, although numerical simulations show that the late-time decay is slightly flatter than expected from on-axis viewing of a uniform jet. A structured Gaussian-type jet model with uniform Lorentz factor distribution and viewing angle outside the Gaussian core is another possibility, although a full agreement with data is not achieved with the numerical models explored.