Miracle-dz/UniFAR-FTUs huggingface dataset:
Co-Regulation of Cell Polarization and Migration by Caveolar Proteins PTRF/Cavin-1 and Caveolin-1

Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration.
RATIONALE FOR SPINAL ANESTHESIA

Spinal anesthesia is probably the most widely used form of regional anesthesia today. A number of clinical studies suggest that spinal anesthesia may be superior to general or epidural anesthesia for certain patients and for certain surgical procedures. The endocrine-metabolic response to surgery appears to be blunted when spinal anesthesia is employed compared to the response during general anesthesia. Blood loss and thromboembolic complications are also reduced when spinal anesthesia is used, particularly for major lower limb orthopedic procedures. Although long-term survival is not affected by the anesthetic technique employed, short-term mortality may be decreased in elderly patients having major orthopedic surgery under spinal anesthesia. Compared to epidural blockade, spinal anesthesia provides a more rapid onset, a more predictable level of analgesia, and a more profound degree of surgical anesthesia. On the other hand, spinal anesthesia is associated with a greater degree of hypotension compared to epidural anesthesia. The results of these various investigations show that a scientific rationale exists for the continued use of spinal anesthesia.
Local governance and business performance in Vietnam: the transaction costs’ perspective

ABSTRACT Local governance and business performance in Vietnam: the transaction costs’ perspective. Regional Studies. This paper adopts a transaction costs’ perspective to explain why the growth of small and medium-sized enterprises (SMEs) may vary across regions of an emerging economy. Furthermore, it is argued that young and small firms gain more from the improvement of local governance than do old and large firms. In addition, depending on the institutional history, SMEs will respond differently to the incentives provided by local governance. Analysing more than 300,000 SMEs in Vietnam during 2006–12, it is shown that higher-quality local governance positively influences local SME revenue growth; this effect is stronger for young and small firms, and matters more where institutional history suggests there is less support for entrepreneurship.
The Effectiveness of Individual Psychoeducation on Functioning and Quality of Life with Bipolar Disorder in Turkey : A Randomized Controlled Study

Background: With psycho-education programs, patients diagnosed with bipolar disorder can increase the functionality and quality of their lives. Objective: To determine the effectiveness of the four-session individual psycho-education program designed to improve functionality and quality of life. Method: This research was conducted as a randomized, contr olled, experimental study. Eighty-two patients diagnosed with bipolar disorder participated and we re assigned to intervention and control groups. Results: Socio-demographic and the main clinical features su ch as mean number of total episodes ( T = 2.139; P = 0.036) were equal across the intervention and control gr ups. Comparing patients’ functionality level score s, a statistically significant difference ( T = 2.311; P = 0.024) was found between groups in the “ motional functionality” subscale 6 months after psycho-education ( T = 2.311; P = 0.024). Another significant difference was determined in the “ participation in social activities” subscale after 6 months, ( T = 2.011; P = 0.048), and again at the 12th month ( T = 2.674; P = 0.009). Another significant difference was found in the “ taking initiative” subscale before psycho-education ( T =2.093; P = 0.040). Examining quality of life, a statistically signific ant difference was found only in the “ nvironmental quality of life” subscale before psycho-education ( T = 3.762; P =0.000). Conclusions: Four-session individual psycho-education increases th rate of participation in social activities; however, individual psycho-education seems to be in effective for improving other functioning and overa ll quality of life.
Acquired von Willebrand syndrome and mitral valve prosthesis leakage. A pilot study

Background: Of patients with severe aortic stenosis, 15–25% present with bleeding episodes possibly attributable to acquired von Willebrand syndrome (AVWS). AVWS associated with mitral valve prosthesis leakage has not been reported. Methods and Results: Five patients receiving appropriate oral anticoagulation showed mitral valve prosthesis leakage and bleeding episodes; all of them required hospitalization and two blood transfusions, and a von Willebrand factor (VWF) analysis was performed. Two patients with normal functioning metallic prosthesis valves were included as controls. Before surgery, after cessation of acenocumarol, the patients had prolonged activated partial thromboplastin time; four had prolonged closure time (CT) from the platelet function analyzer. Factor VIII procoagulant activity (FVIII:C), VWF ristocetin cofactor activity (VWF:RCo), and VWF collagen binding (VWF:CB) were considerably elevated, while VWF antigen (VWF:Ag) was most elevated. Disproportionate VWF:RCo/VWF:Ag and VWF:CB/VWF:Ag ratios were seen with the loss of large VWF multimers. Following surgery, all parameters were markedly increased and the ratios, CT, and multimeric VWF profile became normal. Conclusions: Acquired VWF qualitative alterations in mitral valve prosthesis leakage may be associated with or contribute to bleeding diathesis. AVWS should be taken into consideration in patients with mitral valve prosthesis leakage with bleeding diathesis not explained by excessive oral anticoagulation.
Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

ABSTRACT We report here four cases of continuous ambulatory peritoneal dialysis-related peritonitis caused by three different species of Gordonia. The portal of entry was likely through Tenckhoff catheters. 16S rRNA and secA1 gene sequencing are so far the most reliable methods for the accurate identification of Gordonia species.
Impact of diabetes mellitus on complications and outcomes of adjuvant chemotherapy in older patients with breast cancer.

PURPOSE To evaluate whether diabetes affects patterns of adjuvant chemotherapy use, toxic effects of chemotherapy, and breast cancer outcomes. PATIENTS AND METHODS By using the Surveillance, Epidemiology, and End Results-Medicare database, we identified patients aged 66 years or older who had stages I through III breast cancer that was diagnosed between 1992 and 2002. Multivariable regression analyses were performed to determine the effect of diabetes on use of chemotherapy, toxicities, and outcomes. The risks of all-cause mortality and breast cancer-specific (BCS) mortality were estimated with the Kaplan-Meier method. RESULTS Our cohort had 70,781 men and women, of whom 14,414 (20.36%) had diabetes. Among people who received chemotherapy (n = 11,826), 21.0% were diabetics. In this group, diabetics had lower odds of receiving anthracyclines (odds ratio [OR], 0.78; 95% CI, 0.71 to 0.87) and taxanes (OR, 0.86; 95% CI, 0.75 to 0.99). Diabetes was associated with increased odds of being hospitalized for any chemotherapy toxicity (OR, 1.38; 95% CI, 1.23 to 1.56), for infection or fever (OR, 1.43; 95% CI, 1.2 to 1.7), for neutropenia (OR, 1.22; 95% CI, 1.03 to 1.45), for anemia (OR, 1.24; 95% CI, 1.05 to 1.47), and for any cause (OR, 1.32; 95% CI, 1.19 to 1.46). Patients with diabetes had higher all-cause mortality (hazard ratio [HR], 1.35; 95% CI, 1.31 to 1.39). There was a significant interaction between diabetes and chemotherapy use for BCS mortality. Diabetic and nondiabetic patients who did not receive chemotherapy had similar BCS mortality, but diabetic patients who did receive chemotherapy had higher BCS mortality than nondiabetic patients (OR, 1.20; 95% CI, 1.07 to 1.35). CONCLUSION In this observational, hypothesis-generating study, patients who have breast cancer and diabetes are at increased risk of chemotherapy-related toxicities compared with nondiabetic patients who are receiving chemotherapy and have higher all-cause mortality.
Econometric estimation of scale and scope economies within the Port Sector: a review

Seaports provide multiple services to ships, cargo and passengers. The size of the port and type of service they offer are two key elements when deciding whether competition is feasible and how to promote it or, conversely, regulation is needed. Analysing this requires a profound knowledge of the cost structure of the activity involved. This means not only knowing total costs for different volumes of aggregated traffic, but also the behaviour of costs when part of the bundle is produced, i.e. when the mix changes. The optimal organization of the industry can be studied by means of cost and production functions. This paper offers a review of the relatively scarce literature about econometric ports’ cost structure, and highlights the role of the multioutput approach as the correct one because it allows the calculation of key cost indicators (economies of scale, scope and so forth) to determine the optimal port industrial structure for a given forecast of demand (traffic mix and volume).
Preventive Therapy of Mental Disorders

According to the World Health Organization, 1 in every 4 people develops a mental disorder. However, despite the huge efforts in the field, our knowledge about the basic mechanisms of mental disorders is still limited. Although psychiatric medications have been widely prescribed for the past decade, the low efficacy of the treatments, the lack of new validated targets or absence of robust animal models are some critical factors among the others that point out to the urgent need to create a new “culture for accelerating” translational medicine [1].
Audio vs. Chat: The Effects of Group Size on Media Choice

The increasing usage of audio and chat communication in private and commercial cooperative settings requires new insight into choosing the appropriate media for collaborative tasks. The paper presents the results of two series of experiments comparing audio and chat communication with varying group sizes. The experimental data indicates that chat scales up better to an increase in group size than audio. We propose that the media richness theory appropriately predicts the productivity of small groups, while the media characteristics proposed by the theory of media synchronicity as well as media speed can be used to predict larger group productivity
Economic growth and institutions in developing countries: Panel evidence

Numerous empirical studies have documented the evidence of institutional significance towards economic growth. This study extends such evidence as it examines the link between institutions and growth in developing countries including East Asian region. By using neoclassical growth framework augmented with institutional controls and latest estimation technique in panel data analysis, this study finds evidence of positive institutions growth-effects and uncovers the channel of their effects toward growth. This study also fills the gap in the East Asian growth literature, in which, to the best of our knowledge, only two studies namely Rodrik (1997) and Campos and Nugent (1999) that document the institutional importance toward economic growth for the region and apparently these studies are for the period before the 1997 Asian Financial Crisis.
Improving quality of care for patients with ovarian and endometrial cancer

Aim Recent studies have shown that 7-12% of endometrial cancers (ECs) are ultramutated due to somatic mutation in the proofreading exonuclease domain of the DNA replicase POLE. Interestingly, these tumors have an excellent prognosis. In view of the emerging data linking mutation burden, immune response and clinical outcome in cancer, we investigated whether POLE-mutant ECs showed evidence of increased immunogenicity. Methods We examined immune infiltration and activation according to tumor POLE proofreading mutation in a molecularly defined EC cohort including 47 POLE-mutant tumors. We sought to confirm our results by analysis of RNAseq data from the TCGA EC series and used the same series to examine whether differences in immune infiltration could be explained by an enrichment of immunogenic neoepitopes in POLE-mutant ECs. Results Compared to other ECs, POLE-mutants displayed an enhanced cytotoxic T cell response, evidenced by increased numbers of CD8+ tumor infiltrating lymphocytes and CD8A expression, enrichment for a tumor-infiltrating T cell gene signature, and strong upregulation of the T cell cytotoxic differentiation and effector markers T-bet, Eomes, IFNG, PRF and granzyme B. This was accompanied by upregulation of T cell exhaustion markers, consistent with chronic antigen exposure. In-silico analysis confirmed that POLE-mutant cancers are predicted to display more antigenic neoepitopes than other ECs, providing a potential explanation for our findings. Conclusions Ultramutated POLE proofreading-mutant ECs are characterized by a robust intratumoral T cell response, which correlates with, and may be caused by an enrichment of antigenic neo-peptides. Our study provides a plausible mechanism for the excellent prognosis of these cancers.
Carcass and meat characterization of “ yacare overo ” ( Caiman latirostris ) and “ yacare negro ” ( Caiman yacare )

Ten caimans (Caiman latirostris and Caiman yacare) of different length and live weight were slaughtered to assess carcass yield and meat quality traits. Carcass yield was 54% while meat portion was the 62%. The carcass meat/bone ratio was approximately 1,51 while the 6,4% corresponded to the fat depots, mostly visceral depots. The tail cut represented the 27,4% of the carcass weight and it was composed 21,9% by meat and the 5,5% of bone. The meat tail pH postsmortem value (6,88 ±0,22) fell up to 6,49 (±0,23), 24 hs after slaughtering and 5,85 (±0,12) after thawing. Cooking losses were low (<0,3%) and the Warner Bratzler shear force had values lower than 3kg. The raw tail color analyses characterized the meat as luminous (L*= 67,7) and pale (C*= 5,5). While the fat content changed significantly depending on the live weight (2,5-29,8%DM), the protein content was relatively constant and around the 65% of dry matter. From the total intramuscular fatty acid content of tail meat, the 41,4 % were satured, the 39,1% were monounsatured and the 10,7% were polyunsatured, with a n-6/n-3 ratio near the optimum (3,16 vs 4). The oleic acid was the most important FA followed by the palmitic, stearic and linoleic acids. Among the unsatured FA, the levels of essential FA as araquidonic (4,34) and n-3 derived family were important (EPA= 0,76 and DHA= 0,57). Another positive nutritional characteristic was the high level of CLA (1,87%FAtot).
Analysis of the Transcriptome of Erigeron breviscapus Uncovers Putative Scutellarin and Chlorogenic Acids Biosynthetic Genes and Genetic Markers

Background Erigeron breviscapus (Vant.) Hand-Mazz. is a famous medicinal plant. Scutellarin and chlorogenic acids are the primary active components in this herb. However, the mechanisms of biosynthesis and regulation for scutellarin and chlorogenic acids in E. breviscapus are considerably unknown. In addition, genomic information of this herb is also unavailable. Principal Findings Using Illumina sequencing on GAIIx platform, a total of 64,605,972 raw sequencing reads were generated and assembled into 73,092 non-redundant unigenes. Among them, 44,855 unigenes (61.37%) were annotated in the public databases Nr, Swiss-Prot, KEGG, and COG. The transcripts encoding the known enzymes involved in flavonoids and in chlorogenic acids biosynthesis were discovered in the Illumina dataset. Three candidate cytochrome P450 genes were discovered which might encode flavone 6-hydroase converting apigenin to scutellarein. Furthermore, 4 unigenes encoding the homologues of maize P1 (R2R3-MYB transcription factors) were defined, which might regulate the biosynthesis of scutellarin. Additionally, a total of 11,077 simple sequence repeat (SSR) were identified from 9,255 unigenes. Of SSRs, tri-nucleotide motifs were the most abundant motif. Thirty-six primer pairs for SSRs were randomly selected for validation of the amplification and polymorphism. The result revealed that 34 (94.40%) primer pairs were successfully amplified and 19 (52.78%) primer pairs exhibited polymorphisms. Conclusion Using next generation sequencing (NGS) technology, this study firstly provides abundant genomic data for E. breviscapus. The candidate genes involved in the biosynthesis and transcriptional regulation of scutellarin and chlorogenic acids were obtained in this study. Additionally, a plenty of genetic makers were generated by identification of SSRs, which is a powerful tool for molecular breeding and genetics applications in this herb.
Ranking of fibre-reinforced composite plate surface finish quality by wavelet texture analysis

In the automotive and other industries, the visual appearance of external surfaces is a key factor in perceived product quality. Traditionally, the quality of an automotive surface finish has been judged by expert human auditors. A set of 17 fibre-reinforced composite plates was previously manufactured to have a range of surface finish qualities and these plates were ranked by three expert observers and also optically digitally imaged. Following validation of the previous rankings, the wavelet texture analysis (WTA) technique was applied to the digital photographs to derive an instrumental measure of surface finish quality based on the panel images. The rank correlation between the human expert surface finish quality ratings and those from the W TA image analysis process was found to be positive, large and statistically significant. This finding indicates that WTA could form the basis of an inexpensive and practical instrumental method for the ranking of fibre-reinforced composite surface finish quality.
Theoretical and experimental investigation of plasma dynamics for laser- induced machining mechanism Lazer destekli imalatta plazma dinamiklerinin teorik ve deneysel olarak araştırılması

Öz The quality of laser micro-machining process (micro welding, drilling, surface structuring etc.) has vital importance for electronic, aviationaerospace and biomedical industries. Dynamics of laser-induced plasma are determined with some parameters such as laser intensity, beam waist diameter and ambient gas conditions. When the plasma density is limited with a threshold value, the laser energy can not transmitted to the material due to the rarefied plasma. This decoupling threshold has a crucial role for micro-machining operation. In the scope of this paper, the behaviour of the plasma dynamics has been examined both theoretically and experimentally for titanium material and optimal laser intensity threshold was reported to prevent dissipation of beneficial process energy and to compare numerical investigations. Lazer destekli mikro-imalat (mikro kaynak, delme, yüzey yapılandırma vb.) prosesinin kalitesi elektronik, havacılıkuzay ve biyomedikal endüstrileri için özel bir öneme sahiptir. Lazerin oluşturduğu plazmanın dinamikleri lazer güç yoğunluğu, ışın odak çapı ve çevre koşulları tarafından belirlenmektedir. Plazma yoğunluğunda eşik değerine ulaşıldığında plazma korumasına bağlı ışınım kayıpları sebebiyle, lazer enerjisi malzemeye aktarılamamaktadır. Bu ayrışma eşiği mikro-imalat operasyonu için kritik bir role sahiptir. Bu makale kapsamında, titanyum malzeme için plazma dinamikleri teorik ve deneysel olarak incelenmiş ve faydalı prosess enerjisinin kaybını önlemek için optimum lazer yoğunluk eşiği rapor edilmiş ve numerik çalışmalarla karşılaştırılmıştır.
An Investigation into the Effect of Multimedia Training on the Knowledge and Self-Efficacy of Children with Asthma

Introduction: Asthma is one of the most common chronic diseases among children are a global health issue and its rising trend has caused concern about the health system. Therefore, this study was conducted to investigate the effect of multimedia education on the knowledge and self-efficacy of children with asthma in Bushehr. Method: Semi-experimental study. The research community included all children under the age of 12 years with asthma. The samples were selected by available method of 50 people and then divided into two experimental and control groups by simple random method. The data collection tools included questionnaires of demographic data, knowledge and selfefficacy in children with asthma. Validity and reliability of the instruments were measured and then multimedia training was run from the experimental group. Data analysis was performed by SPSS 18 and Mann–Whitney U test. Findings: The mean scores of knowledge and self-efficacy of the research samples in the experimental group increased after multimedia training, so there was a statistically significant difference between the two groups (p <0.001) . Conclusion: Education of children with asthma through multimedia has led to increasing knowledge and self-efficacy. Therefore, considering the effectiveness of multimedia education in children training with asthma, it is recommended that this educational method is used for other chronic childhood diseases.