Miracle-dz/UniFAR-FTUs huggingface dataset:
Hippocampal Sharp Waves and Reactivation during Awake States Depend on Repeated Sequential Experience

Hippocampal firing patterns during behavior are reactivated during rest and subsequent slow-wave sleep. These reactivations occur during transient local field potential (LFP) events, termed sharp waves. Theories of hippocampal processing suggest that sharp waves arise from strengthened plasticity, and that the strengthened plasticity depends on repeated cofiring of pyramidal cells. We tested these predictions by recording neural ensembles and LFPs from rats running tasks requiring different levels of behavioral repetition. The number of sharp waves emitted increased during sessions with more regular behaviors. Reactivation became more similar to behavioral firing patterns across the session. This enhanced reactivation also depended on the regularity of the behavior. Additional studies in CA3 and CA1 found that the number of sharp waves emitted also increased in CA3 recordings as well as CA1, but that the time courses were different between the two structures.
Efficient endosome-to-Golgi transport of Shiga toxin is dependent on dynamin and clathrin

It has previously been shown that Shiga toxin, despite being bound to a glycolipid receptor, can be efficiently endocytosed from clathrin-coated pits. However, clathrin-independent endocytosis is also responsible for a proportion of the toxin uptake in some cells. After endocytosis the toxin can be transported in retrograde fashion to the Golgi apparatus and the endoplasmic reticulum, and then to the cytosol, where it exerts its toxic effect by inactivating ribosomes. In order to investigate the role of dynamin and clathrin in endosome-to-Golgi transport of Shiga toxin, we have used HeLa dynK44A and BHK antisense clathrin heavy chain (CHC) cells that, in an inducible manner, express mutant dynamin or CHC antisense RNA, respectively. In these cell lines, one can study the role of dynamin and clathrin on endosome-to-Golgi transport because they, as shown here, still internalize Shiga toxin when dynamin- and clathrin-dependent endocytosis is blocked. Butyric acid has been shown to sensitize A431 cells to Shiga toxin by increasing the proportion of cell-associated toxin that is transported to the Golgi apparatus and the endoplasmic reticulum. Here, we find that, in HeLa and BHK cells also, butyric acid also increased toxin transport to the Golgi apparatus and sensitized the cells to Shiga toxin. We have therefore studied the role of dynamin and clathrin in both untreated and butyric-acid-treated cells by measuring the sulfation of a modified Shiga B fragment. Our results indicate that endosome-to-Golgi transport of Shiga toxin is dependent on functional dynamin in both untreated cells and in cells treated with butyric acid. Interestingly, the regulation of Shiga toxin transport in untreated and butyric-acid-treated cells differs when it comes to the role of clathrin, because only cells that are sensitized to Shiga toxin with butyric acid need functional clathrin for endosome-to-Golgi transport.
Surgery and perioperative management for post-intubation tracheoesophageal fistula: case series analysis.

BACKGROUND Post-intubation tracheoesophageal fistula (PITEF) is an often mistreated, severe condition. This case series reviewed for both the choice and timing of surgical technique and outcome PITEF patients. METHODS This case series reviewed ten consecutive patients who had undergone esophageal defect repair and airway resection/reconstruction between 2000 and 2014. All cases were examined for patients: general condition, medical history, preparation to surgery, diagnostic work-up, timing of surgery and procedure, fistula size and site, ventilation type, nutrition, post-operative course and complications. RESULTS All patients were treated according to Grillo's technique. Overall, 6/10 patients had undergone a preliminary period of medical preparation. Additionally, 3 patients had already had a tracheostomy, one had had a gastrostomy and 4 had both. One patient had a Dumon stent with enlargement of the fistula. Concomitant tracheal stenosis had been found in 7 patients. The mean length of the fistulas was 20.5 mm (median 17.5 mm; range, 8-45 mm), at a median distance from the glottis of 43 mm (range, 20-68 mm). Tracheal resection was performed in all ten cases. The fistula was included in the resection in 6 patients, while it was excluded in the remaining 4 due to their distance. Post-repair tracheotomy was performed in 3 patients. The procedure was performed in 2 ventilated patients. Morbidity related to fistula and anastomosis was recorded in 3 patients (30%), with one postoperative death (10%); T-Tube placement was necessary in 3 patients, with 2/3 decannulations after long-stenting. Definitive PITEF closure was obtained for all patients. At 5-year follow-up, the 9 surviving patients had no fistula-related morbidity. CONCLUSIONS Primary esophageal closure with tracheal resection/reconstruction seemed to be effective treatment both short and long-term. Systemic conditions, mechanical ventilation, detailed preoperative assessment and appropriate preparation were associated with outcome. Indeed, the 3 patients who had received T-Tube recovered from anastomotic complications.
Why population forecasts should be probabilistic - illustrated by the case of Norway

Abstract Deterministic population forecasts do not give an appropriate indication of forecastuncertainty. Forecasts should be probabilistic, so that their expected accuracy can beassessed. We review three main methods to compute probabilistic forecasts, namelytime series extrapolation, analysis of historical forecast errors, and expert judgement.We illustrate, by the case of Norway up to 2050, how elements of these three methodscan be combined when computing prediction intervals for a population’s future size andage-sex composition. We show the relative importance for prediction intervals ofvarious sources of variance, and compare our results with those of the officialpopulation forecast computed by Statistics Norway. 1 Department of Economics, University of Oslo, P.O. Box 1095 Blindern, N-0317 Oslo, Norway. Phone(+47) 22 85 51 28 or 22 85 51 27 fax (+47) 22 85 50 35. E-mail: n.w. keilman@econ.uio. no.Also Statistics Norway, P.O. Box 8131 Dep, N-0033 Oslo, Norway. 2 Statistics Norway, P.O. Box 8131 Dep, N-0033 Oslo, Norway. Phone (+47) 21 09 46 86.E-mail: dqp@ssb.no
chongmague reveals an essential role for laminin-mediated boundary formation in chordate convergence and extension movements

Although cell intercalation driven by non-canonical Wnt/planar cell polarity (PCP) pathway-dependent mediolateral cell polarity is important for notochord morphogenesis, it is likely that multiple mechanisms shape the notochord as it converges and extends. Here we show that the recessive short-tailed Ciona savignyi mutation chongmague (chm) has a novel defect in the formation of a morphological boundary around the developing notochord. chm notochord cells initiate intercalation normally, but then fail to maintain their polarized cell morphology and migrate inappropriately to become dispersed in the larval tail. This is unlike aimless (aim), a mutation in the PCP pathway component Prickle, which has a severe defect in early mediolateral intercalation but forms a robust notochord boundary. Positional cloning identifies chm as a mutation in the C. savignyi ortholog of the vertebrate alpha 3/4/5 family of laminins. Cs-lamα3/4/5 is highly expressed in the developing notochord, and Cs-lamα3/4/5 protein is specifically localized to the outer border of the notochord. Notochord convergence and extension, reduced but not absent in both chm and aim, are essentially abolished in the aim/aim; chm/chm double mutant, indicating that laminin-mediated boundary formation and PCP-dependent mediolateral intercalation are each able to drive a remarkable degree of tail morphogenesis in the absence of the other. These mechanisms therefore initially act in parallel, but we also find that PCP signaling has an important later role in maintaining the perinotochordal/intranotochordal polarity of Cs-lamα3/4/5 localization.
EFFECTS OF SHORT-TERM TREATMENT WITH IONIC AND CHELATED COPPER ON MEMBRANE REDOX-ACTIVITY INDUCTION IN ROOTS OF IRON - DEFICIENT CUCUMBER PLANTS

The effects of different chemical forms of copper – ionic (CuSO4) and chelated one [Cu(II)HEDTA], applied at micromolar concentrations, on the plasma membrane reductase activity (RA) and proton release in intact roots of cucumber plants grown hydroponically under iron deficiency were studied. Iron starvation provoked high induction of ferric-chelate reductase activity (substrates Fe(III)HEDTA and Fe(III)Citrate) and accelerated cupric-chelate reductase activity (measured with Cu(II)HEDTA and Cu(II)Citrate as electron acceptors), as well as hexacyanoferrate III RA (HCF III). Short-term application of cupric ions in the nutrient solution of irondeficient plants resulted in a dramatic inhibition of Fe(III)HEDTA RA and Cu(II)Citrate RA and stopped H+ release by intact roots. The reductase activity of iron-deficient cucumber roots, measured with HCF III, Fe(III)Citrate or Cu(II)HEDTA, however, was inhibited to a lower extent after cupric ions treatment. In addition, cupric-chelate Cu(II)HEDTA, applied at the same concentration in the nutrient solution of iron-deficient (–Fe) cucumber plants maintained the high stimulation of plasma membrane ferric-chelate RA, enhanced proton release by intact roots and produced additional acceleration of cupric-chelate RA and HCF III reduction in the roots. Application of cupric chelate Cu(II)HEDTA improved the iron-deficiency stress responses of cucumber plants.
Spectral analysis of the Stromlo–APM Survey – II. Galaxy luminosity function and clustering by spectral type

We study the luminosity function and clustering properties of subsamples of local galaxies selected from the Stromlo? APM Survey by the rest-frame equivalent widths of their H and [O ii] emission lines. The bJ luminosity function of star-forming galaxies has a significantly steeper faint-end slope than that for quiescent galaxies: the majority of sub-L* galaxies are currently undergoing significant star formation. Emission-line galaxies are less strongly clustered, both amongst themselves and with the general galaxy population, than are quiescent galaxies. Thus as well as being less luminous, star-forming galaxies also inhabit lower density regions of the Universe than quiescent galaxies.
Integrating Multiple Commodities in a Model of Stochastic Price Dynamics

In this paper we develop a multi-factor model for the joint dynamics of related commodity spot prices in continuous time. We contribute to the existing literature by simultaneously considering various commodity markets in a single, consistent model. In an application we show the economic significance of our approach. We assume that the spot price processes can be characterized by the weighted sum of latent factors. Employing an essentially-affine model structure allows for rich dependencies among the latent factors and thus, the commodity prices. The co-integrated behavior between the different spot price dynamics is explicitly taken into account. Within this framework we derive closed-form solutions of futures prices. The Kalman Filter methodology is applied to estimate the model for crude oil, heating oil and gasoline futures contracts traded on the NYMEX. Empirically, we are able to identify a common non-stationary equilibrium factor driving the long-term price behavior and stationary factors affecting all three markets in a common way. Additionally, we identify factors which only impact subsets of the commodities considered. To demonstrate the economic consequences of our integrated approach, we evaluate the investment into a refinery from a financial management perspective and compare the results with an approach neglecting the co-movement of prices. This negligence leads to radical changes in the project's assessment.
Changes in plasma malondialdehyde concen- tration and some erythrocyte antioxidant enzymes in cows with prolapsus uteri, caesa- rean section, and retained placenta

SUMMARY The periparturient period is especially critical for health and subsequent performance of dairy cows. The aim of the study was to detect the occurrence of an oxidative stress by the determination of the plasma malondialdehyde (MDA) concentration and the erythrocyte glutathione peroxidase (GSH-Px) and catalase (CAT) activities in cows with prolapsus uteri, caesarean section, and retained placenta. A total of 46 Holstein cows (15 cows with normal parturition, 15 cows with retained placenta, 8 cows with prolapsus uteri, 8 cows with caesarean section) of the Research and Practice Farm of Firat University were used in this study. CAT activity did not significantly vary in diseased animals (p > 0.05), while in cows with prolapsus uteri and caesarean section, plasma MDA concentrations significantly increased (p < 0.001), and GSH-Px activity was significantly lowered compared to the control group or to cows with retained placenta (p < 0.01). In retained placenta cows, plasma MDA concentrations and GSH-Px activity were not significantly altered although the enzyme activity tended to increase. These results suggest that the antioxidant systems would be impaired and peroxidation reactions accelerated in cows with prolapsus uteri and caesarean section.
Barrett's Oesophagus in an Achalasia Patient: Immunological Analysis and Comparison with a Group of Achalasia Patients

The aim of the study was to characterize the presence of diverse CD4 and CD8 T cell subsets and regulatory cells in peripheral blood and lower oesophageal sphincter (LES) from a young patient with BE/achalasia without treatment versus achalasia group. In order to characterize the circulating cells in this patient, a cytometric analysis was performed. LES tissue was evaluated by double-immunostaining procedure. Five healthy blood donors, 5 type achalasia patients, and 5 oesophagus tissue samples (gastrooesophageal junction) from transplant donors were included as control groups. A conspicuous systemic inflammation was determined in BE/achalasia patient and achalasia versus healthy volunteer group. Nonetheless, a predominance of Th22, Th2, IFN-α-producing T cells, Tregs, Bregs, and pDCregs was observed in BE/achalasia patient versus achalasia group. A low percentage of Th1 subset in BE/achalasia versus achalasia group was determined. A noticeable increase in tissue of Th22, Th17, Th2, Tregs, Bregs, and pDCregs was observed in BE/achalasia versus achalasia group. Th1 subset was lower in the BE/achalasia patient versus achalasia group. This study suggests that inflammation is a possible factor in the pathogenesis of BE/achalasia. Further research needs to be performed to understand the specific cause of the correlation between BE and achalasia.
Endoscopic Ultrasound and Histology in Chronic Pancreatitis: How Are They Associated?

Objectives This study aimed to correlate endoscopic ultrasound (EUS) criteria and pathology in patients with chronic pancreatitis (CP). Methods Endoscopic ultrasound reports and pathology specimens were reviewed from patients with known or suspected CP who underwent surgery within 1 year of EUS. The following information was abstracted: EUS criteria for CP, corresponding pathology results, and histologic features. The EUS and pathology results were correlated. Results One hundred patients (55 men; mean age, 54 years) underwent a pancreatic resection, median of 50 days (range, 1–363 days). The mean (SD) fibrosis scores in the head and body/tail specimens were 7.9 (3.0) and 6.4 (3.8), respectively (P = 0.02). The main pancreatic duct (MPD) dilation and irregularity were associated with moderate and severe fibrosis. Lobularity with honeycombing was associated with intralobular and interlobular fibrosis. Severe CP was associated with the following: lobularity with honeycombing, hyperechoic foci with shadowing, hyperechoic foci without shadowing, MPD dilation, MPD irregularity, and dilated side branches. Conclusions Endoscopic ultrasound of the pancreas head may be considered in the evaluation of CP. The EUS criteria that were associated with severe CP included the following: lobularity with honeycombing, hyperechoic foci with shadowing, dilated MPD, irregular MPD, and dilated side branches. The importance of pancreatic ductal changes should not be minimized in the evaluation of CP.
Recanalisation of coronary chronic total occlusion by retrograde approach: the first experience in Poland.

BACKGROUND The effectiveness of revascularisation procedures of coronary chronic total occlusion (CTO) has been improved by the introduction of retrograde approach. AIM This study compared the outcomes of CTO revascularisation in a single centre in Krakow, Poland using antegrade and retrograde approach. METHODS From January 2011 to September 2013, 150 patients underwent 159 procedures for percutaneous revascularisation of CTO of 153 vessels. Of the 159 procedures, 124 (78%) were performed using an antegrade approach and 35 (22%) using a retrograde approach. RESULTS All patients were symptomatic, with mean CCS class (2.3 ± 0.6 vs. 2.1 ± 0.7, p = 0.9), mean age (59.2 ± 8.3 vs. 62.6 ± 9.9 years, p = 0.067), and mean number of males (81.3% vs. 81.8%, p = 0.9) similar in the retrograde and antegrade groups, respectively. Most patients in both groups had ejection fraction (EF) ≥ 50% (84.4% vs. 74.4%, respectively). Occlusions assessed according to the J-CTO score showed that 82.9% and 56.4%, respectively, were rated as difficult or very difficult (p < 0.01). Overall procedural success rate was 88.2%, 87.9% in the antegrade, and 74.3% in the retrograde group. Complication rates were low and similar in two groups. However, the retrograde approach was associated with a longer mean fluoroscopy time (47.8 ± 19.6 vs. 19.3 ± 10.0 min, p < 0.00001) and higher volume of contrast fluid (494.6 ± 142.4 vs. 291.9 ± 118.1 mL, p < 0.00001). CONCLUSIONS Using novel equipment with adequate experience allowed high rates of successful revascularisation. The retrograde technique for CTO revascularisation showed good overall success and was safe.
A cost effective 32nm high-K/ metal gate CMOS technology for low power applications with single-metal/gate-first process

For the first time, we have demonstrated a 32 nm high-k/metal gate (HK-MG) low power CMOS platform technology with low standby leakage transistors and functional high-density SRAM with a cell size of 0.157 mum2. Record NMOS/PMOS drive currents of 1000/575 muA/mum, respectively, have been achieved at 1 nA/mum off-current and 1.1 V Vdd with a low cost process. With this high performance transistor, Vdd can be further scaled to 1.0 V for active power reduction. Through aggressive EOT scaling and band-edge work-function metal gate stacks, appropriate Vts and superior short channel control has been achieved for both NMOS and PMOS at Lgate = 30 nm. Compared to SiON-Poly, 30% RO delay reduction has been demonstrated with HK-MG devices. 40% Vt mismatch reduction has been shown with the Tinv scaling. Furthermore, it has been shown that the 1/f noise and transistor reliability exceed the technology requirements.
Bridging indistinct relationships and online loyalty: evidence from online interest-based communities

Purpose – Bridging indistinct relationships and online loyalty has become an important strategy for online interest-based communities' operators and firms. The objective of this study is to draw on the elaboration likelihood model (ELM) to advance our understanding of the influences on the development of relationship quality and the consequent impact on members' loyalty to online communities. Design/methodology/approach – To test the hypotheses the authors identified and selected four interest-based online communities in Taiwan. The online survey yielded 331 completed questionnaires from members of interest-based online communities and the authors analysed the results using the structural equation modelling approach. Findings – The findings show that argument quality and source credibility positively affect members' perceived relationship quality, which has a positive and significant effect on behavioural loyalty, and personal relevance and user expertise positively moderate the relationship between argum.
Differential investment in twin offspring by female pronghorns (Antilocapra americana)

Abstract Differential investment in offspring has been reported for many mammals, often in the context of the Trivers–Willard model of male-biased investment, but evidence of differential investment in pronghorns (Antilocapra americana) is largely lacking. We assessed the causes and consequences of different birth masses of littermate fawns in a pronghorn population in Oregon. The mass differential for co-twins ranged from 0% to 89% (median = 8.35%). Male-biased investment explained the mass differential in opposite-sex litters but not same-sex litters. The mass differential did not result from mothers producing 1 normal-size fawn and 1 runt fawn, and the smaller fawn was not deficient in physiological condition. Only 29% of fawns survived to 8 weeks and both fawns died in 56% of litters, but co-twin mortalities were largely separate events. Mass did not confer a survival advantage when considering all fawns through age 8 weeks, but there was evidence of such an advantage when comparing fawns within litters before age 18 days. Differential investment in fawns might be a bet-hedging strategy in which the mother accepts a lower expected reproductive success in exchange for a lower variance, but neither the mean nor the variance differed between mothers of different-size (>8.35% mass differential) and similar-size (<8.35%) litters. In fact, there was evidence of increased reproductive success for mothers of different-size litters, much of which stemmed from higher survival 4–6 days after birth. Having different-size fawns reduced the chances of sequential mortality, in which a predator killed one fawn then returned to kill the other.
Pubertal Development and Peer Influence on Risky Decision Making

Adolescents engage in more risky behavior when they are with peers and show, on average, heightened susceptibility to peer influence relative to children and adults. However, individual differences in susceptibility to peer influence are not well understood. The current study examined whether the effect of peers on adolescents’ risky decision making was moderated by pubertal status. Participants (58 youth, ages 11-16, 50% male, 63.9% African American) completed a computerized measure of risky decision making, once alone and once in the presence of two peers. Pubertal status was assessed using self-report. Adolescents made riskier decisions in the presence of peers, and more advanced pubertal development predicted greater risky decision making, controlling for chronological age. The effect of peer presence on risky decision making was attenuated for adolescents with more advanced pubertal development. These findings suggest that the presence of peers may override biologically based individual differences in propensity for risk taking.