Miracle-dz/UniFAR-FTUs huggingface dataset:
The Effect of Tin (IV)-Protoporphyrin-IX on Bilirubin Production and Excretion in the Rat

ABSTRACT. : Tin (IV)-protoporphyrin-IXα (tin-heme) may have use in treating neonatal jaundice. To evaluate its effect on bilirubin metabolism, we measured bile-bilirubin excretion in adult, male Sprague-Dawley rats (350-500 g). After a 4-h baseline period, tin-heme (100 umol/kg) or buffer was injected subcutaneously, and bile was collected for 19 h. Bile flow, bile salt excretion, and bile-bilirubin excretion (averaging 600 ± 60 ng/100 g/min for all animals) remained stable in the control period. Tin-heme treatment did not alter bile flow or bile salt excretion, but within 2 h bilirubin output was significantly reduced. The nadir of output was 5 h after injection when it was 380 ± 40 g/100 g/min (p < 0.001). Cumulative excretion over 19 h was reduced 30.8% (p < 0.01). To determine if tin-heme interfered with hepatic uptake or excretion of bilirubin, additional animals were administered intravenous bilirubin at 30 mg/kg/h for 3 h after tin-heme injection. Neither peak bile-bilirubin (37.4 ± 4.68, control; 38.19 ± 3.81/μg/100 g/min, treated) nor cumulative excretion (87.8 ± 4.7, control; 88.9 ± 4.2%, treated) were altered. Biliary excretion of tin-heme was measured under various experimental conditions. When administered alone, maximal excretion was 4 h after injection (4.41 ± 1.58 μg/100 g/min); by 15 h, it fell to 0.024 ± 0.011 μg/100 g/min; 20-h cumulative tin-heme excretion in bile was 21.8 ± 3.1% of the administered dose. Intravenous coadministration of albumin or albumin and bilirubin reduced the peak output but did not alter cumulative excretion of tin-heme. These data indicate that tin-heme reduces endogenous bilirubin formation but does not impair hepatic uptake and excretion. Bile is a major excretory route for tin-heme.
Noradrenergic Modulation of Electrical Coupling in GABAergic Networks of the Hippocampus

Noradrenergic modulation of cortical circuits is involved in information processing, regulation of higher functions, and prevention of epileptic activity. Here, we studied the effects of noradrenaline on the functional connectivity of GABAergic networks of the hippocampus and show that electrical synapses between interneurons are a novel target of noradrenergic modulation in vitro. Application of noradrenaline or of the selective β-adrenergic agonist isoproterenol decreased gap junction-based coupling in paired recordings from stratum lacunosum-moleculare interneurons by ∼40%. Similar results were obtained after pharmacological stimulation of the adenylyl cyclase with forskolin. In contrast, the adenylyl cyclase antagonist MDL12330A [cis-N-(2-phenylcyclopentyl)azacyclotridec-1-en-2-amine] or the specific protein kinase A (PKA) inhibitor H89 (N-[2-(p-bromocinnamyl-amino)ethyl]-5-isoquinolinesulfonamide dihydrochloride) enhanced the basal strength of coupling by ∼30%. In addition, PKA-mediated phosphorylation was critical for both isoproterenol- and forskolin-dependent regulation of coupling, because inclusion of the PKA antagonist KT5720 [(9S,10R,12R)-2,3,9,10,11,12-hexahydro-10-hydroxy-9-methyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3′,2′,1′-kl]pyrrolo[3,4-i][1,6]benzodiazocine-10-carboxylicacid hexyl ester] in the recording pipettes prevented modulation. Lastly, we studied the effects of β-adrenergic modulation on mixed polysynaptic transmission within the GABAergic network. Isoproterenol depressed propagation of GABAA receptor-mediated synaptic currents, but did not change significantly direct GABAergic input, indicating that regulation of electrical coupling adds flexibility to the information flow generated by chemical synapses. In conclusion, activation of β-adrenergic receptors in stratum lacunosum-moleculare GABAergic networks reduces electrical synaptic transmission via a cAMP/PKA signaling cascade, and affects the degree of synaptic divergence within the circuit. We propose that this dynamic modulation and interplay between electrical and chemical synaptic transmission in GABAergic networks contributes to the tuning of memory processes in vivo, and prevents hypersynchronous activity.
Exhaled Nitric Oxide Levels During Treatment of Pediatric Acute Asthma Exacerbations and Association With the Need for Hospitalization

Objectives: To examine how exhaled nitric oxide (eNO) levels measured before and after treatment of asthma exacerbations relate to emergency department (ED) disposition. Methods: We enrolled children 6 to 17 years old treated for asthma exacerbations in a pediatric ED. Using an offline single-breath eNO sampling technique, we collected replicate initial samples before treatment and replicate final samples when disposition was decided. We determined correlations and coefficients of variability of eNO values (parts per billion, ppb) of samples and compared by disposition (hospitalization or discharge) mean initial and final eNO levels and initial-to-final change. Results: Eighty-one subjects had initial and final eNO values; 24 subjects with more severe presentations had final values only. Replicate eNO samples were correlated (initial r = 0.98, final r = 0.99) and had low coefficients of variability (initial, 0.059 ± 0.057; final, 0.061 ± 0.070). For subjects with initial and final values, initial eNO levels were similar by disposition (mean difference, −8.0 ppb; 95% confidence interval [CI], −24.8 to 8.9 ppb), as were final levels (mean difference, −2.8 ppb; 95% CI, −23.8 to 18.2 ppb). Overall, final eNO was higher than initial (36.3 ± 29.7 vs 31.5 ± 23.9 ppb), but only 63% of subjects had any increase. Change in eNO was similar by disposition (mean difference, 4.6 ppb; 95% CI, −3.4 to 12.6). For more severe subjects with final eNO only, eNO was similar by disposition (P = 0.47). Conclusions: For children aged 6 to 17 years with asthma exacerbations, eNO levels can be reliably measured. However, eNO levels measured before treatment or when disposition was determined did not distinguish children needing hospitalization.
Hornerin is a component of the epidermal cornified cell envelopes

A single‐nucleotide polymorphism within the gene encoding hornerin (HRNR) has recently been linked with atopic dermatitis (AD) susceptibility. HRNR shares features with filaggrin, a key protein for keratino‐cyte differentiation, but conflicting reports have been published concerning its expression in the epidermis, and its role is still unknown. To analyze HRNR expression and function in the epidermis, anti‐HRNR antibodies were produced and used in Western blot analysis and immuno‐histochemical, confocal, and immunoelectron microscopy analyses of human skin and of cornified cell envelopes purified from plantar stratum corneum. We also tested whether HRNR was a substrate of transglutaminases. In the epidermis, HRNR was detected at the periphery of keratohyalin granules in the upper granular layer and at the corneocyte periphery in the whole cornified layer. Detected in Western blot analysis as numerous bands, HRNR was relatively insoluble and only extracted from epidermis with urea and/or reducing agents. The presence of HRNR in the purified envelopes was confirmed by immunoelectron microscopy and by Western blot analysis after V8‐protease digestion. HRNR was shown to be a substrate of transglutaminase 3. These data demonstrate that HRNR is a component of cornified cell envelopes of human epidermis. Its reduced expression in AD may contribute to the epidermal barrier defect observed in the disease. —Henry, J., Hsu, C. ‐Y. , Haftek, M. , Nachat, R. , de Koning, H. D. , Gardinal‐Galera, I. , Hitomi, K. , Balica, S. , Jean‐Decoster, C. , Schmitt, A. ‐M. , Paul, C. , Serre, G. , Simon, M. Hornerin is a component of the epidermal cornified cell envelopes. FASEB J. 25, 1567–1576 (2011). www.fasebj. org
UNC-45A is preferentially expressed in epithelial cells and binds to and co-localizes with interphase MTs

UNC-45A is a ubiquitously expressed protein highly conserved throughout evolution. Most of what we currently know about UNC-45A pertains to its role as a regulator of the actomyosin system. However, emerging studies from both our and other laboratories support a role of UNC-45A outside of actomyosin regulation. This includes studies showing that UNC-45A: regulates gene transcription, co-localizes and biochemically co-fractionates with gamma tubulin and regulates centrosomal positioning, is found in the same subcellular fractions where MT-associated proteins are, and is a mitotic spindle-associated protein with MT destabilizing activity in absence of the actomyosin system. Here, we extended our previous findings and show that UNC45A is variably expressed across a spectrum of cell lines with the highest level being found in HeLa cells and in ovarian cancer cells inherently paclitaxel-resistant. Furthermore, we show that UNC-45A is preferentially expressed in epithelial cells, localizes to mitotic spindles in clinical tumor specimens of cancer and co-localizes and co-fractionates with MTs in interphase cells independent of actin or myosin. In sum, we report alteration of UNC45A localization in the setting of chemotherapeutic treatment of cells with paclitaxel, and localization of UNC45A to MTs both in vitro and in vivo. These findings will be important to ongoing and future studies in the field that further identify the important role of UNC45A in cancer and other cellular processes.
Silodosin versus Tamsulosin as Medical Expulsive Therapy for Distal Ureteral Stones: A Prospective Randomized Study

Introduction The aim of this study was to compare the safety and efficacy of silodosin (8 mg) versus tamsulosin (0.4 mg) as a medical expulsive therapy for distal ureteral stones. Methods 136 patients (aged 18 years or older) had presented with renal colic, were diagnosed to have a single, unilateral, radiopaque, proximal ureteral stone (range 4–10 mm in size), and agreed to undergo conservative management. For a 3-week period, 68 patients per group were assigned to the following treatments: Group A (gA) received tamsulosin 0.4 mg once daily, and Group B (gB) received silodosin 8 mg once daily. The time to expulsion, analgesic use, follow-up, endoscopic treatment and adverse effects of drugs were noted. Results Considering all the patients enrolled (136), one patient in gA and two patients in gB were unable to continue the study because of orthostatic hypotension within a week from commencing the treatment. A total of 133 patients (gA: 67; gB: 66) were included in this study. A stone expulsion rate of 61.2% (41 out of 67 patients) was observed in gA, and 80.3% (53 out of 66) was observed in gB. gB showed a statistically relevant advantage in terms of stone expulsion rate (p: 0.003), as well as in terms of expulsion time (weeks) (p: 0.002). No severe complications were recorded in both groups. Four patients (10.2%; 4/39) in gA and ten patients (22.7%; 10/44) in gB experienced retrograde ejaculation (<0.002). Conclusion The patients treated with silodosin showed a significant increase in expulsion rate and a decrease in expulsion time of lower ureteral stones in our study.
Clinical analysis of pulmonary infection in hemodialysis patients

The present study aimed to investigate the pathogen distribution and drug resistance of lung infections in hemodialysis to guide clinical empirical pharmacy. The clinical data of 116 hemodialysis patients with pulmonary infection were analyzed. The majority of the 82 pathogens isolated from the sputa of patients were Gram-negative bacteria (accounting for 71.95%). The results of the drug sensitivity test suggested that Gram-negative bacilli had low resistance rates to piperacillin and tazobactam, imipenem and amikacin, while Gram-positive cocci had a low resistance rate to vancomycin. All resistance rates of the pathogens to other common antimicrobials were >50%. The pathogens resulting in lung infections in hemodialysis patients were mainly Gram-negative bacteria and were significantly resistant to various antibacterials. Results of the this study demonstrate that pathological examination should be performed as early as possible and effective antimicrobial agents should be chosen according to drug sensitivity test results.
Complications in head and neck surgery: a meta-analysis of postlaryngectomy pharyngocutaneous fistula.

OBJECTIVE To summarize the potential risk factors for postlaryngectomy pharyngocutaneous fistula. DATA SOURCES Observational studies in the English-language literature about postlaryngectomy pharyngocutaneous fistula from January 1, 1970, to March 31, 2003. Studies were identified through a MEDLINE search with relevant key words; additional studies were identified through references. STUDY SELECTION We included studies about the site of primary malignancy, type of procedure, and type of closure; studies had to have been based on individual-level data, with a comparison group for each risk factor evaluated. DATA EXTRACTION Data required to calculate the relative risk of fistula associated with commonly reported risk factors were abstracted from the studies, and a meta-analysis using a random-effects approach was performed to estimate a summary relative risk of fistula for each risk factor. The statistical significance of heterogeneity of effects among studies was assessed. DATA SYNTHESIS Of 65 studies identified, 26 met the inclusion criteria. Significant risk factors identified in the pooled analysis included postoperative hemoglobin level less than 12.5 g/dL, prior tracheotomy, preoperative radiotherapy, and preoperative radiotherapy and concurrent neck dissection. The degree of heterogeneity of effects among studies was significant for postoperative hemoglobin level, preoperative radiotherapy, concurrent neck dissection, and comorbid illness. The severity of fistula was greater in patients with a history of radiotherapy. CONCLUSIONS This meta-analysis identified several significant risk factors for postlaryngectomy pharyngocutaneous fistula. The clinical implications of these findings and the potential sources of heterogeneity of effects among studies are discussed.
Mobility modeling and traffic analysis in three-dimensional high-rise building environments

To efficiently plan future personal communications services, we need to solve various mobility/traffic problems in one-dimensional (1-D), two-dimensional (2-D), and three-dimensional (3-D) micro- or pico-cell environments. Although many users exhibit vertical motion inside elevators in high-rise buildings, there have been no studies regarding cell planning which take into account vertical motion with elevators. We extend the previous 3-D indoor mobility modeling by considering the proper boundary conditions on each floor and vertical motions through elevators and modeling mobility in high-rise buildings in order to estimate the number of handoffs. We then propose a blocking probability model with mobility as a traffic model in 3-D indoor environments. Using this model, we can obtain the required number of channels per cell under the given blocking probability constraint. These results can be used in planning the networks of future personal communications services.
Macrophage Migration Inhibitory Factor Homologs of Anisakis simplex Suppress Th2 Response in Allergic Airway Inflammation Model via CD4+CD25+Foxp3+ T Cell Recruitment1

We have cloned the macrophage migration inhibitory factor (MIF)-like protein (Anisakis simplex (As)-MIF) from larvae of the whale worm (Anisakis simplex third-stage larvae). Asthma was induced in the mice using OVA/alum, with or without various concentrations of rAs-MIF treatment before OVA/alum challenge. Treatment with rAs-MIF coupled with OVA/alum during the challenge period induced a complete inhibition of eosinophilia and goblet cell hyperplasia within the lung and profoundly ameliorated the development of lung hyperreactivity. Also, rAs-MIF was shown to reduce profoundly the quantity of Th2-related cytokines (IL-4, IL-5, and IL-13) in the bronchial alveolar lavage fluid and allergen-specific IgG2a in sera. IL-10 and TGF-β levels in the bronchoalveolar lavage fluid of the rAs-MIF-treated group were significantly higher than in the other groups. Additionally, CD4+CD25+Foxp3+ T cells (regulatory T) were recruited to the spleen and lungs of the rAs-MIF-treated mice, but this recruitment was inhibited by anti-rAs-MIF Ab.
Board Composition and Earnings Management an Empirical Evidence Form Pakistani Listed Companies

The study examines the relationship between Board Composition and Earnings Management. A set of listed Companies have been investigated to analyze the relationship for the year 2003 to 2007. Board Composition has been measured by Board independence, and Institutional ownership. Whereas earnings management has been quantified by discretionary accruals. Modified Cross Sectional Jones Model (1995) has been used to determine the Earnings management. Common effect model indicates the presence of negative relationship between institutional ownership and earnings management while we did not get statistically significant relationship between Board independence and earnings management.
Maternal and perinatal outcome following assisted reproductive technology in a tertiary care centre: a retrospective study

Background: Infertility is a major issue and a source of social and psychological suffering for many couples. Introduction of assisted reproductive technology (ART) leads to great relief for couples nowadays.Methods: Present study was a retrospective study conducted at K.E. M hospital, a major tertiary care hospital after approval of the institutional ethics committee. A retrospective analysis of maternal and perinatal outcome of 30 cases of ART conception from the year 2014 to 2018 was done.Results: The mean age of women enrolled into present study was 37.6 years and most common age group was 30-50 years. The main cause of infertility was unexplained (40%), other causes were female factor (33.33%), male factor (16.67%) and combined (10%). 63.33% pregnancies were singleton and 36.67% twin gestations. The most common complication seen was preterm labour (43.33%). The other complications were preeclampsia/eclampsia (33.33%), gestational hypertension, renal failure, gestational diabetes, cardiomyopathy, hepatic failure, HELLP syndrome and DIC. 60% patients had caesarean section and 40% had vaginal delivery.90. 24% neonates had 5-minute Apgar score of more than or equal to 7 and 9.76% had score less than 7. There were 4 stillbirths accounting for 9.76% and out of live births,75.68% babies required NICU admission. There were 5 neonatal deaths (12.2%) and perinatal death rate was 21.95%. Conclusions: ART is associated with a variety of maternal and perinatal complications. Couples should therefore be counseled about these risks while offering them ART as a mode of conception.
Suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, protects dopaminergic neurons from neurotoxin‐induced damage

BACKGROUND AND PURPOSE Prevention or disease‐modifying therapies are critical for the treatment of neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease and Huntington's disease. However, no such intervention is currently available. Growing evidence has demonstrated that administration of histone deacetylase (HDAC) inhibitors ameliorates a wide range of neurologic and psychiatric disorders in experimental models. Suberoylanilide hydroxamic acid (SAHA) was the first HDAC inhibitor approved by the Food and Drug Administration for the sole use of cancer therapy. The purpose of this study was to explore the potential new indications of SAHA for therapy of neurodegenerative diseases in in vitro Parkinson's disease models.
A retrospective analysis of the resorption rate of deproteinized bovine bone as maxillary sinus graft material on cone beam computed tomography.

OBJECTIVES Graft resorption remains a major disadvantage of the various employed materials in maxillary sinus augmentation. Our aim was to assess the percentage of graft volume reduction in deproteinized bovine bone (DBB) using cone beam computed tomography (CBCT). MATERIALS AND METHODS A retrospective analysis of 29 augmented sinuses in 19 patients was conducted. The graft material was either DBB alone or DBB mixed with autologous bone chips (80% DBB/20% bone chips). CBCT scans of the augmented sinuses were obtained pre- and post-operatively up to 2-year follow-up. RESULTS In total, 55 dental implants were placed in the posterior maxillary region following sinus augmentation. The mean height of the alveolar ridge pre-operative was 2.7 ± 1.2 mm and post-operative was 14.13 ± 4.6 mm. The mean percentages of alveolar ridge height reduction were 18.58% (SD14.23%) and 27.62% (SD12.70%), and the mean percentages of graft volume reduction were 19.30% (SD9.19%) and 19.85% (SD9.61%) for DBB and DBB + bone chips, respectively. CONCLUSIONS The results indicate that DBB graft volume resorption is approximately (20 ± 10%) following 2-year follow-up. CBCT is a useful radiographic tool to follow-up changes in sinus graft volumes.
Specific monosynaptic sensory-motor connections form in the absence of patterned neural activity and motoneuronal cell death

The importance of neural activity and motoneuronal cell death in the formation of specific synaptic connections between muscle afferents and motoneurons was studied in chick embryos. Patterned neural activity was blocked by applying d-tubocurarine (dtc) chronically to embryos during the period when sensory-motor connections are formed [stages (St) 28– 42]. Dtc blocks neurogenic muscle contractions, thereby abolishing any temporal correlation between neural activity in motoneurons and stretch- sensitive afferents. The normal pattern of motoneuronal bursting is also blocked (Landmesser and Szente, 1986), as is motoneuronal cell death (Pittman and Oppenheim, 1979). Dtc applications were started more than 1 d before muscle sensory afferent collaterals make anatomical contact with motoneuronal dendrites and continued until St 38–42, when the pattern of synaptic connectivity was examined by recording synaptic potentials intracellularly from identified lumbosacral motoneurons upon stimulation of identified populations of muscle afferents. In both normal and dtc-treated animals, large monosynaptic excitatory potentials were evoked in homonymous motoneurons (those that supply the same muscle as the sensory afferents) and were often observed in motoneurons that supplied synergistic muscles. Monosynaptic potentials were uncommon in motoneurons supplying antagonistic muscles. The overt patterns of sensory-motor connections in normal and dtc-treated embryos were essentially identical. However, the amplitudes of the composite EPSPs recorded in dtc-treated animals were consistently about twice as large as normal. These observations suggest that neither normal patterns of neuronal activity nor motoneuronal cell death play a large role in determining the specificity of connections between the sensory and motor neurons involved in the stretch reflex.