Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparing slow and rapid bolus of ephedrine in pregnant patients undergoing planned cesarean section under spinal anesthesia

Background and Aims: While ephedrine was the preferred drug for treating spinal-induced hypotension in pregnancy, its use has declined because of resultant fetal acidosis. The objective of this randomized control trial was to compare the effects of a slow and rapid bolus of ephedrine on fetal acidosis, maternal blood pressure, and heart rate (HR) during cesarean section performed under spinal anesthesia. Material and Methods: Eighty full-term parturients scheduled for cesarean section under spinal anesthesia were randomly allocated into two groups. While both groups received 6 mg of ephedrine to treat hypotension, Group R (n = 40) received it as a rapid intravenous bolus and Group S (n = 40) received it slowly over 20 s with an infusion pump. The maternal vital parameters were recorded until delivery of the baby using a video camera. Umbilical cord blood was obtained using the three clamp method. Hemodynamic parameters, fetal acidosis, total number of ephedrine bolus used, peak HR after the ephedrine bolus, and occurrence of postoperative nausea and vomiting (PONV) were compared between the groups. Results: Mean increase in HR and blood pressure were significantly higher in Group R than the Group S after the first ephedrine bolus. Umbilical artery pH was significantly lower in Group R than in Group S (7.2 [6.8-7.3] vs. 7.3 [7.3-7.4], P < 0.01). A total number of ephedrine boluses were comparable in the two groups. 35% of the patients had PONV in Group R, whereas none had it in Group S (P < 0.01). Conclusion: Slow bolus of ephedrine is better than rapid bolus to treat spinal-induced hypotension during cesarean section in view of less fetal acidosis.
Spectrophotometric Calibration of pH Electrodes in Seawater Using Purified m-Cresol Purple

This work examines the use of purified meta-cresol purple (mCP) for direct spectrophotometric calibration of glass pH electrodes in seawater. The procedures used in this investigation allow for simple, inexpensive electrode calibrations over salinities of 20–40 and temperatures of 278.15–308.15 K without preparation of synthetic Tris seawater buffers. The optimal pH range is ∼7.0–8.1. Spectrophotometric calibrations enable straightforward, quantitative distinctions between Nernstian and non-Nernstian electrode behavior. For the electrodes examined in this study, both types of behavior were observed. Furthermore, calibrations performed in natural seawater allow direct determination of the influence of salinity on electrode performance. The procedures developed in this study account for salinity-induced variations in liquid junction potentials that, if not taken into account, would create pH inconsistencies of 0.028 over a 10-unit change in salinity. Spectrophotometric calibration can also be used to expeditiously determine the intercept potential (i.e., the potential corresponding to pH 0) of an electrode that has reliably demonstrated Nernstian behavior. Titrations to ascertain Nernstian behavior and salinity effects can be undertaken relatively infrequently (∼weekly to monthly). One-point determinations of intercept potential should be undertaken frequently (∼daily) to monitor for stable electrode behavior and ensure accurate potentiometric pH determinations.
What makes a blood cell based miRNA expression pattern disease specific? - A miRNome analysis of blood cell subsets in lung cancer patients and healthy controls

There is evidence of blood-borne miRNA signatures for various human diseases. To dissect the origin of disease-specific miRNA expression in human blood, we separately analyzed the miRNome of different immune cell subtypes, each in lung cancer patients and healthy individuals. Each immune cell type revealed a specific miRNA expression pattern also dependinging on the cell origin, line of defense, and function. The overall expression pattern of each leukocyte subtype showed great similarities between patients and controls. However, for each cell subtype we identified miRNAs that were deregulated in lung cancer patients including hsa-miR-21, a well-known oncomiR associated with poor lung cancer prognosis that was up-regulated in all leukocyte subtype comparisons of cancer versus controls. While the miRNome of cells of the adaptive immune system allowed only a weak separation between patients and controls, cells of the innate immune system allowed perfect or nearly perfect classification. Leukocytes of lung cancer patients show a cancer-specific miRNA expression profile. Our data also show that cancer specific miRNA expression pattern of whole blood samples are not determined by a single cell type. The data indicate that additional blood components, like erythrocytes, platelets, or exosomes might contribute to the disease specificity of a miRNA signature.
Gastric bypass for treating severe obesity.

Gastric bypass (RY-GBP) has a very small gastric pouch with a 1-cm diameter Roux-Y gastrojejunostomy. RY-GBP is associated with early satiety and an aversion to sweets secondary to dumping syndrome symptoms and has a significantly better weight loss than various gastroplasty procedures, including the vertical banded gastroplasty. However, it may be associated with vitamin B-12 deficiency and iron deficiency anemia in menstruating females, preventable with prophylactic oral iron and vitamin B-12. With an 80% 5 y follow-up, RY-GBP patients lose two-thirds of their excess weight within 2 y, 60% at 5 y, and greater than 50% at 9 y. The RY-GBP can be beaten by nibbling "junk foods" (potato or corn chips). Conversion to a malabsorptive procedure may cause severe malnutrition and fat-soluble vitamin deficiencies and should be used only for "superobese" patients who fail a standard RY-GBP and have severe comorbidity. RY-GBP is the most effective procedure for morbid obesity, especially in patients addicted to "sweets. "
Evaluation of low-dose CT angiography with model-based iterative reconstruction after endovascular aneurysm repair of a thoracic or abdominal aortic aneurysm.

OBJECTIVE The objective of our study was to evaluate image quality and overall adequacy of low-dose CT angiography (CTA) with model-based iterative reconstruction (MBIR) in patients who had undergone endovascular aneurysm repair (EVAR) of a thoracic or abdominal aortic aneurysm. MATERIALS AND METHODS Thirty patients, all of whom had undergone standard-dose CTA performed previously with adaptive statistical iterative reconstruction (ASIR), underwent low-dose CTA for surveillance after EVAR. Two radiologists randomly evaluated both studies, and quality parameters were assessed. The maximal aneurysm diameter was measured, and the images were evaluated to see whether an endoleak was present. The image noise and contrast-to-noise ratio (CNR) were measured. The volume CT dose index and dose-length product were recorded. RESULTS The mean image score for low-dose CTA was acceptable to very good in all categories of assessment. There was no significant difference between low-dose CTA and standard-dose CTA in the evaluation of the stent lumen. Subjective assessments of stent configuration, aneurysm outline, aortic branch vessel outline, overall adequacy of vascular imaging, and overall adequacy of solid organ imaging were superior on standard-dose CTA. Interobserver agreement for endoleak detection was higher for low-dose CTA. There was no significant difference in the mean aneurysm diameter between the two readers on low-dose CTA and standard-dose CTA. The effective radiation dose for low-dose CTA was lower than standard-dose CTA during both the arterial (mean, 4.4 vs 16.2 mSv, respectively) and the delayed (2.4 vs 6.7 mSv) phase acquisitions. The measured image noise was lower (14.7 vs 19.3 HU) and CNR was higher (25.6 vs 17.1) on the low-dose CTA studies than on the standard-dose CTA studies. CONCLUSION Low-dose CTA with MBIR enables up to 73% dose reduction as compared with CTA performed with ASIR while maintaining diagnostic adequacy for CTA surveillance of patients who have undergone EVAR of a thoracic or abdominal aortic aneurysm.
A Study of the Hairiness of Cotton Yarns Part I: Effect of Fiber and Yarn Factors

A combmation of singeing and microscopic techniques has been adopted in the present study to get an insight into the length, nature, and distribution of protruding fibers causing hairmess in yarns. Alter examining various indices, a stutable method of expressing hairmess as the loss in weight on singeing per unit weight of varn has been adopted. Relationships between yarn hairmess and various fiber properties have been investigated in detail, and the factors that influence the preferential protrusion of fiber ends towards the yarn surface are studied by a tracer fiber technique. The results indicate that torsional and flexural rigidity. fiber length, and fineness of cottons are significantly correlated with yarn hairmess. Further, the length of protruding fiber ends and loops is mfluenced not only by the fiber properties of the cottons used but also by processing techniques and factois of varn geometry.
Eye Movements , Strabismus , Amblyopia , and Neuro-Ophthalmology Convergence and Accommodation Development Is Preprogrammed in Premature Infants

METHODS. Thirty-two healthy infants, born at mean 34 weeks gestation (range, 31.2–36 weeks), were compared with 45 healthy full-term infants (mean 40.0 weeks) over a 6-month period, starting at 4 to 6 weeks postnatally. Simultaneous accommodation and convergence to a detailed target were measured using a Plusoptix PowerRefII infrared photorefractor as a target moved between 0.33 and 2 m. Stimulus/response gains and responses at 0.33 and 2 m were compared by both corrected (gestational) age and chronological (postnatal) age.
An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1/Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool

MicroRNAs regulate gene expression in diverse physiological scenarios. Their role in the control of morphogen related signaling pathways has been less studied, particularly in the context of embryonic Central Nervous System (CNS) development. Here, we uncover a role for microRNAs in limiting the spatiotemporal range of morphogen expression and function. Wnt1 is a key morphogen in the embryonic midbrain, and directs proliferation, survival, patterning and neurogenesis. We reveal an autoregulatory negative feedback loop between the transcription factor Lmx1b and a newly characterized microRNA, miR135a2, which modulates the extent of Wnt1/Wnt signaling and the size of the dopamine progenitor domain. Conditional gain of function studies reveal that Lmx1b promotes Wnt1/Wnt signaling, and thereby increases midbrain size and dopamine progenitor allocation. Conditional removal of Lmx1b has the opposite effect, in that expansion of the dopamine progenitor domain is severely compromised. Next, we provide evidence that microRNAs are involved in restricting dopamine progenitor allocation. Conditional loss of Dicer1 in embryonic stem cells (ESCs) results in expanded Lmx1a/b+ progenitors. In contrast, forced elevation of miR135a2 during an early window in vivo phenocopies the Lmx1b conditional knockout. When En1::Cre, but not Shh::Cre or Nes::Cre, is used for recombination, the expansion of Lmx1a/b+ progenitors is selectively reduced. Bioinformatics and luciferase assay data suggests that miR135a2 targets Lmx1b and many genes in the Wnt signaling pathway, including Ccnd1, Gsk3b, and Tcf7l2. Consistent with this, we demonstrate that this mutant displays reductions in the size of the Lmx1b/Wnt1 domain and range of canonical Wnt signaling. We posit that microRNA modulation of the Lmx1b/Wnt axis in the early midbrain/isthmus could determine midbrain size and allocation of dopamine progenitors. Since canonical Wnt activity has recently been recognized as a key ingredient for programming ESCs towards a dopaminergic fate in vitro, these studies could impact the rational design of such protocols.
Feedback and metal enrichment in cosmological smoothed particle hydrodynamics simulations ¿ I. A model for chemical enrichment

We discuss a model for treating chemical enrichment by Type II and Type Ia supernova (SNII and SNIa) explosions in simulations of cosmological structure formation. Our model includes metal-dependent radiative cooling and star formation in dense collapsed gas clumps. Metals are returned into the diffuse interstellar medium by star particles using a local smoothed particle hydrodynamics (SPH) smoothing kernel. A variety of chemical abundance patterns in enriched gas arise in our treatment owing to the different yields and lifetimes of SNII and SNIa progenitor stars. In the case of SNII chemical production, we adopt metal-dependent yields. Because of the sensitive dependence of cooling rates on metallicity, enrichment of galactic haloes with metals can in principle significantly alter subsequent gas infall and the build-up of the stellar components. Indeed, in simulations of isolated galaxies we find that a consistent treatment of metal-dependent cooling produces 25 per cent more stars outside the central region than simulations with a primordial cooling function. In the highly enriched central regions, the evolution of baryons is however not affected by metal cooling, because here the gas is always dense enough to cool. A similar situation is found in cosmological simulations because we include no strong feedback processes which could spread metals over large distances and mix them into unenriched diffuse gas. We demonstrate this explicitly with test simulations which adopt suprasolar cooling functions leading to large changes both in the stellar mass and in the metal distributions. We also find that the impact of metallicity on the star formation histories of galaxies may depend on their particular evolutionary history. Our results hence emphasize the importance of feedback processes for interpreting the cosmic metal enrichment.
College Students' Perceptions of Intimate Partner Cyber Harassment

Little is known about cyber harassment in general, and in order to understand more about online harassment among intimate partners, it is important to examine people's perceptions of this new form of aggression. Using Johnson's typology of relationship violence as a guiding framework, the role of technology in partner violence was explored using data from five focus group interviews. Six themes emerged from the analyses, four of which revealed that this partner violence typology accounted for the aggressive use of technology in dating relationships. The remaining themes centered on the ways in which online harassment differs from offline violence. These findings have important theoretical implications and may inform future prevention and intervention efforts.
To compare and evaluate the efficacy and safety of drotaverine and valethamate bromide

Objectives: To comparatively evaluate the efficacy and safety of drotaverine and valethamate bromide in shortening the duration of labor. Methods: The study was conducted over 200 selected uncomplicated primi and second gravidae with term pregnancy in established labor, at 3 or 4 cm cervical dilatation with adequate uterine contraction. In group I, 100 cases were given injection drotaverine IM, 40 mg every 2 hours up to a maximum of 3 injections, and in group II, the remaining 100 cases received intravenous infusion of valethamate bromide (16 mg in 540 ml of 5% dextrose) at a rate of 16 drops per minute till full dilatation. Duration of first, second and third stage of labor, cervical dilatation rate, injection delivery interval, mode of delivery, side effect if any and neonatal outcome were noted. Results: Injection to full dilatation interval was significantly shorter in group I (113.5±74 min.) in contrast to group II (177.4±105.68 min.) (P<0.05). Irrespective of gravidity, injection to full dilatation duration was shorter in group I being 134.2±80.02 min. vs 189.58±109.24 min. in primi and 82.93±52.09 min. vs 143.56±67.05 min. in second gravidae in group I and group II respectively (P<0.05). Mean cervical dilatation rate was faster in group I as compared to group II in primi as well as in second gravidae (2.89 vs 2.01 cm/hr in primi and 4.55 vs. 2.72 cm/hr in second gravidae). Over all mean cervical dilatation rate was also significantly higher in group I (3.38 cm/hr) as against group II (2.17 cm/hr (P<0.05). Mean injection delivery interval was significantly shorter in group I (135.87±85.94 min vs. 200.19±115.04 min in group II) (P<0.05). There was no significant difference in second and third stage of labor and neonatal outcome. Adverse effects like tachycardia and dryness of mouth was more commonly associated with group II (P<0.001). Conclusion : Intramuscular drotaverine is safe and more effective than valethamate bromide in reducing the duration of first stage and total delivery time.
Mechanisms and benefits of granule cell latency coding in the mouse olfactory bulb

Inhibitory circuits are critical for shaping odor representations in the olfactory bulb. There, individual granule cells can respond to brief stimulation with extremely long (up to 1000 ms), input-specific latencies that are highly reliable. However, the mechanism and function of this long timescale activity remain unknown. We sought to elucidate the mechanism responsible for long-latency activity, and to understand the impact of widely distributed interneuron latencies on olfactory coding. We used a combination of electrophysiological, optical, and pharmacological techniques to show that long-latency inhibition is driven by late onset synaptic excitation to granule cells. This late excitation originates from tufted cells, which have intrinsic properties that favor longer latency spiking than mitral cells. Using computational modeling, we show that widely distributed interneuron latency increases the discriminability of similar stimuli. Thus, long-latency inhibition in the olfactory bulb requires a combination of circuit- and cellular-level mechanisms that function to improve stimulus representations.
Variations in Photosystem I Properties in the Primordial Cyanobacterium Gloeobacter violaceus PCC 7421

We compared the optical properties of the trimeric photosystem (PS) I complexes of the primordial cyanobacterium Gloeobacter violaceus PCC 7421 with those of Synechocystis sp. PCC 6803. Gloeobacter violaceus PS I showed (1) a shorter difference maximum of P700 by approximately 2 nm, (2) a smaller antenna size by approximately 10 chlorophyll (Chl) a molecules and (3) an absence of Red Chls. The energy transfer kinetics in the antennae at physiological temperatures were very similar between the two species due to the thermal equilibrium within the antenna; however, they differed at 77 K where energy transfer to Red Chls was clearly observed in Synechocystis sp. PCC 6803. Taken together with the lower P700 redox potential in G. violaceus by approximately 60 mV, we discuss differences in the optical properties of the PS I complexes with respect to the amino acid sequences of core proteins and further to evolution of cyanobacteria.
Induction by Cationic Antimicrobial Peptides and Involvement in Intrinsic Polymyxin and Antimicrobial Peptide Resistance, Biofilm Formation, and Swarming Motility of PsrA in Pseudomonas aeruginosa

ABSTRACT Pseudomonas aeruginosa is an important opportunistic pathogen that causes infections that can be extremely difficult to treat due to its high intrinsic antibiotic resistance and broad repertoire of virulence factors, both of which are highly regulated. It is demonstrated here that the psrA gene, encoding a transcriptional regulator, was upregulated in response to subinhibitory concentrations of cationic antimicrobial peptides. Compared to the wild type and the complemented mutant, a P. aeruginosa PAO1 psrA::Tn5 mutant displayed intrinsic supersusceptibility to polymyxin B, a last-resort antimicrobial used against multidrug-resistant infections, and the bovine neutrophil antimicrobial peptide indolicidin; this supersusceptibility phenotype correlated with increased outer membrane permeabilization by these agents. The psrA mutant was also defective in simple biofilm formation, rapid attachment, and swarming motility, all of which could be complemented by the cloned psrA gene. The role of PsrA in global gene regulation was studied by comparing the psrA mutant to the wild type by microarray analysis, demonstrating that 178 genes were up- or downregulated ≥2-fold (P ≤ 0.05). Dysregulated genes included those encoding certain known PsrA targets, those encoding the type III secretion apparatus and effectors, adhesion and motility genes, and a variety of metabolic, energy metabolism, and outer membrane permeability genes. This suggests that PsrA might be a key regulator of antimicrobial peptide resistance and virulence.
Using hyperspectral reflectance data for discrimination between healthy and diseased plants, and determination of damage-level in diseased plants

It has been found, through many research works, that hyperspectral reflectance data can be used for studying the pathological conditions of crops. The influence of the pathological status of a crop on its spectral characteristics can be visible or detectable in the visible and/or the near-infrared regions of the electromagnetic spectrum, depending on the spectral effects of the pathological conditions of the crop. Differences in the spectral characteristics between normal (i.e. healthy) crops and others suffering from physiological stress or disease, can be revealed and/or magnified by simply normalising the data properly. Such effects can be achieved by normalising the hyperspectral reflectance data into zero-mean and unit variance vectors (i.e. whitening the data). Spectral-wise and/or band-wise normalisation can be performed here. In the experimental part of this work we used a reference data set consisting of hyperspectral reflectance data vectors and the corresponding field measurements of leaf-damage level in the plants. Then, after normalising the new hyperspectral reflectance data; a nearest neighbour classifier is used to classify our new data against the reference data. The correlation coefficient and the sum of squared differences are used as distance measures (between two vectors) in the nearest neighbour classifier. High correlation is obtained between the classification results and the corresponding field leaf-damage measurements, confirming the usefulness and efficiency of this method for this type of analysis.