Miracle-dz/UniFAR-FTUs huggingface dataset:
Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination

Three biochemically distinct DNA ligase activities have been identified in mammalian cell extracts. We have recently purified DNA ligase II and DNA ligase III to near homogeneity from bovine liver and testis tissue, respectively. Amino acid sequencing studies indicated that these enzymes are encoded by the same gene. In the present study, human and murine cDNA clones encoding DNA ligase III were isolated with probes based on the peptide sequences. The human DNA ligase III cDNA encodes a polypeptide of 862 amino acids, whose sequence is more closely related to those of the DNA ligases encoded by poxviruses than to replicative DNA ligases, such as human DNA ligase I. In vitro transcription and translation of the cDNA produced a catalytically active DNA ligase similar in size and substrate specificity to the purified bovine enzyme. The DNA ligase III gene was localized to human chromosome 17, which eliminated this gene as a candidate for the cancer-prone disease Bloom syndrome that is associated with DNA joining abnormalities. DNA ligase III is ubiquitously expressed at low levels, except in the testes, in which the steady-state levels of DNA ligase III mRNA are at least 10-fold higher than those detected in other tissues and cells. Since DNA ligase I mRNA is also present at high levels in the testes, we examined the expression of the DNA ligase genes during spermatogenesis. DNA ligase I mRNA expression correlated with the contribution of proliferating spermatogonia cells to the testes, in agreement with the previously defined role of this enzyme in DNA replication. In contrast, elevated levels of DNA ligase III mRNA were observed in primary spermatocytes undergoing recombination prior to the first meiotic division. Therefore, we suggest that DNA ligase III seals DNA strand breaks that arise during the process of meiotic recombination in germ cells and as a consequence of DNA damage in somatic cells.
Improving the Robustness Properties of Robot Localization Procedures with Respect to Environment Features Uncertainties

In this paper the localization and environment feature estimation problems are formulated in a stochastic setting and an Extended Kalman Filtering (EKF) approach is proposed for the integration of odometric, gyroscope and sonar measures. As gyroscopic measures are much more reliable than the other ones, the localization algorithm gives rise to a nearly singular EKF. This problem is dealt with defining a lower order non singular EKF.
Unwanted caesarean sections among public and private patients in Brazil: prospective study

Abstract Objective: To assess and compare the preferences of pregnant women in the public and private sector regarding delivery in Brazil. Design: Face to face structured interviews with women who were interviewed early in pregnancy, about one month before the due date, and about one month post partum. Setting: Four cities in Brazil. Participants: 1612 pregnant women: 1093 public patients and 519 private patients. Main outcome measures: Rates of delivery by caesarean section in public and private institutions; women's preferences for delivery; timing of decision to perform caesarean section. Results: 1136 women completed all three interviews; 476 women were lost to follow up (376 public patients and 100 private patients). Despite large differences in the rates of caesarean section in the two sectors (222/717 (31%) among public patients and 302/419 (72%) among private patients) there were no significant differences in preferences between the two groups. In both antenatal interviews, 70-80% in both sectors said they would prefer to deliver vaginally. In a large proportion of cases (237/502) caesarean delivery was decided on before admission: 48/207 (23%) in women in the public sector and 189/295 (64%) in women in the private sector. Conclusions: The large difference in the rates of caesarean sections in women in the public and private sectors is due to more unwanted caesarean sections among private patients rather than to a difference in preferences for delivery. High or rising rates of caesarean sections do not necessarily reflect demand for surgical delivery. What is already known on this topic In Brazil, one quarter of all women deliver in the private sector The rate of caesarean deliveries in the private sector is extremely high (70%) and more than twice that in the public sector, where rates have recently fallen due to a new policy Previous studies in which women were interviewed after birth showed that a substantial proportion of private patients who have caesarean sections would have preferred normal delivery What this study adds In two antenatal interviews, preferences regarding type of delivery were nearly identical among public and private patients and strongly favoured vaginal births Contrary to popular belief, middle and upper class women in Brazil do not want to deliver by caesarean section
Humor in the Eye Tracker: Attention Capture and Distraction from Context Cues

ABSTRACT The humor effect refers to a robust finding in memory research that humorous information is easily recalled, at the expense of recall of nonhumorous information that was encoded in close temporal proximity. Previous research suggests that memory retrieval processes underlie this effect. That is, free recall is biased toward humorous information, which interferes with the retrieval of nonhumorous information. The present research tested an additional explanation that has not been specifically addressed before: Humor receives enhanced attention during information encoding, which decreases attention for context information. Participants observed humorous, nonhumorous positive, and nonhumorous neutral texts paired with novel consumer brands, while their eye movements were recorded using eye-tracker technology. The results confirmed that humor receives prolonged attention relative to both positive and neutral nonhumorous information. This enhanced attention correlated with impaired brand recognition.
Uncivil Youth: Race, Activism, and Affirmative Governmentality

Acknowledgments ix Introduction 1 1. Civilizing Youth against Delinquency 27 2. Youth Organizing and the Nonprofitization of Activism 45 3. Organizing against Youth Criminalization 73 4. Confronting the State 95 Conclusion 121 Notes 131 References 149 Index 165
“Artifactual” arsenate DNA

The recent claim by Wolfe-Simon et al. that the Halomonas bacterial strain GFAJ-1 when grown in arsenate-containing medium with limiting phosphate is able to substitute phosphate with arsenate in biomolecules including nucleic acids and in particular DNA1 arose much skepticism, primarily due to the very limited chemical stability of arsenate esters (see ref. 2 and references therein). A major part of the criticisms was concerned with the insufficient (bio)chemical evidence in the Wolfe-Simon study for the actual chemical incorporation of arsenate in DNA (and/or RNA). Redfield et al. now present evidence that the identification of arsenate DNA was artifactual.
An Exploration of the Impact of Information Privacy Invasion

Even though information privacy literature has been studying the negative influence of privacy concerns on personal information disclosure, the issue is more complex because often the actual behavior of the individual does not reflect the influence. However, limited explanations have been directed toward examining the contradictory phenomenon between privacy concerns and various subsequent behaviors, the so called privacy paradox. Given this apparent gap, a grounded theory approach is undertaken herein, to improve our understanding of the privacy paradox mechanism and other issues with regard to information privacy. The results of this study suggest that prior experiences of individuals with privacy invasion without actual losses decrease the perceived likelihood of actual loss. Further, privacy concerns impact the perceived likelihood of actual loss. Other factors, such as privacy protective efficacy, privacy apathy, etc, that are related to the inherent role of privacy concerns in behavior are also discussed in the study.
Generalized Hyperbolic Discounting, Borrowing Aversion, and Debt Holding

Analysis of an original, broad, internet-based survey reveals that debt holding is related to three aspects of time discounting: (i) present bias, measured by the degree of declining impatience in the generalized hyperbolic discount function; (ii) borrowing aversion, captured by a sign effect - discounting future losses at a lower rate than future gains; and (iii) impatience, measured by the overall discount rate. Present-biased respondents are classified as naĩve if their answers reveal them to be time-inconsistent procrastinators, and classified as sophisticated otherwise. Naĩve respondents with more steeply declining impatience are more likely to be debtors, and are likely to have larger amounts of debt, whereas sophisticates display only insignificant positive association between declining impatience and debt holding. Responses indicative of a sign effect are negatively associated with debt holding. The marginal effect on debt of such a sign effect is larger in magnitude than the effect of one standard deviation increases both in declining impatience and in impatience. Survey responses indicative of high or declining impatience are associated with high debt-to-income ratios, borrowing on credit cards, and the experiences of having borrowed unsecured consumer loans, of having engaged in debt-restructuring, or of having declared personal bankruptcy.
Aesthetics-Guided Summarization from Multiple User Generated Videos

In recent years, with the rapid development of camera technology and portable devices, we have witnessed a flourish of user generated videos, which are gradually reshaping the traditional professional video oriented media market. The volume of user generated videos in repositories is increasing at a rapid rate. In today's video retrieval systems, a simple query will return many videos which seriously increase the viewing burden. To manage these video retrievals and provide viewers with an efficient way to browse, we introduce a system to automatically generate a summarization from multiple user generated videos and present their salience to viewers in an enjoyable manner. Among multiple consumer videos, we find their qualities to be highly diverse due to various factors such as a photographer's experience or environmental conditions at the time of capture. Such quality inspires us to include a video quality evaluation component into the video summarization since videos with poor qualities can seriously degrade the viewing experience. We first propose a probabilistic model to evaluate the aesthetic quality of each user generated video. This model compares the rich aesthetics information from several well-known photo databases with generic unlabeled consumer videos, under a human perception component indicating the correlation between a video and its constituting frames. Subjective studies were carried out with the results indicating that our method is reliable. Then a novel graph-based formulation is proposed for the multi-video summarization task. Desirable summarization criteria is incorporated as the graph attributes and the problem is solved through a dynamic programming framework. Comparisons with several state-of-the-art methods demonstrate that our algorithm performs better than other methods in generating a skimming video in preserving the essential scenes from the original multiple input videos, with smooth transitions among consecutive segments and appealing aesthetics overall.
Privatization or Public Enterprise Reform?: International Case Studies with Implications for Public Management

Preface by Ali Farazmand Privatization Or Public Enterprise Reform? : Implications for Public Management, An Introduction by Ali Farazmand Government Contracting Services and Public Enterprise Reform Contracting Between Governments and the Private Sector: Private Haven or Public Risk? By Guy Callender and Judy Johnston Public Enterprise Reform and Performance Contracting by Anthony Bennett Privatization and Public Enterprise Reform in More Developed Nations France: Privatization in France by Vivien Schmidt Germany: The German Privatization Debate by Margaret Reid U.S.A. : The Contracting Conundrum: Or, Do We Really Understand Privatization by Nicholas Henry United Kingdom: Privatization and the New Public Enterprise: The New Labour Agenda for Public Management by Francis Terry Australia: Privatization and Public Enterprise in Australia by F. Collyer, J. McMaster and R. Wettenhall Privatization and Public Enterprise Reform in Developing Countries Iran: Public Enterprise and Reform in Iran by Ali Farazmand India: Privatization and Public Enterprise Reform in India by S. Maheshwari Privatization in Developing Countries: Formal Causes, Critical Reasons, and Adverse Impacts by Shamsul Haque Challenges to Privatization in Developing Countries: A New Look by W. Kurtz and R. Cunningham Index
Insulin Resistance in Non-Obese Subjects Is Associated with Activation of the JNK Pathway and Impaired Insulin Signaling in Skeletal Muscle

Background The pathogenesis of insulin resistance in the absence of obesity is unknown. In obesity, multiple stress kinases have been identified that impair the insulin signaling pathway via serine phosphorylation of key second messenger proteins. These stress kinases are activated through various mechanisms related to lipid oversupply locally in insulin target tissues and in various adipose depots. Methodology/Principal Findings To explore whether specific stress kinases that have been implicated in the insulin resistance of obesity are potentially contributing to insulin resistance in non-obese individuals, twenty healthy, non-obese, normoglycemic subjects identified as insulin sensitive or resistant were studied. Vastus lateralis muscle biopsies obtained during euglycemic, hyperinsulinemic clamp were evaluated for insulin signaling and for activation of stress kinase pathways. Total and regional adipose stores and intramyocellular lipids (IMCL) were assessed by DXA, MRI and 1H-MRS. In muscle of resistant subjects, phosphorylation of JNK was increased (1.36±0.23 vs. 0.78±0.10 OD units, P<0.05), while there was no evidence for activation of p38 MAPK or IKKβ. IRS-1 serine phosphorylation was increased (1.30±0.09 vs. 0.22±0.03 OD units, P<0.005) while insulin-stimulated tyrosine phosphorylation decreased (10.97±0.95 vs. 0.89±0.50 OD units, P<0.005). IMCL levels were twice as high in insulin resistant subjects (3.26±0.48 vs. 1.58±0.35% H2O peak, P<0.05), who also displayed increased total fat and abdominal fat when compared to insulin sensitive controls. Conclusions This is the first report demonstrating that insulin resistance in non-obese, normoglycemic subjects is associated with activation of the JNK pathway related to increased IMCL and higher total body and abdominal adipose stores. While JNK activation is consistent with a primary impact of muscle lipid accumulation on metabolic stress, further work is necessary to determine the relative contributions of the various mediators of impaired insulin signaling in this population.
Optimal Base Wavelet Selection for ECG Noise Reduction Using a Comprehensive Entropy Criterion

The selection of an appropriate wavelet is an essential issue that should be addressed in the wavelet-based filtering of electrocardiogram (ECG) signals. Since entropy can measure the features of uncertainty associated with the ECG signal, a novel comprehensive entropy criterion Ecom based on multiple criteria related to entropy and energy is proposed in this paper to search for an optimal base wavelet for a specific ECG signal. Taking account of the decomposition capability of wavelets and the similarity in information between the decomposed coefficients and the analyzed signal, the proposed Ecom criterion integrates eight criteria, i.e., energy, entropy, energy-to-entropy ratio, joint entropy, conditional entropy, mutual information, relative entropy, as well as comparison information entropy for optimal wavelet selection. The experimental validation is conducted on the basis of ECG signals of sixteen subjects selected from the MIT-BIH Arrhythmia Database. The Ecom is compared with each of these eight criteria through four filtering performance indexes, i.e., output signal to noise ratio (SNRo), root mean square error (RMSE), percent root mean-square difference (PRD) and correlation coefficients. The filtering results of ninety-six ECG signals contaminated by noise have verified that Ecom has outperformed the other eight criteria in the selection of best base wavelets for ECG signal filtering. The wavelet identified by the Ecom has achieved the best filtering performance than the other comparative criteria. A hypothesis test also validates that SNRo, RMSE, PRD and correlation coefficients of Ecom are significantly different from those of the shape-matched approach (α = 0.05 , two-sided t- test).
A Wideband Transformer-Coupled CMOS Power Amplifier for $X$-Band Multifunction Chips

This paper presents a wideband transformer-coupled CMOS power amplifier (PA). On-chip transmission-line transformers are used as key components of matching networks at output, input, and interstage. The wideband on-chip transformer is harnessed without any additional inductive devices so that a wideband power characteristic can be achieved. The PA is fabricated using a 0.18-μm CMOS process. It provides a saturated output power of 21.5 dBm with the power-added efficiency (PAE) of 20.3%, and the output 1-dB gain-compressed power (P1 dB) is 20.2 dBm with the PAE of 14.8% at 9.5 GHz, respectively. The small-signal gain is 25.3 dB and the 3-dB bandwidth is 6.5 GHz (6.5-13 GHz). The die area is 1.34 mm × 0.47 mm. Among the reported X/Ku-band CMOS PAs, this amplifier achieves the highest figure of merit, and also shows suitable performances for phased-array systems.
Cross-Sectional Regularities in the Response of Stock Prices to Bond Rating Changes

This paper examines whether a firm's stock price response to the new information provided by a bond rating change is related to its net intangible assets. A variable used to measure net intangible assets, which is based on current cost data, is found to have significant explanatory power in cross-sectional regressions for the set of rating downgrades.
Surveillance and Treatment of Non-Muscle-Invasive Bladder Cancer in the USA

Seventy percent of newly diagnosed bladder cancers are classified as non-muscle-invasive bladder cancer (NMIBC) and are often associated with high rates of recurrence that require lifelong surveillance. Currently available treatment options for NMIBC are associated with toxicities that limit their use, and actual practice patterns vary depending upon physician and patient characteristics. In addition, bladder cancer has a high economic and humanistic burden in the United States (US) population and has been cited as one of the most costly cancers to treat. An unmet need exists for new treatment options associated with fewer complications, better patient compliance, and decreased healthcare costs. Increased prevention of recurrence through greater adherence to evidence-based guidelines and the development of novel therapies could therefore result in substantial savings to the healthcare system.
Misvaluation and Insider Trading Incentives for Accrual‐Based and Real Earnings Management

We investigate the incentives that misvaluation creates for: (1) insider trading; and (2) concurrent earnings management through both accruals and real activities. Managers of overvalued firms have an incentive to sustain overvaluation through income increasing earnings management and, at the same time, to sell their shares (Jensen, ). Managers of undervalued firms benefit from buying their firm's shares, however the negative effects of downward earnings management may offset incentives to enhance trading advantages. The results indicate that managers of both over- and under-valued firms act opportunistically, managing earnings upward (downward) with accruals while selling (buying) shares. The Sarbanes-Oxley Act of 2002 (SOX) has been largely ineffective in eliminating trading motivated earnings management. Finally, we do not find evidence of a relationship between managerial trading and real earnings management.
Integrated Analysis of miRNA and mRNA Expression in Childhood Medulloblastoma Compared with Neural Stem Cells

Medulloblastoma (MB) is the most common malignant brain tumor in children and a leading cause of cancer-related mortality and morbidity. Several molecular sub-types of MB have been identified, suggesting they may arise from distinct cells of origin. Data from animal models indicate that some MB sub-types arise from multipotent cerebellar neural stem cells (NSCs). Hence, microRNA (miRNA) expression profiles of primary MB samples were compared to CD133+ NSCs, aiming to identify deregulated miRNAs involved in MB pathogenesis. Expression profiling of 662 miRNAs in primary MB specimens, MB cell lines, and human CD133+ NSCs and CD133− neural progenitor cells was performed by qRT-PCR. Clustering analysis identified two distinct sub-types of MB primary specimens, reminiscent of sub-types obtained from their mRNA profiles. 21 significantly up-regulated and 12 significantly down-regulated miRNAs were identified in MB primary specimens relative to CD133+ NSCs (p<0.01). The majority of up-regulated miRNAs mapped to chromosomal regions 14q32 and 17q. Integration of the predicted targets of deregulated miRNAs with mRNA expression data from the same specimens revealed enrichment of pathways regulating neuronal migration, nervous system development and cell proliferation. Transient over-expression of a down-regulated miRNA, miR-935, resulted in significant down-regulation of three of the seven predicted miR-935 target genes at the mRNA level in a MB cell line, confirming the validity of this approach. This study represents the first integrated analysis of MB miRNA and mRNA expression profiles and is the first to compare MB miRNA expression profiles to those of CD133+ NSCs. We identified several differentially expressed miRNAs that potentially target networks of genes and signaling pathways that may be involved in the transformation of normal NSCs to brain tumor stem cells. Based on this integrative approach, our data provide an important platform for future investigations aimed at characterizing the role of specific miRNAs in MB pathogenesis.
ECONOMIC IMPLICATIONS OF CONGESTION

This report will be of interest to individuals who are concerned about the economic implications of congestion. This study examines how traffic congestion affects producers of economic goods and services in terms of business costs, productivity, and output, and how producers are variously sensitive to congestion. Past attempts to assess the economic implications of congestion have found that this is a difficult relationship to document. This study should be viewed as an incremental step toward a broader definition of the economic costs of congestion. The research shows the many facets of congestion impacts on businesses and local economies, by illustrating the types of data necessary to document those costs and demonstrating how analysis can be carried out and ultimately improved.