Miracle-dz/UniFAR-FTUs huggingface dataset:
Monitoring of minimal residual disease in NPM1-mutated acute myeloid leukemia: a study from the German-Austrian acute myeloid leukemia study group.

PURPOSE To evaluate the prognostic value of minimal residual disease (MRD) in patients with acute myeloid leukemia (AML) with NPM1 mutation (NPM1(mut)). PATIENTS AND METHOD RNA-based real-time quantitative polymerase chain reaction (RQ-PCR) specific for the detection of six different NPM1(mut) types was applied to 1,682 samples (bone marrow, n = 1,272; blood, n = 410) serially obtained from 245 intensively treated younger adult patients who were 16 to 60 years old. RESULTS NPM1(mut) transcript levels as a continuous variable were significantly associated with prognosis after each treatment cycle. Achievement of RQ-PCR negativity after double induction therapy identified patients with a low cumulative incidence of relapse (CIR; 6.5% after 4 years) compared with RQ-PCR-positive patients (53.0%; P < .001); this translated into significant differences in overall survival (90% v 51%, respectively; P = .001). After completion of therapy, CIR was 15.7% in RQ-PCR-negative patients compared with 66.5% in RQ-PCR-positive patients (P < .001). Multivariable analyses after double induction and after completion of consolidation therapy revealed higher NPM1(mut) transcript levels as a significant factor for a higher risk of relapse and death. Serial post-treatment assessment of MRD allowed early detection of relapse in patients exceeding more than 200 NPM1(mut)/10(4) ABL copies. CONCLUSION We defined clinically relevant time points for NPM1(mut) MRD assessment that allow for the identification of patients with AML who are at high risk of relapse. Monitoring of NPM1(mut) transcript levels should be incorporated in future clinical trials to guide therapeutic decisions.
Mechanism of Rain Erosion. Part 2. A Critical Review of Erosion by Water Drop Impact

Abstract : A literature review is presented for erosion of surfaces by water-drop impact. The types of experimental apparatus employed are discussed and include the vibratory method, and the wheel-and-jet, rotating-arm, interrupted-jet, and shock-wave apparatus. Factors discussed which determine the extent of erosion are temperature, time, drop velocity and shape, specimen velocity and shape, drop size and material, specimen size and material, blemishes, and protective finishes and coatings. The results of microscopic and x-ray studies are discussed. Liquid-solid-impact discussions include the steps in drop collapse, pressure under an impacting water drop, and the results of shock-wave studies. A review of the erosion process includes studies of fatigue ranges, the chemical and mechanical nature of erosion, initiation centers, and erosion mechanisms.
A novel hypothermic machine perfusion system using a LifePort Kidney Transporter for the preservation of rat liver

The protective mechanisms for liver preservation associated with hypothermic machine perfusion (HMP) remain unclear. However, the lack of a common and portable HMP system for rat livers limits the study of HMP. The present study aimed to develop a novel, modified HMP system using a LifePort Kidney Transporter for preserving rat livers. A simple ‘Y’ shunt combined with a pressoreceptor for flow and pressure regulation was adapted to perfuse rat livers via the portal vein continuously using a LifePort Kidney Transporter under its ‘prime mode’ setting. An electronic scale was installed under the liver container to calculate the portal inflow according to the association with weight, density and volume of the perfusate. A total of 10 rat livers underwent 6 h of HMP using histidine-tryptophan-ketoglutarate solution enriched with acridine orange (AO) and propidium iodide (PI). The perfusion status of HMP was assessed by comparison of AO+PI-positive cell count in core region (CR) and peripheral region (PR) of rat liver under fluorescence microscopy. The dynamics (inflow, pressure and intrahepatic resistance of perfusion) were assessed to identify whether this system met the demands for HMP of rat livers. Biochemical [alanine transaminase (ALT), lactate dehydrogenase (LDH) and endothelin levels] and histological parameters (sinusoidal dilatation, endothelial cell detachment and vacuolization) were measured to determine cellular damage associated with HMP. No significant difference was observed between the CR and PR according to the comparison of the AO+PI-positive cell count, which indicated that complete perfusion was achieved. Intrahepatic resistance significantly decreased during the initial 3 h of HMP (P<0.01), but remained stable during the final 3 h. ALT and LDH levels significantly increased over the 6 h HMP duration: ALT (0 h, 42.67±5.81 U/l; 3 h, 90.67±6.74 U/l; 6 h, 164.33±7.31 U/l; P<0.01) and LDH (0 h, 492.90±90.20 U/l; 3 h, 973.53±97.4; 6 h, 1,843.40±85.78 U/l; P<0.01) However, the levels of endothelin and oxygen consumption were constant throughout HMP. Furthermore, histological analysis indicated sinusoidal dilation was significantly increased in the post-HMP group compared with the pre-HMP group (P<0.01); however, no other significant differences were observed. Combined with the results of ATP test (640.64±29.46 nmol/l) and bile production (4.88±0.69 µl/h/g of liver) at the end of HMP, the present results demonstrated minimal cellular injury associated with HMP while retaining the dependability and portability of the LifePort Kidney Transporter, which suggests the modified HMP system met the demands required and may be suitable for rat liver preservation.
Risk factors and effective management of preeclampsia

Preeclampsia, a hypertensive disorder of pregnancy is estimated to complicate 2%–8% of pregnancies and remains a principal cause of maternal and fetal morbidity and mortality. Preeclampsia may present at any gestation but is more commonly encountered in the third trimester. Multiple risk factors have been documented, including: family history, nulliparity, egg donation, diabetes, and obesity. Significant progress has been made in developing tests to predict risk of preeclampsia in pregnancy, but these remain confined to clinical trial settings and center around measuring angiogenic profiles, including placental growth factor or newer tests involving metabolomics. Less progress has been made in developing new treatments and therapeutic targets, and aspirin remains one of the few agents shown to consistently reduce the risk of developing preeclampsia. This review serves to discuss recent advances in risk factor identification, prediction techniques, and management of preeclampsia in antenatal, intrapartum, and postnatal patients.
Coincident Generation of Pyramidal Neurons and Protoplasmic Astrocytes in Neocortical Columns

Astrocytes, one of the most common cell types in the brain, are essential for processes ranging from neural development through potassium homeostasis to synaptic plasticity. Surprisingly, the developmental origins of astrocytes in the neocortex are still controversial. To investigate the patterns of astrocyte development in the neocortex we examined cortical development in a transgenic mouse in which a random, sparse subset of neural progenitors undergoes CRE/lox recombination, permanently labeling their progeny. We demonstrate that neural progenitors in neocortex generate discrete columnar structures that contain both projection neurons and protoplasmic astrocytes. Ninety-five percent of developmental cortical columns labeled in our system contained both astrocytes and neurons. The astrocyte to neuron ratio of labeled cells in a developmental column was 1:7.4, similar to the overall ratio of 1:8.4 across the entire gray matter of the neocortex, indicating that column-associated astrocytes account for the majority of protoplasmic astrocytes in neocortex. Most of the labeled columns contained multiple clusters of several astrocytes. Dividing cells were found at the base of neuronal columns at the beginning of gliogenesis, and later within the cortical layers, suggesting a mechanism by which astrocytes could be distributed within a column. These data indicate that radial glia are the source of both neurons and astrocytes in the neocortex, and that these two cell types are generated in a spatially restricted manner during cortical development.
Study on Extraction of Polyphenol from Grape Peel Microwave-Assisted Activity

In this study, we performed a single-factor experiment and an orthogonal experiment to evaluate different processing conditions during the extraction of polyphenols from grape skin using microwave-assisted ethanol extraction. In the study, we found that the best conditions for polyphenol extraction using microwave assistance were as follows: 50% ethanol (40 mL) was used to extract polyphenols from grape skin (1 g) while in a 540-W microwave for 3 min.
Performance and physiological effects of different descending strategies for cross-country mountain biking

Abstract This study investigated the performance-related feasibility and physiological benefits of purposefully eliminating propulsive work while descending in mountain biking and compared values to those measured during road descending. Participants cycled uphill on a road at race pace before descending over three conditions (off-road pedalling; off-road coasting; road coasting). Relatively low power output during off-road pedalling was associated with a greater oxygen uptake (p < .01) when compared with off-road coasting despite no difference in vibration exposure (p > .05). Importantly, pedalling did not invoke a performance benefit (p > .05) on the descent used in this study. Significantly greater heart rate and oxygen uptake (both p < .01) were observed between road and off-road descending, likely caused by the increase in terrain-induced vibrations (p < .01) experienced between the bicycle and rider. Results indicate that reducing propulsive work during descending can improve recovery without being disadvantageous to performance. Similarly, the vibrations experienced during road descending are relatively low, and further reduce oxygen cost. In an effort to increase efficiency, it is recommended that mountain bike athletes focus on skills to increase descending speed without the addition of pedalling, and that equipment be used to decrease vibrations nearer to those seen on the road.
Apigenin induces cell shrinkage in Candida albicans by membrane perturbation

Apigenin, a natural flavone, has been well characterized for its their anticarcinogenic property; however, its bioactivity against pathogenic fungi has not been investigated in detail. In this study, we examined the antifungal activity and mode of action of apigenin. Apigenin inhibited the growth of fungal pathogens, which induced superficial infection and reduced biofilm mass. Three-dimensional flow cytometric analysis demonstrated that apigenin induced morphological changes, especially cell shrinkage, in Candida albicans. We investigated the cause of cell shrinkage using the cyanine dye 3,3΄-dipropylthiacarbocyanine iodide. Results revealed that apigenin altered the cell membrane potential. Apigenin also induced membrane dysfunction, and increased cell permeability to 1,6-diphenyl-1,3,5-hexatriene and propidium iodide. We observed the influx and efflux of fluorescent molecules of varying molecular weights and radii across large unilamellar vesicles and live cells that had been treated with apigenin. Membrane disruption facilitates the release of small intracellular constituents such as ions and sugars, but not proteins. These findings suggested that apigenin exerted an antifungal activity by inducing membrane disturbances, which led to cell shrinkage and leakage of intracellular components.
Climate change impacts on net primary production ( NPP ) and export production ( EP ) regulated by increasing stratification and phytoplankton community structure in the CMIP 5 models

We examine climate change impacts on net primary production (NPP) and export production (sinking particulate flux; EP) with simulations from nine Earth system models (ESMs) performed in the framework of the fifth phase of the Coupled Model Intercomparison Project (CMIP5). Global NPP and EP are reduced by the end of the century for the intense warming scenario of Representative Concentration Pathway (RCP) 8.5. Relative to the 1990s, NPP in the 2090s is reduced by 2–16 % and EP by 7–18 %. The models with the largest increases in stratification (and largest relative declines in NPP and EP) also show the largest positive biases in stratification for the contemporary period, suggesting overestimation of climate change impacts on NPP and EP. All of the CMIP5 models show an increase in stratification in response to surface–ocean warming and freshening, which is accompanied by decreases in surface nutrients, NPP and EP. There is considerable variability across the models in the magnitudes of NPP, EP, surface nutrient concentrations and their perturbations by climate change. The negative response of NPP and EP to increasing stratification reflects primarily a bottom-up control, as upward nutrient flux declines at the global scale. Models with dynamic phytoplankton community structure show larger declines in EP than in NPP. This pattern is driven by phytoplankton community composition shifts, with reductions in productivity by large phytoplankton as smaller phytoplankton (which export less efficiently) are favored under the increasing nutrient stress. Thus, the projections of the NPP response to climate change are critically dependent on the simulated phytoplankton community structure, the efficiency of the biological pump and the resulting levels of regenerated production, which vary widely across the models. Community structure is represented simply in the CMIP5 models, and should be expanded to better capture the spatial patterns and climate-driven changes in export efficiency.
The usage of empty fruit bunch (EFB) and palm pressed fibre (PPF) as substrates for the cultivation of pleurotus ostreatus

At present Malaysia is the largest exporter of palm oil in the international market. In the process of extraction of palm oil from oil palm fruit, biomass materials such as palm empty fruit bunch (EFB) and palm pressed fibre (PPF) are generated as waste products. This research was undertaken to evaluate the use of empty fruit bunch (EFB) and shredded palm pressed fibres as the substrates for the cultivation of Pleurotus ostreatus (oyster mushroom) which currently use sawdust of rubber trees. Five different substrates were prepared either alone or in combinations with rubber tree sawdust. These substrates were supplemented with fixed ratio of rice bran and limestone to increase the yield of Pleurotus ostreatus. The highest yield observed is from substrate comprising 50% PPF and 50% rubber tree sawdust with a mean yield of 0.19375 kg. Both shredded palm pressed fibre and empty fruit bunch show potential as substrate for the cultivation of Pleurotus ostreatus.
Preferences Versus Strategies as Explanations for Culture-Specific Behavior

In this article, we present a new framework for interpreting cultural differences in behavior—what we call the institutional approach. In this framework, individuals' behaviors are conceptualized as strategies adapted to various incentive structures. Cultural differences in behavior are thus viewed as differences in the default adaptive strategies that individuals come to rely on in unclear situations. Through two studies, we demonstrate that the East Asian “preference” for conformity is actually a default strategy to avoid accrual of negative reputation. When the possibility for negative evaluations in a given situation was clearly defined, cultural differences in the tendency for uniqueness disappeared. This approach carries important implications to psychologists who interpret cultural differences in behavior in terms of preferences, and can serve as a common framework branching out toward other disciplines in the social sciences.
Tensile mechanical properties in PP/SEBS/Microballon composites under impact loadings

Purpose: The mechanical properties of the syntactic polymer foams at the intermediate and high strain rates were not understood comprehensively. Then, this study characterizes the tensile mechanical properties of the polymer syntactic composites at high strain rates. Design/methodology/approach: Eight kinds of syntactic foams and one neat PP/SEBS specimens are prepared at the same manufacturing process: 0, 2, 4, 8, 10, 20, 30, 40 and 50 volume percents of microballoons in the PP/SEBS blend matrix. Tensile tests are conducted at strain rates ranged from 0.3 to 100 s-1. Apparent elastic modulus, yield stress and rupture strain are measured and the effects of microballoons on the mechanical properties are studied. In addition, the experimental results are compared with analytical model for closed-cell foam and the effects of the density of the PP/SEBS/microballoon composite on both apparent elastic modulus and yield stress are discussed. Findings: The apparent elastic moduli of PP/SEBS/microballoon syntactic composites follow the Gibson-Ashby law at the nominal strain rate of 100 s-1. The yield stress of PP/SEBS/microballoon syntactic composites follow the simple rule of mixture at the relative densities larger than 0.9. The material ductility decreases drastically once the microballoons are blended in the matrix material. Research limitations/implications: The influence of the local strain rate caused by the heterogeneous microstructure on the mechanical properties is to be further explored. Practical implications: In the automobile applications, the thermoplastic polymer syntactic foams are believed to have many advantages because the usual commercial extruders or injection moulding machines are applicable for producing them, leading to the more light-weight polymeric components. Originality/value: The present study investigates the effects of the strain rate and density on the tensile mechanical properties comprehensively in the polymer syntactic foams.
Transition to adult services for young people with mental health needs: A systematic review

Background: Young people’s transition from child and adolescent (CAMHS) to adult mental health services (AMHS). Objectives: To systematically review evidence on the effectiveness of different models of CAMHS–AMHS transitional care, service user and staff perspectives, and facilitators of/barriers to effective CAMHS–AMHS transition. Data sources: A systematic search in May 2012 of Medline, PsycINFO, CINAHL, EMBASE, AMED, Health Business Elite, HMIC, Cochrane Database, Web of Science and ASSIA; ancestral searches; and consultation with experts in the field. Study selection: Qualitative, quantitative and mixed-methods primary research on the CAMHS–AMHS health-care transition of young people (aged 16–21 years) with mental health problems. Data extraction: Two reviewers independently completed a standardised data extraction form and critically evaluated identified documents using a validated appraisal tool for empirical studies with varied methodologies. Results: A total of 19 studies of variable quality were identified. None were randomised or case-controlled trials. Studies incorporating service user/carer perspectives highlighted the need to tackle stigma and provide accessible, age-appropriate services. Parents/carers wanted more involvement with AMHS. Transitional care provision was considered patchy and often not prioritised within mental health services. There was no clear evidence of superior effectiveness of any particular model. Conclusions: High-quality evidence of transitional care models is lacking. Data broadly support the development of programmes that address the broader transitional care needs of ‘emerging adults’ and their mental health needs but further evaluation is necessary. Developing robust transitional mental health care will require the policy–practice gap to be addressed and development of accessible, acceptable, responsive, age-appropriate provision.
Bloch Surface Wave Photonic Device Fabricated by Femtosecond Laser Polymerisation Technique

We applied femtosecond laser polymerisation technique to fabricate a novel Bloch surface wave integrated photonic device with a compact coupling scheme. The device consisted of a waveguide, coupling and decoupling gratings and focusing and defocusing triangles. We manufactured an array of devices with varying geometrical parameters of waveguide. Excitation and propagation of Bloch surface wave waveguide modes were studied by direct and back focal plane imaging. The obtained results prove that the maskless and flexible femtosecond laser polymerisation technique may be applied for fabrication of Bloch-surface-wave based integrated photonics.
Redesign of the ECT Test for Mode III Delamination Testing. Part I: Finite Element Analysis

A series of finite element analyses (Part I) and tests (Part II) on various modifications of the edge crack torsion (ECT) test have been conducted in an effort to render the test more suitable for characterizing mode III delamination in laminated composites. To this end, two ECT specimen configurations were considered. The first configuration involves loading the specimen at a single location, and the second configuration involves a symmetric double load point application. Investigations were conducted on the effect of specimen overhang, along the length and width direction, on the inferred strain energy release rate distributions across the delamination front. Stress distributions in the vicinity of the delamination front were used to infer the corresponding modes I, II, and III strain energy release rate distributions. Results indicate the single and double loading configurations exhibiting similar stress distributions along the delamination front. Specimens with short crack lengths and small amounts of overhang yielded the most uniform distribution of mode III loading along the delamination front.
Transporter-Guided Delivery of Nanoparticles to Improve Drug Permeation across Cellular Barriers and Drug Exposure to Selective Cell Types

Targeted nano-drug delivery systems conjugated with specific ligands to target selective cell-surface receptors or transporters could enhance the efficacy of drug delivery and therapy. Transporters are expressed differentially on the cell-surface of different cell types, and also specific transporters are expressed at higher than normal levels in selective cell types under pathological conditions. They also play a key role in intestinal absorption, delivery via non-oral routes (e.g., pulmonary route and nasal route), and transfer across biological barriers (e.g., blood–brain barrier and blood–retinal barrier. As such, the cell-surface transporters represent ideal targets for nano-drug delivery systems to facilitate drug delivery to selective cell types under normal or pathological conditions and also to avoid off-target adverse side effects of the drugs. There is increasing evidence in recent years supporting the utility of cell-surface transporters in the field of nano-drug delivery to increase oral bioavailability, to improve transfer across the blood–brain barrier, and to enhance delivery of therapeutics in a cell-type selective manner in disease states. Here we provide a comprehensive review of recent advancements in this interesting and important area. We also highlight certain key aspects that need to be taken into account for optimal development of transporter-assisted nano-drug delivery systems.