Miracle-dz/UniFAR-FTUs huggingface dataset:
Nrc Publications Archive Archives Des Publications Du Cnrc New Elisa Approach Based on Coiled-coil Interactions New Elisa Approach Based on Coiled-coil Interactions

L'accès à ce site Web et l'utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D'UTILISER CE SITE WEB. Access and use of this website and the material on it are subject to the Terms and Conditions set forth at This publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below. / Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous. The de novo designed heterodimeric E/K coiled-coil system has been previously demonstrated to be an excellent capture/dimerization system applicable to various needs in both biotechnology and pharmaceutical fields. Those include controlled protein dimerization, capture, purification and Western-blot detection. We here report the development of a new generation of ELISA test based on coiled-coil interactions for the direct quantitation of coil-tagged epidermal growth factor (EGF). The new approach was evaluated for its specificity, plate storability and reusability as well as for convenience when compared to commercially available systems. Our results show a similar affinity/sensitivity to standard capturing antibody-based ELISA systems and an improved affinity/ sensitivity when compared to the commercially available Ni-NTA capture system. The E/K coiled-coil ELISA system was validated with respect to recovery, intra-and inter-assay variations. The practical working range was estimated to be between 5.2 and 34,000 pM. Furthermore, the storability and reusability of the plates was greater than the two aforementioned systems, suggesting that the E/K coiled-coil system is a good alternative to traditional tags such as poly-histidine for the development of ELISA tests aiming at quantitating coil-tagged proteins. 1. Introduction α-helical coiled-coil is an oligomerization motif being naturally present in a wide variety of proteins such as transcription factors, motor and viral fusion proteins (Burkhard et al., 2001). In fact, protein data base analysis revealed that approximately 10% of all proteins contain coiled-coil domains, therefore making it one of the most abundant motif found in proteins (Mewes et al., 2000; Walshaw and Woolfson, 2001). Coiled-coil motif results from the wrapping of two or more amphipathic α-helices around each other in a left handed supercoil fashion; the helices being aligned in either a parallel or anti-parallel manner (Lupas, 1996). Each peptide involved in this domain …
Sandpile Model on Sierpinski Gasket Fractal

We investigate the sandpile model on the two--dimensional Sierpinski gasket fractal. We find that the model displays novel critical behavior, and we analyze the distribution functions of avalanche sizes, lifetimes and topplings and calculate the associated critical exponents $\tau = 1.51 \pm 0.04$, $\alpha = 1.63 \pm 0.04$ and $\mu = 1.36 \pm 0.04$. The avalanche size distribution shows power law behavior modulated by logarithmic oscillations which can be related to the discrete scale invariance of the underlying lattice. Such a distribution can be formally described by introducing a complex scaling exponent ${\tau}^{*} \equiv \tau + i \delta$, where the real part $\tau$ corresponds to the power law and the imaginary part $\delta$ is related to the period of the logarithmic oscillations.
Association of NAD(P)H oxidase p22 phox gene variation with advanced carotid atherosclerosis in Japanese type 2 diabetes.

OBJECTIVE To evaluate the association between the C242T polymorphism of the p22 phox gene, an essential component of NAD(P)H oxidase in the vasculature, with intima-media thickness (IMT) of the carotid artery and risk factors for atherosclerosis in type 2 diabetic subjects. RESEARCH DESIGN AND METHODS C242T polymorphism of the p22 phox gene was detected by polymerase chain reaction-restriction fragment-length polymorphism in 200 Japanese type 2 diabetic subjects and 215 nondiabetic subjects. We examined the association with this mutation and carotid atherosclerosis as well as the patients' clinical characteristics and the level of 8-hydroxy-2'deoxyguanosine (8-OHdG) as an index of oxidative DNA damage. RESULTS The diabetic subjects with the TC+TT genotypes displayed a significantly lower average IMT (1.13 +/- 0.31 vs. 1.31 +/- 0.34 mm; P = 0.0099) and a not significantly lower serum 8-OHdG level than those with the CC genotype, despite no difference in the risk factors. Stepwise multiple regression analysis showed that the risk factors for increased IMT in the diabetic subjects were systolic blood pressure (P = 0.0042) and p22 phox CC genotype (P = 0.0151). In nondiabetic subjects, the average IMT of the TC+TT group was not different from that of the CC group (0.85 +/- 0.14 vs. 0.94 +/- 0.30 mm, P = 0.417). Fasting plasma insulin concentration (41.4 +/- 15.6 vs. 64.2 +/- 59.4 pmol/l, P = 0.0098) and insulin resistance index of homeostasis model assessment (HOMA-R) (1.58 +/- 0.66 vs. 2.60 +/- 2.56, P = 0.0066) were significantly lower in the TC+TT group than in the CC group. CONCLUSIONS These results show that the C242T mutation in the p22 phox gene is associated with progression of asymptomatic atherosclerosis in the subjects with type 2 diabetes and is also associated with insulin resistance in nondiabetic subjects.
The Degree of Applying the Theoretical Frameworks of Child-Raising Speciall Courses in the Field of Training among the Female Students of Princess Alia University College

هدفت الدراسة إلى التعرف على درجة تطبيق الأطر النظرية لمساقات تخصص تربية الطفل في التدريب الميداني لدى طالبات كلية الأميرة عالية الجامعية، وقد تكون مجتمع الدراسة من فئتين هما: أعضاء هيئة التدريس المتابعون لفعاليات البرنامج وعددهم (42) مدرسا، والمشرفون التربويون الذين يتابعون أداء المدارس المتعاونة، التي يتم فيها تطبيق البرنامج وعددهم (36) مشرفا تربويا، وقد أجريت الدراسة على الطالبات الملتحقات بالبرنامج وعددهن (137) طالبة في العام الجامعي (2012-2013). وقد طور الباحثان أداة للإجابة عن أسئلة الدراسة، تم التحقق من إجراءات صدقها وثباتها. وأشارت النتائج إلى وجود مستوى عال في الكفايات المهنية لطالبات تخصص تربية الطفل في كلية الأميرة عالية من وجهة نظر أعضاء هيئة التدريس، في حين جاء هذا المستوى متوسطا من وجهة نظر المشرفين التربوين، كما أشارت النتائج إلى فروق فردية ذات دلالة إحصائية تعزى لأثر المسمى الوظيفي، وجاءت الفروق لصالح أعضاء هيئة التدريس. وفي ضوء نتائج الدراسة أوصى الباحثان بعدد من التوصيات من أهمها؛التنسيق بين وزارة التربية، وكلية الأميرة عالية الجامعية، للانطلاق من رؤية مشتركة، وواضحة المعالم في الأهداف، والإجراءات، وعمليات التقويم، واعتماد علامة محددة لمادة التدريب الميداني.
A logical toolbox for knowledge approximation

It is well-known that the logicist approach to agency is confronted with both epistemological and heuristic problems. On the one hand, the agent's model must be logically adequate: it must provide us a clear picture of what the agent is, and is not, able to deduce from its background knowledge. On the other hand, the agent's program must be adequate in practice: it must generate useful conclusions from input data and given the computational resources that are actually available. In actuality, the agent's need for heuristic adequacy has strong epistemological consequences. Based on this argument, this paper proposes a framework which is based on the paradigm of knowledge approximation and that is flexible enough to incorporate heuristic strategies used in satisfiability algorithms. The framework is used as a "logical toolbox" for modelling resource-bounded agents that have different operational means at their disposal to approximate knowledge. The toolbox consists in a family of relative relevance logics which are semantically founded on the notion of resource and that include interesting features, such as incremental reasoning and dual approximations.
Classical arithmetic logic unit embedded on reversible/quantum circuit

Reversible circuit dissipates less heat than irreversible circuit. A promising use of reversible circuit may be embedding of reversible circuits in irreversible general purpose computers to allow low-power design. In this paper, we embed an n-bit classical ALU on reversible circuit, which can perform addition, subtraction, EXOR, EXNOR, AND, NAND, OR, NOR, and NOT operations on n-bit data. The quantum realization of our n-bit ALU requires 27n — 10 primitive quantum gates with quantum circuit width of 4n + 5. The known reversible n-bit ALU capable of performing only mod 2n addition, subtraction, negative subtraction, EXOR, and no-operation requires 22n — 10 primitive quantum gates with quantum circuit width of 2n + 5. With a marginal increase of quantum primitive gate count and nearly doubling the quantum circuit width, our ALU implements a larger set of operation needed for general purpose computing.
First-in-human evaluation of a hexon chimeric adenovirus vector expressing HIV-1 Env (IPCAVD 002).

BACKGROUND We report the first-in-human safety and immunogenicity assessment of a prototype hexon chimeric adenovirus (Ad) serotype 5 (Ad5) vector containing the hexon hypervariable regions of Ad serotype 48 (Ad48) and expressing human immunodeficiency virus (HIV) type 1 EnvA. METHODS Forty-eight Ad5 and Ad48 seronegative, HIV-uninfected subjects were enrolled in a randomized, double-blind, placebo-controlled, dose escalation phase 1 study. Four groups of 12 subjects received 10(9) to 10(11) viral particles (vp) of the Ad5HVR48.EnvA. 01 vaccine (n = 10 per group) or placebo (n = 2 per group) at week 0 or weeks 0, 4, and 24. Safety and immunogenicity were assessed. RESULTS Self-limited reactogenicity was observed after the initial immunization in the highest (10(11) vp) dose group. Responses in vaccinees included Ad48 neutralizing antibody (nAb) titers higher than Ad5 nAb titers, EnvA-specific enzyme-linked immunosorbent assay titers, and EnvA-specific enzyme-linked immunospot assay responses, and these responses generally persisted at week 52. At week 28 in the 10(9), 10(10), and 10(11) vp 3-dose groups, geometric mean EnvA enzyme-linked immunosorbent assay titers were 5721, 10 929, and 3420, respectively, and Ad48 nAb titers were a median of 1.7-fold higher than for Ad5. CONCLUSIONS Ad5HVR48.ENVA. 01 was safe, well tolerated, and immunogenic at all doses tested. Vector-elicited nAb responses were greater for Ad48 than Ad5, confirming that Ad-specific nAbs in humans are primarily, but not exclusively, directed against the hexon hypervariable regions. Clinical Trials Registration. NCT00695877.
Learning to Win Process-Control Games Watching Game-Masters

The present paper focuses on some interesting classes of process-control games, where winning essentially means successfully controlling the process. A master for one of these games is an agent who plays a winning-strategy. In this paper we investigate situations, in which even a complete model (given by a program) of a particular game does not provide enough information to synthesize -- even in the limit -- a winning strategy. However, if in addition to getting a program, a machine may also watch masters play winning strategies, then the machine is able to learn in the limit a winning strategy for the given game. Studied are successful learning from arbitrary masters and from pedagogically useful selected masters. It is shown that selected masters are strictly more helpful for learning than are arbitrary masters. Both for learning from arbitrary masters and for learning from selected masters, though, there are cases where one can learn programs for winning strategies from masters but not if one is required to learn a program for the master's strategy itself. Both for learning from arbitrary masters and for learning from selected masters, one can learn strictly more watching m + 1 masters than one can learn watching only m. Lastly a simulation result is presented where the presence of a selected master reduces the complexity from infinitely many semantic mind changes to finitely many syntactic ones.
Surveying the Relationship between Psychological Disorders and Spending Time on Facebook

Introduction: This research is a descriptive and correlational research which has been done with the purpose of surveying the relationship between mental disorders and the amount of time that people spend on Facebook. 147 members out of Facebook active users were chosen randomly. Then epidemiology questionnaire of mental disorders (SCL-90-R) was used to collect data. Data analysis was done using Spearman correlation coefficient and multiple regression. The results showed that there was a significant relation between characters such as aggression, obsession, psychosis, paranoia, depression and using Facebook (p < 0.05), but this relation was not significant in anxiety, interpersonal sensitivity, physical complaints and phobia. Furthermore, this relationship was significant regarding to subjects’ gender and marital status (p < 0.05), but there was no relationship regarding to their age.
The Influence of the Solar Cycle and QBO on the Late-Winter Stratospheric Polar Vortex

Abstract A statistical analysis of 51 years of NCEP–NCAR reanalysis data is conducted to isolate the separate effects of the 11-yr solar cycle (SC) and the equatorial quasi-biennial oscillation (QBO) on the Northern Hemisphere (NH) stratosphere in late winter (February–March). In a four-group [SC maximum (SC-max) versus minimum (SC-min) and east-phase versus west-phase QBO] linear discriminant analysis, the state of the westerly phase QBO (wQBO) during SC-min emerges as a distinct least-perturbed (and coldest) state of the stratospheric polar vortex, statistically well separated from the other perturbed states. Relative to this least-perturbed state, the SC-max and easterly QBO (eQBO) each independently provides perturbation and warming as does the combined perturbation of the SC-max–eQBO. All of these results (except the eQBO perturbation) are significant at the 95% confidence level as confirmed by Monte Carlo tests; the eQBO perturbation is marginally significant at the 90% level. This observational res.
Teenage childbearing: young sole mothers challenge the stereotypes

ABSTRACT Teenage sole mothers in receipt of welfare benefits are seriously affected by systematic and radical restructuring of Aotearoa/New Zealand’s state welfare system since 2008. With few exceptions, the changes oblige beneficiaries to prioritise obtaining paid work. To address questions of how welfare changes and socio-cultural context in Aotearoa New Zealand are experienced by teenage mothers we conducted a narrative study of 10 young women’s stories of sole mothering as beneficiaries. Purposeful sampling was used to recruit young mothers who were attending, or had recently attended a specialist school for teen parents. Thematic analysis of interview narratives represented experiences of financial and social deprivation, negative stereotyping, stigma and resistance. In contrast to the government’s explicit goal of getting welfare recipients into paid employment, the teenage mothers in our study placed full-time mothering as their principal duty. They also acknowledged the material and social advantages of entering paid employment, and were committed to working but only when their child was ‘settled and ready’ – a decision they understood to be their responsibility.
Congestion-Aware Distributed Network Selection for Integrated Cellular and Wi-Fi Networks

Intelligent network selection plays an important role in achieving an effective data offloading in the integrated cellular and Wi-Fi networks. However, previously proposed network selection schemes mainly focused on offloading as much data traffic to Wi-Fi as possible, without systematically considering the Wi-Fi network congestion and the ping-pong effect, both of which may lead to a poor overall user quality of experience. Thus, in this paper, we study a more practical network selection problem by considering both the impacts of the network congestion and switching penalties. More specifically, we formulate the users' interactions as a Bayesian network selection game (NSG) under the incomplete information of the users' mobilities. We prove that it is a Bayesian potential game and show the existence of a pure Bayesian Nash equilibrium that can be easily reached. We then propose a distributed network selection (DNS) algorithm based on the network congestion statistics obtained from the operator. Furthermore, we show that computing the optimal centralized network allocation is an NP-hard problem, which further justifies our distributed approach. Simulation results show that the DNS algorithm achieves the highest user utility and a good fairness among users, as compared with the on-the-spot offloading and cellular-only benchmark schemes.
Text Stemming

Stemming is a process in which the variant word forms are mapped to their base form. It is among the basic text pre-processing approaches used in Language Modeling, Natural Language Processing, and Information Retrieval applications. In this article, we present a comprehensive survey of text stemming techniques, evaluation mechanisms, and application domains. The main objective of this survey is to distill the main insights and present a detailed assessment of the current state of the art. The performance of some well-known rule-based and statistical stemming algorithms in different scenarios has been analyzed. In the end, we highlighted some open issues and challenges related to unsupervised statistical text stemming. This research work will help the researchers to select the most suitable text stemming technique in a specific application and will also serve as a guide to identify the areas that need attention from the research community.
Mechanics and refractive power optimization of tunable acoustic gradient lenses

Tunable acoustic gradient index (TAG) lenses create tunable multiscale Bessel beams. These lenses are fluid-filled cylindrical cavities within which an acoustic radial standing wave is excited. This standing wave modulates the density, and thereby the refractive index within the lens. Spatial gradients in the refractive index can be used for lensing. A predictive model for the steady-state fluid mechanics behind TAG lenses driven with a sinusoidal voltage signal is presented here. The model covers inviscid and viscous regimes in both the resonant and off-resonant cases. The density fluctuations from the fluidic model are related to refractive index fluctuations. The entire model is then analyzed to determine the optimal values of lens design parameters for greatest lens refractive power. These design parameters include lens length, radius, static refractive index, fluid viscosity, sound speed, and driving frequency and amplitude. It is found that long lenses filled with a fluid of high refractive index an.
Using routine data to improve palliative and end of life care

Palliative and end of life care is essential to healthcare systems worldwide, yet a minute proportion of research funding is spent on palliative and end of life care research. Routinely collected health and social care data provide an efficient and useful opportunity for evaluating and improving care for patients and families. There are excellent examples of routine data research in palliative and end of life care, but routine data resources are widely underutilised. We held four workshops on using routinely collected health and social care data in palliative and end of life care. Researchers presented studies from the UK, USA and Europe. The aim was to highlight valuable examples of work with routine data including work with death registries, hospital activity records, primary care data and specialist palliative care registers. This article disseminates that work, describes the benefits of routine data research and identifies major challenges for the future use of routine data, including; access to data, improving data linkage, and the need for more palliative and end of life care specific data.
Neural Coding of Sound Frequency by Cricket Auditory Receptors

Crickets provide a useful model to study neural processing of sound frequency. Sound frequency is one parameter that crickets use to discriminate between conspecific signals and sounds made by predators, yet little is known about how frequency is represented at the level of auditory receptors. In this paper, we study the physiological properties of auditory receptor fibers (ARFs) by making single-unit recordings in the cricket Teleogryllus oceanicus. Characteristic frequencies (CFs) of ARFs are distributed discontinuously throughout the range of frequencies that we investigated (2–40 kHz) and appear to be clustered around three frequency ranges (≤5.5, 10–12, and ≥18 kHz). A striking characteristic of cricket ARFs is the occurrence of additional sensitivity peaks at frequencies other than CFs. These additional sensitivity peaks allow crickets to detect sound over a wide frequency range, although the CFs of ARFs cover only the frequency bands mentioned above. To the best of our knowledge, this is the first example of the extension of an animal’s hearing range through multiple sensitivity peaks of auditory receptors.
Are there different kinds of content

The cup from which I am drinking water now is yellow, and I know that it is. Why does my belief that the cup is yellow count as knowledge? Presumably, the answer must involve some reference to my current perceptual experience: I see the cup, and I see that it is yellow. What is it for me to see that the cup is yellow? The obvious answer would seem to be that it is for me to stand in a certain relation—namely, the relation expressed by the verb ‘to see’—to a proposition, namely, the proposition that the cup is yellow: Perception, that is to say, is a kind of propositional attitude, like belief, though it is also different from belief in many ways. So my seeing that the cup is yellow, being a kind of propositional attitude I take towards that proposition, can count as my reason for believing that the cup is yellow. I’ve thus got a reason for that belief, and it’s a good one. No sort of inference from a prior judgement about my experience is necessary. Rather, I need only import the content of my perceptual state into cognition to believe it. If one could accept that much, then—though there would no doubt be many problems left to discuss—it would make the question how perceptual experience justifies perceptual beliefs significantly more tractable. But the problem, as I see it, is that accepting that much threatens to impose high costs. If the ‘importation’ model of perceptual justification is to be extended to all perceptually justified beliefs, then every concept that figures in a belief that is perceptually justifiable for a given subject must also be able to figure in the content of her perceptual experience: Only what the subject can conceptualize can play any justificatory role for her. It does not, of course, follow that there is nothing in the content of one’s current perceptual experience that one cannot now conceptualize. But even if there is, it can play no role in thought: It cannot so much as figure in thought. A fortiori, non-conceptual elements of experience, if such there be, can play no role in one’s decisions about what to believe or, more generally, one might conjecture, in any rational process. Elements of one’s current perceptual experience that cannot be conceptualized would be of no significance for the thinking subject. Or, as Kant put it, in a famous passage: