Miracle-dz/UniFAR-FTUs huggingface dataset:
Deletion of Individual Ku Subunits in Mice Causes an NHEJ-Independent Phenotype Potentially by Altering Apurinic/Apyrimidinic Site Repair

Ku70 and Ku80 form a heterodimer called Ku that forms a holoenzyme with DNA dependent-protein kinase catalytic subunit (DNA-PKCS) to repair DNA double strand breaks (DSBs) through the nonhomologous end joining (NHEJ) pathway. As expected mutating these genes in mice caused a similar DSB repair-defective phenotype. However, ku70-/- cells and ku80-/- cells also appeared to have a defect in base excision repair (BER). BER corrects base lesions, apurinic/apyrimidinic (AP) sites and single stand breaks (SSBs) utilizing a variety of proteins including glycosylases, AP endonuclease 1 (APE1) and DNA Polymerase β (Pol β). In addition, deleting Ku70 was not equivalent to deleting Ku80 in cells and mice. Therefore, we hypothesized that free Ku70 (not bound to Ku80) and/or free Ku80 (not bound to Ku70) possessed activity that influenced BER. To further test this hypothesis we performed two general sets of experiments. The first set showed that deleting either Ku70 or Ku80 caused an NHEJ-independent defect. We found ku80-/- mice had a shorter life span than dna-pkcs-/- mice demonstrating a phenotype that was greater than deleting the holoenzyme. We also found Ku70-deletion induced a p53 response that reduced the level of small mutations in the brain suggesting defective BER. We further confirmed that Ku80-deletion impaired BER via a mechanism that was not epistatic to Pol β. The second set of experiments showed that free Ku70 and free Ku80 could influence BER. We observed that deletion of either Ku70 or Ku80, but not both, increased sensitivity of cells to CRT0044876 (CRT), an agent that interferes with APE1. In addition, free Ku70 and free Ku80 bound to AP sites and in the case of Ku70 inhibited APE1 activity. These observations support a novel role for free Ku70 and free Ku80 in altering BER.
The Alliance Negotiation Scale: Psychometric construction and preliminary reliability and validity analysis

Abstract Current measures of the working alliance tend to emphasize the degree of agreement or collaboration between patient and therapist about therapeutic tasks and goals. There are, however, both theoretical and empirical grounds to suggest that the degree to which patient and therapist are able to constructively negotiate disagreements about tasks and goals is another important dimension of the alliance. The present study outlines the preliminary development and evaluation of a measure to operationalize this dimension. This measure, the Alliance Negotiation Scale (ANS), is a 12-item patient self-report instrument. Exploratory factor analysis and expert ratings informed the development of the scale, which consists of two distinct factors that demonstrate good internal consistency. Correlations between these factors and the working alliance offer preliminary support for its construct validity. A working version of the ANS is presented and the need for further validation is discussed.
Postoperative residual curarization: clinical observation in the post-anesthesia care unit.

BACKGROUND Neuromuscular blocking agents (NMBAs) are widely used during the induction and maintenance of anesthesia. Postoperative residual curarization (PORC) following the use of NMBAs still occurs even though intermediate-acting NMBAs were used. Train-of-four (TOF) stimulation is used to quantify the degree of neuromuscular blockade. The TOF ratio of 0.7 in the adductor pollicis muscle was associated with normal respiratory function. Pharyngeal function returned to normal while the TOF ratio reached 0.9. The aim of this study was to survey the PORC in the post-anesthesia care unit (PACU). METHODS In this observational study, 308 patients who received general anesthesia with NMBAs were enrolled. Residual curarization was evaluated using the TOF-Watch acceleromyograph upon arrival in the PACU. Three consecutive TOF stimulations were applied and recorded at 15-second intervals. Two thresholds of TOF ratios (0.9 and 0.7) were used to assess the presence of residual curarization. RESULTS TOF ratios less than 0.7 and 0.9 were observed in 15 (5%), and 97 (31%) patients, respectively. The differences of the TOF ratios between the male and female patients were significant (p = 0.014). In terms of weight, the differences between the patients with ratios > 0.9 and < or = 0.9 were significant (p = 0.013). There were 67, 49, 15, and three patients who received reversal medication in the > 0.9 group, < or = 0.9 and > 0.7 group, and < or = 0.7 group, respectively. The differences of the TOF ratios between the patients who received and those who did not receive reversal medication were not significant (p = 0.91). CONCLUSION PORC is still a clinical problem in the modern PACU. Objective neuromuscular monitoring needs to be performed to ensure patient safety.
Functional outcomes after radiotherapy or laser surgery in early glottic carcinoma: A systematic review

Early glottic carcinoma is treated with laser surgery or radiotherapy, but which treatment has better functional outcomes is unclear. This systematic review compared functional outcomes (voice, swallowing, quality of life [QOL]) in more extended T1a and limited T2 tumors (1) between treatments and (2) between greater and lesser laser resections.
Social and gender variation in the prevalence, presentation and general practitioner provisional diagnosis of chest pain

OBJECTIVES To describe the prevalence of Rose angina and non-exertional chest pain in men and women in socioeconomically contrasting areas; to describe the proportions of men and women who present with the symptom of chest pain and who receive a provisional general practitioner diagnosis of coronary heart disease; to assess the effects of gender and deprivation. DESIGN Two random general population samples in socially contrasting areas were surveyed using the Rose angina questionnaire: the case notes of people identified with chest pain were reviewed. SETTING Glasgow conurbation. PARTICIPANTS 1107 men and women, aged 45–64, with chest pain. OUTCOME MEASURES Prevalence of Rose angina and non-exertional chest pain; the proportions who had presented with chest pain and received a general practitioner's provisional diagnosis of coronary heart disease. RESULTS There was no difference between social groups in the prevalence of all chest pain but a greater proportion of those in deprived groups had Rose angina and a greater proportion of these had the more severe grade. The proportion of people who had presented with chest pain was higher among socioeconomically deprived groups but there was no difference in the proportions receiving a general practitioner provisional diagnosis of coronary heart disease. Men were more likely to present with chest pain than women and were more likely to receive a provisional general practitioner diagnosis of coronary heart disease. CONCLUSIONS No evidence was found of social differences in patient presentation or general practitioner diagnosis that might explain reported variations in uptake of cardiology services. In contrast, gender variation may originate in part from differences in patient presentation and general practitioner diagnosis. Further investigation of socioeconomic variations in uptake of cardiology services should focus later in the care pathway, on general practitioner referral patterns and clinical decisions taken in secondary care.
Predictive Model for Pitting Corrosion in Buried Oil and Gas Pipelines

Abstract A predictive model for pitting corrosion in buried pipelines is proposed. The model takes into consideration the chemical and physical properties of the soil and pipe to predict the time dependence of pitting depth and rate. Maximum pit depths were collected together with soil and pipe data at more than 250 excavation sites over a three-year period. The time dependence of the maximum pit depth was modeled as dmax = κ(t − t0)ν, where t is the exposure time, t0 is the pit initiation time, and κ and ν are the pitting proportionality and exponent parameters, respectively. A multivariate regression analysis was conducted with dmax as the dependent variable and the pipeline age, and the soil and pipe properties as the independent variables. The dependence of κ and ν on the predictor variables was found for the three soil textural classes identified in this study: clay, clay loam, and sandy clay loam. The proportionality parameter κ was found to be primarily influenced by the redox potential, pH value, .
Efficacy of antineoplastic treatment is associated with the use of antibiotics that modulate intestinal microbiota

ABSTRACT Reduced anticancer efficacy of cyclophosphamide and platinum salts has been reported in animals treated with anti-Gram-positive antibiotics. These effects were related to translocation of Gram-positive bacteria during mucositis with subsequent induction of cytotoxic oxygen reactive species and tumor invasion by pathogenic Th17 cells. To assess these hypotheses in a clinical setting, we identified patients receiving cyclophosphamide for chronic lymphocytic leukemia (CLL) and cisplatin for relapsed lymphoma. Data originated from the CLL8 trial (NCT00281918) and the Cologne Cohort of Neutropenic Patients (NCT01821456). Relevant antibiotics were defined as compounds with primary activity against Gram-positive bacteria. We evaluated their impact on response, progression-free survival (PFS) and overall survival (OS) by Kaplan–Meier methodology and Cox proportional hazards regression analysis. Among 800 available CLL patients, those receiving anti-Gram-positive antibiotics (n = 45/800) achieved a significantly lower overall response rate (OR 74.3% vs. 90.2%, p = 0.007). Patients with anti-Gram-positive antibiotics progressed significantly earlier, had a reduced OS (median PFS 14.1 vs. 44.1 mo, p < 0.001; median OS 56.1 vs. 91.7 mo, p < 0.001) and multivariate analysis showed that administration of anti-Gram-positive antibiotic treatment was independently associated with reduced PFS (Hazard ratio (HR) 2.090, p = 0.001) and OS (HR 2.966, p < 0.001). Of 122 patients with relapsed lymphoma, those treated with anti-Gram-positive antibiotics (n = 21/122) achieved a significantly lower OR rate (70.3% vs. 42.9%, p = 0.016). Patients with anti-Gram-positive antibiotics progressed significantly earlier than others (median PFS 2.3 vs. 11.5 mo, p = 0.001). As for multivariate analysis, the use of anti-Gram-positive antibiotics was independently associated with reduced PFS (HR 2.237, p = 0.012) and OS (HR 7.831, p < 0.001). Our data supports a potential negative impact of anti-Gram-positive antibiotics on the anticancer activity of cyclophosphamide and cisplatin in a clinical setting.
A Posterior Centre Establishes and Maintains Polarity of the Caenorhabditis elegans Embryo by a Wnt-Dependent Relay Mechanism

Cellular polarity is a general feature of animal development. However, the mechanisms that establish and maintain polarity in a field of cells or even in the whole embryo remain elusive. Here we provide evidence that in the Caenorhabditis elegans embryo, the descendants of P1, the posterior blastomere of the 2-cell stage, constitute a polarising centre that orients the cell divisions of most of the embryo. This polarisation depends on a MOM-2/Wnt signal originating from the P1 descendants. Furthermore, we show that the MOM-2/Wnt signal is transduced from cell to cell by a relay mechanism. Our findings suggest how polarity is first established and then maintained in a field of cells. According to this model, the relay mechanism constantly orients the polarity of all cells towards the polarising centre, thus organising the whole embryo. This model may also apply to other systems such as Drosophila and vertebrates.
A Treatment Algorithm for Neuropathic (Charcot) Midfoot Deformity*

Forty-nine feet in 47 patients with midfoot neuropathic foot deformity were referred for care to a comprehensive foot salvage clinic and followed for an average of 3.6 years. Twenty-three initially presented without open ulcers. Two underwent elective Syme's ankle disarticulation amputation, and the others were kept ambulatory with a combination of periods of nonweightbearing cast immobilization, accommodative extra-depth shoes with custom orthotics, and ankle-foot orthoses. Twenty-six of the feet initially presented for care with open ulcers and/or chronic osteomyelitis. Twenty-two of these patients underwent 32 surgical procedures. Sixteen underwent debridement of the infected bone and surrounding soft tissues. Excision of large, nonaccommodative boney prominence, termed ex-ostectomy, was performed in eight. Partial excision of the deformed midfoot combined with boney stabilization and attempted arthrodesis, termed partial tarsectomy, were performed in seven. All surgical patients were managed postoperatively with long-term custom accommodative bracing. Follow-up at an average of 3.6 years revealed that all but one of the patients remained ambulatory. Six walk with accommodative shoe gear and persistent stable chronic open ulcers. None required below-knee amputation. Five amputations were performed, three at the Syme's ankle disarticulation level, one at the Chopart's hind-foot level, and one at the mid-foot level. Neuropathic midfoot deformity is a disabling disorder that requires thorough patient education and compliance, prolonged palliative monitoring and care, accommodative orthotic shoe management, and selective surgery. It can markedly limit the patient's walking ability and independence. With careful monitoring and selective surgery to maintain a plantigrade foot, foot salvage and walking ability can be maintained. A treatment algorithm is presented to guide patient care.
Low voltage ridge guide LiNbO3 Mach-Zehnder modulator

Mach-Zehnder type interferometric (MZI) modulators based on lithium niobate (LiNbO3) are key components in today's optical communication systems. Despite their excellent properties, future optical networks will require devices of lower drive voltage (Vπ) and even higher bandwidth capability (40 GHz or higher). In particular, values of Vπ ≤ 2 V would allow using much cheaper driving electronics. This was our motivation to develop MZI modulators with two novel characteristics: (i) the whole interferometer including Y-junctions consists of low loss ridge waveguides of strong light confinement [1]; (ii) a special electrode structure — designed for high bandwidth operation - enables low drive voltages.
Autotagger: A Model for Predicting Social Tags from Acoustic Features on Large Music Databases

Abstract Social tags are user-generated keywords associated with some resource on the Web. In the case of music, social tags have become an important component of “Web 2.0” recommender systems, allowing users to generate playlists based on use-dependent terms such as chill or jogging that have been applied to particular songs. In this paper, we propose a method for predicting these social tags directly from MP3 files. Using a set of 360 classifiers trained using the online ensemble learning algorithm FilterBoost, we map audio features onto social tags collected from the Web. The resulting automatic tags (or autotags) furnish information about music that is otherwise untagged or poorly tagged, allowing for insertion of previously unheard music into a social recommender. This avoids the “cold-start problem” common in such systems. Autotags can also be used to smooth the tag space from which similarities and recommendations are made by providing a set of comparable baseline tags for all tracks in a recommender system. Because the words we learn are the same as those used by people who label their music collections, it is easy to integrate our predictions into existing similarity and prediction methods based on web data.
Analytical Determination of the Distribution of Polishing Time over the Surface of Polished Tiles

Several kinds of glossiness pattern can be seen on the surface of porcelain stoneware tiles right after the polishing process, as a function of the kinematics performed by the polishing heads. For the newest generation of industrial polishing trains, where a transverse oscillation is included, there is still a great need for literature about the resulting patterns. This paper intends to find the spatial distribution of time under polishing analytically using the kinematics equations involved in the polishing process. The measured values of glossiness collected from three polished tiles are also presented. The importance of adopting a good kinematics for the polishing process has been highlighted, and the equations developed herein are useful tools for further attempts at optimizing the polishing process.
Acute Exercise-Induced Mitochondrial Stress Triggers an Inflammatory Response in the Myocardium via NLRP3 Inflammasome Activation with Mitophagy

Increasing evidence has indicated that acute strenuous exercise can induce a range of adverse reactions including oxidative stress and tissue inflammation. However, little is currently known regarding the mechanisms that underlie the regulation of the inflammatory response in the myocardium during acute heavy exercise. This study evaluated the mitochondrial function, NLRP3 inflammasome activation, and mitochondrial autophagy-related proteins to investigate the regulation and mechanism of mitochondrial stress regarding the inflammatory response of the rat myocardium during acute heavy exercise. The results indicated that the mitochondrial function of the myocardium was adaptively regulated to meet the challenge of stress during acute exercise. The exercise-induced mitochondrial stress also enhanced ROS generation and triggered an inflammatory reaction via the NLRP3 inflammasome activation. Moreover, the mitochondrial autophagy-related proteins including Beclin1, LC3, and Bnip3 were all significantly upregulated during acute exercise, which suggests that mitophagy was stimulated in response to the oxidative stress and inflammatory response in the myocardium. Taken together, our data suggest that, during acute exercise, mitochondrial stress triggers the rat myocardial inflammatory response via NLRP3 inflammasome activation and activates mitophagy to minimize myocardial injury.
Similarity search without tears: the OMNI-family of all-purpose access methods

Designing a new access method inside a commercial DBMS is cumbersome and expensive. We propose a family of metric access methods that are fast and easy to implement on top of existing access methods, such as sequential scan, R-trees and Slim-trees. The idea is to elect a set of objects as foci, and gauge all other objects with their distances from this set. We show how to define the foci set cardinality, how to choose appropriate foci, and how to perform range and nearest-neighbor queries using them, without false dismissals. The foci increase the pruning of distance calculations during the query processing. Furthermore we index the distances from each object to the foci to reduce even triangular inequality comparisons. Experiments on real and synthetic datasets show that our methods match or outperform existing methods. They are up to 10 times faster, and perform up to 10 times fewer distance calculations and disk accesses. In addition, it scales up well, exhibiting sub-linear performance with growing database size.
An Efficient Approach to Correct the Signals and Generate High-Resolution Quadrature Pulses for Magnetic Encoders

A magnetic encoder (ME) is a kind of sinusoidal encoder using magnetic effects that is currently utilized in many industrial control systems because it has many advantageous characteristics: low cost, simple structure, works in harsh environments, high reliability, and so on. The signals generated by an ME are always disturbed by noises; therefore, these signals are not ideal. The challenge is to achieve the highest resolution and to get the maximum operating speed as well as to use the most cost-effective hardware. To solve this problem, this paper humbly proposes an effective approach, which contains two parts: The main part is “compensating the noisy signals of MEs” by applying a new proposed method called the advanced adaptive digital phase-locked loop (AADPLL), while the second is a pulse interpolator which generates high-resolution quadrature pulses. The AADPLL algorithm provides a robust filtering characteristic to eliminate the noises and improve the accuracy of the ME's input signals. It also takes advantage of tracking high-speed input signals without time lag, unlike the traditional filters. Additionally, the computation burden is significantly reduced in this algorithm to allow it to be easily implemented in a low-cost processor. The pulse interpolator is based on an existing idea that extracts high-order sinusoids from the original ME signals. However, a new scheme is presented to achieve higher resolution per period with smaller noises affecting the output pulses. Both parts are mainly implemented in a unique hardware platform using a low-cost digital signal processor, such as the TMS320F2812, combined with a small-size field-programmable gate array. This method has already been applied to control a linear motor without using an expensive optical linear encoder. Practical results are provided to demonstrate the effectiveness of the proposed method.
Managerial ability and earnings management

The study aims to verify the relationship between the managerial ability of managers and the level of earnings management by accruals (AEM) and by real activities (REM) in Brazilian companies. Data from 2013 to 2018, corresponding to 228 non-financial companies, were analyzed through regression TOBIT. The results support a positive relationship between managerial ability and earnings management practices by accruals and real activities. Specifically, more skilled managers are associated with earnings management, which reduces the quality of accounting information. The findings support the premise that more skilled managers manage financial reporting to camouflage compensation, privileges, and excessive investment in risky projects. Furthermore, it sheds light on how individual idiosyncratic differences can affect accounting reports. persistence (Demerjian et al., 2013), and earnings smoothing (Demerjian et al., 2017; Baik et al., 2019), and REM (Huang & Sun, 2017). This study extends this literature, demonstrating that managerial ability can affect REM and AEM practices. The results of this study are in agreement with those found by Demerjian et al. (2017), but they differ from what was observed by Demerjian et al. (2013).