Miracle-dz/UniFAR-FTUs huggingface dataset:
Successful Application of Adaptive Emotion Regulation Skills Predicts the Subsequent Reduction of Depressive Symptom Severity but neither the Reduction of Anxiety nor the Reduction of General Distress during the Treatment of Major Depressive Disorder

Objective Deficits in general emotion regulation (ER) skills have been linked to symptoms of depression and are thus considered a promising target in the treatment of Major depressive disorder (MDD). However, at this point, the extent to which such skills are relevant for coping with depression and whether they should instead be considered a transdiagnostic factor remain unclear. Therefore, the present study aimed to investigate whether successful ER skills application is associated with changes in depressive symptom severity (DSS), anxiety symptom severity (ASS), and general distress severity (GDS) over the course of treatment for MDD. Methods Successful ER skills application, DSS, ASS, and GDS were assessed four times during the first three weeks of treatment in 175 inpatients who met the criteria for MDD. We computed Pearson correlations to test whether successful ER skills application and the three indicators of psychopathology are cross-sectionally associated. We then performed latent growth curve modelling to test whether changes in successful ER skills application are negatively associated with a reduction of DSS, ASS, or GDS. Finally, we utilized latent change score models to examine whether successful ER skills application predicts subsequent reduction of DSS, ASS, or GDS. Results Successful ER skills application was cross-sectionally associated with lower levels of DSS, ASS, and GDS at all points of assessment. An increase in successful skills application during treatment was associated with a decrease in DSS and GDS but not ASS. Finally, successful ER skills application predicted changes in subsequent DSS but neither changes in ASS nor changes in GDS. Conclusions Although general ER skills might be relevant for a broad range of psychopathological symptoms, they might be particularly important for the maintenance and treatment of depressive symptoms.
Physical symptoms and working performance in female breast cancer survivors: a systematic review

Abstract Purpose: The aim of this systematic review was to summarize and systematize the information about physical symptoms and its relation with work activity on female Breast Cancer Survivors (BCS). Methods: A systematic search was performed on the databases MEDLINE/PubMed (via National Library of Medicine), SCOPUS (Elsevier), Web of Science (Thomson Reuters Scientific) and CINAHL with full text (EBSCO), including papers about physical impairments experienced by female workers who have had breast cancer. Results: The search retrieved 238 studies, and another 5 were identified in the articles’ references, totaling 243 papers. After removing duplicates and applying the inclusion criteria and a full text reading, 13 articles were included for qualitative analysis. Concerning physical limitations, most complaints were related to the elevation of upper limbs, carrying heavy objects, driving and holding manual movements. The most referred symptoms were breast/arm pain, fatigue, lymphedema, reduced range of motion and weakness in the upper limbs, scar tissue adherence in the breast/axilla and paresthesia in the arm/breast. Conclusion: These symptoms and physical limitations led to the difficulty or impossibility of performing work tasks, which also diminished work productivity, as well as the increase in time to return to work. The present results suggest higher unemployment rates and the need for modifying work conditions. Implication for Rehabilitation Health professionals should include risk assessment at daily routine to identify possible sources of physical impairments for upper limbs. Provide the support and orientations according to personal and job characteristics of the patient. Focus the aims of treatment over upper limbs impairments, reducing the prevalence and the gravity of symptoms.
Additive manufacturing and postprocessing of Ti-6Al-4V for superior mechanical properties

The capabilities of metal additive manufacturing (AM) are evolving rapidly thanks to both increasing industry demand and improved scientific understanding of the process. This article provides an overview of AM of the Ti-6Al-4V alloy, which has essentially been used as a yardstick to gauge the capability of each metal AM process developed to date. It begins by summarizing the metal AM processes existing today. This is followed by a discussion of the macro- and microstructural characteristics, defects, and tensile and fatigue properties of AM Ti-6Al-4V by selective laser melting, laser metal deposition (both powder and wire), and selective electron-beam melting compared to non-AM Ti-6Al-4V. The tensile and fatigue properties of as-built AM Ti-6Al-4V (with machined or polished surfaces) can be made comparable, or even superior, to those of Ti-6Al-4V in the most commonly used mill-annealed condition. However, these properties can exhibit a large degree of scatter and are often anisotropic, affected by AM build orientations. Post-AM surface treatments or both the post-AM surface and heat treatments are necessary to ensure the minimum required properties and performance consistency. Future directions to further unlock the potential of AM of Ti-6Al-4V for superior and consistent mechanical properties are also discussed.
Incomplete Incorporation of Tandem Subunits in Recombinant Neuronal Nicotinic Receptors

Tandem constructs are increasingly being used to restrict the composition of recombinant multimeric channels. It is therefore important to assess not only whether such approaches give functional channels, but also whether such channels completely incorporate the subunit tandems. We have addressed this question for neuronal nicotinic acetylcholine receptors, using a channel mutation as a reporter for subunit incorporation. We prepared tandem constructs of nicotinic receptors by linking α (α2–α4, α6) and β (β2, β4) subunits by a short linker of eight glutamine residues. Robust functional expression in oocytes was observed for several tandems (β4_α2, β4_α3, β4_α4, and β2_α4) when coexpressed with the corresponding β monomer subunit. All tandems expressed when injected alone, except for β4_α3, which produced functional channels only together with β4 monomer and was chosen for further characterization. These channels produced from β4_α3 tandem constructs plus β4 monomer were identical with receptors expressed from monomer α3 and β4 constructs in acetylcholine sensitivity and in the number of α and β subunits incorporated in the channel gate. However, separately mutating the β subunit in either the monomer or the tandem revealed that tandem-expressed channels are heterogeneous. Only a proportion of these channels contained as expected two copies of β subunits from the tandem and one from the β monomer construct, whereas the rest incorporated two or three β monomers. Such inaccuracies in concatameric receptor assembly would not have been apparent with a standard functional characterization of the receptor. Extensive validation is needed for tandem-expressed receptors in the nicotinic superfamily.
Biotic vs. Abiotic Control of Decomposition: A Comparison of the Effects of Simulated Extinctions and Changes in Temperature

The loss of species is known to have significant effects on ecosystem functioning, but only recently has it been recognized that species loss might rival the effects of other forms of environmental change on ecosystem processes. There is a need for experimental studies that explicitly manipulate species richness and environmental factors concurrently to determine their relative impacts on key ecosystem processes such as plant litter decomposition. It is crucial to understand what factors affect the rate of plant litter decomposition and the relative magnitude of such effects because the rate at which plant litter is lost and transformed to other forms of organic and inorganic carbon determines the capacity for carbon storage in ecosystems and the rate at which greenhouse gasses such as carbon dioxide are outgassed. Here we compared how an increase in water temperature of 5°C and loss of detritivorous invertebrate and plant litter species affect decomposition rates in a laboratory experiment simulating stream conditions. Like some prior studies, we found that species identity, rather than species richness per se, is a key driver of decomposition, but additionally we showed that the loss of particular species can equal or exceed temperature change in its impact on decomposition. Our results indicate that the loss of particular species can be as important a driver of decomposition as substantial temperature change, but also that predicting the relative consequences of species loss and other forms of environmental change on decomposition requires knowledge of assemblages and their constituent species' ecology and ecophysiology.
Biogenesis of the Vaccinia Virus Membrane: Genetic and Ultrastructural Analysis of the Contributions of the A14 and A17 Proteins

ABSTRACT Vaccinia virus membrane biogenesis requires the A14 and A17 proteins. We show here that both proteins can associate with membranes co- but not posttranslationally, and we perform a structure function analysis of A14 and A17 using inducible recombinants. In the absence of A14, electron-dense virosomes and distinct clusters of small vesicles accumulate; in the absence of A17, small vesicles form a corona around the virosomes. When the proteins are induced at 12 h postinfection (hpi), crescents appear at the periphery of the electron-dense virosomes, with the accumulated vesicles likely contributing to their formation. A variety of mutant alleles of A14 and A17 were tested for their ability to support virion assembly. For A14, biologically important motifs within the N-terminal or central loop region affected crescent maturation and the immature virion (IV)→mature virion (MV) transition. For A17, truncation or mutation of the N terminus of A17 engendered a phenotype consistent with the N terminus of A17 recruiting the D13 scaffold protein to nascent membranes. When N-terminal processing was abrogated, virions attempted to undergo the IV-to-MV transition without removing the D13 scaffold and were therefore noninfectious and structurally aberrant. Finally, we show that A17 is phosphorylated exclusively within the C-terminal tail and that this region is a direct substrate of the viral F10 kinase. In vivo, the biological competency of A17 was reduced by mutations that prevented its serine-threonine phosphorylation and restored by phosphomimetic substitutions. Precleavage of the C terminus or abrogation of its phosphorylation diminished the IV→MV maturation; a block to cleavage spared virion maturation but compromised the yield of infectious virus.
Eliminating the Effect of Fog Attenuation on FSO Link by Multiple TX/RX System with Travelling Wave Semiconductor Optical Amplifier

Free Space Optics (FSO) Technology is the advanced wireless communication system that provides higher bandwidth with a higher data rate transmission over higher link range. The major challenge on free space optics technology is atmospheric attenuation that hampers data transmission of the system by increasing bit error rate. Fog is the atmospheric condition which causes highest amount of signal attenuation. Travelling wave semiconductor optical amplifier is a functional device that can eliminate the effect of fog on free space optical communication link. Multiple TX/RX system architecture with the existence of this efficient device is capable of improving the performance under fog atmospheric effect by ensuring higher received power and lower bit error rate. Our paper implements the architecture of multiple TX/RX system with travelling wave semiconductor optical amplifier under fog atmospheric condition using simulation tool Optisystem 7. The effort in this paper is to overcome the effect of fog attenuation on free space optics link by the evaluation of performance through simulated results of the implemented model in terms of Q factor, bit error rate, received power and eye height. Keywords—FSO; Fog; Travelling wave semiconductor optical amplifier; Multiple TX/RX; Eye diagram; Bit error Rate.
Feasibility and Validation of 4-D Pulse Wave Imaging in Phantoms and In Vivo

Pulse wave imaging (PWI) is a noninvasive technique for tracking the propagation of the pulse wave along the arterial wall. The 3-D ultrasound imaging would aid in objectively estimating the pulse wave velocity (PWV) vector. This paper aims to introduce a novel PWV estimation method along the propagation direction, validate it in phantoms, and test its feasibility <italic>in vivo</italic>. A silicone vessel phantom consisting of a stiff and a soft segment along the longitudinal axis and a silicone vessel with a plaque were constructed. A 2-D array with a center frequency of 2.5 MHz was used. Propagation was successfully visualized in 3-D in each phantom and <italic>in vivo</italic> in six healthy subjects. In three of the healthy subjects, results were compared against conventional PWI using a linear array. PWVs were estimated in the stiff (3.42 ± 0.23 m <inline-formula> <tex-math notation="LaTeX">$\cdot ~\text{s}^{-1}$ </tex-math></inline-formula>) and soft (2.41 ± 0.07 m <inline-formula> <tex-math notation="LaTeX">$\cdot ~\text{s}^{-1}$ </tex-math></inline-formula>) phantom segments. Good agreement was found with the corresponding static testing values (stiff: 3.41 m <inline-formula> <tex-math notation="LaTeX">$\cdot ~\text{s}^{-1}$ </tex-math></inline-formula> and soft: 2.48 m <inline-formula> <tex-math notation="LaTeX">$\cdot ~\text{s}^{-1}$ </tex-math></inline-formula>). PWI-derived vessel compliance values were validated with dynamic testing. Comprehensive views of pulse propagation in the plaque phantom were generated and compared against conventional PWI acquisitions. Good agreement was found <italic>in vivo</italic> between the results of 4-D PWI (4.80 ± 1.32 m <inline-formula> <tex-math notation="LaTeX">$\cdot ~\text{s}^{-1}$ </tex-math></inline-formula>) and conventional PWI (4.28±1.20 m <inline-formula> <tex-math notation="LaTeX">$\cdot ~\text{s}^{-1}$ </tex-math></inline-formula>) (<inline-formula> <tex-math notation="LaTeX">$n=3$ </tex-math></inline-formula>). PWVs derived for all of the healthy subjects (<inline-formula> <tex-math notation="LaTeX">$n = 6$ </tex-math></inline-formula>) were within the physiological range. Thus, the 4-D PWI was successfully validated in phantoms and used to image the pulse wave propagation in normal human subjects <italic>in vivo</italic>.
Effect of Al alloying on the Hall-Petch strengthening and AE in compressed Mg-Li-Al alloys before and after HPT processing

The paper deals with the effect of Al content in two-phase Mg-10Li-xAl (x = 0, 1, 5) alloys on their straining behaviour before and after severe plastic deformation by high pressure torsion. Both coarse-grained and ultrafine-grained alloys were compression strained in channel-die device with simultaneous acoustic emission monitoring. It has been observed that Hall-Petch strengthening in ultra-fine grained alloys superposes with the strengthening resulting from Al alloying (solution hardening, particulate strengthening). Superposition of these strengthening mechanisms becomes stronger with increase in Al content, presumably due to enhanced grain refining effect. K e y w o r d s: Mg-Li-Al alloys, grain refinement, high pressure torsion, acoustic emission, Hall-Petch strengthening
Explaining Intention to Use an Information Technology Innovation: an empirical comparison of the perceived characteristics of innovating and technology acceptance models

This study examines the issue of technology acceptance in a multi-campus secondary college in Sydney, Australia. Seventy-five teachers across two campuses were surveyed as to their perceptions regarding technology acceptance. Regression analysis was used to compare the explanatory power of the perceived characteristics of innovating model (PCIM), and the technology acceptance model (TAM). Both models explained a substantial amount of variation in technology acceptance. However, our findings suggest that it is preferable to use the PCIM, rather than the TAM, to explain intention to use an information technology innovation. Implications for both future research and practice are discussed.
Root responses to nutrients and soil biota: drivers of species coexistence and ecosystem productivity

1. Although a major part of plant biomass is underground, we know little about the contribution of different species to root biomass in multispecies communities. We summarize studies on root distributions and plant responses to species‐specific soil biota and formulate three hypotheses to explain how root responses may drive species coexistence and ecosystem productivity.
Biomaterial-based Strategies to Build Vascularized Modular Tissue Engineered Constructs

Survival of engineered tissues in vivo requires the presence of an internal vascular network and immediate connection to the host vasculature. Modular tissue engineering approaches the vascularization ‘design’ requirement through fabrication of submillimeter-sized collagen microtissues (‘modules’) with endothelial cells (EC) seeded on the surface of the modules and functional or vascular support cells inside the modules. Several modules are then packed together to build a larger tissue. In this work, we explored biomaterial-based strategies to build vascularized modular tissue engineered constructs. A photocrosslinkable poloxamine-polylysine acrylate biomaterial was first synthesized to improve the mechanical limitations of collagen modules under flow, while still supporting EC attachment. An extracellular matrix (ECM)-based strategy was then explored to enhance the vascularization of the modules in vivo. Manipulation of the ECM was accomplished through lentiviral transduction of EC to overexpress Developmental endothelial locus-1 (Del-1), a pro-angiogenic ECM molecule. Supporting the hypothesis that Del-1 overexpression ‘tilts’ the balance in EC from a quiescent to a pro-
Celiac Disease Diagnosis Without Biopsy: Is a 10× ULN Antitransglutaminase Result Suitable for a Chemiluminescence Method?

Objectives: 2012 European Society of Pediatric Gastroenterology, Hepatology and Nutrition guidelines allow to establish a celiac disease diagnosis without duodenal biopsy in symptomatic pediatric patients with antitissue transglutaminase (anti-tTG) titers >10 times the upper limit of normal. For some years now, new chemiluminescence immunoassays have been made available: it is important to establish the clinical performance of anti-tTG and to determine the cut-off best suited to predict Marsh ≥2 to avoid gastrointestinal endoscopy not only in children, but also in the adult population. Methods: A total of 2565 patients performed duodenal biopsy from July 2012 to September 2016; we selected all the patients who had undergone QUANTA Flash anti-tTG immunoglobulin A (IgA) within −3 months of duodenal biopsy and before the start of gluten-free diet. A total of 827 patients fulfilled the criteria for selection. Results: Using a cut-off of 20 chemiluminescent unit (CU; area under the curve: 0.995), sensitivity, specificity, positive, and negative predictive value were 98.2%, 98.4%, 97.9%, and 98.6%, respectively. For the correlation with Marsh ≥2, in the pediatric population, positive predictive values (PPV) were 92.1%, 99%, and 100% at 200 CU (10×), 560 CU (28×), and 1000 CU (50×), respectively. In the adult population PPV was 94.2%, 98.2%, and 100% at 200 CU (10×), 350 CU (15×), and 400 CU (20×). Conclusions: Sensitivity, specificity, positive, and negative predictive value of QUANTA Flash h-tTG IgA were excellent. The cut-off providing an optimized PPV for histological lesions compatible for celiac disease (Marsh ≥2) for the QUANTA Flash h-tTG IgA is 350 CU (15×) in adult and 560 CU (28×) in children.
Resilience of mycorrhizal fungi on defoliated and fertilized hybrid poplars

We examined the effects of fertilization and gypsy moth defoliation of hybrid poplar (Populus ×canadensis Moench 'Eugenei') on ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) fungal colonization, ECM richness, and ECM composition in the summers of 1997 and 1998. The factorial experiment included two levels of defoliation (defoliated and control) and fertilization (100 kg N·ha -1 and control). Gypsy moth (Lymantria dispar L.) populations were manipulated to obtain defoliation in the summer of 1996, 1997, and 1998; fertilization subplots were fertilized with NH4NO3 (100 kg N·ha -1 ) in the spring of these years. There were no significant effects of defoliation on ECM or AM colonization in either year; there was a significant (p ≤ 0.05) decline in AM colonization in fertilized plots in 1997 and a significant interaction between defoliation and fertilization effects on ECM colonization in 1997. In the nondefoliated plots, ECM fungal colonization increased, whereas AM colonization decreased because of fertilization. In the defoliated plots, neither ECM nor AM colonization was affected by fertilization. ECM community composition and richness were unchanged by any treatment. The small and transient effects of defoliation and fertilization on poplar AMs and ECMs demonstrate the tolerance of these early-successional trees to defoliation and their ability to rapidly use high levels of available nitrogen.
Gain-of-function mutations in granulocyte colony–stimulating factor receptor (CSF3R) reveal distinct mechanisms of CSF3R activation

Granulocyte colony–stimulating factor (G-CSF or CSF3) and its receptor CSF3R regulate granulopoiesis, neutrophil function, and hematopoietic stem cell mobilization. Recent studies have uncovered an oncogenic role of mutations in the CSF3R gene in many hematologic malignancies. To find additional CSF3R mutations that give rise to cell transformation, we performed a cellular transformation assay in which murine interleukin 3 (IL-3)–dependent Ba/F3 cells were transduced with WT CSF3R plasmid and screened for spontaneous growth in the absence of IL-3. Any outgrowth clones were sequenced to identify CSF3R mutations with transformation capacity. We identified several novel mutations and determined that they transform cells via four distinct mechanisms: 1) cysteine- and disulfide bond–mediated dimerization (S581C); 2) polar, noncharged amino acid substitution at the transmembrane helix dimer interface at residue Thr-640; 3) increased internalization by a Glu-524 substitution that mimics a low G-CSF dose; and 4) hydrophobic amino acid substitutions in the membrane-proximal residues Thr-612, Thr-615, and Thr-618. Furthermore, the change in signaling activation was related to an altered CSF3R localization. We also found that CSF3R-induced STAT3 and ERK activations require CSF3R internalization, whereas STAT5 activation occurred at the cell surface. Cumulatively, we have expanded the regions of the CSF3R extracellular and transmembrane domains in which missense mutations exhibit leukemogenic capacity and have further elucidated the mechanistic underpinnings that underlie altered CSF3R expression, dimerization, and signaling activation.