Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact of Antibiotic Treatment on Bacterial Resistance Rates from Patients with Hospital-Acquired Infection

Abstract We assessed the impact of antibiotic administration prior to sample collection on the bacterial resistance rates from patients with nosocomial infection. Every individual susceptibility report was assessed in real time at the bedside of the patient by a team composed of infectious diseases and internal medicine specialists as well as clinical microbiologists for clinical significance and appropriateness of the specimen. The report also stated the kind, source and origin of the infection, history of administration of any antibiotic during the last month prior to sample collection. To evaluate the impact of previous antibiotic administration, resistance rates were calculated separately among the group of patients with and without history of antibiotic treatment. A crude univariate analysis was performed to assess the significance of the differences between groups for every species-antibiotic pair. Patients who had received ciprofloxacin showed significantly higher rates of Escherichia coli resistant to ciprofloxacin, broad-spectrum cephalosporins and gentamicin. A higher rate of methicillin-resistant Staphylococcus aureus was observed in patients who were given gentamicin. A stratified analysis showed that the previous antibiotic administration continued to be a risk factor for increased resistance rates regardless of the hospital ward or the source of the infection. This study demonstrates the influence of previous antibiotic administration on bacterial resistance rates although this fact is barely taken into account by the laboratory when constructing the cumulative susceptibility data. Real time clinical validation of the individual susceptibility reports, performed by a multidisciplinary team prior to the data entering, might be a suitable approach to get more reliable susceptibility rates to guide the rational selection of antimicrobial empirical therapy in patients with hospital-acquired infections who have been given antimicrobial treatment prior to specimen collection.
The photographic representation of female athletes in the British print media during the London 2012 Olympic Games

The London 2012 Olympic Games were widely reported as the ‘Women's Games’ but was this reflected in the photographic representation in British print media? This study focused upon photographic representation of athletes in the British print media during the London 2012 Olympic Games. The original dimension of this investigation is the triangulation of gender with location, position and camera angle of photos. From a feminist perspective, content analysis was used to compare the amount and prominence of the coverage devoted to female and male athletes and photographs during the Games findings indicate that media coverage of female athletes continues to lag behind that of male athletes in quantity of photos. However, there are signs of increased gender equality in the location, page prominence and camera angle of photos of sportswomen compared to previous studies on the media representation of female athletes at Olympic Games.
The Chemokine RANTES Is a Crucial Mediator of the Progression from Acute to Chronic Colitis in the Rat1

Chemokines have well characterized proinflammatory actions, including the ability to induce extravasation of leukocytes that participate in chronic inflammation. In this study, we evaluated the role of a C-C chemokine, RANTES, in the chronic phase of a rat model of colitis. Colitis was induced by intracolonic administration of trinitrobenzene sulfonic acid. At various timepoints thereafter (2 h to 14 days), colonic tissue levels of several chemokines were measured. Unlike the expression of monocyte chemoattractant protein-1, macrophage inflammatory protein-2, and cytokine-induced neutrophil chemoattractant, the expression of RANTES was significantly elevated during the chronic phase of colitis (≥7 days after induction). Colonic RANTES mRNA expression was also significantly elevated during the chronic phase of colitis. The numbers of macrophages and monocytes in the colonic mucosa increased substantially during the chronic phase, as did expression of two of the receptors (CCR1 and CCR5) to which RANTES is known to bind. Administration on days 7 through 14 after trinitrobenzene sulfonic acid administration of a CCR1/CCR5 receptor antagonist, Met-RANTES, resulted in a significant reduction of both macroscopic and microscopic colonic damage, as well as reducing the recruitment into the colon of monocytes, mast cells, and neutrophils. In some rats, treatment with Met-RANTES resulted in a near-complete resolution of colonic damage and inflammation. These results suggest a crucial role of RANTES in the progression from acute to chronic inflammation in a rat model of colitis.
Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures

Background: Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. TPC and TFC are the principle activity constituents present in the plant. The effects of extraction temperature on TPC, TFC and free radical-scavenging capacity of Gynura divaricata leaf extracts are worth to study. Materials and Methods: Folin–Ciocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. The antioxidant and free radical-scavenging activity were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and phosphomolybdenum methods. Results: TPC and TFC were significantly elevated with increasing extraction temperature (from 40°C to 100°C). However, TPC and TFC were not significantly different (P > 0.05) at the extraction temperatures 90°C and 100°C. Also, the extracts obtained at a higher temperature exhibited a significant free radical-scavenging activity compared with extraction at lower temperatures (P < 0.05). The TPCs (13.95-36.68 mg gallic acid equivalent/g dry material) were highly correlated with DPPH (R2 = 0.9229), ABTS (R2 = 0.9951) free radical-scavenging capacity, and total antioxidant activity (R2 = 0.9872) evaluated by phosphomolybdenum method. Conclusion: The TPC and TFC of G. divaricata leaf was significantly influenced by the extraction temperatures, which were the main antioxidant constituents present in the G. divaricata plant.
Acetaminophen Self-Poisoning: Suicidal and Accidental

Objective: Acetaminophen is an over-the-counter drug in Iran. Intentional and accidental poisoning with this drug is one of the most frequent causes of admission to our center. We studied the outcome of poisoning with this drug in our hospital. Methods: Over a two-year period from January 2005 to January 2007, 85 patients who were admitted to Sina Hospital for acute acetaminophen poisoning, were followed up. Identification and outcome of patients were according to a physical examination, medical history, lab data and duration of hospital stay. Results: Acute acetaminophen poisoning occurred in both genders and all age groups. Approximately 64 percent of patients were female. Children had minimal involvement and were usually accidental poisoning type (98%). In adults, admissions were more likely to be due to suicide attempts rather than accidental poisoning. The majority of cases were in hepatotoxic dose, but clinical courses were mild. Patients, who had acutely ingested more than 150 mg/kg or predicted to be hepatotoxic due to impaired liver function testes, had a longer hospital stay but in spite of this, we did not have any mortality. Conclusion: Overdose with acetaminophen in adults was often indications of suicidal behavior, and in children was usually of accidental type. The outcome was generally good in spite of common acetaminophen poisoning.
Viability of human septal cartilage after 1.45 µm diode laser irradiation

Chondrocyte viability following laser irradiation and reshaping has not been established for human nasal septal cartilage. Knowledge of the relationship between thermal injury and laser dosimetry is needed in order to optimize septal laser cartilage reshaping. The objective of this study was to determine the depth and width of thermal injury in human septal cartilage following laser irradiation.
On the Frequency of Use of Tenses in English and German Children's Spontaneous Speech.

SZAGUN, GISELA. On the Frequency of Use of Tenses in English and German Children's Spontaneous Speech. CHmLD DEVELOPMENT, 1978, 49, 898-901. This cross-cultural study examined the frequency of use of tenses by English and German children between 2-6 and 4-6. Developmental changes in frequency of use of tenses were observed: frequency of use of present tenses decreased significantly with age, at the same rate and level in both languages, and frequency of past and future tenses increased correspondingly, with past tenses increasing more rapidly. Children's frequencies did not correlate with adult frequencies of use of tenses. The children's frequencies of use of present, past, and future tenses were interpreted as indicative of their cognitive organization of temporal experience.
Seismic Reflection Views of the 1994 Northridge Earthquake Hypocentral Region Using Aftershock Data and Imaging Techniques

Earthquake hazard assessment in the San Fernando Valley, California, requires knowledge of the existence and geometry of blind thrust faults. We show that crustal-scale reflection imaging for this region is feasible using aftershock data from a regional network of short-period vertical seismometers. We image regional fault structures based on the reflectivity structure beneath the hypocentral region of the 1994 Northridge earthquake. Our work combines simple data editing and imaging techniques borrowed from exploration seismology. Our results show the potential of migrated depth sections in defining major reflectors and strongly suggest that the use of existing aftershock data offers a cost effective and straightforward alternative for an integration of seismic reflection data in this and similar areas.
Simulating the Impact of Improved Cardiovascular Risk Interventions on Clinical and Economic Outcomes in Russia

Objectives Russia faces a high burden of cardiovascular disease. Prevalence of all cardiovascular risk factors, especially hypertension, is high. Elevated blood pressure is generally poorly controlled and medication usage is suboptimal. With a disease-model simulation, we forecast how various treatment programs aimed at increasing blood pressure control would affect cardiovascular outcomes. In addition, we investigated what additional benefit adding lipid control and smoking cessation to blood pressure control would generate in terms of reduced cardiovascular events. Finally, we estimated the direct health care costs saved by treating fewer cardiovascular events. Methods The Archimedes Model, a detailed computer model of human physiology, disease progression, and health care delivery was adapted to the Russian setting. Intervention scenarios of achieving systolic blood pressure control rates (defined as systolic blood pressure <140 mmHg) of 40% and 60% were simulated by modifying adherence rates of an antihypertensive medication combination and compared with current care (23.9% blood pressure control rate). Outcomes of major adverse cardiovascular events; cerebrovascular event (stroke), myocardial infarction, and cardiovascular death over a 10-year time horizon were reported. Direct health care costs of strokes and myocardial infarctions were derived from official Russian statistics and tariff lists. Results To achieve systolic blood pressure control rates of 40% and 60%, adherence rates to the antihypertensive treatment program were 29.4% and 65.9%. Cardiovascular death relative risk reductions were 13.2%, and 29.6%, respectively. For the current estimated 43,855,000-person Russian hypertensive population, each control-rate scenario resulted in an absolute reduction of 1.0 million and 2.4 million cardiovascular deaths, and a reduction of 1.2 million and 2.7 million stroke/myocardial infarction diagnoses, respectively. Averted direct costs from current care levels ($7.6 billion [in United States dollars]) were $1.1 billion and $2.6 billion, respectively.
Retnla Overexpression Attenuates Allergic Inflammation of the Airway

Resistin-like molecule alpha (Retnla), also known as ‘Found in inflammatory zone 1’, is a secreted protein that has been found in bronchoalveolar lavage (BAL) fluid of ovalbumin (OVA)-induced asthmatic mice and plays a role as a regulator of T helper (Th)2-driven inflammation. However, the role of Retnla in the progress of Th2-driven airway inflammation is not yet clear. To better understand the function of Retnla in Th2-driven airway inflammation, we generated Retnla-overexpressing (Retnla-Tg) mice. Retnla-Tg mice showed increased expression of Retnla protein in BAL fluid and airway epithelial cells. Retnla overexpression itself did not induce any alteration in lung histology or lung function compared to non-Tg controls. However, OVA-sensitized/challenged Retnla-Tg mice had decreased numbers of cells in BAL and inflammatory cells accumulating in the lung. They also showed a reduction in mucus production in the airway epithelium, concomitant with a decreased Muc5ac level. These results were accompanied by reduced levels of Th2 cytokines, including interleukin (IL)-4, IL-5, and IL-13, with no effect on levels of OVA-specific immunoglobulin isotypes. Furthermore, phosphorylation of ERK was markedly reduced in the lungs of OVA-challenged Retnla-Tg mice. Taken together, these results indicates that Retnla protects against Th2-mediated inflammation in an experimental mouse model of asthma, suggesting that therapeutic approaches to enhance the production of Retnla or Retnla-like molecules could be valuable for preventing allergic lung inflammation.
Instability of CAG and CTG trinucleotide repeats in Saccharomyces cerevisiae

A quantitative genetic assay was developed to monitor alterations in tract lengths of trinucleotide repeat sequences in Saccharomyces cerevisiae. Insertion of (CAG)50 or (CTG)50 repeats into a promoter that drives expression of the reporter gene ADE8 results in loss of expression and white colony color. Contractions within the trinucleotide sequences to repeat lengths of 8 to 38 restore functional expression of the reporter, leading to red colony color. Reporter constructs including (CAG)50 or (CTG)50 repeat sequences were integrated into the yeast genome, and the rate of red colony formation was measured. Both orientations yielded high rates of instability (4 x 10(-4) to 18 x 10(-4) per cell generation). Instability depended on repeat sequences, as a control harboring a randomized (C,A,G)50 sequence was at least 100-fold more stable. PCR analysis of the trinucleotide repeat region indicated an excellent correlation between change in color phenotype and reduction in length of the repeat tracts. No preferential product sizes were observed. Strains containing disruptions of the mismatch repair gene MSH2, MSH3, or PMS1 or the recombination gene RAD52 showed little or no difference in rates of instability or distributions of products, suggesting that neither mismatch repair nor recombination plays an important role in large contractions of trinucleotide repeats in yeast.
COGNITIVE RESTRUCTURING WITHIN RELIVING: A TREATMENT FOR PERITRAUMATIC EMOTIONAL “HOTSPOTS” IN POSTTRAUMATIC STRESS DISORDER

This paper describes a distinct clinical approach to the treatment of Posttraumatic Stress Disorder (PTSD). It is theoretically guided by recent cognitive models of PTSD and explicitly combines cognitive therapy techniques within exposure/reliving procedures. A clinically pertinent distinction is made between the cognitions and emotions experienced at the time of the trauma and, subsequently, in flashback experiences, and secondary negative appraisals. The term peritraumatic emotional “hotspot” is used to describe moments of peak distress during trauma. It is argued that a focus on cognitively restructuring these peritraumatic emotional hotspots within reliving can significantly improve the effectiveness of the treatment of PTSD and help explain some treatment failures with traditional prolonged exposure. An approach to the identification and treatment of these hotspots is detailed for a range of cognitions and emotions not limited to fear.
Modeling pixel means and covariances using factorized third-order boltzmann machines

Learning a generative model of natural images is a useful way of extracting features that capture interesting regularities. Previous work on learning such models has focused on methods in which the latent features are used to determine the mean and variance of each pixel independently, or on methods in which the hidden units determine the covariance matrix of a zero-mean Gaussian distribution. In this work, we propose a probabilistic model that combines these two approaches into a single framework. We represent each image using one set of binary latent features that model the image-specific covariance and a separate set that model the mean. We show that this approach provides a probabilistic framework for the widely used simple-cell complex-cell architecture, it produces very realistic samples of natural images and it extracts features that yield state-of-the-art recognition accuracy on the challenging CIFAR 10 dataset.
Top-Down Mass Analysis of Protein Tyrosine Nitration: Comparison of Electron Capture Dissociation with “Slow-Heating” Tandem Mass Spectrometry Methods

Tyrosine nitration in proteins is an important post-translational modification (PTM) linked to various pathological conditions. When multiple potential sites of nitration exist, tandem mass spectrometry (MS/MS) methods provide unique tools to locate the nitro-tyrosine(s) precisely. Electron capture dissociation (ECD) is a powerful MS/MS method, different in its mechanisms to the “slow-heating” threshold fragmentation methods, such as collision-induced dissociation (CID) and infrared multiphoton dissociation (IRMPD). Generally, ECD provides more homogeneous cleavage of the protein backbone and preserves labile PTMs. However recent studies in our laboratory demonstrated that ECD of doubly charged nitrated peptides is inhibited by the large electron affinity of the nitro group, while CID efficiency remains unaffected by nitration. Here, we have investigated the efficiency of ECD versus CID and IRMPD for top-down MS/MS analysis of multiply charged intact nitrated protein ions of myoglobin, lysozyme, and cytochrome c in a commercial Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. CID and IRMPD produced more cleavages in the vicinity of the sites of nitration than ECD. However the total number of ECD fragments was greater than those from CID or IRMPD, and many ECD fragments contained the site(s) of nitration. We conclude that ECD can be used in the top-down analysis of nitrated proteins, but precise localization of the sites of nitration may require either of the “slow-heating” methods.
Attributions of Blame and Credibility in a Hypothetical Child Sexual Abuse Case: Roles of victim disability, victim resistance and respondent gender

This study examines the effects victim disability (physical vs. intellectual vs. none), victim resistance (physical vs. verbal vs. none) and respondent gender (male vs. female) have on attributions of blame and credibility in a hypothetical case of child sexual abuse. Three hundred and thirty‐five respondents read a fictional police statement regarding the sexual assault of a 12‐year‐old girl by a 23‐year‐old man before completing 28 attribution items. Principal axis factoring revealed six reliable factors. Subsequent multivariate analysis of covariance—controlling for respondents’ general attitude towards disability—revealed that males deemed the victim more culpable for her own abuse than did females. Further, perpetrators were deemed more culpable when the victim physically (vs. verbally) resisted. Finally, a significant three‐way interaction suggests victim resistance influences attributions of perpetrator blame given a victim’s disability status, at least amongst male observers. Implications and proposals for future research are discussed.
Screening Primary-Care Patients Forgoing Health Care for Economic Reasons

Background Growing social inequities have made it important for general practitioners to verify if patients can afford treatment and procedures. Incorporating social conditions into clinical decision-making allows general practitioners to address mismatches between patients' health-care needs and financial resources. Objectives Identify a screening question to, indirectly, rule out patients' social risk of forgoing health care for economic reasons, and estimate prevalence of forgoing health care and the influence of physicians' attitudes toward deprivation. Design Multicenter cross-sectional survey. Participants Forty-seven general practitioners working in the French–speaking part of Switzerland enrolled a random sample of patients attending their private practices. Main Measures Patients who had forgone health care were defined as those reporting a household member (including themselves) having forgone treatment for economic reasons during the previous 12 months, through a self-administered questionnaire. Patients were also asked about education and income levels, self-perceived social position, and deprivation levels. Key Results Overall, 2,026 patients were included in the analysis; 10.7% (CI95% 9.4–12.1) reported a member of their household to have forgone health care during the 12 previous months. The question “Did you have difficulties paying your household bills during the last 12 months” performed better in identifying patients at risk of forgoing health care than a combination of four objective measures of socio-economic status (gender, age, education level, and income) (R2 = 0.184 vs. 0.083). This question effectively ruled out that patients had forgone health care, with a negative predictive value of 96%. Furthermore, for physicians who felt powerless in the face of deprivation, we observed an increase in the odds of patients forgoing health care of 1.5 times. Conclusion General practitioners should systematically evaluate the socio-economic status of their patients. Asking patients whether they experience any difficulties in paying their bills is an effective means of identifying patients who might forgo health care.
Time Allocation in Carpenter Ants (Componotus herculeanus)

In three experiments we examined the time allocations of carpenter ants (Componotus herculeanus) that chose between different rates of brief deliveries of the odor of peppermint oil, an aversive stimulus for ants. The odor deliveries were programmed by two variable-time schedules that ran either simultaneously or successively. In both cases the relative time allocations varied systematically with respect to the relative local (number per time) rates of odor delivery. In addition, the relative time allocations were more extreme when the two schedules ran simultaneously instead of successively. Similar results were found for rats that chose between different rates of shock delivery (Deluty & Church, 1978). We suggest that one process that underlies the time allocations of both species is the elicitation of activity by the aversive stimuli. Relative differences in the time allocations of ants and rats across the two types of schedules may also reflect a species difference.