Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer

Metastasis is the ultimate cause of breast cancer related mortality. Epithelial-mesenchymal transition (EMT) is thought to play a crucial role in the metastatic potential of breast cancer. Growing evidence has implicated the SUMO E3 ligase PIAS1 in the regulation of EMT in mammary epithelial cells and breast cancer metastasis. However, the relevance of PIAS1 in human cancer and mechanisms by which PIAS1 might regulate breast cancer metastasis remain to be elucidated. Using tissue-microarray analysis (TMA), we report that the protein abundance and subcellular localization of PIAS1 correlate with disease specific overall survival of a cohort of breast cancer patients. In mechanistic studies, we find that PIAS1 acts via sumoylation of the transcriptional regulator SnoN to suppress invasive growth of MDA-MB-231 human breast cancer cell-derived organoids. Our studies thus identify the SUMO E3 ligase PIAS1 as a prognostic biomarker in breast cancer, and suggest a potential role for the PIAS1-SnoN sumoylation pathway in controlling breast cancer metastasis.
Experimental performance characterization of a meshed network for the Smart Grid

Telecommunication is a prerequisite for the Smart Grid. Several technologies are available on the market, but there is not a general agreement about which of them, or combination of them, that can enable most of the services for the distribution grid automation. The paper describes the approach of 7th Framework Program European Project INTEGRIS which relies on the hypothesis that there is not a single technology with an optimal trade-off between cost of investment and performances for all use cases and topologies of a distribution grid. It rather suggests to mesh several technologies (with focus on the Broadband Power Line, the Wi-Fi and the Fiber Optics) to cover all the segment of the distribution grid. This concept has been experimentally validated in two field demonstrators in Italy and in Spain. This paper presents the results of tests on the latency and the throughput.
Impact of a Bifenthrin-Treated Lethal Ovitrap on Aedes aegypti Oviposition and Mortality in North Queensland, Australia

Abstract Lethal ovitraps (LOs) containing an insecticide-treated ovistrip are used as a lure-and-kill device for the container-breeding dengue vector, Aedes aegypti (L.). We aimed to affirm that the pyrethroid bifenthrin could be used effectively in LOs against Ae. aegypti in north Queensland, Australia, by quantifying oviposition in and mortality caused by LOs. Small cage experiments in which individual gravid Ae. aegypti were given a choice of LOs and untreated ovitraps revealed that although LOs were less acceptable for oviposition, they provided an average 64.6% adjusted mortality. Although 92% of mosquitoes ovipositing in LOs died, 61.8% of mosquitoes that visited but did not oviposit in an LO also died, demonstrating that lethal contact occurred without egg laying. The bifenthrin content of strips (≈0.1 mg/cm2; 7 mg/strip) did not decrease significantly after 4 wk of field exposure nor did the toxic effect of the LOs. Large cage trials with groups of 10 Ae. aegypti confirmed that bifenthrin-treated LOs provided consistent control (average adjusted mortality 79.7%). Four-week field trials in north Queensland showed that although LOs were acceptable to ovipositing Ae. aegypti (mean time to first egg 10.9 d; mean eggs 47.3), insecticide-free ovitraps were oviposited in more readily (6.8 d, 199 eggs). The number of eggs laid per mosquito in laboratory LOs allowed calculation of the number of Ae. aegypti killed in field-deployed LOs; rapid estimates can be made by simply dividing the number of eggs on the strip by 2.84. Overall, the studies demonstrated that bifenthrin-treated LOs have potential for use as a lure-and-kill device against Ae. aegypti and that they should be effective in the field for at least 4 wk. Given that untreated ovitraps were more acceptable for Ae. aegypti oviposition, the removal of alternative oviposition sites before deployment of LOs in the field should maximize their effectiveness.
Is depression associated with microvascular disease in patients with type 2 diabetes?

We hypothesize that late‐life depression is a manifestation of microvascular disease in patients with type 2 diabetes. We conducted a clinic‐based cross‐sectional study, comparing retinal vascular caliber, a marker of microvascular disease, in participants with type 2 diabetes with major depression (n=34), without depression (n=27) and healthy non‐diabetic controls (n=38). Retinal vascular caliber was measured from digital retinal photographs using a validated computer‐assisted method. After adjusting for age and gender, there was a trend of increasing retinal arteriolar caliber from healthy controls (132.6 μm), to diabetic patients without depression (139.2 μm), and diabetic patients with major depression (145.3 μm, P=0.008). The trend in retinal arteriolar caliber remains significant after adjusting for duration of diabetes, but not after further adjusting for vascular risk factors. Our findings suggest that there is variation in the retinal vascular caliber between type 2 diabetic patients with and without major depression and non‐diabetic controls. This variation was largely related to poorer diabetes control and a higher frequency of vascular risk factors in diabetic patients, particularly those with depression. Studies with larger sample size may provide further insights into this association. Depression and Anxiety, 2008. Published 2007 Wiley‐Liss, Inc.
Calcium transients in single mammalian skeletal muscle fibres.

1. We studied the transient changes in myoplasmic Ca2+ concentration under current‐ and voltage‐clamp (double Vaseline‐gap technique) in cut fibres of rat extensor digitorum longus muscle using mag‐fura‐2 (furaptra) as Ca2+ indicator, at 3.6‐3.8 microns sarcomere length and 17 degrees C. Mag‐fura‐5 and fura‐2 were also used in order to characterize some aspects of the Ca2+ transients. 2. The peak [Ca2+] in response to a single action potential was 4.6 +/‐ 0.4 microM (n = 5). The time to peak of the Ca2+ transient was 4.6 +/‐ 0.42 ms, with half‐width of 8.2 +/‐ 1.5 ms, time constant of the rising phase 1.15 +/‐ 0.25 ms, time constant of the decaying phase 3.26 +/‐ 0.65 ms, and delay between action potential and Ca2+ transient 2.0 +/‐ 0.2 ms. 3. Ca2+ transients were studied under voltage‐clamp conditions at different voltages and pulse durations. The rising phase showed a complex temporal course with a fast initial increase and a second component. Both components were separated by a plateau or a brief decrease of the Ca2+ concentration. The peak Ca2+ transient was 10.5 +/‐ 1.3 microM (n = 22). 4. After interrupting the pulse, Ca2+ concentration decayed exponentially. The time constant of decay of the Ca2+ transient increased with the pulse voltage and duration, reaching a maximum value at potentials more positive than +10 mV and pulses longer than 200 ms. An analysis of the decaying phases of the Ca2+ transients suggests that only the removal process operates after fibre repolarization. 5. The rate of Ca2+ release from the sarcoplasmic reticulum was calculated using the Melzer, Ríos & Schneider model. The value of 17.2 +/‐ 3.1 micronM ms‐1 (n = 10) estimated in these calculations was intermediate between those obtained by other authors from cut frog muscles (10 microM ms‐1) and intact frog fibres (100 microM ms‐1) using antipyrylazo III (AP III) as the Ca2+ indicator.
Investigation of Growth Conditions of CdTe Thick Films on Properties and Demands for X-Ray Detector Applications

CdTe thick films were prepared by vacuum deposition on amorphous substrates using MBE technique. The growth was performed at different temperatures to investigate the development of the growth rate, surface morphology, structure and optical properties. Properties of films deposited with a single CdTe source are compared with films grown with an additional Cd source. The growth experiments are discussed with regard to demands for X-ray detector applications.
Long-term clinical and neuropsychological outcomes of West Nile virus infection.

BACKGROUND Since its introduction in 1999, West Nile virus has rapidly become the most common arboviral infection in North America. Little is known about the long-term clinical sequelae of West Nile virus infection. METHODS A total of 49 patients with laboratory-confirmed West Nile virus infection were identified through state-based surveillance. Stratification for disease severity was based on hospitalization during the infection episode. Assessment occurred a mean of 13 months after diagnosis. Medical records were reviewed, and a complete neurologic examination was performed. Standardized surveys for quality of life, functional ability, fatigue, and depression were performed for all subjects. An extensive battery of neuropsychological tests was performed to assess cognitive function. RESULTS Self-reported fatigue, memory problems, extremity weakness, word-finding difficulty, and headache were common complaints. Standardized survey data confirmed an overall sense of poor physical health, fatigue, depression, and moderate-to-severe disability in 24 (49%), 24 (49%), 12 (24%), and 4 (8%) patients, respectively. New tremor was seen or reported for 10 (20%) of the patients. Neuropsychological testing showed abnormalities of motor skills, attention, and executive functions. Univariate analysis of multiple risk factors did not identify any predictors of adverse outcomes. CONCLUSIONS Multiple somatic complaints, tremor, and abnormalities in motor skills and executive functions are common long-term problems among patients who have had West Nile virus infection. Patients with milder illness are just as likely as patients with more-severe illness to experience adverse outcomes.
Task-dependent neural representations of salient events in dynamic auditory scenes

Selecting pertinent events in the cacophony of sounds that impinge on our ears every day is regulated by the acoustic salience of sounds in the scene as well as their behavioral relevance as dictated by top-down task-dependent demands. The current study aims to explore the neural signature of both facets of attention, as well as their possible interactions in the context of auditory scenes. Using a paradigm with dynamic auditory streams with occasional salient events, we recorded neurophysiological responses of human listeners using EEG while manipulating the subjects' attentional state as well as the presence or absence of a competing auditory stream. Our results showed that salient events caused an increase in the auditory steady-state response (ASSR) irrespective of attentional state or complexity of the scene. Such increase supplemented ASSR increases due to task-driven attention. Salient events also evoked a strong N1 peak in the ERP response when listeners were attending to the target sound stream, accompanied by an MMN-like component in some cases and changes in the P1 and P300 components under all listening conditions. Overall, bottom-up attention induced by a salient change in the auditory stream appears to mostly modulate the amplitude of the steady-state response and certain event-related potentials to salient sound events; though this modulation is affected by top-down attentional processes and the prominence of these events in the auditory scene as well.
Impact of Male Circumcision on the HIV Epidemic in Papua New Guinea: A Country with Extensive Foreskin Cutting Practices

The degree to which adult medical male circumcision (MC) programs can reduce new HIV infections in a moderate HIV prevalence country like Papua New Guinea (PNG) are uncertain especially given the widespread prevalence of longitudinal foreskin cuts among adult males. We estimated the likely impact of a medical MC intervention in PNG using a mathematical model of HIV transmission. The model was age-structured and incorporated separate components for sex, rural/urban, men who have sex with men and female sex workers. Country-specific data of the prevalence of foreskin cuts, sexually transmitted infections, condom usage, and the acceptability of MC were obtained by our group through related studies. If longitudinal foreskin cutting has a protective efficacy of 20% compared to 60% for MC, then providing MC to 20% of uncut males from 2012 would require 376,000 procedures, avert 7,900 HIV infections by 2032, and require 143 MC per averted infection. Targeting uncut urban youths would achieve the most cost effective returns of 54 MC per HIV infection averted. These numbers of MC required to avert an HIV infection change little even with coverage up to 80% of men. The greater the protective efficacy of longitudinal foreskin cuts against HIV acquisition, the less impact MC interventions will have. Dependent on this efficacy, increasing condom use could have a much greater impact with a 10 percentage point increase averting 18,400 infections over this same period. MC programs could be effective in reducing HIV infections in PNG, particularly in high prevalence populations. However the overall impact is highly dependent on the protective efficacy of existing longitudinal foreskin cutting in preventing HIV.
Small Conductance Ca2+-activated K+ Channels and Calmodulin

Small conductance Ca2+-activated K+ channels (SK channels) are heteromeric complexes of pore-forming α subunits and constitutively bound calmodulin (CaM). The binding of CaM is mediated in part by the electrostatic interaction between residues Arg-464 and Lys-467 of SK2 and Glu-84 and Glu-87 of CaM. Heterologous expression of the double charge reversal in SK2, SK2 R464E/K467E (SK2:64/67), did not yield detectable surface expression or channel activity in whole cell or inside-out patch recordings. Coexpression of SK2:64/67 with wild type CaM or CaM1,2,3,4, a mutant lacking the ability to bind Ca2+, rescued surface expression. In patches from cells coexpressing SK2:64/67 and wild type CaM, currents were recorded immediately following excision into Ca2+-containing solution but disappeared within minutes after excision or immediately upon exposure to Ca2+-free solution and were not reactivated upon reapplication of Ca2+-containing solution. Channel activity was restored by application of purified recombinant Ca2+-CaM or exposure to Ca2+-free CaM followed by application of Ca2+-containing solution. Coexpression of the double charge reversal E84R/E87K in CaM (CaM:84/87) with SK2:64/67 reconstituted stable Ca2+-dependent channel activity that was not lost with exposure to Ca2+-free solution. Therefore, Ca2+-independent interactions with CaM are required for surface expression of SK channels, whereas the constitutive association between the two channel subunits is not an essential requirement for gating.
Constitutive Dimerization of Glycoprotein VI (GPVI) in Resting Platelets Is Essential for Binding to Collagen and Activation in Flowing Blood*

Background: Platelet collagen receptor GPVI likely functions as a dimer rather than a monomer. Results: Preformed GPVI dimers, but not monomers, in resting platelets bind specific collagen sequences and are essential for platelet adhesion and activation. Conclusion: Constitutive GPVI dimers on resting platelets support platelet adhesion to collagen and activation. Significance: Resting platelets bind collagen through GPVI dimers, allowing immediate initiation of thrombus formation. The platelet collagen receptor glycoprotein VI (GPVI) has been suggested to function as a dimer, with increased affinity for collagen. Dissociation constants (Kd) obtained by measuring recombinant GPVI binding to collagenous substrates showed that GPVI dimers bind with high affinity to tandem GPO (Gly-Pro-Hyp) sequences in collagen, whereas the markedly lower affinity of the monomer for all substrates implies that it is not the collagen-binding form of GPVI. Dimer binding required a high density of immobilized triple-helical (GPO)10-containing peptide, suggesting that the dimer binds multiple, discrete peptide helices. Differential inhibition of dimer binding by dimer-specific antibodies, m-Fab-F and 204-11 Fab, suggests that m-Fab-F binds at the collagen-binding site of the dimer, and 204-11 Fab binds to a discrete site. Flow cytometric quantitation indicated that GPVI dimers account for ∼29% of total GPVI in resting platelets, whereas activation by either collagen-related peptide or thrombin increases the number of dimers to ∼39 and ∼44%, respectively. m-Fab-F inhibits both GPVI-dependent static platelet adhesion to collagen and thrombus formation on collagen under low and high shear, indicating that pre-existing dimeric GPVI is required for the initial interaction with collagen because affinity of the monomer is too low to support binding and that interaction through the dimer is essential for platelet activation. These GPVI dimers in resting circulating platelets will enable them to bind injury-exposed subendothelial collagen to initiate platelet activation. The GPVI-specific agonist collagen-related peptide or thrombin further increases the number of dimers, thereby providing a feedback mechanism for reinforcing binding to collagen and platelet activation.
Coupling novelty and surprise for evolutionary divergence

Divergent search techniques applied to evolutionary computation, such as novelty search and surprise search, have demonstrated their efficacy in highly deceptive problems compared to traditional objective-based fitness evolutionary processes. While novelty search rewards unseen solutions, surprise search rewards unexpected solutions. As a result these two algorithms perform a different form of search since an expected solution can be novel while an already seen solution can be surprising. As novelty and surprise search have already shown much promise individually, the hypothesis is that an evolutionary process that rewards both novel and surprising solutions will be able to handle deception in a better fashion and lead to more successful solutions faster. In this paper we introduce an algorithm that realises both novelty and surprise search and we compare it against the two algorithms that compose it in a number of robot navigation tasks. The key findings of this paper suggest that coupling novelty and surprise is advantageous compared to each search approach on its own. The introduced algorithm breaks new ground in divergent search as it outperforms both novelty and surprise in terms of efficiency and robustness, and it explores the behavioural space more extensively.
Asthma in adult life--is there an association with birth weight?

OBJECTIVE To determine whether birth weight is associated with asthmatic disorders in adult life. DESIGN A case-control study which compared birth weight and other indicators of pregnancy outcome in a group of adult asthmatic patients and in matched healthy controls. The diagnosis of asthma was verified using defined criteria. All asthmatic subjects were excluded from the control group. SETTING Four primary health care centres in Lund, Sweden. SUBJECTS 55 asthma patients and 92 healthy controls, all born between 1948 and 1973 in Lund. MAIN OUTCOME MEASURES Birth weight, birth height, head circumference, placental weight, and gestational age. Data from a self-administered questionnaire on social and medical background factors including smoking, educational level, and a self-reported history of frequent severe respiratory infections in childhood. RESULTS No significant differences were found between asthma patients and controls in birth weight, 3529 (SD 533) g versus 3536 (501) g, or in other variables associated with pregnancy outcome. There were no differences in current smoking habits or in educational level between the groups. The asthma patients, however, reported a higher number of childhood respiratory infections (p < 0.001) than the controls. CONCLUSIONS These epidemiological findings do not lend support to the hypothesis that a reduced birth weight is associated with a "programming" factor for asthma. Environmental factors in childhood seem more important than fetal malnutrition for the development of asthma in adult life.
A Comparative Study of Logic Programs with Preference: Preliminary Report

We are interested in semantical underpinnings for existing approaches to preference handling in extended logic programming (within the framework of answer set programming). As a starting point, we explore three different approaches that have been recently proposed in the literature. Because these approaches use rather different formal means, we furnish a series of uniform characterizations that allow us to gain insights into the relationships among these approaches. To be more precise, we provide different characterizations in terms of (i) fixpoints, (ii) order preservation, and (iii) translations into standard logic programs. While the two former provide semantics for logic programming with preference information, the latter furnishes implementation techniques for these approaches.
GCR1, the putative Arabidopsis G protein-coupled receptor gene is cell cycle-regulated, and its overexpression abolishes seed dormancy and shortens time to flowering

Although signaling through heterotrimeric G proteins has been extensively studied in eukaryotes, there is little information about this important signaling pathway in plants. We observed that expression of GCR1, the gene encoding the only known (but still putative) G protein-coupled receptor of Arabidopsis thaliana, is modulated during the cell cycle and during plant development. Overexpression of GCR1 in tobacco (Nicotiana tabacum) BY-2 cells caused an increase in thymidine incorporation and in the mitotic index of aphidicolin synchronized cells. Overexpression of GCR1 in Arabidopsis caused two remarkable phenotypes: seed dormancy was abolished and time to flowering was reduced. Molecular markers of these two developmental processes (phosphatase PP2A and MYB65 in germination; LFY during flowering) were up-regulated in GCR1 overexpressors. These data are consistent with the hypothesis that GCR1 may be a regulator of the cell cycle and that this regulation underlies the developmental changes observed in the GCR1 transformants.
Genetic diversity and haplotype distribution of Pachymeniopsis gargiuli sp. nov. and P. lanceolata (Halymeniales, Rhodophyta) in Korea, with notes on their non‐native distributions

The red alga Pachymeniopsis lanceolata, formerly known as Grateloupia lanceolata, is a component of the native algal flora of northeast Asia and has been introduced to European and North American waters. It has been confused with a cryptic species collected from Korea and Italy. Our analyses of rbcL, cox3 and ITS from P. lanceolata and this cryptic species has revealed two distinct entities, forming a clade, which were clearly separated from its congeners and positioned with other Asian species. Here, we describe the cryptic species as P. gargiuli sp. nov., a species that differs from others by molecular sequence and subtle anatomical characters. We hypothesize that P. gargiuli may have been recently dispersed by anthropogenic vectors, possibly at or near the same time as was P. lanceolata. Our cox3 data set revealed that one haplotype of P. gargiuli, shared between Korea and Italy, and two haplotypes of P. lanceolata, commonly occurring in Korea and USA, are invasive haplotypes. This is the first report of the utility of the mitochondrial coding cox3 sequences in red algae.