Miracle-dz/UniFAR-FTUs huggingface dataset:
Frontostriatal Circuit Dynamics Correlate with Cocaine Cue-Evoked Behavioral Arousal during Early Abstinence123

Visual Overview It is thought that frontostriatal circuits play an important role in mediating conditioned behavioral responses to environmental stimuli that were previously encountered during drug administration. However, the neural correlates of conditioned responses to drug-associated cues are not well understood at the level of large populations of simultaneously recorded neurons, or at the level of local field potential (LFP) synchrony in the frontostriatal network. Here we introduce a behavioral assay of conditioned arousal to cocaine cues involving pupillometry in awake head-restrained mice. After just 24 h of drug abstinence, brief exposures to olfactory stimuli previously paired with cocaine injections led to a transient dilation of the pupil, which was greater than the dilation effect to neutral cues. In contrast, there was no cue-selective change in locomotion, as measured by the rotation of a circular treadmill. The behavioral assay was combined with simultaneous recordings from dozens of electrophysiologically identified units in the medial prefrontal cortex (mPFC) and ventral striatum (VS). We found significant relationships between cocaine cue-evoked pupil dilation and the proportion of inhibited principal cells in the mPFC and VS. Additionally, LFP coherence analysis revealed a significant correlation between pupillary response and synchrony in the 25–45 Hz frequency band. Together, these results show that pupil dilation is sensitive to drug-associated cues during acute stages of abstinence, and that individual animal differences in this behavioral arousal response can be explained by two complementary measures of frontostriatal network activity.
Role of annexin A 6 in cancer ( Review )

Annexin A6 (AnxA6) is a member of a conserved superfamily of Ca2+-dependent membrane-binding annexin proteins. It participates in membrane and cytoskeleton organization, cholesterol homeostasis, membrane trafficking, cell adhesion and signal transduction. The expression levels of AnxA6 are closely associated with melanoma, cervical cancer, epithelial carcinoma, breast cancer, gastric cancer, prostate cancer, acute lymphoblastic leukemia, chronic myeloid leukemia, large-cell lymphoma and myeloma. AnxA6 exhibits dual functions in cancer, acting either as a tumor suppressor or promoter, depending on the type of cancer and the degree of malignancy. In several types of cancer, AnxA6 acts via Ras, Ras/MAPK and/or FAK/PI3K signaling pathways by mainly mediating PKCα, p120GAP, Bcr-Abl and YY1. In the present review, the roles of AnxA6 in different types of cancer are summarized.
Electrical and mechanical characterization of lateral NEMS Switches

In this paper we present a study on the electrical and mechanical characterization of NEMS Nano Switches. Pull-in, pull-out voltages are in good agreement with the theoretical values. However some reliability and sticking problems are identified. To investigate furthermore the mechanical properties of the nanoswitch (NS) beam, we have developed a dedicated AFM methodology to extract the Young's modulus. A further improvement of the design is then realized based on the results of this study.
Optimal Versus Naive Diversification: How Inefficient is the 1/N Portfolio Strategy?

We evaluate the out-of-sample performance of the sample-based mean-variance model, and its extensions designed to reduce estimation error, relative to the naive 1-N portfolio. Of the 14 models we evaluate across seven empirical datasets, none is consistently better than the 1-N rule in terms of Sharpe ratio, certainty-equivalent return, or turnover, which indicates that, out of sample, the gain from optimal diversification is more than offset by estimation error. Based on parameters calibrated to the US equity market, our analytical results and simulations show that the estimation window needed for the sample-based mean-variance strategy and its extensions to outperform the 1-N benchmark is around 3000 months for a portfolio with 25 assets and about 6000 months for a portfolio with 50 assets. This suggests that there are still many "miles to go" before the gains promised by optimal portfolio choice can actually be realized out of sample. The Author 2007. Published by Oxford University Press on behalf of The Society for Financial Studies. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org, Oxford University Press.
Complex Semidefinite Programming and Max-k-Cut

In a second seminal paper on the application of semidefinite programming to graph partitioning problems, Goemans and Williamson showed in 2004 how to formulate and round a complex semidefinite program to give what is to date still the best-known approximation guarantee of . 836008 for Max-3-Cut. (This approximation ratio was also achieved independently around the same time by De Klerk et al.) Goemans and Williamson left open the problem of how to apply their techniques to Max-k-Cut for general k. They point out that it does not seem straightforward or even possible to formulate a good quality complex semidefinite program for the general Max-k-Cut problem, which presents a barrier for the further application of their techniques. We present a simple rounding algorithm for the standard semidefinite programmming relaxation of Max-k-Cut and show that it is equivalent to the rounding of Goemans and Williamson in the case of Max-3-Cut. This allows us to transfer the elegant analysis of Goemans and Williamson for Max-3-Cut to Max-k-Cut. For k > 3, the resulting approximation ratios are about . 01 worse than the best known guarantees. Finally, we present a generalization of our rounding algorithm and conjecture (based on computational observations) that it matches the best-known guarantees of De Klerk et al.
Evolving controllers for simulated car racing

This paper describes the evolution of controllers for racing a simulated radio-controlled car around a track, modelled on a real physical track. Five different controller architectures were compared, based on neural networks, force fields and action sequences. The controllers use egocentric (first person), Newtonian (third person) or no information about the state of the car (open-loop controller). The only controller that able to evolve good racing behaviour was based on neural network acting on egocentric inputs.
Prognostic Classifier Based on Genome-Wide DNA Methylation Profiling in Well-Differentiated Thyroid Tumors

Context: Even though the majority of well‐differentiated thyroid carcinoma (WDTC) is indolent, a number of cases display an aggressive behavior. Cumulative evidence suggests that the deregulation of DNA methylation has the potential to point out molecular markers associated with worse prognosis. Objective: To identify a prognostic epigenetic signature in thyroid cancer. Design: Genome‐wide DNA methylation assays (450k platform, Illumina) were performed in a cohort of 50 nonneoplastic thyroid tissues (NTs), 17 benign thyroid lesions (BTLs), and 74 thyroid carcinomas (60 papillary, 8 follicular, 2 Hürthle cell, 1 poorly differentiated, and 3 anaplastic). A prognostic classifier for WDTC was developed via diagonal linear discriminant analysis. The results were compared with The Cancer Genome Atlas (TCGA) database. Results: A specific epigenetic profile was detected according to each histological subtype. BTLs and follicular carcinomas showed a greater number of methylated CpG in comparison with NTs, whereas hypomethylation was predominant in papillary and undifferentiated carcinomas. A prognostic classifier based on 21 DNA methylation probes was able to predict poor outcome in patients with WDTC (sensitivity 63%, specificity 92% for internal data; sensitivity 64%, specificity 88% for TCGA data). High‐risk score based on the classifier was considered an independent factor of poor outcome (Cox regression, P < 0.001). Conclusions: The methylation profile of thyroid lesions exhibited a specific signature according to the histological subtype. A meaningful algorithm composed of 21 probes was capable of predicting the recurrence in WDTC.
Ultrastructural characterization of apoptotic granulosa cells in caprine ovary

The unique phenomenon of cell proliferation and apoptosis is encountered in the ovarian follicles undergoing early stages of atresia. The aim of this study was to verify the morphological variations in these two physiologically distinct processes operating in antral follicles of caprine ovaries using histological and ultrastructural techniques. Histologically the degenerating granulosa cells were characterized by condensed cytoplasm, and nucleus fragmentation in hazy cytosol. The pyknotic nuclei of degenerating cells stained darkly with haematoxylin and giemsa while the cytoplasm was eosinophilic. Under electron microscopy, apoptosis was marked by asymmetrical shrinkage, vacuolization of cytoplasm, swollen and vacuolated mitochondria, increased irregularity and/or fragmentation of nucleus, chromatin condensation and finally, production of membrane enclosed nuclear fragments containing intracellular material, the apoptotic bodies. The parallel use of these two methods on caprine ovaries has enabled us to analyse the decline in the frequency of granulosa cells during follicular atresia due to apoptosis.
Do artificial velocity refuges mitigate the physiological and behavioural consequences of hydropeaking on a freshwater Iberian cyprinid?

The rapid flow fluctuations experienced downstream of hydropeaking facilities can alter the river hydromorphology. Given the dependence of riverine fish on physical habitat, those alterations have the potential to change the physiology and behaviour of fish. We assessed whether artificial velocity refuges mitigated the physiological and behavioural consequences of hydropeaking for the Iberian barbel (Luciobarbus bocagei). Hydropeaking trials were conducted in an indoor flume equipped with deflectors that created low flow velocity areas to serve as refuges. The FLOW‐3D was used to obtain detailed characterizations of the different velocity fields, which facilitated the interpretation of fish responses. Changes in flow magnitude and duration triggered stress responses, demonstrated by the increased blood glucose levels in the single up‐ramping event for 60 L s−1 and in the step up‐ramping event. Fish tended to seek out velocity refuges to avoid higher flow velocities and harsh hydraulic conditions at peak flows, and during the longer events. The movement behaviour frequency increased when fish were subjected to the highest peak flow (60 L s−1), particularly the individual sprints and the drifts. For the base flow (7 L s−1) and the lowest peak flow (20 L s−1) conditions, fish swam freely in the flume, whereas in the harshest hydraulic conditions they showed more difficulty in finding velocity refuges. This research presents a novel approach by combining physiology and behavioural observations with hydraulic modelling to assess the extent to which artificial flow refuges mitigate the consequences of hydropeaking. Our work serves as a model approach for future mitigation studies for fish in hydropeaking rivers.
Static and Dynamic Crystalline Lens Accommodation Evaluated Using Quantitative 3-d Oct References and Links Anterior Segment Imaging with Spectral Oct System Using a High-speed Cmos Camera, " Opt

Custom high-resolution high-speed anterior segment spectral domain Optical Coherence Tomography (OCT) provided with automatic quantification and distortion correction algorithms was used to characterize three-dimensionally (3-D) the human crystalline lens in vivo in four subjects, for accommodative demands between 0 to 6 D in 1 D steps. Anterior and posterior lens radii of curvature decreased with accommodative demand at rates of 0.73 and 0.20 mm/D, resulting in an increase of the estimated optical power of the eye of 0.62 D per diopter of accommodative demand. Dynamic fluctuations in crystalline lens radii of curvature, anterior chamber depth and lens thickness were also estimated from dynamic 2-D OCT images (14 Hz), acquired during 5-s of steady fixation, for different accommodative demands. Estimates of the eye power from dynamical geometrical measurements revealed an increase of the fluctuations of the accommodative response from 0.07 D to 0.47 D between 0 and 6 D (0.044 D per D of accommodative demand). A sensitivity analysis showed that the fluctuations of accommodation were driven by dynamic changes in the lens surfaces, particularly in the posterior lens surface. A comparison study of dynamic retinoscopy techniques, " Optom. Dynamics of the eye's wave aberration, " J. Opt. The effect of ocular aberrations on steady-state errors of accommodative response, " J. Adaptive optics system for investigation of the effect of the aberration dynamics of the human eye on steady-state accommodation control, " J. Accommodative lag and fluctuations when optical aberrations are manipulated, " J. Accommodative microfluctuations and pupil diameter, " Vision Res. A computational model of the error detector of human visual accommodation, " Biol. Accommodation and presbyopia in the human eye. Changes in the anterior segment and crystalline lens with focus, " Invest. Scheimpflug and high-resolution magnetic resonance imaging of the anterior segment: a comparative study, " J. Change in shape of the aging human crystalline lens with accommodation, " Vision Res. MRI study of the changes in crystalline lens shape with accommodation and aging in humans, " J. Dynamic accommodative changes in rhesus monkey eyes assessed with A-scan ultrasound biometry, " Optom. high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range, " Opt. Accuracy of cornea and lens biometry using anterior segment optical coherence tomography, " J. Scanning system design for large scan depth anterior segment optical coherence tomography, " Opt.OCT with prolonged scan depth for imaging the anterior …
Intracontinental Orogeny Enhanced by Far‐Field Extension and Local Weak Crust

The accommodation of intraplate stresses in preexisting weak regions of plate interiors is here investigated using thin viscous sheet numerical models. The intraplate stresses are governed by multicomponent and multidirectional stresses originating at plate boundaries. The modeled scenarios mimic plate boundary conditions during the intraplate Alice Springs Orogeny (ASO), central Australia, and include (1) a northwest‐southeast zone of weak lithosphere within strong continental blocks to the north and southand (2) a principal south directed stress condition at the northern boundary that causes minor clockwise rotation of the northern block. Alternative tectonic environments are investigated in additional models that include (1) secondary compressional or extensional stresses acting at the eastern boundary, representing the temporally variable stress conditions during the Tasmanides Orogeny, and (2) an eastern wedge‐shaped zone of rheologically weak lithosphere, mirroring rift fill of the Irindina subbasin. Our results highlight that a low angle between major crustal features (e.g., orogenic elongation and preexisting regional structures) and the principal transmitted stresses is highly relevant in the concentration of elevated levels of differential stress and subsequent localization of deformation in plate interiors. Secondary stresses orthogonal to the principal acting stresses may introduce effects that explain the episodic orogenic activity in the case of the ASO. The combination of secondary extensional stresses at the eastern boundary of Australia and weak lithosphere of the preexisting Irindina subbasin strongly influences the observed spatial strain intensity, localization, and kinematics of deformation during the ASO.
Gorillas in Our Midst: Sustained Inattentional Blindness for Dynamic Events

With each eye fixation, we experience a richly detailed visual world. Yet recent work on visual integration and change direction reveals that we are surprisingly unaware of the details of our environment from one view to the next: we often do not detect large changes to objects and scenes (‘change blindness’). Furthermore, without attention, we may not even perceive objects (‘inattentional blindness’). Taken together, these findings suggest that we perceive and remember only those objects and details that receive focused attention. In this paper, we briefly review and discuss evidence for these cognitive forms of ‘blindness’. We then present a new study that builds on classic studies of divided visual attention to examine inattentional blindness for complex objects and events in dynamic scenes. Our results suggest that the likelihood of noticing an unexpected object depends on the similarity of that object to other objects in the display and on how difficult the priming monitoring task is. Interestingly, spatial proximity of the critical unattended object to attended locations does not appear to affect detection, suggesting that observers attend to objects and events, not spatial positions. We discuss the implications of these results for visual representations and awareness of our visual environment.
Increased Hydration Can Be Associated with Weight Loss

This mini-review develops the hypothesis that increased hydration leads to body weight loss, mainly through a decrease in feeding, and a loss of fat, through increased lipolysis. The publications cited come from animal, mainly rodent, studies where manipulations of the central and/or the peripheral renin–angiotensin system lead to an increased drinking response and a decrease in body weight. This hypothesis derives from a broader association between chronic hypohydration (extracellular dehydration) and raised levels of the hormone angiotensin II (AngII) associated with many chronic diseases, such as obesity, diabetes, cancer, and cardiovascular disease. Proposed mechanisms to explain these effects involve an increase in metabolism due to hydration expanding cell volume. The results of these animal studies often can be applied to the humans. Human studies are consistent with this hypothesis for weight loss and for reducing the risk factors in the development of obesity and type 2 diabetes.
Community-based outpatient parenteral antimicrobial therapy (CoPAT) for Staphylococcus aureus bacteraemia with or without infective endocarditis: analysis of the randomized trial comparing daptomycin with standard therapy

Objectives Administering outpatient parenteral antimicrobial therapy in the community setting (CoPAT) is becoming more common with the increasing emphasis on controlling costs. However, few controlled trials have evaluated this treatment modality. Methods Using data from a recent randomized trial comparing daptomycin with standard therapy (semi-synthetic penicillin or vancomycin, each with initial low-dose gentamicin) for Staphylococcus aureus bacteraemia and infective endocarditis (SAB/IE), patient characteristics and outcomes were evaluated. Patients receiving their full course of therapy in the hospital setting were compared with those who received some portion outside of the hospital (CoPAT). Results Among the 200 patients, 51.5% received CoPAT. These patients were generally younger (median age 50 versus 54 years, P = 0.028). In the CoPAT group, there tended to be fewer patients with endocardial involvement (8.7% versus 18.6%, P = 0.061) and pre-existing valvular heart disease (7.8% versus 15.5%, P = 0.120). CoPAT patients received longer therapy courses (mean 25.4 versus 13.5 days, P < 0.001) and had higher rates of therapy completion (90.3% versus 45.4%, P < 0.001) and clinical success (86.4% versus 55.7%, P < 0.001). Persisting or relapsing S. aureus was less frequent in the CoPAT group (3.9% versus 15.5%, P = 0.007) and there were fewer deaths (3.9% versus 18.6%, P = 0.001) 6 weeks after the end of therapy. Hospital readmission occurred for 18 of the 103 (17.5%) CoPAT patients. Clinical success rates were similar for CoPAT patients receiving daptomycin (90.0%) or standard therapy (83.0%). Conclusions With proper monitoring, stable patients can complete treatment for SAB/IE as outpatients in the community setting. Daptomycin is an appropriate option for this setting.
Comparative benefits of laparoscopic vs open hepatic resection: a critical appraisal.

OBJECTIVES To perform a literature review examining the comparative benefits of laparoscopic vs open hepatic resection and to define the benefits and outcomes of laparoscopic liver resection in our own series of 314 patients. DATA SOURCES Cited English-language publications from PubMed. In addition, between 2001 to 2010, hepatic resections were performed in our institution in 1294 patients, of whom 314 patients (24.3%) underwent laparoscopic liver resection for benign or malignant liver lesions. STUDY SELECTION Search phrases were "laparoscopic liver resection," "open liver resection," "versus," "compared with," and "advantages. " DATA EXTRACTION Thirty-one studies were reviewed that directly compared laparoscopic with open hepatic resection in 2473 patients. DATA SYNTHESIS In case-cohort matched studies, and our institutional series, laparoscopic liver resection was associated with less blood loss, quicker resumption of oral diet, less pain medication requirement, and shorter length of stay, with no difference in complication rates. In those patients undergoing laparoscopic hepatic resection for malignancy, there was no difference in 3- or 5-year overall survival when compared with well-matched open hepatic resection cases. Financially, the total hospital costs of laparoscopic liver resection were either offset or improved because of a shorter length of stay. CONCLUSIONS Based on review of the literature and our institutional series, minimally invasive hepatic resection for benign and malignant liver lesions is safe and feasible with significant benefits for patients consisting of less blood loss, less narcotic requirements, and shorter length of hospital stay. There are no economic disadvantages to the laparoscopic approach, and case-cohort matched studies show no difference in oncologic outcomes between the laparoscopic and open groups.
Simultaneous detection and differentiation of human polyomaviruses JC and BK by a rapid and sensitive PCR‐ELAHA assay and a survey of the JCV subtypes within an Australian population

Human polyomaviruses JCV and BKV can cause several clinical manifestations in immunocompromised hosts, including progressive multifocal leukoencephalopathy (PML) and haemorrhagic cystitis. Molecular detection by polymerase chain reaction (PCR) is recognised as a sensitive and specific method for detecting human polyomaviruses in clinical samples. In this study, we developed a PCR assay using a single primer pair to amplify a segment of the VP1 gene of JCV and BKV. An enzyme linked amplicon hybridisation assay (ELAHA) using species‐specific biotinylated oligonucleotide probes was used to differentiate between JCV and BKV. This assay (VP1‐PCR‐ELAHA) was evaluated and compared to a PCR assay targeting the human polyomavirus T antigen gene (pol‐PCR). DNA sequencing was used to confirm the polyomavirus species identified by the VP1‐PCR‐ELAHA and to determine the subtype of each JCV isolate. A total of 297 urine specimens were tested and human polyomavirus was detected in 105 specimens (35.4%) by both PCR assays. The differentiation of JCV and BKV by the VP1‐PCR‐ELAHA showed good agreement with the results of DNA sequencing. Further, DNA sequencing of the JCV positive specimens showed the most prevalent JCV subtype in our cohort was 2a (27%) followed by 1b (20%), 1a (15%), 2c (14%), 4 (14%) and 2b (10%). The results of this study show that the VP1‐PCR‐ELAHA is a sensitive, specific and rapid method for detecting and differentiating human polyomaviruses JC and BK and is highly suitable for routine use in the clinical laboratory. J. Med. Virol. 72:467–472, 2004. © 2004 Wiley‐Liss, Inc.