Miracle-dz/UniFAR-FTUs huggingface dataset:
Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody

Cultured mononuclear peripheral blood leukocytes (PBL) from nonimmune human beings and monkeys are nonpermissive to dengue 2 virus (D2V) infection at multiplicities of infection of 0.001-0.1, but become permissive when non-neutralizing dengue antibody is added to medium. D2V infection occurred in PBL prepared from anti-coagulated but not from defibrinated plasma. Infection enhancement was produced by multiple lots of heterotypic anti-dengue raised in several mammalian species. Homotypic anti-dengue neutralized D2V at high concentrations but enhanced at low concentrations; enhancement end point in one serum was 1:320,000. The infection-enhancing factor was a noncytophilic antibody of the IgG class. D2V infection occurred in the absence of heat-labile complement components but did not occur when complexes were prepared with anti- dengue F(ab)(2). Treatment of PBL with several proteases increased permissiveness to D2V infection by immune complexes but not by virus alone. Two rhesus monkey serums collected 14 days after D2V infection contained an IgG antibody with high-titered enhancing activity but with no hemagglutination-inhibition or neutralizing activity. Virus-antibody complexes are irreversibly attached to PBL within 15 min and completely internalized in 60 min. There was considerable variation in cellular infection in different experiments, however, maximum virus yields usually exceeded 1,000 plaque-forming units per 1 x 10(6) PBL occurring between 2 and 4 days in culture. In vitro antibody-dependent infection of PBL provides a possible model for study of pathogenetic mechanisms in infants with dengue shock syndrome who passively acquire maternal anti-dengue IgG.
Human mesenchymal stem cells as a two-edged sword in hepatic regenerative medicine: engraftment and hepatocyte differentiation versus profibrogenic potential

Background and aim: Mesenchymal stem cells from bone marrow (MSCs) may have the potential to differentiate in vitro and in vivo into hepatocytes. We investigated whether transplanted human MSCs (hMSCs) may engraft the liver of non-obese diabetic severe combined immuno-deficient (NOD/SCID) mice and differentiate into cells of hepatic lineage. Methods: Ex vivo expanded, highly purified and functionally active hMSCs from bone marrow were transplanted (caudal vein) in sublethally irradiated NOD/SCID mice that were either exposed or not to acute liver injury or submitted to a protocol of chronic injury (single or chronic intraperitoneal injection of CCl4, respectively). Chimeric livers were analysed for expression of human transcripts and antigens. Results: Liver engraftment of cells of human origin was very low in normal and acutely injured NOD/SCID mice with significantly higher numbers found in chronically injured livers. However, hepatocellular differentiation was relatively rare, limited to a low number of cells (ranging from less than 0.1% to 0.23%) as confirmed by very low or not detectable levels of human transcripts for α-fetoprotein, CK18, CK19 and albumin in either normal or injured livers. Finally, a significant number of cells of human origin exhibited a myofibroblast-like morphology. Conclusions: Transplanted hMSCs have the potential to migrate into normal and injured liver parenchyma, particularly under conditions of chronic injury, but differentiation into hepatocyte-like cells is a rare event and pro-fibrogenic potential of hMSC transplant should be not under-evaluated.
ACCOMMODATING BODIES: CELEBRITY, SEXUALITY, AND “TRAGIC MAGIC”

The media coverage of U.S. basketball star Magic Johnson's revelation of his HIV status and the unfolding of the “Magic Johnson Story” invokes and traverses a range of discourses, including the medical, the sexual, the racial, and the “celebritorial. ” A series of discursive contradictions and boundary confusions is thereby produced in the domain of media sport. This combination of significatory and ideological possibilities provides an opportunity to examine the making—and potential unmaking—of (a) celebrity. In the process, some of the more obscure structures and processes of social power, exercised through the construction of the body and its various dispositions, come into focus. The narrativized predicament of Magic Johnson is used here to interrogate how particular constructions of the body may be enlisted in the domain of popular culture, invested and inscribed by the media with ideologies of dominance, and deployed to deter disruptive, antihegemonic readings of discomfiting social phenomena. Viewed in this manner, the sporting body emerges as a key site of politico-cultural contestation, its celebration inevitably accompanied by its dissection.
Validation of a Risk Stratification Index and Risk Quantification Index for Predicting Patient Outcomes: In-hospital Mortality, 30-day Mortality, 1-year Mortality, and Length-of-stay

Background:External validation of published risk stratification models is essential to determine their generalizability. This study evaluates the performance of the Risk Stratification Indices (RSIs) and 30-day mortality Risk Quantification Index (RQI). Methods:108,423 adult hospital admissions with anesthetics were identified (2006–2011). RSIs for mortality and length-of-stay endpoints were calculated using published methodology. 91,128 adult, noncardiac inpatient surgeries were identified with administrative data required for RQI calculation. Results:RSI in-hospital mortality and RQI 30-day mortality Brier scores were 0.308 and 0.017, respectively. RSI discrimination, by area under the receiver operating curves, was excellent at 0.966 (95% CI, 0.963–0.970) for in-hospital mortality, 0.903 (0.896–0.909) for 30-day mortality, 0.866 (0.861–0.870) for 1-yr mortality, and 0.884 (0.882–0.886) for length-of-stay. RSI calibration, however, was poor overall (17% predicted in-hospital mortality vs. 1.5% observed after inclusion of the regression constant) as demonstrated by calibration plots. Removal of self-fulfilling diagnosis and procedure codes (20,001 of 108,423; 20%) yielded similar results. RQIs were calculated for only 62,640 of 91,128 patients (68.7%) due to unmatched procedure codes. Patients with unmatched codes were younger, had higher American Society of Anesthesiologists physical status and 30-day mortality. The area under the receiver operating curve for 30-day mortality RQI was 0.888 (0.879–0.897). The model also demonstrated good calibration. Performance of a restricted index, Procedure Severity Score + American Society of Anesthesiologists physical status, performed as well as the original RQI model (age + American Society of Anesthesiologists + Procedure Severity Score). Conclusion:Although the RSIs demonstrated excellent discrimination, poor calibration limits their generalizability. The 30-day mortality RQI performed well with age providing a limited contribution.
Team Exploratory and Exploitative Learning: Psychological Safety, Task Conflict, and Team Performance

This study conceptualized exploratory and exploitative learning as distinct team-level activities, constructed measures of them, and examined their relationships with psychological safety, task conflict, and team performance. Structural equation analysis in a sample of 142 innovation project teams indicated that psychological safety was linearly and nonlinearly related to team exploitative and exploratory learning, respectively; whereas task conflict positively moderated the relationship between psychological safety and exploitative learning. Furthermore, exploratory and exploitative learning were additively related to team performance, as rated by team managers, and mediated its relationship with psychological safety. The findings contribute to understanding how and under what conditions organizational teams engage in exploratory and exploitative learning to maximize their performance.
Neuroligin-1 Loss Is Associated with Reduced Tenacity of Excitatory Synapses

Neuroligins (Nlgns) are postsynaptic, integral membrane cell adhesion molecules that play important roles in the formation, validation, and maturation of synapses in the mammalian central nervous system. Given their prominent roles in the life cycle of synapses, it might be expected that the loss of neuroligin family members would affect the stability of synaptic organization, and ultimately, affect the tenacity and persistence of individual synaptic junctions. Here we examined whether and to what extent the loss of Nlgn-1 affects the dynamics of several key synaptic molecules and the constancy of their contents at individual synapses over time. Fluorescently tagged versions of the postsynaptic scaffold molecule PSD-95, the AMPA-type glutamate receptor subunit GluA2 and the presynaptic vesicle molecule SV2A were expressed in primary cortical cultures from Nlgn-1 KO mice and wild-type (WT) littermates, and live imaging was used to follow the constancy of their contents at individual synapses over periods of 8–12 hours. We found that the loss of Nlgn-1 was associated with larger fluctuations in the synaptic contents of these molecules and a poorer preservation of their contents at individual synapses. Furthermore, rates of synaptic turnover were somewhat greater in neurons from Nlgn-1 knockout mice. Finally, the increased GluA2 redistribution rates observed in neurons from Nlgn-1 knockout mice were negated by suppressing spontaneous network activity. These findings suggest that the loss of Nlgn-1 is associated with some use-dependent destabilization of excitatory synapse organization, and indicate that in the absence of Nlgn-1, the tenacity of excitatory synapses might be somewhat impaired.
Cultural aspects of anxiety disorders in India

Cultural factors have influenced the presentation, diagnoses, and treatment of anxiety disorders in India for several centuries. This review covers the antecedents, prevalence, phenomenology, and treatment modalities of anxiety disorders in the Indian cultural context. It covers the history of the depiction of anxiety in India and the concept of culture in the classification of anxiety disorders, and examines the cultural factors influencing anxiety disorders in India. We review the prevalence and phenomenology of various disorders, such as generalized anxiety disorder, panic disorder, social anxiety, and phobic disorder, as well as culture-specific syndromes such as dhat and koro in India. Finally, the review examines the wide range of therapeutic modalities practiced in India, such as faith healing, psychotherapy, ayurveda, psychopharmacology, Unani medicine, homeopathy, yoga, meditation, and mindfulness. We conclude by emphasizing the significance of cultural factors in making relevant diagnoses and offering effective and holistic treatments to individuals with anxiety disorders.
The significance of vortex ring formation to the impulse and thrust of a starting jet

The recent work of Gharib, Rambod, and Shariff [J. Fluid Mech. 360, 121 (1998)] studied vortex rings formed by starting jets generated using a piston-cylinder mechanism. Their results showed that vortex rings generated from starting jets stop forming and pinch off from the generating jet for sufficiently large values of the piston stroke to diameter ratio (L/D), suggesting a maximization principle may exist for propulsion utilizing starting jets. The importance of vortex ring formation and pinch off to propulsion, however, rests on the relative contribution of the leading vortex ring and the trailing jet (which appears after pinch off) to the impulse supplied to the flow. To resolve the relative importance of the vortex ring and trailing jet for propulsion, a piston-cylinder mechanism attached to a force balance is used to investigate the impulse and thrust generated by starting jets for L/D ratios in the range 2–8. Two different velocity programs are used, providing two different L/D values beyond which pinch off is observed, in order to determine the effect of vortex ring pinch off. Measurements of the impulse associated with vortex ring formation show it to be much larger than that expected from the jet velocity alone and proportionally larger than that associated with a trailing jet for L/D large enough to observe pinch off. The latter result leads to a local maximum in the average thrust during a pulse near L/D values associated with vortex rings whose circulation has been maximized. These results are shown to be related to the nozzle exit over-pressure generated during vortex ring formation. The over-pressure is in turn shown to be associated with the acceleration of ambient fluid by vortex ring formation in the form of added and entrained mass.
Assessing the Impact of Harassment by Peers: Incident Characteristics and Outcomes in a National Sample of Youth

Although there are widely held assumptions about the characteristics of peer bullying that are of greatest concern, very few studies have empirically assessed which characteristics most affect its impact. The current research addresses this gap by using a nationally representative U.S. sample of youth ages 10–20 to examine the relative effects of a variety of potentially aggravating incident characteristics on emotional, physical health, and school-related outcomes. Findings show support for power imbalance and duration (a stronger predictor than repetition) as incident characteristics that exacerbate the negative impact of peer harassment. However, several other incident characteristics have substantial effects with or without the presence of these qualities. Injury, sexual content, involvement of multiple perpetrators, and hate/bias components of peer harassment incidents each increased at least one negative outcome. Findings point to several features of peer harassment that can provide a basis for prioritizing victimization experiences in greatest need of intervention efforts.
Optimal Crowds Contest Model for Crowdsourcing

With the increasing frequency of participation in social networking activities, tremendous value has been created by crowds. Thus some emerging industries come along with it to collect these values. At the same time, crowds require some compensation from the these project organizers for their privacy loss or cost of activities. This paper dedicate to exploit a users incentives system, it develops a game-theoretic model of crowdsourcing or crowdsensing services base on contests. The model consists of two parts: incentives and optimizing pricing. We start from the crowds' point of view, committed to dig out their equilibrium strategies. Based on this, a bonus pool and expected rewards are demonstrated for the organizer and crowds respectively.
Literacy development in children with cerebral palsy

This thesis concerns the literacy difficulties of fifteen children with cerebral palsy (CP). The children were an opportunistic sample from two schools, and were initially selected on the basis that they had typical abilities in other school subjects. A review of the literature pertaining to the development of literacy and related aspects of cognitive development in typically-developing children and children with CP informed the development of the research strategy. The children's literacy, their general non-verbal and communication abilities, as well as a set of cognitive abilities that could be related to literacy impairments, were assessed. The findings revealed that most, but not all, of the children with CP had literacy difficulties with low scores in reading and spelling, and all the children had problems with handwriting. Standardised assessments showed that while the children had good verbal abilities, they had very low scores on a non-verbal test. The children with the most severe literacy difficulties also had the most problems with phonological processing. Almost all of the children had difficulties with visual and spatial perception; however the better readers had typical results in an assessment of visual sequential memory. Tests of working memory (WM) revealed a clear division between typical levels of performance on verbal recall measures, and very poor performance on the visual components of working memory tests. There were mixed results for the assessment of the central executive, but generally those children who were the more able in literacy had higher scores. Correlations suggested that visual sequential memory; phonological segmentation; verbal recall; and aspects of the central executive of working memory were most closely associated with the children's literacy abilities. Thus, the findings indicate that children with CP ha,ve specific cognitive impairments, including a new suggestion that poor visual sequential memory abilities could delay the development of reading and spelling skills.
DeficiencyinNa,K-ATPaseIsoformGenesAltersSpatial Learning,MotorActivity,andAnxietyinMice

SeveraldisordershavebeenassociatedwithmutationsinNa,K-ATPaseisoforms(rapid-onsetdystoniaparkinsonism,familialhemiplegic migraine type-2), as well as reduction in Na,K-ATPase content (depression and Alzheimer’s disease), thereby raising the issue of whetherhaploinsufficiencyoralteredenzymaticfunctioncontributetodiseaseetiology.Threeisoformsareexpressedinthebrain:the1 isoformisfoundinmanycelltypes,the2isoformispredominantlyexpressedinastrocytes,andthe3isoformisexclusivelyexpressed in neurons. Here we show that mice heterozygous for the 2 isoform display increased anxiety-related behavior, reduced locomotor activity, and impaired spatial learning in the Morris water maze. Mice heterozygous for the 3 isoform displayed spatial learning and memory deficits unrelated to differences in cued learning in the Morris maze, increased locomotor activity, an increased locomotor responsetomethamphetamine,anda40%reductioninhippocampalNMDAreceptorexpression.Incontrast,heterozygous1isoform mice showed increased locomotor response to methamphetamine and increased basal and stimulated corticosterone in plasma. The learning and memory deficits observed in the 2 and 3 heterozygous mice reveal the Na,K-ATPase to be an important factor in the functioning of pathways associated with spatial learning. The neurobehavioral changes seen in heterozygous mice suggest that these mousemodelsmaybeusefulinfutureinvestigationsoftheassociatedhumanCNSdisorders.
Sympathetic nerve activity and insulin in obese normotensive and hypertensive men.

The relationship between resting levels of muscle sympathetic nerve activity (MSA) and blood pressure is a matter of controversy. Body weight has recently been identified as an independent determinant of muscle sympathetic discharge, which may have influenced previous studies focused on MSA and mechanisms of hypertension. In the present study, we measured resting MSA and plasma insulin levels in 18 obese (body mass index, 32 +/- 4 kg/m2) (mean +/- SD), middle-aged (52 +/- 6 years), hypertensive (155 +/- 11/97 +/- 8 mm Hg) subjects and 16 age- and body mass index-matched normotensive control subjects. In the postabsorptive state, resting MSA was similar in the hypertensive and normotensive groups (43 +/- 4 versus 39 +/- 3 bursts per minute, 69 +/- 5 versus 64 +/- 5 bursts per 100 heart beats, P = NS) (mean +/- SEM) and did not correlate with either systolic or diastolic blood pressure. Weak but significant positive correlations were found between resting MSA and both fasting insulin levels (P < .05) and body mass index (P = .05) in hypertensive but not normotensive subjects. There was a strong positive correlation between fasting insulin and body mass index in both normotensive subjects and the entire study group (P < .005). Fasting insulin and body mass index correlated with diastolic blood pressure (P < .05) in the entire study group. In conclusion, a relationship between fasting insulin, body mass index, and blood pressure was confirmed, whereas only a weak correlation was found between MSA and fasting insulin in hypertensive but not normotensive subjects. The fact that MSA was similar in the two groups argues strongly against augmented MSA being important for the maintenance of hypertension, at least in middle-aged, obese men.
Clinical evaluation of post-extraction site wound healing.

AIM The aim of this prospective study was to evaluate the clinical pattern of post-extraction wound healing with a view to identify the types, incidence, and pattern of healing complications following non-surgical tooth extraction. STUDY DESIGN A total of 311 patients, who were referred for non-surgical (intra-alveolar) extractions, were included in the study. The relevant pre-operative information recorded for each patient included age and gender of the patient, indications for extraction, and tooth/teeth removed. Extractions were performed under local anesthesia with dental forceps, elevators, or both. Patients were evaluated on the third and seventh postoperative days for alveolus healing assessment. Data recorded were: biodata, day of presentation for alveolus healing assessment, day of onset of any symptoms, body temperature (degrees C) in cases of alveolus infection, and presence or absence of pain. RESULTS Two hundred eighty-two patients (282) with 318 extraction sites were evaluated for alveolus healing. Healing was uneventful in 283 alveoli (89%), while 35 alveoli (11%) developed healing complications. These complications were: localized osteitis 26 (8.2%); acutely infected alveolus 5 (1.6%); and an acutely inflamed alveolus 4 (1.2%). Females developed more complications than males (p=0.003). Most complications were found in molars (60%) and premolars (37.1%). Localized osteitis caused severe pain in all cases, while infected and inflamed alveolus caused mild or no pain. Thirty patients (12%) among those without healing complications experienced mild pain. CONCLUSIONS Most of the post-extraction alveoli healed uneventfully. Apart from alveolar osteitis (AO), post-extraction alveolus healing was also complicated by acutely infected alveoli and acutely inflamed alveoli. This study also demonstrated a painful alveolus is not necessarily a disturbance of post-extraction site wound healing; a thorough clinical examination must, therefore, be made to exclude any of the complications.
Permutation Generation Methods

This paper surveys the numerous methods tha t have been proposed for pe rmuta tmn enumera t ion by computer. The various algori thms which have been developed over the years are described in detail, and zmplemented in a modern ALc, oL-hke language. All of the algori thms are derived from one rumple control structure. The problems involved with implement ing the best of the algori thms on real computers are t reated m detail. Assembly-language programs are derived and analyzed fully. The paper is intended not only as a survey of permuta t ion generation methods, but also as a tutomal on how to compare a number of different algori thms for the same task
NovaNET's Effect on the Reading Achievement of At-Risk Middle School Students

NovaNET’s Effect on the Reading Achievement of At-Risk Middle School Students by Alice Harris Jackson MA, University of West Alabama, 2007 BS, Concordia College Selma, 2006 Doctoral Study Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Education Walden University December 2015