Miracle-dz/UniFAR-FTUs huggingface dataset:
Sleep Deprivation Impairs Spatial Retrieval but Not Spatial Learning in the Non-Human Primate Grey Mouse Lemur

A bulk of studies in rodents and humans suggest that sleep facilitates different phases of learning and memory process, while sleep deprivation (SD) impairs these processes. Here we tested the hypothesis that SD could alter spatial learning and memory processing in a non-human primate, the grey mouse lemur (Microcebus murinus), which is an interesting model of aging and Alzheimer's disease (AD). Two sets of experiments were performed. In a first set of experiments, we investigated the effects of SD on spatial learning and memory retrieval after one day of training in a circular platform task. Eleven male mouse lemurs aged between 2 to 3 years were tested in three different conditions: without SD as a baseline reference, 8 h of SD before the training and 8 h of SD before the testing. The SD was confirmed by electroencephalographic recordings. Results showed no effect of SD on learning when SD was applied before the training. When the SD was applied before the testing, it induced an increase of the amount of errors and of the latency prior to reach the target. In a second set of experiments, we tested the effect of 8 h of SD on spatial memory retrieval after 3 days of training. Twenty male mouse lemurs aged between 2 to 3 years were tested in this set of experiments. In this condition, the SD did not affect memory retrieval. This is the first study that documents the disruptive effects of the SD on spatial memory retrieval in this primate which may serve as a new validated challenge to investigate the effects of new compounds along physiological and pathological aging.
Using an Instrumented Drone to Probe Dust Devils on Oregon's Alvord Desert

Dust devils are low-pressure, small (many to tens of meters) convective vortices powered by surface heating and rendered visible by lofted dust. Dust devils occur ubiquitously on Mars, where they may dominate the supply of atmospheric dust, and since dust contributes significantly to Mars’ atmospheric heat budget, dust devils probably play an important role in its climate. The dust-lifting capacity of a devil likely depends sensitively on its structure, particularly the wind and pressure profiles, but the exact dependencies are poorly constrained. Thus, the exact contribution to Mars’ atmosphere remains unresolved. Analog studies of terrestrial devils have provided some insights into dust devil dynamics and properties but have been limited to near-surface (few meters) or relatively high altitude (hundreds of meters) sampling. Automated aerial vehicles or drones, combined with miniature, digital instrumentation, promise a novel and uniquely powerful platform from which to sample dust devils at a wide variety of altitudes. In this article, we describe a pilot study using an instrumented quadcopter on an active field site in southeastern Oregon, which (to our knowledge) has not previously been surveyed for dust devils. We present preliminary results from the encounters, including stereo image analysis and encounter footage collected onboard the drone. In spite of some technical difficulties, we show that a quadcopter can successfully navigate in an active dust devil, while collecting time-series data about the dust devil’s structure.
Geometric and Temperature Effects on Time Domain Reflectometry Measurements in Soils

Dallinger, Teresa Eileen. M.S.C.E. , Purdue University, December, 2006. Geometric and Temperature Effects on Time Domain Reflectometry Measurements in Soils. Major Professor: Vincent P. Drnevich. Time domain reflectometry (TDR) is a valuable tool used to measure the water content and dry density in soils. The success of TDR relies on the accurate measurements of the apparent dielectric constant (Ka) and the electrical conductivity (ECb). This study focuses on improving TDR measurements. The measured electrical conductivity is related to the geometry of the electromagnetic field which is a function of the probe design. The most important factors that define the field are the number, locations, and dimensions of the inner and outer conductors. An investigation of the influence of the probe head geometry on ECb is performed using groundwater modeling techniques. Finite difference and finite element models are created for different probe configurations used both in the field and in the laboratory and recommendations are made to correct the d.c. electrical conductivity measured in time domain reflectometry for different probe geometries. Temperature effects on Ka and ECb measurements in soils as well as the pulse area of the first and second reflections of the TDR waveform are also analyzed. Test results on compacted soils show that in cohesive soils the apparent dielectric constant increases with increasing temperatures, while it decreases with increasing temperature in non-cohesive soils. The electrical conductivity measurements are significantly more affected by temperature changes than the apparent dielectric constant; however, the trend appears to be relatively independent of soil type and texture. In both cohesive and non-cohesive soils, the electrical conductivity was observed to increases as the temperature increases. Both pulse areas decrease as the temperature increases;
Establishment of an antibody specific for cancer-associated haptoglobin: a possible implication of clinical investigation

We previously found that the serum level of fucosylated haptoglobin (Fuc-Hpt) was significantly increased in pancreatic cancer patients. To delineate the mechanism underlying this increase and develop a simple detection method, we set out to generate a monoclonal antibody (mAb) specific for Fuc-Hpt. After multiple screenings by enzyme-linked immunosorbent assay (ELISA), a 10-7G mAb was identified as being highly specific for Fuc-Hpt generated in a cell line as well as for Hpt derived from a pancreatic cancer patient. As a result from affinity chromatography with 10-7G mAb, followed by lectin blot and mass spectrometry analyses, it was found that 10-7G mAb predominantly recognized both Fuc-Hpt and prohaptoglobin (proHpt), which was also fucosylated. In immunohistochemical analyses, hepatocytes surrounding metastasized cancer cells were stained by the 10-7G mAb, but neither the original cancer cells themselves nor normal hepatocytes exhibited positive staining, suggesting that metastasized cancer cells promote Fuc-Hpt production in adjacent hepatocytes. Serum level of Fuc-Hpt determined with newly developed ELISA system using the 10-7G mAb, was increased in patients of pancreatic and colorectal cancer. Interestingly, dramatic increases in Fuc-Hpt levels were observed at the stage IV of colorectal cancer. These results indicate that the 10-7G mAb developed is a promising antibody which recognizes Fuc-Hpt and could be a useful diagnostic tool for detecting liver metastasis of cancer.
Berberine Protects Against Palmitate-Induced Apoptosis in Tubular Epithelial Cells by Promoting Fatty Acid Oxidation

Background Increased lipid accumulation in renal tubular epithelial cells (TECs) contributes to their injury and dysfunction and progression of tubulointerstitial fibrosis. Berberine (BBR), a natural plant alkaloid isolated from traditional medicine herbs, is effective in lowing serum lipid, and has a protective effect on chronic kidney disease (CKD) with dyslipidemia, including diabetic nephropathy. The aim of this study was to investigate the effect of BBR on palmitate (PA)-induced lipid accumulation and apoptosis in TECs. Material/Methods Human kidney proximal tubular epithelial cell line (HK-2) cells were treated with PA, BBR, and/or palmitoyltransferase 1A (CPT1A) inhibitor Etomoxir. Intracellular lipid content was assessed by Oil Red O and Nile Red staining. Cell apoptosis rate was evaluated by flow cytometry assay. The expression of apoptosis-related protein cleaved-caspase3 and fatty acid oxidation (FAO)-regulating proteins, including CPT1A, peroxisome proliferator-activated receptor α (PPARα), and PPARγ co-activator-1α (PGC1α), was measured by Western blot analysis and immunofluorescence. Results In the present study, PA treatment increased intracellular lipid deposition accompanied by elevated apoptosis in TECs compared with control group, whereas the protein expression of CPT1A, PPARα, and PGC1α, did not correspondingly increase in TECs. BBR significantly up-regulated the protein expression of CPT1A, PPARα, and PGC1α in TECs treated with or without PA, and reversed PA-induced intracellular lipid accumulation and apoptosis. Moreover, the CPT1A inhibitor Etomoxir counteracted the protective effect of BBR in TECs. Conclusions These in vitro findings suggest that PA can induce intracellular lipid accumulation and apoptosis in TECs, and the mechanism may be associated with inducing defective FAO, whereas BBR can protect TECs against PA-induced intracellular lipid accumulation and apoptosis by promoting FAO.
Multilocus Sequence Analysis of Nectar Pseudomonads Reveals High Genetic Diversity and Contrasting Recombination Patterns

The genetic and evolutionary relationships among floral nectar-dwelling Pseudomonas ‘sensu stricto’ isolates associated to South African and Mediterranean plants were investigated by multilocus sequence analysis (MLSA) of four core housekeeping genes (rrs, gyrB, rpoB and rpoD). A total of 35 different sequence types were found for the 38 nectar bacterial isolates characterised. Phylogenetic analyses resulted in the identification of three main clades [nectar groups (NGs) 1, 2 and 3] of nectar pseudomonads, which were closely related to five intrageneric groups: Pseudomonas oryzihabitans (NG 1); P. fluorescens, P. lutea and P. syringae (NG 2); and P. rhizosphaerae (NG 3). Linkage disequilibrium analysis pointed to a mostly clonal population structure, even when the analysis was restricted to isolates from the same floristic region or belonging to the same NG. Nevertheless, signatures of recombination were observed for NG 3, which exclusively included isolates retrieved from the floral nectar of insect-pollinated Mediterranean plants. In contrast, the other two NGs comprised both South African and Mediterranean isolates. Analyses relating diversification to floristic region and pollinator type revealed that there has been more unique evolution of the nectar pseudomonads within the Mediterranean region than would be expected by chance. This is the first work analysing the sequence of multiple loci to reveal geno- and ecotypes of nectar bacteria.
Reduced cognitive function, increased blood-brain-barrier transport and inflammatory responses, and altered brain metabolites in LDLr -/-and C57BL/6 mice fed a western diet

Recent work suggests that diet affects brain metabolism thereby impacting cognitive function. Our objective was to determine if a western diet altered brain metabolism, increased blood-brain barrier (BBB) transport and inflammation, and induced cognitive impairment in C57BL/6 (WT) mice and low-density lipoprotein receptor null (LDLr -/-) mice, a model of hyperlipidemia and cognitive decline. We show that a western diet and LDLr -/- moderately influence cognitive processes as assessed by Y-maze and radial arm water maze. Also, western diet significantly increased BBB transport, as well as microvessel factor VIII in LDLr -/- and microglia IBA1 staining in WT, both indicators of activation and neuroinflammation. Interestingly, LDLr -/- mice had a significant increase in 18F- fluorodeoxyglucose uptake irrespective of diet and brain 1H-magnetic resonance spectroscopy showed increased lactate and lipid moieties. Metabolic assessments of whole mouse brain by GC/MS and LC/MS/MS showed that a western diet altered brain TCA cycle and β-oxidation intermediates, levels of amino acids, and complex lipid levels and elevated proinflammatory lipid mediators. Our study reveals that the western diet has multiple impacts on brain metabolism, physiology, and altered cognitive function that likely manifest via multiple cellular pathways.
General Patterns of Diversity in Major Marine Microeukaryote Lineages

Microeukaryotes have vital roles for the functioning of marine ecosystems, but still some general characteristics of their current diversity and phylogeny remain unclear. Here we investigated both aspects in major oceanic microeukaryote lineages using 18S rDNA (V4–V5 hypervariable regions) sequences from public databases that derive from various marine environmental surveys. A very carefully and manually curated dataset of 8291 Sanger sequences was generated and subsequently split into 65 taxonomic groups (roughly to Class level based on KeyDNATools) prior to downstream analyses. First, we calculated genetic distances and clustered sequences into Operational Taxonomic Units (OTUs) using different distance cut-off levels. We found that most taxonomic groups had a maximum pairwise genetic distance of 0.25. Second, we used phylogenetic trees to study general evolutionary patterns. These trees confirmed our taxonomic classification and served to run Lineage Through Time (LTT) plots. LTT results indicated different cladogenesis dynamics across groups, with some displaying an early diversification and others a more recent one. Overall, our study provides an improved description of the microeukaryote diversity in the oceans in terms of genetic differentiation within groups as well as in the general phylogenetic structure. These results will be important to interpret the large amount of sequence data that is currently generated by High Throughput Sequencing technologies.
Effect of projectile nose geometry on the critical velocity and failure of yarn subjected to transverse impact

Three different types of yarn have been subjected to transverse impact experiments in efforts to gain an understanding of local yarn failure and to provide input parameters for future transverse yarn impact simulations. Dupont™ Kevlar® KM2, DSM Dyneema® SK76, and AuTx® from JSC Kamenskvolokno were selected as representative materials, as the former two are commonly implemented into bullet resistant panels and the latter is a promising material for future impact resistant fabrics. In order to assess the effect of projectile nose shape on the critical rupture velocity range for each yarn type, three missile geometries have been implemented, namely a 0.30 caliber rounded head, a 0.30 caliber chisel nosed fragment simulation projectile (FSP), and a high-carbon steel razor blade. As opposed to one single velocity wherein yarn behavior transitions from transverse wave development to immediate local failure, a range is defined wherein progressive filament failure is detected with increasing impact velocities. Such ranges are determined for all yarn types using the three projectile geometries yielding critical velocity transition regions of increasing value when impacting via razor blade, FSP, and round projectile heads, accordingly. In addition, post-mortem fracture surfaces recovered from impact experiments have been imaged so as to elucidate the mechanism of failure throughout the range of velocities tested for each projectile type and yarn material and said fracture surfaces correlate well with impact velocity and projectile nose geometry.
Whole Blood Cytokine Response to Local Traffic-Related Particulate Matter in Peruvian Children With and Without Asthma

This study sought to investigate if acute phase immune responses of whole blood from Peruvian children with controlled and uncontrolled asthma differed from children without asthma, following exposure to traffic-related particulate matter (TRPM). TRPM, including particulate matter from diesel combustion, has been shown to stimulate acute airway inflammation in individuals with and without asthma. For this study, a whole blood assay (WBA) was used to test peripheral whole blood samples from 27 children with asthma, and 12 without asthma. Participant blood samples were stimulated, ex vivo, for 24-h with an aqueous extract of TRPM that was collected near study area highways in Lima, Peru. All participant blood samples were tested against the same TRPM extract, in addition to purified bacterial endotoxin and pyrogen-free water, which served as positive and negative WBA controls, respectively. The innate and adaptive cytokine responses were evaluated in cell-free supernatants of the whole blood incubations. Comparatively similar levels were recorded for nine out of the 10 cytokines measured [e.g., – Interleukin (IL)-1β, IL-6, IL-10], regardless of study participant asthma status. However, IL-8 levels in TRPM-stimulated blood from children with uncontrolled asthma were diminished, compared to subjects without asthma (633 pg/ml vs. 1,023 pg/ml, respectively; p < 0.01); IL-8 responses for subjects with controlled asthma were also reduced, but to a lesser degree (799 pg/ml vs. 1,023 pg/ml, respectively; p = 0.10). These relationships were present before, and after, adjusting for age, sex, obesity/overweight status, C-reactive protein levels, and residential proximity to the study area’s major roadway. For tests conducted with endotoxin, there were no discernible differences in cytokine response between groups, for all cytokines measured. The WBA testing conducted for this study highlighted the capacity of the TRPM extract to potently elicit the release of IL-8 from the human whole blood system. Although the small sample size of the study limits the capacity to draw definitive conclusions, the IL-8 responses suggest that that asthma control may be associated with the regulation of a key mediator in neutrophil chemotaxis, at a systemic level, following exposure to PM derived from traffic-related sources.
Towards Generalization and Simplicity in Continuous Control

This work shows that policies with simple linear and RBF parameterizations can be trained to solve a variety of continuous control tasks, including the OpenAI gym benchmarks. The performance of these trained policies are competitive with state of the art results, obtained with more elaborate parameterizations such as fully connected neural networks. Furthermore, existing training and testing scenarios are shown to be very limited and prone to over-fitting, thus giving rise to only trajectory-centric policies. Training with a diverse initial state distribution is shown to produce more global policies with better generalization. This allows for interactive control scenarios where the system recovers from large on-line perturbations; as shown in the supplementary video.
Solid lipid nanoparticles as a vehicle for brain-targeted drug delivery : two new strategies of functionalization with apolipoprotein

Nanotechnology can be an important tool to improve the permeability of some drugs for the blood–brain barrier. In this work we created a new system to enter the brain by functionalizing solid lipid nanoparticles with apolipoprotein E, aiming to enhance their binding to low-density lipoprotein receptors on the blood–brain barrier endothelial cells. Solid lipid nanoparticles were successfully functionalized with apolipoprotein E using two distinct strategies that took advantage of the strong interaction between biotin and avidin. Transmission electron microscopy images revealed spherical nanoparticles, and dynamic light scattering gave a Z-average under 200 nm, a polydispersity index below 0.2, and a zeta potential between −10 mV and −15 mV. The functionalization of solid lipid nanoparticles with apolipoprotein E was demonstrated by infrared spectroscopy and fluorimetric assays. In vitro cytotoxic effects were evaluated by MTT and LDH assays in the human cerebral microvascular endothelial cells (hCMEC/D3) cell line, a human blood–brain barrier model, and revealed no toxicity up to 1.5 mg ml over 4 h of incubation. The brain permeability was evaluated in transwell devices with hCMEC/D3 monolayers, and a 1.5-fold increment in barrier transit was verified for functionalized nanoparticles when compared with non-functionalized ones. The results suggested that these novel apolipoprotein E-functionalized nanoparticles resulted in dynamic stable systems capable of being used for an improved and specialized brain delivery of drugs through the blood–brain barrier. S Online supplementary data available from stacks.iop. org/NANO/26/495103/mmedia Nanotechnology Nanotechnology 26 (2015) 495103 (11pp) doi:10.1088/0957-4484/26/49/495103 5 These authors have contributed equally to this work. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. 0957-4484/15/495103+11$33.00 © 2015 IOP Publishing Ltd Printed in the UK 1
Surface Potential Decay of Low Density Polyethylene (LDPE) Films under Different Corona Discharge Conditions

The surface potential decay on low-density polyethylene (LDPE) has been studied after negative charge deposition by corona effect as a function of initial potential Vo and charging time tc. It was found that the decay rate increases with initial potential and charging time. For high charging level, cross over phenomenon has been observed. Surface potential profile has been recorded with two different charging levels. The surface potential profile indicates the absence of lateral migration of charges along the free surface of the LDPE film and the presence of the hollow for high initial potential, which makes the bulk conduction mechanisms caused by charge injection into the LDPE film bulk, responsible for the observed surface potential decay.
Key differences between lakes and reservoirs modify climate signals: A case for a new conceptual model

Lakes and reservoirs are recognized as important sentinels of climate change, integrating catchment and atmospheric climate change drivers. Climate change conceptual models generally consider lakes and reservoirs together despite the possibility that these systems respond differently to climate‐related drivers. Here, we synthesize differences between lake and reservoir characteristics that are likely important for predicting waterbody response to climate change. To better articulate these differences, we revised the energy mass flux framework, a conceptual model for the effects of climate change on lentic ecosystems, to explicitly consider the differential responses of lake versus reservoir ecosystems. The model predicts that catchment and management characteristics will be more important mediators of climate effects in reservoirs than in natural lakes. Given the increased reliance on reservoirs globally, we highlight current gaps in our understanding of these systems and suggest research directions to further characterize regional and continental differences among lakes and reservoirs.
The α6β4 integrin promotes resistance to ferroptosis

Increases in lipid peroxidation can cause ferroptosis, a form of cell death triggered by inhibition of glutathione peroxidase 4 (GPX4), which catalyzes the reduction of lipid peroxides and is a target of ferroptosis inducers, such as erastin. The &agr;6&bgr;4 integrin protects adherent epithelial and carcinoma cells from ferroptosis induced by erastin. In addition, extracellular matrix (ECM) detachment is a physiologic trigger of ferroptosis, which is evaded by &agr;6&bgr;4. The mechanism that enables &agr;6&bgr;4 to evade ferroptosis involves its ability to protect changes in membrane lipids that are proferroptotic. Specifically, &agr;6&bgr;4-mediated activation of Src and STAT3 suppresses expression of ACSL4, an enzyme that enriches membranes with long polyunsaturated fatty acids and is required for ferroptosis. Adherent cells lacking &agr;6&bgr;4 require an inducer, such as erastin, to undergo ferroptosis because they sustain GPX4 expression, despite their increase in ACSL4. In contrast, ECM detachment of cells lacking &agr;6&bgr;4 is sufficient to trigger ferroptosis because GPX4 is suppressed. This causal link between &agr;6&bgr;4 and ferroptosis has implications for cancer biology and therapy.