Miracle-dz/UniFAR-FTUs huggingface dataset:
Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-β Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation

Cutaneous beta-papillomaviruses are associated with non-melanoma skin cancers that arise in patients who suffer from a rare genetic disorder, Epidermodysplasia verruciformis (EV) or after immunosuppression following organ transplantation. Recent studies have shown that the E6 proteins of the cancer associated beta human papillomavirus (HPV) 5 and HPV8 inhibit NOTCH and TGF-β signaling. However, it is unclear whether disruption of these pathways may contribute to cutaneous HPV pathogenesis and carcinogenesis. A recently identified papillomavirus, MmuPV1, infects laboratory mouse strains and causes cutaneous skin warts that can progress to squamous cell carcinoma. To determine whether MmuPV1 may be an appropriate model to mechanistically dissect the molecular contributions of cutaneous HPV infections to skin carcinogenesis, we investigated whether MmuPV1 E6 shares biological and biochemical activities with HPV8 E6. We report that the HPV8 and MmuPV1 E6 proteins share the ability to bind to the MAML1 and SMAD2/SMAD3 transcriptional cofactors of NOTCH and TGF-beta signaling, respectively. Moreover, we demonstrate that these cutaneous papillomavirus E6 proteins inhibit these two tumor suppressor pathways and that this ability is linked to delayed differentiation and sustained proliferation of differentiating keratinocytes. Furthermore, we demonstrate that the ability of MmuPV1 E6 to bind MAML1 is necessary for papilloma formation in experimentally infected mice. Our results, therefore, suggest that experimental MmuPV1 infection in mice will be a robust and useful experimental system to model key aspects of cutaneous HPV infection, pathogenesis and carcinogenesis.
THE EFFECTS OF REFLECTIVE PRACTICE ON TEACHER PERCEPTIONS OF WORK RELATED STRESSORS FOR EARLY CHILDHOOD EDUCATORS

OF THE THESIS The Effects of Reflective Practice on Teacher Perceptions of Work Related Stressors for Early Childhood Educators by Elizabeth Kay Diaz Master of Science in Child Development San Diego State University, 2012 Teaching in an early child care environment is highly stressful with many demands, such as challenging child behaviors, paperwork, and administrative demands. A demanding work environment can be linked to a decrease in job satisfaction and consequently teacher turnover. Compared to other teacher settings, teacher turnover rates in early child care centers are greater. The negative impact teacher turnover has on the children they serve, in addition to the overall psychological and physical health of the teachers is why it is imperative for early child care teachers to be supported in gaining skills to increase their sensitivity and coping strategies. This study examines the use of reflective practice for early childhood educators and whether it leads to a positive effect on teacher perceptions of work related stressors. The participants were sixteen lead teachers from the three of the center’s site locations. Quantitative data were used to gain an understanding of teacher perceptions of work related resources and demands, psychological coping strategies, and their ability to selfregulate and use insight. Qualitative data were collected from journals that provided insight in to teacher’s reflections on each one of the eight collaborative group sessions. From the triangulation of the data many stress-related themes were discovered which include, lack of administrative support, unmanageable workload, and challenging child behaviors. The findings from this study indicate that reflective practice may have a positive impact on teachers’ perceptions of stress. A replication of the study with an increase in the length of the treatment as well as an increase in the sample size would allow for greater generalizability. The findings also suggest that these early childhood educators are experiencing unmanageable amounts of stress which has been linked to teacher turnover.
Emerging Genotypes of Human Respiratory Syncytial Virus Subgroup A among Patients in Japan

ABSTRACT Human respiratory syncytial virus (HRSV) is a common etiological agent of acute lower respiratory tract disease in infants. We report the molecular epidemiology of HRSV in Niigata, Japan, over six successive seasons (from 2001 to 2007) and the emerging genotypes of HRSV subgroup A (HRSV-A) strains. A total of 488 HRSV samples were obtained from 1,103 screened cases in a pediatric clinic in Niigata. According to the phylogenetic analysis, among the PCR-positive samples, 338 HRSV-A strains clustered into the previously reported genotypes GA5 and GA7 and two novel genotypes, NA1 and NA2, which were genetically close to GA2 strains. One hundred fifty HRSV-B strains clustered into three genotypes, namely, GB3, SAB3, and BA, which has a 60-nucleotide insertion in the second hypervariable region of the G protein. The NA1 strains emerged first, in the 2004-2005 season, and subsequently, the NA2 strain emerged in the 2005-2006 season. Both strains caused large epidemics in the 2005-2006 and 2006-2007 seasons. The average age of children who were infected with NA2 strains was significantly higher than that of those infected with GA5 and the frequency of reinfection by NA2 was the highest among all genotypes, suggesting that this genotype possessed new antigenicity for evading past host immunity. This is the first paper to show a possible correlation between an emerging genotype, NA2, and large outbreaks of HRSV in Japan. Continuing studies to follow up the genetic changes and to clarify the mechanism of reinfection in HRSV are important steps to understand HRSV infections.
Steps toward Peace in Civil Wars in the Asia-Pacific Region: A Disaggregate Approach to Civil War Settlements

Regardless of the types of civil conflict settlements, all parties generally enter into some sorts of tacit or direct bargaining in the course of civil conflict, namely, in steps toward peace. In contrast to a basically static framework employed in much of existing literature on civil war settlements and mediation, this article proposes a disaggregate approach to dynamic and multi-phase processes in civil conflict termination via negotiations. We illustrate a conceptual and theoretical framework to examine four steps in civil conflict settlements in a large-N research program. In so doing, we present an initial effort to construct a dyadic dataset isolating processes that allow civil conflict settlements to progress or regress between low and higher levels of agreement in the Asia-Pacific region from 1990 to 2005. We discuss a set of preliminary simple statistical results for the four distinct settlement phases in the context of conflict and rebel characteristics. Among the findings of note, third parties provide important assistance in nurturing successful negotiations especially in the context of waning insurgent strength. Peace proposals originate most frequently from governments, and seem to hinge especially on opponents’ battlefield advantages. Evidence of mutually hurting stalemates is also found. In the article’s conclusion we elaborate a long-term research agenda.
Police Use Of Force And Suspect Resistance: The Micro Process Of The Police-Suspect Encounter

Using data collected as part of an observational study of the police in Indianapolis, Indiana, and St. Petersburg, Florida, this research examines 3,544 police-suspect encounters in an attempt to better understand the application of nonlethal force and the relationship between officer use of force and suspect resistance. More specifically, it examines the extent of and variation in force and resistance and the interplay between the two. Results show that when both verbal and physical forms of coercion are considered, force occurs quite frequently—in more than half of all encounters. Conversely, suspects displayed some form of resistance in 12% of all encounters. When multiple uses of force and resistance within individual encounters are considered, the frequency of both behaviors increases substantially. In addition, a majority of both forceful and resistant behaviors fall on the lower end of continuum (e.g., verbal commands as opposed to striking with a baton). Encounters that began with some form of force resulted in a greater frequency of subsequent suspect resistance and an increased use of additional force at some later point in the encounters—calling into question the utility of a “take charge” approach to maintaining control within police-suspect encounters.
Infection studies on human cell lines with porcine circovirus type 1 and porcine circovirus type 2

Abstract: Background: The lack of human donor organs in allotransplantation has led to a proposal for the use of porcine tissues and organs as alternative therapeutic material for humans. Besides immunological problems like graft rejection, one of the major concerns is the transmission of porcine microorganisms as viruses, bacteria and fungi to a human recipient.
The gender difference in distance running performance has plateaued: an analysis of world rankings from 1980 to 1996.

PURPOSE Annual world rankings from 1980-1996 were used i) to determine if the gender difference in distance running events has stabilized, and ii) to examine whether the relative decline in pace as race distance increases is different between men and women. World best (WB) and 100th best times in the 1500 m and the marathon (42 km) for each of the 17 years were the indices used to represent overall trends in men's vis-à-vis women's distance running. METHODS These data were analyzed using regression analyses to develop and compare gender-specific equations. In the 1500 m, the gender difference in WB times (11.1 +/- 1.1%) was consistent from 1980 to 1996, and the slight rate of improvement in event depth (i.e., 100th rankings) was similar for men and women. RESULTS In the marathon, the gender difference in WB times (11.2 +/- 0.9%) was essentially the same as for the 1500 m. In 1980, the marathon was a fairly new event for the women, having only just been sanctioned by the International Amateur Athletics Federation (IAAF). As a consequence, the depth of the field increased quickly from 1980 to 1984. Since the mid-1980s, the rate of improvement for women in 100th-ranked times has leveled off to equal that of men. The average declines in relative pace for men and women from the 1500 m to the 10 km to the marathon were found to be remarkably similar with no diminishing of the gender difference as race distance increased. CONCLUSIONS Based on worldwide indices of competitive distance running, the gender difference in distance running performance has plateaued in recent years. Concomitantly, over the past decade, opportunities for women to train and compete have approached parity with those of men. It is likely that the current gender difference in performance will remain fairly constant because of biological differences between men and women that give men an advantage in distance running.
Gastric Glomus Tumor: A Rare Cause of Upper Gastrointestinal Bleeding

A 24-year-old woman was referred to our department because of melena. These symptoms combined with severe anemia prompted us to perform an emergency upper endoscopy, which showed bleeding from an ulcerated 30 mm submucosal tumor in the gastric antrum. A computed tomography scan revealed a homogeneously enhanced mass, and endoscopic ultrasonography identified a well-demarcated mass in the third and fourth layers of the gastric wall. Because analysis of the possible medical causes remained inconclusive and the risk of rebleeding, laparoscopy-assisted gastric wedge resection was performed after administration of 10 units of red cell concentrate. Histological and immunohistological analysis revealed the tumor to be a gastric glomus tumor. Gastric submucosal tumors remain challenging to diagnose preoperatively as they show a variety of radiologic and clinicopathologic features and are associated with the risk of bleeding upon biopsy, as is indicated in the guidelines for gastric submucosal tumors. Gastric glomus tumors characteristically present with exsanguinating gastrointestinal hemorrhaging that often requires blood transfusion. Additionally, gastric submucosal tumors typically occur in elderly patients; however, this case involved a young patient who was 24 years old. Here, we describe this case in order to identify features that may aid in early differentiation of gastric submucosal tumors.
The Responsiveness Problem in Psychotherapy: A Review of Proposed Solutions

Therapist responsiveness is defined as therapist behavior being influenced by emerging context. Responsiveness is ubiquitous and creates serious problems for a ballistic, cause–effect understanding of how psychotherapy works. This conceptual literature review examines ways psychotherapy researchers have constructively engaged the responsiveness problem. We note classical approaches to the responsiveness problem and review all available citations of the formulation of the problem by Stiles, Honos-Webb, and Surko (1998, p. 439), focusing on proposed solutions. We identified N = 58 studies that cited the 1998 article and engaged with the responsiveness problem. These along with additional engagements with the responsiveness problem identified by us and by colleagues were reviewed. We distinguished six categories of ways researchers have addressed the responsiveness problem: (a) demonstrating effects of responsiveness, (b) measuring responsiveness quantitatively, (c) describing responsiveness qualitatively, (d) using evaluative measures, (e) developing responsive clinical interventions, and (f) extending responsiveness concepts to related domains. There are ways to engage the responsiveness problem that are scientifically productive. However, appropriately engaging the problem may require some psychotherapy researchers to ask different questions than they have previously asked.
Detection of Infectious Laryngotracheitis Virus in Formalin-Fixed, Paraffin-Embedded Tissues by Nested Polymerase Chain Reaction

SUMMARY. Infectious laryngotracheitis virus (ILTV) is routinely diagnosed by histopathologic examination of trachea, eyelid, and lung tissues. Lesions consistent with infectious laryngotracheitis (ILT) infection include syncytial cell formation with intranuclear inclusion bodies. These changes are present during the acute phase of infection. To increase the sensitivity of detecting ILT, a nested polymerase chain reaction (PCR) was developed for detection of ILTV DNA. Nested PCR assay was specific for the amplification of ILTV DNA and did not amplify a variety of other avian pathogens. To further validate the ability of this assay to detect ILT, nested PCR was performed in formalin-fixed, paraffin-embedded tissues from 35 cases of respiratory disease. Of the 35 cases, 12 were considered ILT suspects on the basis of initial clinical observation. Eleven of the 12 ILT-suspect cases were diagnosed as ILT, and the remaining 24 were diagnosed as nonspecific tracheitis (NST) by histopathologic examination. Histopathologically positive samples were confirmed by direct fluorescent antibody test and virus isolation. Of the 11 ILT-positive cases, 10 were positive by nested PCR. In addition, ILTV DNA was detected in 7 of the 24 samples diagnosed as NST upon histopathologic examination. Therefore, by nested PCR, ILTV DNA was detected in tissues independently of the presence of syncytial cells, intranuclear inclusions, or both. ILT nested PCR is a specific and sensitive assay capable of detecting ILT at different stages of infection and can be utilized in combination with histopathological examination to accelerate the diagnosis of ILT infection.
Consumption of fructose and high fructose corn syrup increase postprandial triglycerides, LDL-cholesterol, and apolipoprotein-B in young men and women.

CONTEXT The American Heart Association Nutrition Committee recommends women and men consume no more than 100 and 150 kcal of added sugar per day, respectively, whereas the Dietary Guidelines for Americans, 2010, suggests a maximal added sugar intake of 25% or less of total energy. OBJECTIVE To address this discrepancy, we compared the effects of consuming glucose, fructose, or high-fructose corn syrup (HFCS) at 25% of energy requirements (E) on risk factors for cardiovascular disease. PARTICIPANTS, DESIGN AND SETTING, AND INTERVENTION: Forty-eight adults (aged 18-40 yr; body mass index 18-35 kg/m(2)) resided at the Clinical Research Center for 3.5 d of baseline testing while consuming energy-balanced diets containing 55% E complex carbohydrate. For 12 outpatient days, they consumed usual ad libitum diets along with three servings per day of glucose, fructose, or HFCS-sweetened beverages (n = 16/group), which provided 25% E requirements. Subjects then consumed energy-balanced diets containing 25% E sugar-sweetened beverages/30% E complex carbohydrate during 3.5 d of inpatient intervention testing. MAIN OUTCOME MEASURES Twenty-four-hour triglyceride area under the curve, fasting plasma low-density lipoprotein (LDL), and apolipoprotein B (apoB) concentrations were measured. RESULTS Twenty-four-hour triglyceride area under the curve was increased compared with baseline during consumption of fructose (+4.7 ± 1.2 mmol/liter × 24 h, P = 0.0032) and HFCS (+1.8 ± 1.4 mmol/liter × 24 h, P = 0.035) but not glucose (-1.9 ± 0.9 mmol/liter × 24 h, P = 0.14). Fasting LDL and apoB concentrations were increased during consumption of fructose (LDL: +0.29 ± 0.082 mmol/liter, P = 0.0023; apoB: +0.093 ± 0.022 g/liter, P = 0.0005) and HFCS (LDL: +0.42 ± 0.11 mmol/liter, P < 0.0001; apoB: +0.12 ± 0.031 g/liter, P < 0.0001) but not glucose (LDL: +0.012 ± 0.071 mmol/liter, P = 0.86; apoB: +0.0097 ± 0.019 g/liter, P = 0.90). CONCLUSIONS Consumption of HFCS-sweetened beverages for 2 wk at 25% E increased risk factors for cardiovascular disease comparably with fructose and more than glucose in young adults.
Toll-like receptor 2 induces Th17 myeloperoxidase autoimmunity while Toll-like receptor 9 drives Th1 autoimmunity in murine vasculitis.

OBJECTIVE Autoantibodies constitute the hallmark of antineutrophil cytoplasmic antibody-associated vasculitis (AAV); however, CD4+ T cells play an essential role in the development of autoimmunity. Infection is associated with vasculitis, with Toll-like receptors (TLRs) a potential link between infection and autoimmunity. This study was undertaken to investigate the role of TLR ligation on cellular and humoral autoimmunity and glomerular injury in experimental myeloperoxidase (MPO)-induced AAV. METHODS We analyzed autoimmune responses in wild-type mice immunized with MPO alone or coimmunized with MPO and a TLR-2 or TLR-9 ligand. The major vascular injury found in human disease, glomerulonephritis with focal necrosis, was triggered by administering a subnephritogenic dose of nephrotoxic serum. RESULTS MPO alone induced low-titer antineutrophil cytoplasmic antibodies (ANCAs) without delayed-type hypersensitivity or CD4 cytokine responses. However, when MPO was given with either TLR ligand, cellular and humoral autoimmunity was enhanced, but with distinctly different CD4 subsets and IgG ANCA isotypes. TLR-2 ligand induced Th17 autoimmunity, with retinoic acid receptor-related orphan nuclear receptor γt-dependent interleukin-17A (IL-17A) production. TLR-9 ligand promoted Th1 autoimmunity, with enhanced production of interferon-γ (IFNγ) and Th1-associated IgG subclasses. Glomerular vasculitis developed only after the administration of nephrotoxic serum in mice immunized with either TLR ligand and MPO. Glomerulonephritis directed by MPO and TLR-2 ligation was attenuated when IL-17A was neutralized, while glomerulonephritis induced by MPO and TLR-9 ligation was attenuated when IFNγ was neutralized. CONCLUSION Our findings indicate a pathogenic role of TLRs in initiating autoimmune AAV. TLR-2 induces Th17 CD4 cells while TLR-9 can also direct vasculitis, by directing Th1 autoimmunity.
Clinical Characteristics of Stenotrophomonas maltophilia Bacteremia: A Regional Report and a Review of a Japanese Case Series

Objective Stenotrophomonas maltophilia is an emerging nosocomial pathogen that causes fatal infections in critically ill or immunocompromised patients. S. maltophilia bacteremia (SMB) is a rare condition, and its clinical characteristics in Japanese settings are not well known. Methods The medical charts of patients with SMB were retrospectively reviewed at two medical facilities (Okayama University Hospital and Tsuyama Chuo Hospital) for seven years. The data were analyzed along with those previously reported from other Japanese facilities. Result A total of 181 patients (110 men and 71 women) were evaluated. The major underlying diseases included hematologic malignancy (36.5%), solid organ malignancy (25.4%), and neutropenia (31.5%). The recent use of carbapenem was seen in 56.9% of the cases in total, and more than one-third of the patients in our hospitals were treated with carbapenem at the onset of SMB. Of 28 (63.6%) of 44 cases treated for S. maltophilia, those who did not survive were more likely to have been treated with broad-spectrum antibiotics. A multivariate analysis revealed that a higher updated Charlson Comorbidity Index [odds ratio (95% confidence interval), 1.75 (1.11-2.75); p=0.015] and intubation [odds ratio (95% confidence interval), 12.6 (1.62-97.9); p=0.016] were associated with mortality in our cases. Pathogens were often resistant to ceftazidime but susceptible to minocycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. The overall mortality rates within 30 and 90 days were 37.5% and 62.5%, respectively. Conclusion The clinical characteristics of SMB in Japanese cases were similar to those reported from other countries. Clinicians should be aware that breakthrough infection by S. maltophilia may occur during administration of carbapenem.
The New Cultural Conflict, Polarization, and Representation in the Swiss Party System, 1975–2011

Party relationships in the Swiss party system have become substantially more antagonistic over the past decades. This article analyzes the actors and ideologies that have triggered the emergence of a polarizing cultural antagonism, and shows that this conflict is not primarily about Switzerland’s relationship with Europe. The implications of the emergence of the new cultural conflict for democracy are far from clear. Using an innovative approach to measure the congruence between voter preferences and party positions, I show that the representation of citizens’ interests along the cultural dimension has improved significantly since the 1970s. The concomitant polarization of the economic dimension has not altered the quality of representation, on the other hand. I conclude by discussing the trade-offs between the political articulation of a traditionalist-communitarian conception of community that clearly resonates with an important part of the Swiss populace, and other components of liberal democracy.
Optimum Bias Load-Line Compensates Temperature Variation of Junction Diode's RF Resistance

This paper presents a novel temperature-compensation technique to compensate variation of the junction diode's RF resistance. Here we have theoretically analyzed and experimentally demonstrated the temperature sensitivity of the junction diode's RF resistance and proposed an optimum dc-bias load line technique to minimize temperature variation of RF resistance of the Schottky barrier, p-i-n, and p-n junction diodes. The proposed optimum load line biasing technique eliminates the requirement of conventional temperature-compensation circuits with temperature sensors to achieve temperature-invariant RF performance of diode-based RF circuits such as a linearizer, attenuator, phase shifter, etc. for various RF applications where it experiences wide temperature variations. The circuit responds directly to the junction temperature of the diode; thus, there will be no compensation error due to temperature gradient and self-heating of the diodes. This technique is very simple, accurate, and suitable to implement in monolithic-microwave integrated-circuit technology