Miracle-dz/UniFAR-FTUs huggingface dataset:
Research on Key Technologies of Microarray Chips for Detecting Drug-Resistant Genes in Helicobacter pylori

In addressing the detection of drug resistance in Helicobacter pylori, we have successfully developed an efficient and highly accurate detection methodology. Initially, we designed and fabricated a microarray chip, which underwent finite element analysis for its optical and thermal characteristics. Ultimately, COC material was chosen as the processing material for the chip, ensuring superior performance. Subsequently, we established a comprehensive detection system and validated its performance. Following that, comparative experiments were conducted for detecting drug resistance in H. pylori. The experimental results indicate that our established methodology aligns with the results obtained using the E-test detection kit, achieving a concordance rate of 100%. In comparison to the E-test detection kit, our methodology reduces the detection time to 1.5 h and provides a more extensive coverage of detection sites.
Associations of gender and a proxy of female menopausal status with histological features of drug‐induced liver injury

Gender and menopause may contribute to type and severity of drug‐induced liver injury (DILI) by influencing host responses to injury. The aim of this study was to assess the associations of gender and female age 50 [a proxy of menopause] with histological features of liver injury in 212 adults enrolled in the Drug‐Induced Liver Injury Network (DILIN) registry.
Activation of chloride current by P2‐purinoceptors in rat ventricular myocytes

1 Rat ventricular myocytes were dissociated and their responses to extracellularly applied ATP were recorded using patch pipettes under the whole cell configuration. 2 ATP initially induced an inward current followed by an outward current at − 50 mV. With a Cs‐rich pipette solution the late outward current was blocked, leaving a sustained inward current (IATPs) suggesting that a K+conductance underlies the late response. 3 When the extracellular Cl−concentration was changed, the reversal potential of IATPs corresponded well to the shift of the Cl−equilibrium potential. IATPs was reversibly blocked by the chloride channel blocker, 4,4′‐diisothiocyanatostilbene‐2,2′‐disulphonic acid (DIDS). 4 The concentration‐response curve of IATPs had a Hill coefficient of 0.98 and an EC50 value of 5.2 × 10−6m. 5 ATP was more potent than ADP, while AMP and adenosine were ineffective, suggesting that P2‐purinoceptor activation induced IATPs. 6 The activation of IATPs was depressed by depleting the extracellular Mg2+and increased by adding Mg2+. 7 Our results strongly suggest that P2‐purinoceptor activation by ATP induces both a Cl−‐conductance (IATPs) and a K+‐conductance in rat ventricular myocytes.
The Effect of the Theo Van Gogh Murder on House Prices in Amsterdam

Does Islamic terrorism have an effect on the general attitudes towards Muslim minorities? We use the murder of Theo van Gogh as an event study to address this question. Specifically, we use the hedonic-market model and test for an effect on listed house prices in neighbourhoods where more than 25% of the people belong to an ethnic minority from a Muslim country (type I). Relative to the other neighbourhoods, house prices in type I neighbourhoods decreased in 10 months by about 3%, with a widening gap over time. We use a unique weekly data set from a multi listing service that contains over 70% of all houses for sale and obtain the actual transaction prices from the registers office. Our results are robust to several adjustments including changes in the control group. There is no significant difference in the time it takes for houses to be sold in type I versus other neighbourhoods. Finally, we find evidence that segregation increased. People belonging to the Muslim minority were more likely to buy and less likely to sell a house in a type I neighbourhood after the murder than before.
COLORS AND KINEMATICS OF L DWARFS FROM THE SLOAN DIGITAL SKY SURVEY

We present a sample of 484 L dwarfs, 210 of which are newly discovered from the Sloan Digital Sky Survey (SDSS) Data Release 7 spectroscopic database. We combine this sample with known L dwarfs to investigate their izJHKS colors. Our spectroscopically selected sample has ∼0.1 mag bluer median J − KS color at a given spectral type (for L0–L4) than previously known L dwarfs, which reflects a bias toward redder L dwarfs in past selection criteria. We present photometric distance relations based on i − z and i − J colors and derive distances to our L dwarf sample. We combine the distances with SDSS/2MASS proper motions in order to examine the tangential velocities. For the majority of our spectroscopic sample, we measured radial velocities and present three-dimensional kinematics. We also provide Hα detections for the fraction of our sample with sufficient quality spectra. Comparison of the velocities of our L dwarf sample to a kinematic model shows evidence for both cold and hot dynamical populations, consistent with young and old disk components. The dispersions of these components are similar to those found for M dwarfs. We also show that J − KS color is correlated with velocity dispersion, confirming a relationship between J − KS color and age.
Impact of physical activity on postural stability in pregnant and postpartum women

Purpose. regular physical activity is recommended by the WHO for women in the perinatal period as one of the basic factors determining the physiological course of pregnancy, childbirth and the postpartum period. the study aimed to identify the relationship between regular physical training of pregnant women, carried out in the second and third trimesters of pregnancy, and postural stability during the perinatal period. Methods. 58 women in the first trimester of pregnancy took part in the study and were divided into two groups according to their own choice. the exercise group participated in physical training three times a week during the second and third trimesters of pregnancy. the non-exercise group did not engage in additional physical activity. All subjects underwent three postural stability tests using a stabilograph. Results. In assessing posture stability parameters, significant differences were found only in body coordination in the active group of women. the exercise group showed statistically significantly lower values of the stabilogram parameters (better postural stability) obtained postpartum compared to those taken during pregnancy. there were no significant differences between the results taken during pregnancy. In the group of non-exercising women, no significant differences were observed between the second measurement during pregnancy and the measurement made postpartum. Conclusions. It has been shown that physical activity has a significant impact on improving the coordination of women during pregnancy and postpartum. Women participating in regular physical training were characterised by smaller stabilogram deflections and better postural control compared to the control group.
Expression of Viral MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma

ABSTRACT Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs). We found that BART miRNAs but not BHRF1 miRNAs were expressed in EBV-infected gastric carcinoma cell lines and the tumor tissues from patients as well as the animal model. The expression of viral miRNAs in EBVaGCs suggests that these EBV miRNAs may play important roles in the tumorigenesis of EBVaGCs.
The Acanthamoeba spp. in Water Sources from Zanjan Province, Northwest of Iran

Background: The genus of Acanthamoeba is an opportunistic protozoan parasite with a worldwide distribution where it is able to cause fatal granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK). This organism inhabits in the wide range of natural and man-made aquatic environments. The present study was carried out to evaluate the presence of Acanthamoeba spp. in the various water resources of Zanjan province, northwest Iran, using both morphological and molecular methods. Methods: The Water samples were randomly collected from 30 water sources in different parts of Zanjan, Iran, between April 2015 and May 2016. Then, the samples were cultured on non-nutrient agar and the Acanthamoeba genus identified by morphological characters. The polymerase chain reaction (PCR) was performed using the 18S rRNA gene as a molecular marker. Results: The obtained data showed that, out of the 60 water samples collected, 30 (50%) were for Acanthamoeba spp. According to morphological and molecular approaches. investigation
A Rising Cancer Prevention Target of RSK2 in Human Skin Cancer

RSK2 is a p90 ribosomal S6 kinase family (p90RSK) member regulating cell proliferation and transformation induced by tumor promoters such as epithelial growth factor (EGF) and 12-O-tetradecanoylphorbol-13-acetate. This family of p90RSK has classified as a serine/threonine kinase that respond to many growth factors, peptide hormones, neurotransmitters, and environmental stresses such as ultraviolet (UV) light. Our recent study demonstrates that RSK2 plays a key role in human skin cancer development. Activation of RSK2 by EGF and UV through extracellular-activated protein kinases signaling pathway induces cell cycle progression, cell proliferation, and anchorage-independent cell transformation. Moreover, knockdown of RSK2 by si-RNA or sh-RNA abrogates cell proliferation and cell transformation of non-malignant human skin keratinocyte, and colony growth of malignant melanoma (MM) cells in soft agar. Importantly, activated and total RSK2 protein levels are highly detected in human skin cancer tissues including squamous cell carcinoma, basal-cell carcinoma, and MM. Kaempferol and eriodictyol are natural substances to inhibit kinase activity of the RSK2 N-terminal kinase domain, which is a critical kinase domain to transduce their activation signals to the substrates by phosphorylation. In this review, we discuss the role of RSK2 in skin cancer, particularly in activation of signaling pathways and potent natural substances to target RSK2 as chemopreventive and therapeutic agents.
Palm Vein Recognition and Verification System Using Local Average of Vein Direction

During the last years, hand vein patterns recognition is one of the most recent biometric technologies used for the identification/verification of individuals. The vein trace is hard to damaged, changed or falsified since veins are internal to the human body. In this paper a novel palm vein recognition and verification system is presented. The first step in the proposed system is image enhancement and localization of veins grid which is a major challenge due to poor quality of veins images. The second challenging task is the palm vein feature extraction. In this research the spatial distribution of local averages of veins direction is introduced and used as feature vector. The system was tested over a database collected from 250 volunteers, where 24 images for the 2 palms are collected for each person. In total, a database contains 6,000 images belong to 500 different palms. The attained identification result is encouraging (99.95%). The verification tests indicated the achieved minimum equal error rate (EER) is 0.24%.
Cost-Effectiveness of Treating Multidrug-Resistant Tuberculosis

Background Despite the existence of effective drug treatments, tuberculosis (TB) causes 2 million deaths annually worldwide. Effective treatment is complicated by multidrug-resistant TB (MDR TB) strains that respond only to second-line drugs. We projected the health benefits and cost-effectiveness of using drug susceptibility testing and second-line drugs in a lower-middle-income setting with high levels of MDR TB. Methods and Findings We developed a dynamic state-transition model of TB. In a base case analysis, the model was calibrated to approximate the TB epidemic in Peru, a setting with a smear-positive TB incidence of 120 per 100,000 and 4.5% MDR TB among prevalent cases. Secondary analyses considered other settings. The following strategies were evaluated: first-line drugs administered under directly observed therapy (DOTS), locally standardized second-line drugs for previously treated cases (STR1), locally standardized second-line drugs for previously treated cases with test-confirmed MDR TB (STR2), comprehensive drug susceptibility testing and individualized treatment for previously treated cases (ITR1), and comprehensive drug susceptibility testing and individualized treatment for all cases (ITR2). Outcomes were costs per TB death averted and costs per quality-adjusted life year (QALY) gained. We found that strategies incorporating the use of second-line drug regimens following first-line treatment failure were highly cost-effective compared to strategies using first-line drugs only. In our base case, standardized second-line treatment for confirmed MDR TB cases (STR2) had an incremental cost-effectiveness ratio of $720 per QALY ($8,700 per averted death) compared to DOTS. Individualized second-line drug treatment for MDR TB following first-line failure (ITR1) provided more benefit at an incremental cost of $990 per QALY ($12,000 per averted death) compared to STR2. A more aggressive version of the individualized treatment strategy (ITR2), in which both new and previously treated cases are tested for MDR TB, had an incremental cost-effectiveness ratio of $11,000 per QALY ($160,000 per averted death) compared to ITR1. The STR2 and ITR1 strategies remained cost-effective under a wide range of alternative assumptions about treatment costs, effectiveness, MDR TB prevalence, and transmission. Conclusions Treatment of MDR TB using second-line drugs is highly cost-effective in Peru. In other settings, the attractiveness of strategies using second-line drugs will depend on TB incidence, MDR burden, and the available budget, but simulation results suggest that individualized regimens would be cost-effective in a wide range of situations.
Candidate Luminal B Breast Cancer Genes Identified by Genome, Gene Expression and DNA Methylation Profiling

Breast cancers (BCs) of the luminal B subtype are estrogen receptor-positive (ER+), highly proliferative, resistant to standard therapies and have a poor prognosis. To better understand this subtype we compared DNA copy number aberrations (CNAs), DNA promoter methylation, gene expression profiles, and somatic mutations in nine selected genes, in 32 luminal B tumors with those observed in 156 BCs of the other molecular subtypes. Frequent CNAs included 8p11-p12 and 11q13.1-q13.2 amplifications, 7q11.22-q34, 8q21.12-q24.23, 12p12.3-p13.1, 12q13.11-q24.11, 14q21.1-q23.1, 17q11.1-q25.1, 20q11.23-q13.33 gains and 6q14.1-q24.2, 9p21.3-p24,3, 9q21.2, 18p11.31-p11.32 losses. A total of 237 and 101 luminal B-specific candidate oncogenes and tumor suppressor genes (TSGs) presented a deregulated expression in relation with their CNAs, including 11 genes previously reported associated with endocrine resistance. Interestingly, 88% of the potential TSGs are located within chromosome arm 6q, and seven candidate oncogenes are potential therapeutic targets. A total of 100 candidate oncogenes were validated in a public series of 5,765 BCs and the overexpression of 67 of these was associated with poor survival in luminal tumors. Twenty-four genes presented a deregulated expression in relation with a high DNA methylation level. FOXO3, PIK3CA and TP53 were the most frequent mutated genes among the nine tested. In a meta-analysis of next-generation sequencing data in 875 BCs, KCNB2 mutations were associated with luminal B cases while candidate TSGs MDN1 (6q15) and UTRN (6q24), were mutated in this subtype. In conclusion, we have reported luminal B candidate genes that may play a role in the development and/or hormone resistance of this aggressive subtype.
The relative effectiveness of concept-based versus content-based video retrieval

Three video search systems were compared in the interactive search task at the TRECVID 2003 workshop: a <i>text-only</i> system, which searched video shots through transcripts; a <i>features-only</i> system, which searched video shots through 16 video content features (e.g., airplanes and people); and a <i>combined</i> system, which searched through both transcripts and content features. 36 participants each completed 12 video search tasks. The hypothesis that the combined system would perform better than both the text-only and the features-only systems was not supported, and large topic effects were found. Further analysis showed that concept-based video retrieval worked best for <i>specific</i> topics, whereas the hybrid retrieval techniques which combine both concept- and content-based video retrieval showed some advantage when searching for <i>generic</i> topics. The results have implications for topic/task analysis for video retrieval research, and also for the implementation of hybrid video retrieval systems.
A randomized controlled trial of hysterectomy or levonorgestrel-releasing intrauterine system in the treatment of menorrhagia-effect on FSH levels and menopausal symptoms.

BACKGROUND The purpose of this study was to compare the effects of hysterectomy and a levonorgestrel-releasing intrauterine system (LNG-IUS) on serum FSH levels and menopausal symptoms. METHODS A total of 236 women referred for menorrhagia to five university hospitals were randomly assigned to treatment with hysterectomy (n = 117) or LNG-IUS (n = 119). Menopausal symptoms were characterized by the Kupperman menopausal distress test. Serum FSH and estradiol levels were measured at baseline and 6 and 12 months after hysterectomy or application of LNG-IUS. Analyses were by intention to treat. RESULTS After 6 months, there was no difference between the groups, but 12 months after follow-up hysterectomized women had higher FSH levels than women with LNG-IUS (P = 0.005). There was a significant association between FSH levels and treatment modality (P = 0.020). Hot flushes increased significantly in the hysterectomy group (P = 0.02). There was a significant association between hot flushes and both treatment modality and age (P = 0.02 and P = 0.01, respectively). CONCLUSION Hysterectomy may impair ovarian function shown by rising serum FSH levels and hot flushes. However, these results should be interpreted with caution, and longer follow-up is needed.
Relationship between stomatal density, size and speed of opening in Sumatran rainforest species

Recent studies have suggested that an association between size and speed of stomatal opening of stomata within taxa is likely to play a role in photosynthesis and transpiration. In this study we investigate whether this correlation applies for seedlings of 11 rainforest species from different taxa, and whether differences in stomatal and gas exchange parameters were related to initial growth under field and controlled conditions. The experiment was conducted on seedlings of nine late successional species and two early successional species, placed in full sunlight or 70% shade. We assessed density, size, length and width of guard cells, coupled with gas exchange parameters in the transition from darkness to light, recording minimum stomatal conductance during daytime darkness (gs-dark), operating maximum stomatal conductance (gs-op), speed of stomatal opening and the time to reach 50% conductance (T-50%). All stomata and gas exchange parameters were different between species. Shade significantly affected size and density, and all gas exchange parameters except gs-op were different between light situations. Stomatal size correlated negatively with speed of opening and positively with T-50%, confirming that smaller stomata open faster than large stomata. The two early successional species were very different in stomatal size and density, and in response to light. Anatomic parameters and physiological traits were not related to height growth, but gs-dark, gs-op and speed of stomatal opening were associated with biomass growth in a subselection of six late successional species.
Effect of digital marketing mix and brand to buying intention on the motorcycle market

The Change of marketing mix to Digital has caused the complexity of marketing. This research shows the influence of the Digital marketing mix which has transformed into 4Es and Brand as Experience, Exchange, Everywhere, Evangelism, Brand Distinctiveness, and Brand Attractiveness via Brand Attitude to Buying Intention on motorcycle market case study Honda Thailand.
Comparison of sevoflurane with propofol for laryngeal mask airway insertion in adults.

UNLABELLED We performed a prospective, randomized, controlled trial to compare the quality and ease of laryngeal mask airway (LMA) insertion after either rapid inhaled sevoflurane or i.v. propofol induction of anesthesia. Seventy-six unpremedicated ASA physical status I or II patients were anesthetized with either a single vital capacity breath of sevoflurane 8% or i.v. propofol 3 mg/kg, which produced equally rapid loss of consciousness (40.5 +/- 13.9 vs 37.7 +/- 9.9 s; P > 0.05). The LMA was inserted more rapidly in patients in the propofol group (74 +/- 29 vs 127 +/- 35 s; P < 0.01) and required fewer attempts (1.2 vs 1.6; P < 0.05) than the sevoflurane group. There was a greater incidence of initially impossible mouth opening in the sevoflurane group (45% vs 21%; P < 0.05). Once mouth opening was possible, the degree of attenuation of laryngeal reflexes was similar. The overall incidence of complications related to LMA insertion, especially apnea (32% vs 0%; P < 0.01), was more frequent in the propofol group (82% vs 26%; P < 0.01). There were four failures of LMA insertion in the propofol group and none in the sevoflurane group. Both groups had stable hemodynamic profiles and good patient satisfaction. We conclude that sevoflurane vital capacity breath induction compares favorably with i.v. propofol induction for LMA insertion in adults. However, prolonged jaw tightness after the sevoflurane induction of anesthesia may delay LMA insertion. IMPLICATIONS In this randomized, controlled trial, we compared the ease of insertion of the laryngeal mask airway in adults after induction of anesthesia with either a sevoflurane vital capacity breath technique or propofol i.v. We conclude that sevoflurane compares favorably with propofol, although prolonged jaw tightness may delay laryngeal mask airway insertion.