Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular mechanisms of hyperglycemia and cardiovascular-related events in critically ill patients: rationale for the clinical benefits of insulin therapy

Newly recognized hyperglycemia frequently occurs with acute medical illness, especially among patients with cardiovascular disease (CVD). Hyperglycemia has been linked to increased morbidity and mortality in critically ill patients, especially when it is newly recognized. Increased rates of reinfarction, rehospitalization, major cardiovascular events, and death in CVD patients have also been found. An expanding body of literature describes the benefits of normalizing hyperglycemia with insulin therapy in hospitalized patients. This article reviews several underlying mechanisms thought to be responsible for the association between hyperglycemia and poor outcomes in critically ill patients and those with cardiovascular events, as well as the biologic rationale for the benefits of insulin therapy in these patients.
Targeting the CaMKII/ERK Interaction in the Heart Prevents Cardiac Hypertrophy

Aims Activation of Ca2+/Calmodulin protein kinase II (CaMKII) is an important step in signaling of cardiac hypertrophy. The molecular mechanisms by which CaMKII integrates with other pathways in the heart are incompletely understood. We hypothesize that CaMKII association with extracellular regulated kinase (ERK), promotes cardiac hypertrophy through ERK nuclear localization. Methods and Results In H9C2 cardiomyoblasts, the selective CaMKII peptide inhibitor AntCaNtide, its penetratin conjugated minimal inhibitory sequence analog tat-CN17β, and the MEK/ERK inhibitor UO126 all reduce phenylephrine (PE)-mediated ERK and CaMKII activation and their interaction. Moreover, AntCaNtide or tat-CN17β pretreatment prevented PE induced CaMKII and ERK nuclear accumulation in H9C2s and reduced the hypertrophy responses. To determine the role of CaMKII in cardiac hypertrophy in vivo, spontaneously hypertensive rats were subjected to intramyocardial injections of AntCaNtide or tat-CN17β. Left ventricular hypertrophy was evaluated weekly for 3 weeks by cardiac ultrasounds. We observed that the treatment with CaMKII inhibitors induced similar but significant reduction of cardiac size, left ventricular mass, and thickness of cardiac wall. The treatment with CaMKII inhibitors caused a significant reduction of CaMKII and ERK phosphorylation levels and their nuclear localization in the heart. Conclusion These results indicate that CaMKII and ERK interact to promote activation in hypertrophy; the inhibition of CaMKII-ERK interaction offers a novel therapeutic approach to limit cardiac hypertrophy.
Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits

Vertebrate organisms adapt to a continuously changing environment by regulating the strength of synaptic connections between brain cells. Excitatory synapses are believed to increase their strength by vesicular insertion of transmitter glutamate receptors into the postsynaptic plasma membrane. These vesicles, however, have never been demonstrated or characterized. For the first time, we show the presence of small vesicles in postsynaptic spines, often closely adjacent to the plasma membrane and PSD (postsynaptic density). We demonstrate that they harbor vesicle-associated membrane protein 2 (VAMP2/synaptobrevin-2) and glutamate receptor subunit 1 (GluA1). Disrupting VAMP2 by tetanus toxin treatment reduces the concentration of GluA1 in the postsynaptic plasma membrane. GluA1/VAMP2-containing vesicles, but not GluA2/VAMP2-vesicles, are concentrated in postsynaptic spines relative to dendrites. Our results indicate that small postsynaptic vesicles containing GluA1 are inserted directly into the spine plasma membrane through a VAMP2-dependent mechanism.
An adaptive MAC protocol with enhanced QoS-guarantee capability for 3.5G TD-CDMA-based wireless communication systems

We propose a differentiated CDMA (D-CDMA) protocol for 3.5/sup th/-generation (3.5G) wireless cellular networks. D-CDMA employs an adaptive channel access scheme and a differentiated frame structure to support QoS differentiation and guarantee. From our simulation and analytical results, we show that D-CDMA with penalty-based adaptable reservation and admission (PARA) can achieve smaller blocking rate, given an arrival rate, and higher throughput, given a blocking rate objective, as compared to previous protocols without PARA.
Reconstruction-Based Recognition of Scenes with Translationally Repeated Quadrics

This paper addresses the problem of invariant-based recognition of quadric configurations from a single image. These configurations consist of a pair of rigidly connected translationally repeated quadric surfaces. This problem is approached via a reconstruction framework. A new mathematical framework, using relative affine structure, on the lines of Luong and Vieville (1996), has been proposed. Using this mathematical framework, translationally repeated objects have been projectively reconstructed, from a single image, with four image point correspondences of the distinguished points on the object and its translate. This has been used to obtain a reconstruction of a pair of translationally repeated quadrics. We have proposed joint projective invariants of a pair of proper quadrics. For the purpose of recognition of quadric configurations, we compute these invariants for the pair of reconstructed quadrics. Experimental results on synthetic and real images, establish the discriminatory power and stability of the proposed invariant-based recognition strategy. As a specific example, we have applied this technique for discriminating images of monuments which are characterized by translationally repeated domes modeled as quadrics.
Vocal and visual stimulation, congruence and lateralization affect brain oscillations in interspecies emotional positive and negative interactions

The present research explored the effect of cross-modal integration of emotional cues (auditory and visual (AV)) compared with only visual (V) emotional cues in observing interspecies interactions. The brain activity was monitored when subjects processed AV and V situations, which represented an emotional (positive or negative), interspecies (human–animal) interaction. Congruence (emotionally congruous or incongruous visual and auditory patterns) was also modulated. electroencephalography brain oscillations (from delta to beta) were analyzed and the cortical source localization (by standardized Low Resolution Brain Electromagnetic Tomography) was applied to the data. Frequency band (mainly low-frequency delta and theta) showed a significant brain activity increasing in response to negative compared to positive interactions within the right hemisphere. Moreover, differences were found based on stimulation type, with an increased effect for AV compared with V. Finally, delta band supported a lateralized right dorsolateral prefrontal cortex (DLPFC) activity in response to negative and incongruous interspecies interactions, mainly for AV. The contribution of cross-modality, congruence (incongruous patterns), and lateralization (right DLPFC) in response to interspecies emotional interactions was discussed at light of a “negative lateralized effect. ”
Fathering and Child Outcomes

ABOUT THE AUTHOR. FOREWORD BY ANN BUCHANAN. Chapter 1 Fathering: A Changing Perspective. Chapter 2 Factors Associated with Fathers' Involvement in Two-Parent Families. Chapter 3 Fathers' Involvement and Children's Mental Health Outcomes. Chapter 4 Fathers' Involvement and Children's Educational Outcomes. Chapter 5 Fathers' Involvement and Adolescent Aggressive Behaviour. Chapter 6 Fathers' Involvement and Children's Quality of Relationships. Chapter 7 Fathers' Involvement and Children's Socio-Economic Outcomes. Chapter 8 Non-Resident Fathers' Parenting: Determinants and Children's Psychological Outcomes. Chapter 9 Conclusions. REFERENCES. INDEX.
Gene Expression during the Generation and Activation of Mouse Neutrophils: Implication of Novel Functional and Regulatory Pathways

As part of the Immunological Genome Project (ImmGen), gene expression was determined in unstimulated (circulating) mouse neutrophils and three populations of neutrophils activated in vivo, with comparison among these populations and to other leukocytes. Activation conditions included serum-transfer arthritis (mediated by immune complexes), thioglycollate-induced peritonitis, and uric acid-induced peritonitis. Neutrophils expressed fewer genes than any other leukocyte population studied in ImmGen, and down-regulation of genes related to translation was particularly striking. However, genes with expression relatively specific to neutrophils were also identified, particularly three genes of unknown function: Stfa2l1, Mrgpr2a and Mrgpr2b. Comparison of genes up-regulated in activated neutrophils led to several novel findings: increased expression of genes related to synthesis and use of glutathione and of genes related to uptake and metabolism of modified lipoproteins, particularly in neutrophils elicited by thioglycollate; increased expression of genes for transcription factors in the Nr4a family, only in neutrophils elicited by serum-transfer arthritis; and increased expression of genes important in synthesis of prostaglandins and response to leukotrienes, particularly in neutrophils elicited by uric acid. Up-regulation of genes related to apoptosis, response to microbial products, NFkB family members and their regulators, and MHC class II expression was also seen, in agreement with previous studies. A regulatory model developed from the ImmGen data was used to infer regulatory genes involved in the changes in gene expression during neutrophil activation. Among 64, mostly novel, regulatory genes predicted to influence these changes in gene expression, Irf5 was shown to be important for optimal secretion of IL-10, IP-10, MIP-1α, MIP-1β, and TNF-α by mouse neutrophils in vitro after stimulation through TLR9. This data-set and its analysis using the ImmGen regulatory model provide a basis for additional hypothesis-based research on the importance of changes in gene expression in neutrophils in different conditions.
Cyto protective effect of bis[benzyl N′-(indol-3-ylmethylene)-hydrazinecarbodithioato]-nickel(II) against ethanol-induced gastric mucosal injury in rats

Bis[benzyl N′-(indol-3-ylmethylene)-hydrazinecarbodithioato]-nickel(II) (BIHCN) has been used in traditional medicine for their biological activities. The present study was performed to evaluate the anti-ulcerogenic activity of BIHCN against ethanol induced gastric mucosal injury in rats. Six groups of adult Sprague Dawley rats were orally pre-treated respectively with 10% Tween 20 solution (ulcer control group), omeprazole 20 mg/kg (reference group), and 50, 100, 200 and 400 mg/kg of BIHCN in 10% Tween 20 solution (experimental groups) one hour before oral administration of absolute ethanol to generate gastric mucosal injury. After an additional hour, the rats were sacrificed and the ulcer areas of the gastric walls were determined. Grossly, the ulcer control group exhibited severe mucosal injury, whereas pre-treatment with compound exhibited significant protection of gastric mucosal injury. Histological studies of the gastric wall revealed that ulcer control group exhibited severe damage of gastric mucosa; along with edema and leucocytes infiltration of submucosal layer compared to rats pre-treated with BIHCN which showed comparatively gastric mucosal protection, reduction or absence of edema and leucocytes infiltration of submucosal layer. Acute toxicity study of BIHCN with a higher dose of 5 g/kg did not manifest any toxicological signs in rats. In conclusions, the present finding suggests that BIHCN promotes ulcer protection as ascertained grossly by significant reduction of ulcer area, and histologically by comparatively decreases in ulcer areas, reduction or absence of edema and leucocytes infiltration of submucosal layer compared to ulcer control group.
Major Surface Glycoproteins of Insect Forms of Trypanosoma brucei Are Not Essential for Cyclical Transmission by Tsetse

Procyclic forms of Trypanosoma brucei reside in the midgut of tsetse flies where they are covered by several million copies of glycosylphosphatidylinositol-anchored proteins known as procyclins. It has been proposed that procyclins protect parasites against proteases and/or participate in tropism, directing them from the midgut to the salivary glands. There are four different procyclin genes, each subject to elaborate levels of regulation. To determine if procyclins are essential for survival and transmission of T. brucei, all four genes were deleted and parasite fitness was compared in vitro and in vivo. When co-cultured in vitro, the null mutant and wild type trypanosomes (tagged with cyan fluorescent protein) maintained a near-constant equilibrium. In contrast, when flies were infected with the same mixture, the null mutant was rapidly overgrown in the midgut, reflecting a reduction in fitness in vivo. Although the null mutant is patently defective in competition with procyclin-positive parasites, on its own it can complete the life cycle and generate infectious metacyclic forms. The procyclic form of T. brucei thus differs strikingly from the bloodstream form, which does not tolerate any perturbation of its variant surface glycoprotein coat, and from other parasites such as Plasmodium berghei, which requires the circumsporozoite protein for successful transmission to a new host.
The Role of Cognitive Fit in the Relationship Between Software Comprehension and Modification

Although there is a long tradition of empirical studies of software developers, few studies have focused on software maintenance. Prior work is predicated on the belief that higher levels of software comprehension are associated with higher levels of performance on modification tasks. This study provides a more complete understanding of the relationship between software comprehension and modification. We conceptualize software maintenance as interlinking comprehension and modification, and argue that the relationship between the two is moderated by cognitive fit. Specifically, cognitive fit exists when the software maintainer's dominant mental representation of the software and their mental representation of the modification task emphasize the same type of knowledge. We hypothesize that when cognitive fit exists, greater improvements in comprehension are associated with higher levels of performance on a modification task. When cognitive fit does not exist, however, the software maintainer's mental representations of the software and of the modification task do not emphasize the same type of knowledge, which may mean that attention is devoted to comprehension at the expense of modification, resulting in lower performance on the modification task. In these circumstances, comprehension and modification tasks may interfere with each other, an effect known as dual-task interference. We therefore hypothesize that performance on a modification task is moderated by the fit between the mental representation of the software and that of the modification task. We tested our theory by varying cognitive fit to create matched and mismatched conditions in a single experiment that used IT professionals as subjects. Our findings support our theory: cognitive fit moderates the relationship between comprehension and modification. Specifically, changes in software comprehension and modification performance are positively related when cognitive fit exists and negatively related when cognitive fit does not exist. Our findings demonstrate the need to examine more complex relationships among the numerous types of tasks involved in software development rather than examining software comprehension alone.
Regulation of Glyoxylate Metabolism in Escherichia coli K-12

The relative contributions of the dicarboxylic acid and the tricarboxylic acid cycles to the oxidative catabolism of glyoxylate in Escherichia coli K-12 were deduced by analysis of mutant strains that were blocked in the formation of glyoxylate carboligase and of malate synthase G (the “glycolate form” of malate synthase). Mutant strains unable to form malate synthase G were unimpaired in their ability to oxidize glyoxylate. Hence, the dicarboxylic acid cycle does not appear to play an essential role in this process. Organisms blocked in the synthesis of glyoxylate carboligase did not oxidize glyoxylate at a detectable rate, indicating that wild-type organisms convert glyoxylate to acetyl-coenzyme A and oxidize it via the tricarboxylic acid cycle. The foregoing evidence indicates that malate synthase G plays an anaplerotic role during growth with glycolate or acetate as the carbon source. The in vivo activity of malate synthase G was not detectable when the intracellular concentration of acetyl-coenzyme A was low, suggesting that this substrate or a closely related metabolite exerts a sensitive positive control over the enzyme. The synthesis of malate synthase G appears to be induced directly by glycolate which may be formed by a constitutive reduced nicotinamide adenine dinucleotide phosphate-dependent glyoxylate reductase in glyoxylate- or acetate-grown cells.
Loneliness and social isolation among older Irish people

The Council is pleased that the results of this research dispel an enduring stereotype of old age by demonstrating that the majority of a representative sample of older people is not socially isolated or lonely. Furthermore, the findings demonstrate the importance of being able to adapt to transition times in older age and the necessity for economic, social and personal resources to facilitate this adaptation and to promote successful ageing. Finally, the results highlight how critical it is for older people to be able to get out of the house and the Council urges that the recommendations that it has made in this regard be acted on in the short-term in order to ensure that older people do not become socially excluded in their third and fourth ages.
Dose-Dependent AMPK-Dependent and Independent Mechanisms of Berberine and Metformin Inhibition of mTORC1, ERK, DNA Synthesis and Proliferation in Pancreatic Cancer Cells

Natural products represent a rich reservoir of potential small chemical molecules exhibiting anti-proliferative and chemopreventive properties. Here, we show that treatment of pancreatic ductal adenocarcinoma (PDAC) cells (PANC-1, MiaPaCa-2) with the isoquinoline alkaloid berberine (0.3–6 µM) inhibited DNA synthesis and proliferation of these cells and delay the progression of their cell cycle in G1. Berberine treatment also reduced (by 70%) the growth of MiaPaCa-2 cell growth when implanted into the flanks of nu/nu mice. Mechanistic studies revealed that berberine decreased mitochondrial membrane potential and intracellular ATP levels and induced potent AMPK activation, as shown by phosphorylation of AMPK α subunit at Thr-172 and acetyl-CoA carboxylase (ACC) at Ser79. Furthermore, berberine dose-dependently inhibited mTORC1 (phosphorylation of S6K at Thr389 and S6 at Ser240/244) and ERK activation in PDAC cells stimulated by insulin and neurotensin or fetal bovine serum. Knockdown of α1 and α2 catalytic subunit expression of AMPK reversed the inhibitory effect produced by treatment with low concentrations of berberine on mTORC1, ERK and DNA synthesis in PDAC cells. However, at higher concentrations, berberine inhibited mitogenic signaling (mTORC1 and ERK) and DNA synthesis through an AMPK-independent mechanism. Similar results were obtained with metformin used at doses that induced either modest or pronounced reductions in intracellular ATP levels, which were virtually identical to the decreases in ATP levels obtained in response to berberine. We propose that berberine and metformin inhibit mitogenic signaling in PDAC cells through dose-dependent AMPK-dependent and independent pathways.
Understanding Voltage Variations in Chip Multiprocessors using a Distributed Power-Delivery Network

Recent efforts to address microprocessor power dissipation through aggressive supply voltage scaling and power management require that designers be increasingly cognizant of power supply variations. These variations, primarily due to fast changes in supply current, can be attributed to architectural gating events that reduce power dissipation. In order to study this problem, the authors propose a fine-grain, parameterizable model for power-delivery networks that allows system designers to study localized, on-chip supply fluctuations in high-performance microprocessors. Using this model, the authors analyze voltage variations in the context of next-generation chip-multiprocessor (CMP) architectures using both real applications and synthetic current traces. They find that the activity of distinct cores in CMPs present several new design challenges when considering power supply noise, and they describe potentially problematic activity sequences that are unique to CMP architectures
Outcome of Vaginoplasty in Male-to-Female Transgenders: A Systematic Review of Surgical Techniques.

INTRODUCTION Gender reassignment surgery is the keystone of the treatment of transgender patients. For male-to-female transgenders, this involves the creation of a neovagina. Many surgical methods for vaginoplasty have been opted. The penile skin inversion technique is the method of choice for most gender surgeons. However, the optimal surgical technique for vaginoplasty in transgender women has not yet been identified, as outcomes of the different techniques have never been compared. AIM With this systematic review, we aim to give a detailed overview of the published outcomes of all currently available techniques for vaginoplasty in male-to-female transgenders. METHODS A PubMed and EMBASE search for relevant publications (1995-present), which provided data on the outcome of techniques for vaginoplasty in male-to-female transgender patients. MAIN OUTCOME MEASURES Main outcome measures are complications, neovaginal depth and width, sexual function, patient satisfaction, and improvement in quality of life (QoL). RESULTS Twenty-six studies satisfied the inclusion criteria. The majority of these studies were retrospective case series of low to intermediate quality. Outcome of the penile skin inversion technique was reported in 1,461 patients, bowel vaginoplasty in 102 patients. Neovaginal stenosis was the most frequent complication in both techniques. Sexual function and patient satisfaction were overall acceptable, but many different outcome measures were used. QoL was only reported in one study. Comparison between techniques was difficult due to the lack of standardization. CONCLUSIONS The penile skin inversion technique is the most researched surgical procedure. Outcome of bowel vaginoplasty has been reported less frequently but does not seem to be inferior. The available literature is heterogeneous in patient groups, surgical procedure, outcome measurement tools, and follow-up. Standardized protocols and prospective study designs are mandatory for correct interpretation and comparability of data.