Miracle-dz/UniFAR-FTUs huggingface dataset:
The mediating role of anxiety to perform social skills between parental attachment and adolescents' self-efficacy

The present study examined the mediating role of adolescent’s anxiety to perform social skills on the relationships between perceived parental attachment and self-efficacy among 374 Iranian adolescents aged 17 to 19 years old. Survey results showed that parental attachment was significantly related to an adolescent’s self-efficacy (p<0.05). Moreover, the results revealed that adolescents with low self-efficacy experienced higher anxiety to perform social skills (p<0.05). Anxiety to perform social skills mediated the relationship between parental attachment and adolescents’ self-efficacy (p<0.05). The findings highlighted the mediating role of anxiety to perform social skills on the relationship between parental attachment and adolescent’s self-efficacy.
Leishmania-Derived Murine Monocyte Chemoattractant Protein 1 Enhances the Recruitment of a Restrictive Population of CC Chemokine Receptor 2-Positive Macrophages

ABSTRACT Transgenic Leishmania parasites that encode the murine chemokine monocyte chemoattractant protein 1 (MCP-1) were generated. These parasites transcribed MCP-1 mRNA and secreted MCP-1 protein. Infection of BALB/c, C57BL/6, or MCP-1 knockout (KO) mice with these parasites resulted in minimal lesion development with fewer parasites in the infected foot, lymph node, and spleen compared to wild-type-infected mice. In contrast, transgenic parasites caused substantial lesions with relatively high numbers of parasites in CC chemokine receptor 2 (CCR2) KO mice, indicating that the parasites are viable and healthy and that the lack of lesion development is CCR2 dependent. Prior infection of mice with transgenic parasites offered no protection to subsequent wild-type L. major challenge, suggesting that the transgenic parasites are controlled by an early innate immune response. Consistent with innate immunity, flow cytometry of cells from the ears of mice infected with transgenic parasites revealed an increase in the number of CCR2-positive macrophages by day 7 postinfection. The enumeration of transgenic parasites in ear lesions demonstrated a significant reduction in parasite numbers, which coincided with the increased CCR2-positive macrophage migration. CCR2-positive macrophages isolated from ears of mice infected with transgenic parasites contained virtually no parasites. In vitro studies revealed that optimal parasite killing required the recruitment of CCR2-positive macrophages, followed by stimulation with a combination of both MCP-1 and gamma interferon (IFN-γ). This work suggests that the parasite-derived MCP-1 can recruit a restrictive population of CCR2-positive macrophages into lesions that can be optimally stimulated by MCP-1 and IFN-γ to efficiently kill Leishmania parasites.
Abnormal mitochondrial fusion–fission balance contributes to the progression of experimental sepsis

Abstract Sepsis-associated multiple organ failure is a major cause of mortality characterized by a massive increase of reactive oxygen and nitrogen species (ROS/RNS) and mitochondrial dysfunction. Despite intensive research, determining events in the progression or reversal of the disease are incompletely understood. Herein, we studied two prototype sepsis models: endotoxemia and cecal ligation and puncture (CLP)—which showed very different lethality rates (2.5% and 67%, respectively)—, evaluated iNOS, ROS and respiratory chain activity, and investigated mitochondrial biogenesis and dynamics, as possible processes involved in sepsis outcome. Endotoxemia and CLP showed different iNOS, ROS/RNS, and complex activities time-courses. Moreover, these alterations reverted after 24-h endotoxemia but not after CLP. Mitochondrial biogenesis was not elicited during the first 24 h in either model but instead, 50% mtDNA depletion was observed. Mitochondrial fusion and fission were evaluated using real-time PCR of mitofusin-2 (Mfn2), dynamin-related protein-1 (Drp1), and using electron microscopy. During endotoxemia, we observed a decrease of Mfn2-mRNA levels at 4–6 h, and an increase of mitochondrial fragmentation at 6 h. These parameters reverted at 24 h. In contrast, CLP showed not only decreased Mfn2-mRNA levels at 12–18 h but also increased Drp1-mRNA levels at 4 h, and enhanced and sustained mitochondrial fragmentation. The in vivo pretreatment with mdivi-1 (Drp1 inhibitor) significantly attenuated mitochondrial dysfunction and apoptosis in CLP. Therefore, abnormal fusion-to-fission balance, probably evoked by ROS/RNS secondary to iNOS induction, contributes to the progression of sepsis. Pharmacological targeting of Drp1 may be a potential novel therapeutic tool for sepsis.
Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation

The exit of a stem cell out of quiescence into an activated state is characterized by major metabolic changes associated with increased biosynthesis of proteins and macromolecules. The regulation of this transition is poorly understood. Using muscle stem cells, or satellite cells (SCs), we found that autophagy, which catabolizes intracellular contents to maintain proteostasis and to produce energy during nutrient deprivation, was induced during SC activation. Inhibition of autophagy suppressed the increase in ATP levels and delayed SC activation, both of which could be partially rescued by exogenous pyruvate as an energy source, suggesting that autophagy may provide nutrients necessary to meet bioenergetic demands during this critical transition from quiescence to activation. We found that SIRT1, a known nutrient sensor, regulates autophagic flux in SC progeny. A deficiency of SIRT1 led to a delay in SC activation that could also be partially rescued by exogenous pyruvate. These studies suggest that autophagy, regulated by SIRT1, may play an important role during SC activation to meet the high bioenergetic demands of the activation process.
Mining User Intents in Twitter: A Semi-Supervised Approach to Inferring Intent Categories for Tweets

In this paper, we propose to study the problem of identifying and classifying tweets into intent categories. For example, a tweet “I wanna buy a new car” indicates the user’s intent for buying a car. Identifying such intent tweets will have great commercial value among others. In particular, it is important that we can distinguish different types of intent tweets. We propose to classify intent tweets into six categories, namely Food & Drink, Travel, Career & Education, Goods & Services, Event and Activities and Trifle. We propose a semisupervised learning approach to categorizing intent tweets into the six categories.We construct a test collection by using a bootstrap method. Our experimental results show that our approach is effective in inferring intent categories for tweets.
Seasonal and Regional Variations of Active/Passive Microwave Signatures of Sea Ice

Ku-band (13.3 GHz) scatterometer and K-band (19.4 GHz) radiometer data acquired by the CCRS CV-580 aircraft over the period from 1979 to 1982 in Canadian and Danish (Greenland) coastal waters have been analyzed to determine the seasonal and regional variations of microwave sea-ice signatures. A clustering analysis of the like and cross-polarized scattering cross sections, ¿HHo and ¿HVo, and the H polarized emissivity ¿H, has been used to identify distinct microwave sea-ice signatures for each ice type and to trace the evolution of these signatures with region and season. Ice-type signatures in the high Arctic under cold conditions are quite stable, and major ice classes are readily identified from microwave measurements. Under warmer conditions the signatures change with the structure, moisture content of the snow pack, and with the free water in the surface layers of the underlying ice. An attempt is made to create a consistent picture of the microwave signature transformation by grouping the data into " seaice seasons" (snow and ice surface transformation stages). The separation between microwave ice-class signatures reaches a minimum at the peak of the summer melt.
In vitro and In vivo Radiosensitization with AZD6244 (ARRY-142886), an Inhibitor of Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase 1/2 Kinase

Purpose: The mitogen-activated protein (MAP) kinase pathway is important for cell proliferation, survival, and differentiation, and is frequently up-regulated in cancers. The MAP kinase pathway is also activated after exposure to ionizing radiation. We investigated the effects of AZD6244 (ARRY-142886), an inhibitor of MAP kinase/extracellular signal-regulated kinase 1/2, on radiation response. Experimental Design: The effects of AZD6244 on the in vitro radiosensitivity of human cancer cell lines (A549, MiaPaCa2, and DU145) were evaluated using clonogenic assays. DNA damage repair was evaluated using γH2AX, and mitotic catastrophe was measured using nuclear fragmentation. Cell cycle effects were measured with flow cytometry. Growth delay was used to evaluate the effects of AZD6244 on in vivo tumor radiosensitivity. Results: Exposure of each cell line to AZD6244 before irradiation resulted in an increase in radiosensitivity with dose enhancement factors at a surviving fraction of 0.1, ranging from 1.16 to 2.0. No effects of AZD6244 on radiation-induced apoptosis or persistence of γH2AX foci after irradiation were detected. Cells treated with AZD6244 had an increased mitotic index and decreased Chk1 phosphorylation at 1 and 2 hours after irradiation. Mitotic catastrophe was increased in cells receiving AZD6244 and irradiation compared with the single treatments. In vivo studies revealed that AZD6244 administration to mice bearing A549 tumor xenografts resulted in a greater than additive increase in radiation-induced tumor growth delay (dose enhancement factor of 3.38). Conclusions: These results indicate that AZD6244 can enhance tumor cell radiosensitivity in vitro and in vivo and suggest that this effect involves an increase in mitotic catastrophe.
Shielding of High Magnetic Fields

This paper revisits the problem of shielding low- frequency high magnetic fields. Such fields are encountered in the domain of electromechanical energy conversion in particular during railgun operation. Using finite-element (FEM) analysis, we investigate, separately, the mechanism of redirection of the magnetic flux due to induced eddy-currents and the magnetic flux shunting due to highly permeable materials. In a first phase, various shapes of highly conductive shields are analyzed in the frequency domain. In a second phase, we study the shunt effect of different shields being made of magnetic materials. In a magnetostatic model afterwards, for a shield that combines both highly conductive and magnetically highly permeable materials, the case of a double-exponential shaped magnetic field is treated in the time domain. Finally, we make some remarks on the concept of shielding effectiveness.
Experimental Study of Normal- and High-Strength Concrete Confined with Fiber-Reinforced Polymers

The experimental program reported here was conducted to gain insight into the behavior of concrete confined with fiber-reinforced polymers (FRPs). A total of 112 cylindrical concrete specimens, each 150 mm in diameter, 300 mm in height, and concrete strength up to 112 MPa, were tested under monotonic uniaxial compression. Test variables included amount of FRP, strength and stiffness of FRP, concrete strength, and the health of concrete at the time of strengthening. Results showed that, with an increase of the unconfined concrete strength, the strength enhancement, energy absorption capacity, ductility factor, and work (energy) index at rupture of FRP jackets all decreased remarkably. A positive correlation was found between concrete ductility and FRP rupture strain. A gradual post-peak failure of the specimens, observed previously from FRP-confined concrete columns tested at the University of Toronto, was also observed in some of the current tests. This ductile failure, attributed to the gradual unzipping failure of FRP jacket, is related to specimen size and is explained in terms of various confinement parameters.
Improving Spatial Reuse in Multihop Wireless Networks - A Survey

In multihop wireless ad-hoc networks, the medium access control (MAC) protocol plays a key role in coordinating the access to the shared medium among wireless nodes. Currently, the distributed coordination function (DCF) of the IEEE 802.11 is the dominant MAC protocol for both wireless LANs and wireless multihop ad hoc environment due to its simple implementation and distributed nature. The current access method of the IEEE 802.11 does not make efficient use of the shared channel due to its conservative approach in assessing the level of interference; this in turn affects the spatial reuse of the limited radio resources and highly affect the achieved throughput of a multihop wireless network. This paper surveys various methods that have been proposed in order to enhance the channel utilization by improving the spatial reuse.
Structural Integrity Assessment of Precast Concrete Slabs Employing Conventional and Recycled Coarse Aggregates via Vibration Tests

Precast slabs were tested, consisting of ceramic blocks supported by concrete ribs and with a concrete topping a few centimeters thick. These structures are very common in Brazil, being employed for the construction of houses and small buildings. In one of the tested slabs, recycled coarse aggregate was employed while conventional aggregate (granite gravels and quartz sand) was employed in the other tested slab. This study is part of a broader experimental programme which was designed to assess the applicability of concrete containing recycled aggregates as conventional aggregate replacement. In order to get insight into the structural behavior of slabs with recycled aggregates, the main focus of this paper is to assess the effect of structural load level on vibration parameters (Resonance Frequency, Damping Ratio and Transit Time). Full scale slab structural integrity was assessed by following changes in these parameters via a vibration (modal) test employing impact excitation. The structures were tested for several static load ratios (load to ultimate loading ratios (55%, 82% and 98%)). Visual inspection of induced cracking was also performed. The results obtained indicate a reduction of stiffness in both slabs up to 50% of the stiffness of the respective uncracked structure, which followed a similar trend despite the aggregate types. As for the damping ratios, the recycled aggregate containing slab showed lower values in comparison to the control slab with conventional aggregate. Whereas the former results might indicate similar load capacity, the latter, in principle, may have implications for the vibration serviceability limit state of structures. However, although structures with lower damping ratio can potentially present vibration problems, these studied elements are not often subjected to excessive vibration in its service life.
Proangiogenic Properties of Thrombospondin-4

Objective—Thrombospondin-4 (TSP-4) is 1 of the 5 members of the thrombospondin protein family. TSP-1 and TSP-2 are potent antiangiogenic proteins. However, angiogenic properties of the 3 other TSPs, which do not contain the domains associated with the antiangiogeneic activity of TSP-1 and TSP-2, have not been explored. In our previous studies, we found that TSP-4 is expressed in the vascular matrix of blood vessels of various sizes and is especially abundant in capillaries. We sought to identify the function of TSP-4 in the regulation of angiogenesis. Approach and Results—The effect of TSP-4 in in vivo angiogenesis models and its effect on angiogenesis-related properties in cultured cells were assessed using Thbs4−/− mice, endothelial cells (EC) derived from these mice, and recombinant TSP-4. Angiogenesis was decreased in Thbs4−/− mice compared with wild-type mice. TSP-4 was detected in the lumen of the growing blood vessels. Mice expressing the P387 TSP-4 variant, which was previously associated with coronary artery disease and found to be more active in its cellular interactions, displayed greater angiogenesis compared with A387 form. Lung EC from Thbs4−/− mice exhibited decreased adhesion, migration, and proliferation capacities compared with EC from wild-type mice. Recombinant TSP-4 promoted proliferation and the migration of EC. Integrin &agr;2 and gabapentin receptor &agr;2&dgr;-1 were identified as receptors involved in regulation of EC adhesion, migration, and proliferation by TSP-4. Conclusion—TSP-4, an extracellular matrix protein previously associated with tissue remodeling, is now demonstrated to possess proangiogenic activity.
Origins of Myc Proteins – Using Intrinsic Protein Disorder to Trace Distant Relatives

Mammalian Myc proteins are important determinants of cell proliferation as well as the undifferentiated state of stem cells and their activity is frequently deregulated in cancer. Based mainly on conservation in the C-terminal DNA-binding and dimerization domain, Myc-like proteins have been reported in many simpler organisms within and outside the Metazoa but they have not been found in fungi or plants. Several important signature motifs defining mammalian Myc proteins are found in the N-terminal domain but the extent to which these are found in the Myc-like proteins from simpler organisms is not well established. The extent of N-terminal signature sequence conservation would give important insights about the evolution of Myc proteins and their current function in mammalian physiology and disease. In a systematic study of Myc-like proteins we show that N-terminal signature motifs are not readily detectable in individual Myc-like proteins from invertebrates but that weak similarities to Myc boxes 1 and 2 can be found in the N-termini of the simplest Metazoa as well as the unicellular choanoflagellate, Monosiga brevicollis, using multiple protein alignments. Phylogenetic support for the connections of these proteins to established Myc proteins is however poor. We show that the pattern of predicted protein disorder along the length of Myc proteins can be used as a complementary approach to making dendrograms of Myc proteins that aids the classification of Myc proteins. This suggests that the pattern of disorder within Myc proteins is more conserved through evolution than their amino acid sequence. In the disorder-based dendrograms the Myc-like proteins from simpler organisms, including M. brevicollis, are connected to established Myc proteins with a higher degree of certainty. Our results suggest that protein disorder based dendrograms may be of general significance for studying distant relationships between proteins, such as transcription factors, that have high levels of intrinsic disorder.
Challenges in HIV Vaccine Research for Treatment and Prevention

Many attempts have been made or are ongoing for HIV prevention and HIV cure. Many successes are in the list, particularly for HIV drugs, recently proposed also for prevention. However, no eradication of infection has been achieved so far with any drug. Further, a residual immune dysregulation associated to chronic immune activation and incomplete restoration of B and T cell subsets, together with HIV DNA persistence in reservoirs, are still unmet needs of the highly active antiretroviral therapy, causing novel “non-AIDS related” diseases that account for a higher risk of death even in virologically suppressed patients. These “ART unmet needs” represent a problem, which is expected to increase by ART roll out. Further, in countries such as South Africa, where six millions of individuals are infected, ART appears unable to contain the epidemics. Regretfully, all the attempts at developing a preventative vaccine have been largely disappointing. However, recent therapeutic immunization strategies have opened new avenues for HIV treatment, which might be exploitable also for preventative vaccine approaches. For example, immunization strategies aimed at targeting key viral products responsible of virus transmission, activation, and maintenance of virus reservoirs may intensify drug efficacy and lead to a functional cure providing new perspectives also for prevention and future virus eradication strategies. However, this approach imposes new challenges to the scientific community, vaccine developers, and regulatory bodies, such as the identification of novel immunological and virological biomarkers to assess efficacy end-points, taking advantage from the natural history of infection and exploiting lessons from former trials. This review will focus first on recent advancement of therapeutic strategies, then on the progresses made in preventative approaches, discussing concepts, and problems for the way ahead for the development of vaccines for HIV treatment and prevention.
Disordered Eating Behaviours in an Undergraduate Sample: Associations Among Gender, Body Mass Index, and Difficulties in Emotion Regulation

The ability of individuals to recognise and regulate their emotions is known as emotion regulation. It is well established that difficulties in emotion regulation are associated with disordered eating. The present study explored the relationships among gender, body mass index, difficulties in emotion regulation, and disordered eating behaviours in a nonclinical sample of men and women. As part of a larger study, men (n 85) and women (n 165) from undergraduate university programs provided weight and height information and completed the Difficulties in Emotion Regulation Scale and the Eating Attitudes Test. Results from regression analyses suggest that, in a nonclinical sample, gender, body mass index, and specific difficulties in emotion regulation are related to disordered eating attitudes and behaviours. With respect to dieting, some of these relationships are more complex and involve interactions between BMI and both gender and impulse control.
Protein Phosphatase 1 Regulates the Histone Code for Long-Term Memory

Chromatin remodeling through histone posttranslational modifications (PTMs) and DNA methylation has recently been implicated in cognitive functions, but the mechanisms involved in such epigenetic regulation remain poorly understood. Here, we show that protein phosphatase 1 (PP1) is a critical regulator of chromatin remodeling in the mammalian brain that controls histone PTMs and gene transcription associated with long-term memory. Our data show that PP1 is present at the chromatin in brain cells and interacts with enzymes of the epigenetic machinery including HDAC1 (histone deacetylase 1) and histone demethylase JMJD2A (jumonji domain-containing protein 2A). The selective inhibition of the nuclear pool of PP1 in forebrain neurons in transgenic mice is shown to induce several histone PTMs that include not only phosphorylation but also acetylation and methylation. These PTMs are residue-specific and occur at the promoter of genes important for memory formation like CREB (cAMP response element-binding protein) and NF-κB (nuclear factor-κB). These histone PTMs further co-occur with selective binding of RNA polymerase II and altered gene transcription, and are associated with improved long-term memory for objects and space. Together, these findings reveal a novel mechanism for the epigenetic control of gene transcription and long-term memory in the adult brain that depends on PP1.