Miracle-dz/UniFAR-FTUs huggingface dataset:
Proteomic analysis revealed association of aberrant ROS signaling with suberoylanilide hydroxamic acid-induced autophagy in Jurkat T-leukemia cells

Suberoylanilide hydroxamic acid (SAHA) is a newly emerging histone deacetylase inhibitor (HDACi) and has been approved in phase II clinical trials for treating patients with cutaneous T-cell lymphoma. Autophagy is a conserved self-digestion process that degrades cytoplasmic materials and recycles long-lived proteins and organelles within cells. In this study, we demonstrate that SAHA stimulates autophagy in Jurkat T-leukemia cells, which was evidenced by the appearance of autophagic vacuoles, formation of acidic vesicular organelles, recruitment of LC3-II to the autophagosomes and conversion of LC3-I to LC3-II. Moreover, SAHA treatment upregulated expression of Beclin 1 and Atg7 and promoted formation of the Atg12-Atg5 conjugate. Furthermore, inhibition of autophagy by chloroquine (CQ) enhanced SAHA-induced apoptosis. To determine the underlying mechanism of SAHA-induced autophagy, two complementary proteomic approaches (2-DE and SILAC), coupled with ESI-Q-TOF MS/MS analysis are utilized to profile differentially expressed proteins between control and SAHA-treated Jurkat T-leukemia cells. In total, 72 proteins were identified with significant alterations. Cluster analysis of the changed proteins reveal several groups of enzymes associated with energy metabolism, anti-oxidative stress and cellular redox control, which suggested an abnormal reactive oxygen species (ROS) production in SAHA-treated Jurkat T-leukemia cells. These observations were further confirmed by ROS chemiluminescence assay. Mechanistic studies revealed that SAHA-triggered autophagy was mediated by ROS production, which could be attenuated by N-acetyl cysteine (NAC), a ROS inhibitor. Finally, we illustrated that Akt-mTOR signaling, a major suppressive cascade of autophagy, was inactivated by SAHA treatment. Taken together, our study identifies autophagy as a reaction to counter increased ROS and is thus involved as a cellular prosurvival mechanism in response to SAHA treatment.
Is There a Geography of Thought for East‐West Differences? Why or why not?

Richard Nisbett's The Geography of Thought is one of several recent works that have highlighted purported differences in thinking patterns between East Asians and Westerners on the basis of empirical research. This has implications for teaching and for other issues such as cultural integration. Based on a framework consisting of three distinct notions of rationality, this paper argues that some of the differences alleged by Nisbett are either not real or exaggerated, and that his geography of thought fails to provide an adequate account of thinking styles across cultures. The paper concludes with a discussion of the implications for teaching and learning critical thinking that can be drawn from the framework developed.
Thyroid Function in Hospitalized Patients : Effect of Illness and Serum Albumin Concentrations

10. Rodriguez-EspinosaJ, Mora-BruguesJ, Lopez-Calull C, CortesRius M. La determinacion basal deTSH per analisis inniunorradiometrico (mMA) puede hacer innecesario eluse de Ia prueba de estimulacion conTRH en el diagnostico de los estadoshipertiroideos [Letter]. Med Clin (Barcelona) 1985;85:255-6. 11. WoodBG, Waller D, Hantke U. An evaluation ofsix solid-phase thyrotropin (TSH) kits. J Clin ChemClin Biochem 1985;23:461-71. 12. Clark PMS, Price CP. Enzyme-amplified immunoassays: a new ultrasensitive assay of thyrotropin evaluated. Clin Chem 1986;32:88-92. 13. Martino B, Bambini G, Bartelena L, et al. Human serum thyrotropin measurement by ultrasensitive immunoradiometric assay as a first-line test in the evaluationof thyroid function.Clin Endocrinol (Oxford) 1986;24:141-8. 14. Kubasik NP, Same DO, Brodows RG, Sine HE. Assay of thyrotropin in hyperthyroidism [Letter]. Clin Chem 1983;29:1688. 15. Rodbard D. Statistical estimation of the minimal detectable concentration (“sensitivity”) for radioligand assays. Anal Biochem 1978;90:1-12. 16. Cornbleet PJ, Gochman N. Incorrect least-squares regression coefficients in method-comparison analysis. Clin Chem 1979;25:432-8. 17. SiegelS. Estadistica no parametrica aplicada a las ciencias de la conducta. Mexico City:Editorial Trillas, S. A. , 1982:143-55. 18. Durham AP. The upper limit ofnormal for thyrotropin is 3 or 4 milli-int. unitsfL. Clin Chem 1985;31:296-8. 19. BassetF,Eastman CJ, Ma G,Maberly GF, Smith HC. Diagnostic value of thyrotropin concentrations in serum as measured by a
Electronic diary assessment of the temporal association between proximal anger and intimate partner violence perpetration

Objective: The temporal association between proximal anger and intimate partner violence (IPV) perpetration was examined using electronic daily diary assessment methodology. In addition, relevant demographic and relational variables were examined as potential moderators of the association between anger and IPV perpetration. Method: Participants were 184 men and women in dating relationships who completed daily surveys reporting anger and IPV for a period of 2 months. Results: Increases in proximal anger were associated with greater odds of psychological (2.78), physical (2.38), and sexual aggression perpetration (2.27). Analyses also demonstrated a quadratic relationship for psychological aggression and proximal anger, such that increased anger was more strongly associated with psychological aggression when anger was relatively low versus higher in the first place. Moderators of the relationship between proximal anger and IPV perpetration included age and length of relationship. Conclusions: These data are the first to provide evidence for the temporal relationship between anger and IPV perpetration. Data support electronic diary assessment as an effective way to assess moment-to-moment partner interactions, increase participant compliance, and minimize retrospective recall bias. Electronic diary assessment may also hold promise for the creation of intervention programs that address proximal anger in conjunction with distal variables that increase risk for IPV perpetration.
Linear Propagation Effects in Mode-Division Multiplexing Systems

In this paper, we review linear propagation effects in a multimode fiber (MMF) and their impact on performance and complexity in long-haul mode-division multiplexing (MDM) systems. We highlight the many similarities to wireless multi-input multioutput (MIMO) systems. Mode-dependent loss and gain (MDL), analogous to multipath fading, can reduce average channel capacity and cause outage in narrowband systems. Modal dispersion (MD), analogous to multipath delay spread, affects the complexity of MIMO equalization, but has no fundamental effect on performance. Optimal MIMO transmission uses a basis of the Schmidt modes, which may be obtained by a singular value decomposition of the MIMO channel. In the special case of a unitary channel (no MDL), an optimal basis is the set of principal modes, which are eigenvectors of a group delay operator, and are free of signal distortion to first order. We present a concatenation rule for the accumulation of MD along a multisection link. We review mode coupling in MMF, including physical origins, models, and regimes of weak and strong coupling. Strong mode coupling is a key to overcoming challenges in MDM systems. Strong coupling reduces the group delay spread from MD, minimizing the complexity of MIMO signal processing. Likewise, it reduces the variations of loss and gain from MDL, maximizing channel capacity. In the strong-coupling regime, the statistics of MD and MDL depend only on the number of modes and the variance of accumulated group delay or loss/gain, and can be derived from the eigenvalue distributions of certain Gaussian random matrices.
DltABCD- and MprF-Mediated Cell Envelope Modifications of Staphylococcus aureus Confer Resistance to Platelet Microbicidal Proteins and Contribute to Virulence in a Rabbit Endocarditis Model

ABSTRACT The DltABCD and MprF proteins contribute a net positive charge to the Staphylococcus aureus surface envelope by alanylating and lysinylating teichoic acids and membrane phosphatidylglycerol, respectively. These surface charge modifications are associated with increased in vitro resistance profiles of S. aureus to a number of endogenous cationic antimicrobial peptides (CAPs), such as α-defensins. The current study investigated the effects of dltA and mprF mutations on the following host factors relevant to endovascular infections: (i) in vitro susceptibility to the CAP thrombin-induced platelet microbicidal protein 1 (tPMP-1), (ii) in vitro adherence to endothelial cells (EC) and matrix proteins, and (iii) in vivo virulence in an endovascular infection model (rabbit endocarditis) in which tPMP-1 is felt to play a role in limiting S. aureus pathogenesis. Both mutations resulted in substantial increases in the in vitro susceptibility to tPMP-1 compared to that of the parental strain. The dltA (but not the mprF) mutation resulted in a significantly reduced capacity to bind to EC in vitro, while neither mutation adversely impacted in vitro binding to fibronectin, fibrinogen, or platelets. In vivo, both mutations significantly attenuated virulence in terms of early colonization of sterile vegetations and subsequent proliferation at this site (versus the parental strain). However, only the dltA mutation significantly reduced metastatic infections in kidneys and spleens compared to those in animals infected with the parental strain. These data underscore the importance of resistance to distinct CAPs and of teichoic acid-dependent EC interactions in the context of endovascular infection pathogenesis.
Helicobacter pylori infection in children: relation with current household living conditions.

BACKGROUND: Studies demonstrating that deprived household living conditions during childhood are risk factors for acquisition of Helicobacter pylori infection have been performed mainly in adults, who probably acquired the infection several decades ago. This study investigates whether deprived household living conditions remain important risk factors for infection in subjects (children) with recently acquired infection. AIMS: To examine the relation between current household living conditions and acquisition of H pylori infection in childhood. SUBJECTS/SETTING: Opportunistically recruited group of 367 children, aged 3 to 15 years, undergoing routine non-gastrointestinal day surgery. METHODS: Anti-H pylori IgG antibodies measured by a commercial enzyme linked immunosorbent assay validated for use in children. Postal questionnaire collecting sociodemographic data and data on household living conditions. RESULTS: Infection was associated with social class and overcrowding in the household. After adjustment for age, social class, and household density, a positive association remained between infection with H pylori and bed-sharing between children and parents on one or two nights per week, odds ratio for infection (95% CI), 2.29 (1.21, 4.32) or more frequently, odds ratio for infection (95% CI), 2.95 (1.35, 6.45). CONCLUSIONS: The continuing importance of household living conditions in the acquisition of H pylori infection is confirmed and household crowding and sharing a bed with a parent are identified as risk factors for infection.
Phonological awareness intervention for verbal working memory skills in school-age children with specific language impairment and concomitant word reading difficulties

This exploratory investigation examined the effects of explicit phonological awareness intervention on each subcomponent of Baddeley’s verbal working memory model. Fifty school-age children with specific language impairment (SLI) and concurrent deficits in word reading were randomly assigned to either an experimental (n=25) or a control group (n = 25). Children in both groups received individual traditional language intervention for four, 1 hour sessions each week for 4 weeks (16 hrs). The experimental group received an additional 20 min of phonological awareness intervention each day (5.3 hrs). Participants in the experimental group significantly outperformed the children in the control group across all verbal working memory measures. The strongest effects were found for the digit recall and word list recall subtests, which were used to assess the verbal short-term memory component of the model (i.e., phonological loop). The next largest effect sizes were reported for the subtests of the verbal working memory functioning (i.e., phonological loop and central executive). The smallest change was found on the recalling sentences subtest, which was chosen to represent the episodic buffer component. These results suggest that school-age children with SLI and concomitant word-reading difficulties in second through third grade who receive explicit phonological awareness intervention can make significant gains on untrained verbal working memory skills in a relatively short period of time which underscores the importance of phonological awareness intervention beyond first grade.
The Importance of the Home Literacy Environment for Developing Literacy Skills in Young Children Who Are Deaf or Hard of Hearing

Four-year-old Olivia, who is deaf and has used a cochlear implant for 3 years, and her mother, Laura, enjoy looking at storybooks together at home. During storybook reading, Laura notices that Olivia tries to imitate some words that she reads to her yet does not seem to understand many words or story events. Laura is unsure whether to stop the storybook interaction and encourage Olivia’s attempts at word imitation and directly teach her the new vocabulary or continue reading the story for pleasure. Olivia’s preschool teacher, Ms. Lynn, also notices that Olivia displays a keen interest in storybook reading but is not able to answer or ask simple questions about the story. What can Laura and Ms. Lynn do collaboratively to support Olivia’s emerging language and literacy skills to better prepare Olivia for her upcoming transition into kindergarten?
Transactions Papers - Device Placement for Heterogeneous Wireless Sensor Networks: Minimum Cost with Lifetime Constraints

Device placement is a fundamental factor in determining the coverage, connectivity, cost and lifetime of a wireless sensor network (WSN). In this paper, we explore the problem of relay node placement in heterogeneous WSN. We formulate a generalized node placement optimization problem aimed at minimizing the network cost with constraints on lifetime and connectivity. Depending on the constraints, two representative scenarios of this problem are described. We characterize the first problem, where relay nodes are not energy constrained, as a minimum set covering problem. We further consider a more challenging scenario, where all nodes are energy limited. As an optimal solution to this problem is difficult to obtain, a two-phase approach is proposed, in which locally optimal design decisions are taken. The placement of the first phase relay nodes (FPRN), which are directly connected to sensor nodes (SN), is modeled as a minimum set covering problem. To ensure the relaying of the traffic from the FPRN to the base station, three heuristic schemes are proposed to place the second phase relay nodes (SPRN). Furthermore, a lower bound on the minimum number of SPRN required for connectivity is provided. The efficiency of our proposals is investigated by numerical examples.
Factors Associated with Biochemical Remission after Microscopic Transsphenoidal Surgery for Acromegaly

Abstract Objectives To analyze surgical outcomes and predictive factors of disease remission in acromegaly patients who underwent microscopic transsphenoidal surgery (TSS) for a growth hormone (GH)-secreting adenoma. Design A 6-year retrospective review of 86 consecutive acromegaly surgeries. Setting Procedures performed at a single institution by a single surgeon. Participants Seventy acromegaly patients. Main Outcome Measures Demographic information, preoperative laboratory values, tumor imaging data, and morphological and immunohistochemical data were collected. Predictive values using the latest and most stringent biochemical remission criteria were determined using univariate and multivariate statistical analyses. Results Remission rate for 59 (18 males) acromegaly patients meeting the study inclusion criteria was 52.5%. Remission rates for micro- and macroadenomas were 81.8% and 45.8%, respectively. Patients of older age, with a smaller tumor, lower Knosp grade, lower preoperative GH, and insulinlike growth factor 1 levels were more likely to achieve remission. Remission rate decreased significantly with repeat surgeries. Those patients with adenomas that stained positive for somatostatin receptor subtype 2A were less likely to experience tumor recurrence and more likely to respond to medical treatment with persistent or elevated GH hypersecretion. Conclusions Microscopic TSS continues to be a viable means for treating acromegaly patients. Patients should be followed long term.
Emotional Nouns Affect Attachment Decisions in Sentence Completion Tasks

We report three sentence completion experiments in which we manipulate the emotional dimension of the nouns in a complex noun phrase (NP) that precedes a relative clause (RC), as in the classic ambiguity in Someone shot the servant of the actress who was on the balcony. The aim was to see whether nouns such as orgy or genocide affect the well-established preference of Spanish to adjoin the relative clause high in the tree (to servant instead of actress in the example above). We manipulated the valence and arousal of the lexical entities residing in the NP. Our results indicate that (a) the inclusion of either pleasant or unpleasant words induces changes in the usual NP1 preference found in Spanish; (b) the effects of high-arousal words are especially clear, in that they pull RC adjunction towards the NP where they are located, be it the NP1 or the NP2; and (c) in the context of sentence production, these kinds of words seem intense enough to promote changes in (and even reverse) a solid syntactic bias. We discuss these findings in the light of existing theories of syntactic ambiguity resolution.
From Teachers for Education Change

This article concerns issues connected to social inclusion and exclusion in Swedish upper secondary education in the late 1990s, after the 1994 curriculum reform. It is based on ethnographic fieldwork that was completed in 2000 and that appropriated participant observation and interview methods in data production. Extensive comments from teachers and headteachers on strategies and ideas about how to work within the context of the reform, with respect particularly to expressed aims of providing an education of good and comparable quality for all students and opening up higher education to groups who were previously excluded from it, figure strongly in the article. The intention was to discover what the people working within schools felt about their working experiences with respect to social inclusion and to understand what stands in the way of the realisation of social inclusion at present. The article indicates that two competing regimes of truth currently operate on what counts as valid teacher work but also that a creative subject position for all education participants is important in projects of inclusion.
Regional Quality Assurance Activity in Higher Education in Southeast Asia: Its Characteristics and Driving Forces

Abstract This article analyses the characteristics and driving forces of regional quality assurance activity in Southeast Asia, which has been actively promoted in recent years by the ASEAN University Network, an organisation for higher education under the auspices of the Association of Southeast Asian Nations (ASEAN). There are now more collaborative efforts among quality assurance agencies and higher education institutions for quality assurance at the international and regional levels as ‘globalisation’ of higher education accelerates. However, each effort is formed with different objectives and characteristics, as it is driven by different forces. In Southeast Asia, ASEAN’s policies, such as reducing the economic gap within the region and creation of a single market, and member countries’ policies to internationalise higher education institutions have brought about a strong need to improve the quality of higher education and to foster harmonisation of higher education systems, which have been the driving force behind this regional quality assurance activity.
Magnetic Cryopreservation for Dental Pulp Stem Cells

Magnetic cryopreservation has been successfully used for tooth banking with satisfactory implantation outcomes, suggesting that the method preserves human periodontal ligament cells and dental pulp stem cells (DPSCs). Therefore, magnetic cryopreservation may be applied for the preservation of DPSCs; however, this method has not been evaluated yet. A reliable cryopreservation method for live-cell preservation is important for the clinical applications of regenerative medicine. The conventional slow-freezing procedure with 10% dimethylsulfoxide (DMSO) may not be appropriate for stem cell-based therapies because DMSO is cytotoxic. The objective of this study was to investigate whether magnetic cryopreservation can be applied for DPSC cryopreservation. Cells isolated from human dental pulp were subjected to magnetic cryopreservation. Postthawing cell viability, adhesion, proliferation, expression of markers for mesenchymal stem cells (MSCs), differentiation ability of magnetically cryopreserved DPSCs and DNA stability were compared to those of cells subjected to the conventional slow-freezing method. The results indicated that a serum-free cryopreservation medium (SFM) containing 3% DMSO is optimal for magnetic cryopreservation. Post-thaw magnetically cryopreserved DPSCs express MSC markers, and perform osteogenesis and adipogenesis after induction similarly to fresh MSCs. No significant DNA damage was found in magnetically cryopreserved DPSCs. Magnetic cryopreservation is thus a reliable and effective method for storage of DPSCs. The smaller amount of DMSO required in SFM for cryopreservation is beneficial for the clinical applications of post-thaw cells in regenerative medicine.
Learning the correlational structure of stimuli in a one-attribute classification task

In category learning experiments, participants typically do not learn within-category correlations unless the composition of the categories or the task demands compel them to do so. To determine if correlations among attributes could be learned without explicitly focusing the participants’ attention on them, a task was designed that allowed stimuli to be classified on the basis of a single perfectly predictive attribute. Each training stimulus also included attributes that were either perfectly or partly correlated with the rule attribute. Then, in a test phase, the impact of eliminating the rule attribute on classification was evaluated. The experiment showed that some of the attributes that were perfectly correlated with the rule attribute were learned. These attributes could be used to classify the test exemplars even though the rule attribute had been removed. This experiment provides evidence that within-category correlations can be learned incidentally during classification tasks.