Miracle-dz/UniFAR-FTUs huggingface dataset:
Old World Arenaviruses Enter the Host Cell via the Multivesicular Body and Depend on the Endosomal Sorting Complex Required for Transport

The highly pathogenic Old World arenavirus Lassa virus (LASV) and the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) use α-dystroglycan as a cellular receptor and enter the host cell by an unusual endocytotic pathway independent of clathrin, caveolin, dynamin, and actin. Upon internalization, the viruses are delivered to acidified endosomes in a Rab5-independent manner bypassing classical routes of incoming vesicular trafficking. Here we sought to identify cellular factors involved in the unusual and largely unknown entry pathway of LASV and LCMV. Cell entry of LASV and LCMV required microtubular transport to late endosomes, consistent with the low fusion pH of the viral envelope glycoproteins. Productive infection with recombinant LCMV expressing LASV envelope glycoprotein (rLCMV-LASVGP) and LCMV depended on phosphatidyl inositol 3-kinase (PI3K) as well as lysobisphosphatidic acid (LBPA), an unusual phospholipid that is involved in the formation of intraluminal vesicles (ILV) of the multivesicular body (MVB) of the late endosome. We provide evidence for a role of the endosomal sorting complex required for transport (ESCRT) in LASV and LCMV cell entry, in particular the ESCRT components Hrs, Tsg101, Vps22, and Vps24, as well as the ESCRT-associated ATPase Vps4 involved in fission of ILV. Productive infection with rLCMV-LASVGP and LCMV also critically depended on the ESCRT-associated protein Alix, which is implicated in membrane dynamics of the MVB/late endosomes. Our study identifies crucial cellular factors implicated in Old World arenavirus cell entry and indicates that LASV and LCMV invade the host cell passing via the MVB/late endosome. Our data further suggest that the virus-receptor complexes undergo sorting into ILV of the MVB mediated by the ESCRT, possibly using a pathway that may be linked to the cellular trafficking and degradation of the cellular receptor.
CD95 death‐inducing signaling complex formation and internalization occur in lipid rafts of type I and type II cells

We investigated the membrane localization of CD95 in type I and type II cells, which differ in their ability to recruit and activate caspase‐8. We found that CD95 was preferentially located inlipid rafts of type I cells, while it was present both in raft and non‐raft plasma membrane sub‐domains of type II cells. After stimulation, CD95 located in phospholipid‐rich plasma membrane was recruited to lipid rafts in both types of cells. Similarly, CD95 cross‐linking resulted in caspase‐independent translocation of FADD/MORT1 and caspase‐8 to the lipid rafts, which was prevented by a death domain‐defective receptor. CD95 internalization was then rapid in type I and delayed in type II cells and showed a substantial correlation with the kinetics of Fas‐associated death domain (FADD)and caspase‐8 recruitment to lipid rafts. Finally, electron microscopy analysis showed that after CD95 stimulation lipid rafts aggregated in large clusters that were internalized in endosomal vesicles, where caspase‐8 underwent massive processing. Taken together, our data demonstrate that CD95 death‐inducing signaling complex formation and internalization in type I and type II cells occur in lipidrafts, which are a major site of caspase‐8 activation.
Brazilian Children's Conceptions of Peace, War, and Violence

This study investigates the understanding of peace, war, and violence of children in southern Brazil. Sixty-one younger children (30 boys and 31 girls; M age 7.6; SD =. 82) and 63 older children (29 boys and 34 girls; M age 12.7; SD =. 72) responded to an interview about either peace and violence or war and violence. The two interviews were equally distributed among participants according to age and sex. Data were examined for age and gender differences through content analysis and chi-square tests. In their responses, younger children accentuated concrete aspects of violence, such as weapons, while older children emphasized humane attitudes. Older boys highlighted conflicting interests; older girls emphasized a sense of human well-being. Results of this study are compared to those of similar international studies and the implications for peace education are discussed in the context of modern Brazilian culture and global trends.
Facilitating Commenting and Requesting Skills in 3-Year-Old Children With Disabilities

The current study evaluated the commenting behaviors and verbal and non-verbal requests of children with disabilities (e.g., developmental delays, specific language impairment) who participated in a shared storybook social communication intervention that included sociodramatic play themes and targeted skills such as initiations, responses, name use, proximity, and turn-taking. Six children who enrolled in a self-contained classroom met the inclusion criteria. A multiple-baseline design was used to determine the effects of the intervention. The social communication intervention was highly effective for all children in increasing the rate of commenting behaviors and for verbal behavior from baseline to intervention. Several implications for practice were derived from the findings. By teaching children social communication strategies, the quality of social interactions that children have with their peers is likely to improve. The intervention offers a more systematic technique for teaching social communication and play skills than do informal strategies commonly used by teachers. Social validity assessments indicated that teachers found the intervention acceptable and produced important changes in behavior.
Identification of Rice Transcription Factors Associated with Drought Tolerance Using the Ecotilling Method

The drought tolerance (DT) of plants is a complex quantitative trait. Under natural and artificial selection, drought tolerance represents the crop survival ability and production capacity under drought conditions (Luo, 2010). To understand the regulation mechanism of varied drought tolerance among rice genotypes, 95 diverse rice landraces or varieties were evaluated within a field screen facility based on the ‘line–source soil moisture gradient’, and their resistance varied from extremely resistant to sensitive. The method of Ecotype Targeting Induced Local Lesions in Genomes (Ecotilling) was used to analyze the diversity in the promoters of 24 transcription factor families. The bands separated by electrophoresis using Ecotilling were converted into molecular markers. STRUCTURE analysis revealed a value of K = 2, namely, the population with two subgroups (i.e., indica and japonica), which coincided very well with the UPGMA clusters (NTSYS-pc software) using distance-based analysis and InDel markers. Then the association analysis between the promoter diversity of these transcription factors and the DT index/level of each variety was performed. The results showed that three genes were associated with the DT index and that five genes were associated with the DT level. The sequences of these associated genes are complex and variable, especially at approximately 1000 bp upstream of the transcription initiation sites. The study illuminated that association analysis aimed at Ecotilling diversity of natural groups could facilitate the isolation of rice genes related to complex quantitative traits.
Body-Esteem Mediates The Relationship Between Self-Esteem and Social Anxiety: The Moderating Roles of Weight and Gender

ABSTRACT The prevalence of social anxiety during adolescence is high, and it is necessary that we increase our knowledge on the related factors that contribute to social anxiety. The present study sought to examine the relationships among self-esteem, body-esteem, and social anxiety among adolescent students, as well as to examine the mediating role of body-esteem between self-esteem and social anxiety, and to examine the moderating roles of weight and gender between body-esteem and self-esteem with social anxiety. The participants comprised 520 high-school students from eight high schools in Tehran city in District 11 of the Ministry of Education in 2012/13. Structural equation modelling showed that adolescents with poor self-esteem and poor body-esteem were more likely to show social anxiety. Body-esteem partially mediated between self-esteem and social anxiety. Weight and gender moderated between body-esteem and self-esteem with social anxiety. Our results highlight the roles of body-esteem and self-esteem as promising avenues for reducing social anxiety among adolescent students.
A retrospective analysis of 44 implants with no rotational primary stability used for fixed prosthesis anchorage.

PURPOSE The aim of this study was to determine the osseointegration potential of implants with apical primary stability but no resistance to rotation in variable clinical conditions. MATERIALS AND METHODS Patient records of treatment performed between October 1993 and May 2004 were reviewed for primary implant stability. Patients who exhibited implants without rotational primary stability (RPS) were reviewed further to determine patient age, gender, implant type, implant surface, loading protocol, and prosthesis type. RESULTS Forty-four implants without RPS were reported in 12.8% of patients treated during the period reviewed. Statistical significant differences in cumulative survival rate (CSR) were seen with implant location and surface. No significant difference in CSR was observed in relation to loading protocol, despite a higher success rate (17.4% higher) for the immediate loading population. No difference in CSR was observed with respect to gender. DISCUSSION AND CONCLUSION In the early years of machined implants, there was a higher failure rate of implants without RPS. The results for this implant population show that there are statistically significant differences in survival rate between the maxilla and mandible, and also between titanium oxide-surfaced implants and machined implants. The sample size of this study limited the statistical significance of the difference in CSR observed between loading protocols. Implants without RPS that are not removed at the time of surgery can become osseointegrated with a survival rate of 82%. Survival rates increase when testing sample is limited to titanium oxide-surfaced implants under an immediate loading protocol. Under the appropriate level of risk, implants that have apical primary stability but no resistance to rotation can become osseointegrated when incorporated in a rigid prosthesis under immediate loading circumstances.
The Reversed Role of Magnets in St. Louis

Magnet schools were originally created to attract a diverse student population. Using data from the 23 magnet schools in St. Louis, this longitudinal study is twofold: first, to review the performance outcomes of the magnet schools across a 5-year period, between 2005-2006 and 2009-2010, and second, to examine whether the magnet schools are fostering segregation among the city’s Black families. The relationship between a school’s demographics, its surrounding community, and student outcomes is investigated. Finally, these data are used to question whether student demographics should matter for Black students if appropriate academic outcomes are being met.
Spectral Dynamics and Therapeutic Implications of the Theta/Alpha Crossover in Alpha-Theta Neurofeedback

The theta-over-alpha frequency crossover seen in alpha-theta neurofeedback, considered an important factor in the treatment's success, has had little definitive research. This study examined 182 alpha-theta session graphs from 10 subject case files for interactions between frequency band activity and subject reports of imagery or biographical memories during crossovers, as well as treatment outcomes. Statistical analyses revealed significantly more reports of imagery/memories during crossover conditions having specific spectral, amplitude, and duration characteristics. Imagery reports were more likely to occur during crossover activity of 1 microvolt or more, lasting at least 3 minutes, and including 3. 75µv of 15–20 Hz beta. This defined therapeutic crossover condition also was significantly related to better treatment outcome measures.
Outcomes in right liver lobe transplantation: a matched pair analysis

Split liver transplantation (SLT) has proven to be an effective technique of increasing the donor pool and thereby reducing adult and paediatric waiting list mortality. There remains concern regarding complications in adult recipients. Here, we compare SLT with matched whole liver grafts. Adult recipients of primary extended right lobe grafts (ERL) were matched to recipients of whole liver transplantations (WLTs) according to the following criteria: model of end‐stage liver disease (MELD) score, recipient age, indication for liver transplantation and year of transplantation. Twenty‐seven pairs of recipients were transplanted for chronic liver disease. The overall 30‐day patient survival rates after ERL and WLT were 88.9% and 92.5% and 3‐year survival rates after SLT and WLT were 77.8% and 85.2% respectively (log‐rank = 0.38). Two patients with SLTs had hepatic artery thromboses and were retransplanted with none from the WLT group. The prevalence of a biliary leak was higher among the SLT group (n = 4) compared with none in the WLT group (P = 0.05). Patients with preoperative hyponatraemia showed a trend towards poorer survival after SLT compared with WLT. Our data suggest that SLT with extended right liver lobes, although not significantly different, shows a trend towards a poorer outcome.
Lack of evidence for diaphragmatic fatigue over the course of the night in obstructive sleep apnoea.

The aim of this study was to determine whether diaphragmatic fatigue develops over the course of the night in patients with obstructive sleep apnoea (OSA). Patients with severe OSA underwent overnight polysomnography with the addition of gastric and oesophageal catheters for measurement of transdiaphragmatic pressure (Pdi) (n = 7) and a gastro-oesophageal electrode for determination of diaphragmatic electromyogram (EMGdi) (n = 5). Analyses of Pdi and EMGdi were performed to detect fatigue during the large inspiratory efforts at the end of apnoeas in Stage 2 sleep at the beginning and end of the night. Measurements included Pdi values, shape analysis of the Pdi waveform, the relaxation rate (tau R) of Pdi, EMGdi and its relationship to Pdi, and the centroid frequency (fc) of EMGdi. End of apnoeic Pdi and EMGdi increased from the beginning to end of the night (e.g. 19 +/- 14% increase in Pdi; p < 0.05). The rate of increase in Pdi and EMGdi during apnoeas did not change. The Pdi versus EMGdi relationship was linear, and remained unchanged over the course of the night. There was no significant change in the shape of the Pdi waveform, and there were no changes in tau R from the beginning to the end of the night (0.13 +/- 0.01 s for both periods). There was also no shift in the fc of the EMGdi power spectrum (94 +/- 5 vs 93 +/- 6 Hz; NS), and no change in the relationship of fc to Pdi or EMGdi from the beginning to the end of the night. These findings do not support the development of diaphragmatic fatigue over the course of the night in obstructive sleep apnoea.
Second Harmonics Effects in Random Duffing Oscillators

We consider a stochastic model for Duffing oscillators, where the nonlinearity and the randomness are scaled in such a way that they interact strongly. A typical feature is the appearance of second harmonics effects. An asymptotic statistical analysis for these oscillators is performed in the diffusion limit, when a suitable absorbing boundary condition is imposed, according to the underlying physical problem. The related Fokker-Planck equation has been numerically solved to obtain the first two moments of the oscillator's displacement from its rest-position. Dependence on the nonlinearity strength and on the location of the absorbing boundary has also been investigated. Such results have been compared with those computed solving the corresponding stochastic Ito differential equations by a Monte Carlo method, where quasi-random sequences of numbers have been efficiently used.
A Data-adaptive and Dynamic Segmentation Index for Whole Matching on Time Series

Similarity search on time series is an essential operation in many applications. In the state-of-the-art methods, such as the R-tree based methods, SAX and iSAX, time series are by default divided into equi-length segments globally, that is, all time series are segmented in the same way. Those methods then focus on how to approximate or symbolize the segments and construct indexes. In this paper, we make an important observation: global segmentation of all time series may incur unnecessary cost in space and time for indexing time series. We develop DSTree, a data adaptive and dynamic segmentation index on time series. In addition to savings in space and time, our new index can provide tight upper and lower bounds on distances between time series. An extensive empirical study shows that our new index DSTree supports time series similarity search effectively and efficiently.
ARE PATIENTS WHO ARE INTOLERANT TO ONE SSRI, INTOLERANT TO ANOTHER?

One hundred patients with major depression who had discontinued fluoxetine because of side effects were enrolled in a multicenter, open, 8-week study of sertraline. After a washout period of at least 3 weeks following fluoxetine discontinuation and an additional 1-week, single-blind, placebo washout period, patients began treatment with 50 mg sertraline once daily. Based on the clinician's judgment of patient response, doses were titrated upward if necessary. The maximum daily dose of sertraline was 200 mg. Depressive symptoms and adverse events were assessed weekly. An interim analysis was conducted of the first 93 patients who completed the study. Of 91 evaluable patients, 69 sertraline-treated patients (75.8%) experienced significant improvement in depression. Only 8 of 93 patients (8.6%) discontinued sertraline because of side effects.
Organizations and supervisors as sources of support and targets of commitment: a longitudinal study

The main objective of this study was to examine the relationship between perceived support and affective commitment, and the linkages between these constructs and some of their common antecedents and consequences. More precisely, using a sample of 238 employees, we conducted a longitudinal study to examine the linkages between the favorableness of intrinsically and extrinsically satisfying job conditions, perceived organizational support, perceived supervisor support, affective commitment to the organization and supervisor, and turnover. Affective commitment to the supervisor was found to completely mediate the effect of perceived supervisor support on turnover, whereas neither perceived organizational support nor organizational affective commitment were significantly related to turnover. Perceived organizational support partially mediated the effect of favorable intrinsically satisfying job conditions on organizational affective commitment and fully mediated the effect of extrinsically satisfying job conditions on organizational affective commitment. Finally, perceived supervisor support totally mediated the effect of favorable intrinsically satisfying job conditions on affective commitment to the supervisor. As a whole, findings suggest that exchange relationships between employees and their supervisors should be further investigated in future turnover research. Copyright © 2003 John Wiley & Sons, Ltd.
Magnetic resonance imaging and mammography in women with a hereditary risk of breast cancer.

BACKGROUND Although breast cancer screening is recommended to start at a younger age for women with a hereditary risk of breast cancer, the sensitivity of mammography for these women is reduced. We compared magnetic resonance imaging (MRI) with mammography to determine which is more sensitive and whether MRI could play a role in the early detection of breast cancer for these women. METHODS We constructed a retrospective cohort of all breast MRI and mammography surveillance reports made in our department from November 1994 to February 2001. All of the 179 women in the cohort had received biannual palpation in addition to annual imaging by MRI, mammography, or both. The 258 MRI images and the 262 mammograms were classified with the use of the BI-RADS (i.e., Breast Imaging Reporting and Data System) scoring system, which has five categories to indicate the level of suspicion of a lesion. Receiver operator characteristic curves were generated for MRI and mammography, and the area under each curve (AUC) was assessed for the entire cohort of 179 women and for a subset of 75 women who had received both an MRI and a mammographic examination within a 4-month period. All statistical tests were two-sided. RESULTS In the cohort of 179 women, we detected 13 breast cancers. Seven cancers were not revealed by mammography, but all were detected by MRI. For the entire cohort, the AUC for mammography was 0.74 (95% confidence interval [CI] = 0.68 to 0.79), and the AUC for MRI was 0.99 (95% CI = 0.98 to 1.0). For the subset of women who had both examinations, the AUC for mammography was 0.70 (95% CI = 0.60 to 0.80), and the AUC for MRI was 0.98 (95% CI = 0.95 to 1.0). CONCLUSION MRI was more accurate than mammography in annual breast cancer surveillance of women with a hereditary risk of breast cancer. Larger prospective studies to examine the role of MRI in screening programs are justified.
Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit

Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.