Miracle-dz/UniFAR-FTUs huggingface dataset:
Real-time in vivo imaging of size-dependent transport and toxicity of gold nanoparticles in zebrafish embryos using single nanoparticle plasmonic spectroscopy

Noble metal nanoparticles (NPs) show distinctive plasmonic optical properties and superior photostability, enabling them to serve as photostable multi-coloured optical molecular probes and sensors for real-time in vivo imaging. To effectively study biological functions in vivo, it is essential that the NP probes are biocompatible and can be delivered into living organisms non-invasively. In this study, we have synthesized, purified and characterized stable (non-aggregated) gold (Au) NPs (86.2 ± 10.8 nm). We have developed dark-field single NP plasmonic microscopy and spectroscopy to study their transport into early developing zebrafish embryos (cleavage stage) and their effects on embryonic development in real-time at single NP resolution. We found that single Au NPs (75–97 nm) passively diffused into the embryos via their chorionic pore canals, and stayed inside the embryos throughout their entire development (120 h). The majority of embryos (96 ± 3%) that were chronically incubated with the Au NPs (0–20 pM) for 120 h developed to normal zebrafish, while an insignificant percentage of embryos developed to deformed zebrafish (1 ± 1)% or dead (3 ± 3)%. Interestingly, we did not observe dose-dependent effects of the Au NPs (0–20 pM) on embryonic development. By comparing with our previous studies of smaller Au NPs (11.6 ± 0.9 nm) and similar-sized Ag NPs (95.4 ± 16.0 nm), we found that the larger Au NPs are more biocompatible than the smaller Au NPs, while the similar-sized Ag NPs are much more toxic than Au NPs. This study offers in vivo assays and single NP microscopy and spectroscopy to characterize the biocompatibility and toxicity of single NPs, and new insights into the rational design of more biocompatible plasmonic NP imaging probes.
Effects of long-term changes in forest canopy structure on rainfall interception loss

Forest plays a key role in spatial distribution of rainfall and nutrients at fine spatial scales. Areas of localized rainfall and nutrient input at the soil surface may have a large effect on several hydrological and bio-geochemical processes. In this chapter, a Douglas-fir stand was revisited to evaluate the changes in throughfall spatial distribution and its temporal stability due to forest growth and thinning. We used 32 funnel-type collectors distributed in a random stratified array (320 locations) within a 0.2 ha plot to measure throughfall amounts from February to November 2015. The throughfall variability was much lower as compared to the values reported ~25 years ago in the same site. We further assessed the spatial patterns of throughfall in spring and summer. We detected a spatial correlation length of 12 m and 8 m for spring and summer, respectively, which are higher than the values reported for other mature Douglas-fir forests in similar climatic conditions. Temporal stability plots confirmed that detected spatial patterns were stable in time.
Nursing Care Interpersonal Relationship Questionnaire: elaboration and validation 1

ABSTRACT Objective: to elaborate an instrument for the measurement of the interpersonal relationship in nursing care through the Item Response Theory, and the validation thereof. Method: methodological study, which followed the three poles of psychometry: theoretical, empirical and analytical. The Nursing Care Interpersonal Relationship Questionnaire was developed in light of the Imogene King’s Interpersonal Conceptual Model and the psychometric properties were studied through the Item Response Theory in a sample of 950 patients attended in Primary, Secondary and Tertiary Health Care. Results: the final instrument consisted of 31 items, with Cronbach’s alpha of 0.90 and McDonald’s Omega of 0.92. The parameters of the Item Response Theory demonstrated high discrimination in 28 items, being developed a five-level interpretive scale. At the first level, the communication process begins, gaining a wealth of interaction. Subsequent levels demonstrate qualitatively the points of effectiveness of the interpersonal relationship with the involvement of behaviors related to the concepts of transaction and interaction, followed by the concept of role. Conclusion: the instrument was created and proved to be consistent to measure interpersonal relationship in nursing care, as it presented adequate reliability and validity parameters.
Opioids and the immune system – friend or foe

Systemically administered opioids are among the most powerful analgesics for treating severe pain. Several negative side effects (respiratory depression, addiction, nausea and confusion) and the risk of opioid‐induced hyperalgesia accompany opioid administration. One other side effect is the potential of opioids to suppress the immune response and thereby to increase the vulnerability to infections. The link between opioids and immunosuppression has been investigated both in vitro and in vivo as well as in patients. However, the results are inconsistent: Exogenous opioids such as morphine and fentanyl have been found to impair the function of macrophages, natural killer cells and T‐cells and to weaken the gut barrier in vitro and in animal studies. In epidemiological studies, high doses and the initiation of opioid therapy for non‐malignant pain have been correlated with a higher risk of infectious diseases such as pneumonia. However clear randomized controlled studies are missing. Furthermore, immune cells including neutrophils, macrophages and T‐cells have been shown to secrete endogenous opioid peptides, which then bind to peripheral opioid receptors to relieve inflammatory and neuropathic pain. In addition to cytokines, hormones and bacterial products, the release of opioid peptides is stimulated by the application of exogenous opioids. In summary, there is a reciprocal interaction between the immune system and endogenous as well as exogenous opioids. Further to the existing epidemiological studies, controlled clinical studies are needed in the future to elucidate the role of the opioid–immune system interaction in patients and to determine its clinical relevance.
When Heavy-Tailed and Light-Tailed Flows Compete: The Response Time Tail Under Generalized Max-Weight Scheduling

This paper focuses on the design and analysis of scheduling policies for multi-class queues, such as those found in wireless networks and high-speed switches. In this context, we study the response-time tail under generalized max-weight policies in settings where the traffic flows are highly asymmetric. Specifically, we consider a setting where a bursty flow, modeled using heavy-tailed statistics, competes with a more benign, light-tailed flow. In this setting, we prove that classical max-weight scheduling, which is known to be throughput optimal, results in the light-tailed flow having heavy-tailed response times. However, we show that via a careful design of inter-queue scheduling policy (from the class of generalized max-weight policies) and intra-queue scheduling policies, it is possible to maintain throughput optimality, and guarantee light-tailed delays for the light-tailed flow, without affecting the response-time tail for the heavy-tailed flow.
Perindopril attenuates tubular hypoxia and inflammation in an experimental model of diabetic nephropathy in transgenic Ren‐2 rats

Background: Renal hypoxia plays a role in the development of diabetic nephropathy, and may be mediated by overactivity of the renin‐angiotensin‐aldosterone system (RAAS). In this study the localization of cellular hypoxia in an experimental model of diabetic nephropathy was assessed, and the effect of the angiotensin‐converting enzyme inhibitor perindopril on hypoxia evaluated.
Tubular aggregate myopathy caused by a novel mutation in the cytoplasmic domain of STIM1

Objective: To identify the gene mutation of tubular aggregate myopathy (TAM) and gain mechanistic insight into the pathogenesis of the disorder. Methods: We described a family affected by autosomal dominant TAM and performed exome and Sanger sequencing to identify mutations. We further analyzed the functional significance of the identified mutation by expression studies and intracellular Ca2+ measurements. Results: A 42-year-old man presented with slowly progressive muscle weakness and atrophy in all 4 limbs and the trunk. Muscle biopsy and microscopic examination revealed tubular aggregates in his skeletal muscle. Genetic analysis of this family identified a novel heterozygous mutation, c. 1450_1451insGA (p.Ile484ArgfsX21), in stromal interaction molecule 1 (STIM1), a Ca2+ sensor in sarcoplasmic reticulum. We transfected cultured cells with STIM1 and demonstrated that the mutant STIM1 exhibited aggregation-like appearance in shrunk cytoplasm. Furthermore, we revealed that the intracellular Ca2+ influx is decreased by the mutant STIM1. Conclusions: The novel mutation p.Ile484ArgfsX21 is located in the cytoplasmic C-terminal inhibitory domain (CTID) of STIM1. However, all mutations reported so far in TAM reside in the luminal N-terminal EF hand region. The aggregation-like appearance of STIM1 and the decreased intracellular Ca2+ influx in cells transfected with CTID mutant are in sharp contrast to these previous reports. Taken together, these findings indicate that mutations of STIM1 cause TAM through the dysregulation of Ca2+ homeostasis.
A Novel Design Method of AC Side Inductance of Four-Quadrant Converter

Ye Qiang, Cui Hengbin, Du Xinwei, Wu Guangning 1. Sichuan Electric Power Test & Research Institute, No.24, Qinghua Road, Chengdu, P.R. China, 610072 2. School of Electrical Engineering, Southwest Jiaotong University, Chengdu, P.R. China, 610031 3. Electrical Engineering Department, Sichuan Electric Vocational and Technical Collage, Chengdu, P.R. China, 610072 E-mail: sunwind_ye@163.com Abstract—Owing to the merits of low harmonic current component fed to the catenary, unity power factor, bi-directional flowing of energy, excellent dynamic performance, and so forth, four-quadrant converter (4QC) is applied to AC drive locomotive as line-side rectifier. The inductance value of 4QC is vital to the performance of the rectifier. To meet the performance of transient current tracing, the limit of the maximal modulation ratio and catenary voltage fluctuation, a scheme to design AC side inductance of 4QC is presented in the paper. The results of simulation verify the feasibility and correctness of the method.
Analysing the Performance of Optimistic Synchronisation Algorithms in Simulations of Multi-Agent Systems

In this paper we present a detailed analysis of the performance of the Decision Theoretic Read Delay (DTRD) optimistic synchronisation algorithm for simulations of Multi- Agent Systems. We develop an abstract characterisation of the access patterns found in MAS simulations based on the simulation’s degree of coupling and skew. Using this characterisation, we generated stereotypical test cases which we used to compare the performance of the DTRD algorithm with that of Time Warp and time windows. To determine if the test cases reliably predict performance in a real agent simulation, we compared the predictions made by the test cases with performance results from the Boids agent simulation benchmark for a range of simulation parameters. The results indicate that DTRD adapts to the mixtures of coupling cases found in real agent simulations and is capable of tracking changes in coupling during the simulation.
Disruption Risk and Optimal Sourcing in Multitier Supply Networks

We study sourcing in a supply chain with three levels: a manufacturer, tier 1 suppliers, and tier 2 suppliers prone to disruption from, e.g., natural disasters such as earthquakes or floods. The manufacturer may not directly dictate which tier 2 suppliers are used but may influence the sourcing decisions of tier 1 suppliers via contract parameters. The manufacturer’s optimal strategy depends critically on the degree of overlap in the supply chain: if tier 1 suppliers share tier 2 suppliers, resulting in a “diamond-shaped” supply chain, the manufacturer relies less on direct mitigation (procuring excess inventory and multisourcing in tier 1) and more on indirect mitigation (inducing tier 1 suppliers to mitigate disruption risk). We also show that while the manufacturer always prefers less overlap, tier 1 suppliers may prefer a more overlapped supply chain and hence may strategically choose to form a diamond-shaped supply chain. This preference conflict worsens as the manufacturer’s profit margin increases,.
Comparison of Besifloxacin, Gatifloxacin, and Moxifloxacin Against Strains of Pseudomonas aeruginosa With Different Quinolone Susceptibility Patterns in a Rabbit Model of Keratitis

Purpose: Determine the effectiveness of topical besifloxacin, gatifloxacin, and moxifloxacin in treating keratitis caused by 2 strains of Pseudomonas aeruginosa with different quinolone susceptibility profiles. Methods: Minimal inhibitory concentrations (MICs) were determined for each fluoroquinolone. Sequence analysis was performed on the quinolone resistance determining regions of the ciprofloxacin/levofloxacin-resistant strain. Rabbit corneas were injected with 103 colony-forming units (CFU). After 16 hours, phosphate-buffered saline, besifloxacin (6 mg/mL), gatifloxacin (3 mg/mL), or moxifloxacin (5 mg/mL) was applied topically every 15 minutes for 5 doses, then every 30 minutes for 14 doses. Eyes were examined pre- and posttreatment. Corneas were harvested for bacterial quantitation. Results: MICs against the fully susceptible strain were 0.5, 0.25, and 0.5 μg/mL for besifloxacin, gatifloxacin, and moxifloxacin, respectively. The MICs against the ciprofloxacin/levofloxacin-resistant strain were 2, 16, and 32 μg/mL for besifloxacin, gatifloxacin, and moxifloxacin, respectively. Sequence analysis revealed amino acid mutations in all 4 fluoroquinolone target genes. None of the treatments had an effect on clinical severity of eyes infected with the fully susceptible strain (P > 0.05); however, all were effective at significantly reducing the bacterial CFU in the corneas (P < 0.05). For the ciprofloxacin/levofloxacin-resistant strain, clinical scores of besifloxacin-treated eyes were significantly lower than moxifloxacin-treated eyes (P < 0.037). The quantities of ciprofloxacin/levofloxacin-resistant bacteria recovered from corneas of all treatment groups were significantly lower than those recovered from phosphate-buffered saline-treated corneas (P < 0.05). Besifloxacin-treated eyes had significantly lower CFU recovered as compared with that of gatifloxacin- and moxifloxacin-treated eyes (P < 0.05). Conclusions: These results support clinical investigation of the effectiveness of besifloxacin in treating Pseudomonas keratitis.
The transcription cycle of RNA polymerase II in living cells

RNA polymerase II transcribes most eukaryotic genes. Its catalytic subunit was tagged with green fluorescent protein and expressed in Chinese hamster cells bearing a mutation in the same subunit; it complemented the defect and so was functional. Photobleaching revealed two kinetic fractions of polymerase in living nuclei: ∼75% moved rapidly, but ∼25% was transiently immobile (association t1/2 ≈ 20 min) and transcriptionally active, as incubation with 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole eliminated it. No immobile but inactive fraction was detected, providing little support for the existence of a stable holoenzyme, or the slow stepwise assembly of a preinitiation complex on promoters or the nuclear substructure. Actinomycin D decreased the rapidly moving fraction, suggesting that engaged polymerases stall at intercalated molecules while others initiate. When wild-type cells containing only the endogenous enzyme were incubated with [3H]uridine, nascent transcripts became saturated with tritium with similar kinetics (t1/2 ≈ 14 min). These data are consistent with a polymerase being mobile for one half to five sixths of a transcription cycle, and rapid assembly into the preinitiation complex. Then, most expressed transcription units would spend significant times unassociated with engaged polymerases.
Tobacco smoking is causally associated with antipsychotic medication use and schizophrenia, but not with antidepressant medication use or depression.

BACKGROUND Tobacco smoking is more common among patients with schizophrenia and depression than among healthy individuals. We tested the hypothesis that high tobacco smoking intensity is causally associated with antipsychotic medication use, schizophrenia, antidepressant medication use and/or depression in the general population, and compared results with those for chronic obstructive pulmonary disease. METHODS We used self-reported smoking intensity in cigarettes/day and a polymorphism in the CHRNA3 gene cluster (rs1051730) associated with smoking intensity, on 63,296 20-100-year-old individuals from the Danish general population; 23,282 were never-smokers and 40,014 ever-smokers. For schizophrenia, we compared our results with those in the Psychiatric Genomics Consortium. RESULTS In smokers, heterozygotes (CT) and homozygotes (TT) for rs1051730 genotype had higher smoking intensity compared with non-carriers (CC). Furthermore, in ever-smokers homozygotes had increased risk of antipsychotic medication with an odds ratio (OR) of 1.16 [95% confidence interval (CI) 1.02-1.31] compared with non-carriers, whereas in never-smokers the corresponding OR was 1.07 (0.87-1.31) (P-interaction: 0.60). Correspondingly, ORs were 1.60 (0.74-3.47) and 1.02 (0.11-9.10) for schizophrenia (P-interaction: 0.85), 1.02 (0.93-1.13) and 0.99 (0.85-1.15) for antidepressant medication (P-interaction: 0.87), 0.85 (0.66-1.10) and 1.26 (0.87-1.83) for depression (P-interaction: 0.30) and 1.31 (1.16-1.47) and 0.89 (0.58-1.36) for chronic obstructive pulmonary disease (P-interaction: 0.16). Odds ratios per rs1051730 allele for schizophrenia and antipsychotic medication use in ever-smokers in the general population were 1.22 (95% CI: 0.84-1.79) and 1.06 (1.00-1.12). In the Psychiatric Genomics Consortium, the corresponding OR for schizophrenia was 1.06 (1.04-1.08) in ever- and never-smokers combined. CONCLUSION Our data suggest that tobacco smoking could influence the development of psychotic conditions causally, whereas an influence on depression seems unlikely.
Burden of asthma in inner-city elementary schoolchildren: do school-based health centers make a difference?

BACKGROUND About 1400 school-based health centers (SBHCs) provide care to 1.1 million children. However, it is unknown if access to on-site services is associated with a better outcome. OBJECTIVE To compare outcomes including hospitalizations, emergency department visits, and school absenteeism in elementary schoolchildren with asthma who were grouped according to their enrollment at schools that have or do not have SBHCs. SETTING Six elementary schools in The Bronx, NY (4 schools with and 2 without SBHCs). PARTICIPANTS Nine hundred forty-nine inner-city schoolchildren with asthma. DESIGN To collect baseline data for a longitudinal study, we surveyed parents to identify children with asthma, and to obtain information about symptoms and the use of health services in the last 12 months. Participating schools provided absenteeism information. RESULTS Of 6433 parents surveyed, 74% completed the questionnaires. The prevalence of asthma was 19.9% and the morbidity was high-during the previous year, 46.2% had been treated for asthma in an emergency department; 12.6% had been hospitalized. Emergency department use was not associated with SBHCs. However, in univariate and multivariate analyses, the rate of hospitalization was higher among children enrolled at schools without an SBHC (rate ratio, 1.5; 95% confidence interval, 1.1-1.9). In addition, schoolchildren with asthma enrolled in the schools without an SBHC missed more days of school than those enrolled in schools with an SBHC (mean [SD], 21.3 [15.4] vs 18.2 [13.0], respectively; P =.02). CONCLUSION Access to SBHCs was associated with a reduction in the rate of hospitalization and a gain of 3 days of school for schoolchildren who have asthma.
Danger Beyond Dyads: Third-Party Participants in Militarized Interstate Disputes

Stuart Bremer often reminded us that third parties—directly or indirectly—affect the initiation, evolution, and termination of conflict. He encouraged scholars to research the phenomenon of joining behavior further and personally investigated it. Questions about joining behavior are indeed deeply intertwined with a variety of theories of conflict. However, existing records on third-party interventions are limited to states' military involvement in conflict. The limitations imposed by the data can lead researchers to biased or incomplete conclusions about many international phenomena. We heed Bremer's encouragement and present here the results of an effort to collect new evidence on nonneutral (partisan) interventions in militarized interstate disputes for the 1946—2001 period. The data we present differ from existing records in that: (1) they provide information on both third parties' military and nonmilitary activities; (2) they broaden the notion of what constitutes a third party by including coalitions of states, intergovernmental organizations (IGOs), and nongovernmental organizations (NGOs); (3) they expand the investigation framework by recording interventions that occur before and after a militarized dispute. We test the usefulness of the data by exploring the issue of major powers' interventions in conflicts, as Yamamoto and Bremer did in their 1980 “Wider Wars and Restless Nights” article. We offer strong support for Yamamoto and Bremer's finding that major powers drag one another into ongoing conflicts and show how the data may help us raise and answer new and more complex hypotheses about third parties and the dynamics of joining behavior.
An omega-Power of a Finitary Language Which is a Borel Set of Infinite Rank

Omega-powers of finitary languages are omega languages in the form V^omega, where V is a finitary language over a finite alphabet X. Since the set of infinite words over X can be equipped with the usual Cantor topology, the question of the topological complexity of omega-powers naturally arises and has been raised by Niwinski, by Simonnet, and by Staiger. It has been recently proved that for each integer n > 0, there exist some omega-powers of context free languages which are Pi^0_n-complete Borel sets, and that there exists a context free language L such that L^omega is analytic but not Borel. But the question was still open whether there exists a finitary language V such that V^omega is a Borel set of infinite rank. We answer this question in this paper, giving an example of a finitary language whose omega-power is Borel of infinite rank.
An evaluation of interactive test-driven labs with WebIDE in CS0

WebIDE is a framework that enables instructors to develop and deliver online lab content with interactive feedback. The ability to create lock-step labs enables the instructor to guide students through learning experiences, demonstrating mastery as they proceed. Feedback is provided through automated evaluators that vary from simple regular expression evaluation to syntactic parsers to applications that compile and run programs and unit tests. This paper describes WebIDE and its use in a CS0 course that taught introductory Java and Android programming using a test-driven learning approach. We report results from a controlled experiment that compared the use of dynamic WebIDE labs with more traditional static programming labs. Despite weaker performance on pre-study assessments, students who used WebIDE performed two to twelve percent better on all assessments than the students who used traditional labs. In addition, WebIDE students were consistently more positive about their experience in CS0.