Miracle-dz/UniFAR-FTUs huggingface dataset:
A Naturally Occurring Single Nucleotide Polymorphism in the Salmonella SPI-2 Type III Effector srfH/sseI Controls Early Extraintestinal Dissemination

CD18 expressing phagocytes associated with the gastro-intestinal (GI) epithelium can shuttle Salmonella directly into the bloodstream within a few minutes following microbial ingestion. We have previously demonstrated that Salmonella controls the CD18 pathway to deeper tissue, manipulating the migratory properties of infected cells as an unappreciated component of its pathogenesis. We have observed that one type III effector, SrfH (also called SseI) that Salmonella secretes into infected phagocytes manipulates the host protein TRIP6 to stimulate their migration. Paradoxically, SrfH was shown in another study to subvert a different host protein, IQGAP1, in a manner that inhibits the productive motility of such cells, perhaps to avoid interactions with T cells. Here, we resolve the discrepancy. We report that one naturally occurring allele of srfH promotes the migration of infected phagocytes into the bloodstream, while another naturally occurring allele that differs by only a single nucleotide polymorphism (SNP) does not. This SNP determines if the protein contains an aspartic acid or a glycine residue at position 103 and may determine if SrfH binds TRIP6. SrfH Gly103 is a rare allele, but is present in the highly invasive strain Salmonella enterica serovar Typhimurium UK-1 (stands for universal killer). It is also present in the genome of the only sequenced strain belonging to the emerging pandemic Salmonella enterica serovar 4, [5],12,i:-, which is frequently associated with septicemia. Finally, we present evidence that suggests that Gifsy-2, the bacteriophage upon which srfH resides, is present in a clinical isolate of the human-specific pathogen, Salmonella enterica serovar Typhi. These observations may have interesting implications for our understanding of Salmonella pathogenesis.
“Who is this” quiz dialogue system and users' evaluation

In order to design a dialogue system that users enjoy and want to be near for a long time, it is important to know the effect of the system's action on users. This paper describes ldquoWho is thisrdquo quiz dialogue system and its users' evaluation. Its quiz-style information presentation has been found effective for educational tasks. In our ongoing effort to make it closer to a conversational partner, we implemented the system as a stuffed-toy (or CG equivalent). Quizzes are automatically generated from Wikipedia articles, rather than from hand-crafted sets of biographical facts. Network mining is utilized to prepare adaptive system responses. Experiments showed the effectiveness of person network and the relationship of user attribute and interest level.
Adaptive Multicast Routing Based on VP

Two major routing policies were proposed to develop adaptive multicast routing (MST routing in 8]) algorithms in B-ISDN networks. One was to choose the route with the least number of links, and the other was to employ maximum free circuit routing. These methods suuer two major drawbacks: excessive amount of virtual path (VP) status messages to be transferred and performance degradation in asymmetric networks even ignoring the overhead and delay of transferring VP status information. This paper proposes a set of adaptive multicast routing algorithms based on the concept of VP trees. A VP tree which uses VPs dedicated to one-to-one communication as links in a multicast tree is an eecient approach to dimensioning a network allowing multicast communication. The proposed routing algorithms rst attempt to reserve resources of VPs in a default route predetermined with respect to the source and the set of destinations. Our simulation experiments show that the number of VP status messages transferred can be signiicantly reduced. For networks with equivalent bandwidth, the MST routing is able to generate more revenue if the VP status message transferring overhead is ignored. However, for asymmetric networks, which are more practical, the proposed adaptive routing algorithms can produce more revenue.
Informed consent, cultural sensitivity, and respect for persons.

The doctrine of informed consent to medical treatment or research is grounded in the ethical principle of respect for persons that regards individuals as autonomous agents "capable of deliberation about personal goals and of acting under the direction of such deliberation. "1International ethical codes and human rights law appear to accept informed consent as a universal expression of respect for persons. The Nuremberg Code, Helsinki IV, and the Council of International Organizations of Medical Sciences' ethical guidelines focus on the need for full disclosure to enable individuals to make free and informed decisions. Informed consent is also thought to be incorporated into the right to security of the person recognized in the International Bill of Human Rights. The right to autonomy or self-determination, then, is broadly perceived to be a morally necessary method of demonstrating genuine respect for human integrity. See also pp 820 and 826. But is the
Teaching Web Security Using Portable Virtual Labs

We have developed a tool called Secure WEb dEvelopment Teaching (SWEET) to introduce security concepts and practices for web application development. This tool provides introductory tutorials, teaching modules utilizing virtualized hands-on exercises, and project ideas in web application security. In addition, the tool provides pre-configured virtual computer for laboratory exercises. This paper described the design of the tool and the resources it offers to instructors. We also discussed a hands-on course module on cryptography.
Reversed drift in heart rate but increased oxygen uptake at fixed work rate during 24 h ultra‐endurance exercise

In this paper we report a reversed drift in heart rate (HR) but increased oxygen uptake (VO2) during ultra‐endurance exercise. Nine well‐trained male athletes performed 24‐h exercise in a controlled laboratory setting, with alternating blocks of kayaking, running and cycling. Each block included 110 min of exercise and 10 min of rest, with an average work intensity of approximately 55% of respective VO2peak. Blood samples were taken and HR and VO2 measured every 6th hour during steady‐state cycling at fixed work rate. As assumed HR was increased at 6 h by 15 ± 6 beats/min compared with initial level (0 h). Thereafter the drift did not progress continuously, but instead unexpectedly returned toward initial values, although the plasma levels of catecholamines increased continuously during exercise. VO2 was increased by 0.22 ± 0.15 L/min (10%) at 6 h and 0.37 ± 0.18 L/min (17%) at 12 h compared with 0 h, and thereafter remained stable. This implies an increased oxygen pulse (VO2/HR) by approximately 10% at the last half of the 24‐h exercise compared with 0 h. Consequently, sole use of HR would give inaccurate estimates of exercise intensity and energy expenditure during endurance exercise lasting more than 6 h, and different patterns of cardiovascular drift need to be taken into account.
Plasma Fibrin D-Dimer Levels and Risk of Stable Coronary Artery Disease: Results of a Large Case-Control Study

Increased levels of fibrin D-dimer are indicative of a hypercoagulable state, as found in acute coronary syndromes. Few well-controlled studies have assessed D-dimers in patients with stable coronary artery disease (CAD). We measured levels of D-dimers (in ng/mL by enzyme-linked immunosorbent assay) in 312 patients with angiographically proved CAD and stable angina pectoris and in 477 age- and sex-matched healthy blood donors. Demographic characteristics were assessed by a standardized questionnaire, and a complete lipid profile was performed for all subjects. In addition, a variety of other markers of hemostasis and inflammation were measured. The distribution of D-dimer levels was skewed to the right, and plasma median levels were higher in cases than in controls (median: 11.2 vs 2.8 ng/mL;P <0.001). In controls, correlations of D-dimer were found with fibrinogen, plasma viscosity, and interleukin-6. In logistic regression analysis, the age- and sex-adjusted odds ratio (OR) for the presence of CAD was 2.6 (95% confidence interval [CI], 1.9 to 3.5) when the highest quartile of the D-dimer distribution was compared with the combined lower 3 quartiles. The OR did not change appreciably after controlling for nonlipid risk factors (OR, 2.7; 95% CI, 1.9 to 3.9) and remained significant after further adjustment for other hemostatic parameters (OR, 2.4; 95% CI, 1.7 to 3.3) and markers of inflammation (OR, 2.1; 95% CI, 1.5 to 2.9). Plasma D-dimer levels are strongly and independently associated with the presence of CAD in patients with stable angina pectoris. These results support the concept of a contribution of intravascular fibrin to atherothrombogenesis.
Assessing the Extent of Adherence to the Recommended Antenatal Care Content in Malaysia: Room for Improvement

Background Recent papers on monitoring of health services affirmed that while antenatal care (ANC) is an effective measure, quality is still a problem. Quality in maternal services “…involves providing a minimum level of care to all pregnant women…” Yet adherence to a minimum level of recommended ANC content appears to be unmet. Comprehensive review of ANC content rendered in environments with rapid changes in demographic, socio-economic, lifestyle and morbidity was sparse. Malaysia is such a country that has undergone these transitions with tremendous progress in health. However, recent progress in pregnancy outcomes is stagnating. This study aims to analyse adherence to recommended ANC; specifically, to examine the extent of adherence to recommended ANC content and to determine the factors influencing ANC content score. Methods A retrospective cohort study of 522 randomly selected women who used ANC was conducted. Data were extracted from individual records. The study examined adherence to essential ANC guidelines using weighted scoring for physical examination, health screening, case management, and health education. GLM Univariate analysis procedure was used to determine the factors associated with ANC content score. Binary logistic regression was used to assess ANC content level and pregnancy outcomes, controlled for ANC utilisation. Results Around half of the women had <80% of recommended ANC content documented. Health education had the lowest mean score, at around 35%. The low-risk pregnancies had a higher ANC content score than the high-risk pregnancies (78% vs. 75%; P = 0.002). The smallest clinics had a higher ANC content score than the bigger clinics (78% vs. 74–76%; P<0.001). ANC content score among the women with “adequate” ANC utilisation, as defined by the modified Adequacy of Prenatal Care Utilisation Index, was lower than the women with “adequate-plus” ANC utilisation (75% vs. 78%, P<0.001). Assessment of symphysis-fundal height, foetal presentation and foetal heart auscultation were initiated earlier than recommended. Inadequate ANC content was associated with higher prevalence of preterm birth. Conclusions Our findings indicate the presence of issues related to delivery of recommended ANC content. We advocate for all pregnant women to be ensured of adherence to the recommended ANC content. We also recommend monitoring the delivery of health advice. Conforming to recommended timing of initiation for ANC practices is essential due to resource implication and possible implication on maternal wellbeing. The association of inadequate ANC content and preterm birth may be due to lesser opportunities to receive some of the care because of lower number of ANC visits among preterm birth; this may also indicate the importance of having adequate ANC content.
The effects of long-term resistance exercise on the relationship between neurocognitive performance and GH, IGF-1, and homocysteine levels in the elderly

This study aimed to investigate the effects of a long-term resistance exercise intervention on executive functions in healthy elderly males, and to further understand the potential neurophysiological mechanisms mediating the changes. The study assessed forty-eight healthy elderly males randomly assigned to exercise (n = 24) or control (n = 24) groups. The assessment included neuropsychological and neuroelectric measures during a variant of the oddball task paradigm, as well as growth hormone (GH), insulin-like growth factor-1 (IGF-1), and homocysteine levels at baseline and after either a 12 month intervention of resistance exercise training or control period. The results showed that the control group had a significantly lower accuracy rate and smaller P3a and P3b amplitudes in the oddball condition after 12 months. The exercise group exhibited improved reaction times (RTs), sustained P3a and P3b amplitudes, increased levels of serum IGF-1, and decreased levels of serum homocysteine. The changes in IGF-1 levels were significantly correlated with the changes in RT and P3b amplitude of the oddball condition in the exercise group. In conclusion, significantly enhanced serum IGF-1 levels after 12 months of resistance exercise were inversely correlated with neurocognitive decline in the elderly. These findings suggest that regular resistance exercise might be a promising strategy to attenuate the trajectory of cognitive aging in healthy elderly individuals, possibly mediated by IGF-1.
Aalborg Universitet Ten weeks of physical-cognitive-mindfulness training reduces fear-avoidance beliefs about work-related activity

People with chronic musculoskeletal pain often experience pain-related fear of movement and avoidance behavior. The FearAvoidance model proposes a possible mechanism at least partly explaining the development and maintenance of chronic pain. People who interpret pain during movement as being potentially harmful to the organism may initiate a vicious behavioral cycle by generating pain-related fear of movement accompanied by avoidance behavior and hyper-vigilance. This study investigates whether an individually adapted multifactorial approach comprised of biopsychosocial elements, with a focus on physical exercise, mindfulness, and education on pain and behavior, can decrease work-related fear-avoidance beliefs. As part of a large scale 10-week worksite randomized controlled intervention trial focusing on company initiatives to combat workrelated musculoskeletal pain and stress, we evaluated fear-avoidance behavior in 112 female laboratory technicians with chronic neck, shoulder, upper back, lower back, elbow, and hand/wrist pain using the Fear-Avoidance Beliefs Questionnaire at baseline, before group allocation, and again at the post intervention follow-up 10 weeks later. A significant group by time interaction was observed (P<0.05) for work-related fear-avoidance beliefs. The between-group difference at follow-up was –2.2 (–4.0 to –0.5), corresponding to a small to medium effect size (Cohen’s d=0.30). Our study shows that work-related, but not leisure time activity-related, fear-avoidance beliefs, as assessed by the Fear-avoidance Beliefs Questionnaire, can be significantly reduced by 10 weeks of physical-cognitive-mindfulness training in female laboratory technicians with chronic pain. Abbreviations: FAB = Fear-avoidance beliefs, FABQ = Fear-avoidance beliefs questionnaire, PCMT = hysical-cognitivemindfulness training, PRF = Pain-related fear, REF = Reference.
IL‐22 modulates IL‐17A production and controls inflammation and tissue damage in experimental dengue infection

Dengue virus (DENV), a mosquito‐borne flavivirus, is a public health problem in many tropical countries. IL‐22 and IL‐17A are key cytokines in several infectious and inflammatory diseases. We have assessed the contribution of IL‐22 and IL‐17A in the pathogenesis of experimental dengue infection using a mouse‐adapted DENV serotype 2 strain (P23085) that causes a disease that resembles severe dengue in humans. We show that IL‐22 and IL‐17A are produced upon DENV‐2 infection in immune‐competent mice. Infected IL‐22−/− mice had increased lethality, neutrophil accumulation and pro‐inflammatory cytokines in tissues, notably IL‐17A. Viral load was increased in spleen and liver of infected IL‐22−/− mice. There was also more severe liver injury, as seen by increased transaminases levels and tissue histopathology. γδ T cells and NK cells are sources of IL‐17A and IL‐22, respectively, in liver and spleen. We also show that DENV‐infected HepG2 cells treated with rhIL‐22 had reduced cell death and decreased IL‐6 production. IL‐17RA−/− mice were protected upon infection and IL‐17A‐neutralizing‐Ab‐treatment partially reversed the phenotype observed in IL‐22−/−‐infected mice. We suggest that disrupting the balance between IL‐22 and IL‐17A levels may represent an important strategy to reduce inflammation and tissue injury associated with severe dengue infection.
Control and Stabilization of Runaway Electron Emission at the Delay Stage of Pulsed Breakdown in an Overvolted Atmospheric Gap

In this paper, we present investigations of picosecond effects in atmospheric gaps at the stage of a pulsed breakdown delay. It is shown that in centimeter gaps with rapid achievement of multiple overvoltages, the breakdown point, its stability, and development time are determined by the advance emission of runaway electrons. Methods for controlling and stabilizing the temporary and energy characteristics of the flows of these particles are proposed.
The Young Men's Clinic: addressing men's reproductive health and responsibilities.

Interest in men’s health including their sexual and reproductive health has been growing over the past two decades. The 1994 International Conference on Population and Development in Cairo and the 1995 Fourth World Conference on Women in Beijing both recognized the effect of men’s behavior on women’s health highlighted the importance of shared responsibility and sparked interest in developing interventions to increase male involvement in reproductive health programs. A 2002 report by The Alan Guttmacher Institute emphasized that the sexual and reproductive health concerns of men are important in their own right not only because males play important roles as fathers and sexual partners. The National Survey of Adolescent Males the Youth Risk Behavior Survey and studies and reports sponsored or produced by other organizations have significantly contributed to the growing body of knowledge about men’s sexual and reproductive health concerns beliefs attitudes and behaviors. Since 1997 the Office of Family Planning in the Office of Population Affairs at the Department of Health and Human Services has funded diverse community-based programs to learn how to engage with and provide reproductive health services to males. This special report describes sexual and reproductive health services and how they have evolved at one of those programs—the Young Men’s Clinic an ambulatory clinic for adolescent and young adult males in New York City. (excerpt)
Respiratory Variations in Pulse Pressure Reflect Central Hypovolemia during Noninvasive Positive Pressure Ventilation

Background. Correct volume management is essential in patients with respiratory failure. We investigated the ability of respiratory variations in noninvasive pulse pressure (ΔPP), photoplethysmographic waveform amplitude (ΔPOP), and pleth variability index (PVI) to reflect hypovolemia during noninvasive positive pressure ventilation by inducing hypovolemia with progressive lower body negative pressure (LBNP). Methods. Fourteen volunteers underwent LBNP of 0, −20, −40, −60, and −80 mmHg for 4.5 min at each level or until presyncope. The procedure was repeated with noninvasive positive pressure ventilation. We measured stroke volume (suprasternal Doppler), ΔPP (Finapres), ΔPOP, and PVI and assessed their association with LBNP-level using linear mixed model regression analyses. Results. Stroke volume decreased with each pressure level (−11.2 mL, 95% CI −11.8, −9.6, P < 0.001), with an additional effect of noninvasive positive pressure ventilation (−3.0 mL, 95% CI −8.5, −1.3, P = 0.009). ΔPP increased for each LBNP-level (1.2%, 95% CI 0.5, 1.8, P < 0.001) and almost doubled during noninvasive positive pressure ventilation (additional increase 1.0%, 95% CI 0.1, 1.9, P = 0.003). Neither ΔPOP nor PVI was significantly associated with LBNP-level. Conclusions. During noninvasive positive pressure ventilation, preload changes were reflected by ΔPP but not by ΔPOP or PVI. This implies that ΔPP may be used to assess volume status during noninvasive positive pressure ventilation.
THE MEASUREMENT OF WORK: HIERACHICAL REPRESENTATION OF THE MULTIMETHOD JOB DESIGN QUESTIONNAIRE

To study the changing nature of work, researchers need measures of work that are valid and comprehensive. One potentially useful measure of work is the Multimethod Job Design Questionnaire (MJDQ; Campion, 1988), which was developed to assess 4 general approaches to work design (i.e., motivational, mechanistic, biological, perceptualmotor). Although the MJDQ holds promise as a general measure of work, little information is available regarding its psychometric properties. This study examines the MJDQ, using alternative hierarchical factor structures to represent work at varying levels of abstraction. Little support was found for the 4-factor structure corresponding to the work design approaches underling the MJDQ or for various hierarchical factor structures that simultaneously depicted general and specific aspects of work. However, a 10-factor first-order model received good support and may provide a useful basis for scoring and interpreting the MJDQ in future research.
In Vitro and In Vivo Evaluation of Essential Oil from Artemisia absinthium L. Formulated in Nanocochleates against Cutaneous Leishmaniasis

Background: Leishmaniasis is a zoonotic disease caused by protozoan parasites from Leishmania genus. Currently, there are no effective vaccines available and the available therapies are far from ideal. In particular, the development of new therapeutic strategies to reduce the infection caused by Leishmania amazonensis could be considered desirable. Different plant-derived products have demonstrated antileishmanial activity, including the essential oil (EO) from Artemisia absinthium L. (EO-Aa), Asteraceae. Methods: In the present study, the EO-Aa formulated in nanocochleates (EO-Aa-NC) was investigated in vitro against intracellular amastigotes of L. amazonensis and non-infected macrophages from BALB/c mice. In addition, the EO-Aa-NC was also evaluated in vivo against on experimental cutaneous leishmaniasis, which body weight, lesion progression, and parasite load were determined. Results: EO-Aa-NC displayed IC50 values of 21.5 ± 2.5 μg/mL and 27.7 ± 5.6 μg/mL against intracellular amastigotes of L. amazonensis and non-infected peritoneal macrophage, respectively. In the animal model, the EO-Aa-NC (30 mg/kg/intralesional route/every 4 days 4 times) showed no deaths or weight loss greater than 10%. In parallel, the EO-Aa-NC suppressed the infection in the murine model by approximately 50%, which was statistically superior (p < 0.05) than controls and mice treated with EO-Aa. In comparison with Glucantime®, EO-Aa-NC inhibited the progression of infection as efficiently (p > 0.05) as administration of the reference drug. Conclusions: Encochleation of EO-Aa resulted in a stable, tolerable, and efficacious antileishmanial formulation, facilitating systemic delivery of EO, with increased activity compared to administration of the free EO-Aa. This new formulation shows promising potential to future studies aimed at a new therapeutic strategy to treat leishmaniasis.