Miracle-dz/UniFAR-FTUs huggingface dataset:
Intermittent hypercapnia‐induced phrenic long‐term depression is revealed after serotonin receptor blockade with methysergide in anaesthetized rats

What is the central question of this study? Intermittent hypercapnia is a concomitant feature of breathing disorders. Hypercapnic stimuli evoke a form of respiratory plasticity known as phrenic long‐term depression in experimental animals. This study was performed to investigate the putative role of serotonin receptors in the initiation of phrenic long‐term depression in anaesthetized rats. What is the main finding and its importance? Phrenic nerve long‐term depression was revealed in animals pretreated with the serotonin broad‐spectrum antagonist, methysergide. This study highlights that serotonin receptors modulate respiratory plasticity evoked by acute intermittent hypercapnia in anaesthetized rats.
Limits on multiple instruction issue

This paper investigates the limitations on designing a processor which can sustain an execution rate of greater than one instruction per cycle on highly-optimized, non-scientific applications. We have used trace-driven simulations to determine that these applications contain enough instruction independence to sustain an instruction rate of about two instructions per cycle. In a straightforward implementation, cost considerations argue strongly against decoding more than two instructions in one cycle. Given this constraint, the efficiency in instruction fetching rather than the complexity of the execution hardware limits the concurrency attainable at the instruction level.
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases

Gene knockout is the most powerful tool for determining gene function or permanently modifying the phenotypic characteristics of a cell. Existing methods for gene disruption are limited by their efficiency, time to completion, and/or the potential for confounding off-target effects. Here, we demonstrate a rapid single-step approach to targeted gene knockout in mammalian cells, using engineered zinc-finger nucleases (ZFNs). ZFNs can be designed to target a chosen locus with high specificity. Upon transient expression of these nucleases the target gene is first cleaved by the ZFNs and then repaired by a natural—but imperfect—DNA repair process, nonhomologous end joining. This often results in the generation of mutant (null) alleles. As proof of concept for this approach we designed ZFNs to target the dihydrofolate reductase (DHFR) gene in a Chinese hamster ovary (CHO) cell line. We observed biallelic gene disruption at frequencies >1%, thus obviating the need for selection markers. Three new genetically distinct DHFR−/− cell lines were generated. Each new line exhibited growth and functional properties consistent with the specific knockout of the DHFR gene. Importantly, target gene disruption is complete within 2–3 days of transient ZFN delivery, thus enabling the isolation of the resultant DHFR−/− cell lines within 1 month. These data demonstrate further the utility of ZFNs for rapid mammalian cell line engineering and establish a new method for gene knockout with application to reverse genetics, functional genomics, drug discovery, and therapeutic recombinant protein production.
Expressed Emotions in Patients with Obsessive Compulsive Disorder: A Case Control Study

Background: Expressed emotion (EE) is the attitude that the relatives show towards the illness and the person. EE is identified as a direct factor in the relapse of patients with psychological disorders. Literature on EE in anxiety disorders is limited. Role of EE in obsessive compulsive disorder (OCD) may help in better understanding of the role of social factors in OCD. Aim: To compare EE in patients with OCD and compare with controls. Materials and Methods: The sample included 30 cases and 30 age and sex matched controls. The patients were diagnosed as having OCD using International Classification of Diseases-10 Diagnostic Criteria for Research (ICD-10 DCR) criteria. Yale-Brown Obsessive Compulsive Scale (Y-BOCS) was used to assess severity of OCD among patients. General health questionnaire (GHQ) was used to rule out any psychiatric disorder among control population. EE was assessed in cases and controls using Family Emotional Involvement and Criticism Scale (FEICS). Chi-square test and t-test were used to assess the difference between two groups. Results: OCD patients in comparison controls had significantly increased total FEICS score (P = 0.001). There was an increase in both subscales of perceived criticism (PC) with P = 0.001 and emotional involvement (EI) with P = 0.001 in patients with OCD than controls. Conclusion: EE are significantly increased in patients with OCD. EE should be assessed regularly in patients with OCD.
Membrane-cytoskeleton dynamics in rat parietal cells: mobilization of actin and spectrin upon stimulation of gastric acid secretion

The gastric parietal (oxyntic) cell is presented as a model for studying the dynamic assembly of the skeletal infrastructure of cell membranes. A monoclonal antibody directed to a 95-kD antigen of acid- secreting membranes of rat parietal cells was characterized as a tracer of the membrane movement occurring under physiological stimuli. The membrane rearrangement was followed by immunocytochemistry both at the light and electron microscopic level on semithin and thin frozen sections from resting and stimulated rat gastric mucosa. Double labeling experiments demonstrated that a specific and massive mobilization of actin, and to a lesser extent of spectrin (fodrin), was involved in this process. In the resting state, actin and spectrin were mostly localized beneath the membranes of all cells of the gastric gland, whereas the bulk of acid-secreting membranes appeared diffusely distributed in the cytoplasmic space of parietal cells without any apparent connection with cytoskeletal proteins. In stimulated cells, both acid-secreting material and actin (or spectrin) extensively colocalized at the secretory apical surface of parietal cells, reflecting that acid-secreting membranes were now exposed at the lumen of the secretory canaliculus and that this insertion was stabilized by cortical proteins. The data are compatible with a model depicting the membrane movement occurring in parietal cells as an apically oriented insertion of activated secretory membranes from an intracellular storage pool. The observed redistribution of actin and spectrin argues for a direct control by gastric acid secretagogues of the dynamic equilibrium existing between nonassembled (or preassembled) and assembled forms of cytoskeletal proteins.
Human keratinocytes are a source for tumor necrosis factor alpha: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light

Tumor necrosis factor alpha (TNF-alpha), in addition to being cytotoxic for certain tumor cells, has turned out as a multifunctional cytokine that is involved in the regulation of immunity and inflammation. Since human keratinocytes have been demonstrated to be a potent source of various cytokines, it was investigated whether epidermal cells synthesize and release TNF-alpha. Supernatants derived from normal human keratinocytes (HNK) and human epidermoid carcinoma cell lines (KB, A431) were tested both in a TNF-alpha-specific ELISA and a bioassay. In supernatants of untreated epidermal cells, no or minimal TNF-alpha activity was found, while after stimulation with lipopolysaccharide (LPS) or ultraviolet (UV) light, significant amounts were detected. Western blot analysis using an antibody directed against human TNF-alpha revealed a molecular mass of 17 kD for keratinocyte- derived TNF-alpha. These biological and biochemical data were also confirmed by Northern blot analysis revealing mRNA specific for TNF- alpha in LPS- or ultraviolet B (UVB)-treated HNK and KB cells. In addition, increased TNF-alpha levels were detected in the serum obtained from human volunteers 12 and 24 h after a single total body UVB exposure, which caused a severe sunburn reaction. These findings indicate that keratinocytes upon stimulation are able to synthesize and release TNF-alpha, which may gain access to the circulation. Thus, TNF- alpha in concert with other epidermal cell-derived cytokines may mediate local and systemic inflammatory reactions during host defense against injurious events caused by microbial agents or UV irradiation.
Award-winning and Finalist Abstracts from the 69th Scientific Sessions of the American Heart Association New Orleans, LA, USA November 10–13, 1996

s presented, all of the AsidPacific authors-numbering over 300-could not be presented in this issue. The following pages include only the award-winning and award-finalist Asian abstracts from the sessions.s from the sessions. Approximately 34,000 people attended the meeting, one-fourth of whom came from outside the United States. Approximately 1600 registrants from the AdPacific region attended the meeting, representing about 19% of the total foreign attendees. Nearly 7% of the approximately4,360presentations were written in all or in part by AsidPacific authors. Acknowledgment for all abstracts on pages 27A-36A: Reproduced with permission. Circrrltltiori 0 American Heart Association 1996 1996, VOL. 4, NO. 4 Presented at the 69th Scientific Sessions of the American Heart Association in New Orleans, LA, USA, held November 10-13, ASIAN CARDIOVASCULAR & THORACIC ANNALS 27A
Automatic memory reclamation for lock-free data structures

Lock-free data-structures are widely employed in practice, yet designing lock-free memory reclamation for them is notoriously difficult. In particular, all known lock-free reclamation schemes are ``manual'' in the sense that the developer has to specify when nodes have retired and may be reclaimed. Retiring nodes adequately is non-trivial and often requires the modification of the original lock-free algorithm. In this paper we present an automatic lock-free reclamation scheme for lock-free data-structures in the spirit of a mark-sweep garbage collection. The proposed algorithm works with any normalized lock-free algorithm and with no need for the programmer to retire nodes or make changes to the algorithm. Evaluation of the proposed scheme on a linked-list and a hash table shows that it performs similarly to the best manual (lock-free) memory reclamation scheme.
Anatomical Substrates of Visual and Auditory Miniature Second-language Learning

Longitudinal changes in brain activity during second language (L2) acquisition of a miniature finite-state grammar, named Wernickese, were identified with functional magnetic resonance imaging (fMRI). Participants learned either a visual sign language form or an auditory-verbal form to equivalent proficiency levels. Brain activity during sentence comprehension while hearing/viewing stimuli was assessed at low, medium, and high levels of proficiency in three separate fMRI sessions. Activation in the left inferior frontal gyrus (Broca's area) correlated positively with improving L2 proficiency, whereas activity in the right-hemisphere (RH) homologue was negatively correlated for both auditory and visual forms of the language. Activity in sequence learning areas including the premotor cortex and putamen also correlated with L2 proficiency. Modality-specific differences in the blood oxygenation level-dependent signal accompanying L2 acquisition were localized to the planum temporale (PT). Participants learning the auditory form exhibited decreasing reliance on bilateral PT sites across sessions. In the visual form, bilateral PT sites increased in activity between Session 1 and Session 2, then decreased in left PT activity from Session 2 to Session 3. Comparison of L2 laterality (as compared to L1 laterality) in auditory and visual groups failed to demonstrate greater RH lateralization for the visual versus auditory L2. These data establish a common role for Broca's area in language acquisition irrespective of the perceptual form of the language and suggest that L2s are processed similar to first languages even when learned after the critical period. The right frontal cortex was not preferentially recruited by visual language after accounting for phonetic/structural complexity and performance.
Personality traits of hospital pharmacists: toward a better understanding of factors influencing pharmacy practice change.

BACKGROUND The profession of pharmacy has adopted a mandate to become more patient-centred; however, significant change in this direction has not been achieved. OBJECTIVE To characterize the personality traits of hospital pharmacists in one Canadian province, to provide insights into potential barriers to practice change. METHODS A cross-sectional survey of hospital pharmacists was conducted in Alberta, Canada. An invitation to participate was sent to all 766 hospital pharmacists practising in the province's 2 health service organizations. The survey was based on the Big Five Inventory, a validated, reliable instrument that uses a 5-point Likert scale to measure the traits of extraversion, agreeableness, conscientiousness, neuroticism, and openness. RESULTS Of the 347 pharmacists who completed the survey (45% response rate), the majority (297 [86%]) were staff pharmacists working full time in an urban setting. The average age of respondents was 41 years (standard deviation [SD] 11 years), and the average period in practice was 17 years (SD 11 years). Respondents' mean scores were 3.2 (SD 0.7) on extraversion, 3.8 (SD 0.4) on agreeableness, 4.0 (SD 0.4) on conscientiousness, 2.5 (SD 0.7) on neuroticism, and 3.5 (SD 0.6) on openness. Total frequency counts revealed that respondents tended toward stronger expression of extraversion, agreeableness, conscientiousness, and openness and low levels of neuroticism (with the latter indicating stability). CONCLUSION The Big Five Inventory represents a novel approach to examining pharmacists' change-related behaviours. Improving understanding of hospital pharmacists' personality traits will provide insights for the development of training and support programs tailored specifically to this group.
Treatment of Non-Alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) is now the commonest cause of chronic liver disease in developed countries. Treatment depends on the stage of disease, and non-invasive methods for risk stratification are urgently needed. Lifestyle modification (aimed at weight loss and increasing physical activity) and management of the features of metabolic syndrome are vital for all patients with NAFLD. Metformin is the first-line therapy for diabetic patients with NAFLD and also reduces the risk of hepatocellular carcinoma. Clinicians should have a low threshold for introducing a statin for the management of dyslipidaemia. Antihypertensive agents that target the renin-angiotensin system should be first-line in NAFLD for the management of hypertension. For patients with progressive disease, liver-directed pharmacotherapy with vitamin E should be considered. Non-alcoholic steatohepatitis cirrhosis is an increasingly common indication for liver transplantation.
Rate Adaptive Based Resource Allocation with Proportional Fairness Constraints in OFDMA Systems

Orthogonal frequency division multiple access (OFDMA), which is widely used in the wireless sensor networks, allows different users to obtain different subcarriers according to their subchannel gains. Therefore, how to assign subcarriers and power to different users to achieve a high system sum rate is an important research area in OFDMA systems. In this paper, the focus of study is on the rate adaptive (RA) based resource allocation with proportional fairness constraints. Since the resource allocation is a NP-hard and non-convex optimization problem, a new efficient resource allocation algorithm ACO-SPA is proposed, which combines ant colony optimization (ACO) and suboptimal power allocation (SPA). To reduce the computational complexity, the optimization problem of resource allocation in OFDMA systems is separated into two steps. For the first one, the ant colony optimization algorithm is performed to solve the subcarrier allocation. Then, the suboptimal power allocation algorithm is developed with strict proportional fairness, and the algorithm is based on the principle that the sums of power and the reciprocal of channel-to-noise ratio for each user in different subchannels are equal. To support it, plenty of simulation results are presented. In contrast with root-finding and linear methods, the proposed method provides better performance in solving the proportional resource allocation problem in OFDMA systems.
Optimal Partition Trees

We revisit one of the most fundamental classes of data structure problems in computational geometry: range searching. Matoušek (Discrete Comput. Geom. 10:157–182, 1993) gave a partition tree method for d-dimensional simplex range searching achieving O(n) space and O(n1−1/d) query time. Although this method is generally believed to be optimal, it is complicated and requires O(n1+ε) preprocessing time for any fixed ε>0. An earlier method by Matoušek (Discrete Comput. Geom. 8:315–334, 1992) requires O(nlogn) preprocessing time but O(n1−1/dlogO(1)n) query time. We give a new method that achieves simultaneously O(nlogn) preprocessing time, O(n) space, and O(n1−1/d) query time with high probability. Our method has several advantages: It is conceptually simpler than Matoušek’s O(n1−1/d)-time method. Our partition trees satisfy many ideal properties (e.g., constant degree, optimal crossing number at almost all layers, and disjointness of the children’s cells at each node). It leads to more efficient multilevel partition trees, which are needed in many data structuring applications (each level adds at most one logarithmic factor to the space and query bounds, better than in all previous methods). A similar improvement applies to a shallow version of partition trees, yielding O(nlogn) time, O(n) space, and O(n1−1/⌊d/2⌋) query time for halfspace range emptiness in even dimensions d≥4. Numerous consequences follow (e.g., improved results for computing spanning trees with low crossing number, ray shooting among line segments, intersection searching, exact nearest neighbor search, linear programming queries, finding extreme points, …). It is conceptually simpler than Matoušek’s O(n1−1/d)-time method. Our partition trees satisfy many ideal properties (e.g., constant degree, optimal crossing number at almost all layers, and disjointness of the children’s cells at each node). It leads to more efficient multilevel partition trees, which are needed in many data structuring applications (each level adds at most one logarithmic factor to the space and query bounds, better than in all previous methods). A similar improvement applies to a shallow version of partition trees, yielding O(nlogn) time, O(n) space, and O(n1−1/⌊d/2⌋) query time for halfspace range emptiness in even dimensions d≥4.
Toward a New Generation of Cross-Cultural Research

In this article, we describe how cross-cultural research methodologies have evolved, with each phase of research addressing limitations of a previous one. We describe briefly the three previous phases and argue for embarking on a fourth phase that empirically establishes linkages between the active cultural ingredients hypothesized to cause between-country differences and the observed differences themselves. We discuss theoretical considerations and possible empirical methods to establish such linkages, and urge researchers to seriously consider incorporating these kinds of linkage studies in their programs of research.
Online Learning of Approximate Dependency Parsing Algorithms

In this paper we extend the maximum spanning tree (MST) dependency parsing framework of McDonald et al. (2005c) to incorporate higher-order feature representations and allow dependency structures with multiple parents per word. We show that those extensions can make the MST framework computationally intractable, but that the intractability can be circumvented with new approximate parsing algorithms. We conclude with experiments showing that discriminative online learning using those approximate algorithms achieves the best reported parsing accuracy for Czech and Danish.
Cluster Stability for Finite Samples

Over the past few years, the notion of stability in data clustering has received growing attention as a cluster validation criterion in a sample-based framework. However, recent work has shown that as the sample size increases, any clustering model will usually become asymptotically stable. This led to the conclusion that stability is lacking as a theoretical and practical tool. The discrepancy between this conclusion and the success of stability in practice has remained an open question, which we attempt to address. Our theoretical approach is that stability, as used by cluster validation algorithms, is similar in certain respects to measures of generalization in a model-selection framework. In such cases, the model chosen governs the convergence rate of generalization bounds. By arguing that these rates are more important than the sample size, we are led to the prediction that stability-based cluster validation algorithms should not degrade with increasing sample size, despite the asymptotic universal stability. This prediction is substantiated by a theoretical analysis as well as some empirical results. We conclude that stability remains a meaningful cluster validation criterion over finite samples.
Depositional environments and sources for the middle Eocene Fulra Limestone Formation, Kachchh Basin, western India: Evidences from facies analysis, mineralogy, and geochemistry

The middle Eocene Fulra Limestone Formation of the Kachchh Basin, western India was investigated to interpret depositional environments and sources of limestones based on facies analysis, mineralogy, and major, trace, and rare earth elements (REEs) geochemistry. Facies stacking pattern of the Fulra Limestone Formation exhibits an overall shallowing‐upward sedimentary succession developed in the middle to inner ramp to lagoonal set‐up. Staining of the thin sections and X‐ray diffraction patterns show dominance of calcite mineral. The CaO, ranging from 40.45% to 49.69%, is the dominant oxide among the major oxides followed by SiO2 (4.04–10.3%). The dominance of CaO over MgO suggests that the major mineral phase has been calcite and no dolomitization occurred during the deposition of the Fulra limestone. The low proportion of SiO2 and Al2O3 suggests low siliciclastic input during the precipitation of the Fulra limestone that is confirmed by the positive correlation of ∑REE with Al2O3, Fe2O3, Ni, Th, Sc, and Y and the negative correlation of SiO2, Al2O3, and Sr with CaO. The average value of REE contents in the Fulra Limestone Formation are lower (*16.02 ppm) than the average value of typical marine carbonates (*28 ppm). The Post‐Archean Australian Shale‐normalized REE + Y patterns of the Fulra limestone exhibit seawater like shale‐normalized REE + Y pattern, whereas slightly lower average value of Y/Ho ratio (34.60) than the typical seawater value (~44–74) suggests modification of the seawater, to some extent, by input of freshwater in a coastal environment. The REEs of the Fulra Limestone Formation have similarity with the carbonates of the Arabian Sea and/or shallow marine carbonates with exception of a few elements. It is envisaged from this study that the Fulra limestone was deposited in a shallow marine to coastal set‐up where mixing of the continental material in seawater was feasible.