Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluating the efficacy of restoration plantings through DNA barcoding of frugivorous bird diets

Frugivores are critical components of restoration programs because they are seed dispersers. Thus, knowledge about bird–plant trophic relationships is essential in the evaluation of the efficacy of restoration processes. Traditionally, the diet of frugivores is characterized by microscopically identifying plant residues in droppings, which is time‐consuming, requires botanical knowledge, and cannot be used for fragments lacking detectable morphological characteristics (e.g., fragmented seeds and skins). We examined whether DNA barcoding can be used as a universal tool to rapidly characterize the diet of a frugivorous bird, Eurasian blackcap (Sylvia atricapilla). We used the DNA barcoding results to assess restoration efforts and monitor the diversity of potentially dispersed plants in a protected area in northern Italy. We collected 642 Eurasian Blackcap droppings at the restored site during the autumn migration over 3 years. Intact seeds and fragmented plant material were analyzed at 2 plastidial barcode loci (rbcL and trnH‐psbA), and the resulting plant identifications were validated by comparison with a reference molecular data set of local flora. At least 17 plant species, including 7 of the 11 newly transplanted taxa, were found. Our results demonstrate the potential for DNA barcoding to be used to monitor the effectiveness of restoration plantings and to obtain information about fruit consumption and dispersal of invasive or unexpected plant species. Such an approach provides valuable information that could be used to study local plant biodiversity and to survey its evolution over time.
Simplified and standardized intrapartum management can yield high rates of successful VBAC in spontaneous labor

Abstract Objective: To examine the outcomes of vaginal birth after cesarean (VBAC) in women, in spontaneous labor, delivering after 37 weeks’ gestation at an institution where trial of labor after a previous cesarean delivery (TOLAC) is encouraged and management of labor is standardized. Methods: This retrospective cohort study included 3071 women with one previous cesarean only and no vaginal delivery who underwent a trial of labor from 2001 to 2011. Women were managed using the standardized “active management of labor” intrapartum protocol. Outcomes and characteristics of women who delivered vaginally were compared with those who required cesarean delivery. Results: In spontaneous labor in their second pregnancy, those who attempted TOLAC had a 72.5% (1611/2222) rate of successful VBAC. Women who had a successful VBAC had smaller babies (3584 ± 452 g versus 3799 ± 489 g; p < 0.0001) at earlier gestations than those who had a repeat intrapartum cesarean delivery. They also required less intrapartum intervention, such as oxytocin augmentation (14.5% [234/1611] versus 41% [251/611]; p < 0.0001) and epidural anesthesia (64.8% [1044/1611] versus 82.8% [506/611]; p < 0.0001). The rate of uterine rupture was 0.54% (12/2222), while the rate of peri-partum hysterectomy was 0.18% (4/2222). Conclusion: This study shows that serious complications associated with TOLAC are rare providing intrapartum care and decision-making is made simple for the benefit of staff and patients alike. This is achieved through a standardized labor management protocol.
Flocculation of low algae concentration water using polydiallyldimethylammonium chloride coupled with polysilicate aluminum ferrite

ABSTRACT The combined application of polydiallyldimethylammonium chloride (PDADMAC) and polysilicate aluminum ferrite (PSFA) was investigated to treat low algae density water samples, in which Microcystis aeruginosa is one of the dominant species. coagulation performance of M. aeruginosa was studied with regard to algal removal, Algal density was evaluated by determining the change in the optical density of the algal culture suspension at 680 nm and chlorophyll a. The dissolved organic matter, cellular morphology, viability, and recovery of M. aeruginosa cells after flocculation and sedimentation were also included. In addition, the effects of pH and addition order of PSFA and PDADMAC on algal removal were investigated. The removal efficiency of algae coagulated using combined PDADMAC and PSFA was improved by 34.5% and 19.3%, respectively. The organic matter removal was also enhanced. The optimum pH range for algal removal is 7.0–10.0, and the preferable addition sequence is the simultaneous addition of PDADMAC and PSFA. Scanning electron microscopy observation indicated that the combined usage of PDADMAC and PSFA caused no damage to the algal cell. Moreover, the experiment on algal recovery demonstrated that PDADMAC has bacteriostatic ability.
FRP Reinforcement for Concrete Structures

This article discusses commercially available fiber reinforced plastics reinforcement made of carbon (CFRP), aramid (AFRP), or glass fibers (GFRP) impregnated with resin, as well as some aramid filaments without a resin matrix. The advantages of FRP reinforcement are listed, as well as its disadvantages. CFRP has the highest tensile modulus of elasticity of all the FRPs. The article notes poly acrylonitirile CFRP and the unidrectional carbon fiber composite cable made in Tokyo, Japan. Coal tar pitch-based continuous carbon fiber and an epoxy resin are used in CFRP bars made in Japan, and graphite fibers are used in rods made in Britain. AFRP reinforcement is manufactured as ropes and rods. GFRP is the least expensive type of FRP reinforcement and used for prestressing tendons, as well as for nonprestressing applications. Fiber-based grid and grating reinforcements are discussed, including continuous grids from continuous glass, carbon or aramid fibers.
ESR1 and endocrine therapy resistance: more than just mutations.

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Leveraging Health Care Simulation Technology for Human Factors Research

Objective: We describe health care simulation, designed primarily for training, and provide examples of how human factors experts can collaborate with health care professionals and simulationists—experts in the design and implementation of simulation—to use contemporary simulation to improve health care delivery. Background: The need—and the opportunity—to apply human factors expertise in efforts to achieve improved health outcomes has never been greater. Health care is a complex adaptive system, and simulation is an effective and flexible tool that can be used by human factors experts to better understand and improve individual, team, and system performance within health care. Method: Expert opinion is presented, based on a panel delivered during the 2014 Human Factors and Ergonomics Society Health Care Symposium. Results: Diverse simulators, physically or virtually representing humans or human organs, and simulation applications in education, research, and systems analysis that may be of use to human factors experts are presented. Examples of simulation designed to improve individual, team, and system performance are provided, as are applications in computational modeling, research, and lifelong learning. Conclusion: The adoption or adaptation of current and future training and assessment simulation technologies and facilities provides opportunities for human factors research and engineering, with benefits for health care safety, quality, resilience, and efficiency. Application: Human factors experts, health care providers, and simulationists can use contemporary simulation equipment and techniques to study and improve health care delivery.
Lactoferrin promotes murine C2C12 myoblast proliferation and differentiation and myotube hypertrophy

Lactoferrin (Lf) is a multifunctional glycoprotein, which promotes the proliferation of murine C2C12 myoblasts. In the present study, it was investigated how Lf promotes myoblast proliferation and whether Lf promotes myoblast differentiation or induces myotube hypertrophy. Lf promoted the proliferation of myoblasts in a dose-dependent manner. Myoblast proliferation increased on day 3 when myoblasts were cultured in the presence of Lf for three days and also when myoblasts were cultured in the presence of Lf for the first day and in the absence of Lf for the subsequent two days. In addition, Lf induced the phosphorylation of extracellular signal-regulated kinase (ERK)1/2 in myoblasts. The mitogen-activated protein kinase kinase 1/2 inhibitor U0126 inhibited Lf-induced ERK1/2 phosphorylation and repressed Lf-promoted myoblast proliferation. C2C12 myoblasts, myotubes and skeletal muscle expressed low-density lipoprotein receptor-related protein (LRP)1 mRNA and Lf-promoted myoblast proliferation was attenuated by an LRP1 antagonist or LRP1 gene silencing. The knockdown of LRP1 repressed Lf-induced phosphorylation of ERK1/2. Furthermore, when myoblasts were induced to differentiate, Lf increased the expression of the myotube-specific structural protein, myosin heavy chain (MyHC) and promoted myotube formation. Knockdown of LRP1 repressed Lf-induced MyHC expression. Lf also increased myotube size following differentiation. These results indicate that Lf promotes myoblast proliferation and differentiation, at least partially through LRP1 and also stimulates myotube hypertrophy.
Measurement of Pro-Islet Amyloid Polypeptide (1–48) in Diabetes and Islet Transplants

Context Islet amyloid is a feature of β-cell failure in type 2 diabetes (T2D) and type 1 diabetes (T1D) recipients of islet transplants. Islet amyloid contains islet amyloid polypeptide (IAPP; amylin), a circulating peptide that is produced in β cells by processing of its precursor, proIAPP1-67, via an intermediate form, proIAPP1-48. Elevated proinsulin to C-peptide ratios in the plasma of persons with diabetes suggest defects in β-cell prohormone processing. Objective Determine whether plasma levels of precursor forms of IAPP are elevated in diabetes. Design, Setting, and Patients We developed an immunoassay to detect proIAPP1-48 in human plasma, and we determined the ratio of proIAPP1-48 to mature IAPP in subjects with T1D, T2D, recipients of islet transplants, and healthy controls. Results The proIAPP1-48 immunoassay had a limit of detection of 0.18 ± 0.06 pM and cross-reactivity with intact proIAPP1-67 <15%. Healthy individuals had plasma concentrations of proIAPP1-48 immunoreactivity of 1.5 ± 0.2 pM and a proIAPP1-48 to total IAPP ratio of 0.28 ± 0.03. Plasma concentrations of proIAPP1-48 immunoreactivity were not significantly different in subjects with T2D but were markedly increased in T1D recipients of islet transplants. Children and adults with T1D had reduced mature IAPP levels relative to age-matched controls but an elevated ratio of proIAPP1-48 to total IAPP. Conclusion The β cells in T1D and islet transplants have impaired processing of the proIAPP1-48 intermediate. The ratio of proIAPP1-48-to-IAPP immunoreactivity may have value as a biomarker of β-cell stress and dysfunction.
Perceived quality of life and acceptance of illness in people with type 2 diabetes mellitus

Objectives Type 2 diabetes exerts a significant impact on the patient’s quality of life. Its chronic nature, incurability and complications weaken the motivation of patients to fight the disease and its acceptance. The aim of research was to determine whether and to what extent diabetes modulates the quality of life of patients and in particular which domain of the patient’s life is most limited. In addition, we looked at whether the quality of life perceived by patients is determined by gender and whether and to what extent they accept their illness. Material and methods A study on 100 patients with type 2 diabetes from the Wielkopolska region in Poland was carried out at the end of 2013/beginning of 2014 using a diagnostic survey, and the research techniques were a standardized questionnaire – ADDQoL19 (Audit of Diabetes-Dependent Quality of Life 19) and the AIS questionnaire (Acceptance of Illness Scale) developed by Felton and modified by Juczyński. Results The results suggest that the quality of life of the patients was “neither good nor bad”, whereby women perceive it as being lower than men. While analysing the impact of diabetes on the different domains of the lives of men and women, it should be stressed that most affected were diet, satisfying appetite, independence, financial position, feelings about the future, sex life, and freedom in the consumption of drinks. More than half of men and women did not accept their illness; however, younger persons unlike older accepted diabetes to a much greater degree. Conclusions Type 2 diabetes negatively affects the quality of life of patients and its impact is felt more by women. In both sexes, the most affected domain of quality of life is the lack of nutritional freedom. The acceptance of illness is dependent upon age.
Use and Effectiveness of Quitlines for Smokers With Diabetes: Cessation and Weight Outcomes, Washington State Tobacco Quit Line, 2008

Introduction Having diabetes and smoking increases the risk of morbidity and mortality. However, cessation-related weight gain, a common side effect during quitting, can further complicate diabetes. Evidence-based telephone quitlines can support quitting but have not been studied adequately in populations with chronic diseases such as diabetes. The purpose of this study was to evaluate the use and effectiveness of a tobacco quitline among tobacco users with diabetes. Cessation-related weight concerns and weight gain were also assessed. Methods We administered a telephone-based follow-up survey to tobacco users with and without diabetes 7 months after their enrollment in a quitline. We collected and analyzed data on demographics, tobacco use, dieting, weight concern, quitting success (7- and 30-day point prevalence), and weight gain. We computed summary statistics for descriptive data, χ2 and t tests for bivariate comparisons, and multivariable analyses to determine correlates of cessation. Results Tobacco users with diabetes used the quitline in a greater proportion than they were represented in the general population. Quit rates for those with and without diabetes did not differ significantly (24.3% vs 22.5%). No significant differences existed between groups for weight gain at follow-up, regardless of quit status. However, participants with diabetes reported more weight gain in previous quit attempts (34.2% vs 22.4% gained >20 lbs, P = .03). Weight concern was a significant correlate of continued smoking, regardless of diabetes status. Conclusions Results suggest that quitlines are effective for participants with diabetes, but tailored interventions that address weight concerns during cessation are needed.
Planar Cell Polarity Aligns Osteoblast Division in Response to Substrate Strain

Exposure of bone to dynamic strain increases the rate of division of osteoblasts and also influences the directional organization of the cellular and molecular structure of the bone tissue that they produce. Here, we report that brief exposure to dynamic substrate strain (sufficient to rapidly stimulate cell division) influences the orientation of osteoblastic cell division. The initial proliferative response to strain involves canonical Wnt signaling and can be blocked by sclerostin. However, the strain‐related orientation of cell division is independently influenced through the noncanonical Wnt/planar cell polarity (PCP) pathway. Blockade of Rho‐associated coiled kinase (ROCK), a component of the PCP pathway, prevents strain‐related orientation of division in osteoblast‐like Saos‐2 cells. Heterozygous loop‐tail mutation of the core PCP component van Gogh‐like 2 (Vangl2) in mouse osteoblasts impairs the orientation of division in response to strain. Examination of bones from Vangl2 loop‐tail heterozygous mice by µCT and scanning electron microscopy reveals altered bone architecture and disorganized bone‐forming surfaces. Hence, in addition to the well‐accepted role of PCP involvement in response to developmental cues during skeletal morphogenesis, our data reveal that this pathway also acts postnatally, in parallel with canonical Wnt signaling, to transduce biomechanical cues into skeletal adaptive responses. The simultaneous and independent actions of these two pathways appear to influence both the rate and orientation of osteoblast division, thus fine‐tuning bone architecture to meet the structural demands of functional loading. © 2014 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals, Inc. on behalf of the American Society for Bone and Mineral Research.
Unsaturated flow behavior in double-scale porous reinforcement for liquid composite molding processes

We investigate the unsaturated resin flow behavior in a dual scale porosity preform by observing the pressure distribution and the void content. The experimental data show that the pressure profile in the unsaturated flow is nonlinear with positive curvature whereas that in the saturated flow is linear as expected from the classical Darcy’s law. To address this issue, the governing equation for mass conservation is modified by introducing a mass sink term. Eventually, it has been found that the discrepancy between the unsaturated and saturated permeability values comes from a misinterpretation of the pressure gradient at the flow front in the unsaturated permeability measurement method and the permeability for a given preform is a unique value regardless of measurement method or flow condition. Based on this investigation, the ratio of unsaturated permeability to saturated permeability is represented as a dimensionless number in terms of void content.
Cell Death Pathways Associated with Photodynamic Therapy: An Update

Photodynamic therapy (PDT) has the potential to make a significant impact on cancer treatment. PDT can sensitize malignant tissues to light, leading to a highly selective effect if an appropriate light dose can be delivered. Variations in light distribution and drug delivery, along with impaired efficacy in hypoxic regions, can reduce the overall tumor response. There is also evidence that malignant cells surviving PDT may become more aggressive than the initial tumor population. Promotion of more effective direct tumor eradication is therefore an important goal. While a list of properties for the “ideal” photosensitizing agent often includes formulation, pharmacologic and photophysical elements, we propose that subcellular targeting is also an important consideration. Perspectives relating to optimizing PDT efficacy are offered here. These relate to death pathways initiated by photodamage to particular subcellular organelles.
New Insights for Detecting and Deriving Thermal Properties of Lava Flow Using Infrared Satellite during 2014-2015 Effusive Eruption at Holuhraun, Iceland

A new lava field was formed at Holuhraun in the Icelandic Highlands, north of Vatnajokull glacier, in 2014–2015. It was the largest effusive eruption in Iceland for 230 years, with an estimated lava bulk volume of ~1.44 km3 covering an area of ~84 km2. Satellite-based remote sensing is commonly used as preliminary assessment of large scale eruptions since it is relatively efficient for collecting and processing the data. Landsat-8 infrared datasets were used in this study, and we used dual-band technique to determine the subpixel temperature (Th) of the lava. We developed a new spectral index called the thermal eruption index (TEI) based on the shortwave infrared (SWIR) and thermal infrared (TIR) bands allowing us to differentiate thermal domain within the lava flow field. Lava surface roughness effects are accounted by using the Hurst coefficient (H) for deriving the radiant flux ( Φ rad ) and the crust thickness (Δh). Here, we compare the results derived from satellite images with field measurements. The result from 2 December 2014 shows that a temperature estimate (1096 °C; occupying area of 3.05 m2) from a lava breakout has a close correspondence with a thermal camera measurement (1047 °C; occupying area of 4.52 m2). We also found that the crust thickness estimate in the lava channel during 6 September 2014 (~3.4–7.7 m) compares closely with the lava height measurement from the field (~2.6–6.6 m); meanwhile, the total radiant flux peak is underestimated (~8 GW) compared to other studies (~25 GW), although the trend shows good agreement with both field observation and other studies. This study provides new insights for monitoring future effusive eruption using infrared satellite images.