Miracle-dz/UniFAR-FTUs huggingface dataset:
Evolution of the Sabin Strain of Type 3 Poliovirus in an Immunodeficient Patient during the Entire 637-Day Period of Virus Excretion

ABSTRACT A 20-year-old female hypogammaglobulinemic patient received monotypic Sabin 3 vaccine in 1962. The patient excreted type 3 poliovirus for a period of 637 days without developing any symptoms of poliomyelitis, after which excretion appeared to have ceased spontaneously. The evolution of Sabin 3 throughout the entire period of virus excretion was studied by characterization of seven sequential isolates from the patient. The isolates were analyzed in terms of their antigenic properties, virulence, sensitivity for growth at high temperatures, and differences in nucleotide sequence from the Sabin type 3 vaccine. The isolates followed a main lineage of evolution with a rate of nucleotide substitution that was very similar to that estimated for wild-type poliovirus during person-to-person transmission. There was a delay in the appearance of antigenic variants compared to sequential type 3 isolates from healthy vaccines, which could be one of the possible explanations for the long-term excretion of virus from the patient. The distribution of mutations in the isolates identified regions of the virus possibly involved in adaptation for growth in the human gut and virus persistence. None of the isolates showed a full reversion of the attenuated and temperature-sensitive phenotypes of Sabin 3. Information of this sort will help in the assessment of the risk of spread of virulent polioviruses from long-term excretors and in the design of therapies to stop long-term excretion. This will make an important contribution to the decision-making process on when to stop vaccination once wild poliovirus has been eradicated.
Anticancer, antioxidant activities, and DNA affinity of novel monocationic bithiophenes and analogues

A series of 15 monocationic bithiophenes and isosteres were prepared and subjected to in vitro antiproliferative screening using the full National Cancer Institute (NCI)-60 cell line panel, representing nine types of cancer. Among the nine types of cancer involved in a five-dose screen, non-small cell lung and breast cancer cell lines were the most responsive to the antiproliferative effect of the tested compounds, especially cell lines A549/ATCC, NCI-H322M, and NCI-H460, whereas compounds 1a, 1c, 1d, and 7 exhibited potent activity, with GI50 values (drug concentration that causes 50% inhibition of cell growth) from less than 10 nM to 102 nM. In addition, compounds 1c and 1d gave GI50 values of 73 nM and 79 nM, respectively, against the MDA-MB-468 breast cancer cell line. Structure–activity relationship findings indicated that the mononitriles were far less active than their corresponding monoamidines and, within the amidines series, the bioisosteric replacement of a thiophene ring by a furan led to a reduction in antiproliferative activity. Also, molecular manipulations, involving substitution on the phenyl ring, or its replacement by a pyridyl, or alteration of the position of the amidine group, led to significant alteration in antiproliferative activity. On the other hand, DNA studies demonstrated that these monoamidine bichalcophenes have promising ability to cleave the genomic DNA. These monoamidines show a wide range of DNA affinities, as judged from their DNA cleavage effect, which are remarkably sensitive to all kinds of structural modifications. Finally, the novel bichalcophenes were tested for their antioxidant property by the ABTS (2,2′-azino- bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt) assay, as well as lipid and nitric oxide scavenging techniques, and were found to exhibit good-to-potent antioxidant abilities.
Hydrological gradients and functional diversity of plants drive ecosystem processes in Poyang Lake wetland

Functional trait approaches have greatly improved the mechanistic understanding of the impacts of biodiversity in ecosystem. We evaluated how hydrological gradients (i.e., soil water content and elevation) affected the taxonomic diversity, functional identity, and functional diversity and assessed how changes in these measures of diversity ultimately drove variations in above‐ground biomass (AGB). Results showed that a combination of hydrological gradients and functional trait composition (i.e., the community weighted means, the single‐trait functional diversity and the multiple‐trait functional diversity) better explained variations (40.8%) of AGB. No significant relationships between taxonomic diversity and AGB were observed. The dominant species became taller with thicker stem, and co‐occurring species showed greater divergent distribution of stem diameter towards higher soil water content. As elevation increased, the dominant plant had thinner leaves with later onset of flowering and longer flowering duration, and co‐occurring species showed less divergent distribution of lamina thickness and leaf dry‐mass content. Our results highlighted that the positive biodiversity–ecosystem functioning relations were largely attributed to the co‐operations of selection and complementary effects, as evidences showed that the dominant trait values (i.e., community weighted mean of shoot height) and trait diversity (i.e., functional evenness) improved biomass production of wetland plants.
Generalized Additive Models with Implicit Variable Selection by Likelihood-Based Boosting G. Tutz and H. Binder

We examine the GAMBoost method and R package of Tutz and Binder (2006), and its eﬀectiveness. Whilst in many examples the algorithm performs relatively well, we ﬁnd signiﬁcant diﬃculties with the approach taken, particularly in terms of computational time, automatic smoothing parameter selection and the claimed ‘implicit’ variable selection. We also ﬁnd that GAMBoost performs particularly poorly in cases of high signal-to-noise ratio.
Selectivity assessment of chlorfenvinphos reevaluated by including physiological and behavioral effects on an important beneficial insect

Selectivity is an important factor in identifying candidate pesticides to be used in crop protection since it characterizes chemicals that, while being effective against target pests, exert an acceptable impact on the other components of the environment. Extrapolated to an integrated pest management (IPM) context, selectivity implies that candidate pesticides may preserve the ability of beneficial insects to significantly control target pest populations. In the present study, we assess the physiological selectivity of the organophosphate chlorfenvinphos, used to protect cruciferous crops against the cabbage root fly, Delia radicum (Diptera: Anthomyiidae), by investigating both the lethal and sublethal effects exerted on its main parasitoid Trybliographa rapae (Hymenoptera: Figitidae). The comparison of the median lethal doses showed that T. rapae was at least seven times less sensitive than D. radicum to chlorfenvinphos. However, longevity of parasitoids surviving a sublethal dose was reduced by half. The potential fecundity of females was decreased by 9.6 to 22.8%. Chlorfenvinphos also induced important behavioral changes in both sexes and reduced the chances for parasitoids to mate by more than 70%. While most behavioral changes were reversible, effects on mating and on fecundity were not, thereby suggesting long‐term effects on the reproduction of the parasitoid. These cumulative effects of chlorfenvinphos would have dramatic consequences on the efficacy of parasitoids contacting such doses of chlorfenvinphos in the field and therefore there is question about the intrinsic selectivity of this insecticide.
Towards estimating nadir objective vector using evolutionary approaches

Nadir point plays an important role in multi-objective optimization because of its importance in estimating the range of objective values corresponding to desired Pareto-optimal solutions and also in using many classical interactive optimization techniques. Since this point corresponds to the worst Pareto-optimal solution of each objective, the task of estimating the nadir point necessitates information about the whole Pareto optimal frontier and is reported to be a difficult task using classical means. In this paper, for the first time, we have proposed a couple of modifications to an existing evolutionary multi-objective optimization procedure to focus its search towards the extreme objective values front-wise. On up to 20-objective optimization problems, both proposed procedures are found to be capable of finding a near nadir point quickly and reliably. Simulation results are interesting and should encourage further studies and applications in estimating the nadir point, a process which should lead to a better interactive procedure of finding and arriving at a desired Pareto-optimal solution.
Longitudinal Analysis of Android Ad Library Permissions

This paper investigates changes over time in the behavior of Android ad libraries. Taking a sample of 100,000 apps, we extract and classify the ad libraries. By considering the release dates of the applications that use a specific ad library version, we estimate the release date for the library, and thus build a chronological map of the permissions used by various ad libraries over time. We find that the use of most permissions has increased over the last several years, and that more libraries are able to use permissions that pose particular risks to user privacy and security.
Altered Atrial Metabolism: An Underappreciated Contributor to the Initiation and Progression of Atrial Fibrillation

Atrial fibrillation (AF) is the most common cardiac arrhythmia and is associated with increased morbidity and mortality. Currently, about 1% to 2% of the general population suffers from AF. [1][1] The incidence and prevalence of AF increase in an age‐dependent manner, resulting in a projected AF‐
Supermassive black holes in the Sbc spiral galaxies NGC 3310, NGC 4303 and NGC 4258 ,

We have undertaken an HST Space Telescope Imaging Spectrograph survey of 54 late type spiral galaxies to study the scalin g relations between black holes and their host spheroids at the low mass end. Our aim is to measure black hole masses or to set upper limits for a sizeable sample of spiral galaxies. In this paper we present new Space Telescope Imaging Spectrograph (STIS) observations of three spiral galaxies, NGC 4303, NGC 3310 and NGC 4258. The bright optical emission lines Hα λ 6564A, [NII]λλ 6549, 6585A and [SII]λλ 6718, 6732A were used to study the kinematics of the ionized gas in the nuclear region of each galaxy with a∼ 0.07 ′′ spatial resolution. Our STIS data for NGC 4258 were analyzed in conjunction with archival ones to compare the gas kinematical estimate of the black hole mass with the accurate value from H20-maser observations. In NGC 3310, the observed gas kinematics is well matched by a circularly rotating disk model but we are only able to set an upper limit to the BH mass which, taking into account the allowed disk inclinations, varies in the range 5.0× 10 6 − 4.2× 10 7 M⊙ at the 95% confidence level. In NGC 4303 the kinematical data require the presence of a BH with mass MBH = (5.0) +0.87
Rfm1, a Novel Tethering Factor Required To Recruit the Hst1 Histone Deacetylase for Repression of Middle Sporulation Genes

ABSTRACT Transcriptional repression is often correlated with the alteration of chromatin structure through modifications of the nucleosomes in the promoter region, such as by deacetylation of the N-terminal histone tails. This is presumed to make the promoter region inaccessible to other regulatory factors and the general transcription machinery. To accomplish this, histone deacetylases are recruited to specific promoters via DNA-binding proteins and tethering factors. We have previously reported the requirement for the NAD+-dependent histone deacetylase Hst1 and the DNA-binding protein Sum1 for vegetative repression of many middle sporulation genes in Saccharomyces cerevisiae. Here we report the identification of a novel tethering factor, Rfm1, that is required for Hst1-mediated repression. Rfm1 interacts with both Sum1 and Hst1 and is required for the Sum1-Hst1 interaction. DNA microarray and Northern blot analyses showed that Rfm1 is required for repression of the same subset of Sum1-repressed genes that require Hst1. These results suggest that Rfm1 is a specificity factor that targets the Hst1 deacetylase to a subset of Sum1-regulated genes.
Treatment with CSII in two infants with neonatal diabetes mellitus

This article reports the case studies of two children with neonatal onset of diabetes who were treated with continuous subcutaneous insulin infusion (CSII) from within 4 d to 3 wk of the diagnosis. The aim was to describe diabetes‐related and insulin‐pump‐specific data in relation to growth and various feeding patterns when using CSII in infants with diabetes during their first year of life. The two children’s medical records were scrutinized. The results showed that both children had good metabolic control [median hemoglobin A1c (HbA1c) 5.3 and 5.7%, high performance liquid chromatography (HPLC) method, reference: 3.4–5.0%. Compared with the Diabetes Control and Complications Trial (DCCT) HbA1c units, Swedish units give approximately 1% point lower results]. No episodes of severe hypoglycemia or diabetic ketoacidosis have been demonstrated. The children had normal growth patterns, as they followed a normal feeding regime for their age. The meal doses of insulin were given over 12 min to 3 h. The children had diluted Humalog® insulin 10 U/mL (Eli Lilly & Co, Indianapolis, IN, USA) in their pumps. Different types of insulin pumps were used, namely, the Minimed 507C and 508 (Medtronic, Minneapolis, MN, USA), and a Disetronic H‐tron V100 (Roche Diagnostics, Basel, Switzerland). The children used different types of infusion sets. Neither family reported any technical problems with their pump system. CSII was an effective and safe treatment for the two children suffering from neonatal diabetes. This offers an alternative for other infants with a similar diagnosis.
Routine HIV Testing among Providers of HIV Care in the United States, 2009

In 2006, CDC recommended HIV screening as part of routine medical care for all persons aged 13–64 years. We examined adherence to the recommendations among a sample of HIV care providers in the US to determine if known providers of HIV care are offering routine HIV testing in outpatient settings. Data were from the CDC's Medical Monitoring Project Provider Survey, administered to physicians, nurse practitioners and physician assistants from June-September 2009. We assessed bivariate associations between testing behaviors and provider and practice characteristics and used multivariate regression to determine factors associated with offering HIV screening to all patients aged 13–64 years. Sixty percent of providers reported offering HIV screening to all patients 13 to 64 years of age. Being a nurse practitioner (aOR = 5.6, 95% CI = 2.6–11.9) compared to physician, age<39 (aOR = 1.9, 95% CI = 1.0–3.5) or 39–49 (aOR = 2.1, 95% CI = 1.4–3.3) compared with ≥50 years, and black race (aOR = 2.6, 95% CI = 1.2–6.0) compared with white race was associated with offering testing to all patients. Providers with low (aOR = 0.2, 95% CI = 0.1–0.3) or medium (aOR = 0.4, 95% CI = 0.2–0.6) HIV-infected patient loads were less likely to offer HIV testing to all patients compared with providers with high patient loads. Many providers of HIV care are still conducting risk-based rather than routine testing. We found that provider profession, age, race, and HIV-infected patient load were associated with offering HIV testing. Health care providers should use patient encounters as an opportunity to offer routine HIV testing to patients as outlined in CDC's revised recommendations for HIV testing in health care settings.
Role of Lymphocytes in Myocardial Injury, Healing, and Remodeling After Myocardial Infarction

A large body of evidence produced during decades of research indicates that myocardial injury activates innate immunity. On the one hand, innate immunity both aggravates ischemic injury and impedes remodeling after myocardial infarction (MI). On the other hand, innate immunity activation contributes to myocardial healing, as exemplified by monocytes’ central role in the formation of a stable scar and protection against intraventricular thrombi after acute infarction. Although innate leukocytes can recognize a wide array of self-antigens via pattern recognition receptors, adaptive immunity activation requires highly specific cooperation between antigen-presenting cells and distinct antigen-specific receptors on lymphocytes. We have only recently begun to examine lymphocyte activation’s relationship to adaptive immunity and significance in the context of ischemic myocardial injury. There is some experimental evidence that CD4+ T-cells contribute to ischemia–reperfusion injury. Several studies have shown that CD4+ T-cells, especially CD4+ T-regulatory cells, improve wound healing after MI, whereas depleting B-cells is beneficial post MI. That T-cell activation after MI is induced by T-cell receptor signaling implicates autoantigens that have not yet been identified in this context. Also, the significance of lymphocytes in humans post MI remains unclear, primarily as a result of methodology. This review summarizes current experimental evidence of lymphocytes’ activation, functional role, and crosstalk with innate leukocytes in myocardial ischemia–reperfusion injury, wound healing, and remodeling after myocardial infarction.
The VISA-A questionnaire: a valid and reliable index of the clinical severity of Achilles tendinopathy

Background—There is no disease specific, reliable, and valid clinical measure of Achilles tendinopathy. Objective—To develop and test a questionnaire based instrument that would serve as an index of severity of Achilles tendinopathy. Methods—Item generation, item reduction, item scaling, and pretesting were used to develop a questionnaire to assess the severity of Achilles tendinopathy. The final version consisted of eight questions that measured the domains of pain, function in daily living, and sporting activity. Results range from 0 to 100, where 100 represents the perfect score. Its validity and reliability were then tested in a population of non-surgical patients with Achilles tendinopathy (n = 45), presurgical patients with Achilles tendinopathy (n = 14), and two normal control populations (total n = 87). Results—The VISA-A questionnaire had good test-retest (r = 0.93), intrarater (three tests, r = 0.90), and interrater (r = 0.90) reliability as well as good stability when compared one week apart (r = 0.81). The mean (95% confidence interval) VISA-A score in the non-surgical patients was 64 (59–69), in presurgical patients 44 (28–60), and in control subjects it exceeded 96 (94–99). Thus the VISA-A score was higher in non-surgical than presurgical patients (p = 0.02) and higher in control subjects than in both patient populations (p<0.001). Conclusions—The VISA-A questionnaire is reliable and displayed construct validity when means were compared in patients with a range of severity of Achilles tendinopathy and control subjects. The continuous numerical result of the VISA-A questionnaire has the potential to provide utility in both the clinical setting and research. The test is not designed to be diagnostic. Further studies are needed to determine whether the VISA-A score predicts prognosis.
Low‐intensity resistance exercise with slow lifting and lowering does not increase noradrenalin and cardiovascular responses

The aim of this study was to investigate the effect of low‐intensity resistance exercise with slow lifting and lowering (LSL) on plasma endothelin‐1 (ET‐1) and noradrenalin concentrations in young healthy adults. Eight healthy males participated in this study (age 19·0 ± 0·5 years, mean ± SD). The LSL performed the 10 repetitions with 3 s eccentric (lowering phase) and 3 s concentric (lifting phase) muscle actions. The high‐intensity resistance exercise with normal lifting and lowering (HNL) performed the 10 repetitions with 1 s eccentric (lowering phase) and 1 s concentric (lifting phase) muscle actions. The load was set to 40% of one repetition maximal (1RM) for LSL and 80% of 1RM for HNL. Plasma ET‐1 and noradrenalin concentrations were measured before and after each type of exercise. Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), pulse pressure (PP) and heart rate (HR) during LSL and HNL were measured. The rate‐pressure product (RPP) was calculated from SBP and HR. There were no significant differences in the plasma ET‐1 concentration between LSL and HNL. However, the plasma noradrenalin concentration was significantly increased after HNL, compared with LSL (P<0·001). SBP, DBP, PP, MAP, HR and RPP during LSL were significantly lower compared with HNL (P<0·05: PP and HR; P<0·01: RPP; P<0·001: SBP, DBP and MAP). These results suggested that LSL may suppress the increase in plasma noradrenalin concentrations and cardiovascular responses.
Recognizing Predatory Chat Documents using Semi-supervised Anomaly Detection

Chat-logs are informative documents available to nowadays social network providers. Providers and law enforcement tend to use these huge logs anonymously for automatic online Sexual Predator Identification (SPI) which is a relatively new area of application. The task plays an important role in protecting children and juveniles against being exploited by online predators. Pattern recognition techniques facilitate automatic identification of harmful conversations in cyber space by law enforcements. These techniques usually require a large volume of high-quality training instances of both predatory and non-predatory documents. However, collecting non-predatory documents is not practical in real-world applications, since this category contains a large variety of documents with many topics including politics, sports, science, technology and etc. We utilized a new semi-supervised approach to mitigate this problem by adapting an anomaly detection technique called One-class Support Vector Machine which does not require non-predatory samples for training. We compared the performance of this approach against other state-of-the-art methods which use both positive and negative instances. We observed that although anomaly detection approach utilizes only one class label for training (which is a very desirable property in practice); its performance is comparable to that of binary SVM classification. In addition, this approach outperforms the classic two-class Naïve Bayes algorithm, which we used as our baseline, in terms of both classification accuracy and precision.
Abacavir/3TC vs. tenofovir/FTC: interim results from ACTG 5202.

On February 28, 2008, the National Institute of Allergy and Infectious Diseases (NIAID) reported that the protocol for ACTG 5202 is being modified because of reduced virologic response in a subset of patients who were receiving abacavir/3TC. This highly anticipated, phase III, multicenter trial involves 1858 HIV-infected adults in the U.S. who were randomized to begin antiretroviral therapy with one of four regimens: The comparison between NRTI backbones is double-blinded, with patients taking a placebo pill for whichever NRTI combination they are not receiving. The comparison between efavirenz …