Miracle-dz/UniFAR-FTUs huggingface dataset:
The Positive Regulatory Roles of the TIFY10 Proteins in Plant Responses to Alkaline Stress

The TIFY family is a novel plant-specific protein family, and is characterized by a conserved TIFY motif (TIFF/YXG). Our previous studies indicated the potential roles of TIFY10/11 proteins in plant responses to alkaline stress. In the current study, we focused on the regulatory roles and possible physiological and molecular basis of the TIFY10 proteins in plant responses to alkaline stress. We demonstrated the positive function of TIFY10s in alkaline responses by using the AtTIFY10a and AtTIFY10b knockout Arabidopsis, as evidenced by the relatively lower germination rates of attify10a and attify10b mutant seeds under alkaline stress. We also revealed that ectopic expression of GsTIFY10a in Medicago sativa promoted plant growth, and increased the NADP-ME activity, citric acid content and free proline content but decreased the MDA content of transgenic plants under alkaline stress. Furthermore, expression levels of the stress responsive genes including NADP-ME, CS, H+-ppase and P5CS were also up-regulated in GsTIFY10a transgenic plants under alkaline stress. Interestingly, GsTIFY10a overexpression increased the jasmonate content of the transgenic alfalfa. In addition, we showed that neither GsTIFY10a nor GsTIFY10e exhibited transcriptional activity in yeast cells. However, through Y2H and BiFc assays, we demonstrated that GsTIFY10a, not GsTIFY10e, could form homodimers in yeast cells and in living plant cells. As expected, we also demonstrated that GsTIFY10a and GsTIFY10e could heterodimerize with each other in both yeast and plant cells. Taken together, our results provided direct evidence supporting the positive regulatory roles of the TIFY10 proteins in plant responses to alkaline stress.
Simulation of Austenite-to-ferrite Transformation in Deformed Austenite by Crystal Plasticity Finite Element Method and Multi-phase-field Method

1) Graduate School of Science and Engineering, Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo, 1528552 Japan. E-mail: ayamanaka@mes.titech. ac.jp 2) Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585 Japan. E-mail: takaki@kit.ac.jp 3) Faculty of Engineering, Fukui University of Technology, 3-6-1, Gakuen, Fukui, 910-8505 Japan. E-mail: tomita@fukui-ut.ac.jp
Role of Balloon Dilation in the Management of Adult Idiopathic Subglottic Stenosis

Objectives We evaluated the efficacy of balloon dilation for adjunctive and symptomatic management of isolated idiopathic subglottic stenosis in adults. Methods Adults with airway obstruction symptoms classified as idiopathic subglottic stenosis based on history and findings of a single discrete stenotic area on microlaryngoscopy and bronchoscopy were included in this series. Patients who met these criteria underwent dilation with a 10- to 14-mm balloon in a single procedure or in 2 consecutive dilations within 7 days. The patients were followed for up to 30 months after dilation. Results Six patients met the criteria. One of the 6 had prior laser treatments and a cricotracheal resection. One patient had a previous scar band lysis procedure. The remaining 4 patients had no prior procedures. The airway sizes prior to dilation ranged from a 2.5 endotracheal tube to a 5.0 endotracheal tube. In all cases the airway was dilated to 2.0 to 3.5 endotracheal tube sizes larger than the initial size. To date, 4 patients have been followed for 10 to 30 months without symptoms of recurrent airway stenosis. One patient was symptom-free for 22 months, then presented with progressive airway difficulty following an upper respiratory tract infection, and has undergone a repeat dilation. No patients had adverse effects or complications from the procedure. Conclusions Balloon dilation of idiopathic subglottic stenosis in adults is a relatively safe and effective method to manage this disease entity for cases of isolated and discrete lesions. Patients who underwent a single procedure have remained symptom-free for up to 30 months after balloon dilation.
Mapping-chains for Studying Concept Shift in Political Ontologies

For some years now ontologies have been used in Social Science, e.g., in annotation of newspaper articles for disambiguating concepts within Media Analysis. These ontologies and annotations have now become objects of study in their own right, as they implicitly represent the shift of meaning of political concept over time. Manual mappings, which are intrinsically intensional, can hardly capture such subtle changes, but we claim that automatic instance-based mappings, with their extensional character, are more suitable for producing interesting mapping-chains. In this paper, we evaluate the use of instance-based ontology mappings for producing concept chains in a case-study in Communication Science on a corpus with ontologies describing the Dutch election campaigns since 1994. This initial research shows the potential of the associative character of extensional mapping-chains, but also indicates a number of unsolved open questions, most significantly the lack of a proper methodology for evaluating such chains due to the open, explorative character of the task.
Effects of subject velocity on ground reaction force measurements and stance times in clinically normal horses at the walk and trot.

OBJECTIVE The objective of the study reported here was to evaluate the effects of changing velocity on stance time and ground reaction force (GRF) measurements in horses at the walk and trot. DESIGN Force plate gait analysis was used to evaluate clinically normal horses at variable velocities. Ground reaction force measurements and stance times were recorded and compared. ANIMALS 12 adult horses. PROCEDURE Data were obtained from 192 valid trials at the walk and 162 valid trials at the trot. Vertical, braking, and propulsive peak forces and impulses were measured. Pearson's correlation coefficients were determined for velocity and stance time and all measured forces and impulses in the forelimbs and hind limbs. Trials were divided into distinct velocity ranges. Trials obtained at velocities within the established ranges were analyzed to evaluate changes in vertical, braking, and propulsive peak forces and impulses at differing speeds within the walk and trot gaits. RESULTS At the walk and trot, a significant negative correlation was found between velocity and forelimb and hind limb stance times. Velocity and stance time were significantly correlated with many of the GRF and impulse measurements. Velocity was significantly correlated with vertical and braking forces in the hind limbs at the walk, with vertical force in the forelimbs at the trot, and with braking force in the forelimbs and hind limbs at the trot. Velocity and stance time correlated significantly with forelimb and hind limb vertical impulses. Forelimb and hind limb stance times decreased significantly as velocity increased. Hind limb braking force increased and forelimb and hind limb vertical impulses decreased significantly as walk velocity increased. Forelimb braking force increased significantly between velocity ranges at the trot. CONCLUSIONS Results of this study confirm that a significant negative linear correlation exists between subject velocity and stance times in clinically normal horses at the walk and trot. Significant correlations were also identified between velocity and many GRF measurements, indicating that subject velocity does influence the generation of GRF measurements in horses. Variation in subject velocity should be minimized when performing force-plate analysis in horses.
Laparoscopic radical adrenalectomy for malignancy in 31 patients.

PURPOSE Laparoscopic adrenalectomy for malignancy is controversial. We analyzed our experience with laparoscopic radical adrenalectomy for cancer with an emphasis on predictors of surgical outcome and oncological followup data. MATERIALS AND METHODS Since July 1997, 31 patients have undergone a total of 33 laparoscopic adrenalectomies for malignancy. Mean adrenal tumor size was 5 cm (range 1.8 to 9). The laparoscopic approach was transperitoneal in 17 cases, retroperitoneal in 15 and transthoracic in 1. Data were obtained from patient charts, radiographic reports and direct telephone calls to patient families. RESULTS Associated organ resection (radical nephrectomy) was performed in 3 patients. One case was electively converted to open surgery. There was no operative mortality. The pathological diagnoses were metastatic cancer in 26 cases and primary adrenal malignancy in 7. Current median followup, available on 30 patients, was 26 months (range 1 to 69). Overall 15 patients (48%) died and 16 (52%) were alive, of whom 13 (42%) showed no evidence of disease. Cancer specific survival at a median followup of 42 months was 53% and 5-year actuarial survival was 40%. Local recurrence was noted in 7 patients (23%). There were no port site metastases. Survival was similar in patients with tumors less than 5 cm vs 5 cm or greater. Survival was not associated with patient age, tumor size, operative time or surgical approach. Survival was compromised in patients with local recurrence (p = 0.016). CONCLUSIONS Laparoscopic radical adrenalectomy can be performed with acceptable outcomes in the carefully selected patient with a small, organ confined, solitary adrenal metastasis or primary adrenal carcinoma. To our knowledge the largest series in the literature to date is presented.
Structure changes caused by strain annealing of nylon 6 fibers

Abstract Experiments are described in which nylon 6 fibers are annealed while subjected to a constant stretch (or slack). Subsequent mechanical and structural measurements are described and analyzed. A paracrystalline structure model is proposed in which folded chains, fully extended chains, partially folded, and partially extended chains coexist in the highly drawn high strength fiber. An explanation of structural changes occurring during the thermal-mechanical treatment is that the folded and partially folded chains are arranged randomly in staggered fashion in small units throughout the structure. During slack annealing, the chains become more folded and shrinkage occurs. Some of the chain refolding will be permanent and may act as new defect sites thereby reducing fracture stress. During annealing in the presence of comparatively high tensile stresses the folded chains are unfolded to some extent, but not completely, and the load-carrying chains in the structure are more uniformly loaded. At the highe.
The eye and the heart

The vasculature of the eye and the heart share several common characteristics. The easily accessible vessels of the eye are therefore—to some extent—a window to the heart. There is interplay between cardiovascular functions and risk factors and the occurrence and progression of many eye diseases. In particular, arteriovenous nipping, narrowing of retinal arteries, and the dilatation of retinal veins are important signs of increased cardiovascular risk. The pressure in the dilated veins is often markedly increased due to a dysregulation of venous outflow from the eye. Besides such morphological criteria, functional alterations might be even more relevant and may play an important role in future diagnostics. Via neurovascular coupling, flickering light dilates capillaries and small arterioles, thus inducing endothelium-dependent, flow-mediated dilation of larger retinal vessels. Risk factors for arteriosclerosis, such as dyslipidaemia, diabetes, or systemic hypertension, are also risk factors for eye diseases such as retinal arterial or retinal vein occlusions, cataracts, age-related macular degeneration, and increases in intraocular pressure (IOP). Functional alterations of blood flow are particularly relevant to the eye. The primary vascular dysregulation syndrome (PVD), which often includes systemic hypotension, is associated with disturbed autoregulation of ocular blood flow (OBF). Fluctuation of IOP on a high level or blood pressure on a low level leads to instable OBF and oxygen supply and therefore to oxidative stress, which is particularly involved in the pathogenesis of glaucomatous neuropathy. Vascular dysregulation also leads to a barrier dysfunction and thereby to small retinal haemorrhages.
Tympano‐Ossiculoplasty Utilizing the Spiggle and Theis Titanium Total Ossicular Replacement Prosthesis

Objective Since 1993, titanium prostheses have been used in Europe as a biocompatible implant for ossicular reconstruction. More recently, the titanium ossicular prosthesis has become more widely available in the United States as a newer means of surgical restoration of hearing. We will review the hearing results of patients who have undergone tympano‐ossiculoplasty using a titanium total ossicular replacement prosthesis (TOP). We will compare these results to our previously published results using the porous polyethylene TOP.
Automaticity and Entrance Block Induced by Focal Depolarization of Mammalian Ventricular Tissues

Isolated canine interventricular septa were studied with standard microelectrode tech-niques. Focal automaticity was induced by applying depolarizing current through an extracellular pipet in contact with the right bundle branch (RBB) of the ventricular specialized conducting system. Automaticity appeared with depolarization to transmembrane potentials of −50 mV or less. The spontaneous activity was neither depressed nor accelerated when overdrive suppression was at-tempted. Activity originating within the focus propagated into fully polarized surrounding tissue. However, entrance block, phasically related to the spontaneous cycle length, was an intrinsic property of these foci. Early premature beats initiated outside the focus failed to enter the focus, but the resulting electrotonus delayed the next automatic beat. Late premature beats captured and thereby accelerated the focus. Thus, the automatic foci could be entrained by extrafocal activity. Consequently, continuous pacing at various rates precipitated complex rhythms with fixed coupling. Similar foci with exit conduction, entrance block, and electrotonic modulation also were demonstrated in focally depolarized papillary muscles in feline septal preparations. The unique properties of focally depolarized areas in which spontaneous activity is generated at low membrane potentials provide a mechanism capable of generating a wide array of arrhythmias. Circ Res 47: 238-248, 1980
Guideline-based development of quality indicators for subfertility care.

BACKGROUND Internationally, several organizations have developed clinical guidelines for subfertility care to supply patients with the best possible care. However, to improve the implementation of such guidelines, we first need to gain insight into the application of clinical guidelines in daily practice. Valid quality indicators are necessary to estimate actual guideline adherence. However, none of the existing subfertility guideline programmes is accompanied by a satisfactory set of quality indicators. In this study, we develop a set of valid guideline-based quality indicators for subfertility care. METHODS A systematic RAND-modified Delphi method was used to develop a set of key recommendations based on 10 national Dutch subfertility guidelines, international literature and existing international indicators. Experts' opinions were used to appraise recommendations regarding specific criteria such as efficacy, level of health gain, applicability and potential for care improvement. RESULTS A representative set of 39 key recommendations was selected from 303 initial recommendations. The recommendations covered two structural and 37 procedural aspects, the latter encompassing 'indications for treatment', 'diagnostic procedures', 'treatment procedures' and 'patient information'. CONCLUSIONS This study describes the systematic, stepwise method used to develop 39 process and structure indicators that can be used to monitor subfertility care.
Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling

Significance Protein palmitoylation has been proposed to mediate the recruitment of signaling proteins into lipid rafts. However, a significant shortcoming of this hypothesis is that the proposed kinetics of protein palmitoylation/depalmitoylation heretofore have been too slow to mediate very rapid signaling events. Using a combination of a highly sensitive palmitoylation assay with pulsed metabolic labeling, we were able to detect a population of the Src kinase Lck undergoing extremely rapid palmitoylation and depalmitoylation subsequent to Fas receptor stimulation. The rapid rise and fall in Lck palmitoylation was highly synchronized with other signaling events occurring during the first minutes after activation of the Fas pathway, suggesting the existence of a previously undescribed signal transduction mechanism. Palmitoylation is the posttranslational modification of proteins with a 16-carbon fatty acid chain through a labile thioester bond. The reversibility of protein palmitoylation and its profound effect on protein function suggest that this modification could play an important role as an intracellular signaling mechanism. Evidence that palmitoylation of proteins occurs with the kinetics required for signal transduction is not clear, however. Here we show that engagement of the Fas receptor by its ligand leads to an extremely rapid and transient increase in palmitoylation levels of the tyrosine kinase Lck. Lck palmitoylation kinetics are consistent with the activation of downstream signaling proteins, such as Zap70 and PLC-γ1. Inhibiting Lck palmitoylation not only disrupts proximal Fas signaling events, but also renders cells resistant to Fas-mediated apoptosis. Knockdown of the palmitoyl acyl transferase DHHC21 eliminates activation of Lck and downstream signaling after Fas receptor stimulation. Our findings demonstrate highly dynamic Lck palmitoylation kinetics that are essential for signaling downstream of the Fas receptor.
Evolutionary mechanisms for loneliness

Robert Weiss (1973) conceptualised loneliness as perceived social isolation, which he described as a gnawing, chronic disease without redeeming features. On the scale of everyday life, it is understandable how something as personally aversive as loneliness could be regarded as a blight on human existence. However, evolutionary time and evolutionary forces operate at such a different scale of organisation than we experience in everyday life that personal experience is not sufficient to understand the role of loneliness in human existence. Research over the past decade suggests a very different view of loneliness than suggested by personal experience, one in which loneliness serves a variety of adaptive functions in specific habitats. We review evidence on the heritability of loneliness and outline an evolutionary theory of loneliness, with an emphasis on its potential adaptive value in an evolutionary timescale.
Demonstration of host resistance against isotransplantation of lymphomas induced by the Gross agent.

Summary It was found that a state of relative resistance can be built up in highly inbred skin-compatible C3H mice against the isotransplantation of lymphomas recently induced in the same strain by the Gross virus. Resistance could be built up in two different ways: by pretreating the recipients with homografts of other Gross lymphomas, induced in genetically incompatible mice, or with sub-threshold doses of cells from the same isologous lymphoma. The resistance was relatively weak in comparison with the previously demonstrated antigenicity of methylcholanthrene-induced sarcomas and polyoma-induced tumors. There appears to be partial or complete cross-resistance between different lymphomas induced by the Gross virus.
T‐Cell lymphoblastic lymphoma presenting as bilateral multinodular breast masses: A case report and review of the literature

Non‐Hodgkin lymphoma of T‐cell lineage involving the breast is rare. We report on a 41‐year‐old woman with T‐cell lymphoblastic lymphoma who presented with multiple bilateral breast masses. The patient was treated with intensive chemotherapy and mediastinal and whole‐brain irradiation. She remains in complete remission 24 months after diagnosis. The clinical, histologic, phenotypic, and cytogenetic features are described, with a review of the literature. Am. J. Hematol. 80:216–222, 2005. © 2005 Wiley‐Liss, Inc.
A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A

Ancylostoma ceylanicum, A. caninum, and Giardia lamblia assemblage A are common intestinal parasites of dogs and cats; they can also infect humans, causing parasitic zoonoses. In this study, a multiplex PCR method was developed for simultaneous identification and detection of those three zoonotic parasites. Three pairs of specific primers were designed based on ITS sequence of A. ceylanicum and A. caninum and TPI gene of G. lamblia available in the GenBank. The multiplex PCR reaction system was established by optimizing the reaction condition, and a series of tests on the sensitivity, specificity, and clinical application were also conducted. Results showed that three target fragments were amplified specifically; the detection limit was 10 eggs for both A. ceylanicum and A. caninum, 72 pg DNA for G. lamblia. Of 112 clinical fecal samples, 34.8% and 17.8% samples were positive for A. caninum and A. ceylanicum, respectively, while only 2.7% samples were positive for G. lamblia assemblage A. It is concluded that the established multiplex PCR assay is a convenient, rapid, cost-effective, and high-efficiency method for molecular detection and epidemiological investigation of three zoonotic parasites.
Perceived usefulness, ease of use, and usage of citation database interfaces: a replication

Purpose – This paper aims to report on an investigation into ways in which end‐users perceived citation database interfaces, especially citation database interfaces' usability.Design/methodology/approach – The investigation used the Technology Acceptance Model (TAM) constructs of usefulness and ease of use to assess acceptance of citation database interfaces by university graduate students. A structural equation model was used to fit and validate the Citation Database Interface Acceptance Model (CDIAM). Findings – Causal relationships between the constructs considered by the CDIAM were well supported, accounting for 95 per cent of total variance in citation database interface acceptance and usage. The study concluded that perceived usefulness, and not ease of use of citation database interface, is a key determinant of their acceptance and usage. The results of the construct measurement for perceived usefulness and ease of use in this research are quite consistent with the results reported in recent researc.