Miracle-dz/UniFAR-FTUs huggingface dataset:
PalC, One of Two Bro1 Domain Proteins in the Fungal pH Signalling Pathway, Localizes to Cortical Structures and Binds Vps32

PalC, distantly related to Saccharomyces cerevisiae peripheral endosomal sorting complexes required for transport III (ESCRT‐III) component Bro1p and one of six Aspergillus nidulans pH signalling proteins, contains a Bro1 domain. Green fluorescent protein (GFP)‐tagged PalC is recruited to plasma membrane‐associated punctate structures upon alkalinization, when pH signalling is active. PalC recruitment to these structures is dependent on the seven transmembrane domain (7‐TMD) receptor and likely pH sensor PalH. PalC is a two‐hybrid interactor of the ESCRT‐III Vps20/Vps32 subcomplex and binds Vps32 directly. This binding is largely impaired by Pro439Phe, Arg442Ala and Arg442His substitutions in a conserved region mediating interaction of Bro1p with Vps32p, but these substitutions do not prevent cortical punctate localization, indicating Vps32 independence. In contrast, Arg442Δ impairs Vps32 binding and prevents PalC‐GFP recruitment to cortical structures. pH signalling involves a plasma membrane complex including the 7‐TMD receptor PalH and the arrestin‐like PalF and an endosomal membrane complex involving the PalB protease, the transcription factor PacC and the Vps32 binding, Bro1‐domain‐containing protein PalA. PalC, which localizes to cortical structures and can additionally bind a component of ESCRT‐III, has the features required to bridge these two entities. A likely S. cerevisiae orthologue of PalC has been identified, providing the basis for a unifying hypothesis of gene regulation by ambient pH in ascomycetes.
Lipid Peroxidation and Oxidative Status Compared in Workers at a Bottom Ash Recovery Plant and Fly Ash Treatment Plants

Lipid Peroxidation and Oxidative Status Compared in Workers at a Bottom Ash Recovery Plant and Fly Ash Treatment Plants: Hung‐Shin Liu, et al. Department of Occupational Safety and Health, Chung Shan Medical University, Taiwan—Fly ash and ambient emissions of municipal solid waste incinerators contain polychlorinated dibenzo‐p‐dioxins and dibenzofurans (PCDD/Fs), polycyclic aromatic hydrocarbons (PAHs), other organic compounds, metals, and gases. Hazardous substances such as PCDD/Fs, mercury vapors and other silicates, and the components of bottom ash and fly ash elevate the oxidative damage. We compared oxidative damage in workers exposed to hazardous substances at a bottom ash recovery plant and 3 fly ash treatment plants in Taiwan by measuring their levels of plasma malondialdehyde (MDA) and urine 8‐hydroxydeoxyguanosine (8‐OH‐dG). Significantly higher MDA levels were found in fly ash treatment plant workers (3.20 µM) than in bottom ash plant workers (0.58 µM). There was a significant association between MDA levels in workers and their working environment, especially in the fly ash treatment plants. Levels of 8OH‐dG varied more widely in bottom ash workers than in fly ash workers. The association between occupational exposure and 8‐OH‐dG levels may be affected by the life style of the workers. Because more dioxins and metals may leach from fly ash than from bottom ash, fly ash treatment plant workers should, as much as possible, avoid exposing themselves to fly ash.
Using context to improve emotion detection in spoken dialog systems

Most research that explores the emotional state of users of spoken dialog systems does not fully utilize the contextual nature that the dialog structure provides. This paper reports results of machine learning experiments designed to automatically classify the emotional state of user turns using a corpus of 5,690 dialogs collected with the “How May I Help You SM ” spoken dialog system. We show that augmenting standard lexical and prosodic features with contextual features that exploit the structure of spoken dialog and track user stateincreases classification accuracy by 2.6%.
An Examination of the Factor Structure of the Hartford Geriatric Social Work Competency Scale-II Assessment and Intervention Subscales

The Hartford Geriatric Social Work Competency Scale II (GSWCS-II) is the gold standard for assessment of social work competency and confidence in gerontological practice behaviors, but minimal research to date has examined the factor structure of this important instrument. This study employed exploratory factor analysis to determine the underlying structure of the measure's subscales related to assessment and intervention. Results indicate that the Assessment and Intervention subscales each have two underlying components that differentiate between core geriatric competencies necessary for all social workers and competencies required for specialists in aging. Findings have implications for the evaluation of competency and self-efficacy in gerontological social work, furthering the application of the GSWCS-II for both social work education and practice settings.
The state of the psychological contract and employees’ intention to leave: The mediating role of employee well-being

Changes in the work environment can influence employees’ perception of the employment relationship, their psychological contracts, and consequently, their work-related attitudes and behaviour. The study aimed to demonstrate the mechanism through which the state of employees’ psychological contract impacts employees’ intention to leave employment and the role of employee well-being as a mediator. Cross-sectional data were obtained from employees representing various organisations (N = 246). Structural equation modelling demonstrated that employee well-being mediates the relationship between the state of the psychological contract and an employee’s intention to leave the organisation. The study stresses the importance of designing individual-level interventions to enhance well-being in the workplace, which should form part of the retention strategy of any organisation.
Interaction of Hepatitis C Virus-Like Particles and Cells: a Model System for Studying Viral Binding and Entry

ABSTRACT Hepatitis C virus-like particles (HCV-LPs) containing the structural proteins of HCV H77 strain (1a genotype) was used as a model for HCV virion to study virus-cell interaction. HCV-LPs showed a buoyant density of 1.17 to 1.22 g/cm3 in a sucrose gradient and formed double-shelled particles 35 to 49 nm in diameter. Flow cytometry analysis by an indirect method (detection with anti-E2 antibody) and a direct method (use of dye-labeled HCV-LPs) showed that HCV-LPs binds to several human hepatic (primary hepatocytes, HepG2, HuH7, and NKNT-3) and T-cell (Molt-4) lines. HCV-LPs binding to cells occurred in a dose- and calcium-dependent manner and was not mediated by CD81. Scatchard plot analysis suggests the presence of two binding sites for HCV-LPs with high (Kd ∼1 μg/ml) and low (Kd ∼50 to 60 μg/ml) affinities of binding. Anti-E1 and -E2 antibodies inhibited HCV-LPs binding to cells. While preincubation of HCV-LPs with very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), or high-density lipoprotein (HDL) blocked its binding to cells, preincubation of cells with VLDL, LDL, HDL, or anti-LDL-R antibody did not. Confocal microscopy analysis showed that, after binding to cells, dye-labeled HCV-LPs were internalized into the cytoplasm. This process could be inhibited with anti-E1 or anti-E2 antibodies, suggesting that E1 and E2 proteins mediate HCV-LPs binding and, subsequently, their entry into cells. Altogether, our results indicate that HCV-LPs can be used to further characterize the mechanisms involved in the early steps of HCV infection.
Rethinking the association between extraversion and job satisfaction: The role of interpersonal job context

The person-job fit literature indicates that job satisfaction is contingent on the congruence between the individual and the job. We propose that interpersonal job context, the extent to which incumbents are exposed to social interaction on the job, magnifies the relationship between extraversion and job satisfaction. Using two large archival data sets ( Ns = 5,849; 23,376) with job-level information from O* NET, we demonstrated (1) a main effect of job-level extraversion on interpersonal job context, and (2) a cross-level moderating effect of interpersonal job context on the individual-level extraversion-satisfaction relationship. These results suggest that extraverts are more likely to hold and be satisfied in jobs rich in social interaction. This research emphasizes the need for researchers and practitioners to consider personality-job context congruence. Practitioner points We examined the relationship between personality and job satisfaction in two large archival data sets. , At the job level, extraverts were more likely than introverts to occupy jobs requiring spontaneous interpersonal contact. , Interpersonal job context magnified the association between extraversion and job satisfaction. , It is important to consider the fit between personality and the job context, beyond personality's main effects.
Early stage radiation‐induced lung injury detected using hyperpolarized 129Xe Morphometry: Proof‐of‐concept demonstration in a rat model

Radiation‐induced lung injury (RILI) is still the major dose‐limiting toxicity related to lung cancer radiation therapy, and it is difficult to predict and detect patients who are at early risk of severe pneumonitis and fibrosis. The goal of this proof‐of‐concept preclinical demonstration was to investigate the potential of hyperpolarized 129Xe diffusion‐weighted MRI to detect the lung morphological changes associated with early stage RILI.
Lipoprotein-Dependent and -Independent Immune Responses to Spirochetal Infection

ABSTRACT In this study, we used the epidermal suction blister technique, in conjunction with multiparameter flow cytometry, to analyze the cellular and cytokine responses elicited by intradermal injection of human volunteers with synthetic analogs for spirochetal lipoproteins and compared the responses to findings previously reported from patients with erythema migrans (EM). Compared with peripheral blood (PB), lipopeptides derived from the N termini of the Borrelia burgdorferi outer surface protein C and the 17-kDa lipoprotein of Treponema pallidum (OspC-L and 17-L, respectively) elicited infiltrates enriched in monocytes/macrophages and dendritic cells (DCs) but also containing substantial percentages of neutrophils and T cells. Monocytoid (CD11c+) and plasmacytoid (CD11c−) DCs were selectively recruited to the skin in ratios similar to those in PB, but only the former expressed the activation/maturation surface markers CD80, CD83, and DC-SIGN. Monocytes/macrophages and monocytoid DCs, but not plasmacytoid DCs, displayed significant increases in surface expression of Toll-like receptor 1 (TLR1), TLR2, and TLR4. Staining for CD45RO and CD27 revealed that lipopeptides preferentially recruited antigen-experienced T-cell subsets; despite their lack of antigenicity, these agonists induced marked T-cell activation, as evidenced by surface expression of CD69, CD25, and CD71. Lipopeptides also induced significant increases in interleukin 12 (IL-12), IL-10, gamma interferon, and most notably IL-6 without corresponding increases in serum levels of these cytokines. Although lipopeptides and EM lesional infiltrates shared many similarities, differences were noted in a number of immunologic parameters. These studies have provided in situ evidence for a prominent “lipoprotein effect” during human infection while at the same time helping to pinpoint aspects of the cutaneous response that are uniquely driven by spirochetal pathogens.
The link between Parkinson's disease and breast and prostate cancers: A meta-analysis

Purpose: Clinical observations have shown an increased morbidity for breast cancer or prostate cancer in patients with Parkinson's disease (PD), however, other reports have noted contradictory results. This pooled analysis was utilized to test whether PD is associated with the risk of breast or prostate cancer. Methods: We searched PubMed, Embase and Cochrane library and conducted a meta-analysis to clarify the correlation of PD with breast and/or prostate cancer risk. We identified 16 eligible articles from which odds ratios with 95% confident intervals were assessed as main measures in the pooled estimation. Subgroup analyses and cumulative meta-analysis were also performed. Results: Our results showed no PD risk associated with breast or prostate cancer in the overall population, which was supported by the results of cumulative meta-analyses. The subgroup analyses suggested no significant risk of breast or prostate cancer in patients with PD within relevant subsets, i.e. gender, ethnicity, PD diagnosis time or study design. No evidence of publication bias was observed across the involved studies. Conclusions: This meta-analysis indicates a lack of association between PD and risk of breast or prostate cancer.
Novel role of the nociceptin system as a regulator of glutamate transporter expression in developing astrocytes

Our previous results showed that oligodendrocyte development is regulated by both nociceptin and its G‐protein coupled receptor, the nociceptin/orphanin FQ receptor (NOR). The present in vitro and in vivo findings show that nociceptin plays a crucial conserved role regulating the levels of the glutamate/aspartate transporter GLAST/EAAT1 in both human and rodent brain astrocytes. This nociceptin‐mediated response takes place during a critical developmental window that coincides with the early stages of astrocyte maturation. GLAST/EAAT1 upregulation by nociceptin is mediated by NOR and the downstream participation of a complex signaling cascade that involves the interaction of several kinase systems, including PI‐3K/AKT, mTOR, and JAK. Because GLAST is the main glutamate transporter during brain maturation, these novel findings suggest that nociceptin plays a crucial role in regulating the function of early astrocytes and their capacity to support glutamate homeostasis in the developing brain.
Psychosocial effects of community noise: cross sectional study of school children in urban center of Skopje, Macedonia.

AIM To assess noise exposure in school children in urban center in different residential areas and to examine psychosocial effects of chronic noise exposure in school children, taking into account their socioeconomic status. METHODS We measured community noise on specific measurement points in residential-administrative-market area and suburban residential area. We determined the average energy-equivalent sound level for 8 hours (LAeq, 8 h) or 16 hours (LAeq, 16 h) and compared measured noise levels with World Health Organization (WHO) guidelines. Psychological effects were examined in two groups of children: children exposed to noise level LAeq, 8 h >55 dBA (n=266) and children exposed to noise level LAeq, 8 h <55 dBA (n=263). The examinees were schoolchildren of 10-11 years of age. We used a self-reported questionnaire for each child - Anxiety test (General Anxiety Scale) and Attention Deficit Disorder Questionnaire intended for teachers to rate children's behavior. We used Mann Whitney U test and multiple regression for identifying the significance of differences between the two study groups. RESULTS School children who lived and studied in the residential-administrative-market area were exposed to noise levels above WHO guidelines (55 dBA), and school children who lived and studied in the suburban residential area were exposed to noise levels below WHO guidelines. Children exposed to LAeq, 8 h >55 dBA had significantly decreased attention (Z=-2.16; p=0.031), decreased social adaptability (Z =-2.16; p=0.029), and increased opposing behavior in their relations to other people (Z=-3; p=0.001). We did not find any correlation between socioeconomic characteristics and development of psychosocial effects. CONCLUSION School children exposed to elevated noise level had significantly decreased attention, and social adaptability, and increased opposing behavior in comparison with school children who were not exposed to elevated noise levels. Chronic noise exposure is associated with psychosocial effects in school children and should be taken as an important factor in assessing the psychological welfare of the children.
Trends in the Prevalence of Depression in Hospitalized Patients with Type 2 Diabetes in Spain: Analysis of Hospital Discharge Data from 2001 to 2011

Background This study aims to describe trends in the prevalence of depression among hospitalized patients with type 2 diabetes in Spain, 2001–2011. Methods We selected patients with a discharge diagnosis of type 2 diabetes using national hospital discharge data. Discharges were grouped by depression status. Prevalence of depression globally and according to primary diagnoses based on the Charlson comorbidity index (CCI) were analyzed. We calculated length of stay (LOHS) and in-hospital mortality (IHM). Multivariate analysis was adjusted by age, year and comorbidity. Results From 2001 to 2011, 4,723,338 discharges with type 2 diabetes were identified (4.93% with depression). Prevalence of depression in diabetic patients increased from 3.54% in 2001 to 5.80% in 2011 (p<0.05). The prevalence of depression was significantly higher in women than in men in each year studied and increased from 5.22% in 2001 to 9.24% in 2011 (p<0.01). The highest prevalence was observed in the youngest age group (35–59 years). The median LOHS decreased significantly over this period. Men with diabetes and depression had higher IHM than women in all the years studied (p<0.05). Older age and greater comorbidity were significantly associated with a higher risk of dying, among diabetic patients with concomitant depression. Conclusions Prevalence of depression increased significantly among hospitalized diabetic patients from 2001 to 2011 even if the health profile and LOHS have improved over this period. Programs targeted at preventing depression among persons with diabetes should be reinforced in Spain.
Violence Abuse: A Commentary on Donald G. Dutton's Rethinking Domestic Understanding the Complexities of Feminist Perspectives on Woman

can be found at: Violence Against Women Additional services and information for http://vaw.sagepub. com/cgi/alerts Email Alerts: http://vaw.sagepub. com/subscriptions Subscriptions: http://www.sagepub. com/journalsReprints.nav Reprints: http://www.sagepub. com/journalsPermissions.nav Permissions: http://vaw.sagepub. com/cgi/content/refs/13/8/874 SAGE Journals Online and HighWire Press platforms): (this article cites 17 articles hosted on the Citations
Novel Soft Switching Inverter for Brushless DC Motor using Fuzzy Logic

Brushless DC motor (BLDCM) has been broadly used in drive systems and servo control because of its fast response, high power density, high efficiency, low inertia, high reliability and maintenance free. It is however driven by a hard-switching PWM inverter, which has low switching frequency, high switching loss, high electro-magnetic interference (EMI), high acoustic noise and low efficiency, etc. To solve these problems of the hard switching inverter, many soft switching inverters have been designed in the past. Unfortunately, high device voltage stress, large dc link voltage ripples, complex control scheme and so on are noticed in the soft switching inverters. This paper introduces a novel fuzzy logic based soft-switching inverter using transformer, which can generate dc link voltage notches during chopping which minimize the drawbacks of soft switching. Hence all switches work in zero voltage switching condition. The operation principle and control scheme of the inverter are analyzed and performance of the fuzzy controller is compared with conventional PI controller. The simulation results show that the fuzzy controller renders a better transient response than the PI controller resulting in negligible overshoot and smaller settling time. Simulation results are proposed to verify the theoretical analysis.
Spectroscopic evidence of coexistence of clusters based on low (α) and high temperature (β) GeS2 crystalline phases in glassy germanium disulfide matrix

Technologically modified g-GeS<sub>2</sub>(TiVj) glasses prepared by melt quenching from different temperatures (T<sub>i</sub>) and with different cooling rates (Vj) were studied using Raman spectroscopy and model calculations. Differential Raman spectra {I<sub>R</sub>GeS<sub>2</sub>(T<sub>i</sub>V<sub>1</sub>)-I<sub>R</sub>GeS2(T<sub>2</sub>V<sub>1</sub>)} showed the splitting of the main, most intensive wide-band, centered at 340 cm<sup>-1</sup>. The position of the peaks found in the differential Raman spectra near 340 and 360 cm<sup>-1</sup> and position of the main vibrational modes in the ordinary Raman spectra of low- and high-temperature crystalline phases (α-, β-GeS2) were found to be in good agreement. Vibrational spectra of 4- and 6-member ring fragments selected from crystalline structure of β-GeS<sub>2</sub> were also determined using model calculations. Based on rings composed of GeS<sub>4</sub> tetrahedrons connected by corners and edges the structural interpretation of the vibrational bands of g-GeS<sub>2</sub>(TiVj) at 360, 370 and 433 cm<sup>-1</sup> in their Raman spectra was performed. The existence of homopolar Ge-Ge and S-S “defect” bonds in the structure of germanium disulfide glasses was proved by formation of 5-member rings. Formation of fragments of both low- and high-temperature α-, β-GeS<sub>2</sub> crystalline phases in the structure of g-GeS<sub>2</sub>(TiVj) glasses indicates the existence of mixed medium-range ordering in g-GeS<sub>2</sub>.
Making Use of Existing Lexical Resources to Build a Verbnet like Classification of French Verbs. (Acquisition de classes verbales pour le français)

Classifications which group together verbs and a set of shared syntactic and semantic properties have proven useful both in linguistics and in Natural Language Processing tasks. However, for French this type of classifications is not available in a format suitable for automated processing. In addition, most existing approaches for automatically acquiring verb classes fail to associate the verb classes produced with an explicit characterisation of the syntactic and semantic properties shared by the class members. Here we propose a novel approach to verb clustering which addresses these shortcomings. We classify French verbs using two clustering methods, a symbolic method called Formal Concept Analysis (FCA) and a probabilistic neural clustering method called Incremental Growing Neural Gas with Feature Maximisation (IGNGF). The obtained classes group together verbs, subcategorisation frames and thematic grids. We apply this approach to French data consisting of roughly 4000 verbs and 350 subcategorisation frames, and evaluate both the clusters obtained (i.e., verb classes) and the features labeling each cluster (i.e., syntactic frames and thematic grids). The results suggest that both classification methods can be used to bootstrap a Verbnet style classification for French such that the verb classes it contains (i) are reasonably clean and (ii) associate verbs with partial information about subcategorisation frames and thematic grids. The obtained classifications are complementary. While the FCA classification better represents verb polysemy (better F-measure and recall compared to reference data) the IGNGF classification performed better with respect to the produced verb classes and when used in a task based evaluation.