Miracle-dz/UniFAR-FTUs huggingface dataset:
Diminished GATA4 Protein Levels Contribute to Hyperglycemia-induced Cardiomyocyte Injury*

Hyperglycemia is an independent risk factor for diabetic heart failure. However, the mechanisms that mediate hyperglycemia-induced cardiac damage remain poorly understood. The transcription factor GATA4 is essential for cardiac homeostasis, and its protein levels are dramatically reduced in the heart in response to diverse pathologic stresses. In this study, we investigated if hyperglycemia affects GATA4 expression in cardiomyocytes and if enhancing GATA4 signaling could attenuate hyperglycemia-induced cardiomyocyte injury. In cultured rat cardiomyocytes, high glucose (HG, 25 or 40 mm) markedly reduced GATA4 protein levels as compared with normal glucose (NG, 5.5 mm). Equal amount of mannitol did not affect GATA4 protein expression (NG, 100 ± 12%; mannitol, 97 ± 8%, versus HG, 43 ± 16%, p < 0.05). The GATA4 mRNA content, either steady-state or polysome-associated, remained unchanged. HG-induced GATA4 reduction was reversed by MG262, a specific proteasome inhibitor. HG did not activate the ubiquitin proteasome system (UPS) in cardiomyocytes as indicated by a UPS reporter, nor did it increase the peptidase activities or protein expression of the proteasomal subunits. However, the mRNA levels of ubiquitin-protein isopeptide ligase (E3) carboxyl terminus of Hsp70-interacting protein (CHIP) were markedly increased in HG-treated cardiomyocytes. CHIP overexpression promoted GATA4 protein degradation, whereas small interfering RNA-mediated CHIP knockdown prevented HG-induced GATA4 depletion. Moreover, overexpression of GATA4 blocked HG-induced cardiomyocyte death. Also, GATA4 protein levels were diminished in the hearts of streptozotocin and db/db diabetic mice (44 ± 7% and 67 ± 13% of control, p < 0.05), which correlated with increased CHIP mRNA abundance. In summary, increased GATA4 protein degradation may be an important mechanism that contributes to hyperglycemic cardiotoxicity.
Determination of platelet‐bound glycoprotein‐specific autoantibodies by flow cytometric immunobead assay in primary immune thrombocytopenia

Objectives: Primary immune thrombocytopenia (ITP) is an autoimmune disorder characterized by premature platelet destruction induced by autoantibodies directed against platelet glycoproteins (GPs). Despite being a clinically important disorder, ITP lacks a feasible diagnostic assay for routine clinical use. This study was meant to evaluate a newly developed flow cytometric immunobead assay for determination of platelet‐bound GP‐specific autoantibodies in comparison with indirect monoclonal antibody‐specific immobilization of platelet antigen (MAIPA) in the diagnosis of ITP.
Adaptive model-based visual stabilization of image sequences using feedback

Visual stabilization proposed in this paper compensates changes of the scene caused by motion and deformation of an observed object. This is of high importance in computer-assisted beating heart surgery, where the views of the beating heart should be stabilized. The proposed model-based method defines visual stabilization as a transformation of the current image sequence to a stabilized image sequence. This transformation incorporates physical model of the observed object and model of the measurement process. In contrast to standard approaches, the quality of the visual stabilization is continuously evaluated and improved in two aspects. On the one hand, discretization errors are reduced. On the other hand, the parameters of the underlying models are adjusted. The performance of the proposed method is evaluated in an experiment with a pressure-regulated artificial heart. Compared with standard methods, the model-based method provides higher accuracy, which is additionally improved by a feedback mechanism.
SelfSync: a dynamic round-trip engineering environment

Model-Driven Engineering (MDE) advocates the generation of software applications from models, which are views on certain aspects of the software. In this paper, we focus on a particular setup which consists of a graphical data modeling view and a view on an object-oriented implementation, which can be either textual or graphical. A challenge that arizes in the context of MDE is the notion of Round-Trip Engineering (RTE), where elements from both views can be manipulated and thus need to be synchronized. We systematically identify four fundamental RTE scenarios. In this paper, we employ the framework of these scenarios for explaining SelfSync, our approach and tool for providing dynamic support for RTE. In SelfSync, the entities of the data modeling view and the corresponding implementation objects are one and the same. Additionally, we present a comparison with related work accompanied by an extensive discussion.
Bronchoalveolar lavage findings suggest two different forms of childhood asthma

Background It seems plausible that children with atopy and persistent asthma symptoms will, like their adult counterparts, have chronic airways inflammation. However, many young children with no other atopic features have episodic wheezing that is triggered solely by viral respiratory infections. Little is known as to whether airways inflammation occurs in these two asthma patterns during relatively asymptomatic periods.
Are ecosystem services stabilized by differences among species? A test using crop pollination

Biological diversity could enhance ecosystem service provision by increasing the mean level of services provided, and/or by providing more consistent (stable) services over space and time. Ecological theory predicts that when an ecosystem service is provided by many species, it will be stabilized against disturbance by a variety of ‘stabilizing mechanisms. ’ However, few studies have investigated whether stabilizing mechanisms occur in real landscapes affected by human disturbance. We used two datasets on crop pollination by wild native bees to screen for and differentiate among three stabilizing mechanisms: density compensation (negative co-variance among species' abundances); response diversity (differential response to environmental variables among species); and cross-scale resilience (response to the same environmental variable at different scales by different species). In both datasets, we found response diversity and cross-scale resilience, but not density compensation. We conclude that stabilizing mechanisms may contribute to the stability of pollination services in our study areas, emphasizing the insurance value of seemingly ‘redundant’ species. Furthermore, the absence of density compensation that we found at the landscape scale contrasts with findings of previous small-scale experimental and modelling work, suggesting that we should not assume that density compensation will stabilize ecosystem services in real landscapes.
Anxious or Angry? Effects of Discrete Emotions on the Perceived Helpfulness of Online Reviews

This paper explores the effects of emotions embedded in a seller review on its perceived helpfulness to readers. Drawing on frameworks in literature on emotion and cognitive processing, we propose that over and above a well-known negativity bias, the impact of discrete emotions in a review will vary, and that one source of this variance is reader perceptions of reviewers' cognitive effort. We focus on the roles of two distinct, negative emotions common to seller reviews: anxiety and anger. In the first two studies, experimental methods were utilized to identify and explain the differential impact of anxiety and anger in terms of perceived reviewer effort. In the third study, seller reviews from Yahoo! Shopping web sites were collected to examine the relationship between emotional review content and helpfulness ratings. Our findings demonstrate the importance of examining discrete emotions in online word-of-mouth, and they carry important practical implications for consumers and online retailers.
Antioxidative and Antiapoptotic Effects of Delta-Opioid Peptide [D-Ala2, D-Leu5] Enkephalin on Spinal Cord Ischemia-Reperfusion Injury in Rabbits

Background: In our previous study, we found that regional administration of delta-opioid peptide [D-Ala2, D-Leu5] enkephalin (DADLE) could provide dose-dependent protection on spinal cord ischemia-reperfusion (I/R) injury in rabbits. However, the relative protective molecular mechanisms underlying this neuroprotection remain unclear. The purpose of this study was to investigate whether DADLE provided the protection in spinal cord I/R injury through its antioxidant property by decreasing malondialdehyde (MDA) and nitric oxide (NO) levels and increasing glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels and through its antiapoptotic capacity by inhibiting caspase-3 and p53 expression. Methods: The rabbits were divided into three groups. The animals in Group NS and Group DADLE were administered with normal saline (NS) or DADLE via aorta during 30 min of ischemia respectively, while the one in Group Sham received no intervention. During the period of reperfusion, the rabbit's blood samples were collected for enzyme-linked immunoabsorbent assay (ELISA) examinations of MDA, NO, GSH-Px and SOD. At 48 h after reperfusion, the lumbar spinal cords were harvested for immunohistochemical, real-time polymerase chain reaction (PCR) and western blot studies to detect the caspase-3 and p53 expressions. Results: The activities of serum MDA and NO showed significant reductions in the DADLE group as compared with the control group. By contrast, the levels of serum GSH-Px and SOD were significantly higher in the DADLE group than those in the NS group. In addition, caspase-3 and p53 expression were significantly increased in the NS group, while DADLE mitigated these changes. Conclusions: The protective effects of DADLE at the dosage of 0.05 mg/kg may be related to its antioxidant and antiapoptosis properties in the rabbit model of spinal cord I/R injury.
No cure for the common cold

Background: A study was undertaken to assess the function of the hypothalamic-pituitary-adrenal axis (HPA) in a group of asthmatic children before and after treatment with inhaled corticosteroids. Methods: Thirty prepubertal patients of mean (SD) age 6.7 (1.8) years were treated with inhaled corticosteroids. All children underwent a corticotrophin releasing hormone (CRH) test with evaluation of serum cortisol and adrenocorticotrophin hormone (ACTH) levels before and after 3 months of treatment. Twenty four hour urine samples were also collected to measure free cortisol (UFC) excretion. Results: Subjects showed no difference between basal serum cortisol levels (mean change –18; 95% CI –41 to 5; p=0.118) and delta (peak minus basal) levels (mean change –13; 95% CI –38 to 12; p=0.308) before and after treatment, whereas the peak cortisol level (mean change –31; 95% CI –55 to –7; p=0.013) and area under the curve (AUC) (mean change –175; 95% CI –288 to –63; p=0.003) after CRH were significantly lower following treatment. Basal, peak and AUC ACTH were significantly lower after treatment (p<0.05, p=0.004 and p=0.003, respectively), while delta ACTH was similar before and after treatment ((mean change –12; 95% CI – 31 to –7; p=0.199). No significant reduction in 24 hour UFC was observed after the treatment period (before 14.9 (7.1), after 15.0 (11.6); mean change 0.1, 95% CI –5.2 to 5.4; p=0.967). No correlation was found between UFC and any of the parameters of cortisol excretion following the CRH test, either before or after treatment. Conclusions: These data suggest that, at the dosage and for the treatment period used, inhaled steroids do not seem to suppress the HPA axis in the majority of patients. The CRH test may be more sensitive than 24 hour UFC and morning plasma cortisol levels in evaluating systemic activity of inhaled corticosteroid treatment.
L1-error estimates for numerical approximations of Hamilton-Jacobi-Bellman equations in dimension 1

The goal of this paper is to study some numerical approximations of particular Hamilton-Jacobi-Bellman equations in dimension 1 and with possibly discontinuous initial data. We investigate two anti-diffusive numerical schemes, the first one is based on the Ultra-Bee scheme and the second one is based on the Fast Marching Method. We prove the convergence and derive $L^1$-error estimates for both schemes. We also provide numerical examples to validate their accuracy in solving smooth and discontinuous solutions.
Development of advanced parking assistance system

The development of advanced parking assistance system for assisting the driver to accomplish the parking task is described in this paper. The main issues in implementing the assistance system are the position sensing and the human guidance through human interface. The sensor suite selection, filtering scheme, guidance scheme, human interface scheme development process are described. The developed system prototype and experimental results are also presented.
The Soluble Form of Toll-Like Receptor 2 Is Elevated in Serum of Multiple Sclerosis Patients: A Novel Potential Disease Biomarker

Multiple sclerosis (MS) is an immune-mediated inflammatory demyelinating disease of the central nervous system. It was previously shown that toll-like receptor (TLR)-2 signaling plays a key role in the murine experimental autoimmune encephalomyelitis (EAE) model of MS, and that TLR2-stimulation of regulatory T cells (Tregs) promotes their conversion to T helper 17 (Th17) cells. Here, we sought potential sources of TLR2 stimulation and evidence of TLR2 activity in MS patient clinical samples. Soluble TLR2 (sTLR2) was found to be significantly elevated in sera of MS patients (n = 21), in both relapse and remission, compared to healthy controls (HC) (n = 24). This was not associated with the acute phase reaction (APR) as measured by serum C-reactive protein (CRP) level, which was similarly increased in MS patients compared to controls. An independent validation cohort from a different ethnic background showed a similar upward trend in mean sTLR2 values in relapsing-remitting MS (RRMS) patients, and significant differences in sTLR2 values between patients and HC were preserved when the data from the two cohorts were pooled together (n = 41 RRMS and 44 HC, P = 0.0006). TLR2-stimulants, measured using a human embryonic kidney (HEK)-293 cells transfectant reporter assay, were significantly higher in urine of MS patients than HC. A screen of several common urinary tract infections (UTI)-related organisms showed strong induction of TLR2-signaling in the same assay. Taken together, these results indicate that two different markers of TLR2-activity—urinary TLR2-stimulants and serum sTLR2 levels—are significantly elevated in MS patients compared to HC.
Intracluster Moves for Constrained Discrete-Space MCMC

This paper addresses the problem of sampling from binary distributions with constraints. In particular, it proposes an MCMC method to draw samples from a distribution of the set of all states at a specified distance from some reference state. For example, when the reference state is the vector of zeros, the algorithm can draw samples from a binary distribution with a constraint on the number of active variables, say the number of 1's. We motivate the need for this algorithm with examples from statistical physics and probabilistic inference. Unlike previous algorithms proposed to sample from binary distributions with these constraints, the new algorithm allows for large moves in state space and tends to propose them such that they are energetically favourable. The algorithm is demonstrated on three Boltzmann machines of varying difficulty: A ferromagnetic Ising model (with positive potentials), a restricted Boltzmann machine with learned Gabor-like filters as potentials, and a challenging three-dimensional spin-glass (with positive and negative potentials).
Models and Concepts for Socio-Technical Complex Systems: Towards Fractal Social Organizations

We introduce fractal social organizations—a novel class of socio-technical complex systems characterized by a distributed, bio-inspired, hierarchical architecture. Based on a same building block that is recursively applied at different layers, said systems provide a homogeneous way to model collective behaviors of different complexity and scale. Key concepts and principles are enunciated by means of a case study and a simple formalism. As preliminary evidence of the adequacy of the assumptions underlying our systems here, we define and study an algebraic model for a simple class of social organizations. We show how despite its generic formulation, geometric representations of said model exhibit the spontaneous emergence of complex hierarchical and modular patterns characterized by structured addition of complexity and fractal nature—which closely correspond to the distinctive architectural traits of our fractal social organizations. Some reflections on the significance of these results and a view to the next steps of our research conclude this contribution. Copyright © 2013 John Wiley & Sons, Ltd.
Resident perceptions of urban stream restoration and water quality in South Korea

This research explores if individual preferences for the major functions of stream restoration processes correlate water quality variations in an urban watershed of South Korea. We use a set of results from an analytical hierarchy process model to rank the major stream restoration functions and compare citizens' preferences for “water quality” improvement during stream restoration based on the water quality index (WQI) and ordinary least square regression. This study identifies the correlation between the WQI and individual perceptions regarding stream restoration. Regional context and individual characteristics also influence the demands and awareness of citizens pertaining to stream restoration. Our findings highlight the importance of understanding residents' perceptions and demographic information, including education, marriage status, gender, and residence period in different subwatersheds. The largest change (28.29) in the WQI score of one subwatershed was correlated with the participants' perception of water quality changes and water pollution (analytical hierarchy process index: 66.8%). On the basis of the survey results, we recommend that a human–ecological interdependent and adaptive consultative body is established in each subwatershed to successfully implement stream restoration projects.
Solute dispersion in a variably saturated sand

The understanding of solute dispersion in unsaturated porous media is still nascent. This work focuses on the measurement of solute dispersion in porous media at various levels of pore‐volume saturation and on the parameterization of longitudinal dispersivity in terms of the saturation ratio and a soil's textural characteristics. The dispersion coefficient was determined in partly saturated columns packed with Toyoura sand or glass beads under steady state flow. A 0.0282 N sodium chloride solution was used as a nonreactive tracer in a series of laboratory experiments in which, for a fixed water content, the average pore‐water velocity was varied. The column experiments showed that the dispersion coefficient increases linearly with increasing water velocity for a fixed water content and that the slope of the dispersion coefficient versus water velocity relationship diminishes as the water content is increased. The Peclet number (Pe) was fitted to the experimental tracer data by a nonlinear function Pe = aSrb, in which Sr is the saturation ratio and a and b are textured‐dependent parameters. The dispersivity (α, in centimeters) of the test columns' porous media was then estimated in terms of the saturation ratio and the median grain size (d50, in centimeters) by the power law α = d50/Pe = d50/aSrb, which provides a specific functional relationship among a porous medium's dispersion properties, the saturation ratio, and the medium's textural characteristics.
Prevalence and Characteristics of Fetal Alcohol Spectrum Disorders

OBJECTIVES: To determine the prevalence and characteristics of fetal alcohol spectrum disorders (FASD) among first grade students (6- to 7-year-olds) in a representative Midwestern US community. METHODS: From a consented sample of 70.5% of all first graders enrolled in public and private schools, an oversample of small children (≤25th percentile on height, weight, and head circumference) and randomly selected control candidates were examined for physical growth, development, dysmorphology, cognition, and behavior. The children’s mothers were interviewed for maternal risk. RESULTS: Total dysmorphology scores differentiate significantly fetal alcohol syndrome (FAS) and partial FAS (PFAS) from one another and from unexposed controls. Alcohol-related neurodevelopmental disorder (ARND) is not as clearly differentiated from controls. Children who had FASD performed, on average, significantly worse on 7 cognitive and behavioral tests and measures. The most predictive maternal risk variables in this community are late recognition of pregnancy, quantity of alcoholic drinks consumed 3 months before pregnancy, and quantity of drinking reported for the index child’s father. From the final multidisciplinary case findings, 3 techniques were used to estimate prevalence. FAS in this community likely ranges from 6 to 9 per 1000 children (midpoint, 7.5), PFAS from 11 to 17 per 1000 children (midpoint, 14), and the total rate of FASD is estimated at 24 to 48 per 1000 children, or 2.4% to 4.8% (midpoint, 3.6%). CONCLUSIONS: Children who have FASD are more prevalent among first graders in this Midwestern city than predicted by previous, popular estimates.
Community Design and Access to Recreational Facilities as Correlates of Adolescent Physical Activity and Body-Mass Index.

BACKGROUND Neighborhood-level environmental features have been associated with adult physical activity and weight status, but this link has not been established for adolescents. METHODS Community design and access to recreational facilities variables were derived using geographic information systems (GIS) for 799 adolescents (age 11 to 15 y, mean = 12.8 y, 53% girls, 43% ethnic minority). Environment variables were calculated for a 1-mile buffer around each participant's residence. Accelerometers measured min/d of physical activity. RESULTS Number of nearby recreation facilities and number of nearby parks correlated positively with girls' physical activity, and intersection density inversely related to girls' physical activity. Retail floor area ratio correlated positively with boys' physical activity. No community design or access to recreation variables were related to BMI-percentile. CONCLUSIONS There was limited evidence that both community design and access to recreation facilities variables were associated with adolescent physical activity, but additional built environment variables need to be studied that have particular relevance for youth.