Miracle-dz/UniFAR-FTUs huggingface dataset:
From 9-1-1 call to death: Evaluating traumatic deaths in seven regions for early recognition of high-risk patients

BACKGROUND This study aimed to characterize initial clinical presentations of patients served by emergency medical services (EMS) who die following injury, with particular attention to patients with occult (“talk-and-die”) presentations. METHODS This was a population-based, multiregion, mixed-methods retrospective cohort study of fatally injured children and adults evaluated by 94 EMS agencies transporting to 122 hospitals in seven Western US regions from 2006 to 2008. Fatalities were divided into two main groups: occult injuries (talk-and-die; Glasgow Coma Scale [GCS] score ≥ 13, no cardiopulmonary arrest, and no intubation) versus overt injuries (all other patients). These groups were further subdivided by timing of death: early (<48 hours) versus late (>48 hours). We then compared demographic, physiologic, procedural, and injury patterns using descriptive statistics. We also used qualitative methods to analyze available EMS chart narratives for contextual information from the out-of-hospital encounter. RESULTS During the 3-year study period, 3,358 persons served by 9-1-1 EMS providers died, with 1,225 (37.1%) in the field, 1,016 (30.8%) early in the hospital, and 1,060 (32.1%) late in the hospital. Of the 2,133 patients transported to a hospital, there were 612 (28.7%) talk-and-die patients, of whom 114 (18.6%) died early. Talk-and-die patients were older (median age, 81 years; interquartile range, 67–87 years), normotensive (median systolic blood pressure, 138 mm Hg; interquartile range, 116–160 mm Hg), commonly injured by falls (71.3%), and frequently (52.4%) died in nontrauma hospitals. Compared with overtly injured patients, talk-and-die patients had relatively fewer serious head injuries (13.7%) but more frequent extremity injuries (20.3% vs. 10.6%) and orthopedic interventions (25.3% vs. 5.0%). EMS personnel often found talk-and-die patients lying on the ground with hip pain or extremity injuries. CONCLUSION Patients served by EMS who “talk-and-die” are typically older adults with falls, transported to nontrauma hospitals, with subtle clinical indications of the severity of their injuries. Improving recognition of talk-and-die patients may avoid fatal outcomes in a portion of these patients. LEVEL OF EVIDENCE Epidemiologic study, level III.
SUXES - user experience evaluation method for spoken and multimodal interaction

Much work remains to be done with subjective evaluations of speech-based and multimodal systems. In particular, user experience is still hard to evaluate. SUXES is an evaluation method for collecting subjective metrics with user experiments. It captures both user expectations and user experiences, making it possible to analyze the state of the application and its interaction methods, and compare results. We present the SUXES method with examples of user experiments with different applications and modalities. Index Terms: evaluation, subjective metrics, user experience
Quickflow response to forest harvesting and recovery in a northern hardwood forest landscape

Forest harvesting often increases catchment quickflow (QF, water delivered rapidly to the stream channel), a metric of high‐flow events controlling a catchment's solute and sediment export. Nevertheless, our understanding of QF responses to various silvicultural strategies (e.g., clearcutting, selection harvest, and shelterwood harvest) is incomplete. We present a 31‐year examination of QF delivery from treatment (clearcut, selection harvest, and shelterwood harvest) and control catchments in a deciduous forest landscape in central Ontario, Canada. Growing season root‐zone storage capacity was estimated using a water balance approach to evaluate temporal changes in QF response to precipitation (P) for pretreatment and posttreatment periods. Threshold relationships between QF and P were assessed for control and treatment catchments for pretreatment and posttreatment periods using piecewise regression. Root‐zone storage capacity demarcated shifts in the hydrologic regime arising from forest harvesting and subsequent regeneration. This was particularly pronounced for clearcutting where postharvest decline in root‐zone storage capacity was followed by a rise to preharvest values. Similar pretreatment threshold relationships between QF and P, and near‐identical P thresholds for producing significant QF, reflected similar soil and overburden depths in the catchments. Harvesting effects were indicated by increases in QF/P ratios for relative small P and the number of P events that generated QF, thus changing treatment QF vs. P threshold relationships. Prior to harvesting there was no significant increase in QF with P below a threshold P of 35–45 mm; however, there was a significant QF vs. P relationship below this threshold for all treatments postharvest. Clearcutting increased the number of QF events for the entire postharvest period and the first 9‐year postharvest compared to the other treatments; nevertheless, evidence for intertreatment differences in total QF depth delivered from the catchments during the growing season was inconclusive. Our work suggests that changes in threshold relationships between QF and P, coupled with knowledge of the physical processes underlying them, are useful when evaluating hydrologic responses to forest harvesting.
Microglia Participate in Neurogenic Regulation of Hypertension

Hypertension is associated with neuroinflammation and increased sympathetic tone. Interference with neuroinflammation by an anti-inflammatory reagent or overexpression of interleukin-10 in the brain was found to attenuate hypertension. However, the cellular mechanism of neuroinflammation, as well as its impact on neurogenic regulation of blood pressure, is unclear. Here, we found that hypertension, induced by either angiotensin II or L-NG-nitro-l-arginine methyl ester, is accompanied by microglial activation as manifested by microgliosis and proinflammatory cytokine upregulation. Targeted depletion of microglia significantly attenuated neuroinflammation, glutamate receptor expression in the paraventricular nucleus, plasma vasopressin level, kidney norepinephrine concentration, and blood pressure. Furthermore, when microglia were preactivated and transferred into the brains of normotensive mice, there was a significantly prolonged pressor response to intracerebroventricular injection of angiotensin II, and inactivation of microglia eliminated these effects. These data demonstrate that microglia, the resident immune cells in the brain, are the major cellular factors in mediating neuroinflammation and modulating neuronal excitation, which contributes to the elevated blood pressure.
Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by Phytophthora sojae effector Avr1b[W]

The sequenced genomes of oomycete plant pathogens contain large superfamilies of effector proteins containing the protein translocation motif RXLR-dEER. However, the contributions of these effectors to pathogenicity remain poorly understood. Here, we show that the Phytophthora sojae effector protein Avr1b can contribute positively to virulence and can suppress programmed cell death (PCD) triggered by the mouse BAX protein in yeast, soybean (Glycine max), and Nicotiana benthamiana cells. We identify three conserved motifs (K, W, and Y) in the C terminus of the Avr1b protein and show that mutations in the conserved residues of the W and Y motifs reduce or abolish the ability of Avr1b to suppress PCD and also abolish the avirulence interaction of Avr1b with the Rps1b resistance gene in soybean. W and Y motifs are present in at least half of the identified oomycete RXLR-dEER effector candidates, and we show that three of these candidates also suppress PCD in soybean. Together, these results indicate that the W and Y motifs are critical for the interaction of Avr1b with host plant target proteins and support the hypothesis that these motifs are critical for the functions of the very large number of predicted oomycete effectors that contain them.
Traitement des boues de vidange par lits de séchage sous climat soudano-sahélien

L'usage des lits de sechage plantes et non plantes (LSP et LSNP) pour le traitement des boues de vidange (BV) augmente en Afrique de l’Ouest malgre leurs performances parfois mauvaises et leur dimensionnement empirique. Pour contribuer a I'acces aux performances epuratoires des LSP et LSNP, le suivi de 13 lits de sechage experimentaux a ete realise. Sur la base de l'equation de consolidation de Terzaghi, un modele, permettant de simuler I'humidite volumique des boues sur les lits, a ete developpe selon 2 approches. Les resultats montrent des performances epuratoires mitigees. Pour des valeurs seuils de concentrations de BV, les 3 especes testees sur les LSP ont fletries. Les simulations avec l’Approche 1 presente des erreurs absolues relatives inferieures a 15% et sont meilleures que ceux avec l'approche 2. Cependant, I'approche 2 a l'avantage de reproduire les tendances de l'evolution des coefficients de consolidation et permeabilite des boues en fonction de la profondeur et du temps.
On the Error of Random Fourier Features

Kernel methods give powerful, flexible, and theoretically grounded approaches to solving many problems in machine learning. The standard approach, however, requires pairwise evaluations of a kernel function, which can lead to scalability issues for very large datasets. Rahimi and Recht (2007) suggested a popular approach to handling this problem, known as random Fourier features. The quality of this approximation, however, is not well understood. We improve the uniform error bound of that paper, as well as giving novel understandings of the embedding's variance, approximation error, and use in some machine learning methods. We also point out that surprisingly, of the two main variants of those features, the more widely used is strictly higher-variance for the Gaussian kernel and has worse bounds.
An approach for estimating code changes in e-commerce applications

E-commerce applications automate various business processes within an organization. To maintain a competitive edge, business analysts frequently modify business processes. Determining the effort for modifying a business process is not a trivial task, since the changes to the business process will result in changes to the source code for which the business analyst has limited knowledge. In this paper, we propose an approach for tracing the propagation of a change from the business process level to the code. We derive a change impact metric which estimates the code to be modified as a result of a business process change. A case study, using a large e-commerce application from the OFBiz open source project, demonstrates the effectiveness of our proposed change impact metric.
Commentary: BCG vaccination halves neonatal mortality.

Human enterovirus 68 (EV68) infections are rarely reported. We describe a respiratory outbreak associated with EV68 among 18 children admitted to a remote Indian Health Service facility during August 11, 2010 through September 14, 2010. Clinical illness was characterized by pneumonia and wheezing. EV68 should be considered as an etiology in outbreaks of lower respiratory tract illness. The Pediatric Infectious Disease Journal • Volume 31, Number 3, March 2012 BCG Halves Neonatal Mortality © 2012 Lippincott Williams & Wilkins www.pidj. com | 309
Dynamic IQC-Based Control of Uncertain LFT Systems With Time-Varying State Delay

This paper presents a new exact-memory delay control scheme for a class of uncertain systems with time-varying state delay under the integral quadratic constraint (IQC) framework. The uncertain system is described as a linear fractional transformation model including a state-delayed linear time-invariant (LTI) system and time-varying structured uncertainties. The proposed exact-memory delay controller consists of a linear state-feedback control law and an additional term that captures the delay behavior of the plant. We first explore the delay stability and the L2-gain performance using dynamic IQCs incorporated with quadratic Lyapunov functions. Then, the design of exact-memory controllers that guarantee desired L2-gain performance is examined. The resulting delay control synthesis conditions are formulated in terms of linear matrix inequalities, which are convex on all design variables including the scaling matrices associated with the IQC multipliers. The IQC-based exact-memory control scheme provides a novel approach for delay control designs via convex optimization, and advances existing control methods in two important ways: 1) better controlled performance and 2) simplified design procedure with less computational cost. The effectiveness and advantages of the proposed approach have been demonstrated through numerical studies.
CSF albumin and immunoglobulin analyses in childhood neurologic disorders

Objective: To evaluate the utility of qualitative and quantitative analyses of CSF immunoglobulins as part of the diagnostic workup of CNS inflammatory conditions. Methods: One hundred eighty-nine children who underwent CSF investigation for their neurologic condition had CSF and serum testing to (1) qualitatively identify oligoclonal band (OCB) patterns and (2) quantitatively measure the immunoglobulin (Ig) G index and albumin quotient (QAlb). Case notes were retrospectively reviewed and patients were grouped according to whether their primary diagnosis was due to an inflammatory (n = 104) or noninflammatory (n = 85) etiology. Results: CSF-restricted OCBs were found in 20/104 (19%) of the inflammatory group compared with 4/85 (5%) of the noninflammatory group (p= 0.0036). Mirrored OCBs were found in 13/104 (12.5%) of the inflammatory group compared with 5/85 (6%) of the noninflammatory group (p = 0.14). IgG index and QAlb were significantly higher in patients with an inflammatory etiology. However, a raised IgG index (>0.85) and QAlb (>0.049) were seen in both groups, with QAlb abnormalities seen more frequently in the inflammatory group (p = 0.0028). Conclusions: Both methods were informative in identifying inflammatory mechanisms. Abnormalities were more commonly, but not exclusively, seen in primary inflammatory conditions. The qualitative and quantitative evaluation collectively revealed additional positive results than when done in isolation.
Genome-Wide Profiling of MicroRNAs in Adipose Mesenchymal Stem Cell Differentiation and Mouse Models of Obesity

In recent years, there has been accumulating evidence that microRNAs are key regulator molecules of gene expression. The cellular processes that are regulated by microRNAs include e.g. cell proliferation, programmed cell death and cell differentiation. Adipocyte differentiation is a highly regulated cellular process for which several important regulating factors have been discovered, but still not all are known to fully understand the underlying mechanisms. In the present study, we analyzed the expression of 597 microRNAs during the differentiation of mouse mesenchymal stem cells into terminally differentiated adipocytes by real-time RT-PCR. In total, 66 miRNAs were differentially expressed in mesenchymal stem cell-derived adipocytes compared to the undifferentiated progenitor cells. To further study the regulation of these 66 miRNAs in white adipose tissue in vivo and their dependence on PPARγ activity, mouse models of genetically or diet induced obesity as well as a mouse line expressing a dominant negative PPARγ mutant were employed.
Evaluation of 3D-CPA, HR-HPV, and TCT joint detection on cervical disease screening

The application value of three-dimensional color power angiography (3D-CPA), high-risk human papillomavirus (HR-HPV), ThinPrep cytology test (TCT) joint detection on cervical disease screening was investigated. In total, 1,900 patients that were examined in Gynecological and Cervix Clinic of Maternal and Child Care Service Center of Xuzhou from June 2012 to March 2015 were enrolled in the present study. After admission, the patients underwent TCT, HR-HPV and 3D-CPA examinations, and vascular morphology and typing, vascularization index (VI) were recorded. Colposcopic biopsy was performed in patients with a positive outcome of any of the three indices. Pathological diagnosis was taken as the golden standard to assess the sensitivity, specificity, diagnostic rate, and Youden index of the three methods being used independently or jointly. Of the 1,900 patients, 276 cases (14.53%) were HR-HPV-positive, 214 cases (11.26%) were VI-positive and 164 cases (8.63%) were TCT-positive. A total of 418 cases were confirmed with a positive outcome of any of the three indices and a cervical biopsy was obtained. Of the 418 cases, 162 cases (38.75%) were diagnosed with chronic cervicitis, 146 cases with low-level cervical intraepithelial neoplasia (CIN) (34.93%), 104 cases (24.88%) with high level CIN, 6 cases (1.44%) with cervical cancer. Histology more than low level CIN was defined as positive: i) screening results when the three methods were used independently: HPV was confirmed with the highest sensitivity (90.63%), VI with the highest specificity (83.95%), and HPV with the highest diagnostic accuracy (83.73%); ii) screening results under HPV+TCT and HPV+TCT+VI: HPV+TCT+VI was confirmed with the highest sensitivity and specificity: sensitivity (94.53%), specificity (81.48%), diagnosis coincidence rate (89.47%) and the highest Youden index of 0.760; and iii) vascular morphology and grading were significantly different in the early stage cervical carcinoma, high level CIM, and cervicitis groups. In conclusion, the joint detection of 3D-CPA, HR-HPV, and TCT improved the sensitivity and accuracy of cervical disease screening. 3D-CPA technology may therefore be used as an auxiliary screening method for cervical cancer.
Hamiltonian chains in hypergraphs

Hamiltionian chain is a generalisation of hamiltonian cycles for hypergraphs. Among the several possible ways of generalisations this is probably the most strong one, it requires the strongest structure. Since there are many interesting questions about hamiltonian cycles in graphs, we can try to answer these questions for hypergraphs, too. In the present article we give a survey on results about such questions.
DNA methylation profiling to assess pathogenicity of BRCA1 unclassified variants in breast cancer

Germline pathogenic mutations in BRCA1 increase risk of developing breast cancer. Screening for mutations in BRCA1 frequently identifies sequence variants of unknown pathogenicity and recent work has aimed to develop methods for determining pathogenicity. We previously observed that tumor DNA methylation can differentiate BRCA1-mutated from BRCA1-wild type tumors. We hypothesized that we could predict pathogenicity of variants based on DNA methylation profiles of tumors that had arisen in carriers of unclassified variants. We selected 150 FFPE breast tumor DNA samples [47 BRCA1 pathogenic mutation carriers, 65 BRCAx (BRCA1-wild type), 38 BRCA1 test variants] and analyzed a subset (n=54) using the Illumina 450K methylation platform, using the remaining samples for bisulphite pyrosequencing validation. Three validated markers (BACH2, C8orf31, and LOC654342) were combined with sequence bioinformatics in a model to predict pathogenicity of 27 variants (independent test set). Predictions were compared with standard multifactorial likelihood analysis. Prediction was consistent for c.5194-12G>A (IVS 19-12 G>A) (P>0.99); 13 variants were considered not pathogenic or likely not pathogenic using both approaches. We conclude that tumor DNA methylation data alone has potential to be used in prediction of BRCA1 variant pathogenicity but is not independent of estrogen receptor status and grade, which are used in current multifactorial models to predict pathogenicity.
Influence of Urban Landscapes to Microclimatic Variances in a Tropical City

The outdoor spaces of hot-humid tropical cities are exposed directly to the high intensity of solar radiation. The increased heat gain contributes to the thermal discomfort in an urban environment. This study investigates the variability of outdoor air temperature produced on the different urban landscape morphology in Putrajaya. Results revealed that the temperature distribution is strongly influenced by the urban landscape morphology where significant temperature differences are observed at various urban areas. The findings could contribute toward a better understanding on the interrelationship between urban landscape morphology and its influence on the microclimatic atmosphere. Keywords: Urban landscape morphology; outdoor temperature; thermal variation; tropical city 2398-4295 © 2017 The Authors. Published for AMER ABRA by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, UniversitiTeknologi MARA, Malaysia.
Cellular Entry of Ebola Virus Involves Uptake by a Macropinocytosis-Like Mechanism and Subsequent Trafficking through Early and Late Endosomes

Zaire ebolavirus (ZEBOV), a highly pathogenic zoonotic virus, poses serious public health, ecological and potential bioterrorism threats. Currently no specific therapy or vaccine is available. Virus entry is an attractive target for therapeutic intervention. However, current knowledge of the ZEBOV entry mechanism is limited. While it is known that ZEBOV enters cells through endocytosis, which of the cellular endocytic mechanisms used remains unclear. Previous studies have produced differing outcomes, indicating potential involvement of multiple routes but many of these studies were performed using noninfectious surrogate systems such as pseudotyped retroviral particles, which may not accurately recapitulate the entry characteristics of the morphologically distinct wild type virus. Here we used replication-competent infectious ZEBOV as well as morphologically similar virus-like particles in specific infection and entry assays to demonstrate that in HEK293T and Vero cells internalization of ZEBOV is independent of clathrin, caveolae, and dynamin. Instead the uptake mechanism has features of macropinocytosis. The binding of virus to cells appears to directly stimulate fluid phase uptake as well as localized actin polymerization. Inhibition of key regulators of macropinocytosis including Pak1 and CtBP/BARS as well as treatment with the drug EIPA, which affects macropinosome formation, resulted in significant reduction in ZEBOV entry and infection. It is also shown that following internalization, the virus enters the endolysosomal pathway and is trafficked through early and late endosomes, but the exact site of membrane fusion and nucleocapsid penetration in the cytoplasm remains unclear. This study identifies the route for ZEBOV entry and identifies the key cellular factors required for the uptake of this filamentous virus. The findings greatly expand our understanding of the ZEBOV entry mechanism that can be applied to development of new therapeutics as well as provide potential insight into the trafficking and entry mechanism of other filoviruses.