Miracle-dz/UniFAR-FTUs huggingface dataset:
Flavopiridol Induces p53 via Initial Inhibition of Mdm2 and p21 and, Independently of p53, Sensitizes Apoptosis-Reluctant Cells to Tumor Necrosis Factor

Flavopiridol (FP) inhibits gene expression and causes apoptosis, and these effects cannot be explained by inhibition of cyclin-dependent kinases that govern cell cycle. The simple and established notion that FP is an inhibitor of transcription predicts its effects. Because Mdm-2 targets p53 for degradation, FP, as predicted, dramatically induced p53 by inhibiting Mdm-2. Once p53 was induced, restoration of transcription (by removal of FP) resulted in superinduction of p21 and Mdm-2. Similarly, low concentrations of FP (50 nm) induced p21 and Mdm-2 because of their initial down-regulation. A sustained decrease of Mdm-2/p21 expression and accumulation of p53 coincided with near-maximal cytotoxicity of FP at concentrations >100 nm. Induction of p53 was a marker, not a cause, of cytotoxicity. FP caused rapid apoptosis (caspase-dependent cell death) in p53-null leukemia cells. In these cells, FP-induced apoptosis was converted to growth arrest by inhibitors of caspases. In apoptosis-reluctant A549 and PC3M cancer cells, FP inhibited cell proliferation but did not cause apoptosis. Like typical inhibitors of transcription, FP sensitized cells to apoptotic stimuli, allowing tumor necrosis factor to cause rapid and massive apoptosis in otherwise apoptosis-reluctant cells. We discuss that, as a reversible inhibitor of transcription, FP can be used clinically in novel rational drug combinations.
How anatomy shapes dynamics: a semi-analytical study of the brain at rest by a simple spin model

Resting state networks (RSNs) show a surprisingly coherent and robust spatiotemporal organization. Previous theoretical studies demonstrated that these patterns can be understood as emergent on the basis of the underlying neuroanatomical connectivity skeleton. Integrating the biologically realistic DTI/DSI-(Diffusion Tensor Imaging/Diffusion Spectrum Imaging)based neuroanatomical connectivity into a brain model of Ising spin dynamics, we found a system with multiple attractors, which can be studied analytically. The multistable attractor landscape thus defines a functionally meaningful dynamic repertoire of the brain network that is inherently present in the neuroanatomical connectivity. We demonstrate that the more entropy of attractors exists, the richer is the dynamical repertoire and consequently the brain network displays more capabilities of computation. We hypothesize therefore that human brain connectivity developed a scale free type of architecture in order to be able to store a large number of different and flexibly accessible brain functions.
The precession of eccentric discs in close binaries

ABSTRA C T We consider the precession rates of eccentric discs in close binaries, and compare theoretical predictions with the results of numerical disc simulations and with observed superhump periods. A simple dynamical model for precession is found to be inadequate. For mass ratios &1/4 a linear dynamical model does provide an upper limit for disc precession rates. Theory suggests that pressure forces have a significant retrograde impact upon the precession rate. We find that the disc precession rates for three systems with accurately known mass ratios are significantly slower than predicted by the dynamical theory, and we attribute the difference to pressure forces. By assuming that pressure forces of similar magnitude occur in all superhumping systems, we obtain an improved fit to superhump observations.
RNA viruses and the mitogenic Raf/MEK/ERK signal transduction cascade

Abstract The Raf/MEK/ERK signal transduction cascade belongs to the mitogen-activated protein kinase (MAPK) cascades. Raf/MEK/ERK signaling leads to stimulus-specific changes in gene expression, alterations in cell metabolism or induction of programmed cell death (apoptosis), and thus controls cell differentiation and proliferation. It is induced by extracellular agents, including pathogens such as RNA viruses. Many DNA viruses are known to induce cellular signaling via this pathway. As these pathogens partly use the DNA synthesis machinery for their replication, they aim to drive cells into a proliferative state. In contrast, the consequences of RNA virus-induced Raf/MEK/ERK signaling were less clear for a long time, but since the turn of the century the number of publications on this topic has rapidly increased. Research on this virus/host-interaction will broaden our understanding of its relevance in viral replication. This important control center of cellular responses is differently employed to support the replication of several important human pathogenic RNA viruses including influenza, Ebola, hepatitis C and SARS corona viruses.
Spindle Self-organization and Cytokinesis During Male Meiosis in asterless Mutants of Drosophila melanogaster

While Drosophila female meiosis is anastral, both meiotic divisions in Drosophila males exhibit prominent asters. We have identified a gene we call asterless (asl) that is required for aster formation during male meiosis. Ultrastructural analysis showed that asl mutants have morphologically normal centrioles. However, immunostaining with antibodies directed either to γ tubulin or centrosomin revealed that these proteins do not accumulate in the centrosomes, as occurs in wild-type. Thus, asl appears to specify a function required for the assembly of centrosomal material around the centrioles. Despite the absence of asters, meiotic cells of asl mutants manage to develop an anastral spindle. Microtubules grow from multiple sites around the chromosomes, and then focus into a peculiar bipolar spindle that mediates chromosome segregation, although in a highly irregular way. Surprisingly, asl spermatocytes eventually form a morphologically normal ana–telophase central spindle that has full ability to stimulate cytokinesis. These findings challenge the classical view on central spindle assembly, arguing for a self-organization of this structure from either preexisting or newly formed microtubules. In addition, these findings strongly suggest that the asters are not required for signaling cytokinesis.
A novel high step-down interleaved converter with coupled inductor

Nowadays, high power density has become essential in networking, telecommunications and computing applications. Additionally, the electronic equipment used in these applications requires a very-low voltage feeding even when its power supply has a much higher voltage rating. Therefore, high step-down converters with high power density performance are required for these applications. Consequently, a novel two-phase interleaved high step-down converter is proposed in order to fulfill the requirements of high power density and high step-down conversion ratio of these applications. The proposed converter addresses the objective by a particular coupled inductor where three windings are only installed in one core. As a result, the proposed converter can achieve higher step-down ratio than the conventional topologies by adding a winding and two switches to the interleaved two phase buck converter, besides the coupled-inductor configuration. In this paper, the novel topology is introduced and analyzed in order to find its conversion ratio operation. Then, the proposed topology is compared to conventional topologies and some improved high step-down converters recently proposed. Finally, the proposed converter is experimentally validated and the results revealed that the proposed converter shows higher step-down conversion ratio than the conventional buck converter with a further increment of 40% in the conversion ratio when the converter is operating at a duty cycle of 30% and ratio of turns of 2.
Adaptive Word Sense Disambiguation Using Lexical Knowledge in Machine-readable Dictionary

This paper describes a general framework for adaptive conceptual word sense disambiguation. The proposed system begins with knowledge acquisition from machine-readable dictionaries. Central to the approach is the adaptive step that enriches the initial knowledge base with knowledge gleaned from the partial disambiguated text. Once the knowledge base is adjusted to suit the text at hand, it is applied to the text again to finalize the disambiguation decision. Definitions and example sentences from the Longman Dictionary of Contemporary English are employed as training materials for word sense disambiguation, while passages from the Brown corpus and Wall Street Journal (WSJ) articles are used for testing. An experiment showed that adaptation did significantly improve the success rate. For thirteen highly ambiguous words, the proposed method disambiguated with an average precision rate of 70.5% for the Brown corpus and 77.3% for the WSJ articles.
Empirical study on practices and performances of green purchasing among chinese manufacturing enterprises

As a key strategy for enterprises to implement environmental management, green purchasing has an important influence on companypsilas environmental performance and financial performance. Based on the surveys of 275 manufacturing enterprises, this paper identifies main factors of practice and performance on green purchasing through factor analysis using SPSS statistics software. And then, it analyzes data results through regression analysis. The conclusions and future research directions are suggested in the end.
A Luenberger-style Observer for Robot Manipulators with Position Measurements

This paper presents a novel Luenberger-style observer for robot manipulators with position measurements. Under the assumption that the state evolutions that are to be observed have bounded velocities, it is shown that the observer converges globally and exponentially. Comparisons and relations between the proposed observer and existing ones are discussed. The effectiveness of the observer is illustrated by a simulation for the Pendubot, an underactuated two-joint manipulator
Tissue-specific response of IGF-I mRNA expression to obesity-associated GH decline in the male Zucker fatty rat.

Diminished GH secretion is a well known association of obesity. As in obese humans, Zucker fatty rats develop a progressive GH deficiency, present at 6 weeks of age and maximal at 10 to 12 weeks. The aim of this study was to investigate the GH dependence of IGF-I gene expression in liver and extrahepatic tissues of the obese Zucker rat as a model of progressive GH reduction during adult life. Six- and 11-week-old obese Zucker rats and their lean littermates were used to compare body weight, glycemia, insulinemia, serum GH and IGF-I levels and IGF-I mRNA expression in liver, heart, aorta, kidney and skeletal muscle. In comparison with lean controls, obese Zucker rats showed at both ages comparable glycemia, severe hyperinsulinemia (mU/ml, mean+/-s.e.m.; 6 weeks 138+/-10 vs 45+/-6 P<0.001; 11 weeks 147+/-14 vs 46+/-3, P<0.001) and lower GH (ng/ml; 6 weeks 1.7+/-0.9 vs 2.7+/-1.1; 11 weeks 1.5+/-0.9 vs 4.2+/-1.2) in the presence of similar circulating IGF-I levels (ng/ml; 6 weeks 774+/-26 vs 694+/-28; 11 weeks 1439+/-182 vs 1516+/-121). Hepatic IGF-I mRNA expression was already reduced at 6 weeks of age due to a significant decrease in the IGF-Ib transcript compared with lean controls (relative units; IGF-Ia: 99+/-2% vs 100+/-5%; IGF-Ib: 69+/-10% vs 100+/-2%, P<0.05) and this reduction was more marked in 11-week-old animals when both IGF-I transcripts were significantly diminished (relative units; IGF-Ia: 80+/-6% vs 100+/-1%, P<0.05; IGF-Ib: 65+/-5% vs 100+/-2%, P<0.01). Extrahepatic tissues expressed almost exclusively the IGF-Ia transcript, the amount of which relative to controls was: (1) similar at 6 weeks and decreased at 11 weeks in kidney and skeletal muscle extracts (relative units; kidney: 6 weeks 88+/-10% vs 100+/-2%; 11 weeks 76+/-3% vs 100+/-4%, P<0.05; vastus lateralis: 6 weeks 95+/-7% vs 100+/-10%; 11 weeks 59+/-4% vs 100+/-2%, P<0.001); (2) similar at both ages in thoracic aorta (relative units; 6 weeks 121+/-6% vs 105+/-5%; 11 weeks: 91+/-14% vs 100+/-4%); and (3) increased at both ages in left ventricle extracts (relative units; 6 weeks 114+/-2% vs 99+/-9%, P<0. 05; 11 weeks 119+/-7% vs 95+/-3%, P<0.05). -specific dependence of IGF-I mRNA on GH levels during adulthood, reflected by the different behavior of IGF-I expression for each tissue in conditions of progressive decrease of GH levels.
Anti-counterfeiting using reflective micro structures - Based on random positioning of microstructures

We present a novel anti-counterfeiting and product security technique using a secure optical tag based on randomly distributed reflective micro particles. This technique can complement the existing security and logistics mechanisms like 1D/2D barcodes, DataMatrix, holograms etc. or can be used as a standalone solution too. The concept revolves around the use of random distribution of reflective microstructures as physically unclonable function (PUF). The particles are embedded into product surface or in the document. These particles are invisible to naked eye, the verification is done by imaging the reflections from these micro structures with a camera enabled cellphone equipped with some add-on optics. Here we present an overview of the system for using the secure optical tag, its registration and authentication.
Living In A Prototype: A Reconfigured Space

In this paper, we present a twenty-three months autobiographical design project of converting a Mercedes Sprinter van into a camper van. This project allows us to investigate the complexities and nuances of a case where people engage in a process of making, transforming and adapting a space they live in. This example opens a radically different and productive context for revisiting concepts that are currently at the center of human-computer interaction (HCI) research: ubiquitous computing, home automation, smart homes, and the Internet of Things. We offer six qualities characterizing the evolving relationship between the makers and the lived-in environment: the van. We conclude with a discussion on the two themes of living in a reconfigured home and prototype qualities in a reconfigured space, and a critical reflection around the theme of the invariably unfinished home.
Sphingopyxis chilensis sp. nov., a chlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate, and transfer of Sphingomonas alaskensis to Sphingopyxis alaskensis comb. nov.

The taxonomic position of a chlorophenol-degrading bacterium, strain S37T, was investigated. The 16S rDNA sequence indicated that this strain belongs to the genus Sphingopyxis, exhibiting high sequence similarity to the 16S rDNA sequences of Sphingomonas alaskensis LMG 18877T (98.8%), Sphingopyxis macrogoltabida LMG 17324T (98.2%), Sphingopyxis terrae IFO 15098T (95%) and Sphingomonas adhaesiva GIFU 11458T (92%). These strains (except Sphingopyxis terrae IFO 15098T, which was not investigated) and the novel isolate accumulated polyhydroxyalkanoates consisting of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from glucose as carbon source. The G + C content of the DNA of strain S37T was 65.5 mol%. The major cellular fatty acids of this strain were octadecenoic acid (18 : 1omega7c), heptadecenoic acid (17 : 1omega6c) and hexadecanoic acid (16 : 0). The results of DNA-DNA hybridization experiments and its physiological characteristics clearly distinguished the novel isolate from all known Sphingopyxis species and indicated that the strain represents a novel Sphingopyxis species. Therefore, the species Sphingopyxis chilensis sp. nov. is proposed, with strain S37T (=LMG 20986T =DSM 14889T) as the type strain. The transfer of Sphingomonas alaskensis to the genus Sphingopyxis as Sphingopyxis alaskensis comb. nov. is also proposed.
CIRCULAR MICROSTRIP SLOT ANTENNA FOR DUAL- FREQUENCY RFID APPLICATION

A compact wideband dual-frequency microstrip antenna is proposed in this paper. By employing an ofiset microstrip-fed line and a strip close to the radiating edges in the circular slot patch, an antenna operating at dual frequency with the impedance bandwidth of 26.2% and 22.2% respectively is presented. By attaching a strip to the radiating edges opposite to the microstrip-fed line, this alters the current distribution and radiation on the antenna at the second resonant frequency. The second frequency is also tunable by varying the lengths of the microstrip-fed line. It is demonstrated that the proposed antenna covers the widebands of UHF and microwave for RFID application. A good agreement is obtained between the simulated and experimental results.
Bacterial and fungal flora of dust deposits in a pig building.

OBJECTIVE--The purpose of the study was to investigate the bacterial and fungal flora of dust deposits in a newly built pig grower finisher building. Viable bacterial counts and microbial species found in a barn which had never housed pigs were compared with those in a barn housing 144 pigs. METHODS--The quantitative streak plate method was used to measure viable bacterial counts on nutrient agar or sheep blood agar. Viable bacterial counts of the dust deposits were expressed as the number of colony forming units (CFUs)/mg of dust. Gram positive cocci and Gram negative bacilli were identified by an automated system. Identifications with a confidence interval > 90% were accepted at the species level. Fungi were identified to the genus level with slide culture preparations on cereal agar. RESULTS--The lowest viable bacterial count (4.8 x 10(4)/mg of dust) was found in the barn with no pigs. In the barn with pigs the highest viable bacterial count (2.1 x 10(6)/mg of dust) was in dust from the top of a partition close to pig activity. Six species of bacteria or fungi were found in dust from the room with no pigs, whereas 22 different microorganisms were detected in dust from the room with pigs. With the exception of Enterobacter agglomerans no other species of the family Enterobacteriaceae was found in dust deposits in this new pig building. Twelve species of Gram positive bacteria were found in the room housing pigs. CONCLUSIONS--The pig is not only a source but also a disperser of airborne bacteria in pig buildings. Speciation of the microbial flora in dust from the pig building suggests that many of the microorganisms were either of human or environmental origin. Nevertheless as some of these microorganisms are known opportunistic pathogens or allergens and because of the documented increased incidence of chronic respiratory symptoms in pig workers, precautions to reduce inhalation of microbial or dust particles by pig workers seem prudent.
Does appraisal enhance learning, improve practice and encourage continuing professional development? A survey of general practitioners' experiences of appraisal.

BACKGROUND Revalidation of the medical profession is under review and a system has been proposed to ensure doctors meet standards of practice and professionalism. The current appraisal system allows clinicians to chart their progress and identify developmental needs in order to improve performance. Appraisal is now an annual compulsory requirement for all doctors. AIM AND OBJECTIVES The aim of the study was to investigate the experiences of general practitioners (GPs) of the current appraisal process. The specific objectives were to consider the impact the appraisal process had exerted on their learning, practice and individual continuing professional development (CPD). METHODS We employed a cross-sectional design using a postal questionnaire sent to all doctors who work as GPs (n = 385) in West Kent. MAIN RESULTS Questionnaires were returned from 71.7% of doctors (n = 276). The key findings obtained were that 47.5% (n = 131) of doctors stated that taking part in the appraisal process had enhanced their learning, 40.2% (n = 111) felt that the appraisal process had improved their practice and 55.8% (n = 154) stated that the appraisal process had encouraged their CPD. Qualitative findings derived from thematic analysis of open questions revealed that participants viewed the role of the appraiser as respected peer to be vital and there was a need for independence in the appraiser's appointment. The time-consuming nature of the appraisal process was emphasised, with little protected time for preparation of documentation and engagement in CPD. Concerns were expressed about links between appraisal and revalidation. CONCLUSIONS Many doctors considered that the appraisal process had enhanced their learning, improved their practice and encouraged their CPD. A vital, independent role for the appraiser was emphasised as was a need to review the time-consuming nature of the current appraisal process, together with identifying protected time to complete this and CPD engagement. As the role of appraisal within the revalidation process changes it is recognised that ensuring the quality, consistency and nature of appraisal will be essential to maintain the confidence of patients and doctors.
Retention and Nonretention of At-Risk Readers in First Grade and Their Subsequent Reading Achievement

Some of the specific reasons for the success or failure of retention in the area of reading were examined via an in-depth study of a small number of both at-risk retained students and comparably low skilled promoted children. Longitudinal data were collected as students went from first grade to either repeating first grade and through second grade, or from first through second grade. The children's development in phonemic awareness, spelling-sound knowledge, word recognition, and the ability to read words in their reading series was closely examined. In addition, the children's listening comprehension was examined, especially as it related to reading comprehension and intelligence. Findings suggested that children who finish first grade with poor word recognition skill may benefit from retention if they start the repeated year with increased phonemic awareness. But further growth in word recognition may be impaired in second grade unless spelling-sound knowledge continues to grow. Retention did not appear to facilitate listening comprehension to the extent it did word recognition, with the effect of further limiting improvement in reading comprehension.
Bayesian Model Selection for Support Vector Machines, Gaussian Processes and Other Kernel Classifiers

We present a variational Bayesian method for model selection over families of kernels classifiers like Support Vector machines or Gaussian processes. The algorithm needs no user interaction and is able to adapt a large number of kernel parameters to given data without having to sacrifice training cases for validation. This opens the possibility to use sophisticated families of kernels in situations where the small "standard kernel" classes are clearly inappropriate. We relate the method to other work done on Gaussian processes and clarify the relation between Support Vector machines and certain Gaussian process models.