Miracle-dz/UniFAR-FTUs huggingface dataset:
High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish

ABSTRACT Neural damage is a devastating outcome of physical trauma. The glia are one of the main effectors of neuronal repair in the nervous system, but the dynamic interactions between peripheral neurons and Schwann cells during injury and regeneration remain incompletely characterized. Here, we combine laser microsurgery, genetic analysis, high-resolution intravital imaging and lattice light-sheet microscopy to study the interaction between Schwann cells and sensory neurons in a zebrafish model of neurotrauma. We found that chronic denervation by neuronal ablation leads to Schwann-cell death, whereas acute denervation by axonal severing does not affect the overall complexity and architecture of the glia. Neuronal-circuit regeneration begins when Schwann cells extend bridging processes to close the injury gap. Regenerating axons grow faster and directionally after the physiological clearing of distal debris by the Schwann cells. This might facilitate circuit repair by ensuring that axons are guided through unoccupied spaces within bands of Büngner towards their original peripheral target. Accordingly, in the absence of Schwann cells, regenerating axons are misrouted, impairing the re-innervation of sensory organs. Our results indicate that regenerating axons use haptotaxis as a directional cue during the reconstitution of a neural circuit. These findings have implications for therapies aimed at neurorepair, which will benefit from preserving the architecture of the peripheral glia during periods of denervation. Summary: Schwann cells are important components of the peripheral glia. We use microsurgery and high-resolution live imaging to show how Schwann cells control the regeneration of a sensorineural circuit.
Analysis of Variance

In the following, analysis of variance (ANOVA or AOV) is illustrated for a case where there are k groups or regions, and , 1, i n i k   , observations in the -th i group or region. The model that underlies analysis of variance assumes that each observation has several components ( ) ( ), where the -th observation in the -th group or region, the grand mean (or all observations in all groups), ( ) is an "among (or between) groups" deviation (the diffe ij T i T ij i ij
Mobile phone text messaging in the management of diabetes

We conducted a trial of mobile phone text messaging (short message service; SMS) for diabetes management. In an eight-month period, 23 diabetic patients used the service. Patients used SMS to transmit data such as blood glucose levels and body weight to a server. The server automatically answered with an SMS acknowledgement message. A monthly calculated glycosylated haemoglobin result was also automatically sent to the patient by SMS. During the trial the patients sent an average of 33 messages per month. Although users showed good acceptance of the SMS diabetes system, they expressed various concerns, such as the inability to enter data from previous days. Nonetheless, the trial results suggest that SMS may provide a simple, fast and efficient adjunct to the management of diabetes. It was particularly useful for elderly persons and teenagers, age groups that are known to have difficulty in controlling their diabetes.
Effectively Identifying eQTLs from Multiple Tissues by Combining Mixed Model and Meta-analytic Approaches

Gene expression data, in conjunction with information on genetic variants, have enabled studies to identify expression quantitative trait loci (eQTLs) or polymorphic locations in the genome that are associated with expression levels. Moreover, recent technological developments and cost decreases have further enabled studies to collect expression data in multiple tissues. One advantage of multiple tissue datasets is that studies can combine results from different tissues to identify eQTLs more accurately than examining each tissue separately. The idea of aggregating results of multiple tissues is closely related to the idea of meta-analysis which aggregates results of multiple genome-wide association studies to improve the power to detect associations. In principle, meta-analysis methods can be used to combine results from multiple tissues. However, eQTLs may have effects in only a single tissue, in all tissues, or in a subset of tissues with possibly different effect sizes. This heterogeneity in terms of effects across multiple tissues presents a key challenge to detect eQTLs. In this paper, we develop a framework that leverages two popular meta-analysis methods that address effect size heterogeneity to detect eQTLs across multiple tissues. We show by using simulations and multiple tissue data from mouse that our approach detects many eQTLs undetected by traditional eQTL methods. Additionally, our method provides an interpretation framework that accurately predicts whether an eQTL has an effect in a particular tissue.
Cushing disease in children and adolescents: twenty years' experience in a tertiary care center in India.

OBJECTIVE To analyze the clinical presentation, diagnostic evaluation, treatment modalities, and follow-up of pediatric patients with Cushing disease. METHODS In this retrospective analysis, we reviewed records of children (younger than 20 years) with Cushing disease who had undergone transsphenoidal adenomectomy in a tertiary health care center in India during the period of 1988 to 2008. Endogenous hypercortisolism was identified by a serum cortisol value ≥1.8 μg/dL during a low-dose dexamethasone suppression test (LDDST) with or without elevated midnight serum cortisol (≥3.2 μg/dL). Corticotropin dependence was defined by a basal plasma corticotropin concentration ≥5 pg/mL. Patients with normal pituitary imaging underwent bilateral inferior petrosal sinus sampling (BIPSS). Those with persistent or recurrent disease after surgery were treated with second-line interventions on a case-by-case basis. RESULTS Twenty-nine boys and 19 girls were included. Mean age was 14.85 (±2.5) years. Weight gain (98%), round facies (98%), and growth arrest (83%) were the most common manifestations. LDDST and midnight cortisol had 100% sensitivity for detecting endogenous hypercortisolism, while midnight corticotropin measurement had 100% sensitivity for defining corticotropin dependence. Magnetic resonance imaging and unstimulated BIPSS had 71% and 89% sensitivity, respectively, for diagnosing Cushing disease. Twenty-seven patients (56%) achieved remission after the first transsphenoidal operation with higher remission rates in those with microadenoma (75%). Basal serum cortisol <5 mg/dL on the fifth postoperative day predicted cure. Eight patients received postoperative radiotherapy, with 4 achieving remission. CONCLUSIONS Clinical presentation and diagnostic yield with various tests were similar to those previously reported in the literature. Remission rates were poor after first transsphenoidal operation in patients with macroadenoma and outcome was dismal with a second transsphenoidal operation. Serum cortisol concentration <5 mg/dL on the fifth postoperative day predicted cure.
Q-MAM: a tool for solving infinite queues using matrix-analytic methods

In this paper we propose a novel MATLAB tool, called Q-MAM, to compute queue length, waiting time and so-journ time distributions of various discrete and continuous time queuing systems with an underlying structured Markov chain/process. The underlying paradigms include M/G/1-and GI/M/1-type, quasi-birth-death and non-skip-free Markov chains (implemented by the SMCSolver tool), as well as Markov processes with a matrix exponential distribution. We consider various single server queueing systems with phase-type, matrix exponential, Markovian, rational and semi-Markovian arrival and service processes; queues with multiple customer types, where the service depends on the customer type and where consecutive customer types may be correlated; and queues with multiple servers for which the typical dimensionality problem can be avoided. Apart from implementing various classical and more advanced solution techniques, the tool also extends and improves some of the existing solution techniques in a number of cases.
Artificial Light at Night and Cancer: Global Study

Background: Artificial light at night (ALAN) has been linked to increased risk of cancers in body sites like the breast and colorectum. However exposure of ALAN as an environmental risk factor and its relation to cancers in humans has never been studied in detail. Objective: To explore the association of ALAN with all forms of cancers in 158 countries. Materials and Methods: An ecological study encompassing global data was conducted from January to June 2015, with age-standardized rates (ASR) of cancers as the outcome measure. ALAN, in the protected areas, as the exposure variable, was measured with reference to the Protected Area Light Pollution Indicator (PALI) and the Protected Area Human Influence Indicator (PAHI). Pearson’s correlations were calculated for PALI and PAHI with ASR of cancers for 158 countries, adjusted for country populations, electricity consumption, air pollution, and total area covered by forest. Stratified analysis was conducted according to the country income levels. Linear regression was applied to measure the variation in cancers explained by PALI and PAHI. Results: PALI and PAHI were positively associated with ASR of all forms of cancer, and also the four most common cancers (p < 0.05). These positive correlations remained statistically significant for PAHI with all forms of cancer, lung, breast, and colorectal cancer after adjusting for confounders. Positive associations of PALI and PAHI with cancers varied with income level of the individual countries. Variation in all forms of cancers, and the four most common cancers explained by PALI and PAHI, ranged from 3.3 – 35.5%. Conclusion: Artificial light at night is significantly correlated for all forms of cancer as well as lung, breast, colorectal, and prostate cancers individually. Immediate measures should be taken to limit artificial light at night in the main cities around the world and also inside houses.
Estimating a financial distress rating system for Spanish firms with a simple hazard model

Under the Basel II Internal Rating Based (IRB) approach banks should accurately discriminate among different grades of obligors in their credit portfolios taking into account not only the obligor-specific characteristics but also their sector and macro-economic environment. The final objective is the assignment of a credit rating to each of their exposures based upon a set of estimated probabilities of default. With this purpose in mind, the industry has been gradually moving to more advanced techniques in financial distress prediction, with survival models playing a prominent role in the last few years.
Evaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species

CHROMagar Candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei. We evaluated this medium and compared it with a reference medium, Sabouraud glucose agar, for the presumptive identification of yeast species isolated directly on the medium from 1150 clinical specimens. A total of 731 specimens showed no growth, 299 isolates (70.2%) showed growth to the same extent on both media. Forty mixed cultures were detected on both media. More than one isolate was detected in 30 of the tested specimens on either CHROMagar (26 specimens) or Sabouraud glucose agar (four specimens). We found a sensitivity of 98.8% and a specificity of 100% for C. albicans, 66.7% and 99.8% for C. tropicalis, 100% and 100% for C. krusei, and 98% and 95.7% for C. glabrata. Regarding these results, CHROMagar Candida is recommended as a useful isolation medium capable of the presumptive identification of yeasts and better detection of mixed cultures in clinical specimens.
Two-level processor-sharing scheduling disciplines: mean delay analysis

Inspired by several recent papers that focus on scheduling disciplines for network flows, we present a mean delay analysis of Multilevel Processor Sharing (MLPS) scheduling disciplines in the context of M/G/1 queues. Such disciplines have been proposed to model the effect of the differentiation between short and long TCP flows in the Internet. Under MLPS, jobs are classified into classes depending on their attained service. We consider scheduling disciplines where jobs within the same class are served either with Processor Sharing (PS) or Foreground Background (FB) policy, and the class that contains jobs with the smallest attained service is served first. It is known that the FB policy minimizes (maximizes) the mean delay when the hazard rate of the job size distribution is decreasing (increasing). Our analysis, based on pathwise and meanwise arguments of the unfinished truncated work, shows that Two-Level Processor Sharing (TLPS) disciplines, e.g., FB+PS and PS+PS, are better than PS scheduling when the hazard rate of the job size distribution is decreasing. If the hazard rate is increasing and bounded, we show that PS outperforms PS+PS and FB+PS. We further extend our analysis to study local optimality within a level of an MLPS scheduling discipline.
Resilient Modulus and Strength of Base Course with Recycled Bituminous Material

The objective of the research was to determine the strength and deformation characteristics of base material produced from recycled asphalt pavement (RAP) and aggregate. Various samples with different ratios of RAP and aggregate base were mixed (% RAP/aggregate): 0/100, 25/75, 50/50, 75/25. Laboratory compaction testing and field monitoring indicated that gyratory compacted specimens were closer to the densities measured in the field. Resilient modulus (MR) tests were generally conducted following the National Cooperative Highway Research Program 1-28A test protocol. MR increased with increase of confining pressure, but MR showed little change with deviator stress. The specimens with 65% optimum moisture contents were stiffer than the specimens with 100% optimum moisture contents at all confining pressures. Cyclic triaxial tests were conducted at two deviator stresses, 35% and 50% of the estimated peak stress, to evaluate recoverable and permanent deformation behavior from initial loading to 5000 cycles. The specimens with RAP exhibited at least two times greater permanent deformation than the 100% aggregate material. As %RAP increased, more permanent deformation occurred. In summary, the base material produced with various %RAP content performed at a similar level to 100% aggregate in terms of MR and strength when properly compacted.
How does cognitive behaviour therapy reduce fatigue in patients with chronic fatigue syndrome? The role of physical activity

Background Cognitive behaviour therapy (CBT) is known to reduce fatigue severity in chronic fatigue syndrome (CFS). How this change in symptomatology is accomplished is not yet understood. The purpose of the present study was to determine whether the effect of CBT on fatigue is mediated by an increase in physical activity. Method Three randomized controlled trials were reanalysed, previously conducted to evaluate the efficacy of CBT for CFS. In all samples, actigraphy was used to assess the level of physical activity prior and subsequent to treatment or a control group period. The mediation hypothesis was analysed according to guidelines of Baron & Kenny [Journal of Personality and Social Psychology (1986)51, 1173–1182]. A non-parametric bootstrap approach was used to test statistical significance of the mediation effect. Results Although CBT effectively reduced fatigue, it did not change the level of physical activity. Furthermore, changes in physical activity were not related to changes in fatigue. Across the samples, the mean mediation effect of physical activity averaged about 1% of the total treatment effect. This effect did not yield significance in any of the samples. Conclusions The effect of CBT on fatigue in CFS is not mediated by a persistent increase in physical activity.
The supply side of housing …nance

We propose a new, data-based test for the presence of biased …nancial advice when households choose between Fixed Rate Mortgages (FRM) and Adjustable Rate Mortgages (ARM). If households are wary, the relative cost of FRM and ARM should be a su¢ cient statistic for a household contract choice: the identity of the bank originating the loan should play no role. If households rely on banks’ advice to guide their choice, banks may be tempted to bias advice in a direction that is most convenient to them, so that bank-speci…c characteristics play a role in the choice of household contract. We test this implication on the sample of 1.6 million mortgages originated in Italy between 2004 and 2010. We …nd that the choice between ARM
HMPAO brain SPECT in acute carbon monoxide poisoning.

UNLABELLED Technetium-99m-hexamethylpropylene amine oxime (HMPAO) brain images with fanbeam SPECT, in combination with surface three-dimensional display, were used to detect basal ganglion and cerebral cortex anomalies in the acute phase of carbon monoxide (CO) poisoning. METHODS Ten patients, aged 16-29 yr, with acute CO poisoning and no past history of neurologic disorders were enrolled in this study. After oxygen treatment, all 10 patients were investigated using 99mTc-HMPAO brain images with fanbeam SPECT and surface three-dimensional display. Meanwhile, 6 of 10 patients also received a brain CT scan. RESULTS CT scan findings were negative in all 6 patients. Fanbeam SPECT demonstrated unilateral or bilateral hypoactivity of basal ganglia in 6 patients. Local hypoactivity anomalies were found in the brain cortex of 7 patients, using surface three-dimensional display of the brain. Only 2 of 10 patients had normal 99mTc-HMPAO brain images. CONCLUSION This study suggests that, in comparison with traditional brain imaging techniques, 99mTc-HMPAO brain imaging with fanbeam SPECT in combination with surface three-dimensional display is a better tool for early detection of regional cerebral anomalies in acute CO poisoning.
Attitudes of Hungarian asthmatic and COPD patients affecting disease control: empirical research based on Health Belief Model

Introduction: Patient non-adherence to treatment is a major problem across most chronic diseases. In COPD and asthma treatments it is a complex issue because people need to make behavioral and lifestyle changes while taking medications. Poor adherence results in increased rates of morbidity and mortality, more frequent hospitalizations, and ultimately higher healthcare expenditures. Materials and methods: The objective of the study was to assess asthmatic and COPD patient's attitudes toward adherence in Hungary. Health Belief Model was used to help explain reasons of non-adherence. The results of the study should provide additional support to understanding health-related behaviors and to developing health related programs enhancing adherence of asthmatic and COPD patients. 145 diagnosed COPD patients and 161 diagnosed asthmatic patients were involved in 6 pulmonary centers. The questions were designed to measure Health Belief Model dimensions A 1–5 point verbal Likert scale was used. As a second stage, the answers were compared with the registered patient's personal health data available in pulmonary center's documentation. The data was analyzed using SPSS software. Results: More than 32% of patients are very interested in new asthma or COPD research results, but their main information source is physician. The trust toward the physician is very high. Patients accept treatments and rarely ask questions. Respondents are cooperative but sometimes fail to follow therapeutic recommendations. There is no willingness to join self-help groups or associations. Discussion: The paternalistic approach was generally accepted, moreover expected by the patients from the physicians. It is important to train patients, increase their self-efficacy, responsibility and involve them into self-management programs. Both physicians and patients should be trained how to communicate—this approach can lead to increased understanding and better adherence.
Genome-Wide Analysis of Sterol-Lipid Storage and Trafficking in Saccharomyces cerevisiae

ABSTRACT The pandemic of lipid-related disease necessitates a determination of how cholesterol and other lipids are transported and stored within cells. The first step in this determination is the identification of the genes involved in these transport and storage processes. Using genome-wide screens, we identified 56 yeast (Saccharomyces cerevisiae) genes involved in sterol-lipid biosynthesis, intracellular trafficking, and/or neutral-lipid storage. Direct biochemical and cytological examination of mutant cells revealed an unanticipated link between secretory protein glycosylation and triacylglycerol (TAG)/steryl ester (SE) synthesis for the storage of lipids. Together with the analysis of other deletion mutants, these results suggested at least two distinct events for the biogenesis of lipid storage particles: a step affecting neutral-lipid synthesis, generating the lipid core of storage particles, and another step for particle assembly. In addition to the lipid storage mutants, we identified mutations that affect the localization of unesterified sterols, which are normally concentrated in the plasma membrane. These findings implicated phospholipase C and the protein phosphatase Ptc1p in the regulation of sterol distribution within cells. This study identified novel sterol-related genes that define several distinct processes maintaining sterol homeostasis.
Melanocyte pigmentation inversely correlates with MCP‐1 production and angiogenesis‐inducing potential

The incidence of certain angiogenesis‐dependent diseases is higher in Caucasians than in African Americans. Angiogenesis is amplified in wound healing and cornea models in albino C57 mice compared with black C57 mice. Moreover, mouse and human melanocytes with low pigmentation stimulate endothelial cell (EC) proliferation and migration in vitro more than melanocytes with high pigmentation. This effect is due, in part, to the secretion of an angiogenic protein called fibromodulin (FMOD) from lowly pigmented melanocytes. Herein, we expand upon the mechanism contributing to increased angiogenesis in lighter skin and report that monocyte chemotactic protein‐1 (MCP‐1) is secreted by nonpigmented mouse melanocytes by 5‐ to 10‐fold more than pigmented melanocytes. MCP‐1 protein stimulates EC proliferation and migration in vitro and angiogenesis in vivo. Mechanistic studies determine that FMOD is upstream of MCP‐1 and promotes its secretion from both melanocytes and activated ECs via stimulation of NF‐κB activity. Mice injected with FMOD‐neutralizing antibodies show 2.3‐fold decreased levels of circulating MCP‐1. Human studies confirmed that, on average, Caucasians have 2‐fold higher serum levels of MCP‐1 than African Americans. Taken together, this study implicates the FMOD/MCP‐1 pathway in the regulation of angiogenesis by local melanocytes and suggests that melanogenic activity may protect against aberrant angiogenic diseases. —Adini, I. , Adini, A. , Bazinet, L. , Watnick, R. S. , Bielenberg, D. R. , and D'Amato, R. J. Melanocyte pigmentation inversely correlates with MCP‐1 production and angiogenesis‐inducing potential. FASEB J. 29, 662‐670 (2015). www.fasebj. org