Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of pre-and post-partum feed supplementation of Maraz does on the productive performance of their offspring

The purpose of this experiment was to investigate the impact of maternal pre-and post-partum feed supplementation, under farm conditions, on goat kid's growth, follicle traits and fiber production. The design of experiment was supplementation from 2 months pre-partum to 2 months post-partum with 3 nutritional groups (8 does each). The first does group (GI) was fed black barley at 250 g/doe/day (traditional farmer’s practice), whereas does of the second (GII) and third (GIII) groups were fed concentrate diet at 500 and 750 g/doe/day, respectively. Results revealed that birth weight from GII and GIII was significantly (P<0.05) higher by 22% (2.3 vs. 1.8 kg) than that of GI. Weaning weight and marketable live weight traits, growth rates, follicle traits except primary follicles, and fiber characteristics were significantly (P<0.05) higher in (GIII) followed by (GII) and then (GI). Moreover, goat kids of the GIII attained significantly (P<0.05) 37% (16.9 vs. 10.7 kg) and 26% (16.9 vs. 12.5 kg) more marketable live weight compared with GI and GII, respectively. Also, goat kids of the GIII produced 32% (813.3 vs. 553.3 g; P<0.05) and14% (813.3 vs. 703.3 g; P>0.05) higher fleece weight than that of GI and GII, respectively. Additionally, body weight traits, growth rates, and fiber characteristics were significantly (P<0.05) affected by sex of birth. In conclusion, supplementation with an increased level of feeding concentrate pre-and post-partum of Maraz goats showed a significant (P<0.05) positive effect on productive performance of their kids in comparison to the traditional farmer’s practice.
Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome

The population of India harbors one of the world’s most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine phosphorylation motifs (TPMs), in 32 H. pylori strains isolated from subjects with several forms of gastric diseases, was also explored. High resolution sequence analysis of the above described genes was performed. The nucleotide sequences obtained were translated into amino acids using MEGA (version 4.0) software for EPIYA. An MJ-Network was constructed for obtaining TPM haplotypes by using NETWORK (version 4.5) software. The findings of the study suggest that Indian H. pylori strains share a common ancestry with Europeans. No specific association of haplotypes with the outcome of disease was revealed through additional network analysis of TPMs.
RecA-Dependent DNA Repair Results in Increased Heteroplasmy of the Arabidopsis Mitochondrial Genome1[C][W]

Plant mitochondria have very active DNA recombination activities that are responsible for its plastic structures and that should be involved in the repair of double-strand breaks in the mitochondrial genome. Little is still known on plant mitochondrial DNA repair, but repair by recombination is believed to be a major determinant in the rapid evolution of plant mitochondrial genomes. In flowering plants, mitochondria possess at least two eubacteria-type RecA proteins that should be core components of the mitochondrial repair mechanisms. We have performed functional analyses of the two Arabidopsis (Arabidopsis thaliana) mitochondrial RecAs (RECA2 and RECA3) to assess their potential roles in recombination-dependent repair. Heterologous expression in Escherichia coli revealed that RECA2 and RECA3 have overlapping as well as specific activities that allow them to partially complement bacterial repair pathways. RECA2 and RECA3 have similar patterns of expression, and mutants of either display the same molecular phenotypes of increased recombination between intermediate-size repeats, thus suggesting that they act in the same recombination pathways. However, RECA2 is essential past the seedling stage and should have additional important functions. Treatment of plants with several DNA-damaging drugs further showed that RECA3 is required for different recombination-dependent repair pathways that significantly contribute to plant fitness under stress. Replication repair of double-strand breaks results in the accumulation of crossovers that increase the heteroplasmic state of the mitochondrial DNA. It was shown that these are transmitted to the plant progeny, enhancing the potential for mitochondrial genome evolution.
Herb-Induced Liver Injury in the Berlin Case-Control Surveillance Study

Herb-induced liver injury (HILI) has recently attracted attention due to increasing reports of hepatotoxicity associated with use of phytotherapeutics. Here, we present data on HILI from the Berlin Case-Control Surveillance Study. The study was initiated in 2000 to investigate the serious toxicity of drugs including herbal medicines. Potential cases of liver injury were ascertained in more than 180 Departments of all 51 Berlin hospitals from October 2002 to December 2011. Drug or herb intake was assessed through a standardized face-to-face interview. Drug or herbal aetiology was assessed based on the updated Council for International Organizations of Medical Sciences scale. In ten of all 198 cases of hepatotoxicity included in the study, herbal aetiology was assessed as probable (once ayurvedic herb) or possible (Valeriana five times, Mentha piperita once, Pelargonium sidoides once, Hypericum perforatum once, Eucalyptus globulus once). Mean age was 56.4 ± 9.7 years, and the predominant pattern of liver injury was hepatocellular. No cases of acute liver failure or death were observed. This case series corroborates known risks for ayurvedic herbs, supports the suspected association between Valeriana use and liver injury, and indicates a hepatotoxic potential for herbs such as Pelargonium sidoides, Hypericum perforatum or Mentha piperita that were rarely associated with liver injury before. However, given that possible causality does not prove clinical significance, further studies in this field are needed.
A CAD framework for co-design and analysis of CMOS-SET hybrid integrated circuits

This paper introduces a CAD framework for co-simulation of hybrid circuits containing CMOS and SET (Single Electron Transistor) devices. An improved analytical model for SET is also formulated and shown to be applicable in both digital and analog domains. Particularly, the extension of the recent MIB model for single/multi gate symmetric/asymmetric device for a wide range of drain to source voltage and temperature is addressed. Circuit level co-simulations are successfully performed by implementing the SET analytical model in Analog Hardware Description Language (AHDL) of a professional circuit simulator SMARTSPICE. Validation at device and circuit level is carried out by Monte-Carlo simulations. Some novel functionality hybrid CMOS-SET circuit characteristics: (i) SET neuron, (ii) Multiple valued logic circuit and (iii) a new Negative Differential Resistance (NDR) circuit, are also predicted by the proposed SET model and analyzed using the new hybrid simulator.
Prolonged Integration Site Selection of a Lentiviral Vector in the Genome of Human Keratinocytes

Background Lentiviral vectors have been successfully used for human skin cell gene transfer studies. Defining the selection of integration sites for retroviral vectors in the host genome is crucial in risk assessment analysis of gene therapy. However, genome-wide analyses of lentiviral integration sites in human keratinocytes, especially after prolonged growth, are poorly understood. Material/Methods In this study, 874 unique lentiviral vector integration sites in human HaCaT keratinocytes after long-term culture were identified and analyzed with the online tool GTSG-QuickMap and SPSS software. Results The data indicated that lentiviral vectors showed integration site preferences for genes and gene-rich regions. Conclusions This study will likely assist in determining the relative risks of the lentiviral vector system and in the design of a safe lentiviral vector system in the gene therapy of skin diseases.
Roles of catabolite activator protein sites centered at -81.5 and -41.5 in the activation of the Klebsiella aerogenes histidine utilization operon hutUH

The Klebsiella aerogenes hutUH operon is preceded by a promoter region, hut(P), that contains two divergent promoters (hutUp and Pc) which overlap and are alternately expressed. In the absence of the catabolite gene activator protein-cyclic AMP (CAP-cAMP) complex, Pc is predominantly expressed while hutUp is largely repressed. CAP-cAMP has the dual effect of repressing transcription from Pc while simultaneously activating transcription from hutUp. DNA deletion mutations in this region were used to identify DNA sequences required for transcription of these two promoters. We showed that inactivation of Pc by DNA deletion did not result in activation of hutUp in vitro or in vivo. In addition, Escherichia coli CAP mutants that are known to bind and bend DNA normally but are unable to activate various CAP-dependent promoters were also unable to activate hutUp in vivo. These results invalidate an indirect activation model by which CAP-mediated repression of Pc in itself would led to activation of hutUp. Gel retardation asays with various deletion mutations of hut(P) and DNase I protection analyses revealed a high-affinity CAP binding site (CAP site 1) centered at -81.5 relative to the hutUp start of transcription and a second low-affinity CAP site (CAP site 2) centered at about -41.5. CAP site 1 is essential for activation of hutUp. Although CAP site 2 by itself is unable to activate hutUp in vivo under catabolite-activating conditions, it appears to be required for maximal transcription from a site centered at -41.5, does not activate hutUp suggests that the role of CAP-cAMP at the weaker CAP site may be different from that of other promoters containing a similarly positioned site. We propose that CAP directly stimulates the activity of RNA polymerase at hutUp and that this reaction is completely dependent on a naturally occurring CAP site centered at -81.5 and also involves a second CAP site centered at about -41.5 for maximal activation.
Cultural Competency Training and Performance Reports to Improve Diabetes Care for Black Patients

BACKGROUND Increasing clinician awareness of racial disparities and improving communication may enhance diabetes care among black patients. OBJECTIVE To evaluate the effect of cultural competency training and performance feedback for primary care clinicians on diabetes care for black patients. DESIGN Cluster randomized, controlled trial conducted between June 2007 and May 2008. (ClinicalTrials.gov registration number: NCT00436176) SETTING: 8 ambulatory health centers in eastern Massachusetts. PARTICIPANTS 124 primary care clinicians caring for 2699 (36%) black and 4858 (64%) white diabetic patients. INTERVENTION INTERVENTION clinicians received cultural competency training and monthly race-stratified performance reports that highlighted racial differences in control of hemoglobin A(1c) (HbA(1c)) and low-density lipoprotein (LDL) cholesterol levels and blood pressure. MEASUREMENTS Clinician awareness of racial differences in diabetes care and rates of achieving clinical control targets among black patients at 12 months. RESULTS White and black patients differed significantly in baseline rates of achieving an HbA(1c) level less than 7% (46% vs. 40%), an LDL cholesterol level less than 2.59 mmol/L (<100 mg/dL) (55% vs. 43%), and blood pressure less than 130/80 mm Hg (32% vs. 24%) (all P < 0.050). At study completion, intervention clinicians were significantly more likely than control clinicians to acknowledge the presence of racial disparities in the 8 health centers as a whole (82% vs. 59%; P = 0.003), within their local health center (70% vs. 51%; P = 0.020), and among their own patients (63% vs. 43%; P = 0.037). Black patients of clinicians in the intervention and control groups did not differ at 12 months in rates of controlling HbA(1c) level (48% vs. 45%; P = 0.24), LDL cholesterol level (48% vs. 49%; P = 0.40), or blood pressure (23% vs. 25%; P = 0.47). LIMITATION 11% of primary care teams did not attend cultural competency training sessions. CONCLUSION The combination of cultural competency training and race-stratified performance reports increased clinician awareness of racial disparities in diabetes care but did not improve clinical outcomes among black patients.
N‐cadherin prodomain cleavage regulates synapse formation in vivo

Cadherins are initially synthesized bearing a prodomain that is thought to limit adhesion during early stages of biosynthesis. Functional cadherins lack this prodomain, raising the intriguing possibility that cells may utilize prodomain cleavage as a means to temporally or spatially regulate adhesion after delivery of cadherin to the cell surface. In support of this idea, immunostaining for the prodomain of zebrafish N‐cadherin revealed enriched labeling at neuronal surfaces at the soma and along axonal processes. To determine whether post‐translational cleavage of the prodomain affects synapse formation, we imaged Rohon‐Beard cells in zebrafish embryos expressing GFP‐tagged wild‐type N‐cadherin (NCAD‐GFP) or a GFP‐tagged N‐cadherin mutant expressing an uncleavable prodomain (PRON‐GFP) rendering it nonadhesive. NCAD‐GFP accumulated at synaptic microdomains in a developmentally regulated manner, and its overexpression transiently accelerated synapse formation. PRON‐GFP was much more diffusely distributed along the axon and its overexpression delayed synapse formation. Our results support the notion that N‐cadherin serves to stabilize pre‐ to postsynaptic contacts early in synapse development and suggests that regulated cleavage of the N‐cadherin prodomain may be a mechanism by which the kinetics of synaptogenesis are regulated. © 2009 Wiley Periodicals, Inc. Develop Neurobiol 2009
Antibiotic Stewardship Ward Rounds and a Dedicated Prescription Chart Reduce Antibiotic Consumption and Pharmacy Costs without Affecting Inpatient Mortality or Re-Admission Rates

Background Antibiotic consumption is a major driver of bacterial resistance. To address the increasing burden of multi-drug resistant bacterial infections, antibiotic stewardship programmes are promoted worldwide to rationalize antibiotic prescribing and conserve remaining antibiotics. Few studies have been reported from developing countries and none from Africa that report on an intervention based approach with outcomes that include morbidity and mortality. Methods An antibiotic prescription chart and weekly antibiotic stewardship ward round was introduced into two medical wards of an academic teaching hospital in South Africa between January-December 2012. Electronic pharmacy records were used to collect the volume and cost of antibiotics used, the patient database was analysed to determine inpatient mortality and 30-day re-admission rates, and laboratory records to determine use of infection-related tests. Outcomes were compared to a control period, January-December 2011. Results During the intervention period, 475.8 defined daily doses were prescribed per 1000 inpatient days compared to 592.0 defined daily doses/1000 inpatient days during the control period. This represents a 19.6% decrease in volume with a cost reduction of 35% of the pharmacy’s antibiotic budget. There was a concomitant increase in laboratory tests driven by requests for procalcitonin. There was no difference in inpatient mortality or 30-day readmission rate during the control and intervention periods. Conclusions Introduction of antibiotic stewardship ward rounds and a dedicated prescription chart in a developing country setting can achieve reduction in antibiotic consumption without harm to patients. Increased laboratory costs should be anticipated when introducing an antibiotic stewardship program.
MINDFULNESS, FAMILY RESILIENCE, AND PARENTING STRESS IN PARENTS WITH ELEMENTARY SCHOOL-AGE CHILDREN DURING THE COVID-19 PANDEMIC

The Covid-19 pandemic has impacts on life, especially for parents of school-age children. One of the phenomena that occur is parenting stress, which impacts parenting. Parenting stress requires the role of mindfulness and family resilience in order to be able to deal with stressful conditions of parenting during a pandemic. This study aimed to determine the relationship between mindfulness, family resilience, and parenting stress of parents who had school-age children. This quantitative study used a purposive sampling technique involving 60 parents with children of primary school age (93.3% female, Mage = 38; SD = 7.4). The instruments used in this research were the Mindful Attention Awareness Scale (MAAS) with (α = .911), Family Resilience Assessment Scale (α = .964), and The Parenting Stress Index (α = .994). The method of data analysis was multiple linear regression analysis. The result shows that mindfulness and family resilience are simultaneously statistically significant in predicting parenting stress, Adj R 2 = . 936, F(2, 58) = 428.89, p < . 001. In addition, it found that mindfulness does not contribute to the model, b = -. 025; t(58) = -. 686, p = . 495, while family resilience does, b = . 957; t(58) = 26.158, p < . 001. The effective contribution of mindfulness and family resilience to parenting stress is 93.6%. Based on the research results obtained, parents can develop mindfulness and family resilience to cope with the perceived stress of parenting.
Impact of headache on sickness absence and utilisation of medical services: a Danish population study.

STUDY OBJECTIVE--The aim was to study the extent and type of health service utilisation, medication habits, and sickness absence due to the primary headaches. DESIGN--This was a cross sectional epidemiological survey of headache disorders in a general population. Headache was diagnosed according to a structured interview and a neurological examination using the criteria of the International Headache Society. SETTING--A random sample of 25-64 year-old individuals was drawn from the Danish National Central Person Registry. All subjects were living in the Copenhagen County. PARTICIPANTS--740 subjects participated (76% of the sample); 119 had migraine and 578 had tension type headache. MAIN RESULTS--Among subjects with migraine 56% had, at some time, consulted their general practitioner because of the migraine. The corresponding percentage among subjects with tension type headache was 16. One or more specialists had been consulted by 16% of migraine sufferers and by 4% of subjects with tension type headache. The consultation rates of chiropractors and physiotherapists were 5-8%. Hospital admissions and supplementary laboratory investigations due to headache were rare (< 3%). Half of the migraine sufferers and 83% of subjects with tension type headache in the previous year had managed with at least one type of drug in the current year. Acetylsalicylic acid preparations and paracetamol were the most commonly used analgesics. Prophylaxis of migraine was used by 7%. In the preceding year 43% of employed migraine sufferers and 12% of employed subjects with tension type headache had missed one or more days of work because of headache. Most common was 1-7 days off work. The total loss of workdays per year due to migraine in the general population was estimated at 270 days per 1000 persons. For tension type headache the corresponding figure was 820. Women were more likely to consult a practitioner than men, whereas no significant sex difference emerged as regards absenteeism from work. CONCLUSIONS--The impact of the headache disorders on work performance in the general population is substantial, and the disorders merit increased attention.
Voice authentication using short phrases: Examining accuracy, security and privacy issues

This paper examines a novel security model for voice biometrics that decomposes the overall problem into bits of “biometric identity security,” bits of “knowledge security,” and bits of “traditional encryption security. ” This is the first paper to examine balancing security gained from text-dependent and text-independent voice biometrics under this model. Our formulation allows for text-dependent voice biometrics to address both what you know and who you are. A text-independent component is added to defeat replay attacks. Further, we experimentally examine an extension of the recently introduced Vaulted Voice Verification protocol and the security tradeoffs of adding these elements. We show that by mixing text-dependent with text-independent voice verification and by expanding the challenge-response protocol, Vaulted Voice Verification can preserve privacy while addressing the problematic issues of voice as a remote/mobile biometric identifier. The resulting model supports both authentication and key release with the matching taking place client side, where a mobile device may be used. This novel security model addresses a real and crucial problem: that of security on a mobile device.
Inventory of Single Oak Trees Using Object- Based Method on WorldView-2 Satellite Images and on Earth

Remote sensing provides data types and useful resources for forest mapping. Today,one of the most commonly used application in forestry is the identification of singletree and tree species compassion using object-based analysis and classification ofsatellite or aerial images. Forest data, which is derived from remote sensing methods,mainly focuses on the mass i.e. parts of the forest that are largely homogeneous, inparticular, interconnected) and plot-level data. Haft-Barm Lake is the case study whichis located in Fars province, representing closed forest in which oak is the valuablespecies. High Resolution Satellite Imagery of WV-2 has been used in this study. Inthis study, A UAV equipped with a compact digital camera has been used calibratedand modified to record not only the visual but also the near infrared reflection (NIR) ofpossibly infested oaks. The present study evaluated the estimation of forest parametersby focusing on single tree extraction using Object-Based method of classification witha complex matrix evaluation and AUC method with the help of the 4th UAV phantombird image in two distinct regions. The object-based classification has the highest andbest accuracy in estimating single-tree parameters. Object-Based classification methodis a useful method to identify Oak tree Zagros Mountains forest. This study confirmsthat using WV-2 data one can extract the parameters of single trees in the forest. An overall Kappa Index of Agreement (KIA) of 0.97 and 0.96 for each study site has been achieved. It is also concluded that while UAV has the potential to provide flexible and feasible solutions for forest mapping, some issues related to image quality still need to be addressed in order to improve the classification performance.