Miracle-dz/UniFAR-FTUs huggingface dataset:
In vivo detection of porcine reproductive and respiratory syndrome virus RNA by in situ hybridization at different times postinfection

We studied the distribution of porcine reproductive and respiratory syndrome virus (PRRSV) RNA in tissues by in situ hybridization at different times postinfection (p.i.). The probe used for in situ hybridization was prepared by reverse transcription of PRRSV RNA, followed by PCR amplification of the cDNA. The sequence amplified corresponded to 433 bp from PRRSV open reading frame 7, which is contained in the nucleocapsid protein gene and which is highly conserved in both European and American strains (H. Mardassi, L. Wilson, S. Mounir, and S. Dea, J. Clin. Microbiol. 32:2197-2203, 1994). An immunohistochemical technique was used to detect PRRSV antigen in tissue from virus-infected animals by using a monoclonal antibody specific for the PRRSV nucleocapsid protein (E.A. Nelson, J. Christopher-Hennings, T. Drew, G. Wensvoort, J.E. Collins, and D.A. Benfield, J. Clin. Microbiol. 31:3184-3189, 1993). The detection of PRRSV RNA was conducted in tissues of 6-week-old pigs that had been infected with one of three different field PRRSV isolates and collected at times ranging from 4 to 42 days p.i. Hybridization signals specific for PRRSV RNA were detected in lung, lymphoid tissues, alveolar macrophages (obtained by lavage at the time of necropsy), Peyer's patches, and kidney. The PRRSV-positive cells in these tissues appeared to be predominantly macrophages. In lung tissue we also obtained evidence suggesting the involvement of type II pneumocytes in the replication of PRRSV. During the acute period of infection there was a close correlation between the detection of RNA and the detection of nucleocapsid protein in individual cells. At later times p.i. (28 and 42 days p.i.), instead, more cells containing only PRRSV RNA than those containing PRRSV RNA and also expressing PRRSV nucleocapsid protein were detected. These results suggest that PRRSV RNA might persist in the tissues of infected animals for a longer time than PRRSV antigen expression.
Longevity and gray hair, baldness, facial wrinkles, and arcus senilis in 13,000 men and women: the Copenhagen City Heart Study.

BACKGROUND We have previously reported that men who look older than their contemporaries have a significantly higher risk for myocardial infarction. The purpose of this study was to investigate whether persons with pronounced aging signs such as graying of hair, baldness, or facial wrinkles are prone to a shorter life span compared to their contemporaries. METHODS In the Copenhagen City Heart Study comprising a random sample of 20,000 men and women, we also recorded, in addition to cardiovascular risk factors, data on signs of aging: extent of gray hair, baldness, facial wrinkles, and arcus senilis (corneal arcus). During 16 years of follow-up, 3,939 persons (1,656 women and 2,283 men) had died. The Cox regression model for proportional hazards, which included age as an explanatory variable, was used for descriptive analysis of the correlation between these aging signs and all-cause mortality. RESULTS We found no correlation between the mortality and the extent of graying of the hair, or baldness or facial wrinkles in either of the sexes, irrespective of age. A single exception was observed in a small subgroup of men with no gray hair. They had a slightly, but significantly, lower mortality than the rest [relative risk (RR) = . 81, 95% confidence interval (CI) . 67-. 98; p < . 05]. The presence of arcus senilis was significantly correlated with a shorter life span in women (RR = 1.25, 95% CI 1.08-1.46; p < .01). For men the same tendency was found, but the correlation was not statistically significant. CONCLUSION We conclude that the degrees of graying of the hair, baldness, and facial wrinkles are not predictive of a shorter life span in men and women in the Copenhagen City Heart Study.
The Strives, Struggles, and Successes of Women Diagnosed With ADHD as Adults

The objective of the study was to aid an understanding of women’s experiences of living with attention deficit hyperactivity disorder (ADHD), with special consideration of the role of stigma and gender-specific issues. Semistructured in-depth interviews were conducted with five women aged 32 to 50 years, all diagnosed with ADHD as adults. The interviews were analyzed in accordance with thematic analysis. The data analyses were centered around five core themes: (a) from unidentified childhood ADHD to adult diagnosis, (b) present main symptoms and challenges, (c) conflict between ADHD symptoms and gender norms and expectations, (d) stigma of ADHD: “People think it’s a fake disease,” and (e) managing ADHD symptoms and identifying strengths. Despite their difficulties, all participants are highly educated and employed, and differ from common portrayals of individuals with ADHD as observably hyperactive, disruptive, or globally impaired. The participants are reluctant about disclosure of their diagnosis, due to fear of negative judgment and lack of understanding from others. The findings highlight the importance of recognizing and targeting ADHD as a serious disorder that yields continuing, and even increasing, impairment in multiple areas into adulthood. Gender-specific issues of ADHD need to be examined further, particularly challenges associated with motherhood. Stigma and the conflict between ADHD symptoms and gender norms complicate women’s experiences of living with ADHD, and should be essential areas of focus in research, educational settings, and the media.
Structure of the MHV-rules lagrangian

Recently, a canonical change of field variables was proposed that converts the Yang-Mills Lagrangian into an MHV-rules Lagrangian, i.e. one whose tree level Feynman diagram expansion generates CSW rules. We solve the relations defining the canonical transformation, to all orders of expansion in the new fields, yielding simple explicit holomorphic expressions for the expansion coefficients. We use these to confirm explicitly that the three, four and five point vertices are proportional to MHV amplitudes with the correct coefficient, as expected. We point out several consequences of this framework, and initiate a study of its implications for MHV rules at the quantum level. In particular, we investigate the wavefunction matching factors implied by the Equivalence Theorem at one loop, and show that they may be taken to vanish in dimensional regularisation.
Who does what during a code review? Datasets of OSS peer review repositories

We present four datasets that are focused on the general roles of OSS peer review members. With data mined from both an integrated peer review system and code source repositories, our rich datasets comprise of peer review data that was automatically recorded. Using the Android project as a case study, we describe our extraction methodology, the datasets and their application used for three separate studies. Our datasets are available online at http://sdlab.naist. jp/reviewmining/.
Preparing to cross the research proposal threshold: A case study of two international doctoral students

ABSTRACT This paper presents a study of two international doctoral students’ perspectives on preparing to formally present the thesis proposal, which we conceptualise as a threshold in the PhD journey. They participated in a thesis writing group (TWG) that aimed to support international doctoral students to develop aspects of their scholarship in the early stages of their candidature. The case students reported feeling ‘stuck’ before joining the TWG run by the authors. After the writing group experience, they reported that they had gained confidence and developed the skills and knowledge required to prepare for their proposal presentation. Their perspectives were gathered through semi-structured interviews that were analysed using the conceptual framework of threshold concepts for doctoral learning. This small-scale study suggests that the collegial support provided by a TWG can be a powerful pedagogy enabling doctoral scholars to confidently negotiate crossing the thesis proposal threshold.
Speech processing and morphological development in Greek-speaking children

There is currently little knowledge about the development of morphology in relation to phonology and the speech processing system (speech input, speech output, and lexical representations). In this thesis a psycholinguistic approach was used to investigate the development of phonological and morphological skills in Greek speaking preschool age children. The central hypothesis is that the successful acquisition of phonological and morphological characteristics of a spoken language depends on the accuracy and efficiency of speech processing skills. This has been explored through a longitudinal normative study of speech and language development and two intervention single case studies of children with speech difficulties. Two groups of typically developing children aged 3;0-3;5 and 4;6-5;0 years respectively were assessed longitudinally at three assessment points six months apart. At each point published language assessments and experimental tasks were used. A number of morphological phenomena e.g. tense, possessive pronouns, irregular plural, were taken as the basis for experimental stimuli that reflected the morphological and phonological properties of interest. These stimuli were used in tasks of speech perception and word and nonword repetition, items being matched across tasks. The two intervention studies focused on the impact of training the production of phonological targets on the accuracy of morphological productions, and vice versa, as well as the effect of this training on broader speech and language processing skills. Significant processing similarities were found between phonological and morphological items, as were relationships between the two domains. Both intervention case studies indicated that as a result of targeting the accurate production of morphemes, generalization to the accurate production of phonological characteristics may occur; one case also supported the reverse effect. Overall, these results suggest that the morphological characteristics of spoken language are an integral part of lexical representations, a finding which has interesting implications for speech and language therapy practice.
Affective Priming Using Facial Expressions Modulates Liking for Abstract Art

We examined the influence of affective priming on the appreciation of abstract artworks using an evaluative priming task. Facial primes (showing happiness, disgust or no emotion) were presented under brief (Stimulus Onset Asynchrony, SOA = 20ms) and extended (SOA = 300ms) conditions. Differences in aesthetic liking for abstract paintings depending on the emotion expressed in the preceding primes provided a measure of the priming effect. The results showed that, for the extended SOA, artworks were liked more when preceded by happiness primes and less when preceded by disgust primes. Facial expressions of happiness, though not of disgust, exerted similar effects in the brief SOA condition. Subjective measures and a forced-choice task revealed no evidence of prime awareness in the suboptimal condition. Our results are congruent with findings showing that the affective transfer elicited by priming biases evaluative judgments, extending previous research to the domain of aesthetic appreciation.
The processing complexity of English relative clauses

Sentences that contain non-local dependencies between words have long been known to cause comprehension difficulty, relative to sentences where all the dependencies are local. Relative clause (RC) structures have been used extensively to evaluate hypotheses about the source of these complexity effects because different word order configurations can be compared while keeping the words the same and the meaning similar across conditions. The most frequently contrasted RC structures are subject-extracted and object-extracted RCs (SRCs and ORCs), the two most common types of RCs crosslinguistically (Keenan & Comrie, 1977). For example, consider the English RCs modifying the subject noun phrase (NP) “the reporter” in (1):
User cooperation diversity. Part II. Implementation aspects and performance analysis

For pt.I see ibid., p.1927-38. This is the second of a two-part paper on a new form of spatial diversity, where diversity gains are achieved through the cooperation of mobile users. Part I described the user cooperation concept and proposed a cooperation strategy for a conventional code-division multiple-access (CDMA) system. Part II investigates the cooperation concept further and considers practical issues related to its implementation. In particular, we investigate the optimal and suboptimal receiver design, and present performance analysis for the conventional CDMA implementation proposed in Part I. We also consider a high-rate CDMA implementation and a cooperation strategy when assumptions about the channel state information at the transmitters are relaxed. We illustrate that, under all scenarios studied, cooperation is beneficial in terms of increasing system throughput and cell coverage, as well as decreasing sensitivity to channel variations.
Intraflagellar Transport (IFT) Protein IFT25 Is a Phosphoprotein Component of IFT Complex B and Physically Interacts with IFT27 in Chlamydomonas

BACKGROUND Intraflagellar transport (IFT) is the bidirectional movement of IFT particles between the cell body and the distal tip of a flagellum. Organized into complexes A and B, IFT particles are composed of at least 18 proteins. The function of IFT proteins in flagellar assembly has been extensively investigated. However, much less is known about the molecular mechanism of how IFT is regulated. METHODOLOGY/PRINCIPAL FINDINGS We herein report the identification of a novel IFT particle protein, IFT25, in Chlamydomonas. Dephosphorylation assay revealed that IFT25 is a phosphoprotein. Biochemical analysis of temperature sensitive IFT mutants indicated that IFT25 is an IFT complex B subunit. In vitro binding assay confirmed that IFT25 binds to IFT27, a Rab-like small GTPase component of the IFT complex B. Immunofluorescence staining showed that IFT25 has a punctuate flagellar distribution as expected for an IFT protein, but displays a unique distribution pattern at the flagellar base. IFT25 co-localizes with IFT27 at the distal-most portion of basal bodies, probably the transition zones, and concentrates in the basal body region by partially overlapping with other IFT complex B subunits, such as IFT46. Sucrose density gradient centrifugation analysis demonstrated that, in flagella, the majority of IFT27 and IFT25 including both phosphorylated and non-phosphorylated forms are cosedimented with other complex B subunits in the 16S fractions. In contrast, in cell body, only a fraction of IFT25 and IFT27 is integrated into the preassembled complex B, and IFT25 detected in complex B is preferentially phosphorylated. CONCLUSION/SIGNIFICANCE IFT25 is a phosphoprotein component of IFT particle complex B. IFT25 directly interacts with IFT27, and these two proteins likely form a subcomplex in vivo. We postulate that the association and disassociation between the subcomplex of IFT25 and IFT27 and complex B might be involved in the regulation of IFT.
The Risk Need Responsivity Model of Offender Rehabilitation: Is There Really a Need for a Paradigm Shift?.

The current paper critically reviews the Risk-Need-Responsivity (RNR) and Good Lives Model (GLM) approaches to correctional treatment. Research, or the lack thereof, is discussed in terms of whether there is a need for a new model of offender rehabilitation. We argue that although there is a wealth of research in support of RNR approaches, there is presently very little available research demonstrating the efficacy of the GLM in terms of the impact that programs based on this model of rehabilitation have on observed rates of recidivism among offender populations. Additionally, the emphasis of the GLM approach on the principles and techniques of positive psychology is untested in the area of forensic psychology. Evidence with reference to the assessment and treatment of sexual offenders is discussed as this is a particular focus of the GLM approach. We conclude, in agreement with the developers of the GLM approach, that the RNR model needs to be adapted in order to incorporate recent research related to the factors associated with recidivism among sexual offenders. However, we argue that the GLM is largely an empirically untested model, and further offers little in terms of adding to or replacing the RNR model. We recognize that a revised version of the RNR based approach is necessary, incorporating an integrated approach to treatment, and we introduce and briefly describe the RNR-I (Integrated)), a model developed by the authors and supported by a variety of empirical research, including a number of outcome studies produced by our team and others.
Social anxiety and adolescence: Interpretation bias in an Italian Sample

Interpretation bias, namely the tendency to systematically assign a negative meaning to ambiguous social situations, has been extensively studied in adult populations, while in adolescents it has received little attention. We aimed to address this gap by analyzing the three components of interpretation bias (negative interpretations, positive interpretations, belief in negative interpretations) in Italian adolescents aged between 14 and 17, comparing two groups: high (n = 32) and average (n = 33) social anxiety. The participants filled Social Phobia Inventory (SPIN), Positive and Negative Affect Schedule (PANAS), and Adolescents’ Interpretation and Belief Questionnaire (AIBQ). We found that the bias is specific to social anxiety (not due to the presence of negative mood) and present only in social situations. Instead, contrary to the hypothesis, Italian adolescents with high social anxiety have both negative bias and absence of the positive bias. Hence, preventive interventions should focus not only on the reduction of negative interpretations. They should also reinforce positive interpretations and target belief in negative interpretations. Based on these considerations, we gave some suggestions to improve the tasks of the Cognitive Bias Modification addressed to adolescents. Finally, belief in negative interpretations and the relationship between negative and positive interpretations in social anxiety deserve further study, both in adolescents and in adults.
Genome architecture - number, size and length distributions of exons and introns in six crown eukaryotic genomes.

The genome profile of exon-intron distributions is presented for six eukaryotic genomes (H. sapiens, P. troglodytes, M. musculus, D. rerio, C. elegans, D. melanogaster) to deduce similarities and differences among their genome design and architecture. Interestingly, in all the six genomes, the total length in exons, introns and intergenic DNA on each chromosome is significantly correlated to the chromosome size. Another correlation is found between the total intron length and total exon length for each genome. The results presented provide a framework for understanding the nature and patterns of exon-intron length distributions, the constraints on them and their role in genome design and evolution and provide an insight into the evolution of intron-exon structures of eukaryotic genes.
The role of psychological states in predicting work engagement: A test of Kahn's model

THE ROLE OF PSYCHOLOGICAL STATES IN PREDICTING WORK ENGAGEMENT: A TEST OF KAHN’S MODEL by Taylor N. Gatti Researchers have consistently found engagement to be linked to positive individual and organizational outcomes such as job satisfaction, job performance, customer satisfaction, and productivity. Although task characteristics, transformational leadership, and core self-evaluations have been found to be important determinants of engagement, the mechanisms of why they are related to engagement are not well understood. Kahn (1990) argues that individuals become engaged through three psychological states: meaningfulness, safety, and availability. Using Kahn’s theory, the present study was conducted to test whether task characteristics, transformational leadership, and core self-evaluations were related to engagement through its respective psychological state. Data were collected from 114 full time and part time employees from various companies. Overall, psychological meaningfulness was found to mediate the relationship between each of the predictor variables and work engagement. These findings suggest that having a job that provides autonomy, task significance, task identity, skill variety, and feedback, having supervisors who motivate and inspire employees, and having a greater sense of self-esteem and self-efficacy, all make employees feel worthwhile and valued, which then impacts feelings of engagement. Organizations should strive to provide employees with an opportunity to use a variety of skills and autonomy, as well as train supervisors to display more transformational leadership characteristics.
Functional presynaptic HCN channels in the rat globus pallidus

Hyperpolarization‐activated, cyclic nucleotide‐gated cation (HCN) channels are expressed postsynaptically in the rodent globus pallidus (GP), where they play several important roles in controlling GP neuronal activity. To further elucidate the role of HCN channels in the GP, immunocytochemical and electrophysiological approaches were used to test the hypothesis that HCN channels are also expressed presynaptically on the local axon collaterals of GP neurons. At the electron microscopic level, immunoperoxidase labelling for HCN1 and HCN2 was localized in GP somata and dendritic processes, myelinated and unmyelinated axons, and axon terminals. One population of labelled terminals formed symmetric synapses with somata and proximal dendrites and were immunoreactive for parvalbumin, consistent with the axon collaterals of GABAergic GP projection neurons. In addition, labelling for HCN2 and, to a lesser degree, HCN1 was observed in axon terminals that formed asymmetric synapses and were immunoreactive for the vesicular glutamate transporter 2. Immunogold labelling demonstrated that HCN1 and HCN2 were located predominantly at extrasynaptic sites along the plasma membrane of both types of terminal. To determine the function of presynaptic HCN channels in the GP, we performed whole‐cell recordings from GP neurons in vitro. Bath application of the HCN channel blocker ZD7288 resulted in an increase in the frequency of mIPSCs but had no effect on their amplitude, implying that HCN channels tonically regulate the release of GABA. Their presence, and predicted role in modulating transmitter release, represents a hitherto unidentified mechanism whereby HCN channels influence the activity of GP neurons.