Miracle-dz/UniFAR-FTUs huggingface dataset:
Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range

Light-induced toxicity is a fundamental bottleneck in microscopic imaging of live embryos. In this article, after a review of photodamage mechanisms in cells and tissues, we assess photo-perturbation under illumination conditions relevant for point-scanning multiphoton imaging of live Drosophila embryos. We use third-harmonic generation (THG) imaging of developmental processes in embryos excited by pulsed near-infrared light in the 1.0–1.2 µm range. We study the influence of imaging rate, wavelength, and pulse duration on the short-term and long-term perturbation of development and define criteria for safe imaging. We show that under illumination conditions typical for multiphoton imaging, photodamage in this system arises through 2- and/or 3-photon absorption processes and in a cumulative manner. Based on this analysis, we derive general guidelines for improving the signal-to-damage ratio in two-photon (2PEF/SHG) or THG imaging by adjusting the pulse duration and/or the imaging rate. Finally, we report label-free time-lapse 3D THG imaging of gastrulating Drosophila embryos with sampling appropriate for the visualisation of morphogenetic movements in wild-type and mutant embryos, and long-term multiharmonic (THG-SHG) imaging of development until hatching.
Guidelines on the diagnosis and treatment of acute heart failure — full

ESC Committee for Practice Guidelines (CPG), Silvia G. Priori (Chairperson) (Italy), Maria Angeles Alonso Garcia (Spain), Jean-Jacques Blanc (France), Andrzej Budaj (Poland), Martin R. Cowie (UK), Veronica Dean (France), Jaap Deckers (The Netherlands), Enrique Fernandez Burgos (Spain), John Lekakis (Greece), Bertil Lindahl (Sweden), Gianfranco Mazzotta (Italy), João Morais (Portugal), Ali Oto (Turkey), Otto A. Smiseth (Norway)
Characterisation of uveitis in patients with psoriatic arthritis

OBJECTIVE The purpose of this study is to describe the clinical characteristics of uveitis related to psoriatic arthritis (PsA), and also to compare the uveitis in PsA to the uveitis in spondyloarthropathy (SA). METHODS Sixteen patients with uveitis and PsA were evaluated in a tertiary care uveitis clinic. These patients were compared retrospectively to a series of 89 patients with uveitis and SA. RESULTS Eight (50%) of the 16 patients with uveitis had strictly peripheral arthritis, while two (12.5%) had axial only, and six (37.5%) had axial and peripheral arthritis. Patients with uveitis and axial disease were more likely to be male (100% v 38%) and HLA-B27 positive (6 of 6 typed positive v 0 of 3 typed positive) when compared with those with uveitis and peripheral arthritis only. Compared with patients with SA, those with PsA were more likely to have insidious onset (19% v3%), simultaneously bilateral (37.5% v7%), chronic duration (31% v 6%), or posterior (44% v 17%) uveitis. Complications of uveitis were similar in the SA and PsA groups. CONCLUSION Uveitis in patients with PsA was more likely to be insidious in onset, continuous, posterior, and active bilaterally compared with uveitis in patients with SA. Patients with uveitis and axial involvement were more likely to be male and HLA-B27 positive compared with patients with uveitis and peripheral arthritis alone. Patients with seronegative arthritis and uveitis that begins insidiously, lasts longer than six months, is bilateral, or is posterior, should be carefully questioned about the presence of either psoriasis or inflammatory bowel disease.
Non-Motor Off Symptoms in Parkinson's Disease

The aim of this study is to elucidate the clinical spectrum and frequency of non-motor symptoms during off periods (NMOS) in Parkinson's disease (PD) patients with motor fluctuation. We compared clinical characteristics between PD patients with motor symptoms only (M-off) and those with both motor and non-motor symptoms (NM-off) during off periods. The association of NMOS with parkinsonian clinical characteristics was also investigated. Sixty-seven consecutive PD patients of both M-off and NM-off groups were included in this study. We reviewed medical records, interviewed the patients, and administered a structured questionnaire. NMOS is classified into three categories: autonomic, neuropsychiatric and sensory. The frequency of NMOS and their individual manifestations were assessed. Of 67 patients with off symptoms, 20 were M-off group and 47 NM-off group. Among NMOS, diffuse pain was the most common manifestation, followed by anxiety and sweating. There were no significant differences between M-off and NM-off groups with regard to age, duration of disease and treatment, interval between onset of parkinsonian symptoms and off symptoms and off periods. Patients taking higher dosage of levodopa had fewer NMOS. NMOS is frequent in PD. Comprehensive recognition of NMOS can avoid unnecessary tests and is important for optimal treatment in PD.
Learning to segment moving objects in videos

We segment moving objects in videos by ranking spatio-temporal segment proposals according to “moving objectness”; how likely they are to contain a moving object. In each video frame, we compute segment proposals using multiple figure-ground segmentations on per frame motion boundaries. We rank them with a Moving Objectness Detector trained on image and motion fields to detect moving objects and discard over/under segmentations or background parts of the scene. We extend the top ranked segments into spatio-temporal tubes using random walkers on motion affinities of dense point trajectories. Our final tube ranking consistently outperforms previous segmentation methods in the two largest video segmentation benchmarks currently available, for any number of proposals. Further, our per frame moving object proposals increase the detection rate up to 7% over previous state-of-the-art static proposal methods.
Dynamic knee joint mechanics after anterior cruciate ligament reconstruction.

PURPOSE There is scarcity of information on the long-term adaptations in lower limb biomechanics during game-specific movements after anterior cruciate ligament (ACL) reconstruction. Particularly, variables such as knee abduction moments and transverse plane knee motion have not been studied during a game-specific landing and cutting task after ACL reconstruction. The purpose of this study was to compare the hip and knee mechanics between the ACL-reconstructed (ACLr) group and a healthy control group. METHODS Thirty-eight reconstructed athletes (18 ACLr, 18 control) participated in the study. Three-dimensional hip, knee, and ankle angles were calculated during a maximal drop jump land from a 0.30-m box and unanticipated cutting task at 45°. RESULTS During the landing phase, ACLr participants had increased hip flexion (P < 0.003) and transverse plane knee range of motion (P = 0.027). During the cutting phase, the ACLr participant's previously injured limb had increased internal knee abduction moment compared with that of the control group (P = 0.032). No significant differences were reported between the previously injured and contralateral uninjured limb. CONCLUSIONS Previously injured participants demonstrated higher knee abduction moment and transverse plane range of motion when compared with those of control participants during a game-specific landing and cutting task.
Revision History

Active 27-001 Making newline locale aware Active 27-002 is_whitespace is inconsistent Active 27-004 example of changing the behavior of is_whitespace is incorrect. Active 27-005 not_eof specification Active 27-007 ios_traits typedefs are ‘char’ oriented Active 27-008 ios_traits::length is missing Returns: clause Active 27-009 ios_traits::get_state should be specified Active 27-010 ios_traits::get_pos should be specified Active 27-011 Return type for ios_traits::copy is incorrect
Connectivity in dense networks confined within right prisms

We consider the probability that a dense wireless network confined within a given convex geometry is fully connected. We exploit a recently reported theory to develop a systematic methodology for analytically characterizing the connectivity probability when the network resides within a convex right prism, a polyhedron that accurately models many geometries that can be found in practice. To maximize practicality and applicability, we adopt a general point-to-point link model based on outage probability, and present example analytical and numerical results for a network employing 2 × 2 multiple-input multiple-output (MIMO) maximum ratio combining (MRC) link level transmission confined within particular bounding geometries. Furthermore, we provide suggestions for extending the approach detailed herein to more general convex geometries.
Neurobiology of Alcohol Dependence

Alcoholism is a debilitating disorder for the individual and very costly for society. A major goal of alcohol research is to understand the neural underpinnings associated with the transition from alcohol use to alcohol dependence. Positive reinforcement is important in the early stages of alcohol use and abuse. Negative reinforcement can be important early in alcohol use by people self-medicating coexisting affective disorders, but its role likely increases following the transition to dependence. Chronic exposure to alcohol induces changes in neural circuits that control motivational processes, including arousal, reward, and stress. These changes affect systems utilizing the signaling molecules dopamine, opioid peptides, γ-aminobutyric acid, glutamate, and serotonin, as well as systems modulating the brain’s stress response. These neuroadaptations produce changes in sensitivity to alcohol’s effects following repeated exposure (i.e., sensitization and tolerance) and a withdrawal state following discontinuation of alcohol use. Chronic alcohol exposure also results in persistent neural deficits, some of which may fully recover following extended periods of abstinence. However, the organism remains susceptible to relapse, even after long periods of abstinence. Recent research focusing on brain arousal, reward, and stress systems is accelerating our understanding of the components of alcohol dependence and contributing to the development of new treatment strategies.
Remodeling of Autogenous Bone Grafts after Osteotome Sinus Floor Elevation Assessed by Limited Cone Beam Computed Tomography

This study assessed the radiographic appearance of bone graft domes longitudinally after osteotome sinus floor elevation using cone beam computed tomography (CBCT). This study presents the radiological findings of a 6-month follow-up CBCT study in maxillary osteotome sinus floor elevation. We examined 52 patients with a crestal bone height of less than 8 mm in the posterior maxilla who required sinus augmentation. Implants (n = 91) were subsequently placed in regenerated bone following osteotome sinus floor elevation; autogenous bone was used as the augmentation material. In all cases, the grafted augmentation material tended to be absorbed, but at least 1 mm of grafted augmentation material was recognized around the implant fixtures on CBCT at the second implant operation. The border between the grafted augmentation material and the existing bone was indistinct. The grafted area apical to the implants undergoes shrinkage and remodeling. It was suggested that sufficient grafted autogenous bone changes into bone to support an implant.
Attentive documents: Eye tracking as implicit feedback for information retrieval and beyond

Reading is one of the most frequent activities of knowledge workers. Eye tracking can provide information on what document parts users read, and how they were read. This article aims at generating implicit relevance feedback from eye movements that can be used for information retrieval personalization and further applications. We report the findings from two studies which examine the relation between several eye movement measures and user-perceived relevance of read text passages. The results show that the measures are generally noisy, but after personalizing them we find clear relations between the measures and relevance. In addition, the second study demonstrates the effect of using reading behavior as implicit relevance feedback for personalizing search. The results indicate that gaze-based feedback is very useful and can greatly improve the quality of Web search. The article concludes with an outlook introducing attentive documents keeping track of how users consume them. Based on eye movement feedback, we describe a number of possible applications to make working with documents more effective.
An Efficient Message Authentication Scheme for Vehicular Communications

In this paper, we introduce a novel roadside unit (RSU)-aided message authentication scheme named RAISE, which makes RSUs responsible for verifying the authenticity of messages sent from vehicles and for notifying the results back to vehicles. In addition, RAISE adopts the k- anonymity property for preserving user privacy, where a message cannot be associated with a common vehicle. In the case of the absence of an RSU, we further propose a supplementary scheme, where vehicles would cooperatively work to probabilistically verify only a small percentage of these message signatures based on their own computing capacity. Extensive simulations are conducted to validate the proposed scheme. It is demonstrated that RAISE yields a much better performance than previously reported counterparts in terms of message loss ratio (LR) and delay.
Chisel: Constructing hardware in a Scala embedded language

In this paper we introduce Chisel, a new hardware construction language that supports advanced hardware design using highly parameterized generators and layered domain-specific hardware languages. By embedding Chisel in the Scala programming language, we raise the level of hardware design abstraction by providing concepts including object orientation, functional programming, parameterized types, and type inference. Chisel can generate a high-speed C++-based cycle-accurate software simulator, or low-level Verilog designed to map to either FPGAs or to a standard ASIC flow for synthesis. This paper presents Chisel, its embedding in Scala, hardware examples, and results for C++ simulation, Verilog emulation and ASIC synthesis.
Morale in the English mental health workforce: questionnaire survey

Background High-quality evidence on morale in the mental health workforce is lacking. Aims To describe staff well-being and satisfaction in a multicentre UK National Health Service (NHS) sample and explore associated factors. Method A questionnaire-based survey (n = 2258) was conducted in 100 wards and 36 community teams in England. Measures included a set of frequently used indicators of staff morale, and measures of perceived job characteristics based on Karasek's demand–control–support model. Results Staff well-being and job satisfaction were fairly good on most indicators, but emotional exhaustion was high among acute general ward and community mental health team (CMHT) staff and among social workers. Most morale indicators were moderately but significantly intercorrelated. Principal components analysis yielded two components, one appearing to reflect emotional strain, the other positive engagement with work. In multilevel regression analyses factors associated with greater emotional strain included working in a CMHT or psychiatric intensive care unit (PICU), high job demands, low autonomy, limited support from managers and colleagues, age under 45 years and junior grade. Greater positive engagement was associated with high job demands, autonomy and support from managers and colleagues, Black or Asian ethnic group, being a psychiatrist or service manager and shorter length of service. Conclusions Potential foci for interventions to increase morale include CMHTs, PICUs and general acute wards. The explanatory value of the demand–support–control model was confirmed, but job characteristics did not fully explain differences in morale indicators across service types and professions.
Trust in Online Advice

Many people are now influenced by the information and advice they find on the Internet, much of it of dubious quality. This article describes two studies concerned with those factors capable of influencing people’s response to online advice. The first study is a qualitative account of a group of house-hunters attempting to find worthwhile information online. The second study describes a survey of more than 2,500 people who had actively sought advice over the Internet. A framework for understanding trust in online advice is proposed in which first impressions are distinguished from more detailed evaluations. Good web design can influence the first process, but three key factors—source credibility, personalization, and predictability—are shown to predict whether people actually follow the advice given.
Cross-Border Bank Mergers: What Lures the Rare Animal?

Although domestic mergers and acquisitions (M&As) in the financial services industry have increased steadily over the past two decades, international M&As were relatively rare until recently. This paper uses a novel dataset of over 2,300 mergers that took place between 1978 and 2001 to analyse the determinants of international bank mergers. We test the extent to which information costs and regulations hold back merger activity. Our results suggest that banks operating in more regulated environments are less likely to be the targets of international bank mergers. Hence, the lifting of regulations can spur growth in cross-border bank mergers. Also, mergers tend to be less frequent if information costs are high.
Some Inequalities in Functional Analysis, Combinatorics, and Probability Theory

The main purpose of this paper is to show that many inequalities in functional analysis, probability theory and combinatorics are immediate corollaries of the best approximation theorem in inner product spaces. Besides, as applications of the de Caen-Selberg inequality, the finite field Kakeya and Nikodym problems are also studied.
Identification of metabolites of octamethylcyclotetrasiloxane (D(4)) in rat urine.

Octamethylcyclotetrasiloxane (D(4)) is an industrial chemical of significant commercial importance. In this study, its major urinary metabolites were identified. The urine samples described here were collected from male and female Fischer rats (F-344) administered [(14)C]D(4) i.v. The metabolite profile was obtained using an HPLC system equipped with a radioisotope detector. HPLC analysis was performed on a C18 column, using an acetonitrile/water mobile phase. The HPLC radiochromatogram revealed two major and at least five minor metabolites. The two major metabolites, constituting 75 to 85% of the total radioactivity, were identified as dimethylsilanediol [Me(2)Si(OH)(2)] and methylsilanetriol [MeSi(OH)(3)]. Formation of MeSi(OH)(3) clearly established demethylation at the silicon-methyl bonds of D(4). No parent D(4) was present in urine. The minor metabolites identified were tetramethyldisiloxane-1,3-diol [Me(2)Si(OH)-O-Si(OH)Me(2)], hexamethyltrisiloxane-1,5-diol [Me(2)Si(OH)-OSiMe(2)-OSi(OH)Me(2)], trimethyldisiloxane-1,3,3-triol [MeSi(OH)(2)-O-Si(OH)Me(2)], dimethyldisiloxane-1,1,3,3-tetrol [MeSi(OH)(2)-O-Si(OH)(2)Me], and dimethyldisiloxane-1,1,1,3,3-pentol [Si(OH)(3)-O-Si(OH)(2)Me]. The structural assignments were based on gas chromatography-mass spectrometry analysis of the tetrahydrofuran metabolite extracts, which were derivatized using bis(trimethylsiloxy)triflouroacetamide, a trimethylsilylating agent. The structures were confirmed by synthesizing (14)C-labeled standards and comparing their HPLC radiochromatograms with the corresponding components in the rat urine. GC-MS spectral comparisons of the trimethylsilylated derivatized standards and urinary components also were made to further confirm their identities. Finally, several of the urinary metabolites were fractionated using HPLC, and GC-MS comparisons were again made for positive structural identification. The pathways for metabolite formation are not yet understood, but a mechanistic hypothesis has been proposed to account for the various metabolites observed thus far.
OGLE-2015-BLG-0479LA,B: BINARY GRAVITATIONAL MICROLENS CHARACTERIZED BY SIMULTANEOUS GROUND-BASED AND SPACE-BASED OBSERVATIONS

We present a combined analysis of the observations of the gravitational microlensing event OGLE-2015-BLG-0479 taken both from the ground and by the Spitzer Space Telescope. The light curves seen from the ground and from space exhibit a time offset of ∼13 days between the caustic spikes, indicating that the relative lens-source positions seen from the two places are displaced by parallax effects. From modeling the light curves, we measure the space-based microlens parallax. Combined with the angular Einstein radius measured by analyzing the caustic crossings, we determine the mass and distance of the lens. We find that the lens is a binary composed of two G-type stars with masses of ∼1.0 M⊙ and ∼0.9 M⊙ located at a distance of ∼3 kpc. In addition, we are able to constrain the complete orbital parameters of the lens thanks to the precise measurement of the microlens parallax derived from the joint analysis. In contrast to the binary event OGLE-2014-BLG-1050, which was also observed by Spitzer, we find that the interpretation of OGLE-2015-BLG-0479 does not suffer from the degeneracy between (±, ±) and (±, ∓) solutions, confirming that the four-fold parallax degeneracy in single-lens events collapses into the two-fold degeneracy for the general case of binary-lens events. The location of the blend in the color–magnitude diagram is consistent with the lens properties, suggesting that the blend is the lens itself. The blend is bright enough for spectroscopy and thus this possibility can be checked from future follow-up observations.
MiR-410 induces stemness by inhibiting Gsk3β but upregulating β-catenin in non-small cells lung cancer

Our previous research indicated miR-410 played a critical role in promoting the tumorigenesis and development of NSCLC (non-small cells lung cancer). MiR-410 has been recently reported to be crucial for development and differentiation of embryonic stem cells. But it remains elusive whether miR-410 stimulates the stemness of cancer until now. Herein, we identify miR-410 induces the stemness and is associated with the progression of NSCLC. We demonstrate miR-410 increases the levels of stem cells marker Sox2, Oct4, Nanog, CXCR4 as well as lung cancer stem cells surface marker CD44 and CD166. MiR-410 promotes stem cells-like properties such as proliferation, sphere formation, metastasis and chemoresistance. Moreover, Gsk3β is directly targeted and post-transcriptionally downregulated by miR-410. Also, the expression levels of miR-410 and Gsk3β may be correlated to clinicopathological differentiation in NSCLC tumor specimens. Additionally, we demonstrate miR-410 induces stemness through inhibiting Gsk3β but increasing Sox2, Oct4, Nanog and CXCR4, which binds to β-catenin signaling. In conclusion, our findings identify the miR-410/Gsk3β/β-catenin signaling axis is a novel molecular circuit in inducing stemness of NSCLC.