Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of intercellular adhesion molecule-1 (ICAM-1) with actin- containing cytoskeleton and alpha-actinin

We have studied the cytoskeletal association of intercellular adhesion molecule-1 (ICAM-1, CD54), an integral membrane protein that functions as a counterreceptor for leukocyte integrins (CD11/CD18). A linkage between ICAM-1 and cytoskeletal elements was suggested by studies showing a different ICAM-1 staining pattern for COS cells transfected with wild-type ICAM-1 or with an ICAM-1 construct that replaces the cytoplasmic and transmembrane domains of ICAM-1 with a glycophosphatidylinositol (GPI) anchor. Wild-type ICAM-1 appeared to localize most prominently in microvilli whereas GPI-ICAM-1 demonstrated a uniform cell surface distribution. Disruption of microfilaments with cytochalasin B (CCB) changed the localization of wild-type ICAM-1 but had no effect on GPI-ICAM-1. Some B-cell lines demonstrated a prominent accumulation of ICAM-1 into the uropod region whereas other cell surface proteins examined were not preferentially localized. CCB also induced redistribution of ICAM-1 in these cells. For characterization of cytoskeletal proteins interacting with ICAM-1, a 28-residue peptide that encompasses the entire predicted cytoplasmic domain (ICAM-1,478- 505) was synthesized, coupled to Sepharose-4B, and used as an affinity matrix. One of the most predominant proteins eluted either with soluble ICAM-1,478-505-peptide or EDTA, was 100 kD, had a pI of 5.5, and in Western blots reacted with alpha-actinin antibodies. A direct association between alpha-actinin and ICAM-1 was demonstrated by binding of purified alpha-actinin to ICAM-1,478-505-peptide and to immunoaffinity purified ICAM-1 and by a strict colocalization of ICAM-1 with alpha-actinin, but not with the cytoskeletal proteins talin, tensin, and vinculin. The region of ICAM-1,478-505 interacting with alpha-actinin was mapped to the area close to the membrane spanning region. This region contains several positively charged residues and appears to mediate a charged interaction with alpha-actinin which is not highly dependent on the order of the residues.
The Smart City is landing! On the geography of policy mobility

Abstract The aim of this paper is to provide a geographical urban policy perspective on the strategy of Smart Cities in the specific context of Czechia. Considering that the implementation of the Smart Cities (SC) concept is still relatively young in Czechia, it is highly relevant to examine the time-space diffusion of this concept in Czechia in the geographical lens: where the first initiative to build a smart city started, when the process was started and by whom; in other words, to provide basic empirical evidence of understanding the policy mobility and implementation of smart city policy into the urban development strategies. In the first of our approach, we evaluate the implementation of the term “smart city” in strategic city documents. The next step is the analysis of the strategic urban (city) and smart city documents by distinguishing conceptually distinct pillars of the SC concept and an overview of actors and policy-makers who initiate and support individual pillars of the concept of SC in Czechia. The results of the analysis highlight the differences between the implemented SC topics into city strategies which are caused by fragmented policy mobility, its modifications and influence of key actors who have found the opportunity to participate in policy-making processes at the certain spatial level.
Network Traffic Classification techniques and comparative analysis using Machine Learning algorithms

Network Traffic Classification is a central topic nowadays in the field of computer science. It is a very essential task for internet service providers (ISPs) to know which types of network applications flow in a network. Network Traffic Classification is the first step to analyze and identify different types of applications flowing in a network. Through this technique, internet service providers or network operators can manage the overall performance of a network. There are many methods traditional technique to classify internet traffic like Port Based, Pay Load Based and Machine Learning Based technique. The most common technique used these days is Machine Learning (ML) technique. Which is used by many researchers and got very effective accuracy results. In this paper, we discuss network traffic classification techniques step by step and real time internet data set is develop using network traffic capture tool, after that feature extraction tool is use to extract features from the capture traffic and then four machine learning classifiers Support Vector Machine, C4.5 decision tree, Naïve Bays and Bayes Net classifiers are applied. Experimental analysis shows that C4.5 classifiers gives very good accuracy result as compare to other classifies.
Cinnamon: A Multifaceted Medicinal Plant

Cinnamon (Cinnamomum zeylanicum, and Cinnamon cassia), the eternal tree of tropical medicine, belongs to the Lauraceae family. Cinnamon is one of the most important spices used daily by people all over the world. Cinnamon primarily contains vital oils and other derivatives, such as cinnamaldehyde, cinnamic acid, and cinnamate. In addition to being an antioxidant, anti-inflammatory, antidiabetic, antimicrobial, anticancer, lipid-lowering, and cardiovascular-disease-lowering compound, cinnamon has also been reported to have activities against neurological disorders, such as Parkinson's and Alzheimer's diseases. This review illustrates the pharmacological prospective of cinnamon and its use in daily life.
Reactive Iron and Iron‐Bound Organic Carbon in Surface Sediments of the River‐Dominated Bohai Sea (China) Versus the Southern Yellow Sea

Factors influencing reactive Fe cycling and its protection of organic carbon (OC) in sediments are poorly understood. Here we comparatively study Fe speciation and Fe‐associated OC (Fe‐OC) in surface sediments of the Bohai Sea (BHS) and southern Yellow Sea (SYS), two seas with common sediment sources but different depositional regimes. Though significant sequestration of highly reactive Fe (FeHR) is expected in the estuarine system stream from the river‐dominated BHS, this pool is, however, slightly enriched in the BHS sediments relative to their source material. This reconfirms a previous speculation of sedimentary FeHR enrichment in semi‐protected settings. Relative to the BHS, the SYS sediments are depleted of FeHR, despite common sediment sources of these two areas. Estuarine pre‐enrichment and subsequent redistribution of FeHR in the BHS, and aging of Fe‐bearing authigenic clays during transport from the BHS to the SYS are potential mechanisms for the depletion. The fractions (fFe‐OC) of Fe‐OC in total OC in sediments of the two seas are at the lower end for soils and sediments, indicating Fe being a minor “rusty sink. ” 13CFe‐OC fractionations indicate preferential sequestration of terrestrial OC by Fe oxides in the BHS, in contrast with preferential retention of marine OC in the SYS. Different fractionations of 13CFe‐OC in the two seas are a net result of selective adsorption of OC by Fe oxides and selective stabilization of OC during Fe reductive dissolution. Preferential sequestration of terrestrial OC may exert an important influence on distribution and compositions of OC buried in the river‐dominated system.
A comparative study of small-signal average models for average current mode control

This paper presents a critical evaluation of four small-signal average models for average current mode control. The four models are divided into two groups. One group includes sampling effect in the model formulation that has been used successfully for peak current mode control, and the other does not include it. The models are evaluated against experimental data generated by using a Buck converter as a common test bed under various converter circuit and operating parameter changes. For practical design purposes, results are interpreted and explained by employing a simplified version of the second group of models to reveal more insight into average current model control. The study finds similarities and differences among these models as well as their limitations in predicting small-signal dynamics of average-current-mode-controlled DC-DC converters.
Safety and immunologic effects of high- vs low-dose cholecalciferol in multiple sclerosis

Editors' Note: Commenting on “Pearls & Oy-sters: Tacrolimus neurotoxicity presenting as an isolated brainstem lesion,” Dr. Chang wonders whether the patient reported had, in fact, posterior reversible encephalopathy syndrome (PRES) rather than a direct neurotoxic effect of tacrolimus. Drs. Saadi and Schmahmann, authors of the article, explain that the patient's blood pressure was within the normal range throughout hospitalization, making PRES unlikely. Critiquing “Safety and immunologic effects of high- vs low-dose cholecalciferol in multiple sclerosis,” Dr. Kantor comments that the Class I evidence of safety/tolerability is incorrect because the trial was not powered to demonstrate such an effect, and because of differences in urine calcium:creatinine ratio, incidence of nausea, and hypercalcemia between the 2 groups. Sotirchos et al., authors of the article, disagree with these …
Retiming sequential circuits for low power

Switching activity is the primary cause of power dissipation in CMOS combinational and sequential circuits. We give a method of estimating power in pipelined sequential CMOS circuits that accurately models the correlation between the vectors applied to the combinational logic of the circuit. We explore the implications of the observation that the switching activity at flip-flop outputs in a synchronous sequential circuit can be significantly less than the activity at the flip-flop inputs. We present a retiming method that targets the power dissipation of a sequential circuit.
C455R notch3 mutation in a Colombian CADASIL kindred with early onset of stroke

Abstract—Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is caused by mutations in the notch3 epidermal growth factor–like repeats. A Colombian kindred carries a novel C455R mutation located in the predicted ligand-binding domain. Stroke occurred in the patients at an unusually early age (median age: 31 years) in comparison to the more frequent onset in the fourth decade of life in other CADASIL populations, including a second Colombian kindred with an R1031C mutation.
Internalism, Active Externalism, and Nonconceptual Content: The Ins and Outs of Cognition

Active externalism (also known as the extended mind hypothesis) says that we use objects and situations in the world as external memory stores that we consult as needs dictate. This gives us economies of storage: We do not need to remember that Bill has blue eyes and wavy hair if we can acquire this information by looking at Bill. I argue for a corollary to this position, which I call 'internalism. ' Internalism says we can acquire knowledge on a need-to-know basis by consulting portable, inner analogues of the world. This, however, leads to a dilemma. If the knowledge was stored in memory, it is difficult to see how we could have acquired it by consulting inner analogues, for it would seem that we knew it already. Moreover, if it was stored in memory, we lose the economies of storage that provide much of the rationale for external memory stores and hence for their inner analogues. If, on the other hand, the knowledge was not stored in memory, it is difficult to see how we could have acquired it by consulting inner analogues, for it was not there to be acquired. I propose a solution to this problem that turns on the concept of nonconceptual content, and I relate the solution to Stephen Kosslyn's (1994) architecture of perception and visual mental imagery. Viewed in a broader context, the solution shows that the world leaks into the mind, as well as mind leaking into the world.
Pacific Ocean Heat Content During the Past 10,000 Years

, 617 (2013); 342 Science et al. Yair Rosenthal Pacific Ocean Heat Content During the Past 10,000 Years This copy is for your personal, non-commercial use only. clicking here. colleagues, clients, or customers by , you can order high-quality copies for your If you wish to distribute this article to others here. following the guidelines can be obtained by Permission to republish or repurpose articles or portions of articles ): November 4, 2013 www.sciencemag. org (this information is current as of The following resources related to this article are available online at http://www.sciencemag. org/content/342/6158/617.full. html version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag. org/content/suppl/2013/10/30/342.6158. 617.DC1. html can be found at: Supporting Online Material http://www.sciencemag. org/content/342/6158/617.full. html#ref-list-1 , 4 of which can be accessed free: cites 29 articles This article
Overuse of organ biopsies in immunoglobulin light chain amyloidosis (AL): the consequence of failure of early recognition

Abstract Introduction: The diagnosis of amyloidosis requires histological confirmation of Congo-red (CR) deposits. The tissue source is preferably fat aspiration and/or bone marrow (BM) biopsy, but at times organ biopsy is required. Methods: We studied 612 patients with systemic immunoglobulin light chain amyloidosis to characterise the tissues used to establish the diagnosis. Results: The median number of tissue samples was 3. About 95% of BM biopsies were stained for CR, while 79% of patients had fat aspiration CR-stained. CR stain sensitivity was 69% in BM, 75% in fat aspiration and 89% for both sources combined. In comparison, CR sensitivity was 97–100% for heart, renal and liver biopsies. About 42% of patients with renal involvement, 21% of patients with liver involvement and 13% of patients with heart involvement underwent organ biopsy, when a less invasive biopsy would have established the diagnosis. Predictors for the requirement for organ biopsy were male sex, limited organ involvement and lack of fat aspiration. Discussion: Fat aspiration is underutilised for histologic confirmation of amyloidosis. A high rate of organ biopsies represents a failure to recognise the disease. Early awareness of amyloidosis in patients with organ dysfunction may lead to more judicious use of organ biopsies in this disease. Key messages Fat pad aspiration is underutilised to establish the diagnosis of amyloidosis. Bone marrow and fat pad aspiration obviates the need for invasive biopsies. The excessive use of organ biopsy in AL amyloidosis reflects failure to recognise the disease early in its course.
Genetically modified pig models for neurodegenerative disorders

Increasing incidence of neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease has become one of the most challenging health issues in ageing humans. One approach to combat this is to generate genetically modified animal models of neurodegenerative disorders for studying pathogenesis, prognosis, diagnosis, treatment, and prevention. Owing to the genetic, anatomic, physiologic, pathologic, and neurologic similarities between pigs and humans, genetically modified pig models of neurodegenerative disorders have been attractive large animal models to bridge the gap of preclinical investigations between rodents and humans. In this review, we provide a neuroanatomical overview in pigs and summarize and discuss the generation of genetically modified pig models of neurodegenerative disorders including Alzheimer's diseases, Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, spinal muscular atrophy, and ataxia–telangiectasia. We also highlight how non‐invasive bioimaging technologies such as positron emission tomography (PET), computer tomography (CT), and magnetic resonance imaging (MRI), and behavioural testing have been applied to characterize neurodegenerative pig models. We further propose a multiplex genome editing and preterm recloning (MAP) approach by using the rapid growth of the ground‐breaking precision genome editing technology CRISPR/Cas9 and somatic cell nuclear transfer (SCNT). With this approach, we hope to shorten the temporal requirement in generating multiple transgenic pigs, increase the survival rate of founder pigs, and generate genetically modified pigs that will more closely resemble the disease‐causing mutations and recapitulate pathological features of human conditions. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Effect of Lipid-Hydroperoxide-Induced Oxidative Stress on Vitamin E, Ascorbate and Glutathione in the Rabbit Retina

Background: It is possible that oxidative stress causes several retinal diseases. However, the natural biogenic role of antioxidants in the retina is not clear. Purpose: This study investigates the change in concentration of vitamin E (VE), ascorbate and glutathione (GSH) in the retina following vitreous injection of 600 µg 18:2 linoleic acid hydroperoxide (LHP) in male New Zealand rabbits. Method: LHP was injected above the retinal surface. The animals were sacrificed and the eyes enucleated before LHP injection, 1, 3, 6, 12, 24 h and 4 and 7 days after LHP injection. Retinas were removed, VE and ascorbate measured by HPLC, and GSH determined by a fluorometric method. Results: The concentration of VE in the retina decreased from pretreatment levels of 154.6 ± 29.7 nmol/g wet weight (n = 7) and was lowest at 6 h (61.1 ± 18.1 nmol/g wet weight, n = 4, p < 0.05), then increased gradually, returning slowly to pre-LHP levels by 7 days. The concentration of ascorbate in control retinas decreased at 6 h from pretreatment levels of 7.33 ± 0.93 µmol/g wet weight (n = 7) to 2.74 ± 0.16 µmol/g wet weight (n = 4, p < 0.05) and returned to pretreatment levels rapidly by 24 h after injection. The concentration of GSH in retinas decreased from baseline levels of 109.53 ± 8.19 µg/g wet weight (n = 9), was lowest at 12 h (72.40 ± 11.17 µg/g wet weight, n = 5, p < 0.05) and returned to pretreatment levels by 7 days. Conclusion: The results suggest that intravitreous LHP injection is a contributor to oxidative stress in the rabbit retina by causing a reduction in antioxidant capacity.
Cross-Modal Transfer of Information between the Tactile and the Visual Representations in the Human Brain: A Positron Emission Tomographic Study

Positron emission tomography in three-dimensional acquisition mode was used to identify the neural populations involved in tactile–visual cross-modal transfer of shape. Eight young male volunteers went through three runs of three different matching conditions: tactile–tactile (TT), tactile–visual (TV), and visual–visual (VV), and a motor control condition. Fifteen spherical ellipsoids were used as stimuli. By subtracting the different matching conditions and calculating the intersections of statistically significant activations, we could identify cortical functional fields involved in the formation of visual and tactile representation of the objects alone and those involved in cross-modal transfer of the shapes of the objects. Fields engaged in representation of visual shape, revealed in VV–control, TV–control and TV–TT, were found bilaterally in the lingual, fusiform, and middle occipital gyri and the cuneus. Fields engaged in the formation of the tactile representation of shape, appearing in TT–control, TV–control and TV–VV, were found in the left postcentral gyrus, left superior parietal lobule, and right cerebellum. Finally, fields active in both TV–VV and TV–TT were considered as those involved in cross-modal transfer of information. One field was found, situated in the right insula–claustrum. This region has been shown to be activated in other studies involving cross-modal transfer of information. The claustrum may play an important role in cross-modal matching, because it receives and gives rise to multimodal cortical projections. We propose here that modality-specific areas can communicate, exchange information, and interact via the claustrum.
Pattern Trees Induction: A New Machine Learning Method

Fuzzy classification is one of the most important applications in fuzzy set and fuzzy-logic-related research. Its goal is to find a set of fuzzy rules that form a classification model. Most of the existing fuzzy rule induction methods (e.g., the fuzzy decision trees (FDTs) induction method) focus on searching rules consisting of triangular norms (t-norms) (i.e., and) only, but not triangular conorms (t-conorms) (or) explicitly. This may lead to the omission of generating important rules that involve t-conorms explicitly. This paper proposes a type of tree termed pattern trees (PTs) that makes use of different aggregations, including both t-norms and t-conorms. Like decision trees, PTs are an effective tool for classification applications. This paper discusses the difference between decision trees and PTs, and also shows that the subsethood-based method (SBM) and the weighted-subsethood-based method (WSBM) are two specific cases of PT induction. A novel PT induction method is proposed using similarity measure and fuzzy aggregations. The comparison to other classification methods including SBM, WSBM, C4.5, nearest neighbor, support vector machine, and FDT induction shows that: 1) PTs can obtain high accuracy rates in classifications; 2) PTs are robust to overfltting; and 3) PTs, especially simple pattern trees (SPTs), maintain compact tree structures.
Can Dynamic Contrast-Enhanced Magnetic Resonance Imaging Combined with Texture Analysis Differentiate Malignant Glioneuronal Tumors from Other Glioblastoma?

An interesting approach has been proposed to differentiate malignant glioneuronal tumors (MGNTs) as a subclass of the WHO grade III and IV malignant gliomas. MGNT histologically resemble any WHO grade III or IV glioma but have a different biological behavior, presenting a survival twice longer as WHO glioblastomas and a lower occurrence of metastases. However, neurofilament protein immunostaining was required for identification of MGNT. Using two complementary methods, dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and texture analysis (MRI-TA) from the same acquisition process, the challenge is to in vivo identify MGNT and demonstrate that MRI postprocessing could contribute to a better typing and grading of glioblastoma. Results are obtained on a preliminary group of 19 patients a posteriori selected for a blind investigation of DCE T1-weighted and TA at 1.5 T. The optimal classification (0/11 misclassified MGNT) is obtained by combining the two methods, DCE-MRI and MRI-TA.
The Role of Canalicular ABC Transporters in Cholestasis

Cholestasis, a hallmark feature of hepatobiliary disease, is characterized by the retention of biliary constituents. Some of these constituents, such as bile acids, inflict damage to hepatocytes and bile duct cells. This damage may lead to inflammation, fibrosis, cirrhosis, and eventually carcinogenesis, sequelae that aggravate the underlying disease and deteriorate clinical outcome. Canalicular ATP-binding cassette (ABC) transporters, which mediate the excretion of individual bile constituents, play a key role in bile formation and cholestasis. The study of these transporters and their regulatory nuclear receptors has revolutionized our understanding of cholestatic disease. This knowledge has served as a template to develop novel treatment strategies, some of which are currently already undergoing phase III clinical trials. In this review we aim to provide an overview of the structure, function, and regulation of canalicular ABC transporters. In addition, we will focus on the role of these transporters in the pathogenesis and treatment of cholestatic bile duct and liver diseases.