Miracle-dz/UniFAR-FTUs huggingface dataset:
T Cells Lacking Immunoproteasome Subunits MECL-1 and LMP7 Hyperproliferate in Response to Polyclonal Mitogens1

Immunoproteasomes comprise a specialized subset of proteasomes that is defined by the presence of three catalytic immunosubunits: LMP2, MECL-1 (LMP10), and LMP7. Proteasomes in general serve many cellular functions through protein degradation, whereas the specific function of immunoproteasomes has been thought to be largely, if not exclusively, optimization of MHC class I Ag processing. In this report, we demonstrate that T cells from double knockout mice lacking two of the immunosubunits, MECL-1 and LMP7, hyperproliferate in vitro in response to various polyclonal mitogens. We observe hyperproliferation of both CD4+ and CD8+ T cell subsets and demonstrate accelerated cell cycling. We do not observe hyperproliferation of T cells lacking only one of these subunits, and thus hyperproliferation is independent of either reduced MHC class I expression in LMP7−/− mice or reduced CD8+ T cell numbers in MECL-1−/− mice. We observe both of these latter two phenotypes in MECL-1/LMP7−/− mice, which indicates that they also are independent of each other. Finally, we provide evidence of in vivo T cell dysfunction by demonstrating increased numbers of central memory phenotype CD8+ T cells in MECL-1/LMP7−/− mice. In summary, this novel phenotype of hyperproliferation of T cells lacking both MECL-1 and LMP7 implicates a specific role for immunoproteasomes in T cell proliferation that is not obviously connected to MHC class I Ag processing.
Control of plants which change using switching controllers

A recent problem in adaptive control has been the task of controlling a system that cannot be characterized by a single mathematical model, and instead undergoes changes within a certain family of models. One strategy for controlling such a system has been to use switching between a fixed number of controllers to compensate for changing plant dynamics. This paper considers the problem of controlling an unknown plant contained in a family of models together with the additional task of controlling a plant whose plant dynamics shift from one member of the family to another. In particular, this paper describes a new switching controller that uses observers to improve switching transients, while maintaining an acceptable level of robustness.
Dielectric Relaxation of Biological Water

Dielectric relaxation and NMR spectrum of water in biological systems such as proteins, DNA, and reverse micelles can often be described by two widely different time constants, one of which is in the picosecond while the other is in the nanosecond regime. Although it is widely believed that the bimodal relaxation arises from water at the hydration shell, a quantitative understanding of this important phenomenon is lacking. In this article we present a theory of dielectric relaxation of biological water. The time dependent relaxation of biological water is described in terms of a dynamic equilibrium between the free and bound water molecules. It is assumed that only the free water molecules undergo orientational motion; the bound water contribution enters only through the rotation of the biomolecule, which is also considered. The dielectric relaxation is then determined by the equilibrium constant between the two species and the rate of conversion from bound to free state and vice versa. However, the diele.
Cancellation of Ventricular Activity in the ECG: Evaluation of Novel and Existing Methods

Due to the much higher amplitude of the electrical activity of the ventricles in the surface electrocardiogram (ECG), its cancellation is crucial for the analysis and characterization of atrial fibrillation. In this paper, two different methods are proposed for this cancellation. The first one is an average beat subtraction type of method. Two sets of templates are created: one set for the ventricular depolarization waves and one for the ventricular repolarization waves. Next, spatial optimization (rotation and amplitude scaling) is applied to the QRS templates. The second method is a single beat method that cancels the ventricular involvement in each cardiac cycle in an independent manner. The estimation and cancellation of the ventricular repolarization is based on the concept of dominant T and U waves. Subsequently, the atrial activities during the ventricular depolarization intervals are estimated by a weighted sum of sinusoids observed in the cleaned up segments. ECG signals generated by a biophysical model as well as clinical ECG signals are used to evaluate the performance of the proposed methods in comparison to two standard ABS-based methods
Design of an Efficient Pumping Scheme for Mid-IR Dy3+:Ga5Ge20Sb10S65 PCF Fiber Laser

This letter illustrates the design of a novel medium infrared (Mid-IR) laser based on a photonic crystal fiber made of dysprosium-doped chalcogenide glass, Dy3+:Ga5Ge20Sb10S65. In order to perform a realistic investigation, the simulation is performed by taking into account the spectroscopic parameters measured on the rare earth-doped glass sample. The simulated results show that an optical beam emission close to 4400-nm wavelength can be obtained by employing two pump beams at 2850 nm (pump #1) and 4092 nm (pump #2) wavelengths. The pump beams can be provided by commercial quantum cascade lasers. As example, for the pump powers of 50 mW (pump #1) and 1 W (pump #2), the input mirror reflectivity of 99%, the output mirror reflectivity of 30%, and the optical cavity length of 50 cm, a signal power close to 350 mW at the wavelength of 4384 nm can be generated. This result indicates that the designed source configuration is feasible for high beam quality Mid-IR light generation and it is efficient enough to find applications in optical free propagation links, optical remote sensing, and medicine.
Molecular targets for ameliorating early brain injury post subarachnoid hemorrhage: a new focus.

of magnitude lower than presently accepted values. Am J Physiol Regul Integr Comp Physiol 2008; 295:R1479–R1485 6. Yang G, Wu L, Jiang B, et al: H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gammalyase. Science 2008; 322:587–590 7. Yang G, Wu L, Wang R: Pro-apoptotic effect of endogenous H2S on human aorta smooth muscle cells. FASEB J 2006; 20:553–555 8. Zanardo RC, Brancaleone V, Distrutti E, et al: Hydrogen sulfide is an endogenous modulator of leukocyte-mediated inflammation. FASEB J 2006; 20:2118–2120 9. Yang G, Cao K, Wu L, et al: Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/ WAK-1. J Biol Chem 2004; 279:49199–49205 10. Szabo C: Hydrogen sulphide and its therapeutic potential. Nat Rev Drug Discov 2007; 6:917–935 11. Wagner F, Asfar P, Calzia E, et al: Bench-tobedside review: Hydrogen sulfide—The third gaseous transmitter: Applications for critical care. Crit Care 2009; 13:213 12. Blackstone E, Morrison M, Roth MB: H2S induces a suspended animation-like state in mice. Science 2005; 308:518 13. Sodha NR, Clements RT, Feng J, et al: Hydrogen sulfide therapy attenuates the inflammatory response in a porcine model of myocardial ischemia/reperfusion injury. J Thorac Cardiovasc Surg 2009; 138:977–984 14. Minamishima S, Bougaki M, Sips PY, et al: Hydrogen sulfide improves survival after cardiac arrest and cardiopulmonary resuscitation via a nitric oxide synthase 3-dependent mechanism in mice. Circulation 2009; 120: 888–896 15. Morrison ML, Blackwood JE, Lockett SL, et al: Surviving blood loss using hydrogen sulfide. J Trauma 2008; 65:183–188 16. Blackstone E, Roth MB: Suspended animation-like state protects mice from lethal hypoxia. Shock 2007; 27:370–372 17. Baumgart K, Wagner F, Groger M, et al: Cardiac and metabolic effects of hypothermia and inhaled hydrogen sulfide in anesthetized and ventilated mice. Crit Care Med 2010; 38:588–595 18. Leverve XM: Mitochondrial function and substrate availability. Crit Care Med 2007; 35:S454–S460
Measuring the Multimedia Performance of Server-Based Computing

The server-based computing (SBC) model is becoming an increasingly popular approach for delivering computational services with reduced administrative costs and better resource utilization. In this paper, we examine how effectively SBC architectures support multimedia applications. We focus on the effectiveness of the remote display protocol used in three popular SBC platforms for supporting video applications, Citrix Metaframe, Windows Terminal Server, and AT&T VNC. Our results show that SBC can be a viable approach for delivering VCR-quality video in LAN environments, but that existing solutions are inadequate at network access bandwidths found in broadband environments. Our results also show that SBC can deliver video with comparable network efficiency as streaming media solutions. We show that there is wide variation in the performance of the remote display technologies and discuss the factors that influence their performance.
Positioning in Media Dialogue: Negotiating roles in the news interview

This book proposes a socio-pragmatic exploration of the discursive practices used to construe and dynamically negotiate positions in news interviews. It starts with a discursive interpretation of ‘positioning’, ‘role’ and ‘challenge’, puts forward the relevance of a distinction between social and interactional roles, demonstrates how challenges bring to the fore the relevant roles and role-components of the participants, and shows that in news interviews speakers constantly position and re-position themselves and each other through discourse.The discussion draws on an empirical fine-grained analysis of a 24-hour corpus of news interviews on Israeli television and a corpus of media references. The author postulates a discrepancy between interlocutors’ normative expectations, which presuppose an asymmetrical division of labor, on the one hand, and real-life practice, which exhibits partial symmetry in speakers’ selection of discourse patterns as well as reciprocity in the use of challenge strategies, on the other. Special attention is given to irony and terms of address, which are shown to act as the center-points of satellite challenge strategies, geared as an ensemble toward the co-construction of reciprocal positioning. The analysis of three case studies further sheds light on the negotiations of intertwined positionings in context.
HSV-2-Driven Increase in the Expression of α4β7 Correlates with Increased Susceptibility to Vaginal SHIVSF162P3 Infection

The availability of highly susceptible HIV target cells that can rapidly reach the mucosal lymphoid tissues may increase the chances of an otherwise rare transmission event to occur. Expression of α4β7 is required for trafficking of immune cells to gut inductive sites where HIV can expand and it is expressed at high level on cells particularly susceptible to HIV infection. We hypothesized that HSV-2 modulates the expression of α4β7 and other homing receptors in the vaginal tissue and that this correlates with the increased risk of HIV acquisition in HSV-2 positive individuals. To test this hypothesis we used an in vivo rhesus macaque (RM) model of HSV-2 vaginal infection and a new ex vivo model of macaque vaginal explants. In vivo we found that HSV-2 latently infected RMs appeared to be more susceptible to vaginal SHIVSF162P3 infection, had higher frequency of α4β7 high CD4+ T cells in the vaginal tissue and higher expression of α4β7 and CD11c on vaginal DCs. Similarly, ex vivo HSV-2 infection increased the susceptibility of the vaginal tissue to SHIVSF162P3. HSV-2 infection increased the frequencies of α4β7 high CD4+ T cells and this directly correlated with HSV-2 replication. A higher amount of inflammatory cytokines in vaginal fluids of the HSV-2 infected animals was similar to those found in the supernatants of the infected explants. Remarkably, the HSV-2-driven increase in the frequency of α4β7 high CD4+ T cells directly correlated with SHIV replication in the HSV-2 infected tissues. Our results suggest that the HSV-2-driven increase in availability of CD4+ T cells and DCs that express high levels of α4β7 is associated with the increase in susceptibility to SHIV due to HSV-2. This may persists in absence of HSV-2 shedding. Hence, higher availability of α4β7 positive HIV target cells in the vaginal tissue may constitute a risk factor for HIV transmission.
Obligation-Based Agent Conversations for Semantic Web Service Composition

We deal with composition of semantic web services to which access is controlled by agents. We propose a conversation-based web service composition method. We conceive web services as actions with preconditions and effects, expressed in terms of social norms, particularly obligations. We argue that the inclusion of obligation-based agents' conversations aide to lead the composition of services. In order to achieve this, we introduce an agent communication language that defines how messages affect agents' state, and thus, the access to their services. We also propose a method to automatically create a generic composer agent that is able to manage and compose web services, by means of inducing obligations to their participants.
Self-rated health of Chinese adolescents: distribution and its associated factors.

AIM Self-rated health is a valid and relevant indicator of individuals' and populations' health status, and has been shown to be a critical predictor of some medical endpoints. The studies concentrating on Chinese adolescents' self-rated health were scarce. The present study aimed to understand the self-rated health and its associated factors of Chinese adolescents. METHODS Ten schools including junior schools, senior schools and universities of Hefei in Anhui Province of China were randomly selected. A cross-sectional survey was conducted to the selected 5226 students to investigate their demographic variables, sleep quality, self-rated health and degree of depression, anxiety and loneliness. In terms of the instruments, Self-rated Health Measurement Scale Version 1.0, Pittsburgh Sleep Quality Index, UCLA Loneliness Scale, Beck Depression Inventory, Self-rating Anxiety Scale and the demographic survey were used in the present study. RESULTS The sleep quality, self-rated health and degree of depression, anxiety and loneliness among different grade levels were different (p < 0.01 or <0.05). The multivariate analysis indicated that young age, poor sleep quality and high degree of depression, anxiety and loneliness were significantly associated with poor self-rated health of adolescents. CONCLUSION The adolescents' poor self-rated health may be attributed to many causes especially the psychological reasons. Future interventions that focus more on the inner causes may be the better steps to make adolescents healthy.
UFM1 Protects Macrophages from oxLDL-Induced Foam Cell Formation Through a Liver X Receptor α Dependent Pathway.

AIM Macrophage foam cell formation is the most prominent characteristic of the early stages of atherosclerosis. Ubiquitin Fold Modifier 1 (UFM1) is a new member of the ubiquitin-like protein family, and its underlying mechanism of action in macrophage foam cell formation is poorly understood. Our current study focuses on UFM1 and investigates its role in macrophage foam cell formation. METHODS Using real-time quantitative PCR (qRT-PCR) and western blot analysis, we first analyzed the UFM1 expression in mouse peritoneal macrophages (MPMs) from ApoE－/－ mice in vivo and in human macrophages treated with oxLDL in vitro. Subsequently, the effects of UFM1 on macrophages foam cell formation were determined by Nile Red staining and direct lipid analysis. We then examined whether UFM1 affects the process of lipid metabolism in macrophages. Lastly, with the method of small interfering RNA (siRNA), we delineated the mechanism of UFM1 to attenuate lipid accumulation in THP-1 macrophages. RESULTS UFM1 is dramatically upregulated under atherosclerosis conditions both in vivo and in vitro. Moreover, UFM1 markedly decreased macrophage foam cell formation. Mechanistic studies revealed that UFM1 increased the macrophage cholesterol efflux, which was due to the increased expression of ATP-binding cassette transporters A1 (ABCA1) and G1 (ABCG1). Furthermore, the upregulation of ABCA1 and ABCG1 by UFM1 resulted from liver X receptor α (LXRα) activation, which was confirmed by the observation that LXRα siRNA prevented the expression of ABCA1 and ABCG1. Consistent with this, the UFM1-mediated attenuation of lipid accumulation was abolished by such inhibition. CONCLUSIONS Taken together, our results showed that UFM1 could suppress foam cell formation via the LXRα-dependent pathway.
Cross-Trees for Stereo Matching with Priors

We propose a cross-trees structure to perform the non-local cost aggregation for dense stereo matching. The cross-trees structure consists of a horizontal-tree and a vertical-tree. Compared to other spanning trees, the significant superiority of the cross-trees is that the trees' constructions are efficient and independent on any local or global property. Moreover, the trees are exactly unique. By traversing the two crossed trees successively, a fast non-local cost aggregation algorithm is performed to filter the matching cost volume and then the disparity maps are established with the Winner-Take-All (WTA) strategy. Additionally, two different priors: edge prior and super pixel prior, are proposed to tackle the false smoothing at the depth boundaries. Hence, our method contains two different algorithms in terms of the cross-trees prior in this paper. Performance evaluation on the 27 Middlebury data sets shows that both our algorithms outperform the other two tree-based methods, namely minimum spanning tree (MST) and segment-tree (ST). By performing the non-local cost aggregation on different trees, MST, ST and our method all have competitive rankings on the Middlebury website compared to the local cost aggregation methods.
AFRICAN TRYPANOSOME INTERACTIONS WITH AN IN VITRO MODEL OF THE HUMAN BLOOD–BRAIN BARRIER

The neurological manifestations of sleeping sickness in man are attributed to the penetration of the blood–brain barrier (BBB) and invasion of the central nervous system by Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense. However, how African trypanosomes cross the BBB remains an unresolved issue. We have examined the traversal of African trypanosomes across the human BBB using an in vitro BBB model system constructed of human brain microvascular endothelial cells (BMECs) grown on Costar Transwell™ inserts. Human-infective T. b. gambiense strain IL 1852 was found to cross human BMECs far more readily than the animal-infective Trypanosoma brucei brucei strains 427 and TREU 927. Tsetse fly–infective procyclic trypomastigotes did not cross the human BMECs either alone or when coincubated with bloodstream-form T. b. gambiense. After overnight incubation, the integrity of the human BMEC monolayer measured by transendothelial electrical resistance was maintained on the inserts relative to the controls when the endothelial cells were incubated with T. b. brucei. However, decreases in electrical resistance were observed when the BMEC-coated inserts were incubated with T. b. gambiense. Light and electron microscopy studies revealed that T. b. gambiense initially bind at or near intercellular junctions before crossing the BBB paracellularly. This is the first demonstration of paracellular traversal of African trypanosomes across the BBB. Further studies are required to determine the mechanism of BBB traversal by these parasites at the cellular and molecular level.
Effects of Hepatic Drug-metabolizing Enzyme Induction on Clinical Pathology Parameters in Animals and Man

Hepatic drug-metabolizing enzyme (DME) induction is an adaptive response associated with changes in preclinical species; this response can include increases in liver weight, hepatocellular hyperplasia and hypertrophy, and upregulated tissue expression of DMEs. Effects of DME induction on clinical pathology markers of hepatobiliary injury and function in animals as well as humans are not well established. This component of a multipart review of the comparative pathology of xenobiotically mediated induction of hepatic metabolizing enzymes reviews pertinent data from retrospective and prospective preclinical and clinical studies. Particular attention is given to studies with confirmation of DME induction and concurrent evaluation of liver and/or serum hepatobiliary marker enzyme activities and histopathology. These results collectively indicate that in the rat, when histologic findings are limited to hepatocellular hypertrophy, DME induction is not expected to be associated with consistent or substantive changes in serum or plasma activity of hepatobiliary marker enzymes such as alanine aminotransferase, alkaline phosphatase, and gamma glutamyltransferase. In the dog and the monkey, published studies also do not demonstrate a consistent relationship across DME-inducing agents and changes in these clinical pathology parameters. However, increased liver alkaline phosphatase or gamma glutamyltransferase activity in dogs treated with phenobarbital or corticosteroids suggests that direct or indirect induction of select hepatobiliary injury markers can occur both in the absence of liver injury and independently of induction of DME activity. Although correlations between tissue and serum levels of these hepatobiliary markers are limited and inconsistent, increases in serum/plasma activities that are substantial or involve changes in other markers generally reflect hepatobiliary insult rather than DME induction. Extrahepatic effects, including disruption of the hypothalamic-pituitary-thyroid axis, can also occur as a direct outcome of hepatic DME induction in humans and animals. Importantly, hepatic DME induction and associated changes in preclinical species are not necessarily predictive of the occurrence, magnitude, or enzyme induction profile in humans.
William J. Gedney’s Comparative Tai Source Book

This volume provides accurate and reliable data from 1,159 common cognates found in 19 dialects from the Tai language family. Originally collected by noted Tai linguist the late William J. Gedney, the data are organized into the three branches of the Tai language family, the Southwestern, the Central, and the Northern, to facilitate comparisons among the various sound systems within the individual branches and within the Tai language family as a whole. Supplementing the cognates are phonological descriptions of each of the dialects.Included among the nineteen dialects are Siamese, White Tai, Black Tai, Shan, Lue, Yay, Saek, and dialects found at Leiping, Lungming, Pingsiang, and Ningming in China. The meticulous attention paid to consonants, vowels, and tones found in each cognate will allow for further dialect studies, for the investigation of questions concerning the tripartite division of the Tai language family, and for the continuing investigation into the reconstruction of the Proto-Tai language family and its wider genetic relationships.