Miracle-dz/UniFAR-FTUs huggingface dataset:
Electrophysiologic Characterization of Dronedarone in Guinea Pig Ventricular Cells

The electrophysiological properties of dronedarone (SR33589), a noniodinated amiodarone-like agent, were studied on action potential (AP) and contraction of papillary muscle and on membrane ionic currents, Ca2+ transient, and shortening of ventricular cells of the guinea pig heart. In multicellular preparations, dronedarone (3, 10, and 30 &mgr;M) decreased maximum rate of rise of AP (dV/dt max) with a concentration- and frequency-dependent relationship; resting potential was not modified and AP amplitude was decreased only at 30 &mgr;M. The effects of dronedarone on AP durations (APDs) at different percentages of repolarization were not significantly changed, except for a slight decrease in APD30 and APD50 at the highest concentration. In isolated ventricular myocytes, dronedarone inhibited rapidly activating delayed-rectifier K+ current (IKr) (median inhibitory concentration [IC50] ≤ 3 &mgr;M; voltage-independent); slowly activating delayed-rectifier K+ current (IKs) (IC50EQUATION 10 &mgr;M; voltage-dependent and time-, frequency-, or use-independent); and inward rectifier potassium current (IK1) (IC50 ≥ 30 &mgr;M). Dronedarone blocked L-type Ca2+ current (ICa(L)) (IC50 = 0.18 ± 0.018 &mgr;M at a stimulation frequency of 0.033 Hz) in a use- and frequency-dependent manner. Simultaneously to these electrophysiological effects, dronedarone reduced contraction amplitudes of papillary muscle and decreased Ca2+ transient and shortening of ventricular myocytes. The results show that dronedarone is a multichannel blocker because it decreases dV/dt max (INa), ICa(L), IKr, IKs, and IK1. These effects are accompanied by a reduction in free intracellular calcium and contraction amplitudes. Dronedarone does not significantly change APD whatever the stimulation frequency. Our data demonstrate that the acute electrophysiological characteristics of dronedarone, despite absence of iodine in its molecular structure, are very similar to those of amiodarone in cardiac ventricle.
Combining soft decisions of several unreliable experts

In this study we address the problem of integrating information from several experts with unknown levels of expertise. In the usual setup each expert expresses her opinion by choosing one of the options. Here we assume that each expert provides her opinion in a soft manner via a distribution on the possible options. The goal is to find the reliability level of each expert and to optimally integrate their information. We develop an estimation algorithm which is an instance of the EM algorithm and an efficiently computed approximation of the E-step. Finally we present simulations that demonstrate the improved performance of the proposed approach.
An objective methodology for infrared land surface emissivity evaluation

[1] Land surface emissivity (LSE) in the infrared (IR) window region (8–12 mm) governs the thermal emissions from the Earth’s surface. Many LSE databases, retrieved from various satellite instruments, are available for studying climate, Earth‐atmosphere interaction, weather, and the environment. The precision (standard deviation) and accuracy (bias) of these databases remain unclear. In this study, we introduce an objective and efficient method for quantitatively evaluating the LSE precision using satellite radiance observations. The LSE brightness temperature (Tb) deviations, defined as the standard deviations of Tb differences between satellite observations and radiative transfer calculations, can be estimated by minimizing the impacts from land surface temperature (LST) and atmospheric profiles. This is followed by the estimation of LSE precision. This method does not need the true LSE measurements. It only needs ancillary information such as atmospheric profiles and LST, both of which do not require high accuracy and thus can be obtained from a numerical weather prediction forecast or analysis. The method is applied to six different monthly LSE databases from August 2006 and 2007, and the results are presented. The error sources affecting the method are identified and the sensitivity to these errors is studied.
Geographic differences in the distribution of intestinal metaplasia in duodenal ulcer patients

OBJECTIVE:A strong correlation exists between atrophic gastritis and the intestinal type of gastric carcinoma. Duodenal ulcer disease characteristically has an antral predominant gastritis and a lower risk for gastric cancer. The aim of this study was to investigate the extent and distribution of intestinal metaplasia in duodenal ulcer in countries differing in gastric cancer incidence.METHODS:Topographically mapped gastric biopsy specimens (median 11) were obtained from patients with duodenal ulcer in four countries (Korea, Colombia, USA, and South Africa). Sections were stained with a triple stain and evaluated for Helicobacter pylori (H. pylori), active inflammation, and intestinal metaplasia.RESULTS:One hundred and sixty-five patients with duodenal ulcer were examined (29 from Korea, 52 from Colombia, 62 from the USA, and 22 from South Africa). The percentage of biopsies with intestinal metaplasia was significantly greater in Korean patients (86%) compared with that in other countries (50%) (p = 0.0004). Intestinal metaplasia was most prevalent in the antrum lesser curve and greater curve, and the body lesser curve. Intestinal metaplasia was present in the gastric corpus of 38% of duodenal ulcer patients from Korea compared with an average of 10% elsewhere (p = 0.018). No differences were observed in the density or distribution of H. pylori infection or in the degree of active gastritis between countries.CONCLUSIONS:Although antral predominant gastritis is the prevalent pattern of gastritis in duodenal ulcer, intestinal metaplasia in the gastric corpus may be found with geographic differences. These findings suggest that duodenal ulcer and gastric cancer are not mutually exclusive diseases but are rather ends of the spectrum of H. pylori infection.
Contact-dependent transfer of the galactose-specific lectin of Entamoeba histolytica to the lateral surface of enterocytes in culture

In a study to investigate early interactions of Entamoeba histolytica with epithelial cell monolayers, we found that a monoclonal antibody (MAb), CD6, against an ameba surface antigen recognized the lateral surface of epithelial cells after coculture with trophozoites. Display of the CD6 antigen on the epithelial cells necessitated contact with active trophozoites. It was found neither at 4 degrees C, nor with prefixed trophozoites, nor with trophozoite-conditioned media, nor when a filter prevented direct contact. Monolayers exposed to amebic sonicates or detergent lysates showed random immunostaining. Access to the antigenic site was limited, as immunostaining occurred predominantly with subconfluent monolayers. CD6 epithelial cell binding was first observed after 5 min of coculture; trophozoite-mediated target cell lysis was not detected until 15 min following coculture. Epithelial cell immunostaining occurred with some other ameba-specific antibodies but not with MAbs raised against the 170-kDa subunit of the galactose-N-acetylgalactosamine (Gal/GalNAc)-specific lectin. The CD6 MAb as well as some other ameba-specific antibodies immunoprecipitated from trophozoite lysates the same bands as the MAbs against the cysteine-rich domain of the 170-kDa subunit of the Gal/GalNAc-specific lectin. Why the latter MAbs failed to stain epithelial cells in the vicinity of attached trophozoites is presently unknown. We concluded that E. histolytica trophozoites transferred the intact amebic Gal/GalNAc-specific lectin or a portion of it to the lateral surface of epithelial cells. This juxtacrine transfer preceded killing of target cells.
Relationship Between Coronary Artery Calcification and Other Measures of Subclinical Cardiovascular Disease in Older Adults

Background—In the Cardiovascular Health Study, subclinical cardiovascular disease (CVD) predicted CVD events in older adults. The extent to which this measure or its components reflect calcified coronary disease is unknown. Methods and Results—Coronary artery calcium (CAC) was assessed with electron beam tomography in 414 participants without clinical CVD and examined using cut points (CAC≥400 and CAC≥800) and the log(CAC); 274 had subclinical CVD by ankle-arm index, ECG, or carotid ultrasound. Cut points for subclinical disease as previously defined in the Cardiovascular Health Study were examined as well as continuous measures to produce receiver operating characteristic curve curves. A low ankle-arm index was highly specific for a high CAC score. The internal carotid artery intima-media thickness was most strongly correlated with CAC (r =0.30) and was significantly related to both CAC cut points and to the log(CAC) score independently of all other measures. Conclusions—In these community-dwelling older adults without clinical CVD, internal carotid artery intima-media thickness was most closely related to CAC. However, 17.5% of those with a CAC≥400 would be missed in the ascertainment of subclinical atherosclerosis using the previously published composite of subclinical atherosclerosis. Prospective follow-up will determine whether the CAC score improves prediction of CVD events over other noninvasive measures.
Non-asymptotic confidence bounds for the optimal value of a stochastic program

We discuss a general approach to building non-asymptotic confidence bounds for Stochastic Optimization problems. Our principal contribution is the observation that a Sample Average Approximation of a problem supplies upper and lower bounds for the optimal value of the problem which are essentially better than the quality of the corresponding optimal solutions. At the same time, such bounds are more reliable than ‘standard’ confidence bounds obtained through the asymptotic approach. We also discuss bounding the optimal value of MinMax Stochastic Optimization and stochastically constrained problems. We conclude with a simulation study illustrating the numerical behaviour of the proposed bounds.
Be 13 , a Human T-Leukemia Cell Line Highly Sensitive to Dexamethasone-induced Cytolysis 1

A unique human T-leukemia cell line highly sensitive to dexamethasone-induced lysis is described. The cell line designated Be13 is killed readily within 24 hr by 10~9 M dexamethasone. No lysis is induced by nonglucocorticoid steroids. The lysis is me diated via specific cytoplasmic receptors and is efficiently blocked by the antagonist cortexolone. The inhibiting effect of actinomycin D and cycloheximide on the lytic process suggests the involvement of gene activation and destruction of the cells by an "autolytic protein. " Kinetic studies imply that the lytic process is induced during a distinct phase of the cell cycle. Dexamethasone, however, does not cause an arrest in a distinct phase of the cell cycle. The Be13 cell is a unique human cell line killed directly by glucocorticoids, and it may serve as a suitable in vitro model for studying the lytic effect of glucocorticoids on the proliferating compartment of human leukemias.
Wet or Dry – Hardness, Stiffness and Wear Resistance of Biological Materials on the Micron Scale

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Cancer, Comorbidities, and Health-Related Quality of Life of Older Adults

This study examined the physical and mental health of 126,685 males and females age 65 or over, with and without cancer that completed a Medicare Health Outcomes Survey (MHOS) between 1998-2002. Cancer information was ascertained through the National Cancer Institute's (NCI's) Surveillance, Epidemiology and End Results (SEER) program and linked to MHOS data. Results indicated that across most cancer types, cancer patients reported significantly more comorbid conditions and poorer physical and mental health compared with patients without cancer. Negative associations were most pronounced in those with two or more comorbidities and in those diagnosed with cancer within the past year.
Pediatric Endocrinologists' Management of Children With Type 2 Diabetes

OBJECTIVE To understand physician behaviors and attitudes in managing children with type 2 diabetes. RESEARCH DESIGN AND METHODS A survey was mailed to a nationwide sample of pediatric endocrinologists (PEs). RESULTS A total of 40% of PEs surveyed responded (211 of 527). Concordance with current monitoring guidelines varied widely, ranging from 36% (foot care) to 93% (blood pressure monitoring). Given clinical vignettes addressing hyperlipidemia, hypertension, and microalbuminuria, only 34% of PEs were fully concordant with current treatment guidelines. Reported barriers included concerns about patient adherence, insufficient scientific evidence about treatment, and lack of familiarity with current recommendations. Providers aged ≤45 years or in clinical practice <10 years reported significantly more aggressive management behaviors and had higher concordance with guidelines. CONCLUSIONS Screening and management of pediatric type 2 diabetes varied widely among PEs, suggesting opportunities for quality improvement. More aggressive management of type 2 diabetes among younger providers may be related to recent training when type 2 diabetes was more common.
What Do You Want in a Marriage? Examining Marriage Ideals in Taiwan and the United States

Four studies investigated ideal standards for one’s marital partner and relationship held by Taiwan Chinese and European Americans. We first generated a list of attributes that tapped lay representations of marriage ideals based on free responses from Chinese and European Americans, and we uncovered attributes describing extended family that were overlooked in Western research (Study 1). We found similar ideal knowledge structures across the two cultural groups; importantly, Chinese prioritized ideals denoting financial resources and extended family to a greater extent than did European Americans (Study 2). These cultural differences were explained by interdependent self-construal (Study 3). Finally, the agreement between ideals and perceptions of current partner/relationship was related to positive relationship outcomes in both cultural groups (Study 4). Our research highlights both cultural similarities and differences in the content, structure, endorsement, and evaluative functions of ideals in Chinese and Western cultural contexts.
Enhanced transfection of tumor cells in vivo using “Smart” pH-sensitive TAT-modified pegylated liposomes

Liposomes have been prepared loaded with DNA (plasmid encoding for the green fluorescent protein, GFP) and additionally modified with TATp and PEG, with PEG being attached to the liposome surface via both pH-sensitive hydrazone and non-pH-sensitive bonds. The pGFP-loaded liposomal preparations have been administered intratumorarly in tumor-bearing mice and the efficacy of tumor cell transfection was followed after 72 h. The administration of pGFP–TATp–liposomes with non-pH-sensitive PEG coating has resulted in only minimal transfection of tumor cells because of steric hindrances for the liposome-to-cell interaction created by the PEG coat, which shielded the surface-attached TATp. At the same time, the administration of pGFP–TATp–liposomes with the low pH-detachable PEG resulted in at least three times more efficient transfection since the removal of PEG under the action of the decreased intratumoral pH leads to the exposure of the liposome-attached TATp residues, enhanced penetration of the liposomes inside tumor cells and more effective intracellular delivery of the pGFP. This result can be considered as an important step in the development of tumor-specific stimuli-sensitive drug and gene delivery systems.
Changes in intra-abdominal visceral fat and serum leptin levels in patients with obstructive sleep apnea syndrome following nasal continuous positive airway pressure therapy.

BACKGROUND Obstructive sleep apnea syndrome (OSAS) is a common disorder in obese subjects. Visceral fat accumulation (VFA) is a better predictor of coronary heart disease than body mass index. Leptin is a hormone involved in the control of body weight and fat distribution. The effect of nasal continuous positive airway pressure (NCPAP) treatment on VFA and serum leptin levels in OSAS patients has not been known. METHODS AND RESULTS VFA and subcutaneous fat accumulation (SFA) were assessed by CT before and after NCPAP treatment in 22 OSAS patients (mean apnea and hypopnea index >50 episodes/h). Serum leptin levels of another 21 OSAS patients were measured before and after 3 to 4 days of NCPAP to gain insight into the mechanism by which NCPAP affects fat distribution. VFA and SFA decreased significantly after 6 months of NCPAP treatment (236+/-16 to 182+/-14cm(2), P=0.0003 and 215+/-21 to 189+/-18 cm(2), P=0.003, respectively). VFA decreased significantly in the body weight reduction group (n=9, P<0.01) and the no body weight reduction group (n=13, P<0.03). In contrast, SFA changed significantly in the body weight reduction group only (P<0.01). Leptin levels decreased significantly following 3 to 4 days of NCPAP (P<0.01), whereas body weight, fasting insulin, and cortisol levels did not change significantly. CONCLUSIONS Correction of sleep disordered breathing by NCPAP may be used to reduce VFA in OSAS patients. OSAS may have significant effects on the serum leptin levels.
Iterated Local search for the Linear Ordering Problem

This paper addresses the linear ordering problem, which has been solved using different metaheuristics approaches. These algorithms have the common problem of finding a proper balance of the intensification and diversification processes; in this work we propose an iterated local search in which it is incorporated a Becker heuristic strategy for constructing the initial solution, and a search strategy as perturbation process, achieving a better balance between intensification and diversification. The proposed algorithm obtained an improvement greater than 90%, decreasing the average percentage error respect the state of art ILS algorithm. The Wilcoxon nonparametric statistical test shows that the proposed algorithm significantly outperforms the iterated local search solution of the state of the art, ranking it among the top five solutions of the state of the art for the linear ordering problem.
Intermittent Fasting Applied in Combination with Rotenone Treatment Exacerbates Dopamine Neurons Degeneration in Mice

Intermittent fasting (IF) was suggested to be a powerful nutritional strategy to prevent the onset of age-related neurodegenerative diseases associated with compromised brain bioenergetics. Whether the application of IF in combination with a mitochondrial insult could buffer the neurodegenerative process has never been explored yet. Herein, we defined the effects of IF in C57BL/6J mice treated once per 24 h with rotenone (Rot) for 28 days. Rot is a neurotoxin that inhibits the mitochondrial complex I and causes dopamine neurons degeneration, thus reproducing the neurodegenerative process observed in Parkinson’s disease (PD). IF (24 h alternate-day fasting) was applied alone or in concomitance with Rot treatment (Rot/IF). IF and Rot/IF groups showed the same degree of weight loss when compared to control and Rot groups. An accelerating rotarod test revealed that only Rot/IF mice have a decreased ability to sustain the test at the higher speeds. Rot/IF group showed a more marked decrease of dopaminergic neurons and increase in alpha-synuclein (α-syn) accumulation with respect to Rot group in the substantia nigra (SN). Through lipidomics and metabolomics analyses, we found that in the SN of Rot/IF mice a significant elevation of excitatory amino acids, inflammatory lysophospholipids and sphingolipids occurred. Collectively, our data suggest that, when applied in combination with neurotoxin exposure, IF does not exert neuroprotective effects but rather exacerbate neuronal death by increasing the levels of excitatory amino acids and inflammatory lipids in association with altered brain membrane composition.
X-tolerant compression and application of scan-atpg patterns in a bist architecture

We present X-tolerant deterministic BIST (XDBIST), a novel method to efficiently compress and apply scan pattems generated by automatic test pattern generation (ATPG) in a logic built-in self-test architecture. Our method allows test patterns to have any number of unknown values with no degradation in compression and application efficiency. XDBIST does not require changing the core logic of the device under test (DUT); no test points or X-blockage logic need be inserted. The proposed solution guarantees the same high test coverage and diagnosis ability as deterministic scan-ATPG and uses the same tester flow, while reducing test data volume and tester cycles by more than 10 times.
View maintenance in a warehousing environment

A warehouse is a repository of integrated information drawn from remote data sources. Since a warehouse effectively implements materialized views, we must maintain the views as the data sources are updated. This view maintenance problem differs from the traditional one in that the view definition and the base data are now decoupled. We show that this decoupling can result in anomalies if traditional algorithms are applied. We introduce a new algorithm, ECA (for "Eager Compensating Algorithm"), that eliminates the anomalies. ECA is based on previous incremental view maintenance algorithms, but extra "compensating" queries are used to eliminate anomalies. We also introduce two streamlined versions of ECA for special cases of views and updates, and we present an initial performance study that compares ECA to a view recomputation algorithm in terms of messages transmitted, data transferred, and I/O costs.
Biosysthesis of Corn Starch Palmitate by Lipase Novozym 435

Esterification of starch was carried out to expand the usefulness of starch for a myriad of industrial applications. Lipase B from Candida antarctica, immobilized on macroporous acrylic resin (Novozym 435), was used for starch esterification in two reaction systems: micro-solvent system and solvent-free system. The esterification of corn starch with palmitic acid in the solvent-free system and micro-solvent system gave a degree of substitution (DS) of 1.04 and 0.0072 respectively. Esterification of corn starch with palmitic acid was confirmed by UV spectroscopy and IR spectroscopy. The results of emulsifying property analysis showed that the starch palmitate with higher DS contributes to the higher emulsifying property (67.6%) and emulsion stability (79.6%) than the native starch (5.3% and 3.9%). Modified starch obtained by esterification that possesses emulsifying properties and has long chain fatty acids, like palmitic acid, has been widely used in the food, pharmaceutical and biomedical applications industries.
Molecular Neurodegeneration Differential Effects of 24-hydroxycholesterol and 27-hydroxycholesterol on Β-amyloid Precursor Protein Levels and Processing in Human Neuroblastoma Sh-sy5y Cells

Background: Activation of the liver × receptors (LXRs) by exogenous ligands stimulates the degradation of β-amyloid 1–42 (Aβ42), a peptide that plays a central role in the pathogenesis of Alzheimer's disease (AD). The oxidized cholesterol products (oxysterols), 24-hydroxycholesterol (24-OHC) and 27-hydroxycholesterol (27-OHC), are endogenous activators of LXRs. However, the mechanisms by which these oxysterols may modulate Aβ42 levels are not well known.