Miracle-dz/UniFAR-FTUs huggingface dataset:
A New Monoclonal Antibody, mAb 4A12, Identifies a Role for the Glycosaminoglycan (GAG) Binding Domain of RANTES in the Antiviral Effect against HIV-1 and Intracellular Ca2+ Signaling

The β-chemokine RANTES (regulated on activation, normal T cell expressed and secreted) suppresses the infection of susceptible host cells by macrophage tropic strains of HIV-1. This effect is attributed to interactions of this chemokine with a 7-transmembrane domain receptor, CCR5, that is required for virus–cell fusion and entry. Here we identify domains of RANTES that contribute to its biological activities through structure–function studies using a new monoclonal antibody, mAb 4A12, isolated from mice immunized with recombinant human RANTES. This monoclonal antibody (mAb) blocked the antiviral activity of RANTES in infectivity assays with HIV-1Bal, and inhibited the mobilization of intracellular Ca2+ elicited by RANTES, yet recognized this chemokine bound to cell surfaces. Epitope mapping using limited proteolysis, reversed phase high-performance liquid chromatography, and mass spectrometry suggest that residues 55–66 of RANTES, which include the COOH-terminal α-helical region implicated as the glycosaminoglycan (GAG) binding domain, overlap the determinant recognized by mAb 4A12. This is supported by affinity chromatography studies, which showed that RANTES could be eluted specifically by heparin from a mAb 4A12 immunoaffinity matrix. Removal of cell surface GAGs by enzymatic digestion greatly reduced the ability of mAb 4A12 to detect RANTES passively bound on cell surfaces and abrogated the ability of RANTES to elicit an intracellular Ca2+ signal. Taken together, these studies demonstrate that the COOH-terminal α-helical region of RANTES plays a key role in GAG-binding, antiviral activity, and intracellular Ca2+ signaling and support a model in which GAGs play a key role in the biological activities of this chemokine.
Toxic effect of chemotherapy dosing using actual body weight in obese versus normal-weight patients: a systematic review and meta-analysis.

BACKGROUND Many oncologists reduce chemotherapy doses in obese patients due to fear of excess toxic effect from very large weight-based calculations. While recent guidelines advise against this practice, quantitative summarization of the supporting evidence is not available. MATERIALS AND METHODS We systematically identified studies that compared toxic effect or survival outcomes between obese and normal-weight adults receiving chemotherapy dosed by actual body weight (ABW). We pooled odds ratios (OR) and 95% confidence intervals (CI) using random-effects models. RESULTS Of 5490 records screened, 12 studies representing 9314 relevant patients met inclusion criteria. The large majority of reported toxic effect and survival outcomes did not statistically differ between obese and normal-weight subjects. Exceptions included five studies in which one or more toxic effect or survival outcomes statistically favored obese patients, and one study that statistically favored normal-weight patients. Pooling usable data, rates of toxic effects were similar or lower in obese patients (grade 3/4 hematologic toxic effect: OR 0.73, CI 0.55-0.98, 4 studies; grade 3/4 nonhematologic toxic effect: OR 0.98, CI 0.76-1.26, 3 subgroups; any grade 3/4 toxic effect: OR 0.75, CI 0.65-0.87, three studies). CONCLUSIONS Obese patients receiving chemotherapy based on ABW experience similar or lower rates of toxic effects compared with normal-weight patients, and survival outcomes do not differ.
Tandem ChoRE and CCAAT Motifs and Associated Factors Regulate Txnip Expression in Response to Glucose or Adenosine-Containing Molecules

Background Thioredoxin interacting protein (Txnip) is a multifunctional protein involved in regulation of cell cycle events and cellular metabolism. The expression of Txnip is known to be induced by glucose, adenosine-containing molecules, and other physiological cues; however, the underlying regulatory mechanisms remain elusive. Methodology/Principal Findings In this study, using promoter reporter, electrophoresis mobility shift (EMSA), and chromatin immuno-precipitation (ChIP) assays, we have identified an additional carbohydrate response element (ChoRE) on the promoter of Txnip gene, which functions cooperatively with the earlier identified ChoRE to mediate optimal Txnip expression. However, these two ChoREs are not sufficient to mediate the induction of Txnip expression by glucose or adenosine-containing molecules; and two CCAAT boxes, both of which can recruit nuclear factor Y (NF-Y) to the Txnip promoter, are also required for the induction. Accordingly, we have found that the function of ChoREs and associated factors is contingent on tandem CCAAT boxes, in that occupancy of the Txnip promoter by NF-Y is a prerequisite for efficacious recruitment of Mondo/MLX to ChoREs under glucose stimulation. Conclusions/Significance Our findings suggest a synergy between the tandem CCAAT and ChoRE motifs and associated NF-Y and Mondo/MLX transcription factors in enhancing transcription from the Txnip promoter. This piece of information will be helpful for future dissection of molecular mechanisms governing the transcriptional regulation of Txnip, a glucose responsive gene.
Low-Density Parity-Check codes for asymmetric distributed source coding

Low-Density Parity-Check (LDPC) codes achieve good performance, tending towards the Slepian-Wolf bound, when used as channel codes in Distributed Source Coding (DSC). Most LDPC codes found in literature are designed assuming random distribution of transmission errors. However, certain DSC applications can predict the error location within a certain level of accuracy. This feature can be exploited in order to design application specific LDPC codes to enhance the performance of traditional LDPC codes. This paper proposes a novel architecture for asymmetric DSC where the encoder is able to estimate the location of the errors within the side information. It then interleaves the bits having a high probability of error to the beginning of the codeword. The LDPC codes are designed to provide a higher level of protection to the front bits. Simulation results show that correct localization of errors pushes the performance of the system on average 13.3% closer to the Slepian-Wolf bound, compared to the randomly constructed LDPC codes. If the error localization prediction fails, such that the errors are randomly distributed, the performance is still in line with that of the traditional DSC architecture.
Quantitative Evaluation of Matching Methods and Validity Measures for Stereo Vision

The authors present a qualitative and quantitative comparison of various similarity measures that form the kernel of common area-based stereo-matching systems. The authors compare classical difference and correlation measures as well as nonparametric measures based on the rank and census transforms for a number of outdoor images. For robotic applications, important considerations include robustness to image defects such as intensity variation and noise, the number of false matches, and computational complexity. In the absence of ground truth data, the authors compare the matching techniques based on the percentage of matches that pass the left-right consistency test. The authors also evaluate the discriminatory power of several match validity measures that are reported in the literature for eliminating false matches and for estimating match confidence. For guidance applications, it is essential to have an estimate of confidence in the three-dimensional points generated by stereo vision. Finally, a new validity measure, the rank constraint, is introduced that is capable of resolving ambiguous matches for rank transform–based matching.
Surveying Wikipedia activity: Collaboration, commercialism, and culture

User generated content has grown drastically on the Internet over the last years. In this paper, we take a look at Wikipedia and compare Wikipedia editing activity both against commercial content producers as well as across different cultures. Our results show that commercial news sites have a clear diurnal and weekday-weekend patterns, whereas Wikipedia editing has a clear diurnal pattern, but no discernible weekday-weekend pattern. We studied 4 different Wikipedias from 4 different languages and cultures and found out that the editing behavior is very similar across all of them.
A Comparative Evaluation of the Dopamine D2/3 Agonist Radiotracer [11C](−)-N-Propyl-norapomorphine and Antagonist [11C]Raclopride to Measure Amphetamine-Induced Dopamine Release in the Human Striatum

(−)-N-Propyl-norapomorphine (NPA) is a full dopamine D2/3 receptor agonist, and [11C]NPA is a suitable radiotracer to image D2/3 receptors configured in a state of high affinity for agonists with positron emission tomography (PET). In this study, the vulnerability of the in vivo binding of [11C]NPA to acute fluctuation in synaptic dopamine was assessed with PET in healthy humans and compared with that of the reference D2/3 receptor antagonist radiotracer [11C]raclopride. Ten subjects (eight females and two males) were studied on two separate days, a minimum of 1 week apart, both with [11C]raclopride and [11C]NPA at baseline and after the administration of 0.5 mg · kg−1 oral d-amphetamine. Kinetic modeling with an arterial input function was used to derive the binding potential relative to nonspecific uptake (BPND) in the ventral striatum (VST), caudate (CAD), and putamen (PUT). [11C]Raclopride BPND was significantly reduced by 9.7 ± 4.4, 8.4 ± 4.2, and 14.7 ± 4.8% after amphetamine administration in the VST, CAD, and PUT. [11C]NPA BPND was also reduced significantly, by 16.0 ± 7.0, 16.1 ± 6.1, and 21.9 ± 4.9% after the same dose of amphetamine in the VST, CAD, and PUT. Although these results suggest that [11C]NPA is more vulnerable to endogenous competition by dopamine compared with [11C]raclopride by a factor of 1.49 to 1.90, the same data for a related outcome measure, binding potential relative to plasma concentration, was not significant. Nevertheless, these data add to the growing literature that suggests D2/3 agonist radiotracers are more vulnerable to endogenous competition by dopamine than existing D2/3 antagonist radiotracers.
Molecular Gas, Kinematics, and OB Star Formation in the Spiral Arms of the Southern Milky Way

The rotation curve for the fourth Galactic quadrant within the solar circle is derived from the Columbia University-Universidad de Chile CO (J = 1 → 0) survey of molecular gas. A new sampling, 4 times denser in longitude than in our previous analysis, is used to compute kinematical parameters that require derivatives with respect to galactocentric radius, the angular velocity Ω(R), the epicyclic frequency κ(R), and the parameters A(R) and B(R) describing, respectively, gas shear and vorticity. The face-on surface density of molecular gas is computed from the CO data in galactocentric radial bins for the subcentral vicinity, the same spectral region used to derive the rotation curve, where the twofold ambiguity in kinematical distances is minimum. The rate of massive star formation per unit area is derived for the same radial bins from the luminosity of IRAS pointlike sources with FIR colors of UC H II regions detected in the CS (J = 2 → 1) line. Massive star formation occurs preferentially in three regions of high molecular gas density, coincident with lines of sight tangent to spiral arms. The molecular gas motion in these arms resembles that of a solid body, characterized by constant angular velocity and by low shear and vorticity. The formation of massive stars in the arms follows the Schmidt law, ΣMSFR ∝ [Σgas]n, with an index of n = 1.2 ± 0.2. Our results suggest that the large-scale kinematics, through shear, regulate global star formation in the Galactic disk.
A Partial Test and Development of Delone and Mclean's Model of IS Success

DeLone and McLean's (1992) comprehensive review of different information system success measures concludes with a model of interrelationships between six IS Success constructs. This paper critically examines the meaning of four of these constructs and the evidence of relationships between them. It then provides results from empirical tests of these relationships. Tests are conducted using both conventional ordinary least squares regression path analysis and structural equation modeling - with substantially similar results. The empirical results provide substantial support for the "up stream" two thirds of DeLone and McLean's model. Three factors. System Quality, Information Quality, and Usefulness, are found to explain 75% of the variance in the overall User Satisfaction measure. The empirical results also provide substantial support for the use of usefulness as an IS success measure, and of the hitherto-unreported importance of "Importance of the task" in user perceptions of IS usefulness.
What Is Mathematics? An Exploration of Teachers' Philosophies of Mathematics during a Time of Curriculum Reform.

WHAT IS MATHEMATICS? AN EXPLORATION OF TEACHERS‘ PHILOSOPHIES OF MATHEMATICS DURING A TIME OF CURRICULUM REFORM by Kimberly White-Fredette Current reform in mathematics teaching and learning is rooted in a changing vision of school mathematics, one that includes constructivist learning, student-centered pedagogy, and the use of worthwhile tasks (National Council of Teachers of Mathematics, 1989, 1991, 2000). This changing vision not only challenges teachers‘ beliefs about mathematics instruction but their philosophies of mathematics as well (Dossey, 1992). This study investigates the processes that four teachers‘ go through as they implement a new task-based mathematics curriculum while exploring their personal philosophies of mathematics. The participants were part of a graduate-level course that examined, through the writings of Davis and Hersh (1981), Lakatos (1976), Polya (1945/1973), and others, a humanist/fallibilist philosophy of mathematics. These participants shared, through reflective writings and interviews, their struggles to, first, define mathematics and its purpose in society and in schools, and second, to reconcile their views of mathematics with their instructional practices. The study took place as the participants, two classroom teachers and two instructional coaches, engaged in the initial implementation of a reform mathematics curriculum, a reform based in social constructivist learning theories. Using narrative analysis, this study focuses on the unique mathematical stories of four experienced educators. Each of the participants initially expressed a traditional, a priori view of mathematics, seeing mathematics as right/wrong, black/white, a subject outside of human construction. The participants‘ expressed views of mathematics changed as they attempted to align their personal philosophies of mathematics with their (changing) classroom practices. They shared their personal struggles to redefine themselves as mathematics teachers through a process of experimentation, reflection, and adaptation. This process was echoed in their changing philosophies of mathematics. These participants came to see mathematics as fluid and a human construct; they also came to see their philosophies of mathematics as fluid and ever-changing, a process more than a product. WHAT IS MATHEMATICS? AN EXPLORATION OF TEACHERS‘ PHILOSOPHIES OF MATHEMATICS DURING A TIME OF CURRICULUM REFORM by Kimberly White-Fredette
Correlations for pairs of periodic trajectories for open billiards

In this paper we prove two asymptotic estimates for pairs of closed trajectories for open billiards similar to those established by Pollicott and Sharp (2006 Invent. Math. 163 10–24) for closed geodesics on negatively curved compact surfaces. The first of these estimates holds for general open billiards in any dimension. The more intricate second estimate is established for open billiards satisfying the so-called Dolgopyat type estimates. This class of billiards includes all open billiards in the plane and open billiards in (N ⩾ 3) satisfying some additional conditions.
Smoothing splines estimators for functional linear regression

The paper considers functional linear regression, where scalar responses $Y_1,. ,Y_n$ are modeled in dependence of random functions $X_1,. ,X_n$. We propose a smoothing splines estimator for the functional slope parameter based on a slight modification of the usual penalty. Theoretical analysis concentrates on the error in an out-of-sample prediction of the response for a new random function $X_{n+1}$. It is shown that rates of convergence of the prediction error depend on the smoothness of the slope function and on the structure of the predictors. We then prove that these rates are optimal in the sense that they are minimax over large classes of possible slope functions and distributions of the predictive curves. For the case of models with errors-in-variables the smoothing spline estimator is modified by using a denoising correction of the covariance matrix of discretized curves. The methodology is then applied to a real case study where the aim is to predict the maximum of the concentration of ozone by using the curve of this concentration measured the preceding day.
Student-Centered Teaching Meets New Media: Concept and Case Study

There exists empirical evidence proving that students who are given the freedom to explore areas based on their personal interests, and who are accompanied in their learning by a supportive, understanding facilitator, not only achieve superior academic results but also develop socially and grow personally. However, pure Student-Centered Teaching is more demanding in terms of communication, organization, as well as the provision of learning material. Thus, the basic idea underlying our paradigm is to combine Student-Centered Teaching with eLearning in order to exploit the advantages of the two approaches. We refer to this combined style as Student-Centered eLearning (SCeL). Strongly simplified, the computer takes over the task of providing information, while presence phases can be used for giving the content more meaning by means of transparent, open, respectful and empathic interactions within the group. Our case study indicates that Student-Centered eLearning has the potential of reducing the increased demands of Student-Centered Teaching in the long run, while fully retaining all its benefits, such as deeper learning processes, personal growth, social skills, and a higher degree of flexibility. Furthermore, the maturity for life-long learning is cultivated. In this paper we introduce our concept and derive first hypotheses on the conditions under which our paradigm appears most effective. While potential continuations of our studies are manifold, we intend to employ SCeL in the course of the new curriculum of the medical faculty of Graz University where a Virtual Medical Campus (http://vmc.uni-graz.at) is currently being developed.
Trunk muscle activation in a person with clinically complete thoracic spinal cord injury.

OBJECTIVE The aim of this study was to assess if, and how, upper body muscles are activated in a person with high thoracic spinal cord injury, clinically classified as complete, during maximal voluntary contractions and in response to balance perturbations. METHODS Data from one person with spinal cord injury (T3 level) and one able-bodied person were recorded with electromyography from 4 abdominal muscles using indwelling fine-wire electrodes and from erector spinae and 3 upper trunk muscles with surface electrodes. Balance perturbations were carried out as forward or backward support surface translations. RESULTS The person with spinal cord injury was able to activate all trunk muscles, even those below the injury level, both in voluntary efforts and in reaction to balance perturbations. Trunk movements were qualitatively similar in both participants, but the pattern and timing of muscle responses differed: upper trunk muscle involvement and occurrence of co-activation of ventral and dorsal muscles were more frequent in the person with spinal cord injury. CONCLUSION These findings prompt further investigation into trunk muscle function in paraplegics, and highlight the importance of including motor tests for trunk muscles in persons with thoracic spinal cord injury, in relation to injury classification, prognosis and rehabilitation.
Virtual Grand Rounds: A Curricular Model for Translating Evidence-Based Practice From the Classroom to the Clinic

Background and Purpose. e physical therapy profession supports educating physical therapist graduates to use evidencebased practice (EBP) skills in the clinical environment. Educational interventions to teach EBP include a variety of formats, but most focus on changing student and clinician knowledge and skills rather than changing actual clinician behavior. e literature suggests that for clinician behavior to change, educational interventions must be integrated into clinical practice. To describe the restructuring of the ATSU research and EBP curriculum including the development of a course, Virtual Grand Rounds (VGR), that focused on EBP skills and took place during clinical experiences. Method/Model Description and Evaluation. e curriculum and VGR course were designed for students to learn EBP skills, develop tolerance for uncertainty, implement EBP within time demands of the clinic, and use workplace supports. All physical therapy students completed both the Evidence-Based Practice Beliefs (EBPB) scale and the Evidence-Based Practice Implementation (EBPI) scale at program entry and at the end of years 1, 2, and 3 (graduation). Outcomes. A total of 727 EBPB and 719 EBPI surveys were completed and analyzed. Using an analysis of variance with Scheffe post hoc analyses, significant differences (P < .05) were found between the EBPB and EBPI scores for each year of data collection with the exception of a nonsignificant change between year 1 and year 2 (P = .998 and P = .702). e overall EBPI score at graduation indicated that during the final clinical year, students performed each EBP skill somewhere between 1 and 5 times during the clinical experience. Changes in individual question scores indicated increases in students’ skills, knowledge, and application throughout all years. Discussion and Conclusion. Evidencebased practice implementation increased throughout the 3 years and was at the highest following the final clinical year when students participated in the newly developed VGR. By situating practice of EBP within the time constraints of the clinical environment, the setting where EBP takes place, students are equipped to move away from the reliance on clinical expertise to evidence-based physical therapy. e development of the VGR course described in this article shifts EBP from an academic exercise in the classroom to an activity imbedded in real clinical practice. Other programs may find this integrated course helpful in removing barriers to EBP and developing graduates equipped to integrate EBP in the clinical environment.
Orchid Sexual Deceit Provokes Ejaculation

Sexually deceptive orchids lure pollinators by mimicking female insects. Male insects fooled into gripping or copulating with orchids unwittingly transfer the pollinia. The effect of deception on pollinators has been considered negligible, but we show that pollinators may suffer considerable costs. Insects pollinating Australian tongue orchids (Cryptostylis species) frequently ejaculate and waste copious sperm. The costs of sperm wastage could select for pollinator avoidance of orchids, thereby driving and maintaining sexual deception via antagonistic coevolution or an arms race between pollinator learning and escalating orchid mimicry. However, we also show that orchid species provoking such extreme pollinator behavior have the highest pollination success. How can deception persist, given the costs to pollinators? Sexually‐deceptive‐orchid pollinators are almost exclusively solitary and haplodiploid species. Therefore, female insects deprived of matings by orchid deception could still produce male offspring, which may even enhance orchid pollination.