Miracle-dz/UniFAR-FTUs huggingface dataset:
What Medical Students Say About Psychiatry: Results of a Reflection Exercise

ObjectiveThe author describes the results of a reflection exercise for psychiatry clerkship students.MethodsThe author performed a qualitative analysis on 100 “reflection” papers written by medical students in their psychiatry clerkship and identified the most prominent thematic content.ResultsThe most common thematic content involved social issues in psychiatry, the reality of mental illness, the role of trauma, difficulties forming alliances with patients, the efficacy of interventions, the power of empathic connection, and students’ personal identifications.ConclusionReflection exercises can foster students’ awareness of their preexisting attitudes toward mental illness and the evolution of their views during training. Educators can use reflection exercises to better appreciate their students’ concerns and target curriculum content toward specific issues.
Damages of skidder and animal logging to forest soils and natural regeneration.

Nacrtak Extracting logs from stump to landings causes extensive damages to forest stand and soil. In this research two parcels adjacent to each other were selected in order to assess the effect of traditional and mechanized methods of logging on regeneration and soil compaction. A skid trail and a mule trail with similar longitudinal slope, skidding direction and total volume of extracted wood were chosen in the parcels. The cylindrical sampling method was used to determine wet and dry soil bulk density and the samples were taken at 0–10 cm and 10–20 cm depth from skid and mule trails. The results showed that soil dry bulk density increase in skid and mule trails compared to control at 0–10 and 10–20 cm depth, was significant (p<0.01). This increase in mule trail at 0–10 cm depth was significantly higher than at 10–20 cm depth (p<0.01), but percentage of soil dry bulk density increase compared to control in skid trail at 0–10 cm and 10–20 cm depths was not significantly different. Soil dry bulk density increase compared to control at 0–10 cm depth of mule trail is higher than skid trail, but at 10–20 cm depth the skid trail is higher than mule trail. Systematic random sampling method was used to determine damages to different regeneration groups due to logging operations. The results showed that damages to each regeneration group seedling and small sapling in mule logging method were significantly lower than mechanized logging method.
Quantitative analysis of intact apolipoproteins in human HDL by top-down differential mass spectrometry

Top-down mass spectrometry holds tremendous potential for the characterization and quantification of intact proteins, including individual protein isoforms and specific posttranslationally modified forms. This technique does not require antibody reagents and thus offers a rapid path for assay development with increased specificity based on the amino acid sequence. Top-down MS is efficient whereby intact protein mass measurement, purification by mass separation, dissociation, and measurement of product ions with ppm mass accuracy occurs on the seconds to minutes time scale. Moreover, as the analysis is based on the accurate measurement of an intact protein, top-down mass spectrometry opens a research paradigm to perform quantitative analysis of “unknown” proteins that differ in accurate mass. As a proof of concept, we have applied differential mass spectrometry (dMS) to the top-down analysis of apolipoproteins isolated from human HDL3. The protein species at 9415.45 Da demonstrates an average fold change of 4.7 (p-value 0.017) and was identified as an O-glycosylated form of apolipoprotein C-III [NANA-(2 → 3)-Gal-β(1 → 3)-GalNAc, +656.2037 Da], a protein associated with coronary artery disease. This work demonstrates the utility of top-down dMS for quantitative analysis of intact protein mixtures and holds potential for facilitating a better understanding of HDL biology and complex biological systems at the protein level.
Applying mobile device soft keyboards to collaborative multitouch tabletop displays: design and evaluation

We present an evaluation of text entry methods for tabletop displays given small display space allocations, an increasingly important design constraint as tabletops become collaborative platforms. Small space is already a requirement of mobile text entry methods, and these can often be easily ported to tabletop settings. The purpose of this work is to determine whether these mobile text entry methods are equally useful for tabletop displays, or whether there are unique aspects of text entry on large, horizontal surfaces that influence design. Our evaluation consists of two studies designed to elicit differences between the mobile and tabletop domains. Results show that standard soft keyboards perform best, even at small space allocations. Furthermore, occlusion-reduction methods like Shift do not yield significant improvements to text entry; we speculate that this is due to the low ratio of resolution per surface units (i.e., DPI) for current tabletops.
The Glitches of the Anomalous X-Ray Pulsar 1RXS J170849.0–400910

We report on a timing analysis of archival observations of the anomalous X-ray pulsar 1RXS J170849.0-400910 made with the RXTE Proportional Counter Array. We detect a new large glitch (Δν/ν ≃ 3 × 10-6) that occurred between 2001 March 27 and May 6, with an associated large increase in the spin-down rate (Δ/ ≃ 0.3). The short time (1.5 yr) elapsed from the previously detected glitch and the large amplitude of the new spin-up place this source among the most frequent glitchers, with large average glitch amplitudes, similar to those of the Vela pulsar. The source shows different recoveries after the glitches: in the first one it is well described by a long-term linear trend similar to those seen in Vela-like glitches; in the second case the recovery is considerably faster and is better described by an exponential plus a fractional change in the long-term spin-down rate of the order of 1%. No recovery of the latter is detected, but additional observations are necessary to confirm this result. We find minor but significant changes in the average pulse profile after both glitches. No bursts were detected in any light curve, but our search was limited in sensitivity with respect to short (t < 60 ms) bursts. Observed glitch properties are compared to those of radio pulsar glitches; current models are discussed in light of our results. It appears that glitches may represent yet another peculiarity of anomalous X-ray pulsars. Starquake-based models appear to be preferred on qualitative grounds. Alternative models can be applied to individual glitches but fail in explaining both. Thus the two events may as well arise from two different mechanisms.
A delay-fractioning approach to stability analysis of networked control systems with time-varying delay

This paper concerns the establishment of a new stability criterion for networked control systems (NCSs) liable to model uncertainties and time-varying delays. The proposed criterion is an improvement over previous ones, for the employment of a novel delay-fractioning approach and the development of a new Lyapunov-Krasovskii functional (LKF). The analysis incorporates state-of-the-art stability techniques for systems with time-varying delays such as convex optimization technique and piecewise analysis method. Moreover, we consider the derivative character of the NCS' time-varying delay. The analysis is enriched with numerical examples that illustrates the effectiveness of the proposed criterion which outperform state-of-the-art stability criteria in the literature for nominal and uncertain NCSs.
The potential role of hybridization in diversification and speciation in an insular plant lineage: insights from synthetic interspecific hybrids

Islands contain some of the most remarkable yet imperiled plants anywhere on Earth. Despite remarkable ecological/morphological diversity, insular endemics often retain the ability to hybridize and produce vigorous, fertile progeny. This study over several generations of synthetic interspecific hybrids between two species endemic to the Canary Islands shows that gene exchange between species can produce novel traits, transgressive phenotypes and novel combinations of features not seen in their parents. Results of this study provide experimental evidence of the potential of hybridization to promote diversification and possibly generate stabilized hybrid derivatives in plants in an oceanic archipelago.
Optimization of Donor Management Goals Yields Increased Organ Use

Multiple strategies have been used in an effort to increase the pool of organs for transplantation. Standardizing donor management has produced promising results. Donor management goals (DMGs) are now being used as end points of intensive care unit care during the prerecovery phase but no prospective results have been reported. Data from the United Network for Organ Sharing Region 11 were collected for successful achievement of eight common donor management goals (mean airway pressure [MAP], central venous pressure [CVP], pH, PaO2, sodium, glucose, single pressor use, and urine output) before organ recovery. Two time periods were studied with different panels of DMGs. The analysis identified the success rate of transplantation. Goals were stratified by their statistical correlation with the number of organs transplanted per donor (OTPD) in an effort to identify the most important parameter(s). Eight hundred five organ donors were studied with 2685 organs transplanted. DMGs were assessed through two phases of the study. Achieving DMGs rose from 18 to 66 per cent associated with significant improvement in OTPD (range, 2.96 to 3.45). The success of transplantation was primarily associated with limitations in vasopressor use and PaO2. Tight glucose control did affect the rate of pancreatic transplants. Thoracic organs were the most sensitive to DMGs with a 10- to 15-fold increase in lung transplantation when PaO2 rose above 100 mmHg. MAP, CVP, pH, sodium, and urine output had little effect on transplantation. Standardization of end points of donor management was associated with increased rates of transplantation. Surprisingly, not all standard goals are necessary for optimal organ use. The most significant parameters were the low use of vasopressor agents and oxygenation. Donor management strategies should strive to optimize these goals.
Functional GABAergic Synaptic Connection in Neonatal Mouse Barrel Cortex

Intracortical inhibition is crucial to proper functioning of the mature neocortex, yet, paradoxically, is reported to be rare or absent in the neonatal animal. We reexamined this issue by recording whole-cell postsynaptic currents (PSCs) of barrel cortex neurons in thalamocortical brain slices from neonatal mice. Monosynaptic, excitatory thalamocortical responses were elicited in layers V/VI neurons as early as postnatal day 0 (P0, the first 24 hr after birth) and in presumptive layer IV as early as P2. At very low stimulation frequencies, the monosynaptic response was invariably followed by a prolonged (up to 1 sec) synaptic barrage, which fatigued at stimulus repetition rates of 2/min or higher. This barrage consisted of postsynaptic responses to spiking activity in neighboring cortical cells, because (1) it could also be evoked by intracortical stimulation in coronal slices and (2) it was abolished by antagonists to NMDA receptors (NMDARs), even when NMDARs on the recorded cell were under a voltage-dependent block. Some of the larger polysynaptic events changed polarity at a negative reversal potential and were blocked by GABAA receptor (GABAAR) antagonists, with a concurrent enhancement of the extracellular field potential, indicating that they were GABAAR- mediated, Cl-dependent inhibitory PSCs (IPSCs). We conclude that a network of functional intracortical GABAAR-mediated synaptic connections exists from the earliest postnatal ages, although it gives rise to responses that differ from mature IPSCs in reversal potential and latency.
Wage and Price Dynamics in Portugal

This paper investigates the persistence of aggregate wages and prices in Portugal assuming a model of a unionized economy with imperfect competition. An impulse response analysis is conducted where the structural shocks are identified by taking into account the long-run properties of the model, as well as the cointegrating and weak-exogeneity properties of the system. Real wages and wage inflation emerge as especially persistent following an import price shock, while price inflation is more persistent following an unemployment shock. At the business cycle horizon variation in the forecast errors of wages is attributable mainly to unemployment shocks (about 80 percent), whereas variation in the forecast errors of prices is attributable mainly to import price shocks (about 60 percent) and to unemployment shocks (around 20 percent). Productivity shocks explain somewhat less than 10 percent of the variation in forecast errors of wages and prices.
Tracking control using attitude measurements for flexible spacecraft

The problem of tracking a desired attitude is here addressed in the case of a flexible spacecraft subject to environmental disturbances. This problem is solved in a practical way for attitude maneuvers with bounded velocity and acceleration and for disturbances with bounded dynamics, ensuring the ultimate boundedness of the system trajectories. The variables available for feedback are those describing the spacecraft attitude. The unmeasured variables, such as the angular velocity and the modal position and velocity, along with the disturbance acting on the spacecraft, are reconstructed by means of appropriate dynamics.
Soil ecotoxicology: state of the art and future directions

Abstract Developments in soil ecotoxicology started with observations on pesticide effects on soil invertebrates in the 1960s. To support the risk assessment of chemicals, in the 1980s and 1990s development of toxicity tests was the main issue, including single species tests and also more realistic test systems like model ecosystems and field tests focusing on structural and functional endpoints. In the mean time, awareness grew about issues like bioavailability and routes of exposure, while biochemical endpoints (biomarkers) were proposed as sensitive and potential early-warning tools. In recent years, interactions between different chemicals (mixture toxicity) and between chemical and other stressors attracted scientific interest. With the development of molecular biology, omics tools are gaining increasing interest, while the ecological relevance of exposure and effects is translating into concepts like (chemical) stress ecology, ecological vulnerability and trait-based approaches. This contribution addresses historical developments and focuses on current issues in soil ecotoxicology. It is concluded that soil ecotoxicological risk assessment would benefit from extending the available battery of toxicity tests by including e.g. isopods, by paying more attention to exposure, bioavailability and toxicokinetics, and by developing more insight into the ecology of soil organisms to support better understanding of exposure and long-term consequences of chemical exposure at the individual, population and community level. Ecotoxicogenomics tools may also be helpful in this, but will require considerable further research before they can be applied in the practice of soil ecotoxicological risk assessment.
Investigating the Responses of Cronobacter sakazakii to Garlic-Drived Organosulfur Compounds: a Systematic Study of Pathogenic-Bacterium Injury by Use of High-Throughput Whole-Transcriptome Sequencing and Confocal Micro-Raman Spectroscopy

ABSTRACT We present the results of a study using high-throughput whole-transcriptome sequencing (RNA-seq) and vibrational spectroscopy to characterize and fingerprint pathogenic-bacterium injury under conditions of unfavorable stress. Two garlic-derived organosulfur compounds were found to be highly effective antimicrobial compounds against Cronobacter sakazakii, a leading pathogen associated with invasive infection of infants and causing meningitis, necrotizing entercolitis, and bacteremia. RNA-seq shows changes in gene expression patterns and transcriptomic response, while confocal micro-Raman spectroscopy characterizes macromolecular changes in the bacterial cell resulting from this chemical stress. RNA-seq analyses showed that the bacterial response to ajoene differed from the response to diallyl sulfide. Specifically, ajoene caused downregulation of motility-related genes, while diallyl sulfide treatment caused an increased expression of cell wall synthesis genes. Confocal micro-Raman spectroscopy revealed that the two compounds appear to have the same phase I antimicrobial mechanism of binding to thiol-containing proteins/enzymes in bacterial cells generating a disulfide stretching band but different phase II antimicrobial mechanisms, showing alterations in the secondary structures of proteins in two different ways. Diallyl sulfide primarily altered the α-helix and β-sheet, as reflected in changes in amide I, while ajoene altered the structures containing phenylalanine and tyrosine. Bayesian probability analysis validated the ability of principal component analysis to differentiate treated and control C. sakazakii cells. Scanning electron microscopy confirmed cell injury, showing significant morphological variations in cells following treatments by these two compounds. Findings from this study aid in the development of effective intervention strategies to reduce the risk of C. sakazakii contamination in the food production environment and on food contact surfaces, reducing the risks to susceptible consumers.
Screening of Biodegradable Function of Indigenous Ligno-degrading Mushroom Using Dyes

Abstract The process of biodegradation in lingo-eellulosie materials is critically relevant to biospheric carbon. The study of this natural process has largely involved laboratory investigations, focused primarily on the biodegradation and recycling of agricultural by-products, generally using basidiomyeetes species. In order to collect super white rot fungi and evaluate its ability to degrade lingo-eellulosie material, 35 fungal strains, collected from forests, humus soil, livestock manure, and dead trees, were screened for enzyme activities and their potential to decolorize the commercially used Poly-R 478 dye. In the laeease enzymatic analysis chemical test, 33 white rot fungi and 2 brown rot fungi were identified. The degradation ability of polycyclic aromatic hydrocarbons (PAHs) according to the utilized environmental conditions was higher in the mushrooms grown in dead trees and fallen leaves than in the mushrooms grown in humus soil and livestock manure. Using Poly-R 478 dye to assess the PAH-degradation activity of the identified strains, four strains, including Agrocybe pediades, were selected. The activities of laeease, MnP, and Lip of the four strains with PAH-degrading ability were highest in Pleurotus incarnates. 87 fungal strains, collected from forests, humus soil, livestock manure, and dead trees, were screened for enzyme activities and their potential to decolorize the commercially used Poly-R 478 dye on solid media. Using Poly-R 478 dye to assess the PAH-degrading activity of the identified strains, it was determined that MKACC 51632 and 52492 strains evidenced superior activity in static and shaken liquid cultures. Subsequent screening on plates containing the polymeric dye poly R-478, the deeolorization of which is correlated with lignin degradation, resulted in the selection of a strain of Coriolus versicolor, MKACC'52492, for further study, primarily due to its rapid growth rate and profound ability to decolorize poly R-478 on solid media. Considering our findings using Poly-R 478 dye to evaluate the PAH-degrading activity of the identified strains, Coriolus versicolor, MKACC 52492 was selected as a favorable strain. Coriolus versicolor, which was collected from Mt. Yeogi in Suwon, was studied for the production of the lignin-modifying enzymes laeease, manganese-dependent peroxidase (MnP), and lignin peroxidase (LiP).
Morphometric Study of Early Postnatal Development of the Thoracic Aorta in the Rat

Tissue, cellular, and subcellular morphometry of the thoracic aorta in 1-, 5-and 11-day-old rats was used to quantify the cellular hypertrophy and proliferation of smooth muscle cells and the absolute increases in volume of elastic laminae and collagen during the early postnatal period. In the 1-to 5-day interval, total wall volume increased 2.9-fold, wall thickness and the number of smooth muscle cells doubled, mean cell volume increased by 40%, and the volumes of elastic laminae and collagen increased 3.7-and 3.3-fold, respectively. From 5 to 11 days, circumferential growth of the aortic wall, without further thickening, produced smaller and unequal growth increments of each of its component structures, with increases in collagen (3.2-fold) > elastic laminae (2.3-fold) > muscle cells (1.6-fold). In the overall growth of smooth muscle cells (4.6-fold) from 1 to 11 days, only the cytoplasmic volume fractions of glycogen aggregates and rough endoplasmic reticulum were altered significantly (−78%, +32%). Certain aspects of normal postnatal aortic growth paralleled the response of adult aorta to experimentally induced hypertension. Circ Res 47: 417−424, 1980
A Role for LKP2 in the Circadian Clock of Arabidopsis Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010332.

A third member of the ZTL gene family was identified in the Arabidopsis genome and was named LKP2 (for LOV kelch protein2). A cDNA was isolated corresponding to this gene, and plants overexpressing LKP2 were generated. The overexpression of LKP2 resulted in arrhythmic phenotypes for a number of circadian clock outputs in both constant light and constant darkness, long hypocotyls under multiple fluences of both red and blue light, and a loss of photoperiodic control of flowering time. The LKP2 mRNA is not regulated by the circadian clock and was detected in all tissues examined. These results suggest that LKP2 functions either within or very close to the circadian oscillator in Arabidopsis. A model is presented for its mode of action.
Study of Meltblown Structures Formed by Robotic and Meltblowing Integrated System: Impact of Process Parameters on Fiber Orientation

A novel system that forms two-dimensional (2D) structures on a rotating drum and three-dimensional (3D) structures on 3D molds through proper integration of a laboratory scale meltblown unit with a small die and six-axis robot is briefly described. The system advantages over traditional systems are demonstrated. Parametric studies evaluating the effect of take-up speed, die-to-collector-distance (DCD), polymer throughput rate, and attenuating air pressure on the fiber orientation distribution functions (ODFs) of 2D structures formed by the system are reported. An additional new parameter termed “fiber-stream approach-angle” is introduced and its impact on the ODF of 2D structures is also reported. Under the experimental range studied, the ODFs were significantly impacted by the parameters studied. The fiber-stream approach angle showed the highest impact, among the parameter studied, on the ODF.