Miracle-dz/UniFAR-FTUs huggingface dataset:
Metabolomics‐on‐a‐chip approach to study hepatotoxicity of DDT, permethrin and their mixtures

Despite the diversity of studies on pesticide toxicities, there is a serious lack of information concerning the toxic effect of pesticides mixtures. Dichlorodiphenyl‐trichloroethane (DDT) and permethrin (PMT) are among the most prevalent pesticides in the environment and have been the subject of several toxicological studies. However, there are no data on the toxicity of their mixtures. In this study, we used an approach combining cell culture in microfluidic biochips with gas chromatography–mass spectrometry metabolomics profiling to investigate the biomarkers of toxicity of DDT, PMT and their mixtures. All parameters observed indicated that no significant effect was observed in hepatocytes cultures exposed to low doses (15 μm) of DDT and PMT. Conversely, combined low doses induce moderate oxidative stress and cell death. The toxic signature of high doses of pesticides (150 μm) was illustrated by severe oxidative stress and cell mortality. Metabolomics profiling revealed that hepatocytes exposure to DDT150, PMT150 and DDT150 and PMT150 cause important modulation in intermediates of glutathione pathway and tricarboxylic acid cycle, amino acids and metabolites associated to hepatic necrosis and inflammation (α‐ketoglutarate, arginine and 2‐hydroxybutyrate). These changes were more striking in the combined group. Finally, DDT150 led to a significant increase of benzoate, decanoate, octanoate, palmitate, stearate and tetradecanoate, which illustrates the estrogen modulation. This study demonstrates the potential of metabolomics‐on‐a‐chip approach to improve knowledge on the mode of action of pesticides.
Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence

Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, represents one of the main causes of abortion in cattle. Macrophages (MØs) are mediators of the innate immune response against infection and likely one of the first cells encountered by the parasite during the host infection process. In this study, we investigated in vitro how high or low virulent isolates of N. caninum (Nc-Spain7 and Nc-Spain1H, respectively) interact with bovine monocyte-derived MØs and the influence of the isolate virulence on the subsequent cellular response. Both isolates actively invaded, survived and replicated in the MØs. However, Nc-Spain7 showed a higher invasion rate and a replication significantly faster, following an exponential growth model, whereas Nc-Spain1H presented a delayed replication and a lower growth rate without an exponential pattern. N. caninum infection induced a hypermigratory phenotype in bovine MØs that was characterized by enhanced motility and transmigration in vitro and was accompanied by morphological changes and abrogated extracellular matrix degradation. A significantly higher hypermotility was observed with the highly virulent isolate Nc-Spain7. Nc-Spain1H-infected MØs showed elevated reactive oxygen species (ROS) production and IL12p40 expression, which also resulted in increased IFN-γ release by lymphocytes, compared to cells infected with Nc-Spain7. Furthermore, IL-10 was upregulated in MØs infected with both isolates. Infected MØs exhibited lower expression of MHC Class II, CD86, and CD1b molecules than uninfected MØs, with non-significant differences between isolates. This work characterizes for the first time N. caninum replication in bovine monocyte-derived MØs and details isolate-dependent differences in host cell responses to the parasite.
Transcranial direct current stimulation (tDCS) targeting the left inferior frontal gyrus: Effects on creativity across cultures

ABSTRACT Although evidence points to the existence of cultural differences in creativity, the mechanisms that underlie these differences are largely unknown. The twofold model of creativity posits two recursive stages: a generation phase and an evaluation phase. Considering that recent studies suggest that the left inferior frontal gyrus) L-IFG(may inhibit creativity during the evaluation phase, we hypothesized that manipulating IFG activity would affect participants’ level of creativity. We further predicted that the IFG may be “hyperactive” in a culture that typically discourages uniqueness (e.g., Japan) as compared to a Western culture (e.g., Israel); therefore the effect of inhibiting the L-IFG was expected to be higher in Japanese. To support this hypothesis we altered L-IFG activation using tDCS (transcranial direct current stimulation) in 30 Japanese and 30 Israeli participants and examined the effect of anodal and cathodal stimulation on divergent thinking (DT). Regardless of culture, cathodal stimulation increased creativity compared to sham, while anodal stimulation diminished creativity. Although cultural differences were found in DT scores, stimulation had no differential effect between the cultures. Taken together, the results confirm that the L-IFG plays a key role in creativity across cultures. The null findings regarding the expected interaction between stimulation and culture are discussed.
Replacing sugary drinks with milk is inversely associated with weight gain among young obesity-predisposed children

Abstract The aim of the present study was to examine the associations of sugary drink consumption and its substitution with alternative beverages with body weight gain among young children predisposed to future weight gain. Secondary analysis of the Healthy Start Study, a 1·5-year randomised controlled trial designed to prevent overweight among Danish children aged 2−6 years (n 366), was carried out. Multivariate linear regression models were used to investigate the associations of beverage consumption with change in body weight (Δweight) or BMI(ΔBMI) z-score. Substitution models were used to extrapolate the influence of replacing sugary drinks with alternative beverages (water, milk and diet drinks) on Δweight or ΔBMI z-score. Sugary drink intake at baseline and substitution of sugary drinks with milk were associated with both Δweight and ΔBMI z-score. Every 100 g/d increase in sugary drink intake was associated with 0·10 kg and 0·06 unit increases in body weight (P=0·048) and BMI z-score (P=0·04), respectively. Substitution of 100 g/d sugary drinks with 100 g/d milk was inversely associated with Δweight (β=−0·16 kg; P=0·045) and ΔBMI z-score (β=−0·07 units; P=0·04). The results of this study suggest that sugary drink consumption was associated with body weight gain among young children with high predisposition for future overweight. In line with the current recommendations, sugary drinks, whether high in added or natural sugar, should be discouraged to help prevent childhood obesity. Milk may be a good alternative to sugary drinks with regard to weight management among young obesity-predisposed children.
Relation of Retinopathy of Prematurity to Brain Volumes at Term Equivalent Age and Developmental Outcome at 2 Years of Corrected Age in Very Preterm Infants

Background: Retinopathy of prematurity (ROP) is a major complication of preterm birth and has been associated with later visual and nonvisual impairments. Objectives: To evaluate relationships between any stage of ROP, brain volumes, and developmental outcomes. Methods: This study included 52 very preterm infants (gestational age [mean ± SD]: 26.4 ± 1.9 weeks). Total brain, gray matter, unmyelinated white matter (UWMV), and cerebellar volumes were estimated in 51 out of 52 infants by magnetic resonance imaging at term-equivalent age. Bayley Scales of Infant Development were used to assess developmental outcomes in 49 out of 52 infants at a mean corrected age of 24.6 months. Results: Nineteen out of 52 infants developed any stage of ROP. Infants with ROP had a lower median (IQR) UWMV (173 [156–181] vs. 204 [186–216] mL, p < 0.001) and cerebellar volume (18.3 [16.5–20] vs. 22.3 [20.3–24.7] mL, p < 0.001) than infants without ROP. They also had a lower median (IQR) mental developmental index (72 [56–83] vs. 100 [88–104], p < 0.001) and a lower psychomotor developmental index (80 [60–85] vs. 92 [81–103], p = 0.002). Brain volumes and developmental outcomes did not differ among infants with different stages of ROP. Conclusion: Any stage of ROP in preterm infants was associated with a reduced brain volume and an impaired developmental outcome. These results suggest that common pathways may lead to impaired neural and neurovascular development in the brain and retina and that all stages of ROP may be considered in future studies on ROP and development.
Hollow Silver Nanostructures: The Role of Capping Agents in Tailoring the Shape, Structure, and Plasmonic Properties

Abstract The shape- and structure-directing ability of capping agents, namely, acetic acid (AA) and folic acid (FA), has been analyzed in the synthesis of hollow plasmonic nanostructures via the nanoscale Kirkendall effect. FA was found to possess both shape-directing and structure-directing abilities when spherical solid Ag2O nanoparticles were transformed into hollow silver nanocubes (HAgNCs). In contrast, AA acted only as a structure-directing agent in the transformation from solid Ag2O nanospheres to hollow Ag nanospheres (HAgNSs). FA capping leads to enhanced plasmon tunability range from 535 to 640 nm in the hollow silver nanostructures. The size and shape of nanostructures were analyzed by high-resolution transmission electron microscopy (HRTEM). HRTEM revealed that the outer diameter of AA-capped HAgNSs is 50 ± 10 nm while edge-length for FA-capped HAgNCs is 100 ± 15 nm. The diameter of inner void space was found to be 30 ± 5 and 43 ± 5 nm for HAgNSs and HAgNCs, respectively. The phase purity of the hollow nanostructures was confirmed by X-ray diffraction and energy dispersive X-ray measurements. Due to unique structural and plasmonic features, FA-capped HAgNCs are well-suited for biomedical applications.
Lean Mass Declines Consistently over 10 Years in People living with HIV on Antiretroviral Therapy, with Patterns Differing by Sex

Background The long-term trajectory of and factors affecting lean mass in people living with HIV (PLWH) are incompletely described. Methods PLWH in the Modena HIV Metabolic Cohort underwent dual-energy X-ray absorptiometry (DXA) scans every 6–12 months for up to 10 years (median 4.6 scans). Mixed effect regression modelling in combined and sex-stratified models determined annual rates of and clinical factors significantly associated with appendicular lean mass (ALM). Results A total of 839 women and 1,759 men contributing ≥2 DXA scans had baseline median age 44 years and 14 years since HIV diagnosis; 76% were virologically suppressed on antiretroviral therapy (ART). Baseline median ALM was 16.9 kg for women and 24.8 kg for men. ALM decreased during the study period, with mean yearly ALM loss of −231 g in women and −322 g in men. Less ALM was associated with female sex, age >50 years, detectable HIV-1 RNA, and tenofovir and integrase inhibitor use. Greater ALM was associated with longer ART duration. In sex-stratified models, relationships between ALM and total ART duration and integrase inhibitor use were not significant for women, but the relationship with tenofovir use persisted. For men, AIDS wasting and CD4+ T-lymphocyte nadir <200 cells/ml were independently associated with lower ALM. Conclusions ALM steadily declined over time in this cohort of PLWH on ART that included a large number of women. HIV- and ART-specific risk factors emerged that varied by sex. The observed associations between tenofovir or integrase inhibitor use and lower ALM particularly warrant further study.
Determination of genetic stability of micropropagated plants of ginger using Random Amplified Polymorphic DNA (RAPD) markers

Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of micropropagated plants of Zingiber officinales cv. V 3 S 18 . Fifteen arbitrary decamers were used to amplify DNA from in vivo and in vitro plant material to assess the genetic fidelity. All RAPD profiles from micropropagated plants were monomorphic and similar to those of field grown control plants. No variation was detected within the micropropagated plants. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the systems of micropropagation is discussed.
Surface Segregation of Cyclic Chains in Binary Melts of Thin Polymer Films: The Influence of Constituent Concentration

We carry out extensive molecular dynamics simulations of thin films of bead-spring models of binary mixtures composed of cyclic and linear polymer chains. We study the equilibrium behavior of the polymer chains for two very different chain lengths, which resemble short (10-mers) and long (100-mers) chains, at different concentrations of the binary mixture. We clearly show how the concentration variable affects the enrichment of either of the two polymer species at the interface, and also how the chain length influences this process.
Ergodic H-S/MRC Mutual Information

This paper studies the ergodic mutual information of hybrid selection/maximal-ratio combining (H-S/MRC) diversity system under BPSK/QPSK modulations. We consider a simple single-input multiple-output (SIMO) channel, where a subset of branches are selected and combined using maximal-ratio combining (MRC) to maximize the instantaneous Signal to Noise Ratio (SNR) at the receiver. For independent and identically distributed (i.i.d.) Rayleigh flat fading, a general recursive expression is developed to estimate the ergodic input-output mutual information of the whole system. Besides analytical derivations, simulations are provided to demonstrate the feasibility and validity of the derived results.
Effect of Haloperidol on Survival Among Critically Ill Adults With a High Risk of Delirium: The REDUCE Randomized Clinical Trial

Importance Results of studies on use of prophylactic haloperidol in critically ill adults are inconclusive, especially in patients at high risk of delirium. Objective To determine whether prophylactic use of haloperidol improves survival among critically ill adults at high risk of delirium, which was defined as an anticipated intensive care unit (ICU) stay of at least 2 days. Design, Setting, and Participants Randomized, double-blind, placebo-controlled investigator-driven study involving 1789 critically ill adults treated at 21 ICUs, at which nonpharmacological interventions for delirium prevention are routinely used in the Netherlands. Patients without delirium whose expected ICU stay was at least a day were included. Recruitment was from July 2013 to December 2016 and follow-up was conducted at 90 days with the final follow-up on March 1, 2017. Interventions Patients received prophylactic treatment 3 times daily intravenously either 1 mg (n = 350) or 2 mg (n = 732) of haloperidol or placebo (n = 707), consisting of 0.9% sodium chloride. Main Outcome and Measures The primary outcome was the number of days that patients survived in 28 days. There were 15 secondary outcomes, including delirium incidence, 28-day delirium-free and coma-free days, duration of mechanical ventilation, and ICU and hospital length of stay. Results All 1789 randomized patients (mean, age 66.6 years [SD, 12.6]; 1099 men [61.4%]) completed the study. The 1-mg haloperidol group was prematurely stopped because of futility. There was no difference in the median days patients survived in 28 days, 28 days in the 2-mg haloperidol group vs 28 days in the placebo group, for a difference of 0 days (95% CI, 0-0; P = .93) and a hazard ratio of 1.003 (95% CI, 0.78-1.30, P=.82). All of the 15 secondary outcomes were not statistically different. These included delirium incidence (mean difference, 1.5%, 95% CI, −3.6% to 6.7%), delirium-free and coma-free days (mean difference, 0 days, 95% CI, 0-0 days), and duration of mechanical ventilation, ICU, and hospital length of stay (mean difference, 0 days, 95% CI, 0-0 days for all 3 measures). The number of reported adverse effects did not differ between groups (2 [0.3%] for the 2-mg haloperidol group vs 1 [0.1%] for the placebo group). Conclusions and Relevance Among critically ill adults at high risk of delirium, the use of prophylactic haloperidol compared with placebo did not improve survival at 28 days. These findings do not support the use of prophylactic haloperidol for reducing mortality in critically ill adults. Trial Registration clinicaltrials.gov Identifier: NCT01785290
OFDM and Its Wireless Applications: A Survey

Orthogonal frequency-division multiplexing (OFDM) effectively mitigates intersymbol interference (ISI) caused by the delay spread of wireless channels. Therefore, it has been used in many wireless systems and adopted by various standards. In this paper, we present a comprehensive survey on OFDM for wireless communications. We address basic OFDM and related modulations, as well as techniques to improve the performance of OFDM for wireless communications, including channel estimation and signal detection, time- and frequency-offset estimation and correction, peak-to-average power ratio reduction, and multiple-input-multiple-output (MIMO) techniques. We also describe the applications of OFDM in current systems and standards.
Genetic and Environmental Analysis of Behavioral Risk Factors for Adolescent Drug Use in a Community Twin Sample

Abstract We investigated the etiology of six problem behaviors that might facilitate an understanding of behavioral pathways to substance use and abuse in adolescents. These behavioral measures, classified as Conduct Problems, Hyperactivity, School Problems, Low Self-esteem, Neuroticism, and Social Withdrawal were the result of a previously reported (Siewert et al., 2003) modification of the Drug Use Screening Inventory (DUSI; Tarter, 1990; Tarter & Hegedus, 1991). We developed these measures as interpretable components of risk for substance use and abuse in a community based sample of 633 twin pairs, who were under the legal drinking age of 21 (mean age = 15.0 years). Using multivariate analyses, model comparisons indicated that these six behavioral measures could be thought of as two heritable, and genetically distinct, dimensions of problem behavior. Two closely competing models resulted from our analyses. The best fitting model hypothesized a general genetic factor loading on all 6 behavioral measures with a second genetic factor loading on only the three internalizing behavioral measures with loadings of 0.25–0.59 and 0.26–0.44, respectively. A second model, which fit the data almost as well, hypothesized one genetic factor loading only on the externalizing behavioral measures, and a second genetic factor loading only on the internalizing behavioral measures, with a correlation between the two latent factors of 0.75. Because our analyses show that there are two genetically distinct factors influencing these six problem behaviors, we anticipate that there may be different patterns of relationship of these factors to risk for substance use, abuse, and dependence.
Semantic Feature Augmentation in Few-shot Learning

A fundamental problem with few-shot learning is the scarcity of data in training. A natural solution to alleviate this scarcity is to augment the existing images for each training class. However, directly augmenting samples in image space may not necessarily, nor sufficiently, explore the intra-class variation. To this end, we propose to directly synthesize instance features by leveraging the semantics of each class. Essentially, a novel auto-encoder network dual TriNet, is proposed for feature augmentation. The encoder TriNet projects multi-layer visual features of deep CNNs into the semantic space. In this space, data augmentation is induced, and the augmented instance representation is projected back into the image feature spaces by the decoder TriNet. Two data argumentation strategies in the semantic space are explored; notably these seemingly simple augmentations in semantic space result in complex augmented feature distributions in the image feature space, resulting in substantially better performance. The code and models of our paper will be published on: this https URL
Is there an association between nurse, clinical teacher and peer feedback for trainee doctors’ medical specialty choice? An observational study in Taiwan

Objectives This study explored whether there is an association between medical trainees’ future specialty choices and the 360-degree feedback they receive. We hypothesised that the higher the scores that teachers, trainees and/or nurses give to postgraduate year 1s (PGY1s) in any given specialty, the more likely that they will choose that specialty for their residency. Setting The study was conducted in a large regional teaching hospital in Taiwan. Participants The participants of this study were n=66 PGY1s who had completed their medical studies domestically or internationally and had received their PGY1 training in a single teaching hospital in southern Taiwan. Data from 990 assessments were included. Primary and secondary outcome measures Logistic regression analyses for teachers’, nursing staff and peers’ authentic assessments of trainees were undertaken for (1) desired specialty, (2) applied specialty, (3) enrolled specialty, (4) consistency between desired and applied specialties, (5) consistency between applied and enrolled specialties and (6) consistency between desired and enrolled specialties. Alpha was set at p<0.05. Results Nursing staff scores were significantly associated with all six dependent variables. Furthermore, teachers’ scores were significantly associated with trainees’ desired specialty and the consistency between desired and enrolled specialty. Peers’ scores were not significantly associated with any dependent variable. Conclusions Trainees’ specialty choices are associated with scores given by nursing staff and clinical teachers. We suggest that qualitative research methods should further explore this association to ascertain whether PGY1s are consciously influenced by these scores and if so, in what way.
Several Experiments on Investigating Pretraining and Knowledge-Enhanced Models for Natural Language Inference

Natural language inference (NLI) is among the most challenging tasks in natural language understanding. Recent work on unsupervised pretraining that leverages unsupervised signals such as language-model and sentence prediction objectives has shown to be very effective on a wide range of NLP problems. It would still be desirable to further understand how it helps NLI; e.g., if it learns artifacts in data annotation or instead learn true inference knowledge. In addition, external knowledge that does not exist in the limited amount of NLI training data may be added to NLI models in two typical ways, e.g., from human-created resources or an unsupervised pretraining paradigm. We runs several experiments here to investigate whether they help NLI in the same way, and if not,how?