Miracle-dz/UniFAR-FTUs huggingface dataset:
Roles of CD34+ cells and ALK5 signaling in the reconstruction of seminiferous tubule-like structures in 3-D re-aggregate culture of dissociated cells from neonatal mouse testes

Tissue reconstruction in vitro can provide, if successful, a refined and simple system to analyze the underlying mechanisms that drive the morphogenesis and maintain the ordered structure. We have recently succeeded in reconstruction of seminiferous cord-like and tubule-like structures using 3-D re-aggregate culture of dissociated testicular cells. In testis formation, endothelial cells that migrated from mesonephroi to embryonic gonads have been shown to be critical for development of testis cords, but how endothelial cells contribute to testis cord formation remains unknown. To decipher the roles of endothelial and peritubular cells in the reconstruction of cord-like and tubule-like structures, we investigated the behavior of CD34+ endothelial and p75+ cells, and peritubular myoid cells (PTMCs) in 3-D re-aggregate cultures of testicular cells. The results showed that these 3 types of cells had the capacity of re-aggregation on their own and with each other, and of segregation into 3 layers in a re-aggregate, which were very similar to interstitial and peritubular tissues in vivo. Observation of behaviors of fluorescent Sertoli cells and other non-fluorescent types of cells using testes from Sox9-EGFP transgenic mice showed dynamic cell movement and segregation in re-aggregate cultures. Cultures of testicular cells deprived of interstitial and peritubular cells resulted in dysmorphic structures, but re-addition of them restored tubule-like structures. Purified CD34+ cells in culture differentiated into p75+ cells and PTMCs. These results indicate that CD34+ cells differentiate into p75+ cells, which then differentiate into PTMCs. TGFβ signaling inhibitors, SB431542 and ALK5i, disturbed the reconstruction of cord-like and tubule-like structures, and the latter compromised re-construction of interstitial-like and peritubular-like structures, as well as the proliferation of CD34+, p75+, PTMCs, and Sertoli cells, and their movement and differentiation. These results indicate that CD34+ cells and signaling through ALK5 play pivotal roles in the morphogenesis of interstitial-like, peritubular-like and cord-like structures.
Talk Matters at Work: The Effects of Leader-Member Conversational Quality and Communication Frequency on Work Role Stressors

Although it is clear that leadership plays a significant role in followers’ psychological health, the specific mechanisms by which leadership effects may take place await further theorizing and investigation. We argue that communication practices may constitute such specific mechanisms. Therefore, the purpose of this study is to examine how leader-member conversational quality (LMCQ) and communication frequency are associated with members’ perception of work role stressors. Through an online survey, the study found that LMCQ has a significant predictive effect on work role ambiguity and role overload. However, LMCQ interacts with communication frequency in their effects on role conflict. These findings contribute to theories of leadership communication and the continuous development of role dynamics theory.
THE MYTH OF AMERICAN SELFHOOD AND EMOTION: Raising a Sociocentric Child among Middle-Class Americans

In this article, I examine the concepts of the self and emotion reflected in American middle-class socialization practices. Detailed ethnographic description of everyday socialization practices in an American middle-class preschool shows that contrary to the characterization that American notions of self and emotion are predominantly individualistic and egocentric, middle-class socialization practices are highly oriented toward developing sociocentric values such as niceness, cooperation, social appropriateness, empathy, friendship, politeness, and manners. I argue that the dichotomous model of self and emotion that consists of only two types—an egocentric Western self and a sociocentric non-Western self—fails to adequately describe variations and complexity in American experiences of self and emotion. The article contributes to a growing body of research that critically discusses the bipolarized model and argues for inherent dynamism and heterogeneity in our conceptions of the self and emotions.
Pacific Subtropical Cell Response to Reduced Equatorial Dissipation

Abstract Equatorial turbulent diffusivities resulting from breaking gravity waves may be more than a factor of 10 less than those in the midlatitudes. A coupled general circulation model with a layered isopycnal coordinate ocean is used to assess Pacific climate sensitivity to a latitudinally varying background diapycnal diffusivity with extremely low values near the equator. The control experiments have a minimum upper-ocean diffusivity of 10−5 m2 s−1 and are initialized from present-day conditions. The average depth of the σθ = 26.4 interface (z26.4) in the Pacific increases by ∼140 m after 500 yr of coupled model integration. This corresponds to a warming trend in the upper ocean. Low equatorial diffusivities reduce the z26.4 bias by ∼30%. Isopycnal surfaces are elevated from the eastern boundary up to midlatitudes by cooling in the upper several hundred meters, partially compensated by freshening. Entrainment of intermediate water masses from below σθ = 26.4 decreases by ∼1.5 Sv (1 Sv ≡ 106 m3 s−1), m.
Altered Expression of Endogenous Soluble Vascular Endothelial Growth Factor Receptor-2 Is Involved in the Progression of Esophageal Squamous Cell Carcinoma

Endogenous soluble vascular endothelial growth factor receptor-2 (esVEGFR-2), a new splicing variant of VEGFR-2, was shown to be the first endogenous specific inhibitor of lymphatic vessel growth. The expression of esVEGFR-2 and its clinicopathological roles in esophageal squamous cell carcinoma (ESCC) are unclear. In this article, quantitative RT-PCR was employed to detect the mRNA levels of esVEGFR-2 and VEGF-C in 90 paired primary ESCC tissues, along with immunohistochemical staining to measure esVEGFR-2 protein in 182 ESCC primary tissues. Correlations between esVEGFR-2 expression and clinicopathological features were also analyzed. Compared with the corresponding non-neoplastic esophageal mucosa tissues, the mRNA level of esVEGFR-2 was decreased, whereas the mRNA level of VEGF-C was increased in ESCCs. Downregulation of esVEGFR-2 mRNA level was significantly correlated with pTNM stages (χ2 = 7.790, p=0.02). Immunohistochemical staining of esVEGFR-2 was inclined to be reduced in ESCC tissues; lower esVEGFR-2 protein expression was related to better prognosis (χ2 = 6.366, p=0.012), whereas higher esVEGFR-2 protein accumulation in ESCC tissues was an independent prognostic factor for poor survival of patients (hazard ratio, 1.606; 95% confidence interval, 1.042–2.476; p=0.032). Taken together, altered expression of esVEGFR-2 is correlated with progression of ESCC. esVEGFR-2 might serve as a new independent prognostic marker for ESCC patients.
The onset of meandering in a barotropic jet

This study explores the dynamics of an unstable jet of two-dimensional, incompressible fluid on the beta-plane. In the inviscid limit, standard weakly nonlinear theory fails to give a low-order description of this problem, partly because the simple shape of the unstable normal mode is insufficient to capture the structure of the forming pattern. That pattern takes the form of ‘cat’s eyes’ in the vorticity distribution which develop inside the modal critical layers (slender regions to either side of the jet’s axis surrounding the levels where the modal wave speed matches the mean flow). Asymptotic expansions furnish a reduced model which is a version of what is known as the single-wave model in plasma physics. The reduced model predicts that the amplitude of the unstable mode saturates at a relatively low level and is not steady. Rather, the amplitude evolves aperiodically about the saturation level, a result with implications for Lagrangian transport theories. The aperiodic amplitude ‘bounces’ are intimately connected with sporadic deformations of the vortices within the cat’s eyes. The theory is compared with numerical simulations of the original governing equations. Slightly asymmetrical jets are also studied. In this case the neutral modes along the stability boundary become singular; an extension of the weakly nonlinear theory is presented for these modes.
Predictors of pressure ulcers in adult critical care patients.

BACKGROUND Pressure ulcers are one of the most underrated conditions in critically ill patients. Despite the introduction of clinical practice guidelines and advances in medical technology, the prevalence of pressure ulcers in hospitalized patients continues to escalate. Currently, consensus is lacking on the most important risk factors for pressure ulcers in critically ill patients, and no risk assessment scale exclusively for pressure ulcers in these patients is available. OBJECTIVE To determine which risk factors are most predictive of pressure ulcers in adult critical care patients. Risk factors investigated included total score on the Braden Scale, mobility, activity, sensory perception, moisture, friction/shear, nutrition, age, blood pressure, length of stay in the intensive care unit, score on the Acute Physiology and Chronic Health Evaluation II, vasopressor administration, and comorbid conditions. METHODS A retrospective, correlational design was used to examine 347 patients admitted to a medical-surgical intensive care unit from October 2008 through May 2009. RESULTS According to direct logistic regression analyses, age, length of stay, mobility, friction/shear, norepinephrine infusion, and cardiovascular disease explained a major part of the variance in pressure ulcers. CONCLUSION Current risk assessment scales for development of pressure ulcers may not include risk factors common in critically ill adults. Development of a risk assessment model for pressure ulcers in these patients is warranted and could be the foundation for development of a risk assessment tool.
The effect of FasL expression on pancreatic islet allografts.

OBJECTIVE To investigate the immune privilege induced by the Fas ligand (FasL) expressed by cotransplanted testicular Sertoli cells in islet allografts, and the effect of FasL gene transfection on islet cells in pancreatic islet allografts. METHODS Allogeneic islets and testicular cells were cotransplanted into diabetic recipients. Pancreatic islets were infected with the recombinant adenovirus, AdV-FasL, and transplanted into diabetic recipients. Allograft survival, islet function, apoptosis of infiltrative lymphocytes in allografts and gene transfected islet allografts were analyzed. RESULTS All animals receiving islet allograft alone returned to a diabetic state in a few days (mean survival time 6.3 +/- 0.6 days). When the quantity of testicular cells cotransplanted with islets increased to 1 x 10(7), all animals remained normoglycemic throughout the follow-up period (60 days). FasL expression by cotransplanted Sertoli cells induced apoptosis of activated lymphocytes. Rejection of allografts in the FasL gene transfer group was accelerated and allograft survival was shortened to 3.4 +/- 0.2 days (P < 0.05). Pancreatic islets infected with AdV-FasL demonstrated positive staining for FasL at 24h after transplantation, with increased intensity at 48h. Apoptosis assays of pancreatic islet allografts at 24h and 48h revealed apoptosis of transfected islets. CONCLUSIONS FasL-expressing testicular Sertoli cells can induce apoptosis of activated lymphocytes. Cotransplantation of testicular cells allows long-term survival of allogeneic islets because of immune privilege, but the direct expression of FasL on islet allografts infected with AdV-FasL accelerates islet rejection via islet apoptosis and granulocyte infiltration.
Overlapping Protein-Encoding Genes in Pseudomonas fluorescens Pf0-1

The annotated genome sequences of prokaryotes seldom include overlapping genes encoded opposite each other by the same stretch of DNA. However, antisense transcription is becoming recognized as a widespread phenomenon in eukaryotes, and examples have been linked to important biological processes. Pseudomonas fluorescens inhabits aquatic and terrestrial environments, and can be regarded as an environmental generalist. The genetic basis for this ecological success is not well understood. In a previous search for soil-induced genes in P. fluorescens Pf0-1, ten antisense genes were discovered. These were termed ‘cryptic’ genes, as they had escaped detection by gene-hunting algorithms, and lacked easily recognizable promoters. In this communication, we designate such genes as ‘non-predicted’ or ‘hidden’. Using reverse transcription PCR, we show that at each of six non-predicted gene loci chosen for study, transcription occurs from both ‘sense’ and ‘antisense’ DNA strands. Further, at least one of these hidden antisense genes, iiv14, encodes a protein, as does the sense transcript, both identified by poly-histidine tags on the C-terminus of the proteins. Mutational and complementation studies showed that this novel antisense gene was important for efficient colonization of soil, and multiple copies in the wildtype host improved the speed of soil colonization. Introduction of a stop codon early in the gene eliminated complementation, further implicating the protein in colonization of soil. We therefore designate iiv14 “cosA”. These data suggest that, as is the case with eukaryotes, some bacterial genomes are more densely coded than currently recognized.
Are PMS meeting the measurement needs of BPM? A literature review

Purpose – The purpose of this paper is to identify the fundamentals of a performance measurement system (PMS), in order to ascertain if they satisfy the measurement requirements of business process management (BPM) by means of a systematic review of the literature.Design/methodology/approach – The paper uses meta‐analysis to systematically review and examine existing BPM and PMS from the business, non‐business and public sectors. A specific methodology using categorization concept was used to select the appropriate articles. In total, 42 relevant articles are selected and later analyzed. A subsequent content analysis of the information obtained is applied to identify the gaps in the current literature.Findings – The growing interest in PMS has produced an extraordinarily large numbers of papers on the topic. This paper found that, by and large, the PMS as advocated by various authors for over 20 years (since 1990) failed to fulfill the measurement requirements of BPM. This is alarming, considering that pa.
Acquisition Integration and Productivity Losses in the Technical Core: Disruption of Inventors in Acquired Companies

Acquisition integration is a pivotal factor in determining whether the objectives of an acquisition are achieved. In this paper, we hypothesize that the productivity of corporate scientists of acquired companies is generally impaired by integration, but that some scientists experience more disruption than others. In particular, acquisition integration will be most disruptive, leading to the most severe productivity drops, for those inventors who have lost the most social status and centrality in the combined entity. Drawing from prior literatures on the knowledge-based view of the firm, and on mergers and acquisitions, we develop hypotheses about a concise set of conditions that will lead to substantial performance drops for acquired technical personnel. We test our hypotheses, using patent application data, on a sample of 3,933 inventors in pharmaceutical firms whose companies were acquired. Results are strongly in line with our theorized expectations.
Evaluation of ethanol's effects on the biophysical characteristics of licking

Alcohol use disorders are a public health issue related to adverse effects for individuals and society. A low level of response, or decreased sensitivity, to alcohol has been identified as a heritable risk factor for development of alcohol use disorders. One method for researching level of response to alcohol is through the use of rodent models, which are developed to mimic human conditions while eliminating barriers to conducting research with people. Current rodent models used to evaluate effects of ethanol on motor performance have been criticized for not being well matched to human tasks that measure level of change in body sway after alcohol consumption. This study looks at oromotor behavior as a potential alternative to gross motor performance in hopes of increasing correspondence between human and rodent measures of intoxication. To evaluate rodent oromotor performance a force transducer lickometer is used to measure several dimensions of licking behavior after administration of different concentrations of ethanol solution via gavage. Results show that force of licking is not sensitive to dose of ethanol. The total number of licks per session show dose related decreases and licking rhythm, evaluated by the length and distribution of interlick intervals, either increased or decreased for three of the four subjects. Recommendations are made for procedural modifications in order to reduce variability in data and further investigate oromotor performance and level of response to alcohol.
Costs Of Using “Tiny Targets” to Control Glossina fuscipes fuscipes, a Vector of Gambiense Sleeping Sickness in Arua District of Uganda

Introduction To evaluate the relative effectiveness of tsetse control methods, their costs need to be analysed alongside their impact on tsetse populations. Very little has been published on the costs of methods specifically targeting human African trypanosomiasis Methodology/Principal Findings In northern Uganda, a 250 km2 field trial was undertaken using small (0.5 X 0.25 m) insecticide-treated targets (“tiny targets”). Detailed cost recording accompanied every phase of the work. Costs were calculated for this operation as if managed by the Ugandan vector control services: removing purely research components of the work and applying local salaries. This calculation assumed that all resources are fully used, with no spare capacity. The full cost of the operation was assessed at USD 85.4 per km2, of which USD 55.7 or 65.2% were field costs, made up of three component activities (target deployment: 34.5%, trap monitoring: 10.6% and target maintenance: 20.1%). The remaining USD 29.7 or 34.8% of the costs were for preliminary studies and administration (tsetse surveys: 6.0%, sensitisation of local populations: 18.6% and office support: 10.2%). Targets accounted for only 12.9% of the total cost, other important cost components were labour (24.1%) and transport (34.6%). Discussion Comparison with the updated cost of historical HAT vector control projects and recent estimates indicates that this work represents a major reduction in cost levels. This is attributed not just to the low unit cost of tiny targets but also to the organisation of delivery, using local labour with bicycles or motorcycles. Sensitivity analyses were undertaken, investigating key prices and assumptions. It is believed that these costs are generalizable to other HAT foci, although in more remote areas, with denser vegetation and fewer people, costs would increase, as would be the case for other tsetse control techniques.
tatic and dynamic light cattering of healthy nd malaria-parasite nvaded red blood cells

ongKeun Park,* Monica Diez-Silva, Dan Fu, abriel Popescu, Wonshik Choi, Ishan Barman, ubra Suresh, and Michael S. Feld Massachusetts Institute of Technology, G. R. Harrison pectroscopy Laboratory, 77 Massachusetts venue, Cambridge, Massachusetts 02139 Massachusetts Institute of Technology, Department of aterials Science and Engineering, 77 Massachusetts venue, Cambridge, Massachusetts 02139 University of Illinois at Urbana-Champaign, Beckman nstitute for Advanced Science and Technology, Department f Electrical and Computer Engineering, 405 North athews Avenue, Urbana, Illinois 61801 Korea University, Department of Physics, Anam-dong ungbuk-gu, Seoul, 136-701 Korea Massachusetts Institute of Technology, School of ngineering, 77 Massachusetts Avenue, Cambridge, assachusetts 02139
Differentiating Osteoarticular Infections Caused By Kingella Kingae From Those Due to Typical Pathogens in Young Children

Osteoarticular infections caused by Kingella kingae are characterized by mild-to-moderate clinical and biologic inflammatory signs that are different from those caused by Gram-positive cocci. A combined score was built to find the best model to predict K. kingae osteoarticular infections by using the following 4 variables: body temperature <38°C, serum C-reactive protein <55 mg/L, white blood cell count <14,000/mm3, and band forms <150/mm3.
General logarithmic corrections to black-hole entropy

We compute leading-order corrections to the entropy of any thermodynamic system due to small statistical fluctuations around equilibrium. When applied to black holes, these corrections are shown to be of the form −k ln(Area). For BTZ black holes, k = 3/2, as found earlier. We extend the result to anti-de Sitter Schwarzschild and Reissner–Nordstrom black holes in arbitrary dimensions. Finally we examine the role of conformal field theory in black-hole entropy and its corrections.
Antimicrobial Protein and Peptide Concentrations and Activity in Human Breast Milk Consumed by Preterm Infants at Risk of Late-Onset Neonatal Sepsis

Objective We investigated the levels and antimicrobial activity of antimicrobial proteins and peptides (AMPs) in breast milk consumed by preterm infants, and whether deficiencies of these factors were associated with late-onset neonatal sepsis (LOS), a bacterial infection that frequently occurs in preterm infants in the neonatal period. Study design Breast milk from mothers of preterm infants (≤32 weeks gestation) was collected on days 7 (n = 88) and 21 (n = 77) postpartum. Concentrations of lactoferrin, LL-37, beta-defensins 1 and 2, and alpha-defensin 5 were measured by enzyme-linked immunosorbent assay. The antimicrobial activity of breast milk samples against Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae was compared to the activity of infant formula, alone or supplemented with physiological levels of AMPs. Samples of breast milk fed to infants with and without subsequent LOS were compared for levels of AMPs and inhibition of bacterial growth. Results Levels of most AMPs and antibacterial activity in preterm breast milk were higher at day 7 than at day 21. Lactoferrin was the only AMP that limited pathogen growth >50% when added to formula at a concentration equivalent to that present in breast milk. Levels of AMPs were similar in the breast milk fed to infants with and without LOS, however, infants who developed LOS consumed significantly less breast milk and lower doses of milk AMPs than those who were free from LOS. Conclusions The concentrations of lactoferrin and defensins in preterm breast milk have antimicrobial activity against common neonatal pathogens.