Miracle-dz/UniFAR-FTUs huggingface dataset:
New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp. Dairy Isolates

Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faecium (15), En. faecalis (6), En. italicus (3), and En. hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products' microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coli and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.
Evaluation of the Effects of the Occlu-Pad for the Management of Anisometropic Amblyopia in Children

ABSTRACT Purpose: In recent years, amblyopia treatment device that can be used with both eyes open have been reported. The Occlu-pad is a device that can present images of tablet terminals to one eye only under binocular open conditions. Till date, no study has reported the effectiveness of Occlu-pad training for anisometropic amblyopia in a series of cases. In the present study, we evaluated the effectiveness of the Occlu-pad for the management of anisometropic amblyopia without the use of occlusion therapy (eyepatch). Materials and Methods: We implemented Occlu-pad training for 22 children (mean age ± standard deviation: 4.7 ± 1.2 years) with anisometropic amblyopia. The visual acuity before treatment initiation was 0.25 ± 0.08. The difference in refraction between the healthy and amblyopic eyes was 3.10 ± 0.58 D. Results: The visual acuity at 3 months and 6 months after training initiation was 0.06 ± 0.09 and −0.04 ± 0.07, respectively; this indicates a significant improvement in vision. The compliance rates for Occlu-pad use during 0 − 3 months and 4 − 6 months after training initiation were 88.6% ± 18.9% and 73.2% ± 18.9%; these rates decreased significantly with time. Conclusions: Our findings suggest that the Occlu-pad is an effective tool for the management of anisometropic amblyopia in children.
Pan-Asian adapted ESMO consensus guidelines for the management of patients with metastatic colorectal cancer: a JSMO–ESMO initiative endorsed by CSCO, KACO, MOS, SSO and TOS

The most recent version of the European Society for Medical Oncology (ESMO) consensus guidelines for the treatment of patients with metastatic colorectal cancer (mCRC) was published in 2016, identifying both a more strategic approach to the administration of the available systemic therapy choices, and a greater emphasis on the use of ablative techniques, including surgery. At the 2016 ESMO Asia Meeting, in December 2016, it was decided by both ESMO and the Japanese Society of Medical Oncology (JSMO) to convene a special guidelines meeting, endorsed by both ESMO and JSMO, immediately after the JSMO 2017 Annual Meeting. The aim was to adapt the ESMO consensus guidelines to take into account the ethnic differences relating to the toxicity as well as other aspects of certain systemic treatments in patients of Asian ethnicity. These guidelines represent the consensus opinions reached by experts in the treatment of patients with mCRC identified by the Presidents of the oncological societies of Japan (JSMO), China (Chinese Society of Clinical Oncology), Korea (Korean Association for Clinical Oncology), Malaysia (Malaysian Oncological Society), Singapore (Singapore Society of Oncology) and Taiwan (Taiwan Oncology Society). The voting was based on scientific evidence and was independent of both the current treatment practices and the drug availability and reimbursement situations in the individual participating Asian countries.
Criteria Comparison for Classifying Peatland Vegetation Types Using In Situ Hyperspectral Measurements

This study aims to evaluate three classes of methods to discriminate between 13 peatland vegetation types using reflectance data. These vegetation types were empirically defined according to their composition, strata and biodiversity richness. On one hand, it is assumed that the same vegetation type spectral signatures have similarities. Consequently, they can be compared to a reference spectral database. To catch those similarities, several similarities criteria (related to distances (Euclidean distance, Manhattan distance, Canberra distance) or spectral shapes (Spectral Angle Mapper) or probabilistic behaviour (Spectral Information Divergence)) and several mathematical transformations of spectral signatures enhancing absorption features (such as the first derivative or the second derivative, the normalized spectral signature, the continuum removal, the continuum removal derivative reflectance, the log transformation) were investigated. Furthermore, those similarity measures were applied on spectral ranges which characterize specific biophysical properties. On the other hand, we suppose that specific biophysical properties/components may help to discriminate between vegetation types applying supervised classification such as Random Forest (RF), Support Vector Machines (SVM), Regularized Logistic Regression (RLR), Partial Least Squares-Discriminant Analysis (PLS-DA). Biophysical components can be used in a local way considering vegetation spectral indices or in a global way considering spectral ranges and transformed spectral signatures, as explained above. RLR classifier applied on spectral vegetation indices (training size = 25%) was able to achieve 77.21% overall accuracy in discriminating peatland vegetation types. It was also able to discriminate between 83.95% vegetation types considering specific spectral range [[range-phrase = –]3501350 nm ], first derivative of spectral signatures and training size = 25%. Conversely, similarity criterion was able to achieve 81.70% overall accuracy using the Canberra distance computed on the full spectral range [[range-phrase = –]3502500 nm ]. The results of this study suggest that RLR classifier and similarity criteria are promising to map the different vegetation types with high ecological values despite vegetation heterogeneity and mixture.
The Integration of Realistic Episodic Memories Relies on Different Working Memory Processes: Evidence from Virtual Navigation

Memory is one of the most important cognitive functions in a person’s life as it is essential for recalling personal memories and performing many everyday tasks. Although a huge number of studies have been conducted in the field, only a few of them investigated memory in realistic situations, due to methodological issues. The various tools that have been developed using virtual environments (VEs) have gained popularity in cognitive psychology and neuropsychology because they enable to create naturalistic and controlled situations, and are thus particularly adapted to the study of episodic memory (EM), for which an ecological evaluation is of prime importance. EM is the conscious recollection of personal events combined with their phenomenological and spatiotemporal encoding contexts. Using an original paradigm in a VE, the objective of the present study was to characterize the construction of episodic memories. While the concept of working memory has become central in the understanding of a wide range of cognitive functions, its role in the integration of episodic memories has seldom been assessed in an ecological context. This experiment aimed at filling this gap by studying how EM is affected by concurrent tasks requiring working memory resources in a realistic situation. Participants navigated in a virtual town and had to memorize as many elements in their spatiotemporal context as they could. During learning, participants had either to perform a concurrent task meant to prevent maintenance through the phonological loop, or a task aimed at preventing maintenance through the visuospatial sketchpad, or no concurrent task. EM was assessed in a recall test performed after learning through various scores measuring the what, where and when of the memories. Results showed that, compared to the control condition with no concurrent task, the prevention of maintenance through the phonological loop had a deleterious impact only on the encoding of central elements. By contrast, the prevention of visuo-spatial maintenance interfered both with the encoding of the temporal context and with the binding. These results suggest that the integration of realistic episodic memories relies on different working memory processes that depend on the nature of the traces.
Strength of Aramid-Epoxy Composites during Moisture Absorption

Tests were performed to determine the effects of a fiber surface treatment on the interlaminar shear strength of dry and moist Twaron aramid-epoxy composites. Unidirectional specimens, some containing treated fibers and some containing untreated fibers, were subjected to elevated temperatures and humidities for periods of up to 14 months. Periodically, the interlaminar shear strength was measured and correlated with the moisture concentration at the plane of failure, i.e., near the midplane of the specimen. The data indicated that for dry specimens, the fiber surface treatment increased the in terlaminar shear strength by about 44% over specimens with untreated fibers. As the spec imens absorbed moisture, their strengths decreased, in inverse proportion to the moisture concentration at the plane of failure. For a given moisture concentration at the plane of failure, the specimens with treated fibers were stronger than those with untreated fibers. However this difference in strength decreased with increasing moisture concentrations.
Self-sensing of temperature rises on light emitting diode based optrodes

Objective. This work presents a method to determine the surface temperature of microphotonic medical implants like LEDs. Our inventive step is to use the photonic emitter (LED) employed in an implantable device as its own sensor and develop readout circuitry to accurately determine the surface temperature of the device. Approach. There are two primary classes of applications where microphotonics could be used in implantable devices; opto-electrophysiology and fluorescence sensing. In such scenarios, intense light needs to be delivered to the target. As blue wavelengths are scattered strongly in tissue, such delivery needs to be either via optic fibres, two-photon approaches or through local emitters. In the latter case, as light emitters generate heat, there is a potential for probe surfaces to exceed the 2 °C regulatory. However, currently, there are no convenient mechanisms to monitor this in situ. Main results. We present the electronic control circuit and calibration method to monitor the surface temperature change of implantable optrode. The efficacy is demonstrated in air, saline, and brain. Significance. This paper, therefore, presents a method to utilize the light emitting diode as its own temperature sensor.
Modelling vocabulary development among multilingual children prior to and following the transition to school entry

ABSTRACT Differences between monolingual and multilingual vocabulary development have been observed but few studies provide a longitudinal perspective on vocabulary development before and following school entry. This study compares vocabulary growth profiles of 106 multilingual children to 211 monolingual peers before and after school entry to examine whether: (1) school entry coincides with different rates of vocabulary growth compared to prior to school entry, (2) compared to monolingual peers, multilingual children show different vocabulary sizes or rates of vocabulary growth, (3) the age of onset of second-language acquisition for multilingual children is associated with vocabulary size or rate of vocabulary growth, and (4) the sociolinguistic context of the languages spoken by multilingual children is associated with vocabulary size or rate of vocabulary growth. Results showed increases in vocabulary size across time for all children, with a steeper increase prior to school entry. A significant difference between monolingual and multilingual children who speak a minority language was observed with regards to vocabulary size at school entry and vocabulary growth prior to school entry, but growth rate differences were no longer present following school entry. Taken together, results suggest that which languages children speak may matter more than being multilingual per se.
Greedy Algorithms for Cone Constrained Optimization with Convergence Guarantees

Greedy optimization methods such as Matching Pursuit (MP) and Frank-Wolfe (FW) algorithms regained popularity in recent years due to their simplicity, effectiveness and theoretical guarantees. MP and FW address optimization over the linear span and the convex hull of a set of atoms, respectively. In this paper, we consider the intermediate case of optimization over the convex cone, parametrized as the conic hull of a generic atom set, leading to the first principled definitions of non-negative MP algorithms for which we give explicit convergence rates and demonstrate excellent empirical performance. In particular, we derive sublinear ($\mathcal{O}(1/t)$) convergence on general smooth and convex objectives, and linear convergence ($\mathcal{O}(e^{-t})$) on strongly convex objectives, in both cases for general sets of atoms. Furthermore, we establish a clear correspondence of our algorithms to known algorithms from the MP and FW literature. Our novel algorithms and analyses target general atom sets and general objective functions, and hence are directly applicable to a large variety of learning settings.
Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol‐Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns

Harvesting of autografts results in donor site morbidities and is limited in scenarios such as large total body surface area burns. In these instances, coverage is increased by meshing grafts at the expense of delayed biologic closure. Moreover, graft meshing increases the likelihood of contraction and hypertrophic scarring, limits range of motion, and worsens cosmesis. Many tissue engineering technologies have touted the promise of adipose‐derived stem cells (ASCs) for burn wounds. The primary objective of the current study was to determine feasibility and efficacy of in situ ASC delivery via PEGylated fibrin (FPEG) hydrogels as adjuncts to meshed split thickness skin grafts in a porcine model. Deep partial thickness burns were created on the dorsum of anesthetized Yorkshire pigs, and subsequently debrided on post‐burn day 4. After debridement, wounds were treated with: split thickness skin grafts (STSG); meshed STSG (mSTSG); and mSTSG + FPEG with increasing doses of ASCs. We show that FPEG hydrogels can be delivered in situ to prevent the contraction seen after meshing of STSG. Moreover, ASCs delivered in FPEG dose‐dependently increase blood vessel size which significantly correlates with CD31 protein levels. The current study reports a dual‐action adjunct therapy to autografting administered in situ, wherein FPEG acts as both scaffolding to prevent contraction, and as a delivery vehicle for ASCs to accelerate angiogenesis. This strategy may be used to incorporate other biologics for generating tissue engineered products aimed at improving wound healing and minimizing donor sites or scarring. Stem Cells Translational Medicine 2018;7:360–372
Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication

Positive-sense (+) RNA viruses represent the most abundant group of viruses and are dependent on the host cell machinery to replicate. One remarkable feature that occurs after (+) RNA virus entry into cells is the remodeling of host endomembranes, leading to the formation of viral replication factories. Recently, rapid progress in three-dimensional (3D) imaging technologies, such as electron tomography (ET) and focused ion beam-scanning electron microscopy (FIB-SEM), has enabled researchers to visualize the novel membrane structures induced by viruses at high resolution. These 3D imaging technologies provide new mechanistic insights into the viral infection cycle. In this review, we summarize the latest reports on the cellular remodeling that occurs during plant virus infection; in particular, we focus on studies that provide 3D architectural information on viral replication factories. We also outline the mechanisms underlying the formation of these membranous structures and discuss possible future research directions.
Factors associated with elevated blood pressure or hypertension in Afro-Caribbean youth: a cross-sectional study

Background Although several studies have identified risk factors for high blood pressure (BP), data from Afro-Caribbean populations are limited. Additionally, less is known about how putative risk factors operate in young adults and how social factors influence the risk of high BP. In this study, we estimated the relative risk for elevated BP or hypertension (EBP/HTN), defined as BP ≥ 120/80 mmHg, among young adults with putative cardiovascular disease (CVD) risk factors in Jamaica and evaluated whether relative risks differed by sex. Methods Data from 898 young adults, 18–20 years old, were analysed. BP was measured with a mercury sphygmomanometer after participants had been seated for 5 min. Anthropometric measurements were obtained, and glucose, lipids and insulin measured from a fasting venous blood sample. Data on socioeconomic status (SES) were obtained via questionnaire. CVD risk factor status was defined using standard cut-points or the upper quintile of the distribution where the numbers meeting standard cut-points were small. Relative risks were estimated using odds ratios (OR) from logistic regression models. Results Prevalence of EBP/HTN was 30% among males and 13% among females (p < 0.001 for sex difference). There was evidence for sex interaction in the relationship between EBP/HTN and some of risk factors (obesity and household possessions), therefore we report sex-specific analyses. In multivariable logistic regression models, factors independently associated with EBP/HTN among men were obesity (OR 8.48, 95% CI [2.64–27.2], p < 0.001), and high glucose (OR 2.01, CI [1.20–3.37], p = 0.008), while high HOMA-IR did not achieve statistical significance (OR 2.08, CI [0.94–4.58], p = 0.069). In similar models for women, high triglycerides (OR 1.98, CI [1.03–3.81], p = 0.040) and high HOMA-IR (OR 2.07, CI [1.03–4.12], p = 0.039) were positively associated with EBP/HTN. Lower SES was also associated with higher odds for EBP/HTN (OR 4.63, CI [1.31–16.4], p = 0.017, for moderate vs. high household possessions; OR 2.61, CI [0.70–9.77], p = 0.154 for low vs. high household possessions). Alcohol consumption was associated with lower odds of EBP/HTN among females only; OR 0.41 (CI [0.18–0.90], p = 0.026) for drinking <1 time per week vs. never drinkers, and OR 0.28 (CI [0.11–0.76], p = 0.012) for drinking ≥3 times per week vs. never drinkers. Physical activity was inversely associated with EBP/HTN in both males and females. Conclusion Factors associated with EBP/HTN among Jamaican young adults include obesity, high glucose, high triglycerides and high HOMA-IR, with some significant differences by sex. Among women lower SES was positively associated with EBP/HTN, while moderate alcohol consumption was associated lower odds of EBP/HTN.
Concurrent eosinophilia and IgG4-related disease in a child: A case report and review of the literature

The current study presents the case of a 9-year-old Chinese boy who presented with eosinophilia and elevated serum levels of immunoglobulin G4 (IgG4). A bone marrow puncture identified an elevated eosinophil rate of 23% (normal range, <5%), which indicated eosinophilia. However, gene analysis, fluorescent in situ hybridization and other examinations, including bone marrow aspiration, blood routine, auto-antibody tests and parasitic and allergens screening, contradicted a diagnosis of secondary or clonal eosinophilia. Furthermore, the patient exhibited multiple lymph node swelling and a lymph biopsy strongly indicted a pathological diagnosis of IgG4-related disease (IgG4-RD). His peripheral blood flow cytometry confirmed an elevated count of plasmablasts, which is specific to IgG4-RD. The patient responded well to therapy with prednisone and remained healthy in all follow-ups. By taking all these factors into consideration, the boy was diagnosed with IgG4-RD. It is difficult to distinguish IgG4-RD from hypereosinophilic syndrome and the potential association between the two remains unclear. However, the present case study serves as a reminder that IgG4-RD may occur in children and medical professionals should not neglect this possibility.
Expansion Analysis of Yangtze River Delta Urban Agglomeration Using DMSP/OLS Nighttime Light Imagery for 1993 to 2012

Investigating the characteristics of urban expansion is helpful in managing the relationship between urbanization and the ecological and environmental issues related to sustainable development. The Defense Meteorological Satellite Program/Operational Line-scan System (DMSP/OLS) collects visible and near-infrared light from the Earth’s surface at night without moonlight. It generates effective time series data for mapping the dynamics of urban expansion. As a major urban agglomeration in the world, the Yangtze River Delta Urban Agglomeration (YRDUA) is an important intersection zone of both the “Belt and Road Initiative” and the “Yangtze River Economic Belt” in China. Therefore, this paper analyses urban expansion characteristics of the YRDUA for 1993–2012 from urban extents extracted from the DMSP/OLS for 1993, 1997, 2002, 2007, and 2012. First, calibration procedures are applied to DMSP/OLS data, including intercalibration, intra-annual composition, and inter-annual series correction procedures. Spatial extents are then extracted from the corrected DMSP/OLS data, and a threshold is determined via the spatial comparison method. Finally, three models are used to explore urban expansion characteristics of the YRDUA from expansion rates, expansion spatial patterns, and expansion evaluations. The results show that the urban expansion of the YRDUA occurred at an increasing rate from 1993–2007 and then declined after 2007 with the onset of the global financial crisis. The Suxichang and Ningbo metropolitan circles were seriously affected by the financial crisis, while the Hefei metropolitan circle was not. The urban expansion of the YRDUA moved from the northeast to the southwest over the 20-year period. Urban expansion involved internal infilling over the first 15 years and then evolved into external sprawl and suburbanization after 2007.