Miracle-dz/UniFAR-FTUs huggingface dataset:
Increased Leptin Response and Inhibition of Apoptosis in Thymocytes of Young Rats Offspring from Protein Deprived Dams during Lactation

We investigated the consequences of mild maternal malnutrition in rat dams, in terms of thymocyte responses and the putative role of leptin. The young progeny of dams submitted to protein deprivation (PD) during lactation showed at 30 days of age lower body and thymus weights, significant alterations in CD4/CD8-defined T cell subsets without modifications in total thymocyte number as well as in proliferative response. Despite, the rats from PD group did not present alterations in leptin circulating levels, the expression of leptin receptor ObRb was enhanced in their thymocytes. This change was accompanied by an increase in leptin signaling response of thymocytes from PD rats, with an increase in JAK2 and STAT3 phosphorylation after leptin stimulation. Thymocytes from PD rats also presented a decreased rate of spontaneous apoptosis when compared to controls. Accordingly, higher expression of anti-apoptotic protein Bcl-2, and lower of pro-apoptotic protein Bax, with no change of pro-apoptotic Bad, and higher pro-caspase 3 content were detected in PD thymocytes. Moreover, thymocytes from PD group exhibited a constitutive higher nuclear content of p65 NF-kB associated to a lower IkB content in the cytoplasm. Finally, although there was no change in ob gene expression in PD thymocytes, a higher mRNA expression for the Ob gene was observed in the thymic microenvironment from PD animals. Taken together, the results show that mild maternal protein deprivation during lactation affects thymic homeostasis, enhancing leptin activity, which in turn protects thymocytes from apoptosis in the young progeny, with possible consequences upon the immune response of these animals in adult life.
Particulate Matter Fluxes in a Mediterranean Mountain Forest: Interspecific Differences Between Throughfall and Stemflow in Oak and Pine Stands

In forested areas, canopies play an important role in the partitioning of rainfall. During this process there is also a redistribution of particulate matter (PM) that is deposited from the atmosphere on vegetative surfaces and transported to soil layers by throughfall and stemflow. We collected samples of rainfall, throughfall, and stemflow from two different forest plots (pine and oak) in a Mediterranean mountainous area and analyzed the amount and size distributions of PM (0.45 μm < PM < 500 μm). The exploration of backward trajectories revealed that PM content varied significantly. This depended on the origin of the air mass, with Atlantic fronts transporting less PM in the atmosphere than North African dust intrusions, which added disproportionate inputs of PM. Overall, throughfall provided the largest proportion of incoming PM under trees, but, at the base of each tree, stemflow led to a localized input of water that was more PM enriched than water through open precipitation or throughfall. Interspecific differences in PM fluxes were noted with pines retaining more PM in their crowns than oaks. Furthermore, the presence of leaves on oak increased the size and the amount of particulates released by throughfall. The PM in stemflow was smaller and rounder than in throughfall. This study adds to our understanding knowledge of the processes that control the deposition and distribution of PM delivered to forest soils, a fraction that is often ignored in studies of nutrient and energy fluxes in ecosystems.
Assessing the micronutrient and macronutrient intakes in female students and comparing them with the set standard values

Background: Healthy nutrition particularly the energy intake and the essential nutrients in female students is very important. This study aims to assess micro- and macronutrient intakes in female students of Isfahan University of Medical Sciences, Iran. Materials and Methods: This cross-sectional study involved 100 female students aged between 18 and 25 years in 2008–2009. Anthropometrics measures were performed and two 24-hours food recalls were used to collect the dietary information and were analyzed using food processor 2 and compared with the dietary reference intakes (DRIs) 2008. Findings: As many as 61.1% of subjects resided in dormitories; 12.7% were married. Prevalence of overweight or obesity and abdominal obesity in the subjects studied were 6.9% and 46.1%, respectively. The mean (±SD) systolic blood pressure was 105.2 ± 15.6 mmHg and the diastolic was 62.2 ± 10.4 mmHg. Totally, 3.9% of the subjects had hypertension. Food intake analysis indicated that B12, folate, magnesium, potassium, and calcium were below the recommended level, and vitamin C, E, pantothenic acid, B1, B3, phosphate, and zinc were above, and energy intake, macronutrient, vitamin A, pyridoxine, iron, and selenium were, in general, adequate. Conclusion: The findings of the study indicated that macronutrients intake was appropriate, but the problem mainly existed in the consumption of micronutrients. It is recommended to increase the intakes of important food groups such as dairy, vegetable, and fruit that are proper sources of micronutrients, and it is also suggested to improve strategies and the competence in this area of nutrition.
Numeracy and Health Numeracy Among Chinese and Kenyan Immigrants to Canada

Mathematics self-efficacy plays an important role in the performance of quantitative tasks. The objective of this study was to examine the role of mathematics self-efficacy in numeracy and health numeracy among immigrants (60 Mandarin-speaking and 60 Kikuyu-speaking immigrants to Canada) for whom English was a second language (ESL). Two French Kit numeracy tasks (the addition, and the addition and subtraction correction tasks) constituted objective measures of numeracy, and the numeracy component of a health literacy instrument (The Short Test of Functional Health Literacy for Adults [S-TOFHLA]) constituted the health numeracy measure. We measured math self-efficacy using the Mathematics Self-Efficacy Scale (MSES) and the Subjective Numeracy Scale (SNS). All measures were presented in English. Kikuyu speakers had lower math self-efficacy (p < .05) compared with Mandarin speakers (p < .05). Mandarin speakers outperformed Kikuyu speakers in numeracy (p < .001) and health numeracy (p < .023) skills. In multiple regression analyses, 32% to 57 % of the variance in objective numeracy was explained by language, residency in Canada, and self-efficacy, adjusting for the other predictor variables; self-efficacy and format of numeric health information (numbers only vs. numbers with detailed text) consistently predicted health numeracy. Mathematics self-efficacy may be an important factor in numeracy, but not as important in health numeracy for Kikuyu- and Mandarin-speaking immigrants for whom English is a second language. Factors contributing to low numeracy may differ from those explaining low health numeracy in ESL immigrants.
Attitude and CAS use in senior secondary mathematics: A case study of seven Year 11 students

This paper investigates the possible influence of attitude on seven Year 11 students’ use of a Computer Algebra System (CAS) during a class activity where students could choose to use CAS or pen-and-paper in solving a range of problems. Investigation of anxiety, confidence, liking and usefulness through a survey and interview revealed that these components of attitude could influence whether or not a student chose to use CAS. One student, who had a positive attitude towards CAS, made extensive use of CAS in solving given problems. This suggested that, in this case, the student’s positive attitude towards CAS may have positively influenced her use of CAS. The potential impact of attitude on CAS use has implications for students studying in a school system where CAS is expected to be used in mathematics.
One‐year results of maxillary overdentures supported by 2 titanium–zirconium implants – implant survival rates and radiographic outcomes

OBJECTIVE To assess implant survival rates and peri-implant bone loss of 2 titanium-zirconium implants supporting maxillary overdentures at 1 year of loading. MATERIAL AND METHODS Twenty maxillary edentulous patients (5 women and 15 men) being dissatisfied with their complete dentures were included. In total, 40 diameter-reduced titanium-zirconium implants were placed in the anterior maxilla. Local guided bone regeneration (GBR) was allowed if the treatment did not compromise implant stability. Following 3 to 5 months of healing, implant-supported overdentures were inserted on two ball anchors. Implants and overdentures were assessed at 1, 2, 4, and 8 weeks after implant insertion and 2, 4, and 12 months after insertion of overdentures (baseline). Standardized radiographs were taken at implant loading and 1 year. Implant survival rates and bone loss were the primary outcomes. RESULTS Nineteen patients (1 dropout) with 38 implants were evaluated at a mean follow-up of 1.1 years (range 1.0-1.7 years). One implant failed resulting in an implant survival rate of 97.3%. There was a significant peri-implant bone loss of the implants at 1 year of function (mean, 0.7 mm, SD = 1.1 mm; median: 0.48 mm, IQR = 0.56 mm). CONCLUSIONS There was a high 1-year implant survival rate for edentulous patients receiving 2 maxillary implants and ball anchors as overdenture support. However, several implants exhibited an increased amount of bone loss of more than 2 mm. Overdentures supported by 2 maxillary implants should thus be used with caution as minimally invasive treatment for specific patients encountering problems with their upper dentures until more long-term data is available.
Aqueous cytokine levels are associated with reduced macular thickness after intravitreal ranibizumab for diabetic macular edema

Purpose It is controversial whether the administration of anti-vascular endothelial growth factor drugs for diabetic macular edema (DME) affects intraocular inflammatory cytokines. In this study, we measured cytokine concentration in aqueous humor before and after intravitreal injection of ranibizumab (IVR). The aim was to determine changes in cytokine concentration and their effects on DME reduction. Methods Twelve patients (13 eyes) with DME received two IVR (0.5 mg) with a 1 month interval, and a total of 26 aqueous humor samples were obtained. Macular thickness was measured with an optical coherence tomography (OCT) using thickness-map mode with an Early Treatment Diabetic Retinopathy Study (ETDRS) 9-zone grid that was divided into two zones: a central circle with a diameter of 1 mm (zone1); and an outer circle with a diameter of 6 mm (zone2). Results The concentration of eotaxin-1 in aqueous humor samples decreased significantly after IVR. Baseline cytokine concentration was associated with IVR-induced DME reduction. In zone1, higher baseline concentration of interferon-induced protein (IP)-10, and in zone 2, higher baseline concentration of granulocyte-macrophage colony-stimulating factor, IP-10, and tumor necrosis factor (TNF) α; and lower baseline concentration of eotaxin-1, interleukin (IL)-5, and IL-8 were associated with improved DME. Cytokine changes were associated with IVR-induced DME reduction. In zone1, lower concentration of IP-10 compared to baseline or higher concentration of macrophage inflammatory protein (MIP) -α, and in zone 2, lower concentration of IL-5 compared to baseline, IL-8, and IP-10 or higher concentration of eotaxin-1 and MIP-1β were associated with improved DME. Conclusions These findings suggest that ranibizumab affects the concentration of cytokines in aqueous humor. Various cytokines contribute to a decrease in retinal thickness, both in the center of the macula and in a larger area of the retina.
Free will in everyday life: Autobiographical accounts of free and unfree actions

What does free will mean to laypersons? The present investigation sought to address this question by identifying how laypersons distinguish between free and unfree actions. We elicited autobiographical narratives in which participants described either free or unfree actions, and the narratives were subsequently subjected to impartial analysis. Results indicate that free actions were associated with reaching goals, high levels of conscious thought and deliberation, positive outcomes, and moral behavior (among other things). These findings suggest that lay conceptions of free will fit well with the view that free will is a form of action control.
Moral Politics: The Religious Factor in Referenda Voting

Abstract This article combines the research strands of moral politics and political behavior by focusing on the effect of individual and contextual religiosity on individual vote decisions in popular initiatives and public referenda concerning morally charged issues. We rely on a total of 13 surveys with 1,000 respondents each conducted after every referendum on moral policies in Switzerland between 1992 and 2012. Results based on cross-classified multilevel models show that religious behaving instead of nominal religious belonging plays a crucial role in decision making on moral issues. This supports the idea that the traditional confessional cleavage is replaced by a new religious cleavage that divides the religious from the secular. This newer cleavage is characterized by party alignments that extend from electoral to direct democratic voting behavior. Overall, our study lends support to previous findings drawn from American research on moral politics, direct democracies, and the public role of religion.
A personal view of how ruminant animals control their intake and choice of food: minimal total discomfort

Voluntary food intake and the selection between foods are important subjects especially in ruminants in view of the economic importance of this class of animal and the complex digestive system with its attendant metabolic peculiarities. There is evidence that intake is limited by the capacity of the rumen as well as by metabolic factors; some theories assume that intake is controlled by the first limiting factor but this is not satisfying on physiological grounds and there is evidence that signals from feedback factors are integrated in an additive manner. It is now well established from research in which animals are given the chance to learn the metabolic consequences of eating food with a particular sensory profile, including a choice of foods, that animals including ruminants can adjust their diet, both quantitatively and qualitatively, to their nutrient requirements. It is proposed that they do this in order to minimise the total of the discomfort generated by the several signals from various body systems. The learning process is aided by the considerable day-to-day variation often seen in the intake of individual animals. An optimisation model is proposed and presented in a simple form, involving the addition of discomforts (calculated as the square of the deviation of the supply of metabolisable energy, crude protein and neutral-detergent fibre) and iterative elucidation of the intake at which total discomfort is minimal. With parameters appropriate for growing lambs the model provides reasonable agreement with observations, both in terms of daily intake and selection between foods of different protein contents. Manipulation of food composition and of nutrient requirements produces predictions broadly in agreement with reality except that protein deficiency has less severe consequences for the model than for real animals; it is proposed that protein deficiency be given more weighting than protein excess, and this may be true for other resources as well. This model is proposed as a philosophy and a starting point for further development and is not purveyed as a complete, working model. It nevertheless provides support for the concept of total minimal discomfort as a suitable base from which to view the control of intake and selection in all animals.
Robust Event-Triggered MPC With Guaranteed Asymptotic Bound and Average Sampling Rate

We propose a robust event-triggered model predictive control (MPC) scheme for linear time-invariant discrete-time systems subject to bounded additive stochastic disturbances and hard constraints on the input and state. For given probability distributions of the disturbances acting on the system, we design event conditions such that the average frequency of communication between the controller and the actuator in the closed-loop system attains a given value. We employ Tube MPC methods to guarantee robust constraint satisfaction and a robust asymptotic bound on the system state. Moreover, we show that instead of a given periodically updated Tube MPC scheme, an appropriate event-triggered MPC scheme can be applied, with the same guarantees on constraints and region of attraction, but with a reduced number of average communications.
Anisotropic stretch-induced hypertrophy in neonatal ventricular myocytes micropatterned on deformable elastomers.

Because cell shape and alignment, cell-matrix adhesion, and cell-cell contact can all affect growth, and because mechanical strains in vivo are multiaxial and anisotropic, we developed an in vitro system for engineering aligned, rod-shaped, neonatal cardiac myocyte cultures. Photolithographic and microfluidic techniques were used to micropattern extracellular matrices in parallel lines on deformable silicone elastomers. Confluent, elongated, aligned myocytes were produced by varying the micropattern line width and collagen density. An elliptical cell stretcher applied 2:1 anisotropic strain statically to the elastic substrate, with the axis of greatest stretch (10%) either parallel or transverse to the myofibrils. After 24 h, the principal strain parallel to myocytes did not significantly alter myofibril accumulation or expression of atrial natriuretic factor (ANF), connexin-43 (Cx-43), or N-cadherin (by indirect immunofluorescent antibody labeling and immunoblotting) compared with unstretched controls. In contrast, 10% transverse principal strain resulted in continuous staining of actin filaments (rhodamine phalloidin); increased immunofluorescent labeling of ANF, Cx-43, and N-cadherin; and upregulation of protein signal intensity by western blotting. By using microfabrication and microfluidics to control cell shape and alignment on an elastic substrate, we found greater effects for transverse than for longitudinal stretch in regulating sarcomere organization, hypertrophy, and cell-to-cell junctions.
Flap endonucleases pass 5′-flaps through a flexible arch using a disorder-thread-order mechanism to confer specificity for free 5′-ends

Flap endonucleases (FENs), essential for DNA replication and repair, recognize and remove RNA or DNA 5′-flaps. Related to FEN specificity for substrates with free 5′-ends, but controversial, is the role of the helical arch observed in varying conformations in substrate-free FEN structures. Conflicting models suggest either 5′-flaps thread through the arch, which when structured can only accommodate single-stranded (ss) DNA, or the arch acts as a clamp. Here we show that free 5′-termini are selected using a disorder-thread-order mechanism. Adding short duplexes to 5′-flaps or 3′-streptavidin does not markedly impair the FEN reaction. In contrast, reactions of 5′-streptavidin substrates are drastically slowed. However, when added to premixed FEN and 5′-biotinylated substrate, streptavidin is not inhibitory and complexes persist after challenge with unlabelled competitor substrate, regardless of flap length or the presence of a short duplex. Cross-linked flap duplexes that cannot thread through the structured arch react at modestly reduced rate, ruling out mechanisms involving resolution of secondary structure. Combined results explain how FEN avoids cutting template DNA between Okazaki fragments and link local FEN folding to catalysis and specificity: the arch is disordered when flaps are threaded to confer specificity for free 5′-ends, with subsequent ordering of the arch to catalyze hydrolysis.
A General Method of Constructing the Reversible Full-Adder

The reversible gates, attracting people’s attention increasingly, have been widely used in low-power CMOS design, optical computing and quantum computing. In many existing literatures, only the methods of constructing certain specific reversible full-adders were presented, while we proposed a general approach to construct the reversible full-adder. According to the approach, we can realize a variety of reversible full-adders flexibly with only two reversible gates and two garbage outputs, which have improvements in the gate count and garbage count and can reduce the cost of network.
Families Coping With Alcohol and Substance Abuse

Aprominent approach to the study of alcohol and families involves a biopsychosocial focus (Zucker, Boyd, & Howard, 1994). This perspective organizes the prediction of pathological alcohol involvement (and, by extension, more general substance abuse) through consideration of biological contributions, psychological factors, and social influences. Conjoining this model with the family stress and coping model (see McKenry & Price, Chapter 1, this volume) acknowledges the contribution of these biopsychosocial factors at each juncture: stressor, resource, perception, coping, and managing. Because our focus in this chapter is on families coping with alcohol and substance abuse, we review several aspects of coping: (a) the factors that give rise to the need for coping, (b) the factors that contribute to coping, and, (c) the outcomes of coping efforts. We pay particular attention to the mediating and moderating effects that intervene between two variables, such as those that occur when associations between parent drinking and offspring drinking are mediated or modified by other variables (Baron & Kenny, 1986).
Adult mouse basal forebrain harbors two distinct cholinergic populations defined by their electrophysiology

We performed whole-cell recordings from basal forebrain (BF) cholinergic neurons in transgenic mice expressing enhanced green fluorescent protein (eGFP) under the control of the choline acetyltransferase promoter. BF cholinergic neurons can be differentiated into two electrophysiologically identifiable subtypes: early and late firing neurons. Early firing neurons (∼70%) are more excitable, show prominent spike frequency adaptation and are more susceptible to depolarization blockade, a phenomenon characterized by complete silencing of the neuron following initial action potentials. Late firing neurons (∼30%), albeit being less excitable, could maintain a tonic discharge at low frequencies. In voltage clamp analysis, we have shown that early firing neurons have a higher density of low voltage activated (LVA) calcium currents. These two cholinergic cell populations might be involved in distinct functions: the early firing group being more suitable for phasic changes in cortical acetylcholine release associated with attention while the late firing neurons could support general arousal by maintaining tonic acetylcholine levels.