Miracle-dz/UniFAR-FTUs huggingface dataset:
Three Hundred and One Consecutive Extended Right Hepatectomies: Evaluation of Outcome Based on Systematic Liver Volumetry

Objective(s):This study aimed to determine the effect of preoperative liver volumetry on postoperative outcomes after extended right hepatectomy. Primary end point was to evaluate whether future liver remnant (FLR)/standardized liver volume ratio (sFLR) >20% is sufficient for a safe hepatic resection. Secondary end point was to assess whether preoperative portal vein embolization (PVE) is associated with improved outcome in patients with initial sFLR ≤20%. Background Data:An sFLR >20% of the total liver volume has been proposed as sufficient for safe hepatic resection, but this concept has not been validated in a large series. In addition, recent reports suggest preoperative PVE is indicated for sFLR <30%. Methods:The impact of sFLR and PVE on short-term outcomes (postoperative complications, liver insufficiency, and 90-day mortality) was analyzed in 301 consecutive patients after extended right hepatectomy. Liver volumetry accounted for partial resection of segment IV. Liver insufficiency was defined as peak postoperative serum bilirubin >7 mg/dL. Predictors of liver insufficiency were identified by multivariate logistic regression. Results:Postoperative liver insufficiency occurred in 45 patients (15%) and accounted for 61% of deaths. Among 290 patients who underwent liver volumetry, sFLR was <20% in 38 patients, 20.1% to 30% in 144, and ≥30% in 108. Rates of postoperative liver insufficiency and death from liver failure were similar between patients with sFLR 20.1% to 30% and sFLR ≥30% but higher in patients with sFLR ≤20% (P < 0.05). Postoperative outcomes were similar between patients with increase in sFLR from ≤20% to >20% after PVE and patients with initial sFLR >20%. Multivariate analysis revealed that body mass index >25 kg/m2, intraoperative blood transfusion, and sFLR ≤20% (odds ratio = 3.18; 95% CI, 1.34–7.54) independently predicted postoperative liver insufficiency. Conclusions:Systematic measurement of FLR volume is important to select patients for PVE and extended right hepatectomy. A sFLR >20% is sufficient for safe hepatic resection and sFLR 20.1% to 30% is not an indication for preoperative PVE.
Niche-tracking migrants and niche-switching residents: evolution of climatic niches in New World warblers (Parulidae)

Differences in life-history traits between tropical and temperate lineages are often attributed to differences in their climatic niche dynamics. For example, the more frequent appearance of migratory behaviour in temperate-breeding species than in species originally breeding in the tropics is believed to have resulted partly from tropical climatic stability and niche conservatism constraining tropical species from shifting their ranges. However, little is known about the patterns and processes underlying climatic niche evolution in migrant and resident animals. We evaluated the evolution of overlap in climatic niches between seasons and its relationship to migratory behaviour in the Parulidae, a family of New World passerine birds. We used ordination methods to measure seasonal niche overlap and niche breadth of 54 resident and 49 migrant species and used phylogenetic comparative methods to assess patterns of climatic niche evolution. We found that despite travelling thousands of kilometres, migrants tracked climatic conditions across the year to a greater extent than tropical residents. Migrant species had wider niches than resident species, although residents as a group occupied a wider climatic space and niches of migrants and residents overlapped extensively. Neither breeding latitude nor migratory distance explained variation among species in climatic niche overlap between seasons. Our findings support the notion that tropical species have narrower niches than temperate-breeders, but does not necessarily constrain their ability to shift or expand their geographical ranges and become migratory. Overall, the tropics may have been historically less likely to experience the suite of components that generate strong selection pressures for the evolution of migratory behaviour.
Use of tn5 mutants to assess the role of the dissimilatory nitrite reductase in the competitive abilities of two pseudomonas strains in soil

We examined the influence of soil aeration state and plant root presence on the comparative survival of wild-type bacteria and isogenic Tn5 (Nir(sup-)) mutants lacking the ability to synthesize nitrite reductase. Two denitrifying Pseudomonas strains with different nitrite reductase types were used. Enumeration of bacteria in sterile and nonsterile soils was based on differential antibiotic resistance. The validity of the bacterial models studied (i.e., equal growth of wild-type and mutant bacteria under aerobic conditions and significantly better growth of wild-type bacteria under denitrifying conditions) was verified in pure-culture studies. In sterile soil, both strains survived better under aerobic than under anaerobic conditions. The lower efficiency of denitrification than O(inf2) respiration in supporting bacterial growth explained this result, and the physical heterogeneity of soil did not strongly modify the results obtained in pure-culture studies. In nonsterile soil, one of the Pseudomonas strains survived better under anaerobic conditions while the other competed equally with the indigenous soil microflora under aerobic and anaerobic conditions. However, when the Nir(sup-)-to-total inoculant ratios (wild type plus Nir(sup-) mutant) were analyzed, it appeared that the presence of nitrite reductase conferred on both Pseudomonas strains a competitive advantage for anaerobic environment or rhizosphere colonization. This is the first attempt to demonstrate with isogenic nondenitrifying mutants that denitrification can contribute to the persistence and distribution of bacteria in fluctuating soil environments.
Heroin crystal nephropathy

In this paper we present an interesting case of acute kidney injury and severe metabolic alkalosis in a patient with a history of heavy heroin abuse. Urine microscopy showed numerous broomstick-like crystals. These crystals are also identified in light and electron microscopy. We hypothesize that heroin crystalizes in an alkaline pH, resulting in tubular obstruction and acute kidney injury. Management is mainly supportive as there is no known specific therapy for this condition. This paper highlights the utility of urine microscopy in diagnosing the etiology of acute kidney injury and proposes a novel disease called heroin crystal nephropathy.
An Empirical Study on the Correctness of Formally Verified Distributed Systems

Recent advances in formal verification techniques enabled the implementation of distributed systems with machine-checked proofs. While results are encouraging, the importance of distributed systems warrants a large scale evaluation of the results and verification practices. This paper thoroughly analyzes three state-of-the-art, formally verified implementations of distributed systems: Iron-Fleet, Verdi, and Chapar. Through code review and testing, we found a total of 16 bugs, many of which produce serious consequences, including crashing servers, returning incorrect results to clients, and invalidating verification guarantees. These bugs were caused by violations of a wide-range of assumptions on which the verified components relied. Our results revealed that these assumptions referred to a small fraction of the trusted computing base, mostly at the interface of verified and unverified components. Based on our observations, we have built a testing toolkit called PK, which focuses on testing these parts and is able to automate the detection of 13 (out of 16) bugs.
Spray Characterization of Palm Olein/Diesel Blends under Various Injection Pressures

The Sauter Mean Diameter (SMD) and spray cone angle are two important parameters that characterize spray performance. The objective of this study is to characterize palm olein/diesel blends spray in terms of spray angle and SMD under different injection pressures using a hollow cone pressure swirl atomizer. The physical properties of five diesel/palm olein blends, namely B5, B10, B15, B20 and B25 were measured and their spray characteristics were tested at injection pressures of 0.8MPa, 1.0MPa, and 1.2MPa under ambient atmospheric condition. The results were compared to spray established using petroleum diesel fuel. The SMD was measured using a phase Doppler analyzer (PDA). The spray cone angle was visualized using a digital single-lens reflex (DSLR) camera. The results indicated that petroleum diesel fuel had the widest cone angle followed by B5, B10, B15, B20 and B25 under the same injection pressure. Additionally, when the injection pressure increases from 0.8MPa to 1.2MPa, the spray cone angle widen accordingly. It is concluded that high content of palm olein in the palm biofuel blends increases viscosity and surface tension and hence higher value of SMD and narrower spray cone angle was generated. An increase in injection pressure resulted in smaller droplet SMD and wider spray cone angle.
Can Abundance of Protists Be Inferred from Sequence Data: A Case Study of Foraminifera

Protists are key players in microbial communities, yet our understanding of their role in ecosystem functioning is seriously impeded by difficulties in identification of protistan species and their quantification. Current microscopy-based methods used for determining the abundance of protists are tedious and often show a low taxonomic resolution. Recent development of next-generation sequencing technologies offered a very powerful tool for studying the richness of protistan communities. Still, the relationship between abundance of species and number of sequences remains subjected to various technical and biological biases. Here, we test the impact of some of these biological biases on sequence abundance of SSU rRNA gene in foraminifera. First, we quantified the rDNA copy number and rRNA expression level of three species of foraminifera by qPCR. Then, we prepared five mock communities with these species, two in equal proportions and three with one species ten times more abundant. The libraries of rDNA and cDNA of the mock communities were constructed, Sanger sequenced and the sequence abundance was calculated. The initial species proportions were compared to the raw sequence proportions as well as to the sequence abundance normalized by rDNA copy number and rRNA expression level per species. Our results showed that without normalization, all sequence data differed significantly from the initial proportions. After normalization, the congruence between the number of sequences and number of specimens was much better. We conclude that without normalization, species abundance determination based on sequence data was not possible because of the effect of biological biases. Nevertheless, by taking into account the variation of rDNA copy number and rRNA expression level we were able to infer species abundance, suggesting that our approach can be successful in controlled conditions.
Prevalence of genital human papillomavirus among rural and urban populations in southern Yunnan province, China

This study was designed to investigate and compare the HPV prevalence, genotypes distribution and associated risk factors in rural and urban women living in Xishuang Banna district, in the province of Yunnan. A total of 177 and 190 women from rural and urban areas were engaged, respectively. HPV DNA was amplified using the L1 consensus primers system (MY09/11 and GP5/6) and HPV GenoArray test was conducted for genotyping. Proportions were compared by chi-square test, and logistic regression was used to evaluate risk factors. A total of 54 women were positive for HPV DNA. Among rural women, 23 women were positive for HPV infection, of which 21 showed a single infection and 2 had a multiple infection. HPV-16 (10/23) was the most prevalent genotype followed by HPV-52 (5/23), and HPV-58 (5/23). Urban women had a higher infection rate for overall HPV (31/54) and for multiple genotype infection (8/31). HPV-52 (9/31) was the most prevalent genotype followed by HPV-39 (7/31) and HPV-68 (5/31). The age-specific HPV prevalence was also different between rural and urban women. In urban area, women with age <35 years had the highest HPV prevalence, which declined thereafter as age advanced. However, in rural women the highest HPV prevalence was observed in an older age group (>56 years). Ethnicity, smoking and parity were significantly associated with HPV infection among urban women. Our study demonstrates that HPV prevalence and genotype distribution varies among women from rural and urban areas in the south of Yunnan.
Ligand-dependent occupancy of the retinoic acid receptor beta 2 promoter in vivo

Retinoic acid (RA) activates transcription of the RA receptor beta 2 (RAR beta 2) gene in embryonal carcinoma (EC) cells. This activation involves binding of the RAR/retinoid X receptor (RAR/RXR) heterodimer to the RA-responsive element (beta RARE). Dimethyl sulfate-based genomic footprinting was performed to examine occupancy of this promoter in P19 EC cells. No footprint was detected at the beta RARE prior to RA treatment, but a footprint was detected within the first hour of RA treatment. Concomitantly, other elements in the promoter, the cyclic AMP-responsive element and tetradecanoyl phorbol acetate-like-responsive element became footprinted. Footprints at these elements were induced by RA without requiring new protein synthesis and remained for the entire duration of RA treatment but rapidly reversed upon withdrawal of RA. A delayed protection observed at the initiator site was also reversed upon RA withdrawal. The RA-inducible footprint was not due to induction of factors that bind to these element, since in vitro assays showed that these factors are present in P19 cell extracts before RA treatment. Significantly, no RA-induced footprint was observed at any of these elements in P19 cells expressing a dominant negative RXR beta, in which RXR heterodimers are unable to bind to the beta RARE. Results indicate that binding of a liganded heterodimer receptor to the beta RARE is the initial event that allows other elements to gain access to the factors. In accordance, reporter analyses showed that a mutation in the beta RARE, but not those in other elements, abrogates RA activation of the promoter. It is likely that the RAR beta 2 promoter opens in a hierarchically ordered manner, signalled by the occupancy of liganded heterodimers.
Institutional Equity Flows, Liquidity Risk and Asset Pricing

Active participation from institutional investors is associated with an increase in market liquidity. Institutions, due to their high propensity to trade and their noninformational trading motives, improve market liquidity. On the other hand, institutions are attracted to the equity market when liquidity in this market is high. Based on such a positive correlation, this paper adopts aggregate institutional equity acquisitions as a proxy for market liquidity. Using this measure, stocks whose returns are more sensitive to shocks in institutional equity acquisition are found to offer higher returns, to compensate for exposure to liquidity risk. The positive spread between portfolios with high and low market liquidity risk exposures, termed IML (Illiquid Minus Liquid), persists after risk adjustment. Furthermore, the Fama-MacBeth 2-stage test yields an annual premium above 4% for IML, in the presence of the Fama-French factors and the momentum factor. IML is also shown to help explain momentum profits. Therefore, this study complements the existing literature that addresses the relationship between individual stock returns and the stocks’ own (il-)liquidity attributes, and confirms that not only liquidity characteristics, but also systematic liquidity risk, affects the pricing of securities.
The Immunogenicity of a Single Dose of Hepatitis A Virus Vaccines (Havrix® and Epaxal®) in Korean Young Adults

Purpose Assessing the immunogenicity of a single dose of hepatitis A virus (HAV) vaccines is important because some people receive only a single dose. However, previous studies have shown variable results and have not examined the effects of demographic characteristics other than gender. This study was performed to examine the immunogenicity of a single dose of HAV vaccine according to the vaccine type and demographic characteristics in young adults. Materials and Methods Seronegative medical school students were randomly allocated to receive either Havrix or Epaxal. Results After approximately 11 months, the seroconversion rate in 451 participants was 80.7%. In men, the Havrix group showed a significantly higher seroconversion rate (81.9%) than the Epaxal group (69.2%), whereas both vaccine groups showed similarly high immunogenicity in women (Havrix: 90.1%, Epaxal: 92.9%; P for interaction=0.062). According to the results of a multivariate analysis, Epaxal showed significantly lower immunogenicity than Havrix only in men. Age, obesity, drinking, smoking, and follow-up time did not significantly affect seroconversion in either gender. Conclusion The seroconversion rate of single-dose HAV vaccines was low in men, particularly in those who received Epaxal. Our results suggest that gender effects should be considered when comparing the immunogenicity of different HAV vaccines.
Global S-wave tomography using receiver pairs: an alternative to get rid of earthquake mislocation

Global seismic tomography suffers from uncertainties in earthquake parameters routinely published in seismic catalogues. In particular, errors in earthquake location and origin-time may lead to strong biases in measured body wave delay-times and significantly pollute tomographic models. Common ways of dealing with this issue are to incorporate source parameters as additional unknowns into the linear tomographic equations, or to seek combinations of data to minimize the influence of source mislocations. We propose an alternative, physically-based method to desensitize direct S-wave delay-times to errors in earthquake location and origin-time. Our approach takes advantage of the fact that mislocation delay-time biases depend to first order on the earthquake-receiver azimuth, and to second order on the epicentral distance. Therefore, for every earthquake, we compute S-wave differential delay-times between optimized receiver pairs, such that a large part of their mislocation delay-time biases cancels out (for example origin-time fully subtracts out), while the difference of their sensitivity kernels remains sensitive to the model parameters of interest. Considering realistic, randomly distributed source mislocation vectors, as well as various levels of data noise and different synthetic Earths, we demonstrate that mislocation-related model errors are highly reduced when inverting for such differential delay-times, compared to absolute ones. The reduction is particularly rewarding for imaging the upper-mantle and transition zone. We conclude that using optimized receiver pairs is a suitable, low cost alternative to get rid of errors on earthquake location and origin-time for teleseismic direct S-wave traveltimes. Moreover, it can partly remove unilateral rupture propagation effects in cross-correlation delay-times, since they are similar to mislocation effects.
Improvements in the quality of co-ordination of nursing care following implementation of the Resident Assessment Instrument in Dutch nursing homes.

AIM To study the effect of implementation of the Resident Assessment Instrument (RAI) on the quality of co-ordination of nursing care in Dutch nursing homes. BACKGROUND The Resident Assessment Instrument (RAI) was designed to improve the quality of care and quality of life in nursing homes. Until now, only noncontrolled studies on the effects of implementation of the RAI have been carried out. DESIGN/METHODS Quasi-experimental; intervention wards with RAI compared with wards with no intervention. We used the co-ordination of nursing care instrument, which includes measures for case history, care plan, end of shift report, communication, patient allocation and patient report. The scores on these scales represent the quality of nursing procedures on a ward. The measurements were done 1 month before and 8 months after RAI-implementation in 18 wards in 10 nursing homes in the Netherlands. RESULTS Out of 348 somatic patients on the participating wards who met the inclusion criteria and signed an informed consent, 278 could be measured at the first and 218 at the second data collection. 175 residents could participate twice. We used a meta-analysis technique to study the mean differences between eight couples of RAI/control wards before and after the intervention. The mean difference scores showed significant positive improvement in the RAI group for case history, there were minor (not statistically significant) improvements for all other scores and the total score. These results are encouraging especially in light of the fact that RAI-implementation in all the experimental wards did not proceed according to plan, owing to staffing and software problems. CONCLUSIONS We conclude that the RAI has the potential to improve the quality of co-ordination of care in nursing homes.
Templateless Non-Rigid Reconstruction and Motion Tracking With a Single RGB-D Camera

We present a novel templateless approach for nonrigid reconstruction and motion tracking using a single RGB-D camera. Without any template prior, our system achieves accurate reconstruction and tracking for considerably deformable objects. To robustly register the input sequence of partial depth scans with dynamic motion, we propose an efficient local-to-global hierarchical optimization framework inspired by the idea of traditional structure-from-motion. Our proposed framework mainly consists of two stages, local nonrigid bundle adjustment and global optimization. To eliminate error accumulation during the nonrigid registration of loop motion sequences, we split the full sequence into several segments and apply local nonrigid bundle adjustment to align each segment locally. Global optimization is then adopted to combine all segments and handle the drift problem through loop-closure constraint. By fitting to the input partial data, a deforming 3D model sequence of dynamic objects is finally generated. Experiments on both synthetic and real test data sets and comparisons with state of the art demonstrate that our approach can handle considerable motions robustly and efficiently, and reconstruct high-quality 3D model sequences without drift.
Ca2+ current enhancement by alpha 2/delta and beta subunits in Xenopus oocytes: contribution of changes in channel gating and alpha 1 protein level.

1. A combined biochemical and electrophysiological approach was used to determine the mechanism by which the auxiliary subunits of Ca2+ channel enhance the macroscopic Ca2+ currents. Xenopus oocytes were injected with RNA of the main pore‐forming subunit (cardiac: alpha 1C), and various combinations of RNAs of the auxiliary subunits (alpha 2/delta and beta 2A). 2. The single channel open probability (Po; measured at 0 mV) was increased approximately 3‐, approximately 8‐ and approximately 35‐fold by alpha 2/delta, beta 2A and alpha 2/delta+beta 2A, respectively. The whole‐cell Ca2+ channel current was increased approximately 8‐ to 10‐fold by either alpha 2/delta or beta 2A, and synergistically > 100‐fold by alpha 2/delta+beta 2A. The amount of 35S‐labelled alpha 1 protein in the plasma membrane was not changed by coexpression of beta 2A, but was tripled by coexpression of alpha 2/delta (either with or without beta). 3. We conclude that the increase in macroscopic current by alpha 2/delta is equally due to changes in amount of alpha 1 in the plasma membrane and an increase in Po, whereas all of the effect of beta 2A is due to an increase in Po. The synergy between alpha 2/delta and beta in increasing the macroscopic current is due mainly to synergistic changes in channel gating.