Miracle-dz/UniFAR-FTUs huggingface dataset:
Live Attenuated Leishmania donovani Centrin Knock Out Parasites Generate Non-inferior Protective Immune Response in Aged Mice against Visceral Leishmaniasis

Background Visceral leishmaniasis (VL) caused by the protozoan parasite Leishmania donovani causes severe disease. Age appears to be critical in determining the clinical outcome of VL and at present there is no effective vaccine available against VL for any age group. Previously, we showed that genetically modified live attenuated L. donovani parasites (LdCen-/-) induced a strong protective innate and adaptive immune response in young mice. In this study we analyzed LdCen-/- parasite mediated modulation of innate and adaptive immune response in aged mice (18 months) and compared to young (2 months) mice. Methodology Analysis of innate immune response in bone marrow derived dendritic cells (BMDCs) from both young and aged mice upon infection with LdCen-/- parasites, showed significant enhancement of innate effector responses, which consequently augmented CD4+ Th1 cell effector function compared to LdWT infected BMDCs in vitro. Similarly, parasitized splenic dendritic cells from LdCen-/- infected young and aged mice also revealed induction of proinflammatory cytokines (IL-12, IL-6, IFN-γ and TNF) and subsequent down regulation of anti-inflammatory cytokine (IL-10) genes compared to LdWT infected mice. We also evaluated in vivo protection of the LdCen-/- immunized young and aged mice against virulent L. donovani challenge. Immunization with LdCen-/- induced higher IgG2a antibodies, lymphoproliferative response, pro- and anti-inflammatory cytokine responses and stimulated splenocytes for heightened leishmanicidal activity associated with nitric oxide production in young and aged mice. Furthermore, upon virulent L. donovani challenge, LdCen-/- immunized mice from both age groups displayed multifunctional Th1-type CD4 and cytotoxic CD8 T cells correlating to a significantly reduced parasite burden in the spleen and liver compared to naïve mice. It is interesting to note that even though there was no difference in the LdCen-/- induced innate response in dendritic cells between aged and young mice; the adaptive response specifically in terms of T cell and B cell activation in aged animals was reduced compared to young mice which correlated with less protection in old mice compared to young mice. Conclusions Taken together, LdCen-/- immunization induced a significant but diminished host protective response in aged mice after challenge with virulent L. donovani parasites compared to young mice.
Deregulation, Misallocation, and Size: Evidence from India

This paper examines the impact of the deregulation of compulsory industrial licensing in India on firm-size dynamics and reallocation of resources within industries. Following deregulation, resource misallocation declines and the left-hand tail of the firm-size distribution thickens significantly, which suggests increased entry by small firms. However, the dominance and growth of large incumbent firms remains unchallenged. Quantile regressions reveal that the distributional effects of deregulation on firm size are significantly nonlinear. The reallocation of market shares toward a small number of large firms and a large number of small firms is characterized as the shrinking middle in Indian manufacturing. Small and medium-sized firms may continue to face constraints in their attempts to grow.
The use of sirolimus in the treatment of giant cystic lymphangioma

Rationale: Lymphatic malformations (LMs) are rare and benign anomalies resulting from the defective embryological development of the primordial lymphatic structures. Due to their permeative growth throughout all tissue layers, treatment is often challenging. Small asymptomatic lesions can be conservatively managed, while symptomatic lesions require active management. Surgery has been historically considered the treatment of choice, but today less invasive therapeutic options are preferred (sclerotherapy, laser therapy, oral medications). However, there are not uniform therapeutic protocols. Sirolimus is an oral medication that has been reported to be effective in the recent literature. Here we present the case of 4 newborns with giant multicystic lymphangioma treated with oral sirolimus after surgical resection had failed. Patient concerns: At birth the LMs were clinically appreciated as giant masses involving different organs and structures. Diagnoses: All patients had a prenatal diagnosis of giant multicystic lymphangioma confirmed at histological and cytological analysis. Interventions: Patients were treated with oral sirolimus after unsuccessful surgical resection. Outcomes: In all patients, sirolimus determined an overall reduction of the mass and a global involution from the macro- to the microcystic composition. Sirolimus was safe and poor disadvantages had been observed. The main and isolated adverse effect at laboratory analysis was progressive dyslipidemia, with increasing levels of total cholesterol and triglycerides. Lessons: To date, our experience with sirolimus in the management of LMs is favorable. We recommend the use of sirolimus after unsuccessful surgical excision have been tried or when the surgical approach is not feasible. A multidisciplinary follow-up is needed to monitor disease evolution.
Quantification of Massive Seasonal Aggregations of Blacktip Sharks (Carcharhinus limbatus) in Southeast Florida

Southeast Florida witnesses an enormous seasonal influx of upper trophic level marine predators each year as massive aggregations of migrating blacktip sharks (Carcharhinus limbatus) overwinter in nearshore waters. The narrow shelf and close proximity of the Gulf Stream current to the Palm Beach County shoreline drive tens of thousands of sharks to the shallow, coastal environment. This natural bottleneck provides a unique opportunity to estimate relative abundance. Over a four year period from 2011–2014, an aerial survey was flown approximately biweekly along the length of Palm Beach County. A high definition video camera and digital still camera mounted out of the airplane window provided a continuous record of the belt transect which extended 200 m seaward from the shoreline between Boca Raton Inlet and Jupiter Inlet. The number of sharks within the survey transect was directly counted from the video. Shark abundance peaked in the winter (January-March) with a maximum in 2011 of 12,128 individuals counted within the 75.6 km-2 belt transect. This resulted in a maximum density of 803.2 sharks km-2. By the late spring (April-May), shark abundance had sharply declined to 1.1% of its peak, where it remained until spiking again in January of the following year. Shark abundance was inversely correlated with water temperature and large numbers of sharks were found only when water temperatures were less than 25°C. Shark abundance was also correlated with day of the year but not with barometric pressure. Although shark abundance was not correlated with photoperiod, the departure of the sharks from southeast Florida occurred around the vernal equinox. The shark migration along the United States eastern seaboard corresponds spatially and temporally with the spawning aggregations of various baitfish species. These baseline abundance data can be compared to future studies to determine if shark population size is changing and if sharks are restricting their southward migration as global water temperatures increase.
The Voice Conversion Challenge 2016

This paper describes the Voice Conversion Challenge 2016 devised by the authors to better understand different voice conversion (VC) techniques by comparing their performance on a common dataset. The task of the challenge was speaker conversion, i.e., to transform the voice identity of a source speaker into that of a target speaker while preserving the linguistic content. Using a common dataset consisting of 162 utterances for training and 54 utterances for evaluation from each of 5 source and 5 target speakers, 17 groups working in VC around the world developed their own VC systems for every combination of the source and target speakers, i.e., 25 systems in total, and generated voice samples converted by the developed systems. These samples were evaluated in terms of target speaker similarity and naturalness by 200 listeners in a controlled environment. This paper summarizes the design of the challenge, its result, and a future plan to share views about unsolved problems and challenges faced by the current VC techniques.
Color stability of ceramic brackets immersed in potentially staining solutions

OBJECTIVE: To assess the color stability of five types of ceramic brackets after immersion in potentially staining solutions. METHODS: Ninety brackets were divided into 5 groups (n = 18) according to brackets commercial brands and the solutions in which they were immersed (coffee, red wine, coke and artificial saliva). The brackets assessed were Transcend (3M/Unitek, Monrovia, CA, USA), Radiance (American Orthodontics, Sheboygan, WI, USA), Mystique (GAC International Inc., Bohemia, NY, USA) and Luxi II (Rocky Mountain Orthodontics, Denver, CO, USA). Chromatic changes were analyzed with the aid of a reflectance spectrophotometer and by visual inspection at five specific time intervals. Assessment periods were as received from the manufacturer (T0), 24 hours (T1), 72 hours (T2), as well as 7 days (T3) and 14 days (T4) of immersion in the aforementioned solutions. Results were submitted to statistical analysis with ANOVA and Bonferroni correction, as well as to a multivariate profile analysis for independent and paired samples with significance level set at 5%. RESULTS: The duration of the immersion period influenced color alteration of all tested brackets, even though these changes could not always be visually observed. Different behaviors were observed for each immersion solution; however, brackets immersed in one solution progressed similarly despite minor variations. CONCLUSIONS: Staining became more intense over time and all brackets underwent color alterations when immersed in the aforementioned solutions.
Solid-Pseudopapillary Tumor of the Pancreas: Immunohistochemical Localization of Neuroendocrine Markers and CD10

To clarify the neuroendocrine differentiation and CD10 expression in solid-pseudopapillary tumors (SPTs) of the pancreas, we performed immunohistochemical analysis in 19 such tumors, including one solid-pseudopapillary carcinoma (SPC), along with 20 pancreatic neuroendocrine tumors (PNTs), six acinar cell carcinomas (ACCs), and one pancreatoblastoma (PB). We used antisera directed against CD56, synaptophysin, protein gene product 9.5, the alpha-subunit of Go protein, chromogranin A, CD10, trypsin, chymotrypsin, various cytokeratins (CKs), CA19-9, vimentin, and alpha-1-antitrypsin (AAT). All SPTs exhibited immunoreactivity for CD56 and CD10, and 15 expressed other neuroendocrine markers focally with the exception of chromogranin A. Frequent clustering of synaptophysin-positive cells was noted. Two cases contained a peculiar nodule that cytomorphologically and immunohistochemically resembled PNT. CD10-positive cells were scarce in one SPC. PNTs were CD56-positive, but often with faint intensity, and staining for other neuroendocrine markers, including chromogranin A, was diffusely positive. CD10 was detected, mostly in a focal pattern, in five PNTs. Pan-CK, CK8, CK18, and CK19 were more frequently demonstrated in PNT than SPT. Vimentin and AAT were often identified in PNT as well and were not specific for SPT. ACCs were CD56-negative, with the exception of one case designated as a mixed acinar-endocrine carcinoma. PB was focally positive for CD56 at the periphery of the tumor nests. Four ACCs and one PB exhibited focal CD10 reactivity. This study demonstrated the unique immunohistochemical features of SPT. Our results also suggest that SPT exhibits, at least focally, neuroendocrine differentiation, and that these neuroendocrine markers and CD10 are diagnostically useful.
A Stability Property for Coefficients in Kronecker Products of Complex Sn Characters

In this note we make explicit a stability property for Kronecker coefficients that is implicit in a theorem of Y. Dvir. Even in the simplest nontrivial case this property was overlooked despite of the work of several authors. As applications we give a new vanishing result and a new formula for some Kronecker coefficients.
Biasing restricted Boltzmann machines using Gaussian filters to learn invariant visual features

Advances in unsupervised learning have allowed the efficient learning of feature representations directly from large sets of unlabeled data instead of using traditional handcrafted features. However, improving algorithms to increase the quality of these representations in the absence of labeled data is still an area of active research. This paper evaluates visual features learned through unsupervised learning, specifically comparing regularization and preprocessing methods using Gaussian filters on a single-layer network. Using the restricted Boltzmann machine as the unsupervised learning mechanism, features emerging through training on natural videos, with different biasing and preprocessing based on Gaussian filters, are compared by metrics to measure invariance as well as classification performance on standard datasets. When Gaussian filters are convolved with adjacent hidden layer activations from a single example during training, topographies begin to emerge where adjacent features become tuned to slightly varying stimuli. 1D, 2D, and 3D topographies are compared. When a Gaussian low-pass filter is applied to activations from a single hidden node across frames drawn from video, features that are more invariant to transformations are produced. Finally, when Gaussian filters are applied to the visible nodes, images become blurrier; learning from these images also leads to invariant features. The networks are trained using the Hollywood2 video dataset, and tested on image classification of the static CIFAR-10 and STL-10 datasets. To prove that the improvements are independent of the dataset, the networks are shown to produce similar results when trained on the CIFAR-10 dataset. The induction of topography or simple image blurring via Gaussian filters during training produce better discriminative features as evidenced by the consistent and notable increase in classification results that they produce. Also, in the visual domain, invariant features are desirable such that objects can be classified accurately despite transformations. It is found that most of the compared methods produce more invariant features, however, classification accuracy does not correlate to invariance.
Cardiac bone marrow cell therapy: the proof of the pudding remains in the eating.

This editorial refers to ‘Intracoronary infusion of mononuclear cells from bone marrow or peripheral blood compared with standard therapy in patients after acute myocardial infarction treated by primary percutaneous coronary intervention: results of the randomized controlled HEBE trial’[†][1], by A. Hirsch et al. , on page 1736 , and ‘Intracoronary autologous mononucleated bone marrow cell infusion for acute myocardial infarction: results of the randomized multicenter BONAMI trial’[‡][2], by J. Roncalli et al. , on page 1748 During the last two decades, the substantial decline in short-term mortality in patients with acute myocardial infarction (AMI) has markedly increased the prevalence of patients who are admitted to cardiology wards with left ventricular (LV) dysfunction. Despite state-of-the-art pharmacological and device-based treatment, the injured myocardium often appears unable to reverse massive cumulative cardiac cell death and loss of contractile function. The net result is progressive ventricular dilatation and development of heart failure, which remains a major cause of morbidity and mortality.1 The inadequate endogenous regenerative capacity of the heart following extensive ischaemic injury has stimulated research efforts using stem or progenitor cells as a unique and exciting opportunity for true cardiac repair and perhaps regeneration. The field of clinical cardiac cell therapy has witnessed a remarkable and rapid progress, since the very first reports of adult stem cell transfer in small animal models almost a decade ago. The observations that intramyocardial injection of adult bone marrow cells (BMCs) can improve LV contractile function through regeneration2 or neovascularization3 have triggered great enthusiasm about a possible treatment enabling cell-based biological repair. The first generation of randomized controlled trials in patients with AMI explored the safety and efficacy of intracoronary injection of autologous mononuclear BMCs at variable time intervals following reperfusion. They almost uniformly focused on global left ventricular ejection fraction (LVEF) as a surrogate marker … [1]: #fn-2 [2]: #fn-3
Teasing Apart the Effects of Seed Size and Energy Content on Rodent Scatter-Hoarding Behavior

Scatter-hoarding rodents are known to play a crucial role in the seed dispersal of many plant species. Numerous studies have indicated that both seed size and the energy content of seeds can affect rodent foraging behavior. However, seed size is usually associated with energy content per seed, making it difficult to isolate how seed size and energy affect rodent foraging preferences. This study used 99 treatments of artificial seeds (11 seed sizes×9 levels of energy content) to tease apart the effect of seed size and energy content on rodent seed-caching behavior. Both seed traits showed significant effects, but their details depended on the stage of the rodent foraging process. Seeds with higher energy content were harvested more rapidly while seed size only had a modest effect on harvest rate. However, after harvesting, seed size showed a much stronger effect on rodent foraging behavior. Rodents’ choice of which seeds to remove and cache, as well as seed dispersal distance, seemed to reflect an optimal seed size. Our findings could be adapted in future studies to gain a better understanding of scatter-hoarding rodent foraging behavior, and the co-evolutionary dynamics between plant seed production and seed dispersers.
The red tail of carbon stars in the LMC: Models meet 2MASS and DENIS observations

Carbon stars are known to exhibit systematically redder near-infrared colours with respect to M-type stars. In the near-infrared colour-magnitude diagrams provided by the 2MASS and DENIS surveys, the LMC C-type stars draw a striking "red tail", well separated from the sequences of O-rich giants. So far, this conspicuous feature has been absent from any set of available isochrones, even the few existing ones that include the TP-AGB evolution of low- and intermediate-mass stars. To investigate such issue we simulate the complete 2MASS Ks vs. (J − Ks) data towards the LMC by means of a population synthesis approach, that relies on extended libraries of published stellar evolutionary tracks, including the TP-AGB phase. The simulations provide quite a detailed description of the several vertical "fingers" and inclined sequences seen in 2MASS data, due to both galactic foreground and LMC O-rich stars. Instead, as mentioned, the red tail of C-stars sets a major difficulty: we find that TP-AGB models with solar-scaled molecular opacities, the usual assumption of existing AGB calculations, do not succeed in reproducing this feature. Our tests indicate that the main reason for this failure should not be ascribed to empirical Teff-(J −K) transformations for C-type stars. Instead, the discrepancy is simply removed by adopting new evolutionary models that account for the changes in molecular opacities as AGB stars get enriched in carbon via the third dredge-up (Marigo 2002). In fact, simulations that adopt these models are able to reproduce, for the first time, the red tail of C-stars in near-infrared CMDs. Finally, we point out that these simulations also provide useful indications about the efficiency of the third dredge-up process, and the pulsation modes of long-period variables.
Effect of gait training with constrained-induced movement therapy (CIMT) on the balance of stroke patients

[Purpose] The purpose of the present study was to examine the effect of intensive gait training using a constrained induced movement therapy (CIMT) technique applied to the non-paretic upper extremity on the balance ability of stroke patients. [Subjects and Methods] Twenty stroke patients were randomly assigned to an experimental group or a control group. The experimental group received gait training with CIMT for 30 minutes per session, three sessions per week for four weeks, and the control group received gait training alone. [Results] The experimental group showed improvements in dynamic balance and the degree of improvement in this group was greater than that observed in the control group. Furthermore, the experimental group showed improvements in movement distances to the paretic side. On the other hand, the control group showed no significant improvements in balance indices after the intervention. [Conclusion] Gait training of stroke patients using CIMT techniques should be regarded as a treatment that can improve the balance of stroke patients.
Impact of Abcc2 (Mrp2) and Abcc3 (Mrp3) on the In vivo Elimination of Methotrexate and its Main Toxic Metabolite 7-hydroxymethotrexate

Purpose: ATP-binding cassette sub-family C member 2 [ABCC2; multidrug resistance–associated protein 2 (MRP2)] and ABCC3 (MRP3) mediate the elimination of toxic compounds, such as drugs and carcinogens, and have a large overlap in substrate specificity. We investigated the roles of Abcc2 and Abcc3 in the elimination of the anticancer drug methotrexate (MTX) and its toxic metabolite 7-hydroxymethotrexate (7OH-MTX) in vivo. Experimental Design:Abcc2;Abcc3−/− mice were generated, characterized, and used to investigate possibly overlapping or complementary roles of Abcc2 and Abcc3 in the elimination of MTX and 7OH-MTX after i.v. administration of 50 mg/kg MTX. Results:Abcc2;Abcc3−/− mice were viable and fertile. In Abcc2−/− mice, the plasma area under the curve (AUCi.v.) for MTX was 2.0-fold increased compared with wild type, leading to 1.6-fold increased urinary excretion, which was not seen in Abcc2;Abcc3−/− mice. Biliary excretion of MTX was 3.7-fold reduced in Abcc2−/− but unchanged in Abcc2;Abcc3−/− mice. The plasma AUCi.v. s of 7OH-MTX were 6.0-fold and 4.3-fold increased in Abcc2−/− and Abcc2;Abcc3−/− mice, respectively, leading to increased urinary excretion. The biliary excretion of 7OH-MTX was 5.8-fold reduced in Abcc2−/− but unchanged in Abcc2;Abcc3−/− mice. 7OH-MTX accumulated substantially in the liver of Abcc2−/− and especially Abcc2;Abcc3−/− mice. Conclusions: Abcc2 is important for (biliary) excretion of MTX and its toxic metabolite 7OH-MTX. When Abcc2 is absent, Abcc3 transports MTX and 7OH-MTX back from the liver into the circulation, leading to increased plasma levels and urinary excretion. Variation in ABCC2 and/or ABCC3 activity may therefore have profound effects on the elimination and severity of toxicity of MTX and 7OH-MTX after MTX treatment of patients.