Miracle-dz/UniFAR-FTUs huggingface dataset:
Controlling the Toll road to dendritic cell polarization

The activation of dendritic cells (DC) via Toll‐like receptors (TLRs) plays a decisive role in shaping the outcome of primary immune responses. Following TLR engagement by microbial products, DC migrate from peripheral tissues to lymphoid organs and up‐regulate major histocompatibility complex and costimulatory molecules, acquiring the unique capacity to prime pathogen‐specific, naïve T cells. In addition, DC determine the character of the ensuing immune response by secreting cytokines that drive the development of T cells into T helper cell type 1 (Th1), Th2, or T regulatory effector cells. Three major factors influence the pattern of cytokines released by DC and accordingly, the Th balance: the lineage to which DC belong; the maturation stimulus; and inflammatory mediators present at the site of infection. A major focus of this review is the capacity of DC to integrate these factors and elicit distinct classes of immune responses.
Devoted fathers or selfish lovers? Conflict between mating effort and parental care in a harem‐defending arachnid

When there is a temporal trade‐off between mating effort and parental care, theoretical models predict that intense sexual selection on males leads to reduced paternal care. Thus, high‐quality males should invest more in mating effort because they have higher chances of acquiring mates, whereas low‐quality males should bias their investment towards parental care. Once paternal care has evolved, offspring value should also influence males’ decisions to invest in offspring attendance. Here, we performed a manipulation under field conditions to investigate the factors that influence male allocation in either mating effort or parental care. We predicted that facultative paternal care in the harem‐holding harvestman Serracutisoma proximum would be negatively influenced by male attractiveness and positively influenced by offspring value. We found that attractive males were less likely to engage in egg attendance and that the higher the perceived paternity, the higher the caring frequency. Finally, egg mortality was not related to caring frequency by males, but predation pressure was much lower than that recorded in previous studies with the same population. Thus, the benefits of facultative male care may be conditional to temporal variation in the intensity of egg predation. In conclusion, males adjust their investment in either territory defence or egg attendance according to their recent mating history and perceived paternity. Our findings suggest that exclusive paternal care can evolve from facultative paternal care only if the trade‐off between mating effort and parental care is circumvented.
Anti‐Inflammatory Property of n‐Hexadecanoic Acid: Structural Evidence and Kinetic Assessment

Ester bond hydrolysis of membrane phospholipids by Phospholipase A2 and consequent release of fatty acids are the initiating steps of inflammation. It is proposed in this study that the inhibition of phospholipase A2 is one of the ways to control inflammation. Investigations are carried out to identify the mode of inhibition of phospholipase A2 by the n‐hexadecanoic acid. It may help in designing of specific inhibitors of phospholipase A2 as anti‐inflammatory agents. The enzyme kinetics study proved that n‐hexadecanoic acid inhibits phospholipase A2 in a competitive manner. It was identified from the crystal structure at 2.5 Å resolution that the position of n‐hexadecanoic acid is in the active site of the phospholipase A2. The binding constant and binding energy have also been calculated using Isothermal Titration Calorimetry. Also, the binding energy of n‐hexadecanoic acid to phospholipase A2 was calculated by in silico method and compared with known inhibitors. It may be concluded from the structural and kinetics studies that the fatty acid, n‐hexadecanoic acid, is an inhibitor of phospholipase A2, hence, an anti‐inflammatory compound. The inferences from the present study validate the rigorous use of medicated oils rich in n‐hexadecanoic acid for the treatment of rheumatic symptoms in the traditional medical system of India, Ayurveda.
Erythropoietin‐induced rheological changes of rat erythrocytes

The effects of recombinant human erythropoietin (rhEPO) on red blood cell (RBC) rheological properties were investigated in rats. Rats received intramuscular injections of 150 U/kg/d rhEPO for 5 d, following which blood samples were obtained 1, 5 or 10 d later. RBC deformability was assessed by determining cell transit times through 5‐µm micropores (CTA) and RBC shape recovery time constants via photometry, aggregation in plasma and dextran was measured by photometry and RBC electrophoretic mobility was determined in a cylindrical electrophoresis system. RBC aggregation was found to be significantly decreased on day 5 after rhEPO treatment (P < 0·05), yet was unchanged from control on days 1 and 10. Mean RBC micropore transit times remained unchanged, but the distributions of transit times were altered; compared with control, the 5th percentiles on both days 1 and 5 were decreased and the 95th percentile on day 1 was elevated. Electrophoretic mobility of RBCs in phosphate‐buffered saline was significantly increased on day 5 after rhEPO treatment (P < 0·05), with mobility measurements in dextran 500 (MW = 500 kDa) solutions suggesting that the cells' surface properties related to the formation of a ‘depletion layer’ may be altered on day 1. These results indicate that the rheological behaviour of RBC as a consequence of rhEPO treatment are temporal and are affected by the presence of reticulocytes as well as by the average age of the circulating cells.
Model-Driven Design, Refinement and Transformation of Abstract Interactions

In a model-driven design process the interaction between application parts can be described at various levels of platform-independence. At the lowest level of platform-independence, interaction is realized by interaction mechanisms provided by specific middleware platforms. At higher levels of platform-independence, interaction must be described in such a way that it can be further refined and realized onto a number of different middleware platforms, each with its particular interaction mechanisms and implementation constraints. In this paper, we investigate concepts that support interaction design at various levels of middleware-platform-independence. In addition, we propose design operations for interaction refinement. The application of these operations to source designs results in target designs that take into account implementation constraints imposed by platforms, while preserving characteristics prescribed in source designs. Target designs are related to source designs by conformance. We discuss how transformation and conformance can be related, such that transformations indeed preserve the characteristics prescribed by a source design.
Physicians' decisions to override computerized drug alerts in primary care.

BACKGROUND Although computerized physician order entry reduces medication errors among inpatients, little is known about the use of this system in primary care. METHODS We calculated the override rate among 3481 consecutive alerts generated at 5 adult primary care practices that use a common computerized physician order entry system for prescription writing. For detailed review, we selected a random sample of 67 alerts in which physicians did not prescribe an alerted medication and 122 alerts that resulted in a written prescription. We identified factors associated with the physicians' decisions to override a medication alert, and determined whether an adverse drug event (ADE) occurred. RESULTS Physicians overrode 91.2% of drug allergy and 89.4% of high-severity drug interaction alerts. In the multivariable analysis using the medical chart review sample (n = 189), physicians were less likely to prescribe an alerted medication if the prescriber was a house officer (odds ratio [OR], 0.26; 95% confidence interval [CI], 0.08-0.84) and if the patient had many drug allergies (OR, 0.70; 95% CI, 0.53-0.93). They were more likely to override alerts for renewals compared with new prescriptions (OR, 17.74; 95% CI, 5.60-56.18). We found no ADEs in cases where physicians observed the alert and 3 ADEs among patients with alert overrides, a nonsignificant difference (P =.55). Physician reviewers judged that 36.5% of the alerts were inappropriate. CONCLUSIONS Few physicians changed their prescription in response to a drug allergy or interaction alert, and there were few ADEs, suggesting that the threshold for alerting was set too low. Computerized physician order entry systems should suppress alerts for renewals of medication combinations that patients currently tolerate.
Purification and characterization of NADP-specific alcohol dehydrogenase and glutamate dehydrogenase from the hyperthermophilic archaeon Thermococcus litoralis

Thermococcus litoralis is a strictly anaerobic archaeon that grows at temperatures up to 98 degrees C by fermenting peptides. Little is known about the primary metabolic pathways of this organism and, in particular, the role of enzymes that are dependent on thermolabile nicotinamide nucleotides. In this paper we show that the cytoplasmic fraction of cell extracts contained NADP-specific glutamate dehydrogenase (GDH) and NADP-specific alcohol dehydrogenase (ADH) activities, neither of which utilized NAD as a cofactor. The GDH is composed of identical subunits having an M(r) of 45,000 and had an optimal pH and optimal temperature for glutamate oxidation of 8.0 and > 95 degrees C, respectively. Potassium phosphate (60 mM), KCl (300 mM), and NaCl (300 mM) each stimulated the rate of glutamate oxidation activity between two- and threefold. For glutamate oxidation the apparent Km values at 80 degrees C for glutamate and NADP were 0.22 and 0.029 mM, respectively, and for 2-ketoglutarate reduction the apparent Km values for 2-ketoglutarate, NADPH, and NH4+ were 0.16, 0.14, and 0.63 mM, respectively. This enzyme is the first NADP-specific GDH purified form a hyperthermophilic organism. T. litoralis ADH is a tetrameric protein composed of identical subunits having an M(r) of 48,000; the optimal pH and optimal temperature for ethanol oxidation were 8.8 and 80 degrees C, respectively. In contrast to GDH activity, potassium phosphate (60 mM), KCl (0.1 M), and NaCl (0.3 M) inhibited ADH activity, whereas (NH4)2SO4 (0.1 M) had a slight stimulating effect. This enzyme exhibited broad substrate specificity for primary alcohols, but secondary alcohols were not oxidized. (ABSTRACT TRUNCATED AT 250 WORDS)
A wavelet packet transform-based approach for interference measurement in spread spectrum wireless communication systems

This paper mainly concerns interference measurement in spread spectrum, wireless communication systems. A new digital signal-processing method is proposed, which proves to be nonintrusive and independent of the specific system considered. Thanks to the properties of the wavelet packet transform, the method is capable of extracting the interference that occurred from the spread spectrum signal, thus ensuring accurate interference magnitude and frequency estimates also in critical conditions: interference level much smaller than that characterizing the spread spectrum signal, and interference spectral content very close to the carrier frequency of the considered system.
On the predictability of coupled automata: an allegory about chaos

The authors show a sharp dichotomy between systems of identical automata with symmetric global control whose behavior is easy to predict and those whose behavior is hard to predict. The division pertains to whether the global control rule is invariant with respect to permutations of the states of the automaton. It is also shown that testing whether the global control rule has this invariance property is an undecidable problem. It is argued that there is a natural analog between complexity in the present model and chaos in dynamical systems. <<ETX>>
Developmental Profile, Morphology, and Synaptic Connectivity of Cajal–Retzius Cells in the Postnatal Mouse Hippocampus

Cajal–Retzius (CR) cells are early generated neurons, involved in the assembly of developing neocortical and hippocampal circuits. However, their roles in networks of the postnatal brain remain poorly understood. In order to get insights into these latter functions, we have studied their morphological and synaptic properties in the postnatal hippocampus of the CXCR4-EGFP mouse, where CR cells are easily identifiable. Our data indicate that CR cells are nonuniformly distributed along different subfields of the hippocampal formation, and that their postnatal decline is regulated in a region-specific manner. In fact, CR cells persist in distinct areas of fully mature animals. Subclasses of CR cells project and target either local (molecular layers) or distant regions [subicular complex and entorhinal cortex (EC)] of the hippocampal formation, but have similar firing patterns. Lastly, CR cells are biased toward targeting dendritic shafts compared with spines, and produce large-amplitude glutamatergic unitary postsynaptic potentials on γ-aminobutyric acid (GABA) containing interneurons. Taken together, our results suggest that CR cells are involved in a novel excitatory loop of the postnatal hippocampal formation, which potentially contributes to shaping the flow of information between the hippocampus, parahippocampal regions and entorhinal cortex, and to the low seizure threshold of these brain areas.
Prevalence and Correlates of Prehypertension and Hypertension among Adults in Northeastern China: A Cross-Sectional Study

Background: Prehypertension is a category between normotension and hypertension that is becoming increasingly common in China. However, limited data are available on the prevalence and correlates of prehypertension in northeastern China. Methods: A cross-sectional study using stratified, clustered multistage, and random sampling methods was performed on 17,584 participants. Results: The prevalence of prehypertension and hypertension was 36.0% and 30.8% in northeastern China, respectively. As age increased, the prevalence of prehypertension in males declined (p-trend < 0.001), in parallel to an increase in the prevalence of hypertension (p-trend < 0.001). The prevalence of hypertension for females increased as age increased (p-trend < 0.001). Logistic regression analysis showed that age, gender, location, drinking, Body Mass Index (BMI), abdominal obesity, hypertriglyceridemia, and hypercholesterolemia correlated with prehypertension and hypertension (p-trend < 0.05). Conclusions: This study revealed a high prevalence of prehypertension and hypertension in an adult population of northeastern China and some correlates of prehypertension and hypertension.
Cell block technique as a complementary method in the clinical diagnosis of cyst-like lesions of the jaw

Objective To evaluate the applicability of the cell block technique as a complementary method for presumptive diagnosis in the analysis of cyst-like aspirates from jaw lesions prior to histopathological diagnosis. Material and Methods The sample was made up of 17 cyst-like jaw lesions. After puncture, the aspirates were centrifuged, fixed in formalin, embedded in paraffin and processed. All lesions were biopsied and submitted to histopathological examination. Results In 9 cases, the cytopathological analysis using the cell block method showed a predominant presence of erythrocytes, inflammatory cells and few epithelial cells. In the other 8 cases, the cell block technique demonstrated the presence of parakeratin, whose histopathological analysis confirmed the occurrence of keratocystic odontogenic tumors (KOTs). Conclusions According to the studied cases, the cell block method was proven to be a simple, fast and low-cost technique to effectively differentiate KOTs from other lesions with similar clinical and radiographic features. The cell block technique comprises cellular preparations useful to determine a clinical diagnosis and help to develop a therapeutic plan for those lesions.
Optimizing away joins on data streams

Monitoring aggregates on network traffic streams is a compelling application of data stream management systems. Often, streaming aggregation queries involve joining multiple inputs (e.g., client requests and server responses) using temporal join conditions (e.g., within 5 seconds), followed by computation of aggregates (e.g., COUNT) over temporal windows (e.g., every 5 minutes). These types of queries help identify malfunctioning servers (missing responses), malicious clients (bursts of requests during a denial-of-service attack), or improperly configured protocols (short timeout intervals causing many retransmissions). However, while such query expression is natural, its evaluation over massive data streams is inefficient. In this paper, we develop rewriting techniques for streaming aggregation queries that join multiple inputs. Our techniques identify conditions under which expensive joins can be optimized away, while providing error bounds for the results of the rewritten queries. The basis of the optimization is a powerful but decidable theory in which constraints over data streams can be formulated. We show the efficiency and accuracy of our solutions via experimental evaluation on real-life IP network data using the Gigascope stream processing engine.
COPD prevalence and the differences between newly and previously diagnosed COPD patients in a spirometry program.

AIMS To evaluate the prevalence and severity of COPD in a primary care population participating in a spirometry program. Differences between newly and previously diagnosed COPD patients were identified. METHODS A spirometry program was conducted in 15 primary care centres. Visitors aged over 30 years who were willing to perform spirometry were included in this program. RESULTS A total of 1,526 subjects provided acceptable spirometries. COPD prevalence in our population was 18.4%, of whom 69.0% were newly diagnosed. Most patients were classified as GOLD stages I and II (26.0% and 54.0%, respectively). COPD diagnosis was related to gender (men), age (older subjects), history of repeated respiratory infection in childhood, smoking (>10 pack-years) and presence of symptoms (cough, dyspnoea, wheezing). Variables related to newly diagnosed COPD were younger age and absence of chronic cough. CONCLUSIONS A primary care spirometry program may identify a large proportion of undiagnosed COPD patients especially in the early stages of the disease. Newly diagnosed COPD patients were of younger age and presented with less symptoms. These results support the need for spirometry programs in primary care for early COPD detection.
Extreme reduction in body size and reproductive output associated with sandy substrates in two anuran species

Geographic variation in body size and reproductive traits has been reported in a wide range of organisms, including amphibians. Most studies have focused on latitudinal and/or altitudinal variation where differences in temperature and duration of the growing season are the main causes for population divergence. We describe a steep variation in body size and reproductive traits in two anuran species in southwestern Spain, associated with changes in the geological substrate. Pelobates cultripes and Bufo calamita (= Epidalea calamita ) drastically reduced their size (a 71.6% and 76.1% reduction in body mass for P. cultripes and B. calamita , respectively) in just about 60 km. This extreme size reduction was more pronounced at the boundary between two different geological substrates (hercinic and sandy soil). Mean clutch mass, egg size, and clutch size were all smaller in B. calamita populations in the sandy environment. Likewise, clutch mass and egg size were both smaller in sandy P. cultripes populations. We observed a negative correlation between size-adjusted fecundity and egg size for both species, suggesting the existence of a reproductive trade-off that could explain the differences in reproductive allocation between populations and species. In P. cultripes , small-bodied populations had relatively higher fecundities and smaller eggs than large-bodied ones, whereas in B. calamita populations from the sandy area we found both populations with high fecundity and small eggs, and populations with low fecundity and large eggs. Common environmental effects associated with the sandy substrate produce a similar reduction in size in both toad species.
The Role of Fine-Needle Aspiration Cytology in the Diagnosis of Basal Cell Carcinoma

Background/Aims. Basal cell carcinoma (BCC) is the most common malignant tumor of the skin in humans. The diagnosis of BCC is made clinically, which can then be confirmed microscopically. Biopsy or surgical excision of the lesion provides the specimen for histopathological examination, which is the mainstay for diagnosis. Fine-needle aspiration cytology (FNAC) on the other hand is an even simpler procedure, which can provide accurate diagnosis to confirm or exclude the malignancy. Methods. Here, we present our experience on the role of FNAC in diagnosing BCC. We were able to recruit 37 patients, of which 35 had BCC. Both FNAC and biopsy were obtained and then interpreted independently of one another. Results. Cytology correlated with histopathology in all cases except for 2 in which the yield was deemed inadequate. The sensitivity and specificity of fine-needle aspiration cytology for basal cell carcinoma were 94.3% and 100%, respectively. Conclusions. We, therefore, recommend this technique for the initial evaluation of a patient with suspected BCC or in cases of recurrence. The technique is cheap, quick, less invasive, and highly accurate for the diagnosis of BCC. The limitation of the technique is low yield in some of the cases.
Designs of Concrete Bridges with Multiple Box Cells Due to Torsion Using Softened Truss Model

The authors of this paper present a method for torsional design of multicell concrete bridges using a softened truss model. Earlier researchers have successfully demonstrated the development for solving single cell torsion by combining the equilibrium, compatibility, and the softened constitutive laws of concrete. By solving the simultaneous equations based on the membrane analogy, multicontinuous or separate cells can be solved.
Multi-Factor Fuzzy Time Series Model Based on Stock Volatility for Forecasting Taiwan Stock Index

Fuzzy time series have in recent years drawn many scholars' attention due to their ability can handle the time series data with incomplete, imprecise and ambiguous pattern. However, most traditional time series models employed only single variable (stock index) in forecasting, yet ignored some factors that would also affect the stock volatility. Therefore, this paper proposes a novel forecasting model using multi-factor fuzzy time series model to forecast TAIEX (Taiwan Stock Exchange Capitalization Weighted Stock index). Multi-factor fuzzy time series model is composed of three main components: stock index, trading volume and interactions between two stock markets. In order to evaluate the performance of the proposed model, the transaction records of TAIEX (Taiwan Stock Exchange Capitalization Weighted Stock index) and NASDAQ(National Association of Securities Dealers Automated Quotations) from 2000/01/04 to 2003/12/31 are used as experimental dataset and the root mean square error (RMSE) as evaluation criterion. The results show that the proposed model outperforms the listing models in accuracy for forecasting Taiwan stock market.