Miracle-dz/UniFAR-FTUs huggingface dataset:
Osteogenesis Associates With Inflammation in Early-Stage Atherosclerosis Evaluated by Molecular Imaging In Vivo

Background— Arterial calcification is associated with cardiovascular events; however, mechanisms of calcification in atherosclerosis remain obscure. Methods and Results— We tested the hypothesis that inflammation promotes osteogenesis in atherosclerotic plaques using in vivo molecular imaging in apolipoprotein E−/− mice (20 to 30 weeks old, n=35). A bisphosphonate-derivatized near-infrared fluorescent imaging agent (excitation 750 nm) visualized osteogenic activity that was otherwise undetectable by x-ray computed tomography. Flow cytometry validated the target specifically in osteoblast-like cells. A spectrally distinct near-infrared fluorescent nanoparticle (excitation 680 nm) was coinjected to simultaneously image macrophages. Fluorescence reflectance mapping demonstrated an association between osteogenic activity and macrophages in aortas of apolipoprotein E−/− mice (R2=0.93). Intravital dual-channel fluorescence microscopy was used to further monitor osteogenic changes in inflamed carotid arteries at 20 and 30 weeks of age and revealed that macrophage burden and osteogenesis concomitantly increased during plaque progression (P<0.01 and P<0.001, respectively) and decreased after statin treatment (P<0.0001 and P<0.05, respectively). Fluorescence microscopy on cryosections colocalized near-infrared fluorescent osteogenic signals with alkaline phosphatase activity, bone-regulating protein expression, and hydroxyapatite nanocrystals as detected by electron microscopy, whereas von Kossa and alizarin red stains showed no evidence of calcification. Real-time reverse-transcription polymerase chain reaction revealed that macrophage-conditioned media increased alkaline phosphatase mRNA expression in vascular smooth muscle cells. Conclusions— This serial in vivo study demonstrates the real-time association of macrophage burden with osteogenic activity in early-stage atherosclerosis and offers a cellular-resolution tool to identify preclinical microcalcifications.
Performance of the Survey Propagation-guided decimation algorithm for the random NAE-K-SAT problem

We show that the Survey Propagation-guided decimation algorithm fails to find satisfying assignments on random instances of the "Not-All-Equal-$K$-SAT" problem if the number of message passing iterations is bounded by a constant independent of the size of the instance and the clause-to-variable ratio is above $(1+o_K(1)){2^{K-1}\over K}\log^2 K$ for sufficiently large $K$. Our analysis in fact applies to a broad class of algorithms described as "sequential local algorithms". Such algorithms iteratively set variables based on some local information and then recurse on the reduced instance. Survey Propagation-guided as well as Belief Propagation-guided decimation algorithms - two widely studied message passing based algorithms, fall under this category of algorithms provided the number of message passing iterations is bounded by a constant. Another well-known algorithm falling into this category is the Unit Clause algorithm. Our work constitutes the first rigorous analysis of the performance of the SP-guided decimation algorithm. The approach underlying our paper is based on an intricate geometry of the solution space of random NAE-$K$-SAT problem. We show that above the $(1+o_K(1)){2^{K-1}\over K}\log^2 K$ threshold, the overlap structure of $m$-tuples of satisfying assignments exhibit a certain clustering behavior expressed in the form of constraints on distances between the $m$ assignments, for appropriately chosen $m$. We further show that if a sequential local algorithm succeeds in finding a satisfying assignment with probability bounded away from zero, then one can construct an $m$-tuple of solutions violating these constraints, thus leading to a contradiction. Along with (citation), this result is the first work which directly links the clustering property of random constraint satisfaction problems to the computational hardness of finding satisfying assignments.
Electrostatic spinning assembly

Spinneret for producing nanofibres from a viscous liquid using electrostatic spinning has been described in the field. Spinneret comprises a central set radially with respect to the central axis, and a narrow annular body having one or more wide are spaced axially along it. Annular body may be a disk, a ring or coil, to.
Transmission electron microscopy of fine needle aspiration biopsies of metastases. Accuracy of both techniques as established by biopsy diagnoses.

OBJECTIVE To determine the accuracy of fine needle aspiration biopsy in establishing the primary on cytology and on cytologic and electron microscopy examinations using tissue biopsy as the gold standard. STUDY DESIGN A retrospective study of 96 nonrandomly selected cases of metastases with an unknown primary was carried out. Tissue biopsy was performed subsequently for confirmation. Proper clinical correlation was part of the analysis. RESULTS The accuracy of cytology in identifying the tumor category (e.g., carcinoma, sarcoma) and tumor type (e.g., adenocarcinoma, leiomyosarcoma) was 76% and 78%, respectively, while that of electron microscopy was 95% and 91%, respectively. The primary site was correctly identified by cytology in 59% and by electron microscopy in 88% of cases, provided that clinical parameters were also considered. CONCLUSION Electron microscopy is an ancillary technique useful in increasing the accuracy of tumor classification of metastatic neoplasms identified by fine needle aspiration biopsy.
Long term results of percutaneous balloon valvoplasty of congenital aortic stenosis: independent predictors of outcome

Objective: To evaluate long term results and independent predictors of outcome of aortic valvoplasty. Design: Retrospective follow up study. Independent predictors of outcome identified by multiple logistic regression. Setting: Tertiary referral centre. Patients: 269 consecutive patients treated at the median age of 8 months (0–23 years): 80 (30%) under 4 weeks, 59 (22%) between 4 weeks and 1 year, and 130 (48%) over 1 year. The follow up period was up to 14.8 years (median 5.3, in survivors 6.4 years). Interventions: Percutaneous balloon valvoplasty with mean (SD) balloon to annulus ratio 0.97 (0.08). Main outcome measures: Restenosis ⩾ 70 mm Hg, grade 3 aortic insufficiency, cusps disruption, surgery, death, and valvoplasty failure (significant restenosis or insufficiency or surgery or death). Results: The mortality rate was 10.4% (n = 28), the restenosis rate was 16.7% (n = 45), significant insufficiency developed in 22.3% (n = 60), surgery was needed in 20.1% (n = 54), and “valvoplasty failure” occurred in 41.6% (n = 112) patients. Mean (SEM) survival probability 14.4 years after the procedure was 0.89 (0.02) and mean (SEM) probability of surgery-free survival was 0.50 (0.08). The independent predictors were as follows. For restenosis: small aortic annulus; for cusp disruption: large aortic annulus; for insufficiency: bicuspid aortic valve; for need for surgery: bicuspid aortic valve; for death: small aortic annulus, low left ventricular shortening fraction, and low sequential number of the valvoplasty; and for valvoplasty failure: small aortic annulus, bicuspid aortic valve, and high grade of mitral insufficiency. Conclusion: Independent predictors of unfavourable outcome are small aortic annulus, bicuspid aortic valve, poor function of left ventricle or mitral valve, and limited operator experience.
Visual impairment and depression among socially vulnerable older adults in Armenia

Objectives: Visual impairment in older adults is a major public health problem. Untreated visual impairment might negatively impact physical and psychological health. This study assessed the association between visual impairment and depression among socially vulnerable older adults (those aged 50 and above) in Armenia.Method: The survey and eye screenings were carried out among 339 participants who were the residents of retirement homes and single older adults in the households. The study team used Golovin–Sivtsev chart and cycloplegic skiascopy to measure visual impairment and Center for Epidemiologic Studies Depression scale to measure depression.Results: The prevalence of visual impairment in the sample was 13.3%. Almost 24.0% of participants reported depression symptoms. Participants living in the retirement homes had substantially higher rates of visual impairment (21.5%) and depression (28.0%) than those living in households (9.3% and 15.0%, respectively). The odds of having depression were higher among those with visual impairment compared to those without after adjusting for confounders (OR = 2.75; 95% CI: 1.29–5.87). Having at least one non-communicable disease was associated with depression (OR = 2.47; 95% CI: 1.28–4.75). Living in the retirement home was marginally significantly associated with having depression. Other confounders included age, gender, education, physical activity, and smoking.Conclusion: Visual impairment was significantly associated with depression in socially vulnerable older adults in Armenia. Timely eye screenings in similar population groups could lead to early detection of visual impairment and prevention of visual loss and associated mental health problems.
Post‐Agricultural Succession in El Petén, Guatemala

Abstract: We compared post‐agricultural succession across the range of farming activities practiced in Guatemala's northern lowlands: agroforestry, swidden, ranching, and input‐intensive monocultures. At 10 sites over 13 to 40 months we assessed the following characteristics of trees and shrubs that were>1 cm diameter at breast height: height, basal‐area accumulation, recruitment of all individuals and fleshy‐fruited individuals, and accumulation of all species and fleshy‐fruited species. Succession, as measured by all these response variables except height, was dramatically faster on agroforestry and swidden sites than on pastures or input‐intensive monocultures. Overall recruitment was faster for swiddens than for agroforests, but other response variables did not differ significantly between the two treatments. Regression results suggest that initial ground cover by herbs inhibited recruitment of woody colonists. The significant positive coefficient for initial basal area and the significant negative coefficient for distance from forest for accumulation of both fleshy‐fruited individuals and species are probably explained by the behavioral responses of seed‐dispersing animals. Our results suggest that the conservation strategy of discouraging swidden agriculture in favor of sedentary, input‐intensive agriculture to relieve pressure on old‐growth forest may be counterproductive over the long term.
Serologic Detection of Anti Toxoplasma gondii Infection in Diabetic Patients

Background Toxoplasmosis is caused by the Toxoplasma gondii parasite. The parasite is intracellular and can result in severe complications leading to death in immuno-deficient patients in particular. Diabetes is an important factor that increases susceptibility and risk of various infections in the host. Objectives The present study focused on the serologic detection of Toxoplasma gondii infection in diabetic patients. Materials and Methods Through a case-control study, overall 184 serum samples including 91 from diabetic cases and 93 from healthy non-diabetic controls were investigated. Cases and controls were matched for age and gender. Serum samples were tested for sugar by an enzymatic method, and IgG antibodies were tested against Toxoplasma gondii by ELISA method. Results The prevalence of IgG antibodies against Toxoplasma gondii in diabetic patients and healthy controls were 60.43% and 38% respectively. Risk of toxoplasmosis infection in diabetic patients with was two folds higher than healthy controls (RR = 2.21, 95% CI; 1.6 – 3.7, P = 0.001). Conclusions Diabetes may be caused by Toxoplasma gondii. Presence of T. gondii in the pancreas at the same time could directly undermines the pancreas cells. When β cells are destroyed, insulin secretion would then be affected. Probably the destruction of T. gondii affects nervous system and damages pancreatic cells leading to increased risk of diabetes.
Effect of Botulinum Toxin in the Treatment of Drooling: A Controlled Clinical Trial

Objective. To investigate the clinical effectiveness of botulinum neurotoxin type A (BoNT) to reduce drooling in children with cerebral palsy (CP). Methods. A controlled clinical trial was performed in which the results of single-dose BoNT injections in the submandibular glands were compared with treatment with scopolamine. Forty-five children who had CP and experienced severe drooling were enrolled. Drooling severity was measured at baseline, during application of scopolamine, and at different intervals after BoNT injections up to 24 weeks, using the Drooling Quotient (DQ), the Teacher Drooling Scale (TDS), and Visual Analog Scales (VAS). Results. Drooling was reduced during scopolamine application as well as after BoNT injections. Compared with baseline, the mean DQ showed a significant decrease throughout the study. Greatest reductions were achieved 2 to 8 weeks after BoNT injection. No significant differences were found between scopolamine measurements and those up to 24 months after BoNT injection. Using VAS, parents recorded the effect on drooling in which significant differences were found between baseline VAS score and all follow-up assessments. According to our definition of “success to therapy,” demanding a 2-point decrease on the TDS, 61.5% of patients responded to BoNT injections. Analysis of the DQ demonstrated a response rate of 53% of the patients to scopolamine and 48.7% to BoNT until 24 weeks after BoNT injections, the actual duration of this study. As a reaction to scopolamine, 71.1% of the patients had moderate to severe side effects. Only nonsevere, incidental side effects were reported after BoNT injections. Conclusions. During scopolamine application as well as after intraglandular BoNT injections, a clinically relevant reduction in drooling was achieved in children with CP, demonstrating maximum effect 2 to 8 weeks after injections. This is the first controlled clinical trial that confirmed a significant effect of BoNT injections in the treatment of drooling. General anesthesia was needed for all children. BoNT injections show fewer and less serious side effects than transdermal scopolamine treatment.
Shear stress-related mechanosignaling with lung ischemia: lessons from basic research can inform lung transplantation.

Cessation of blood flow represents a physical event that is sensed by the pulmonary endothelium leading to a signaling cascade that has been termed "mechanotransduction. " This paradigm has clinical relevance for conditions such as pulmonary embolism, lung bypass surgery, and organ procurement and storage during lung transplantation. On the basis of our findings with stop of flow, we postulate that normal blood flow is "sensed" by the endothelium by virtue of its location at the interface of the blood and vessel wall and that this signal is necessary to maintain the endothelial cell membrane potential. Stop of flow is sensed by a "mechanosome" consisting of PECAM-VEGF receptor-VE cadherin that is located in the endothelial cell caveolae. Activation of the mechanosome results in endothelial cell membrane depolarization that in turn leads to activation of NADPH oxidase (NOX2) to generate reactive oxygen species (ROS). Endothelial depolarization additionally results in opening of T-type voltage-gated Ca(2+) channels, increased intracellular Ca(2+), and activation of nitric oxide (NO) synthase with resultant generation of NO. Increased NO causes vasodilatation whereas ROS provide a signal for neovascularization; however, with lung transplantation overproduction of ROS and NO can cause oxidative injury and/or activation of proteins that drive inflammation and cell death. Understanding the key events in the mechanosignaling cascade has important lessons for the design of strategies or interventions that may reduce injury during storage of donor lungs with the goal to increase the availability of lungs suitable for donation and thus improving access to lung transplantation.
MisR/MisS Two-Component Regulon in Neisseria meningitidis

ABSTRACT Two-component regulatory systems are involved in processes important for bacterial pathogenesis. Inactivation of the misR/misS system in Neisseria meningitidis results in the loss of phosphorylation of the lipooligosaccharide inner core and causes attenuation in a mouse model of meningococcal infection. One hundred seventeen (78 up-regulated and 39 down-regulated) potential regulatory targets of the MisR/MisS (MisR/S) system were identified by transcriptional profiling of the NMBmisR mutant and the parental wild-type meningococcal strain NMB. The regulatory effect was further confirmed in a subset of target genes by quantitative real-time PCR and β-galactosidase transcriptional fusion reporter assays. The MisR regulon includes genes encoding proteins necessary for protein folding in the bacterial cytoplasm and periplasm, transcriptional regulation, metabolism, iron assimilation, and type I protein transport. Mutation in the MisR/S system caused increased sensitivity to oxidative stress and also resulted in decreased susceptibility to complement-mediated killing by normal human serum. To identify the direct targets of MisR regulation, electrophoretic mobility shift assays were carried out using purified MisR-His6 protein. Among 22 genes examined, misR directly interacted with 14 promoter regions. Six promoters were further investigated by DNase I protection assays, and a MisR-binding consensus sequence was proposed. Thus, the direct regulatory targets of MisR and the minimal regulon of the meningococcal MisR/S two-component signal transduction system were characterized. These data indicate that the MisR/S system influences a wide range of biological functions in N. meningitidis either directly or via intermediate regulators.
AIDIA - Adaptive Interface for Display InterAction

This paper presents a vision-based system for interaction with a display via hand pointing. An attention mechanism based on face and hand detection allows users in the camera’s field of view to take control of th e interface. Face recognition is used for identification and customisation. T he system allows the user to control the screen pointer by tracking their fist. On-screen items can be selected using one of four activation mechanisms. Current sample applications include browsing image and video collections as well as viewing a gallery of 3D objects. In experiments we demonstrate the performance of the vision components in challenging conditions and compare it to that of other systems.
Interferon-γ Release Assays for Diagnosis of Tuberculosis Infection and Disease in Children

Tuberculosis (TB) remains an important problem among children in the United States and throughout the world. Although diagnosis and treatment of infection with Mycobacterium tuberculosis (also referred to as latent tuberculosis infection [LTBI] or TB infection) remain the lynchpins of TB prevention, there is no diagnostic reference standard for LTBI. The tuberculin skin test (TST) has many limitations, including difficulty in administration and interpretation, the need for a return visit by the patient, and false-positive results caused by significant cross-reaction with Mycobacterium bovis–bacille Calmette-Guérin (BCG) vaccines and many nontuberculous mycobacteria. Interferon-γ release assays (IGRAs) are blood tests that measure ex vivo T-lymphocyte release of interferon-γ after stimulation by antigens specific for M tuberculosis. Because these antigens are not found on M bovis–BCG or most nontuberculous mycobacteria, IGRAs are more specific tests than the TST, yielding fewer false-positive results. However, IGRAs have little advantage over the TST in sensitivity, and both methods have reduced sensitivity in immunocompromised children, including children with severe TB disease. Both methods have a higher positive predictive value when applied to children with risk factors for LTBI. Unfortunately, neither method distinguishes between TB infection and TB disease. The objective of this technical report is to review what IGRAs are most useful for: (1) increasing test specificity in children who have received a BCG vaccine and may have a false-positive TST result; (2) using with the TST to increase sensitivity for finding LTBI in patients at high risk of developing progression from LTBI to disease; and (3) helping to diagnose TB disease.
Spatio-temporal Processing for Wireless Communications Spatio-temporal Processing for Wireless Communications

The use of base station antenna arrays in a wireless network can allow a more eecient use of the spatial dimension. Multiple antennas can provide processing and diversity gain to increase the base station range and improve coverage. Co-channel interference can be rejected spatially or by joint spatial and temporal processing of the multi channel signals. In addition, it is possible to employ spatially selective transmission, reducing co-channel interference on the forward link. This allows an increase in the capacity of the system. A wide range of wireless communication systems may beneet from spatial processing including high mobility cellular systems, low mobility short range systems, wireless local loop applications, satellite communications and wireless LAN. Herein, we discuss two channel modeling approaches. One quite general model which is useful for low mobility TDD systems and is well suited for joint spatio-temporal processing. An alternative channel model described by physical parameters is also discussed. This model is applicable in high-mobility FDD systems where macro cells are used. In this case, decoupled spatial and temporal processing can provide good interference rejection on both links.
Hawking radiation and masses in generalized dilaton theories

A generalized dilaton action is considered of which the standard dilaton black hole and spherically reduced gravity are particular cases. The Arnowitt - Deser - Misner (ADM) and the Bondi - Sachs (BS) mass are calculated. Special attention is paid to both the asymptotic conditions for the metric as well as for the reference spacetime. For the latter one we suggest a modified expression thereby obtaining a new definition of energy. Depending on the parameters of the model the Hawking radiation behaves like a positive or negative power of the mass.
Management of premenstrual syndrome

©FSRH J Fam Plann Reprod Health Care 2009: 35(3) Introduction The diagnosis and management of premenstrual syndrome (PMS) are reviewed, in particular, the evidence for pharmacological and non-pharmacological treatments. Additionally, this review takes a wider view of the situation with particular emphasis on recent attempts to achieve a global consensus on definitions and management. The development of a consensus and guidelines on the definition and management of PMS is essential to encourage acceptance of this condition by patients, health professionals and regulatory authorities.