Miracle-dz/UniFAR-FTUs huggingface dataset:
Distribution of potassium ion and water permeable channels at perivascular glia in brain and retina of the Largemyd mouse

The dystroglycan protein complex provides a link between the cytoskeleton and the extracellular matrix (ECM). Defective O‐glycosylation of α‐dystroglycan (α‐DG) severs this link leading to muscular dystrophies named dystroglycanopathies. These are characterized not only by muscle degeneration, but also by brain and ocular defects. In brain and retina, α‐DG and ECM molecules are enriched around blood vessels where they may be involved in localizing the inwardly rectifying potassium channel, Kir4.1, and aquaporin channel, AQP4, to astrocytic endfeet. To investigate in vivo the role of ECM ligand‐binding to glycosylated sites on α‐DG in the polarized distribution of these channels, we used the Largemyd mouse, an animal model for dystroglycanopathies. We found that Kir4.1 and AQP4 are lost from astrocytic endfeet in brain whereas significant labeling for these channels is detected at similar cell domains in retina. Furthermore, while both α‐ and β1‐syntrophins are lost from perivascular astrocytes in brain, labeling for β1‐syntrophin is found in retina of the Largemyd mouse. These findings show that while ligand‐binding to the highly glycosylated isoform of α‐DG in concert with α‐ and β1‐syntrophins is crucial for the polarized distribution of Kir4.1 and AQP4 to functional domains in brain, distinct mechanisms may contribute to their localization in retina.
Herland—An All-female Women's Utopia

Charlotte Gilman’s utopian masterpiece Herland (1915) dramatizes a confrontation between three men and an all-female society. Gilman not only creates a political vacuum, where the whole patriarchal civilization, including patriarchal system, superstructure, ideology, influence, and consciousness have ceased to exist, but also men are done away with all together. Most reviews claim that Herland criticizes the patriarchal tradition and manifests concern for humanity and some even regard Herland as the first truly feminist work in the American tradition. But this supposedly utopian world is actually static, without possibilities of growth and even inhuman, gothic, and nightmarish. And this is because Gilman constructs the women’s utopia out of the conviction in women’s superiority over men. Herland is a little paradise that is designed too perfect. Women’s utopias still need to promote social change in the real world.
Tourists as Story-Builders: Narrative Construction at a Heritage Museum

ABSTRACT Although tourism scholarship supports the idea that the appreciation of a tourism experience depends on the availability and communication of stories, attention has been paid almost exclusively on marketers and tourism organizers as the main designers and promoters of narratives, ignoring the role of tourists themselves in this process. Using heritage museums as an empirical context, this research suggests that the success of a narrative presentation in tourism destinations also depends on the tourists' involvement, willingness, and ability to actively participate in the storytelling experience. In this sense, tourists act as story-builders whose main task is to construct a historical narrative based on the selection and integration of the available sources. The findings show that museum visitors go through a three-stage process during their effort to complete a heritage narrative and make sense of their tourism experience. This work sheds light on the coconstruction of tourism experiences and provides marketing direction for the successful narrative staging of museums and tourism places in general.
Transendothelial Movement of Liposomes In Vitro Mediated by Cancer Cells, Neutrophils or Histamine

A two‐chamber culture system has been used to examine the ability of small liposomes to cross an endothelial cell barrier in response to various stimuli. Transendothelial transit of liposomes was almost negligible in the presence of intact, healthy endothelial cells (EC). Addition of histamine induced a concentration‐dependent increase in the movement of liposomes across the EC monolayer. In the presence of polymorphonuclear neutrophils (PMNs), migrating in response to a chemotactic gradient of N‐Formil‐Met‐Leu‐Phe (fMLP), both liposomes and IgG crossed EC monolayer by a paracellular pathway, largely independent of an association with the PMNs. The presence of cancer cell, growing in the lower chamber or the presence of cancer cell‐conditioned media, also resulted in the passage of liposome across the EC. We conclude that EC monolayers are sufficiently disrupted by several physiologically relevant stimuli to allow for the transendothelial passage of liposomes. These results have important implications for the therapeutic use of liposome in the treatment of cancer or other inflammatory processes.
Brecht reads Bruegel: Verfremdung, Gestic Realism and the Second Phase of Brechtian Theory

Brecht’s interest in Bruegel is widely testified, but no one has asked quite what the playwright and theorist saw in the pictures of the Flemish master. This article tracks Brecht’s reception, from a putative first encounter in 1936 and through his own notes on the pictures, and demonstrates how closely Brecht’s reading of Bruegel may have entwined with his contemporary reflections on Verfremdung, Gestus and “cognitive realism,” as also with the theatre projects of the later exile period. There are particularly close relationships, for example, between Bruegel’s Dulle Griet and Mutter Courage and Der kaukasische Kreidekreis. Through the gaze of the modernist dramatist, Bruegel comes across as an artist of political cunning whose works demand a complex (even dialectic) cognitive process in order to read and decipher them. He is, like Brecht, both a satirist and a realist. His paintings are full of inversions designed to unsettle the onlooker’s conventional views, full of political hints and separate little narrative episodes to tease. What Brecht discovered in Bruegel and in the detail of Bruegel’s pictures was to have far-reaching consequences, both for the formulation of his theory and for the look and practice of his theatre. (TK)
Uromodulin Biology and Pathophysiology – An Update

Uromodulin (UMOD) is a glycoprotein expressed on the luminal surface of the apical membrane of renal tubular epithelial cells forming the thick ascending limb of Henle. Here, UMOD forms filamentous structures probably ensuring water impermeability and the countercurrent gradient. The multidomain structure, cellular topology of UMOD and clinical consequences associated with UMOD dysfunction, however, suggest that it may be involved in other biological processes such as receptor-mediated endocytosis, mechanosensation of urinary flow, Wnt-signaling, cell cycle regulation and planar cell polarity. A specific, but as yet unidentified, protease(s) releases UMOD into the urine, where it probably contributes to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and modulates formation of supersaturated salts and their crystals. UMOD expression, biosynthesis and excretion are regulated in a complex manner, and dysregulation is found in a wide range of pathological conditions. It is strongly reduced or absent in cases with mutations in UMOD, renin, HNF1B and other genetic disorders causing autosomal dominant hyperuricemic nephropathy. In contrast, elevated UMOD excretion may be associated with, and thus predictive of, chronic kidney disease. UMOD analysis is therefore of importance in all conditions with renal involvement and may be useful in the proper classification of renal diseases.
Identification of Novel Kaposi's Sarcoma-Associated Herpesvirus Orf50 Transcripts: Discovery of New RTA Isoforms with Variable Transactivation Potential

ABSTRACT Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus that has been associated with primary effusion lymphoma and multicentric Castleman's disease, as well as its namesake Kaposi's sarcoma. As a gammaherpesvirus, KSHV is able to acutely replicate, enter latency, and reactivate from this latent state. A key protein involved in both acute replication and reactivation from latency is the replication and transcriptional activator (RTA) encoded by the gene Orf50. RTA is a known transactivator of multiple viral genes, allowing it to control the switch between latency and virus replication. We report here the identification of six alternatively spliced Orf50 transcripts that are generated from four distinct promoters. These newly identified promoters are shown to be transcriptionally active in 293T (embryonic kidney), Vero (African-green monkey kidney epithelial), 3T12 (mouse fibroblast), and RAW 264.7 (mouse macrophage) cell lines. Notably, the newly identified Orf50 transcripts are predicted to encode four different isoforms of the RTA which differ by 6 to 10 residues at the amino terminus of the protein. We show the global viral transactivation potential of all four RTA isoforms and demonstrate that all isoforms can transcriptionally activate an array of KSHV promoters to various levels. The pattern of transcriptional activation appears to support a transcriptional interference model within the Orf50 region, where silencing of previously expressed isoforms by transcription initiation from upstream Orf50 promoters has the potential to modulate the pattern of viral gene activation. IMPORTANCE Gammaherpesviruses are associated with the development of lymphomas and lymphoproliferative diseases, as well as several other types of cancer. The human gammaherpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV), is tightly associated with the development of Kaposi's sarcoma and multicentric Castleman's disease, as well as a rare form of B cell lymphoma (primary effusion lymphoma) primarily observed in HIV-infected individuals. RTA is an essential viral gene product involved in the initiation of gammaherpesvirus replication and is conserved among all known gammaherpesviruses. We show here for KSHV that transcription of the gene encoding RTA is complex and leads to the expression of several isoforms of RTA with distinct functions. This observed complexity in KSHV RTA expression and function likely plays a critical role in the regulation of downstream viral and cellular gene expression, leading to the efficient production of mature virions.
Environment and Planning B: Planning and Design

In a recent paper Czamanski and Roth (2011) demonstrated that because the profitability of construction projects is influenced by variations in the time incidence of costs and revenues, despite of declining willingness-to-pay and land gradients with distance from central business districts, profitability can experience local maxima away from urban centers. The time until the realization of revenues was termed “characteristic time”. Its size is the result of planning polices and can lead to leapfrogging and scattered development, especially when interest rates are low or negligible. We explained this result by modeling the simple behavior of developers in the context of a single linear city. In this paper we consider the case of two municipalities with different development policies and characteristic time functions. We explore local maxima in profitability, typical of disequilibrium situations, especially during periods that cities are growing. Myopic assumptions, in the sense that each city is interested only in what happens on its side of the municipal boundary, can easily lead to unintended leapfrogging. Competition between the cities can result in intentional leapfrogging or in spatially concentrated development, depending on the policy objectives. We extend the analysis further and consider qualitatively different cities that give rise to different gravity-type forces and differences in willingness to pay. The demand and supply sides of the building market are integrated into the model. The additional considerations can lead to various patterns of scattered development capable of explaining the spatial structure of metropolitan areas. 1 The authors wish to thank the Israel Science Foundation for its generous support of this research.
Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives

Spore-forming bacilli are being explored for the production and preservation of food for many centuries. The inherent ability of production of large number of secretory proteins, enzymes, antimicrobial compounds, vitamins, and carotenoids specifies the importance of bacilli in food chain. Additionally, Bacillus spp. are gaining interest in human health related functional food research coupled with their enhanced tolerance and survivability under hostile environment of gastrointestinal tract. Besides, bacilli are more stable during processing and storage of food and pharmaceutical preparations, making them more suitable candidate for health promoting formulations. Further, Bacillus strains also possess biotherapeutic potential which is connected with their ability to interact with the internal milieu of the host by producing variety of antimicrobial peptides and small extracellular effector molecules. Nonetheless, with proposed scientific evidences, commercial probiotic supplements, and functional foods comprising of Bacillus spp. had not gained much credential in general population, since the debate over probiotic vs pathogen tag of Bacillus in the research and production terrains is confusing consumers. Hence, it’s important to clearly understand the phenotypic and genotypic characteristics of selective beneficial Bacillus spp. and their substantiation with those having GRAS status, to reach a consensus over the same. This review highlights the probiotic candidature of spore forming Bacillus spp. and presents an overview of the proposed health benefits, including application in food and pharmaceutical industry. Moreover, the growing need to evaluate the safety of individual Bacillus strains as well as species on a case by case basis and necessity of more profound analysis for the selection and identification of Bacillus probiotic candidates are also taken into consideration.
Developmental toxicity of orally administered technical dimethoate in rats.

BACKGROUND Dimethoate (O,O-dimethyl-S-(N-methylcarbamoyl-methyl) phosphorodithioate), an organophosphate insecticide, was examined for its potential to produce developmental toxicity in rats after oral administration. METHODS Pregnant Fischer 344 rats were given sublethal doses of 0 (corn oil), 7, 15, and 28 mg/kg/day dimethoate by gavage on gestation days (GD) 6-15. Maternal effects in 15 and 28 mg/kg/day dose groups included cholinergic signs such as tremors, diarrhea, weakness, and salivation, and depression in the maternal and fetal brain acetylcholinesterase (AChE) activities. Other maternal toxicity that included reduction in body weight and feed consumption was observed only in the treated group of 28 mg/kg/day. No maternal toxicity was apparent in the 7 mg/kg/day dose group. RESULTS Maternal exposure to dimethoate during organogenesis significantly affected the number of live fetuses, early resorption, and mean fetal weight in the 28 mg/kg/day dose group. No external, visceral, and skeletal abnormalities were observed in any of the treated groups compared to the control. CONCLUSIONS On the basis of the present results dimethoate can produce clinical signs of toxicity and significant inhibition of the maternal and fetal AChE activities in dose groups of 15 and 28 mg/kg/day and showed fetotoxicity without teratogenic effects at 28 mg/kg/day.
A comparative study on manufacturing strategy of Chinese and German manufacturer

Globalization leads to the movement of companies towards international cooperation in manufacturing. Manufacturing strategy (MS) is one of the most important issues for manufacturing companies to secure sustainable advantage. It is imperative for companies to understand the manufacturing strategy of their international cooperators to ensure cooperation success. In the last decades, more and more Sino-German cooperative Manufacturing has been occurred. China differs from Germany in a number of aspects such as economy, institution and social culture. Based on an empirical study, this paper compares the manufacturing strategies in relation to the manufacturing objective and practice between Chinese and Germany industry and the background for the differences is discussed.
Expiration-Day Effects and the Impact of Short Trading Breaks on Intraday Volatility: Evidence from the Indian Market

One distinct feature of the Indian stock market is the large trading volume of single stock futures, which are cash settled on the basis of the volume-weighted average spot prices of the underlying stocks during the last half-an-hour of trading on the expiration day. We investigate the expiration day effect on intraday volatility and find that the volatility of the stocks increases in the last half-an-hour trade on the expiry day but not during the other time intervals. We also investigate the volatility surrounding the intraday trading breaks induced by satellite communication outages, a peculiar feature of the Indian stock market till 2008, and find that the volatility rises when the market reopens after the breaks but not before the breaks. © 2013 Wiley Periodicals, Inc. Jrl Fut Mark
Firm debt structure, firm size and risk volatility in US industrial firms

US industrial-firm panel data on short-term and long-term borrowing (term debt structure) for annual and quarterly time periods over the years 1995 to 2008 are used to test an insulation hypothesis and a related volatility hypothesis. The former test uses a regression model relating the log of the ratio of accounts payable in trade to Long-Term Debt (LTD) to firm size and other variables. The focus is on the firm's response to the US Federal Reserve (FED)'s monetary policy, where the response is a micro perspective on the earlier macro debate over the existence of bank lending channels. The latter hypothesis uses the panel heteroscedastic variances from the first regression procedure to test for a quadratic-form risk function (either U-shaped or inverted U-shaped) using sigma squared and the Coefficient of Variation (CV) as risk indexes and firm size as a determinant. The findings suggest that there is some evidence that US industrial firms in their borrowing behaviour do insulate themselves from the effects of monetary policy and that retained earnings have a significant role in the insulation effect. The evidence also suggests that the risk index, the net variances of the debt ratio, is related to firm size by a U-shaped quadratic function with most of the actual observations on the downward sloping part of the function. As firm size increases, not only does the term-structure ratio fall, but also the volatility falls and at a falling rate of change, approaching zero for a sufficiently large firm.
Radiographic evaluation of dental age maturity in 3–17-years-old saudi children as an indicator of chronological age

Objective: This study was aimed to evaluate the dental age in Saudi children from panoramic radiographs using the Demirjian method to estimate their chronological age. Materials And Methods: This retrospective cross-sectional study consisted of 1902 panoramic radiographs of 955 boys and 947 girls between the ages of 3–17 years. All children were placed in the age group closest to their chronological age. The dental age was scored on all seven left mandibular teeth by calibrated examiners. Bivariate analyses using the t-test and Pearson correlation were performed. Results: There was significant difference in both boys and girls in all the age groups between their chronological age and dental age. Even though there was a slight overestimation in boys in some age groups and slight underestimation in girls in some groups, correlation analysis showed that there was a highly significant correlation between the chronological age and dental age for both boys (r2 = 0.96, P < 0.001) and girls (r2 = 0.98, P < 0.001). Moreover, correlation analyses for each age group showed a significant correlation between the chronological age and the dental age, using the Demirjian method, in most age groups (P < 0.01). When comparing the maturation score between boys and girls, the Student's t-test showed that there were no statistical differences between boys and girls in most age groups. Conclusion: Saudi boys and girls living in the western region of Saudi Arabia exhibited similar pattern of dental development when compared to the Demirjian method. Hence, the Demirjian method could be used as reference in children from the western region of Saudi Arabia.
Impact of Information Systems (IS) Steering Committees on IS Planning

One of the most efficient ways to manage the information systems function is to introduce steering committees. It has been suggested that one of the foremost benefits of a steering committee is that it can improve the information systems planning efforts of an organization. However, there is very little empirical evidence in the literature that deals with how such committees affect planning. This study investigates the impact of steering committees on three phases of information systems planning: strategic planning, systems planning, and implementation of planning. The greatest impact of steering committees appears to be the strategic planning phase, while a lesser effect is observed in the systems planning and implementation of planning phases.
THE END OF THE EXTERNALITY REVOLUTION

In the early 1970s, we and others in the economics profession became enamored with the notion of externalties—a cost or benefit imposed on or provided to others but not taken into account by the economic agents who generate the effect. We, and others, seemed to see external effects everywhere. There was polluted water and air, noise, urban blight, traffic congestion, and other features of modern life that seemed to call out for some form of corrective action. As the externalities revolution unfolded, economists and other social scientists overlooked the importance of evolved legal and other institutions that formally and informally establish property and liability rules that cause decision makers to face the cost of their actions, including what otherwise could be external costs imposed on unwilling third parties. While markets seemed always to fail, political institutions were seen systematically as without blemish, or so it seemed. It was this two-pronged failure, 1) a failure to consider and state assumptions about background institutional arrangements and 2) a disregard for special interest politics, that became the Achilles Heel of the otherwise elegant externality arguments. Eventually, it was the modern institutionalists, scholars who focused on laws, regulation, and rules of the marketplace, who attempted to close the lid and drive the nails on the externality coffin. In this paper, we reach back to 1920 and trace the rise and decline of the policy importance of externalities theory. Beginning with A. C. Pigou and Alfred Marshall, our story includes some of the great figures in economic history of thought. But while theory was being built, institutions were overlooked. Pigou continues to be a dominant player in the story until the 1960s and 1970s when externalities theory was challenged by James M. Buchanan, Ronald Coase and other scholars. It is here in the twilight years of the externalities revolution that the prospects of government failure are raised as being more daunting than the likelihood of market failure. Finally, in the late 1970s and beyond, the externalities revolution is replaced by a property rights revolution.