Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of two gene loci involved in poly-beta-hydroxybutyrate production in Rhodobacter sphaeroides FJ1.

BACKGROUND AND PURPOSE Polyhydroxyalkanoates (PHAs), biopolyesters of hydroxyl fatty acids, are synthesized and deposited as cytoplasmic inclusions in many bacteria. We isolated a poly-beta-hydroxybutyrate (PHB)-producing bacterium designated Rhodobacter sphaeroides FJ1. To characterize PHB biosynthesis in this organism, we isolated the genes encoding proteins involved in PHB metabolism. METHODS The genes responsible for the synthesis, accumulation, and degradation of PHB in R. sphaeroides FJ1 were cloned and characterized. RESULTS Genes involved in the biosynthesis and metabolism of PHB were found to be located in 2 different loci in the genome of R. sphaeroides FJ1. One locus contained genes encoding PHB depolymerase (phbZ), PHB synthase (phbC), phasin (phbP) and the regulator protein (phbR). The other locus contained the beta-ketothiolase gene (phbA) and the acetoactyl-CoA reductase gene (phbB). The phbZ gene was orientated in an opposite direction to that of phbC, phbP and phbR genes that were located in the same cluster. R. sphaeroides FJ1 was able to grow in wastewater released from the human waste treatment plant of Fu-Jen University. Optimal growth and PHB production were achieved when R. sphaeroides FJ1 was grown in tryptic soy broth containing 50% wastewater. PHB production by R. sphaeroides FJ1 varied in media with different carbon to nitrogen ratios, but the level of PHB synthase was constant, suggesting that PHB production depends mainly on substrate supply. CONCLUSIONS Six genes encoding proteins related to PHB metabolism are clustered in 2 separate loci, phbZCPR and phbAB, in a PHB-producing bacterium R. sphaeroides FJ1 isolated from wastewater. PHB synthase, the key enzyme for PHB synthesis, is constitutively expressed, and its expression level is not affected by different growth conditions.
Incidence of epithelial ovarian cancer according to histologic subtypes in Korea, 1999 to 2012

Objective To investigate trends in the incidence of epithelial ovarian cancer (EOC), according to histologic subtypes, in Korean women between 1999 and 2012. Methods Data from the Korea Central Cancer Registry recorded between 1999 and 2012 were evaluated. The incidences of EOC histologic subtypes were counted. Age-standardized incidence rates (ASRs) and annual percentage changes (APCs) in incidence rates were calculated. Patient data were divided into three groups based on age (<40, 40 to 59, and >59 years), and age-specific incidence rates were compared. Results Overall, the incidence of EOC has increased. Annual EOC cases increased from 922 in 1999 to 1,775 in 2012. In 1999, the ASR was 3.52 per 100,000 and increased to 4.79 per 100,000 in 2012 (APC, 2.53%; p<0.001). The ASRs in 2012 and APCs between 1999 and 2012 for the four major histologic subtypes were as follows (in order of incidence): serous carcinoma (ASR, 2.32 per 100,000; APC, 4.34%; p<0.001), mucinous carcinoma (ASR, 0.73 per 100,000; APC, –1.05%; p=0.131), endometrioid carcinoma (ASR, 0.51 per 100,000; APC, 1.48%; p=0.032), and clear cell carcinoma (ASR, 0.50 per 100,000; APC, 8.13%; p<0.001). In the sub-analyses based on age, clear cell carcinoma was confirmed as the histologic subtype whose incidence had increased the most since 1999. Conclusion The incidence of EOC is increasing in Korea. Among the histologic subtypes, the incidence of clear cell carcinoma has increased markedly across all age groups since 1999.
Gender, Internal Controls, and Academic Dishonesty: Investigating Mediating and Differential Effects

While research has found that males tend to commit more academically dishonest acts than females, we know little about what accounts for the gender differential or whether the same factors can explain academic dishonesty for males and females. This study assesses how internal controls, i.e., self‐control, shame, embarrassment, and moral beliefs, account for the relationship between gender and cheating behavior. Using a sample of students from a southern university in the United States, this study explores three questions. First, do internal controls vary across males and females? Second, can internal controls account for gender differences in test cheating? Third, do internal controls differentially or similarly predict test cheating for males and females? Results show that (a) gender differences among several internal controls as well as cheating behavior, (b) internal controls reduce the gender gap but cannot completely explain why gender differences exist in cheating, and (c) two internal controls, self‐control and moral beliefs, differentially predict male and female test cheating. We discuss limitations of our findings and how research can expand the investigation of gender differences and explanations for engaging in academically dishonest acts.
Dysfunctional mitochondrial respiration in the striatum of the Huntington’s disease transgenic R6/2 mouse model

Metabolic dysfunction and mitochondrial involvement are recognised as part of the pathology in Huntington's Disease (HD). Post-mortem examinations of the striatum from end-stage HD patients have shown a decrease in the in vitro activity of complexes II, III and IV of the electron transport system (ETS). In different models of HD, evidence of enzyme defects have been reported in complex II and complex IV using enzyme assays. However, such assays are highly variable and results have been inconsistent. We investigated the integrated ETS function ex vivo using a sensitive high-resolution respirometric (HRR) method. The O2 flux in a whole-cell sample combined with the addition of mitochondrial substrates, uncouplers and inhibitors enabled us to accurately quantitate the function of individual mitochondrial complexes in intact mitochondria, while retaining mitochondrial regulation and compensatory mechanisms. We used HRR to examine the mitochondrial function in striata from 12-week old R6/2 mice expressing exon 1 of human HTT with 130 CAG repeats. A significant reduction in complex II and complex IV flux control ratios was found in the R6/2 mouse striatum at 12 weeks of age compared to controls, confirming previous findings obtained with spectrophotometric enzyme assays.
Social Support in Older Age: Assessing the Impact of Marital and Parent Status

RÉSUMÉ Dans le cadre de cette étude, nous examinons les répercussions de la situation de parent et de l'état matrimonial sur (1) les fondations des réseaux de soutien sociaux et (2) l'identité des personnes auxquelles on fait appel afin d'obtenir de l'aide pour accomplir ses activités quotidiennes. Les données proviennent d'entrevues structurées menées auprès de 678 personnes de 55 ans et plus vivant à leur domicile à London, en Ontario. Aux fins de l'analyse, cet échantillon compte une surreprésentation des personnes sans enfant, des célibataires (personnes jamais mariées) et des personnes divorcées afin d'assurer un nombre adéquat dans chaque catégorie. Nos résultats ont démontré que l'état matrimonial a une plus grande influence que la situation de parent en ce qui touche les différences dans les fondations du réseau de soutien social. Toutefois, dans le cas des formes d'assistance plus spécialisées, les personnes divorcées et les célibataires sont plus susceptibles que les personnes mariées, et les personnes sans enfant que les parents, de faire appel aux services de soutien formels et aux services d'aide professionnels, et moins portés à se reposer sur la famille. De plus, tant dans le cas de l'état matrimonial que dans la situation de parent, les résultats démontrent une importante interaction entre les sexes. ABSTRACT In this study we examine the impact of parent status and marital status on (1) the foundations of social support networks and (2) who one turns to if help is required with activities of daily living. Data are taken from structured interviews with 678 community-dwelling persons aged 55 and over in London, Canada. This sample overrepresents the childless, single (never married), and divorced, thus ensuring adequate numbers in each category for data analysis. Our findings indicate that marital status is more influential than parent status regarding differences in the foundations of social support. However, for more specific forms of assistance, the divorced and single are more likely than the married, and the childless are more likely than parents to rely on formal support and paid help and less likely to rely on family. As well, for both marital and parent status, there are significant interactions with gender.
Strength and power changes of the human plantar flexors and knee extensors in response to resistance training in old age.

AIM The aim of the present study was to assess and compare the improvements of muscle strength and power induced by a 16-week resistive programme in a population of 16 older men aged 65-81 years. METHODS Training was performed three times per week at an intensity of 80% of one repetition maximum (1RM) and consisted of both calf raise and leg press exercises. Before-, during- and after-training, maximum isometric and isokinetic torques, maximum power, 1RM, muscle cross-sectional area (CSA) and electromyographic activity (EMG) of the plantar flexors (PF) and knee extensors (KE) were examined. RESULTS For the KE and PF, respectively, training resulted in a 29.9 +/- 4.4% (mean +/- SE) and 21.6 +/- 5.4% increase in 1RM (P < 0.001-0.01), a 19.4 +/- 4.3 and 12.4 +/- 4.7% (P < 0.001-0.05) increase in maximum isometric torque, and a 24.1 +/- 6.3 and 33.1 +/- 10.9% (P < 0.05) increase in maximum muscle power, calculated from torque-angular velocity curves. The large increase in torque and power was partly accounted by a significant increase in the CSA of the PF (5.0 +/- 0.7%) and KE (7.4 +/- 0.7%), while no significant changes in integrated EMG activity of vastus lateralis and soleus muscles, and in extrapolated maximum shortening velocity were found. After training, a significant increase in torque/CSA (10.3 +/- 4%, P < 0.05) was found for the KE but not for the PF. CONCLUSION Hence, hypertrophy cannot alone justify the increase in torque, and other factors, such as an increase in individual fibre-specific tension (in the case of KE), a decrease in antagonist muscles' coactivation, an improved co-ordination and an increased neural drive of the other heads of quadriceps may have contributed to the increments in strength. The significant increase in muscle power seems particularly noteworthy with respect to daily activities involving the displacement of the body over time, namely, the generation of muscle power.
The Effect of School Racial Composition on Hispanic Intergroup Relations

The present study examined how intergroup attitudes and behavior among Hispanic middle school students varied as a function of school racial/ethniccomposition. Data were derived from a survey of all students present in thesixth to eighth grades of three public middle schools (N= 1,837). The schools represented three distinctive racial compositions: Hispanic/Black, Hispanic/White, and Hispanic majority. Attitudes, as measured by other-group orientation, varied across the three school settings, but not behavior, as measured by reported number of cross-race close friendships. Differences between the Hispanic/Black and Hispanic/White schools in perceived comfort of close friends with cross-race social interaction suggested a social norms or cultural distance interpretation of the results. The revised contact hypothesis was partially supported in that Hispanic students’ other-group orientation was predicted by both school racial proportion and reported number of cross-race close friends; however, contrary to theory, cross-race friendships did not contribute more of the variance. A key finding of the study was the importance of perceived comfort level of close friends with cross-race social interaction as a predictor of other-group orientation.The study offers preliminary evidence that there may be important differences in the intergroup relations of Hispanic students in multi-ethnic schoolswhen these are compared with previous studies of Black/White relations.
How (not What) Shall We Think about Human Rights and Religious Arguments? Public Reasoning and Beyond

This paper addresses the question of how (not what) we should think about human rights and religious arguments. Thinking about this relationship is today particularly important, because conflicts over human rights in practice often turn around their theoretical problems. Should religious arguments be used to justify human rights? Or do we want human rights to be free from any partisan endorsement so as to avoid divisive interpretations of universal principles? Underlying these hard questions is the issue of justification in view of a plurality of cultural and religious traditions around the globe. If human rights can be transformed so as to defy the charge of Euro-centrism (of being parochially rooted in only one cultural and religious tradition), they need to creatively draw on, not pit themselves against, this plurality. This paper suggests a framework for such a positive and inclusive engagement with various cultures and religions that goes beyond the mainstream liberal model of “public reason”.
Escala de bienestar de Ryff: análisis comparativo de los modelos teóricos en distintos grupos de edad

The scale of psychological well-being arises from the multidimensional model proposed by Ryff (1989). Despite its wide use, its initial theoretical structure has not been completely confirmed because other factorial solutions which are different from those 6 dimensions proposed by the author have been found. These differences may be related to the type of sample and population used. Given this fact, the current study compares the adjustment factor of correction models traditionally used in different groups of adults. We describe the psychometric properties of the Spanish adaptation done by Diaz et al. (2006) and analyze the levels of reliability (internal consistency and temporal stability) and confirmatory factorial structure with data from 1,646 people aged from 18 to 90 years old. Differences are found in the indicators of reliability for the total scale and its dimensions as well as in the indicators of goodness of fit depending on the age group. The model that presents the best indicators of adjustment for most age ranges evaluated was the one of six first-order factors.
Drosophila Immunity: Analysis of PGRP-SB1 Expression, Enzymatic Activity and Function

Peptidoglycan is an essential and specific component of the bacterial cell wall and therefore is an ideal recognition signature for the immune system. Peptidoglycan recognition proteins (PGRPs) are conserved from insects to mammals and able to bind PGN (non-catalytic PGRPs) and, in some cases, to efficiently degrade it (catalytic PGRPs). In Drosophila, several non-catalytic PGRPs function as selective peptidoglycan receptors upstream of the Toll and Imd pathways, the two major signalling cascades regulating the systemic production of antimicrobial peptides. Recognition PGRPs specifically activate the Toll pathway in response to Lys-type peptidoglycan found in most Gram-positive bacteria and the Imd pathway in response to DAP-type peptidoglycan encountered in Gram-positive bacilli-type bacteria and in Gram-negative bacteria. Catalytic PGRPs on the other hand can potentially reduce the level of immune activation by scavenging peptidoglycan. In accordance with this, PGRP-LB and PGRP-SC1A/B/2 have been shown to act as negative regulators of the Imd pathway. In this study, we report a biochemical and genetic analysis of PGRP-SB1, a catalytic PGRP. Our data show that PGRP-SB1 is abundantly secreted into the hemolymph following Imd pathway activation in the fat body, and exhibits an enzymatic activity towards DAP-type polymeric peptidoglycan. We have generated a PGRP-SB1/2 null mutant by homologous recombination, but its thorough phenotypic analysis did not reveal any immune function, suggesting a subtle role or redundancy of PGRP-SB1/2 with other molecules. Possible immune functions of PGRP-SB1 are discussed.
Hemodialysis nurses rate nursing diagnoses relevant to clinical practice

Pontifícia Universidade Católica de Goiás, Escola de Ciências Sociais e da Saúde, Goiás, Brazil. Universidade Federal de Goiás, Faculdade de Enfermagem, Goiás, Brazil. Conflicts of interest: there are no conflicts of interest to declare. Abstract Objectives: To assess the profile of nursing diagnoses rated by hemodialysis nurses as most relevant to clinical practice in the field. Methods: This was a descriptive cross-sectional study conducted from August to September 2014 in the city of Goiânia, Goiás, Brazil. A questionnaire and a Likert scale (score 0-7) were given to participants to assess the relevance of diagnoses. Scores were obtained by calculating the ratio of the total score obtained to the highest score possible. Diagnoses that scored ≥0.75 were considered relevant. Results: Forty nurses participated in the study, 80% had been working in the field for over 24 months, 42.5% were nephrology experts. The participants indicated 44 relevant nursing diagnoses, discussed in the context of hemodialysis nursing. Conclusion: The diagnoses identified show the human responses valued by hemodialysis nurses.
Bringing installation art to reconnaissance to share values and generate action

The English education system has recently seen something of a revival of enthusiasm for the use of research both to develop educational practices and to gather evidence about their effectiveness. These initiatives often present action research as a model of individual problem-solving, which, we argue, communicates a limited conception of action research. In this paper we propose an alternative to this ‘problem-solving’ conception of action research that acknowledges the complex, messy nature of action research through the use of arts installations. Specifically, we present the reconnaissance phase of a project which brought together a partnership comprising a water heritage museum, university staff, teachers and artists. A pedagogical adaptation of contemporary installation art theory and practice fostered the exploration of individual and collective understandings of water, and also established a shared approach to curriculum development and ownership of the project among all participants. We propose that this creative practice enhanced and changed the process of reconnaissance; it allowed the group to establish and share commitments to the value of water conservation and generated a wide range of options for our action research.
The Role of Knowledge, Social Norms, and Attitudes Toward Organic Products and Shopping Behavior: Survey Results from High School Students in Vienna

In 2005 a survey was used to investigate social norms and attitudes of Viennese high school students (14–20 years, n = 340) toward organic products. Young people, who already participate in household decisions and consume organic products, have not yet been recognized sufficiently in research. The Theory of Reasoned Action and discriminant analysis are used to explore relations of a number of variables and the complex field of factors influencing Viennese high school students’ shopping behavior when shopping for organic products. Key findings include the importance of primary socialization in forming social norms and shaping behavior. Surprisingly, knowledge of organic products does not explain students’ self-reported shopping behavior when shopping for organic products. Cultural patterns seem to be far more useful to predict behavior and attitudes toward organic products.
A Segment-Based Dynamic Programming Algorithm for Predicting Gene Structure

An algorithm called segment-based dynamic programming is described for predicting gene structure from a sequence of genomic DNA. The algorithm explores the space of gene structures that satisfy junctional and frame constraints and finds the gene structure that optimizes the sum of junctional and segmental scoring functions. Junctional constraints specify acceptable sites of initiation, termination, and splicing, whereas frame constraints ensure that the total exon length is a multiple of three and that no in-frame stop codons occur within exons or at exon-exon junctions. By computing over segments, segment-based dynamic programming maintains reading frame and phase information for each segment, it can assemble exons in-frame as well as score them in-frame. The algorithm is used to quantify the computational power of constraints. Experimental results show that frame constraints reduce the size of the search space by several orders of magnitude and that cardinality constraints place an asymptotic limit on the size of the search space. The algorithm is also used to compare the accuracy of different methods for assembly and scoring. A scoring scheme based on fifth-order Markov hexamer frequencies is presented and used in three objective functions, corresponding to in-frame, frame-independent, and frame-maximal scoring strategies. Experimental results show that in-frame assembly improves specificity only slightly over frame-independent assembly, whereas in-frame scoring improves specificity substantially over frame-independent and frame-maximal scoring.
Patterns of ambulatory care use for gynecologic conditions: A national study.

OBJECTIVE This study was undertaken to describe the site of ambulatory care visits for gynecologic conditions in the United States and to identify patient factors associated with the site of care for these conditions. STUDY DESIGN We conducted a national cross-sectional study using data from the 1995-1996 National Ambulatory Medical Care and National Hospital Ambulatory Medical Care Surveys. Visits to private physician offices, hospital outpatient departments, and emergency departments were selected if the principal diagnoses were consistent with 1 of 9 gynecologic categories. Multiple logistic regression was used for all diagnoses to identify factors associated with visits to emergency departments or hospital-based outpatient departments compared with factors associated with visits to private physician offices. Separate regression models were developed for individual diagnoses to test the hypothesis that the factors associated with the site of care would vary across different gynecologic conditions. RESULTS There were 23,194,000 visits for gynecologic conditions during the 2-year study period. Genital dysplasia, ovarian disorders, and uterine disorders were associated with greater use of hospital outpatient departments and emergency departments compared with physician offices. There was a 30% to 50% reduction in emergency room use for visits by women aged 45 years and older compared with visits by women aged 18 to 29 years. Emergency department use for several gynecologic conditions was 5 to 8 times greater for visits by women with household income <$29,000 than for visits by women with household income > or =$40,000. CONCLUSION Specific gynecologic diagnoses and patient factors are associated with greater use of emergency departments or hospital outpatient departments compared with physician offices. The association of these factors with the site of care varies across different gynecologic conditions.
Cognitive Conceptions of Teaching and Learning Motor Skills

It has recently been suggested that background knowledge, expectations about success and failure, motivation for learning, and the ability to understand instruction play a central role in determining how much a student learns. The purpose of this paper is to examine the literature for evidence that student thought processes mediate achievement in physical education. A cognitive conception of learning is presented, and the notion that students play an active role in the learning process is explored.