Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of surgical outcomes of intra-articular calcaneal fractures by age.

BACKGROUND Internal fixation is an accepted treatment for displaced fractures of the calcaneus. Operative intervention in older patients, however, is traditionally discouraged in the literature. The purpose of this study was to compare the outcomes of internal fixation of intra-articular fractures of the calcaneus on the basis of patient age. METHODS One hundred and seventy-five patients (191 fractures) who underwent internal fixation between 1992 and 2007 for a displaced, intra-articular calcaneal fracture were identified. The American Society of Anesthesiologists score, the fracture pattern, and the mechanism of injury were recorded. Each patient was contacted to complete a follow-up survey from which clinical outcome scores were calculated. One hundred and forty-six patients with 158 fractures were available for follow-up and were divided into two groups for comparison. Group I consisted of 108 fractures in patients who were less than fifty years old. Group II was composed of fractures in fifty patients who were fifty years of age or older. RESULTS The mean duration of follow-up was 8.98 years. The average patient age was thirty-six years for Group I and fifty-eight years for Group II. The average adjusted American Orthopaedic Foot & Ankle Society score was 64 for Group I and 75 for Group II. The mean calcaneal fracture scoring system score was 66 for Group I, and 76 for Group II. Similarly, the average Foot Function Index was 24 and 15 for Groups I and II, respectively. Each clinical outcome measure suggests significantly better outcomes for Group II as compared with Group I (all p < 0.05). Overall, the complication rates were similar between groups. Conversion to subtalar fusion was 15% for Group I and 8% for Group II. CONCLUSIONS In this series, outcomes of older patients are at least equivalent to those of younger patients undergoing internal fixation for an intra-articular calcaneal fracture. Operative intervention appears to be a reasonable option for displaced calcaneal fractures in older patients. Physiologic age should be considered when evaluating older patients, and individualized treatment plans remain critical because patients with low physical demands or who have medical complications may be better candidates for nonoperative treatment. Prospective studies are needed in this area.
Mur Regulates the Gene Encoding the Manganese Transporter MntH in Brucella abortus 2308

ABSTRACT MntH is the only high-affinity manganese transporter identified in Brucella. A previous study showed that MntH is required for the wild-type virulence of Brucella abortus 2308 in mice (Anderson ES, et al., Infect. Immun. 77:3466–3474, 2009) and indicated that the mntH gene is regulated in a manganese-responsive manner in this strain by a Mur homolog. In the study presented here, the transcriptional start site for mntH in B. abortus 2308 was determined by primer extension analysis. Specific interactions between Mur and the mntH promoter region were demonstrated in an electrophoretic mobility shift assay (EMSA), and a Mur binding site was identified in the −55 to −24 region of the mntH promoter by DNase I footprint analysis. The specificity of the interaction of Mur with the putative Mur box was further evaluated by EMSA employing oligonucleotides in which the consensus nucleotides in this region were substituted. These studies not only confirm a direct role for Mur in the Mn-responsive regulation of mntH expression in Brucella abortus 2308 but also identify the cis-acting elements upstream of mntH that are responsible for this regulation.
A newly identified mouse hypothalamic area having bidirectional neural connections with the lateral septum: the perifornical area of the anterior hypothalamus rich in chondroitin sulfate proteoglycans

While previous studies and brain atlases divide the hypothalamus into many nuclei and areas, uncharacterised regions remain. Here, we report a new region in the mouse anterior hypothalamus (AH), a triangular‐shaped perifornical area of the anterior hypothalamus (PeFAH) between the paraventricular hypothalamic nucleus and fornix, that abundantly expresses chondroitin sulfate proteoglycans (CSPGs). The PeFAH strongly stained with markers for chondroitin sulfate/CSPGs such as Wisteria floribunda agglutinin and antibodies against aggrecan and chondroitin 6 sulfate. Nissl‐stained sections of the PeFAH clearly distinguished it as a region of comparatively low density compared to neighboring regions, the paraventricular nucleus and central division of the anterior hypothalamic area. Immunohistochemical and DNA microarray analyses suggested that PeFAH contains sparsely distributed calretinin‐positive neurons and a compact cluster of enkephalinergic neurons. Neuronal tract tracing revealed that both enkephalin‐ and calretinin‐positive neurons project to the lateral septum (LS), while the PeFAH receives input from calbindin‐positive LS neurons. These results suggest bidirectional connections between the PeFAH and LS. Considering neuronal subtype and projection, part of PeFAH that includes a cluster of enkephalinergic neurons is similar to the rat perifornical nucleus and guinea pig magnocellular dorsal nucleus. Finally, we examined c‐Fos expression after several types of stimuli and found that PeFAH neuronal activity was increased by psychological but not homeostatic stressors. These findings suggest that the PeFAH is a source of enkephalin peptides in the LS and indicate that bidirectional neural connections between these regions may participate in controlling responses to psychological stressors.
Purification and characterization of active caspase‐14 from human epidermis and development of the cleavage site‐directed antibody

Restricted expression of caspase‐14 in differentiating keratinocytes suggests the involvement of caspase‐14 in terminal differentiation. We purified active caspase‐14 from human cornified cells with sequential chromatographic procedures. Specific activity increased 764‐fold with a yield of 9.1%. Purified caspase‐14 revealed the highest activity on WEHD‐methylcoumaryl‐amide (MCA), although YVAD‐MCA, another caspase‐1 substrate, was poorly hydrolyzed. The purified protein was a heterodimer with 17 and 11 kDa subunits. N‐terminal and C‐terminal analyses demonstrated that the large subunit consisted of Ser6‐Asp146 and N‐terminal of small subunit was identified as Lys153. We successfully developed an antiserum (anti‐h14D146) directed against the Asp146 cleavage site, which reacted only with active caspase‐14 but not with procaspase‐14. Furthermore we confirmed that anti‐h14D146 did not show any reactivity to the active forms of other caspases. Immunohistochemical analysis demonstrated that anti‐h14D146 staining was mostly restricted to the cornified layer and co‐localized with some of the TUNEL positive‐granular cells in the normal human epidermis. UV radiation study demonstrated that caspase‐3 was activated and co‐localized with TUNEL‐positive cells in the middle layer of human epidermis. In contrast, we could not detect caspase‐14 activation in response to UV. Our study revealed tightly regulated action of caspase‐14, in which only the terminal differentiation of keratinocytes controls its activation process. J. Cell. Biochem. 109: 487–497, 2010. © 2009 Wiley‐Liss, Inc.
Towards evidence based circumcision of English boys: survey of trends in practice

Although the proportion of English boys circumcised for medical reasons fell from 35% in the early 1930s to 6.5% by the mid-1980s, even latterly it was argued that some two thirds underwent the procedure unnecessarily,1 a judgment consistent with practice in Scandinavia, where less than 2% of boys are circumcised.2 Has any further change occurred in English practice, and, if so, is this evidence based? We examined trends in the catchment population of a children's hospital, in its surrounding region, and in England as a whole. The study was confined to medically indicated operative circumcisions. Statistics for circumcisions for the NHS, including diagnostic codings, were obtained for the Mersey region and its health districts for 1975-97 and for England for 1984-6 and 1990-8. Data for 1996-8 may slightly underestimate the number of procedures performed.3 Corresponding population figures were supplied by the Office …
Processing of rotavirus glycoprotein VP7: implications for the retention of the protein in the endoplasmic reticulum

Rotaviruses are icosahedral particles that assemble in the lumen of the endoplasmic reticulum (ER). The viral glycoprotein, VP7, is also directed into this compartment and is retained for assembly onto the surface of viral cores. VP7 is therefore a resident ER glycoprotein with a luminal orientation. The VP7 gene possesses two potential in- frame initiation codons, each preceding a hydrophobic domain. Mature VP7 is derived from a precursor by cleavage but the site of cleavage has not been determined because viral VP7 has a blocked amino terminus. Using site-directed mutagenesis of the gene and in vitro transcription and translation systems, we have investigated the synthesis and processing of the primary products synthesized from each initiation codon. Proteins translated from either codon were processed in vitro to yield products indistinguishable in size. The primary translation products therefore appeared to be cleaved at the same site. The site was located empirically between Ala50 and Gln51 and mutation of the gene to convert Ala50----Val prevented processing. Amino-terminal sequence analyses of proteins synthesized in vitro, and characterization of an amino-terminal fragment of VP7 purified from virus unequivocally established Gln51 as the amino-terminal residue. Pyroglutamic acid was tentatively identified as the blocking group. Processing of VP7 therefore removes both amino-terminal hydrophobic domains from the protein. Some other mechanism not requiring the presence of these hydrophobic sequences must account for the retention of this novel glycoprotein in the ER.
Fatigue life prediction and modal analysis of carbon fiber reinforced composites

Abstract Fiber reinforced composite materials are finding increasing applications in aerospace structures, naval industry, military and automotive industry, because of their high specific stiffness and strength. The fatigue of composite materials is not completely understood which is quite different from metal fatigue. The objective of research is to apply modal testing for the fatigue life prediction of Carbon Fiber Reinforced Plastic laminate in plane bending with different fiber orientations. CFRP laminates with three fiber orientations as 0°, 45° and 90° are applied to fatigue loading in plane bending with R = -1 and S-N f . curves are plotted and power equations are determined. For modal testing of the laminates the specimens previously undergone fatigue loading are tested at specific intervals to determine the effect of modal characteristics on fatigue life of these laminates. The modal characteristics are determined by using FFT analyzer and the frequency effect on fatigue life is determined. Results are plotted between the fatigue life ratio and the natural frequency and empirical equations are generated for predication of fatigue life prediction. From the results it is concluded that the modal testing is an effective nondestructive testing method that can be applied for estimating the fatigue life of composite laminates
Effects of magnesium sulphate on postoperative coagulation, measured by rotational thromboelastometry (ROTEM®)*

We investigated the effects of magnesium sulphate on blood coagulation profiles using rotational thromboelastometry in gynaecological patients undergoing pelviscopic surgery. Patients were randomly allocated to the magnesium group (n = 20) or control group (n = 20). The magnesium group received magnesium sulphate (50 mg.kg−1 followed by continuous infusion of 15 mg.kg−1.h−1), whereas the control group received the same volume of isotonic saline according to the same methods. Mean (SD) postoperative serum magnesium levels were 1.58 (0.17) mmol.l−1 in the magnesium group compared with 0.98 (0.06) mmol.l−1 in the control group (p < 0.001). Postoperative clotting time, clot formation time, α‐angle and maximum clot firmness of INTEM, and clot formation time, α‐angle, and maximum clot firmness of EXTEM were significantly different between the two groups (p < 0.05). Intra‐operative infusion of magnesium sulphate seems to attenuate postoperative hypercoagulability by maintaining magnesium levels at the upper limit of the normal range.
The E1–E2 center in gallium arsenide is the divacancy

Based on defect energy levels computed from first-principles calculations, it is shown the E1–E2 center in irradiated GaAs cannot be due to an isolated arsenic vacancy. The only simple intrinsic defect with levels compatible with E1 and E2 is the divacancy. The arsenic monovacancy is reassigned to the E3 center in irradiated GaAs. These new assignments are shown to reconcile a number of seemingly contradictory experimental observations.
Patient complaints about hospital services: applying a complaint taxonomy to analyse and respond to complaints.

OBJECTIVE To explore the applicability of a patient complaint taxonomy to data on serious complaint cases. DESIGN Qualitative descriptive study. SETTING Complaints made to the New South Wales (NSW) Health Care Complaints Commission, Australia between 2005 and 2010. PARTICIPANTS All 138 cases of serious complaints by patients about public hospitals and other health facilities investigated in the 5-year period. MAIN OUTCOME MEASURE A thematic analysis of the complaints was conducted to identify particular complaint issues and the Reader et al. (Patient complaints in healthcare systems: a systematic review and coding taxonomy. BMJ Qual Saf 2014;23:678-89.) patient complaint taxonomy was then used to classify these issues into categories and sub-categories. RESULTS The 138 investigated cases revealed 223 complaint issues. Complaint issues were distributed into the three domains of the patient complaint taxonomy: clinical, management and relationships. Complaint issue most commonly related to delayed diagnosis, misdiagnosis, medication errors, inadequate examinations, inadequate/nil treatment and quality of care including nursing care. CONCLUSIONS The types of complaints from patients about their healthcare investigated by the NSW Commission were similar to those received by other patient complaint entities in Australia and worldwide. The application of a standard taxonomy to large numbers of complaints cases from different sources would enable the creation of aggregated data. Such data would have better statistical capacity to identify common safety and quality healthcare problems and so point to important areas for improvement. Some conceptual challenges in devising and using a taxonomy must be addressed, such as inherent problems in ensuring coding consistency, and giving greater weight to patient concerns about their treatment.
Influences of the current density on the performances of the chrome-plated layer in deterministic electroplating repair

Deterministic electroplating repair is a novel method for rapidly repairing the attrited parts. By the qualitative contrast and quantitative comparison, influences of the current density on performances of the chrome-plated layer were concluded in this study. The chrome-plated layers were fabricated under different current densities when the other parameters were kept constant. Hardnesses, thicknesses and components, surface morphologies and roughnesses, and wearability of the chrome-plated layers were detected by the Vickers hardness tester, scanning electron microscope / energy dispersive X-ray detector, digital microscope in the 3D imaging mode, and the ball-milling instrument with profilograph, respectively. In order to scientifically evaluate each factor, the experimental data was normalized. A comprehensive evaluation model was founded to quantitative analyse influence of the current density based on analytic hierarchy process method and the weighted evaluation method. The calculated comprehensive evaluation indexes corresponding to current density of 40A/dm2, 45A/dm2, 50A/dm2, 55A/dm2, 60A/dm2, and 65A/dm2 were 0.2246, 0.4850, 0.4799, 0.4922, 0.8672, and 0.1381, respectively. Experimental results indicate that final optimal option was 60A/dm2, and the priority orders were 60A/dm2, 55A/dm2, 45A/dm2, 50A/dm2, 40A/dm2, and 65A/dm2.
Comprehensive Spectrum of the β-Thalassemia Mutations in Khuzestan, Southwest Iran

β-Thalassemia (β-thal) is characterized by reduction or absence of β-globin gene expression. We describe the spectrum of mutations observed in a large cohort of β-thal carriers in Khuzestan, Southwest Iran. All together 1,241 blood samples from individuals with decreased mean corpuscular volume (MCV) and elevated Hb A2 levels, were analyzed either by reverse dot-blot or by direct sequencing of the HBB gene. We found 42 different mutations associated with β-thal and identified eight common β-globin variants, namely, Hb S [β6(A3)Glu→Val], Hb C [β6(A3)Glu→Lys], Hb D-Punjab [β121(GH4)Glu→Gln] and Hb O-Arab [β121(GH4)Glu→Lys]. No mutations were found in two individuals. The distribution is characteristic of a heterogenous population with three preferential mutations being present [codons 36/37 (–T), IVS-II-1 (G>A) and IVS-I-110 (G>A)] at a frequency of 20.5, 20.0 and 14.2%, respectively, followed by 39 mutations in decreasing frequencies from 5.2 down to 0.1%. These data are of importance when planning prevention strategies in the country.
The Polyketide Synthase Gene pks4 of Trichoderma reesei Provides Pigmentation and Stress Resistance

ABSTRACT Species of the fungal genus Trichoderma (Hypocreales, Ascomycota) are well-known for their production of various secondary metabolites. Nonribosomal peptides and polyketides represent a major portion of these products. In a recent phylogenomic investigation of Trichoderma polyketide synthase (PKS)-encoding genes, the pks4 from T. reesei was shown to be an orthologue of pigment-forming PKSs involved in synthesis of aurofusarin and bikaverin in Fusarium spp. In this study, we show that deletion of this gene in T. reesei results in loss of green conidial pigmentation and in pigmentation alteration of teleomorph structures. It also has an impact on conidial cell wall stability and the antagonistic abilities of T. reesei against other fungi, including formation of inhibitory metabolites. In addition, deletion of pks4 significantly influences the expression of other PKS-encoding genes of T. reesei. To our knowledge, this is the first indication that a low-molecular-weight pigment-forming PKS is involved in defense, mechanical stability, and stress resistance in fungi.
Xenogeneic Bio‐Root Prompts the Constructive Process Characterized by Macrophage Phenotype Polarization in Rodents and Nonhuman Primates

Tissue or organ regeneration using xenogeneic matrices is a promising approach to address the shortage of donor matrices for allotransplantation. Success of such approach has been demonstrated to correlate with macrophage‐mediated fibrotic homeostasis and tissue remodeling. The previous studies have demonstrated that treated dentin matrix (TDM) could be a suitable bioactive substrate for allogeneic tooth root regeneration. This study constructed xenogeneic bioengineered tooth root (bio‐root) via a combination of porcine TDM (pTDM) with allogeneic dental follicle cells (DFCs). Macrophage phenotypes are used to evaluate the remodeling process of xenogeneic bio‐roots in vitro and in vivo. pTDM can facilitate odontoblast differentiation of human derived DFCs. Xenogeneic bio‐roots in rat subcutaneous tissue prompt constructive response via M1 macrophage infiltration during early postimplantation stages and increase restorative M2 phenotype at later stages. After implantation of bio‐roots into jaws of rhesus monkeys for six months, periodontal ligament‐like fibers accompanied by macrophage polarization are observed, which are positive for COL‐1, Periostin, βIII‐tubulin and display such structures as fibroblasts and blood vessels. The reconstructed bio‐root possesses biomechanical properties for the dissipation of masticatory forces. These results support that xenogeneic bio‐root could maintain fibrotic homeostasis during remodeling process and highlight the potential application of xenogeneic matrices in regenerative medicine.
A multivariable model using advanced cytologic methods for the evaluation of indeterminate pancreatobiliary strictures.

BACKGROUND & AIMS Ancillary cytologic tests including digital image analysis (DIA) and fluorescence in situ hybridization (FISH) have been developed to improve the sensitivity of routine cytology (RC) for the diagnosis of malignancy in pancreatobiliary strictures. The goal of this study was to retrospectively compare the performance of RC, DIA, and FISH on clinical brushing specimens. METHODS Endoscopic retrograde cholangiopancreatography brushings were obtained from 498 consecutive patients with pancreatobiliary strictures and analyzed by RC, DIA, and FISH as per standard practice. RC diagnostic categories included negative, atypical, suspicious, or positive. Aneuploid/tetraploid histograms were considered positive for DIA. FISH was performed using UroVysion (Abbott Molecular, Inc, Des Plaines, IL) and classified as negative, trisomy, tetrasomy, or polysomy. RESULTS The sensitivity of polysomy FISH (42.9%) was significantly higher than RC (20.1%) when equivocal RC results were considered negative (P < .001) with identical specificity (99.6%). There was a significant difference in time for diagnosis of carcinoma between FISH diagnostic categories (P < .001) and between RC diagnostic categories (P < .001). Logistic regression analysis revealed that polysomy FISH, trisomy FISH, suspicious cytology, primary sclerosing cholangitis status, and age were associated with carcinoma (P < .05). CONCLUSIONS Polysomy FISH had high sensitivity without compromise to specificity. DIA was not a significant independent predictor of malignancy. Multivariable modeling using RC, FISH, age, and primary sclerosing cholangitis status can be used to estimate the probability of carcinoma for an individual patient. We recommend including FISH as a routine test where available, along with RC, in the evaluation of indeterminate pancreatobiliary strictures.
Emergence of superconductivity in the canonical heavy-electron metal YbRh2Si2

Going to extremes to find superconductivity Quantum phase transitions (QPTs) occur at zero temperature when parameters such as magnetic field or pressure are varied. In heavy fermion compounds, superconductivity often accompanies QPTs, a seeming exception being the material YbRh2Si2, which undergoes a magnetic QPT. Schuberth et al. performed magnetic and calorimetric measurements at extremely low temperatures and magnetic fields and found that it does become superconducting after all. Almost simultaneously with superconductivity, another order appeared that showed signatures of nuclear spin origin. Science, this issue p. 485 Magnetic and calorimetric measurements at extremely low temperatures and magnetic fields find superconductivity in YbRh2Si2. The smooth disappearance of antiferromagnetic order in strongly correlated metals commonly furnishes the development of unconventional superconductivity. The canonical heavy-electron compound YbRh2Si2 seems to represent an apparent exception from this quantum critical paradigm in that it is not a superconductor at temperature T ≥ 10 millikelvin (mK). Here we report magnetic and calorimetric measurements on YbRh2Si2, down to temperatures as low as T ≈ 1 mK. The data reveal the development of nuclear antiferromagnetic order slightly above 2 mK and of heavy-electron superconductivity almost concomitantly with this order. Our results demonstrate that superconductivity in the vicinity of quantum criticality is a general phenomenon.