Miracle-dz/UniFAR-FTUs huggingface dataset:
Polymorphisms of the TNF-α gene interact with plasma fatty acids on inflammatory biomarker profile: a population-based, cross-sectional study in São Paulo, Brazil

Abstract The aim of the present study was to investigate the relationship of four TNF-α SNP with inflammatory biomarkers and plasma fatty acids (FA), and the interaction among them in a population-based, cross-sectional study in São Paulo, Brazil. A total of 281 subjects, aged >19 and <60 years, participated in a cross-sectional, population-based study performed in Brazil. The following SNP spanning the TNF-α gene were genotyped: -238G/A (rs361525), -308G/A (rs1800629), -857C/T (rs1799724) and -1031T/C (rs1799964). In all, eleven plasma inflammatory biomarkers and plasma FA profile were determined. To analyse the interaction between TNF-α SNP and plasma FA, a cluster analysis was performed to stratify individuals based on eleven inflammatory biomarkers into two groups used as outcome: inflammatory (INF) and non-inflammatory clusters. The -238A allele carriers had higher TNF-α (P=0·033), IL-6 (P=0·013), IL-1β (P=0·037), IL-12 (0·048) and IL-10 (P=0·010) than the GG genotype. The -308A allele carriers also had lower levels of plasma palmitoleic acid (P=0·009), oleic acid (P=0·039), total MUFA (P=0·014), stearoyl-CoA desaturase (SCD) activity index-16 (P=0·007), SCD-18 (P=0·020) and higher levels of PUFA (P=0·046) and DHA (P=0·044). Significant interactions modifying the risk of belonging to the INF cluster were observed with inflammatory cluster as outcome between -857C/T and plasma α-linolenic acid (P=0·026), and also between -308G/A and plasma stearic acid (P=0·044) and total SFA (P=0·040). Our study contributes to knowledge on TNF-α SNP and their association with inflammatory biomarker levels, plasma FA and the interaction among them, of particular interest for the Brazilian population.
Epidemiologic aspects and laboratory features of enterovirus infections in Western Germany, 2000–2005

From 2000 to 2005, a total of 1,096 enterovirus infections were diagnosed either by isolation of virus from cell culture or by RT‐PCR (5′non‐coding region (NCR)). Typing of viruses (n = 674) was carried out by immunofluorescence with monoclonal antibodies, neutralization test or molecular methods. Seasons with high enterovirus activity were characterized by high prevalence of echovirus 30 (62.2% in 2000, 25.5% in 2001) and echovirus 13 (34.5% in 2001). In contrast, in the 2003 season, which had very low enterovirus activity, these types were rare. During this season, cell culture sensitivity (human colonic carcinoma cells and human embryonic lung fibroblasts (HEL)) was exceptionally low. In order to determine the type of “non‐cultivable” enteroviruses, purified RNA from selected stool samples was subjected to direct molecular typing. VP1/2A‐specific fragments were amplified by RT‐PCR, cloned and sequenced. The predominant virus identified was coxsackie A. Consequently, rhabdomyosarcom cells were introduced into the daily routine, which improved the isolation of enteroviruses. Echovirus 30 was again most commonly isolated during seasons 2004 and 2005 with increasing enterovirus activity. In conclusion, high prevalence of echovirus 30 and 13 is indicative of seasons with high enterovirus activity. The type of circulating enteroviruses may influence isolation of enterovirus from cell culture. RT‐PCR (VP1/2A) combined with cloning and sequencing of amplicons is a useful tool for viral typing directly from stool samples. In cases of severe enterovirus infection, virological diagnosis should not solely rely on virus isolation from cell culture. J. Med. Virol. 79:956‐962, 2007. © 2007 Wiley‐Liss, Inc.
Developmental Cognitive Neuroscience Development of the Default Mode and Central Executive Networks across Early Adolescence: a Longitudinal Study Keywords: Adolescent Brain Development Functional Connectivity Default Mode Network Central Executive Network Intelligence Early Adolescence

The mature brain is organized into distinct neural networks defined by regions demonstrating correlated activity during task performance as well as rest. While research has begun to examine differences in these networks between children and adults, little is known about developmental changes during early adolescence. Using functional magnetic resonance imaging (fMRI), we examined the Default Mode Network (DMN) and the Central Executive Network (CEN) at ages 10 and 13 in a longitudinal sample of 45 participants. In the DMN, participants showed increasing integration (i.e., stronger within-network correlations) between the posterior cingulate cortex (PCC) and the medial prefrontal cortex. During this time frame participants also showed increased segregation (i.e., weaker between-network correlations) between the PCC and the CEN. Similarly, from age 10 to 13, participants showed increased connectivity between the dorsolateral prefrontal cortex and other CEN nodes, as well as increasing DMN segregation. IQ was significantly positively related to CEN integration at age 10, and between-network segregation at both ages. These findings highlight early adolescence as a period of significant maturation for the brain's functional architecture and demonstrate the utility of longitudinal designs to investigate neural network development.
The need for school‐constructed theories in practice in US school restructuring

States that research on school restructuring provides little evidence that teacher‐student‐principal relationships are changing. Staffs may be confusing structural thinking (adopting and implementing organization structures such as shared governance, co‐operative learning groups, teacher‐student advisories) with normative thinking (reconceptualizing how norms characterizing ideal relationships among teachers, principals, and students can be supported and developed through organization structures). Argues that normative thinking requires staffs to reflect critically about their schools as workplaces but that US schools have not been reflective workplaces. As a vehicle for this normative thinking, suggests school‐site constructed theories in practice, which have two steps: critical inquiry and monitoring the change progress. Makes three policy suggestions based on the need for staffs to theorize about their practice: an action research role for professors; a caveat about prescribing only measurable outcomes for administrator preparation programmes; and time for teacher‐leader and principal reflection on their practice.
Characterization and purification of H1TF2, a novel CCAAT-binding protein that interacts with a histone H1 subtype-specific consensus element

Definition of mechanisms regulating human histone H1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. Two distinct binding activities have been identified in nuclear extracts from HeLa cells and mapped within a 180-base-pair (bp) region of a cell cycle-regulated H1 gene promoter. H1TF1 bound to an H1-specific A + C-rich sequence (AC box), 100 bp upstream of the cap site; H1TF2 interacted with the H1 subtype-specific consensus element and was dependent on the presence of an intact CCAAT box for binding. H1TF2 was purified through a combination of ion-exchange and oligonucleotide affinity chromatographies. Analysis of purified fractions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and UV crosslinking showed that H1TF2 was a single polypeptide of 47 kilodaltons. This factor was distinct from previously characterized CCAAT-binding proteins in both molecular size and binding properties. Fractions containing H1TF2 activity activated transcription in vitro only if programmed with an H1 DNA template carrying an intact H1TF2-binding site.
Cutaneous ulcers: An unusual manifestation of inherited thrombophilia

We report two cases with cutaneous lesions found to be associated with factor V Leiden mutation and low S protein levels. At the time of presentation, no other symptoms were reported. Histopathology of both lesions revealed the presence of widespread intravascular thrombi. The therapeutic response to oral anticoagulants in the first case was dramatic, leading to complete healing of the lesions. Inherited causes of thrombophilia manifested as cutaneous lesions, although rare, should be considered in the differential diagnosis of occlusive vasculopathy. Am J Hematol 76:139–142, 2004. © 2004 Wiley‐Liss, Inc.
An interaction-focused intervention approach to training everyday communication partners: a single case study

Background: Communication partner training (CPT) appears to be a growing area within aphasiology. Much of the work carried out so far has focused on training volunteers to have conversations with people with aphasia in order to improve communication and the person with aphasia’s (PWA’s) psychosocial well-being and/or on improving the ability of significant others to communicate information with the PWA within clinical tasks. In this paper, we present the results of a single-case intervention study that used an interaction-focused approach to target the conversational behaviours of the significant other of a PWA with the aim of improving the dyad’s everyday conversations within the home environment. Aims: To discuss the targeting, implementation, and evaluation of an interaction-focused intervention programme for a significant other of a PWA. Methods & Procedures: Conversation Analysis was used both to guide choice of individualised target behaviours for the intervention and to explore changes in the conversational interaction between a woman with mild-moderate aphasia and her life partner. Three samples of video-recorded natural conversational interaction from before and after the partner took part in a six-session long group intervention were analysed. The evidence for change that emerged from qualitative analysis of the conversational data was further analysed by an independent and blinded assessor doing quantitative comparisons. Outcomes & Results: There was qualitative and quantitative evidence that two of three targeted conversational behaviours had changed following the intervention programme. Following the completion of the intervention, the dyad spent significantly less time in pedagogic activities. Furthermore, the significant other showed an increased attention towards PWA’s conversational contributions. The combination of qualitative and quantitative analyses also revealed that postintervention, the communication partner displayed changes in an interactional behaviour that was not targeted in the intervention, that is, dismissive language towards the PWA. Conclusions: This study adds to the existing literature in presenting positive results from an intervention that used an interaction-focused approach, here targeted towards the everyday communication partner of a PWA. Notably, this study supplemented qualitative outcomes with blinded and statistical quantitative analyses. Also, the fact that no transcriptions were used during the intervention process and that therapy was delivered via group intervention shows the feasibility of this form of CPT in clinical settings. Furthermore, the study suggests that intervention programmes targeting the behaviours of a communication partner may produce positive change in conversational behaviours that have not been directly targeted in the intervention.
Caffeine Reduces Reaction Time and Improves Performance in Simulated-Contest of Taekwondo

The aim of this study was to investigate the effects of caffeine on reaction time during a specific taekwondo task and athletic performance during a simulated taekwondo contest. Ten taekwondo athletes ingested either 5 mg·kg−1 body mass caffeine or placebo and performed two combats (spaced apart by 20 min). The reaction-time test (five kicks “Bandal Tchagui”) was performed immediately prior to the first combat and immediately after the first and second combats. Caffeine improved reaction time (from 0.42 ± 0.05 to 0.37 ± 0.07 s) only prior to the first combat (P = 0.004). During the first combat, break times during the first two rounds were shorter in caffeine ingestion, followed by higher plasma lactate concentrations compared with placebo (P = 0.029 and 0.014, respectively). During the second combat, skipping-time was reduced, and relative attack times and attack/skipping ratio was increased following ingestion of caffeine during the first two rounds (all P < 0.05). Caffeine resulted in no change in combat intensity parameters between the first and second combat (all P > 0.05), but combat intensity was decreased following placebo (all P < 0.05). In conclusion, caffeine reduced reaction time in non-fatigued conditions and delayed fatigue during successive taekwondo combats.
Fast realistic multi-action recognition using mined dense spatio-temporal features

Within the field of action recognition, features and descriptors are often engineered to be sparse and invariant to transformation. While sparsity makes the problem tractable, it is not necessarily optimal in terms of class separability and classification. This paper proposes a novel approach that uses very dense corner features that are spatially and temporally grouped in a hierarchical process to produce an overcomplete compound feature set. Frequently reoccurring patterns of features are then found through data mining, designed for use with large data sets. The novel use of the hierarchical classifier allows real time operation while the approach is demonstrated to handle camera motion, scale, human appearance variations, occlusions and background clutter. The performance of classification, outperforms other state-of-the-art action recognition algorithms on the three datasets; KTH, multi-KTH, and Hollywood. Multiple action localisation is performed, though no groundtruth localisation data is required, using only weak supervision of class labels for each training sequence. The Hollywood dataset contain complex realistic actions from movies, the approach outperforms the published accuracy on this dataset and also achieves real time performance.
SPATIAL GENETIC STRUCTURE OF CLONAL AND SEXUAL REPRODUCTION IN POPULATIONS OF ADENOPHORA GRANDIFLORA (CAMPANULACEAE)

The spatial distribution of clonal versus sexual reproduction in plant populations should generally have differing effects on the levels of biparental inbreeding and the apparent selfing rate, produced via mating by proximity through limited pollen dispersal. We used allozyme loci, join‐count statistics, and Moran's spatial autocorrelation statistics to separate the spatial genetic structure caused by clonal reproduction from that maintained in sexually reproduced individuals in two populations of Adenophora grandiflora, a perennial herb. Join‐count statistics showed that there were statistically significant clustering of clonal genotypes within distances less than 4 m. Both the entire populations and the sets of sexually reproduced individuals exhibited significant spatial autocorrelation at less than about 12 m, and the sexually reproduced individuals are substantially structured in an isolation‐by‐distance manner, consistent with a neighborhood size of about 50.
Female Zebra Finches (Taeniopygia guttata) Are Chronically but Not Cumulatively “Anemic” during Repeated Egg Laying in Response to Experimental Nest Predation

Recently it has been recognized that reproduction itself, or the regulatory processes controlling reproduction, might contribute to physiological costs of reproduction. Reproductive anemia, a decrease in hematocrit and hemoglobin concentration, might provide one such mechanism underlying the costs of egg production in birds. In this study, we investigated the effect of repeated cycles of egg production in response to experimental nest predation (egg removal) on hematological traits in female zebra finches (Taeniopygia guttata). We predicted that if the negative effect of egg production on hematocrit and hemoglobin concentration was cumulative, with anemia being proportional to reproductive effort, then females laying more clutches, or laying successive clutches without recovery during incubation, would show greater reproductive anemia. In contrast, if females maintain hematocrit and hemoglobin concentration at some minimum functional level independent of reproductive effort, then there should be no difference in hematological traits among females laying two or more successive clutches. Our results supported the second of these hypotheses: egg‐laying females had reduced hematocrit (−7.5%) and hemoglobin concentrations (−10%), but the extent of reproductive anemia did not differ among females laying either two or three successive clutches, with or without recovery during incubation, or in females laying 7–21 eggs. Females maintained low hematocrit and hemoglobin for 20–35 d, and we suggest that prolonged periods of anemia might be common and functionally important in free‐living birds, for example, where females produce multiple successive clutches in response to high levels of nest predation or where they initiate a second clutch while still rearing first brood chicks.
Test Review: School Social Behavior Scales, Second Edition

The School Social Behavior Scales, Second Edition (SSBS-2; Merrell, 2002), published by Assessment-Intervention Resources, is one component of the Social Behavior Scales (SBS), a norm-referenced, standardized cross-informant system of behavior rating scales used to assess social competence and antisocial behavior of individuals aged 5 to 18 years (K-12th grades) across school, home, and community settings. The SSBS-2 is composed of two major scales, namely, the Social Competence scale (Scale A) and the Antisocial Behavior scale (Scale B), and is typically completed by educators such as teachers and administrators in school settings. Similar to the original School Social Behavior Scales (SSBS; Merrell, 1993) the SSBS-2 was revised to be consistent with the Home and Community Social Behavior Scales (HCSBS; Merrell & Caldarella, 2002), which is the second component of the SBS typically completed by parents, group home supervisors, and other community-based raters. The SSBS-2 was developed as a quick and easy way for teachers and other school personnel to identify individuals who are behaviorally at risk (i.e., individuals who exhibit significant social skills deficits and antisocial behavior problems); to assist in the development of appropriate interventions; to monitor progress during, and evaluate the effectiveness of, interventions; and to aid in understanding the social-behavioral characteristics and patterns of children and youths (Merrell, 2002). The two scales of the SSBS-2 (Social Competence and Antisocial Behavior) are printed separately on the two inside pages of the record form. Each scale consists of 32 items that are rated on a 5-point Likert-type scale, with higher Social Competence scores indicating greater levels of social adjustment and higher Antisocial Behavior scores indicating greater levels of antisocial behavior. The Social Competence and Antisocial Behavior scales are each composed of three subscales. The Social Competence scale is composed of the Peer Relations (14 items), Self-Management/Compliance (10 items), and Academic Behavior (8 items) subscales. The Antisocial Behavior scale is composed of the Hostile/Irritable (14 items), Antisocial/Aggressive (10 items), and Defiant/Disruptive (8 items) subscales. The items on the SSBS-2 are the same as those on the SSBS, with the exception of minor changes in wording and the deletion of one item from the Social Competence scale (Merrell, 2002).
The 2017 Hands in the Million Challenge on 3D Hand Pose Estimation

We present the 2017 Hands in the Million Challenge, a public competition designed for the evaluation of the task of 3D hand pose estimation. The goal of this challenge is to assess how far is the state of the art in terms of solving the problem of 3D hand pose estimation as well as detect major failure and strength modes of both systems and evaluation metrics that can help to identify future research directions. The challenge follows up the recent publication of BigHand2.2M and First-Person Hand Action datasets, which have been designed to exhaustively cover multiple hand, viewpoint, hand articulation, and occlusion. The challenge consists of a standardized dataset, an evaluation protocol for two different tasks, and a public competition. In this document we describe the different aspects of the challenge and, jointly with the results of the participants, it will be presented at the 3rd International Workshop on Observing and Understanding Hands in Action, HANDS 2017, with ICCV 2017.
Teachers on intercultural education

Opinions differ on the desirability of paying attention to intercultural education in schools and on the opportunities to do this. Theoretical agreement and conceptual clarity on the concept are hard to find. The theoretical debate on intercultural education is far removed from daily practice in the classroom. Ideally, experience‐related ideas and the insights of teachers should form part of the debate. In this article we present an example of measuring teachers' opinions on the concept of intercultural education. The research was carried out in The Netherlands with the help of a written questionnaire. Seventy‐four teachers participated. The researchers differentiated four accents in intercultural education, based on the literature. This differentiation was not reflected in the teachers' answers. They had a slight preference for a general pluralistic interpretation of intercultural education and, hence, for a particular pedagogical approach. We will explore this finding further in the discussion.
Manifold design for uniform fluid distribution in parallel microchannels

In this paper, we use an electrical circuit analogy to design a microfluidic manifold achieving a uniform distribution of flow across multiple channels with minimal pressure gradient throughout the flow. We investigate the impact of manifold design to the mass flow distribution derived from the electrical circuit analogy and then analyzed the flow distribution in microchannels using computational fluid dynamics including thermal modelling. We compared the flow uniformity in the parallel channels with other manifold designs and found that step manifold design has improved the flow and temperature uniformity of the parallel channels.
Dopamine acutely decreases apical membrane Na/H exchanger NHE3 protein in mouse renal proximal tubule.

BACKGROUND Dopamine is a principal natriuretic hormone in mammalian Na+ homeostasis. Dopamine acutely alters glomerular filtration rate (GFR) and decreases Na+ absorption in both the proximal and distal nephron. Proximal tubule natriuresis is effected through inhibition of the apical membrane Na/H exchanger NHE3. METHODS We examined whether dopamine directly and acutely decreases apical membrane NHE3 protein using renal tissue in two in vitro systems: renal cortical slices and in vitro perfused single tubules. After incubation with dopamine, NHE3 activity was measured by 22Na flux and NHE3 antigen was measured by immunoblot in apical membrane and total cellular membranes. RESULTS Direct application of dopamine to either cortical slices or microperfused tubules acutely decreases NHE3 activity and antigen at the apical membrane of the proximal tubule. No change in total cellular NHE3 was detected. CONCLUSION One mechanism by which dopamine causes natriuresis is via direct and acute reduction of NHE3 protein at the apical membrane via changes in NHE3 protein trafficking.