Miracle-dz/UniFAR-FTUs huggingface dataset:
Depressed responses of facilitatory synapses.

We show that when temporal summation takes place, depression of postsynaptic responses may ensue when the underlying synaptic conductance change is constant or even facilitatory. We term this phenomenon "apparent depression. " Such apparent depression is most notable for slow synaptic conductance changes, for high frequency, and when the synapse is located at distal dendritic sites. We show that, when temporal summation ensues, the erroneous estimation of short-term synaptic plasticity arises partially from the conventional measurement of synaptic dynamics at postsynaptic potential peak time. This can be corrected when measuring overlapping synaptic responses at fixed intervals after stimulus time. Somatic voltage clamp also helps to partially correct for the apparent depression, but a good model of the neuron can do even better in providing a more accurate view of the underlying synaptic conductances.
Stability and structural properties of stochastic periodic systems: An operator-spectral approach

This paper addresses the stability and structural properties of discrete-time linear stochastic periodic systems with multiplicative noise. First, according to the spectral placement of a monodromy operator, spectral criteria are presented for asymptotic mean square stability, weak stability, observability and detectability, respectively. Further, it is shown that under the condition of observability (detectability), the asymptotic mean square stability of concerned systems is equivalent to the existence of positive (semi-)definite solution of a generalized periodic Lyapunov equation.
OF THE EXPERIMENTAL ANALYSIS OF BEHAVIOR DISCRIMINATION LEARNING , THE PEAK SHIFT , AND BEHAVIORAL CONTRAST '

A discrimination between two successively alternating stimuli was trained under conditions that maintained equal frequencies of reinforcement in the presence of each of the discriminative stimuli (S1 and S2) but that also reduced the rate of responding to S2. These conditions included a multiple variable-interval differential-reinforcement-of-low-rate schedule and a multiple variable-interval variable-interval schedule in which responses to S2 were punished. Whenever the rate of responding to S2 was reduced, rate of responding to SI (behavioral contrast) increased, and the peak of a subsequently obtained generalization gradient did not occur at the expected location (between S1 and S2) but was displaced away from S2, below S1. Discrimination training in which the frequencies of reinforcement earned in S1 and S2 were not equal (variable-interval 1-min variable-interval 5-min training) produced contrast and the peak shift only if the rate of responding to S2 had been reduced, as after non-differential reinforcement in which variable-interval 1-min schedules were correlated with S1 and with S2. It was concluded that a sufficient condition for the occurrence of behavioral contrast and the peak shift was reduction of the rate of responding to one of two alternating discriminative stimuli and that a peak shift will occur only if contrast had occurred during discrimination training.
Graphite Electrode as a Sole Electron Donor for Reductive Dechlorination of Tetrachlorethene by Geobacter lovleyi

ABSTRACT The possibility that graphite electrodes can serve as the direct electron donor for microbially catalyzed reductive dechlorination was investigated with Geobacter lovleyi. In an initial evaluation of whether G. lovleyi could interact electronically with graphite electrodes, cells were provided with acetate as the electron donor and an electrode as the sole electron acceptor. Current was produced at levels that were ca. 10-fold lower than those previously reported for Geobacter sulfurreducens under similar conditions, and G. lovleyi anode biofilms were correspondingly thinner. When an electrode poised at −300 mV (versus a standard hydrogen electrode) was provided as the electron donor, G. lovleyi effectively reduced fumarate to succinate. The stoichiometry of electrons consumed to succinate produced was 2:1, the ratio expected if the electrode served as the sole electron donor for fumarate reduction. G. lovleyi effectively reduced tetrachloroethene (PCE) to cis-dichloroethene with a poised electrode as the sole electron donor at rates comparable to those obtained when acetate serves as the electron donor. Cells were less abundant on the electrodes when the electrodes served as an electron donor than when they served as an electron acceptor. PCE was not reduced in controls without cells or when the current supply to cells was interrupted. These results demonstrate that G. lovleyi can use a poised electrode as a direct electron donor for reductive dechlorination of PCE. The ability to colocalize dechlorinating microorganisms with electrodes has several potential advantages for bioremediation of subsurface chlorinated contaminants, especially in source zones where electron donor delivery is challenging and often limits dechlorination.
Overexpression of heat shock protein 72 in transgenic mice decreases infarct size in vivo.

BACKGROUND Previous studies have demonstrated that induction of heat shock protein (HSP) 72 by whole-body hyperthermia reduces infarct size in an in vivo model of ischemia and reperfusion. Furthermore, hearts obtained from transgenic mice that overexpress HSP72 demonstrate improved functional recovery and decreased infarct size in vitro after global ischemia and reperfusion. METHODS AND RESULTS To test the hypothesis that overexpression of HSP72 in transgenic mice reduces infarct size in vivo, transgenic mice that were heterozygous for a rat HSP70i gene ([+]HSP72) and transgene-negative littermate controls ([-]HSP72) were subjected to 30 minutes of left coronary artery occlusion followed by 120 minutes of reperfusion. Core body temperature was monitored with a rectal thermometer and maintained between 36.5 degrees C and 37.0 degrees C with a heating pad. Infarct size, determined by dual staining with triphenyltetrazolium chloride and phthalocyanine blue dye, was smaller in [+]HSP72 mice compared with [-]HSP72 mice (12.7 +/- 2.8% [n = 7] versus 33.4 +/- 4.5% [n = 6], infarct size/risk area, respectively; P < .05; mean +/- SEM). CONCLUSIONS Overexpression of HSP72 reduces infarct size in this in vivo transgenic mouse model of myocardial ischemia and reperfusion.
Identification of ORC1/CDC6-Interacting Factors in Trypanosoma brucei Reveals Critical Features of Origin Recognition Complex Architecture

DNA Replication initiates by formation of a pre-replication complex on sequences termed origins. In eukaryotes, the pre-replication complex is composed of the Origin Recognition Complex (ORC), Cdc6 and the MCM replicative helicase in conjunction with Cdt1. Eukaryotic ORC is considered to be composed of six subunits, named Orc1–6, and monomeric Cdc6 is closely related in sequence to Orc1. However, ORC has been little explored in protists, and only a single ORC protein, related to both Orc1 and Cdc6, has been shown to act in DNA replication in Trypanosoma brucei. Here we identify three highly diverged putative T. brucei ORC components that interact with ORC1/CDC6 and contribute to cell division. Two of these factors are so diverged that we cannot determine if they are eukaryotic ORC subunit orthologues, or are parasite-specific replication factors. The other we show to be a highly diverged Orc4 orthologue, demonstrating that this is one of the most widely conserved ORC subunits in protists and revealing it to be a key element of eukaryotic ORC architecture. Additionally, we have examined interactions amongst the T. brucei MCM subunits and show that this has the conventional eukaryotic heterohexameric structure, suggesting that divergence in the T. brucei replication machinery is limited to the earliest steps in origin licensing.
A Simple Risk Stratification for Time to Development of Sight-Threatening Diabetic Retinopathy

OBJECTIVE The American Diabetes Association and the English NHS Diabetic Eye Screening Program recommend annual screening for diabetic retinopathy (DR) with referral to ophthalmology clinics of patients with sight-threatening DR (STDR). Using only longitudinal data from retinal photographs in the population-based NHS Diabetic Eye Screening Program in Gloucestershire, we developed a simple means to estimate risk of STDR. RESEARCH DESIGN AND METHODS From 2005, 14,554 patients with no DR or mild nonproliferative DR only at two consecutive annual digital photographic screenings were categorized by the presence of DR in neither, one, or both eyes at each screening and were followed for a further median 2.8 years. RESULTS Of 7,246 with no DR at either screening, 120 progressed to STDR, equivalent to an annual rate of 0.7%. Of 1,778 with no DR in either eye at first screening and in one eye at second screening, 80 progressed to STDR, equivalent to an annual rate of 1.9% and to a hazard ratio (HR) of 2.9 (95% CI 2.2–3.8) compared with those with no DR. Of 1,159 with background DR in both eyes at both screenings, 299 progressed to STDR equivalent to an annual rate of 11% and an HR of 18.2 (14.7–22.5) compared with individuals with no DR. CONCLUSIONS Combining the results from 2 consecutive years of photographic screening enables estimation of the risk of future development of STDR. In countries with systematic screening programs, these results could inform decisions about screening frequency.
In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument

Abstract. We used a probe-based dual-modality optical imaging instrument to measure in vivo coating thickness distributions of a gel distributed along the vaginal lumen, in a clinical study. The gel was a surrogate for one delivering an anti-HIV topical microbicide. Imaging data from Fourier-domain multiplexed low-coherence interferometry (mLCI) and fluorimetric measurements were compared to assess the feasibility and accuracy of mLCI in measuring in vivo gel coating thickness distributions. In each study session, 3.5 mL of Replens gel was inserted to the vaginal fornix while the participant was supine. The participant either: 1. remained supine (10 or 60 min); or 2. sat up (1 min), stood up (1 min), sat down (1 min) and returned to the supine position; net elapsed time was 10 or 60 min after which the gel distribution was imaged. Local coating thickness distributions were qualitatively and quantitatively similar. Here mLCI did not accurately measure thicker gel coatings (>0.8 mm), a limitation not seen with fluorimetry. However, mLCI is capable of measuring in vivo microbicide gel distributions with resolution on the order of 10 μm, without the need for exogenous contrast agents, and can accurately capture relevant summary coating measures in good agreement with fluorimetry.
Theoretical Explanations for Firms' Information Privacy Behaviors

Information privacy is an important information management issue that is increasingly challenging managers and policy makers. While many studies have investigated information privacy as an individual, sectoral, or national level phenomenon, there is a gap in our understanding of organizational approaches to developing and implementing policies and programs to manage customer information privacy. Information systems research lacks theory to explain firm level information privacy behaviors. This article argues for an expanded repertoire of theories to be applied to investigating information privacy, especially the role that the pursuit of competitive necessity versus competitive advantage plays in explaining organizational level behavior. The authors outline how the Institutional Approach (IA) and the Resource-Based View (RBV) of the firm offer compelling theoretical explanations for firms’ behaviors and should be applied to privacy research within the information systems area.
A systematic review of the physical activity assessment tools used in primary care

Background Primary care is an ideal setting for physical activity interventions to prevent and manage common long-term conditions. To identify those who can benefit from such interventions and to deliver tailored support, primary care professionals (e.g. GPs, practice nurses, physiotherapists, health care assistants) need reliable and valid tools to assess physical activity. However, there is uncertainty about the best-performing tool. Objective To identify the tools used in the literature to assess the physical activity in primary care and describe their psychometric properties. Method A systematic review of published and unpublished literature was undertaken up to 1 December 2016). Papers detailing physical activity measures, tools or approaches used in primary care consultations were included. A synthesis of the frequency and context of their use, and their psychometric properties, was undertaken. Studies were appraised using the Downs and Black critical appraisal tool and the COnsensus-based Standards for the selection of health Measurement Instruments (COSMIN) initiative checklist. Results Fourteen papers reported 10 physical activity assessment tools. The General Practice Physical Activity Questionnaire (GPPAQ) was most frequently reported. None of the assessment tools identified showed high reliability and validity. Intra-rater reliability ranged from kappa: 0.53 [Brief Physical Activity Assessment Tool (BPAAT)] to 0.67 (GPPAQ). Criterion validity ranged from Pearson's rho: 0.26 (GPPAQ) to 0.52 (Physical Activity Vital Sign). Concurrent validity ranged from kappa: 0.24 (GPPAQ) to 0.64 (BPAAT). Conclusion The evidence base about physical activity assessment in primary care is insufficient to inform current practice.
MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src

Membrane-type-1 matrix metalloproteinase (MT1-MMP) is a zinc-dependent type-I transmembrane metalloproteinase involved in pericellular proteolysis, migration and invasion, with elevated levels correlating with a poor prognosis in cancer. MT1-MMP-mediated transcriptional regulation of genes in cancer cells can contribute to tumour growth, although this is poorly understood at a mechanistic level. In this study, we investigated the mechanism by which MT1-MMP regulates the expression of VEGF-A in breast cancer cells. We discovered that MT1-MMP regulates VEGFR-2 cell surface localisation and forms a complex with VEGFR-2 and Src that is dependent on the MT1-MMP hemopexin domain and independent of its catalytic activity. Although the localisation of VEGFR-2 was independent of the catalytic and intracellular domain of MT1-MMP, intracellular signalling dependent on VEGFR-2 activity leading to VEGF-A transcription still required the MT1-MMP catalytic and intracellular domain, including residues Y573, C574 and DKV582. However, there was redundancy in the function of the catalytic activity of MT1-MMP, as this could be substituted with MMP-2 or MMP-7 in cells expressing inactive MT1-MMP. The signalling cascade dependent on the MT1-MMP–VEGFR-2–Src complex activated Akt and mTOR, ultimately leading to increased VEGF-A transcription.
A High-Speed Inkjet-Printed Microelectromechanical Relay With a Mechanically Enhanced Double-Clamped Channel-Beam

We report a high-speed inkjet-printed three-terminal microelectromechanical (MEM) relay with a double-clamped beam that exploits the enhanced stiffness of the double-clamped structure to improve electrical performance. To minimize mechanical delay and pull-in voltage, the contact gap between the channel-beam and drain, and the stiffness of the beam and shape of the drain was carefully designed and optimized through a 3-D finite element simulation. The double-clamped beam prevents stiction-related failure between the channel-beam and drain despite the contact gap being only 370 nm for a <inline-formula> <tex-math notation="LaTeX">$> 500~\mu \text{m}$ </tex-math></inline-formula> long beam. The resulting printed relay delivers a turn-ON delay of <inline-formula> <tex-math notation="LaTeX">$8~\mu \text{s}$ </tex-math></inline-formula> at a gate voltage of 10 V, a pull-in voltage of only 7.2 V, immeasurable off-leakage, excellent subthreshold swing, and a small hysteresis window of 2 V without any bending or collapsing of the beam. The device also shows reliable operation over <inline-formula> <tex-math notation="LaTeX">$10^{5}$ </tex-math></inline-formula> cycles while maintaining a high ON/OFF ratio of <inline-formula> <tex-math notation="LaTeX">$10^{8}$ </tex-math></inline-formula>, and extremely low ON-state resistance of 3.7 <inline-formula> <tex-math notation="LaTeX">$\Omega $ </tex-math></inline-formula>. [2016-0180]
TRACKS: toward directable thin shells

We combine the often opposing forces of artistic freedom and mathematical determinism to enrich a given animation or simulation of a surface with physically based detail. We present a process called tracking, which takes as input a rough animation or simulation and enhances it with physically simulated detail. Building on the foundation of constrained Lagrangian mechanics, we propose weak-form constraints for tracking the input motion. This method allows the artist to choose where to add details such as characteristic wrinkles and folds of various thin shell materials and dynamical effects of physical forces. We demonstrate multiple applications ranging from enhancing an artist's animated character to guiding a simulated inanimate object.
The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1

Spatial regulation is an important feature of signal specificity elicited by cytoplasmic tyrosine kinases of the Src family (SRC family protein tyrosine kinases [SFK]). Cholesterol-enriched membrane domains, such as caveolae, regulate association of SFK with the platelet-derived growth factor receptor (PDGFR), which is needed for kinase activation and mitogenic signaling. PAG, a ubiquitously expressed member of the transmembrane adaptor protein family, is known to negatively regulate SFK signaling though binding to Csk. We report that PAG modulates PDGFR levels in caveolae and SFK mitogenic signaling through a Csk-independent mechanism. Regulation of SFK mitogenic activity by PAG requires the first N-terminal 97 aa (PAG-N), which include the extracellular and transmembrane domains, palmitoylation sites, and a short cytoplasmic sequence. We also show that PAG-N increases ganglioside GM1 levels at the cell surface and, thus, displaces PDGFR from caveolae, a process that requires the ganglioside-specific sialidase Neu-3. In conclusion, PAG regulates PDGFR membrane partitioning and SFK mitogenic signaling by modulating GM1 levels within caveolae independently from Csk.
An Experimental Evaluation of Property Precedence in Conceptual Modelling

Interest in evaluating conceptual modelling techniques has recently experienced a revival, in part due to widespread adoption of the Unified Modelling Language (UML). In addition, the use of ontology as a framework for evaluating conceptual modelling techniques has gained acceptance. In this paper, we consider implications of applying one aspect of the ontology of Mario Bunge to conceptual modelling. Specifically, conceptual modelling has traditionally failed to provide mechanisms to indicate that some properties of types or classes may be considered dependent on others. This paper presents a theoretical rationale, using Bunge's ontological notion of precedence, for explicitly modelling such dependence in conceptual schema diagrams. We present the design of an experimental framework to test the impact of explicitly representing precedence on the ease with which a diagram can convey domain semantics. In addition, we consider how the issue of "common sense" semantics can interfere with experimental procedures to evaluate the semantics conveyed in a diagram's structure. We offer early experimental results indicating: 1) the explicit modelling of precedence improves the ability of experimental participants to verify the existence of dependence among properties (but has no effect on the ability to verify the semantics conveyed by association cardinalities); and 2) the potential for background knowledge to interfere with the semantics conveyed by diagram structure. We conclude by discussing the need for further research on both these issues.
Active Social Media Management: The Case of Health Care

Given the demand for authentic personal interactions over social media, it is unclear how much firms should actively manage their social media presence. We study this question empirically in a health care setting. We show that active social media management drives more user-generated content. However, we find that this is due to an incremental increase in user postings from an organization's employees rather than from its clients. This result holds when we explore exogenous variation in social media policies, employees, and clients that are explained by medical marketing laws, medical malpractice laws, and distortions in Medicare incentives. Further examination suggests that content being generated mainly by employees can be avoided if a firm's postings are entirely client focused. However, most firm postings seem not to be specifically targeted to clients' interests, instead highlighting more general observations or achievements of the firm itself. We show that untargeted postings like these provoke acti.
Heat flow and inferred ground surface temperature history at Tynong North, southeastern Australia

ABSTRACT Borehole temperature data have the potential to record historical variations in ground and air surface temperature, yet very few reliable, purpose-drilled, boreholes are available to explore such impacts, particularly in the southern hemisphere. The 400-m deep Tynong-1 borehole, approximately 65 km ESE of Melbourne, Australia, was drilled specifically to determine conductive heat flow and provides a unique dataset for evaluating ground surface temperature history in southeastern Australia. Steady-state conductive heat flow of 87 ± 1 mW m−2 was determined in the deeper borehole sections, with measured temperature profiles clearly demonstrating a progressive divergence of the observed temperature profile from the equilibrium model in the upper ∼150 m of the hole. We applied a Bayesian method employing a reverse jump Markov chain Monte Carlo search algorithm to explore the origins of this variation. Our results indicate a 2°C increase in ground surface temperature since 1800, after at least 500 years of relatively stable ground surface temperature. The inversion results are consistent with the trend of surface air temperature recorded in southeast Victoria by historical meteorological data since 1950. The inferred increase in ground surface temperature evident prior to 1950 is likely a cumulative effect of land clearing and a rise in surface air temperature.
Fast Asynchronous Parallel Stochastic Gradient Descent: A Lock-Free Approach with Convergence Guarantee

Stochastic gradient descent (SGD) and its variants have become more and more popular in machine learning due to their efficiency and effectiveness. To handle large-scale problems, researchers have recently proposed several parallel SGD methods for multicore systems. However, existing parallel SGD methods cannot achieve satisfactory performance in real applications. In this paper, we propose a fast asynchronous parallel SGD method, called AsySVRG, by designing an asynchronous strategy to parallelize the recently proposed SGD variant called stochastic variance reduced gradient (SVRG). AsySVRG adopts a lock-free strategy which is more efficient than other strategies with locks. Furthermore, we theoretically prove that AsySVRG is convergent with a linear convergence rate. Both theoretical and empirical results show that AsySVRG can outperform existing state-of-the-art parallel SGD methods like Hogwild! in terms of convergence rate and computation cost.