Miracle-dz/UniFAR-FTUs huggingface dataset:
Task-concurrent anodal tDCS modulates bilateral plasticity in the human suprahyoid motor cortex

Transcranial direct current stimulation (tDCS) is a non-invasive method to modulate cortical excitability in humans. Here, we examined the effects of anodal tDCS on suprahyoid motor evoked potentials (MEP) when applied over the hemisphere with stronger and weaker suprahyoid/submental projections, respectively, while study participants performed a swallowing task. Thirty healthy volunteers were invited to two experimental sessions and randomly assigned to one of two different groups. While in the first group stimulation was targeted over the hemisphere with stronger suprahyoid projections, the second group received stimulation over the weaker suprahyoid projections. tDCS was applied either as anodal or sham stimulation in a random cross-over design. Suprahyoid MEPs were assessed immediately before intervention, as well as 5, 30, 60, and 90 min after discontinuation of stimulation from both the stimulated and non-stimulated contralateral hemisphere. We found that anodal tDCS (a-tDCS) had long-lasting effects on suprahyoid MEPs on the stimulated side in both groups (tDCS targeting the stronger projections: F(1,14) = 96.2, p < 0.001; tDCS targeting the weaker projections: F(1,14) = 37.45, p < 0.001). While MEPs did not increase when elicited from the non-targeted hemisphere after stimulation of the stronger projections (F(1,14) = 0.69, p = 0.42), we found increased MEPs elicited from the non-targeted hemisphere after stimulating the weaker projections (at time points 30–90 min) (F(1,14) = 18.26, p = 0.001). We conclude that anodal tDCS has differential effects on suprahyoid MEPs elicited from the targeted and non-targeted hemisphere depending on the site of stimulation. This finding may be important for the application of a-tDCS in patients with dysphagia, for example after stroke.
Environment‐spatial conditional learning in rats with selective lesions of medial septal cholinergic neurons

Cholinergic medial septal neurons may regulate several aspects of hippocampal function, including place field stability and spatial working memory. Monkeys with damage to septal cholinergic neurons are impaired in visual‐spatial conditional learning tasks; however, this candidate function of septal cholinergic neurons has not been studied extensively in the rat. In the present study, rats with selective lesions of cholinergic neurons in the medial septum and vertical limb of the diagonal band of Broca (MS/VDB), made with 192 IgG‐saporin, were tested on a conditional associative learning task. In this task, which we term “environment‐spatial” conditional learning, the correct location of a spatial response depended on the array of local environmental cues. MS/VDB‐lesioned rats were impaired when the two parts of the conditional problem were presented concurrently, but not when one environment had been learned before the full conditional problem was presented. Our findings suggest that cholinergic MS/VDB neurons participate in some aspects of conditional associative learning in rats. They may also shed light on the involvement of cholinergic projections to the hippocampus in modulating and remodeling hippocampal spatial representations. © 2004 Wiley‐Liss, Inc.
The Invisible Hand Illusion: Multisensory Integration Leads to the Embodiment of a Discrete Volume of Empty Space

The dynamic integration of signals from different sensory modalities plays a key role in bodily self-perception. When visual information is used in the multisensory process of localizing and identifying one's own limbs, the sight of a body part often plays a dominant role. For example, it has repeatedly been shown that a viewed object must resemble a humanoid body part to permit illusory self-attribution of that object. Here, we report a perceptual illusion that challenges these assumptions by demonstrating that healthy (nonamputated) individuals can refer somatic sensations to a discrete volume of empty space and experience having an invisible hand. In 10 behavioral and one fMRI experiment, we characterized the perceptual rules and multisensory brain mechanisms that produced this “invisible hand illusion. ” Our behavioral results showed that the illusion depends on visuotactile-proprioceptive integration that obeys key spatial and temporal multisensory rules confined to near-personal space. The fMRI results associate the illusion experience with increased activity in regions related to the integration of multisensory body-related signals, most notably the bilateral ventral premotor, intraparietal, and cerebellar cortices. We further showed that a stronger feeling of having an invisible hand is associated with a higher degree of effective connectivity between the intraparietal and ventral premotor cortices. These findings demonstrate that the integration of temporally and spatially congruent multisensory signals in a premotor-intraparietal circuit is sufficient to redefine the spatial boundaries of the bodily self, even when visual information directly contradicts the presence of a physical limb at the location of the perceived illusory hand.
GlobalEd : A Comparison of Leadership in FTF and CMC Environments Running head : Leadership in FTF and CMC Environments

The GlobalEd Project, an international negotiations simulation, helps middle and high school students understand the components of cross-cultural communication, the role of precision in negotiation, the dynamics of leadership and group decision-making, and the complexities of the global system. As students collaborated within their classroom setting and across classrooms through synchronous and asynchronous communication while negotiating various international issues, leaders emerged within and across the countries that they played. This study examined high school students’ perceptions of leadership traits across mediums in face-to-face (FTF) and computermediated communication (CMC) environments, across time over the course of a fiveweek simulation, and across gender groupings of all male, all female, and mixed-gender groups. Results indicated that leadership attributes did not vary across contexts, but the strength by which they were assessed varied across contexts. Leadership in FTF and CMC Environments Johnson et al., 2006 3 GlobalEd: A Comparison of Leadership in FTF and CMC Environments
The CASA Trauma and Attachment Group (TAG) Program for Children who have Attachment Issues Following Early Developmental Trauma.

OBJECTIVE There is relatively little research about effective therapeutic approaches for children in middle childhood who have attachment related diagnoses as a result of experiencing significant, early developmental trauma. This study describes findings from an intensive, dyad-based intervention, aimed at stabilizing attachment relationships with primary caregivers, increasing caregiver reflective function skills, and reducing children's trauma-related behavioural sequelae. METHOD We analyzed retrospective data from 51 caregiver/child dyads who participated in the Trauma and Attachment Group (TAG) Program from September 2011-December 2014. This data included pre- and post-intervention scores retrieved from the Parenting Relationship Questionnaire (PRQ), the Parent Report of Post-Traumatic Stress Symptoms (PROPS), and the Parental Reflective Functioning Questionnaire (PRFQ-1). RESULTS The preliminary findings show statistically significant improvements in attachment, communication, discipline practices, involvement, and relational frustration. Additionally there were statistically significant improvements in parental reflective functioning, and a trend towards a reduction in symptoms typical of post-traumatic stress disorder. CONCLUSION Poor quality or inconsistent interactions with early caregivers can lead to life-long impairments in physical and mental health. This intensive program shows potential as a way to improve longer-term outcomes for children exposed to early developmental trauma. Longer-term research is required to further substantiate outcomes, appraise cost analysis, as well as to consider evaluation with appropriate comparison groups.
Alcohol metabolism and oesophageal cancer: a systematic review of the evidence

Alcohol is a major risk factor for oesophageal squamous cell carcinoma (OSCC), the most prevalent histological subtype of oesophageal cancer (OC) worldwide. The metabolism of alcohol is regulated by specific enzymes whose activity and expression is influenced by genetic polymorphisms. We conducted a systematic review of current epidemiological evidence of the relationship between alcohol intake and OC risk, including the role of tobacco smoking and functional polymorphisms of alcohol dehydrogenases (ADHs) and aldehyde dehydrogenases (ALDHs). Potential biological mechanisms underlying oesophageal carcinogenesis are also discussed. Frequency and intensity of alcohol intake have been consistently associated with an increased risk of OSCC in regions with low and high incidence of the disease. The highest risk was reported among tobacco smokers, whereas the association between alcohol and OSCC risk was weak in the absence of tobacco use. The ADH1B, ADH1C and ALDH2 gene polymorphisms influence the risk of OSCC through modulation of acetaldehyde metabolism and propensity to alcohol intake. These functional variants may be suitable proxies of alcohol exposure for use in Mendelian randomization studies if complemented by reported alcohol intake data. Recent epidemiological and experimental studies investigating the role of alcohol consumption in OC development have implicated the microbiome as a new promising avenue for research, which entail novel potential mechanisms of alcohol-related oesophageal carcinogenesis. Microbial communities associated with alcohol consumption might be used as biomarkers to raise the potential of intervening among susceptible individuals.
A Limited Role for Carbonic Anhydrase in C4 Photosynthesis as Revealed by a ca1ca2 Double Mutant in Maize1[W][OPEN]

Maize plants with significantly reduced carbonic anhydrase activity have impaired growth at subambient CO2, but photosynthesis in these plants is not limited under current atmospheric conditions. Carbonic anhydrase (CA) catalyzes the first biochemical step of the carbon-concentrating mechanism of C4 plants, and in C4 monocots it has been suggested that CA activity is near limiting for photosynthesis. Here, we test this hypothesis through the characterization of transposon-induced mutant alleles of Ca1 and Ca2 in maize (Zea mays). These two isoforms account for more than 85% of the CA transcript pool. A significant change in isotopic discrimination is observed in mutant plants, which have as little as 3% of wild-type CA activity, but surprisingly, photosynthesis is not reduced under current or elevated CO2 partial pressure (pCO2). However, growth and rates of photosynthesis under subambient pCO2 are significantly impaired in the mutants. These findings suggest that, while CA is not limiting for C4 photosynthesis in maize at current pCO2, it likely maintains high rates of photosynthesis when CO2 availability is reduced. Current atmospheric CO2 levels now exceed 400 ppm (approximately 40.53 Pa) and contrast with the low-pCO2 conditions under which C4 plants expanded their range approximately 10 million years ago, when the global atmospheric CO2 was below 300 ppm (approximately 30.4 Pa). Thus, as CO2 levels continue to rise, selective pressures for high levels of CA may be limited to arid climates where stomatal closure reduces CO2 availability to the leaf.
Word structure and decomposition effects in reading

Theories on the processing of compound words differ on the role attributed to access to individual constituents. These theories are mostly based on empirical evidence obtained in experimental settings that could induce artificial effects normally not occurring in natural processing. In this study we investigated the processing of compounds as compared to noncompound complex words in Italian through a reading task with eye movement recording. We included both head-initial and head-final compounds, in order to test whether the position of the head may influence the reading process. After ruling out the effects of length and frequency, we observed that pseudocompounds (i.e., words with a segment homograph to a real word in the leftmost part) elicited longer total reading times than all other types of complex words, including compounds. Furthermore, head-final compounds elicited longer total reading times than head-initial compounds. The results suggest that a word structure resembling a compound may induce longer processing, presumably related to unexpected morphological structures. The results also converge with previous evidence that in some cases there is a higher processing costs for head-final as opposed to head-initial compounds, possibly indexing a reanalysis of the stimulus in order to correctly assign the constituent properties. However, a deeper analysis restricted to compounds revealed a more complex scenario where several variables interact with headedness (namely, first and second constituent frequency, compound frequency, and compound length), and future studies are needed to discriminate among possible interpretations. Overall, our findings suggest that longer reading times are related to solving incongruities due to noncanonical structures, rather than to morphologically complexity per se.
Time to stop giving indiscriminate massive doses of synthetic vitamin A to Indian children

Vitamin A deficiency is now uncommon Vitamin A deficiency was a major public health problem in India in the 1950s and 1960s. Clinically evident deficiency is common only in some districts. Severe deficiency, causing corneal xerophthalmia or keratomalacia and eventual blindness in children, is now uncommon. Clinical signs of mild xerophthalmia such as Bitot’s spots and night blindness, still seen in children from deprived communities, are declining. The first repeat survey of the Indian National Nutrition Monitoring Bureau carried out in the same villages showed that prevalence of Bitot’s spots had declined in 1988–90 compared with 1975–9, from 1? 8% to 0? 7%. A survey of the Indian Council of Medical Research in 1988, covering sixteen districts mostly in northern and eastern regions, showed that prevalence of Bitot’s spots ranged from 0 to 4? 7% and that of night blindness from 0? 4 to 4? 8%. Prevalence rates vary widely between and also within states. The context is general improvement of child health in India. Prevalence of severe protein–energy malnutrition dropped from 15 % in 1975 to less than 2 % in 2000. Immunisation coverage for measles and other vaccinepreventable diseases has improved from 5–7 % in the early 1970s to the current 80–90 %. The national government of India has addressed the issue of food insecurity diligently, one result being significant improvement in intake of foods rich in vitamin A.
Pattern Formation and Emergence of Collective Behavior in Bacterial Growth

Collective surface migration of bacterial cells on a growth medium can lead to interesting spatiotemporal patterns and dynamical behaviors. Studying the rich behavior of these swarming colonies can provide insight in how the multicellular organisms were originally formed. Bacterial swarming is an example of emergent behavior as a result of interplay between the collective interactions of a population of bacterial cells, colony growth, and hydrodynamic motions. Several models have been proposed to describe bacterial swarming some of which can qualitatively reproduce a variety of colony shapes observed in experiments. In this paper, some experimental work along with a theoretical model for the mechanism of pattern formation in bacterial swarming that captures some qualitative and quantitative aspects of this phenomenon is reviewed.
Distinct Pathways for Tumor Necrosis Factor Alpha and Ceramides in Human Cytomegalovirus Infection

ABSTRACT Human cytomegalovirus (HCMV) infection can be fatal to immunocompromised individuals. We have previously reported that gamma interferon and tumor necrosis factor alpha (TNF-α) synergistically inhibit HCMV replication in vitro. Ceramides have been described as second messengers induced by TNF-α. To investigate the mechanisms involved in the inhibition of HCMV by TNF-α, in the present study we have analyzed ceramide production by U373 MG astrocytoma cells and the effects of TNF-α versus ceramides on HCMV replication. Our results show that U373 MG cells did not produce ceramides upon incubation with TNF-α. Moreover, long-chain ceramides induced by treatment with exogenous bacterial sphingomyelinase inhibited HCMV replication in synergy with TNF-α. Surprisingly, short-chain permeant C6-ceramide increased viral replication. Our results show that the anti-HCMV activity of TNF-α is independent of ceramides. In addition, our results suggest that TNF-α and endogenous long-chain ceramides use separate pathways of cell signalling to inhibit HCMV replication, while permeant C6-ceramide appears to activate a third pathway leading to an opposite effect.
Asymmetric division triggers cell-specific gene expression through coupled capture and stabilization of a phosphatase

Formation of a division septum near a randomly chosen pole during sporulation in Bacillus subtilis creates unequal sized daughter cells with dissimilar programs of gene expression. An unanswered question is how polar septation activates a transcription factor (σF) selectively in the small cell. We present evidence that the upstream regulator of σF, the phosphatase SpoIIE, is compartmentalized in the small cell by transfer from the polar septum to the adjacent cell pole where SpoIIE is protected from proteolysis and activated. Polar recognition, protection from proteolysis, and stimulation of phosphatase activity are linked to oligomerization of SpoIIE. This mechanism for initiating cell-specific gene expression is independent of additional sporulation proteins; vegetative cells engineered to divide near a pole sequester SpoIIE and activate σF in small cells. Thus, a simple model explains how SpoIIE responds to a stochastically-generated cue to activate σF at the right time and in the right place. DOI: http://dx.doi. org/10.7554/eLife.08145. 001
Using Machine Learning to Detect Noisy Neighbors in 5G Networks

5G networks are expected to be more dynamic and chaotic in their structure than current networks. With the advent of Network Function Virtualization (NFV), Network Functions (NF) will no longer be tightly coupled with the hardware they are running on, which poses new challenges in network management. Noisy neighbor is a term commonly used to describe situations in NFV infrastructure where an application experiences degradation in performance due to the fact that some of the resources it needs are occupied by other applications in the same cloud node. These situations cannot be easily identified using straightforward approaches, which calls for the use of sophisticated methods for NFV infrastructure management. In this paper we demonstrate how Machine Learning (ML) techniques can be used to identify such events. Through experiments using data collected at real NFV infrastructure, we show that standard models for automated classification can detect the noisy neighbor phenomenon with an accuracy of more than 90% in a simple scenario.
Evasin‐displaying lactic acid bacteria bind different chemokines and neutralize CXCL8 production in Caco‐2 cells

Chemokines are key signals in the immune system and play an important role as proinflammatory mediators in the pathology of inflammatory bowel disease and colorectal cancer, making them an important target for therapy. Recombinant lactic acid bacteria (LAB) were engineered to bind CC and CXC chemokines by displaying chemokine‐binding proteins evasin‐1, evasin‐3 and evasin‐4 on their surface. Evasin genes were cloned into lactococcal surface display vector and overexpressed in L. lactis NZ9000 and NZ9000ΔhtrA in fusion with secretion signal and surface anchor. Evasin‐displaying bacteria removed from 15% to 90% of 11 different chemokines from the solution as determined with ELISA and Luminex multiplexing assays, whereby L. lactis NZ9000ΔhtrA proved more efficient. Lactobacillus salivarius ATCC 11741 was coated with L. lactis‐expressed evasin fusion protein, and its ability to bind chemokines was also confirmed. Evasin‐3‐displaying L. lactis removed 76.0% of IL‐1β‐induced CXCL8 from the supernatant of Caco‐2 epithelial cells. It also prevented secretion of CXCL8 from Caco‐2 cells in a time‐dependent manner when added before induction with IL‐1β. Evasin‐displaying LAB have the ability to bind multiple chemokines simultaneously and exert synergistic activity. This innovative treatment approach therefore has the potential for mucosal therapy of inflammatory bowel disease or colorectal cancer.
Intrarenal hemodynamic changes after captopril test in patients with type 2 diabetes: a duplex Doppler sonography study.

OBJECTIVE ACE inhibitors are known to be effective in preventing the progression of diabetic nephropathy. Activation of the renin-angiotensin system (RAS) is reported to contribute to intrarenal hemodynamic abnormality in diabetic patients. We examined whether RAS blockade by captopril induces intrarenal hemodynamic changes in normotensive patients with type 2 diabetes. RESEARCH DESIGN AND METHODS The patients ranged in age from 40 to 65 years (20 men and 20 women). A total of 15 age- and sex-matched healthy individuals served as control subjects. Resistive index (RI) of interlobar arteries was examined by duplex Doppler sonography before and after the oral captopril (25 mg) test. RESULTS At baseline, no significant differences in RI values or plasma renin activity (PRA) were seen between the patients and healthy subjects. In healthy subjects, the RI values after the captopril test were significantly higher than baseline values (P < 0.01). However, in patients with type 2 diabetes, both with normoalbuminuria and microalbuminuria, RI values after the test were significantly lower than baseline values (P < 0.001). There were significant negative correlations between DeltaRI value and HbA1c (r = -0.458, P < 0.005) and between DeltaRI value and baseline PRA in diabetic patients (r = -0.339, P < 0.05). Multiple regression analysis showed that HbA1c and baseline PRA significantly and independently affected the magnitude of decrease in RI values after captopril administration in diabetic patients (R2 = 0.391, P < 0.0001). CONCLUSIONS These results indicate that the intrarenal RAS may be activated in diabetic patients, that such activation may be affected by poor glycemic control, and that blockade of RAS activation by ACE inhibitor reduces intrarenal vascular resistance in diabetic patients. The results emphasize the beneficial effects of ACE inhibition in improving intrarenal hemodynamics in diabetic patients.