Miracle-dz/UniFAR-FTUs huggingface dataset:
The presequence of a chimeric construct dictates which of two mechanisms are utilized for translocation across the thylakoid membrane: evidence for the existence of two distinct translocation systems.

The translocation of plastocyanin across the thylakoid membrane in Pisum sativum has been studied in reconstitution assays and using chimeric constructs. The reconstitution assays demonstrate that plastocyanin translocation is absolutely dependent on the presence of a stromal factor(s) and nucleotide triphosphates (NTPs), whereas neither element is required for the translocation of the 23 or 16 kDa proteins of the oxygen‐evolving complex. Previous studies had revealed that the transthylakoidal delta pH is essential for translocation of the 23 and 16 kDa proteins but unnecessary for plastocyanin translocation. The basis for these mechanistic differences has been tested by analysing the translocation of a chimeric construct consisting of the presequence of the 23 kDa protein linked to the mature plastocyanin sequence. This construct is efficiently imported into thylakoids in the absence of stromal extracts or NTPs and translocation across the thylakoid membrane within intact chloroplasts is totally inhibited by the uncoupler nigericin: the translocation requirements are thus identical to those of the pre‐23 kDa protein and diametrically opposite to those of pre‐plastocyanin. Transport across the thylakoid membrane of a second fusion protein, consisting of the presequence of the 16 kDa protein linked to mature plastocyanin, is also dependent on a delta pH. The data suggest that two distinct systems are involved in the translocation of proteins across the thylakoid membrane, with each system recognizing specific signals within the presequences of a subset of lumenal protein precursors.
Promoting Reconciliation Aer Genocide and Mass Killing in Rwanda—and Other Postconict Settings: Understanding the Roots of Violence, Healing, Shared History, and General Principles

In the a ermath of genocide, mass killing, or intractable—persistent and violent—con$ ict between groups, how can members of these groups continue to live together and build a nonviolent future.1 How can they create reasonably harmonious relations? Increasingly, research and observations have shown that when violent con$ icts end, even if they end by a peace agreement—rather than one party defeating another as in Rwanda, which will be a focus of this chapter—violence is likely to recur (de la Rey, 2001; Lederach, 1997). 4 e peace agreement may not 5 t the interests of all the segments of the population or elites. Even more importantly, the end to the violence is not likely to change the hostility that gave rise to violence in the 5 rst place, and has become more intense in the course of and as a result of the violence. I will describe here an approach my associates and I have developed to promote reconciliation between Tutsis and Hutus in the a ermath of the horri5 c genocide in
Hierarchical nanoflake-assembled flower-like NiCo double hydroxide@NiC2O4 microspheres for high-performance supercapacitor

ABSTRACT Assembly of NiCo double hydroxide@NiC2O4 porous microspheres on Ni foam was fabricated by the direct anodisation of nickel foam (NF) and the subsequent electrodeposition of NiCo double hydroxide (NiCo-DH). The obtained Ni foam-supported NiCo-DH@NiC2O4 (NiCo-DH@NiC2O4/NF) was used as the binder-free electrode for electrochemical capacitor (EC). The combination of NiC2O4 and NiCo-DH increases the amount of active sites for redox reactions while the hierarchical porous morphology significantly enhances the diffusion of electrolyte. With Ni foam as 3D conductive substrate and current collector, the electronic conductivity of the electrode material was significantly improved and the diffusion of the electrolyte on the electrode surface was effectively enhanced. NiCo-DH@NiC2O4/NF demonstrates a high areal capacity, a good cycling stability and a good rate capability . In addition, a hybrid supercapacitor (HSC) assembled with NiCo-DH@NiC2O4/NF as the positive electrode and activated carbon (AC) as the negative electrode exhibited good performance with high energy density and power density.
Involvement of DNA Damage Response Pathways in Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) has been known as one of the most lethal human malignancies, due to the difficulty of early detection, chemoresistance, and radioresistance, and is characterized by active angiogenesis and metastasis, which account for rapid recurrence and poor survival. Its development has been closely associated with multiple risk factors, including hepatitis B and C virus infection, alcohol consumption, obesity, and diet contamination. Genetic alterations and genomic instability, probably resulted from unrepaired DNA lesions, are increasingly recognized as a common feature of human HCC. Dysregulation of DNA damage repair and signaling to cell cycle checkpoints, known as the DNA damage response (DDR), is associated with a predisposition to cancer and affects responses to DNA-damaging anticancer therapy. It has been demonstrated that various HCC-associated risk factors are able to promote DNA damages, formation of DNA adducts, and chromosomal aberrations. Hence, alterations in the DDR pathways may accumulate these lesions to trigger hepatocarcinogenesis and also to facilitate advanced HCC progression. This review collects some of the most known information about the link between HCC-associated risk factors and DDR pathways in HCC. Hopefully, the review will remind the researchers and clinicians of further characterizing and validating the roles of these DDR pathways in HCC.
Contribution of problem drug users’ deaths to excess mortality in Scotland: secondary analysis of cohort study

Objectives To examine the “Scottish effect”—namely, the growing divergence between mortality in Scotland and England that is not explained by national differences in levels of deprivation—and, more specifically, to examine the extent to which the Scottish effect is explained by cross national differences in the prevalence of problem drug use. Design Secondary analysis of cohort study (the DORIS study). Participants 1033 Scottish drug users recruited to the cohort study in 33 drug treatment facilities across Scotland in 2001-2 and followed up 33 months later in 2004-5. Results 38 deaths occurred in the cohort, giving a standardised mortality ratio for the cohort of 1244 (95% credible interval 876 to 1678). Only 22 of the 38 deaths in drug users were classified as drug related deaths. From estimates of the size of the problem drug using populations in both England and Scotland, the contribution of deaths in drug users to national death rates can be estimated: the attributable risk fraction for Scotland is 17.3% (12.3% to 22.8%) and that for England is 11.1% (7.8% to 14.8%). Excluding estimated numbers of deaths in drug users would bring down age standardised mortality at ages 15-54 years from 196 to 162 per 100 000 in Scotland and from 138 to 122 per 100 000 in England; 32.0% (22.3% to 43.0%) of the excess mortality in Scotland is due to drug use. Conclusion Although problem drug use is a low prevalence risk behaviour, it carries a high mortality; the standardised mortality ratio for Scottish drug users is 12 times as high as for the general population. The higher prevalence of problem drug use in Scotland than in England accounts for a third of Scotland’s excess mortality over England. Successful public health efforts to reduce the prevalence of problem drug use in Scotland or deaths in Scottish drug users would have a dramatic impact on overall mortality in Scotland.
What have we learned about who conserves soil

WHAT kinds of farmers care the most about conserving soil? Which ones actually do the most about it? What farm characteristics are most conducive to conservation? How important are various information sources in shaping farmers' soil conservation attitudes and practices? These questions have interested conservationists and agricultural policymakers since the start of the soil conservation movement, and they have been the subject of a substantial body of research, primarily by agricultural economists and rural sociologists. Further efforts to answer these questions are fully justified on two counts: Soil loss has long been a major national environmental problem, but its resolution remains frustratingly beyond our grasp. The failure is not primarily technical. Available techniques continue to advance, but that does not mean that farmers necessarily use them. Farmers must want to use them, and their farms' circumstances must permit them to use them (7, 33). Concern is growing about agricultural resource and environmental problems related to, but broader than, traditional soil conservation issues. Examples include contamination of groundwater by pesticides and fertilizers, loss of wildlife habitat, and toxic …
Evidence for Edge Enhancements of Soil Respiration in Temperate Forests

Forest fragmentation impacts carbon uptake and storage; however, the magnitude and direction of fragmentation impacts on soil respiration remain poorly characterized. We quantify soil respiration rates along edge‐to‐interior transects in two temperate broadleaf forests in the eastern United States that vary in climate, species composition, and soil type. We observe average soil respiration rates 15–26% higher at the forest edge compared to the interior, corresponding to large gradients in soil temperature. We find no significant difference in the sensitivity of soil respiration to temperature between the forest edge and interior. Fragmentation and resultant shifts in microenvironment alter forest productivity and soil respiration near forest edges. Ecosystem models do not currently represent edge dynamics, but given the prevalence of landscape fragmentation and its effect on carbon cycling along forest edges, this omission likely introduces an important source of uncertainty in our understanding of terrestrial carbon dynamics with a changing landscape and climate.
Brain activity-based image classification from rapid serial visual presentation

— We report the design and performance of a brain-computer interface (BCI) system for real-time single-trial binary classification of viewed images based on participant-specific dynamic brain response signatures in high-density (128-channel) electroencephalographic (EEG) data acquired during a rapid serial visual presentation (RSVP) task. Image clips were selected from a broad area image and presented in rapid succession (12/s) in 4.1-s bursts. Participants indicated by subsequent button press whether or not each burst of images included a target airplane feature. Image clip creation and search path selection were designed to maximize user comfort and maintain user awareness of spatial context. Independent component analysis (ICA) was used to extract a set of independent source time-courses and their minimally-redundant low-dimensional informative features in the time and time-frequency amplitude domains from 128-channel EEG data recorded during clip burst presentations in a training session. The naive Bayes fusion of two Fisher discriminant classifiers, computed from the 100 most discriminative time and time-frequency features respectively, was used to estimate the likelihood that each clip contained a target feature. This estimator was applied online in a subsequent test session. Across 8 training/test session pairs from 7 participants, median area under the receiver operator characteristic (ROC) curve, by tenfold cross-validation, was 0.97 for within-session and 0.87 for between-session estimates, and was nearly as high (0.83) for targets presented in bursts that participants mistakenly reported to include no target features.
High-Dimensional Indexing by Sparse Approximation

In this paper we propose a high-dimensional indexing technique, based on sparse approximation techniques to speed up the search and retrieval of similar images given a query image feature vector. Feature vectors are stored on an inverted indexed based on a sparsifying dictionary for l0 regression, optimized to reduce the data dimensionality. It concentrates the energy of the original vector on a few coefficients of a higher dimensional representation. The index explores the coefficient locality of the sparse representations, to guide the search through the inverted index. Evaluation on three large-scale datasets showed that our method compares favorably to the state-of-the-art. On a 1 million dataset of SIFT vectors, our method achieved 60.8% precision at 50 by inspecting only 5% of the full dataset, and by using only 1/4 of the time a linear search takes.
Vitamin E and essential polyunsaturated fatty acids supplementation in schizophrenia patients treated with haloperidol

Objectives: Previously, oxidative damage has been associated with severity of clinical symptoms and supplementation with antioxidants and essential polyunsaturated fatty acids (EPUFAs) was proposed to have beneficial effects in schizophrenia. We evaluated the effects of supplementation with EPUFAs and vitamin E in patients treated with haloperidol depot injection. Design: This was a double-blind randomized placebo-controlled study with four arms (Placebo, vitamin E, EPUFAs, and vitamin E + EPUFAs). Biomarkers of oxidative stress, neurochemistry, psychopathology, and extrapyramidal symptoms were assessed at baseline and after 4 months. Results: In EPUFAs group of patients, reduced glutathione concentration was increased compared to placebo. Concentration of oxidized glutathione was decreased in patients receiving vitamin E. In addition, compared to placebo a non-significant trend of increased activity of catalase and superoxide dismutase was observed in all three treatment groups. Patients receiving vitamin E experienced less motor retardation. No difference in extrapyramidal symptoms was found. Discussion: Our study indicates that supplementation with vitamin E and EPUFAs may improve the antioxidative defense, especially glutathione system, while there is no major effect on symptoms severity. Supplemental treatment with EPUFAs and vitamin E in schizophrenia patients treated with haloperidol is potentially beneficial and a larger independent study appears warranted.
Articaine (4%) with epinephrine (1:100,000 or 1:200,000) in inferior alveolar nerve block: Effects on the vital signs and onset, and duration of anesthesia

Background This prospective, randomized, double-blind, clinical study was conducted to compare the effects of 4% articaine with 1:100,000 epinephrine (A100) and 4% articaine with 1:200,000 epinephrine (A200) on the vital signs and onset and duration of anesthesia in an inferior alveolar nerve block (IANB). Methods In the first appointment, an IANB was performed by injecting A100 or A200 in 1 side of the mouth (right or left) randomly in patients referred for extraction of both their first mandibular molars. In the second appointment, the protocol was repeated and the other anesthetic solution was injected in the side that had not received the block in the previous session. Systolic and diastolic blood pressures (SBP and DBP) and pulse rate were measured during and 5 min after the injection. The onset and duration of anesthesia were also evaluated. Data were analyzed using t-test and Mann-Whitney U-test, and p-value was set at 0.05. Results SBP and pulse rate changes were slightly more with A100; however, DBP changes were more with A200, although the differences were not significant (P > 0.05). There were no statistically significant differences in the parameters evaluated in this study. The onset and duration of anesthesia, and the changes in SBP, DBP, and pulse rate during and 5 min after the injection were the same in both the groups. Conclusions For an IANB, A200 and A100 were equally efficient and successful in producing the block. Epinephrine concentration did not influence the effects of 4% articaine.
A framework for less than best effort congestion control with soft deadlines

Applications like inter data-centre synchronisation or client-to-cloud backups require a reliable end-to-end data transfer, however, they typically do not have strong capacity or latency constraints, just a loose delivery deadline. Besides, their potential to disrupt more quality-constrained flows should be kept to a minimum. These applications could be well served by a transport protocol providing a less-than-best-effort (LBE) or scavenger service rather than TCP but, neither TCP nor standard LBE methods like LEDBAT consider any notion of deadline or completion time. TCP simply tries to maximise the use of available capacity, while LEDBAT tries to enforce an LBE behaviour regardless of any timeliness requirements. This paper introduces a framework for adding both LBE behaviour and awareness of “soft” delivery deadlines to any congestion control (CC) algorithm, whether loss-based, delay-based or explicit signaling-based. This effectively allows it to turn an arbitrary CC protocol into a scavenger protocol that dynamically adapts its sending rate to network conditions and remaining time before the deadline, to balance timeliness and transmission aggressiveness. Network utility maximization (NUM) theory provides a solid foundation for the proposal. The effectiveness of the approach is validated by numerical and simulation experiments, with TCP Cubic and Vegas used as examples.
Tyrosine phosphorylation of the Kv1.3 potassium channel

Kv1.3, a voltage-dependent potassium channel cloned from mammalian brain and T lymphocytes, contains multiple tyrosine residues that are putative targets for tyrosine kinases. We have examined the tyrosine phosphorylation of Kv1.3, expressed transiently in human embryonic kidney (or HEK) 293 cells, by endogenous and coexpressed tyrosine kinases. Tyrosine phosphorylation is measured by a strategy of immunoprecipitation followed by. Western blot analysis, using antibodies that specifically recognize Kv1.3 and phosphotyrosine. Coexpression of the constitutively active tyrosine kinase v-src, together with Kv1.3, causes a large increase in the tyrosine phosphorylation of the channel protein. This phosphorylation of Kv1.3 can be reversed by treatment with alkaline phosphatase before Western blot analysis. Coexpression with a receptor tyrosine kinase, the human epidermal growth factor receptor, also causes an increase in tyrosine phosphorylation of Kv1.3. The effects of endogenous tyrosine kinases were examined by treating Kv1.3-transfected cells with the specific membrane-permeant tyrosine phosphatase inhibitor pervanadate. Pervanadate treatment causes a time- and concentration-dependent increase in the tyrosine phosphorylation of Kv1.3. This increased tyrosine phosphorylation of Kv1.3 is accompanied by a time-dependent decrease in Kv1.3 current, measured by patch-clamp analysis with cell- attached membrane patches. The pervanadate-induced suppression of current and much of the channel tyrosine phosphorylation are eliminated by mutation of a specific tyrosine residue, at position 449 of Kv1.3, to phenylalanine. Thus, there is a continual phosphorylation and dephosphorylation of Kv1.3 by endogenous kinases and phosphatases, and perturbation of this constitutive phosphorylation/dephosphorylation cycle can profoundly influence channel activity.
PD-1-Mediated Suppression of IL-2 Production Induces CD8+ T Cell Anergy In Vivo1

Accumulating evidence suggests that PD-1, an immuno-inhibitory receptor expressed on activated T cells, regulates peripheral T cell tolerance. In particular, PD-1 is involved in the induction and/or maintenance of T cells’ intrinsic unresponsiveness to previously encountered Ags, although the mechanism is yet to be determined. We used a simple experimental model to dissect the mechanism for anergy establishment, in which 2C TCR transgenic rag2−/− PD-1+/+ mice were anergized by a single injection of a cognate peptide. Interestingly, 2C rag2−/− PD-1−/− mice were totally resistant to anergy induction by the same treatment; thus, PD-1 was responsible for anergy induction. Furthermore, PD-1 expression was induced within 24 h of the initial Ag exposure. The establishment of anergy was associated with a marked down-regulation of IL-2 from the CD8+ T cells. In fact, IL-2 blockade resulted in anergy even in 2C rag2−/−PD-1−/− T cells. Furthermore, the complementation of the IL-2 signal in 2C rag2−/− PD-1+/+ mice reversed the anergy induction. We propose that CD8+ T cell anergy is induced by a reduction of cell-autonomous IL-2 synthesis, which is caused by the quick expression of PD-1 in response to Ag stimulation and the subsequent stimulation of this receptor by its ligands on surrounding cells.
Beyond developable

We present a computational method for interactive 3D design and rationalization of surfaces via auxetic materials, i.e., flat flexible material that can stretch uniformly up to a certain extent. A key motivation for studying such material is that one can approximate doubly-curved surfaces (such as the sphere) using only flat pieces, making it attractive for fabrication. We physically realize surfaces by introducing cuts into approximately inextensible material such as sheet metal, plastic, or leather. The cutting pattern is modeled as a regular triangular linkage that yields hexagonal openings of spatially-varying radius when stretched. In the same way that isometry is fundamental to modeling developable surfaces, we leverage conformal geometry to understand auxetic design. In particular, we compute a global conformal map with bounded scale factor to initialize an otherwise intractable non-linear optimization. We demonstrate that this global approach can handle non-trivial topology and non-local dependencies inherent in auxetic material. Design studies and physical prototypes are used to illustrate a wide range of possible applications.
Magnetic Resonance Characterization of Porous Media Using Diffusion through Internal Magnetic Fields

When a porous material is inserted into a uniform magnetic field, spatially varying fields typically arise inside the pore space due to susceptibility contrast between the solid matrix and the surrounding fluid. As a result, direct measurement of the field variation may provide a unique opportunity to characterize the pore geometry. The sensitivity of nuclear magnetic resonance (NMR) to inhomogeneous field variations through their dephasing effects on diffusing spins is unique and powerful. Recent theoretical and experimental research sheds new light on how to utilize susceptibility-induced internal field gradients to quantitatively probe the microstructure of porous materials. This article reviews ongoing developments based on the stimulated echo-pulse sequence to extend the characterization of porous media using both spatially resolved and unresolved susceptibility-induced internal gradients that operate on a diffusing-spin ensemble.
Asymptotically Optimal Resource Allocation in OFDM-Based Cognitive Networks with Multiple Relays

In this letter, the problem of resources allocation in decode-and-forward (DF) relayed OFDM based cognitive system is considered. The dual decomposition technique is adopted to obtain an asymptotically optimal subcarrier pairing, relay selection, and power allocation. The resources are optimized under the individual power constraints in source and relays so that the sum rate is maximized while the interference induced to the primary system is kept below a pre-specified interference temperature limit. Moreover, a sub-optimal scheme is presented to avoid the high computational complexity of the optimal scheme. The sub-optimal algorithm allocates jointly the different resources taking into account the channel qualities, the DF-relaying strategy, the interference induced to the primary system, and the individual power budgets. The performance of the different schemes and the impact of the constraints values are discussed through the numerical simulation results.