Miracle-dz/UniFAR-FTUs huggingface dataset:
Award Number: DAMD17-03-1-0522 TITLE: Constitutive Activation of NF-Κb in Prostate Carcinoma Cells through a Positive Feedback Loop: Implication of Inducible IKK-Related Kinase (Ikki)

s presented at the national meetings: 1. Yemelyanov A. , Kobzeva V. , Budunova I. Role of IKKi in prostate cancer: A link between inflammation and androgen receptor signaling. Proceedings of AACR, 2007 (abstract # LBA9158). 2. Budunova I. , Yemelyanov A. , Gasparian A. Role of IKKs and transcription factor NF-kB in prostate tumorigenesis. P. IMPACT DOD meeting, 2007, Atlanta. Seminars presented by P.I. Compound A, a novel phyto-modulator of steroid hormone receptors, as a candidate for prostate cancer therapy. Tumor cell biology seminars. R. Lurie Cancer Center. NU. April, 2007. Non-steroidal modulators of steroid hormone receptors as candidates for prostate cancer therapy. Children’s Memorial Research Center, Chicago. May , 2007 Conclusions for FY04. Our data provide experimental evidence that IKKi could be involved in the regulation of NF-kB activity in PC cells through a positive feedback loop. For example, NF-kB was constitutively activated in PC cells stably infected with w.t. IKK-expressing lentivirus. IKKi –infected cells were more responsive to different NF-kB inducers. IKKi is highly expressed in androgen-independent malignant PC cell lines. The
Chromatin reader L(3)mbt requires the Myb–MuvB/DREAM transcriptional regulatory complex for chromosomal recruitment

Significance Histone binding proteins are critical for chromosome function, and mechanisms targeting them to nucleosomes are crucial. The Drosophila tumor suppressor L(3)mbt binds to methylated lysines of nucleosomal histones repressing gene transcription. We show that L(3)mbt chromosomal targeting requires proteins of a site-specific DNA binding complex [Myb–MuvB (MMB)/DREAM] and Mip120, an MMB/DREAM core component, is critical for recruitment. Surprisingly, chromosome association of L(3)mbt is insufficient for repression, as other MMB/DREAM members are required for L(3)mbt-mediated repression but not its chromosome targeting. Loss of l(3)mbt leads to lethal malignant brain tumors. We discuss our findings in the context of complex mechanisms where specific genes activated by loss of l(3)mbt may help tumor progression, whereas deletion of MMB genes may suppress this phenotype. Lethal malignant brain tumors (lmbt) result from the loss of the conserved transcriptional repressor l(3)mbt, in Drosophila melanogaster. Similar mutations in the human homolog L3MBTL1 correlate with some cancers. The protein’s C-terminal MBT repeats bind mono and dimethylated histones in vitro, which could influence recruitment of L3MBTL1 to its target sites. The L(3)mbt chromatin targeting mechanism, however, is controversial and several studies suggest insufficiency or a minor role for histone methylation in determining the site specificity for recruitment. We report that L(3)mbt colocalizes with core members of the Myb–MuvB/DREAM (MMB/DREAM) transcriptional regulatory complex genome-wide, and that L(3)mbt-mediated repression requires this complex in salivary glands and larval brains. Loss of l(3)mbt or of MMB components through mutation cause similar spurious expression of genes, including the transposon regulatory gene piwi, in terminally differentiated cells. The DNA-binding MMB core component Mip120 (Lin54) is required for L(3)mbt recruitment to chromosomes, whereas Mip130 (Lin9) (an MMB core protein) and E2f2 (an MMB transcriptional repressor) are not, but are essential for repression. Cytolocalization experiments suggest the presence of site-specific differential composition of MMB in polytene chromosomes where some loci were bound by a Myb-containing or alternatively, an E2f2 and L(3)mbt form of the complex.
Molecular Characterization of Three GIBBERELLIN-INSENSITIVE DWARF2 Homologous Genes in Common Wheat

F-box protein is a core component of the ubiquitin E3 ligase SCF complex and is involved in the gibberellin (GA) signaling pathway. To elucidate the molecular mechanism of GA signaling in wheat, three homologous GIBBERELLIN-INSENSITIVE DWARF2 genes, TaGID2s, were isolated from the Chinese Spring wheat variety. A subcellular localization assay in onion epidermal cells and Arabidopsis mesophyll protoplasts showed that TaGID2s are localized in the nuclei. The expression profiles using quantitative real-time polymerase chain reaction showed that TaGID2s were downregulated by GA3. The interaction between TaGID2s and TSK1 (homologous to ASK1) in yeast indicated that TaGID2s might function as a component of an E3 ubiquitin-ligase SCF complex. Yeast two-hybrid assays showed that a GA-independent interaction occurred between three TaGID2s and RHT-A1a, RHT-B1a, and RHT-D1a. Furthermore, TaGID2s interact with most RHT-1s, such as RHT-B1h, RHT-B1i, RHT-D1e, RHT-D1f, etc., but cannot interact with RHT-B1b or RHT-B1e, which have a stop codon in the DELLA motif, resulting in a lack of a GRAS domain. In addition, RHT-B1k has a frame-shift mutation in the VHIID motif leading to loss of the LHRII motif in the GRAS domain and RHT-D1h has a missense mutation in the LHRII motif. These results indicate that TaGID2s, novel positive regulators of the GA response, recognize RHT-1s in the LHRII motif resulting in poly-ubiquitination and degradation of the DELLA protein.
Thermal fluid dynamics of liquid bridge transfer in laser wire deposition 3D printing

Liquid bridge transfer mode is most favourable deposition pattern in laser wire deposition. However, the thermal fluid dynamics has not been well understood. In this paper, we systematically investigated the fluid dynamics during liquid bridge transfer in the printing process. We developed a novel three-dimensional heat transfer and fluid flow model by considering the effect of wire feeding. The results showed that for typical process parameters the Weber number (We) of the fluid on the liquid bridge is on the order of O(100∼101). A dimensionless slenderness number (Sl), was roughly estimated at the range of 3.17∼4.57 for maintaining the liquid bridge. This study provides the fluid mechanics insights of the metal transfer mechanisms in 3D printing process.
Life history and population dynamics of the surf clam, Mactra veneriformis (Bivalvia: Mactridae), on an estuarine intertidal sandflat in western Kyushu, Japan

Mactra veneriformis occurs commonly on estuarine tidal flats in temperate East Asia. Based on population sampling and recording of environmental variables over an extensive sandflat at a river mouth in Ariake Sound, southern Japan for 39 months, several reproductive traits were monitored and the recruitment, growth, and survivorship for cohorts tracked. The reproductive season ranged from May to September. Each year three to five newly-recruited cohorts appeared in June to October. A single cohort derived from adult spawning in late September occurred in December to January but disappeared by mid-May. Earlier summer recruits reached a maturation size in late September. Each year two or three cohorts survived until next February and fused into a composite cohort. High mortality in juveniles sometimes happened in the rainy period from June to July and at typhoon arrivals in July to October, with increased river discharges. Occasionally juvenile mass mortalities were observed following large wind-induced waves associated with typhoons. Despite their high mortality in the rainy period in some years, the fused adult cohorts contributed solely to the effective reproduction for the entire population. The surviving members of these cohorts died off during the following winter, with an estimated life span of 1.5 years.
The evolution of water balance in Glossina (Diptera: Glossinidae): correlations with climate

The water balance of tsetse flies (Diptera: Glossinidae) has significant implications for understanding biogeography and climate change responses in these African disease vectors. Although moisture is important for tsetse population dynamics, evolutionary responses of Glossina water balance to climate have been relatively poorly explored and earlier studies may have been confounded by several factors. Here, using a physiological and GIS climate database, we investigate potential interspecific relationships between traits of water balance and climate. We do so in conventional and phylogenetically independent approaches for both adults and pupae. Results showed that water loss rates (WLR) were significantly positively related to precipitation in pupae even after phylogenetic adjustment. Adults showed no physiology–climate correlations. Ancestral trait reconstruction suggests that a reduction in WLR and increased size probably evolved from an intermediate ancestral state and may have facilitated survival in xeric environments. The results of this study therefore suggest an important role for water balance physiology of pupae in determining interspecific variation and lend support to conclusions reached by early studies of tsetse physiology.
KEEFEKTIFAN MODEL PEMBELAJARANKOOPERATIF TIPE STAD DAN NHT TERHADAPKEMAMPUAN PEMECAHAN MASALAHMATEMATIKA PADA MATERI POKOK KUBUSDAN BALOK KELAS VIII SMPN 4 SEMARANG

Dalam melaksanakan proses kegiatan belajar mengajar, guru perlu terlebih dahulu merencanakan dengan seksama tentang strategi pembelajaran yang tepat, metode yang sesuai untuk pembelajaran suatu materi dan teknik bantu serta penggunaan teknologi informasi dan komunikasi seperti Lembar Kerja Siswa (LKS) yang dapat meningkatkan kemampuan pemecahan masalah peserta didik dalam proses belajar mengajar. Pengembangan model pembelajaran kooperatif tipe STAD dapat menjadi salah satu model pembelajaran yang dapat diterapkan dalam pembelajaran. Dalam pembelajaran ini, siswa akan belajar dalam suatu tim untuk menumbuhkan rasa percaya diri peserta didik untuk memecahkan permasalahan yang dianggap sulit. Rumusan masalah yang akan dikaji adalah bagaimana keefektifan model pembelajaran kooperatif tipe STAD dibandingkan dengan pembelajaran kooperatif tipe NHT terhadap kemampuan pemecahan masalah matematika pada materi pokok Kubus dan Balok peserta didik kelas VIII SMP N 4 Semarang? Populasi dalam penelitian ini adalah peserta didik kelas VIII semester 2 di SMP Negeri 4 Semarang dengan materi pokok Kubus dan Balok. Sampel dalam penelitian ini diambil dengan teknik cluster random sampling sebagai wakil dari populasi yang terbagi atas 2 kelompok. Variabel dalam penelitian ini adalah kemampuan pemecahan masalah matematika. Data penelitian ini diperoleh dari tes kemempuan pemecahan masalah peserta didik setelah memperoleh pembelajaran kooperatif tipe STAD dan NHT dan nilai ulangan harian materi Lingkaran, lembar observasi aktivitas peserta didik. Dalam pembelajaran keberhasilan yang ditetapkan adalah sekurang-kurangnya 80% peserta didik mendapat nilai ≥ 65. Berdasarkan perhitungan uji pada penelitian ini diperoleh z hitung = 1,861689 dan z tabel = 1,64 dengan α = 0,05. Karena z hitung > z tabel maka hasil tes kemampuan pemecahan masalah pada kelompok eksperimen I lebih baik dari kelas eksperimen II. Dengan demikian diperoleh simpulan bahwa model pembelajaran kooperatif tipe STAD lebih efektif daripada model pembelajaran kooperatif tipe NHT terhadap kemampuan pemecahan masalah matematika peserta didik kelas VIII SMP Negeri 4 Semarang materi pokok Kubus dan Balok. Oleh karena itu disarankan guru dapat memotivasi peserta didik untuk aktif sehingga tercipta suasana belajar yang baik antara peserta didik maupun guru dengan peserta didik. Pembelajaran kooperatif tipe STAD perlu terus dikembangkan dan diterapkan pada materi pokok yang lain.
Effects of spirituality in breast cancer survivors.

PURPOSE/OBJECTIVES To examine the effects of spirituality (meaning in life and prayer) on a sense of well-being among women who have had breast cancer. DESIGN Descriptive, correlational, cross-sectional. SETTING Rural and urban communities in central Texas. SAMPLE Convenience sample of 84 women, 34-80 years of age diagnosed less than one year previously (36%), within the previous one to five years (38%), and more than five years previously (26%). METHODS The study approach consisted of completing a questionnaire assessing personal and cancer characteristics, aspects of spirituality (meaning in life and prayer), and physical and psychological responses to breast cancer. MAIN RESEARCH VARIABLES Meaning in life, prayer, and physical and psychological responses to breast cancer. FINDINGS Meaning in life was positively related to psychological responses and negatively related to physical responses. Prayer was positively related to psychological well-being. Women with higher prayer scale scores reported lower education levels, less income to meet their needs, and closer relationships with God. Meaning in life mediated the impact of breast cancer on physical and psychological well-being. CONCLUSIONS Strong relationships exist among spirituality and personal and cancer characteristics. Meaning in life mediated the effects of breast cancer on well-being in breast cancer survivors. IMPLICATIONS FOR NURSING The findings support healthcare providers encouraging women diagnosed with breast cancer to explore their spirituality as an effective resource for dealing with the physical and psychological responses to cancer.
Properties of the electron cloud in a high-energy positron and electron storage ring

Low-energy, background electrons are ubiquitous in high-energy particle accelerators. Under certain conditions, interactions between this electron cloud and the high-energy beam can give rise to numerous effects that can seriously degrade the accelerator performance. These effects range from vacuum degradation to collective beam instabilities and emittance blowup. Although electron-cloud effects were first observed two decades ago in a few proton storage rings, they have in recent years been widely observed and intensely studied in positron and proton rings. Electron-cloud diagnostics developed at the Advanced Photon Source enabled for the first time detailed, direct characterization of the electron-cloud properties in a positron and electron storage ring. From in situ measurements of the electron flux and energy distribution at the vacuum chamber wall, electron-cloud production mechanisms and details of the beam-cloud interaction can be inferred. A significant longitudinal variation of the electron cloud is also observed, due primarily to geometrical details of the vacuum chamber. Furthermore, such experimental data can be used to provide realistic limits on key input parameters in modeling efforts, leading ultimately to greater confidence in predicting electron-cloud effects in future accelerators.
Active Complex Event Processing over Event Streams

State-of-the-art Complex Event Processing technology (CEP), while effective for pattern query execution, is limited in its capability of reacting to opportunities and risks detected by pattern queries. Especially reactions that affect the query results in turn have not been addressed in the literature. We propose to tackle these unsolved problems by embedding active rule support within the CEP engine, henceforth called Active CEP (ACEP). Active rules in ACEP allow us to specify a pattern query's dynamic condition and real-time actions. The technical challenge is to handle interactions between queries and reactions to queries in the high-volume stream execution. We hence introduce a novel stream-oriented transactional model along with a family of stream transaction scheduling algorithms that ensure the correctness of concurrent stream execution. We demonstrate the power of ACEP technology by applying it to the development of a healthcare system being deployed in UMass Medical School hospital. Through extensive performance experiments using real data streams, we show that our unique Low-Water-Mark stream transaction scheduler, customized for streaming environments, successfully achieves near-real-time system responsiveness and gives orders-of-magnitude better throughput than our alternative schedulers.
Using runs of homozygosity to detect genomic regions associated with susceptibility to infectious and metabolic diseases in dairy cows under intensive farming conditions

Runs of homozygosity (ROH) are contiguous stretches of homozygous genome which likely reflect transmission from common ances- tors and can be used to track the inheritance of haplotypes of interest. In the present paper, ROH were extracted from 50K SNPs and used to detect regions of the genome associated with susceptibility to diseases in a population of 468 Holstein-Frisian cows. Diagnosed diseases were categorised as infectious diseases, metabolic syndromes, mastitis, reproductive diseases and locomotive disorders. ROH associated with infectious diseases, mastitis and locomotive disorders were found on BTA 12. A long region of homozygosity linked with metabolic syndromes, infectious and reproductive diseases was detected on BTA 15, disclosing complex relationships between immunity, metabolism and functional disorders. ROH associated with infectious and reproductive diseases, mastitis and metabolic syndromes were observed on chromosomes 3, 5, 7, 13 and 18. Previous studies reported QTLs for milk production traits on all of these regions, thus substantiating the known negative relationship between selection for milk production and health in dairy cattle.
Toward Optimized Traffic Distribution for Efficient Network Capacity Utilization in Two-Layered Satellite Networks

A multi-layered satellite network (MLSN) appears to be a promising network for providing global ubiquitous broadband communication. To utilize the abundant network resources of the MLSNs, fair traffic distribution among its satellite layers is, indeed, important. In this paper, we propose a routing method to optimally distribute traffic load among the layers (i.e., the satellite layers in the MLSN). The load balancing scheme of the proposed routing method is developed by adopting a traffic distribution model, which is based upon network capacity estimation and theoretical analysis of the congestion rate in each layer. The performance of the proposed routing method has been validated through extensive computer simulations, which demonstrate that our traffic distribution model is reliable enough to characterize the traffic behavior in the MLSN. Furthermore, in contrast with the basic routing approach, our proposed routing method is more effective in terms of improved throughput and lower packet drops, which are optimized by the theoretical parameter setting.
Genome-wide association study identiﬁes multiple risk loci for renal cell carcinoma

Previous genome-wide association studies (GWAS) have identiﬁed six risk loci for renal cell carcinoma (RCC). We conducted a meta-analysis of two new scans of 5,198 cases and 7,331 controls together with four existing scans, totalling 10,784 cases and 20,406 controls of European ancestry. Twenty-four loci were tested in an additional 3,182 cases and 6,301 controls. We conﬁrm the six known RCC risk loci and identify seven new loci at 1p32.3 (rs4381241, P ¼ 3.1 (cid:2) 10 (cid:3) 10 ), 3p22.1 (rs67311347, P ¼ 2.5 (cid:2) 10 (cid:3) 8 ), 3q26.2 (rs10936602, P ¼ 8.8 (cid:2) 10 (cid:3) 9 ), 8p21.3 (rs2241261, P ¼ 5.8 (cid:2) 10 (cid:3) 9 ), 10q24.33-q25.1 (rs11813268, P ¼ 3.9 (cid:2) 10 (cid:3) 8 ), 11q22.3 (rs74911261, P ¼ 2.1 (cid:2) 10 (cid:3) 10 ) and 14q24.2 (rs4903064, P ¼ 2.2 (cid:2) 10 (cid:3) 24 ). Expression quantitative trait analyses suggest plausible candidate genes at these regions that may contribute to RCC susceptibility.
Dynamic Multisensory Integration: Somatosensory Speed Trumps Visual Accuracy during Feedback Control

Recent advances in movement neuroscience have consistently highlighted that the nervous system performs sophisticated feedback control over very short time scales (<100 ms for upper limb). These observations raise the important question of how the nervous system processes multiple sources of sensory feedback in such short time intervals, given that temporal delays across sensory systems such as vision and proprioception differ by tens of milliseconds. Here we show that during feedback control, healthy humans use dynamic estimates of hand motion that rely almost exclusively on limb afferent feedback even when visual information about limb motion is available. We demonstrate that such reliance on the fastest sensory signal during movement is compatible with dynamic Bayesian estimation. These results suggest that the nervous system considers not only sensory variances but also temporal delays to perform optimal multisensory integration and feedback control in real-time. SIGNIFICANCE STATEMENT Numerous studies have demonstrated that the nervous system combines redundant sensory signals according to their reliability. Although very powerful, this model does not consider how temporal delays may impact sensory reliability, which is an important issue for feedback control because different sensory systems are affected by different temporal delays. Here we show that the brain considers not only sensory variability but also temporal delays when integrating vision and proprioception following mechanical perturbations applied to the upper limb. Compatible with dynamic Bayesian estimation, our results unravel the importance of proprioception for feedback control as a consequence of the shorter temporal delays associated with this sensory modality.
A comparison of the antioxidant properties and total phenolic content in a Diatom, Chaetoceros sp. and a green microalga, Nannochloropsis sp.

The aquaculture industry commonly makes use of microalgae as live feed. Chaetoceros sp. , a diatom, and Nannochloropsis sp. , a unicellular green microalga, have both been reported to contain a substantial amount of polyunsaturated fatty acids and carotenoids. Their potential as natural sources of antioxidants has gained recent attention. This study focuses on determining the antioxidant properties of the different solvent extracts, namely hexane, dichloromethane, chloroform and methanol, from both microalgae. The evaluation of antioxidant capacities was done by Folin-ciocalteu, 1,1-diphenyl-2-picrylhydrazyl radical-scavenging, metal chelating, nitro-blue tetrazolium reduction and ferric-reducing antioxidant power assay. Results showed that the non-polar solvent extracts from the diatom were highest in antioxidant power, whereas both polar and non-polar solvent extracts of green microalgae showed good antioxidant potential. In general, Chaetoceros sp. had higher antioxidant capacities than Nannochloropsis sp. This study suggests that different solvent extracts contain different potential antioxidant compounds able to scavenge different types of free radicals.
Clustering Expressive Speech Styles in Audiobooks Using Glottal Source Parameters

A great challenge for text-to-speech synthesis is to produce ex- pressive speech. The main problem is that it is difficult to syn- thesise high-quality speech using expressive corpora. With the increasing interest in audiobook corpora for speech synthesis, there is a demand to synthesise speech which is rich in prosody, emotions and voice styles. In this work, Self-Organising Fea- ture Maps (SOFM) are used for clustering the speech data using voice quality parameters of the glottal source, in order to map out the variety of voice styles in the corpus. Subjective evalu- ation showed that this clustering method successfully separated the speech data into groups of utterances associated with dif- ferent voice characteristics. This work can be applied in unit- selection synthesis by selecting appropriate data sets to synthe- sise utterances with specific voice styles. It can also be used in parametric speech synthesis to model different voice styles separately.