Miracle-dz/UniFAR-FTUs huggingface dataset:
BACTERICIDAL TREATMENT OF HATCHING EGGS III: EFFECT OF ORGANIC CONTAMINANTS ON EFFICACY OF EGG SANITIZERS

SUMMARY The effectiveness of three sanitizers in killing Salmonella during room temperature storage with and without contaminating feces, egg, or chicken rinse for 48 h was tested. Uncontaminated sanitizers were tested, as were 50-mL solutions of 0.035% polyhexamethylenebiguanide hydrochloride (PHMB), 0.39% (1:256) Tektrol, and 1.4% H2O2, which were contaminated with 10 mL of a feces slurry, 10 mL of mixed egg, or 10 mL of chicken rinse. Each container was inoculated with approximately 1,000 cells of S. typhimurium and cultured after 1 and 5 min. After 24 and 48 h of storage, each original container was reinoculated and assayed for the new inoculum at 1 and 5 min postinoculation. Complete eradication of Salmonella was noted after 1 or 5 min exposure to fresh or stored uncontaminated sanitizer solutions. Tektrol became less active against Salmonella when contaminated with egg and stored for 24 h or more. However, feces or chicken rinse did not interfere with the activity of Tektrol. Hydrogen peroxide deteriorated most with storage with chicken feces and to a lesser extent chicken rinse. PHMB lost efficacy when exposed to chicken feces for 24 h or more, but was not affected by the other contaminants.
Oral Health and Menopause: A Comprehensive Review on Current Knowledge and Associated Dental Management

The menopause is physiological changes in women that give rise to adaptive changes at both systemic and oral level. As we all begin to reach an older age, dental health and hygiene becomes a major concern. The dentist is often the first person to appreciate numerous changes that are experienced throughout the body during menopause. The teeth and gums are extremely susceptible to any hormonal changes that take place just before menopause and readily decrease body's ability to fight off minor infections or maintain a healthy balance of useful and harmful bacteria within the oral environment. This review aimed to develop better understanding for major oro-dental complications observed in women during menopause, and schematic approach towards the different dental management protocols used during these periods. Various internets based popular search engines were used to explore related data from literature, which includes PubMed, PubMed Central, Cochrane, Google, Medknow, Ebsco, Science Direct, and IndMed. Upon compilation of relevant data, it was observed that periodontal health is most severely affected (up to 60%) followed by dry mouth (25%) and burning mouth (glossodynia; 15%) which, in turn, may increase the occurrence of oral mucosal and dental diseases, such as candidiasis. Though, the usage of hormone replacement therapy is effective but it does not necessarily prevent or help women with oral symptoms. Therefore, well controlled long-term randomized studies are needed to establish more authentic clinical guidelines for successful management of such conditions.
Psychological Factors Influencing the Effectiveness of Virtual Reality-Based Analgesia: A Systematic Review

The experience of pain is affected by remarkable psychological factors. The concept of neuromatrix suggests that pain is an amalgam of affect, cognition, and sensation mediated through diverse brain regions. Moreover, the experience of pain appears to be reduced by environmental stimuli that drive attention away from the noxious events. Accordingly, immersion in a computer-generated, three-dimensional virtual environment has been used as an efficient distraction tool in a number of studies on pain management. However, no systematic approaches have explored the psychological factors that influence the effectiveness of virtual reality (VR) as a distraction technology. This review aims to outline the fundamental psychological factors involved in the use of VR to provide pain management. An analysis of the literature revealed some important elements associated with the patients' subjective experience. Eleven studies met the inclusion criteria and were included in the review. The results suggest the importance of different psychological factors in the effectiveness of the analgesic distraction. While sense of presence influence the effectiveness of VR as a distraction tool, anxiety as well as positive emotions directly affect the experience of pain. Future challenges for pain management via VR include adopting properly validated measures to assess psychological factors and using different experimental conditions to better understand their complex effects.
Application of wMelPop Wolbachia Strain to Crash Local Populations of Aedes aegypti

The endosymbiotic bacteria Wolbachia pipientis (wMel strain) has been successfully established in several populations of Aedes aegypti, the primary dengue vector. The virulent Wolbachia strain wMelPop is known to cause several pathological impacts (increased egg mortality, life shortening, etc.) reducing overall fitness in the mosquito Ae. aegypti. Increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populations. We tested this application under semi-field cage conditions. First, we determined that wMelPop infection significantly reduced the survival of desiccation-resistant eggs of the dengue vector Ae. aegypti, with shade and temperature having a significant impact; nearly all wMelPop-infected eggs failed to hatch after 6 and 10 weeks in summer and winter conditions, respectively. In laboratory selection experiments we found that egg desiccation resistance can be increased by selection, and that this effect of wMelPop infection is due to the nuclear background of the host rather than Wolbachia. We then conducted an invasion of wMelPop within a semi-field cage using sustained weekly releases of wMelPop infected mosquitoes, with fixation achieved after 9 weeks. The egg populations wMelPop infected and an uninfected control were then subjected to a simulated prolonged monsoonal dry season (2.5 months) before flooding to induce hatching. The wMelPop infected eggs suffered significantly greater mortality than the controls, with only 0.67% and 4.35% of respective infected and uninfected eggs held in 99% shade hatching after 80 days. These studies suggest that wMelPop could be used to locally eliminate populations of Ae. aegypti that are exposed to prolonged dry conditions, particularly if combined with vector control.
Honey dressing versus paraffin tulle gras following toenail surgery.

OBJECTIVE Anecdotal reports suggest that certain honey dressings have a positive effect on wound healing. However, there is limited empirical evidence supporting its use. This double-blind randomised controlled trial investigated the effect of a honey dressing on wound healing following toenail surgery with matrix phenolisation. METHOD Participants (n=100) were randomly assigned to receive either an active manuka honey dressing (n=52) or paraffin-impregnated tulle gras (n=48). The primary outcome was time (days) taken for complete re-epithelialisation of the nail bed. RESULTS Mean healing times were 40.30 days (SD 18.21) for the honey group and 39.98 days (SD 25.42) for the paraffin tulle gras group. Partial avulsion wounds healed statistically significantly faster (p=0.01) with paraffin tulle gras (19.62 days, SD 9.31) than with the honey dressing (31.76 days, SD 18.8), but no significant difference (p=0.21) was found following total avulsion when comparing honey (45.28 days, SD 18.03.) with paraffin tulle gras dressings (52.03 days, SD 21.3). CONCLUSION The results suggest that patients may benefit more from paraffin tulle gras dressings than honey dressings following partial toenail avulsion. No statistically significant difference was found for healing times after total toenail avulsion, although the marginal benefit of the honey dressing on these healing times warrants further investigation.
Cooperative Local Caching Under Heterogeneous File Preferences

Local caching is an effective scheme for leveraging the memory of the mobile terminal (MT) and short range communications to save the bandwidth usage and reduce the download delay in the cellular communication system. In particular, the MTs first cache in their local memories in off-peak hours and then exchange the requested files with each other in the vicinity during peak hours. However, prior works largely overlook MTs’ heterogeneity in file preferences and their selfish behaviors. In this paper, we practically categorize the MTs into different interest groups according to the MTs’ preferences. Each group of MTs aims to increase the probability of successful file discovery from the neighboring MTs (from the same or different groups). Hence, we define the groups’ utilities as the probability of successfully discovering the file in the neighboring MTs, which should be maximized by deciding the caching strategies of different groups. By modeling MTs’ mobilities as homogeneous Poisson point processes, we analytically characterize MTs’ utilities in the closed form. We first consider the fully cooperative case where a centralizer helps all groups to make caching decisions. We formulate the problem as a weighted-sum utility maximization problem, through which the maximum utility tradeoffs of different groups are characterized. Next, we study two benchmark cases under selfish caching, namely, partial and no cooperation, with and without inter-group file sharing, respectively. The optimal caching distributions for these two cases are derived. Finally, numerical examples are presented to compare the utilities under different cases and show the effectiveness of the fully cooperative local caching compared with the two benchmark cases.
α2β1 integrin-mediated mechanical signals during osteodifferentiation of stem cells from the Wharton's jelly of the umbilical cord.

INTRODUCTION The formation and maintenance of tissues is regulated by various signals triggered by biological, chemical, and physical factors. Data increasingly confirm that matrix or tissue elasticity plays an influential role in regulating numerous cell functions. The aim of the present study was to better understand the regulation of cellular differentiation by mechanical cues. We studied the influence of matrix stiffness on the osteodifferentiation of two cell lineages characterized by different responses: mesenchymal stromal/stem cells isolated from the Wharton's jelly of the umbilical cord (UC-MSCs) with strong stiffness-dependent responses; and bone-derived cells (BDCs), which are insensitive to changes in matrix rigidity. The study also aimed to delineate how matrix stiffness affects intracellular signaling through focal adhesion kinase (FAK) activity—one of the key components in integrin-mediated signaling pathways. MATERIAL AND METHODS The effect of substrate stiffness on the expression of α2, α5, and β1 integrin was studied using real time PCR and Western blot using cells cultured in an osteogenic medium on tunable polyacrylamide gels coated with type I collagen, with elasticities corresponding to Young's moduli of 1.46 kPa and 26.12 kPa. FAK activity was monitored using ELISA assays. RESULTS We demonstrate for the first time the changes in the expression of α2, α5, and β1 integrin subunits in perinatal stem cells and in adult osteoblast precursor cells during in vitro osteogenic differentiation on surfaces characterized by different stiffness. We found that matrix rigidity significantly affects the osteogenic differentiation of UC-MSCs through α2 integrin-mediated mechanotransduction events, though not through the α5 integrin subunit. In BDCs, there were no significant changes in the expression levels of the tested protein associated with varying stiffness. CONCLUSIONS Our results provide evidence that matrix rigidity affects the osteogenic differentiation of UC-MSCs via mechanotransduction events mediated by α2 integrin subunits.
“Naked Microvascular Bone Flap” in Oral Reconstruction

AbstractSince the 1980s, bone free flaps have been used to reconstruct the maxilla and the mandible. The vascular pedicle, through the supply of nutritional substances and drugs from the bloodstream, ensures the vitality of the flap, rapid bone integration, and reduced risk of infection.However, due to many surgeons’ concerns about orocervical and orosinusal fistulas and infections, bone flaps are usually buried and protected by mucosal flaps or a second skin flap whenever it is not possible to harvest a skin paddle together with the bone flap.The authors, convinced that naked bone free flaps, if well vascularized, are capable of healing and repairing the osteomucosal deficit on their own, with no risk of infection or fistulas, began to harvest, for oral reconstructions, naked bone flaps, that is, bone flaps covered only by a muscle layer 5 to 20 mm thick.In this study, the authors present a review of their experience in oral cavity reconstructions by harvesting naked and covered bone free flaps, retrospectively evaluating the occurrence of major and minor, early and late complications, associated with the different reconstructive technique.
Steady‐state antigen‐expressing dendritic cells terminate CD4+ memory T‐cell responses

CD4+ T cells are important effectors of inflammation and tissue destruction in many diseases of immune dysregulation. As memory T cells develop early during the preclinical stages of autoimmune and inflammatory diseases, immunotherapeutic approaches to treatment of these diseases, once established, must include the means to terminate memory T‐cell responses. Traditionally, it has been considered that, due to their terminally differentiated nature, memory T cells are resistant to tolerance induction, although emerging evidence indicates that some immunotherapeutic approaches can terminate memory T‐cell responses. Here, we demonstrate that CD4+ memory T‐cell responses can be terminated when cognate antigen is transgenically expressed in steady‐state DC. Transfer of in‐vitro‐generated CD4+ memory T cells establishes, in nontransgenic recipients, a stable and readily recalled memory response to cognate antigen. In contrast, upon transfer to mice expressing cognate antigen targeted to DC, memory CD4+ T cells undergo a phase of limited proliferation followed by substantial deletion, and recall responses are effectively silenced. This finding is important in understanding how to effectively apply immunotherapy to ongoing T‐cell‐mediated autoimmune and inflammatory diseases.
Measuring author contributions to the Wikipedia

We consider the problem of measuring user contributions to versioned, collaborative bodies of information, such as wikis. Measuring the contributions of individual authors can be used to divide revenue, to recognize merit, to award status promotions, and to choose the order of authors when citing the content. In the context of the Wikipedia, previous works on author contribution estimation have focused on two criteria: the total text created, and the total number of edits performed. We show that neither of these criteria work well: both techniques are vulnerable to manipulation, and the total-text criterion fails to reward people who polish or re-arrange the content. We consider and compare various alternative criteria that take into account the quality of a contribution, in addition to the quantity, and we analyze how the criteria differ in the way they rank authors according to their contributions. As an outcome of this study, we propose to adopt total edit longevity as a measure of author contribution. Edit longevity is resistant to simple attacks, since edits are counted towards an author's contribution only if other authors accept the contribution. Edit longevity equally rewards people who create content, and people who rearrange or polish the content. Finally, edit longevity distinguishes the people who contribute little (who have contribution close to zero) from spammers or vandals, whose contribution quickly grows negative.
Trace: Tennessee Research and Creative Exchange Psychology Publications and Other Works Psychology Brain Reorganization as a Function of Walking Experience in 12-month-old Infants: Implications for the Development of Manual Laterality Recommended Citation Brain Reorganization as a Function of Walkin

reorganization as a function of walking experience in 12-month-old infants: implications for the development of manual laterality" (2014). Psychology Publications and Other Works. Hand preference in infancy is marked by many developmental shifts in hand use and arm coupling as infants reach for and manipulate objects. Research has linked these early shifts in hand use to the emergence of fundamental postural–locomotor milestones. Specifically, it was found that bimanual reaching declines when infants learn to sit; increases if infants begin to scoot in a sitting posture; declines when infants begin to crawl on hands and knees; and increases again when infants start walking upright. Why such pattern fluctuations during periods of postural–locomotor learning? One proposed hypothesis is that arm use practiced for the specific purpose of controlling posture and achieving locomotion transfers to reaching via brain functional reorganization. There has been scientific support for functional cortical reorganization and change in neural connectivity in response to motor practice in adults and animals, and as a function of crawling experience in human infants. In this research, we examined whether changes in neural connectivity also occurred as infants coupled their arms when learning to walk and whether such coupling mapped onto reaching laterality. Electroencephalogram (EEG) coherence data were collected from 43 12-month-old infants with varied levels of walking experience. EEG was recorded during quiet, attentive baseline. Walking proficiency was laboratory assessed and reaching responses were captured using small toys presented at mid-line while infants were sitting. Results revealed greater EEG coherence at homologous prefrontal/central scalp locations for the novice walkers compared to the prewalkers or more experienced walkers. In addition, reaching laterality was low in prewalkers and early walkers but high in experienced walkers. These results are consistent with the interpretation that arm coupling practiced during early walking transferred to reaching via brain functional reorganization, leading to the observed developmental changes in manual laterality.
Excision of malignant melanomas in North Wales: effect of location and surgeon on time to diagnosis and quality of excision.

BACKGROUND The epidemiology of melanoma is changing and its current management is variable, with some lesions being removed in general practice. We aimed to determine the quality of excision and time to diagnosis relating to the excising surgeon and the place of excision. METHOD Analysis of data from the North Wales Melanoma Database. RESULTS In total, 578 cases were diagnosed 1993-2001. There was a gender difference with anatomical location, with 107 (65%) males with lesions on their trunk compared to 57 (35%) females. Median Breslow thickness was 1.10 mm (range 0.05-16.0 mm). Ninety-five (16%) lesions were removed in general practice, of which 49 (52%) were referred on to hospital. In total, 266 (61%) lesions were excised with 'adequate' margins and 170 (39%) excised with margins narrower than the guidelines. General practice excisions were from a younger group than hospital excisions. There were no differences in quality of excision between general practice and hospital excisions. Time to diagnosis was shorter overall for general practice excisions than hospital excisions (median 12 versus 41 days, P < 0.001). CONCLUSION These findings are of policy importance in that there is no evidence from this study that general practice excisions are managed poorly or have a worse prognosis.
igh pre-freezing dilution improves post-thaw function of am spermatozoa

Despite considerable cryobiology research there is no industry standard for the concentration to which ram spermatozoa should be diluted before freezing. Ram semen is highly concentrated and often frozen at a high sperm concentration, necessitating the use of small laparoscopic insemination doses. The aim of this paper was to ascertain the effect of dilution on the integrity of frozen-thawed ram spermatozoa. In the first experiment, spermatozoa were extended with a Tris-buffered diluent before freezing or after thawing to yield a final sperm concentration of 20 × 106/ml, or were not diluted. Motility characteristics, viability and acrosome integrity of spermatozoa were analysed over a 6 h incubation period at 37 ◦C. In the second experiment, spermatozoa were either diluted before freezing, subjected to sex-sorting or not diluted before freezing. Thawed spermatozoa were separated into sub-populations using centrifugal counter-current distribution (CCCD) and the profile of partition and functional integrity (viability, chlortetracycline status and Annexin-V binding) in the sub-populations assessed. Dilution before freezing significantly improved post-thaw viability, acrosome integrity and total motility whereas dilution post-thaw decreased viability and motility of spermatozoa. Sperm heterogeneity, as assessed by CCCD profile, was not different for control, diluted and sex-sorted spermatozoa. Analysis of CCCD subpopulations showed the proportion of functional cells (displaying the F-Pattern or no PS translocation) was similar for all sperm types. The results show that ram spermatozoa retain normal function at higher pre-freeze dilution rates than are commonly used in the sheep industry. The application of these findings would result in more practicable and functional ion dos artificial inseminat
Role of carbohydrates in glycoprotein hormone signal transduction

The structure of the polypeptide chains and oligosaccharide moieties of the a and β subunits of pituitary and placental glycoprotein hormones are known. The dimeric polypeptide structure (but not the carbohydrate) is important for binding of the hormone to specific receptors. The N‐linked but not O‐linked carbohydrates, on the other hand, are required in some manner to activate the effector system. Hormones with depleted carbohydrate content (deglycosylated hormones) interact with receptor but are unable to activate intracellular events. Because of such discordant properties, these forms act as competitive inhibitors of hormone action. Through a combination of chemical deglycosylation procedures and site‐directed mutagenesis, the first site of N‐glycosylation from the NH2 terminus of the common α subunit has been identified to be most critical for glycoprotein hormone signal transduction. Control of glycosylation by the endocrine milieu could contribute to regulation of hormone function by secreting variable forms of agonist/antagonist. — Sairam, M. R. Role of carbohydrates in glycoprotein hormone signal transduction. FASEB J. 3: 1915‐1926; 1989.
Inverter nonlinearity effects in high-frequency signal-injection-based sensorless control methods

An analysis of pulsewidth-modulation inverter nonlinearities influencing high-frequency carrier-signal voltage injection for saliency-tracking-based rotor/flux position estimation is presented in this paper. Distortion of the injected carrier voltage caused by the nonlinear behavior of the inverter has been reported to cause errors in the estimated rotor/flux position. Though a number of techniques have been developed to compensate for inverter nonlinearities, they have not been proven to be effective when a high-frequency low-magnitude voltage needs to be generated. Both the origins of the distortion as well as the requirements for compensation methods to be effective when producing such high-frequency voltages will be established in this paper.