Miracle-dz/UniFAR-FTUs huggingface dataset:
Co-infection of methicillin-resistant Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus and extended spectrum β-lactamase producing Escherichia coli in bovine mastitis – three cases reported from India

Emergence of antimicrobial resistance among bovine mastitis pathogens is the major cause of frequent therapeutic failure and a cause of concern for veterinary practitioners. This study describes intra-mammary infection of methicillin-resistant Staphylococcus epidermidis (MRSE), methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum β-lactamase (ESBL) producing Escherichia coli in two Holstein Friesian crossbred cows with subclinical mastitis and one non-descript cow with clinical mastitis in two different districts of West Bengal, India. In total, three MRSE, one MRSA and three ESBL producing E. coli were isolated from these cases. Both the crossbreds were detected with MRSE (HFSE1 and HFSE2) and ESBL producing E. coli (HFEC1 and HFEC2), whereas, simultaneous infection of three pathogens viz. MRSA (NDSA1), MRSE (NDSE1) and ESBL producing E. coli (NDEC1) was found in the non-descript cow. The methicillin-resistant isolates possessed mecA gene and exhibited resistance to various antibiotics such as amikacin, tetracycline and glycopeptides. The ESBL producers were positive for blaCTX-M and blaTEM genes; in addition, HFEC1 and HFEC2 were positive for blaSHV and possessed the genes for class I integron (int1), sulphonamide resistance (sul1), quinolone resistance (qnrS) and other virulence factors (papC, iucD and ESTA1). All the ESBL producers exhibited resistance to a variety of antibiotics tested including third- and fourth-generation cephalosporins and were also intermediately resistant to carbapenems. This is the first ever report on simultaneous occurrence of MRSE, MRSA and ESBL producing E. coli in bovine mastitis indicating a major concern for dairy industry and public health as well.
Target prediction in Forward Scattering Radar

This paper describes aspects of ground target detection using a Forward Scattering Radar (FSR). The problem of extracting the Doppler signature in different interference environments is addressed. Hilbert Transform and Wavelet have been used to predict the existence of target. The paper begins with a brief description of the system, followed by a more detailed analytical study of predicting the presence of target in FSR. A practical experimentation has been realised to evaluate the proposed algorithm.
Purification and characterization of organellar DNA polymerases in the red alga Cyanidioschyzon merolae

DNA polymerase γ, a mitochondrial replication enzyme of yeasts and animals, is not present in photosynthetic eukaryotes. Recently, DNA polymerases with distant homology to bacterial DNA polymerase I were reported in rice, Arabidopsis, and tobacco, and they were localized to both plastids and mitochondria. We call them plant organellar DNA polymerases (POPs). However, POPs have never been purified in the native form from plant tissues. The unicellular thermotrophic red alga Cyanidioschyzon merolae contains two genes encoding proteins related to Escherichia coli DNA polymerase I (PolA and PolB). Phylogenetic analysis revealed that PolB is an ortholog of POPs. Nonphotosynthetic eukaryotes also have POPs, which suggested that POPs have an ancient origin before eukaryotic photosynthesis. PolA is a homolog of bacterial DNA polymerase I and is distinct from POPs. PolB was purified from the C. merolae cells by a series of column chromatography steps. Recombinant protein of PolA was also purified. Sensitivity to inhibitors of DNA synthesis was different in PolA, PolB, and E. coli DNA polymerase I. Immunoblot analysis and targeting studies with green fluorescent protein fusion proteins demonstrated that PolA was localized in the plastids, whereas PolB was present in both plastids and mitochondria. The expression of PolB was regulated by the cell cycle. The available results suggest that PolB is involved in the replication of plastids and mitochondria.
First Unions and the Stability of First Marriages.

The National Survey of Families and Households is used to examine the relationship between premarital cohabitation and marital instability among U.S. women born between 1928 and 1957. As previously observed cohabitation is generally associated with higher risks of marital dissolution. However that differential is much smaller (or reversed) in recent cohorts where cohabitation is more common. The association between cohabitation and marital dissolution observed in earlier cohorts may reflect the select nature of those who cohabited and may largely disappear as cohabitation becomes more common. (EXCERPT)
Elicitor-Induced Spruce Stress Lignin (Structural Similarity to Early Developmental Lignins)

Suspension cultures of Picea abies (L.) Karst released polymeric material into the culture medium when treated with an elicitor preparation from the spruce needle pathogen Rhizosphaera kalkhoffii. The presence of lignin (about 35%, w/w) was demonstrated by phloroglucinol/HCI reactivity and quantitation with thioglycolic acid. Carbohydrate (about 14%, w/w) and protein (about 32%, w/w) were also detected. Amino acid analysis revealed that hydroxyproline and proline predominated. Thioacidolysis and subsequent Raney nickel desulfurization allowed the analysis of lignin-building units and interunit bonds. Compared with spruce wood lignin, an approximately 20-fold higher relative amount of p-hydroxyphenyl units was determined. A high content of p-hydroxyphenyl units is typical for certain developmental lignins, such as conifer compression wood and middle lamella lignins, as well as all induced cell culture lignins so far analyzed. Cross-linkages of the pinoresinol type ([beta]-[beta]) in the excreted cell culture lignin were markedly increased, whereas [beta]-1 interunit linkages were decreased relative to spruce wood lignin. The amount and nature of cross-linkages were shown to be intermediate between those in wood lignin and in enzymatically prepared lignins. In summary, the elicitor-induced stress lignin was excreted as a lignin-extensin complex that closely resembled early developmental lignins.
Neurofibromin controls macropinocytosis and phagocytosis in Dictyostelium

Cells use phagocytosis and macropinocytosis to internalise bulk material, which in phagotrophic organisms supplies the nutrients necessary for growth. Wildtype Dictyostelium amoebae feed on bacteria, but for decades laboratory work has relied on axenic mutants that can also grow on liquid media. We used forward genetics to identify the causative gene underlying this phenotype. This gene encodes the RasGAP Neurofibromin (NF1). Loss of NF1 enables axenic growth by increasing fluid uptake. Mutants form outsized macropinosomes which are promoted by greater Ras and PI3K activity at sites of endocytosis. Relatedly, NF1 mutants can ingest larger-than-normal particles using phagocytosis. An NF1 reporter is recruited to nascent macropinosomes, suggesting that NF1 limits their size by locally inhibiting Ras signalling. Our results link NF1 with macropinocytosis and phagocytosis for the first time, and we propose that NF1 evolved in early phagotrophs to spatially modulate Ras activity, thereby constraining and shaping their feeding structures. DOI: http://dx.doi. org/10.7554/eLife.04940. 001
Psychological state and psychological strain in relation to infertility

The purpose of the study was to explore the relationship between psychological state and perceived strain in relation to fertility problems. Couples presenting at an infertility clinic completed, on two occasions seven months apart, a series of standardized measures to assess psychopathology. In addition, they completed a weekly diary rating their mood state or distress/strain over a period of 22 weeks, during which investigations and treatment were taking place. Little evidence of psychopathology was found in the sample; the weekly diaries also suggested a general lack of distress/strain, even in response to medical investigations and diagnosis. The results are discussed in the light of wide individual variations in ratings obtained from the diaries. Participants with high distress/strain scores tended to have significantly higher initial scores on the psychometric measures administered. Participants with high distress/strain scores were also more likely to show a further significant increase in distress/strain scores in relation to diagnostic information. It is argued that a task for future research is to determine the characteristics of those couples who are likely to experience difficulty in coping with the strain of infertility investigations so that counselling resources and support services can be most effectively targeted. © 1998 John Wiley & Sons, Ltd.
Use of Omeprazole as a CYP3A Probe Drug: Effect of Sex and Menstrual Cycle Phase on CYP3A Activity in Healthy Caucasian Adults

To determine the effects of sex and menstrual cycle phase on CYP3A activity and to characterize the intraindividual variability of CYP3A, 24 Caucasian adults were given a single dose of omeprazole every 14th day for 3 months (men) or during the mid–follicular and mid–luteal phases over 3 full menstrual cycles (women). The 2‐hour plasma metabolic ratio (MR) (omeprazole/omeprazole sulphone) was used as a measure of CYP3A activity. Overall mean MRs for men (2.4 ± 1.2) and women (2.3 ± 0.93) were not significantly different. In women, no difference in mean omeprazole MR was observed between the mid–follicular phase (2.2 ± 0.85) and mid–luteal phase (2.4 ± 1.0). When all data in the mid–luteal phase were stratified into 3 groups based on the progesterone concentrations (>50 nM [A], 20–50 nM [B], and <20 nM [C]), the MR of group A was significantly lower than that of B and C. The MRs of women during the mid–luteal phase were weakly correlated with progesterone concentrations (r = −0.35; P = .03). Individual coefficients of variation (CV%) in MR ranged from 8.7% to 60.2%, with a median 30.0%. No sex or menstrual cycle differences in CYP3A activity as measured by the probe drug omeprazole were seen. Higher CYP3A activity in women may be associated with higher endogenous progesterone concentrations.
Case Study: Psychopharmacogenetic Aspects of Prader-Willi Syndrome

Abstract The study of genes, drugs, and behavior in three male adolescents with Prader-Willi syndrome (PWS) revealed a clinical profile that raises questions about the indications for neuroleptic and appetite-suppressing medications in this condition. Evidence of the inadvisability of neuroleptic medication and of the pathophysiology of PWS has led to a remarkable control of violent outbursts and hyperphagia by carbamazepine in one patient afflicted with both PWS and Klinefelter's syndrome. Testosterone and behavioral therapy proved to be useful in the management of two patients. The present observations, which are supported by recent advances in the pathophysiology of satiety, suggest that PWS should be understood as a metabolic disorder and subjected to psychopharmaco-genetic study.
Yield Effects of Genetically Modified Crops in Developing Countries

Onfarm field trials carried out with Bacillus thuringiensis (Bt) cotton in different states of India show that the technology substantially reduces pest damage and increases yields. The yield gains are much higher than what has been reported for other countries where genetically modified crops were used mostly to replace and enhance chemical pest control. In many developing countries, small-scale farmers especially suffer big pest-related yield losses because of technical and economic constraints. Pest-resistant genetically modified crops can contribute to increased yields and agricultural growth in those situations, as the case of Bt cotton in India demonstrates.
The Role of the N-D1 Linker of the N-Ethylmaleimide-Sensitive Factor in the SNARE Disassembly

N-ethylmaleimide-sensitive factor (NSF) is a member of the type II AAA+ (ATPase associated with various cellular activities) family. It plays a critical role in intracellular membrane trafficking by disassembling soluble NSF attachment protein receptor (SNARE) complexes. Each NSF protomer consists of an N-terminal domain (N domain) followed by two AAA ATPase domains (D1 and D2) in tandem. The N domain is required for SNARE/α-SNAP binding and the D1 domain accounts for the majority of ATP hydrolysis. Little is known about the role of the N-D1 linker in the NSF function. This study presents detailed mutagenesis analyses of NSF N-D1 linker, dissecting its role in the SNARE disassembly, the SNARE/α-SNAP complex binding, the basal ATPase activity and the SNARE/α-SNAP stimulated ATPase activity. Our results show that the N-terminal region of the N-D1 linker associated mutants cause severe defect in SNARE complex disassembly, but little effects on the SNARE/α-SNAP complex binding, the basal and the SNARE/α-SNAP stimulated ATPase activity, suggesting this region may be involved in the motion transmission from D1 to N domain. Mutating the residues in middle and C-terminal region of the N-D1 linker increases the basal ATPase activity, indicating it may play a role in autoinhibiting NSF activity until it encounters SNARE/α-SNAP complex substrate. Moreover, mutations at the C-terminal sequence GIGG exhibit completely abolished or severely reduced activities of the substrate binding, suggesting that the flexibility of N-D1 linker is critical for the movement of the N domain that is required for the substrate binding. Taken together, these data suggest that the whole N-D1 linker is critical for the biological function of NSF to disassemble SNARE complex substrate with different regions responsible for different roles.
Betting on Hitler - the Value of Political Connections in Nazi Germany

This paper examines the value of connections between German industry and the Nazi movement in early 1933. Drawing on previously unused contemporary sources about management and supervisory board composition and stock returns, we find that one out of seven firms, and a large proportion of the biggest companies, had substantive links with the National Socialist German Workers’ Party. Firms supporting the Nazi movement experienced unusually high returns, outperforming unconnected ones by 5% to 8% between January and March 1933. These results are not driven by sectoral composition and are robust to alternative estimators and definitions of affiliation.
Managing Expert Knowledge: Organizational Challenges and Managerial Futures for the UK Medical Profession

The blurring of managerial and professional jurisdictions remains a significant area of organizational research. This process is often described as involving `re-stratification', the drawing of professional elites into bureaucratic roles; or `bureaucratization', the standardization of work operating procedures. We examine these processes further through considering how professional work is reordered through the application of knowledge management techniques, focusing in particular on the management of knowledge around clinical risk. We suggest attempts by hospital risk managers to manage medical knowledge towards organizational learning represent a significant challenge to clinical freedom, given the centrality of expert knowledge to professional autonomy. In considering this challenge, we are attentive to the idea that change occurs not through the top-down challenge of management, nor the bottom-up resistance of professionals, but through the dynamic mediation of these influences within a wider institutional context. Accordingly, we find that doctors respond to change through a number of situated responses that limit management control over knowledge and reinforce claims to medical autonomy. In extending professional jurisdiction for the management of knowledge, we show how professionals such as doctors can themselves become managerialized as they seek to stave off managerial encroachment. Rather than seeing professionals as being drawn into management roles or bureaucratic ways of working, we suggest that managerial techniques and jurisdictions are also strategically drawn into professional practice and identity.
STREET-LEVEL BUREAUCRACY AND PUBLIC ACCOUNTABILITY

The concept of ‘ street-level bureaucracy ’ was coined by Michael Lipsky (1980) as the common denominator for what would become a scholarly theme. Since then his stress on the relative autonomy of professionals has been complemented by the insight that they are working in a micro-network of relations, in varying contexts. The conception of ‘ governance ’ adds a particular aspect to this: the multi-dimensional character of a policy system as a nested sequence of decisions. Combining these views casts a different perspective on the ways street-level bureaucrats are held accountable. In this article some axiomatic assumptions are drawn from the existing literature on the theme of street-level bureaucracy and on the conception of governance. Acknowledging variety, and arguing for contextualized research, this results in a rethinking of the issue of accountability at the street level.
Phase-Amplitude Coupling and Long-Range Phase Synchronization Reveal Frontotemporal Interactions during Visual Working Memory

It has been suggested that cross-frequency phase-amplitude coupling (PAC), particularly in temporal brain structures, serves as a neural mechanism for coordinated working memory storage. In this magnetoencephalography study, we show that during visual working memory maintenance, temporal cortex regions, which exhibit enhanced PAC, interact with prefrontal cortex via enhanced low-frequency phase synchronization. Healthy human participants were engaged in a visual delayed match-to-sample task with pictures of natural objects. During the delay period, we observed increased spectral power of beta (20–28 Hz) and gamma (40–94 Hz) bands as well as decreased power of theta/alpha band (7–9 Hz) oscillations in visual sensory areas. Enhanced PAC between the phases of theta/alpha and the amplitudes of beta oscillations was found in the left inferior temporal cortex (IT), an area known to be involved in visual object memory. Furthermore, the IT was functionally connected to the prefrontal cortex by increased low-frequency phase synchronization within the theta/alpha band. Together, these results point to a mechanism in which the combination of PAC and long-range phase synchronization subserves enhanced large-scale brain communication. They suggest that distant brain regions might coordinate their activity in the low-frequency range to engage local stimulus-related processing in higher frequencies via the combination of long-range, within-frequency phase synchronization and local cross-frequency PAC. SIGNIFICANCE STATEMENT Working memory maintenance, like other cognitive functions, requires the coordinated engagement of brain areas in local and large-scale networks. However, the mechanisms by which spatially distributed brain regions share and combine information remain primarily unknown. We show that the combination of long-range, low-frequency phase synchronization and local cross-frequency phase-amplitude coupling might serve as a mechanism to coordinate memory processes across distant brain areas. In this study, low-frequency phase synchronization between prefrontal and temporal cortex co-occurred with local cross-frequency phase-amplitude coupling to higher frequencies in the latter. By such means, ongoing working memory storage taking place in higher frequencies in temporal regions might be effectively coordinated by distant frontal brain regions through synchronized activity in the low-frequency range.
Meta-Analysis of the Research on Response Cards

In this meta-analysis, the author analyzed 18 response card articles, theses, or dissertations to determine the magnitude of effect that response card strategies have on test achievement, quiz achievement, class participation, and intervals of off-task behavior. The author also determined whether the type of response cards used or the presence or absence of ceiling effects had a differential effect on study outcomes. Using the traditional method of hand raising as a control condition, he found that response cards have large, statistically significant effect sizes for test achievement, quiz achievement, participation, and reduction in intervals of disruptive behavior. No significant difference was found between types of response cards used. Although the difference was not statistically significant, studies with ceiling effects had, on average, effect sizes that were notably lower than studies without ceiling effects. Place of publication, type of publication, and sample size were not significant moderators of effect sizes for academic achievement.
The nature of nurture: Genetic influence on “environmental” measures

Abstract Evidence for genetic influence on environmental measures will emerge in quantitative genetic analyses if genetically influenced characteristics of individuals are assessed by these environmental measures. Recent twin and adoption studies indicate substantial genetic influence when measures of the environment are treated as phenotypes in genetic analyses. Genetic influence has been documented for measures as diverse as videotaped observations of parental behavior toward their children, ratings by parents and children of their family environment, and ratings of peer groups, social support, and life events. Evidence for genetic influence on environmental measures includes some of the most widely used measures of environment – the Home Observation for Measurement of the Environment, the Family Environment Scales, and the Social Readjustment Rating Scale of life events, for example. The goal of this article is to document and discuss these findings and to elicit commentary that might help to shape the course of research on this topic, which has far-reaching implications for the behavioral and brain sciences.
Impact of personality on the recognition of emotion expressed via human, virtual, and robotic embodiments

In this paper, we describe the elaboration and the validation of a body and face database1, of 96 videos of 1 to 2 seconds of duration, expressing 4 emotions (i.e., anger, happiness, fear, and sadness) elicited through 4 platforms of increased visual complexity and level of embodiment. The final aim of this database is to develop an individualized training program designed for individuals suffering of autism in order to help them recognize various emotions on different test platforms: two robots, a virtual agent, and a human. Before assessing the recognition capabilities of individuals with ASD, we validated our video database on typically developed individuals (TD). Moreover, we also looked at the relationship between the recognition rate and their personality traits (extroverted (EX) vs. introverted (IN)). We found that the personality of our TD participants did not lead to a different recognition behavior. However, introverted individuals better recognized emotions from less visually complex characters than extroverted individuals.