Miracle-dz/UniFAR-FTUs huggingface dataset:
In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. II. Inner cell masses from 3.5- to 4.0-day p.c. blastocysts.

This paper describes the development in culture of inner cell masses isolated immunosurgically from C3H/He mouse blastocysts immediately after collection between 3.5 and 4.0 days p.c. By 24--48 h most of the inner cell masses isolated from half-expanded blastocysts, and about 50% of those from expanded blastocysts, regenerate an outer layer of trophectoderm-like cells and so resemble mini-blastocysts. With further in vitro culture these structures attach to the substratum and give rise to trophoblast-like giant cells, together with clusters of parietal endoderm cells or inner cell masses surrounded by visceral endoderm. Many of the inner cell masses from the remaining expanded blastocysts develop into floating structures with an outer layer of endoderm cells, and by 7 days consist of a large fluid filled cyst surrounding a collapsed vesicle of epithelial cells. Mesodermal cells line the cysts and form numerous blood islands. When mechanically disrupted, and grown as attached sheets of cells, these cystic structures give rise to patches of trophoblast-like giant cells similar to those described in the previous paper. These results suggest that the inner cell mass of normal mouse blastocysts contains cells which are capable of giving rise to trophoblast in culture.
Phylogenetic expression of Galα1-4Gal on avian glycoproteins: Glycan differentiation inscribed in the early history of modern birds

Glycoproteins containing Galα1-4Gal (galabiose) had been rarely found in vertebrates, except in a few species of birds and amphibians. We had previously reported that pigeon (Columba livia) egg white and serum glycoproteins are rich in N-glycans with Galα1-4Gal at nonreducing termini. To investigate the origin of Galα1-4Gal expression in avian evolution, we examined the presence of Galα1-4Gal glycoproteins in egg whites from 20 orders, 88 families, 163 genera, and 181 species of birds, as probed by Western blot with Griffonia simplicifolia-I lectin (terminal α-Gal/GalNAc-specific) and anti-P1 mAb (Galα1-4Galβ1-4GlcNAcβ1-specific). One of the significant observations is the total absence of Galα1-4Gal glycoproteins in Struthioniformes (four species), Tinamiformes (three species), Craciformes (two species), Galliformes (14 species), and Anseriformes (10 species), which are phylogenetically separated from other orders at earlier stage of modern bird diversification (100-65 million years ago). The presence or absence of Galα1-4Gal glycoproteins in other avian orders varied by the species (104 species positive, and 44 species negative), even though some of them belong to the same order or family. Our results revealed that the expression of Galα1-4Gal glycoproteins is not rare among avians, and is correlated with the phylogeny. The expression was most likely differentiated at earlier stage of diversification in modern birds, but some birds might have lost the facility for the expression relatively recently.
Wellness, Work and Employee Assistance Programming

ABSTRACT Employee Assistance Programs continue to evolve and broaden the scope of programming provided to employees. Many EAPs now claim that Wellness programming is a core component. However, the definition of Wellness is often limited to only one or two dimensions of the concept. As well, EAPs generally have yet to fully examine the relationship between Wellness and the nature of work. This article's premise is that EAPs-should be working towards not only improving worker Wellness but also workplace Wellness.
An implementation of data encryption for Internet of Things using blowfish algorithm on FPGA

Information security has become an important issue in data communications. One method to ensure the security of data is to use cryptographic method. Cryptography is a method to encode the information to keep the information from being hacked by the other party. The implementation of cryptography is used a significant amount of computer resources. Various range application of blowfish algorithm can be implemented for data encryption sent from an Internet of Things physical network which have IP-based data. In this research, blowfish algorithm is implemented on FPGA using VHDL programming language, and monitored the number of FPGA resource that is used. The blowfish algorithm is analyzed by computing certain metrics performances such as security, encryption time, avalanche effect, and throughput from multiple testing scenarios for system reliability. The testing showed that blowfish algorithm gave a good performance when implemented in FPGA and show a good alternative to proposed as network security on Internet of Things.
Risk, Uncertainty and Entrepreneurship: Evidence from a Lab-in-The-Field Experiment

Theory predicts that entrepreneurs have distinct attitudes toward risk and uncertainty, but empirical evidence is mixed. To better understand the unique behavioral characteristics of entrepreneurs and the causes of these mixed results, we perform a large "lab-in-the-field" experiment comparing entrepreneurs to managers a suitable comparison group and employees n = 2,288. The results indicate that entrepreneurs perceive themselves as less risk averse than managers and employees, in line with common wisdom. However, when using experimental incentivized measures, the differences are subtler. Entrepreneurs are only found to be unique in their lower degree of loss aversion, and not in their risk or ambiguity aversion. This combination of results might be explained by our finding that perceived risk attitude is not only correlated to risk aversion but also to loss aversion. Overall, we therefore suggest using a broader definition of risk that captures this unique feature of entrepreneurs: their willingness to risk losses. This paper was accepted by Uri Gneezy, behavioral economics.
Problematic eating and feeding behaviors of 36-month-old children.

OBJECTIVE We incorporated selected items from several existing instruments to create a comprehensive multifactorial instrument to measure problematic eating behaviors in young children and to examine the prevalences and correlates of these behaviors. METHOD A community sample of young mothers (N = 93) completed the inventory of problematic eating behaviors for their 36-month-old children. RESULTS The most common child problems reported by mothers were the spitting out of food during feedings and becoming upset when food was restricted. A four-factor solution identified pickiness (e.g., child eats a limited variety of food), food refusal (e.g., child refuses to eat specific foods), struggle for control (e.g., frequent struggles with child over food), and positive parental behavior (e.g., praising child about his/her food intake) domains. Internal consistency was moderate to good for all factors. Only the struggle for control factor was related to other problematic behaviors as measured by the Child Behavior Check List (CBCL). The food refusal factor was related to mothers' lifetime history of any psychopathology. The pickiness factor was related to mothers' lifetime history of alcohol dependence. CONCLUSION Findings suggest that child clinicians should be sensitive to the quality of mother-child interactions during feeding.
Abstractive headline generation for spoken content by attentive recurrent neural networks with ASR error modeling

Headline generation for spoken content is important since spoken content is difficult to be shown on the screen and browsed by the user. It is a special type of abstractive summarization, for which the summaries are generated word by word from scratch without using any part of the original content. Many deep learning approaches for headline generation from text document have been proposed recently, all requiring huge quantities of training data, which is difficult for spoken document summarization. In this paper, we propose an ASR error modeling approach to learn the underlying structure of ASR error patterns and incorporate this model in an Attentive Recurrent Neural Network (ARNN) architecture. In this way, the model for abstractive headline generation for spoken content can be learned from abundant text data and the ASR data for some recognizers. Experiments showed very encouraging results and verified that the proposed ASR error model works well even when the input spoken content is recognized by a recognizer very different from the one the model learned from.
The Eolith Debate, Evolutionist Anthropology and the Oxford Connection Between 1880 and 1940

The eolith debate mirrors the development and demise of evolutionist anthropology in Britain between 1880 and 1940. This paper traces the connections between some of the key protagonists in the controversy, especially those associated with the Pitt-Rivers Museum at Oxford. The evolutionist pre-occupation of early Oxford anthropology with the continuity between archaeology and ethnology is shown to be linked to an interest in the Eolithic controversy, and these concerns persisted into a second generation as evolutionism was marginalized and prehistoric archaeology matured. Although the eolith debate finally floundered in the debris of the “epistemic rupture” between the world of Victorian evolutionism and late twentieth-century anthropology, some of its technical pre-occupations—particularly in relation to what we would now call ethnographic analogies and in terms of the techniques for distinguishing artefacts from geofacts—persist and are very much current issues.
Cobalamin (vitamin B12) and holotranscobalamin changes in plasma and liver tissue in alcoholics with liver disease.

OBJECTIVE We wanted to know if alterations in plasma cobalamin (B12) concentration and B12 carriers, e.g., holotranscobalamins (holo TC), occur in blood and liver tissue from patients with severe alcoholic liver disease. Our purpose was to test the hypothesis that liver disease may disrupt B12 distribution. METHOD Total B12, as well as B12 bound to transcobalamin I, II, III (holo TC), were measured to determine their concentration in plasma and in liver tissue; Poteriochromonas malhamensis--a protozoan reagent served to measure only metabolically active (true) B12. Total B12 as distributed in holo TC in plasma and liver tissue of healthy subjects (controls) were compared to patients with severe alcoholic liver disease. RESULTS Severe liver disease initiates highly elevated B12 levels in plasma and a lowered liver tissue total B12 concentration. The percent of B12 distributed to holo TC II is significantly depleted during liver disease. In contrast, holo TC I and III are elevated in plasma during liver disease and contain more B12 than controls. Total B12 and B12 distributed to TC are lower in diseased liver tissue. CONCLUSION Severe alcoholic liver disease involves leakage of total B12 from liver tissue into the plasma. Holo TC I and III concentration increases in plasma; this preserves the high plasma B12 from being excreted. However, plasma holo TC II B12 distribution is decreased, indicating that there is a depression of exogenous B12 entering the plasma and tissues. In severe liver disease, liver tissue B12 binding and storage by TC is disrupted and causes B12 to leak out of the liver into the circulation. Eventually liver disease could produce enough severe tissue B12 deficits to cause metabolic dysfunction despite elevated plasma total B12. Elevation of plasma B12, accompanied by a lowering of holo TC II distribution, seemed to be a useful index of liver disease severity suggesting preventive treatment.
Direct Activation of Bax by p53 Mediates Mitochondrial Membrane Permeabilization and Apoptosis

The tumor suppressor p53 exerts its anti-neoplastic activity primarily through the induction of apoptosis. We found that cytosolic localization of endogenous wild-type or trans-activation–deficient p53 was necessary and sufficient for apoptosis. p53 directly activated the proapoptotic Bcl-2protein Bax in the absence of other proteins to permeabilize mitochondria and engage the apoptotic program. p53 also released both proapoptotic multidomain proteins and BH3-only proteins [Proapoptotic Bcl-2family proteins that share only the third Bcl-2homology domain (BH3)] that were sequestered by Bcl-xL. The transcription-independent activation of Bax by p53 occurred with similar kinetics and concentrations to those produced by activated Bid. We propose that when p53 accumulates in the cytosol, it can function analogously to the BH3-only subset of proapoptotic Bcl-2proteins to activate Bax and trigger apoptosis.
High School Students and Their Lack of Preparedness for College: A Statewide Study

In this study, the authors examined the college-ready graduate rates of all students (n = 1,099 high schools) in the State of Texas for the 2006-2007 school year. Data were analyzed for students’ scores in reading, in math, and in both subject areas combined. Approximately one-third of all students were determined to be college-ready in both subject areas. Statistically significant and practically relevant differences, reflecting moderate to large effect sizes, were present in reading, math, and both subjects among Hispanic, African American, and White students. Concerns are expressed about the lack of preparedness of students for college and about the presence of strong achievement differences as a function of ethnicity. Implications of these findings are discussed.
ALADINI482S causes selective failure of nuclear protein import and hypersensitivity to oxidative stress in triple A syndrome

Triple A syndrome is an autosomal recessive neuroendocrinological disease caused by mutations in a gene that encodes 546 amino acid residues. The encoded protein is the nucleoporin ALADIN, a component of nuclear pore complex (NPC). We identified a mutant ALADINI482S that fails to target NPC and investigated the consequences of mistargeting using cultured fibroblasts (I482Sf) from a patient with triple A syndrome. ALADINI482S affected a karyopherin-α/β-mediated import pathway and decreased nuclear accumulations of aprataxin (APTX), a repair protein for DNA single-strand breaks (SSBs), and of DNA ligase I in I482Sf. This decrease was restored by wild-type ALADIN. ALADINI482S had no effect on imports of M9/kap-β2, BIB/kap-β3, histone H1/importin 7, the ubiquitin conjugating enzyme UbcM2/importin 11, or the spliceosome protein U1A, indicating that ALADINI482S selectively impaired transport of discrete import complexes through NPC. Cell survival assay showed hypersensitivity of I482Sf to l-buthionine-(S,R)-sulfoximine (BSO), a glutathione-depleting agent. BSO decreased nuclear APTX and ligase I levels in I482Sf and normal control fibroblasts, but increased SSBs only in I482Sf. These observations implied that I482Sf are hypersensitive to BSO and no longer sufficiently repair SSBs. Consistent with this notion, I482Sf transfected with both APTX and ligase I had increased resistance to BSO, whereas I482Sf transfected with LacZ vector remained hypersensitive to BSO. We propose that oxidative stress aggravates nuclear import failure, which is already compromised in patient cells. Consequent DNA damage, beyond the limited capacity of DNA repair proteins, i.e., APTX and ligase I, may participate in triggering cell death.
On the possibility of political change

We revisit the study of voting over education subsidies where poor individuals may be excluded and the rich may chose private alternatives. Introducing a new way of analyzing this type of problem we show that under realistic assumptions there are typically multiple equilibria. Shifts between these can only happen through "shocks to policy", not through gradual change. The reason for this is that even in the presence of a "Condorcet winner" there are typically also "local equilibria" which turn out to not only defeat neighboring policies but a broad range of alternatives. When introducing costs of changing policy these can become stable outcomes implying, for example, that identical countries or regions with di¤erent starting points could end up with completely di¤erent redistributive systems. Outcomes change in intuitive ways with the parameters and several insights with respect to the possibilities of political change seem general for problems of redistribution with excludability. Keywords: political economy, political equilibrium, voting, redistribution, education subsidies, local equilibrium, non-median voter equilibrium JEL classi cation: D7, H2 Institute for International Economic Studies, Stockholm University, SE-10691 Stockholm, Sweden, e-mail: conny.olovsson@iies.su. se. Financial support from the Tore Browaldh and Jan Wallander foundation is gratefully acknowledged. ySITE, Stockholm School of Economics, P.O. Box 6501, SE-113 83 Stockholm, Sweden, e-mail: jesper.roine@hhs.se. Financial support from the Tore Browaldh and Jan Wallander foundation is gratefully acknowledged.
Anti-obesity effects of galangin, a pancreatic lipase inhibitor in cafeteria diet fed female rats

Abstract Context: Alpinia galanga Willd (Zingiberaceae) (AG) is a rhizomatous herb widely cultivated in shady regions of Malaysia, India, Indochina and Indonesia. It is used in southern India as a domestic remedy for the treatment of rheumatoid arthritis, cough, asthma, obesity, diabetes, etc. It was reported to have anti-obesity, hypoglycemic, hypolipidemic and antioxidant properties. Objective: A flavonol glycoside, galangin, was isolated from AG rhizomes. Based on its in vitro pancreatic lipase inhibitory effect, the study was further aimed to clarify whether galangin prevented obesity induced in female rats by feeding cafeteria diet (CD) for 6 weeks. Materials and methods: The in vitro pancreatic lipase inhibitory effect of galangin was determined by measuring the release of oleic acid from triolein. For in vivo experiments, female albino rats were fed CD with or without 50 mg/kg galangin for 6 weeks. Body weight and food intake was measured at weekly intervals. On day 42, serum lipids levels were estimated and then the weight of liver and parametrial adipose tissue (PAT) was determined. The liver lipid peroxidation and triglyceride (TG) content was also estimated. Results: The IC50 value of galangin for pancreatic lipase was 48.20 mg/mL. Galangin produced inhibition of increased body weight, energy intake and PAT weight induced by CD. In addition, galangin produced a significant decrease in serum lipids, liver weight, lipid peroxidation and accumulation of hepatic TGs. Conclusion: Galangin present in AG rhizomes produces anti-obesity effects in CD-fed rats; this may be mediated through its pancreatic lipase inhibitory, hypolipidemic and antioxidant activities.
Supplemental Oxygen Compromises the Use of Pulse Oximetry for Detection of Apnea and Hypoventilation During Sedation in Simulated Pediatric Patients

OBJECTIVE. The goal was to assess the time to recognition of apnea in a simulated pediatric sedation scenario, with and without supplemental oxygen. METHODS. A pediatric human patient simulator mannequin was used to simulate apnea in a 6-year-old patient who received sedation for resetting of a fractured leg. Thirty pediatricians participating in a credentialing course for sedation were randomly assigned to 2 groups. Those in group 1 (N = 15) used supplemental oxygen, and those in group 2 (N = 15) did not use supplemental oxygen. A third group (N = 10), consisting of anesthesiology residents (postgraduate years 2 and 3 equivalent), performed the scenario with oxygen supplementation, to ensure validity and reliability of the simulation. The time interval from simulated apnea to bag-mask ventilation was recorded. Oxygen saturation and Paco2 values were recorded. All recorded variables and measurements were compared between the groups. RESULTS. The time interval for bag-mask ventilation to occur in group 1 (oxygen supplementation) was significantly longer than that in group 2 (without oxygen supplementation) (173 ± 130 and 83 ± 42 seconds, respectively). The time interval for bag-mask ventilation to occur was shorter in group 3 (anesthesiology residents) (24 ± 6 seconds). Paco2 reached a higher level in group 1 (75 ± 26 mmHg), compared with groups 2 and 3 (48 ± 10 and 42 ± 3 mmHg, respectively). There was no significant difference between the groups in oxygen saturation values at the time of clinical detection of apnea (93 ± 5%, 88 ± 5%, and 94 ± 7%, respectively). CONCLUSIONS. Hypoventilation and apnea are detected more quickly when patients undergoing sedation breathe only air. Supplemental oxygen not only does not prevent oxygen desaturation but also delays the recognition of apnea.
Ethnicity Effects on Sentence Outcomes in Large Urban Courts: Comparisons Among White, Black, and Hispanic Defendants*

Objective. This study examines Hispanic‐black‐white differences in sentences imposed on offenders appearing in state felony courts. Methods. The present study uses data collected by the State Court Processing Statistics (SCPS) program of the Bureau of Justice Statistics for the years 1990, 1992, 1994, and 1996. Results. Hispanic defendants are sentenced more similarly to black defendants than white defendants. Both black and Hispanic defendants tend to receive harsher sentences than white defendants. Also, ethnicity effects are the largest in the sentencing of drug offenders, whereas race effects are largest in the sentencing of property offenders. Furthermore, the present study demonstrates that the failure to consider defendants' ethnicity in comparing black‐white sentence outcomes is likely to result in findings that misrepresent black‐white differences. Conclusions. The results clearly demonstrate the necessity of considering not only defendants' race (i.e., black‐white differences) in sentencing but expanding our focus to also include defendants' ethnicity (i.e., Hispanic‐white and Hispanic‐black differences).
Phorbol diester 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) up‐regulates the expression of estrogen receptors in human THP‐1 leukemia cells

In the present work, we have inspected expression of estrogen receptors (ER) and their regulation by the phorbol diester 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) in a leukemic cell line, the THP‐1 cells, using multiple experimental approaches. Firstly, ligand binding assay (LBA) revealed that control (unstimulated) THP‐1 cells express type I (high affinity, limited capacity) ER in the nuclear fraction only, whilst treatment of cells with TPA resulted in the appearance of type I ER in the soluble fraction as well, with the 50 ng/ml dose and the 48 h incubation time being the most effective experimental condition. A concomitant increase of type II ER was also seen in both soluble and nuclear cell fractions. Unstimulated THP‐1 cells were found to be ER negative by immunocytochemistry; conversely, cells exposed to 50 ng/ml TPA for 48 h stained positively for ER, with the majority of cells having a strong nuclear staining. Scrutiny of ER mRNA expression using reverse transcriptase‐polymerase chain reaction showed the presence of a wild type ER transcript in both control and TPA‐treated THP‐1 cells, though levels of ER mRNA were found to be comparatively higher in the latter. This combined evidence would imply that the TPA‐induced differentiation of THP‐1 cells is accompanied by the rise of high affinity (type I) ER, suggesting that estrogens may play a role in the regulation of macrophage activity during the inflammatory and/or the immune response. J. Cell. Biochem. 83: 390–400, 2001. © 2001 Wiley‐Liss, Inc.