Miracle-dz/UniFAR-FTUs huggingface dataset:
Reproducibility of subfoveal choroidal thickness measurements with enhanced depth imaging by spectral-domain optical coherence tomography.

PURPOSE To measure the interobserver reproducibility and intra-observer reproducibility of subfoveal choroidal thickness measurements performed by enhanced depth imaging of spectral-domain optical coherence tomography (EDI-OCT) in a population-based setting. METHODS The Beijing Eye Study 2011 was a population-based study performed in rural and urban regions of Greater Beijing. The study included 3468 individuals with a mean age of 64.6 ± 9.8 years (range, 50-93 years). The participants underwent EDI-OCT and the subfoveal choroidal thickness (SFCT) was measured. To examine the interobserver variability, all images were assessed by two examiners independently of each other within 2 months. To examine the intra-observer reproducibility, a smaller study sample consisting of 21 eyes of 21 healthy subjects from the Tongren Eye Center was included in the study. These latter subjects were scanned 10 times with 1 minute breaks between each examination. The SFCT was measured by the same observer within 2 weeks. The intrasession within subject SD, the coefficient of variation, and the intraclass correlation coefficient (ICC) were calculated. RESULTS EDI-OCTs were performed for 3233 subjects. Mean SFCT measured by grader one and grader two were 254.6 ± 107.3 μm and 253.8 ± 107.4 μm, respectively, with a mean difference of 3.14 ± 13.1 μm (95% confidence interval, 0.0, 24.0). Bland-Altman plot showed 1.9% (61/3233) points outside the 95% limits of agreement. For the assessment of the intra-observer reproducibility, the ICC was 1.00 (P < 0.001, and the mean coefficient of variation was 0.85% ± 1.48%). CONCLUSIONS Under routine examination conditions, SFCT measurements by EDI-OCT showed a high intra-observer reproducibility and interobserver reproducibility.
Hypertension and Economic Activities in Sao Paulo, Brazil

SUMMARY A study of the prevalence of hypertension was undertaken among workers in 10 subsectors of the economy in Sao Paulo, a major urban-industrial area of Brazil. Included in the study were 5500 subjects 15-65 years of age, employed in 57 randomly selected firms. Hypertension rates (DBP $ 90 mm Hg) were higher among males up to 44 years of age. There was a decreasing gradient from mild to moderate and severe forms in all groups. Severity tended to increase with age in all groups. Black males showed higher rates than whites (29.2% vs 16.7%, p < 0.05), the excess being partially accounted for by moderate and severe forms (40% vs 20%). Subjects who overworked showed a trend toward higher hypertension rates. Higher rates in four subsectors (metallurgy, finance, transport, and journalism), aside from the distribution of known risk factors and job selection, may reflect a variety of work-related stressors. (Hypertension 3 (suppl II): II-233-II-237, 1981)
Continuous-Wave Stimulated Emission Depletion Microscope for Imaging Actin Cytoskeleton in Fixed and Live Cells

Stimulated emission depletion (STED) microscopy provides a new opportunity to study fine sub-cellular structures and highly dynamic cellular processes, which are challenging to observe using conventional optical microscopy. Using actin as an example, we explored the feasibility of using a continuous wave (CW)-STED microscope to study the fine structure and dynamics in fixed and live cells. Actin plays an important role in cellular processes, whose functioning involves dynamic formation and reorganization of fine structures of actin filaments. Frequently used confocal fluorescence and STED microscopy dyes were employed to image fixed PC-12 cells (dyed with phalloidin- fluorescein isothiocyante) and live rat chondrosarcoma cells (RCS) transfected with actin-green fluorescent protein (GFP). Compared to conventional confocal fluorescence microscopy, CW-STED microscopy shows improved spatial resolution in both fixed and live cells. We were able to monitor cell morphology changes continuously; however, the number of repetitive analyses were limited primarily by the dyes used in these experiments and could be improved with the use of dyes less susceptible to photobleaching. In conclusion, CW-STED may disclose new information for biological systems with a proper characteristic length scale. The challenges of using CW-STED microscopy to study cell structures are discussed.
Hidden in the Light: Occupational Norms among Crack-Using Street-Level Sex Workers

This article explores the “hypersexuality” hypothesis and, in particular, the phenomenon of sex-for-crack exchanges, by drawing on recent ethnographic research with women crack users engaged in street-level sex work in New York City. Viewing sex work as work, the study identifies the existence of a hitherto hidden set of occupational norms which cohere around the concept of discrimination as a central organizing principle in street-level prostitution. The article describes the ways in which established norms in relation to price, sex acts, clients, and bartering practices govern commercial sex transactions at the street level and examines their effects in regulating both individual and collective conduct. The analysis draws attention to the deficits of previous research and, specifically, the absence of context and the lack of attention to shared cultural practices and occupational norms which have made possible the erasure of agency from representations of these women's lives.
Robustness of classifiers: from adversarial to random noise

Several recent works have shown that state-of-the-art classifiers are vulnerable to worst-case (i.e., adversarial) perturbations of the datapoints. On the other hand, it has been empirically observed that these same classifiers are relatively robust to random noise. In this paper, we propose to study a \textit{semi-random} noise regime that generalizes both the random and worst-case noise regimes. We propose the first quantitative analysis of the robustness of nonlinear classifiers in this general noise regime. We establish precise theoretical bounds on the robustness of classifiers in this general regime, which depend on the curvature of the classifier's decision boundary. Our bounds confirm and quantify the empirical observations that classifiers satisfying curvature constraints are robust to random noise. Moreover, we quantify the robustness of classifiers in terms of the subspace dimension in the semi-random noise regime, and show that our bounds remarkably interpolate between the worst-case and random noise regimes. We perform experiments and show that the derived bounds provide very accurate estimates when applied to various state-of-the-art deep neural networks and datasets. This result suggests bounds on the curvature of the classifiers' decision boundaries that we support experimentally, and more generally offers important insights onto the geometry of high dimensional classification problems.
Engaging in community conversation: A means to improving the paramedicine student clinical placement experience

Community conversations are structured, inclusive conversations that bring together a group of people to engage in meaningful conversation, share knowledge and ideas, and discuss solutions to complex problems. This article focuses on the methodological aspects of using community conversations as a research approach. Participants included paramedics, ambulance service managers, paramedicine students and paramedicine educators, who gathered at La Trobe University in Bendigo in February 2013 to attend a conference titled ‘Paramedic Education and Leadership. Conversations took place over three days, with participants spending around five hours discussing the key issues related to paramedicine student clinical placements. Three stages of community conversations are described, along with the participants’ evaluation of the process, leading to the conclusion that community conversations are a valuable and effective way to bring together groups of people to discuss ideas and solutions to complex social problems.
Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment

The antibacterial activity of recombinant human lactoferrin from rice (rhLF) compared with that of human milk lactoferrin (hLF) was evaluated against Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes. The hydrolysates of rhLF and hLF were found to be more active than native proteins against E. coli O157:H7, and their activity was independent of their iron saturation. The effect of different heat treatments on the antibacterial activity of apo-rhLF was studied and compared with hLF. We observed that an HTST pasteurization treatment did not affect the antimicrobial activity of lactoferrin against the pathogens studied. Furthermore, the activity of apo-rhLF and hLF against E. coli O157:H7 and L. monocytogenes in UHT milk and whey was assayed, finding a decrease in the number of bacteria, although lower than that observed in a broth medium. This study shows the similar antibacterial activity of rhLF and hLF which is important in order to consider the addition of rhLF as a supplement in special products.
THE HYDROLOGIC CYCLE AND THE WISDOM OF THE CHILD

It was neither theology nor psychology that drew us to ask small children for their perceptions and resource appraisals, but curiosity about the world inside peoples' heads and its relationship to the world perceived by science or, in this case, by adults. In pursuing such an inquiry, we seem to be unique. A review of the voluminous psychological literature from 1927 to the present finds that most studies of children's thoughts, concepts, and perceptions do not deal with children and the physical environment as they experience it.3 Elm Park Center, where our observations, interviews, and field trips took place, is a small day care center attached to an equally small training institution for day care workers. The twenty-four children of working parents who come each day are free to choose activities and to move indoors and outdoors in an open-education setting. They are a cross-section of their age group in Worcester, an industrial city of 176,000 in central Massachusetts. It is the special quality of preschool age, the mixture of innocence and worldliness, unspoiled by formal teaching, that allows us to report introductory findings derived with pleasure and delight. One of us, a resident in this day care setting, began a year ago to collect from the staff anecdotal material and observations related to the children's experiences with water. Our initial reaction as geographers, specialists in water resources and not in child development, was surprise at the sophistication of experience. For example, * We wish to thank Hilary L. Renwick for her creativity and skill in drawing illustrations more delightful than we were able to imagine.
Two missense mutations identified in venous thrombosis patients impair the inhibitory function of the protein Z dependent protease inhibitor

Summary Protein Z-dependent protease inhibitor (ZPI) is a plasma inhibitor of factor (F)Xa and FXIa. In an earlier study, five mutations were identified within the ZPI gene of venous thrombosis patients and healthy controls. Two of these were nonsense mutations and three were missense mutations in important regions of the protein. Here we report that two of these latter three mutations, F145L and Q384R, impair the inhibitory function of ZPI in vitro. Recombinant wild-type and mutant proteins were prepared; stability in response to thermal challenge was similar. Inhibition of FXa in the presence of the cofactor protein Z was reduced 68-fold by the Q384R mutant; inhibition of FXIa by the F145L mutant was reduced two- to three-fold compared to the wild-type ZPI. An analysis of all five ZPI mutations was undertaken in a cohort of venous thrombosis patients (n=550) compared to healthy controls (n=600). Overall, there was a modest increase in incidence of these mutations in the thrombosis group (odds ratio 2.0, 1.05–3.7, p=0.044). However, in contrast to W324X (nonsense mutation), the Q384R missense mutation and R88X nonsense mutation were evenly distributed in patients and controls; F145L was rare. The final mutation (S143Y) was also rare and did not significantly alter ZPI function in laboratory studies. The F145L and particularly the Q384R mutation impaired the function of the coagulation inhibitor ZPI; however, there was no convincing association between these mutations and venous thrombosis risk. The functional role for ZPI in vivo has yet to be clarified.
Cyclic Strain–Mediated Regulation of Vascular Endothelial Occludin and ZO-1: Influence on Intercellular Tight Junction Assembly and Function

Objective—The vascular endothelium constitutes a highly effective fluid/solute barrier through the regulated apposition of intercellular tight junction complexes. Because endothelium-mediated functions and pathology are driven by hemodynamic forces (cyclic strain and shear stress), we hypothesized a dynamic regulatory link between endothelial tight junction assembly/function and hemodynamic stimuli. We, therefore, examined the effects of cyclic strain on the expression, modification, and function of 2 pivotal endothelial tight junction components, occludin and ZO-1. Methods and Results—For these studies, bovine aortic endothelial cells were subjected to physiological levels of equibiaxial cyclic strain (5% strain, 60 cycles/min, 24 hours). In response to strain, both occludin and ZO-1 protein expression increased by 2.3±0.1-fold and 2.0±0.3-fold, respectively, concomitant with a strain-dependent increase in occludin (but not ZO-1) mRNA levels. These changes were accompanied by reduced occludin tyrosine phosphorylation (75.7±8%) and increased ZO-1 serine/threonine phosphorylation (51.7±9% and 82.7±25%, respectively), modifications that could be completely blocked with tyrosine phosphatase and protein kinase C inhibitors (dephostatin and rottlerin, respectively). In addition, there was a significant strain-dependent increase in endothelial occludin/ZO-1 association (2.0±0.1-fold) in parallel with increased localization of both occludin and ZO-1 to the cell–cell border. These events could be completely blocked by dephostatin and rottlerin, and they correlated with a strain-dependent reduction in transendothelial permeability to FITC-dextran. Conclusions—Overall, these findings indicate that cyclic strain modulates both the expression and phosphorylation state of occludin and ZO-1 in vascular endothelial cells, with putative consequences for endothelial tight junction assembly and barrier integrity.
SERUM GAMMA-GLUTAMYLTRANSFERASE AS A PROGNOSTIC INDICATOR OF NEONATAL VIABILITY IN NONDOMESTIC RUMINANTS

Abstract Rapid assessment of immune status in neonatal ruminants of endangered species facilitates early intervention in cases of inadequate passive transfer of maternal immunoglobulins. Serum gamma-glutamyltransferase (GGT) was used to evaluate suspected passive transfer status in 25 North Indian muntjac (Muntiacus muntjak vaginalis), 45 Cretan goats (Capra algagrus cretica), 20 white-lipped deer (Cervus albirostris), 25 Mhorr gazelles (Gazella dama mhorr), and 31 Soemmerring's gazelles (Gazella soemmerringi soemmerringi). Serum GGT, measured within 48 hr of birth, was compared with clinical condition at 5 days of age. Neonatal Soemmerring's and Mhorr gazelles with GGT >600 U/L were likely to survive without medical intervention, whereas GGT <400 U/L was a good indicator that the gazelle neonate would need medical intervention. Neonatal muntjac with GGT >200 U/L were also likely to survive without medical intervention. Because there is no gold standard for evaluating passive transfer status in neonatal nondomestic ruminants, it is recommended to evaluate the results of more than one diagnostic test, as well as clinical condition, in considering health status and disposition of neonatal ruminants of endangered species.
The categorization of expatriates and the support offered by host country nationals

Purpose - – Although several authors have suggested that host country nationals (HCNs) play an important role in the management of expatriates (e.g. Toh and DeNisi, 2003; Farh Design/methodology/approach - – The paper employs a dyadic survey approach to determine the antecedents to expatriate categorization and HCN socialization behaviors from the perspective of both the expatriate and HCN. Findings - – The results of survey data from 65 expatriate-HCN dyads indicated that expatriate ethnocentrism and the salience of the expatriates’ nationality were important predictors of categorization, but that categorization was related to only one dimension of socialization. However, affect was found to play a role in predicting socialization behaviors. Research limitations/implications - – There is potential selection bias since expatriates chose HCNs as respondents, but results suggested this was not a serious problem. Other limitations include a relatively small sample size and the fact that a number of contextual issues such as national stereotypes and MNC strategy, are not controlled for. Practical implications - – Implications of these findings for the successful management of expatriate assignments include sending over expatriates with the right relational skills, and those low in ethnocentrism, rather than just the right technical skills. Originality/value - – The present study was one of the first to empirically test the potential role of categorization in the process of socialization.
Income inequality and health: multilevel analysis of Chilean communities

Study objective: The evidence supporting the effect of income inequality on health has been largely observed in societies far more egalitarian than the US. This study examines the cross sectional multilevel associations between income inequality and self rated poor health in Chile; a society more unequal than the US. Design: A multilevel statistical framework of 98 344 people nested within 61 978 households nested within 285 communities nested within 13 regions. Setting: The 2000 National Socioeconomic Characterization Survey (CASEN) data from Chile. Participants: Adults aged 18 and above. The outcome was a dichotomised self rated health (0 if very good, good or average; 1 if poor, or very poor). Individual level exposures included age, sex, ethnicity, marital status, education, employment status, type of health insurance, and household level exposures include income and residential setting (urban/rural). Community level exposures included the Gini coefficient and median income. Main results: Controlling for individual/household predictors, a significant gradient was observed between income and poor self rated health, with very poor most likely to report poor health (OR: 2.94) followed by poor (OR: 2.77), low (OR: 2.06), middle (OR: 1.73), high (OR: 1.38) as compared with the very high income earners. Controlling for household and community effects of income, a significant effect of community income inequality was observed (OR:1.22). Conclusions: Household income does not explain any of the between community differences; neither does it account for the effect of community income inequality on self rated health, with more unequal communities associated with a greater probability of reporting poor health.
Design or screening of drugs for the treatment of Chagas disease: what shows the most promise?

Introduction: Endemic in Latin America, Chagas disease is now becoming a serious global health problem, and yet has no financial viability for the pharmaceutical industry and remains incurable. In 2012, two antimycotic drugs inhibitors of fungal sterol 14α-demethylase (CYP51) – posaconazole and ravuconazole – entered clinical trials. Availability of the X-ray structure of the orthologous enzyme from the causative agent of the disease, protozoan parasite Trypanosoma cruzi, determined in complexes with posaconazole as well as with several experimental protozoa-specific CYP51 inhibitors opens an excellent opportunity to improve the situation. Areas covered: This article summarizes the information available in PubMed and Google on the outcomes of treatment of the chronic Chagas disease. It also outlines the major features of the T. cruzi CYP51 structure and the possible structure-based strategies for rational design of novel T. cruzi specific drugs. Expert opinion: There is no doubt that screenings for alternative drug-like molecules as well as mining the T. cruzi genome for novel drug targets are of great value and might eventually lead to groundbreaking discoveries. However, all newly identified molecules must proceed through the long, expensive and low-yielding drug optimization process, and all novel potential drug targets must be validated in terms of their essentiality and druggability. CYP51 is already a well-validated and highly successful target for clinical and agricultural antifungals. With minimal investments into the final stages of their development/trials, T. cruzi-specific CYP51 inhibitors can provide an immediate treatment for Chagas disease, either on their own or in combination with the currently available drugs.
Language Independent Authorship Attribution with Character Level N-Grams

We present a method for computer-assisted authorship attribution based on character-level n-gram language models. Our approach is based on simple information theoretic principles, and achieves improved performance across a variety of languages without requiring extensive pre-processing or feature selection. To demonstrate the effectiveness and language independence of our approach, we present experimental results on Greek, English, and Chinese data. We show that our approach achieves state of the art performance in each of these cases. In particular, we obtain a 18% accuracy improvement over the best published results for a Greek data set, while using a far simpler technique than previous investigations.
Children in poverty: resilience despite risk.

Two objectives provided the focus for the Conference on Community Violence and Children's Development that was jointly sponsored by the National Institute of Mental Health and the John D. and Catherine T. MacArthur Foundation. One was to examine the evidence for deficit behaviors that characterized children reared in poverty; the second was to identify the characteristics of children who sustained their competencies despite being reared in comparable environments. These dual objectives took this form: "What can we conclude from studies of children, their families, and environments about characteristics that predispose children to maladjustment following exposure to violence, and about characteristics that protect children from such adjustment problems following, or in the midst of, violence exposure? "