Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in Meat Quality Characteristics of the Sous-vide Cooked Chicken Breast during Refrigerated Storage

This study was performed to investigate the changes in meat quality characteristics of the sous vide cooked chicken breast during refrigerated storage at 4℃ for 14 d between before and after sous-vide cooking. Cooking loss and shear force were significantly increased, whereas expressible drip was significantly decreased along with reduction in the water holding capacity in both of two groups. Redness of meat juice was significantly (p<0.05) increased during storage, and considerably increased in the refrigerated samples after sous-vide cooked at the 7 to 10 d. The thiobarbituric acid reactive substances (TBARS) was significantly increased and was higher in the refrigerator stored chicken breast samples after sous-vide cooking. The volatile basic nitrogen (VBN) value was significantly increased in both groups, but the VBN value of the stored raw meat sample before sous-vide cooking was increased at an early storage, while the VBN value of the stored sample after sous-vide cooking was increased gradually in this study. Total viable counts and coliform counts were significantly decreased during storage, and coliforms were not detected after 7 d of storage in both groups. Salmonella spp. was not detected during the whole studied period. The outcome of this research can provide preliminary data that could be used to apply for further study of chicken breast using sous-vide cooking method that could be attractive to consumers.
Successful Establishment of Plasmids R1 and pMV158 in a New Host Requires the Relief of the Transcriptional Repression of Their Essential rep Genes

Although differing in size, encoded traits, host range, and replication mechanism, both narrow-host-range theta-type conjugative enterobacterial plasmid R1 and promiscuous rolling-circle-type mobilizable streptococcal plasmid pMV158 encode a transcriptional repressor protein, namely CopB in R1 and CopG in pMV158, involved in replication control. The gene encoding CopB or CopG is cotranscribed with a downstream gene that encodes the replication initiator Rep protein of the corresponding plasmid. However, whereas CopG is an auto-repressor that inhibits transcription of the entire copG-repB operon, CopB is expressed constitutively and represses a second, downstream promoter that directs transcription of repA. As a consequence of the distinct regulatory pathways implied by CopB and CopG, these repressor proteins play a different role in control of plasmid replication during the steady state: while CopB has an auxiliary role by keeping repressed the regulated promoter whenever the plasmid copy number is above a low threshold, CopG plays a primary role by acting coordinately with RNAII. Here, we have studied the role of the regulatory circuit mediated by these transcriptional repressors during the establishment of these two plasmids in a new host cell, and found that excess Cop repressor molecules in the recipient cell result in a severe decrease in the frequency and/or the velocity of appearance of transformant colonies for the cognate plasmid but not for unrelated plasmids. Using the pMV158 replicon as a model system, together with highly sensitive real-time qPCR and inverse PCR methods, we have also analyzed the effect of CopG on the kinetics of repopulation of the plasmid in Streptococcus pneumoniae. We show that, whereas in the absence of CopG pMV158 repopulation occurs mainly during the first 45 min following plasmid transfer, the presence of the transcriptional repressor in the recipient cell severely impairs the replicon repopulation and makes the plasmid replicate at approximately the same rate as the chromosome at any time after transformation, which results in maximal plasmid loss rate in the absence of selection. Overall, these findings indicate that unrepressed activity of the Cop-regulated promoter is crucial for the successful colonization of the recipient bacterial cells by the plasmid.
Metabolomic fingerprint of severe obesity is dynamically affected by bariatric surgery in a procedure-dependent manner.

BACKGROUND Obesity is associated with multiple diseases. Bariatric surgery is the most effective therapy for severe obesity that can reduce body weight and obesity-associated morbidity. The metabolic alterations associated with obesity and respective changes after bariatric surgery are incompletely understood. OBJECTIVE We comprehensively assessed metabolic alterations associated with severe obesity and distinct bariatric procedures. DESIGN In our longitudinal observational study, we applied a (1)H-nuclear magnetic resonance-based global, untargeted metabolomics strategy on human serum samples that were collected before and repeatedly ≤1 y after distinct bariatric procedures [i.e., a sleeve gastrectomy, proximal Roux-en Y gastric bypass (RYGB), and distal RYGB]. For comparison, we also analyzed serum samples from normal-weight and less-obese subjects who were matched for 1-y postoperative body mass index (BMI) values of the surgical groups. RESULTS We identified a metabolomic fingerprint in obese subjects that was clearly discriminated from that of normal-weight subjects. Furthermore, we showed that bariatric surgery (sleeve gastrectomy and proximal and distal RYGB) dynamically affected this fingerprint in a procedure-dependent manner, thereby establishing new fingerprints that could be discriminated from those of BMI-matched and normal-weight control subjects. Metabolites that largely contributed to the metabolomic fingerprints of severe obesity were aromatic and branched-chain amino acids (elevated), metabolites related to energy metabolism (pyruvate and citrate; elevated), and metabolites suggested to be derived from gut microbiota (formate, methanol, and isopropanol; all elevated). CONCLUSION Our data indicate that bariatric surgery, irrespective of the specific kind of procedure used, reverses most of the metabolic alterations associated with obesity and suggest profound changes in gut microbiome-host interactions after the surgery. This trial was registered at clinicaltrials.gov as NCT02480322.
Signature miRNAs in colorectal cancers were revealed using a bias reduction small RNA deep sequencing protocol

To explore the role of miRNAs in colorectal cancers (CRC), we have deep sequenced 48 pairs of frozen CRC samples, of which 44 pairs produced high quality sequencing data. By using a combined approach of our bias reduction small RNA (smRNA) deep sequencing protocol and Illumina small RNA TruSeq method for sample bar coding, we have obtained data from samples of relatively large size with bias reduced digital profile results. This novel approach allowed us to validate many previously published results using various techniques to profile miRNAs in CRC tissues or cell lines and to characterize ‘true’ miRNA signatures highly expressed in colon/rectum (CR) or CRC tissues. According to our results, miR-21, a miRNA that is up-regulated in CRC, and miR-143, a miRNA that is down-regulated in CRC, are the two miRNAs that dominated the miRNA population in CR tissues, and probably are also the most important miRNAs in CRCs. These two miRNAs, together with the other eight miRNAs, miR-148a, -194, -192, 200b, -200c, -10b, -26a, and -145, with descending expressing levels, constituted the top 10 highly expressed miRNAs in CR/CRC. Using TaqMan miRNA qPCR, we detected the relative expression of some of the CRC miRNAs in 10 CRC cell lines, validated their dysregulation under cancer condition, and provided possible explanation for their dysregulation, which could be caused by APC, KRAS, or TP53 mutations. We believe these results will provide a new direction in future miRNA-related CRC development studies, and application of miRNAs in CRC diagnosis/prognosis, and therapy.
Effect of squalane on mebendazole-loaded Compritol® nanoparticles

The objective of this study is to develop nanostructured lipid formulations of Compritol for the delivery of mebendazole. The formulations were prepared with Compritol 888 ATO, squalane, and Pluronic F68. Nine batches with different amounts of modifier, squalane, and drug were prepared. The formulations were characterized by evaluating particle size, morphology, and zeta potential. The thermal properties of the formulations were analyzed by differential scanning calorimetry (DSC). The encapsulation efficiency of each formulation and the drug release rates from each formulation were quantified by UPLC. The particles were spherical and had median particle sizes between 300 and 600 nm (50th percentile). A linear relationship was observed between Compritol/squalane composition and the melting point of the mixture. The DSC scans of the formulations revealed some recrystallization of the drug from the formulations, and the amount of recrystallization correlated with the amount of squalane in the formulation. Approximately, 70% efficiency of encapsulation was observed in the formulations with 30% (w/w) squalane, and these formulations also had faster dissolution rates compared to the other formulations. Overall, the formulations with 30% squalane are the preferred formulation for future testing.
Identification of the core temperature in a fractional order noisy environment for thermal feedback in nuclear reactors

In this paper, the core temperature of a 540 MWe Pressurized Heavy Water Reactor (PHWR) has been estimated from the measured temperature of the coolant using system identification techniques. The formulation is done considering fractional order (FO) dynamics for the sensor noise (i.e. 1/ƒα noise) and the conventional white noise in measurement. Performance study with different least square based linear estimators and nonlinear AutoRegressive eXogenous (ARX) and nonlinear Hammerstein-Wiener class of estimators for system identification has been done to show their relative merits to handle fractional order noise dynamics for estimating reactor core temperature.
Evidence of a Novel Source of Random Telegraph Signal in CMOS Image Sensors

This letter reports a new source of dark current random telegraph signal in CMOS image sensors due to meta-stable Shockley-Read-Hall generation mechanism at oxide interfaces. The role of oxide defects is discriminated thanks to the use of ionizing radiations. A dedicated RTS detection technique and several test conditions (radiation dose, temperature, integration time, photodiode bias) reveal the particularities of this novel source of RTS.
Three Yersinia enterocolitica AmpD Homologs Participate in the Multi-Step Regulation of Chromosomal Cephalosporinase, AmpC

In many gram negative bacilli, AmpD plays a key role in both cell well-recycling pathway and β-lactamase regulation, inactivation of the ampD causes the accumulation of 1,6-anhydromuropeptides, and results in the ampC overproduction. In Yersinia enterocolitica, the regulation of ampC expression may also rely on the ampR-ampC system, the role of AmpD in this species is still unknown. In this study, three AmpD homologs (AmpD1, AmpD2, and AmpD3) have been identified in complete sequence of strain Y. enterocolitica subsp. palearctica 105.5R(r). To understand the role of three AmpD homologs, several mutant strains were constructed and analyzed where a rare ampC regulation mechanism was observed: low-effective ampD2 and ampD3 cooperate with the high-effective ampD1 in the three levels regulation of ampC expression. Enterobacteriaceae was used to be supposed to regulate ampC expression by two steps, three steps regulation was only observed in Pseudomonas aeruginosa. In this study, we first reported that Enterobacteriaceae Y. enterocolitica can also possess a three steps stepwise regulation mechanism, regulating the ampC expression precisely.
Discovery of Online Shopping Patterns Across Websites

In the online world, customers can easily navigate to different online stores to make purchases. The products purchased on one site are often associated with product purchases on other sites e.g., a hotel reservation on one site and a car rental on another site. Whereas market basket analysis is often used to discover associations among products for brick-and-mortar stores, it is rarely applied in the online setting where consumers navigate among different online stores to buy products. We define online shopping patterns and develop two novel methods to perform market basket analysis across websites. While this research is motivated by online shopping applications, our contribution is mainly methodological. The two methods we develop in this paper can not only be used to identify various online shopping patterns across sites and products but can also be applied to settings where patterns exist across different dimensions. Experiments on both synthetic data and real online shopping data demonstrate the effectiveness of our methods.
State Feedback Stabilization for Boolean Control Networks

State feedback stabilization for Boolean control networks is investigated in this technical note. Based on the algebraic representation of logical dynamics in terms of the semi-tensor product of matrices, a necessary and sufficient condition is derived for the existence of a globally stabilizing state feedback controller, and a general control design approach is proposed when global stabilization is feasible via state feedback. Instead of designing the logical form of a stabilizing feedback law directly, we first construct its algebraic representation and then convert the algebraic representation back to the logical form. An example is worked out to illustrate the proposed design procedure.
Paradoxical enlargement of lymph nodes during therapy of central nervous system tuberculosis.

1It is well documented in lymph nodes, with some series quoting an incidence of 30%, whereas in the brain, PR incidence has been reported to occur at a lesser degree. In a recent publication of a small series of CNS tuberculoma, PR occurred in 16% of the patients. 1-4 In pulmonary TB, the paradoxical reaction is rarely observed, and it takes the form of respiratory distress syndrome. 5 The prolonged fever occasionally seen in an otherwise satisfactory response to anti-TB may be another manifestation of PR. 1 Other tissues or organs in the human body may be affected by PR, but this has not yet been documented. 1 All the reactions described involve the initial site of the disease manifestation. We report a case of TB meningitis with cerebral and spinal tuberculomas, in which the PR affected the lymph nodes and not the CNS. Our purpose is to document this unique reaction and discuss its significance and possible mechanism.
Approximation of Log-Likelihood Ratio for Wireless Channels Based on Taylor Series

A new approach for the approximation of the channel log-likelihood ratio (LLR) for wireless channels based on Taylor series is proposed. The approximation is applied to the uncorrelated flat Rayleigh fading channel with unknown channel side information at the receiver. It is shown that the proposed approximation greatly simplifies the calculation of channel LLRs, and yet provides results almost identical to those based on the exact calculation of channel LLRs. The results are obtained in the context of iterative decoding of low-density parity-check (LDPC) codes and include threshold calculations and error rate performance of finite-length codes. Compared to the existing approximations, the proposed method is either significantly less complex, or considerably more accurate.
International Association for Ecology Effects of Maternal Nutrition and Egg Provisioning on Parameters of Larval Hatch , Survival and Dispersal in the Gypsy Moth

North American gypsy moths disperse as newly hatched larvae on wind currents in a behavior called ballooning. Because ballooning occurs before neonates begin to feed, resources used in dispersal are limited to those carried over from the egg. We show that nutritional experience of the maternal parent can influence the tendency of offspring to disperse, and that resource provisioning of eggs by the maternal parent affects the duration of the window for dispersal. Offspring of females from defoliated sites had a lower tendency to balloon in a wind tunnel than larvae from females which had not experienced nutritional stress associated with host defoliation. The number of eggs in an egg mass, a reflection of the maternal parent's nutritional experience, also contributed to the predictive model for dispersal that included defoliation level. Egg weight and the levels of two yolk proteins, vitellin (Vt) and glycine-rich protein (GRP), however, had no influence on the proportion of ballooning larvae. The length of survival without food, and thus the maximum period of time for dispersal, was correlated with levels of Vt and GRP, but not with egg weight. The level of defoliation at the site from which the maternal parent was collected was not related to the longevity of offspring, nor did it have a significant effect on the levels of Vt, GRP or egg weight. Levels of hemolymph proteins arylphorin and vitellogenin in the maternal parent during the prepupal stage had no influence on levels of yolk proteins, larval longevity, or tendency to balloon.
Action Intentions Modulate Allocation of Visual Attention: Electrophysiological Evidence

In line with the Theory of Event Coding (Hommel et al., 2001), action planning has been shown to affect perceptual processing – an effect that has been attributed to a so-called intentional weighting mechanism (Wykowska et al., 2009; Hommel, 2010). This paper investigates the electrophysiological correlates of action-related modulations of selection mechanisms in visual perception. A paradigm combining a visual search task for size and luminance targets with a movement task (grasping or pointing) was introduced, and the EEG was recorded while participants were performing the tasks. The results showed that the behavioral congruency effects, i.e., better performance in congruent (relative to incongruent) action-perception trials have been reflected by a modulation of the P1 component as well as the N2pc (an ERP marker of spatial attention). These results support the argumentation that action planning modulates already early perceptual processing and attention mechanisms.
Identification of the physician workforce providing palliative care in Ontario using administrative claims data.

BACKGROUND Little is known about the physician workforce providing palliative care in Canada, and in Ontario specifically. We developed an algorithm to identify palliative care physicians using administrative claims data and validated it against a reference sample. We then applied the algorithm to all general practitioners/family physicians (GP/FPs) in the province of Ontario to describe and quantify those identified by the algorithm. METHODS W e reviewed Ontario Health Insurance Plan claims from Jan. 1, 2008, to Dec. 31, 2011, to determine each physician's proportion of claims that were for palliative care. We empirically selected a data-driven cut-off, whereby physicians whose proportion of palliative care claims was above the threshold were defined as palliative care physicians. We validated the cut-off against a reference sample of physicians who self-identified as providing mostly palliative care in a study-specific survey. We then applied this algorithm to all GP/FPs in the province. RESULTS We empirically selected 10% as the cut-off for the proportion of palliative care claims. This threshold had exceptional specificity and positive predictive value (97.8% and 90.5%, respectively) and adequate sensitivity (76.0%) when compared with the reference sample (n = 118). When applied to all GP/FPs in the province, the algorithm identified 276 practising mostly palliative care. Of these, 135 (48.9%) were women, 265 (96.0%) practised in urban locations, and 145 (52.5%) worked part time. INTERPRETATION Our algorithm readily identified and quantified the workforce of palliative care physicians in Ontario. Such a tool has numerous applications for both health service planners and researchers.
Optical CDMA using 2-D codes: the optimal single-user detector

We consider the error performance of an optical code-division multiple access network in which two-dimensional codes are generated in time and wavelength. We show from first principles that the optimum single-user detection scheme which yields is the AND detector. By replacing the widely considered SUM detector with the AND detector, the channel capacity can be at least doubled for a given data rate, number of active users, and bit error rate. We have also shown that the error performance of a random code gives a tight upper bound on the performance of deterministic code with the same weight and dimension.
PANDAA: physical arrangement detection of networked devices through ambient-sound awareness

Future ubiquitous home environments can contain 10s or 100s of devices. Ubiquitous services running on these devices (i.e. localizing users, routing, security algorithms) will commonly require an accurate location of each device. In order to obtain these locations, existing techniques require either a manual survey, active sound sources, or estimation using wireless radios. These techniques, however, need additional hardware capabilities and are intrusive to the user. Non-intrusive, automatic localization of ubiquitous computing devices in the home has the potential to greatly facilitate device deployments. This paper presents the PANDAA system, a zero-configuration spatial localization system for networked devices based on ambient sound sensing. After initial placement of the devices, ambient sounds, such as human speech, music, foot- steps, finger snaps, hand claps, or coughs and sneezes, are used to autonomously resolve the spatial relative arrangement of devices using trigonometric bounds and successive approximation. Using only time difference of arrival measurements as a bound for successive estimations, PANDAA is able to achieve an average of 0.17 meter accuracy for device location in the meeting room deployment.
Exploiting the potential of polyphenolic biomordants in environmentally friendly coloration of wool with natural dye from Butea monosperma flower extract

ABSTRACT Coloration of textiles with natural dyes involves huge consumption of chemicals; mainly, metal salt mordants, which pose serious environmental challenges. To mitigate this pollution, more and more environmentally friendly, biodegradable, biocompatible and renewable products should be popularized in dyeing industry. This paper proposes a sustainable and metal salt-free dyeing of wool samples by employing natural mordants extracted from pomegranate peel (Punica granatum L), gallnut (Quercus infectoria L) and catechu (Acacia catechu). The color parameters of dyed wool samples were greatly found to be dependent on the chemical compounds present in respective biomordants and their interaction ability with the functional groups of wool and the dye molecules. The investigated biomordants exhibit different interactions with coloring compounds of Butea monosperma (palas) dye resulting in deep brown, olive green, dark brown, cinnamon, burgundy, and yellowish hues on wool. All the biomordants selected in this study improved dye performance and resulted in a broad beautiful spectrum of colors with acceptable fastness properties. The results encourage the search and exploitation of new plant species as a source of biomordants to replace metallic and toxic mordants currently used in textile industry.