Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytotoxic T lymphocytes in protection against equine infectious anemia virus

Abstract Cytotoxic T lymphocytes (CTL) are associated with virus control in horses infected with equine infectious anemia virus (EIAV). Early in infection, control of the initial viremia coincides with the appearance of CTL and occurs before the appearance of neutralizing antibody. In carrier horses, treatment with immunosuppressive drugs results in viremia before a change in serum neutralizing antibody occurs. Clearance of initial viremia caused by other lentiviruses, including human immunodeficiency virus-1 and simian immunodeficiency virus, is also associated with CTL and not neutralizing antibody. In addition, depletion of CD8+ cells prior to infection of rhesus monkeys with simian immunodeficiency prevents clearance of virus and the same treatment of persistently infected monkeys results in viremia. Cats given adoptive transfers of lymphocytes from vaccinated cats were protected and the protection was MHC-restricted, occurred in the absence of antiviral humoral immunity, and correlated with the transfer of cells with feline immunodeficiency virus-specific CTL and T-helper lymphocyte activities. Therefore, a lentiviral vaccine, including one for EIAV, needs to induce CTL. Based on initial failures to induce CTL to EIAV proteins by any means other than infection, we attempted to define an experimental system for the evaluation of methods for CTL induction. CTL epitopes restricted by the ELA-A1 haplotype were identified and the MHC class I molecule presenting these peptides was identified. This was done by expressing individual MHC class I molecules from cDNA clones in target cells. The target cells were then pulsed with peptides and used with effector CTL stimulated with the same peptides. In a preliminary experiment, immunization of three ELA-A1 haplotype horses with an Env peptide restricted by this haplotype resulted in CTL in peripheral blood mononuclear cells (PBMC) which recognized the Env peptide and virus-infected cells, but the CTL response was transient. Nevertheless there was significant protection against clinical disease following EIAV challenge of these immunized horses when compared with three control horses given the same virus challenge. These data indicated that responses to peptides in immunized horses needed to be enhanced. Optimal CTL responses require help from CD4+ T lymphocytes, and experiments were done to identify EIAV peptides which stimulated CD4+ T lymphocytes in PBMC from infected horses with different MHC class II types. Two broadly cross-reactive Gag peptides were identified which stimulated only an interferon γ response by CD4+ T lymphocytes, which indicated a T helper 1 response is needed for CTL stimulation. Such peptides should facilitate CTL responses; however, other problems in inducing protection against lentiviruses remain, the most significant of them being EIAV variants that can escape both CTL and neutralizing antibody. A possible solution to CTL escape variants is the induction of high-avidity CTL to multiple EIAV epitopes.
AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane*♦

Extracellular Ca2+ is essential for the development of stable epithelial tight junctions. We find that in the absence of extracellular Ca2+, AMP-activated protein kinase (AMPK) activation and glycogen synthase kinase (GSK)-3β inhibition independently induce the localization of epithelial tight junction components to the plasma membrane. The Ca2+-independent deposition of junctional proteins induced by AMPK activation and GSK-3β inhibition is independent of E-cadherin. Furthermore, the nectin-afadin system is required for the deposition of tight junction components induced by AMPK activation, but it is not required for that induced by GSK-3β inhibition. Phosphorylation studies demonstrate that afadin is a substrate for AMPK. These data demonstrate that two kinases involved in regulating cell growth and metabolism act through distinct pathways to influence the deposition of the components of epithelial tight junctions.
Size and properties of the narrow-line region in Seyfert-1 galaxies from spatially-resolved optical spectroscopy

Context. Spatially resolved emission-line spectroscopy is a powerful tool for determining the physical conditions in the narrow-line region (NLR) of active galactic nuclei (AGNs). We recently used optical long-slit spectroscopy to study the NLRs of a sample of six Seyfert-2 galaxies. We have shown that such an approach, in comparison to the commonly used [O III] narrow-band imaging alone, allows us to probe the size of the NLR in terms of AGN photoionisation. Moreover, several physical parameters of the NLR can be directly accessed. Aims. We here apply the same methods to study the NLR of six Seyfert-1 galaxies and compare our results to those of Seyfert-2 galaxies. Methods. We employ diagnostically valuable emission-line ratios to determine the physical properties of the NLR, including the core values and radial dependencies of density, ionisation parameter, and reddening. Tracking the radial change of emission-line ratios in diagnostic diagrams allows us to measure the transition between AGN-like and H II-like line excitation, and thus to measure the size of the NLR. Results. In the diagnostic diagrams, we find a transition between line ratios falling in the AGN regime and those typical of H II regions in two Seyfert-1 galaxies, thereby determining the size of the NLR. The central electron temperature and ionisation parameter are, in general, higher in type-1 Seyferts than in type 2s. In almost all cases, both electron density and ionisation parameter decrease with radius and the decrease is faster in Seyfert-1 galaxies than in Sy2s. In several objects, the gaseous velocity distribution is characteristic of rotational motion in an (inclined) emission-line disk in the centre. We give estimates of the black-hole masses and discuss our findings in detail for each object.
Supratrigeminal Bilaterally Projecting Neurons Maintain Basal Tone and Enable Bilateral Phasic Activation of Jaw-Closing Muscles

Anatomical studies have identified brainstem neurons that project bilaterally to left and right oromotor pools, which could potentially mediate bilateral muscle coordination. We use retrograde lentiviruses combined with a split-intein-mediated split-Cre-recombinase system in mice to isolate, characterize, and manipulate a population of neurons projecting to both the left and right jaw-closing trigeminal motoneurons. We find that these bilaterally projecting premotor neurons (BPNs) reside primarily in the supratrigeminal nucleus (SupV) and the parvicellular and intermediate reticular regions dorsal to the facial motor nucleus. These BPNs also project to multiple midbrain and brainstem targets implicated in orofacial sensorimotor control, and consist of a mix of glutamatergic, GABAergic, and glycinergic neurons, which can drive both excitatory and inhibitory inputs to trigeminal motoneurons when optogenetically activated in slice. Silencing BPNs with tetanus toxin light chain (TeNT) increases bilateral masseter activation during chewing, an effect driven by the expression of TeNT in SupV BPNs. Acute unilateral optogenetic inhibition of SupV BPNs identifies a group of tonically active neurons that function to lower masseter muscle tone, whereas unilateral optogenetic activation of SupV BPNs is sufficient to induce bilateral masseter activation both during resting state and during chewing. These results provide evidence for SupV BPNs in tonically modulating jaw-closing muscle tone and in mediating bilateral jaw closing. SIGNIFICANCE STATEMENT We developed a method that combines retrograde lentiviruses with the split-intein-split-Cre system in mice to isolate, characterize, and manipulate neurons that project to both left and right jaw-closing motoneurons. We show that these bilaterally projecting premotor neurons (BPNs) reside primarily in the supratrigeminal nucleus and the rostral parvicellular and intermediate reticular nuclei. BPNs consist of both excitatory and inhibitory populations, and also project to multiple brainstem nuclei implicated in orofacial sensorimotor control. Manipulation of the supratrigeminal BPNs during natural jaw-closing behavior reveals a dual role for these neurons in eliciting phasic muscle activation and in maintaining basal muscle tone. The retrograde lentivirus carrying the split-intein-split-Cre system can be applied to study any neurons with bifurcating axons innervating two brain regions.
Coverage distribution of heterogeneous cellular networks under unsaturated load

Heterogeneous cellular networks have received considerable attention in the literature owing to increased data demands of cellular network users. Recently, Poisson Point processes (PPPs) are vastly used for modeling and analyzing cellular networks because of their accuracy and tractability. However, results in this area suffer from a big drawback. They considered a saturated load model in their analysis or used a base station (BS) activity factor independent of the network condition. In this work, we evaluate the performance of cellular networks under a more accurate definition of load. In cellular networks, a BS is active when it has at least one user associated and is inactive when it has no associated user. The load definition used in this paper is a user-based load model and denotes whether at least one user is associated with the BS or not. This paper provides a closed form relation for the probability of inactive BSs of each tier. Based on this value, the coverage probability of the network has been derived. Finally, the performance of analytical expressions has been validated with simulations.
A real-time parametric stiffness observer for VSA devices

We consider the problem of estimating non-linear time-varying stiffness of a mechanical system based only on force and position measurements. A recent work presented a non-parametric stiffness observer, which converges to within an Uniformly Ultimately Bounded neighborhood of the real stiffness value. The method provides excellent results for applications where the system is persistently excited. In this paper, we provide a parametric identification method that complements the previous solution in that it can provide, after a sufficiently long learning period, a complete model of the nonlinear stiffness, which can be applied henceforth even in the absence of excitation. Convergence conditions for the proposed method are discussed. Simulation and experimental results are provided, illustrating the performance of the proposed algorithm.
AMONG HOMELESS YOUTH 1 Running head : DATING VIOLENCE AMONG HOMELESS YOUTH Effects of Abusive Parenting , Caretaker Arrests , and Deviant Behavior on Dating Violence among Homeless Young Adults

Though dating violence is widespread among young adult homeless populations, its risk factors are poorly understood by scholars. To address this gap, the current study uses a social learning theory to examine the effects of abusive parenting and caretaker arrests on dating violence among 172 homeless young adults. Results from path analyses revealed that child physical abuse and caretaker arrests were positively associated with engaging in a greater number of school fights, which, in turn, was strongly and positively correlated with participating in more deviant subsistence strategies (e.g., stealing) since being on the street. Young people who participated in a greater number of delinquent acts were more likely to report higher levels of dating violence. Study results highlight the extent of social learning within the lives of homeless young adults, which is evident prior to their leaving home and while they are on the street.
The limb movement analysis of rehabilitation exercises using wearable inertial sensors

Due to no supervision of a therapist in home based exercise programs, inertial sensor based feedback systems which can accurately assess movement repetitions are urgently required. The synchronicity and the degrees of freedom both show that one movement might resemble another movement signal which is mixed in with another not precisely defined movement. Therefore, the data and feature selections are important for movement analysis. This paper explores the data and feature selection for the limb movement analysis of rehabilitation exercises. The results highlight that the classification accuracy is very sensitive to the mount location of the sensors. The results show that the use of 2 or 3 sensor units, the combination of acceleration and gyroscope data, and the feature sets combined by the statistical feature set with another type of feature, can significantly improve the classification accuracy rates. The results illustrate that acceleration data is more effective than gyroscope data for most of the movement analysis.
Polymer blend and nucleating agent: on structural evolution and space charge on polyethylene (PE) blends and Dibenzylidene Sorbitol

This paper examines the effects of 1,3:2,4-dibenzylidene sorbitol (DBS), a low-molecular organic molecule, on polyethylene space charge distribution. Morphological studies of the blends show the presence of a DBS network in the host system. Avrami analysis shows an increase in the nucleation density of PE with the nucleating agent. Accumulation of space charge is one of the main causes of failure for high voltage cables and it depends on many factors such as additives, materials and treatments. In this study we investigate the effects of different quantities of DBS in a blend of polyethylene and their space charge responses.
Association of Tinnitus and Electromagnetic Hypersensitivity: Hints for a Shared Pathophysiology?

Background Tinnitus is a frequent condition with high morbidity and impairment in quality of life. The pathophysiology is still incompletely understood. Electromagnetic fields are discussed to be involved in the multi-factorial pathogenesis of tinnitus, but data proofing this relationship are very limited. Potential health hazards of electromagnetic fields (EMF) have been under discussion for long. Especially, individuals claiming themselves to be electromagnetic hypersensitive suffer from a variety of unspecific symptoms, which they attribute to EMF-exposure. The aim of the study was to elucidate the relationship between EMF-exposure, electromagnetic hypersensitivity and tinnitus using a case-control design. Methodology Tinnitus occurrence and tinnitus severity were assessed by questionnaires in 89 electromagnetic hypersensitive patients and 107 controls matched for age-, gender, living surroundings and workplace. Using a logistic regression approach, potential risk factors for the development of tinnitus were evaluated. Findings Tinnitus was significantly more frequent in the electromagnetic hypersensitive group (50.72% vs. 17.5%) whereas tinnitus duration and severity did not differ between groups. Electromagnetic hypersensitivity and tinnitus were independent risk factors for sleep disturbances. However, measures of individual EMF-exposure like e.g. cell phone use did not show any association with tinnitus. Conclusions Our data indicate that tinnitus is associated with subjective electromagnetic hypersensitivity. An individual vulnerability probably due to an over activated cortical distress network seems to be responsible for, both, electromagnetic hypersensitivity and tinnitus. Hence, therapeutic efforts should focus on treatment strategies (e.g. cognitive behavioral therapy) aiming at normalizing this dysfunctional distress network.
A Formal Framework for Speedup Learning from Problems and Solutions

Speedup learning seeks to improve the computational efficiency of problem solving with experience. In this paper, we develop a formal framework for learning efficient problem solving from random problems and their solutions. We apply this framework to two different representations of learned knowledge, namely control rules and macro-operators, and prove theorems that identify sufficient conditions for learning in each representation. Our proofs are constructive in that they are accompanied with learning algorithms. Our framework captures both empirical and explanation-based speedup learning in a unified fashion. We illustrate our framework with implementations in two domains: symbolic integration and Eight Puzzle. This work integrates many strands of experimental and theoretical work in machine learning, including empirical learning of control rules, macro-operator learning, Explanation-Based Learning (EBL), and Probably Approximately Correct (PAC) Learning.
Lateralization of music processing with noises in the auditory cortex: an fNIRS study

The present study is to determine the effects of background noise on the hemispheric lateralization in music processing by exposing 14 subjects to four different auditory environments: music segments only, noise segments only, music + noise segments, and the entire music interfered by noise segments. The hemodynamic responses in both hemispheres caused by the perception of music in 10 different conditions were measured using functional near-infrared spectroscopy. As a feature to distinguish stimulus-evoked hemodynamics, the difference between the mean and the minimum value of the hemodynamic response for a given stimulus was used. The right-hemispheric lateralization in music processing was about 75% (instead of continuous music, only music segments were heard). If the stimuli were only noises, the lateralization was about 65%. But, if the music was mixed with noises, the right-hemispheric lateralization has increased. Particularly, if the noise was a little bit lower than the music (i.e., music level 10~15%, noise level 10%), the entire subjects showed the right-hemispheric lateralization: This is due to the subjects' effort to hear the music in the presence of noises. However, too much noise has reduced the subjects' discerning efforts.
Tissue transglutaminase antibodies in celiac disease

OBJECTIVE:Tissue transglutaminase is the antigen for antiendomysial antibodies, whose power in screening for celiac disease is well known. Our aim was to assess the efficacy of an ELISA assay for tissue transglutaminase antibodies.METHODS:Tissue transglutaminase antibodies were analyzed in serum from 39 untreated celiac disease patients and 61 controls. Tissue transglutaminase was used as antigen, and test sera analyzed by ELISA. Results higher than 0.6 optical density were considered positive, lower than 0.4 negative, and between 0.4 and 0.6 borderline.RESULTS:Optical density of the serum from the patients with untreated celiac disease (median: 1.41; range: 0.33–1.47) were significantly higher than the controls (median: 0.32; range: 0.17–0.68; p < 0.0001; 95% confidence interval 0.87–1.08). Thirty-three patients with untreated celiac disease were positive, 4 borderline, and 2 negative. Fifty-five controls were negative, 4 borderline, and 2 positive. If we consider borderline results to be positive, sensitivity is 94.8% and specificity 90.1%. None of the controls gave results higher than 0.7 optical density. Apart from the 2 negative patients with untreated celiac disease, the two groups overlapped only between 0.4 and 0.7 optical density.CONCLUSIONS:Because of the high sensitivity (∼95%) and technical simplicity, tissue transglutaminase antibodies may prove useful for the screening of celiac disease in population at low or medium risk of celiac disease. To avoid duodenal biopsies in patients without celiac disease, the specificity of the screening procedure may be increased by confirming with antiendomysial antibodies by immunofluorescence on human umbilical cord in individuals with results between 0.4 and 0.7 optical density.
Essential role of non-canonical Wnt signalling in neural crest migration

Migration of neural crest cells is an elaborate process that requires the delamination of cells from an epithelium and cell movement into an extracellular matrix. In this work, it is shown for the first time that the non-canonical Wnt signalling [planar cell polarity (PCP) or Wnt-Ca2+] pathway controls migration of neural crest cells. By using specific Dsh mutants, we show that the canonical Wnt signalling pathway is needed for neural crest induction, while the non-canonical Wnt pathway is required for neural crest migration. Grafts of neural crest tissue expressing non-canonical Dsh mutants, as well as neural crest cultured in vitro, indicate that the PCP pathway works in a cell-autonomous manner to control neural crest migration. Expression analysis of non-canonical Wnt ligands and their putative receptors show that Wnt11 is expressed in tissue adjacent to neural crest cells expressing the Wnt receptor Frizzled7 (Fz7). Furthermore, loss- and gain-of-function experiments reveal that Wnt11 plays an essential role in neural crest migration. Inhibition of neural crest migration by blocking Wnt11 activity can be rescued by intracellular activation of the non-canonical Wnt pathway. When Wnt11 is expressed opposite its normal site of expression, neural crest migration is blocked. Finally, time-lapse analysis of cell movement and cell protrusion in neural crest cultured in vitro shows that the PCP or Wnt-Ca2+ pathway directs the formation of lamellipodia and filopodia in the neural crest cells that are required for their delamination and/or migration.
Comparison of Macintosh, Truview EVO2, Glidescope, and Airwayscope laryngoscope use in patients with cervical spine immobilization.

BACKGROUND The purpose of this study was to evaluate the effectiveness of the Pentax AWS, Glidescope, and the Truview EVO2, in comparison with the Macintosh laryngoscope, when performing tracheal intubation in patients with neck immobilization using manual in-line axial cervical spine stabilization. METHODS One hundred and twenty consenting patients presenting for surgery requiring tracheal intubation were randomly assigned to undergo intubation using a Macintosh (n=30), Glidescope (n=30), Truview EVO2 (n=30), or AWS (n=30) laryngoscope. All patients were intubated by one of the three anaesthetists experienced in the use of each laryngoscope. RESULTS The Glidescope, AWS, and Truview EVO2 each reduced the intubation difficulty score (IDS), improved the Cormack and Lehane glottic view, and reduced the need for optimization manoeuvres, compared with the Macintosh. The mean IDS was significantly lower with the Glidescope and AWS compared with the Truview EVO2 device, and the IDS was lowest with the AWS. The duration of tracheal intubation attempts was significantly shorter with the Macintosh compared with the other devices. There were no differences in success rates between the devices tested. The AWS produced the least haemodynamic stimulation. CONCLUSIONS The Glidescope and AWS laryngoscopes required more time but reduced intubation difficulty and improved glottic view over the Macintosh laryngoscope more than the Truview EVO2 laryngoscope when used in patients undergoing cervical spine immobilization.