Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact of USA300 methicillin-resistant Staphylococcus aureus on clinical outcomes of patients with pneumonia or central line-associated bloodstream infections.

BACKGROUND The USA300 methicillin-resistant Staphylococcus aureus (MRSA) strain, which initially emerged as a cause of community-associated infections, has recently become an important pathogen in healthcare-associated infections (HAIs). However, its impact on patient outcomes has not been well studied. We evaluated patients with invasive MRSA infections to assess differences in outcomes between infections caused by USA100 and those caused by USA300. METHODS Population-based data for invasive MRSA infections were used to identify 2 cohorts: (1) nondialysis patients with central line-associated bloodstream infections (CLABSIs) and (2) patients with community-onset pneumonia (PNEUMO) during 2005-2007 from 6 US metropolitan areas. Medical records of patients with confirmed MRSA USA100 or USA300 infection were reviewed. Logistic regression and, when appropriate, survival analysis was performed to evaluate mortality, early and late complications, and length of stay. RESULTS A total of 236 and 100 patients were included in the CLABSI and PNEUMO cohorts, respectively. USA300 was the only independent predictor of early complications for PNEUMO patients (odds ratio [OR], 2.6; P = .02). Independent predictors of CLABSI late complications included intensive care unit (ICU) admission before MRSA culture (adjusted OR [AOR], 2.1; P= .01) and Charlson comorbidity index (AOR, 2.6; P = .003), but not strain type. PNEUMO patients were significantly more likely to die if they were older (P = .02), black (P < .001), or infected with USA100 strain (P = .02), whereas those with CLABSI were more likely to die if they were older (P < .001), had comorbidities (P < .001), or had an ICU admission before MRSA culture (P = .001). CONCLUSIONS USA300 was associated with early complications in PNEUMO patients. However, it was not associated with mortality for either PNEUMO or CLABSI patients. Concerns regarding higher mortality from HAIs caused by USA300 may not be warranted.
Automated Telephone Calls Improved Completion of Fecal Occult Blood Testing

Background:Although colorectal cancer (CRC) prognosis is improved by early diagnosis, screening rates remain low. Objective:To determine the effect of an automated telephone intervention on completion of fecal occult blood testing (FOBT). Research Design:In this randomized controlled trial conducted at Kaiser Permanente Northwest, a not-for-profit health maintenance organization, 5905 eligible patients aged 51 to 80, at average risk for CRC and due for CRC screening, were randomly assigned to an automated telephone intervention (n = 2943) or usual care (UC; n = 2962). The intervention group received up to three 1-minute automated telephone calls that provided a description and health benefits of FOBT. During the call, patients could request that an FOBT kit be mailed to their home. Those who requested but did not return the cards received an automated reminder call. Cox proportional hazard method was used to determine the independent effect of automated telephone calls on completion of an FOBT, after adjusting for age, sex, and prior CRC screening. Results:By 6 months after call initiation, 22.5% in the intervention and 16.0% in UC had completed an FOBT. Those in the intervention group were significantly more likely to complete an FOBT (hazard ratio, 1.31; 95% confidence interval, 1.10–1.56) compared with UC. Older patients (aged 71–80 vs. aged 51–60) were also more likely to complete FOBT (hazard ratio, 1.48; 95% confidence interval, 1.07–2.04). Conclusions:Automated telephone calls increased completion of FOBT. Further research is needed to evaluate automated telephone interventions among diverse populations and in other clinical settings.
Phylogenetic diversity and functional gene patterns of sulfur-oxidizing subseafloor Epsilonproteobacteria in diffuse hydrothermal vent fluids

Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vents over a range of geochemical gradients from Axial Seamount, a deep-sea volcano in the Northeast Pacific. PCR primers were developed and applied to target the sulfur oxidation soxB gene of Epsilonproteobacteria. soxB genes belonging to the genera Sulfurimonas and Sulfurovum are both present and expressed at most diffuse vent sites, but not in background seawater. Although Sulfurovum-like soxB genes were detected in all fluid samples, the RNA profiles were nearly identical among the vents and suggest that Sulfurimonas-like species are the primary Epsilonproteobacteria responsible for actively oxidizing sulfur via the Sox pathway at each vent. Community patterns of subseafloor Epsilonproteobacteria 16S rRNA genes were best matched to methane concentrations in vent fluids, as well as individual vent locations, indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations. The data show that in the subseafloor at Axial Seamount, Epsilonproteobacteria are expressing the soxB gene and that microbial patterns in community distribution are linked to both vent location and chemistry.
Inhibitory Effect of Flavonoids on the Efflux of N-Acetyl 5-Aminosalicylic Acid Intracellularly Formed in Caco-2 Cells

N-acetyl 5-aminosalicylic acid (5-AcASA) that was intracellularly formed from 5-aminosalicylic acid (5-ASA) at 200 μM was discharged 5.3, 7.1, and 8.1-fold higher into the apical site than into the basolateral site during 1, 2, and 4-hour incubations, respectively, in Caco-2 cells grown in Transwells. The addition of flavonols (100 μM) such as fisetin and quercetin with 5-ASA remarkably decreased the apically directed efflux of 5-AcASA. When 5-ASA (200 μM) was added to Caco-2 cells grown in tissue culture dishes, the formation of 5-AcASA decreased, and, in addition, the formed 5-AcASA was found to be accumulated within the cells in the presence of such flavonols. Thus, the decrease in 5-AcASA efflux by such flavonols was attributed not only to the inhibition of N-acetyl-conjugation of 5-ASA but to the predominant cellular accumulation of 5-AcASA. Various flavonoids also had both of the effects with potencies that depend on their specific structures. The essential structure of flavonoids was an absence of a hydroxyl substitution at the C5 position on the A-ring of flavone structure for the inhibitory effect on the N-acetyl-conjugation of 5-ASA, and a presence of hydroxyl substitutions at the C3′ or C4′ position on the B-ring of flavone structure for the promoting effect on the cellular accumulation of 5-AcASA. Both the decrease in 5-AcASA apical efflux and the increase in 5-AcASA cellular accumulation were also caused by MK571 and indomethacin, inhibitors of MRPs, but not by quinidine, cyclosporin A, P-glycoprotein inhibitors, and mitoxantrone, a BCRP substrate. These results suggest that certain flavonoids suppress the apical efflux of 5-AcASA possibly by inhibiting MRPs pumps located on apical membranes in Caco-2 cells.
Pass-Through of External Shocks to Euro Area Inflation

This paper investigates the pass-through of external shocks, i.e. oil price shocks, exchange rate shocks, and non-oil import price shocks to euro area inflation at different stages of distribution (import prices, producer prices and consumer prices). The analysis is based on a VAR model that includes the distribution chain of pricing. According to our results the pass-through is largest and fastest for non-oil import price shocks, followed by exchange rate shocks and oil price shocks. The size and the speed of the pass-through of these shocks decline along the distribution chain. External shocks explain a large fraction of the variance in all price indices. They seem to have contributed largely to inflation in the euro area since the start of the European Monetary Union. The results on the size and the speed of the pass-through in the euro area appeared to be robust over time and different identification schemes. JEL Classification: C32, E31
The Nature, Meaning, and Measure of Teacher Flow in Elementary Schools: A Test of Rival Hypotheses

Purpose: This inquiry is the first comprehensive, empirical analysis of the nature and measurement of flow in elementary teachers. The clearest sign of flow is the merging of action and awareness, that is, the degree to which an activity becomes spontaneous and automatic and individuals lose conscious awareness of themselves as they perform a task such as teaching. The basic objective of the research was to examine the theoretical structure and measurement of flow in elementary teachers. Research Methods: A typical sample of 260 elementary teachers from rural, urban, and suburban elementary schools in Ohio was used to test two rival explanations about the nature of flow. Structural equation modeling was used to assess the goodness of fit of the two models. Findings: Two rival explanations of flow, the Jackson-Marsh and the Quinn models, were evaluated using confirmatory factor analysis and structural equation modeling. The Jackson-Marsh model of flow proved to be a better explanation of flow of elementary teachers than the Quinn model. Consistent with Csikszentmihalyi’s explanation of flow, the construct consists of nine elements, all of which form an integrated whole. In addition, and as predicted, optimism was positively related to flow. Discussion: Because most flow research has been in sports and leisure, Quinn’s research in knowledge work seemed especially relevant for the study of flow in teachers. In spite of that fact, the Jackson and Marsh model was the better fit; the Quinn model provided insight into the dynamics of flow in elementary schools. Finally, although optimism was positively related to flow, it was academic optimism, not dispositional optimism, that was a strong predictor of flow in elementary teachers. Conclusions: The nature of flow in schools is instructive, offering insight into the elements of flow and how they collectively and individually inform us in pursuit of optimal teaching and learning conditions, but much more research remains to be done.
Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella

Intraflagellar transport (IFT), which is the bidirectional movement of particles within flagella, is required for flagellar assembly. IFT particles are composed of ∼16 proteins, which are organized into complexes A and B. We have cloned Chlamydomonas reinhardtii and mouse IFT46, and show that IFT46 is a highly conserved complex B protein in both organisms. A C. reinhardtii insertional mutant null for IFT46 has short, paralyzed flagella lacking dynein arms and with central pair defects. The mutant has greatly reduced levels of most complex B proteins, indicating that IFT46 is necessary for complex B stability. A partial suppressor mutation restores flagellar length to the ift46 mutant. IFT46 is still absent, but levels of the other IFT particle proteins are largely restored, indicating that complex B is stabilized in the suppressed strain. Axonemal ultrastructure is restored, except that the outer arms are still missing, although outer arm subunits are present in the cytoplasm. Thus, IFT46 is specifically required for transporting outer arms into the flagellum.
Sexual professionalism: for whom? The case of sexual facilitation in Swedish personal assistance services

Sexuality is a taboo subject in disability services, leading to insecurity for both service users and personnel about how to handle upcoming situations. In Sweden, there is also a lack of policy in this area, highlighting the need to study sexuality both as an individual and a political, and in this case also, depoliticized issue. A critical feminist policy analysis reveals that norms around disability, sexuality and professionalism in a particular legal, political and cultural context strongly influence the willingness to recognize disabled people’s sexual rights. The Swedish case indicates a need for increased transnational work to develop ethical, professional and non-discriminatory rights-based approaches to sexual facilitation.
Early childhood development in South Africa – progress since the end of apartheid

In April 1994, South Africa held its historic first democratic election. The African National Congress overwhelmingly triumphed and Nelson Mandela became the first president of a free and democratic South Africa. In this review, the situation of South Africa's young children under apartheid and the context of young children in South Africa in 2012 are described. A situation analysis of early childhood development (ECD) in South Africa was undertaken using South African government ECD policy and programme implementation reports, and the main challenges affecting children and the ECD sector in South Africa was investigated. There has been progress since 1994, both quantitatively and qualitatively. Children now have access to a Grade R year, government education and social development budgets have increased and a per capita subsidy is available to qualifying children at ECD centres nationally. More children are in provision and in better-quality provision than before. However, much still remains to be done before we can say with confidence that the needs of our youngest children are being met. This study identifies infrastructure, nutrition, ECD programmes, teacher training, institutional capacity and funding as the major gaps in ECD provision.
Should schools be optimistic? An investigation of the association between academic optimism of schools and student achievement in primary education

Academic emphasis, collective efficacy, and faculty trust in students and parents (3 school characteristics positively associated with student achievement) are assumed to form a higher order latent construct, “academic optimism” (Hoy, Tarter, & Woolfolk Hoy, 2006a, 2006b). The aim of the present study is to corroborate the latent factor structure of academic optimism and to investigate its association with student achievement in Flanders while taking the hierarchical structure of the data into account. Data from 1,375 staff members and 3,538 fifth-grade students within 117 schools were analysed using multilevel confirmatory factor analysis and multilevel structural equation modelling. The results indicated that all three school characteristics make up one latent school construct, “academic optimism”. Academic optimism was found to be positively associated with student achievement in mathematics and reading comprehension. School mean socioeconomic status and school mean prior achievement are mainly indirectly associated with student achievement through academic optimism.
SVD-Based Quality Metric for Image and Video Using Machine Learning

We study the use of machine learning for visual quality evaluation with comprehensive singular value decomposition (SVD)-based visual features. In this paper, the two-stage process and the relevant work in the existing visual quality metrics are first introduced followed by an in-depth analysis of SVD for visual quality assessment. Singular values and vectors form the selected features for visual quality assessment. Machine learning is then used for the feature pooling process and demonstrated to be effective. This is to address the limitations of the existing pooling techniques, like simple summation, averaging, Minkowski summation, etc., which tend to be ad hoc. We advocate machine learning for feature pooling because it is more systematic and data driven. The experiments show that the proposed method outperforms the eight existing relevant schemes. Extensive analysis and cross validation are performed with ten publicly available databases (eight for images with a total of 4042 test images and two for video with a total of 228 videos). We use all publicly accessible software and databases in this study, as well as making our own software public, to facilitate comparison in future research.
Obesity comorbidity in unipolar major depressive disorder: refining the core phenotype.

OBJECTIVE While a significant body of research has demonstrated high comorbidity rates between depression and obesity, the vast majority of this work has considered depression as a unitary diagnosis. Given that increased appetite and weight gain are highly characteristic of the "atypical" subtype of depression, while classic depression is characterized by decreased appetite and weight loss, it would be important to examine whether increased obesity risk is consistent across the major vegetative subtypes of depression or is limited to the atypical subtype. METHOD Using data from the 2001-2002 National Epidemiologic Survey on Alcohol and Related Conditions (NESARC), we identified 5,092 US adults with past or current major depression based on DSM-IV-TR criteria and 1,500 gender-matched controls. Each depressed subject was designated as having classic, atypical, or undifferentiated depression based on core vegetative symptoms. Logistic regression models examined rates of current obesity (defined as a current body mass index [kg/m2] > 30) across the 3 depressive subgroups and nondepressed controls, adjusting for demographic differences. To limit the possible effect of current depressive symptoms on observed obesity rates, secondary analyses were completed in individuals with past depression only. RESULTS Subjects with atypical depression had markedly elevated obesity rates compared to population controls and to other depressed subjects, with corresponding pairwise odds ratios consistently greater than 2.0 (P < .001). In contrast, obesity rates were not significantly different in subjects with classic depression and nondepressed controls. These results were manifest in individuals with either current or past depression and were independent of gender and age. CONCLUSIONS While many individuals with classic depression will present with obesity due to the high prevalence of both disorders, only atypical depression is associated with an elevated risk of obesity relative to the population at large. Refining the target phenotype(s) for future work on depression and obesity might improve our understanding, prevention, and treatment of this complex clinical problem.
Retrieval-induced forgetting: A developmental study

Two studies examined the possibility of retrieval-induced forgetting by 7-year-olds. Children heard a story while viewing pictures of events mentioned in the story, each highlighting objects drawn from two distinct semantic categories (e.g. animals and food). Over the next several days, children were asked the same yes/no questions about half the examples from one category and, finally, were tested for their memory of the complete set of examples from both categories. Both a category-cued recall test (Study 1) and a written recognition-memory test (Study 2) produced results suggestive of retrieval-induced forgetting. That is, children showed poorer memory for unpractised items from the practised category than for unpractised items from the other category. The severity of the effect did not differ reliably between the children and young adults, and was unaffected by whether practised items appeared before or after the unpractised competitors (Study 2). We consider the implications of these findings for competing views about retrieval-induced forgetting and the development of cognitive inhibition.
Efficient Bilevel Approach for Urban Rail Transit Operation With Stop-Skipping

The train scheduling problem for urban rail transit systems is considered with the aim of minimizing the total travel time of passengers and the energy consumption of the trains. We adopt a model-based approach, where the model includes the operation of trains at the terminus and at the stations. In order to adapt the train schedule to the origin-destination-dependent passenger demand in the urban rail transit system, a stop-skipping strategy is adopted to reduce the passenger travel time and the energy consumption. An efficient bilevel optimization approach is proposed to solve this train scheduling problem, which actually is a mixed-integer nonlinear programming problem. The performance of the new efficient bilevel approach is compared with the existing bilevel approach. In addition, we also compare the stop-skipping strategy with the all-stop strategy. The comparison is performed through a case study inspired by real data from the Beijing Yizhuang line. The simulation results show that the efficient bilevel approach and the existing bilevel approach have a similar performance but the computation time of the efficient bilevel approach is around one magnitude smaller than that of the bilevel approach.
The TEA Transcription Factor Tec1 Confers Promoter-Specific Gene Regulation by Ste12-Dependent and -Independent Mechanisms

ABSTRACT In Saccharomyces cerevisiae, the TEA transcription factor Tec1 is known to regulate target genes together with a second transcription factor, Ste12. Tec1-Ste12 complexes can activate transcription through Tec1 binding sites (TCSs), which can be further combined with Ste12 binding sites (PREs) for cooperative DNA binding. However, previous studies have hinted that Tec1 might regulate transcription also without Ste12. Here, we show that in vivo, physiological amounts of Tec1 are sufficient to stimulate TCS-mediated gene expression and transcription of the FLO11 gene in the absence of Ste12. In vitro, Tec1 is able to bind TCS elements with high affinity and specificity without Ste12. Furthermore, Tec1 contains a C-terminal transcriptional activation domain that confers Ste12-independent activation of TCS-regulated gene expression. On a genome-wide scale, we identified 302 Tec1 target genes that constitute two distinct classes. A first class of 254 genes is regulated by Tec1 in a Ste12-dependent manner and is enriched for genes that are bound by Tec1 and Ste12 in vivo. In contrast, a second class of 48 genes can be regulated by Tec1 independently of Ste12 and is enriched for genes that are bound by the stress transcription factors Yap6, Nrg1, Cin5, Skn7, Hsf1, and Msn4. Finally, we find that combinatorial control by Tec1-Ste12 complexes stabilizes Tec1 against degradation. Our study suggests that Tec1 is able to regulate TCS-mediated gene expression by Ste12-dependent and Ste12-independent mechanisms that enable promoter-specific transcriptional control.
Collective remembering of organizations: Co-construction of organizational pasts in Wikipedia

Purpose – How organizations’ pasts are presented to the public is crucial, because this presentation shapes corporate reputations. Increasingly, various actors contribute to the public remembering of organizations with new information and communication technologies (ICTs). The purpose of this paper is to investigate the online encyclopedia Wikipedia as a global memory place, where the pasts of organizations are communicatively co-constructed by actors of a loosely connected community. Design/methodology/approach – The authors analyze 1,459 edits of Wikipedia pages of ten organizations from various industries. Quantitative content analysis detects Wikipedia edits for their reputational relevance and reference to formal sources, such as corporate communication or newspapers. Furthermore, the authors investigate to which degree current corporate communication in form of 177 press releases has an influence on the remembering process in Wikipedia. Findings – The analysis shows how the continuous construction o.