Miracle-dz/UniFAR-FTUs huggingface dataset:
Complement Pathway Is Frequently Altered in Endometriosis and Endometriosis-Associated Ovarian Cancer

Purpose: Mechanisms of immune dysregulation associated with advanced tumors are relatively well understood. Much less is known about the role of immune effectors against cancer precursor lesions. Endometrioid and clear-cell ovarian tumors partly derive from endometriosis, a commonly diagnosed chronic inflammatory disease. We performed here a comprehensive immune gene expression analysis of pelvic inflammation in endometriosis and endometriosis-associated ovarian cancer (EAOC). Experimental Design: RNA was extracted from 120 paraffin tissue blocks comprising of normal endometrium (n = 32), benign endometriosis (n = 30), atypical endometriosis (n = 15), and EAOC (n = 43). Serous tumors (n = 15) were included as nonendometriosis-associated controls. The immune microenvironment was profiled using Nanostring and the nCounter GX Human Immunology Kit, comprising probes for a total of 511 immune genes. Results: One third of the patients with endometriosis revealed a tumor-like inflammation profile, suggesting that cancer-like immune signatures may develop earlier, in patients classified as clinically benign. Gene expression analyses revealed the complement pathway as most prominently involved in both endometriosis and EAOC. Complement proteins are abundantly present in epithelial cells in both benign and malignant lesions. Mechanistic studies in ovarian surface epithelial cells from mice with conditional (Cre-loxP) mutations show intrinsic production of complement in epithelia and demonstrate an early link between Kras- and Pten-driven pathways and complement upregulation. Downregulation of complement in these cells interferes with cell proliferation. Conclusions: These findings reveal new characteristics of inflammation in precursor lesions and point to previously unknown roles of complement in endometriosis and EAOC. Clin Cancer Res; 20(23); 6163–74. ©2014 AACR.
Mass distribution in the most X-ray-luminous galaxy cluster RX J1347.5-1145 studied with XMM-Newton

Context. We report on the analysis of XMM-Newton observations of RX J1347.5−1145 (z = 0.451), the most X-ray-luminous galaxy cluster. Aims. We present a detailed total and gas mass determination up to large distances (∼1.7 Mpc), study the scaling properties of the cluster, and explore the role of AGN heating in the cluster cool core. Methods. By means of spatially resolved spectroscopy we derive density, temperature, entropy, and cooling time profiles of the intracluster medium. We compute the total mass profile of the cluster in the assumption of hydrostatic equilibrium. Results. If the disturbed south-east region of the cluster is excluded from the analysis, our results on shape, normalization, scaling properties of density, temperature, entropy, and cooling time profiles are fully consistent with those of relaxed, cool core clusters. We compare our total and gas mass estimates with previous X-ray, lensing, dynamical, and SZ studies. We find good agreement with other X-ray results, dynamical mass measurements, weak lensing masses and SZ results. We confirm a discrepancy of a factor ∼2 between strong lensing and X-ray mass determinations and find a gross mismatch between our total mass estimate and the mass reconstructed through the combination of both strong and weak lensing. We explore the effervescent heating scenario in the core of RX J1347.5−1145 and find support to the picture that AGN outflows and heat conduction are able to quench radiative cooling.
Supplement to : The Diagnosis And Treatment of Heel Pain

Heel pain, whether plantar or posterior, is predominantly a mechanical pathology although an array of diverse pathologies including neurologic, arthritic, traumatic, neoplastic, infectious, or vascular etiologies must be considered. This clinical practice guideline (CPG) is a revision of the original 2001 document developed by the American College of Foot and Ankle Surgeons (ACFAS) heel pain committee. 2010 by the American College of Foot and Ankle Surgeons. All rights reserved. This clinical practice guideline (CPG) is based on consensus of current clinical practice and review of the clinical literature. The guideline was developed by the CPG Heel Pain Committee of the American College of Foot and Ankle Surgeons (ACFAS). This is the 2010 revision of the original 2001 CPG document published in the Journal of Foot & Ankle Surgery (Vol. 40, No. 5, pages 329–340). The guideline and references annotate each node of the corresponding
Transduction of the Nitrogen Signal Activating Gln3-mediated Transcription Is Independent of Npr1 Kinase and Rsp5-Bul1/2 Ubiquitin Ligase in Saccharomyces cerevisiae*

Nitrogen Catabolite Repression (NCR) allows the adaptation of yeast cells to the quality of nitrogen supply by inhibiting the transcription of genes encoding proteins involved in transport and degradation of nonpreferred nitrogen sources. In cells using ammonium or glutamine, the GATA transcription factor Gln3 is sequestered in the cytoplasm by Ure2 whereas it enters the nucleus after a shift to a nonpreferred nitrogen source like proline or upon addition of rapamycin, the TOR complex inhibitor. Recently, the Npr1 kinase and the Rsp5, Bul1/2 ubiquitin ligase complex were reported to have antagonistic roles in the nuclear import and Gln3-mediated activation. The Npr1 kinase controls the activity of various permeases including transporters for nitrogen sources that stimulate NCR such as the Mep ammonium transport systems. Combining data from growth tests, Northern blot analysis and Gln3 immunolocalization, we show that the Npr1 kinase is not a direct negative regulator of Gln3-dependent transcription. The derepression of Gln3-activated genes in ammonium-grown npr1 cells results from the reduced uptake of the nitrogen-repressing compound because NCR could be restored in npr1 cells by repairing ammonium-uptake defects through different means. Finally, we show that the impairment of the ubiquitin ligase complex does not prevent induction of NCR genes under nonpreferred nitrogen conditions. The apparent Rsp5-, Bul1/2-dependent Gln3 activation keeps to the cellular status, as it is only observed in cells having left the balanced phase of exponential growth.
Nuclear Spirals as Feeding Channels to the Supermassive Black Hole: The Case of the Galaxy NGC 6951

We report the discovery of gas streaming motions along nuclear spiral arms toward the LINER nucleus of the galaxy NGC 6951. The observations, obtained using the GMOS integral field spectrograph on the Gemini North telescope, yielded maps of the flux distributions and gas kinematics in the Hα, [N II] λ6584, and [S II] λλ6717, 6731 emission lines of the inner 7′′ × 15′′ of the galaxy. This region includes a circumnuclear star-forming ring with a radius of ~500 pc, a nuclear spiral inside the ring, and the LINER nucleus. The kinematics of the ionized gas is dominated by rotation, but subtraction of a kinematic model of a rotating exponential disk reveals deviations from circular rotation within the nuclear ring that can be attributed to (1) streaming motions along the nuclear spiral arms and (2) a bipolar outflow that seems to be associated with a nuclear jet. On the basis of the observed streaming velocities and geometry of the spiral arms, we estimate a mass inflow rate of ionized gas of ≈3 × 10-4 M☉ yr-1, which is on the order of the accretion rate necessary to power the LINER nucleus of NGC 6951. Similar streaming motions toward the nucleus of another galaxy with a LINER nucleus, NGC 1097, have been reported by our group in a previous paper. Taken together, these results support a scenario in which nuclear spirals are channels through which matter is transferred from galactic scales to the nuclear region to feed the supermassive black hole.
L-Arginine normalizes coronary vasomotion in long-term smokers.

BACKGROUND Noninvasive measurements of myocardial blood flow (MBF) with PET revealed an abnormal coronary vasomotor response to cold pressor test in healthy long-term smokers. If coronary endothelial dysfunction accounted for this abnormality, we hypothesized that it could be reversed by L-arginine as the substrate for NO synthase. METHODS AND RESULTS MBF was quantified with 13N-labeled ammonia and PET in 11 healthy smokers (age, 45+/-10 years; 27+/-10 years of smoking) and in 12 age-matched nonsmokers on 2 separate days. On day 1, MBF was measured at rest and, after intravenous L-arginine, during cold pressor test. On day 2, MBF was measured during cold pressor test and then at rest during L-arginine. Baseline rate-pressure product (RPP) (6559+/-1590 versus 7144+/-1157 bpmxmm Hg) and MBF (0.65+/-0.14 versus 0.73+/-0.13 mL x g-1 x min-1) were similar in nonsmokers and smokers. Cold pressor test increased RPP similarly in both groups (53+/-26% versus 46+/-26%), whereas MBF increased in nonsmokers (to 0.93+/-0.25 mL x g-1 x min-1; P<0.05) but not in smokers (0.80+/-0.16 mL x g-1 x min-1). The percent MBF increase differed between nonsmokers and smokers (44+/-25% versus 11+/-14%; P=0.0017). However, after L-arginine, the magnitude of MBF response to cold pressor test no longer differed between groups (48+/-36% versus 48+/-28%), whereas RPP again increased similarly in the 2 groups (59+/-30% versus 44+/-16%). L-Arginine had no effect on resting MBF in smokers or nonsmokers. CONCLUSIONS Our findings implicate the coronary endothelium as the major site of the abnormal vasomotor response in long-term smokers. Cold pressor test combined with PET imaging may allow the noninvasive identification of coronary endothelial dysfunction in humans.
Correction of a splice-site mutation in the beta-globin gene stimulated by triplex-forming peptide nucleic acids

Splice-site mutations in the beta-globin gene can lead to aberrant transcripts and decreased functional beta-globin, causing beta-thalassemia. Triplex-forming DNA oligonucleotides (TFOs) and peptide nucleic acids (PNAs) have been shown to stimulate recombination in reporter gene loci in mammalian cells via site-specific binding and creation of altered helical structures that provoke DNA repair. We have designed a series of triplex-forming PNAs that can specifically bind to sequences in the human beta-globin gene. We demonstrate here that these PNAs, when cotransfected with recombinatory donor DNA fragments, can promote single base-pair modification at the start of the second intron of the beta-globin gene, the site of a common thalassemia-associated mutation. This single base pair change was detected by the restoration of proper splicing of transcripts produced from a green fluorescent protein-beta-globin fusion gene. The ability of these PNAs to induce recombination was dependent on dose, sequence, cell-cycle stage, and the presence of a homologous donor DNA molecule. Enhanced recombination, with frequencies up to 0.4%, was observed with use of the lysomotropic agent chloroquine. Finally, we demonstrate that these PNAs were effective in stimulating the modification of the endogenous beta-globin locus in human cells, including primary hematopoietic progenitor cells. This work suggests that PNAs can be effective tools to induce heritable, site-specific modification of disease-related genes in human cells.
Calorific Value and Chemical Properties in Juvenile and Mature Wood of Thermally-Modified Eucalyptus Grandis

During thermal modifi cation, timber is exposed to temperatures approaching 200 oC for several hours and wood properties change. This study was aimed at evaluating the calorifi c value and chemical properties of juvenile and mature wood of thermally modifi ed Eucalyptus grandis. Boards were taken from 30-year-old E. grandis trees and thermally modifi ed at 180oC. Samples of the untreated and thermally modifi ed wood were transformed into chips and then processed into sawdust for the energy and chemical characterization of juvenile and mature wood. The results show that the thermal modifi cation causes: (1) signifi cant increases of 3.7 % and 6.8 %, respectively, in the net calorifi c value of juvenile and mature wood; (2) signifi cant increases of 24.8 % and 47.6 %, respectively, in the extractive content of juvenile and mature wood; (3) signifi cant decreases of 4.2 % and 8.3 % in the respective holocellulose content; and (4) signifi cant decreases in the galactose, xylose and glucose contents. It was demonstrated that the infl uence of thermal treatment was lower in juvenile wood than in mature wood.
Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization

Laccases have been used for the decolorization and detoxification of synthetic dyes due to their ability to oxidize a wide variety of dyes with water as the sole byproduct. A putative laccase gene (LacTT) from Thermus thermophilus SG0.5JP17-16 was screened using the genome mining approach, and it was highly expressed in Pichia pastoris, yielding a high laccase activity of 6130 U/L in a 10-L fermentor. The LacTT open reading frame encoded a protein of 466 amino acid residues with four putative Cu-binding regions. The optimal pH of the recombinant LacTT was 4.5, 6.0, 7.5 and 8.0 with 2,2'-azino-bis(3-ethylbenzothazoline-6-sulfonic acid) (ABTS), syringaldazine (SGZ), guaiacol, and 2,6-dimethoxyphenol (2,6-DMP) as the substrate, respectively. The optimal temperature of LacTT was 90°C with guaiacol as the substrate. LacTT was highly stable at pH 4.0–11.0 and thermostable at 40°C–90°C, confirming that it is a pH-stable and thermostable laccase. Furthermore, LacTT also exhibited high tolerance to halides such as NaCl, NaBr and NaF, and decolorized 100%, 94%, 94% and 73% of Congo Red, Reactive Black B and Reactive Black WNN, and Remazol Brilliant Blue R, respectively. Interestingly, addition of high concentration of NaCl increased the RBBR decolorization efficiency of LacTT. These results suggest that LacTT is a good candidate for industrial applications such as dyestuff processing and degradation of dyes in textile wastewaters.
A role for haptics in mobile interaction: initial design using a handheld tactile display prototype

Mobile interaction can potentially be enhanced with well-designed haptic control and display. However, advances have been limited by a vicious cycle whereby inadequate haptic technology obstructs inception of vitalizing applications. We present the first stages of a systematic design effort to break that cycle, beginning with specific usage scenarios and a new handheld display platform based on lateral skin stretch. Results of a perceptual device characterization inform mappings between device capabilities and specific roles in mobile interaction, and the next step of hardware re-engineering.
Influence of growth rate on susceptibility to antimicrobial agents: modification of the cell envelope and batch and continuous culture studies

Those interested in antimicrobial agents and chemotherapy culture microorganisms for a variety of tests. These include susceptibility assessments, bioassays, studies of the interactions between antibiotics and host defenses, and in vitro stimulation of in vivo conditions for purposes of extrapolation. The specific physiological state of the organisms used, especially surface properties, greatly influences the outcome of all such tests.
Genetic differentiation in Eurasian populations of the postfire ascomycete Daldinia loculata

The genetic population structure of the postfire ascomycete Daldinia loculata was studied to test for differentiation on a continental scale. Ninety‐six samples of spore families, each comprising mycelia from six to 10 spores originating from single perithecia, were sampled from one Russian and six Fennoscandian forest sites. Allelic distribution was assayed for six nuclear gene loci by restriction enzyme analyses of polymerase chain reaction (PCR)‐amplified gene fragments. In addition, the full sequence of the gene fragment was analysed for a subset of haploid single‐ascospore isolates in a multiallelic approach. A third data set was generated by using arbitrary‐primed PCR with the core sequence of the phage M13 as primer. Although there was a reduction in heterozygosity in the total population from what would have been expected at random mating, the levels of genetic differentiation among the Eurasian subpopulations of D. loculata were low. All subpopulations were found to be in Hardy–Weinberg equilibrium and gametic equilibrium was observed between all investigated nuclear gene loci. The results obtained by the different markers were consistent; we confirmed low levels of genetic differentiation among the Eurasian subpopulations of D. loculata. The differentiation did not increase with distance; the Russian subpopulation, sampled more than 7000 km from the Fennoscandian subpopulations, was only moderately differentiated from the others (FST = 0.00–0.14). In contrast, one of the Swedish populations was the most highly differentiated from the others, with FST and GST values of 0.10–0.16. The results suggest that D. loculata consists of a long‐lived backround Eurasian population of latent mycelia in nonburned forests, established by sexual ascospores dispersed from scattered burned forest sites. Local differentiation is probably due to founder effects of populations in areas with low fire frequency. A tentative life cycle of D. loculata is presented.
Morbidity and Mortality Pattern of Childhood Illnesses Seen at the Children Emergency Unit of Federal Medical Center, Asaba, Nigeria

Background: Morbidity and mortality from childhood illnesses has remained a major point of interest globally. Malaria, respiratory tract infection and diarrheal diseases are the leading causes of childhood morbidity and mortality. Aim: The aim of the following study is to determine the pattern of morbidity and mortality of children seen at the children emergency room of a tertiary hospital, Federal Medical Center in Asaba, Nigeria. Subjects and Methods: A descriptive study of post-neonatal childhood admissions over a 5 year period (January 2007-December 2011) was undertaken. Information obtained included age, gender, diagnosis, month of admission and outcome. Descriptive and inferential statistics were used to analyze the demographic characteristics of the patients and associations with outcome variables. Results: A total of 3,830 children with aged ranges between 1 month and 180 months were admitted within this period of study from January 2007 to December 2011: 2,189 males (57.2%, 2189/3830) and 1,641 (42.8%, 1641/3830) females. More than 70% (2912/3830) were under 5 years of age. The common indications for admission were malaria (30.3%, 1159/3830), diarrheal diseases (20.4%, 780/3830), respiratory tract infections (RTIs) (19.0%, 728/3830) and sepsis (4.4%, 168/3830). There were 221 (5.8%, 221/3830) deaths and more deaths were recorded in children <12 months of age, P < 0.01. Major causes of death were complicated malaria (24.4%, 54/221), sepsis (19.9%, 44/221), diarrheal diseases (18.1%, 40/221) and RTIs (7.7%, 17/221). Conclusion: Preventable infections are the major causes of morbidity and mortality in CHER and children <5 years of age are commonly affected.
Therapy for Specific Problems Therapy for Specific Problems : Youth Tobacco Cessation

Theory of mind is said to be uniquely human. Is this statement justified? Thirty years of research on a variety of species has produced differences in opinion, from unequivocal positive evidence to no evidence at all for mental attribution in animals. Our review concludes that animals are excellent ethologists, but on the whole, poor psychologists. Those studies that we believe present a good case for mental attribution all possess high ecological validity, including studies on food competition by chimpanzees and cache-protection strategies by corvids. Even though the current focus of research on prediction rather than explanation may be misplaced, we believe the field is now in a strong position to discover what animals really know about their fellow beings, be it based on simple associations, behavior reading, mind reading, or something else. 87 A nn u. R ev . P sy ch ol . 2 00 9. 60 :8 711 3. D ow nl oa de d fr om a rj ou rn al s. an nu al re vi ew s. or g by I N D IA N A U N IV E R SI T Y B lo om in gt on o n 10 /0 9/ 09 . F or p er so na l u se o nl y. ANRV364-PS60-04 ARI 27 October 2008 16:11
Chemokines in Oral Inflammatory Diseases: Apical Periodontitis and Periodontal Disease

The inflammatory oral diseases are characterized by the persistent migration of polymorphonuclear leukocytes, monocytes, lymphocytes, plasma and mast cells, and osteoblasts and osteoclasts. In the last decade, there has been a great interest in the mediators responsible for the selective recruitment and activation of these cell types at inflammatory sites. Of these mediators, the chemokines have received particular attention in recent years. Chemokine messages are decoded by specific receptors that initiate signal transduction events, leading to a multitude of cellular responses, including chemotaxis and activation of inflammatory and bone cells. However, little is known about their role in the pathogenesis of inflammatory oral diseases. The purpose of this review is to summarize the findings regarding the role of chemokines in periapical and periodontal tissue inflammation, and the integration, into experimental models, of the information about the role of chemokines in human diseases.
Clinical effectiveness of online computerised cognitive–behavioural therapy without support for depression in primary care: randomised trial

Background Computerised cognitive–behavioural therapy (CCBT) might offer a solution to the current undertreatment of depression. Aims To determine the clinical effectiveness of online, unsupported CCBT for depression in primary care. Method Three hundred and three people with depression were randomly allocated to one of three groups: Colour Your Life; treatment as usual (TAU) by a general practitioner; or Colour Your Life and TAU combined. Colour Your Life is an online, multimedia, interactive CCBT programme. No assistance was offered. We had a 6-month follow-up period. Results No significant differences in outcome between the three interventions were found in the intention-to-treat and per protocol analyses. Conclusions Online, unsupported CCBT did not outperform usual care, and the combination of both did not have additional effects. Decrease in depressive symptoms in people with moderate to severe depression was moderate in all three interventions. Online CCBT without support is not beneficial for all individuals with depression.
Replication of mycoplasma virus L51. VII. Effect of chloramphenicol on the synthesis of DNA replicative intermediates

Chloramphenicol affects several steps in the DNA replication of mycoplasma virus L51, a noncytocidal, naked, bullet-shaped virion containing circular single-stranded (SS) DNA of 1.5 X 10(6) daltons (4.5 kilobases). In the presence of chloramphenicol, adsorption was normal and parental SS DNA was converted to double-stranded replicative forms (RF), but subsequent RF leads to RF replication was inhibited. Chloramphenicol added late in infection, when most viral nascent DNA is in progeny SS molecules, inhibited SS synthesis, but nascent RF molecules were formed. However, a chase experiment showed that these RF molecules could not be converted to SS DNA. Therefore, viral RF molecules made in the presence of chloramphenicol are not functional as SS DNA precursors.
Robust output prediction of differential — Algebraic systems — Application to drinking water distribution system

The paper presents the recursive robust output variable prediction algorithm, applicable for systems described in the form of nonlinear algebraic-differential equations. The algorithm bases on the uncertainty interval description, the system model, and the measurements. To improve the algorithm efficiency, nonlinear system models are linearised along the nominal trajectory. The effectiveness of the algorithm is demonstrated on the model of the Chojnice drinking water supply system (DWDS).