Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Group 1 versus Group 2 Carbapenems on the Susceptibility of Acinetobacter baumannii to Carbapenems: A Before and After Intervention Study of Carbapenem-Use Stewardship

Objective Antimicrobial stewardship programs have been proposed for reducing bacterial resistance in the hospital environment. The purpose of this study was to investigate the impact of a carbapenem-use stewardship program on the susceptibility of Acinetobacter baumannii to Group 2 carbapenems. Methods A before and after intervention study was conducted at a university hospital from September 2008 to February 2013. Three study periods were defined: Phase I, pre-intervention (months 1–18); Phase II, a postintervention period during which ertapenem use was mandated but carbapenem use was not restricted (months 19–36); and Phase III, a postintervention period during which Group 2 carbapenem use was restricted (months 37–54). Results During the study period, intervention resulted in diminished consumption of Group 2 carbapenems (antimicrobial use density (AUD): 21.3±6.0 in Phase I, 18.8±6.0 in Phase II, 16.1±4.4 in Phase III; P = 0.028) and increased consumption of ertapenem (AUD: 2.7±1.7 in Phase I, 7.2±4.5 in Phase II, 9.1±5.3 in Phase III; P<0.001). The use of autoregressive-error models showed that in contrast with ertapenem use, the use of Group 2 carbapenem during the previous one month was positively and significantly associated with a subsequent increase in the proportion of carbapenem-resistant A. baumannii (CRAB) (P = 0.031). Conclusions Implementing a carbapenem-use stewardship program featuring the preferential use of ertapenem for treating appropriate indications of infection resulted in reduced use of Group 2 carbapenems and had a positive impact on the susceptibility of A. baumannii to carbapenems. This approach could be integrated into CRAB-control strategies in hospitals.
Experimental Validation of Dual H-Bridge Current Flow Controllers for Meshed HVdc Grids

The current-carrying capability of dc lines is limited by their thermal and electric stress limits. Thus, the line current must be maintained within the permissible operational region to protect the lines from damage. In a dense dc grid, control over each line current cannot be achieved without including additional control devices. In this paper, a dual H-bridge current flow controller (2B-CFC) is used to manage the dc grid line power flow by providing dc voltage compensation in series with dc lines. A centralized hierarchical control system is proposed to coordinate the operation between multiple CFCs. A novel voltage-sharing control scheme is demonstrated. It is shown that such a scheme reduces the workload on a single CFC by sharing the required control voltage between multiple CFCs, and, in addition, can be used to avoid control conflicts among active CFCs during communication failure. An experimental platform consisting of a three-terminal dc grid and small-scale 2B-CFC prototypes has been developed to validate the concepts. For completeness, the CFC performance has been analyzed for overload conditions and when no communication exists. Small-scale dc circuit breakers have been developed to study the CFC performance under a pole-to-pole fault.
Development of Ash Dieback in South-Eastern Germany and the Increasing Occurrence of Secondary Pathogens

Since its first identification in Poland in 2006, the ascomycete Hymenoscyphus fraxineus has caused massive dieback of Fraxinus excelsior in the countries of eastern, northern and central Europe. This work shows the development, expansion, and severity of the disease in south-eastern Germany for a period of four years, starting in 2010. Differences between habitats, as well as age classes have been captured. The presence and the amount of potentially resistant trees were proven over the years, to determine how high the resistance level might be. Typical disease symptoms are the wilting of leaves, necrotic lesions in the bark and reddish discolorations of branches and stems. In addition, stem necroses also appear by infection with species of Armillaria. Therefore, special attention has been given to Armillaria species in affected ash stands but also to other secondary pathogens, like ash bark beetles. It is shown that breeding galleries of Hylesinus fraxini are only found in trees that have recently died and thus Hylesinus fraxini is still acting as a secondary opportunistic pathogen. In contrast, Armillaria spp. can be considered as serious pathogens of weakened ash trees. In different ash stands, typical symptoms of infection can be found. A relationship between stem base necrotic lesions and vitality was examined. It is shown that necrotic lesions severely contribute to accelerating the mortality of ash trees. In addition to the high infection pressure by H. fraxineus, the high inoculum of Armillaria in the soil facilitates further infections and, thus, likewise endangers the survival of potentially resistant trees. In the following years, forest conversion and seed harvest in affected ash stands will have to be urgently considered to avoid tree gaps on a large scale. Furthermore, infection assays of potentially resistant trees with ensuing breeding programmes should be initially started for the conservation of this ecologically and economically important tree species.
Chemical carcinogens and antigens induce immune suppression via Langerhans' cell depletion

The ability of the chemical carcinogen dimethylbenz(a)anthracene (DMBA) to deplete Langerhans' cells (LC) from murine skin is crucial to the development of antigen‐specific suppression. This depletion is a consequence of the LC recognizing the DMBA as antigenic and migrating to the draining lymph nodes to attempt to elicit T‐cell activation. This depletion also occurred following exposure to high doses of the contact sensitizers 2,4‐dinitrofluorobenzene (DNFB), 2,4,6‐trinitrochlorobenzene (TNCB) and fluorescein isothiocyanate (FITC). However, LC depletion was not significant at lower doses, even though these doses were sufficient to induce strong contact sensitivity responses. Application of the contact sensitizer, DNFB, through skin depleted of LC (by pretreatment with either the carcinogen DMBA or the antigen TNCB) failed to induce contact sensitivity. This immune non‐responsiveness was antigen specific, and could be transferred by spleen cells to naive mice, which were unable to respond to DNFB. Mouse skin treated with doses of TNCB, that did not cause LC depletion but still induced a normal contact hypersensitivity, retained its ability to initiate a normal immune response to DNFB. Together these findings demonstrate that carcinogens share some properties with antigens as they both cause LC depletion and interact with the immune system. Furthermore, it is this LC depletion, rather than carcinogen treatment, that is a critical factor which leaves the skin immunologically compromised and favours the induction of antigen‐specific suppression.
Mammalian sperm molecules that are potentially important in interaction with female genital tract and egg vestments

Fertilisation is a highly programmed process by which two radically different cells, sperm and egg, unite to form a zygote, a cell with somatic chromosome numbers. Development of the zygote begins immediately after sperm and egg haploid pronuclei come together, pooling their chromosomes to form a single diploid nucleus with the parental genes. Mammalian fertilisation is the net result of a complex set of molecular events which allow the capacitated spermatozoa to recognise and bind to the egg's extracellular coat, the zona pellucida (ZP), undergo the acrosome reaction, and fuse with the egg plasma membrane. Sperm-zona (egg) interaction leading to fertilisation is a species-specific carbohydrate-mediated event which depends on glycan-recognising proteins (glycosyltransferases/glycosidases/lectin-like molecules) on sperm plasma membrane (receptors) and their complementary glycan units (ligands) on ZP. The receptor-ligand interaction event initiates a signal transduction pathway resulting in the exocytosis of acrosomal contents. The hydrolytic action of the sperm glycohydrolases and proteases released at the site of sperm-egg interaction, along with the enhanced thrust generated by the hyperactivated beat pattern of the bound spermatozoon, are important factors regulating the penetration of egg investments. This review focuses on sperm molecules believed to be important for the interaction with the female genital tract, passage through cumulus oophorus and attachment to ZP, induction of the acrosome reaction, secondary binding events, and passage through the ZP. An understanding of the expression and modifications of molecules thought to be important in multiple events leading to fertilisation will allow new strategies to block these modifications and alter sperm function.
Correlation between Subjective Nasal Patency and Intranasal Airflow Distribution

Objectives (1) Analyze the relationship between intranasal airflow distribution and subjective nasal patency in healthy and nasal airway obstruction (NAO) cohorts using computational fluid dynamics (CFD). (2) Determine whether intranasal airflow distribution is an important objective measure of airflow sensation that should be considered in future NAO virtual surgery planning. Study Design Cross-sectional. Setting Academic tertiary medical center and academic dental clinic. Subjects and Methods Three-dimensional models of nasal anatomy were created based on computed tomography scans of 15 patients with NAO and 15 healthy subjects and used to run CFD simulations of nasal airflow and mucosal cooling. Subjective nasal patency was quantified with a visual analog scale (VAS) and the Nasal Obstruction Symptom Evaluation (NOSE). Regional distribution of nasal airflow (inferior, middle, and superior) was quantified in coronal cross sections in the narrowest nasal cavity. The Pearson correlation coefficient was used to quantify the correlation between subjective scores and regional airflows. Results Healthy subjects had significantly higher middle airflow than patients with NAO. Subjective nasal patency had no correlation with inferior and superior airflows but a high correlation with middle airflow (|r| = 0.64 and |r| = 0.76 for VAS and NOSE, respectively). Anterior septal deviations tended to shift airflow inferiorly, reducing middle airflow and reducing mucosal cooling in some patients with NAO. Conclusion Reduced middle airflow correlates with the sensation of nasal obstruction, possibly due to a reduction in mucosal cooling in this region. Further research is needed to elucidate the role of intranasal airflow distribution in the sensation of nasal airflow.
Money, schooling, and health: Mechanisms and causal evidence

An association between higher educational attainment and better health status has been repeatedly reported in the literature. Similarly, thousands of studies have found a relationship between higher income and better health. However, whether these repeated observations amount to causality remains a challenge, not least because of the practical limitations of randomizing people to receive different amounts of money or schooling. In this essay, we review the potential causal mechanisms linking schooling and income to health, and discuss the twin challenges to causal inference in observational studies, in other words, reverse causation and omitted variable bias. We provide a survey of the empirical attempts to identify the causal effects of schooling and income on health, including natural experiments. There is evidence to suggest that schooling is causally related to improvements in health outcomes. Evidence also suggests that raising the incomes of the poor leads to improvement in their health outcomes. Much remains unknown beyond these crude findings, however; for example, what type of education matters for health, or whether there is a difference between the health impacts of temporary income shocks versus changes in long‐term income.
Phase diagram of a semiflexible polymer chain in a θ solvent: Application to protein folding

We consider a lattice model of a semiflexible homopolymer chain in a bad solvent. Beside the temperature T, this model is described by (i) a curvature energy eh, representing the stiffness of the chain; (ii) a nearest‐neighbor attractive energy ev, representing the solvent; and (iii) the monomer density ρ=N/Ω, where N and Ω denote, respectively, the number of monomers and the number of lattice sites. This model is a simplified view of the protein folding problem, which encompasses the geometrical competition between secondary structures (the curvature term modelling helix formation) and the global compactness (modeled here by the attractive energy), but contains no side chain information. By allowing the monomer density ρ to depart from unity one has made a first (albeit naive) step to include the role of the water. In previous analytical studies, we considered only the (fully compact) case ρ=1, and found a first order freezing transition towards a crystalline ground state (also called the native state in.
Dynamic ADTs: a "don't ask, don't tell" policy for data abstraction

We outline an approach to abstract data types (ADTs) that allows an object of the type specified by the ADT to take on one of many possible representations. A dynamic abstract data type (DADT) is dual to dynamic algorithm selection and facilitates profiling of data in conjunction with the profiling of code. It also permits a programmer to delay or ignore details pertaining to data representation and enhance the efficiency of some algorithms by changing representations at run time without writing code extraneous to the algorithm itself. Additionally, we demonstrate that run time optimization of data objects is possible and allows for acceptable performance compared to traditional ADTs. An implementation is presented in Common Lisp.
Oscillation Criteria for Second‐Order Nonlinear Dynamic Equations On Time Scales

By means of generalized Riccati transformation techniques and generalized exponential functions, some oscillation criteria are given for the nonlinear dynamic equation (p(t)xΔ(t))Δ+q(t)(f o xσ)=0 on time scales. The results are also applied to linear and nonlinear dynamic equations with damping, and some sufficient conditions are obtained for the oscillation of all solutions.
Therapeutic Cardiac‐Targeted Delivery of miR‐1 Reverses Pressure Overload–Induced Cardiac Hypertrophy and Attenuates Pathological Remodeling

Background MicroRNAs (miRNAs) play a key role in the development of heart failure, and recent studies have shown that the muscle‐specific miR‐1 is a key regulator of cardiac hypertrophy. We tested the hypothesis that chronic restoration of miR‐1 gene expression in vivo will regress hypertrophy and protect against adverse cardiac remodeling induced by pressure overload. Methods and Results Cardiac hypertrophy was induced by left ventricular pressure overload in male Sprague‐Dawley rats subjected to ascending aortic stenosis. When the hypertrophy was established at 2 weeks after surgery, the animals were randomized to receive either an adeno‐associated virus expressing miR‐1 (AAV9.miR‐1) or green fluorescent protein (GFP) as control (AAV9.GFP) via a single‐bolus tail‐vein injection. Administration of miR‐1 regressed cardiac hypertrophy (left ventricular posterior wall thickness,; 2.32±0.08 versus 2.75±0.07 mm, P<0.001) and (left ventricular septum wall thickness, 2.23±0.06 versus 2.54±0.10 mm, P<0.05) and halted the disease progression compared with control‐treated animals, as assessed by echocardiography (fractional shortening, 37.60±5.01% versus 70.68±2.93%, P<0.05) and hemodynamic analyses (end‐systolic pressure volume relationship/effective arterial elastance, 1.87±0.46 versus 0.96±0.38, P<0.05) after 7 weeks of treatment. Additionally, miR‐1 replacement therapy lead to a marked reduction of myocardial fibrosis, an improvement in calcium handling, inhibition of apoptosis, and inactivation of the mitogen‐activated protein kinase signaling pathways, suggesting a favorable effect on preventing the maladaptive ventricular remodeling. We also identified and validated a novel bona fide target of miR‐1, Fibullin‐2 (Fbln2), a secreted protein implicated in extracellular matrix remodeling. Conclusions Taken together, our findings suggest that restoration of miR‐1 gene expression is a potential novel therapeutic strategy to reverse pressure‐induced cardiac hypertrophy and prevent maladaptive cardiac remodeling.
3-D Stacked Package Technology and Trends

The need to integrate more device technology in a given board space for handheld applications such as mobile phones has driven the adoption of innovative packages which stack such devices in the vertical or third dimension (3D). Stacking of device chips in small and thin fine-pitch ball grid array packages has evolved into the stacking of packages themselves to achieve the same end. The advantage of stacking packages rather than device chips is that packages can be fully tested good prior to stacking. There are two primary ways to stack packages to achieve such vertical integration: package-on-package (PoP) and package-in-package (PiP). Innovative variations of PoP and PiP are being developed to address specific packaging needs and market trends. This paper will detail some of the key technology supporting PoP and PiP packages currently in production and the development of new variations of such packages to address future trends.
Searches for rare or forbidden semileptonic charm decays

We present searches for rare or forbidden charm decays of the form X(c)(+) -> h(+/-)l(+/-)l((l)+), where X(c)(+) is a charm hadron (D(+), D(s)(+), or A(c)(+)), h +/- is a pion, kaon, or proton, and l((l)+/-) is an electron or muon. The analysis is based on 384 fb(-1) of e(+)e(-) annihilation data collected at or close to the gamma(4S) resonance with the BABAR detector at the SLAC National Accelerator Laboratory. No significant signal is observed for any of the 35 decay modes that are investigated. We establish 90% confidence-level upper limits on the branching fractions between 1 x 10(-6) and 44 x 10(-6) depending on the channel. In most cases, these results represent either the first limits or significant improvements on existing limits for the decay modes studied.
A Sustained Dietary Change Increases Epigenetic Variation in Isogenic Mice

Epigenetic changes can be induced by adverse environmental exposures, such as nutritional imbalance, but little is known about the nature or extent of these changes. Here we have explored the epigenomic effects of a sustained nutritional change, excess dietary methyl donors, by assessing genomic CpG methylation patterns in isogenic mice exposed for one or six generations. We find stochastic variation in methylation levels at many loci; exposure to methyl donors increases the magnitude of this variation and the number of variable loci. Several gene ontology categories are significantly overrepresented in genes proximal to these methylation-variable loci, suggesting that certain pathways are susceptible to environmental influence on their epigenetic states. Long-term exposure to the diet (six generations) results in a larger number of loci exhibiting epigenetic variability, suggesting that some of the induced changes are heritable. This finding presents the possibility that epigenetic variation within populations can be induced by environmental change, providing a vehicle for disease predisposition and possibly a substrate for natural selection.
Regrets Only! Online Stochastic Optimization under Time Constraints

This paper considers online stochastic optimization problems where time constraints severely limit the number of offline optimizations which can be performed at decision time and/or in between decisions. It proposes a novel approach which combines the salient features of the earlier approaches: the evaluation of every decision on all samples (expectatio0n) and the ability to avoid distributing the samples among decisions (consensus). The key idea underlying the novel algorithm is to approximate the regret of a decision d. The regret algorithm is evaluated on two fundamentally different applications: online packet scheduling in networks and online multiple vehicle routing with time windows. On both applications, it produces significant benefits over prior approaches.
Bending and compression tests for PA 2200 parts obtained using Selective Laser Sintering method

Craniofacial bone defects that produce morphological and functional disabilities can bring alteration of the quality of patient’s life. For this reason, the collaboration between the field of medicine with engineering, lead to the manufacture of custom implants from biocompatible materials. In the last years it was shown that Additive Manufacturing methods are very helpful to achieve customized medical implants. All the customized implants made of biocompatible materials have to be tested for mechanical properties, in order to have the optimal characteristics for the area were will be used. This paper presents the results for bending and compression tests for a biocompatible material, PA 2200. The samples were achieved using Selective Laser Sintering method and were obtained with different laser power, starting with 4 W. Maximum load, maximum stress, specific deformation and Young's modulus were analysed. The study showed that different mechanical properties can be obtained, depending on the laser power used. At bending tests, increasing values were obtained for the investigated parameters along with laser power increasing, but at compression tests a different trend was observed.
Muscle-derived neurotrophin-3 reduces injury-induced proprioceptive degeneration in neonatal mice.

During perinatal development, proprioceptive muscle afferents are quite sensitive to nerve injury. Here, we have used transgenic mice that overexpress neurotrophin-3 (NT-3) in skeletal muscle (myo/NT-3 mice) to explore whether NT-3 plays a neuroprotective role for perinatal muscle afferents following nerve injury. Measurements of NT-3 mRNA using RT-PCR revealed that levels of endogenous NT-3 mRNA in wild-type muscles remained constant during the first postnatal week following nerve crush or nerve section on postnatal day (PN) 1. In comparison, myo/NT-3 mice had significantly elevated levels of NT-3 mRNA that were maintained or increased following injury. To assess whether muscle-derived NT-3 could prevent injury-induced neuronal death, neuron survival in the DRG was analyzed in mice 5 days after sciatic nerve crush on PN3. Retrograde prelabeling of muscle afferents and parvalbumin immunocytochemistry both revealed that overexpression of NT-3 in muscle significantly reduced neuronal loss following injury. Similar neuroprotective effects of NT-3 were observed in wild-type mice injected with exogenous NT-3 in the gastrocnemius muscles. To test whether NT-3 could prevent muscle spindle degeneration, spindle number and morphology were assessed 3 weeks after sciatic nerve crush or section on PN1. No spindles were present in either wildtype or myo/NT-3 muscles after nerve section, demonstrating that NT-3 overexpression cannot maintain spindles following complete denervation. Moreover, NT-3 overexpression could not prevent moderate spindle loss in muscle and did not stimulate new spindle formation following nerve crush. Our results demonstrate that in addition to its early actions on sensory neuron generation and naturally occurring cell death, NT-3 has important neuroprotective effects on muscle afferents during postnatal development.