Miracle-dz/UniFAR-FTUs huggingface dataset:
Combined Contribution of Increased Intestinal Permeability and Inhibited Deglycosylation of Ginsenoside Rb1 in the Intestinal Tract to the Enhancement of Ginsenoside Rb1 Exposure in Diabetic Rats after Oral Administration

Panax ginseng is becoming a promising antidiabetic herbal medication. As the main active constituents of Panax ginseng, ginsenosides are well known, poorly absorbed chemicals. However, the pharmacokinetic behavior of ginsenosides under diabetic conditions is not fully understood. This study aimed to explore the alterations and potential mechanisms of pharmacokinetic behavior of ginsenoside Rb1 in diabetic rats compared with normal rats and rats fed a high-fat diet. Systemic exposure (area under the concentration-time curve extrapolated from zero to infinity) was significantly increased in diabetic rats after oral administration of Rb1. Oral bioavailability of Rb1 was significantly higher in diabetic rats (2.25%) compared with normal rats (0.90%) and rats fed a high-fat diet (0.78%). Further studies revealed that increased Rb1 exposure in diabetic rats may be mainly attributed to increased Rb1 absorption via the intestine and inhibited Rb1 deglycosylation by the intestinal microflora. Neither metabolic enzymes nor drug transporters displayed appreciable effects on Rb1 disposition. The transport of paracellular markers (fluorescein sodium and fluorescein isothiocyanate-dextran of 4 kDa) as well as Rb1 itself across the Caco-2 monolayer cultured with diabetic serum was promoted, demonstrating that increased paracellular permeability of the Caco-2 monolayer may benefit intestinal Rb1 absorption. In addition, Rb1 exposure was decreased in diabetic rats after Rb1 intravenous administration, which may result from increased Rb1 urinary excretion. In conclusion, Rb1 oral exposure was significantly increased under diabetic conditions, which is of positive significance to clinical treatment. The potential mechanism may be associated with the combined contribution of increased gut permeability and inhibited deglycosylation of ginsenoside Rb1 by intestinal microflora.
A simple byte-erasure method for improved impulse immunity in DSL

The data that is transmitted in DSL system is subject to corruption by impulse noise, i.e., noise bursts of high energy that interfere with the transmitted symbols. As DSL data rates increase the crosstalk mitigation techniques become more sophisticated, impulse noise limits service in terms of rate or delay. Because of the highly non-stationary nature of impulse noise, a combination of interleaving and Reed-Solomon coding is currently used to shield systems from noise burst. This paper presents a modified impulse noise protection algorithm that takes advantage of the improved performance of Reed-Solomon codes when the location of the impaired bytes is known. Without changing the structure of the encoder or the interleaver, it is shown that the delay, or equivalently the overhead due to forward error correction coding, can be reduced without compromising the immunity of the system to impulses. A DMT-VDSL system is used as a particular example of the improvement achieved using byte-erasure.
Design of a Knowledge-Based Report Generator

Knowledge-Based Report Generation is a technique for automatically generating natural language reports from computer databases. It is so named because it applies knowledge-based expert systems software to the problem of text generation. The first application of the technique, a system for generating natural language stock reports from a daily stock quotes database, is partially implemented. Three fundamental principles of the technique are its use of domain-specific semantic and linguistic knowledge, its use of macro-level semantic and linguistic constructs (such as whole messages, a phrasal lexicon, and a sentence-combining grammar), and its production system approach to knowledge representation.
Interpretation of English multiple wh-questions by Japanese speakers: a missing uninterpretable feature account

In recent work by Tsimpli (2003) and Tsimpli and Dimitrakopoulou (to appear) an explicit claim is made about the nature of end-state grammars in older second language (L2) learners: uninterpretable syntactic features that have not been selected during first language (L1) acquisition will not be available for L2 grammar construction. Interpretable syntactic features, on the other hand, remain available (as well as the computational procedures and principles of the language faculty), even those not selected by the L1. The present study investigates this hypothesis in relation to the acquisition of the uninterpretable feature that forces wh-movement in interrogatives in English. Nineteen L1 speakers of Japanese (a wh-in-situ language that lacks the movement-forcing feature) who are highly proficient speakers of English were asked to interpret bi-clausal multiple wh-questions in English (like Where did the professor say the students studied when? ). Their responses were compared with those of a native speaker control group. It is argued that the results are consistent with the unavailability of the uninterpretable feature. Two conclusions are drawn: first, that there is a critical period for the selection of uninterpretable syntactic features for the construction of mental grammars; second, that despite the observation of target-like performance by L1 Japanese speakers on English wh-interrogatives reported in a number of existing studies, caution is required in interpreting target-like performance as evidence that L2 speakers have the same underlying grammatical representations as native speakers.
Dectin-2 Recognition of House Dust Mite Triggers Cysteinyl Leukotriene Generation by Dendritic Cells1

House dust mites are a significant source of airborne allergen worldwide, but there is little understanding of how they so potently generate allergic inflammation. We found that extracts from the house dust mites Dermatophagoides farinae (Df) and Dermatophagoides pteronyssinus and from the mold Aspergillus fumigatus stimulated a rapid and robust production of cysteinyl leukotrienes (cys-LTs), proinflammatory lipid mediators, from mouse bone marrow-derived dendritic cells (BMDCs). Con A affinity chromatography of the Df extract revealed that the relevant ligand is a glycan(s), suggesting stimulation via a dendritic cell (DC) lectin receptor. Cys-LT production in BMDCs from wild-type mice was inhibited by spleen tyrosine kinase (Syk) inhibitors and was abolished in BMDCs from FcRγ−/− mice, implicating either Dectin-2 or DC immunoactivating receptor. Transfection of each receptor in bone marrow-derived mast cells revealed that only Dectin-2 mediates cys-LT production by Df, Dermatophagoides pteronyssinus, and Aspergillus fumigatus. Lentiviral knockdown of Dectin-2 in BMDCs attenuated Df extract-elicited cys-LT generation, thereby identifying Dectin-2 as the receptor. Lung CD11c+ cells, but not peritoneal or alveolar macrophages, also generated cys-LTs in response to Df. These findings place Dectin-2 among the C-type lectin receptors that activate arachidonic acid metabolism and identify the Dectin-2/FcRγ/Syk/cys-LT axis as a novel mechanism by which three potent indoor allergens may activate innate immune cells to promote allergic inflammation.
On the borders: adjusting to academic, social and cultural practices at an Australian university

Adjustment to university is challenging for students as they navigate a path through new academic, social and cultural practices. Some may feel on the borders, marginalised by their background. Issues such as adjustment to university life, independence, performance expectations, establishing friendships, technological competence, cultural capital, engaging with others and financial difficulties are addressed. Widening participation and the establishment of equitable access are worthy goals for higher education. This paper investigates cultural characteristics typical of universities, and of students from lower socio-economic backgrounds, and, implicitly, influences such as the schools, communities and families that have shaped these students. The paper reports on perceptions of first-year teacher education students at a university in Sydney, Australia, and explores ways of responding to potential cultural mismatches. It reveals their experiences of university life and sheds light on resources, services and cultural changes that could help in their adjustment and success.
Mild cognitive impairment in Parkinson’s disease: the challenge and the promise

This review addresses the literature surrounding Parkinson’s disease (PD) and mild cognitive impairment (MCI). It discusses the neuropsychological, pharmaceutical, and pathological overlap, the socioeconomic impact of PD and MCI, and the value of recognizing, understanding, and treating MCI in PD. It is concluded from this review that MCI in PD does exist and should be considered in clinical and research investigations. Due to the lack of accepted clinical criteria, an inclusive operating definition of MCI in PD is proposed. Research guidelines for studying the presence of MCI in PD and evaluating the efficacy of pharmaceutical interventions are also suggested.
ATM-deficient thymic lymphoma is associated with aberrant tcrd rearrangement and gene amplification

Ataxia telangiectasia mutated (ATM) deficiency predisposes humans and mice to T lineage lymphomas with recurrent chromosome 14 translocations involving the T cell receptor α/δ (Tcra/d) locus. Such translocations have been thought to result from aberrant repair of DNA double-strand breaks (DSBs) during Tcra locus V(D)J recombination, and to require the Tcra enhancer (Eα) for Tcra rearrangement or expression of the translocated oncogene. We now show that, in addition to the known chromosome 14 translocation, ATM-deficient mouse thymic lymphomas routinely contain a centromeric fragment of chromosome 14 that spans up to the 5′ boundary of the Tcra/d locus, at which position a 500-kb or larger region centromeric to Tcra/d is routinely amplified. In addition, they routinely contain a large deletion of the telomeric end of one copy of chromosome 12. In contrast to prior expectations, the recurrent translocations and amplifications involve V(D)J recombination–initiated breaks in the Tcrd locus, as opposed to the Tcra locus, and arise independently of the Eα. Overall, our studies reveal previously unexpected mechanisms that contribute to the oncogenic transformation of ATM-deficient T lineage cells.
Production of Live Piglets Following Cryopreservation of Embryos Derived from In Vitro-Matured Oocytes1

Abstract We have successfully produced healthy piglets following cryopreservation of embryos derived from oocytes matured and fertilized in vitro. The appropriate timing of cryopreservation pretreatment (removal of cytoplasmic lipid droplets [delipation] and vitrification) was initially determined using parthenogenetic embryos derived from in vitro-matured (IVM) oocytes. Viable embryos were obtained at the highest rate when embryos were delipated at the four- to eight-cell stages (Day 2 of embryo culture) and were vitrified approximately 15 h later (Day 3) by means of the minimum volume cooling method. After cryopreservation of embryos derived from oocytes matured and fertilized in vitro under the most appropriate conditions, 401 embryos were transferred to five recipient gilts, and the recipients all became pregnant. At autopsy of one of the recipients, which had received 47 embryos, eight fetuses (17.0%) were found. Three recipients each gave birth to two to four piglets (1.4%–6.0%). These results demonstrate that normal offspring can be produced from vitrified porcine embryos derived from IVM oocytes by a strategic combination of delipation and vitrification at the early cleavage stages. This approach has great potential in the reproduction of micromanipulated porcine embryos, such as cloned and sperm-injected embryos, produced from IVM oocytes.
Endothelium-Dependent Hyperpolarization to Acetylcholine in Carotid Artery of Guinea Pig: Role of Lipoxygenase

This study was designed to determine whether lipoxygenase-dependent metabolites of arachidonic acid are involved in the endothelium-dependent hyperpolarization of the guinea pig carotid artery. The membrane potential of vascular smooth muscle cells was measured with intracellular microelectrodes and potassium channels were studied on freshly isolated cells with the patch-clamp technique. Acetylcholine-induced hyperpolarizations were not affected by arachidonyl trifluoromethyl ketone (AACOCF3), quinacrine (phospholipase A2 inhibitors), or eicosatetraenoic acid (nonspecific inhibitor of lipoxygenase, cytochrome P450, and cyclooxygenase). In contrast, cinnamyl-3,4 dihydroxy-&agr;-cyanocinnamate (CDC) and AA861 (lipoxygenase inhibitors) as well as 1-(6-(17&bgr;-3-methoxyestra-1,3,5(10)-trien-17-yl)amino) hexyl)-1H-pyrrole-2,5-dione (U-73122) (phospholipase C inhibitor) produced a significant inhibition of the hyperpolarization. An opener of intermediate conductance calcium-activated potassium channels, 1-ethyl-2-benzamidazolinone (1-EBIO), induced a hyperpolarization that was unaffected by AACOCF3, CDC, AA861, or U-73122 but was inhibited by charybdotoxin. (±)12-hydroxy-eicosatetraenoic acid (12-HETE) and 12(S)-hydroperoxy-eicosatetraenoic acid (12(S)-HpETE) did not induce any significant changes in membrane potential. CDC inhibited the voltage-gated potassium current and increased the large conductance calcium-activated potassium current whereas AA861 inhibited both potassium currents. These results confirm that, in the isolated carotid artery of the guinea pig, stimulation of endothelial muscarinic receptors involves phospholipase C activation and indicate that the activation of phospholipase A2 and the release of lipoxygenase metabolites is unlikely to explain endothelium-dependent hyperpolarization.
Enteroendocrine localization of GLP-2 receptor expression in humans and rodents.

BACKGROUND & AIMS Glucagon-like peptide (GLP)-2, a product of the proglucagon gene, is expressed in enteroendocrine cells of the small and large intestine and is trophic to the gastrointestinal mucosa. GLP-2 also inhibits gastric acid secretion and emptying and up-regulates intestinal hexose transport. GLP-2 acts via binding to a single G protein-coupled GLP-2 receptor (GLP-2R), but the cellular targets for the diverse actions of GLP-2 remain unknown. METHODS GLP-2R expression in rodent and human tissues was examined using a combination of Northern blotting, reverse-transcription polymerase chain reaction (RT-PCR), and immunocytochemistry. RESULTS A single major GLP-2R messenger RNA transcript was detected by Northern blot analysis in rodent stomach, duodenum, jejunum, ileum, and colon, but not in rodent esophagus. GLP-2R expression was also detected by RT-PCR in RNA from the hypothalamus, brain stem, and lung. Immunocytochemical localization of human GLP-2R expression using specific antisera detected GLP-2R immunopositivity in subsets of endocrine cell populations in the epithelium of the stomach and both the small and large bowel. CONCLUSIONS These findings suggest that enteroendocrine-derived GLP-2 acts directly on endocrine cells to induce one or more downstream mediators of GLP-2 action in the gastrointestinal tract.
Comparison of Survival and Recurrence Pattern Between Two-Field and Three-Field Lymph Node Dissections for Upper Thoracic Esophageal Squamous Cell Carcinoma

Introduction: It is controversial to routinely perform three-field lymph node dissection in patients with upper thoracic esophageal carcinoma. The objective of this study was to compare survival and recurrence according to the extent of lymph node dissection in patients with upper thoracic esophageal squamous cell cancer. Methods: Between 1995 and 2007, 91 patients underwent R0 esophagectomy (with no residual tumor) for squamous cell carcinoma of the upper thoracic esophagus at our institution. Of these, 57 patients received three-field (cervical, mediastinal, and abdominal stations) lymph node dissection (3 FL group), whereas 34 received two-field (mediastinal and abdominal stations) lymph node dissection (2 FL group). We retrospectively compared the early and late postoperative outcomes between the two groups. Results: No differences were observed between the two groups with regard to age, gender, and pathologic stage. There was no in-hospital mortality in either group. The 5-year survival rate was 52% for the 2 FL group and 44% for the 3 FL group (p = 0.65). The disease-free 5-year survival rate was 39% for the 2 FL group and 38% for the 3 FL group (p = 0.97). The overall recurrence rate and the incidence of cervical nodal recurrence were not significantly different between the two groups. Conclusions: Our findings suggest that there was no survival benefit from the addition of cervical nodal dissection in patients with upper thoracic esophageal squamous cell carcinoma who had no evidence of cervical lymph node metastasis.
Beyond social ties: The impact of social capital on labour market outcomes for young Swedish people

This study makes use of a dataset which contains material relating to young Swedish people who have recently completed their studies and started working. It explores whether using social networks as such or using individuals’ resources which are accessible through social networks (social capital) provides relative advantages in the competition for better jobs. Interest in this topic stems from the recent development of sociological theories in this field. The results indicate that the use of social ties is a common way to find a job in the highly regulated Swedish labour market, but that informal recruitment methods per se provide no relative advantages in the competition for better jobs. On the other hand, given the same demographic characteristics, socioeconomic background and educational attainments, there is a positive association between resources embedded in an individual’s social network (social capital) and the quality of the jobs obtained.
Are Demographics Important for Forgiveness?

The current study primarily investigates the effect of age, gender, education, employment status, number of children, and number of years in marriage on forgiveness of first-married and remarried adults. When corrected for marital status (p < .005) and gender (p < .002), there is a significant main effect of number of children on forgiveness. There are also significant main effects of gender on forgiveness when corrected for education (p < .04), number of children (p < .003), and length of marriage (p < .01). Women's forgiveness is found to be significantly higher than men (p < .005, one-tailed, d = .20). Number of children (r = .092, p < .05) and educational attainment of women (r = .098, p < .05) also indicated a significant positive association with forgiveness.
Associations of Residential Long-Term Air Pollution Exposures and Satellite-Derived Greenness with Insulin Resistance in German Adolescents

Background: Epidemiological studies have identified associations between air pollution and green space access with type 2 diabetes in adults. However, it remains unclear to what extent associations with greenness are attributable to air pollution exposure. Objectives: We aimed to investigate associations between long-term exposure to air pollution and satellite-derived greenness with insulin resistance in adolescents. Methods: A total of 837 participants of two German birth cohorts (LISAplus and GINIplus) were included in the analysis. Generalized additive models were used to determine the association of individual satellite-derived greenness defined by the Normalized Difference Vegetation Index (NDVI), long-term air pollution exposure estimated by land-use regression (LUR) models with insulin resistance (HOMA-IR) in 15-year-old adolescents. Models were adjusted for study area, cohort, socioeconomic, and individual characteristics such as body mass index, physical activity, and smoking. Results: Increases of 2 SDs in nitrogen dioxide (NO2; 8.9 μg/m3) and particulate matter ≤ 10 μm in diameter (PM10; 6.7 μg/m3) were significantly associated with 11.4% (95% CI: 4.4, 18.9) and 11.4% (95% CI: 0.4, 23.7) higher HOMA-IR. A 2-SD increase in NDVI in a 1,000-m buffer (0.2 units) was significantly associated with a lower HOMA-IR (–7.4%; 95% CI: –13.3, –1.1). Associations tended to be stronger in adolescents who spent more time outside and in those with lower socioeconomic status. In combined models including both air pollution and greenness, only NO2 remained significantly associated with HOMA-IR, whereas effect estimates for all other exposures attenuated after adjustment for NO2. Conclusions: NO2, often considered as a marker of traffic, was independently associated with insulin resistance. The observed association between higher greenness exposure and lower HOMA-IR in adolescents might thus be attributable mainly to the lower co-exposure to traffic-related air pollution. Citation: Thiering E, Markevych I, Brüske I, Fuertes E, Kratzsch J, Sugiri D, Hoffmann B, von Berg A, Bauer CP, Koletzko S, Berdel D, Heinrich J. 2016. Associations of residential long-term air pollution exposures and satellite-derived greenness with insulin resistance in German adolescents. Environ Health Perspect 124:1291–1298; http://dx.doi. org/10.1289/ehp.1509967
A Positive Regulator of Nodule Organogenesis, NODULE INCEPTION, Acts as a Negative Regulator of Rhizobial Infection in Lotus japonicus1[W]

A transcription factor, known as a positive regulator of nodule organogenesis in root cortex, acts as a negative regulator of epidermal infection events by rhizobia. Legume-rhizobium symbiosis occurs in specialized root organs called nodules. To establish the symbiosis, two major genetically controlled events, rhizobial infection and organogenesis, must occur. For a successful symbiosis, it is essential that the two phenomena proceed simultaneously in different root tissues. Although several symbiotic genes have been identified during genetic screenings of nonsymbiotic mutants, most of the mutants harbor defects in both infection and organogenesis pathways, leading to experimental difficulty in investigating the molecular genetic relationships between the pathways. In this study, we isolated a novel nonnodulation mutant, daphne, in Lotus japonicus that shows complete loss of nodulation but a dramatically increased numbers of infection threads. Characterization of the locus responsible for these phenotypes revealed a chromosomal translocation upstream of NODULE INCEPTION (NIN) in daphne. Genetic analysis using a known nin mutant revealed that daphne is a novel nin mutant allele. Although the daphne mutant showed reduced induction of NIN after rhizobial infection, the spatial expression pattern of NIN in epidermal cells was broader than that in the wild type. Overexpression of NIN strongly suppressed hyperinfection in daphne, and daphne phenotypes were partially rescued by cortical expression of NIN. These observations suggested that the daphne mutation enhanced the role of NIN in the infection pathway due to a specific loss of the role of NIN in nodule organogenesis. Based on these results, we provide evidence that the bifunctional transcription factor NIN negatively regulates infection but positively regulates nodule organogenesis during the course of the symbiosis.