Miracle-dz/UniFAR-FTUs huggingface dataset:
Decoding the link between WWOX and p53 in aggressive breast cancer

ABSTRACT Basal-like breast cancer (BLBC) and triple-negative breast cancer (TNBC) are aggressive forms of human breast cancer with poor prognosis and limited treatment response. Molecular understanding of BLBC and TNBC biology is instrumental to improve detection and management of these deadly diseases. Tumor suppressors WW domain-containing oxidoreductase (WWOX) and TP53 are altered in BLBC and in TNBC. Nevertheless, the functional interplay between WWOX and p53 is poorly understood. In a recent study by Abdeen and colleagues, it has been demonstrated that WWOX loss drives BLBC formation via deregulating p53 functions. In this review, we highlight important signaling pathways regulated by WWOX and p53 that are related to estrogen receptor signaling, epithelial-to-mesenchymal transition, and genomic instability and how they impact BLBC and TNBC development.
Multi-factor analysis in language production: Sequential sampling models mimic and extend regression results

ABSTRACT For multi-factor analyses of response times, descriptive models (e.g., linear regression) arguably constitute the dominant approach in psycholinguistics. In contrast empirical cognitive models (e.g., sequential sampling models, SSMs) may fit fewer factors simultaneously, but decompose the data into several dependent variables (a multivariate result), offering more information to analyze. While SSMs are notably popular in the behavioural sciences, they are not significantly developed in language production research. To contribute to the development of this modelling in language, we (i) examine SSMs as a measurement modelling approach for spoken word activation dynamics, and (ii) formally compare SSMs to the default method, regression. SSMs model response activation or selection mechanisms in time, and calculate how they are affected by conditions, persons, and items. While regression procedures also model condition effects, it is only in respect to the mean RT, and little work has been previously done to compare these approaches. Through analyses of two language production experiments, we show that SSMs reproduce regression predictors, and further extend these effects through a multivariate decomposition (cognitive parameters). We also examine a combined regression-SSM approach that is hierarchical Bayesian, which can jointly model more conditions than classic SSMs, and importantly, achieve by-item modelling with other conditions. In this analysis, we found that spoken words principally differed from one another by their activation rates and production times, but not their thresholds to be activated.
HCI for Recommender Systems: the Past, the Present and the Future

How can you discover something new, that matches your interest? Recommender Systems have been studied since the 90ies. Their benefit comes from guiding a user through the density of the information jungle to useful knowledge clearings. Early research on recommender systems focuses on algorithms and their evaluation to improve recommendation accuracy using F-measures and other methodologies from signal-detection theory. Present research includes other aspects such as human factors that affect the user experience and interactive visualization techniques to support transparency of results and user control. In this paper, we analyze all publications on recommender systems from the scopus database, and particularly also papers with such an HCI focus. Based on an analysis of these papers, future topics for recommender systems research are identified, which include more advanced support for user control, adaptive interfaces, affective computing and applications in high risk domains.
Lipid Peroxidation, Protein Oxidation, Gelatinases, and Their Inhibitors in a Group of Adults with Obesity

Abstract The association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30–35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.
Effect of Prenatal Repair of Myelomeningocele on Urological Outcomes at School Age.

PURPOSE To investigate longer-term urological outcomes in patients enrolled in the Management of Myelomeningocele Study (MOMS). MATERIALS AND METHODS Women who participated in the original trial were asked for consent to their child being followed-up at 6 years or older in a single comprehensive study visit to a MOMS center. Participating children underwent urologic and radiologic procedures to provide objective evidence of current bladder functioning. Primary urologic outcome was defined as any: need for CIC, vesicostomy, urethral dilatation or augmentation cystoplasty. RESULTS 156 children were analyzed, mean age 7.4 years. 62% versus 87% in the prenatal and postnatal surgery groups respectively were placed on CIC (RR 0.71, 95% CI 0.58-0.86 p<0.001). Voiding status was significantly different between the groups (p= p<0.001); 24% in the prenatal group versus 4% in the postnatal group (RR 5.8, 95% CI 1.8-18.7) were reported to be voiding volitionally. Augmentation cystoplasty, vesicostomy, and urethral dilation did not differ between the two groups. Aside from a larger post residual urodynamic catheterization volume, there were no other statistical differences in video-urodynamic data or findings on renal/bladder ultrasound. CONCLUSIONS Prenatal closure of myelomeningocele resulted in less reported CIC at school-age. The mechanism for this is unclear. Although most children are in diapers or on CIC, parental reports showed children who underwent prenatal closure may be more likely to void volitionally than the postnatal group. Despite these findings, urologic outcomes alone should not be the sole impetus to perform in utero closure in children with spina bifida.
The role of trust in the social heuristics hypothesis

According to the social heuristics hypothesis, people intuitively cooperate or defect depending on which behavior is beneficial in their interactions. If cooperation is beneficial, people intuitively cooperate, but if defection is beneficial, they intuitively defect. However, deliberation promotes defection. Here, we tested two novel predictions regarding the role of trust in the social heuristics hypothesis. First, whether trust promotes intuitive cooperation. Second, whether preferring to think intuitively or deliberatively moderates the effect of trust on cooperation. In addition, we examined whether deciding intuitively promotes cooperation, compared to deciding deliberatively. To evaluate these predictions, we conducted a lab study in Colombia and an online study in the United Kingdom (N = 1,066; one study was pre-registered). Unexpectedly, higher trust failed to promote intuitive cooperation, though higher trust promoted cooperation. In addition, preferring to think intuitively or deliberatively failed to moderate the effect of trust on cooperation, although preferring to think intuitively increased cooperation. Moreover, deciding intuitively failed to promote cooperation, and equivalence testing confirmed that this null result was explained by the absence of an effect, rather than a lack of statistical power (equivalence bounds: d = -0.26 and 0.26). An intuitive cooperation effect emerged when non-compliant participants were excluded, but this effect could be due to selection biases. Taken together, most results failed to support the social heuristics hypothesis. We conclude by discussing implications, future directions, and limitations. The materials, data, and code are available on the Open Science Framework (https://osf.io/939jv/).
Hypoxia increases corneal cell expression of CFTR leading to increased Pseudomonas aeruginosa binding, internalization, and initiation of inflammation.

PURPOSE To investigate the effect of hypoxia-induced molecular responses of corneal epithelial cells on the surface of rabbit and human corneas and corneal cells in culture on interactions with Pseudomonas aeruginosa that may underlie increased susceptibility to keratitis. METHODS Organ cultures of rabbit and human corneal tissue, primary rabbit and human corneal cells, and transformed human corneal cells from a patient with cystic fibrosis and the same cell line corrected for expression of wild-type cystic fibrosis transmembrane conductance regulator (CFTR), the cellular receptor for P. aeruginosa, were exposed to hypoxic conditions for 24 to 72 hours. Changes in binding and internalization of P. aeruginosa were measured using cellular association and gentamicin-exclusion assays, and expression of CFTR and activation of NF-kappaB in response to hypoxia were determined by confocal laser microscopy and quantitative measurements of NF-kappaB activation. RESULTS Hypoxia induced in a time- and oxygen-concentration-dependent manner increased association and internalization of clinical isolates of P. aeruginosa in all cells tested. Hypoxia increased CFTR expression and NF-kappaB nuclear translocation in rabbit and human cells with wild-type CFTR. Corneal cells lacking CFTR had reduced NF-kappaB activation in response to hypoxia. Hypoxia did not affect the increase in corneal cell CFTR levels or NF-kappaB activation after P. aeruginosa infection. CONCLUSIONS Hypoxic conditions on the cornea exacerbate the binding and internalization of P. aeruginosa due to increased levels of CFTR expression and also induce basal NF-kappaB activation. Both of these responses probably exacerbate the effects of P. aeruginosa infection by allowing lower infectious doses of bacteria to induce disease and promote destructive inflammatory responses.
NMR-Based Tissular and Developmental Metabolomics of Tomato Fruit

Fruit is a complex organ containing seeds and several interconnected tissues with dedicated roles. However, most biochemical or molecular studies about fleshy fruit development concern the entire fruit, the fruit without seeds, or pericarp only. We studied tomato (Solanum lycopersicum) fruit at four stages of development (12, 20, 35, and 45 days post-anthesis). We separated the seeds and the other tissues, exocarp, mesocarp, columella with placenta and locular tissue, and analyzed them individually using proton NMR metabolomic profiling for the quantification of major polar metabolites, enzymatic analysis of starch, and LC-DAD analysis of isoprenoids. Pericarp tissue represented about half of the entire fruit mass only. The composition of each fruit tissue changed during fruit development. An ANOVA-PCA highlighted common, and specific metabolite trends between tissues e.g., higher contents of chlorogenate in locular tissue and of starch in columella. Euclidian distances based on compositional data showed proximities within and between tissues. Several metabolic regulations differed between tissues as revealed by the comparison of metabolite networks based on correlations between compounds. This work stressed the role of specific tissues less studied than pericarp but that impact fruit organoleptic quality including its shape and taste, and fruit processing quality.
Ehrlichia Isolate from a Minnesota Tick: Characterization and Genetic Transformation

Ehrlichioses are zoonotic diseases caused by intracellular bacteria that are transmitted by ixodid ticks. Here we report the culture isolation of bacteria which are closely related to, or the same as the Ehrlichia muris subsp. eauclairensis, a recently recognized human pathogen. EmCRT, obtained from a tick removed from deer at Camp Ripley, MN, is the second isolate of this subspecies described and is distinctive in that it was cultured directly from a field-collected tick. The isolate’s cellular tropism, pathogenic changes caused in rodent tissues, and tick transmission to and from rodents are detailed in this study. We also describe the genetic mutants created from the EmCRT isolate, which are valuable tools for the further study of this intracellular pathogen. ABSTRACT Ehrlichia muris subsp. eauclairensis is recognized as the etiological agent of human ehrlichiosis in Minnesota and Wisconsin. We describe the culture isolation of this organism from a field-collected tick and detail its relationship to other species of Ehrlichia. The isolate could be grown in a variety of cultured cell lines and was effectively transmitted between Ixodes scapularis ticks and rodents, with PCR and microscopy demonstrating a broad pattern of dissemination in arthropod and mammalian tissues. Conversely, Amblyomma americanum ticks were not susceptible to infection by the Ehrlichia. Histologic sections further revealed that the wild-type isolate was highly virulent for mice and hamsters, causing severe systemic disease that was frequently lethal. A Himar1 transposase system was used to create mCherry- and mKate-expressing EmCRT mutants, which retained the ability to infect rodents and ticks. IMPORTANCE Ehrlichioses are zoonotic diseases caused by intracellular bacteria that are transmitted by ixodid ticks. Here we report the culture isolation of bacteria which are closely related to, or the same as the Ehrlichia muris subsp. eauclairensis, a recently recognized human pathogen. EmCRT, obtained from a tick removed from deer at Camp Ripley, MN, is the second isolate of this subspecies described and is distinctive in that it was cultured directly from a field-collected tick. The isolate’s cellular tropism, pathogenic changes caused in rodent tissues, and tick transmission to and from rodents are detailed in this study. We also describe the genetic mutants created from the EmCRT isolate, which are valuable tools for the further study of this intracellular pathogen.
Effects of Different Biochars on Wheat Growth Parameters, Yield and Soil Fertility Status in a Silty Clay Loam Soil

The conversion of organic wastes into biochar via the pyrolysis technique could be used to produce soil amendments useful as a source of plant nutrients. In this study, we investigated the effects of fruit peels and milk tea waste-derived biochars on wheat growth, yield, root traits, soil enzyme activities and nutrient status. Eight amendment treatments were tested: no amendment (CK), chemical fertilizer (CF), banana peel biochar 1% (BB1 + CF), banana peel biochar 2% (BB2 + CF), orange peel biochar 1% (OB1 + CF), orange peel biochar 2% (OB2 + CF), milk tea waste biochar 1% (TB1 + CF) and milk tea waste biochar 2% (TB2 + CF). The results indicated that chlorophyll values, plant height, grain yield, dry weight of shoot and root were significantly (p < 0.05) increased for the TB2 + CF treatment as compared to other treatments. Similarly, higher contents of nutrients in grains, shoots and roots were observed for TB2 + CF: N (61.3, 23.3 and 7.6 g kg−1), P (9.2, 10.4 and 8.3 g kg−1) and K (9.1, 34.8 and 4.4 g kg−1). Compared to CK, the total root length (41.1%), surface area (56.5%), root volume (54.2%) and diameter (78.4%) were the greatest for TB2 + CF, followed by BB2 + CF, OB2 + CF, TB1 + CF, BB1 + CF, OB1 + CF and CF, respectively. However, BB + CF and OB + CF treatments increased β-glucosidase and dehydrogenase, but not urease activity, as compared to the TB + CF amendment, while all enzyme activity decreased with the increased biochar levels. We concluded that the conversion of fruit peels and milk tea waste into biochar products contribute the benefits of environmental and economic issues, and should be tested as soil amendments combined with chemical fertilizers for the improvement of wheat growth and grain yield as well as soil fertility status under field conditions.
On the presence and functional significance of sympathetic premotor neurons with collateralized spinal axons in the rat

Spinally‐projecting neurons of the rostral ventrolateral medulla (RVLM) determine sympathetic outflow to different territories of the body. Previous studies suggest the existence of RVLM neurons with distinct functional classes, such as neurons that target sympathetic nerves bound for functionally‐similar tissue types (e.g. muscle vasculature). The existence of RVLM neurons with more general actions had not been critically tested. Using viral tracing, we show that a significant minority of RVLM neurons send axon collaterals to disparate spinal segments (T2 and T10). Furthermore, optogenetic activation of sympathetic premotor neurons projecting to lumbar spinal segments also produced activation of sympathetic nerves from rostral spinal segments that innervate functionally diverse tissues (heart and forelimb muscle). These findings suggest the existence of individual RVLM neurons for which the axons branch to drive sympathetic preganglionic neurons of more than one functional class and may be able to produce global changes in sympathetic activity.
Editorial on Sramkova et al., "Apolipoprotein M: a novel adipokine decreasing with obesity and upregulated by calorie restriction".

In this issue of the Journal , Sramkova et al. present novel data from 5 separate clinical trials as well as an in vitro study that uses human multipotent adipose-derived stem cell (hMADS) adipocytes (1). The goal of this study was to determine the relation between apolipoprotein M (apo M) and its participation in metabolic syndrome and insulin sensitivity. In an appealing complementary ex vivo study, the authors examined the regulation of apo M expression and secretion by adipose tissue during caloric restriction and resulting weight loss. This study is significant for the following reasons: 1 ) most studies on apo M have been conducted in the liver and kidney (2–4), but we know very little of apo M function in human adipose tissue; 2 ) this is a helpful example of how interdisciplinary studies can lead to a major advancement in our understanding of the physiology and function of human adipose tissue apo M; and 3 ) as with other outstanding research, the completion of this study not only greatly enhances our knowledge of human adipose tissue apo M production and secretion, but also stimulates us to ask additional questions, such as how apo M affects insulin sensitivity and what the relation and interaction are between apo M production and secretion by the liver, kidney, and adipose tissue.Based on the use of a combination of microarray and proteomic analyses of the conditioned media from adipocytes and stromal vascular cells isolated from human adipose tissue, the authors identified apo M as a novel adipokine. The investigators then further validated apo M secretion from adipose tissue by detecting differential concentrations of apo M between and venous blood in the subcutaneous abdominal adipose tissue.
Optical Counterparts to Galaxies in the Cen A Group Discovered by HIPASS

Abstract We have completed a 21-cm survey of a 600 square degree region of the Centaurus A group of galaxies at a redshift of ∼500 km s−1 as part of a larger survey of the entire southern sky. This group of galaxies was recently the subject of a separate and thorough optical survey (Côté et al. 1997), and thus presented an ideal comparison for us to test the survey performance. We have identified 10 new group members to add to the 21 already known in our survey area. Six of the new members are previously uncatalogued galaxies, while four were catalogued but assumed not to be group members. Including the seven known members outside of our survey area, this brings the total known number of Cen A members to 38. All of the new HI detections have optical counterparts, most being intrinsically very faint (M B > –13·0), late-type low surface brightness dwarfs. Most of the new members have HI masses only a few times our survey limit of 107 M ☉ at an assumed distance for the group of 3·5 Mpc, and are extremely gas-rich, with a median M HI /L B > 1. Our limiting HI sensitivity was actually slightly worse than the HI follow-up observations of the Côté et al. optical survey, yet we have already increased the known number of group members by 50% using an HI survey technique. While we have increased the known number of members by ∼50%, these new members contribute <4% to its light.
Assessing language development in Arabic: The Arabic language: Evaluation of function (ALEF)

Abstract Arabic is characterized by extensive dialectal variation, diglossia, and substantial morphological complexity. Arabic lacks comprehensive diagnostic tools that would allow for a systematic evaluation of its development, critical for the early identification of language difficulties in the spoken and written domains. To address this gap, we have developed an assessment battery called Arabic Language: Evaluation of Function (ALEF), aimed at children aged 3 to 11 years. ALEF consists of 17 subtests indexing different language domains, modalities, and associated skills and representational systems. We administered the ALEF battery to native Gulf Arabic-speaking children (n = 467; ages 2.5 to 10.92; 55% boys; 20 children in each 6-month age band) in Saudi Arabia in two data collection waves. Analyses examining the psychometric properties of the instrument indicated that after the removal of misfitting items, the ALEF subtests had reliability coefficients in the range from 0.78 to 0.98, and resulting subtest scores displayed a consistent profile of positive intercorrelations and age effects. Taken together, the results indicate that the ALEF battery has good psychometric properties, and can be used for the purpose of evaluating early language development in Gulf Arabic speaking children, pending further refinement of the test structure, examination of gender-related differential item functioning, and norming.