Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Polyamines on Shoot Multiplication and Furanocoumarin Production in Ruta graveolens Cultures

The influence of the polyamines putrescine (Put), spermine (Spr) and spermidine (Spd) on growth and furanocoumarin production was investigated by exogenous addition, at different concentrations, to shoot cultures of Ruta graveolens at different phases of growth. Preliminary studies indicated that addition of Put (20 μM) and Spr (80 μM) had a promotive effect on shoot multiplication rate and number of multiple shoots formed. Spd was toxic, even at lower concentrations. The growth-phase of the culture at the time of exogenous addition of polyamines was found to be an important factor. Put was most effective when added at the lag phase, while Spr was most effective when added in the log phase. Time course studies of growth and furanocoumarin content were carried out for each polyamine and phase of addition. It was seen that maximum production of furanocoumarins (256.8 mg/10 g DW) occurred in the second week when Put was added in the lag phase and 260.5 mg/10 g DW in the fourth week when Spr was added in the log phase. Put addition resulted in a 3.10 fold increase in psoralen, 6.12 in xanthotoxin and 1.46 fold in bergapten production. Spr addition resulted in a 1.31 fold increase in psoralen, 4.11 fold in xanthotoxin and 1.49 fold in bergapten production. Results indicate that alteration of growth and furanocoumarin production kinetics is a combined outcome of choice of polyamine and the phase of culture at the time of exogenous addition. Polyamine addition enabled significant enhancement in production of pharmaceutically important bergapten and xanthotoxin in shoot cultures of Ruta graveolens, which could be explored for commercial production.
Comparing the pulmonary embolism severity index and the prognosis in pulmonary embolism scores as risk stratification tools.

BACKGROUND Multiple risk stratification scoring systems exist to forecast outcomes in patients with acute pulmonary embolism (PE). OBJECTIVE We evaluated the comparative validity of the PE severity index (PESI) and the prognosis in pulmonary embolism (PREP) scores to predict mortality in acute PE. DESIGN Retrospective observational cohort study. SETTING Washington Hospital Center, Washington, DC. PATIENTS Consecutive adults (aged >18 years) diagnosed with acute PE. INTERVENTION The PESI and PREP scores were calculated. MEASUREMENTS Raw PESI scores were segregated into risk class (I-V) and then dichotomized into low (I-II) versus high (III-V) risk groups; the raw PREP scores were divided into low (0-7) versus high (>7) risk groups. The primary endpoint was 30-day and 90-day mortality. We determined the negative predictive value and computed the area under the receiver operating characteristics (AUROC) curves to compare the ability of these scoring tools. RESULTS The cohort consisted of 302 subjects. Thirty-day mortality was 3.0%, and 4.0% died within 90 days. The PESI and the PREP performed similarly (PESI AUROC: 0.858 [95% confidence interval (CI), 0.773-0.943] vs 0.719 [95% CI, 0.563-0.875] for PREP). Segregating these scores into risk categories did not affect their discriminatory power (AUROC: 0.684 [95% CI, 0.559-0.810] for PESI and 0.790 [95% CI, 0.679-0.903] for PREP). The negative predictive value for death of being classified as low risk by the PESI or PREP was 100% and 99%, respectively. CONCLUSIONS The PREP score performed comparably to the PESI score for identifying PE patients at low risk for short-term and intermediate-term mortality.
An Automatic Student Verification System Utilising Off-Line Thai Name Components

This research proposed an automatic student identification and verification system utilising off-line Thai name components. The Thai name components consist of first and last names. Dense texture-based feature descriptors were able to yield encouraging results when applied to different handwritten text recognition scenarios. As a result, the authors employed such features in investigating their performance on Thai name component verification system. In this research, Dense-Local Binary Pattern, Dense-Local Directional Pattern, and Local Binary Pattern combined with Local Directional Pattern were employed. A base-line shape/feature i.e. Hidden Markov Model (HMM) was also utilised in this study. As there is no dataset on Thai name verification in the literature, a dataset is proposed for a Thai name verification system. The name component samples were collected from high school students. It consists of 8,400 name components (first and last names) from 100 students. Each student provided 60 genuine name components, and each of the name components was forged by 12 other students. An encouraging result was found employing the above-mentioned features on the proposed dataset.
Exploring school effects across southern and eastern African school systems and in Tanzania

The Southern and Eastern Africa Consortium for Monitoring Educational Quality (SACMEQ) II data are analysed, using multilevel modelling techniques, to explore the key issues underlying the development of school effectiveness models. Differences between schools in Grade 6 pupils’ reading and mathematics achievements are examined and the percentage of variance in pupil outcomes attributable to school and country levels is estimated before and after adjusting for various factors. A wide range of explanatory factors has been investigated, such as pupil gender, age, socio‐economic status, homework, absence, grade repetition, school location, resources, leadership and community involvement. Only a minority of these factors was found to be statistically significantly related to pupil achievements. Tanzanian educational stakeholder views on the implications of the SACMEQ II analysis findings are also reported. In conclusion the findings suggest that a contextualised approach incorporating stakeholders’ views is needed to build understanding of school effectiveness in low‐income countries.
Up-regulation of microRNAs in brain of human alcoholics.

BACKGROUND MicroRNAs (miRNAs) are small, noncoding oligonucleotides with an important role in posttranscriptional regulation of gene expression at the level of translation and mRNA degradation. Recent studies have revealed that miRNAs play important roles in a variety of biological processes, such as cell proliferation, neuronal differentiation, developmental timing, synapse function, and neurogenesis. A single miRNA can target hundreds of mRNA transcripts for either translation repression or degradation, but the function of many human miRNAs is not known. METHODS miRNA array analysis was performed on the prefrontal cortex of 27 individual human cases (14 alcoholics and 13 matched controls). Target genes for differentially expressed miRNAs were predicted using multiple target prediction algorithms and a consensus approach, and predicted targets were matched against differentially expressed mRNAs from the same samples. Over- and under-representation analysis was performed using hypergeometric probability and z-score tests. RESULTS Approximately 35 miRNAs were significantly up-regulated in the alcoholic group compared with controls. Target prediction showed a large degree of overlap with our published cDNA microarray data. Functional classification of the predicted target genes of the regulated miRNAs includes apoptosis, cell cycle, cell adhesion, nervous system development, and cell-cell signaling. CONCLUSIONS These data suggest that the reduced expression of genes in human alcoholic cases may be because of the up-regulated miRNAs. Cellular processes fundamental to neuronal plasticity appear to represent major targets of the suggested miRNA regulation.
Achievement Emotions in Germany and China

The aim of this study was to establish the construct comparability and cross-cultural utility of the German and Chinese versions of the Academic Emotions Questionnaire—Mathematics (AEQ-M). Based on data from 312 German and 579 Chinese eighth-grade students, mean and covariance structures analysis revealed that the AEQ-M shows a high degree of measurement invariance across cultures. In addition, the emotions assessed by the AEQ-M showed similar patterns of relationships with self-reports of achievement, parental achievement expectations, and attributions of success and failure across the German and Chinese samples. Confirming earlier findings, Chinese students were found to experience higher levels of anxiety in mathematics. They were also found to experience more enjoyment, pride, and shame, and less anger, than German students. This research supports the use of the AEQ-M in cross-cultural research and provides data about a broader range of achievement emotions than has been investigated previously.
Relational characteristics of language: Elaboration and differentiation in marital conversations

Our research considered how the language used in marital conversations reflects the nature and definition of close relationships. Two linguistic themes with broad relational implications were identified from previous work: (a) linguistic elaboration, and (b) personal reference. These features were assumed to reflect, respectively, the efficiency of communication and the degree of differentiation versus integration of identities in marriage. In order to test the presumed relational implications of language, we compared indicators of linguistic elaboration and personal reference with relationship characteristics, including marital type, satisfaction, and age. One hundred‐twenty conversations from three prior studies were coded for a number of linguistic and pragmatic features. The results did not support expected associations between elaboration and other relationship characteristics. However, the research mostly supported the presumed relational implications of personal reference. Traditional, satisfied, a.
The contribution of age and reading instruction to oral narrative and pre-reading skills

Research suggests children beginning school around age five years show similar long-term reading achievement as children who start later, at seven years. To shed light on this phenomenon, this article presents cross-sectional data examining the oral narrative, phonemic awareness and non-word decoding skills of three groups of children at the beginning of state schooling (age 5), the beginning of Waldorf schooling (age 7) and children who attended state schooling, but were of a similar age to the Waldorf sample (age 7) (N = 103). Key covariates of receptive vocabulary, home literacy environment, sex, ethnicity and maternal education were included. Analyses suggested language development – including story memory and narrative quality and phoneme awareness – improved with age but not length of formal schooling. Conversely, non-word decoding skills improved with formal schooling, but not age. These findings add to the literature supporting separate skill clusters of language and decoding skills, with potentially different contributors to their development.
Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and Antimicrobial Resistance

The risks of contracting staphylococci food poisoning by the consumption of improperly manufactured salami and the possibility of this food being reservoirs for antibiotic resistance were evaluated. Nineteen coagulase-negative staphylococci (CNS) strains were found in commercial and artisanal salami. The species in commercial salami were S. saprophyticus, S. sciuri, S. xylosus, and S. carnosus. Artisanal salami showed S. succinus, S. epidermidis, and S. hominis but no S. carnosus. Phylogenetic analyses grouped the strains into three major staphylococcal species groups, comprised of 4 refined clusters with similarities superior to 90%. Fifteen strains harbored multiple enterotoxin genes, with high incidence of seb/sec and sea, 57% and 50%, respectively, intermediate incidence of sed/seh/selm and sei/seln/tst-H, 33% and 27%, correspondingly, and low incidence of see/selj/selo and seg, of respectively 13% and 1%. Real time RT-PCR and enzyme-linked-immunosorbent assays confirmed the enterotoxigenicity of the strains, which expressed and produced enterotoxins in vitro. The CNS strains showed multiresistance to several antimicrobials of therapeutic importance in both human and veterinarian medicine, such as β-lactams, vancomycin, and linezolid. The effective control of undue staphylococci in fermented meat products should be adopted to prevent or limit the risk of food poisoning and the spread of antimicrobial-resistant strains.
Access to Higher Education for the Disabled Student: A building survey at the University of Liverpool

The problems associated with disabled students gaining access to higher education are complex and can be complicated further by difficulties encountered in the built environment of universities themselves. A survey of the built environment at the University of Liverpool was carried out using a group of occupational therapy undergraduate students. Students were able to learn about access audits and understand more fully the problems associated with access as part of their own studies. Results were recorded using a computer database, which enabled dissemination of information via the university's managed PC network and Home pages on the internet. Discussion and debate centre on the dilemma between a freely accessible environment for all students and the constraints imposed by the present day built environment and economic climate. The need for effective strategies in collaboration with disabled students is also considered.
Surface Charge Accumulation on Silicon Rubber Material under DC Voltage in Atmosphere

The charge accumulation on the surface of dielectric materials is a long-term academic research focus. As part of an effort to understand the origins of this behavior, surface charge distribution on silicon rubber under DC high voltage before and after flashover in atmosphere was measured by an electrostatic voltmeter with a vibrating Kelvin probe. Three types of electrode systems were employed in the study, i.e. circle-circle electrodes, ellipse-circle electrodes and tip-circle electrodes. The results showed that surface charging processes are close related with uniformity of electric field and voltage polarities. Once flashover happens, there exists a channel linking HV and grounded electrodes, which presents a low potential.
‘Control – What Control?’ Culture and Ambiguity Within a Knowledge Intensive Firm*

This paper explores the distinctive culture that existed within a knowledge-intensive firm (KIF) and also attempts to explain the emergence and effects of this culture. The findings are based on a detailed case study that was conducted over two years within a consultancy firm that created and applied scientific knowledge and expertise to the invention of solutions for clients. The firm employed highly educated scientists, considered 'leading' in their respective disciplines and project work was inherently fluid, complex, and uncertain. These kinds of 'knowledge workers', and this kind of work, are expected to demand high levels of autonomy. This creates complex managerial dilemmas around how to balance autonomy with control and uncertainty and flexibility with efficiency. The analysis shows how a strong culture based on an acceptance of ambiguity (e.g. in roles, power relations, organizational routines and practices) promoted the development of a loyal, committed, effective workforce and sustained a fluid and flexible form of project working over time. Critically, ambiguity allowed individuals to sustain multiple identities as both 'expert' and 'consultant'. This, coupled with a corporate identity premised on 'elitism', helped to maximize commitment to the work and minimize tensions between control and autonomy. Thus the culture that embraced ambiguity (a consensus that there would be no consensus) engendered a form of normative control whereby consultants operated freely and at the same time willingly participated in the regulation of their own autonomy. Copyright Blackwell Publishers Ltd 2003.
A Comparison of On-Campus and Distance Teacher Education Programs in Severe Disabilities

Distance teacher education programs employing videoconferencing technology and other online teaching tools are increasingly being used to prepare teachers of students with disabilities in rural and remote communities. This evaluation study compared the effectiveness of a distance and an on-campus teacher education program for teachers of students with severe disabilities. Teacher candidates in the distance cohort received the same program of study as candidates in the on-campus program except that the coursework was delivered through videoconferencing technology instead of traditional, face-to-face instruction. Instructional activities for candidates in both cohorts were supported through online instructional tools (i.e., Web CT) and on-site field supervision from university clinical faculty members and cooperating professionals from the local school districts. A variety of measures were used to assess candidates’ acquisition of content and their ability to apply the knowledge and skills acquired through the program of study. The results indicate that there were no significant differences between students in the distance and on-campus teacher education cohorts on the measures of learning. The implications of the findings for further research are discussed.
Effects of Deoxycholylglycine, a Conjugated Secondary Bile Acid, on Myogenic Tone and Agonist-Induced Contraction in Rat Resistance Arteries

Background Bile acids (BAs) regulate cardiovascular function via diverse mechanisms. Although in both health and disease serum glycine-conjugated BAs are more abundant than taurine-conjugated BAs, their effects on myogenic tone (MT), a key determinant of systemic vascular resistance (SVR), have not been examined. Methodology/Principal Findings Fourth-order mesenteric arteries (170–250 µm) isolated from Sprague-Dawley rats were pressurized at 70 mmHg and allowed to develop spontaneous constriction, i.e., MT. Deoxycholylglycine (DCG; 0.1–100 µM), a glycine-conjugated major secondary BA, induced reversible, concentration-dependent reduction of MT that was similar in endothelium-intact and -denuded arteries. DCG reduced the myogenic response to stepwise increase in pressure (20 to 100 mmHg). Neither atropine nor the combination of L-NAME (a NOS inhibitor) plus indomethacin altered DCG-mediated reduction of MT. K+ channel blockade with glibenclamide (KATP), 4-aminopyradine (KV), BaCl2 (KIR) or tetraethylammonium (TEA, KCa) were also ineffective. In Fluo-2-loaded arteries, DCG markedly reduced vascular smooth muscle cell (VSM) Ca2+ fluorescence (∼50%). In arteries incubated with DCG, physiological salt solution (PSS) with high Ca2+ (4 mM) restored myogenic response. DCG reduced vascular tone and VSM cytoplasmic Ca2+ responses (∼50%) of phenylephrine (PE)- and Ang II-treated arteries, but did not affect KCl-induced vasoconstriction. Conclusion In rat mesenteric resistance arteries DCG reduces pressure- and agonist-induced vasoconstriction and VSM cytoplasmic Ca2+ responses, independent of muscarinic receptor, NO or K+ channel activation. We conclude that BAs alter vasomotor responses, an effect favoring reduced SVR. These findings are likely pertinent to vascular dysfunction in cirrhosis and other conditions associated with elevated serum BAs.
Relationship between renal dysfunction, nephrotoxicity and death among HIV adults on tenofovir

Objective:In April 2010, the South African government added tenofovir disoproxil fumarate to its first-line antiretroviral therapy (ART) for HIV patients. We analyzed the relationship between renal dysfunction at tenofovir initiation, nephrotoxicity and mortality. Design:Cohort analysis of HIV-infected adults who received tenofovir and had a creatinine clearance done at initiation at the Themba Lethu Clinic, Johannesburg, South Africa, between April 2004 and September 2009. Methods:We estimated the relationship between renal dysfunction, nephrotoxicity [any decline in kidney function from baseline (acute or chronic) that is secondary to a toxin (including drugs)] and mortality for patients initiated onto tenofovir-containing regimens using marginal structural models and inverse probability of treatment weights to correct estimates for lost to follow-up and confounding. Results:Of 890 patients initiated onto tenofovir, 573 (64.4%) had normal renal function (≥90 ml/min), 271 (30.4%) had mild renal dysfunction (60–89 ml/min) and 46 (5.2%) had moderate renal dysfunction (30–59 ml/min). A total of 2.4% experienced nephrotoxicity, 7.8% died and 9.7% were lost during 48 months of follow-up. Patients with mild [hazard ratio 4.8; 95% confidence interval (CI) 1.5–15.2] or moderate (hazard ratio 15.0; 95% CI 3.4–66.5) renal dysfunction were at greatest risk of nephrotoxicity, whereas those with mild (hazard ratio 1.2; 95% CI 0.7–2.3) or moderate (hazard ratio 3.2; 95% CI 1.3–7.8) renal dysfunction vs. normal renal function were at highest risk of death by 48 months. Conclusion:Much of the incident renal dysfunction in tenofovir patients is likely related to preexisting renal disorder, which may be exacerbated by tenofovir. With expanded use of tenofovir, screening for renal dysfunction prior to initiation and dose adjustment is necessary to help improve ART outcomes.
Emotion identification in girls at high risk for depression.

BACKGROUND Children of depressed mothers are themselves at elevated risk for developing a depressive disorder. We have little understanding, however, of the specific factors that contribute to this increased risk. This study investigated whether never-disordered daughters whose mothers have experienced recurrent episodes of depression during their daughters' lifetime differ from never-disordered daughters of never-disordered mothers in their processing of facial expressions of emotion. METHOD Following a negative mood induction, daughters completed an emotion identification task in which they watched faces slowly change from a neutral to a full-intensity happy, sad, or angry expression. We assessed both the intensity that was required to accurately identify the emotion being expressed and errors in emotion identification. RESULTS Daughters of depressed mothers required greater intensity than did daughters of control mothers to accurately identify sad facial expressions; they also made significantly more errors identifying angry expressions. CONCLUSION Cognitive biases may increase vulnerability for the onset of disorders and should be considered in early intervention and prevention efforts.