Miracle-dz/UniFAR-FTUs huggingface dataset:
Distribution of 5-HT3, 5-HT4, and 5-HT7 Receptors Along the Human Colon

Background/Aims Several disorders of the gastrointestinal tract are associated with abnormal serotonin (5-HT) signaling or metabolism where the 5-HT3 and 5-HT4 receptors are clinically relevant. The aim was to examine the distribution of 5-HT3, 5-HT4, and 5-HT7 receptors in the normal human colon and how this is associated with receptor interacting chaperone 3, G protein coupled receptor kinases, and protein LIN-7 homologs to extend previous observations limited to the sigmoid colon or the upper intestine. Methods Samples from ascending, transverse, descending, and sigmoid human colon were dissected into 3 separate layers (mucosa, longitudinal, and circular muscles) and ileum samples were dissected into mucosa and muscle layers (n = 20). Complementary DNA was synthesized by reverse transcription from extracted RNA and expression was determined by quantitative or end point polymerase chain reaction. Results The 5-HT3 receptor subunits were found in all tissues throughout the colon and ileum. The A subunit was detected in all samples and the C subunit was expressed at similar levels while the B subunit was expressed at lower levels and less frequently. The 5-HT3 receptor E subunit was mainly found in the mucosa layers. All splice variants of the 5-HT4 and 5-HT7 receptors were expressed throughout the colon although the 5-HT4 receptor d, g, and i variants were expressed less often. Conclusions The major differences in 5-HT receptor distribution within the human colon are in relation to the mucosa and muscular tissue layers where the 5-HT3 receptor E subunit is predominantly found in the mucosal layer which may be of therapeutic relevance.
A Multicenter Experience With the Placement of Self-Expanding Metallic Tracheobronchial Y Stents

Background:Deployment of a bifurcation (Y) stent is often required in patients with airway obstruction or fistulization near the tracheal carina. Herein, we describe our experience with placement of self-expanding metallic Y stents. Methods:This was a retrospective analysis of data of consecutive subjects who underwent placement of self-expanding metallic Y stent over 2 years at 6 different centers. We describe the technique, complications, and outcomes of metallic Y stent placement at the tracheal carina. Results:Thirty-eight subjects (25 men) with a mean age of 54.8 years underwent Y stent placement. The most common underlying disease was carcinoma of the esophagus (65.8%). The most common indication for stent insertion was central airway obstruction in 30 (78.9%) subjects followed by airway-esophageal fistula. Respiratory failure was present in 17 (44.7%) subjects at admission. The Y stent was deployed using either the rigid (n=32) or the flexible (n=6) bronchoscope, and was successfully placed in 37 of the 38 (97.4%) subjects. There was rapid improvement in symptoms and subsequent resolution of respiratory failure after stent placement. There was no periprocedural mortality and few stent-related complications. On follow-up at 12 weeks, 18 patients had died due to progression of the underlying disease. Conclusion:The insertion of a metallic Y stent results in immediate palliation of malignant airway obstruction or airway fistulization near the tracheal carina with rapid improvement of symptoms.
Home 3D body scans from noisy image and range data

The 3D shape of the human body is useful for applications in fitness, games and apparel. Accurate body scanners, however, are expensive, limiting the availability of 3D body models. We present a method for human shape reconstruction from noisy monocular image and range data using a single inexpensive commodity sensor. The approach combines low-resolution image silhouettes with coarse range data to estimate a parametric model of the body. Accurate 3D shape estimates are obtained by combining multiple monocular views of a person moving in front of the sensor. To cope with varying body pose, we use a SCAPE body model which factors 3D body shape and pose variations. This enables the estimation of a single consistent shape while allowing pose to vary. Additionally, we describe a novel method to minimize the distance between the projected 3D body contour and the image silhouette that uses analytic derivatives of the objective function. We propose a simple method to estimate standard body measurements from the recovered SCAPE model and show that the accuracy of our method is competitive with commercial body scanning systems costing orders of magnitude more.
Perceptuo-motor biases in the perceptual organization of the height feature in French vowels

This paper reports on the organization of the perceived vowel space in French. In a previous paper [28], we investigated the implementation of vocal height contrasts along the F1 dimension in French speakers. In this paper, we present results from perceptual identification tests performed by twelve participants who took part in the production experiment reported in the earlier paper. For each subject, stimuli presented in the identification test were synthesized in two different vowel spaces, corresponding to two different vocal tract lengths. The results showed that first, the perceived French vowels belonging to similar height degrees were aligned on stable F1 values, independent of place of articulation and roundedness, as was the case for produced vowels. Second, the produced F1 distances between height degrees correlated with the perceived F1 distances. This suggests that there is a link between perceptual and motor phonemic prototypes in the human brain. The results are discussed using the framework of the Perception for Action Control (PACT) theory, in which speech units are considered to be gestures shaped by perceptual processes.
Rapid response teams: Current perspectives

Abstract: Rapid response teams (RRTs) have been in existence for nearly 25 years.1 The team's purpose is to assess and manage patients who experience acute clinical deterioration. The critical care nurse performs a vital role in the function of the team. This article reviews the composition, responsibilities, and common challenges of RRTs.
The ban on smokeless tobacco products is systematically violated in Chennai, India.

BACKGROUND India is the world's third largest consumer of tobacco. There are twice as many users of smokeless tobacco products (STPs) as cigarette smokers. The Government of Tamil Nadu has banned the sale of gutkha and pan masala in 2013. Our aim was to identify the varieties of illegal STP available in Chennai, India. METHODS In systematically chosen zones and regions of Chennai city, we randomly identified three kinds of kiosks (n = 18) and asked for "gutkha" and "pan masala," one of each product available. Details of each product were reviewed based on the information printed on the sachets. RESULTS Totally 65 STPs were collected; 26 distinct products and 23 brands. All products were claimed to be "tobacco" by the shop keepers. Sixty-five percent of the products informed to contain tobacco and 15.4% to contain pan masala. Five sachets did not inform about the content; 30.8% did not have a pictorial warning; a text warning was printed on 80.8%, but only two products had the messages in Tamil; 70% had promotional messages printed, and 57% had their registration numbers printed. CONCLUSION The ban on STP is being systematically violated in Chennai. STP are cheap and easily available and due to promotional laudatory messages and lacking information about the content and warning of health damage, the consumers are left with the perception that they buy more or less harmless product. The Indian Government must introduce policies to control production, import, and sale of illicit STP but we also call for a coordinated international solution.
Uploading speaking assignment to YouTube channel as an effort in increasing student’s pronunciation skill

YouTube is an internet channel in which people can freely find, watch, and upload any videos without any particular regulation. Learning motivation can be achieved in many ways. One of those is by uploading speaking assignment to YouTube channel,and a lot of people can watch it. This can be a strong motivation of the students to practice their pronunciation as well as possible. The objective of this study was to investigate student’s motivation in learning pronunciation by uploading their speaking assignment to YouTube. The study employed qualitative study with descriptive analytical approach. Qualitative study was chosen to dig deeper information related to the focus of the study. The instrument of the study was an open questioner with 5 questions related to student’s motivation in learning pronunciation. The study applied random sampling technique with 10 informants who had submitted their speaking assignment to YouTube. The study showed that uploading speaking videos in YouTube could increase student’s motivation. 80 percent of the respondents revealed that before they made their videos, they prepared and rehearsed to have correct pronunciation. In conclusion, uploading video in you tube channel had increased student’s motivation in mastering English pronunciation. DOI: 10.26905/enjourme.v2i2. 2741
Outcome of Laproscopic Treatment of Chronic Pelvic Pain with Endometriosis Cases Followed by Post Operative Progestogens

Objective: This is a Prospective randomized analytical study carried out in department of obstetrics and Gynecology, Hi-Tech Medical College & Hospital, Bhubaneswar, in a tertiary care center to compare the efficacy and safety of dienogest (DNG) with depot leuprolide acetate (LA) in patients with chronic pelvic pain following laparoscopic surgery for endometriosis. Materials & Method: 242 patients with chronic pelvic pain following laparoscopic surgery for endometriosis were chosen. Prospective randomized trial was done in laproscopic treated patient of endometriosis with chronic pelvic pain with Dienogest (2 mg/day, orally) or depot LA (3.75 mg/4 weeks, intramuscularly) for 12 weeks. The study was conducted over 3year period i.e. from 01.04. 2016 to 31.03. 2019 in Hi-Tech Medical College & Hospital, Bhubaneswar. A visual analogue scale was used to test the intensity of pain before and after the end of treatment. Results: There was highly significant reduction in pelvic pain, back pain and dyspareunia in both groups with mean of difference in dienogest group (28.7±5.3,19.0±4.3 and 20.0 ± 3.08 mm, respectively) and in LA group (26.2 ± 3.01, 19.5 ± 3.01 and 17.9 ± 2.9 mm, respectively). The most frequent drugrelated adverse effects in dienogest group were vaginal bleeding and weight gain (64.5 and 10.8%, respectively) which were significantly higher than LA group (21.5 and 3.3%, respectively). While the most frequent drug-related adverse effects in LA group were hot flushes and vaginal dryness (46.3 and 15.7%, respectively) which were significantly higher than dienogest group (15.7 and 3.3%, respectively). Conclusion: Daily dienogest is as effective as depot LA for relieving endometriosis-associated pelvic pain, low back pain and dyspareunia. In addition, dienogest has acceptable safety, tolerability and lower incidence of hot flushes. Thus, it may offer an effective and well-tolerated treatment in endometriosis
Probing the Spt16-Pob3 Interface Through BPA Cross-Linking in Saccharomyces cerevisiae as a Proof of Concept

SDS-PAGE and Western Blot to confirm proteinprotein interactions in vivo Abstract Examining protein-protein interactions in vivo can often be challenging due to nonspecific cross reactivity. The purpose of this study is to determine the effectiveness of the BPA cross-linking technique in Saccharomyces cerevisiae by confirming protein-protein interactions between FACT subunits: Spt16 and Pob3. We concluded that the BPA cross-linking technique was effective in confirming the protein-protein interactions between FACT subunits within the dimerization domain. Introduction Eukaryotic DNA is packaged into linear chromosomes with the aid of nucleosomes. Nucleosomes are structures consisting of 145-147 bp DNA wrapped around a histone octamer (McGinty and Tan, 2015). For transcription to proceed, the nucleosomal structure needs to be loosened in order for transcriptional machinery to access the DNA (McGinty and Tan, 2015). FACT (Facilitates Chromatin Transcription) is a nuclear protein in S. cerevisiae that is composed of two subunits: Spt16 and Pob3, which aids in nucleosomal reorganization to facilitate transcriptional processes (Winkler and Luger, 2011).
Translocated LPS Might Cause Endotoxin Tolerance in Circulating Monocytes of Cystic Fibrosis Patients

Cystic Fibrosis (CF) is an inherited pleiotropic disease that results from abnormalities in the gene codes of a chloride channel. The lungs of CF patients are chronically infected by several pathogens but bacteraemia have rarely been reported in this pathology. Besides that, circulating monocytes in CF patients exhibit a patent Endotoxin Tolerance (ET) state since they show a significant reduction of the inflammatory response to bacterial stimulus. Despite a previous description of this phenomenon, the direct cause of ET in CF patients remains unknown. In this study we have researched the possible role of microbial/endotoxin translocation from a localized infection to the bloodstream as a potential cause of ET induction in CF patients. Plasma analysis of fourteen CF patients revealed high levels of LPS compared to healthy volunteers and patients who suffer from Chronic Obstructive Pulmonary Disease. Experiments in vitro showed that endotoxin concentrations found in plasma of CF patients were enough to induce an ET phenotype in monocytes from healthy controls. In agreement with clinical data, we failed to detect bacterial DNA in CF plasma. Our results suggest that soluble endotoxin present in bloodstream of CF patients causes endotoxin tolerance in their circulating monocytes.
Non-eosinophilic asthma: importance and possible mechanisms

There is increasing evidence that inflammatory mechanisms other than eosinophilic inflammation may be involved in producing the final common pathway of enhanced bronchial reactivity and reversible airflow obstruction that characterises asthma. A review of the literature has shown that, at most, only 50% of asthma cases are attributable to eosinophilic airway inflammation. It is hypothesised that a major proportion of asthma is based on neutrophilic airway inflammation, possibly triggered by environmental exposure to bacterial endotoxin, particulate air pollution, and ozone, as well as viral infections. If there are indeed two (or more) subtypes of asthma, and if non-eosinophilic (neutrophil mediated) asthma is relatively common, this would have major consequences for the treatment and prevention of asthma since most treatment and prevention strategies are now almost entirely focused on allergic/eosinophilic asthma and allergen avoidance measures, respectively. It is therefore important to study the aetiology of asthma further, including the underlying inflammatory profiles.
Mapping and determinism of soil microbial community distribution across an agricultural landscape

Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communities at 278 sites across a monitored agricultural landscape of 13 km². Molecular microbial biomass was estimated by soil DNA recovery and bacterial diversity by 16S rRNA gene pyrosequencing. Geostatistics provided the first maps of microbial community at this scale and revealed a heterogeneous but spatially structured distribution of microbial biomass and diversity with patches of several hundreds of meters. Variance partitioning revealed that both microbial abundance and bacterial diversity distribution were highly dependent of soil properties and land use (total variance explained ranged between 55% and 78%). Microbial biomass and bacterial richness distributions were mainly explained by soil pH and texture whereas bacterial evenness distribution was mainly related to land management. Bacterial diversity (richness, evenness, and Shannon index) was positively influenced by cropping intensity and especially by soil tillage, resulting in spots of low microbial diversity in soils under forest management. Spatial descriptors also explained a small but significant portion of the microbial distribution suggesting that landscape configuration also shapes microbial biomass and bacterial diversity.
Phydbac (phylogenomic display of bacterial genes): an interactive resource for the annotation of bacterial genomes

Phydbac is a web interactive resource based on phylogenomic profiling, designed to help microbiologists to annotate bacterial proteins. Phylogenomic annotation is based on the assumption that functionally linked protein-coding genes must evolve in a coordinated manner. The detection of subsets of co-evolving genes within a given genome involves the computation of protein sequence conservation profiles across a spectrum of microbial species, followed by the identification of significant pairwise correlations between them. Many ongoing studies are devoted to the problem of computing the most biologically significant phylogenomic profiles and how best identifying clusters of 'functionally interacting' genes. Here we introduce a web tool, Phydbac, allowing the dynamic construction of phylogenomic profiles of protein sequences of interest and their interactive display. In addition, Phydbac can identify Escherichia coli proteins exhibiting the evolution pattern most similar to arbitrary query protein sequences, hence providing functional hints for open reading frames (ORFs) of hypothetical or unknown function. The phylogenomic profiles of all E.coli K-12 protein-coding genes are pre-computed, allowing queries about E.coli genes to be answered instantaneously. The profiles and phylogenomic neighborhoods are computed using an original method shown to perform better than previous ones. An extension of Phydbac, including precomputed profiles for all available bacterial genomes (including major pathogens) will soon be available. Phydbac can be accessed at: http://igs-server.cnrs-mrs.fr/phydbac/.
MEMBRANE IONIC CURRENT PHOTOMODIFICATION BY ROSE BENGAL AND MENADIONE: ROLE OF SINGLET OXYGEN

Abstract: Photosensitized modification of ionic leak current and potassium current was studied in frog cardiac atrial cells using whole cell patch clamp techniques. Rose bengal (RB) and menadione (MQ) were used as photosensitizers. Separate photophysical studies of the photosensitizers in deuterium oxide solution demonstrated that MQ did not produce singlet oxygen as evidenced by the lack of luminescence at 1270 nm, whereas RB was an efficient singlet oxygen generator. Both photosensitizers sensitized block of potassium current in atrial cells, and both sensitized an increase of ionic leak current. However, when photosensitizer concentrations and illumination intensities were adjusted to match the rate of block of potassium current by the two photosensitizers, there were dramatic differences in leak current increase, both quantitatively and qualitatively. Menadione sensitized a much slower increase in leak current than did RB. Further, the leak current sensitized by MQ had a more positive reversal potential than that sensitized by RB, suggesting a less potassium‐selective leak current pathway. The results suggest that, while the effects of singlet oxygen and non‐singlet oxygen modification of cell membranes may be similar, there may also be significant differences in the resulting membrane permeabilities. The results also demonstrate that MQ and RB may be useful agents to study the role of singlet oxygen versus non‐singlet oxygen modification of biological systems.
A Comparison of Liver Function After Hepatectomy in Cirrhotic Patients Between Sevoflurane and Isoflurane in Anesthesia with Nitrous Oxide and Epidural Block

In this study, we compared postoperative liver function in patients with liver cirrhosis between isoflurane and sevoflurane anesthesia with nitrous oxide (N2O) and epidural block. Forty cirrhotic patients with Child-Pugh Grade A, aged 40 to 70 yr, scheduled for liver segmentectomy, had anesthesia induced with midazolam 0.1 mg/kg and fentanyl 4 &mgr;g/kg. For maintenance, intermittent epidural administration of 1.5% lidocaine 4 to 6 mL and sevoflurane (sevoflurane group) or isoflurane (isoflurane group) with N2O 3 L/min in oxygen 3 L/min was used. Aspartate aminotransferase, alanine aminotransferase, total bilirubin, alkaline phosphatase, choline esterase, albumin, prothrombin time, and platelet count were measured before and 1, 3, and 7 days after surgery. Aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase increased significantly, with the peaks at 3 days after surgery in both groups. The increases in these variables were significantly larger in the isoflurane group than those in the sevoflurane group. No patient developed hepatic failure. All increases in liver enzymes were small and of questionable clinical relevance. Whether sevoflurane might be a better anesthetic when combined with N2O and epidural block for cirrhotic patients than isoflurane with respect to liver damage remains to be determined.
An Effective Resource Management Scheme for UWB Networks with Simultaneous Transmissions

This paper aims at an effective resource management scheme for ultra-wideband (UWB) networks where the inherent spread spectrum supports simultaneous transmissions. In specific, we present a transmission frame structure tailoring to the UWB characteristics, and develop a novel control message exchange procedure. Furthermore, we propose effective admission control and resource allocation algorithms to achieve high efficiency. The resource management scheme can solve the near-sender-blocking problem and alleviate the negative effect of long acquisition time in UWB transmissions. Extensive simulations demonstrate the superior performance of our proposed scheme.
Dimensions of Information Systems Success

The value added by an organization’s IT assets is a critical concern to both research and practice. Not surprisingly, a large number of IS effectiveness measures can be found in the IS literature. What is not clear in the literature is what measures are appropriate in a particular context. In this paper we propose a two-dimensional matrix for classifying IS Effectiveness measures. The first dimension is the type of system studied. The second dimension is the stakeholder in whose interests the system is being evaluated. The matrix was tested by using it to classify IS effectiveness measures from 186 empirical papers in three major IS journals for the last nine years. The results indicate that the classifications are meaningful. Hence, the IS Effectiveness Matrix provides a useful guide for conceptualizing effectiveness measurement in IS research, and for choosing appropriate measures, both for research and practice.