Miracle-dz/UniFAR-FTUs huggingface dataset:
Neuroimaging and neuroethics: clinical and policy considerations

Purpose of reviewNeuroimaging is providing a window on severe brain injury and mechanisms of recovery in patients with consciousness disorders. This brief essay will consider the ethical implications of these developments for clinical diagnosis, emerging therapeutics and health policy. Recent findingsOver the past year, functional imaging has led to two dramatic findings with the potential to alter how clinicians categorize severe brain injury and understand mechanisms of recovery. One paper reported a neuroimaging response in a patient who was behaviorally in the vegetative state. Another described structural changes in a patient with late emergence from the minimally conscious state. SummaryResearch into functional imaging and disorders of consciousness have the potential to elucidate mechanisms of brain recovery and refine their diagnostic nosology. These distinctions are becoming increasingly important given the potential of emerging therapeutic approaches to the minimally conscious state. Despite this promise the refinement of descriptive categories such as the vegetative and minimally conscious states is not without some degree of peril. Investigators and clinicians need to appreciate the uncertainty that will be engendered as diagnostic categories are redrawn and resist the premature dissemination of neuroimaging technology before its ethical implications for clinical practice are well understood.
Quality of life in patients with cutaneous leishmaniasis.

BACKGROUND Leishmaniasis is a zoonotic infection caused by a protozoa belonging to the genus Leishmania. Its clinical manifestations range from a self-healing cutaneous leishmaniasis (CL) to lethal visceral leishmaniasis. We aim to examine the quality of life of patients with CL in Kerman, Iran. METHODS In this cross-sectional study we evaluated 124 patients with CL. The Dermatology Life Quality Index (DLQI) questionnaire was used for measuring quality of life. Data on demographics and characteristics of the lesions also were collected. Mann-Whitney U-test and Kruskal-Wallis were used for data analyses. RESULTS The mean DLQI score was 5.87 ± 5.96. We observed the highest effect in the symptoms and feelings domains; the lowest effect was seen in the treatment domain of the DLQI. There was no significant difference in DLQI scores between men and women. Patients with ulcerated lesions had lower quality of life (P < 0.05). CONCLUSION CL significantly affects the quality of life of patients. Further studies are suggested to examine the effect of its treatment on the quality of life in these patients.
Temporal stability of an endemic Mexican treefrog

The demographic characteristics of an amphibian population fluctuate independently over time, mainly in response to the temporal variation of environmental factors, especially precipitation and temperature. These temporal fluctuations may contribute to the size of an amphibian population and could be used to determine the current conservation status of a species. During a five year (2004–2008) period, we studied the relative abundance, sex ratio, and age-sex structure of a population of metamorphosed individuals of the endemic treefrog Hyla eximia in Central Mexico. We also studied the species’ relationship with climatic variables such as temperature and precipitation. We found an interannual constant abundance during the study period. However, interannual differences were observed in the population structure by age-sex category (males, females, or juveniles), with decreased abundance of males and juveniles during the rainy months (August–November). The annual abundance of H. eximia was positively correlated with rainfall, but negatively with monthly temperature. We found the sex ratio was male-biased (2:1), except for year 2008. Also, differences in snout-vent length (SVL) were found between years, suggesting changes in recruitment of new individuals. We conclude that variations in abundance, and frequencies by age-sex category, of H. eximia are related to seasonal variations in temperature and precipitation characteristics of temperate zones. However, this temporal stability may suggest that anurans have an unusual capacity to persist even in the face of human-induced habitat change.
A Comparison of the Different Animal Models of Fetal Alcohol Spectrum Disorders and Their Use in Studying Complex Behaviors

Prenatal ethanol exposure (PNEE) has been linked to widespread impairments in brain structure and function. There are a number of animal models that are used to study the structural and functional deficits caused by PNEE, including, but not limited to invertebrates, fish, rodents, and non-human primates. Animal models enable a researcher to control important variables such as the route of ethanol administration, as well as the timing, frequency and amount of ethanol exposure. Each animal model and system of exposure has its place, depending on the research question being undertaken. In this review, we will examine the different routes of ethanol administration and the various animal models of fetal alcohol spectrum disorders (FASD) that are commonly used in research, emphasizing their strengths and limitations. We will also present an up-to-date summary on the effects of prenatal/neonatal ethanol exposure on behavior across the lifespan, focusing on learning and memory, olfaction, social, executive, and motor functions. Special emphasis will be placed where the various animal models best represent deficits observed in the human condition and offer a viable test bed to examine potential therapeutics for human beings with FASD.
Regulation of VCP/p97 demonstrates the critical balance between cell death and epithelial-mesenchymal transition (EMT) downstream of ER stress

Valosin-containing protein (VCP), also called p97, is a AAA+ ATPase that has been shown to be involved in endoplasmic reticulum-associated protein degradation (ERAD), mitochondria quality control and vesicle transport. We and others have previously found that disruption of VCP is sufficient to cause endoplasmic reticulum (ER) stress. We observed that induction of ER stress either following siRNA mediated loss of VCP or inhibition of VCP with eeyarestatin I potently activates an EMT-like state in cells. Interestingly, both ER stress and EMT are reversible events. Further, brief treatment of cells with eeyarestatin I increases EMT markers, and migratory and invasive properties of lung cancer cells. By examining primary lung adenocarcinoma patient samples we find that the VCP locus is heterozygously lost in nearly half of lung adenocarcinomas and VCP protein expression is decreased in nearly all primary lung tumors. Further, primary lung adenocarcinomas have increased ER stress and EMT markers. These observations have potential clinical relevance because increased ER stress and EMT markers are known to contribute to chemoresistance and poor survival of patients with lung adenocarcinoma.
Essential Opposite Roles of ERK and Akt Signaling in Cardiac Steroid-Induced Increase in Heart Contractility

Interaction of cardiac steroids (CS) with the Na+, K+-ATPase elicits, in addition to inhibition of the enzyme’s activity, the activation of intracellular signaling such as extracellular signal-regulated (ERK) and protein kinase B (Akt). We hypothesized that the activities of these pathways are involved in CS-induced increase in heart contractility. This hypothesis was tested using in vivo and ex vivo wild type (WT) and sarcoplasmic reticulum Ca(2+) atpase1a-deficient zebrafish (accordion, acc mutant) experimental model. Heart contractility was measured in vivo and in primary cardiomyocytes in WT zebrafish larvae and acc mutant. Ca2+ transients were determined ex vivo in adult zebrafish hearts. CS dose dependently augmented the force of contraction of larvae heart muscle and cardiomyocytes and increased Ca2+ transients in WT but not in acc mutant. CS in vivo increased the phosphorylation rate of ERK and Akt in the adult zebrafish heart of the two strains. Pretreatment of WT zebrafish larvae or cardiomyocytes with specific MAPK inhibitors completely abolished the CS-induced increase in contractility. On the contrary, pretreatment with Akt inhibitor significantly enhanced the CS-induced increase in heart contractility both in vivo and ex vivo without affecting CS-induced Ca2+ transients. Furthermore, pretreatment of the acc mutant larvae or cardiomyocytes with Akt inhibitor restored the CS-induced increase in heart contractility also without affecting Ca2+ transients. These results support the notion that the activity of MAPK pathway is obligatory for CS-induced increases in heart muscle contractility. Akt activity, on the other hand, plays a negative role, via Ca2+ independent mechanisms, in CS action. These findings point to novel potential pharmacological intervention to increase CS efficacy.
Climatology of Winter Orographic Precipitation over the Subtropical Central Andes and Associated Synoptic and Regional Characteristics

AbstractWinter orographic precipitation over the Andes between 30° and 37°S is examined using precipitation gauges in the mountains and adjacent lowlands. Because of the limited number of precipitation gauges, this paper focuses on the large-scale variation in cross-barrier precipitation and does not take into account the fine ridge–valley scale. The maximum amount of precipitation was observed on the windward slope of the mountain range below the crest, which was twice that observed on the low-windward side between 32.5° and 34°S. Toward the east of the crest, precipitation amounts drop sharply, generating a strong cross-barrier gradient. The rain shadow effect is greater in the north (32°–34.5°S) than in the south (35°–36.5°S) of the low-lee side, which is probably due to more baroclinic activity in southernmost latitudes and a southward decrease in the height of the Andes enabling more spillover precipitation. The effect of the Andes on winter precipitation is so marked that it modifies the precipitati.
Benign calf amyotrophy: clinicopathologic study of 8 patients.

BACKGROUND The benign focal amyotrophy disorders have been described since 1959 for the upper limbs and since 1981 for the lower limbs. The clinicopathologic features have pointed to a restricted and self-limiting form of motor neuron disease. OBJECTIVE To describe the clinical, electromyographic, and muscle histopathologic features in 8 patients with benign calf amyotrophy. DESIGN Retrospective review of patient charts, electromyograms, and muscle histopathology. PATIENTS AND RESULTS Eight patients, aged 37 to 88 years, developed insidiously progressive calf muscle weakness and wasting during 1 to 5 years. The gastrocnemius weakness and wasting were bilateral in 4 patients. Initial progression of symptoms was followed by disease stabilization. None had a history of poliomyelitis or family history of neuromuscular disease. Creatine kinase values were mildly elevated in 5 patients. The electromyographic and muscle histopathologic findings were consistent with a chronic neuropathic disorder. Despite the restricted calf muscle involvement clinically, the electromyographic abnormalities suggested more diffuse lower limb involvement. Further studies, including DNA tests and muscle-based protein studies, excluded several types of inherited neuromuscular disorders. CONCLUSIONS Benign calf amyotrophy is a variant of the benign focal amyotrophy disorders. The etiology for these disorders is unknown. Studies to exclude other causes of calf amyotrophy and careful follow-up examinations to document disease stabilization are necessary to diagnose this uncommon disorder.
Inhalational Botulism in Rhesus Macaques Exposed to Botulinum Neurotoxin Complex Serotypes A1 and B1

ABSTRACT A recombinant botulinum vaccine (rBV A/B) is being developed for protection against inhalational intoxication with botulinum neurotoxin (BoNT) complex serotype A, subtype A1 (BoNT/A1), and BoNT serotype B, subtype B1 (BoNT/B1). A critical component for evaluating rBV A/B efficacy will be the use of animal models in which the pathophysiology and dose-response relationships following aerosol exposure to well-characterized BoNT are thoroughly understood and documented. This study was designed to estimate inhaled 50% lethal doses (LD50) and to estimate 50% lethal exposure concentrations relative to time (LCt50) in rhesus macaques exposed to well-characterized BoNT/A1 and BoNT/B1. During the course of this study, clinical observations, body weights, clinical hematology results, clinical chemistry results, circulating neurotoxin levels, and telemetric parameters were documented to aid in the understanding of disease progression. The inhaled LD50 and LCt50 for BoNT/A1 and BoNT/B1 in rhesus macaques were determined using well-characterized challenge material. Clinical observations were consistent with the recognized pattern of botulism disease progression. A dose response was demonstrated with regard to the onset of these clinical signs for both BoNT/A1 and BoNT/B1. Dose-related changes in physiologic parameters measured by telemetry were also observed. In contrast, notable changes in body weight, hematology, and clinical chemistry parameters were not observed. Circulating levels of BoNT/B1 were detected in animals exposed to the highest levels of BoNT/B1; however, BoNT/A1 was not detected in the circulation at any aerosol exposure level. The rhesus macaque aerosol challenge model will be used for future evaluations of rBV A/B efficacy against inhalational BoNT/A1 and BoNT/B1 intoxication.
Captive breeding of pangolins: current status, problems and future prospects

Abstract Pangolins are unique placental mammals with eight species existing in the world, which have adapted to a highly specialized diet of ants and termites, and are of significance in the control of forest termite disaster. Besides their ecological value, pangolins are extremely important economic animals with the value as medicine and food. At present, illegal hunting and habitat destruction have drastically decreased the wild population of pangolins, pushing them to the edge of extinction. Captive breeding is an important way to protect these species, but because of pangolin’s specialized behaviors and high dependence on natural ecosystem, there still exist many technical barriers to successful captive breeding programs. In this paper, based on the literatures and our practical experience, we reviewed the status and existing problems in captive breeding of pangolins, including four aspects, the naturalistic habitat, dietary husbandry, reproduction and disease control. Some recommendations are presented for effective captive breeding and protection of pangolins.
Work after Welfare Reform and the Well-being of Children

Using data from five waves of the Women’s Employment Survey (WES; 1997-2003), we examine the links between low-income mothers’ employment experiences and the emotional well-being and academic progress of their children. We find robust linkages between several different dimensions of mothers’ employment experiences and child outcomes. The pattern of results is remarkably similar across all of our empirical approaches—including hierarchical random effects models with an unusually extensive set of controls, child fixed effect models, and instrumental variables estimates. First, children exhibit fewer behavior problems when their mothers work and experience job stability (relative to children whose mothers do not work). In contrast, maternal work accompanied by job instability is associated with significantly higher child behavior problems (relative to job stability). Children whose mothers work full-time and/or have fluctuating levels of work hours or irregular schedules also exhibit significantly higher levels of behavior problems. However, full-time work has negative consequences for children only when it is in jobs that offer limited potential for wage growth. Such negative consequences are completely offset when this work experience is in jobs that require the cognitive skills that lead to higher wage growth prospects. Finally, fluctuating levels of work hours are also strongly associated with the probability that the child will repeat a grade or be placed in special education. These results suggest that “welfare reform,” when considered more broadly to include the new landscape of employment for low-income mothers, has imposed some risks to children’s development. JEL classification: J13, J22, I38
Tyrosine Kinase-Dependent Mechanism Induction of L-Selectin Shedding by a Endothelium Under Nonstatic Conditions: Salicylates Inhibit T Cell Adhesion on

Salicylates inhibit T cell adhesion to and transmigration through endothelium by preventing integrin activation induced by contact with endothelial cells. In the present study the effects of aspirin and sodium salicylate on the ﬁrst steps of T cell adhesion have been analyzed in a nonstatic in vitro system. Salicylates partially reduced adhesion to activated endothelium and, in parallel, L-selectin expression on resting T cells by inducing shedding of the molecule without affecting its mRNA transcript. The role of L-selectin down-regulation in reducing T cell adhesion in this system was supported by the fact that aspirin inhibited T cell adhesion also on plastic-immobilized L-selectin ligand or when a 4 integrin-mediated adhesion to endothelium was blocked by speciﬁc mAbs. In addition, preincubation of T cells with inhibitors of L-selectin shedding prevented both functional and phenotypic inhibitory effects of salicylates. The decrease in T cell adhesion and L-selectin expression seems to be dependent on intracellular calcium increase and tyrosine kinase activation, because these effects could be reversed by preincubating salicylate-treated T cells with EGTA, genistein, or tyrphostin. Finally, the infusion of aspirin into healthy volunteers induced down-regulation of L-selectin on circulating T cells. These results suggest that salicylates interfere not only with integrin activation, but also with the L-selectin-mediated ﬁrst steps of T cell binding to endothelium. The Journal of Immunology, 2001, 166: 832–840.
Seizures after intravenous tramadol given as premedication

A 35-year-old, 50-kg female with a history of epilepsy was scheduled for elective breast surgery (fibroadenoma) under general anaesthesia. She was given glycopyrrolate 0.2 mg, ondansetron 4 mg and tramadol 100 mg i.v. as premedication. Within 5 min, she had an acute episode of generalised tonic–clonic seizure that was successfully treated with 75 mg thiopentone i.v. and after 30 min, she was given general anaesthesia with endotracheal intubation. Surgery, intra-operative period, extubation and post-operative period were uneventful. We conclude that tramadol may provoke seizures in patients with epilepsy even within the recommended dose range.
Biodegradation and transport of benzene, toluene, and xylenes in a simulated aquifer: comparison of modelled and experimental results

Both laboratory experiments and numerical modelling were conducted to study the biodegradation and transport of benzene–toluene–xylenes (BTX) in a simulated semi‐confined aquifer. The factors incorporated into the numerical model include advection, hydrodynamic dispersion, adsorption, and biodegradation. The various physico‐chemical parameters required by the numerical model were measured experimentally.
Preference for Symmetry: Only on Mars?

Preference for symmetry is a robust bias found throughout the animal kingdom. In humans, the bias for symmetry has been documented in numerous domains, including faces and visual patterns. The function of this potent aesthetic bias still eludes us, but prominent accounts focus on its role in mate selection and perceptual fluency. Previous studies have shown that both males and females find symmetrical faces to be more attractive, but here we show that the preference for symmetry in neutral stimuli (ie everyday and meaningless visual objects) is, on the other hand, unique to male participants. Our findings indicate that symmetry preference cannot be explained exclusively by perceptual or computational efficiency, because such an account is domain-independent yet females did not show any bias for the objects tested here. Further studies are needed to elucidate the utility of the male preference for visual object symmetry.
Pressurized groundwater outflow experiments and numerical modeling for outflow channels on Mars

The landscape of Mars shows incised channels that often appear abruptly in the landscape, suggesting a groundwater source. However, groundwater outflow processes are unable to explain the reconstructed peak discharges of the largest outflow channels based on their morphology. Therefore, there is a disconnect between groundwater outflow processes and the resulting morphology. Using a combined approach with experiments and numerical modeling, we examine outflow processes that result from pressurized groundwater. We use a large sandbox flume, where we apply a range of groundwater pressures at the base of a layer of sediment. Our experiments show that different pressures result in distinct outflow processes and resulting morphologies. Low groundwater pressure results in seepage, forming a shallow surface lake and a channel when the lake overflows. At intermediate groundwater pressures, fissures form and groundwater flows out more rapidly. At even higher pressures, the groundwater initially collects in a subsurface reservoir that grows due to flexural deformation of the surface. When this reservoir collapses, a large volume of water is released to the surface. We numerically model the ability of these processes to produce floods on Mars and compare the results to discharge estimates based on previous morphological studies. We show that groundwater seepage and fissure outflow are insufficient to explain the formation of large outflow channels from a single event. Instead, formation of a flexure‐induced subsurface reservoir and subsequent collapse generates large floods that can explain the observed morphologies of the largest outflow channels on Mars and their source areas.
Monocular visual odometry: Sparse joint optimisation or dense alternation?

Real-time monocular SLAM is increasingly mature and entering commercial products. However, there is a divide between two techniques providing similar performance. Despite the rise of ‘dense’ and ‘semi-dense’ methods which use large proportions of the pixels in a video stream to estimate motion and structure via alternating estimation, they have not eradicated feature-based methods which use a significantly smaller amount of image information from keypoints and retain a more rigorous joint estimation framework. Dense methods provide more complete scene information, but in this paper we focus on how the amount of information and different optimisation methods affect the accuracy of local motion estimation (monocular visual odometry). This topic becomes particularly relevant after the recent results from a direct sparse system. We propose a new method for fairly comparing the accuracy of SLAM frontends in a common setting. We suggest computational cost models for an overall comparison which indicates that there is relative parity between the approaches at the settings allowed by current serial processors when evaluated under equal conditions.
Soil Creep and the Development of Hillside Slopes

A mathematical theory of erosion on soil-covered slopes is proposed. The model is based on the hypothesis that soil particles subject to the influence of forces normally operative within soil covers execute randomly directed movements of minute extent. The distribution and characteristics of the pore space of the soil aggregate exert a restrictive influence and determine the nature and magnitude of the particle displacements. Macroscopically, the motion of the soil particles induces a rate of flow on the hillside slope proportional to the surface gradient. The model is used to supply a series of illustrations of the humid cycle of erosion.