Miracle-dz/UniFAR-FTUs huggingface dataset:
Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule‐related proteins in neurons

Modulation of the elongation phase of protein synthesis is important for numerous physiological processes in both neurons and other cell types. Elongation is primarily regulated via eukaryotic elongation factor 2 kinase (eEF2K). However, the consequence of altering eEF2K activity on the synthesis of specific proteins is largely unknown. Using both pharmacological and genetic manipulations of eEF2K combined with two protein‐labeling techniques, stable isotope labeling of amino acids in cell culture and bio‐orthogonal non‐canonical amino acid tagging, we identified a subset of proteins whose synthesis is sensitive to inhibition of eEF2K in murine primary cortical neurons. Gene ontology (GO) analyses indicated that processes related to microtubules are particularly sensitive to eEF2K inhibition. Our findings suggest that eEF2K likely contributes to neuronal function by regulating the synthesis of microtubule‐related proteins.
The EcoCyc Database

EcoCyc is an organism-specific pathway/genome database that describes the metabolic and signal-transduction pathways of Escherichia coli, its enzymes, its transport proteins and its mechanisms of transcriptional control of gene expression. EcoCyc is queried using the Pathway Tools graphical user interface, which provides a wide variety of query operations and visualization tools. EcoCyc is available at http://ecocyc.org/.
Observed Incidence of Uveitis Following Certolizumab Pegol Treatment in Patients With Axial Spondyloarthritis

Axial spondyloarthritis (axial SpA) is characterized by inflammation of the spine and sacroiliac joints and can also affect extraarticular sites, with the most common manifestation being uveitis. Here we report the incidence of uveitis flares in axial SpA patients from the RAPID‐axSpA trial, including ankylosing spondylitis (AS) and nonradiographic (nr) axial SpA.
Predicting persistent disease among children who wheeze during early life

This study sought to determine factors influencing the persistence of early life wheezing up to the age of 10 yrs and to create a score identifying those with the highest risk of persistent disease. Children were seen at birth, 1, 2, 4 and 10 yrs in a whole population birth cohort study (n=1,456). Information was collected prospectively on wheeze prevalence and subjects were classified into wheezing phenotypes. Early life genetic and environmental risk factors were recorded and skin-prick testing (SPT) was performed at 4 yrs. Independently significant factors for persisting wheeze were identified at logistic regression and used to create a score for persistence. Wheezing persistence from the first 4 yrs to the age of 10 yrs occurred in 37% of early life wheezers. Independent significance for persistence was associated with asthmatic family history, atopic SPT at 4 yrs and recurrent chest infections at 2 yrs, whilst recurrent nasal symptoms at 1 yr conferred reduced risk. A cumulative risk score using these factors identified wheezing persistence in 83% scoring 4 and transience in 80% scoring 0. Thus, a combination of genetic predisposition, early life atopy and recurrent chest infections favours the persistence of early life wheezing. Risk scores using such knowledge could provide prognostic guidance on the outcome of early wheeze.
The Psychological Consequences of Money

Money has been said to change people's motivation (mainly for the better) and their behavior toward others (mainly for the worse). The results of nine experiments suggest that money brings about a self-sufficient orientation in which people prefer to be free of dependency and dependents. Reminders of money, relative to nonmoney reminders, led to reduced requests for help and reduced helpfulness toward others. Relative to participants primed with neutral concepts, participants primed with money preferred to play alone, work alone, and put more physical distance between themselves and a new acquaintance.
Perception of global gestalt by temporal integration in simultanagnosia

Patients with bilateral parieto‐occipital brain damage may show intact processing of individual objects, while their perception of multiple objects is disturbed at the same time. The deficit is termed ‘simultanagnosia’ and has been discussed in the context of restricted visual working memory and impaired visuo‐spatial attention. Recent observations indicated that the recognition of global shapes can be modulated by the spatial distance between individual objects in patients with simultanagnosia and thus is not an all‐or‐nothing phenomenon depending on spatial continuity. However, grouping mechanisms not only require the spatial integration of visual information, but also involve integration processes over time. The present study investigated motion‐defined integration mechanisms in two patients with simultanagnosia. We applied hierarchical organized stimuli of global objects that consisted of coherently moving dots (‘shape‐from‐motion’). In addition, we tested the patients’ ability to recognize biological motion by presenting characteristic human movements (‘point‐light‐walker’). The data revealed largely preserved perception of biological motion, while the perception of motion‐defined shapes was impaired. Our findings suggest separate mechanisms underlying the recognition of biological motion and shapes defined by coherently moving dots. They thus argue against a restriction in the overall capacity of visual working memory over time as a general explanation for the impaired global shape recognition in patients with simultanagnosia.
A Review extraction of solar cell modelling parameters

Energy from the sun is the best option for electricity generation as it is abundantly available everywhere and sustainable source. Advance of Photovoltaic (PV) technology in recent years has made solar energy one of the practical alternative energy sources available in the energy market. In order to make the PV energy more affordable and cost effective, major focus of the research community and industry is improvement on power efficiency of PV systems. Parameter extraction of solar cell plays a crucial role in the simulation and design calculation of PV system. The circuit model of a solar cell is non-linear and transcendental. Different methods under the categories of analytic, iterative, and evolutionary computational have been proposed to extract the parameters of solar cell in recent years. This paper presents a survey on parameter extraction of solar cell model and its recent advances.
Microalbuminuria : An Out of Sight Risk among Non-Diabetic Hypertensive Cases Residing In A Rural Area of Lahore

Background: Microalbuminuria is an indicator of generalized vascular damage and a marker of cardiovascular complications in hypertensive. Early detection and prevention of microalbuminuria can help to control these related problems. The purpose of this pilot study was to determine urinary albumin creatinine ratio as measure of microalbuminuria in non-diabetic hypertensive patients residing in a rural area of Lahore. Method: It was a cross-sectional study and was conducted in non diabetic hypertensive patients age >18 years, without a history of pre-existing kidney disease. A morning spot urine sample was obtained from each participant. Urinary albumin was estimated using in-house developed EIA kit for microalbuminuria and creatinine was determined using commercially available kit. Urinary albumin creatinine ratio ≤ 30mg/g was considered as normal. Results: It was observed that urinary albumin creatinine ratios among hypertensive cases were considerably higher among hypertensive cases as compared to normotensive controls (p=0.003). The mean±SD of urinary albumin creatinine ratio (UACR) for normotensive controls, pre-hypertension, mild hypertension, moderate hypertension and that of severe hypertension cases were recorded as 13.7±6.7, 8.3±11.2, 15.5±12.6, 23.2±18.5 and 55.1±93.5 respectively. The comparison of UACR among these five stages were also found significant with p-value <0.001. Conclusion: Screening for microalbuminuria should be practiced in routine management of non diabetic hypertensive patients in the basic health units of rural areas. Furthermore awareness program for general population residing in these rural areas should also be conducted to highlight the importance of screening for microalbuminuria among non diabetic hypertensive.
Mapping of a vaccinia host range sequence by insertion into the viral thymidine kinase gene

A vaccinia virus mutant deleted of ca. 18 kilobase pairs at the left-hand end of the genome is unable to multiply on many human cell lines. To determine whether all or some of the deleted sequences were responsible for the host range property, the corresponding region from wild-type DNA was cloned in three pieces into a vaccinia transplacement vector containing the thymidine kinase gene on the HindIII J fragment. The next step was to transfer these pieces to the genome of the host range deletion mutant by in vivo homologous recombination around the thymidine kinase locus. Transfer of one 5.2-kilobase-pair EcoRI fragment was found to restore a wild-type phenotype on the host range mutant, thus demonstrating that only a small portion of the 18-kilobase-pair deletion contains the host range function(s). This result also illustrates that the method initially devised for inserting foreign genes into vaccinia virus DNA is useful for studies of the vaccinia genome.
Fat-Soluble Vitamin Deficiency in Pediatric Patients with Biliary Atresia

Objective To analyze the levels of fat-soluble vitamins (FSVs) in pediatric patients with biliary atresia (BA) before and after the Kasai procedure. Methods Pediatric patients with obstructive jaundice were enrolled in this study. The FSV levels and liver function before, 2 weeks after, and 1, 3, and 6 months after the Kasai procedure were measured. Results FSV deficiency was more obvious in patients with BA than in patients with other cholestatic liver diseases, especially vitamin D deficiency. 25-Hydroxy vitamin D (25-(OH)D) deficiency was more pronounced in younger patients before surgery. The 25-(OH)D level was significantly higher in patients with than without resolution of jaundice 3 months after surgery. At 6 months after surgery, the 25-(OH)D level was abnormally high at 8.76 ng/ml in patients with unresolved jaundice. Conclusions Preoperative FSV deficiency, particularly vitamin D deficiency, is common in patients with BA. 25-(OH)D deficiency is more pronounced in younger children before surgery. Postoperative FSV deficiency was still prevalent as shown by the lower 25-(OH)D levels in patients with BA and unresolved jaundice. This required long-term vitamin AD supplementation for pediatric patients with BA and unresolved jaundice after surgery.
The TRANSPARENT TESTA16 Locus Encodes the ARABIDOPSIS BSISTER MADS Domain Protein and Is Required for Proper Development and Pigmentation of the Seed Coat Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.004127.

Screening for seed pigmentation phenotypes in Arabidopsis led to the isolation of three allelic yellow-seeded mutants, which defined the novel TRANSPARENT TESTA16 (TT16) locus. Cloning of TT16 was performed by T-DNA tagging and confirmed by genetic complementation and sequencing of two mutant alleles. TT16 encodes the ARABIDOPSIS BSISTER (ABS) MADS domain protein. ABS belongs to the recently identified “B-sister” (BS) clade, which contains genes of unknown function that are expressed mainly in female organs. Phylogenetic analyses using a maximum parsimony approach confirmed that TT16/ABS and related proteins form a monophyletic group. TT16/ABS was expressed mainly in the ovule, as are the other members of the BS clade. TT16/ABS is necessary for BANYULS expression and proanthocyanidin accumulation in the endothelium of the seed coat, with the exception of the chalazal-micropylar area. In addition, mutant phenotype and ectopic expression analyses suggested that TT16/ABS also is involved in the specification of endothelial cells. Nevertheless, TT16/ABS apparently is not required for proper ovule function. We report the functional characterization of a member of the BS MADS box gene subfamily, demonstrating its involvement in endothelial cell specification as well as in the increasingly complex genetic control of flavonoid biosynthesis in the Arabidopsis seed coat.
Tailored interventions to introduce influenza vaccination among 6- to 23-month-old children at inner-city health centers.

BACKGROUND Because of high rates of hospitalization for influenza infections among very young children (< 2 years), the Advisory Committee on Immunization Practices initiated a new policy in 2002 that encouraged vaccination of healthy children aged 6 to 23 months against influenza. OBJECTIVE To evaluate the effectiveness of implementing tailored interventions to introduce influenza vaccination of children 6 to 23 months of age in inner-city practices. STUDY DESIGN A before-after trial with historical and concurrent controls was conducted in 6 health centers in low-income urban locations. METHODS Intervention sites were selected, and interventions (directed at 1534 patients who were 6 to 23 months old) were implemented from a menu of strategies. Vaccination rates were measured from medical record reviews. Focus groups of nursing staffs provided evaluative information on strategies. RESULTS Influenza vaccination rates improved significantly at the intervention health centers compared with the control center. Preintervention (2001-2002) rates ranged from 0% to 7.6%, and intervention (2002-2003) rates ranged from 15.2% to 49.2% (P < .001). The number of interventions ranged from 6 to 11. Sites that used more interventions (odds ratio, 1.24; 95% confidence interval, 1.15-1.34) and had staff support of the vaccination effort (odds ratio, 1.91; 95% confidence interval, 1.40-2.60) had higher vaccination rates. CONCLUSIONS Tailored interventions resulted in successful introduction of influenza vaccination of 6- to 23-month-old children in inner-city health centers. More strategies and enthusiastic staff support may result in higher vaccination rates.
Surgery of the ear and the lateral skull base: pitfalls and complications

Surgery of the ear and the lateral skull base is a fascinating, yet challenging field in otorhinolaryngology. A thorough knowledge of the associated complications and pitfalls is indispensable for the surgeon, not only to provide the best possible care to his patients, but also to further improve his surgical skills. Following a summary about general aspects in pre-, intra-and postoperative care of patients with disorders of the ear/lateral skull base, this article covers the most common pitfalls and complications in stapes surgery, cochlear implantation and surgery of vestibular schwannomas and jugulotympanal paragangliomas. Based on these exemplary procedures, basic “dos and don’ts” of skull base surgery are explained, which the reader can easily transfer to other disorders. Special emphasis is laid on functional aspects, such as hearing, balance and facial nerve function. Furthermore, the topics of infection, bleeding, skull base defects, quality of life and indication for revision surgery are discussed. An open communication about complications and pitfalls in ear/lateral skull base surgery among surgeons is a prerequisite for the further advancement of this fascinating field in ENT surgery. This article is meant to be a contribution to this process.
Leaf Water Relations and Sapflow in Eastern Cottonwood (Populus deltoides Bartr.) Trees Planted for Phytoremediation of a Groundwater Pollutant

ABSTRACT Plants that remediate groundwater pollutants may offer a feasible alternative to the traditional and more expensive practices. Because its success depends on water use, this approach requires a complete understanding of species-specific transpiration patterns. The objectives of this study were (1) to quantify tree and stand-level transpiration in two age classes (whips and 1-year-old seedlings) of eastern cottonwoods (Populus deltoides Bartr.), and (2) to determine climatic and physiological driving variables at the Carswell Air Force Base in central Texas, USA. Trichloroethylene (TCE) was detected in shallow (2 to 3 m) groundwater in the early 1980s. Cottonwood whips and 1-year-old potted seedlings were planted in two separate 0.15-ha plantations in spring 1996. Sapflow gauges determined sapflow on 14 to 16 trees in May, June, July, August, and October 1997. Without adjusting for differences in tree size, sapflow rates were greater for 1-year-old trees than whips (peak values were 0.75 and 0.53 kg hr-1 tree-1, respectively). When adjusted for tree size, the pattern reversed, with whips having significantly greater sapflow rates than 1-year-old trees (peak values were 0.053 and 0.045 kg cm-2 hr-1, respectively). Temporal variation (diurnal and seasonal) in sapflow rates was principally related to VPD, solar radiation, and leaf conductance. Extrapolating to the stand and across the growing season, the plantations transpired ~25 cm of water. Early attainment of high levels of transpiration indicates that the stands will transpire considerably more water as leaf area and root exploitation increases with stand development.
Towards Practical Communication in Byzantine-Resistant DHTs

There are several analytical results on distributed hash tables (DHTs) that can tolerate Byzantine faults. Unfortunately, in such systems, operations such as data retrieval and message sending incur significant communication costs. For example, a simple scheme used in many Byzantine fault-tolerant DHT constructions of n nodes requires O(log3n) messages; this is likely impractical for real-world applications. The previous best known message complexity is O(log2n) in expectation. However, the corresponding protocol suffers from prohibitive costs owing to hidden constants in the asymptotic notation and setup costs. In this paper, we focus on reducing the communication costs against a computationally bounded adversary. We employ threshold cryptography and distributed key generation to define two protocols, both of which are more efficient than existing solutions. In comparison, our first protocol is deterministic with O(log2n) message complexity, and our second protocol is randomized with expected O(logn) message complexity. Furthermore, both the hidden constants and setup costs for our protocols are small, and no trusted third party is required. Finally, we present results from microbenchmarks conducted over PlanetLab showing that our protocols are practical for deployment under significant levels of churn and adversarial behavior.
Hotter nests produce smarter young lizards

A hatchling reptile's sex, body size and shape, and locomotor performance can be influenced not only by its genes, but also by the temperature that it experiences during incubation. Can incubation temperature also affect a hatchling's cognitive skills? In the scincid lizard Bassiana duperreyi, higher incubation temperatures enhanced the resultant hatchling's learning performance. Hence, factors such as maternal nest-site selection and climate change affect not only the size, shape and athletic abilities of hatchling reptiles, but also their ability to learn novel tasks.
Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control

Rice and maize are important cereal crops that serve as staple foods, feed, and industrial material in China. Multiple factors constrain the production of both crops, among which insect pests are an important one. Lepidopteran pests cause enormous yield losses for the crops annually. In order to control these pests, China plays an active role in development and application of genetic engineering (GE) to crops, and dozens of GE rice and GE maize lines expressing insecticidal proteins from the soil bacterium Bacillus thuringiensis (Bt) have been developed. Many lines have entered environmental release, field testing, and preproduction testing, and laboratory and field experiments have shown that most of the Bt rice and Bt maize lines developed in China exhibited effective control of major target lepidopteran pests on rice (Chilo suppressalis, Scirpophaga incertulas, and Cnaphalocrocis medinalis) and maize (Ostrinia furnacalis), demonstrating bright prospects for application. However, none of these Bt lines has yet been commercially planted through this writing in 2016. Challenges and perspectives for development and application of Bt rice and maize in China are discussed. This article provides a general context for colleagues to learn about research and development of Bt crops in China, and may shed light on future work in this field.
Block A*: Database-Driven Search with Applications in Any-Angle Path-Planning

We present three new ideas for grid-based path-planning algorithms that improve the search speed and quality of the paths found. First, we introduce a new type of database, the Local Distance Database (LDDB), that contains distances between boundary points of a local neighborhood. Second, an LDDB based algorithm is introduced, called Block A*, that calculates the optimal path between start and goal locations given the local distances stored in the LDDB. Third, our experimental results for any-angle path planning in a wide variety of test domains, including real game maps, show that Block A* is faster than both A* and the previously best grid-based any-angle search algorithm, Theta*.
hsp 70-Dependent Antiviral Immunity against Cytopathic Neuronal Infection by Vesicular Stomatitis Virus

The major inducible 70-kDa heat shock protein (hsp70) protects against measles virus (MeV) neurovirulence in the mouse that is caused by a cell-associated noncytolytic neuronal infection. Protection is type I interferon (IFN) dependent, and we have established a novel axis of antiviral immunity in which hsp70 is released from virus-infected neurons to induce IFNin macrophages. The present work used vesicular stomatitis virus (VSV) to establish the relevance of hsp70-dependent antiviral immunity to fulminant cytopathic neuronal infections. In vitro, hsp70 that was constitutively expressed in mouse neuronal cells caused a modest increase in VSV replication. Infection induced an early extracellular release of hsp70 from viable cells, and the release was progressive, increasing with virus-induced apoptosis and cell lysis. The impact of this VSV-hsp70 interaction on neurovirulence was established in weanling male hsp70 transgenic and nontransgenic mice. Constitutive expression of hsp70 in neurons of transgenic mice enhanced viral clearance from brain and reduced mortality, and it was correlated with enhanced expression of type I IFN mRNA. Nontransgenic mice were also protected against neurovirulence and expressed increased type I IFN mRNA in brain when hsp70 was expressed by a recombinant VSV (rVSV-hsp70), indicating that hsp70 in the virus-infected cell is sufficient for host protection. In vitro data confirmed extracellular release of hsp70 from cells infected with rVSV-hsp70 and also showed that viral replication is not enhanced when hsp70 is expressed in this manner, suggesting that hsp70-mediated protection in vivo is not dependent on stimulatory effects of hsp70 on virus gene expression.