Miracle-dz/UniFAR-FTUs huggingface dataset:
Occurrence and Distribution of 13 Trichothecene Toxins in Naturally Contaminated Maize Plants in Germany

The objective of the present study was to monitor the occurrence and distribution of a spectrum of trichothecene toxins in different parts of maize plants. Therefore maize plants were sampled randomly from 13 fields in southwest Germany and the fractions kernels, cobs, husks, stalks, leaves and rudimentary ears were analyzed for eight A-type and five B-type trichothecenes. Each of the toxins was found in at least three of the total of 78 samples. The study revealed that both A-type and B-type trichothecenes may be present in all parts of the maize plant but may be unevenly distributed. For the contents of deoxynivalenol, 3- and 15-acetyldeoxynivalenol, nivalenol, scirpentriol, 15-monoacetoxyscirpenol, HT-2 and T-2 toxin significant differences (p < 0.05) were found between different parts of the maize plants whereas no significant differences were observed for fusarenon-X, 4,15-diacetoxyscirpenol, neosolaniol, T-2 triol and T-2 tetraol. Up to twelve toxins co-occurring in one sample were detected. As a group B-type trichothecenes dominated over A-type trichothecenes concerning incidences and levels. Contamination was strongest with rudimentary ears based on incidence and mean and maximum contents; mean contents with few exceptions tended towards a higher level than in other fractions with significant (p < 0.05) differences compared to leaves for seven toxins.
High-Affinity Zn2+ Uptake System ZnuABC Is Required for Bacterial Zinc Homeostasis in Intracellular Environments and Contributes to the Virulence of Salmonella enterica

ABSTRACT To investigate the relevance of zinc in host-pathogen interactions, we have constructed Salmonella enterica mutant strains in which the znuA gene, which encodes the periplasmic component of the ZnuABC high-affinity Zn2+ transporter, was deleted. This mutation does not alter the ability of Salmonella to grow in rich media but drastically reduces its ability to multiply in media deprived of zinc. In agreement with this phenotype, ZnuA accumulates only in bacteria cultivated in environments poor in zinc. In spite of the nearly millimolar intracellular concentration of zinc, we have found that znuA is highly expressed in intracellular salmonellae recovered either from cultivated cells or from the spleens of infected mice. We have also observed that znuA mutants are impaired in their ability to grow in Caco-2 epithelial cells and that bacteria starved for zinc display decreased ability to multiply in phagocytes. A dramatic reduction in the pathogenicity of the znuA mutants was observed in Salmonella-susceptible (BALB/c) or Salmonella-resistant (DBA-2) mice infected intraperitoneally or orally. This study shows that the amount of free metals available for bacterial growth within the infected animal is limited, despite the apparent elevated concentration of free metals within cells and in plasma and suggests that Salmonella exploits the ZnuABC zinc transporter to maximize zinc availability in such conditions. These results shed new light on the complex functions of zinc in vertebrate and bacterial physiology and pave the way for a better comprehension of pathogenic mechanisms in Salmonella infections.
National Study of Physician Awareness and Adherence to Cardiovascular Disease Prevention Guidelines

Background—Few data have evaluated physician adherence to cardiovascular disease (CVD) prevention guidelines according to physician specialty or patient characteristics, particularly gender. Methods and Results—An online study of 500 randomly selected physicians (300 primary care physicians, 100 obstetricians/gynecologists, and 100 cardiologists) used a standardized questionnaire to assess awareness of, adoption of, and barriers to national CVD prevention guidelines by specialty. An experimental case study design tested physician accuracy and determinants of CVD risk level assignment and application of guidelines among high-, intermediate-, or low-risk patients. Intermediate-risk women, as assessed by the Framingham risk score, were significantly more likely to be assigned to a lower-risk category by primary care physicians than men with identical risk profiles (P<0.0001), and trends were similar for obstetricians/gynecologists and cardiologists. Assignment of risk level significantly predicted recommendations for lifestyle and preventive pharmacotherapy. After adjustment for risk assignment, the impact of patient gender on preventive care was not significant except for less aspirin (P<0.01) and more weight management recommended (P<0.04) for intermediate-risk women. Physicians did not rate themselves as very effective in their ability to help patients prevent CVD. Fewer than 1 in 5 physicians knew that more women than men die each year from CVD. Conclusions—Perception of risk was the primary factor associated with CVD preventive recommendations. Gender disparities in recommendations for preventive therapy were explained largely by the lower perceived risk despite similar calculated risk for women versus men. Educational interventions for physicians are needed to improve the quality of CVD preventive care and lower morbidity and mortality from CVD for men and women.
Discussion Paper No . 368 Overconfidence and Managers ́ Responsibility Hoarding

Overconfidence is a well-established behavioral phenomenon that involves an overestimation of own capabilities. We introduce a model, in which managers and agents exert effort in a joint production, after the manager decides on the allocation of the tasks. A rational manager tends to delegate the critical task to the agent more often than given by the efficient task allocation. In contrast, an overconfident manager is more likely to hoard responsibility, i.e. to delegate the critical task less often than a rational manager. In fact, a manager with a sufficiently high ability and a moderate degree of overconfidence increases the total welfare by hoarding responsibility and exerting more effort than a rational manager. Finally, we derive the conditions under which responsibility hoarding can persist in an organization, showing that the bias survives as long as the overconfident manager can rationalize the observed output by underestimating the ability of the agent.
Bcl-xL is an Antiapoptotic Regulator for Postnatal CNS Neurons

Bcl-xL is a death-inhibiting member of the Bcl-2/Ced9 family of proteins which either promote or inhibit apoptosis. Gene targeting has revealed that Bcl-xL is required for neuronal survival during brain development; however,Bcl-xL knock-out mice do not survive past embryonic day 13.5, precluding an analysis of Bcl-xLfunction at later stages of development. Bcl-xL expression is maintained at a high level postnatally in the CNS, suggesting that it may also regulate neuron survival in the postnatal period. To explore functions of Bcl-xL related to neuron survival in postnatal life, we generated transgenic mice overexpressing humanBcl-xL under the control of a pan-neuronal promoter. A line that showed strong overexpression in brainstem and a line that showed overexpression in hippocampus and cortex were chosen for analysis. We asked whether overexpression of Bcl-xLinfluences neuronal survival in the postnatal period by studying two injury paradigms that result in massive neuronal apoptosis. In the standard neonatal facial axotomy paradigm, Bcl-xLoverexpression had substantial effects, with survival of 65% of the motor neurons 7 d after axotomy, as opposed to only 15% in nontransgenic littermates. To investigate whether Bcl-xLregulates survival of CNS neurons in the forebrain, we used a hypoxia–ischemia paradigm in neonatal mice. We show here that hypoxia–ischemia leads to substantial apoptosis in the hippocampus and cortex of wild-type neonatal mice. Furthermore, we show that overexpression of Bcl-xL is neuroprotective in this paradigm. We conclude that levels of Bcl-xL in postnatal neurons may be a critical determinant of their susceptibility to apoptosis.
Model order effects on independent vector analysis applied to complex-valued fMRI data

Independent vector analysis (IVA) has exhibited promising applications to complex-valued fMRI data, however model order effects on complex-valued IVA have not yet been studied. As such, we investigate model order effects on IVA using 16 task-based complex-valued fMRI data sets. A noncircular fixed-point complex-valued IVA (non-FIVA) algorithm was utilized. The model orders were varied from 10 to 160. The ICASSO toolbox was modified for selecting the best spatial estimates across all runs to assess the IVA stability. Non-FIVA was compared to a complex-valued independent component analysis (ICA) algorithm as well as to real-valued IVA and ICA algorithms which analyzed magnitude-only fMRI data. The complex-valued analysis detected component splitting at higher model orders, but in a different way from the magnitude-only analysis in that a complete component and its sub-components exist simultaneously. This suggests that the incorporation of phase fMRI data may better preserve the integrity of the larger networks. Good stability was also achieved by non-FIVA with different orders.
Potassium channel types in arterial chemoreceptor cells and their selective modulation by oxygen

Single K+ channel currents were recorded in excised membrane patches from dispersed chemoreceptor cells of the rabbit carotid body under conditions that abolish current flow through Na+ and Ca2+ channels. We have found three classes of voltage-gated K+ channels that differ in their single-channel conductance (gamma), dependence on internal Ca2+ (Ca2+i), and sensitivity to changes in O2 tension (PO2). Ca(2+)- activated K+ channels (KCa channels) with gamma approximately 210 pS in symmetrical K+ solutions were observed when [Ca2+]i was greater than 0.1 microM. Small conductance channels with gamma = 16 pS were not affected by [Ca2+]i and they exhibited slow activation and inactivation time courses. In these two channel types open probability (P(open)) was unaffected when exposed to normoxic (PO2 = 140 mmHg) or hypoxic (PO2 approximately 5-10 mmHg) external solutions. A third channel type (referred to as KO2 channel), having an intermediate gamma(approximately 40 pS), was the most frequently recorded. KO2 channels are steeply voltage dependent and not affected by [Ca2+]i, they inactivate almost completely in less than 500 ms, and their P(open) reversibly decreases upon exposure to low PO2. The effect of low PO2 is voltage dependent, being more pronounced at moderately depolarized voltages. At 0 mV, for example, P(open) diminishes to approximately 40% of the control value. The time course of ensemble current averages of KO2 channels is remarkably similar to that of the O2-sensitive K+ current. In addition, ensemble average and macroscopic K+ currents are affected similarly by low PO2. These observations strongly suggest that KO2 channels are the main contributors to the macroscopic K+ current of glomus cells. The reversible inhibition of KO2 channel activity by low PO2 does not desensitize and is not related to the presence of F-, ATP, and GTP-gamma-S at the internal face of the membrane. These results indicate that KO2 channels confer upon glomus cells their unique chemoreceptor properties and that the O2-K+ channel interaction occurs either directly or through an O2 sensor intrinsic to the plasma membrane closely associated with the channel molecule.
Historical colonization and demography of the Mediterranean damselfish, Chromis chromis

The desiccation of the Mediterranean Sea during the Messinian Salinity Crisis 6.0–5.3 million years ago (Ma), caused a major extinction of the marine ichthyofauna of the Mediterranean. This was followed by an abrupt replenishment of the Mediterranean from the Atlantic after the opening of the Strait of Gibraltar. In this study, we combined demographic and phylogeographic approaches using mitochondrial and nuclear DNA markers to test the alternative hypotheses of where (Atlantic or Mediterranean) and when (before or after the Messinian Salinity Crisis) speciation occurred in the Mediterranean damselfish, Chromis chromis. The closely related geminate transisthmian pair Chromis multilineata and Chromis atrilobata was used as a way of obtaining an internally calibrated molecular clock. We estimated C. chromis speciation timing both by determining the time of divergence between C. chromis and its Atlantic sister species Chromis limbata (0.93–3.26 Ma depending on the molecular marker used, e.g. 1.23–1.39 Ma for the control region), and by determining the time of coalescence for C. chromis based on mitochondrial control region sequences (0.14–0.21 Ma). The time of speciation of C. chromis was always posterior to the replenishment of the Mediterranean basin, after the Messinian Salinity Crisis. Within the Mediterranean, C. chromis population structure and demographic characteristics revealed a genetic break at the Peloponnese, Greece, with directional and eastbound gene flow between western and eastern groups. The eastern group was found to be more recent and with a faster growing population (coalescent time = 0.09–0.13 Ma, growth = 485.3) than the western group (coalescent time = 0.13–0.20 Ma, growth = 325.6). Our data thus suggested a western origin of C. chromis, most likely within the Mediterranean. Low sea water levels during the glacial periods, the hydrographic regime of the Mediterranean and dispersal restriction during the short pelagic larval phase of C. chromis (18–19 days) have probably played an important role in C. chromis historical colonization.
Configurational Information in Face Perception

A new facial composites technique is demonstrated, in which photographs of the top and bottom halves of different familiar faces fuse to form unfamiliar faces when aligned with each other. The perception of a novel configuration in such composite stimuli is sufficiently convincing to interfere with identification of the constituent parts (experiment 1), but this effect disappears when stimuli are inverted (experiment 2). Difficulty in identifying the parts of upright composites is found even for stimuli made from parts of unfamiliar faces that have only ever been encountered as face fragments (experiment 3). An equivalent effect is found for composites made from internal and external facial features of well-known people (experiment 4). These findings demonstrate the importance of configurational information in face perception, and that configurations are only properly perceived in upright faces.
Optimal caching placement for D2D assisted wireless caching networks

In this paper, we devise the optimal caching placement to maximize the offloading probability for a two-tier wireless caching system, where the helpers and a part of users have caching ability. The offloading comes from the local caching, D2D sharing and the helper transmission. In particular, to maximize the offloading probability we reformulate the caching placement problem for users and helpers into a difference of convex (DC) problem which can be effectively solved by DC programming. Moreover, we analyze the two extreme cases where there is only help-tier caching network and only user-tier. Specifically, the placement problem for the helper-tier caching network is reduced to a convex problem, and can be effectively solved by the classical water-filling method. We notice that users and helpers prefer to cache popular contents under low node density and prefer to cache different contents evenly under high node density. Simulation results indicate a great performance gain of the proposed caching placement over existing approaches.
Rate-dependent mixed H2/H∞ filter design for parameter-dependent state delayed LPV systems

We address the design of parameter-dependent mixed H2/H∞ filters for output estimation of linear parameter-varying (LPV) plants that include a time-varying delay. We investigate the conditions to satisfy asymptotic stability, and H2 and H∞ performance requirements in terms of linear matrix inequalities (LMIs). Our synthesis conditions, that provide mixed H2/Hinfin filters, are dependent linearly on the rate of change of the delay. We take the estimation error into account in the performance index and design the filters to minimize the output energy subject to a bound on the L2-gain from the noise to the estimation error. The designed filters are shown to have the capability of tracking the desired outputs of the plant in the presence of external disturbances and time varying delays. We examine two classes of filters: one that is rational and another one that has delay in the filter dynamics and, hence, it is nonrational. It is shown that the time-delayed (nonrational) filter results in reduced conservatism and improved performance. Illustrative examples are used to verify the design methodology and to demonstrate the superiority of using the proposed delayed filter configuration compared to the rational one.
Recovered memories

underlying stressor was present. The recur rence of symptoms upon the school re-open ing, suggests that the precipitating stressor was related to the school. African outbreaks typically involve missionary schools (Ebra him, 1968) or some conflict between students and administrators (Dhadphale & Shaikh, 1983) which may not be readily apparent to outside investigators. They are typified by children dominated by autocratic elders and having little means of redress, with conflict arising from exposure to foreign ideas which challenge traditional beliefs, fostering escape through conversion (Ebrahim, 1968). There are many questions requiring clar ification through interviews with a represen tative sample ofthose affected, and notjust 12 pupils. It is clear from our sample that â€ ̃¿ mass motor hysteria' subsides only after school administrators reduce or eliminate the anxi cry-generating precipitant which typically involves strict academic or religious disci pline. Hence, it is imperative for investigators to provide some ethnographic description of the participants. It is not enough to state that symptoms were attributed by parents to illness or evil spirits, as this is not a case of mass hysteria by proxy (vide Philen et a! , 1989). Ofkey import is the folk belief of those affected, as conversion symptoms are a symbolic representation of an unresolvable conflict. Two episodes bearing a remarkable similarity to that in Ali-Gombe et al's report (laughing in conjunction with abnormal movements) have been recorded. One af fected six schoolgirls aged 11â€”14in France over 18 days (Armainguad, 1879), while the second was a three-day epidemic in Zambia among 125 students aged 16â€”17(Dhadphale & Shaikh, 1982). Both were triggered by rigid educational policies and involved identifiable index cases.
Comparative gene expression in the heads of Drosophila melanogaster and Tribolium castaneum and the segmental affinity of the Drosophila hypopharyngeal lobes

SUMMARY Drosophila melanogaster has long played an important role in debates surrounding insect and arthropod head segmentation. It is surprising, therefore, that one important feature of Drosophila head segmentation has remained controversial: namely the position of the boundary between the intercalary and mandibular segments. The Drosophila embryonic head has a pair of structures lying behind the stomodeum known as the hypopharyngeal lobes. Traditionally they have been seen as part of the intercalary segment. More recent work looking at the position of the lobes relative to various marker genes has been somewhat equivocal: segment polarity gene expression has been used to argue for a mandibular affinity of these lobes, while the expression of the anterior‐most hox gene labial (lab) has supported an intercalary affinity. We have addressed the question of the segmental affinity of the hypopharyngeal lobes by conducting a detailed comparison of gene expression patterns between Drosophila and the red flour beetle Tribolium castaneum, in which the intercalary segment is unambiguously marked out by lab. We demonstrate that there is a large degree of conservation in gene expression patterns between Drosophila and Tribolium, and this argues against an intercalary segment affinity for the hypopharyngeal lobes. The lobes appear to be largely mandibular in origin, although some gene expression attributed to them appears to be associated with the stomodeum. We propose that the difficulties in interpreting the Drosophila head result from a topological shift in the Drosophila embryonic head, associated with the derived process of head involution.
Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea

Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic protein (cja). The partially purified bacteriocin (PPB) containing colicin type 7 of strain 35/44 was prepared and used for characterization. The antibacterial activity of PPB against a total of 17 selected Gram-positive and Gram-negative bacteria was tested. It was found that PPB of strain 35/44 was active against E. coli O157, S. sonnei and S. boydii. The sensitivity of PPB from this strain to proteinase K, trypsin and α-chymotrypsin suggests the proteinaceous nature of these antimicrobial substances. Therefore, this isolated bacterium can be regarded as bacteriocin producing bacteria. The bacteriocin produced by this isolated S. sonnei was heat stable as evidenced by its ability to maintain the activity at 80 °C for 60 min. In addition, it was stable within a wide range of pH (3–9). The molecular weight of colicin type 7 from isolated S. sonnei strain 35/44 analyzed by SDS-PAGE was 54.4 kDa composing of at least five subunits. It is to our knowledge; the first report of Thai patients with diarrhoea that S. sonnei isolated from them contained colicin type 7.
decapentaplegic is required for arrest in G1 phase during Drosophila eye development.

During eye development in Drosophila, cell cycle progression is coordinated with differentiation. Prior to differentiation, cells arrest in G1 phase anterior to and within the morphogenetic furrow. We show that Decapentaplegic (Dpp), a TGF-&bgr; family member, is required to establish this G1 arrest, since Dpp-unresponsive cells located in the anterior half of the morphogenetic furrow show ectopic S phases and ectopic expression of the cell cycle regulators Cyclins A, E and B. Conversely, ubiquitous over-expression of Dpp in the eye imaginal disc transiently inhibits S phase without affecting Cyclin E or Cyclin A abundance. This Dpp-mediated inhibition of S phase occurs independently of the Cyclin A inhibitor Roughex and of the expression of Dacapo, a Cyclin E-Cdk2 inhibitor. Furthermore, Dpp-signaling genes interact genetically with a hypomorphic cyclin E allele. Taken together our results suggest that Dpp acts to induce G1 arrest in the anterior part of the morphogenetic furrow by a novel inhibitory mechanism. In addition, our results provide evidence for a Dpp-independent mechanism that acts in the posterior part of the morphogenetic furrow to maintain G1 arrest.