Correction: Complete Chloroplast Genome Sequence of Poisonous and Medicinal Plant Datura stramonium: Organizations and Implications for Genetic Engineering

Datura stramonium is a widely used poisonous plant with great medicinal and economic value. Its chloroplast (cp) genome is 155,871 bp in length with a typical quadripartite structure of the large (LSC, 86,302 bp) and small (SSC, 18,367 bp) singlecopy regions, separated by a pair of inverted repeats (IRs, 25,601 bp). The genome contains 113 unique genes, including 80 protein-coding genes, 29 tRNAs and four rRNAs. A total of 11 forward, 9 palindromic and 13 tandem repeats were detected in the D. stramonium cp genome. Most simple sequence repeats (SSR) are AT-rich and are less abundant in coding regions than in non-coding regions. Both SSRs and GC content were unevenly distributed in the entire cp genome. All preferred synonymous codons were found to use A/T ending codons. The difference in GC contents of entire genomes and of the three-codon positions suggests that the D. stramonium cp genome might possess different genomic organization, in part due to different mutational pressures. The five most divergent coding regions and four non-coding regions (trnH-psbA, rps4trnS, ndhD-ccsA, and ndhI-ndhG) were identified using whole plastome alignment, which can be used to develop molecular markers for phylogenetics and barcoding studies within the Solanaceae. Phylogenetic analysis based on 68 protein-coding genes supported Datura as a sister to Solanum. This study provides valuable information for phylogenetic and cp genetic engineering studies of this poisonous and medicinal plant. Citation: Yang Y, Yuanye D, Qing L, Jinjian L, Xiwen L, et al. (2014) Complete Chloroplast Genome Sequence of Poisonous and Medicinal Plant Datura stramonium: Organizations and Implications for Genetic Engineering. PLoS ONE 9(11): e110656. doi:10.1371/journal.pone. 0110656 Editor: Zhong-Hua Chen, University of Western Sydney, Australia Received April 23, 2014; Accepted September 24, 2014; Published November 3, 2014 Copyright: 2014 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. The Datura stramonium chloroplast genome file are available from the GenBank database (accession number NC_018117). All genome files are available from the GenBank database (accession numbers: NC_015113, NC_008325, NC_016430, NC_006290, NC_015621, NC_010601, NC_007977, NC_015543, NC_007578, NC_010442, NC_008535, NC_016468, NC_008407, NC_013707, NC_015604, NC_015401, NC_015623, NC_015608, NC_016433, NC_004561, NC_009808, NC_007500, NC_001879, NC_007602, NC_016068, NC_007943, NC_007898, NC_008096, NC_000932, NC_014674, NC_020318, NC_016921, NC_014697, NC_020152, NC_016730, NC_016728, NC_010093, NC_009601, NC_005973, NC_013823, NC_009618) and are listed in Table S1 of the manuscript. Funding: This work was supported by Macau Science and Technology Development Fund (http://www.fdct.gov.mo/) (077/2011/A3, 074/2012/A3), Research committee of University of Macau (http://www.umac.mo/research/research_committee.html) (MYRG208A (Y3-L4)-ICMS11-WYT, MRG012/WYT/2013/ICMS, MRG013/WYT/2013/ICMS) and the National Natural Science Foundation of China (81202860, 81303160). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * Email: xiweijia2004@aliyun.com (LXW); ytwang@umac.mo (WYT) . These authors contributed equally to this work.