Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of various calpain inhibitors in reduction of light scattering, protein precipitation and nuclear cataract in vitro

Purpose. To compare effects of calpain inhibitors on in vitro light-scattering in rat lens soluble protein and calcium-ionophore (A23187)-induced cataract formation in cultured rat lenses. Methods. Rat lens soluble protein was hydrolyzed for 24 hours by activation of endogenous lens calpain. Ten calpain inhibitors were tested in this model at 10 and 25 µM concentration. As an index of protein precipitation, light scattering was measured daily at 405 nm for 8 days. Lens proteins were analyzed by isoelectric-focussing. Subsequently, rat lenses were cultured for 5 days with 10 µM A23187. Calpain inhibitors (SJA6017, MDL28170, AK295 and PD150606), which inhibited light-scattering were tested at 100 µM concentration in this model. Cataract evaluation, isoelectric-focussing and calcium determinations were performed. Results. At 25 µM concentration AK295, SJA6017, E-64, PD-150606 and MDL28170 produced greater than 25% inhibition of light-scattering. Isoelectric-focussing revealed that addition of Ca 2+ produced characteristic crystallin proteolysis and aggregation patterns. AK295, SJA6017, MDL28170 and E64c prevented these changes. Lenses cultured in A23187 exhibited nuclear cataract, elevated calcium and proteolysis and aggregation of crystallins. Co-culture with SJA6017, MDL28170 and E64c reduced A23187-induced nuclear opacities, proteolysis and aggregation of crystallins without affecting increased total calcium. Conclusions. Endogenous calpain-activation model and A23187-induced cataract model can be used sequentially to screen calpain inhibitors for potential anti-cataract activity. Proteolytic changes in lens cortex after exposure to A23187 are also due to calpain activation. AK295, SJA6017 and MDL28170 possess efficacy against calcium-induced models of rodent cataracts. Use of calpain inhibitors represents a promising approach to cataract therapy.
An evaluation of alertness training for older adults

We present an evaluation of a self-administered, biofeedback-aided, alertness training programme called the Alertness: Training for Focused Living (ATFL) Programme, which was developed as part of the Technology Research for Independent Living (TRIL) collaboration. We conducted two studies in order to evaluate the programme. A randomized controlled trial was, first of all, conducted with 40 older adults aged between 60 and 83. A series of five single case studies was then conducted to examine the suitability of the programme for use with people with more severe memory difficulties. In the randomized controlled trial, participants were assigned to the ATFL Programme or to a placebo programme. Aspects of participants' memory, attention and executive functioning were assessed via telephone prior to and following completion of the training programmes and at 1, 3, and 6-month follow-up sessions. Significant improvements in sustained attention and verbal fluency were noted in the ATFL group. The series of single case studies illustrated the importance of tailoring a programme to the needs and abilities of the clients in question. The potential benefits of the ATFL programme in terms of periodically boosting alertness and aiding executive functioning are discussed.
Differences that hurt: self-perceived health inequalities in Croatia and the European Union.

AIM To investigate the differences in self-reported health status and access to health care according to different income groups, urbanization level, and regional distribution in Croatia and European Union (EU) countries. METHOD Data for the EU countries were taken from the European Quality of Life Survey database, which examines different aspects of quality of life including health and health care. The survey was conducted in 2003 and covered 28 countries, although not Croatia. The survey in Croatia was conducted in 2006 by the United Nations Development Program Croatia. EU countries were divided into two groups--15 "old" EU member states which joined the EU before May 2004 (EU15) and 10 new member states which joined the EU in May 2004 (NMS). The samples were representative and comprised persons aged 18 and over. Statistical differences in health status and access to health care between categories and groups (income groups, urban-rural divide, and analytical regions in case of Croatia) were tested by chi2 test or analysis of variance. RESULTS Significant differences were found among 4 income quartiles in Croatia and two EU country groups in all indicators: self-perceived health status, satisfaction with health, having long-standing illness or disability, access to health care according to four indicators (distance to the nearest medical facility, delay in getting an appointment, waiting time to see the doctor on the day of the appointment, and the cost of seeing the doctor), and the quality of health services. Higher proportion of the Croatian citizens in the lowest income quartile reported poor health (27.8%) than their counterparts in the EU15 (9.2%) or NMS (18.6%). In Croatia, 26% respondents in the lowest quartile perceived the distance to the nearest medical facility as a very serious problem, compared with 5.4% in the EU15 and 9.4% in the NMS. Rural urban proportion ratio of those who reported poor health was about 80% higher in Croatia than in both EU country groups. Rural-urban proportion ratio of those who reported the cost of seeing the doctor as a very serious problem was almost 2-fold higher than in the two EU country groups. CONCLUSION. Health inequalities were more marked in Croatia than in EU countries, which should be taken into serious consideration in shaping health care reforms in Croatia.
Index forecasting and model selection

In this paper we derive a trading strategy that exploits the informational difference implied by different stock market index construction principles. In order to gain a competitive advantage over other market participants we forecast the indexes one day ahead and subsequently generate buy and sell signals through the trading rule. To illustrate how the system works we apply it to select from those stocks that are included in the Austrian Traded Index (ATX). The forecasting of the indexes is performed on the basis of standard financial econometric techniques and feedforward neural networks. We discuss the importance of parsimonious modeling and the applicability of information criteria for architecture selection in artificial neural networks. Copyright © 2002 John Wiley & Sons, Ltd.
A BMI-based occupational therapy assist suit: asynchronous control by SSVEP

A brain-machine interface (BMI) is an interface technology that uses neurophysiological signals from the brain to control external machines. Recent invasive BMI technologies have succeeded in the asynchronous control of robot arms for a useful series of actions, such as reaching and grasping. In this study, we developed non-invasive BMI technologies aiming to make such useful movements using the subject's own hands by preparing a BMI-based occupational therapy assist suit (BOTAS). We prepared a pre-recorded series of useful actions—a grasping-a-ball movement and a carrying-the-ball movement—and added asynchronous control using steady-state visual evoked potential (SSVEP) signals. A SSVEP signal was used to trigger the grasping-a-ball movement and another SSVEP signal was used to trigger the carrying-the-ball movement. A support vector machine was used to classify EEG signals recorded from the visual cortex (Oz) in real time. Untrained, able-bodied participants (n = 12) operated the system successfully. Classification accuracy and time required for SSVEP detection were ~88% and 3 s, respectively. We further recruited three patients with upper cervical spinal cord injuries (SCIs); they also succeeded in operating the system without training. These data suggest that our BOTAS system is potentially useful in terms of rehabilitation of patients with upper limb disabilities.
Efficient speech recognition using subvector quantization and discrete-mixture HMMs

This paper introduces a new form of observation distributions for hidden Markov models (HMMs), combining subvector quantization and mixtures of discrete distributions. We present efficient training and decoding algorithms for the discrete-mixture HMMs (DMHMMs). Our experimental results in the air-travel information domain show that the high-level of recognition accuracy of continuous mixture-density HMMs (CDHMMs) can be maintained at significantly faster decoding speeds. Moreover, we show that when the same number of mixture components is used in DMHMMs and CDHMMs, the new models exhibit superior recognition performance.
Improved Similarity Measures for Software Clustering

Software clustering is a useful technique to recover architecture of a software system. The results of clustering depend upon choice of entities, features, similarity measures and clustering algorithms. Different similarity measures have been used for determining similarity between entities during the clustering process. In software architecture recovery domain the Jaccard and the Unbiased Ellenberg measures have shown better results than other measures for binary and non-binary features respectively. In this paper we analyze the Russell and Rao measure for binary features to show the conditions under which its performance is expected to be better than that of Jaccard. We also show how our proposed Jaccard-NM measure is suitable for software clustering and propose its counterpart for non-binary features. Experimental results indicate that our proposed Jaccard-NM measure and Russell & Rao measure perform better than Jaccard measure for binary features, while for non-binary features, the proposed Unbiased Ellenberg-NM measure produces results which are closer to the decomposition prepared by experts.
The Evaluation of “Spasticity”

ABSTRACT: Lesions of the upper motor neuron cause: 1. Alterations in segmental reflex activity. For example increased tendon jerks and velocity dependent stretch reflexes ("spasticity"), clonus, the clasp knife response, release of flexion reflexes and extensor plantar reflexes. 2. Impaired ability to activate motoneurons rapidly and selectively. Voluntary movements may also be restrained by co-contraction of antagonists muscles, by segmental reflexes (enhanced during voluntary effort) or by contractures. A combination of these factors may impair overall functional ability. Segmental reflexes, voluntary power and overall functional abilities can be assessed using clinical scoring systems. Recordings of muscle length, tension andEMG offer more objective measures of reflex and voluntary activity and of overall functions such as locomotion, and can separate weakness from co-contraction, spasticity from contracture. Methods are now available for exploring individual (transmitter specific) segmental reflex pathways and descending pathways in man. Lesions of the upper motor neuron are complicated by secondary changes in segmental neurons. Segmental reflex activity and muscle mechanics depend on the immediate past history of events. These factors must be taken into account.
Volatile Exchange between Undamaged Plants - a New Mechanism Affecting Insect Orientation in Intercropping

Changes in plant volatile emission can be induced by exposure to volatiles from neighbouring insect-attacked plants. However, plants are also exposed to volatiles from unattacked neighbours, and the consequences of this have not been explored. We investigated whether volatile exchange between undamaged plants affects volatile emission and plant-insect interaction. Consistently greater quantities of two terpenoids were found in the headspace of potato previously exposed to volatiles from undamaged onion plants identified by mass spectrometry. Using live plants and synthetic blends mimicking exposed and unexposed potato, we tested the olfactory response of winged aphids, Myzus persicae. The altered potato volatile profile deterred aphids in laboratory experiments. Further, we show that growing potato together with onion in the field reduces the abundance of winged, host-seeking aphids. Our study broadens the ecological significance of the phenomenon; volatiles carry not only information on whether or not neighbouring plants are under attack, but also information on the emitter plants themselves. In this way responding plants could obtain information on whether the neighbouring plant is a competitive threat and can accordingly adjust their growth towards it. We interpret this as a response in the process of adaptation towards neighbouring plants. Furthermore, these physiological changes in the responding plants have significant ecological impact, as behaviour of aphids was affected. Since herbivore host plants are potentially under constant exposure to these volatiles, our study has major implications for the understanding of how mechanisms within plant communities affect insects. This knowledge could be used to improve plant protection and increase scientific understanding of communication between plants and its impact on other organisms.
DFP‐sensitive multicatalytic protease complexes (proteasomes) involved in the control of oocyte maturation in the toad, Bufo japonicus

The inhibition of progesterone‐induced oocyte maturation by diisopropylfluorophosphate (DFP), a typical serine protease inhibitor, was investigated in oocytes of the Japanese toad Bufo japonicus for the first time. Oocytes to which DFP was externally applied did not undergo germinal vesicle breakdown (GVBD), which is an early signal of oocyte maturation, in response to progesterone. The more inhibitory period was found to be 0–0.5 GVBD50 on a relative time scale [when the time at which 50% of the oocytes had completed GVBD (GVBD50) was set at 1.0], namely, before the beginning of GVBD. DFP‐sensitive proteases, which seem to be multifunctional nonlysosomal protease complexes (proteasomes), may already be present in the cytosol of premature oocytes. Peptide hydrolyzing activity, as reflected by proteasome activity, was found to be regulated before and after GVBD. In addition, immunoblotting regarding the native electrophoretic protein profile of the proteasomes throughout the maturational process demonstrated that they undergo alterations in mobility dependent upon the maturational process.
Nutritional aspects of amino acid metabolism

1. Measurements of the concentrations of amino acids in the plasma of blood from the portal vein and hepatic vein in the livers of rats made diabetic with streptozotocin or alloxan and starved for 1 d were compared with similar measurements from normal rats starved for 1 d. 2. The concentrations of many of the amino acids in the blood plasma were lower in the streptozotocin diabetic rats than in the normal animal while the liver concentrations tended to be increased. This suggests that there is enhanced concentration of these amino acids by the liver in diabetics, as is also found in starvation, which is probably due to factors other than the direct absence of insulin. 3. The direction of flow of the groups of amino acids into and out of the liver was unchanged in the diabetic compared with the normal rat except that the output of tryptophan was abolished, and that of the branched-chain group was abolished in alloxan diabetes though apparently enhanced in streptozotocin diabetes. The rates of movement of amino acids in both directions appeared to be increased in the streptozotocin diabetic animals. 4. The changed amino acid pattern in the alloxan diabetic rats was to some extent similar to that of the streptozotocin diabetic rats but the changes were more difficult to interpret, perhaps because of side-effects of alloxan on tissues including liver.
Technics and Properties of Plywood Manufacture with Modified Urea-Formaldehyde Resin

In this study, urea-formaldehyde resin was modified and used to bond three-ply plywood. The effect of hot press parameters on properties of plywood bonded by the modified urea-formaldehyde resin adhesive was investigated by an L(43) experimental design. The optimum technical parameters of hot press were obtained. Conclusions were as follows: (1) After modification, the free formaldehyde content of the urea-formaldehyde resin and the formaldehyde emission of resulting plywood were reduced by 74% and 70%, respectively; (2) The optimum technical parameters were 140°C of hot-pressing temperature, 80s/mm of hot-pressing time, 300g/m2 of glue content, and 0.8% of curing agent; (3) Under the optimum technical parameters, the bond strength and the formaldehyde emission of the plywood was 1.17MPa and 0.47mg/L, respectively.
Towards New Information Centric Networking Strategy Based on Software Defined Networking

Nowadays, distribution of content, such as video or P2P file sharing, holds the largest share of the Internet traffic. Indeed, the Information Centric Networking architecture (ICN), particularly the Named Data Networking (NDN), appears as a credible alternative to the current Internet model used to deliver content at very large-scale. However, the NDN architecture does not propose a default routing strategy and handles forwarding basically by a network flooding. In this paper, we introduce a Named Data Networking architecture based on Software defined networks (NDNS) to benefit from the advantages of both SDN and NDN. Our proposal relies on the decoupling of the data plane and control plane, to propose a routing strategy ensured by the controller. Moreover, our architecture defines a cache replacement policy, based on the statistical data, in the switch and enables the network management and control service definition. Through experimental tests, we evaluate the performance of our solution and we compare it to classical NDN.
Are there morphological and life‐history traits under climate‐dependent differential selection in S Tunesian Diplotaxis harra (Forssk.) Boiss. (Brassicaceae) populations?

Abstract Adaptation of morphological, physiological, or life‐history traits of a plant species to heterogeneous habitats through the process of natural selection is a paramount process in evolutionary biology. We have used a population genomic approach to disentangle selection‐based and demography‐based variation in morphological and life‐history traits in the crucifer Diplotaxis harra (Forssk.) Boiss. (Brassicaceae) encountered in populations along aridity gradients in S Tunisia. We have genotyped 182 individuals from 12 populations of the species ranging from coastal to semidesert habitats using amplified fragment length polymorphism (AFLP) fingerprinting and assessed a range of morphological and life‐history traits from their progeny cultivated under common‐garden conditions. Application of three different statistical approaches for searching AFLP loci under selection allowed us to characterize candidate loci, for which their association with the traits assessed was tested for statistical significance and correlation with climate data. As a key result of this study, we find that only the shape of cauline leaves seems to be under differential selection along the aridity gradient in S Tunisian populations of Diplotaxis harra, while for all other traits studied neutral biogeographical and/or random factors could not be excluded as explanation for the variation observed. The counter‐intuitive finding that plants from populations with more arid habitats produce broader leaves under optimal conditions of cultivation than those from more mesic habitats is interpreted as being ascribable to selection for a higher plasticity in this trait under more unpredictable semidesert conditions compared to the more predictable ones in coastal habitats.
Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma With Reed-Sternberg–Like Cells and Possible Transformation to Hodgkin's Disease: Mediation by Epstein-Barr Virus

The pathogenesis of Reed-Sternberg cells and variants (RS-H cells) found in rare cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) is unknown. We studied 13 such cases by immunohistochemistry and in situ hybridization for identification of Epstein- Barr virus (EBV) RNA. The RS-H cells in five cases expressed the B-lineage marker CD20 and were negative for CD15. In two cases, the RS-H cells showed expression of both CD20 and CD15, whereas in another six cases, the cells were positive for CD15 but negative for CD20. Three of the cases expressing CD15 showed subsequent evidence of disseminated Hodgkin's disease. Regardless of the phenotype or clinical behavior, the RS-H cells in 12 of 13 cases were found to contain EBV RNA by in situ hybridization, but the surrounding neoplastic lymphocytes were invariably negative for EBV RNA. It is suggested that EBV has an important role in the pathogenesis of the RS-H cells in these rare cases.
Effects of Reticuloendotheliosis Virus Infection on Cytokine Production in SPF Chickens

Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely elucidated. In this study, using multiplex branched DNA (bDNA) technology, we identified molecular mediators that participated in the regulation of the immune response during REV infection in chickens. Cytokine and chemokine mRNA expression levels were evaluated in the peripheral blood mononuclear cells (PBMCs). Expression levels of interleukin (IL)-4, IL-10, IL-13 and tumor necrosis factor (TNF)-α were significantly up-regulated while interferon (IFN)-α, IFN-β, IFN-γ, IL-1β，IL-2, IL-3, IL-15, IL-17F, IL-18 and colony-stimulating factor (CSF)-1 were markedly decreased in PBMCs at all stages of infection. Compared with controls, REV infected chickens showed greater expression levels of IL-8 in PBMCs 21 and 28 days post infection. In addition, REV regulates host immunity as a suppressor of T cell proliferative responses. The results in this study will help us to understand the host immune response to virus pathogens.
Better Access, Quality, and Cost for Clinically Complex Veterans with Home‐Based Primary Care

In successfully reducing healthcare expenditures, patient goals must be met and savings differentiated from cost shifting. Although the Department of Veterans Affairs (VA) Home Based Primary Care (HBPC) program for chronically ill individuals has resulted in cost reduction for the VA, it is unknown whether cost reduction results from restricting services or shifting costs to Medicare and whether HBPC meets patient goals. Cost projection using a hierarchical condition category (HCC) model adapted to the VA was used to determine VA plus Medicare projected costs for 9,425 newly enrolled HBPC recipients. Projected annual costs were compared with observed annualized costs before and during HBPC. To assess patient perspectives of care, 31 veterans and caregivers were interviewed from three representative programs. During HBPC, Medicare costs were 10.8% lower than projected, VA plus Medicare costs were 11.7% lower than projected, and combined hospitalizations were 25.5% lower than during the period without HBPC. Patients reported high satisfaction with HBPC team access, education, and continuity of care, which they felt contributed to fewer exacerbations, emergency visits, and hospitalizations. HBPC improves access while reducing hospitalizations and total cost. Medicare is currently testing the HBPC approach through the Independence at Home demonstration.