Miracle-dz/UniFAR-FTUs huggingface dataset:
Measles Virus Attenuation Associated with Transcriptional Impediment and a Few Amino Acid Changes in the Polymerase and Accessory Proteins

ABSTRACT Measles virus (MV) isolated in B95a cells, a marmoset B-cell line, retains full pathogenicity for cynomolgus monkeys, while its derivative obtained by adaptation to the growth in Vero cells, a monkey kidney cell line, loses the pathogenic potential (F. Kobune, H. Sakata, and A. Sugiura, J. Virol. 64:700–705, 1990). Here, we show with a pair of strains, a fresh isolate (9301B) in B95a cells and its Vero cell-adapted form (9301V), that the in vivo attenuation parallels the decrease of replication and syncytium-inducing capabilities in the original B95a cells and that these in vitro phenotypes are attributable to impediment of transcription, which is already obvious at the level of primary transcription catalyzed by the virion-associated RNA polymerase. On the other hand, cell fusion assays detected no functional difference between the glycoproteins of the two viruses. Essentially the same transcriptional impediment with reduced syncytium induction following Vero cell adaptation was found with two other pairs of strains that had been similarly prepared. Nucleotide sequence comparison between the 9301B and 9301V viruses revealed that a few (at most five) amino acid changes, which sporadically took place in the polymerase (L and P proteins) and/or accessory V and C proteins, were responsible for the in vitro and in vivo attenuation through adaptation to growth in Vero cells.
Effects of Polymorphisms in APOA4-APOA5-ZNF259-BUD13 Gene Cluster on Plasma Levels of Triglycerides and Risk of Coronary Heart Disease in a Chinese Han Population

Background/Aim Recent genome-wide association studies have identified several loci influencing lipid levels. The present study focused on the triglycerides (TG)-associated locus, the APOA4-APOA5-ZNF259-BUD13 gene cluster on chromosome 11, to explore the role of genetic variants in this gene cluster in the development of increasing TG levels and coronary heart disease (CHD). Methodology/Principal Findings Six single nucleotide polymorphisms (SNPs), rs4417316, rs651821, rs6589566, rs7396835, rs964184 and rs17119975, in the APOA4-APOA5-ZNF259-BUD13 gene cluster were selected and genotyped in 5374 healthy Chinese subjects. There were strong significant associations between the six SNPs and TG levels (P<1.0×10−8). Moreover, a weighted genotype score was found to be associated with TG levels (P = 3.28×10−13). The frequencies of three common haplotypes were observed to be significantly different between the high TG group and the low TG group (P<0.05). However, no significant effects were found for the SNPs regarding susceptibility to CHD in the Chinese case-control populations. Conclusions/Significance This study highlights the genotypes, genotype scores and haplotypes of the APOA4-APOA5-ZNF259-BUD13 gene cluster that were associated with TG levels in a Chinese population; however, the genetic variants in this gene cluster did not increase the risk of CHD in the Chinese population.
Expander flows, geometric embeddings and graph partitioning

We give a O(√log n)-approximation algorithm for sparsest cut, balanced separator, and graph conductance problems. This improves the O(log n)-approximation of Leighton and Rao (1988). We use a well-known semidefinite relaxation with triangle inequality constraints. Central to our analysis is a geometric theorem about projections of point sets in Rd, whose proof makes essential use of a phenomenon called measure concentration. We also describe an interesting and natural "certificate" for a graph's expansion, by embedding an n-node expander in it with appropriate dilation and congestion. We call this an expander flow.
Positive Youth Development, Life Satisfaction, and Problem Behaviors of Adolescents in Intact and Non-Intact Families in Hong Kong

This study investigated whether Chinese adolescents living in intact and non-intact families differed in their positive development, life satisfaction, and risk behavior. A total of 3,328 Secondary 1 students responded to measures of positive youth development (such as resilience and psychosocial competencies), life satisfaction, and risk behavior (substance abuse, delinquency, Internet addiction, consumption of pornographic materials, self-harm, and behavioral intention to engage in problem behavior). Findings revealed that adolescents growing up in intact families reported higher levels of positive developmental outcomes and life satisfaction as compared with adolescents from non-intact families. Adolescents in non-intact families also reported higher levels of risk behaviors than those growing up in intact families.
Drinking to cope among college students: prevalence, problems and coping processes.

OBJECTIVE To examine four issues regarding college-student drinking to cope: (1) The prevalence of drinking to cope among students in a medium-sized Midwestern state university; (2) the relationships among drinking to cope and alcohol-related outcomes; (3) the use of drinking to cope within the larger context of students' coping processes; and (4) gender differences in drinking to cope. METHOD Data were drawn from a sample of 275 undergraduates (164 women, 104 men, 7 subjects of unreported gender) in a cross-sectional sample. Of the 275 students assessed, data were analyzed from the 260 who reported ever having consumed alcohol. Drinking to cope was examined both as a style and in response to a specific situation identified by the participant. RESULTS Drinking to cope is very common among college students and is related to much higher levels of alcohol consumption, episodes of heavy drinking, and levels of both negative and positive alcohol-related consequences. In examining the relative influence of drinking to cope and other variables on alcohol use, regression analyses indicated that positive alcohol expectancies were strongly related to alcohol use and that, even when considering other variables, situational drinking to cope remained a fairly strong predictor of most of the alcohol-use indicators. Analysis of gender differences suggested that men rely on alcohol to cope more than women do. CONCLUSIONS Drinking to cope is a significant and highly problematic factor in college student alcohol use. Implications for interventions are discussed.
Distributed under Creative Commons Cc-by 4.0 the Role of Macrobiota in Structuring Microbial Communities along Rocky Shores

Rocky shore microbial diversity presents an excellent system to test for microbial habitat specificity or generality, enabling us to decipher how common macrobiota shape microbial community structure. At two coastal locations in the northeast Pacific Ocean, we show that microbial composition was significantly different between inert surfaces, the biogenic surfaces that included rocky shore animals and an alga, and the water column plankton. While all sampled entities had a core of common OTUs, rare OTUs drove differences among biotic and abiotic substrates. For the mussel Mytilus californianus, the shell surface harbored greater alpha diversity compared to internal tissues of the gill and siphon. Strikingly, a 7-year experimental removal of this mussel from tidepools did not significantly alter the microbial community structure of microbes associated with inert surfaces when compared with unma-nipulated tidepools. However, bacterial taxa associated with nitrate reduction had greater relative abundance with mussels present, suggesting an impact of increased animal-derived nitrogen on a subset of microbial metabolism. Because the presence of mussels did not affect the structure and diversity of the microbial community on adjacent inert substrates, microbes in this rocky shore environment may be predominantly affected through direct physical association with macrobiota.
Improving Delivery Ratio and Power Efficiency in Unicast Geographic Routing with a Realistic Physical Layer for Wireless Sensor Networks

In the last few years, the amount of work in the field of routing algorithms for WSN has increased significantly. The special characteristics of WSN make a challenge for researchers to design efficient routing protocols. Nevertheless, most of the work done left out of scope an inherent aspect to WSN such as the radio transmission errors. The well-known unit disk graph model has been widely used as a basis for several routing protocol. However as recent experimental research has revealed, real links behave completely different to that model. In this paper we evaluate the problems that arise when considering that messages can be lost and propose a new geographic routing protocol which takes into account the probability of error transmission to achieve a high delivery ratio while reducing the energy consumed to the minimum possible. We present the results of the extensive simulation made using our protocol and compare it against a centralised version and two others previous work. Our protocol, being totally distributed and using only local information, outperforms the previous work and achieves almost the same results as the centralised version
aPKCλ/ι promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6

Understanding the mechanism by which hormone refractory prostate cancer (HRPC) develops remains a major issue. Alterations in HRPC include androgen receptor (AR) changes. In addition, the AR is activated by cytokines such as interleukin-6 (IL-6). Atypical protein kinase C (aPKCλ/ι) has been implicated in the progression of several cancers. Herein, we provide evidence that aPKCλ/ι expression correlates with prostate cancer recurrence. Experiments in vitro and in vivo revealed aPKCλ/ι to be involved in prostate cancer cell growth through secretion of IL-6. Further, aPKCλ/ι activates transcription of the IL-6 gene through NFκB and AP-1. We conclude that aPKCλ/ι promotes the growth of hormone independent prostate cancer cells by stimulating IL-6 production in an autocrine manner. Our findings not only explain the link between aPKCλ/ι and IL-6, implicated in the progression a variety of cancers, but also establish a molecular change involved in the development of HRPC. Further, aPKCλ/ι expression might be a biomarker for prostate cancer progression.
A Performance Comparison Through Benchmarking and Modeling of Three Leading Supercomputers: Blue Gene/L, Red Storm, and Purple

This work provides a performance analysis of three leading supercomputers that have recently been deployed: Purple, Red Storm and Blue Gene/L. Each of these machines is architecturally diverse, with very different performance characteristics. Each contains over 10,000 processors and has a system peak of over 40 Teraflops. We analyze each system using a range of micro-benchmarks which include communication performance as well as quantifying the impact of the operating system. The achievable application performance is compared across the systems. The application performance is confirmed via the use of detailed application models which use the underlying performance characteristics as measured by the micro-benchmarks. We also compare the machines in a realistic production scenario in which each machine is used so as to maximize its memory usage with the applications executed in a weak-scaling mode. The results also help illustrate that achievable performance is not directly related to the peak performance
The Idea of South Asia and the Role of the Middle Class

In the post-colonial world, the countries of South Asia have evolved politically in different ways, amidst internal and regional conflicts, but retained some commonality of institutions and cultures. Since the 1990s, the promise of market-led development and the growth of a middle class, especially in India, have reshaped expectations in a way not seen since the immediate post-colonial period, and provided the prospect of a region that combines its particular approach to governance with common aspirations and achievement of economic well-being – what might be a new “idea of South Asia. ” This paper examines some aspects of the development of the South Asian middle class, their role in economic development, and the potential of the idea that shared middle class aspirations of material consumption can be a regional driving force. The paper argues that, for this potential to be realized, the middle class in South Asia may need to aspire to something more than private affluence in the midst of public squalor. In that case, a new idea of South Asia will require building social capital in ways that will challenge all of the region’s societies. Effective collective action across, but first within, the nations of South Asia will be the true test of whether this potential South Asian identity emerges.
Reaction of tetraethylammonium with the open and closed conformations of the acetylcholine receptor ionic channel complex

The effect of tetraethylammonium (TEA) bromide on the neurally and iontophoretically evoked endplate current (EPC) of frog sartorius muscle was investigated using voltage-clamp and noise analysis techniques, and its binding to the acetylcholine (ACh) receptor ionic channel complex was determined on the electric organ of Torpedo ocellata. TEA (250-500 microM) produced an initial enhancement followed by a slow decline in the amplitude of the endplate potential and EPC, but caused only depression in the amplitude of the miniature endplate potential and current. In normal ringer's solution, the EPC current- voltage relationship was approximately linear, and the decay phase varied exponentially with membrane potential. Upon addition of 50-100 microM TEA, the current-voltage relationship became markedly nonlinear at hyperpolarized command potentials, and with 250-2000 microM TEA, there was an initial linear segment, an intermediate nonlinear segment, and a region of negative conductance. The onset of nonlinearity was dose-dependent, undergoing a 50 mV shift for a 10-fold increase in TEA concentration. The EPC decay phase was shortened by TEA at hyperpolarized but not depolarized potentials, and remained a single expotential function of time at all concentrations and membrane potentials examined. These actions of TEA were found to be independent of the sequence of polarizations, the length of the conditioning pulse, and the level of the initial holding potential. TEA shifted the power spectrum of ACh noise to higher frequencies and produced a significant depression of single channel conductance. The shortening in the mean channel lifetime agreed closely with the decrease in the EPC decay time constant. At the concentrations tested, TEA did not alter the EPC reversal potential, nor the resting membrane potential, and had little effect on the action potential duration. TEA inhibited the binding of both [3H] ACh (Ki = 200 microM) and [3H]perhydrohistrionicotoxin (Ki = 280 microM) to receptor-rich membranes from the electric organ of Torpedo ocellata, and inhibited the carbamylcholine-activated 22Na+ efflux from these microsacs. It is suggested that TEA reacts with the nicotinic ACh-receptor as well as its ion channel; the voltage- dependent actions are associated with blockade of the ion channel. The results are compatible with a kinetic model in which TEA first binds to the closed conformation of the receptor-ionicchannel complex to produce a voltage-depdndent depression of endplate conductance and sudsequently to its open conformation, giving rise to the shortening in the EPC decay and mean channel lifetime.
Principals' and Teachers' Perceptions of Learning Disabilities: A Study from NARA Prefecture, Japan

In this study, perceptions of learning disabilities were obtained from 128 principals and 123 teachers in the Nara Prefecture, Japan. A factor analysis indicated that five factors underlie perceptions of learning disabilities: changes in the family and social situation, insufficient knowledge of and support for learning disabilities, teachers' abilities and professional development, teachers' situation and governmental issues. Teachers' situation was perceived to be the main factor, whereas the least important factor was governmental issues. Teachers mainly indicated agreement on the factor of insufficient knowledge of and support for students with learning disabilities. Principals were more aware of governmental issues than teachers.
Lymphocyte Adhesion to Candida albicans

ABSTRACT Adherence of lymphocytes to the fungus is the first step in the direct lymphocyte-mediated antifungal effect against Candida albicans. In this study we identified macrophage-1 antigen (Mac-1) (CD11b/CD18, αM/β2) as the lymphocyte surface structure responsible for the adhesion of activated lymphocytes to the hyphal form of the fungus. Antibodies specific for epitopes of the α-subunit (CD11b) and the β2-subunit (CD18) of Mac-1 were shown to completely eliminate lymphocyte adhesion to C. albicans hyphae. Lymphocyte adhesion to C. albicans was also inhibited significantly by known ligands of Mac-1, including the extracellular matrix proteins laminin and fibrinogen, as well as engineered peptides containing arginine-glycine-aspartic acid sequences and the disintegrin echistatin. N-Acetyl-d-glucosamine and β-glucan, which inhibit Mac-1-mediated adhesion to the yeast, blocked lymphocyte adhesion to hyphae. NIH 3T3 fibroblast transfectants expressing human CD11b/CD18 bound to C. albicans, and their binding was inhibited by antibodies specific for CD11b/CD18. Finally, antibodies specific for CD11b/CD18 effectively inhibited the capacity of activated lymphocytes to have an antifungal effect against hyphae. Our results clearly identify Mac-1 (CD11b/CD18) as the lymphocyte surface structure that mediates activated lymphocyte adhesion to C. albicans and the resultant antifungal effect of the lymphocytes.
IL-4 and TGF-β1 Counterbalance One Another while Regulating Mast Cell Homeostasis

Mast cell responses can be altered by cytokines, including those secreted by Th2 and regulatory T cells (Treg). Given the important role of mast cells in Th2-mediated inflammation and recent demonstrations of Treg-mast cell interactions, we examined the ability of IL-4 and TGF-β1 to regulate mast cell homeostasis. Using in vitro and in vivo studies of mouse and human mast cells, we demonstrate that IL-4 suppresses TGF-β1 receptor expression and signaling, and vice versa. In vitro studies demonstrated that IL-4 and TGF-β1 had balancing effects on mast cell survival, migration, and FcεRI expression, with each cytokine cancelling the effects of the other. However, in vivo analysis of peritoneal inflammation during Nippostrongylus brasiliensis infection in mice revealed a dominant suppressive function for TGF-β1. These data support the existence of a cytokine network involving the Th2 cytokine IL-4 and the Treg cytokine TGF-β1 that can regulate mast cell homeostasis. Dysregulation of this balance may impact allergic disease and be amenable to targeted therapy.
Has Consumption Inequality Mirrored Income Inequality?

We revisit to what extent the increase in income inequality over the last 30 years has been mirrored by consumption inequality. We do so by constructing two alternative measures of consumption expenditure, using data from the Consumer Expenditure Survey (CE). We first use reports of active savings and after tax income to construct the measure of consumption implied by the budget constraint. We find that the consumption inequality implied by savings behavior largely tracks income inequality between 1980 and 2007. Second, we use a demand system to correct for systematic measurement error in the CE's expenditure data. Specifically, we consider trends in the relative expenditure of high income and low income households for different goods with different income (total expenditure) elasticities. Our estimation exploits the difference in the growth rate of luxury consumption inequality versus necessity consumption inequality. This "double-differencing,'' which we implement in a a regression framework, corrects for mis-measurement that can systematically vary over time by good and income group. This second exercise indicates that consumption inequality has closely tracked income inequality over the period 1980-2007. Both of our measures show a significantly greater increase in consumption inequality than what is obtained from the CE's total household expenditure data directly.
Investigation of the ISPP dynamics and of the programming efficiency of charge-trap memories

This paper presents a detailed investigation of the ISPP dynamics of charge-trap memory capacitors, considering not only the flat-band voltage but also the bottom oxide electric field and tunneling current evolution during programming. Differently from the floating-gate case, results on nitride-based memories show that the flat-band increase per step does not equal the step amplitude of the gate staircase, decreasing, moreover, as programming proceeds. As a consequence, the electric field and tunneling current through the bottom oxide are shown to largely increase. Using results at different temperatures and on samples with different stack compositions, this dynamics is explained in terms of a drop of the programming efficiency as more and more charge is stored in the nitride layer, due to the reduction of the number of free traps available for capturing the injected electrons.
Improved detection of Listeria monocytogenes in soft mould‐ripened cheese

In comparison with standard methods, enrichment in half‐Fraser broth for 24 h at 30 °C, followed by plating out onto Listeria monocytogenes blood agar (LMBA) and PALCAM medium combined with an additional streak proved to be the most rapid and specific method for the detection of indigenous L. monocytogenes populations from soft mould‐ripened cheese. This procedure, with a high sensitivity (93%) and a low detection limit (1–10 cfu 25 g−1), provided negative and presumptive positive results within 2–3 d. Differences between LMBA, PALCAM and Oxford medium turned out to be highly significant (at 99% significance level); plating on LMBA after standard enrichment protocols giving the best overall results. An improvement in detection was also obtained by modifying the confirmation procedure. A loopful of culture (an additional streak) from PALCAM or Oxford medium was streaked on non‐selective medium in addition to streaking only separate colonies as specified in the standards.
Cross-Task Compatibility and Age-Related Dual-Task Performance

Background/Study Context: This study examined the effects of stimulus-stimulus and response-response cross-task compatibility and aging on dual-task performance. Hypothesis 1 predicted that the response code compatibility effect in both experiments would benefit older adults comparably to younger adults. Hypothesis 2 predicted that stimulus-stimulus compatibility would be additive to the effects of cross-task compatibility. Methods: Younger and older adults participated in two dual-task experiments. Experiment 1 utilized a cross-task compatibility design identical to that of Koch and Prinz (2002, Journal of Experimental Psychology: Human Perception and Performance, 28, 192–201). Experiment 2 added a stimulus-stimulus compatibility condition. Results: The results of both experiments supported Hypothesis 1. Older adults displayed comparable cross-task compatibility effects to younger adults. The data did not support Hypothesis 2. The response-response compatibility effect from Experiment 1 was replicated, but the effect of stimulus-stimulus compatibility was not significant. Conclusion: The results of both experiments showed that older adults were able to take advantage of cross-task compatibility as a task design to improve dual-task performance. The lack of stimulus-stimulus compatibility effects in Experiment 2 suggested that the benefit of task design may be limited in effect.