Miracle-dz/UniFAR-FTUs huggingface dataset:
Cigarette Smoke Induces C/EBP-β-Mediated Activation of miR-31 in Normal Human Respiratory Epithelia and Lung Cancer Cells

Background Limited information is available regarding mechanisms by which miRNAs contribute to pulmonary carcinogenesis. The present study was undertaken to examine expression and function of miRNAs induced by cigarette smoke condensate (CSC) in normal human respiratory epithelia and lung cancer cells. Methodology Micro-array and quantitative RT-PCR (qRT-PCR) techniques were used to assess miRNA and host gene expression in cultured cells, and surgical specimens. Software-guided analysis, RNA cross-link immunoprecipitation (CLIP), 3′ UTR luciferase reporter assays, qRT-PCR, focused super-arrays and western blot techniques were used to identify and confirm targets of miR-31. Chromatin immunoprecipitation (ChIP) techniques were used to evaluate histone marks and transcription factors within the LOC554202 promoter. Cell count and xenograft experiments were used to assess effects of miR-31 on proliferation and tumorigenicity of lung cancer cells. Results CSC significantly increased miR-31 expression and activated LOC554202 in normal respiratory epithelia and lung cancer cells; miR-31 and LOC554202 expression persisted following discontinuation of CSC exposure. miR-31 and LOC554202 expression levels were significantly elevated in lung cancer specimens relative to adjacent normal lung tissues. CLIP and reporter assays demonstrated direct interaction of miR-31 with Dickkopf-1 (Dkk-1) and DACT-3. Over-expression of miR-31 markedly diminished Dkk-1 and DACT3 expression levels in normal respiratory epithelia and lung cancer cells. Knock-down of miR-31 increased Dkk-1 and DACT3 levels, and abrogated CSC-mediated decreases in Dkk-1 and DACT-3 expression. Furthermore, over-expression of miR-31 diminished SFRP1, SFRP4, and WIF-1, and increased Wnt-5a expression. CSC increased H3K4Me3, H3K9/14Ac and C/EBP-β levels within the LOC554202 promoter. Knock-down of C/EBP-β abrogated CSC-mediated activation of LOC554202. Over-expression of miR-31 significantly enhanced proliferation and tumorigenicity of lung cancer cells; knock-down of miR-31 inhibited growth of these cells. Conclusions Cigarette smoke induces expression of miR-31 targeting several antagonists of cancer stem cell signaling in normal respiratory epithelia and lung cancer cells. miR-31 functions as an oncomir during human pulmonary carcinogenesis.
Graded Effects of Social Conformity on Recognition Memory

Previous studies have shown that the opinion of confederates in a group influences recognition memory, but inconsistent results have been obtained concerning the question of whether recognition of items as old and new are affected similarly, possibly because only one or two confederates are present during the recognition phase. Here, we present data from a study where recognition of novel faces was tested in the presence of four confederates. In a long version of this experiment, recognition of items as old and new was similarly affected by group responses. However, in the short version, recognition of old items depended proportionally on the number of correct group responses, while rejection of new items only decreased significantly when all confederates gave an incorrect response. These findings indicate that differential effects of social conformity on recognition of items as old and new occur in situations with an intermediate level of group pressure.
Dynamical evolution of star-forming regions

We model the dynamical evolution of star-forming regions with a wide range of initial properties. We follow the evolution of the regions’ substructure using the Q-parameter, we search for dynamical mass segregation using the ! MSR technique, and we also quantify the evolution of local density around stars as a function of mass using the "LDR method. The amount of dynamical mass segregation measured by ! MSR is generally only significant for subvirial and virialized, substructured regions – which usually evolve to form bound clusters. The "LDR method shows that massive stars attain higher local densities than the median value in all regions, even those that are supervirial and evolve to form (unbound) associations. We also introduce the Q − "LDR plot, which describes the evolution of spatial structure as a function of mass-weighted local density in a star-forming region. Initially dense (>1000 stars pc−2), bound regions always have Q > 1, "LDR > 2 after 5 Myr, whereas dense unbound regions always have Q 2 after 5 Myr. Less dense regions (<100 stars pc−2) do not usually exhibit "LDR > 2 values, and if relatively high local density around massive stars arises purely from dynamics, then the Q − "LDR plot can be used to estimate the initial density of a star-forming region.
Hyperspectral image analysis for measuring ripeness of tomatoes.

The present invention provides transport apparatus for transporting and articulating an article into and out of a housing having limited access, and includes a lifting beam having a curved shape to which is attached a lifting assembly. A hoist device is connected to the lifting assembly for raising and lowering the entire transport apparatus and also includes a worm-drive assembly for moving the lifting assembly relative to the lifting beam to thereby articulate an article about a first axis. In addition, one end of the lifting beam includes a rotational mechanism which is connected to a second worm-drive assembly. Operation of the second worm-drive assembly allows the article to be rotated relative to the lifting beam which allows the article to be rotated relative to a second axis. The combined operations of hoisting and movement about the two axes allows the article to be articulated in any direction for insertion or removal from a housing having limited access.
Towards a solution of the state-space realization problem of a set of multi-input multi-output nonlinear difference equations

The paper deals with nonlinear system described by the set of higher-order difference equations in the inputs and the outputs. A theorem for transforming such a system around a regular equilibrium point into a locally equivalent one, but having the so-called row- and column-reduced form, is formulated. The importance of this form comes from the fact that this is a suitable starting point for studing the realization problem for a set of nonlinear multi-input multi-output difference equations.
Agreement in Self–Other Ratings of Leader Effectiveness: The Role of Demographics and Personality

Personality and demographic attributes for a set of 1221 focal managers were examined as correlates of leadership effectiveness evaluations that were obtained via a 360° feedback program. Polynomial regression was used to study the congruence of self-ratings provided by focal managers relative to the different evaluative perspectives (i.e., immediate superior, peer, and subordinate). Analyses supported the prediction that focal manager's sex and age would be associated with the ratings provided by themselves and others. Plus, the tendency to overestimate one's own leader effectiveness relative to evaluations provided by others was found to be greater for males and older managers. Focal managers who expressed greater social sensitivity were evaluated more favorably by subordinates and peers, although not by superiors. Ratings of leader effectiveness from immediate superiors were, instead, more readily predicted by judgments of the performance of the focal manager's organizational unit relative to comparable units. Results of polynomial regression analysis, however, indicated that self - other agreement was related to the focal's sex, social sensitivity, and social dominance. Implications for understanding obstacles to openness to change are discussed.
Smoking is a risk factor for early conversion to clinically definite multiple sclerosis

Background Cigarette smoking increases the risk for development of multiple sclerosis and modifies the clinical course of the disease. In this study, we determined whether smoking is a risk factor for early conversion to clinically definite multiple sclerosis after a clinically isolated syndrome. Methods We included 129 patients with a clinically isolated syndrome, disseminated white-matter lesions on brain magnetic resonance imaging, and positive oligoclonal bands in the cerebrospinal fluid. The patients’ smoking status was obtained at the time of the clinically isolated syndrome. Results During a follow-up time of 36 months, 75% of smokers but only 51% of non-smokers developed clinically definite multiple sclerosis, and smokers had a significantly shorter time interval to their first relapse. The hazard ratio for progression to clinically definite multiple sclerosis was 1.8 (95% confidence interval, 1.2–2.8) for smokers compared with non-smokers (P = 0.008). Conclusions Smoking is associated with an increased risk for early conversion to clinically definite multiple sclerosis after a clinically isolated syndrome, and our results suggest that smoking is an independent but modifiable risk factor for disease progression of multiple sclerosis. Therefore, it should be considered in the counseling of patients with a clinically isolated syndrome.
Reading other minds: Effects of literature on empathy

The potential of literature to increase empathy was investigated in an experiment. Participants (N = 100, 69 women) completed a package of questionnaires that measured lifelong exposure to fiction and nonfiction, personality traits, and affective and cognitive empathy. They read either an essay or a short story that were equivalent in length and complexity, were tested again for cognitive and affective empathy, and were finally given a non-self-report measure of empathy. Participants who read a short story who were also low in Openness experienced significant increases in self-reported cognitive empathy (p .05). No increases in affective empathy were found. Participants who were frequent fiction-readers had higher scores on the non-self-report measure of empathy. Our results suggest a role for fictional literature in facilitating development of empathy.
Creation of a Recombinant Rift Valley Fever Virus with a Two-Segmented Genome

ABSTRACT Rift Valley fever virus (RVFV; family Bunyaviridae) is a clinically important, mosquito-borne pathogen of both livestock and humans, which is found mainly in sub-Saharan Africa and the Arabian Peninsula. RVFV has a trisegmented single-stranded RNA (ssRNA) genome. The L and M segments are negative sense and encode the L protein (viral polymerase) on the L segment and the virion glycoproteins Gn and Gc as well as two other proteins, NSm and 78K, on the M segment. The S segment uses an ambisense coding strategy to express the nucleocapsid protein, N, and the nonstructural protein, NSs. Both the NSs and NSm proteins are dispensable for virus growth in tissue culture. Using reverse genetics, we generated a recombinant virus, designated r2segMP12, containing a two-segmented genome in which the NSs coding sequence was replaced with that for the Gn and Gc precursor. Thus, r2segMP12 lacks an M segment, and although it was attenuated in comparison to the three-segmented parental virus in both mammalian and insect cell cultures, it was genetically stable over multiple passages. We further show that the virus can stably maintain an M-like RNA segment encoding the enhanced green fluorescent protein gene. The implications of these findings for RVFV genome packaging and the potential to develop multivalent live-attenuated vaccines are discussed.
Overexpression of degenerative spermatocyte homolog 1 up‐regulates the expression of cyclin D1 and enhances metastatic efficiency in esophageal carcinoma Eca109 cells

Cyclin D1 plays a pivotal role in cell‐cycle transition through G1 phase. In this article, we found that Degenerative Spermatocyte Homolog 1 (DEGS1) up‐regulated the expression of cyclin D1 and the activation of transcription factor NF‐κB was essential for DEGS1‐induced cyclin D1 production. Forced expression of DEGS1 in Esophageal carcinoma cell line Eca109 cells increased their ability of cell migration and significantly induced tumor metastasis in nude mice, whereas RNA interference‐mediated knockdown of DEGS1 cells significantly inhibited cell migration in vitro, as well as tumor metastasis in vivo. Our results demonstrated that expression of DEGS1 up‐regulated the expression of cyclin D1 and enhanced the efficiency of tumor metastasis. © 2009 Wiley‐Liss, Inc.
Effects of Transmitters and Amyloid-Beta Peptide on Calcium Signals in Rat Cortical Astrocytes: Fura-2AM Measurements and Stochastic Model Simulations

Background To better understand the complex molecular level interactions seen in the pathogenesis of Alzheimer's disease, the results of the wet-lab and clinical studies can be complemented by mathematical models. Astrocytes are known to become reactive in Alzheimer's disease and their ionic equilibrium can be disturbed by interaction of the released and accumulated transmitters, such as serotonin, and peptides, including amyloid- peptides (A). We have here studied the effects of small amounts of A25–35 fragments on the transmitter-induced calcium signals in astrocytes by Fura-2AM fluorescence measurements and running simulations of the detected calcium signals. Methodology/Principal Findings Intracellular calcium signals were measured in cultured rat cortical astrocytes following additions of serotonin and glutamate, or either of these transmitters together with A25–35. A25–35 increased the number of astrocytes responding to glutamate and exceedingly increased the magnitude of the serotonin-induced calcium signals. In addition to A25–35-induced effects, the contribution of intracellular calcium stores to calcium signaling was tested. When using higher stimulus frequency, the subsequent calcium peaks after the initial peak were of lower amplitude. This may indicate inadequate filling of the intracellular calcium stores between the stimuli. In order to reproduce the experimental findings, a stochastic computational model was introduced. The model takes into account the major mechanisms known to be involved in calcium signaling in astrocytes. Model simulations confirm the principal experimental findings and show the variability typical for experimental measurements. Conclusions/Significance Nanomolar A25–35 alone does not cause persistent change in the basal level of calcium in astrocytes. However, even small amounts of A25–35, together with transmitters, can have substantial synergistic effects on intracellular calcium signals. Computational modeling further helps in understanding the mechanisms associated with intracellular calcium oscillations. Modeling the mechanisms is important, as astrocytes have an essential role in regulating the neuronal microenvironment of the central nervous system.
Blind adaptive multiuser detection for asynchronous dual-rate DS/CDMA systems

In this paper, the authors consider an asynchronous direct-sequence code division multiple access (DS/CDMA) system wherein users are allowed to transmit their symbols at one out of two available data rates. Three possible access schemes are considered, namely, the variable spreading length (VSL), the variable chip rate (VCR), and the variable chip rate with frequency shift (VCRFS) formats. Their performance is compared for the case that a linear one-shot multiuser receiver is employed. It is also shown that detection of the users transmitting at the higher rate requires a periodically time-varying processing of the observables. Moreover, the problem of blind adaptive receiver implementation is studied, and a cyclic blind recursive-least-squares (RLS) algorithm is provided which is capable of converging to the periodically time-varying high-rate users detection structure. Numerical results show that the proposed receivers are near-far resistant, and that the VCRFS access technique achieves the best performance. Finally as to the adaptive blind receiver implementation, computer simulations have revealed that the cyclic RLS algorithm for blind adaptive high-rate users demodulation outperforms the conventional RLS algorithm in most cases of primary importance.
Using the Stochastic Multicloud Model to Improve Tropical Convective Parameterization: A Paradigm Example

AbstractDespite recent advances in supercomputing, current general circulation models (GCMs) poorly represent the variability associated with organized tropical convection. A stochastic multicloud convective parameterization based on three cloud types (congestus, deep, and stratiform), introduced recently by Khouider, Biello, and Majda in the context of a single column model, is used here to study flows above the equator without rotation effects. The stochastic model dramatically improves the variability of tropical convection compared to the conventional moderate- and coarse-resolution paradigm GCM parameterizations. This increase in variability comes from intermittent coherent structures such as synoptic and mesoscale convective systems, analogs of squall lines and convectively coupled waves seen in nature whose representation is improved by the stochastic parameterization. Furthermore, simulations with a sea surface temperature (SST) gradient yield realistic mean Walker cell circulation with plausible .
Health-seeking behaviours by gender among adolescents in Soweto, South Africa

Background Adolescents are an important age-group for preventing disease and supporting health yet little is known about their health-seeking behaviours. Objective We describe socio-demographic characteristics and health-seeking behaviours of adolescents in Soweto, South Africa, in order to broaden our understanding of their health needs. Design The Botsha Bophelo Adolescent Health Study was an interviewer-administered cross-sectional survey of 830 adolescents (14–19 years) conducted in Soweto from 2010 to 2012. Health-seeking behaviours were defined as accessing medical services and/or being hospitalised in the 6 months prior to the survey. Chi-square analysis tested for associations between gender, other socio-demographic and behavioural characteristics, and health-seeking behaviours. Results Of 830 adolescents, 57% were female, 50% were aged 17–19 years, 85% were enrolled in school, and 78% reported experiencing medium or high food insecurity. Males were more likely than females to report sexual debut (64% vs. 49%; p<0.0001) and illicit drug use (11% vs. 3%; p<0.0001). Approximately 27% (n=224) and 8% (n=65) reported seeking healthcare or being hospitalised respectively in the previous 6 months, with no significant differences by gender. Services were most commonly sought at medical clinics (75%), predominantly because of flu-like symptoms (32%), followed by concerns about HIV (10%). Compared to females, males were more likely to seek healthcare for condom breakage (8% vs. 2%; p=0.02). Relative to males, a significantly higher proportion of females desired general healthcare services (85% vs. 78%; p=0.0091), counselling (82% vs. 70%; p<0.0001), and reproductive health services (64% vs. 56%; p=0.02). Conclusions A quarter of male and female adolescents accessed health services in the 6 months prior to the interview. Adolescents reported a gap between the availability and the need for general, reproductive, and counselling services. Integrated adolescent-friendly, school-based health services are recommended to bridge this gap.
Dengue virus infection elicits highly polarized CX3CR1+ cytotoxic CD4+ T cells associated with protective immunity

Significance Infections with any of the four dengue virus serotypes (DENV 1–4) are the most prevalent and rapidly spreading mosquito-borne viral infections in humans. There is no treatment or vaccine currently available. We found that the virus-specific cells display a highly polarized cytotoxic phenotype that correlated with expression of a protective HLA DR allele. Although the occurrence of cytotoxic CD4+ T cells in humans has been described in the context of some chronic viral infections, to our knowledge, this is the first report of ex vivo cytotoxic CD4+ activity after exposure with an acute virus. These results will help shed light on the specific role of CD4+ T cells in DENV infection and may help in finding a correlate of protection. Dengue virus (DENV) is a rapidly spreading pathogen with unusual pathogenesis, and correlates of protection from severe dengue disease and vaccine efficacy have not yet been established. Although DENV-specific CD8+ T-cell responses have been extensively studied, the breadth and specificity of CD4+ T-cell responses remains to be defined. Here we define HLA-restricted CD4+ T-cell epitopes resulting from natural infection with dengue virus in a hyperepidemic setting. Ex vivo flow-cytometric analysis of DENV-specific CD4+ T cells revealed that the virus-specific cells were highly polarized, with a strong bias toward a CX3CR1+ Eomesodermin+ perforin+ granzyme B+ CD45RA+ CD4 CTL phenotype. Importantly, these cells correlated with a protective HLA DR allele, and we demonstrate that these cells have direct ex vivo DENV-specific cytolytic activity. We speculate that cytotoxic dengue-specific CD4+ T cells may play a role in the control of dengue infection in vivo, and this immune correlate may be a key target for dengue virus vaccine development.
Structural, functional and molecular dynamics analysis of the native and mutated actin to study its effect on congenital myopathy

Structural, functional and molecular dynamics analysis of the native and mutated actin to study its effect on congenital myopathy Faeze Sadat Mohajer, Sepideh Parvizpour, Jafar Razmara, Mahsa Khoshkhooy Yazdi and Mohd Shahir Shamsir* Bioinformatics Research Group, Faculty of Bioscience and Medical Engineering, UniversitiTeknologi Malaysia, Johor Bahru, Malaysia; Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Departement of Computer Science, University of Tabriz, Tabriz, Iran; Faculty of Science, Department of Chemistry, Universiti Teknologi Malaysia, 81310 UTM, Johor, Malaysia
Annals of the New York Academy of Sciences What Does the Amygdala Contribute to Social Cognition?

The amygdala has received intense recent attention from neuroscientists investigating its function at the molecular, cellular, systems, cognitive, and clinical level. It clearly contributes to processing emotionally and socially relevant information, yet a unifying description and computational account have been lacking. The difficulty of tying together the various studies stems in part from the sheer diversity of approaches and species studied, in part from the amygdala's inherent heterogeneity in terms of its component nuclei, and in part because different investigators have simply been interested in different topics. Yet, a synthesis now seems close at hand in combining new results from social neuroscience with data from neuroeconomics and reward learning. The amygdala processes a psychological stimulus dimension related to saliency or relevance; mechanisms have been identified to link it to processing unpredictability; and insights from reward learning have situated it within a network of structures that include the prefrontal cortex and the ventral striatum in processing the current value of stimuli. These aspects help to clarify the amygdala's contributions to recognizing emotion from faces, to social behavior toward conspecifics, and to reward learning and instrumental behavior.