Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Anticonvulsant Drugs on Thyroid Hormones in Epileptic Children

Long‐term therapy with valproate, phenytoin (diphenylhydantoin, DPH), primidone, carbamazepine, and combinations of these produced considerable alterations in the thyroid hormonal state in epileptic children (n = 125). These consist of decreases in protein‐bound iodine (PBI), thyroxin (T4), free T4, triiodothyronine (T3), and to a lesser extent in thyrotropin hormone (TSH). In carbamazepine and antiepileptic combination therapy, statistically significant increases of total cholesterol were confirmed. The changes described are dose dependent for valproate, DPH, and carbamazepine. They are based on a displacement of thyroxin and T3 from their protein‐binding sites, as well as on a more rapid conversion and metabolism of T4 and T3. In the TRH test, marked TSH stimulation resulted in 8% of our 50 tested patients, suggesting a hypothyroid state. The lack of increase of basic TSH in the majority of the treated children could result from a hypothalamic effect of the anticonvulsant drugs that might result in a TRH inhibition. Furthermore, in a greater number of the treated children it must be assumed that a latent form of hypothyroidism exists.
Evaluation of Tomato Cultivars for Yield, Profitand Quality Performance in an Organic Management System in North Western Himalayas, India

Tomato is grown worldwide for its edible fruits, with antioxidants benefits. It has been reported that consumption of raw tomato and tomato based products is associated with reduced risk of cancer and cardiovascular disease (Giovannucci et al., 2002). The organic production system aims at supporting and sustaining healthy eating habits, International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 10 (2018) Journal homepage: http://www.ijcmas. com
Fourier analysis of saccades in monkeys and humans.

1. By recording eye movements with the search coil technique and subjecting them to an accurate infinite Fourier transform algorithm, we describe the Fourier spectra of human and monkey saccades. In both species we find heretofore undescribed features consisting of a regular pattern of local minima in the power plot, which cannot be attributed to noise. The frequency of these minima is well correlated with saccade duration. 2. Computer simulation shows that if the pulse component of the saccade is considered to be rectangular, then the first of these minima (called M1) occurs at a frequency that is the reciprocal of the duration of the pulse. 3. Comparing the position of this component during individual monkey saccades with electrophysiological recordings of motoneurons during the same saccades leads to the conclusion that these minima are related to the burst components in ocular motoneuron discharges. Specifically, the reciprocals of the frequencies of these minima are correlated with the duration of the burst component in the motoneuron discharge. 4. In the Fourier spectra of human saccades, the relationship of the frequency of M1 to saccadic duration is a function similar to that in the monkey. This adds to the evidence that the human saccade also is driven by a pulse-step signal. 5. In both monkeys and humans, T1, the reciprocal of the frequency of M1, is shorter than both the saccade duration and the burst duration of individual motoneurons, even though neurophysiological studies in monkeys generally report the saccadic burst duration to be equal to the saccade duration. This probably arises because the saccadic pulse is not rectangular, with the extremes contributing very little energy to the Fourier spectrum. By further computer modeling we show these shape effects explicitly: as the rise- and falltime increase, making the pulse less rectangular, T1 becomes shorter; in addition, as the asymmetry of rise and fall increases, the depth of the minima is reduced. We conclude that T1 measures the "effective pulse" duration of the motoneuron. 6. There is a difference in the relationship of effective pulse duration to the saccade duration between short and long saccades. For saccades shorter than approximately 40 ms in the human and 50 ms in the monkey, the pulse width as measured by this technique varies little with saccade duration. For longer saccades, effective pulse width increases linearly with duration. We agree with others that for short saccades the pulse is both height- and width-modulated; but for longer saccades, height modulation saturates and only width modulation remains. (ABSTRACT TRUNCATED AT 400 WORDS)
Impact of land convection on troposphere-stratosphere exchange in the tropics

The mechanism of troposphere-stratosphere exchange in the tropics was investigated from space-borne observations of the horizontal distributions of tropospheric-origin long-lived species, nitrous oxide (N2O), methane (CH4) and carbon monoxide (CO), from 150 to 70 hPa in March-April-May by the ODIN/Sub-Millimeter Radiometer (SMR), the Upper Atmosphere Research Satellite (UARS)/Halogen Occultation Experiment (HALOE) and the TERRA/Measurements Of Pollution In The Troposphere (MOPITT) instruments in 2002–2004, completed by recent observations of the AURA/Microwave Limb Sounder (MLS) instrument during the same season in 2005. The vertical resolution of the satellite measurements ranges from 2 to 4 km. The analysis has been performed on isentropic surfaces: 400 K (lower stratosphere) for all the species and 360 K (upper troposphere) only for CO. At 400 K (and 360 K for CO), all gases show significant longitudinal variations with peak-to-trough values of ~5–11 ppbv for N2O, 0.07–0.13 ppmv for CH4, and ~10 ppbv for CO (~40 ppbv at 360 K). The maximum amounts are primarily located over Africa and, depending on the species, secondary more or less pronounced maxima are reported above northern South America and South-East Asia. The lower stratosphere over the Western Pacific deep convective region where the outgoing longwave radiation is the lowest, the tropopause the highest and the coldest, appears as a region of minimum concentration of tropospheric trace species. The possible impact on trace gas concentration at the tropopause of the inhomogeneous distribution and intensity of the sources, mostly continental, of the horizontal and vertical transports in the troposphere, and of cross-tropopause transport was explored with the MOCAGE Chemistry Transport Model. In the simulations, significant longitudinal variations were found on the medium-lived CO (2-month lifetime) with peak-to-trough value of ~20 ppbv at 360 K and ~10 ppbv at 400 K, slightly weaker than observations. However, the CH4 (8–10 year lifetime) and N2O (130-year lifetime) longitudinal variations are significantly weaker than observed: peak-to-trough values of ~0.02 ppmv for CH4 and 1–2 ppbv for N2O at 400 K. The large longitudinal contrast of N2O and CH4 concentrations reported by the space-borne instruments at the tropopause and in the lower stratosphere not captured by the model thus requires another explanation. The suggestion is of strong overshooting over land convective regions, particularly Africa, very consistent with the space-borne Tropical Rainfall Measuring Mission (TRMM) radar maximum overshooting features over the same region during the same season. Compared to observations, the MOCAGE model forced by ECMWF analyses is found to ignore these fast local uplifts, but to overestimate the average uniform vertical transport in the UTLS at all longitudes in the tropics.
Monokine production by hypersensitivity (Schistosoma mansoni egg) and foreign body (Sephadex bead)-type granuloma macrophages. Evidence for sequential production of IL-1 and tumor necrosis factor.

The present study examined the potential role of IL-1 and TNF in granuloma formation. Mice were given i.v. injections of Schistosoma mansoni eggs or Sephadex beads to induce synchronous immune T cell-mediated (hypersensitivity type) or nonimmune (foreign-body type) granulomas, respectively. Granuloma macrophages isolated at 2, 4, 8, 16, and 32 days of granuloma formation were evaluated for their capacity to produce IL-1 and TNF in response to 1 microgram/ml LPS. This was related to circulating levels of the acute phase protein, serum amyloid P (SAP) and expression of Ia Ag by monocytes and macrophages. Macrophages from nonimmune bead lesions were generally weak producers of IL-1 and TNF. In contrast, those from T cell-mediated egg lesions produced significant levels of both monokines. Moreover, there was a clear pattern of sequential monokine production such that IL-1 was produced in greatest amounts early (2 to 4 days), whereas TNF was produced later (8 to 16 days). Levels of SAP showed an initial sharp rise following particle embolization, then decreased rapidly in bead injected animals. However, mice with immune granulomas showed a prolonged elevation in SAP levels that corresponded to the period of maximal IL-1 production (2 to 4 days). Macrophage/monocyte Ia Ag expression was greatest at 8 to 16 days, corresponding to the period of TNF production. Bead injected animals showed low levels of Ia expression over the study period. These findings suggest that IL-1 may be important in the early recruitment stages of granuloma formation while TNF may take part in later maintenance or effector functions. The extent of production of both is likely influenced by the local or systemic milieu of lymphokines.
Domestication and Crop Physiology: Roots of Green-Revolution Wheat

Background and Aims Most plant scientists, in contrast to animal scientists, study only half the organism, namely above-ground stems, leaves, flowers and fruits, and neglect below-ground roots. Yet all acknowledge roots are important for anchorage, water and nutrient uptake, and presumably components of yield. This paper investigates the relationship between domestication, and the root systems of landraces, and the parents of early, mid- and late green-revolution bread wheat cultivars. It compares the root system of bread wheat and ‘Veery’-type wheat containing the 1RS translocation from rye. Methods Wheat germplasm was grown in large pots in sand culture in replicated experiments. This allowed roots to be washed free to study root characters. Key Results The three bread wheat parents of early green-revolution wheats have root biomass less than two-thirds the mean of some landrace wheats. Crossing early green-revolution wheat to an F2 of ‘Norin 10’ and ‘Brevor’, further reduced root biomass in mid-generation semi-dwarf and dwarf wheats. Later-generation semi-dwarf wheats show genetic variation for root biomass, but some exhibit further reduction in root size. This is so for some California and UK wheats. The wheat–rye translocation in ‘Kavkaz’ for the short arm of chromosome 1 (1RS) increased root biomass and branching in cultivars that contained it. Conclusions Root size of modern cultivars is small compared with that of landraces. Their root system may be too small for optimum uptake of water and nutrients and maximum grain yield. Optimum root size for grain yield has not been investigated in wheat or most crop plants. Use of 1RS and similar alien translocations may increase root biomass and grain yield significantly in irrigated and rain-fed conditions. Root characters may be integrated into components of yield analysis in wheat. Plant breeders may need to select directly for root characters.
Prediction Signatures in the Brain: Semantic Pre-Activation during Language Comprehension

There is broad agreement that context-based predictions facilitate lexical-semantic processing. A robust index of semantic prediction during language comprehension is an evoked response, known as the N400, whose amplitude is modulated as a function of semantic context. However, the underlying neural mechanisms that utilize relations of the prior context and the embedded word within it are largely unknown. We measured magnetoencephalography (MEG) data while participants were listening to simple German sentences in which the verbs were either highly predictive for the occurrence of a particular noun (i.e., provided context) or not. The identical set of nouns was presented in both conditions. Hence, differences for the evoked responses of the nouns can only be due to differences in the earlier context. We observed a reduction of the N400 response for highly predicted nouns. Interestingly, the opposite pattern was observed for the preceding verbs: highly predictive (that is more informative) verbs yielded stronger neural magnitude compared to less predictive verbs. A negative correlation between the N400 effect of the verb and that of the noun was found in a distributed brain network, indicating an integral relation between the predictive power of the verb and the processing of the subsequent noun. This network consisted of left hemispheric superior and middle temporal areas and a subcortical area; the parahippocampus. Enhanced activity for highly predictive relative to less predictive verbs, likely reflects establishing semantic features associated with the expected nouns, that is a pre-activation of the expected nouns.
Novel approach for selecting the best predictor for identifying the binding sites in DNA binding proteins

Protein–DNA complexes play vital roles in many cellular processes by the interactions of amino acids with DNA. Several computational methods have been developed for predicting the interacting residues in DNA-binding proteins using sequence and/or structural information. These methods showed different levels of accuracies, which may depend on the choice of data sets used in training, the feature sets selected for developing a predictive model, the ability of the models to capture information useful for prediction or a combination of these factors. In many cases, different methods are likely to produce similar results, whereas in others, the predictors may return contradictory predictions. In this situation, a priori estimates of prediction performance applicable to the system being investigated would be helpful for biologists to choose the best method for designing their experiments. In this work, we have constructed unbiased, stringent and diverse data sets for DNA-binding proteins based on various biologically relevant considerations: (i) seven structural classes, (ii) 86 folds, (iii) 106 superfamilies, (iv) 194 families, (v) 15 binding motifs, (vi) single/double-stranded DNA, (vii) DNA conformation (A, B, Z, etc.), (viii) three functions and (ix) disordered regions. These data sets were culled as non-redundant with sequence identities of 25 and 40% and used to evaluate the performance of 11 different methods in which online services or standalone programs are available. We observed that the best performing methods for each of the data sets showed significant biases toward the data sets selected for their benchmark. Our analysis revealed important data set features, which could be used to estimate these context-specific biases and hence suggest the best method to be used for a given problem. We have developed a web server, which considers these features on demand and displays the best method that the investigator should use. The web server is freely available at http://www.biotech. iitm.ac. in/DNA-protein/. Further, we have grouped the methods based on their complexity and analyzed the performance. The information gained in this work could be effectively used to select the best method for designing experiments.
Handwritten Brazilian month recognition: an analysis of two NN architectures and a rejection mechanism

This paper evaluates the use of the conventional architecture feedforward MLP (multiple layer perceptron) and class-modular for the handwriting recognition (HWR) and it also compares the results obtained with previous works in terms of recognition rate. This work presents a feature set in full detail to work with HWR. The experiments showed that the class-modular architecture is better than conventional architecture. The obtained average recognition rates were 77.08% using the conventional architecture and 81.75% using the class-modular. This paper also describes a performance study in which a rejection mechanism with multiple thresholds is evaluated for both conventional and class-modular architectures. The multiple thresholds idea is based on the use of N class-related reject thresholds (CRTs). The results indicate that this rejection mechanism can be used appropriately in both architectures. The experimental results are 86.38% and 91.52% using a handwritten months word database.
Marginal adaptation of different monolithic zirconia crowns with horizontal and vertical finish lines: A comparative in vitro study

Background. This study evaluated the influence of different tooth preparation techniques and zirconia materials on marginal adaptation. Methods. Forty-eight healthy human maxillary first premolars were divided into two primary groups based on preparation design: group A (chamfer) and group B (vertical). Within each main group, there were three subgroups, comprising eight teeth each, distinguished by the type of zirconia material employed (Zircad LT, MT, and Prime by Ivoclar Vivadent). All the samples were prepared by the same operator using a dental surveyor. Intraoral scanning was performed on the prepared teeth. SironaInLab CAD 20.0 software was used to design crowns, which were subsequently generated using a 5-axis milling machine. The crowns were cemented to their respective teeth with self-adhesive resin cement. Marginal gap measurements were taken in micrometers (μm) before and after cementation at 16 sites per sample using a digital microscope at×230 magnification. The collected data were evaluated using statistical analysis using the independent t-test, paired t-test, and ANOVA at an 0.05 significance level. Results. The vertical preparation group exhibited the smallest marginal gap, while the chamfer group displayed the largest. This disparity was statistically significant (P<0.05) for pre- and post-cementation measurements across all materials. There were no significant differences between the different monolithic zirconia crowns. Conclusion. The vertical preparation design illustrated significantly better marginal adaptation than the chamfer preparation design. Comparisons between materials showed comparable marginal gaps. The mean values of the marginal gaps in all groups increased significantly after cementation.
Decoupled Freshwater Transport and Meridional Overturning in the South Atlantic

Freshwater transports (Fov) by the Atlantic meridional overturning circulation (AMOC) are sensitive to salinity distributions and may determine AMOC stability. However, climate models show large salinity biases, distorting the relation between Fov and the AMOC. Using free‐running models and ocean reanalyses with realistic salinities but quite different AMOCs, we show that the fresh Antarctic Intermediate Water layer eliminates salinity differences across the AMOC branches at ~1,200 m, ∆S1200m, which decouples Fov from the AMOC south of ~10°N. As the Antarctic Intermediate Water disappears north of ~10°N, a large ∆S1200m allows the AMOC to drive substantial southward Fov in the North Atlantic. In the South Atlantic the 0–300 m zonal salinity contrasts control the gyre freshwater transports Fgyre, which also determine the total freshwater transports. This decoupling makes the southern Fov unlikely to play any role in AMOC stability, leaving indirect Fgyre feedbacks or Fov in the north, as more relevant factors.
Profiling of Amatoxins and Phallotoxins in the Genus Lepiota by Liquid Chromatography Combined with UV Absorbance and Mass Spectrometry

Species in the mushroom genus Lepiota can cause fatal mushroom poisonings due to their content of amatoxins such as α-amanitin. Previous studies of the toxin composition of poisonous Lepiota species relied on analytical methods of low sensitivity or resolution. Using liquid chromatography coupled to UV absorbance and mass spectrometry, we analyzed the spectrum of peptide toxins present in six Italian species of Lepiota, including multiple samples of three of them collected in different locations. Field taxonomic identifications were confirmed by sequencing of the internal transcribed spacer (ITS) regions. For comparison, we also analyzed specimens of Amanita phalloides from Italy and California, a specimen of A. virosa from Italy, and a laboratory-grown sample of Galerina marginata. α-Amanitin, β-amanitin, amanin, and amaninamide were detected in all samples of L. brunneoincarnata, and α-amanitin and γ-amanitin were detected in all samples of L. josserandii. Phallotoxins were not detected in either species. No amatoxins or phallotoxins were detected in L. clypeolaria, L. cristata, L. echinacea, or L. magnispora. The Italian and California isolates of A. phalloides had similar profiles of amatoxins and phallotoxins, although the California isolate contained more β-amanitin relative to α-amanitin. Amaninamide was detected only in A. virosa.
Extraction and Isolation of Hemicelluloses from Agricultural and Forestry Waste Streams

New methods and products must be developed to reduce our dependency on fossil fuels, to reduce global warming. In this work, methods of extracting and isolating hemi¬celluloses from agricultural and forestry waste streams have been studied. Hemi¬celluloses are biodegradable polymers that can replace fossil-fuel-based products for use in various applications, such as barrier films and hydrogels. Arabinoxylan was extracted from barley husks, wheat straw and barley straw, by steam pretreatment, and then further concentrated and purified by conventional filtration, microfiltration and ultrafiltration. Xylan yields of 25 to 50 wt%, based on the content in the raw material, were achieved in the steam pretreatment stage by optimizing the operating conditions. The pH should be kept above 8 and a severity factor of about 4 used to obtain a high yield of arabinoxylan with large molar mass. The extraction of hemicelluloses from agricultural residues can be combined with ethanol production, which could be of great interest in future biorefineries. Galactoglucomannans were extracted from process water from a thermo¬mechanical pulp mill. These hemicelluloses were isolated by common filtration combined with membrane filtration. Drum filtration and microfiltration were used to remove solids and ultrafiltration to concentrate and purify the hemicelluloses. A hemicellulose concentration above 60 g/l was achieved with a purity of about 80%. The process developed is very promising, indicating that hemicelluloses could be produced at a cost of roughly €600 per tonne, although further work is required to prove the concept on a larger scale. (Less)
Gender differences in fascicular lengthening during eccentric contractions: the role of the patella tendon stiffness

Elastic tendons have been suggested to attenuate fascicle lengthening during eccentric contractions; however, there is no in vivo evidence to support this hypothesis. Therefore, the aim of this study was to determine whether patella tendon stiffness modulates vastus lateralis (VL) fascicle lengthening during eccentric contractions in males and females.