Miracle-dz/UniFAR-FTUs huggingface dataset:
Mechanism of Rectification in Inward-rectifier K+ Channels

Rectification in inward-rectifier K+ channels is caused by the binding of intracellular cations to their inner pore. The extreme sharpness of this rectification reflects strong voltage dependence (apparent valence is ∼5) of channel block by long polyamines. To understand the mechanism by which polyamines cause rectification, we examined IRK1 (Kir2.1) block by a series of bis-alkyl-amines (bis-amines) and mono-alkyl-amines (mono-amines) of varying length. The apparent affinity of channel block by both types of alkylamines increases with chain length. Mutation D172N in the second transmembrane segment reduces the channel's affinity significantly for long bis-amines, but only slightly for short ones (or for mono-amines of any length), whereas a double COOH-terminal mutation (E224G and E299S) moderately reduces the affinity for all bis-amines. The apparent valence of channel block increases from ∼2 for short amines to saturate at ∼5 for long bis-amines or at ∼4 for long mono-amines. On the basis of these and other observations, we propose that to block the channel pore one amine group in all alkylamines tested binds near the same internal locus formed by the COOH terminus, while the other amine group of bis-amines, or the alkyl tail of mono-amines, “crawls” toward residue D172 and “pushes” up to 4 or 5 K+ ions outwardly across the narrow K+ selectivity filter. The strong voltage dependence of channel block therefore reflects the movement of charges carried across the transmembrane electrical field primarily by K+ ions, not by the amine molecule itself, as K+ ions and the amine blocker displace each other during block and unblock of the pore. This simple displacement model readily accounts for the classical observation that, at a given concentration of intracellular K+, rectification is apparently related to the difference between the membrane potential and the equilibrium potential for K+ ions rather than to the membrane potential itself.
Evaluating voice speech quality in 802.11b networks with VPN/IPSec

In this paper we present an experimental analysis of voice traffic over 802.11b networks when protected by IPSecurity mechanisms. The main concern is to measure the impact on speech quality due these mechanisms by means of our corrected ITU-T E-Model implementation, showing how the associated security overhead decreases speech quality and channel capacity.
Rhein, a Natural Anthraquinone Derivative, Attenuates the Activation of Pancreatic Stellate Cells and Ameliorates Pancreatic Fibrosis in Mice with Experimental Chronic Pancreatitis

Pancreatic fibrosis, a prominent histopathological feature of chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma, is essentially a dynamic process that leads to irreversible scarring of parenchymal tissues of the pancreas. Though the exact mechanisms of its initiation and development are poorly understood, recent studies suggested that the activation of pancreatic stellate cells (PSCs) plays a critical role in eliciting such active course of fibrogenesis. Anthraquinone compounds possess anti-inflammatory bioactivities whereas its natural derivative rhein has been shown to effectively reduce tissue edema and free-radical production in rat models of inflammatory conditions. Apart from its anti-inflammatory properties, rhein actually exerts strong anti-fibrotic effects in our current in-vivo and in-vitro experiments. In the mouse model of cerulein-induced CP, prolonged administration of rhein at 50 mg/kg/day significantly decreased immunoreactivities of the principal fibrotic activators alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta (TGF-β) on pancreatic sections implicating the activation of PSCs, which is the central tread to fibrogenesis, was attenuated. Consequently, the overwhelmed deposition of extracellular matrix proteins fibronectin 1 (FN1) and type I collagen (COL I-α1) in exocrine parenchyma was found accordingly reduced. In addition, the expression levels of sonic hedgehog (SHH), which plays important roles in molecular modulation of various fibrotic processes, and its immediate effector GLI1 in pancreatic tissues were positively correlated to the degree of cerulein-induced fibrosis. Such up-regulation of SHH signaling was restrained in rhein-treated CP mice. In cultured PSCs, we demonstrated that the expression levels of TGF-β-stimulated fibrogenic markers including α-SMA, FN1 and COL I-α1 as well as SHH were all notably suppressed by the application of rhein at 10 μM. The present study firstly reported that rhein attenuates PSC activation and suppresses SHH/GLI1 signaling in pancreatic fibrosis. With strong anti-fibrotic effects provided, rhein can be a potential remedy for fibrotic and/or PSC-related pathologies in the pancreas.
Peptidases Compartmentalized to the Ascaris suum Intestinal Lumen and Apical Intestinal Membrane

The nematode intestine is a tissue of interest for developing new methods of therapy and control of parasitic nematodes. However, biological details of intestinal cell functions remain obscure, as do the proteins and molecular functions located on the apical intestinal membrane (AIM), and within the intestinal lumen (IL) of nematodes. Accordingly, methods were developed to gain a comprehensive identification of peptidases that function in the intestinal tract of adult female Ascaris suum. Peptidase activity was detected in multiple fractions of the A. suum intestine under pH conditions ranging from 5.0 to 8.0. Peptidase class inhibitors were used to characterize these activities. The fractions included whole lysates, membrane enriched fractions, and physiological- and 4 molar urea-perfusates of the intestinal lumen. Concanavalin A (ConA) was confirmed to bind to the AIM, and intestinal proteins affinity isolated on ConA-beads were compared to proteins from membrane and perfusate fractions by mass spectrometry. Twenty-nine predicted peptidases were identified including aspartic, cysteine, and serine peptidases, and an unexpectedly high number (16) of metallopeptidases. Many of these proteins co-localized to multiple fractions, providing independent support for localization to specific intestinal compartments, including the IL and AIM. This unique perfusion model produced the most comprehensive view of likely digestive peptidases that function in these intestinal compartments of A. suum, or any nematode. This model offers a means to directly determine functions of these proteins in the A. suum intestine and, more generally, deduce the wide array functions that exist in these cellular compartments of the nematode intestine.
Migration, Integration, Survival, and Differentiation of Stem Cell-Derived Neural Progenitors in the Retina in a Pharmacological Model of Retinal Degeneration

Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks old, were submitted to intraperitoneal injection of sodium iodate (2% NaIO3, 50 mg/kg). After 72 hours, 2 μL of solution with mNPC were injected intravitreally (100.000 cells/μL). After 7 days, their eyes were dissected and cryoprotected in 30% sucrose in PB for at least 24 hours at 4°C. The material was analyzed by immunohistochemistry and the following primary antibodies evaluation. Results. The results showed that the grafted cells integrated and survived in the adult mice within the sinner retinal tissue for at least 7 days. Immunohistochemical analysis revealed mature neuronal pattern in some regions. The mNPC population in the transplants was tightly surrounded by neuroretinal cells, suggesting their active role in neuron survival. Notably, the appearance of GFP-positive mNPC was not the result of fusion between donor cells and endogenous neuroretinal cells. Conclusions. Migration, survival, and differentiation of mNPCs were observed after 7 days following a single application with neurosphere method. The results may be clinically relevant for future stem cell therapy to restore retinal degeneration.
Efficient Spatio-Temporal Local Binary Patterns for Spontaneous Facial Micro-Expression Recognition

Micro-expression recognition is still in the preliminary stage, owing much to the numerous difficulties faced in the development of datasets. Since micro-expression is an important affective clue for clinical diagnosis and deceit analysis, much effort has gone into the creation of these datasets for research purposes. There are currently two publicly available spontaneous micro-expression datasets—SMIC and CASME II, both with baseline results released using the widely used dynamic texture descriptor LBP-TOP for feature extraction. Although LBP-TOP is popular and widely used, it is still not compact enough. In this paper, we draw further inspiration from the concept of LBP-TOP that considers three orthogonal planes by proposing two efficient approaches for feature extraction. The compact robust form described by the proposed LBP-Six Intersection Points (SIP) and a super-compact LBP-Three Mean Orthogonal Planes (MOP) not only preserves the essential patterns, but also reduces the redundancy that affects the discriminality of the encoded features. Through a comprehensive set of experiments, we demonstrate the strengths of our approaches in terms of recognition accuracy and efficiency.
Construal level and free will beliefs shape perceptions of actors' proximal and distal intent

Two components of lay observers' calculus of moral judgment are proximal intent (the actor's mind is focused on performing the action) and distal intent (the actor's mind is focused on the broader goal). What causes observers to prioritize one form of intent over the other? The authors observed whether construal level (Studies 1–2) and beliefs about free will (Studies 3–4) would influence participants' sensitivity to the actor's proximal vs. distal intent. In four studies, participants read scenarios in which the actor's proximal and distal intent were independently manipulated. In Study 1, when only distal intent was present in the actor's mind, participants rated the psychologically distant actor more responsible than the psychologically near actor. In Study 2, when only distal intent was in the actor's mind, participants with a chronic high level of action identification rated the actor more responsible than did those with a low level of action identification. In both studies, when only proximal intent was in the actor's mind, construal level did not predict judgments of responsibility. In Study 3, when only proximal intent was present in the actor's mind, the more participants believed in free will, the more they rated the actor responsible. When only distal intent was in the actor's mind, free will belief did not influence ratings of responsibility. In Study 4, the same pattern emerged when free will/determinism beliefs were manipulated and the actor performed a positive (life-saving) act. The authors discuss how these results shed new light on the literatures on moral reasoning and psycho-legal theory.
Maintenance therapy with budesonide and formoterol in chronicobstructive pulmonary disease

Lung function in chronic obstructive pulmonary disease (COPD) can be improved acutely by oral corticosteroids and bronchodilators. Whether clinical improvement can be maintained by subsequent inhaled therapy is unknown. COPD patients (n=1,022, mean prebronchodilator forced expiratory volume in one second (FEV1) 36% predicted) initially received formoterol (9 µg b.i.d.) and oral prednisolone (30 mg o.d.) for 2 weeks. After this time, patients were randomised to b.i. d. inhaled budesonide/formoterol 320/9 µg, budesonide 400 µg, formoterol 9 µg or placebo for 12 months. Postmedication FEV1 improved by 0.21 L and health-related quality of life using the St George's Respiratory Questionnaire (SGRQ) by 4.5 units after run-in. Fewer patients receiving budesonide/formoterol withdrew from the study than those receiving budesonide, formoterol or placebo. Budesonide/formoterol patients had a prolonged time to first exacerbation (254 versus 96 days) and maintained higher FEV1 (99% versus 87% of baseline), both primary variables versus placebo. They had fewer exacerbations (1.38 versus 1.80 exacerbations per patient per year), had higher prebronchodilator peak expiratory flow, and showed clinically relevant improvements in SGRQ versus placebo (−7.5 units). Budesonide/formoterol was more effective than either monocomponent in both primary variables. Budesonide/formoterol in a single inhaler (Symbicort®) maintains the benefit of treatment optimisation, stabilising lung function and delaying exacerbations more effectively than either component drug alone or placebo.
Baryon Census in Hydrodynamical Simulations of Galaxy Clusters

We carry out an analysis of a set of cosmological SPH hydrodynamical simulations of galaxy clusters and groups aimed at studying the total baryon budget in clusters, and how this budget is shared between the hot diffuse component and the stellar component. Using the TreePM+SPHGADGET-3 code, we carried out one set of non‐radiative simulations, and two sets of simulations including radiative cooling, star form ation and feedback from supernovae (SN), one of which also accounting for the effect of feedback from active galactic nuclei (AGN). The analysis is carried out with the twofold aim of studying the implication of stellar and hot gas content on the relative role played by SN and AGN feedback, and to calibrate the cluster baryon fraction and its evolution as a cosmological tool. With respect to previous similar analysis, the simulations used in this study provid e us with a sufficient statistics of massive objects and including an efficient AGN feedback. We fi nd that both radiative simulation sets predict a trend of stellar mass fraction with clu ster mass that tends to be weaker than the observed one. However this tension depends on the particular set of observational data considered. Including the effect of AGN feedback alleviates this tension on the stellar mass and predicts values of the hot gas mass fraction and total baryon fraction to be in closer agreement with observational results. We further compute the ratio between the cluster baryon content and the cosmic baryon fraction, Yb, as a function of cluster-centric radius and redshift. At R500 we find for massive clusters with M500 > 2×10 14 h −1 M⊙ that Yb is nearly independent of the physical processes included and characterized by a negligible redshift evolution: Yb,500 = 0.85 ± 0.03 with the error accounting for the intrinsic r.m.s. scatter w ithin the set of simulated clusters. At smaller radii, R2500, the typical value of Yb slightly decreases, by an amount that depends on the physics included in the simulations, while its scatter increases by about a factor of two. These results have interesting implications for the cosmological applications of the baryon fraction in clusters.
DTRACK: A System to Predict and Track Internet Path Changes

In this paper, we implement and evaluate a system that predicts and tracks Internet path changes to maintain an up-to-date network topology. Based on empirical observations, we claim that monitors can enhance probing according to the likelihood of path changes. We design a simple predictor of path changes and show that it can be used to enhance probe targeting. Our path tracking system, called DTRACK, focuses probes on unstable paths and spreads probes over time to minimize the chances of missing path changes. Our evaluations of DTRACK with trace-driven simulations and with a prototype show that DTRACK can detect up to three times more path changes than traditional trace-route-based topology mapping techniques.
Ensemble Reforecasting: Improving Medium-Range Forecast Skill Using Retrospective Forecasts

Abstract The value of the model output statistics (MOS) approach to improving 6–10-day and week 2 probabilistic forecasts of surface temperature and precipitation is demonstrated. Retrospective 2-week ensemble “reforecasts” were computed using a version of the NCEP medium-range forecast model with physics operational during 1998. An NCEP–NCAR reanalysis initial condition and bred modes were used to initialize the 15-member ensemble. Probabilistic forecasts of precipitation and temperature were generated by a logistic regression technique with the ensemble mean (precipitation) or ensemble mean anomaly (temperature) as the only predictor. Forecasts were computed and evaluated during 23 winter seasons from 1979 to 2001. Evaluated over the 23 winters, these MOS-based probabilistic forecasts were skillful and highly reliable. When compared against operational NCEP forecasts for a subset of 100 days from the 2001–2002 winters, the MOS-based forecasts were comparatively much more skillful and reliable. For examp.
Comparison of Three Single-Phase PLL Algorithms for UPS Applications

In this paper, the performance assessment of three software single-phase phase-locked loop (PLL) algorithms is carried out by means of dynamic analysis and experimental results. Several line disturbances such as phase-angle jump, voltage sag, frequency step, and harmonics are generated by a DSP together with a D/A converter and applied to each PLL. The actual minus the estimated phase-angle values are displayed, providing a refined method for performance evaluation and comparison. Guidelines for parameters adjustments are also presented. In addition, practical implementation issues such as computational delay effects, ride-through, and computational load are addressed. The developed models proved to accurately represent the PLLs under real test conditions.
An ultracold and ultrafast electron source

We present measurements of the pulse length of ultracold electron bunches generated by near-threshold two-photon photoionisation of a laser-cooled gas. The pulse length has been measured using a resonant 3 GHz deflecting cavity in TM110 mode. We have measured the pulse length in three ionisation regimes. The first is direct two-photon photoionisation using only a 480 nm femtosecond laser pulse, which results in short (∼ 15 ps) but hot (∼ 10 K) electron bunches. The second regime is just-above-threshold femtosecond photoionisation employing the combination of a continuous-wave 780 nm excitation laser and a tunable 480 nm femtosecond ionisation laser which results in both ultracold (∼ 10 K) and ultrashort (∼ 25 ps) electron bunches. These pulses typically contain ∼ 10 electrons and have an root-meansquared normalised transverse beam emittance of 1.5 ± 0.1 nm·rad. The measured pulse lengths are limited by the energy spread associated with the longitudinal size of the ionisation volume, as expected. The third regime is just-below-threshold ionisation which produces Rydberg states which slowly ionise on microsecond time scales. 1The work described in this Chapter is published by J.G. H. Franssen, T.L. I. Frankort, E.J.D. Vredenbregt and O.J. Luiten in Struct. Dyn. 4, 044010 (2017).
Refraining from reporting crimes: accounts from young male crime victims with an immigrant background

ABSTRACT Interactions between the police and young people with an immigrant background are well researched internationally and are often discussed in the context of discrimination. Such interactions may explain, at least in part, why these young people do not report crimes to the police when they are the victims of crimes. This article reports accounts from young crime victims who have an immigrant background. The young men who were interviewed mainly portrayed their decisions not to notify the police in the context of masculinity discourse rather than using a discourse of discrimination, even though the interviewers asked specifically about whether discrimination or trust in the police influenced their decision. We argue that these young men’s accounts reflect their preferred self-presentations, since a dicriminination discourse may be tied to a victim identity that is incompatible with hegemonic masculinity.
Association between Urinary N-Acetyl-Beta-D-Glucosaminidase and Microalbuminuria in Diabetic Black Africans

Diabetes mellitus is the commonest cause of ESRD worldwide and third most common cause in Nigeria. Recent reports from Nigeria indicate the prevalence of diabetic nephropathy as an aetiology of ESRD is increasing necessitating early diagnosis of diabetic nephropathy. We measured the urinary excretion of N-acetyl-beta-D-glucosaminidase (NAG), NAG/creatinine ratio, urinary protein-creatinine ratio and calculated eGFR in 30 recently diagnosed nonhypertensive diabetics and 67 controls. The age and sex distribution, systolic blood pressure, serum and urinary creatinine were similar for both groups. There was higher urinary excretion of NAG (304 versus 184 μ mol/h/L, P < 0.001) and NAG/creatinine ratio (21.2 versus 15.7 μ mol/h/L/mmolCr, P < 0.001) in the diabetics than controls. There was a strong correlation between NAG/creatinine ratio and albumin/creatinine ratio (r = 0.74, P < 0.001). A multivariate linear regression model showed a significant linear relationship between NAG/creatinine ratio and albumin/creatinine ratio after adjusting for the effect of blood pressure, age, sex, and serum creatinine. The strong association found between albumin/creatinine ratio and NAG/creatinine ratio perhaps indicates the need for further investigation of the clinical utility of NAG/creatinine ratio as a screening tool for early nephropathy in African diabetics.
Small permutation classes

We establish a phase transition for permutation classes (downsets of permutations under the permutation containment order): there is an algebraic number κ, approximately 2.20557, for which there are only countably many permutation classes of growth rate (Stanley–Wilf limit) less than κ but uncountably many permutation classes of growth rate κ, answering a question of Klazar. We go on to completely characterize the possible sub‐κ growth rates of permutation classes, answering a question of Kaiser and Klazar. Central to our proofs are the concepts of generalized grid classes (introduced herein), partial well‐order, the substitution decomposition, and atomicity (a.k.a. the joint embedding property).
A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP

Long-term depression (LTD) at cerebellar parallel fiber (PF)-Purkinje cell synapses must be balanced by long-term potentiation (LTP) to prevent saturation and allow reversal of motor learning. The only previously analyzed form of cerebellar LTP is induced by 4–8 Hz PF stimulation and requires cAMP but not nitric oxide. It is a poor candidate to reverse LTD because it is presynaptically expressed whereas LTD is postsynaptic. We now characterize a new form of LTP induced by 1 Hz PF stimulation for at least 300 s. This LTP is postsynaptically expressed, enhanced by chelating postsynaptic Ca2+, and depends on nitric oxide but not cAMP or cGMP, making it a plausible anti-Hebbian counterpart to Hebbian LTD.