Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in Rabbit Febrile Responses to Muramyl Dipeptide (MDP) After Coupling to a Synthetic Carrier

Muramyl dipeptide (MDP) is a small molecular weight synthetic glycopeptide (< 500), which has been shown to be an immunoadjuvant, and to induce a biphasic febrile response in the rabbit—probably via the release of endogenous pyrogen—accompanied by a marked leukopenia. Macromolecularization by coupling to a synthetic carrier (MW = 60,000) potentiates the immunostim‐ulant properties of MDP but also its pyrogenicity. The present study demonstrates that such a conjugate induced the release of endogenous pyrogen in vivo and in vitro at lower dosage levels than free MDP. Further experiments showed that there existed several differences between free and conjugated MDP. Thus, after intravenous administration of the conjugate, the fever pattern was monophasic with a prompt defervescence and not accompanied by leukopenia at dosage levels inducing similar increase in body temperature. In addition, when fever was recorded after intracerebroventricular administration, the increase in sensitivity was much greater in the case of free MDP than of MDP‐A‐L.
Coping With Bullying in Schools

Part 1: the theoretical background definitions of bullying behaviour the incidence of bullying behaviour causes of bullying traits of bullies and the victims of bullying the longitudinal aspect of bullying behaviour. Part 2: bullies and victims in schools - author's findings. Part 3: teacher and pupil perception of bullying behaviour principles teachers pupil interviews. Part 4: profiles of some bullies and victims. Part 5: preventive and treatment approaches parents principals teachers the role of senior staff non-teaching staff.
Variation in Wood Quality in White Spruce (Picea Glauca (Moench) Voss). Part I. Defining the Juvenile–Mature Wood Transition Based on Tracheid Length

Estimations of transition age (TA) and juvenile wood proportion (JWP) are important for wood industries due to their impact on end-product quality. However, the relationships between analytical determination of TA based on tracheid length (TL) and recognized thresholds for adequate end products have not yet been established. In this study, we used three different statistical models to estimate TA in white spruce (Picea glauca (Moench) Voss) based on TL radial variation. We compared the results with technological maturity. A two-millimeter threshold, previously suggested for good paper tear strength, was used. Tracheid length increased from pith to bark and from breast height to upper height. Juvenile wood (JW) was conical with the three models. At breast height, TA ranged from 11 to 27 years and JWP ranged from 15.3% to 47.5% across the three models. The linear mixed model produced more conservative estimates than the maximum-quadratic-linear (M_Q_L) model. Both the linear mixed model and the M_Q_L model produced more conservative TA estimates than the piecewise model. TA estimates by the MIXED model, and to a lesser extent by the M_Q_L model, were equivalent to those for real mature wood, whereas TA estimates by the piecewise model were considerably lower, falling into the transition wood area.
Interaction of rat liver lysosomal membranes with actin

Membranes were prepared from lysosomes purified 80-fold by centrifugation in a discontinuous metrizamide gradient. When salt- washed membranes were combined with rabbit muscle actin, an increase in viscosity could be measured using a falling ball viscometer. The lysosomal membrane-actin interaction was actin- and membrane- concentration dependent and appeared to be optimal under presumed physiological conditions (2 mM MgCl2, 1 mM MgATP, neutral pH, and free calcium concentration less than 10(-8) M). The actin cross-linking activity of the membrane was optimal at pH 6.4. The interaction was maximal between 10(-7) and 10(-9) M free calcium ions and inhibited by approximately 50% at concentrations of calcium greater than 0.5 x 10(- 7) M. The actin-lysosomal membrane interaction was destroyed if the membranes were pretreated with Pronase, or if the membranes were purified in the absence of protease inhibitors. The interaction was not destroyed if membranes were washed with high salt or extracted with KCl and urea. In addition, a sedimentation assay for the actin-lysosomal membrane interaction was also performed to corroborate the viscometry data. The results suggest the existence of an integral lysosomal membrane actin-binding protein.
Effects of nitrogen addition on leaf decomposition of single-species and litter mixture in Pinus tabulaeformis forests

The litter decomposition process is closely correlated with nutrient cycling and the maintenance of soil fertility in the forest ecosystem. In particular, the intense environmental concern about atmospheric nitrogen (N) deposition requires a better understanding of its influence on the litter decomposition process. This study examines the responses of single-species litter and litter mixture decomposition processes to N addition in Chinese pine (Pinus tabulaeformis Carr.) ecosystems. Chinese pine litter, Mongolian oak (Quercus mongolica Fisch. ex Ledeb.) litter, and a pine–oak mixture were selected from a plantation and a natural forest of Chinese pine. Four N addition treatments, i.e., control (N0: 0 kg N ha−1·year−1), low-N (N1: 5 kg N ha−1·year−1), medium-N (N2: 10 kg N ha−1·year−1), and high-N (N3: 15 kg N ha−1·year−1), were applied starting May 2010. In the plantation, N addition significantly stimulated the decomposition of the Chinese pine litter. In the natural forest, N addition had variable effects on the decomposition of single-species litter and the litter mixture. A stimulatory effect of the high-N treatment on the Chinese pine litter decomposition could be attributed to a decrease in the substrate C:N ratio. However, an opposite effect was found for the Mongolian oak litter decomposition. The stimulating effect of N addition on the Chinese pine litter may offset the suppressive effect on the Mongolian oak litter, resulting in a neutral effect on the litter mixture. These results suggest that the different responses in decomposition of single-species litter and the litter mixture to N addition are mainly attributed to litter chemical composition. Further investigations are required to characterize the effect of long-term high-level N addition on the litter decomposition as N deposition is likely to increase rapidly in the region where this study was conducted.
International Perspectives on Adolescent and Young Adult Drinking

Alcohol consumption by adolescents and young adults varies greatly in different countries and cultures, in different population groups within a country, and over time. Analyses of per capita consumption in different countries provide some information on drinking patterns of young people in various countries. School-based surveys conducted in a variety of European countries and in the United States offer more specific insight into the drinking behavior of this age group. Such surveys have analyzed variables such as age of onset of drinking; lifetime frequency of drinking; drinking to intoxication; frequency, amount, and timing of current drinking; and drinking consequences. These studies have demonstrated that drinking patterns of young people in, for example, Scandinavian, Anglo-Saxon, and Mediterranean countries vary greatly. Further analyses have explored the influence of social norms and related factors as well as alcohol availability and pricing on alcohol consumption among adolescents and young adults. The generalizability of the findings is limited, however, by the fact that most studies have been conducted in the United States and Europe.
Chimeric Sex-Determining Chromosomal Regions and Dysregulation of Cell-Type Identity in a Sterile Zygosaccharomyces Allodiploid Yeast

Allodiploidization is a fundamental yet evolutionarily poorly characterized event, which impacts genome evolution and heredity, controlling organismal development and polyploid cell-types. In this study, we investigated the sex determination system in the allodiploid and sterile ATCC 42981 yeast, a member of the Zygosaccharomyces rouxii species complex, and used it to study how a chimeric mating-type gene repertoire contributes to hybrid reproductive isolation. We found that ATCC 42981 has 7 MAT-like (MTL) loci, 3 of which encode α-idiomorph and 4 encode a-idiomorph. Two phylogenetically divergent MAT expression loci were identified on different chromosomes, accounting for a hybrid a/α genotype. Furthermore, extra a-idimorph-encoding loci (termed MTLa copies 1 to 3) were recognized, which shared the same MATa1 ORFs but diverged for MATa2 genes. Each MAT expression locus was linked to a HML silent cassette, while the corresponding HMR loci were located on another chromosome. Two putative parental sex chromosome pairs contributed to this unusual genomic architecture: one came from an as-yet-undescribed taxon, which has the NCYC 3042 strain as a unique representative, while the other did not match any MAT-HML and HMR organizations previously described in Z. rouxii species. This chimeric rearrangement produces two copies of the HO gene, which encode for putatively functional endonucleases essential for mating-type switching. Although both a and α coding sequences, which are required to obtain a functional cell-type a1-α2 regulator, were present in the allodiploid ATCC 42981 genome, the transcriptional circuit, which regulates entry into meiosis in response to meiosis-inducing salt stress, appeared to be turned off. Furthermore, haploid and α-specific genes, such as MATα1 and HO, were observed to be actively transcribed and up-regulated under hypersaline stress. Overall, these evidences demonstrate that ATCC 42981 is unable to repress haploid α-specific genes and to activate meiosis in response to stress. We argue that sequence divergence within the chimeric a1-α2 heterodimer could be involved in the generation of negative epistasis, contributing to the allodiploid sterility and the dysregulation of cell identity.
Finite time bounds for sampling based fitted value iteration

In this paper we consider sampling based fitted value iteration for discounted, large (possibly infinite) state space, finite action Markovian Decision Problems where only a generative model of the transition probabilities and rewards is available. At each step the image of the current estimate of the optimal value function under a Monte-Carlo approximation to the Bellman-operator is projected onto some function space. PAC-style bounds on the weighted Lp-norm approximation error are obtained as a function of the covering number and the approximation power of the function space, the iteration number and the sample size.
A Mouse Model for Conditional Secretion of Specific Single-Chain Antibodies Provides Genetic Evidence for Regulation of Cortical Plasticity by a Non-cell Autonomous Homeoprotein Transcription Factor

During postnatal life the cerebral cortex passes through critical periods of plasticity allowing its physiological adaptation to the environment. In the visual cortex, critical period onset and closure are influenced by the non-cell autonomous activity of the Otx2 homeoprotein transcription factor, which regulates the maturation of parvalbumin-expressing inhibitory interneurons (PV cells). In adult mice, the maintenance of a non-plastic adult state requires continuous Otx2 import by PV cells. An important source of extra-cortical Otx2 is the choroid plexus, which secretes Otx2 into the cerebrospinal fluid. Otx2 secretion and internalization requires two small peptidic domains that are part of the DNA-binding domain. Thus, mutating these “transfer” sequences also modifies cell autonomous transcription, precluding this approach to obtain a cell autonomous-only mouse. Here, we develop a mouse model with inducible secretion of an anti-Otx2 single-chain antibody to trap Otx2 in the extracellular milieu. Postnatal secretion of this single-chain antibody by PV cells delays PV maturation and reduces plasticity gene expression. Induced adult expression of this single-chain antibody in cerebrospinal fluid decreases Otx2 internalization by PV cells, strongly induces plasticity gene expression and reopens physiological plasticity. We provide the first mammalian genetic evidence for a signaling mechanism involving intercellular transfer of a homeoprotein transcription factor. Our single-chain antibody mouse model is a valid strategy for extracellular neutralization that could be applied to other homeoproteins and signaling molecules within and beyond the nervous system.
The missing, the short, and the long: Levodopa responses and dopamine actions

We attempt to correlate the clinical pharmacology of dopamine replacement therapy (DRT) in Parkinson Disease with known features of striatal dopamine actions. Despite its obvious impact, DRT does not normalize motor function, likely due to disrupted phasic dopaminergic signaling. The DRT Short Duration Response is likely a permissive-paracrine effect, possibly resulting from dopaminergic support of corticostriate synaptic plasticity. The DRT Long Duration Response may result from mimicry of tonic dopamine signaling regulation of movement vigor. Our understanding of dopamine actions does not explain important aspects of DRT clinical pharmacology. Reducing these knowledge gaps provides opportunities to improve understanding of dopamine actions and symptomatic treatment of Parkinson disease. This article is protected by copyright. All rights reserved.
A Comparison between Sludge Ash and Fly Ash on the Improvement in Soft Soil

Abstract In this study, the strength of soft cohesive subgrade soil was improved by applying sewage sludge ash as a soil stabilizer. Test results obtained were compared with earlier tests conducted on soil samples treated with fly ash. Five different proportions of sludge ash and fly ash were mixed with soft cohesive soil, and tests such as pH value, compaction, California bearing ratio, unconfined compressive strength (UCS), and triaxial compression were performed to understand soil strength improvement because of the addition of both ashes. Results indicate that pH values increase with extending curing age for soil with sludge ash added. The UCS of sludge ash/soil were 1.4–2 times better than untreated soil. However, compressive strength of sludge ash/soil was 20–30 kPa less than fly ash/soil. The bearing capacities for both fly ash/soil and sludge ash/soil were five to six times and four times, respectively, higher than the original capacity. Moreover, the cohesive parameter of shear strength rose with increased amounts of either ash added. Friction angle, however, decreased with increased amounts of either ash. Consequently, results show that sewage sludge ash can potentially replace fly ash in the improvement of the soft cohesive soil.
Early Career Earnings of African American Students: The Impact of Attendance at Historically Black versus White Colleges and Universities

Abstract:Through nine-year longitudinal data and two-level hierarchical linear models, this study found that African American students have a similar prospect for early career earnings whether they attended an historically Black college and university (HBCU) or an historically White college and university (HWCU). Regardless of the type of institution they attended, women respondents earned significantly less than male counterparts. Peer orientation of outside work during college was a negative predictor for career earnings. The analysis of supplemental data suggests that a higher percentage of African American students attending HBCUs reported strong growth in job-related skills and preparation for graduate/professional schools than their counterparts at HWCUs. According to the variability analysis, African Americans' early career earnings are most likely a function of individual assets or characteristics rather than of institutional characteristics.
Influence of glucagon-like peptide 1 on fasting glycemia in type 2 diabetic patients treated with insulin after sulfonylurea secondary failure.

OBJECTIVE Glucagon-like peptide 1 (GLP-1) has glucose-dependent insulinotropic and glucagonostatic actions in type 2 diabetic patients on diet and on oral agents. It is not known, however, whether after secondary sulfonylurea failure, GLP-1 is still effective. RESEARCH DESIGN AND METHODS Therefore, 10 type 2 diabetic patients (6 women, 4 men; age 65+/-10 years, BMI 30.4+/-5.1 kg/m2, HbA1c 8.2+/-1.5%, 6+/-3 [2-13] years after starting insulin treatment) were examined in the fasting state after discontinuing NPH insulin on the evening before the two study days. GLP-1 (1.2 pmol x kg(-1) x min(-1) or placebo (NaCl with 1% human serum albumin) were infused over 6 h. Plasma glucose (glucose oxidase) insulin (IMx), and C-peptide (enzyme-linked immunosorbent assay) were measured. Statistical analysis was performed using repeated measures analysis of variance. RESULTS Fasting plasma glucose was 9.4+/-0.5 mmol/l and was reduced by GLP-1 to 5.3+/-0.3 (3.9-7.3) mmol/l (placebo: 8.2+/-0.7 mmol/l; P < 0.0001). GLP-1 transiently increased insulin (from 115+/-31 to 222+/-64 pmol/l at 150 min; P < 0.0001) and C-peptide (from 1.00+/-0.12 to 1.90+/-0.23 nmol/l at 120 min; P < 0.0001) with no effect of placebo. Glucagon and free fatty acids were lowered transiently. After normalization of plasma glucose, insulin and C-peptide concentrations became lower again during the ongoing administration of exogenous GLP-1, and no hypoglycemia occurred. CONCLUSIONS It is concluded that exogenous GLP-1 effectively lowers plasma glucose concentrations in advanced type 2 diabetes long after sulfonylurea secondary failure. These findings may broaden the applicability of GLP-1-derived drugs as a new treatment to nearly all type 2 diabetic patients.
Comparisons and computation of well-founded semantics for disjunctive logic programs

Much work has been done on extending the well-founded semantics to general disjunctive logic programs and various approaches have been proposed. However, these semantics are different from each other and no consensus is reached about which semantics is the most intended. In this article, we look at disjunctive well-founded reasoning from different angles. We show that there is an intuitive form of the well-founded reasoning in disjunctive logic programming which can be characterized by slightly modifying some existing approaches to defining disjunctive well-founded semantics, including program transformations, argumentation, unfounded sets (and resolution-like procedure). By employing the techniques developed by Brass and Dix in their transformation-based approach, we also provide a bottom-up procedure for this semantics. The significance of our work is not only in clarifying the relationship among different approaches, but also shed some light on what is an intended well-founded semantics for disjunctive logic programs.
Synthetic Lethal Targeting of PTEN-Deficient Cancer Cells Using Selective Disruption of Polynucleotide Kinase/Phosphatase

A recent screen of 6,961 siRNAs to discover possible synthetic lethal partners of the DNA repair protein polynucleotide kinase/phosphatase (PNKP) led to the identification of the potent tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN). Here, we have confirmed the PNKP/PTEN synthetic lethal partnership in a variety of different cell lines including the PC3 prostate cancer cell line, which is naturally deficient in PTEN. We provide evidence that codepletion of PTEN and PNKP induces apoptosis. In HCT116 colon cancer cells, the loss of PTEN is accompanied by an increased background level of DNA double-strand breaks, which accumulate in the presence of an inhibitor of PNKP DNA 3′-phosphatase activity. Complementation of PC3 cells with several well-characterized mutated PTEN cDNAs indicated that the critical function of PTEN required to prevent toxicity induced by an inhibitor of PNKP is most likely associated with its cytoplasmic lipid phosphatase activity. Finally, we show that modest inhibition of PNKP in a PTEN knockout background enhances cellular radiosensitivity, suggesting that such a “synthetic sickness” approach involving the combination of PNKP inhibition with radiotherapy may be applicable to PTEN-deficient tumors. Mol Cancer Ther; 12(10); 2135–44. ©2013 AACR.
New Method for Measuring Statistical Distributions of Partial Discharge Pulses

A new digital detection system is described for measuring pulsating partial discharges (PDs). The PD detection system can continuously record all PD pulses that occur over extended periods of time, with a minimum inter-pulse time separation of 6 μs and a vertical amplitude resolution of 12 bits. Earlier PD detection systems detected PD pulse amplitude and time using complex custom-designed hardware while the present system continuously records the complete electrical waveform that carries the PD pulses using a commercial data acquisition board and extracts, in real time, the time and amplitude information of all PD pulses in software. The current approach considerably reduces the development and maintenance cost of the PD detection system, significantly increases the system portability, and may prove to be a crucial step for transferring the digital PD detection and analysis technology developed in laboratories to industry. The features of the new system are illustrated by the study of dc-excited PD pulses occurring in a point-to-plane gap in air. A new surface-mediated burst mode of PDs is discovered in which a PD pulse has a certain probability to induce another pulse. The probability is determined for several gap voltages and is found to vary strongly with the applied voltage.
Prenatal ascertainment of OEIS complex/cloacal exstrophy—15 new cases and literature review

Omphalocele‐exstrophy of the bladder‐imperforate anus‐spinal defects (OEIS) complex or cloacal exstrophy (EC), describes a rare grouping of more commonly occurring component malformations [Carey et al., 1978 ]. The etiology is unknown, but likely heterogeneous. While postnatal identification of its associated gastrointestinal, spinal, and genitourinary systems delineates the extent and natural history of OEIS complex, prenatal findings may provide additional information regarding early detection, possible causative factors, and outcome. The purposes of this study were to: (1) present the prenatal ascertainment of OEIS complex in this series of 15 cases identified through several different sources compared to the literature, and (2) discuss the relationship of these prenatal findings to possible abnormal developmental mechanisms causing OEIS complex. These 15 cases indicate that OEIS complex may be difficult to diagnose prenatally, and that the full extent of abnormalities may not be clear until postnatal exam. Confusion with limb‐body wall complex (two of our cases) and pentalogy of Cantrell (one of our cases) can occur. Anal/gastrointestinal malformations and genital ambiguity are under‐ascertained. Conversely, prenatal defects may resolve postnatally, yet may provide clues for pathogenetic mechanisms. For instance, the finding of nuchal thickening in our three cases (one reported) suggests vascular/hemodynamic compromise early in embryologic development, or intrathoracic compression leading to jugular lymphatic obstruction may play a role. The association of twinning and OEIS complex suggests they may occur as early as blastogenesis. Our three sets of discordant twins also suggest a non‐genetic etiology for OEIS complex of uteroplacental insufficiency. This study also indicates that OEIS complex may be more common than previously thought. © 2007 Wiley‐Liss, Inc.