Miracle-dz/UniFAR-FTUs huggingface dataset:
Just Say NO to Paxos Overhead: Replacing Consensus with Network Ordering

Distributed applications use replication, implemented by protocols like Paxos, to ensure data availability and transparently mask server failures. This paper presents a new approach to achieving replication in the data center without the performance cost of traditional methods. Our work carefully divides replication responsibility between the network and protocol layers. The network orders requests but does not ensure reliable delivery - using a new primitive we call ordered unreliable multicast (OUM). Implementing this primitive can be achieved with near-zero-cost in the data center. Our new replication protocol, Network-Ordered Paxos (NOPaxos), exploits network ordering to provide strongly consistent replication without coordination. The resulting system not only outperforms both latency - and throughput-optimized protocols on their respective metrics, but also yields throughput within 2% and latency within 16 ms of an unreplicated system - providing replication without the performance cost.
Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI

Bioluminescence imaging (BLI) has found significant use in evaluating long‐term cancer therapy in small animals. We have now tested the feasibility of using BLI to assess acute effects of the vascular disrupting agent combretastatin A4 phosphate (CA4P) on luciferase‐expressing MDA‐MB‐231 human breast tumor cells growing as xenografts in mice. Following administration of luciferin substrate, there is a rapid increase in light emission reaching a maximum after about 6 min, which gradually decreases over the following 20 min. The kinetics of light emission are highly reproducible;however, following i.p. administration of CA4P (120 mg/kg), the detected light emission was decreased between 50 and 90%, and time to maximum was significantly delayed. Twenty‐four hours later, there was some recovery of light emission following further administration of luciferin substrate. Comparison with dynamic contrast‐enhanced MRI based on the paramagnetic contrast agent Omniscan showed comparable changes in the tumors consistent with the previous literature. Histology also confirmed shutdown of tumor vascular perfusion. We believe this finding provides an important novel application for BLI that could have widespread application in screening novel therapeutics expected to cause acute vascular changes in tumors. —Zhao, D. , Richer, E. , Antich, P. P., Mason, R. P. Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI. FASEB J. 22, 2445–2451 (2008)
Vitamin B 12 biosynthesis gene diversity in the Ross Sea : the identification of a new group of putative polar B 12 biosynthesizersemi

Erin M. Bertrand,1,2,3* Mak A. Saito,2 Young Jae Jeon3 and Brett A. Neilan3,4 MIT/WHOI Joint Program in Chemical Oceanography, Woods Hole, MA 02543, USA. Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia. Australian Centre for Astrobiology, University of New South Wales, Sydney, New South Wales 2052, Australia.
Profiling of Volatile Compounds and Associated Gene Expression and Enzyme Activity during Fruit Development in Two Cucumber Cultivars

Changes in volatile content, as well as associated gene expression and enzyme activity in developing cucumber fruits were investigated in two Cucumis sativus L. lines (No. 26 and No. 14) that differ significantly in fruit flavor. Total volatile, six-carbon (C6) aldehyde, linolenic and linoleic acid content were higher during the early stages, whereas the nine-carbon (C9) aldehyde content was higher during the latter stages in both lines. Expression of C. sativus hydroperoxide lyase (CsHPL) mirrored 13-hydroperoxide lyase (13-HPL) enzyme activity in variety No. 26, whereas CsHPL expression was correlated with 9-hydroperoxide lyase (9-HPL) enzyme activity in cultivar No. 14. 13-HPL activity decreased significantly, while LOX (lipoxygenase) and 9-HPL activity increased along with fruit ripening in both lines, which accounted for the higher C6 and C9 aldehyde content at 0-6 day post anthesis (dpa) and 9-12 dpa, respectively. Volatile compounds from fruits at five developmental stages were analyzed by principal component analysis (PCA), and heatmaps of volatile content, gene expression and enzyme activity were constructed.
Optimism and quality of life in patients with heart failure

Abstract Objectives Health-related quality of life (HR-QoL) of patients with heart failure (HF) is low despite the aim of HF treatment to improve HR-QoL. To date, most studies have focused on medical and physical factors in relation to HR-QoL, few data are available on the role of emotional factors such as dispositional optimism. This study examines the prevalence of optimism and pessimism in HF patients and investigates how optimism and pessimism are associated with different patient characteristics and HR-QoL. Methods Dispositional optimism was assessed in 86 HF patients (mean age 70 ± 9 years, 28% female, mean left ventricular ejection fraction 33%) with the Revised Life Orientation Test (LOT-R). HR-QoL was assessed with the Minnesota Living with Heart Failure Questionnaire and the EuroQol. Results The (mean ± SD) total score on the LOT-R was 14.6 ± 2.9 (theoretical range 0–24) and the scores on the subscales optimism and pessimism were 8.1 ± 1.9 and 5.5 ± 2.5, respectively. Higher age was related to more optimism (r = 0.22, p < 0.05), and optimism was associated with higher generic HR-QoL (B = 0.04, p < 0.05). Significance of results The association found between optimism and generic HR-QoL of HF patients can lead to promising strategies to improve HF patients’ HR-QoL, particularly because the literature has indicated that optimism is a modifiable condition.
Reproductive Endocrinology and Musth Indicators in a Captive Asian Elephant (Elephas maximus)

Even in the best situations, the artificial social constructs of captivity alter natural elephant behavior and unfortunately create distress. Asian elephants are powerful and intelligent animals that require consideration for their well-being and prudent management. The males present particular difficulties due to a temporary state of heightened aggressive behavior unique to male elephants called “musth. ” When he is in this state, the danger the elephant poses to other animals and the people around him is considerable. In addition to antagonistic behavior, musth is also characterized by temporal gland secretion and urine dribbling. In previous studies, musth has been attributed to elevated testosterone levels. This study attempted to enhance the knowledge base concerning these phenomena by examining hormone concentrations (n = 357) in Onyx, a male Asian elephant housed at Dickerson Park Zoo, with intermittent access to females (n = 1–5) over a 12-year period. Behavior and signs of musth also were recorded daily by elephant department staff. Musth indicators (temporal gland secretion, aggression, urine dribbling) increased with musth but not prior to it. We confirmed that temporal gland secretion was a better indicator of behavioral musth than urine dribbling. Hormones concentrations increased as musth approached, and presumably initiated musth indicators, but variability was high. Therefore, these hormones cannot be used to predict the onset of musth in this individual. Rather, the free/total testosterone ratio was a good indication of the 60-day pre-musth period. In addition, testosterone production and musth indicators increased in intensity when a young bull at the zoo started entering musth.
Safety and efficacy of velaglucerase alfa in Gaucher disease type 1 patients previously treated with imiglucerase

Velaglucerase alfa is a glucocerebrosidase produced by gene activation technology in a human fibroblast cell line (HT‐1080), and it is indicated as an enzyme replacement therapy (ERT) for the treatment of Gaucher disease type 1 (GD1). This multicenter, open‐label, 12‐month study examined the safety and efficacy of velaglucerase alfa in patients with GD1 previously receiving imiglucerase. Eligible patients, ≥2 years old and clinically stable on imiglucerase therapy, were switched to velaglucerase alfa at a dose equal to their prior imiglucerase dose. Infusion durations were 1 hr every other week. Forty patients received velaglucerase alfa (18 male, 22 female; four previously splenectomized; age range 9–71 years). Velaglucerase alfa was generally well tolerated with most adverse events (AEs) of mild or moderate severity. The three most frequently reported AEs were headache (12 of 40 patients), arthralgia (9 of 40 patients), and nasopharyngitis (8 of 40 patients). No patients developed antibodies to velaglucerase alfa. There was one serious AE considered treatment‐related: a Grade 2 anaphylactoid reaction within 30 min of the first infusion. The patient withdrew; this was the only AE‐related withdrawal. Hemoglobin concentrations, platelet counts, and spleen and liver volumes remained stable through 12 months. In conclusion, adult and pediatric patients with GD1, previously treated with imiglucerase, successfully transitioned to velaglucerase alfa, which was generally well tolerated and demonstrated efficacy over 12 months' treatment consistent with that observed in the velaglucerase alfa Phase 3 clinical trial program. Am. J. Hematol. 88:172–178, 2013. © 2012 Wiley Periodicals, Inc.
Effects of Bartonella spp. on Flea Feeding and Reproductive Performance

ABSTRACT Numerous pathogens are transmitted from one host to another by hematophagous insect vectors. The interactions between a vector-borne organism and its vector vary in many ways, most of which are yet to be explored and identified. These interactions may play a role in the dynamics of the infection cycle. One way to evaluate these interactions is by studying the effects of the tested organism on the vector. In this study, we tested the effects of infection with Bartonella species on fitness-related variables of fleas by using Bartonella sp. strain OE 1-1, Xenopsylla ramesis fleas, and Meriones crassus jirds as a model system. Feeding parameters, including blood meal size and metabolic rate during digestion, as well as reproductive parameters, including fecundity, fertility, and life span, were compared between fleas experimentally infected with Bartonella and uninfected fleas. In addition, the developmental time, sex ratio, and body size of F1 offspring fleas were compared between the two groups. Most tested parameters did not differ between infected and uninfected fleas. However, F1 males produced by Bartonella-positive females were significantly smaller than F1 males produced by Bartonella-negative female fleas. The findings in this study suggest that bartonellae are well adapted to their flea vectors, and by minimally affecting their fitness they have evolved to better spread themselves in the natural environment.
Recursion vs Replication in Process Calculi: Expressiveness

In this paper we shall survey and discuss in detail the work on the relative expressiveness of recursion and replication in various process calculi. Namely, CCS, the pi-calculus, and the Ambient calculus. We shall give evidence that the ability of expressing recursive behaviour via replication often depends on the scoping mechanisms of the given calculus which compensate for the restriction of replication.
NTT Neural Machine Translation Systems at WAT 2017

In this year, we participated in four translation subtasks at WAT 2017. Our model structure is quite simple but we used it with well-tuned hyper-parameters, leading to a significant improvement compared to the previous state-of-the-art system. We also tried to make use of the unreliable part of the provided parallel corpus by back-translating and making a synthetic corpus. Our submitted system achieved the new state-of-the-art performance in terms of the BLEU score, as well as human evaluation.
A Broad G Protein-Coupled Receptor Internalization Assay that Combines SNAP-Tag Labeling, Diffusion-Enhanced Resonance Energy Transfer, and a Highly Emissive Terbium Cryptate

Although G protein-coupled receptor (GPCR) internalization has long been considered as a major aspect of the desensitization process that tunes ligand responsiveness, internalization is also involved in receptor resensitization and signaling, as well as the ligand scavenging function of some atypical receptors. Internalization thus contributes to the diversity of GPCR-dependent signaling, and its dynamics and quantification in living cells has generated considerable interest. We developed a robust and sensitive assay to follow and quantify ligand-induced and constitutive-induced GPCR internalization but also receptor recycling in living cells. This assay is based on diffusion-enhanced resonance energy transfer (DERET) between cell surface GPCRs labeled with a luminescent terbium cryptate donor and a fluorescein acceptor present in the culture medium. GPCR internalization results in a quantifiable reduction of energy transfer. This method yields a high signal-to-noise ratio due to time-resolved measurements. For various GPCRs belonging to different classes, we demonstrated that constitutive and ligand-induced internalization could be monitored as a function of time and ligand concentration, thus allowing accurate quantitative determination of kinetics of receptor internalization but also half-maximal effective or inhibitory concentrations of compounds. In addition to its selectivity and sensitivity, we provided evidence that DERET-based internalization assay is particularly suitable for characterizing biased ligands. Furthermore, the determination of a Z′-factor value of 0.45 indicates the quality and suitability of DERET-based internalization assay for high-throughput screening (HTS) of compounds that may modulate GPCRs internalization.
Müller cells express the cannabinoid CB2 receptor in the vervet monkey retina

The presence of the cannabinoid receptor type 1 (CB1R) has been largely documented in the rodent and primate retinae in recent years. There is, however, some controversy concerning the presence of the CB2 receptor (CB2R) within the central nervous system. Only recently, CB2R has been found in the rodent retina, but its presence in the primate retina has not yet been demonstrated. The aim of this study was twofold: 1) to characterize the distribution patterns of CB2R in the monkey retina and compare this distribution with that previously reported for CB1R and 2) to resolve the controversy on the presence of CB2R in the neural component of the retina. We therefore thoroughly examined the cellular localization of CB2R in the vervet monkey (Chlorocebus sabeus) retina, using confocal microscopy. Our results demonstrate that CB2R, like CB1R, is present throughout the retinal layers, but with striking dissimilarities. Double labeling of CB2R and glutamine synthetase shows that CB2R is restricted to Müller cell processes, extending from the internal limiting membrane, with very low staining, to the external limiting membrane, with heavy labeling. We conclude that CB2R is indeed present in the retina but exclusively in the retinal glia, whereas CB1R is expressed only in the neuroretina. These results extend our knowledge on the expression and distribution of cannabinoid receptors in the monkey retina, although further experiments are still needed to clarify their role in retinal functions. J. Comp. Neurol. J. Comp. Neurol. 521:2399–2415, 2013. © 2013 Wiley Periodicals, Inc.
Scavenger receptor CD36 is essential for the cerebrovascular oxidative stress and neurovascular dysfunction induced by amyloid-β

Increasing evidence indicates that cerebrovascular dysfunction plays a pathogenic role in Alzheimer's dementia (AD). Amyloid-β (Aβ), a peptide central to the pathogenesis of AD, has profound vascular effects mediated, for the most part, by reactive oxygen species produced by the enzyme NADPH oxidase. The mechanisms linking Aβ to NADPH oxidase-dependent vascular oxidative stress have not been identified, however. We report that the scavenger receptor CD36, a membrane glycoprotein that binds Aβ, is essential for the vascular oxidative stress and neurovascular dysfunction induced by Aβ1–40. Thus, topical application of Aβ1–40 onto the somatosensory cortex attenuates the increase in cerebral blood flow elicited by neural activity or by endothelium-dependent vasodilators in WT mice but not in CD36-null mice (CD360/0). The cerebrovascular effects of infusion of Aβ1–40 into cerebral arteries are not observed in mice pretreated with CD36 blocking antibodies or in CD360/0 mice. Furthermore, CD36 deficiency prevents the neurovascular dysfunction observed in transgenic mice overexpressing the Swedish mutation of the amyloid precursor protein Tg2576 despite elevated levels of brain Aβ1–40. CD36 is also required for the vascular oxidative stress induced by exogenous Aβ1–40 or observed in Tg2576 mice. These observations establish CD36 as a key link between Aβ1–40 and the NADPH oxidase-dependent vascular oxidative stress underlying the neurovascular dysfunction and suggest that CD36 is a potential therapeutical target to counteract the cerebrovascular dysfunction associated with Aβ.
Diabetogenic Activity of Streptozotocin on Kunming Strain Mice as Animal Model of Diabetes Mellitus

Diabetes mellitus (DM), a chronic metabolic disorder, is increasing tremendously around the world. Assessment of interventions designed to prevent disease progression in humans takes years. On the other hand, the use of plants as diabetic agent needs preclinical test to obtain scientific evidence. Therefore, appropriate experimental animal model of diabetes mellitus is essential tools for understanding the pathogenesis of diabetes mellitus and effectiveness of diabetes phytotherapy. Streptozotocin-induced diabetes is a well-documented model of experimental diabetes. The present study is aimed to investigate the diabetogenic activity of streptozotocin influenced by difference of doses, sex, administration route, duration induction and injection frequency on Kunming strain mice. Induction of STZ on Kunming mice were done according to experimental design and fasting blood glucose level measured using automatic glucometer, in which blood glucose more than 11.1mmol/L is considered as diabetes mellitus. These findings suggest that mild diabetes refers to type 2 diabetes mellitus when fasting blood glucose level is between 11.1-24.9mmol/L. It can be obtained by inducing male Kunming mice using STZ administrated either intravenously at dose of 100 mg/kg or intraperitoneally at dose of 180 mg/kg, in single injection for duration of 3 and 7 days, respectively.
Identification of Gangliosides GD1b and GT1b as Receptors for BK Virus

ABSTRACT Gangliosides have been shown to be plasma membrane receptors for both murine polyomavirus and SV40, while JC virus uses serotonin receptors. In contrast, little is known of the membrane receptor and entry pathway for BK virus (BKV), which can cause severe disease in immunosuppressed bone marrow and renal transplant patients. Using sucrose flotation assays, we investigated BKV binding to and interaction with human erythrocyte membranes and determined that this interaction was dependent on a neuraminidase-sensitive, proteinase K-resistant molecule. BKV was found to interact with the gangliosides GT1b and GD1b. The terminal α2-8-linked disialic acid motif, present in both of these gangliosides, is likely to be important for this interaction. We also determined that the addition of GD1b and GT1b to LNCaP cells, which are normally resistant to BKV infection, made them susceptible to the virus. In addition, BKV interacted with membranes extracted from the endoplasmic reticulum (ER) and infection was blocked by the addition of brefeldin A, which interferes with transport from the ER to the Golgi apparatus. These data demonstrate that BKV uses the gangliosides GT1b and GD1b as receptors and passes through the ER on the way to the nucleus.
A meta‐analysis of the association of adiponectin gene polymorphisms with coronary heart disease in Chinese Han population

Objective Variants of adiponectin gene have been reported to be associated with coronary heart disease (CHD), but the available data on this relationship are inconsistent. A meta‐analysis was performed to quantitatively analyse the association of adiponectin gene polymorphisms with coronary artery disease using previous case–control studies in Chinese Han populations.
Differential Response of QTU Interval to Exercise, Isoproterenol, and Atrial Pacing in Patients with Congenital Long QT Syndrome

Sympathetic stimulation is well known to contribute to the genesis of QTU prolongation and ventricular lachyarrhythmias in patients with congenital long QT syndrome. In this study, we performed exercise treadmill testing, isoproterenol infusion (1–2 μg/min), and right atrial pacing (cycle length 500 msec) in 11 patients with congenital long QT (LQT) syndrome (LQT group) and in 12 age‐ and sex‐matched controls (control group). The responses of the corrected QT (QTc; Bazett's method) interval and the TU wave complex tvere evaluated. The QTc interval was prolonged from 482 ± 63 msec1/2 to 548 ± 28 msec1/2 by exercise in the LQT group (n = 11; P < 0.005), and this was associated with fusion of the T waves with enlarged U waves, whereas the QTc interval did not increase with exercise in the control group (n = 12; 402 ± 19 msec1/2 vs 409 ± 22 msec1/2). The QTc interval was also prolonged from 466 ± 50 msec1/2 to 556 ± 33 msec1/2 by isoproterenol in the LQT group (n = 7; P < 0.005) in association with morphological changes of the TU wave complex like those seen with exercise, whereas it was only slightly increased from 399 ± 10 msec1/2 to 436 ± 13 msec1/2 by isoproterenol in the control group (n = 77; P < 0.001). However, the QTc interval did not increase with atrial pacing in the LQT group (n = 8; 476 ± 57 msec1/2 vs 486 ± 59 msec1/2), whereas it was slightly increased from 400 ± 21 msec1/2 to 426 ± 18 msec1/2 by atrial paring in (he control group (n = 8; P < 0.005). These results suggest that sympathetic stimulation plays an important role in the QTU prolongation and marked TU wave complex abnormalities in patients with congenital long QT syndrome.
Small ribosomal RNA content in marine Proteobacteria during non-steady-state growth.

Nine strains of marine Proteobacteria were assayed for nucleic acid content during non-steady-state growth to assess whether a species-specific growth rate based on rRNA content is feasible for environmental samples. The large and small ribosomal subunits and genomic DNA were quantified using image analysis. It was found that the maximal intracellular concentration of 16S rRNA during batch growth for the bacteria averaged 155 fg+/-60 (S.D.) per cell for eight of the nine marine bacteria in the exponential phase (with the exception of one strain, Pac 218). The dilution/decay of 16S rRNA/cell was rapid with a return to pre-shift up values within 6-12 h for all strains except Vibrio fisherii. An overall relationship between the RNA:DNA ratio and the specific growth rate for non-steady-state growth for all bacterial strains was not observed as previously described for other Proteobacteria during steady-state growth. However, a predictable relationship between rRNA content and growth rate for many isolates during batch growth was observed. Furthermore, the rapid kinetics of intracellular rRNA levels indicates it will be feasible to assess whether specific bacteria are in steady state or non-steady state in the marine environment. If the condition of steady state is met for a specific Proteobacterial group in an environmental sample, it will be possible to estimate species-specific growth rates by measuring rRNA content.