Miracle-dz/UniFAR-FTUs huggingface dataset:
Newly generated neurons in the amygdala and adjoining cortex of adult primates

The subventricular zone remains mitotically active throughout life in rodents. Studies with tritiated thymidine, which is incorporated into the DNA of mitotic cells, have revealed that the rodent subventricular zone produces neuroblasts that migrate toward the olfactory bulb along the rostral migratory stream. A similar migratory stream has been documented in monkeys by using the thymidine analogue BrdUrd. The same approach showed that neurogenesis occurred in the dentate gyrus of adult primates, including humans. In the present study, experiments combining injections of BrdUrd and the dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindo-carbocyanine, with the immunostaining for molecular markers of neurogenesis (polysialylated neural cell adhesion molecule, β-tubulin-III, collapsin response mediator protein-4, neuronal nuclear protein) in New World (Saimiri sciureus) and Old World (Macaca fascicularis) monkeys have revealed that new neurons are produced in the amygdala, piriform cortex, and adjoining inferior temporal cortex in adult primates. These newborn neurons expressed the antiapoptotic protein Bcl-2 and formed a more-or-less continuous pathway that extended from the tip of the temporal ventricular horn to the deep portion of the temporal lobe. The production of newborn neurons in the amygdala, piriform cortex, and inferior temporal cortex seems to parallel the continuing addition of neurons in the olfactory bulb. These two concomitant phenomena may ensure structural stability and functional plasticity to the primate olfactory system and temporal lobe.
Ascertaining Baseline Levels of Antiretroviral Therapy Adherence in Uganda: A Multimethod Approach

Background:Many antiretroviral treatment (ART) adherence measurement methods have been employed by different studies, but no single method has been found to be appropriate for all settings. This study aimed to determine baseline levels of adherence using 2 measures of adherence. Methods:Levels of adherence in 967 patients continuing to receive ART in 4 health facilities were assessed over a 28-week period using a clinic-based pill count method and a patient self-report questionnaire. Factors associated with adherence were also determined. Results:Mean adherence (95% confidence interval) was 97.3% (96.8% to 97.9%) and 98.4% (97.9% to 98.8%) for the clinic-based pill count and patient self-report methods, respectively. Proportion of clients achieving optimal adherence (≥95%) was 89.9% by pill count and 94.2% by self-report. The 2 adherence measures were closely correlated with each other (r = 0.87, P = 0.000). Adherence increased with age (P = 0.014) with patients aged 40 years and below being less likely to achieve optimal adherence [odds ratio = 0.55; 95% confidence interval (0.34 to 0.89)]. Conclusions:There is a very high level of optimal adherence among patients still on treatment. The combined use of these 2 replicable and reliable methods of measuring adherence is vital to ART programs in resource-constrained settings.
Age-related total gray matter and white matter changes in normal adult brain. Part II: quantitative magnetization transfer ratio histogram analysis.

BACKGROUND AND PURPOSE The magnetization transfer ratio (MTR) is a sensitive and quantitative identifier of underlying structural changes in the brain. We quantitatively evaluated age- and sex-related MTR changes in global gray matter (GM) and global white matter (WM) in healthy adults. METHODS Fifty-two healthy volunteers (21 men, 31 women) aged 20-86 years underwent dual-echo fast spin-echo and magnetization transfer imaging performed with and then without a saturation pulse. GM and WM were distinguished by using a computer-assisted semiautomated segmentation technique. MTR histograms were generated for each segmented tissue in each subject and compared among age and sex groups. RESULTS The mean, median, first quartile, and peak height of the MTR histogram were significantly lower in the older group (> or =50 years) than those in the younger group (<50 years) for both GM and WM. The age dependency of these values can be expressed in a quadratic fashion over the entire span of adulthood. The MTRs started to decline only after the age of approximately 40 years in both tissues. No statistically significant differences in MTR histogram measurements between the sexes were observed. CONCLUSION The different MTR values for both GM and WM in the two age groups suggest that notable microscopic changes occur in GM and WM with advancing age, yet no significant sex-related variations in MTR measurements were found in these neurologically healthy adults. Such normative data based on the inherent contrast in MTRs are essential in studies of specific disorders of aging, and they may have implications for our understanding of the gross structural changes in both GM and WM in the aging brain.
Association between Cystic Fibrosis Transmembrane Conductance Regulator Gene Mutations and Susceptibility for Childhood Asthma in Korea

Purpose Classic cystic fibrosis is now known part of cystic fibrosis transmembrane conductance regulator (CFTR)-related disorders. These include a wide spectrum, from multi-system disorders, such as cystic fibrosis, to mono-symptomatic conditions, such as chronic pancreatitis or congenital bilateral absence of the vas deferens. However, respiratory disease is considered typical for the multi system disorder, cystic fibrosis, and is the major cause of morbidity and mortality. The purpose of this study was to evaluate the potential effects of CFTR gene mutations in Korean children with asthma. Materials and Methods We selected 14 mutations identified in Korea and each of the 48 children with and without asthma were genotyped for the case-control study. Results No significant differences were found in genotype and allele frequencies of the 9 polymorphisms observed between the non-asthma and asthma groups. In a haplotype determination based on a Bayesian algorithm, 8 haplotypes were assembled in the 98 individuals tested. However, we also did not find any significant differences in haplotype frequencies between the non-asthma and asthma groups. Conclusion We have concluded that this study did not show any evidence in support of providing that CFTR genetic variations significantly contribute to the susceptibility of asthma in Korean children.
Mid-Holocene horizons of strongly decomposed peat and problems of dating paleohydrological changes in mires in the Racibórz basin, Southern Poland

Abstract During litho-stratigraphic studies of deposits in small mires in the upper basin of the Oder River, mid-Holocene hiatuses identified by the presence of horizons of strongly decomposed peat (HSDP), were identified at four sites. Radiocarbon dating results indicate long (103 years) breaks in peat accumulation and the dates obtained from the underlying layers of deposit are inconsistent with the pollen record. In order to explain these discrepancies, an attempt was made to recreate the conditions of HSDP formation under the influence of endo- and exogenous factors determining peatland productivity. In the light of macrofossil analysis, the development of HSDP during the Atlantic and Subboreal periods, when the humidity of habitats decreased, occurred at an advanced stage of development of swamps with Alnetea glutinosae forest communities. The gradual rise in water levels in the mires that started in the Subboreal period and intensified during the Subatlantic period was recorded as a slow shift in vegetation towards non-forest, sedge-moss communities belonging to the Magnocaricion alliance or the Scheuchzerio-Caricetea nigrae class. One consequence of the mechanism of HSDP formation is the existence of limitations on the dating of the duration of the dry phase owing to the presence of organic matter of different ages in the degraded peat layer. The determination of the age of wet phases offers better prospects. In the light of radiocarbon dating, which is essentially in line with the pollen analysis results, the transition towards positive peatland productivity occurred between ca. 9th century BC and 7th century CE.
Anorexia athletica in pre-professional ballet dancers

Abstract Competitive sport has been under increasing discussion as a possible favourable factor in the development of eating disorders among children and adolescents. The aim of this study was to determine the frequency of sport-specific eating disorders, in line with the concept of anorexia athletica. This prospective field study included one experimental group and two control groups (disease and healthy). Fifty-two pre-professional ballet dancers aged 13–20 years were tested for clinical eating disorders, anorexia athletica criteria, eating disorder related psychopathology and self-concept, and were compared with 52 patients with anorexia nervosa and 44 non-athletic controls of the same age. The study was conducted using semi-structured interviews as well as self-report questionnaires. A clinical eating disorder diagnosis was made in 1.9% of the ballet dancers versus 0% of the high school students; anorexia athletica was diagnosed in 5.8% of the dancers versus 2.3% of the students. Ballet dancers scored lower than patients with anorexia nervosa with regard to eating disorder related psychopathology and higher than the patients with regard to self-concept. We conclude that more sensitive tools to differentiate between sport-specific (eating) patterns, anorexia athletica and clinically relevant eating disorders are needed, especially for aesthetic sports such as ballet. It remains an important goal to identify athletes with symptoms of anorexia athletica irrespective of their physique and/or sport.
Infarction Distribution Pattern in Acute Stroke May Predict the Extent of Leptomeningeal Collaterals

Objective The aim of this study was to evaluate whether the distribution pattern of early ischemic changes in the initial MRI allows a practical method for estimating leptomeningeal collateralization in acute ischemic stroke (AIS). Methods Seventy-four patients with AIS underwent MRI followed by conventional angiogram and mechanical thrombectomy. Diffusion restriction in Diffusion weighted imaging (DWI) and correlated T2-hyperintensity of the infarct were retrospectively analyzed and subdivided in accordance with Alberta Stroke Program Early CT score (ASPECTS). Patients were angiographically graded in collateralization groups according to the method of Higashida, and dichotomized in 2 groups: 29 subjects with collateralization grade 3 or 4 (well-collateralized group) and 45 subjects with grade 1 or 2 (poorly-collateralized group). Individual ASPECTS areas were compared among the groups. Results Means for overall DWI-ASPECTS were 6.34 vs. 4.51 (well vs. poorly collateralized groups respectively), and for T2-ASPECTS 9.34 vs 8.96. A significant difference between groups was found for DWI-ASPECTS (p<0.001), but not for T2-ASPECTS (p = 0.088). Regarding the individual areas, only insula, M1-M4 and M6 showed significantly fewer infarctions in the well-collateralized group (p-values <0.001 to 0.015). 89% of patients in the well-collateralized group showed 0–2 infarctions in these six areas (44.8% with 0 infarctions), while 59.9% patients of the poor-collateralized group showed 3–6 infarctions. Conclusion Patients with poor leptomeningeal collateralization show more infarcts on the initial MRI, particularly in the ASPECTS areas M1 to M4, M6 and insula. Therefore DWI abnormalities in these areas may be a surrogate marker for poor leptomeningeal collaterals and may be useful for estimation of the collateral status in routine clinical evaluation.
Activity of panitumumab alone or with chemotherapy in non-small cell lung carcinoma cell lines expressing mutant epidermal growth factor receptor

Epidermal growth factor receptor (EGFR) kinase domain mutations cause hyperresponsiveness to ligand and hypersensitivity to small-molecule tyrosine kinase inhibitors. However, little is known about how these mutations respond to antibodies against EGFR. We investigated the activity of panitumumab, a fully human anti-EGFR monoclonal antibody, in vitro in mutant EGFR-expressing non-small cell lung carcinoma (NSCLC) cells and in vivo with chemotherapy in xenograft models. Mutant EGFR-expressing NSCLC cells (NCI-H1975 [L858R+T790M] and NCI-H1650 [Δ746-750]) and CHO cells were treated with panitumumab before EGF stimulation to assess the inhibition of EGFR autophosphorylation. Established tumors were treated with panitumumab (25, 100, or 500 μg/mouse twice a week) alone or with docetaxel (10 or 20 mg/kg once a week) or cisplatin (7.5 mg/kg once a week). Antitumor activity and levels of proliferation markers were analyzed. Treatment of mutant EGFR-expressing CHO and NSCLC cells with panitumumab inhibited ligand-dependent autophosphorylation. In NCI-H1975 and NCI-H1650 xenografts, treatment with panitumumab alone or with cisplatin inhibited tumor growth compared with control (P < 0.0003). With panitumumab plus docetaxel, enhanced antitumor activity was seen in both xenografts versus panitumumab alone. Panitumumab treatment alone decreased Ki-67 and phospho- mitogen-activated protein kinase (pMAPK) staining in both xenografts compared with control. Docetaxel enhanced panitumumab activity in NCI-H1650 xenografts (decreased Ki-67 and pMAPK staining by >60%) when compared with either agent alone. Panitumumab inhibits ligand-induced EGFR phosphorylation, tumor growth, and markers of proliferation alone or with docetaxel in NSCLC cell lines that express clinically observed EGFR kinase domain mutations, including the small-molecule tyrosine kinase inhibitor-resistant T790M mutation. [Mol Cancer Ther 2009;8(6):1536–46]
DEM Resolution Effects on Coastal Flood Vulnerability Assessment: Deterministic and Probabilistic Approach

Flood modeling is highly influenced by topography data set. Lower resolution digital elevation models (DEMs) are usually used because of their availability and less computational burden when they are used in modeling applications. However, low accuracy of these DEMs yields to even lower accuracy in flood risk analysis through spatial modeling. This study aims to explore the DEM resolution effects on coastal flood risk assessments. For this purpose, deterministic and probabilistic approaches are employed for flood inundation modeling utilizing hydrologically connected bathtub method. High‐resolution light detection and ranging (LiDAR) DEM is considered as the most accurate data from which different resolution maps are obtained using resampling techniques. This is incorporated into an error analysis framework along with U.S. Geological Survey (USGS) National Elevation Dataset (NED) DEMs. The probabilistic framework is developed by simulating the spatial variability of elevation errors compared to LiDAR DEM through a Monte Carlo‐based method called sequential Gaussian simulation. The proposed methodology is applied to the lower Manhattan in New York City. By integrating the flood model into the developed framework, flood inundation probability at each grid cells is obtained. Furthermore, using the concept of accuracy‐efficiency tradeoffs, a framework for selecting a suitable spatial resolution for probabilistic flood risk assessment has been suggested. The results show that by exercising a range of options presented in this paper, a broader insight into mapping resolution can be provided, improving flood assessment, evacuation zones, and mitigation plans depending upon the data availability in a region.
Estimation of a k-monotone density: limit distribution theory and the Spline connection

We study the asymptotic behavior of the Maximum Likelihood and Least Squares Estimators of a kmonotone density g0 at a fixed point x0 when k> 2. We find that the jth derivative of the estima- tors at x0 converges at the rate n −(k−j)/(2k+1) for j =0 ,. ,k � 1. The limiting distribution depends on an almost surely uniquely de- fined stochastic process Hk that stays above (below) the k-fold in- tegral of Brownian motion plus a deterministic drift, when k is even (odd). Both the MLE and LSE are known to be splines of degree k � 1 with simple knots. Establishing the order of the random gap τ + n � τ − n where τ ± n denote two successive knots, is a key ingredient of the proof of the main results. We show that this "gap problem" can be solved if a conjecture about the upper bound on the error in a particular Hermite interpolation via odd-degree splines holds.
Ca2+-sensing Receptor Cleavage by Calpain Partially Accounts for Altered Vascular Reactivity in Mice Fed a High-fat Diet

Abstract: The Ca2+-sensing receptor (CaSR) is expressed in endothelial and smooth muscle cells, but its role in regulating vascular reactivity is unclear, as are the effects of disease on CaSR function and expression. We studied vascular reactivity in aortic segments from healthy and diabetic mice, combined with in vitro proteolysis studies and Western blot analyses of CaSR expression in tissue samples. In endothelium-intact aortic rings, extracellular Ca2+ elicited a nitric oxide–dependent relaxation that was attenuated by the CaSR antagonist, NPS2390. The calcimimetic, calindol, induced the endothelium-independent relaxation of aortic segments that was also sensitive to NPS2390. The antagonist failed to affect responses to acetylcholine or U46619 but attenuated contractions to phenylephrine and potassium. In mice fed a Western-type diet, phenylephrine-induced contractions and calindol-induced relaxations were markedly attenuated, and CaSR expression was decreased. The latter phenomenon could be attributed to the activation of the Ca2+-dependent protease, µ-calpain, and the subsequent proteolytic cleavage of the CaSR. CaSR activation in smooth muscle cells modulates vascular responsiveness to Ca2+-elevating agonists. These effects are blunted during metabolic stress because of the limited proteolysis of the CaSR by calpain. The loss of the CaSR function may predispose to the macrovascular late complications associated with diabetes.
Prevalence of Latent Tuberculosis Infection and Associated Risk Factors Among 1557 Nursing Students in a Context of Low Endemicity

Introduction: The risk of tuberculosis (TBC) in nurses is related to its incidence in the general population. Nursing students involved in clinical training could be exposed to occupational risks similar to those of healthcare workers (HCWs). To better understand the epidemiology of nosocomial TBC among nurses in a context of low endemicity, we recruited a cohort of young nursing trainees at the Second University of Naples. Methods: A screening programme for LTBI in nursing students was conducted between January 2012 and December 2013, at the Second University of Naples, with clinical evaluations, tuberculin skin test (TST) and, in positive TST student, the interferon-g release assays (IGRA). Putative risk factors for LTBI were assessed by a standardized questionnaire. Results: 1577 nursing students attending the Second University of Naples have been submitted to screening programme for TBC. 1575 have performed TST as first level test and 2 Quantiferon test (QFT). 19 students were TST positive and continued the diagnostic workup practicing QFT, that was positive in 1 student. Of the 2 subjects that have practiced QFT as first level test only 1 was positive. In 2 students positive to QFT test we formulated the diagnosis of LTBI by clinical and radiographic results. Conclusion: The prevalence of LTBI among nursing students in our study resulted very low. In countries with a low incidence of TBC, the screening programs of healthcare students can be useful for the early identification and treatment of the sporadic cases of LTBI.
Responses

This essay is a response to the excellent contributions to the double special issue of Law and Philosophy on my book The Ends of Harm. I further defend the Duty View of punishment outlined in the book, responding to criticisms of that view. I also challenge the plausibility of retributivist accounts offered in response to the challenges to that view developed in The Ends of Harm.
Nitrification Inhibition Using Benzotriazoles

ABSTRACT The common corrosion inhibitor, 1H-benzotriazole (Bz), found as a component of glycol-based aircraft deicers, and its derivatives such as 5-chloro-1H-benzotriazole (CBz), 1-hydroxy-benzotriazole (HBz), and 5-methyl-1H-benzotriazole (MBz), are all potential nitrification inhibitors. The inhibitory effect on the nitrification of urea-N fertilizer in agricultural soils was examined over a four-week period. All the compounds tested, except HBz, have the ability to inhibit the nitrification of urea-N fertilizer effectively. Their inhibitory capabilities depend on the structural components of the soil, particularly organic matter (O.M.). In the soil with the largest percent of O.M. (2.3%), inhibition by all the benzotriazole derivatives was considerably decreased. In the soil with least O.M. (0.3%), even HBz, the poorest of the inhibitors, showed signs of inhibition. For Bz, MBz, and CBz, in the range of 90% inhibition was observed in the soil with the lowest O.M. content during a four-week treatment period. On a mass basis, Bz had the greatest inhibitory effect followed by MBz and CBz, whereas HBz showed little inhibition of the nitrification of ureaN in soils with >0.5% O.M. If the inhibitory effects were looked at on a molar concentration basis, there would be little variation in inhibitory potency for MBz, CBz, and Bz. It was concluded that all three are effective nitrification inhibitors with urea-N fertilizer. The incorporation of benzotriazoles as inhibitors could help economize N fertilizers by helping prevent leaching and denitrification. However, the environmental fate of these compounds has not been determined, and they may produce toxicity to plants and other organisms.
CollabLoc: Privacy-Preserving Multi-Modal Localization via Collaborative Information Fusion

Mobile phones provide an excellent opportunity for building context-aware applications. In particular, location-based services are important context-aware services that are more and more used for enforcing security policies, for supporting indoor room navigation, and for providing personalized assistance. However, a major problem still remains unaddressed--the lack of solutions that work across buildings while not using additional infrastructure and also accounting for privacy and reliability needs. In this paper, a privacy-preserving, multi-modal, cross-building, collaborative localization platform is proposed based on Wi-Fi RSSI (existing infrastructure), Cellular RSSI, sound and light levels, that enables room-level localization as main application (though sub room level granularity is possible). The privacy is inherently built into the solution based on onion routing, and perturbation/randomization techniques, and exploits the idea of weighted collaboration to increase the reliability as well as to limit the effect of noisy devices (due to sensor noise/privacy). The proposed solution has been analyzed in terms of privacy, accuracy, optimum parameters, and other overheads on location data collected at multiple indoor and outdoor locations.