Miracle-dz/UniFAR-FTUs huggingface dataset:
Zone Specific Fractal Dimension of Retinal Images as Predictor of Stroke Incidence

Fractal dimensions (FDs) are frequently used for summarizing the complexity of retinal vascular. However, previous techniques on this topic were not zone specific. A new methodology to measure FD of a specific zone in retinal images has been developed and tested as a marker for stroke prediction. Higuchi's fractal dimension was measured in circumferential direction (FDC) with respect to optic disk (OD), in three concentric regions between OD boundary and 1.5 OD diameter from its margin. The significance of its association with future episode of stroke event was tested using the Blue Mountain Eye Study (BMES) database and compared against spectrum fractal dimension (SFD) and box-counting (BC) dimension. Kruskal-Wallis analysis revealed FDC as a better predictor of stroke (H = 5.80, P = 0.016, α = 0.05) compared with SFD (H = 0.51, P = 0.475, α = 0.05) and BC (H = 0.41, P = 0.520, α = 0.05) with overall lower median value for the cases compared to the control group. This work has shown that there is a significant association between zone specific FDC of eye fundus images with future episode of stroke while this difference is not significant when other FD methods are employed.
The Estimation of the Equilibrium Real Exchange Rate for Romania

This paper aims to estimate the equilibrium real exchange rate for Romania, respectively the real exchange rate consistent with the macroeconomic balance, which is achieved when the economy is operating at full employment and low inflation (internal balance) and has a current account that is sustainable (external balance). This equilibrium real exchange rate is very important for an economy because deviations of the real exchange rate from its equilibrium value can affect the competitiveness of a country. An overvalued real exchange rate will determine a lack of external competitiveness and deteriorate the country’s real activity. An undervalued exchange rate will increase on short term exports and it will lower the current account deficit but, on the long term it will increase the inflationary pressures. The equilibrium real exchange rate is also a very important variable for a country who wishes to join ERM II. In fact the central parity should be chosen to reflect the equilibrium exchange rate. The conclusion is that the real exchange rate had some important deviations from its equilibrium value which were determined by the liberalization of the prices and of the foreign exchange market and by the fluctuations of the nominal exchange rate. These deviations are not likely to put at risk the entry in ERM II.
Social determinants of the use of health services among a public university workers.

OBJECTIVE To analyze the use of health services and socioeconomic status among a public university workers. METHODS A cross-sectional study with 759 workers at a Brazilian public university who reported health-related restrictions of their usual activities in the previous 14 days, was carried out. Data were supplied by the 2001 cohort of the "Pró-Saúde Study" in Rio de Janeiro, Southeastern Brazil. Health services use was assessed with a proxy for "seeking health care" and according to the type of service. The presence of additional variation in morbidity was verified by time restriction. Schooling, income and occupation markers were analyzed, and crude and adjusted proportion ratios of use and types of service were calculated. RESULTS The occupation level was the indicator of the greatest inequality in health services use. After adjustments for gender, age and the other socioeconomic status markers, the ratio of the proportion of health care use was 1.31 for manual workers (95%CI: 1.11;1.55) and 1.21 for non-manual workers (95%CI: 1.06;1.37) compared to the reference category of professionals. CONCLUSIONS A pattern of social inequality was identified in health services use. Even after an adjustment for health need, the pattern favored individuals with lower socioeconomic status, particularly for the occupation marker. Remaining differences in individual morbidities do not explain this finding. Rather, occupational factors may exert a greater influence on health services use in this population.
BGP Prefix Delegations: A Deep Dive

Among the scalability limits in the Internet routing ecosystem are the BGP routing table size and the rate of updates. In this paper, we focus on the former. The BGP routing table size has been growing superlinearly in the past decades. Although the address space allocation was structured with aggregation in mind, this capability is impaired due in part to the need for traffic engineering. One major impairment are delegated prefixes. Prefix delegations contribute 15% to the routing table size and they also involve 14% of the traffic. Moreover, they reflect the large complexity of BGP: Delegations are not only the results of providers delegating address space to their customers but also vice versa. Some delegations are due to load balancing and transitive business relationships, others due to operational practices within organizations. We analyze BGP data as well as large-scale traceroute data to understand how delegations affect path selection. Although the need for delegations is apparent, our work highlights that the fragmentation of address space is going to continue and renders the routing table more and more un-aggregatable.
Gender differences, risk attitude and entrepreneurship in Kyrgyzstan

This study analyses the role of risk attitude for entrepreneurship by gender differences in Kyrgyzstan. Logit analysis is applied to the cross-sectional data set drawn from the nationally representative survey for 2011. Entrepreneurship is measured by the self-employment activities and analysed by the agricultural and non-agricultural sample. Results of the study show that more risk-taking preferences are associated with higher entrepreneurship probability. However, this effect is not persistent for women in further estimations for non-agricultural entrepreneurship sample, while for men higher positive effect of risk loving behavior remains in off-farm self-employment too. These findings underline the existing difference in risk tolerance by gender in non-agricultural employment. Movement of women from farm to off-farm entrepreneurship may not necessarily require risk loving characteristics. However, further analysis of this difference should take into account potential difference of necessity and opportunity entrepreneurs by gender.
Active DC Voltage Balancing PWM Technique for High-Power Cascaded Multilevel Converters

In this paper, a dedicated pulse width modulation (PWM) technique specifically designed for single-phase (or four wire three-phase) multilevel Cascaded H-Bridge Converters is presented. The aim of the proposed technique is to minimize the DC-Link voltage unbalance, independently from the amplitude of the DC-Link voltage reference, and compensate the switching device voltage drops and on-state resistances. Such compensation can be used to achieve an increase in the waveform quality of the converter. This is particularly useful in high-power low supply voltage applications where a low switching frequency is used. The DC-Link voltage balancing capability of the method removes the requirement for additional control loops to actively balance the DC-Link voltage on each H-Bridge, simplifying the control structure. The proposed modulation technique has been validated through the use of simulation and extensive experimental testing to confirm its effectiveness.
A Description of Female Genital Mutilation and Force-Feeding Practices in Mauritania: Implications for the Protection of Child Rights and Health

Objectives To establish the prevalence of female genital mutilation (FGM) and force feeding (gavage) practices among children in Mauritania; to investigate factors related to FGM and gavage practices and attitude in Mauritania; and to explore implications related to the protection of children’s rights and welfare. Methods Data from the Mauritania 2000–2001 DHS were used in this analysis. Data were collected from men and women about their attitude toward the continuation of FGM and gavage; women only were asked if they ever experienced one of these practices. Chi-square statistics were used to investigate differences in attitude and practice of FGM and gavage by demographic characteristics. Binary logistic regression was used to identify socio-demographic factors related to FGM and gavage outcomes. Findings The overall prevalence of FGM was 77% but varied depending on ethnicity. The majority of both female and male respondents favored the continuation of the practice (64% and 70%, respectively). Almost a quarter (23%) of women reported being force fed as a child and 32% of women and 29% of men approved the continuation of the practice. Gavage is almost exclusively practiced among Arabs. Conclusion The practice of both FGM and gavage is ongoing, although the prevalence and attitude towards both appears to vary as a function of ethnicity, wealth, education, marital status, and age. Contextually relevant intervention and enforcement strategies are needed to challenge these cultural norms and protect the rights and welfare of children in Mauritania.
A Low-Level Generalization Model for Simulating Global Economic Meltdown

The managing of a financial crisis of global magnitude requires a low-level generalization for an open intervention program to materialize. This paper presents an open system for simulating and/or monitoring scenarios of financial crises.The method employed involves the use of a ‘thinking’ chart to document significant theories of the economy for the purpose of pragmatic analysis. Subsequently, a system’s perspective of the real economy is used to underscore a coterie of models and observations about the economy. The results were validated as the basis for unifying the outlooks of the current state of global economies. In conclusion, the current financial crisis is fundamentally a fuel crisis based on a low-level generalization. This was an observable pattern that had not been taken into full dialectical partnership, and, hence, obscured the socio-economic reading of history in its time, enthroning the mutant in the realm of human ideas.
Towards a taxonomy of parallel branch and bound algorithms

textabstractIn this paper we present a classification of parallel branch and bound algorithms, and elaborate on the consequences of particular parameter settings. The taxonomy is based upon how the algorithms handle the knowledge about the problem instance to be solved, generated during execution. The starting point of the taxonomy is the generally accepted description of the sequential branch and bound algorithm, as presented in, for example, [Mitten 1970] and [Ibaraki 1976a, 1976b, 1977a, 1977b].
Trophoblastic invasion in vitro and in vivo: similarities and differences.

BACKGROUND The basic mechanisms of trophoblast invasion are not completely understood. This may be due to the lack of suitable in vitro models which enable experimental modulation of this complex process. In the present study, a three-dimensional co-culture model is used for comparing two factors considered to be implicated in the regulation of trophoblast invasion, the expression of HLA-G and apoptosis, in vitro and in vivo. METHODS Tissue fragments from human first trimester decidua parietalis were put in close contact with spheroids of AC-1M59 trophoblast/choriocarcinoma hybrid cells as a model of the invasive trophoblast. Cryostat sections from these co-cultures were immunohistochemically stained and compared with first trimester placentation sites in vivo. RESULTS Only the invasive trophoblast-derived cells showed an intensive staining for HLA-G, whereas the cells on the periphery of the confrontation culture exhibited only a weak staining. A similar staining pattern was found in vivo. Both in vitro and in vivo CD45(+) apoptotic leukocytes were frequently detected in close proximity to the invasive trophoblastic cells. CONCLUSIONS In this co-culture system, key factors considered to be implicated in trophoblast invasion in vivo can also be demonstrated in vitro. Therefore, it may help in finding strategies for the management of diseases associated with deficient trophoblast invasion.
Implementing Scalable, Network-Aware Virtual Machine Migration for Cloud Data Centers

Virtualization has been key to the success of Cloud Computing through the on-demand allocation of shared hardware resources to Virtual Machines (VM)s. However, the network-agnostic placement of VMs over the underlying network topology can itself be a factor of performance degradation by causing congestion at the core layers of the infrastructure where bandwidth is heavily oversubscribed. In this paper, we design and implement S-CORE, a scalable live VM migration scheme to dynamically reallocate VMs to servers while minimizing the overall communication footprint of active traffic flows. We evaluate S- CORE over diverse aggregate load and coordination policies. Our results show that it can achieve up to a 87% communication cost reduction with a limited number of migration rounds, and can be easily accommodated within commodity hardware and hypervisor architectures. The associated memory, CPU, and network overhead are also minimum under typical Cloud Data Center workloads.
Genomic and Epigenomic Integration Identifies a Prognostic Signature in Colon Cancer

Purpose: The importance of genetic and epigenetic alterations maybe in their aggregate role in altering core pathways in tumorigenesis. Experimental Design: Merging genome-wide genomic and epigenomic alterations, we identify key genes and pathways altered in colorectal cancers (CRC). DNA methylation analysis was tested for predicting survival in CRC patients using Cox proportional hazard model. Results: We identified 29 low frequency-mutated genes that are also inactivated by epigenetic mechanisms in CRC. Pathway analysis showed the extracellular matrix (ECM) remodeling pathway is silenced in CRC. Six ECM pathway genes were tested for their prognostic potential in large CRC cohorts (n = 777). DNA methylation of IGFBP3 and EVL predicted for poor survival (IGFBP3: HR = 2.58, 95% CI: 1.37–4.87, P = 0.004; EVL: HR = 2.48, 95% CI: 1.07–5.74, P = 0.034) and simultaneous methylation of multiple genes predicted significantly worse survival (HR = 8.61, 95% CI: 2.16–34.36, P < 0.001 for methylation of IGFBP3, EVL, CD109, and FLNC). DNA methylation of IGFBP3 and EVL was validated as a prognostic marker in an independent contemporary-matched cohort (IGFBP3 HR = 2.06, 95% CI: 1.04–4.09, P = 0.038; EVL HR = 2.23, 95% CI: 1.00–5.0, P = 0.05) and EVL DNA methylation remained significant in a secondary historical validation cohort (HR = 1.41, 95% CI: 1.05–1.89, P = 0.022). Moreover, DNA methylation of selected ECM genes helps to stratify the high-risk stage 2 colon cancers patients who would benefit from adjuvant chemotherapy (HR: 5.85, 95% CI: 2.03–16.83, P = 0.001 for simultaneous methylation of IGFBP3, EVL, and CD109). Conclusions: CRC that have silenced genes in ECM pathway components show worse survival suggesting that our finding provides novel prognostic biomarkers for CRC and reflects the high importance of integrative analyses linking genetic and epigenetic abnormalities with pathway disruption in cancer. Clin Cancer Res; 17(6); 1535–45. ©2011 AACR.
Experimental support for Townsend’s Reynolds number similarity hypothesis on rough walls

The Reynolds number similarity hypothesis of Townsend [The Structure of Turbulent Shear Flow (Cambridge University Press, Cambridge, UK, 1976)] states that the turbulence beyond a few roughness heights from the wall is independent of the surface condition. The underlying assumption is that the boundary layer thickness δ is large compared to the roughness height k. This hypothesis was tested experimentally on two types of three-dimensional rough surfaces. Boundary layer measurements were made on flat plates covered with sand grain and woven mesh roughness in a closed return water tunnel at a momentum thickness Reynolds number Reθ of ∼14000. The boundary layers on the rough walls were in the fully rough flow regime (ks+⩾100) with the ratio of the boundary layer thickness to the equivalent sand roughness height δ∕ks greater than 40. The results show that the mean velocity profiles for rough and smooth walls collapse well in velocity defect form in the overlap and outer regions of the boundary layer. The Reyn.
The efficiency of male fertility restoration is dependent on the recovery kinetics of spermatogonial stem cells after cytotoxic treatment with busulfan in mice.

BACKGROUND Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis and represent a crucial resource for male fertility restoration. It has not been well documented, however, whether the recovery of SSC population size after cytotoxic damage associates with the kinetics of male fertility restoration. We addressed this issue using the mouse as a model. METHODS Following single injections of busulfan at 15, 30 or 45 mg/kg into male mice, we examined their ability to sire offspring at different times by natural mating and determined SSC numbers using spermatogonial transplantation. We measured testis physiological parameters (testis weights, sperm counts, serum and intratesticular testosterone levels, and histological assessments of spermatogenic recovery) and quantified the expression of glial-cell-line-derived neurotrophic factor (GDNF) transcripts. RESULTS Regardless of busulfan doses, fertility was lost within 4 weeks after treatment, while more than 95% of SSCs were lost within 3 days. Fertility and SSC numbers gradually recovered with time, but the recoveries were delayed at higher busulfan doses. Interestingly, SSC numbers reached ∼30% of before-treatment levels by 4 weeks prior to the time of fertility restoration, across the dose groups. Sperm counts were ∼20% of before-treatment levels at the onset of fertility restoration, regardless of busulfan doses. We detected a significant increase in total GDNF mRNA per testis immediately after busulfan treatment. CONCLUSIONS The loss and restoration of fertility after busulfan treatment are direct consequences of SSC loss and expansion. Our data suggest that there is a threshold in SSC numbers that allows for male fertility restoration and that the testicular somatic environment responds rapidly and temporarily to the loss of spermatogonia, including SSCs, by altering GDNF mRNA levels. This study provides fundamental information to clinically apply SSCs for male fertility restoration in the future.
Mitochondrial Phosphoproteome Revealed by an Improved IMAC Method and MS/MS/MS*S

IMAC in combination with mass spectrometry is a promising approach for global analysis of protein phosphorylation. Nevertheless this approach suffers from two shortcomings: inadequate efficiency of IMAC and poor fragmentation of phosphopeptides in the mass spectrometer. Here we report optimization of the IMAC procedure using 32P-labeled tryptic peptides and development of MS/MS/MS (MS3) for identifying phosphopeptide sequences and phosphorylation sites. The improved IMAC method allowed recovery of phosphorylated tryptic peptides up to ∼77% with only minor retention of unphosphorylated peptides. MS3 led to efficient fragmentation of the peptide backbone in phosphopeptides for sequence assignment. Proteomics of mitochondrial phosphoproteins using the resulting IMAC protocol and MS3 revealed 84 phosphorylation sites in 62 proteins, most of which have not been reported before. These results revealed diverse phosphorylation pathways involved in the regulation of mitochondrial functions. Integration of the optimized batchwise IMAC protocol with MS3 offers a relatively simple and more efficient approach for proteomics of protein phosphorylation.
A Comparative Analysis of the Molecular Features of MANF and CDNF

Cerebral dopamine neurotrophic factor (CDNF) is a paralogous protein of mesencephalic astrocyte-derived neurotrophic factor (MANF). Both proteins have been reported to show a common cytoprotective effect on dopaminergic neurons as a secretory protein containing the KDEL-like motif of the ER retrieval signal at the C-terminus, RTDL in MANF and [Q/K]TEL in CDNF among many species, although functions of paralogous proteins tend to differ from each other. In this study, we focused on post-translational regulations of their retention in the endoplasmic reticulum (ER) and secretion and performed comparative experiments on characterization of mouse MANF and mouse CDNF according to our previous report about biosynthesis and secretion of mouse MANF using a NanoLuc system. In this study, co-expression of glucose-regulated protein 78 kDa (GRP78), KDEL receptor 1 or mutant Sar1 into HEK293 cells similarly decreased MANF and CDNF secretion with some degree of variation. Next, we investigated whether CDNF affects the secretion of mouse cysteine-rich with EGF-like domains 2 (CRELD2) because mouse wild-type (wt) MANF but not its KDEL-like motif deleted mutant (ΔCMANF) was found to promote the CRELD2 release from the transfected cells. Co-expressing CRELD2 with wt or ΔC CDNF, we found that CDNF and ΔCMANF hardly elevated the CRELD2 secretion. We then investigated effects of the four or six C-terminal amino acids of MANF and CDNF on the CRELD2 secretion. As a result, co-transfection of mouse CDNF having the mouse MANF-type C-terminal amino acids (CDNFRTDL and CDNFSARTDL) increased the CRELD2 secretion to a small extent, but mouse CDNF having human CDNF-type ones (CDNFKTEL and CDNFHPKTEL) well increased the CRELD2 secretion. On the other hand, the replacement of C-terminal motifs of mouse MANF with those of mouse CDNF (MANFQTEL and MANFYPQTEL) enhanced the CRELD2 secretion, and the mouse MANF having human CDNF-type ones (MANFKTEL and MANFHPKTEL) dramatically potentiated the CRELD2 secretion. These results indicate that the secretion of mouse MANF and mouse CDNF is fundamentally regulated in the same manner and that the variation of four C-terminal amino acids in the MANF and CDNF among species might influence their intracellular functions. This finding could be a hint to identify physiological functions of MANF and CDNF.
Diurnal Oscillation of Amygdala Clock Gene Expression and Loss of Synchrony in a Mouse Model of Depression

Background: Disturbances in circadian rhythm-related physiological and behavioral processes are frequently observed in depressed patients and several clock genes have been identified as risk factors for the development of mood disorders. However, the particular involvement of the circadian system in the pathophysiology of depression and its molecular regulatory interface is incompletely understood. Methods: A naturalistic animal model of depression based upon exposure to chronic mild stress was used to induce anhedonic behavior in mice. Micro-punch dissection was used to isolate basolateral amygdala tissue from anhedonic mice followed by quantitative real-time PCR–based analysis of gene expression. Results: Here we demonstrate that chronic mild stress-induced anhedonic behavior is associated with disturbed diurnal oscillation of the expression of Clock, Cry2, Per1, Per3, Id2, Rev-erbα, Ror-β and Ror-γ in the mouse basolateral amygdala. Clock gene desynchronization was accompanied by disruption of the diurnal expressional pattern of vascular endothelial growth factor A expression in the basolateral amygdala of anhedonic mice, also reflected in alterations of circulating vascular endothelial growth factor A levels. Conclusion: We propose that aberrant control of diurnal rhythmicity related to depression may indeed directly result from the illness itself and establish an animal model for the further exploration of the molecular mechanisms mediating the involvement of the circadian system in the pathophysiology of mood disorders.