Miracle-dz/UniFAR-FTUs huggingface dataset:
An MRI Study of Symptomatic Adhesive Capsulitis

Background Appilication of MR imaging to diagnose Adhesive Capsulitis (AC) has previously been described. However, there is insufficient information available for the MRI analysis of AC. This study is to describe and evaluate the pathomorphology of the shoulder in Asian patients with AC compared to healthy volunteers. Methodology/Principal Findings 60 Asian patients with clinically diagnosed AC and 60 healthy volunteers without frozen shoulder underwent MRI of the shoulder joint. All subjects who were age- and sex-matched control ones underwent routine MRI scans of the affected shoulder, including axial, oblique coronal, oblique sagittal T1WI SE and coronal oblique T2WI FSE sequences. Significant abnormal findings were observed on MRI, especially at the rotator cuff interval. The coracohumeral ligament (CHL), articular capsule thickness in the rotator cuff interval as well as the fat space under coracoid process were evaluated. MRI showed that patients with adhesive capsulitis had a significantly thickened coracohumeral ligament and articular capsule in the rotator cuff interval compared to the control subjects (4.2 vs. 2.4 mm, 7.2 vs. 4.4 mm; p<0.05). Partial or complete obliteration of the subcoracoid fat triangle was significantly more frequent in patients with adhesive capsulitis compared with control subjects (73% vs. 13%, 26% vs. 1.6%; p<0.001). Synovitis-like abnormality around the long biceps tendon was significantly more common in patients with adhesive capsulitis than in control subjects. With regards to the inter-observer variability, two MR radiologists had an excellent kappa value of 0.86. Conclusions/Significance MRI can be used to show characteristic findings in diagnosing AC. Thickening of the CHL and the capsule at the rotator cuff interval and complete obliteration of the fat triangle under the coracoid process have been shown to be the most characteristic MR findings seen with AC.
Sketch-pix2seq: a Model to Generate Sketches of Multiple Categories

Sketch is an important media for human to communicate ideas, which reflects the superiority of human intelligence. Studies on sketch can be roughly summarized into recognition and generation. Existing models on image recognition failed to obtain satisfying performance on sketch classification. But for sketch generation, a recent study proposed a sequence-to-sequence variational-auto-encoder (VAE) model called sketch-rnn which was able to generate sketches based on human inputs. The model achieved amazing results when asked to learn one category of object, such as an animal or a vehicle. However, the performance dropped when multiple categories were fed into the model. Here, we proposed a model called sketch-pix2seq which could learn and draw multiple categories of sketches. Two modifications were made to improve the sketch-rnn model: one is to replace the bidirectional recurrent neural network (BRNN) encoder with a convolutional neural network(CNN); the other is to remove the Kullback-Leibler divergence from the objective function of VAE. Experimental results showed that models with CNN encoders outperformed those with RNN encoders in generating human-style sketches. Visualization of the latent space illustrated that the removal of KL-divergence made the encoder learn a posterior of latent space that reflected the features of different categories. Moreover, the combination of CNN encoder and removal of KL-divergence, i.e., the sketch-pix2seq model, had better performance in learning and generating sketches of multiple categories and showed promising results in creativity tasks.
Prey Capture by Larval Zebrafish: Evidence for Fine Axial Motor Control

Swimming and turning behaviors of larval zebrafish have been described kinematically, but prey capture behaviors are less well characterized. High-speed digital imaging was used to record the axial kinematics of larval zebrafish as they preyed upon paramecia and also during other types of swimming. In all types of swim bouts, a series of traveling waves of bending is observed and these bends propagate along the trunk in the rostral to caudal direction. The prey capture swim bouts appeared to be more complex than other swim patterns examined. In the capture swim bouts, the initial bends were of low amplitude and were most prominent at far-caudal locations during each individual traveling wave of bending. Later bends in the bout (occurring just prior to prey capture) appeared to originate more rostrally and were of larger amplitude. These changes in bending pattern during capture swims were accompanied by a marked increase in tail-beat frequency. Associated with these axial kinematics were changes in heading and an abrupt increase in velocity close to the moment of prey capture. These changing patterns of bending suggest precise, bend-to-bend, neural control over both the timing and the rostral-caudal locus of bending. This degree of ‘fine axial motor control’ has not previously been described in the teleost behavioral literature and is notable because it occurs in larval zebrafish, where descending control signals are funneled through the roughly three-hundred neurons that project from brain into spinal cord. These findings will necessitate a significant increase in the complexity of current models of descending motor control in fishes.
Euglena gracilis growth and cell composition under different temperature, light and trophic conditions

Background Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds. The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids). Comparisons were primarily carried out in cultures grown at 23 °C, but the effect of growth at higher temperatures (27 or 30 °C) was also considered. Cell growth Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer. Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions. Temperature had less effect on cell composition. Cell composition Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon. These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected. The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated. The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically. Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells. α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark. Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass. Light was also important for the production of vitamin E and phytol.
Activity and Kinematics of Ultracool Dwarfs, Including an Amazing Flare Observation

We present the activity and kinematics of a representative volume-limited (20 pc) sample of 152 late-M and L dwarfs (M7-L8) photometrically selected from the Two Micron All Sky Survey (2MASS). Using new proper-motion measurements and spectrophotometric distance estimates, we calculate tangential velocities. The sample has a mean tangential velocity of ⟨Vtan⟩ = 31.5 km s-1, a velocity dispersion of σtan = 20.7 km s-1, and a maximum tangential velocity of Vtan = 138.8 km s-1. These kinematic results are in excellent agreement with previous studies of ultracool dwarfs in the local solar neighborhood. Hα emission, an indicator of chromospheric activity, was detected in 63 of 81 late-M dwarfs and in 16 of 69 L dwarfs examined. We find a lack of correlation between activity strength, measured by log(FHα/Fbol), and Vtan, although velocity distributions suggest that the active dwarfs in our sample are slightly younger than the inactive dwarfs. Consistent with previous studies of activity in ultracool dwarfs, we find that the fraction of Hα-emitting objects per spectral type peaks at spectral type M7 and declines through mid-L dwarfs. Activity strength is similarly correlated with spectral type for types later than M7. Eleven dwarfs out of 150 show evidence of variability, ranging from small fluctuations to large flare events. We estimate a flare cycle of ∼5% for late-M dwarfs and ∼2% for L dwarfs. Observations of strong, variable activity on the L1 dwarf 2MASS J10224821+5825453 and an amazing flare event on the M7 dwarf 2MASS J1028404-143843 are discussed.
1 Heterointerface effects in the electro-intercalation of van der Waals heterostructures

1 Heterointerface effects in the electro-intercalation of van der Waals heterostructures D. Kwabena Bediako, Mehdi Rezaee, Shu Yang Frank Zhao, Takashi Taniguchi, Kenji Watanabe, Tina L. Brower-Thomas and Philip Kim* 1 Department of Physics, Harvard University, Cambridge, Massachusetts 02138, USA 2 Department of Electrical Engineering, Howard University, Washington, DC 20059, USA 3 National Institute for Materials Science, Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan 4 Department of Chemical Engineering, Howard University, Washington, DC 20059, USA † These authors contributed equally to this work
Cooperative Interaction between Phosphorylation Sites on PERIOD Maintains Circadian Period in Drosophila

Circadian rhythms in Drosophila rely on cyclic regulation of the period (per) and timeless (tim) clock genes. The molecular cycle requires rhythmic phosphorylation of PER and TIM proteins, which is mediated by several kinases and phosphatases such as Protein Phosphatase-2A (PP2A) and Protein Phosphatase-1 (PP1). Here, we used mass spectrometry to identify 35 “phospho-occupied” serine/threonine residues within PER, 24 of which are specifically regulated by PP1/PP2A. We found that cell culture assays were not good predictors of protein function in flies and so we generated per transgenes carrying phosphorylation site mutations and tested for rescue of the per01 arrhythmic phenotype. Surprisingly, most transgenes restore wild type rhythms despite carrying mutations in several phosphorylation sites. One particular transgene, in which T610 and S613 are mutated to alanine, restores daily rhythmicity, but dramatically lengthens the period to ∼30 hrs. Interestingly, the single S613A mutation extends the period by 2–3 hours, while the single T610A mutation has a minimal effect, suggesting these phospho-residues cooperate to control period length. Conservation of S613 from flies to humans suggests that it possesses a critical clock function, and mutational analysis of residues surrounding T610/S613 implicates the entire region in determining circadian period. Biochemical and immunohistochemical data indicate defects in overall phosphorylation and altered timely degradation of PER carrying the double or single S613A mutation(s). The PER-T610A/S613A mutant also alters CLK phosphorylation and CLK-mediated output. Lastly, we show that a mutation at a previously identified site, S596, is largely epistatic to S613A, suggesting that S613 negatively regulates phosphorylation at S596. Together these data establish functional significance for a new domain of PER, demonstrate that cooperativity between phosphorylation sites maintains PER function, and support a model in which specific phosphorylated regions regulate others to control circadian period.
Multiple Category-Lot Quality Assurance Sampling: A New Classification System with Application to Schistosomiasis Control

Background Originally a binary classifier, Lot Quality Assurance Sampling (LQAS) has proven to be a useful tool for classification of the prevalence of Schistosoma mansoni into multiple categories (≤10%, >10 and <50%, ≥50%), and semi-curtailed sampling has been shown to effectively reduce the number of observations needed to reach a decision. To date the statistical underpinnings for Multiple Category-LQAS (MC-LQAS) have not received full treatment. We explore the analytical properties of MC-LQAS, and validate its use for the classification of S. mansoni prevalence in multiple settings in East Africa. Methodology We outline MC-LQAS design principles and formulae for operating characteristic curves. In addition, we derive the average sample number for MC-LQAS when utilizing semi-curtailed sampling and introduce curtailed sampling in this setting. We also assess the performance of MC-LQAS designs with maximum sample sizes of n = 15 and n = 25 via a weighted kappa-statistic using S. mansoni data collected in 388 schools from four studies in East Africa. Principle Findings Overall performance of MC-LQAS classification was high (kappa-statistic of 0.87). In three of the studies, the kappa-statistic for a design with n = 15 was greater than 0.75. In the fourth study, where these designs performed poorly (kappa-statistic less than 0.50), the majority of observations fell in regions where potential error is known to be high. Employment of semi-curtailed and curtailed sampling further reduced the sample size by as many as 0.5 and 3.5 observations per school, respectively, without increasing classification error. Conclusion/Significance This work provides the needed analytics to understand the properties of MC-LQAS for assessing the prevalance of S. mansoni and shows that in most settings a sample size of 15 children provides a reliable classification of schools.
Silencing of ZNF 139-siRNA induces apoptosis in human gastric cancer cell line BGC 823

Background and purpose: ZNF139, a member of zinc finger protein family, is a transcription factor. Our previous studies have showed that the over-expression of ZNF139 in gastric cancer (GC) cells was related to the differentiation of GC. However, the function of ZNF139 in GC cells’ apoptosis is still unclear. In present study, endogenous ZNF139 in GC cell line BGC823 was inhibited with siRNA, and then mechanism of ZNF139 in GC cells’ apoptosis was investigated. Methods: Expression of ZNF139 in GC tissues, adjacent normal tissues, GC cell lines MKN28, SGC7901, BGC823 and that in gastric epithelial cell line GES-1 were tested. Then ZNF139-specific siRNA was transfected into BGC823 cells. Viability, cell cycle and apoptosis of GC cells were detected. Survivin, x-IAP, caspase-3, Fas, p53, Bcl-2 and Bax genes were detected with QPCR and Western blot. Results: ZNF139 expression in GC tissues was significantly higher than that in adjacent normal tissues; ZNF139 expression in GES-1 was very weak, but it expressed in various GC cell lines, with the highest expression in BGC823. After endogenous ZNF139 was inhibited with ZNF139-siRNA, FCM indicated that after transfection, GC cells in G0/G1 phase was significantly increased, but was significantly reduced in G2/M phases; also after transfection, the apoptotic rate of BGC823 cells increased significantly. 48 h after ZNF139-siRNA was transfected, the expression of Survivin, x-IAP and Bcl-2 was significantly down-regulated, while the expression of caspase-3 and Bax was significantly up-regulated. Conclusion: Our results suggest that ZNF139 functions to promote apoptosis resistance of BGC823 by regulating some apoptosis related genes.
Does marriage protect against hospitalization with pneumonia? A population-based case-control study

Background To reduce the increasing burden of pneumonia hospitalizations, we need to understand their determinants. Being married may decrease the risk of severe infections, due to better social support and healthier lifestyle. Patients and methods In this population-based case-control study, we identified all adult patients with a first-time pneumonia-related hospitalization between 1994 and 2008 in Northern Denmark. For each case, ten sex- and age-matched population controls were selected from Denmark’s Civil Registration System. We performed conditional logistic regression analysis to estimate the odds ratios (ORs) for pneumonia hospitalization among persons who were divorced, widowed, or never married, as compared with married persons, adjusting for age, sex, 19 different comorbidities, alcoholism-related conditions, immunosuppressant use, urbanization, and living with small children. Results The study included 67,162 patients with a pneumonia-related hospitalization and 671,620 matched population controls. Compared with controls, the pneumonia patients were more likely to be divorced (10% versus 7%) or never married (13% versus 11%). Divorced and never-married patients were much more likely to have previous diagnoses of alcoholism-related conditions (18% and 11%, respectively) compared with married (3%) and widowed (6%) patients. The adjusted OR for pneumonia-related hospitalization was increased, at 1.29 (95% confidence interval [CI]: 1.25–1.33) among divorced; 1.15 (95% CI: 1.12–1.17) among widowed; and 1.33 (95% CI: 1.29–1.37) among never-married individuals as compared with those who were married. Conclusion Married individuals have a decreased risk of being hospitalized with pneumonia compared with never-married, divorced, and widowed patients.
Fucoxanthin and Polyunsaturated Fatty Acids Co-Extraction by a Green Process

By their autotrophic nature and their molecular richness, microalgae are serious assets in the context of current environmental and societal challenges. Some species produce both omega-3 long chain polyunsaturated fatty acids (PUFAs) and xanthophylls, two molecular families widely studied for their bioactivities in the fields of nutrition and cosmetics. Whereas most studies separately deal with the two families, synergies could be exploited with extracts containing both PUFAs and xanthophylls. The purpose of our work was to determine cost effective and eco-friendly parameters for their co-extraction. The effect of several parameters (solvent, solvent/biomass ratio, temperature, duration) were studied, using two microalgal species, the non-calcifying Haptophyta Tisochrysis lutea, and the diatom Phaeodactylum tricornutum, that presents a silicified frustule. Analyses of PUFAs and fucoxanthin (Fx), the main xanthophyll, allowed to compare kinetics and extraction yields between experimental protocols. Co-extraction yields achieved using 96% ethanol as solvent were 100% for Fx and docosahexaenoic acid (DHA) in one hour from T. lutea biomass, and respectively 95% and 89% for Fx and eicosapentaenoic acid (EPA) in eight hours from P. tricornutum. These conditions are compatible with industrial applications.
Could changing ocean circulation have destabilized methane hydrate at the Paleocene/Eocene boundary?

[1] During the Paleocene-Eocene Thermal Maximum (PETM, ∼55 Ma), marine and terrestrial carbon isotope values exhibit a negative shift of at least 2.5‰, indicative of massive destabilization of marine methane hydrates, releasing ∼2000 gigatons of methane carbon. The cause of the hydrate destabilization is unknown but has been speculated to be warming due to a change from high-latitude to low-latitude deepwater formation. Here we present results from a numerical ocean model indicating that a sudden switch of deepwater formation from southern to northern high latitudes caused middepth and deep-ocean warming of 3°–5°C. The switch is caused by a slow increase in the intensity of the atmospheric hydrologic cycle, as expected under increasing temperatures and consistent with PETM sedimentary evidence. Deepened subtropical subduction prior to the thermohaline circulation switch causes warming of 1°–4°C in limited areas at thermocline through upper intermediate depths, which could destabilize methane hydrates gradually and at progressively greater depths. Warming accompanying a south-to-north switch in deepwater formation would produce sufficient warming to destabilize seafloor gas hydrates over most of the world ocean to a water depth of at least 1900 m.
Interprofessional Rounds Improve Timing of Appropriate Palliative Care Consultation on a Hospitalist Service

Despite known benefits, palliative care (PC) consultation for hospitalized patients remains underutilized. The objective was to improve frequency and timeliness of appropriate inpatient PC consultation. On 2 of 11 hospitalist teams, a PC representative attended discharge rounds twice a week. Control teams’ discharge rounds were unenhanced. Subjects were all patients admitted to a hospitalist service in a quaternary academic medical center. The primary outcome was change in provision of PC consultation over time; the secondary outcome was change in time-to-consult (days). Hospitalists were surveyed regarding the intervention. The unadjusted proportion of patients receiving PC consultation increased from 2.7% to 5.2% on the intervention teams. Compared to control teams over time and adjusting for multiple covariates, the intervention increased PC consultation (difference-in-difference [DID] = 1.0 percentage-point increase [95% CI = 0.3%-1.8%]) and decreased time to consult (DID = −5 days [95% CI = −11 to −1]) in patients admitted for noncancer diagnoses. Hospitalists thought the intervention facilitated effective patient care without increased burden.
Disrupting Sleep: The Effects of Sleep Loss on Psychotic Experiences Tested in an Experimental Study With Mediation Analysis

Abstract Our view is that insomnia may be a causal factor in the occurrence of psychotic experiences such as paranoia and hallucinations. However, the causal relationship is not established. The aim of the study was to investigate the causal role of insomnia in psychotic experiences via a sleep restriction manipulation. The study was a within-subjects crossover design that included a planned mediation analysis. Sixty-eight nonclinical volunteers underwent a sleep loss condition (restricted to 4 h sleep for 3 nights) and a control condition (standard sleep) in randomized order in 2 consecutive weeks, with a weekend washout period. Psychotic experiences (paranoia, hallucinations, grandiosity, and cognitive disorganization) and candidate mediating variables (negative affect and related processes, working memory, decision making, and perceptual processing) were assessed before and after each condition. Actigraphy verified an average sleep duration of 5 h 15 min in the sleep loss condition, vs 6 h 58 min in the control condition. After the sleep loss condition, relative to the control condition, participants reported significant increases in paranoia, hallucinations, and cognitive disorganization, with no significant changes in grandiosity. The sleep loss condition was also associated with significant increases in negative affect, negative self and other cognitions, worry, and working memory impairment. Mediation analyses indicated that changes in psychotic experiences were mediated by changes in negative affect and related processes, but not memory impairment. The overall conclusion is that insomnia has a causal role in the occurrence of certain psychotic experiences, and that a key route is via negative affect.