Miracle-dz/UniFAR-FTUs huggingface dataset:
Stromal cell-derived factor-1 promoted angiogenesis and inflammatory cell infiltration in aneurysm walls.

OBJECT A small percentage of cerebral aneurysms rupture, but when they do, the effects are devastating. Current management of unruptured aneurysms consists of surgery, endovascular treatment, or watchful waiting. If the biology of how aneurysms grow and rupture were better known, a novel drug could be developed to prevent unruptured aneurysms from rupturing. Ruptured cerebral aneurysms are characterized by inflammation-mediated wall remodeling. The authors studied the role of stromal cell-derived factor-1 (SDF-1) in inflammation-mediated wall remodeling in cerebral aneurysms. METHODS Human aneurysms, murine carotid artery aneurysms, and murine intracranial aneurysms were studied using immunohistochemistry. Flow cytometry analysis was performed on blood from mice developing carotid or intracranial aneurysms. The effect of SDF-1 on endothelial cells and macrophages was studied by chemotaxis cell migration assay and capillary tube formation assay. Anti-SDF-1 blocking antibody was given to mice and compared with control (vehicle)-administered mice for its effects on the walls of carotid aneurysms and the development of intracranial aneurysms. RESULTS Human aneurysms, murine carotid aneurysms, and murine intracranial aneurysms all expressed SDF-1, and mice with developing carotid or intracranial aneurysms had increased progenitor cells expressing CXCR4, the receptor for SDF-1 (p < 0.01 and p < 0.001, respectively). Human aneurysms and murine carotid aneurysms had endothelial cells, macrophages, and capillaries in the walls of the aneurysms, and the presence of capillaries in the walls of human aneurysms was associated with the presence of macrophages (p = 0.01). Stromal cell-derived factor-1 promoted endothelial cell and macrophage migration (p < 0.01 for each), and promoted capillary tube formation (p < 0.001). When mice were given anti-SDF-1 blocking antibody, there was a significant reduction in endothelial cells (p < 0.05), capillaries (p < 0.05), and cell proliferation (p < 0.05) in the aneurysm wall. Mice given anti-SDF-1 blocking antibody developed significantly fewer intracranial aneurysms (33% vs 89% in mice given control immunoglobulin G, respectively; p < 0.05). CONCLUSIONS These data suggest SDF-1 is associated with angiogenesis and inflammatory cell migration and proliferation in the walls of aneurysms, and may have a role in the development of intracranial aneurysms.
Temporal overlaps of feral cats with prey and competitors in primary and human-altered habitats on Bohol Island, Philippines

The vertebrate fauna of the Philippines, known for its diversity and high proportion of endemic species, comprises mainly small- to medium-sized forms with a few large exceptions. As with other tropical ecosystems, the major threats to wildlife are habitat loss, hunting and invasive species, of which the feral cat (Felis catus) is considered the most damaging. Our camera-trapping study focused on a terrestrial vertebrate species inventory on Bohol Island and tempo-spatial co-occurrences of feral cats with their prey and competitors. The survey took place in the Rajah Sikatuna Protected Landscape, and we examined the primary rainforest, its border with agricultural land, and rural areas in the vicinity of villages. Altogether, over 2,885 trap days we captured 30 species of vertebrates–10 mammals (including Sus philippensis), 19 birds and one reptile, Varanus cumingi. We trapped 81.8% of expected vertebrates. Based on the number of events, the most frequent native species was the barred rail (Gallirallus torquatus). The highest overlap in diel activity between cats and potential prey was recorded with rodents in rural areas (Δ = 0.62); the lowest was in the same habitat with ground-dwelling birds (Δ = 0.40). Cat activity was not recorded inside the rainforest; in other habitats their diel activity pattern differed. The cats’ activity declined in daylight in the proximity of humans, while it peaked at the transition zone between rainforest and fields. Both rodents and ground-dwelling birds exhibited a shift in activity levels between sites where cats were present or absent. Rodents tend to become active by day in cat-free habitats. No cats’ temporal response to co-occurrences of civets (Paradoxurus hermaphroditus and Viverra tangalunga) was found but cats in diel activity avoided domestic dogs (Canis lupus familiaris). Our first insight into the ecology of this invasive predator in the Philippines revealed an avoidance of homogeneous primary rainforest and a tendency to forage close to human settlements in heterogeneous habitats. A detailed further investigation of the composition of the cat’s diet, as well as ranging pattern, is still needed.
Origin of CD57+ T cells which increase at tumour sites in patients with colorectal cancer

Human T cells carrying natural killer (NK) markers, CD57 or CD56 antigens, appear to be distinguishable from other T cell subsets in terms of their granular lymphocyte morphology and their numerical increase in patients with AIDS and in recipients of bone marrow transplantation. At the begining of this study, we observed that CD57+ T cells as well as CD56+ T cells were abundant at tumour sites in many patients with colorectal cancer. Since all these findings for CD57+ T cells are quite similar to those of extrathymic T cells seen in mice, we investigated how CD57+ T cells are distributed to various immune organs in humans. They were found to be present mainly in the bone marrow and liver, but to be completely absent in the thymus. Similar to the case of extrathymic T cells in mice, they were observed to consist of double‐negative CD48+ subsets as well as single‐positive subsets (preponderance of CD8+ cells), and to contain a considerable proportion of γδ T cells. These features are striking when compared with those of CD57+ T cells, which are characterized by an abundance of CD4+ subsets and αβ T cells. Not only at tumour sites but also in the peripheral blood, some patients with colorectal cancer displayed elevated levels of CD57+ cells. These results suggest that CD57+ T cells may be of extrathymic origin, possibly originating in the bone marrow and liver, and may be associated with tumour immunity, similar to another extrathymic population of CD56+ T cells in humans.
"I knew they clicked when i saw them with their friends": identifying your silent web visitors on social media

An increasing fraction of users access content on the web from social media. Endorsements by microbloggers and public figures you connect with gradually replaces the curation originally in the hand of traditional media sources. One expects a social media provider to possess a unique ability to analyze audience and trends since they collect not only information about what you actively share, but also about what you silently watch. Your behavior in the latter seems safe from observations outside your online service provider, for privacy but also commercial reasons. In this paper, we show that supposing that your passive web visits are anonymous to your host is a fragile assumption, or alternatively that third parties -- content publishers or providers serving ads onto them -- can efficiently reconciliate visitors with their social media identities. What is remarkable in this technique is that it need no support from the social media provider, it seamlessly applies to visitors who \emph{never} post or endorse content, and a visitor's public identity become known after a few clicks. This method combines properties of the public follower graph with posting behaviors and recent time-based inference, making it difficult to evade without drastic or time-wasting measures. It potentially offers researchers working on traffic datasets a new view into who access content or through which channels.
CspR, a Cold Shock RNA-Binding Protein Involved in the Long-Term Survival and the Virulence of Enterococcus faecalis

ABSTRACT By coprecipitation, we identified RNA-binding proteins in the Gram-positive opportunistic pathogen Enterococcus faecalis known to be deficient of the RNA chaperone Hfq. In particular, we characterized one belonging to the cold shock protein (Csp) family (Ef2925) renamed CspR for cold shock protein RNA binding. Compared to the wild-type strain, the ΔcspR mutant was less virulent in an insect infection model (Galleria mellonella) and exhibited a decreased persistence in mouse kidneys and a low survival rate in peritoneal macrophages. As expected, we found that the ΔcspR mutant strain was more impaired in its growth than the parental strain under cold conditions and in its long-term survival under nutrient starvation. All these phenotypes were restored after complementation of the ΔcspR mutant. In addition, Western blot analysis showed that CspR was overexpressed under cold shock conditions and in the stationary phase. Since CspR may act as an RNA chaperone, putative targets were identified using a global proteomic approach completed with transcriptomic assays. This study revealed that 19 proteins were differentially expressed in the ΔcspR strain (9 upregulated, 10 downregulated) and that CspR mainly acted at the posttranscriptional level. These data highlight for the first time the role of the RNA-binding protein CspR as a regulator in E. faecalis and its requirement in stress response and virulence in this important human pathogen.
From structure from motion to historical building information modeling: populating a semantic-aware library of architectural elements

Abstract. In recent years, we have witnessed a huge diffusion of building information modeling (BIM) approaches in the field of architectural design, although very little research has been undertaken to explore the value, criticalities, and advantages attributable to the application of these methodologies in the cultural heritage domain. Furthermore, the last developments in digital photogrammetry lead to the easy generation of reliable low-cost three-dimensional textured models that could be used in BIM platforms to create semantic-aware objects that could compose a specific library of historical architectural elements. In this case, the transfer between the point cloud and its corresponding parametric model is not so trivial and the level of geometrical abstraction could not be suitable with the scope of the BIM. The aim of this paper is to explore and retrace the milestone works on this crucial topic in order to identify the unsolved issues and to propose and test a unique and simple workflow practitioner centered and based on the use of the latest available solutions for point cloud managing into commercial BIM platforms.
The effect of carbamazepine on valproic acid disposition in adult volunteers.

1. Pharmacokinetic parameters for valproic acid (VPA) were determined before and following 2 weeks of carbamazepine (CBZ) administration in five healthy male volunteers. Mean VPA dosage was 16.4 mg kg-1 day-1. CBZ dosage was started at 100 mg twice daily and increased after 1 week to a total daily dose of 300 mg. 2. After CBZ administration, mean VPA plasma clearance increased from 0.90 +/- 0.18 s.d. to 1.26 +/- 0.24 l h-1 (P less than 0.05) as did clearance of free VPA (20.8 +/- 7.6 to 37.0 +/- 13.6 l h-1). Mean VPA elimination rate constant increased from 0.051 +/- 0.011 to 0.067 +/- 0.011 h-1 (P less than 0.05) after CBZ administration. 3. Mean area under the serum concentration vs time curve decreased from 675.0 +/- 130.5 to 475.7 +/- 75.7 mg l-1 h (P less than 0.05) after CBZ administration. Mean serum VPA half-life decreased from 14.0 +/- 2.4 to 10.6 +/- 1.4 h (P less than 0.05). Mean serum VPA trough concentrations decreased from 44.0 +/- 16.7 to 27.0 +/- 10.4 micrograms ml-1 (P less than 0.05). 4. A significant change was not observed in the mean VPA volume of distribution after CBZ coadministration suggesting that enzyme induction rather than a competition for plasma protein binding sites was involved in this interaction. 5. Despite the increased clearance of VPA, the urinary recovery of VPA or conjugate did not increase after CBZ administration.
Antibody to very late activation antigen 4 prevents antigen-induced bronchial hyperreactivity and cellular infiltration in the guinea pig airways

This report examines the effect of an anti-VLA-4 monoclonal antibody (mAb) HP1/2 on antigen-induced bronchial hyperreactivity to methacholine, and on eosinophil and T lymphocyte infiltration in the airways of guinea pigs sensitized and challenged by aerosolized ovalbumin and used 24 h thereafter. The intravenous administration of 2.5 mg/kg of HP1/2, but not of its isotype-matched mAb 1E6, 1 h before and 4 h after antigen inhalation, markedly inhibited the increased bronchopulmonary responses to intravenous methacholine, as well as airway eosinophilia in bronchoalveolar lavage (BAL) fluid and in bronchial tissue. HP1/2 also suppressed the antigen-induced infiltration of the bronchial wall by CD4+ and CD8+ T lymphocytes, identified by immunohistochemical technique using specific mAbs that recognize antigenic epitopes of guinea pig T cells. Treatment with HP1/2 also resulted in a significant increase in the number of blood eosinophils, suggesting that inhibition by anti-VLA-4 mAb of eosinophil recruitment to the alveolar compartment may partially account for their accumulation in the circulation. These findings indicate that eosinophil and lymphocyte adhesion and subsequent infiltration into the guinea pig airways that follow antigen challenge are mediated by VLA-4. Furthermore, concomitant inhibition of antigen-induced bronchial hyperreactivity and of cellular infiltration by anti-VLA-4 mAb suggests a relationship between airway inflammation and modifications in the bronchopulmonary function.
Effects of Alcohol Use and Other Aspects of Lifestyle on Blood Pressure Levels and Prevalence of Hypertension in a Working Population

The relationship between alcohol consumption and blood pressure was studied in 491 males, ages 20–45 years, who volunteered to complete a health questionnaire and submit to standardized measurements of blood pressure, heart rate and body size. Average weekly alcohol consumption correlated with systolic pressure (r = 0.18) but not diastolic pressure. Systolic pressure increased progressively with increasing alcohol consumption, with no obvious threshold effect. In moderate and heavy drinkers (53% of the population studied), the prevalence of systolic hypertension (≥ 140 mm Hg) was four times that of teetotalers. The effect of alcohol on systolic blood pressure was independent of the effects of age, obesity, cigarette smoking and physical activity. Ex-heavy drinkers had blood pressures similar to those of teetotalers, suggesting that the effect of alcohol is reversible. Cigarette smokers had lower diastolic pressures than nonsmokers, an effect independent of obesity. The linear correlation between alcohol consumption and systolic blood pressure and the lower blood pressures in exdrinkers suggest a cause-and-effect relationship. The results indicate that alcohol ranks close to obesity as a potentially preventable cause of hypertension in the community.
Watching watts to prevent abuse of power

To successfully monitor power market operations and anticipate how market changes could affect the exercise of market power, indices of market power that are grounded in theory, reliable and easily calculated are needed. In this article, we present an index that we have found particularly useful in California: the Residual Supply Index (RSI). We demonstrate the empirical relationship between RSI and the mark-up of prices above competitive levels. We also describe two applications of this new index: using RSI to assess the overall competitiveness of the market during three years of dramatic changes in underlying structure, and estimating the benefits of increasing market competitiveness by upgrading Path 15. We also present some questions that market designers must consider when deciding how to mitigate market power when it is detected.
Complement anaphylatoxin C5a stimulates release of SRS‐A‐like activity from guinea‐pig lung fragments

A belief that anaphylatoxins are major agents in hypersensitivity reactions is as old as that relating to histamine. Studies on complement during the past eight years have elucidated the biochemistry and functional dynamics of the anaphylatoxin molecules, C3a and C5a. Previous studies concerning the pharmacological responses of tissues to anaphylatoxins usually employed impure material in tests which were heavily biased towards showing the effects of histamine. Thus anaphylatoxins have come to be regarded as releasers to tissue histamine which in turn was believed to cause the various observed responses. Evidence for the ability of anaphylatoxins to release histamine is compelling and has diverted attention from their possible role in mediating other indirect effects or of eliciting direct responses of susceptible cells. We recently reported (Stimler et al 1980) that peripheral strips of guinea pig lung underwent a very strong contraction in the presence of C5a at 1 pg ml-', a response that was not blocked by high concentrations of histamine antagonists. We have since found that when guinea-pig lung is perfused free of blood, chopped into fragments about 0.2 mm thick and incubated for lominutes in Tyrode solution at 37 "C, the addition of 1 pg ml-l of purified C5a induces the release of little histamine (15-20% of total; although significant amounts of a substance which appears to be a leukotriene are found. Furthermore, we find that contraction of the guinea-pig gut, previously rendered tachyphylactic to C5a, has a profile characteristic of SRS-A, and is blocked by FPL 55712, an inhibitor of SRS-A (Augstein et a1 1973). The quantities of SRS-A-like activity present in the lung supernatant are not large (120-130 U g-' lung), but it is likely that much of the activity is taken up by the tissue after being synthesized. We do not yet have either authentic standards or specific assays for leuko. trienes (eg R.I.A.) so that identity of the active substance remains somewhat tentative. Furthermore, the release of mediators in addition to histamine does not exclude the possibility that C5a also acts directly on the tissues. Our findings indicate a much wider role for complement in lung pathophysiology than has previously been envisaged. C5a will be generated via both the classical (antibody) and alternative (tissue enzyme) routes of complement activation and will therefore be present in conditions such as farmer's lung and bronchitis. The present work suggests that the final mediator substances histamine and SRS-A, which are known to produce bronchoconstriction in asthma, may also contribute to the dyspnoea in lung diseases which involve activation of complement. September 30, 1980
Can Children Teach their Parents about Asthma?

The Open Airways for Schools (OAS) program has been shown to improve the self-management skills and health outcomes of students with asthma in Grades 3 to 5. This report examines the impact of OAS on students’ parents. Because pilot studies showed that parental attendance at school-based sessions was low, the authors held six sessions at school for children and gave children homework assignments to complete with parents at home to teach parents about asthma and build support for children’s self-management efforts. Analysis of 1-year follow-up data showed that children’s participation in OAS was a significant predictor of parental self-management skills (p < .03) and that OAS children’s communication was more strongly associated than controls’ with parents’ self-management (p = .05). The findings show that health education activities brought home from school by children can positively influence parents’ self-management of a complex chronic disease such as asthma.
Effect of environment on insulin‐like growth factor I refolding selectivity

Recombinant human insulin‐like growth factor I (IGF‐I), a 70‐amino‐acid peptide containing three disulphide bonds, produces two monomeric and several multimeric species during refolding. To optimize production of correctly folded IGF‐I, conditions which influence protein refolding, stability and solubility were systematically examined. Combinations of solution components and conditions were analysed to identify synergistic interactions which enhance or reduce refolding efficiency. IGF‐I concentration had the largest effect on formation of correctly folded peptide, due to competing association reactions. Solution polarity had the next largest individual effect, both on the level of multimeric peptide and on the relative proportion of correctly folded to misfolded monomeric peptide. Salt type and concentration and chaotrope type and concentration also had large individual effects on the distribution of IGF‐I forms produced. Solution polarity modulated the effects of many other conditions including chaotrope concentration, salt type and concentration, and osmolyte concentration. Simultaneously decreasing the solution polarity and increasing the salt concentration improved the yield of correctly folded IGF‐I relative to either individual change. Optimum solution conditions for refolding were 2M urea, 1M NaCl and 20% (v/v) ethanol. A possible mechanism for the importance of solution polarity on IGF‐I refolding is discussed.
Crescentic Proximal Metatarsal Osteotomy for Moderate to Severe Hallux Valgus: A Mean 12.2 Year Follow-Up Study

The senior author's (R.C.) first 25 patients (37 feet) treated with a combination proximal crescentic osteotomy and distal soft-tissue reconstruction made up the patient cohort. All 25 patients were reviewed at a minimum of one year post-op (short-term follow-up) and 20/25 (31/37 feet) were reviewed again at a mean 12.2 years (range 11.4 to 13.0 yrs) post-op (long-term follow-up). This allowed for a comparison of short- and long-term results and led to a long-term follow-up rate of 84% (31/37 feet, mean 12.2 yrs). Clinical, radiographic and patient outcome measures were obtained and compared pre-op and at short- and long-term follow-up. The mean preoperative hallux valgus (HVA) and intermetatarsal angles (IMA) were 37° and 16° respectively. The mean HVA correction was 24° and IMA correction 10° at long-term follow-up with no tendency toward recurrence. Sesamoid position and first MTP subluxation was markedly improved postoperatively and the correction was maintained at long-term follow-up. Patients were asked about their satisfaction in terms of pain, appearance and motion. At long-term follow-up, more than 90% of patients were completely satisfied with pain and motion and greater than 80% with their appearance. Ninety-four percent of patients said they would have the operation again. The AOFAS clinical rating scale for the hallux was calculated retrospectively for pre-op and short-term follow-up and prospectively for long-term follow-up. The mean pre-op score was 37/100 (16 to 60) which significantly improved to 92/100 (67 to 100) at both follow-up periods, suggesting no evidence of decrease in outcome over time. Complications included two patients (5%) that were over-corrected into varus (one symptomatic, one asymptomatic), and four patients (11%) that were undercorrected, developing asymptomatic recurrences (>10° increase HVA) at long-term follow-up. In addition, two patients (5%) developed new transfer lesions postoperatively, likely related to technical error (one varus overcorrection, one dorsiflexion malunion). In conclusion, the long-term results, with a mean follow-up of 12.2 years, of the resection realignment procedure for moderate to severe hallux valgus are generally excellent and the complication rate is low and acceptable. Attention to detail, avoiding both undercorrection, which can lead to recurrence, and overcorrection, which can cause symptomatic varus, is essential.