Miracle-dz/UniFAR-FTUs huggingface dataset:
Ah receptor in human placenta: stabilization by molybdate and characterization of binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin, 3-methylcholanthrene, and benzo(a)pyrene.

Aryl hydrocarbon hydroxylase (AHH, cytochrome P1-450) is highly inducible in several human cells and tissues exposed to specific halogenated and nonhalogenated aromatic chemicals of the "3-methylcholanthrene-type. " In laboratory animals AHH induction is known to be regulated by binding of inducers to the Ah receptor, a soluble intracellular protein. However, the induction mechanism in the human species is incompletely understood largely because the Ah receptor, which seems to be essential to the induction process, has not previously been detectable in certain human cells and tissues (including placenta) that are highly responsive to AHH induction. We found that human placenta contains high concentrations of Ah receptor (comparable to the receptor concentrations in rat and mouse liver) but that special modifications were necessary in the assay techniques in order to detect and accurately quantitate receptor binding. Receptor was detected at concentrations approximately equal to 100 fmol/mg cytosol protein using [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as the radioligand. This high concentration of specific binding sites was present only if the placental tissue was initially homogenized in a buffer containing sodium molybdate (10 or 20 mM). Without molybdate in the homogenizing buffer, specific [3H]TCDD binding was only about 35 fmol/mg. Specific Ah receptor binding also was detectable with [3H]-3-methylcholanthrene and, to a lesser extent, with [3H]-benzo(alpha)pyrene. The receptor sedimented near 9S on sucrose gradients whether molybdate was present or not. About 80% of specific binding was lost if excessive charcoal was used to adsorb "nonspecifically bound" ligand from cytosol prior to gradient analyses. The apparent affinity with which [3H]TCDD bound to Ah receptor in human placental cytosol was relatively low (apparent Kd approximately equal to 5 to 8 nM) when compared with the affinity of [3H]TCDD binding in rat or mouse hepatic cytosols (Kd approximately equal to 1 to 3 nM). These data suggest that while molybdate has very little effect on the quantity or molecular size of the rodent Ah receptor assay, it is very important in stabilizing the human Ah receptor. Our experiments demonstrate that human placenta contains a high concentration of Ah receptor and suggest that AHH induction in placenta is mediated through a receptor mechanism analogous to that previously established in tissues and cells from laboratory animals.
The Fanconi Anemia Pathway of Genomic Maintenance

Fanconi anemia (FA), a recessive syndrome with both autosomal and X-linked inheritance, features diverse clinical symptoms, such as progressive bone marrow failure, hypersensitivity to DNA cross-linking agents, chromosomal instability and susceptibility to cancer. At least 12 genetic subtypes have been described (FA-A, B, C, D1, D2, E, F, G, I, J, L, M) and all except FA-I have been linked to a distinct gene. Most FA proteins form a complex that activates the FANCD2 protein via monoubiquitination, while FANCJ and FANCD1/BRCA2 function downstream of this step. The FA proteins typically lack functional domains, except for FANCJ/BRIP1 and FANCM, which are DNA helicases, and FANCL, which is probably an E3 ubiquitin conjugating enzyme. Based on the hypersensitivity to cross-linking agents, the FA proteins are thought to function in the repair of DNA interstrand cross-links, which block the progression of DNA replication forks. Here we present a hypothetical model, which not only describes the assembly of the FA pathway, but also positions this pathway in the broader context of DNA cross-link repair. Finally, the possible role for the FA pathway, in particular FANCF and FANCB, in the origin of sporadic cancer is discussed.
Ammonium tetrathiomolybdate following ischemia/reperfusion injury: Chemistry, pharmacology, and impact of a new class of sulfide donor in preclinical injury models

Background Early revascularization of ischemic organs is key to improving outcomes, yet consequent reperfusion injury may be harmful. Reperfusion injury is largely attributed to excess mitochondrial production of reactive oxygen species (ROS). Sulfide inhibits mitochondria and reduces ROS production. Ammonium tetrathiomolybdate (ATTM), a copper chelator, releases sulfide in a controlled and novel manner, and may offer potential therapeutic utility. Methods and findings In vitro, ATTM releases sulfide in a time-, pH-, temperature-, and thiol-dependent manner. Controlled sulfide release from ATTM reduces metabolism (measured as oxygen consumption) both in vivo in awake rats and ex vivo in skeletal muscle tissue, with a superior safety profile compared to standard sulfide generators. Given intravenously at reperfusion/resuscitation to rats, ATTM significantly reduced infarct size following either myocardial or cerebral ischemia, and conferred survival benefit following severe hemorrhage. Mechanistic studies (in vitro anoxia/reoxygenation) demonstrated a mitochondrial site of action (decreased MitoSOX fluorescence), where the majority of damaging ROS is produced. Conclusions The inorganic thiometallate ATTM represents a new class of sulfide-releasing drugs. Our findings provide impetus for further investigation of this compound as a novel adjunct therapy for reperfusion injury.
Who Ate Whom? Adaptive Helicobacter Genomic Changes That Accompanied a Host Jump from Early Humans to Large Felines

Helicobacter pylori infection of humans is so old that its population genetic structure reflects that of ancient human migrations. A closely related species, Helicobacter acinonychis, is specific for large felines, including cheetahs, lions, and tigers, whereas hosts more closely related to humans harbor more distantly related Helicobacter species. This observation suggests a jump between host species. But who ate whom and when did it happen? In order to resolve this question, we determined the genomic sequence of H. acinonychis strain Sheeba and compared it to genomes from H. pylori. The conserved core genes between the genomes are so similar that the host jump probably occurred within the last 200,000 (range 50,000–400,000) years. However, the Sheeba genome also possesses unique features that indicate the direction of the host jump, namely from early humans to cats. Sheeba possesses an unusually large number of highly fragmented genes, many encoding outer membrane proteins, which may have been destroyed in order to bypass deleterious responses from the feline host immune system. In addition, the few Sheeba-specific genes that were found include a cluster of genes encoding sialylation of the bacterial cell surface carbohydrates, which were imported by horizontal genetic exchange and might also help to evade host immune defenses. These results provide a genomic basis for elucidating molecular events that allow bacteria to adapt to novel animal hosts.
Direct Anti-Inflammatory Mechanisms Contribute to Attenuation of Experimental Allograft Arteriosclerosis by Statins

Background—Despite the development of effective immunosuppressive therapy, transplant graft arterial disease (GAD) remains the major limitation to long-term graft survival. The interplay between host inflammatory cells and donor vascular wall cells results in an intimal hyperplastic lesion, which leads to ischemia and graft failure. HMG-CoA reductase inhibitors (statins) reduce GAD in human cardiac allografts, although it is unclear whether this is secondary to cholesterol lowering or other mechanisms. This study tested the hypothesis that statins can suppress GAD by cholesterol-independent pathways. Methods and Results—We performed heterotopic murine cardiac transplants in total allogeneic or major histocompatibility complex class II-mismatched combinations. Transplanted animals received either control chow, chow containing 25 ppm cerivastatin (low dose), or chow containing 125 ppm cerivastatin (high dose). Mean plasma cerivastatin concentrations were 0.0 (control), 10.1 (low dose), and 21.9 (high dose) nmol/L, respectively. Plasma cholesterol levels were the same in all groups. GAD scores decreased in low-dose (P <0.05) and high-dose (P <0.0001) cerivastatin groups compared with controls, with concomitant reduction in graft-infiltrating cells and significantly decreased intragraft RANTES and monocyte chemotactic protein-1 mRNA expression. Cerivastatin, as well as other statins, also reduced RANTES and monocyte chemotactic protein-1 production in mouse endothelial cells stimulated with interferon-&ggr; and tumor necrosis factor-&agr; in vitro. Conclusions—Clinically achievable levels of an HMG-CoA reductase inhibitor attenuate GAD in murine heart transplants, diminish host inflammatory cell recruitment, and do not alter cholesterol levels. These results indicate that statins can affect arterial biology and inflammation independently of their effects on cholesterol metabolism.
Influence of wood machining on tensile shear strength and wood failure percentage of one-component polyurethane bonded wooden joints after wetting

This study determines the influence of mechanical surfacing on tensile shear strength (TSS) and wood failure percentage (WFP) of beech wood (Fagus sylvatica L.) at the wet stage according to prEN 302-1:2011 (tensile shear tests). The wood was planed, sanded and face milled, using different qualities of cutting edges and sanding grits. Roughness and wettability of the adherends were characterised, supplemented by environmental scanning electron microscopy images. The specimens were bonded by means of a one-component polyurethane adhesive and tested at the dry stage (pretreatment A1), at the wet stage (A4) and after re-drying (mA5). Results determined that the wettability and the wood structure's integrity are considered more decisive for the TSS at the wet stage than surface roughness. Planing with very dull knives caused the lowest TSS and WFP after A4 and the most subsurface damage. Face milled and sanded batches revealed the best performance. The TSS and WFP decreased significantly from A1 to A4, but recovered after mA5.
A Study of the Prevalence of Self-Medication Practice among University Students in Southwestern Nigeria

Purpose: To estimate the prevalence of self-medication with antibiotics and antimalarials among university students in southwestern Nigeria and evaluate the factors associated with self-medication. Methods: A pre-tested questionnaire was used to collect data from 2000 university students using a convenient sampling technique. Prevalence of the practice of self-medication was estimated in percentages while factors associated with self-medication were evaluated using multiple regression analysis. Results: The prevalence of the practice of self-medication was high among the age group of 25 – 44 years but lower in the 15 - 24 and ≥ 45 year age groups, respectively. Females exhibited higher prevalence of self-medication than males. Among undergraduates, self-medication increased as the students’ class level in the university increased. Postgraduate students exhibited low prevalence of selfmedication practices. Self-medication was significantly associated with age, gender and students’ class level in the university at p` < 0.001. A majority, 982 (53.8 %), of the students used antibiotics for selfmedication while 845 (46.3 %) used anti-malarial drugs for self-medication. Sources of drugs for selfmedication were patent medicines store (901 or 49.3 %), community pharmacies (531 or 29.1 %), friends (210 or 11.5 %), relatives (130 or 7.1 %) and left-over drugs from previous prescriptions (55 or 3.0 %). Conclusion: The study revealed that age, gender and students’ level in the university influenced selfmedication practices. The use of antibiotics in self-medication calls for urgent health policy intervention. Keywords: Self-medication, Antimalarial, Antibiotics, University students, Nigeria.
Variable molecular clocks in hominoids

Generation time is an important determinant of a neutral molecular clock. There are several human-specific life history traits that led to a substantially longer generation time in humans than in other hominoids. Indeed, a long generation time is considered an important trait that distinguishes humans from their closest relatives. Therefore, humans may exhibit a significantly slower molecular clock as compared to other hominoids. To investigate this hypothesis, we performed a large-scale analysis of lineage-specific rates of single-nucleotide substitutions among hominoids. We found that humans indeed exhibit a significant slowdown of molecular evolution compared to chimpanzees and other hominoids. However, the amount of fixed differences between humans and chimpanzees appears extremely small, suggesting a very recent evolution of human-specific life history traits. Notably, chimpanzees also exhibit a slower rate of molecular evolution compared to gorillas and orangutans in the regions analyzed.
Effect of Scandium Addition on Mechanical Properties and Corrosion Resistance of Medium Strength Al-Zn-Mg-Cu) Alloy

Al-Zn-Mg alloys are widely used construct material for wide range of application including automotive industry, building, and aviation. Scandium is rare earth metal, which modifies structure of semi-products and can lead to increasing of tensile and yield strength because of Al3Sc intermetallics of nanoscale size. This paper describes results of influence of small addition of scandium (0,08 wt. %) on mechanical and corrosion properties of cold-rolled sheets of 1913 alloy. 1913 is Russian medium strength corrosion resistant weldable Al-Zn-Mg alloy with addition of Cu for improvement of stress corrosion resistance and Mn with Zr for structure improvement. It is shown that addition of Sc transforms kinetics of artificial aging and accelerates decomposition of solid solution, besides tensile strength increases up to 50 MPa in artificially aged tempers. Changes in microstructure and quench sensitivity are described; results of intergranural corrosion, exfoliation and stress corrosion tests are introduced after different heat treatment including peak strength aging and overaging tempers.
An efficient wrapper scan chain configuration method for network-on-chip testing

Network-on-chip (NoC) is the new paradigm for core-based system design. Reuse of the on-chip communication network for testing cores in a NoC-based system is critical to reduce test cost for this new architecture. However, many new challenging issues come up correspondingly. In this paper, we propose a test data transportation method with multiple data flit formats and a novel scan chain configuration method to maximize the utilization of the on-chip network channel without adding too much hardware overhead. Experimental results on ITC'02 benchmarks show that the new wrapper scan chain configuration method (with the aid of multiple data flit formats) leads to substantial reduction in network channel waste, and thus results in a significant overall reduction of time and energy for testing the entire system. The test wrapper architecture that supports the new method of test data transportation is very simple, and has been verified by VHDL simulation
Ontogeny and Oncogenesis Balance the Transcriptional Profile of Renal Cell Cancer

Global transcript analysis is increasingly used to describe cancer taxonomies beyond the microscopic reach of the eye. Diagnostic and prognostic portraits are formulated by ranking cancers according to transcriptional proximity. However, the role that distinct biological factors play in defining these portraits remains undefined. It is likely that the transcriptional repertoire of cancers depends, on one hand, on the anamnestic retention of their ontogenesis and, on the other, on the emergence of novel expression patterns related to oncogenesis. We compared the transcriptional profile of primary renal cell cancers (RCCs) with that of normal kidney tissue and several epithelial cancers of nonrenal origin to weigh the contribution that ontogeny and oncogenesis make in molding their genetic profile. Unsupervised global transcript analysis demonstrated that RCCs retain transcriptional signatures related to their ontogeny and cluster close to normal renal epithelium. When renal lineage-associated genes are removed from the analysis and cancer-specific genes are analyzed, RCCs segregate with other cancers with limited lineage specificity underlying a predominance of the oncogenic process over lineage specificity. However, a RCC-specific set of oncogenesis-related genes was identified and surprisingly shared by sarcomas. In summary, the transcriptional portrait of primary RCCs is largely dominated by ontogeny. Genes responsible for lineage specificity may represent poor molecular targets for immune or drug therapy. Most genes associated with oncogenesis are shared with other cancers and may represent better therapeutic targets. Finally, a small subset of genes is associated with lineage-specific oncogenesis, and these may provide information regarding the biological behavior of RCCs and facilitate diagnostic classification of RCCs.
A methodological proposal for monitoring, analyzing and Estimating Power Quality indices: the case of Bogotá-Colombia

The estimation of power quality (PQ) indices has a vital importance on the development of national PQ standards not only because provides truthful information about PQ conditions in our facilities, but also because permits to establish, with an own criterion, the values for the limits of the most common PQ disturbances. This paper proposes a methodology for the estimation of PQ indices based on statistical sampling and weighting factors techniques which are applied for commercial and industrial customers in the city of Bogota. In addition, some strategies for the classification of the type of customer as well as some final obtained values of PQ indices in Bogota are presented.
SOLUBILIZING EFFECT OF NUCLEOTIDES ON ISOLATED INSULIN SECRETORY GRANULES

ATP has been reported to cause release of hormone from secretory granules isolated from the adrenal medulla (Poisner and Trifaro, 1967) and the posterior pituitary (Poisner and Douglas, 1968) . This effect was suggested to be linked to enzymatic cleavage of the nucleotide, both the hormone release and the ATPase activity being inhibited by AMP. Using a partially purified fraction of insulin secretory granules from obese-hyperglycemic mice, we observed that ATP also has a solubilizing action on granule-bound insulin (Coore et al ., 1969) . The present study was undertaken to test whether the sensitivity of the /3-granules to ATP can be explained by the same mechanisms that have been proposed for the other endocrine granules . A number of nucleotides were investigated for effects on the stability of isolated /3-granules . In addition, the presence of ATP-splitting activity in this /3-cell fraction was checked with regard to its sensitivity to AMP.
Agreement of urine specific gravity measurements between manual and digital refractometers.

CONTEXT Urine specific gravity (Usg), measured by a handheld manual refractometer (MAN), has been recognized as a valid and practical means of assessing hydration status. Newer, digital refractometers are faster and more user friendly but have not been validated against the traditional MAN. OBJECTIVE To compare the reliability and validity of 2 digital refractometer models and a MAN. DESIGN Descriptive laboratory study. SETTING Research laboratory. PATIENTS OR OTHER PARTICIPANTS Sample of convenience was recruited from the local university and surrounding community (n = 82). INTERVENTION(S) Participants provided multiple urine samples (n = 124) over a 5-month period under various hydration conditions. MAIN OUTCOME MEASURE(S) Urine specific gravity was compared among a MAN, a digital refractometer requiring the prism to be dipped (DIP) into a urine sample, and a digital refractometer that requires urine to be pipetted (PIP) onto its prism for analysis. RESULTS The MAN measurements were strongly correlated with the DIP (r = 0.99, P < .001) and PIP (r = 0.97, P < .001) measurements. Bland-Altman analyses revealed slight mean underestimation (95% upper and lower levels of agreement) between MAN and DIP (-0.0012 [0.0028] and PIP -0.0011 [0.0035], respectively) and trends toward increased underestimation at higher Usg. Measurement error ≥ . 005 was greater for PIP (4/124, 3.2%) than for DIP (2/124, 1.6%). CONCLUSIONS Negligible differences were exhibited between PIP and DIP, with both displaying acceptable reliability and validity compared with the MAN. However, the Bland-Altman analysis suggests underestimation bias for the DIP and PIP as Usg increases, with the potential for rare but substantial underestimation when using PIP that should be recognized by clinicians, particularly when used as a screening measure in weight-class sports.
Using Pharmacologic Data to Plan Clinical Treatments for Patients with Peritoneal Surface Malignancy

The surfaces of the abdomen and pelvis are an important anatomic site for the dissemination of gastrointestinal and gynecologic malignancy. This transcoelomic spread of cancer cells gives rise to peritoneal carcinomatosis which, without special treatments, is a fatal manifestation of these diseases. In order to control peritoneal carcinomatosis cytoreductive surgery to remove gross disease is combined with perioperative intraperitoneal and perioperative intravenous chemotherapy to eradicate microscopic residual disease. Chemotherapy agents are selected to be administered by the intraperitoneal or intravenous route based on their pharmacologic properties. A peritoneal-plasma barrier which retards the clearance of high molecular weight chemotherapy from the peritoneal cavity results in a large exposure of small cancer nodules on abdominal and pelvic surfaces. Tissue penetration is facilitated by moderate hyperthermia (41-42ºC) of the intraperitoneal chemotherapy solution. A constant dose of chemotherapy agent and volume of carrier solution based on body surface area allows prediction of systemic drug exposure and systemic toxicity. Timing of the chemotherapy as a planned part of the surgical procedure to maximize exposure of all peritoneal surfaces is crucial to success.