Miracle-dz/UniFAR-FTUs huggingface dataset:
GH3 Cell-specific Expression of Kv1.5 Gene

A silencer element (Kv1.5 repressor element; KRE) was characterized by deletion analyses in the promoter of Kv1.5, a voltage-gated potassium channel. The silencer element selectively decreases expression of Kv1.5- and thymidine kinase-chloramphenicol acetyltransferase reporter gene constructs in cell lines that do not express Kv1.5 polypeptide. It contains a dinucleotide repetitive element (poly(GT)(GA)(CA)(GA)), and self-associates spontaneously in vitro to form complexes with slow electrophoretic mobility. Deletion of the repetitive element abolished self-association in vitro and the silencing activity in transient transfection experiments in vivo. Electromobility gel shift assays of KRE with GH3 cells nuclear extracts detected the formation of a unique DNA-protein complex, which was not detectable in Chinese hamster ovary and COS-7 cells. This complex does not react with an antibody against nonhistone high mobility group 1 protein, which binds KRE in gel retardation assays. These observations establish that a dinucleotide tandem repeat sequence, capable of self-association, forms part of a cell-specific silencer element in a mammalian gene.
A Computational Study of the Hydrodynamics of Gas-Solid Fluidized Beds

Computational fluid dynamics (CFD) modeling was used to predict the gas-solid hydrodynamics of fluidized beds. An Eulerian-Eulerian multi-fluid model and granular kinetic theory were used to simulate fluidization and to capture the complex physics associated therewith. The commercial code ANSYS FLUENT was used to study two-dimensional single solids phase glass bead and walnut shell fluidized beds. Current modeling codes only allow for modeling of spherical, uniform-density particles. Owing to the fact that biomass material, such as walnut shell, is abnormally shaped and has non-uniform density, a study was conducted to find the best modeling approach to accurately predict pressure drop, minimum fluidization velocity, and void fraction in the bed. Furthermore, experiments have revealed that all of the bed mass does not completely fluidize due to agglomeration of material between jets in the distributor plate. It was shown that the best modeling approach to capture the physics of the biomass bed was by correcting the amount of mass present in the bed in order to match how much material truly fluidizes experimentally, whereby the initial bed height of the system is altered. The approach was referred to as the SIM approach. A flow regime identification study was also performed on a glass bead fluidized bed to show the distinction between bubbling, slugging, and turbulent flow regimes by examining void fraction contours and bubble dynamics, as well as by comparison of simulated data with an established trend of standard deviation of pressure versus inlet gas velocity. Modeling was carried out with and without turbulence modeling ( ), to show the effect of turbulence modeling on two-dimensional simulations.
Nimodipine Pharmacotherapeutic Adjuvant Therapy for Inpatient Treatment of Cocaine Dependence

Summary:Recent preclinical studies suggest utility for voltage-sensitive calcium channel blockers (VSCCBs) in the treatment of cocaine addiction. The following double-blind placebo-controlled study examined the role of the VSCCB nimodipine in attenuating cocaine craving in 66 recently abstinent cocaine-dependent patients on an inpatient substance abuse treatment unit utilizing an intensive 12-step milieu-oriented psychosocial therapy. While the medication was well tolerated, the dose of nimodipine used in this study (90 mg q.d.) was not superior to placebo in reducing background or cue-induced cocaine craving over the 3 weeks of the study. There was the suggestion that nimodipine might attenuate the severity of some cocaine-induced brain deficits, as detected by evaluation of smooth pursuit eye movement function. A rationale for evaluating higher doses of nimodipine for the treatment of cocaine addiction is presented. As nimodipine might have anticraving and moodstabilizing properties and cardio- and neuroprotective properties in the face of cocaine intoxication and might possibly even reverse some cocaine-induced brain deficits, further investigation of the role of nimodipine (and other VSCCBs) in cocaine addiction appears an attractive avenue of future medication development.
What Works in Falls Prevention After Stroke?: A Systematic Review and Meta-Analysis

Background and Purpose— Falls are common after stroke. Despite evidence that single and multifactorial interventions can reduce falls in older people, this issue remains relatively underexplored in stroke survivors. Effective fall prevention in this population has the potential to prevent injury, improve quality of life, and decrease the likelihood of subsequent fear of falling and activity restriction. The aim of this article was to review and integrate the research evidence relating to interventions that reduce falls after stroke. Methods— Published studies evaluating interventions to reduce falls in stroke survivors were retrieved and screened according to predetermined criteria. Included studies were independently assessed. Quality of trials was assessed using the Physiotherapy Evidence Database score. Pooling of results was undertaken for similar interventions with comparable outcomes using the inverse variance method. Results— Thirteen studies met the inclusion criteria, with pooling of results possible for only 2 types of intervention. Methodological quality of the included studies was variable with the main bias because of lack of blinding of participants and those administering the intervention. Variability in falls data reporting was seen across the studies. The only intervention shown to be effective in reducing falls was vitamin D for female stroke survivors in an institutional setting. Other interventions were no more effective than usual care. Conclusions— Fall risk is high in stroke survivors; however, the only intervention shown to be effective in reducing falls in this review was vitamin D supplementation. Consistency in outcome measurement would enable comparisons across studies. Additionally, further research evaluating a range of single and multifactorial interventions for fall prevention in the stroke population is required.
SHEAR STRENGTH OF REINFORCED CONCRETE WALLS WITH BOUNDARY MEMBER

The reinforced concrete wall is frequently used in the reinforced concrete structures as seismic resistant element. This is because its effectiveness in resisting lateral shear force due to earthquake. The reinforced concrete walls are usually with columns on both sides as the boundary members and it is known as the walls of barbell shape section. The shear strength of reinforced concrete walls with boundary member, as reported in the previous literature, is larger than the shear strength of reinforced concrete walls without boundary member. The larger strength is due to the resistance of boundary members to the end conditions of wall diagonal strut. The objective of this study is to verify the influence of boundary member in reinforced concrete walls by comparing the predicted shear strength of both reinforced concrete walls with and without boundary members effect. The prediction of shear strength of reinforced concrete walls that are considered as discontinuity region uses the Softened Strut-and-Tie Model. The model is based on the condition of fully cracked wall with the diagonal compression failure. Comparison of the predicted shear strength of some specimens that available in the literature was agree that the walls with boundary member effect indicated a shear strength higher than that without boundary member effect.
Genotype transposer: automated genotype manipulation for linkage disequilibrium analysis

UNLABELLED The purpose of this work is to provide the modern molecular geneticist with tools to perform more efficient and more accurate analysis of the genotype data they produce. By using Microsoft Excel macros written in Visual Basic, we can translate genotype data into a form readable by the versatile software 'Arlequin', read the Arlequin output, calculate statistics of linkage disequilibrium, and put the results in a format for viewing with the software 'GOLD'. AVAILABILITY The software is available by FTP at: ftp://xcsg.iarc. fr/cox/Genotype_Transposer/. SUPPLEMENTARY INFORMATION Detailed instruction and examples are available at: ftp://xcsg.iarc. fr/cox/Genotype&_Transposer/. Arlequin is available at: http://lgb.unige. ch/arlequin/. GOLD is available at: http://www.well. ox.ac. uk/asthma/GOLD/.
“Feeling more regret than I would have imagined”: Self-report and behavioral evidence

People tend to overestimate emotional responses to future events. This study examined whether such affective forecasting errors occur for feelings of regret, as measured by self-report and subsequent decision-making. Some participants played a pricing game and lost by a narrow or wide margin, while others were asked to imagine losing by such margins. Participants who experienced a narrow loss reported more regret than those who imagined a narrow loss. Furthermore, those experiencing a narrow loss behaved more cautiously in a subsequent gambling task. Thus, the study provides self-report and behavioral evidence for a reversal of the affective forecasting phenomenon for feelings of regret.
Selenium-Based S-Adenosylmethionine Analog Reveals the Mammalian Seven-Beta-Strand Methyltransferase METTL10 to Be an EF1A1 Lysine Methyltransferase

Lysine methylation has been extensively studied in histones, where it has been shown to provide specific epigenetic marks for the regulation of gene expression; however, the molecular mechanism and physiological function of lysine methylation in proteins other than histones remains to be fully addressed. To better understand the substrate diversity of lysine methylation, S-adenosylmethionine (SAM) derivatives with alkyne-moieties have been synthesized. A selenium-based SAM analog, propargylic Se-adenosyl-l-selenomethionine (ProSeAM), has a wide spectrum of reactivity against various lysine methyltransferases (KMTs) with sufficient stability to support enzymatic reactions in vitro. By using ProSeAM as a chemical probe for lysine methylation, we identified substrates for two seven-beta-strand KMTs, METTL21A and METTL10, on a proteomic scale in mammalian cells. METTL21A has been characterized as a heat shock protein (HSP)-70 methyltransferase. Mammalian METTL10 remains functionally uncharacterized, although its ortholog in yeast, See1, has been shown to methylate the translation elongation factor eEF1A. By using ProSeAM-mediated alkylation followed by purification and quantitative MS analysis, we confirmed that METTL21A labels HSP70 family proteins. Furthermore, we demonstrated that METTL10 also methylates the eukaryotic elongation factor EF1A1 in mammalian cells. Subsequent biochemical characterization revealed that METTL10 specifically trimethylates EF1A1 at lysine 318 and that siRNA-mediated knockdown of METTL10 decreases EF1A1 methylation levels in vivo. Thus, our study emphasizes the utility of the synthetic cofactor ProSeAM as a chemical probe for the identification of non-histone substrates of KMTs.
Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology*

Background: Rab small GTPases are membrane trafficking proteins in eukaryotes. Results: Comprehensive knockdown screening identified six Rab isoforms that are involved in regulating Golgi morphology in HeLa-S3 cells. Conclusion: Five of the six Rabs, including Rab2A and Rab2B, non-redundantly regulate Golgi morphology. A Rab2B-specific effector, GARI-L4, also regulates it. Significance: This is the first study to systematically analyze all human Rabs in the Golgi. Rab small GTPases are crucial regulators of the membrane traffic that maintains organelle identity and morphology. Several Rab isoforms are present in the Golgi, and it has been suggested that they regulate the compacted morphology of the Golgi in mammalian cells. However, the functional relationships among the Golgi-resident Rabs, e.g. whether they are functionally redundant or different, are poorly understood. In this study, we used specific siRNAs to perform genome-wide screening for human Rabs that are involved in Golgi morphology in HeLa-S3 cells. The results showed that knockdown of any one of the six Rab isoforms (Rab1A/1B/2A/2B/6B/8A) induced fragmentation of the Golgi in HeLa-S3 cells and that its phenotype was rescued by re-expression of their respective siRNA-resistant construct. We then performed systematic knockdown-rescue experiments in relation to each of the six Rabs. Interestingly, with the exception of the Rab8A knockdown, the Golgi fragmentation phenotype induced by knockdown of a single Rab isoform, e.g. Rab2B, was efficiently rescued by re-expression of its siRNA-resistant Rab alone, not by any of the other five Rabs, e.g. Rab2A, which is highly homologous to Rab2B, indicating that these Rab isoforms non-redundantly regulate Golgi morphology possibly through interaction with isoform-specific effector molecules. In addition, we identified Golgi-associated Rab2B interactor-like 4 (GARI-L4) as a novel Golgi-resident Rab2B-specific binding protein whose knockdown also induced fragmentation of the Golgi. Our findings suggest that the compacted Golgi morphology of mammalian cells is finely tuned by multiple sets of Rab (or Rab-effector complexes) that for the most part function independently.
Zinc Finger Protein 148 Is Dispensable for Primitive and Definitive Hematopoiesis in Mice

Hematopoiesis is regulated by transcription factors that induce cell fate and differentiation in hematopoietic stem cells into fully differentiated hematopoietic cell types. The transcription factor zinc finger protein 148 (Zfp148) interacts with the hematopoietic transcription factor Gata1 and has been implicated to play an important role in primitive and definitive hematopoiesis in zebra fish and mouse chimeras. We have recently created a gene-trap knockout mouse model deficient for Zfp148, opening up for analyses of hematopoiesis in a conventional loss-of-function model in vivo. Here, we show that Zfp148-deficient neonatal and adult mice have normal or slightly increased levels of hemoglobin, hematocrit, platelets and white blood cells, compared to wild type controls. Hematopoietic lineages in bone marrow, thymus and spleen from Zfp148 gt/gt mice were further investigated by flow cytometry. There were no differences in T-cells (CD4 and CD8 single positive cells, CD4 and CD8 double negative/positive cells) in either organ. However, the fraction of CD69- and B220-positive cells among lymphocytes in spleen was slightly lower at postnatal day 14 in Zfp148 gt/gt mice compared to wild type mice. Our results demonstrate that Zfp148-deficient mice generate normal mature hematopoietic populations thus challenging earlier studies indicating that Zfp148 plays a critical role during hematopoietic development.
Comparison of the Projected Results of Coarse Thermal Coal Cleaning in Various Gravity Separation Apparatuses

Gravity separation is considered as the most used processing technique for thermal coal cleaning. A broad variety of methods and machines was developed for that purpose. However, the performance of those apparatus under different conditions is not the same. In this article, the results of coarse thermal coal cleaning in various equipment were simulated by means of mathematical modelling. The comparison of obtained quantitative and qualitative indicators of gravity separation showed that for processing of difficult-to-wash thermal coal the heavy medium-based processes are more favorable, whereas for more easily washable coal jigging can be efficiently used as well.
Electrophysiological evidence of the time course of attentional bias in non-patients reporting symptoms of depression with and without co-occurring anxiety

Anxiety is characterized by attentional biases to threat, but findings are inconsistent for depression. To address this inconsistency, the present study systematically assessed the role of co-occurring anxiety in attentional bias in depression. In addition, the role of emotional valence, arousal, and gender was explored. Ninety-two non-patients completed the Penn State Worry Questionnaire (Meyer et al., 1990; Molina and Borkovec, 1994) and portions of the Mood and Anxiety Symptom Questionnaire (Watson et al., 1995a,1995b). Individuals reporting high levels of depression and low levels of anxiety (depression only), high levels of depression and anxiety (combined), or low levels of both (control) completed an emotion-word Stroop task during event-related brain potential recording. Pleasant and unpleasant words were matched on emotional arousal level. An attentional bias was not evident in the depression-only group. Women in the combined group had larger N200 amplitude for pleasant than unpleasant stimuli, and the combined group as a whole had larger right-lateralized P300 amplitude for pleasant than unpleasant stimuli, consistent with an early and later attentional bias that is specific to unpleasant valence in the combined group. Men in the control group had larger N200 amplitude for pleasant than unpleasant stimuli, consistent with an early attentional bias that is specific to pleasant valence. The present study indicates that the nature and time course of attention prompted by emotional valence and not arousal differentiates depression with and without anxiety, with some evidence of gender moderating early effects. Overall, results suggest that co-occurring anxiety is more important than previously acknowledged in demonstrating evidence of attentional biases in depression.
Mesua beccariana (Clusiaceae), A Source of Potential Anti-cancer Lead Compounds in Drug Discovery

An investigation on biologically active secondary metabolites from the stem bark of Mesua beccariana was carried out. A new cyclodione, mesuadione (1), along with several known constituents which are beccamarin (2), 2,5-dihydroxy-1,3,4-trimethoxy anthraquinone (3), 4-methoxy-1,3,5-trihydroxyanthraquinone (4), betulinic acid (5) and stigmasterol (6) were obtained from this ongoing research. Structures of these compounds were elucidated by extensive spectroscopic methods, including 1D and 2D-NMR, GC-MS, IR and UV techniques. Preliminary tests of the in vitro cytotoxic activities of all the isolated metabolites against a panel of human cancer cell lines Raji (lymphoma), SNU-1 (gastric carcinoma), K562 (erythroleukemia cells), LS-174T (colorectal adenocarcinoma), HeLa (cervical cells), SK-MEL-28 (malignant melanoma cells), NCI-H23 (lung adenocarcinoma), IMR-32 (neuroblastoma) and Hep-G2 (hepatocellular liver carcinoma) were carried out using an MTT assay. Mesuadione (1), beccamarin (2), betulinic acid (5) and stigmasterol (6) displayed strong inhibition of Raji cell proliferation, while the proliferation rate of SK-MEL-28 and HeLa were strongly inhibited by stigmasterol (6) and beccamarin (2), indicating these secondary metabolites could be anti-cancer lead compounds in drug discovery.
Planned Descemetorhexis Without Endothelial Keratoplasty in Eyes With Fuchs Corneal Endothelial Dystrophy

Purpose: The purpose of this study was to describe the results of a simple 6-mm central descemetorhexis in eyes with Fuchs corneal endothelial dystrophy. Methods: This prospective nonrandomized IRB approved pilot study was performed on 2 patients with coexisting Fuchs dystrophy and cataract who underwent combined phacoemulsification and descemetorhexis without endothelial transplantation. Results: In each patient, residual disciform stromal edema corresponded to the diameter of the descemetorhexis and led to an associated loss of visual acuity. Vision improved to 20/40 −1 in case 1 and to 150 −1 in case 2 during a follow-up period of 12 and 10 months, respectively. Endothelial cell repopulation of the descemetorhexis could not be confirmed because of the presence of persistent corneal edema. Conclusions: Because of the relatively poor postoperative visual acuity and slow visual recovery, a simple descemetorhexis is not an acceptable form of therapy for eyes with Fuchs corneal endothelial dystrophy.
Ambient effects on the laser durability of 157-nm optical coatings

We present results of the durability of antireflectance (AR) coatings under laser irradiation with emphasis on the interplay between coating materials and ambient. We find that introducing ppm-levels of water has a dramatic impact on the performance of certain coatings. In particular, no significant degradation of a coating was observed for up to 1MJ/cm2 dose in the presence of ~20 ppm H2O, whereas linear transmission drop of several percent was observed when irradiating a coating of similar design in <0.1 ppm H2O but under 1.5 ppm O2. Cycling water concentration on and off leads a corresponding cycling of transmission of the coatings. Adding water vapor to the ambient has a much greater benefit to coating durability than adding corresponding amounts of gas phase oxygen. In a series of experiments involving the same coating stack with different degrees of porosity of the outer layer, moisture was found to have the greatest impact on the most porous coating.
Magnetic Brainstem Stimulation of the Facial Nerve

Electrophysiological evaluation of cranial nerves provides information on its functional aspects and may be a valuable adjunct to imaging. In 10 normal subjects, we used transcranial magnetic stimulation with a double cone coil at the posterior head region to obtain orbicularis oris motor evoked potentials. Our findings suggest activation of descending facial fibers proximal to brainstem motoneurons. This method is advocated as an adjunct in the electrodiagnostic workup of facial nerve dysfunction.
Diffusion and defect reactions between donors, C, and vacancies in Ge. II. Atomistic calculations of related complexes

Electronic structure calculations are used to study the stability, concentration, and migration of vacancy-donor (phosphorus, arsenic, and antimony) complexes in germanium, in the presence of carbon. The association of carbon with mobile vacancy-donor pairs can lead to energetically favorable and relatively immobile complexes. It is predicted that the complexes formed between lattice vacancies, carbon, and antimony substitutional atoms are more stable and less mobile compared to complexes composed of vacancies, carbon, and phosphorus or arsenic atoms. Then, with the use of mass action analysis, the relative concentrations of the most important complexes are calculated, which depend also on their relative stability not just their absolute stability. Overall, the theoretical predictions are consistent with experimental results, which determined that the diffusion of vacancy-donor defects is retarded in the presence of carbon, especially in samples with a high concentration of carbon. In addition, the calculations provide information on the structure and the equilibrium concentration of the most important complexes and details of their association energies.