Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparative Genomics and Biosynthetic Potential Analysis of Two Lichen-Isolated Amycolatopsis Strains

Actinomycetes have been extensively exploited as one of the most prolific secondary metabolite-producer sources and continue to be in the focus of interest in the constant search of novel bioactive compounds. The availability of less expensive next generation genome sequencing techniques has not only confirmed the extraordinary richness and broad distribution of silent natural product biosynthetic gene clusters among these bacterial genomes, but also has allowed the incorporation of genomics in bacterial taxonomy and systematics. As part of our efforts to isolate novel strains from unique environments, we explored lichen-associated microbial communities as unique assemblages to be studied as potential sources of novel bioactive natural products with application in biotechnology and drug discovery. In this work, we have studied the whole genome sequences of two new Amycolatopsis strains (CA-126428 and CA-128772) isolated from tropical lichens, and performed a comparative genomic analysis with 41 publicly available Amycolatopsis genomes. This work has not only permitted to infer and discuss their taxonomic position on the basis of the different phylogenetic approaches used, but has also allowed to assess the richness and uniqueness of the biosynthetic pathways associated to primary and secondary metabolism, and to provide a first insight on the potential role of these bacteria in the lichen-associated microbial community.
Releasability of asbestos fibers from weathered roof cement

ABSTRACT Chrysotile asbestos fibers were added to roofing products, including roof cement, for several decades. The fibers were described as “encapsulated” and therefore incapable of being released, an assertion that is disproved by the study reported herein. Three test panels of roof cement from the original container were exposed to ambient weathering in 2015 and 2016. Two panels were then sampled using the ASTM D5755 microvacuum method. Sampling revealed a light brown sub-layer under the dark brown surface layer, both of which crumbled and became friable during sampling. Analysis of the microvacuum samples with transmission electron microscopy showed that the material on the 2 panels contained 4,432,000 and 3,320,000 asbestos structures per cm² with nearly all of the structures consisting of fibers less than 5 µm long. Energy dispersive spectrometry determined that none of the fibers reported were coated with asphalt. The presence of free fibers was confirmed by direct examination of the surfaces of the panels and of dust released from handling the panels via scanning electron microscopy. This study confirmed the releasability of uncoated asbestos fibers from dried roof cement that was indicated in 2 previous studies published in 2007 and 2010. These results suggest that the finding of the Fifth Circuit Court in 1997 that uncoated airborne asbestos fibers cannot be released from roof cement, and therefore do not present a potential exposure by inhalation, was erroneous in retrospect. The exemption of roof cement from regulation under the Occupational Safety and Health Administration Construction Industry Standard for asbestos by the Court should not be relied on by employers of workers who remove weathered asbestos-containing roof cement, and precautions should be taken against exposure to airborne asbestos fibers during this work.
An Electronic Publication Dedicated to Early Stellar Evolution and Molecular Clouds Episodic Formation of Cometary Material in the Outburst of a Young Sun-like Star Protoplanetary Disk Structures in Ophiuchus

The Solar System originated in a cloud of interstellar gas and dust. The dust is in the form of amorphous silicate particles and carbonaceous dust. The composition of cometary material, however, shows that a significant fraction of the amorphous silicate dust was transformed into crystalline form during the early evolution of the protosolar nebula. How and when this transformation happened has been a question of debate, with the main options being heating by the young Sun and shock heating. Here we report mid-infrared features in the outburst spectrum of the young Sun-like star EX Lupi that were not present in quiescence. We attribute them to crystalline forsterite. We conclude that the crystals were produced through thermal annealing in the surface layer of the inner disk by heat from the outburst, a process that has hitherto not been considered. The observed lack of cold crystals excludes shock heating at larger radii. We present the results of a high angular resolution (0.3 arcsec ≈ 40 AU) Submillimeter Array survey of the 345 GHz (870 µm) thermal continuum emission from 9 of the brightest, and therefore most massive, circumstellar disks in the ∼1 Myr-old Ophiuchus star-forming region. Using two-dimensional radiative transfer calculations, we simultaneously fit the observed continuum visibilities and broadband spectral energy distribution for each disk with a parametric structure model. Compared to previous millimeter studies, this survey includes significant upgrades in modeling, data quality, and angular resolution that provide improved constraints on key structure parameters, particularly those that characterize the spatial distribution of mass in the disks. In the context of a surface density profile motivated by similarity solutions for viscous accretion disks, Σ ∝ (R/R c) −γ exp [−(R/R c) 2−γ ], the best-fit models for the sample disks have characteristic radii R c ≈ 20-200 AU, high disk masses M d ≈ 0.005-0.14 M ⊙ (a sample selection bias), and a narrow range of radial Σ gradients (γ ≈ 0.4-1.0) around a median γ = 0.9. These density structures are used in conjunction with accretion rate estimates from the literature to help characterize the viscous evolution of the disk material. Using the standard prescription for disk viscosities, those combined constraints indicate that α ≈ 0.0005
A Novel Approach to CAFO Odor Assessment: Odorant Field Collection by Sorbent Tube / Thermal Reconstitution in the Laboratory

Historically, odor emission monitoring of high density livestock operations has been limited to direct, whole-air assessment utilizing remote site sampling by plastic bags, human sensory panelists and dynamic dilution olfactometry. On-going efforts by these authors are directed at enabling the translation of these sensory ‘only’ monitoring protocols to instrument ‘primarily, with sensory oversight’ alternatives. This current work attempts to address the latter requirement for sensory oversight and the associated need for improved methods of remote site whole-air sample collection. Published works by these authors as well as others places in serious question the appropriateness of the use of plastic bags for the challenge of CAFO odor assessment; especially relative to sample points at increasing downwind distance from the source. Concerns increase due to the associated natural dilution effects relative to priority semi-volatile odorants. This current submission reports on the progress to date in the development and evaluation of alternative whole-air sampling strategies which attempt to address the primary limitation of plastic sampling bags; the adsorption loss-to-wall of high impact semi-volatile odorants such as p-cresol. In this approach the actual field air sampling is carried out utilizing sorbent tubes for on-site collection of the volatiles/odorants from a measured volume of air. These sorbent tube collections are then transported to the laboratory for reconstitution within a heat traced, passivated and piston-displaced vessel prior to composite odor assessment. The sample reconstitution process is accomplished simply by thermally desorbing the collected odorants into a flowing diluent gas stream and making up to final volume to yield a match of the originally sampled environment.
Investigating the role of perception of aging and associated factors in death anxiety among the elderly

Background and objectives The world’s growing elderly population highlights the necessity for further attention to the psychological problems of the elderly, such as death anxiety. Analysis of the elderly’s perception of aging and associated factors can contribute to prediction of their future physical and mental health. The purpose of this study was to determine the relationship of perception of aging, and a group of demographic factors, with death anxiety in the elderly living in Gonabad, Iran. Subjects and methods This analytical descriptive study was conducted on 330 elderly residents of Gonabad, who were selected by stratified random sampling. Research tools were a standard demographic questionnaire, Barker’s Brief Aging Perceptions Questionnaire, and Collett–Lester Fear of Death Scale. Data were collected by interview of respondents at their home. Data analysis was carried out in SPSS 16 using descriptive statistics, analysis of variance, Pearson’s test, independent t-test, and linear regression. Results Respondents had a mean age of 73.97±7.68 years, 42.4% of respondents were men and 57.6% were women. The total Barker’s Brief Aging Perceptions Questionnaire score was 63.18±8.75, with the highest score (17.10±4.70) associated with negative consequences and control. The total Collett–Lester Fear of Death Scale score was 128±14.80, with the highest score (35.13±4.06) pertaining to the subscale fear of other people’s death. Regression results indicated that the death anxiety score was predictable according to the age and all dimensions of perception of aging, except for the consequences and negative control dimension. Conclusion Age and the perception of aging are good predictors of death anxiety. The authors recommend further research on the determinants of death anxiety in the elderly and the development of a comprehensive care plan to reduce this anxiety among Iranian elderly.
The Potential of Triterpenoids from Loquat Leaves (Eriobotrya japonica) for Prevention and Treatment of Skin Disorder

The leaves of loquat (Eriobotrya japonica) possess high medicinal value and have been used as traditional medicines. However, there are no evidence-based studies on the skin-care effects of E. japonica leaves. To explore new biological activities of E. japonica leaves against skin disorder and to gain a better understanding of the chemical components associated with bioactivities, we evaluated 18 triterpenoids from E. japonica leaves on anti-melanogenesis, anti-acne, anti-allergy and anti-aging activities. Our results revealed that eight compounds showed anti-melanogenesis activity, of which ursolic acid (1) and maslinic acid (7) were the most potent with the similar selective index to that of arbutin. Structure–activity relationship and possible mechanism of active compounds were proposed. Twelve compounds exhibited anti-acne effect; ursolic acid (1), maslinic acid (7), corosolic acid (8) and euscaphic acid (12) showed highest activities against P. acnes. Four compounds displayed anti-allergy and anti-inflammatory activity; 3-epicorosolic acid (9) and euscaphic acid (12) showed marked activity against β-hexosaminidase release. Finally, ursolic acid (1), pomolic acid (10), colosolic acid (8) and its methylated derivative (6) exhibited the highest anti-aging activity by stimulating collagen and hyaluronic acid (HA) production. Our findings provide valuable evidence that E. japonica leaves have potential applications as ingredients of function foods or cosmetics for health benefits and a number of triterpenoids may play an important role in these bioactivities.
Emotional arousal deficit or emotional regulation bias? An electrophysiological study of age-related differences in emotion perception

ABSTRACT Background/Study context: Adult age differences in emotion processing have been attributed to age-related decline in earlier emotional perception and age-related bias in later emotional regulation. Yet, the relationship between the processes of early emotion perception and bias in emotional regulation and their influence on behavioral outcomes remains unclear. Event-related potentials (ERPs) have the temporal precision to allow for the online measure of neurophysiological activity and provide potential insight into the complex dynamics of emotion processing and aging. Methods: ERPs were used as the primary measure to examine the hypotheses that younger adults will differ in emotional arousal and emotional bias as represented by the early P1 waveform and later P3 waveform, respectively. Thirty-two younger and older adults (16 each) performed a facial emotion discrimination task in which they identified standardized angry, happy, or neutral expressions of faces from the NimStim database. Results: Younger adults showed a greater P1 ERP for angry faces relative to happy faces at parietal channels, while older adults did not exhibit any emotional modulation of the P1. In contrast, both younger and older adults showed a greater late P3 ERP for angry faces compared to happy faces. Conclusion: The authors’ results provide evidence for an age-related deficit in early emotion perception and autonomic arousal. Younger adults, but not older adults, exhibited a pattern of neurophysiological activity believed to reflect preconscious and reflexive identification of threat. Despite these age group differences in early emotion processing, younger and older adults did not exhibit differences in neurophysiological processes believed to reflect emotion regulation.
Direct adjustment for confounding by smoking reduces radiation-related cancer risk estimates of mortality among male nuclear workers in Japan, 1999–2010

A causal relationship between protracted exposure to low-dose rate radiation and health effects remains unclear despite extensive international studies of nuclear workers. One potential reason is that radiation epidemiological studies that adjust for tobacco smoking, which heavily influences mortality, have been limited. In the present study, we examined radiation-related cancer risk by directly assessing the possible confounding effect of smoking, using data from two questionnaire surveys performed among Japanese nuclear workers in 1997 and 2003. Mortality follow-up was carried out for 71 733 male respondents for an average of 8.2 years during the observation period of 1999–2010. The mean cumulative dose was 25.5 mSv at the end of the follow-up period. Estimates of excess relative risk per Sv (ERRs/Sv) were obtained by Poisson regression. By adjusting for smoking directly on the basis of a linear dose-response model, we quantified the confounding effects of smoking on radiation risks. Statistically significant ERRs/Sv were found for all causes, all diseases, all non-cancer diseases, and liver cancer: 0.97 (90% confidence interval: 0.23, 1.78), 1.32 (0.40, 2.34), 1.87 (0.47, 3.49), and 4.78 (0.09, 11.68), respectively, without adjustment for smoking. However, the ERRs/Sv were no longer statistically significant after adjustment for smoking: 0.45 (−0.22, 1.19), 0.77 (−0.08, 1.72), 1.28 (−0.03, 2.79), and 3.89 (−0.46, 10.34), respectively. The ERRs/Sv for all cancers excluding leukaemia and lung cancer were not significant before adjustment for smoking, but declined after adjustment for smoking. The present study demonstrates that in this cohort of workers, smoking heavily distorts radiation risk estimates of mortality. The possibility of confounding by smoking depends on how strongly smoking is correlated with radiation exposure. If a correlation between smoking and radiation dose is suggested, smoking is an important confounder when assessing the radiation and health risks.
A New Look to a Classic Issue: Reasoning and Academic Achievement at Secondary School

Higher-order thinking abilities such as abstract reasoning and meaningful school learning occur sequentially. The fulfillment of these tasks demands that people activate and use all of their working memory resources in a controlled and supervised way. The aims of this work were: (a) to study the interplay between two new reasoning measures, one mathematical (Cognitive Reflection Test) and the other verbal (Deductive Reasoning Test), and a third classical visuo-spatial reasoning measure (Raven Progressive Matrices Test); and (b) to investigate the relationship between these measures and academic achievement. Fifty-one 4th grade secondary school students participated in the experiment and completed the three reasoning tests. Academic achievement measures were the final numerical scores in seven basic subjects. The results demonstrated that cognitive reflection, visual, and verbal reasoning are intimately related and predicts academic achievement. This work confirms that abstract reasoning constitutes the most important higher-order cognitive ability that underlies academic achievement. It also reveals the importance of dual processes, verbal deduction and metacognition in ordinary teaching and learning at school.
Thermal Analysis of Lignocellulosic Materials

Abstract Thermal analysis has recently become prominent, particularly in applications to fibers, plastics, and other synthetic polymeric materials. Thermal analysis may be defined as a set of techniques used to describe the physical or chemical changes associated with substances as a function of temperature [1]. Thermograms have been used to determine thermodynamic and kinetic parameters associated with the chemical changes which occur upon heating, the heat capacity of a substance, first- and second-order transition temperatures, latent heats of transformation, and effects of radiation or additives on natural and synthetic polymers. Particularly successful appears to be the simultaneous use of two or more thermal analytical techniques or the combination of thermal analytical and spectroscopic techniques (for example, infrared, electronic, and nuclear magnetic resonance analysis). The increasing commercial importance of polymers and the commercial manufacture of thermal analytical instruments have resulte.
Association of the long non-coding RNA MALAT1 with the polycomb repressive complex pathway in T and NK cell lymphoma

Recently, various long non-coding RNAs (lncRNAs) have been reported to have significant therapeutic or prognostic value. However, the expression of lncRNAs has not been investigated in T and NK cell lymphoma. Thus, we evaluated the biological and prognostic role of lncRNAs related to the polycomb repressive complex (PRC) and PRC markers in tissue samples and cell lines of T and NK cell lymphoma. Among the tested lncRNAs, MALAT1 was most highly expressed in clinical samples and cell lines. High expression of MALAT1 as well as BMI1 was related to poor prognosis in patients with mature T cell lymphoma. In the tissue samples, BMI1 expression showed a positive correlation with EZH2, SUZ12, H3K27me3, and MALAT1. Multiple linear regression analysis showed that BMI1 expression was independently associated with H3K27me3. Direct binding of MALAT1 to the PRC2 components (EZH2 and SUZ12) was observed in a T cell lymphoma cell line; however, no direct binding of MALAT1 with H3K27me3 and BMI1 (a PRC1 component) was observed. In T and NK cell lymphomas, MALAT1 was related to poor prognosis. MALAT1 directly binds to EZH2 and SUZ12, and BMI1 activation may be induced possibly through H3K27me3.
Molecular Docking and Anticonvulsant Activity of Newly Synthesized Quinazoline Derivatives

A new series of quinazoline-4(3H)-ones are evaluated for anticonvulsant activity. After intraperitoneal (ip) injection to albino mice at a dose of 100 mg/kg body weight, synthesized quinazolin-4(3H)-ones (1–24) were examined in the maximal electroshock (MES) induced seizures and subcutaneous pentylenetetrazole (scPTZ) induced seizure models in mice. The Rotarod method was applied to determine the neurotoxicity. Most of the compounds displayed anticonvulsant activity in the scPTZ screen at a dose range of 0.204–0.376 mmol/mL. Out of twenty-four, compounds 8, 13 and 19 proved to be the most active with a remarkable protection (100%) against PTZ induced convulsions and four times more potent activity than ethosuximide. The structure-activity relationship concluded valuable pharmacophoric information, which was confirmed by the molecular docking studies using the target enzyme human carbon anhydrase II (HCA II). The studied quinazoline analogues suggested that the butyl substitution at position 3 has a significant effect on preventing the spread of seizure discharge and on raising the seizure threshold. However, benzyl substitution at position 3 has shown a strong anticonvulsant activity but with less seizure prevention compared to the butyl substitution.
Self-Attentional Acoustic Models

Self-attention is a method of encoding sequences of vectors by relating these vectors to each-other based on pairwise similarities. These models have recently shown promising results for modeling discrete sequences, but they are non-trivial to apply to acoustic modeling due to computational and modeling issues. In this paper, we apply self-attention to acoustic modeling, proposing several improvements to mitigate these issues: First, self-attention memory grows quadratically in the sequence length, which we address through a downsampling technique. Second, we find that previous approaches to incorporate position information into the model are unsuitable and explore other representations and hybrid models to this end. Third, to stress the importance of local context in the acoustic signal, we propose a Gaussian biasing approach that allows explicit control over the context range. Experiments find that our model approaches a strong baseline based on LSTMs with network-in-network connections while being much faster to compute. Besides speed, we find that interpretability is a strength of self-attentional acoustic models, and demonstrate that self-attention heads learn a linguistically plausible division of labor.
Investigational opioid antagonists for treating opioid-induced bowel dysfunction

ABSTRACT Introduction: Opioids have been highlighted for their role in pain relief among cancer and non-cancer patients. Novel agents have been investigated to reduce opioid-induced constipation (OIC) as the main adverse effect that may lead to treatment discontinuation. Development of peripherally acting mu-opioid receptor antagonists (PAMORA) has resulted in a novel approach to preserve the efficacy of pain control along with less OIC. Areas covered: Clinical evidence for investigational PAMORAs was reviewed and clinical trials on investigational agents to reduce OIC were included. TD-1211 is currently being evaluated in Phase II clinical trial. Oxycodone-naltrexone and ADL-5945 went through Phase III clinical trials, but have been discontinued. Expert opinion: There is a substantial need to develop agents with specific pharmacokinetic properties to meet the needs of patients with underlying diseases. Holding the efficacy of a medicine with the highest selectivity on targeted receptors and the least adverse effects is the main approach in upcoming investigations to improve patients’ quality of life (QoL). Novel agents to reduce opioid-induced bowel dysfunction (OIBD) that do not reverse peripherally mediated pain analgesia are of great interest. Direct comparison of available agents in this field is lacking in the literature.