Miracle-dz/UniFAR-FTUs huggingface dataset:
Axonally Derived Neuregulin-1 Is Required for Remyelination and Regeneration after Nerve Injury in Adulthood

Neuregulin-1 (NRG1) plays a crucial role in axoglial signaling during the development of the peripheral nervous system, but its importance in adulthood after peripheral nerve injury remains unclear. We used single-neuron labeling with inducible Cre-mediated knock-out animals, which enabled visualization of a subset of adult myelinated sensory and motoneurons neurons in which Nrg1 was inducibly mutated by tamoxifen treatment. In uninjured mice, NRG1-deficient axons and the associated myelin sheath were normal, and the neuromuscular junction demonstrated normal apposition of presynaptic and postsynaptic components. After sciatic nerve crush, NRG1 ablation resulted in severe defects in remyelination: axons were either hypomyelinated or had no myelin sheath. NRG1-deficient axons were also found to regenerate at a slower rate. After nerve injury, the neuromuscular junction was reinnervated, but excess terminal sprouting was observed. Juxtacrine Neuregulin-1 signaling is therefore dispensable for maintenance of the myelin sheath in adult animals but has a key role in reparative processes after nerve injury.
The Rab11 Pathway Is Required for Influenza A Virus Budding and Filament Formation

ABSTRACT Influenza A virus buds through the apical plasma membrane, forming enveloped virus particles that can take the shape of pleomorphic spheres or vastly elongated filaments. For either type of virion, the factors responsible for separation of viral and cell membranes are not known. We find that cellular Rab11 (a small GTP-binding protein involved in endocytic recycling) and Rab11-family interacting protein 3 ([FIP3] which plays a role in membrane trafficking and regulation of actin dynamics) are both required to support the formation of filamentous virions, while Rab11 is additionally involved in the final budding step of spherical particles. Cells transfected with Rab11 GTP-cycling mutants or depleted of Rab11 or FIP3 content by small interfering RNA treatment lost the ability to form virus filaments. Depletion of Rab11 resulted in up to a 100-fold decrease in titer of spherical virus released from cells. Scanning electron microscopy of Rab11-depleted cells showed high densities of virus particles apparently stalled in the process of budding. Transmission electron microscopy of thin sections confirmed that Rab11 depletion resulted in significant numbers of abnormally formed virus particles that had failed to pinch off from the plasma membrane. Based on these findings, we see a clear role for a Rab11-mediated pathway in influenza virus morphogenesis and budding.
Targeted delivery of nerve growth factor via encapsulated cell biodelivery in Alzheimer disease: a technology platform for restorative neurosurgery.

OBJECT The authors describe the first clinical trial with encapsulated cell biodelivery (ECB) implants that deliver nerve growth factor (NGF) to the cholinergic basal forebrain with the intention of halting the degeneration of cholinergic neurons and the associated cognitive decline in patients with Alzheimer disease (AD). The NsG0202 implant (NsGene A/S) consists of an NGF-producing, genetically engineered human cell line encapsulated behind a semipermeable hollow fiber membrane that allows the influx of nutrients and the efflux of NGF. The centimeter-long capsule is attached to an inert polymer tether that is used to guide the capsule to the target via stereotactic techniques and is anchored to the skull at the bur hole. METHODS Six patients with mild to moderate AD were included in this Phase Ib open-label safety study and were divided into 2 dose cohorts. The first cohort of 3 patients received single implants targeting the basal nucleus of Meynert (Ch4 region) bilaterally (2 implants per patient), and after a safety evaluation, a second cohort of 3 patients received bilateral implants (a total of 4 implants per patient) targeting both the Ch4 region and the vertical limb of the diagonal band of Broca (Ch2 region). Stereotactic implantation of the devices was successfully accomplished in all patients. Despite extensive brain atrophy, all targets could be reached without traversing sulci, the insula, or lateral ventricles. RESULTS Postoperative CT scans allowed visualization of the barium-impregnated tethers, and fusion of the scans with stereotactic MR images scan was used to verify the intended positions of the implants. Follow-up MRI at 3 and 12 months postimplantation showed no evidence of inflammation or device displacement. At 12 months, implants were successfully retrieved, and low but persistent NGF secretion was detected in half of the patients. CONCLUSIONS With refinement, the ECB technology is positioned to become an important therapeutic platform in restorative neurosurgery and, in combination with other therapeutic factors, may be relevant for the treatment of a variety of neurological disorders. Clinical trial registration no.: NCT01163825.
Two-Dimensional Multivariate Parametric Models for Radar Applications—Part I: Maximum-Entropy Extensions for Toeplitz-Block Matrices

In a series of two papers, a new class of parametric models for two-dimensional multivariate (matrix-valued, space-time) adaptive processing is introduced. This class is based on the maximum-entropy extension and/or completion of partially specified matrix-valued Hermitian covariance matrices in both the space and time dimensions. This first paper considers the more restricted class of Toeplitz Hermitian covariance matrices that model stationary clutter. If the clutter is stationary only in time then we deal with a Toeplitz-block matrix, whereas clutter that is stationary in time and space is described by a Toeplitz-block-Toeplitz matrix. We first derive exact expressions for this new class of 2-D models that act as approximations for the unknown true covariance matrix. Second, we propose suboptimal (but computationally simpler) relaxed 2-D time-varying autoregressive models (ldquorelaxationsrdquo) that directly use the non-Toeplitz Hermitian sample covariance matrix. The high efficiency of these parametric models is illustrated by simulation results using true ground-clutter covariance matrices provided by the DARPA KASSPER Dataset 1, which is a trusted phenomenological airborne radar model, and a complementary AFRL dataset.
A meta-analysis of executive components of working memory.

Working memory (WM) enables the online maintenance and manipulation of information and is central to intelligent cognitive functioning. Much research has investigated executive processes of WM in order to understand the operations that make WM "work. " However, there is yet little consensus regarding how executive processes of WM are organized. Here, we used quantitative meta-analysis to summarize data from 36 experiments that examined executive processes of WM. Experiments were categorized into 4 component functions central to WM: protecting WM from external distraction (distractor resistance), preventing irrelevant memories from intruding into WM (intrusion resistance), shifting attention within WM (shifting), and updating the contents of WM (updating). Data were also sorted by content (verbal, spatial, object). Meta-analytic results suggested that rather than dissociating into distinct functions, 2 separate frontal regions were recruited across diverse executive demands. One region was located dorsally in the caudal superior frontal sulcus and was especially sensitive to spatial content. The other was located laterally in the midlateral prefrontal cortex and showed sensitivity to nonspatial content. We propose that dorsal-"where"/ventral-"what" frameworks that have been applied to WM maintenance also apply to executive processes of WM. Hence, WM can largely be simplified to a dual selection model.
The salmon vertebral body develops through mineralization of two preformed tissues that are encompassed by two layers of bone

The teleost backbone consists of amphicoelous vertebrae and intervertebral ligaments, both of which include notochord‐derived structures. On the basis of a sequential developmental study of the vertebral column of Atlantic salmon (Salmo salar L.) from the egg stage up to early fry stage (300–2500 day‐degrees) we show that the vertebral body consists of four layers or compartments, two of which are formed through mineralization of preformed collagenous tissue (the notochordal sheath and the intervertebral ligament) and two of which are formed through ossification. The three inner layers have ordered lamellar collagen matrixes, which alternate perpendicularly from layer to layer, whereas the outer layer consists of cancellous bone with a woven matrix. The bone layers also differ in osteocyte content. In this study we describe the structural details of the layers, and their modes of formation. The results are compared with previous descriptions, and possible phylogenetic implications are discussed.
Development and aging of cell topography in the human retinal pigment epithelium.

PURPOSE To determine how regional cell density of this tissue changes with age, the authors examined the topography of the human retinal pigment epithelium (RPE) in wholemounted tissue obtained from eyes aged 12 to 89 years, donated for corneas. METHODS The RPE, with choroid attached, was wholemounted and stained with cresyl violet. From these preparations, the authors analyzed retinal area, RPE cell number, and cell density. RESULTS Retinal pigment epithelial cell number is highly variable between persons but does not appear to be age related. Retinal area increases until approximately 30 years of age, but beyond this age individual variation masks further enlargement. The distinctive topography of the RPE changes markedly with age. There is a modification from the relatively homogeneous cell density distribution in the youngest retinas examined toward a more heterogeneous pattern in older retinas. From mid-adolescence, a band of larger cells appears at the extreme periphery, adjacent to the ora serrata, which gradually widens so that by 90 years of age, it occupies the outermost 30% of the retinal area. Cell density is highest in the central temporal retina, adjacent to the macula in the neural retina, throughout life. Cell density values in this region increase slightly with age, and the difference between this and surrounding regions becomes more marked with age. CONCLUSIONS With no marked change in total cell number, peripheral RPE in humans enlarges in area throughout life, but the RPE in more central regions decreases in area.
Robotic Approach In Rectal Cancer Versus Laparoscopic Approach: Preliminary Results Of A Prospective Comparative Study

The conventional laparoscopic approach to rectal surgery has several limitations, and therefore many colorectal surgeons have great expectations for the robotic surgical system as an alternative modality in overcoming challenges of laparoscopic surgery and thus enhancing oncologic and functional outcomes. The aim of this study was to evaluate the feasibility and short-term outcomes of robotic surgery for colorectal cancer as initial cases, compared with conventional laparoscopic surgery. From July 2015 to October 2015, fifteen patients with left-sided colon and rectal cancer underwent robotic surgery, and we compare with 20 patients received conventional laparoscopic surgery selected from our database of 153 patients with colorectal cancer operated in the last five years. Both groups were balanced in terms of age, gender, American Society of Anesthesiologists (ASA) score, body mass index (BMI), operative history, TNM staging, and tumor location using the propensity score matching method. The perioperative results included the operative time, amount of estimated blood loss, the need for open conversion or further surgery, complications, flatus passage, the length of postoperative hospital stay, and the number of retrieved lymph nodes. There were no significant differences in the short-term outcomes between the robotic surgery group and the conventional laparoscopic surgery group. However, the operative time was significantly longer in the robotic surgery group than in the conventional laparoscopic surgery group. There were no significant differences between the robotic surgery group and the conventional laparoscopic surgery group with respect to short-term outcomes, with the exception of the operative time. Our early experience indicates that robotic surgery is a promising tool, particularly in patients with rectal cancer.
Molecular Analysis of the Gene Encoding F420-Dependent Glucose-6-Phosphate Dehydrogenase fromMycobacterium smegmatis

ABSTRACT The gene fgd, which codes for F420-dependent glucose-6-phosphate dehydrogenase (FGD), was cloned from Mycobacterium smegmatis, and its sequence was determined and analyzed. A homolog of FGD which has a very high similarity to the M. smegmatis FGD-derived amino acid sequence was identified in Mycobacterium tuberculosis. FGD showed significant homology with F420-dependentN5,N10-methylene-tetrahydromethanopterin reductase (MER) from methanogenic archaea and with several hypothetical proteins from M. tuberculosis andArchaeoglobus fulgidus, but FGD showed no significant homology with NADP-dependent glucose-6-phosphate dehydrogenases. Multiple alignment of FGD and MER proteins revealed four conserved consensus sequences. Multiple alignment of FGD with the hypothetical proteins also revealed portions of the same conserved sequences. Moderately high levels of FGD were expressed inEscherichia coli BL21(DE3) carrying fgd in pBluescript.
Analgesic use and risk of renal cell carcinoma: A case‐control, cohort and meta‐analytic assessment

Analgesics are the most commonly consumed drugs worldwide. Evidence that analgesics increase kidney cancer risk has been mixed. We investigated the association between renal cell carcinoma (RCC) and analgesic use in a large population‐based case‐control study and a post‐trial observational cohort study. Findings were used to update a recent meta‐analytic review. We analyzed data from 1,217 RCC cases and 1,235 controls in the US Kidney Cancer Study and 98,807 participants in the US Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial (PLCO: n = 137 RCCs). Self‐reported acetaminophen, aspirin and nonsteroid anti‐inflammatory drug (NSAID) use and duration information was assessed in relation to RCC. For the US Kidney Cancer Study, we calculated odds ratios (ORs) and 95% confidence intervals (CIs) using unconditional logistic regression. For PLCO, we computed hazard ratios (HRs) and 95%CIs using Cox regression. Among case‐control participants, RCC risk was associated with over‐the‐counter acetaminophen use (OR = 1.35, 95%CI = 1.01–1.83). There was a positive trend with increasing duration (p‐trend = 0.01), with a two‐fold risk for use ≥10 years (OR = 2.01, 95%CI = 1.30–3.12). No association with prescription acetaminophen use was detected. In PLCO, acetaminophen use was also associated with increased RCC risk (HR = 1.68, 95%CI = 1.19‐2.39), although elevated risk was absent among the few long‐term users. No association with RCC risk was detected for aspirin or NSAIDs use in either study. An association between acetaminophen use and kidney cancer was supported by meta‐analytic cohort (n = 4; summary relative risk = 1.34; 95%CI = 1.13–1.59; p‐heterogeneity = 0.40) and case‐control (n = 9, summary OR = 1.20; 95%CI = 1.01‐1.42; p‐heterogeneity = 0.05) findings. In brief, acetaminophen use may increase the risk of developing RCC.
Microfluidics for Processing Surfaces and Miniaturizing Biological Assays

This review is an account of our efforts to develop a versatile and flexible microfluidic technology for surface‐processing applications and miniaturizing biological assays. The review is presented in the context of current trends in microfluidic technology and addresses some of the major challenges for confining chemical and biochemical processes on surfaces: the sealing of a microchannel with a surface, the world‐to‐chip interface, the displacement of liquids in small conduits, the sequential delivery of multiple solutions, the accurate patterning of surfaces, the coincident detection of various analytes, and the detection of analytes in a small and dilute sample. Our solutions to these problems include the use of reversible sealing, capillary phenomena for powering and controlling liquid transport, and non‐contact microfluidics for spotting and drawing (on surfaces) with flow conditions. These solutions offer many advantages over conventional techniques for handling minute amounts of liquids and may find applications in lithography, biopatterning (e.g., the patterning of biomolecules), diagnostics, drug discovery, and also cellular assays.
Thyroid cancer in childhood: a retrospective review of childhood course.

BACKGROUND Thyroid cancer (TC) is an uncommon childhood malignancy, but the incidence may be increasing. Recent American Thyroid Association guidelines focus primarily on adult data. Natural history studies of TC in childhood are important to determine outcomes. The objectives of this study were to describe the demographics and outcomes in children with TC treated at The Hospital for Sick Children, Toronto, from 1983 to 2006. We hypothesized that childhood TC was increasing at our institution. METHODS Cases of papillary TC (PTC) (including follicular variant PTC) and follicular TC (FTC) were identified from pathology databases. Chart review was performed, and data were extracted on clinical, treatment, and outcome variables. RESULTS Sixty-one cases were identified, and complete data were available in 54, including 36 girls and 18 boys. There was no statistical change in numbers of cases diagnosed yearly during the study period. Younger children were more likely to have metastases at presentation or during follow-up. Pathological TC diagnosis included 40 PTC, 1 diffuse-sclerosing papillary, 7 follicular variant PTC, and 6 FTC. There was no difference in pathology findings between children less than or greater than 10 years old. Five patients had a history of previous malignancy, and five had a history of previous thyroid conditions. Three patients were born in areas of high TC endemnicity. Twenty-three patients had thyroiditis on pathology examination. All patients underwent total thyroidectomy, and 53/54 patients received therapeutic radioactive iodine ablation. Twenty-seven patients had metastases at presentation (19 lymph nodes only, 2 lung only, and 6 lymph node and distant) and 6 developed distant metastases during follow-up (3 lung, 2 thymus, and 1 paraspinal). Male sex was associated with development of metastases during follow-up. On multiple regression, tumor size was predicted positively by PTC but not by age, sex, or metastases at presentation or during follow-up. CONCLUSION We did not find evidence of increasing numbers of cases of TC diagnosed yearly during the study period, or difference in tumor aggressiveness, or between outcomes in children aged less than or greater than 10 years. Children with metastases at presentation or during follow-up were likely to be younger than children without metastases. There is a need for prospective, collaborative multicenter studies of TC.
Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen

Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.
Towards ambulatory brain-computer interfaces: a pilot study with P300 signals

Brain-Computer Interfaces (BCI) are communication systems that enable users to interact with computers using only brain activity. This activity is generally measured by ElectroEncephaloGraphy (EEG). A major limitation of BCI is the electrical sensitivity of EEG which causes severe deterioration of the signals when the user is moving. This constrains current EEG-based BCI to be used only by sitting and still subjects, hence limiting the use of BCI for applications such as video games. In this paper, we proposed a feasibility study to discover whether a BCI system, here based on the P300 brain signal, could be used with a moving subject. We recorded EEG signals from 5 users in 3 conditions: sitting, standing and walking. Analysis of the recorded signals suggested that despite the noise generated by the user's motion, it was still possible to detect the P300 in the signals in each of the three conditions. This opens new perspective of applications using a wearable P300-based BCI as input device, e.g., for entertainment and video games.
Effect of dietary supplementation of thymol, synbiotic and their combination on performance, egg quality and serum metabolic profile of Hy-Line Brown hens

Abstract An experiment was conducted to determine the effects of dietary supplementation of thymol, synbiotic (Biomin, IMBO) and their combination in laying hen diets on laying performance, egg quality and serum metabolic profile from 24 to 36 weeks of age. Treatment groups were fed on a control diet, the control diet supplemented with thymol (250 mg/kg), the control diet supplemented with synbiotic (250 mg/kg) or the control diet supplemented with a combination of thymol (250 mg/kg) and synbiotic (250 mg/kg). Supplementation of thymol and synbiotic, separately as well as combined, improved egg weight, egg production, egg mass and feed conversion ratio from 24 to 36 weeks of age. The eggs obtained from thymol, synbiotic or their combination treatments displayed higher values of shell thickness, Haugh unit and shell percentage compared to the control. Serum cholesterol significantly decreased in the single or combined form of thymol and synbiotic supplementation treatments.
An exploration of the relationship between two measures of children’s participation

Abstract Purpose: To investigate the relationship between the Children Participation Questionnaire and the Children’s Assessment of Participation with Hands. Materials and methods: Two researchers classified the item contents independently using the International Classification of Functioning, Disability and Health-Child and Youth version as a guide. Parents of 51 children with intellectual and developmental disabilities completed both measures within one month. Results: The linking results indicated that the two participation measures covered a broad range of life domains, which corresponded well to the conceptually matched Activities and Participation categories/chapters of the International Classification of Functioning, Disability and Health-Child and Youth version. A significant moderate link (Spearman’s ρ coefficient = 0.68, p < 0.01) between the two measures was found, and all of their matched domains (except for the educational domain) also revealed small to moderate associations (ρ = 0.47–0.71, p < 0.01). The paired items that asked identical or similar life situations between the two measures demonstrated varying correlation levels. Conclusion: This study provides evidence for the content and construct validity of the two children’s participation measures. The findings also offer important insights about the similarities and differences between the two measures. This knowledge will assist clinicians in selecting outcome measures. Implications for rehabilitation The Children Participation Questionnaire and Children’s Assessment of Participation with Hands demonstrate good content and convergent validity. The two measures capture distinctive aspects and/or degrees of children’s participation. Clinicians and researchers need to be aware of the similarities and differences between the two measures before selecting outcome measure.