Miracle-dz/UniFAR-FTUs huggingface dataset:
Microfluidics-Assisted Fabrication of Gelatin-Silica Core–Shell Microgels for Injectable Tissue Constructs

Microfabrication technology provides a highly versatile platform for engineering hydrogels used in biomedical applications with high-resolution control and injectability. Herein, we present a strategy of microfluidics-assisted fabrication photo-cross-linkable gelatin microgels, coupled with providing protective silica hydrogel layer on the microgel surface to ultimately generate gelatin-silica core–shell microgels for applications as in vitro cell culture platform and injectable tissue constructs. A microfluidic device having flow-focusing channel geometry was utilized to generate droplets containing methacrylated gelatin (GelMA), followed by a photo-cross-linking step to synthesize GelMA microgels. The size of the microgels could easily be controlled by varying the ratio of flow rates of aqueous and oil phases. Then, the GelMA microgels were used as in vitro cell culture platform to grow cardiac side population cells on the microgel surface. The cells readily adhered on the microgel surface and proliferated over time while maintaining high viability (∼90%). The cells on the microgels were also able to migrate to their surrounding area. In addition, the microgels eventually degraded over time. These results demonstrate that cell-seeded GelMA microgels have a great potential as injectable tissue constructs. Furthermore, we demonstrated that coating the cells on GelMA microgels with biocompatible and biodegradable silica hydrogels via sol–gel method provided significant protection against oxidative stress which is often encountered during and after injection into host tissues, and detrimental to the cells. Overall, the microfluidic approach to generate cell-adhesive microgel core, coupled with silica hydrogels as a protective shell, will be highly useful as a cell culture platform to generate a wide range of injectable tissue constructs.
Complete Open Loop Control of Hysteretic, Creeped, and Oscillating Piezoelectric Cantilevers

The feedforward compensation of nonlinearities, i.e., hysteresis and creep, and unwanted vibrations in micromanipulators is presented in this paper. The aim is to improve the general performances of piezocantilevers dedicated to micromanipulation/microassembly tasks. While hysteresis is attenuated using the Prandtl-Ishlinskii inverse model, a new method is proposed to decrease the creep phenomenon. As no model inversion is used, the proposed method is simple and easy to implement. Finally, we employ an input shaping technique to reduce the vibration of the piezocantilevers. The experimental results show the efficiency of the feedforward techniques and their convenience to the micromanipulation/microassembly requirements.
Accuracy and interrater reliability of paediatric emergency department triage

To determine the accuracy and reliability of triage of children in public hospital EDs using the Australasian Triage Scale (ATS). This is the first study to examine these issues in paediatric triage following the 2007 development of the Emergency Triage Education Kit (ETEK) to foster accurate and consistent application of the ATS.
Targeted Gene Disruption Reveals an Adhesin Indispensable for Pathogenicity of Blastomyces dermatitidis

Systemic fungal infections are becoming more common and difficult to treat, yet the pathogenesis of these infectious diseases remains poorly understood. In many cases, pathogenicity can be attributed to the ability of the fungi to adhere to target tissues, but the lack of tractable genetic systems has limited progress in understanding and interfering with the offending fungal products. In Blastomyces dermatitidis, the agent of blastomycosis, a respiratory and disseminated mycosis of people and animals worldwide, expression of the putative adhesin encoded by the WI-1 gene was investigated as a possible virulence factor. DNA-mediated gene transfer was used to disrupt the WI-1 locus by allelic replacement, resulting in impaired binding and entry of yeasts into macrophages, loss of adherence to lung tissue, and abolishment of virulence in mice; each of these properties was fully restored after reconstitution of WI-1 by means of gene transfer. These findings establish the pivotal role of WI-1 in adherence and virulence of B. dermatitidis yeasts. To our knowledge, they offer the first example of a genetically proven virulence determinant among systemic dimorphic fungi, and underscore the value of reverse genetics for studies of pathogenesis in these organisms.
Doping dependence of the redistribution of optical spectral weight in Bi 2 Sr 2 CaCu 2 O 8+δ

We present the ab-plane optical conductivity of four single crystals of ${\mathrm{Bi}}_{2}{\mathrm{Sr}}_{2}{\mathrm{CaCu}}_{2}{\mathrm{O}}_{8+\ensuremath{\delta}}\phantom{\rule{0.3em}{0ex}}(\mathrm{Bi}2212)$ with different carrier doping levels from the strongly underdoped to the strongly overdoped range with ${T}_{c}=66$, 88, 77, and $67\phantom{\rule{0.3em}{0ex}}\mathrm{K}$, respectively. We focus on the redistribution of the low frequency optical spectral weight (SW) in the superconducting and normal states. The temperature dependence of the low-frequency spectral weight in the normal state is significantly stronger in the overdoped regime. In agreement with other studies, the superconducting order is marked by an increase of the low frequency SW for low doping, while the SW decreases for the highly overdoped sample. The effect crosses through zero at a doping concentration $\ensuremath{\delta}=0.19$ which is slightly to the right of the maximum of the superconducting dome. This sign change is not reproduced by the BCS model calculations, assuming the electron-momentum dispersion known from ARPES data. Recent cluster dynamical mean field theory calculations based on the Hubbard and $t\text{\ensuremath{-}}J$ models, agree in several relevant respects with the experimental data.
Prognostic significance of cyclooxygenase-2 expression in patients with hepatocellular carcinoma: a meta-analysis

Introduction Cyclooxygenase-2 (COX-2) is believed to be an important enzyme in the carcinogenesis of hepatocellular carcinoma (HCC). However, it is still controversial whether COX-2 expression can be regarded as a prognostic factor for HCC patients. We performed a systematic review and meta-analysis of studies assessing the clinical and prognostic significance of COX-2 expression in HCC. Material and methods Identification and review of publications assessing clinical or prognostic significance of COX-2 expression in HCC until November 1, 2014. A meta-analysis was performed to clarify the association between COX-2 expression and clinical outcomes. Results A total of 11 publications met the criteria and included 943 cases. Analysis of these data showed that COX-2 expression was not significantly correlated with capsular formation (OR = 0.84, 95% confidence interval (CI): 0.46–1.55, p = 0.58), tumor TNM stage (OR = 0.73, 95% CI: 0.23–2.33, p = 0.59), vascular invasion (OR = 1.04, 95% CI: 0.25–4.35, p = 0.96), tumor size (OR = 0.78, 95% CI: 0.21–2.86, p = 0.71), or tumor differentiation degree (OR = 1.08, 95% CI: 0.42–2.79, p = 0.87). However, in the identified studies, COX-2 expression was strongly associated with high alpha-fetoprotein level (OR = 1.83, 95% CI: 1.01–3.33, p = 0.05), HBsAg status (OR = 1.85, 95% CI: 1.13–3.03, p = 0.01), decreased overall survival (relative risk (RR): 1.54, 95% CI: 1.18–2.02, p = 0.001) and decreased disease-free survival (RR = 1.49, 95% CI: 1.22–1.81, p < 0.001). Conclusions This meta-analysis shows that COX-2 expression in HCC is associated with decreased overall and disease-free survival and thus marks a worse prognosis. Nevertheless, more large sample and well-designed studies are warranted to confirm this finding.
Mammalian Expression of Virus-Like Particles for Advanced Mimicry of Authentic Influenza Virus

Background Influenza A viruses are major human and animal pathogens with huge economic and societal impact from illness, hospitalizations, and deaths. Virus-like particles (VLPs) of influenza virus have been suggested as a vaccine candidate offering improved safety and efficacy. To develop this concept further, we established a flexible platform to efficiently generate different subtypes of mammalian-expressed influenza VLPs. Here we demonstrate that these mammalian VLPs strongly resemble the authentic viruses in structure, particle size and composition of host factors, and even glycosylation of viral antigens. Methodology/Principal Findings In this study, a mammalian VLP system was established by stable co-expression of four influenza structural proteins (HA, NA, M1, and M2) in a Vero cell line. By replacing the surface glycoproteins of HA and NA, we converted the H3N2-VLP subtype to H5N1-VLP. After centrifugation purification of conditioned media, the particle morphologies, average sizes, and hemagglutination abilities of secreted VLPs were characterized, and the VLP constituents were identified by LC/MS/MS. Protease protection assays demonstrated that specific cellular proteins that co-purified with influenza virions were integrated into mammalian VLPs. The glycosylation profiles of mammalian VLPs as revealed by deglycosylation assays were similar to that of progeny viruses produced from Vero cells. Vaccination of mice with 2.5 µg and above of H5N1-VLP elicited H5-specific IgG1 antibodies and resulted in full protection against lethal infection with homologous virus. These results provide compelling evidence that mammalian VLPs closely emulate the exterior of authentic virus particles not only in antigen presentation but also in biological properties and should provide promising vaccine candidates. Conclusions/Significance This flexible mammalian influenza VLP system offers a superior alternative to the conventional reverse genetic vaccine platform without concerns over inadequate presentation of immune antigens or limitations imposed by the manipulation of real viruses.
The geometry of concurrent interaction: Handling multiple ports by way of multiple tokens

We introduce a geometry of interaction model for Mazza's multiport interaction combinators, a graph-theoretic formalism which is able to faithfully capture concurrent computation as embodied by process algebras like the π-calculus. The introduced model is based on token machines in which not one but multiple tokens are allowed to traverse the underlying net at the same time. We prove soundness and adequacy of the introduced model. The former is proved as a simulation result between the token machines one obtains along any reduction sequence. The latter is obtained by a fine analysis of convergence, both in nets and in token machines.
Reaction between MnO-SiO2-FeO Inclusion and Steel Matrix Deoxidized by Si and Mn at Different Heat Treatment Temperatures

In order to clarify the effect of heat-treatment temperature on the reaction between solid steel and inclusions, in this study, the reaction between Fe-Mn-Si alloy and MnO-SiO2-FeO oxide during heat-treatment at 1373 K, 1473 K and 1573 K were investigated using diffusion couple method, respectively. The results indicate that the width of the particle-precipitated zone (PPZ) and the Mndepleted zone (MDZ) increased with increasing heat-treatment temperature from 1373 K to 1473 K. However, compared with the experimental results at 1473 K, the precipitated particles in the alloy were significantly coarsened and the width of the PPZ decreased at 1573 K. In addition, with increasing heat-treatment time at 1573 K, the composition of the oxide changed from 2MnO·SiO2 to MnO·SiO2 and the manganese and silicon in the oxide obviously diffused into the alloy, resulting in a significant increase of Mn and Si contents in the alloy near the interface.
Bias and Reciprocity in Online Reviews: Evidence From Field Experiments on Airbnb

Reviews and other evaluations are used by consumers to decide what goods to buy and by firms to choose whom to trade with, hire, or promote. However, because potential reviewers are not compensated for submitting reviews and may have reasons to omit relevant information in their reviews, reviews may be biased. We use the setting of Airbnb to study the determinants of reviewing behavior, the extent to which reviews are biased, and whether changes in the design of reputation systems can reduce that bias. We find that reviews on Airbnb are generally informative and 97% of guests privately report having positive experiences. Using two field experiments intended to reduce bias, we show that non-reviewers tend to have worse experiences than reviewers and that strategic reviewing behavior occurred on the site, although the aggregate effect of the strategic behavior was relatively small. We use a quantitative exercise to show that the mechanisms for bias that we document decrease the rate of reviews with negative text and a non-recommendation by just . 86 percentage points. Lastly, we discuss how online marketplaces can design more informative review systems.
High Speed Multiple Valued Logic Full Adder Using Carbon Nano Tube Field Effect Transistor

High speed Full-Adder (FA) module is a critical element in designing high performance arithmetic circuits. In this paper, we propose a new high speed multiple-valued logic FA module. The proposed FA is constructed by 14 transistors and 3 capacitors, using carbon nano-tube field effect transistor (CNFET) technology. Furthermore, our proposed technique has been examined in different voltages (i.e., 0.65v and 0.9v). The observed results reveal power consumption and power delay product (PDP) improvements compared to existing FA counterparts
Tourette syndrome deep brain stimulation: A review and updated recommendations

Deep brain stimulation (DBS) may improve disabling tics in severely affected medication and behaviorally resistant Tourette syndrome (TS). Here we review all reported cases of TS DBS and provide updated recommendations for selection, assessment, and management of potential TS DBS cases based on the literature and implantation experience. Candidates should have a Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM V) diagnosis of TS with severe motor and vocal tics, which despite exhaustive medical and behavioral treatment trials result in significant impairment. Deep brain stimulation should be offered to patients only by experienced DBS centers after evaluation by a multidisciplinary team. Rigorous preoperative and postoperative outcome measures of tics and associated comorbidities should be used. Tics and comorbid neuropsychiatric conditions should be optimally treated per current expert standards, and tics should be the major cause of disability. Psychogenic tics, embellishment, and malingering should be recognized and addressed. We have removed the previously suggested 25‐year‐old age limit, with the specification that a multidisciplinary team approach for screening is employed. A local ethics committee or institutional review board should be consulted for consideration of cases involving persons younger than 18 years of age, as well as in cases with urgent indications. Tourette syndrome patients represent a unique and complex population, and studies reveal a higher risk for post‐DBS complications. Successes and failures have been reported for multiple brain targets; however, the optimal surgical approach remains unknown. Tourette syndrome DBS, though still evolving, is a promising approach for a subset of medication refractory and severely affected patients. © 2014 International Parkinson and Movement Disorder Society
{{citation needed}}: Filling in Wikipedia's Citation Shaped Holes

Wikipedia authors cite external references to support claims made in their articles in order to increase their validity. A large number of claims, however, do not have supporting citations, putting them in question. In this paper, we describe a study in which we attempt to retrieve relevant citations for claims using a variety of information retrieval algorithms. These algorithms are inspired by bibliometric and altmetric insights that exploit readership data from Mendeley’s community and rerank results using a Bradfordising approach. The results of the small scale study indicate that both of these approaches can improve upon basic keyword-based search, typically used in digital libraries, in order to return relevant documents for unsupported claims.
The Accuracy of Spot Sign in Predicting Hematoma Expansion after Intracerebral Hemorrhage: A Systematic Review and Meta-Analysis

Purpose The role of spot sign on computed tomography angiography (CTA) for predicting hematoma expansion (HE) after primary intracerebral hemorrhage (ICH) has been the focus of many studies. Our study sought to evaluate the predictive accuracy of spot signs for HE in a meta-analytic approach. Materials and Methods The database of Pubmed, Embase, and the Cochrane Library were searched for eligible studies. Researches were included if they reported data on HE in primary ICH patients, assessed by spot sign on first-pass CTA. Studies with additional data of second-pass CTA, post-contrast CT (PCCT) and CT perfusion (CTP) were also included. Results 18 studies were pooled into the meta-analysis, including 14 studies of first-pass CTA, and 7 studies of combined CT modalities. In evaluating the accuracy of spot sign for predicting HE, studies of first-pass CTA showed that the sensitivity was 53% (95% CI, 49%–57%) with a specificity of 88% (95% CI, 86%–89%). The pooled positive likelihood ratio (PLR) was 4.70 (95% CI, 3.28–6.74) and the negative likelihood ratio (NLR) was 0.44 (95% CI, 0.34–0.58). For studies of combined CT modalities, the sensitivity was 73% (95% CI, 67%–79%) with a specificity of 88% (95% CI, 86%–90%). The aggregated PLR was 6.76 (95% CI, 3.70–12.34) and the overall NLR was 0.17 (95% CI 0.06–0.48). Conclusions Spot signs appeared to be a reliable imaging biomarker for HE. The additional detection of delayed spot sign was helpful in improving the predictive accuracy of early spot signs. Awareness of our results may impact the primary ICH care by providing supportive evidence for the use of combined CT modalities in detecting spot signs.
Modelling of thermal stresses in bearing steel structure generated by electrical current impulses

This work is the study of one particular candidate for white etching crack (WEC) initiation mechanism in wind turbine gearbox bearings: discharge current impulses flowing through bearing steel with associated thermal stresses and material fatigue. Using data/results from previously published works, the authors develop a series of models that are utilized to simulate these processes under various conditions/local microstructure configurations, as well as to verify the results of the previous numerical studies. Presented models show that the resulting stresses are several orders of magnitude below the fatigue limit/yield strength for the parameters used herein. Results and analysis of models provided by Scepanskis, M. et al. also indicate that certain effects predicted in their previous work resulted from a physically unfounded assumption about material thermodynamic properties and numerical model implementation issues.
δ‐Catenin/NPRAP: A new member of the glycogen synthase kinase‐3β signaling complex that promotes β‐catenin turnover in neurons

Through a multiprotein complex, glycogen synthase kinase‐3β (GSK‐3β) phosphorylates and destabilizes β‐catenin, an important signaling event for neuronal growth and proper synaptic function. δ‐Catenin, or NPRAP (CTNND2), is a neural enriched member of the β‐catenin superfamily and is also known to modulate neurite outgrowth and synaptic activity. In this study, we investigated the possibility that δ‐catenin expression is also affected by GSK‐3β signaling and participates in the molecular complex regulating β‐catenin turnover in neurons. Immunofluorescent light microscopy revealed colocalization of δ‐catenin with members of the molecular destruction complex: GSK‐3β, β‐catenin, and adenomatous polyposis coli proteins in rat primary neurons. GSK‐3β formed a complex with δ‐catenin, and its inhibition resulted in increased δ‐catenin and β‐catenin expression levels. LY294002 and amyloid peptide, known activators of GSK‐3β signaling, reduced δ‐catenin expression levels. Furthermore, δ‐catenin immunoreactivity increased and protein turnover decreased when neurons were treated with proteasome inhibitors, suggesting that the stability of δ‐catenin, like that of β‐catenin, is regulated by proteasome‐mediated degradation. Coimmunoprecipitation experiments showed that δ‐catenin overexpression promoted GSK‐3β and β‐catenin interactions. Primary cortical neurons and PC12 cells expressing δ‐catenin treated with proteasome inhibitors showed increased ubiquitinated β‐catenin forms. Consistent with the hypothesis that δ‐catenin promotes the interaction of the destruction complex molecules, cycloheximide treatment of cells overexpressing δ‐catenin showed enhanced β‐catenin turnover. These studies identify δ‐catenin as a new member of the GSK‐3β signaling pathway and further suggest that δ‐catenin is potentially involved in facilitating the interaction, ubiquitination, and subsequent turnover of β‐catenin in neuronal cells. © 2010 Wiley‐Liss, Inc.
Body fat influences departure from stopover sites in migratory birds: evidence from whole-island telemetry

Migration remains one of the great mysteries of animal life. Small migratory birds rely on refuelling stopovers after crossing ecological barriers such as deserts or seas. Previous studies have suggested that fuel reserves may determine stopover duration but this hypothesis could not be tested because of methodological limitations. Here, we provide evidence that subcutaneous fat stores determine stopover duration by measuring the permanence of migratory garden warblers (Sylvia borin) on a small Mediterranean island during spring migration with telemetry methods. Garden warblers with large amounts of fat stores departed the island significantly sooner than lean birds. All except one fat bird left the island on the same evening after capture, with a mean total stopover estimate of 8.8 hours. In contrast, the mean estimated total stopover duration of lean birds was 41.3 hours. To our knowledge, this is the first study that measures the true minimum stopover duration of a songbird during migration.