Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of ouabain on fluid transport and electrical properties of Necturus gallbladder. Evidence in favor of a neutral basolateral sodium transport mechanism

Net fluid transport (Jv) and electrical properties of the cell membranes and paracellular pathway of Necturus gallbladder epithelium were studied before and after the addition of ouabain (10(-4) M) to the serosal bathing medium. The glycoside inhibited Jv by 70% in 15 min and by 100% in 30 min. In contrast, the potentials across both cell membranes did not decrease significantly until 20 min of exposure to ouabain. At 30 min, the basolateral membrane potential (Vcs) fell only by ca 7 mV. If basolateral Na transport were electrogenic, with a coupling ratio (Na:K) of 3:2, the reductions of Vcs at 15 and 30 min should be 12--15 and 17--21 mV, respectively. Thus, we conclude that the mechanism of Na transport from the cells to the serosal bathing solution is not electrogenic under normal transport conditions. The slow depolarization observed in ouabain is caused by a fall of intracellular K concentration, and by a decrease in basolateral cell membrane K permeability. Prolonged exposure to ouabain results also in an increase in paracellular K selectivity, with no change of P Na/P Cl.
The Role of Orbitofrontal Cortex in Processing Empathy Stories in 4- to 8-Year-Old Children

This study investigates the neuronal correlates of empathic processing in children aged 4–8 years, an age range discussed to be crucial for the development of empathy. Empathy, defined as the ability to understand and share another person's inner life, consists of two components: affective (emotion-sharing) and cognitive empathy (Theory of Mind). We examined the hemodynamic responses of preschool and school children (N = 48), while they processed verbal (auditory) and non-verbal (cartoons) empathy stories in a passive following paradigm, using functional Near-Infrared Spectroscopy. To control for the two types of empathy, children were presented blocks of stories eliciting either affective or cognitive empathy, or neutral scenes which relied on the understanding of physical causalities. By contrasting the activations of the younger and older children, we expected to observe developmental changes in brain activations when children process stories eliciting empathy in either stimulus modality toward a greater involvement of anterior frontal brain regions. Our results indicate that children's processing of stories eliciting affective and cognitive empathy is associated with medial and bilateral orbitofrontal cortex (OFC) activation. In contrast to what is known from studies using adult participants, no additional recruitment of posterior brain regions was observed, often associated with the processing of stories eliciting empathy. Developmental changes were found only for stories eliciting affective empathy with increased activation, in older children, in medial OFC, left inferior frontal gyrus, and the left dorsolateral prefrontal cortex. Activations for the two modalities differ only little, with non-verbal presentation of the stimuli having a greater impact on empathy processing in children, showing more similarities to adult processing than the verbal one. This might be caused by the fact that non-verbal processing develops earlier in life and is more familiar.
High prevalence of eating disorders in narcolepsy with cataplexy: a case-control study.

STUDY OBJECTIVES To study the prevalence of and symptoms of eating disorders in patients with narcolepsy. DESIGN We performed a case-control study comparing symptoms of eating disorders in patients with narcolepsy versus healthy population controls, using the Schedules for Clinical Assessment in Neuropsychiatry (SCAN 2.1). To study whether an increased body mass index (BMI) could be responsible for symptoms of an eating disorder, we also compared patients with BMI-matched controls, using the SCAN as well as the Eating Disorder Examination-Questionnaire. SETTING University hospital. PATIENTS AND PARTICIPANTS Patients with narcolepsy/cataplexy (n = 60) were recruited from specialized sleep centers. Healthy controls (n = 120) were drawn from a population study previously performed in the Netherlands. Separately, 32 BMI-matched controls were recruited. INTERVENTIONS N/A. MEASUREMENTS AND RESULTS In total, 23.3% of the patients fulfilled the criteria for a clinical eating disorder, as opposed to none of the control subjects. Most of these were classified as Eating Disorder-Not Otherwise Specified, with an incomplete form of binge eating disorder. On the symptom level, half of the patients reported a persistent craving for food, as well as binge eating. Twenty-five percent of patients even reported binging twice a week or more often. When compared with BMI-matched controls, the significant increases persisted in symptoms of eating disorders among patients with narcolepsy. Except for a higher level of interference in daily activities due to eating problems in patients using antidepressants, medication use did not influence our findings. CONCLUSIONS The majority of patients with narcolepsy experience a number of symptoms of eating disorders, with an irresistible craving for food and binge eating as the most prominent features. Eating disorder symptomatology interfered with daily activities. These findings justify more attention for eating disorders in the treatment of patients with narcolepsy.
Emergence of the M Phenotype of Erythromycin-Resistant Pneumococci in South Africa

Resistance to erythromycin in pneumococci has been observed since 1967 (1) and was first reported in South African multiresistant pneumococcal strains in 1978 (2). Until recently, the only mechanism described for resistance to erythromycin in the pneumococcus was the N6methylation of a specific adenine residue (A2058) in 23S rRNA, which resulted in reduced affinity between the antibiotic and the ribosome (3,4). This resistance is associated with the gene ermAM (5), first described in Streptococcus sanguis (6). Since then, other mechanisms of erythromycin resistance in the pneumococcus have been reported. In fact, most resistance in the United States appears to be due to efflux of the antibiotic from the cell, associated with the gene mefE (7,8). While ermAM confers coresistance to most macrolides, lincosamides, and streptogramin B antibiotics (resulting in the so-called MLS phenotype) (3,9), mefE confers resistance only to the 14and 15-membered macrolides (resulting in the M phenotype) (7,8). We report the emergence of M-phenotype erythromycin resistance in South African blood and cerebrospinal fluid (CSF) pneumococcal isolates from 1987 to 1996. The South African Institute for Medical Research (SAIMR), Johannesburg, South Africa, regularly receives all pneumococcal isolates from participating laboratories in eight of the nine provinces of South Africa. We examined all erythromycin-resistant blood and CSF isolates received by SAIMR from 1987 to 1996. Erythromycin-, clindamycin-, and penicillinresistance phenotypes were determined by using disk diffusion assays (erythromycin, 15μg/disk, clindamycin, 2 μg/disk, oxacillin, 1 μg/disk) on 5% horse blood agar plates (Mueller-Hinton base) after overnight growth at 37oC under aerobic conditions. Strains showing resistance (zone diameters ≤ 20mm for erythromycin, ≤ 18mm for clindamycin, and <20mm for oxacillin) on the disk diffusion plates were tested by the agar dilution method to obtain MICs according to the National Committee for Clinical Laboratory Standards guidelines (10). We evaluated (by the chi-square test) increases in the prevalence of erythromycin resistance and the incidence of resistance to erythromycin and susceptibility to clindamycin, which represents the M phenotype. The M phenotype increased significantly from 1987 to 1991 compared with 1992 to 1996 in Emergence of the M Phenotype of Erythromycin-Resistant Pneumococci in South Africa
Control of airway function during and after exercise in asthmatics.

Control of airway function during and after exercise in asthmatics. Med. Sci. Sports Exerc. , Vol. 31, No. 1 (Suppl.), pp. S4-S11, 1999. In asthmatics, airway function can be quite variable during exercise depending on the level of exercise intensity, the duration of exercise, and whether the exercise is at constant load or variable in intensity. Airway diameter can be affected by activity of parasympathetic and sympathetic nerves, by systemic mediators such as catecholamines, and by local mediators such as histamine or leukotrienes. Asthmatic airways are populated with more inflammatory cells than normal airways, and bronchoconstrictor mediator release from these cells is probably caused by drying of the mucosa during and after periods of increased ventilation. There are a few bronchodilating mediators present in both asthmatic and normal airways that could protect against this bronchoconstriction, including prostaglandin PGE2 and nitric oxide. Although it is clear that many of the inflammatory mediators play a role in causing bronchoconstriction after exercise, the role of either bronchoconstrictor or bronchodilator mediators in controlling airway function during exercise has yet to be resolved. In addition, the mechanical interaction between lung parenchyma and airways may provide a bronchodilating influence. In conclusion, the variability in airway function during exercise in asthmatics could be caused by balance among various bronchodilator and bronchoconstrictor mediators, but it may also reflect a mechanical effect of varying levels of ventilation.
Memory of Natural Killer Cells: A New Chance against Mycobacterium tuberculosis?

Natural killer (NK) cells are lymphocytes of the innate immune system, which play an important role in the initial defense against a wide variety of pathogens, including viruses and intracellular bacteria. NK cells produce cytokines that enhance immune responses directed toward pathogens and also exert cytotoxic activity against infected cells, thereby eliminating the reservoir of infection. Their role in defense against Mycobacterium tuberculosis (Mtb) has been recently studied, and there is increasing evidence that highlight the importance of NK cell function during pulmonary tuberculosis (PTB), especially in the absence of optimal T-cell responses. Additionally, in the last years, it has been observed that NK cells mediate secondary responses against antigens to which they were previously exposed, an ability classically attributed to lymphocytes of the adaptive branch of immunity. This phenomenon, called “innate memory,” could have important implications in the efforts to develop therapies and vaccines to improve the initial phases of immune reactions against different microorganisms, especially those to which there is not yet available vaccines to prevent infection, as is the case for tuberculosis. Therefore, the possibility of inducing memory-like NK cells ready to act prior to contact with Mtb or during the earliest stages of infection becomes quite interesting. However, our understanding of the mechanisms of innate memory remains incomplete. Here, we review recent literature about the mechanisms involved in the formation and maintenance of NK cell memory and the role of these cells in the immune response during tuberculosis. Finally, we discuss if the current evidence is sufficient to substantiate that NK cells exert more rapid and robust secondary responses after consecutive encounters with Mtb.
The nuclear receptor REV-ERBα mediates circadian regulation of innate immunity through selective regulation of inflammatory cytokines

Diurnal variation in inflammatory and immune function is evident in the physiology and pathology of humans and animals, but molecular mechanisms and mediating cell types that provide this gating remain unknown. By screening cytokine responses in mice to endotoxin challenge at different times of day, we reveal that the magnitude of response exhibited pronounced temporal dependence, yet only within a subset of proinflammatory cytokines. Disruption of the circadian clockwork in macrophages (primary effector cells of the innate immune system) by conditional targeting of a key clock gene (bmal1) removed all temporal gating of endotoxin-induced cytokine response in cultured cells and in vivo. Loss of circadian gating was coincident with suppressed rev-erbα expression, implicating this nuclear receptor as a potential link between the clock and inflammatory pathways. This finding was confirmed in vivo and in vitro through genetic and pharmacological modulation of REV-ERBα activity. Circadian gating of endotoxin response was lost in rev-erbα−/− mice and in cultured macrophages from these animals, despite maintenance of circadian rhythmicity within these cells. Using human macrophages, which show circadian clock gene oscillations and rhythmic endotoxin responses, we demonstrate that administration of a synthetic REV-ERB ligand, or genetic knockdown of rev-erbα expression, is effective at modulating the production and release of the proinflammatory cytokine IL-6. This work demonstrates that the macrophage clockwork provides temporal gating of systemic responses to endotoxin, and identifies REV-ERBα as the key link between the clock and immune function. REV-ERBα may therefore represent a unique therapeutic target in human inflammatory disease.
Non-word repetition in 2-year-olds: Replication of an adapted paradigm and a useful methodological extension

Abstract Accurate non-word repetition (NWR) has been largely attributed to phonological memory, although the task involves other processes including speech production, which may confound results in toddlers with developing speech production abilities. This study is based on Hoff, Core and Bridges’ adapted NWR task, which includes a real-word repetition (RWR) condition. We tested 86 typically developing 2-year-olds and found relationships between NWR and both receptive and expressive vocabulary using a novel measure that controls for speech production by comparing contextually matched targets in RWR. Post hoc analyses demonstrated the influence of lexical and sublexical factors in repetition tasks. Overall, results illustrate the importance of controlling for speech production differences in young children and support a useful methodological approach for testing NWR.
Linear capacity scaling in wireless networks: Beyond physical limits?

We investigate the role of cooperation in wireless networks subject to a spatial degrees of freedom limitation. To address the worst case scenario, we consider a free-space line-of-sight type environment with no scattering and no fading. We identify three qualitatively different operating regimes that are determined by how the area of the network A, normalized with respect to the wavelength ¿, compares to the number of users n. In networks with ¿A/¿ ¿ ¿n, the limitation in spatial degrees of freedom does not allow to achieve a capacity scaling better than ¿n and this performance can be readily achieved by multi-hopping. This result has been recently shown in [7]. However, for networks with ¿A/¿ > ¿n the number of available degrees of freedom is min (n, ¿A/¿), larger that what can be achieved by multi-hopping. We show that the optimal capacity scaling in this regime is achieved by hierarchical cooperation. In particular, in networks with ¿A/¿ > n, hierarchical cooperation can achieve linear scaling.
Constraints on Galactic intermediate mass black holes

Intermediate Mass Black Holes (IMBHs; 10 1.3−5 M⊙) are thought to form as relics of Population III stars or from the runaway collapse of stars in young clusters; their number and very existence are uncertain. We ran N-body simulations of Galactic IMBHs, modelling them as a halo population distributed according to a Navarro, Frenk & White (NFW) or a more concentrated Diemand, Madau & Moore (DMM) density profile. As IMBHs pass through Galactic molecular/atomic hydrogen regions, they accrete gas, thus becoming X-ray sources. We constrain the density of Galactic IMBHs, •, by comparing the distribution of simulated X-ray sources with the observed one. From the null detections of Milky Way Ultra-Luminous X-ray sources, and from a comparison of simulations with unidentified sources in the IBIS/ISGRI catalogue we find a strong upper limit • � 10 −2 b(� 10 −1 b) for a DMM (NFW) profile, if IMBHs accrete via ADAF disks. Slightly stronger constraints (• � 10 −3 b for a DMM profile; • � 10 −2 b for a NFW profile) can be derived if IMBHs accrete with higher efficiency, such as by forming thin accretion disks. Although not very tight, such constraints are the most stringent ones derived so far in the literature.
Chronic Cerebrospinal Venous Insufficiency Is Not Associated with Multiple Sclerosis and Its Severity: A Blind-Verified Study

Background Chronic Cerebrospinal Venous Insufficiency (CCSVI) has been associated with multiple sclerosis (MS) with a risk ranging from as high as two-hundred-fold to a protective effect. However, not all studies were blinded, and the efficacy of blinding was never assessed. Objective To evaluate the association of CCSVI with MS in a cross-sectional blinded study and look for any association of CCSVI with the severity of MS. Methodology/Principal Findings The Echo-color Doppler examination was carried out in accordance with Zamboni’s five criteria in 68 consecutive MS patients and 68 healthy controls, matched by gender and age (±5 years). Four experienced neurosonologists, blinded to the status of cases and controls, performed the study and were then asked to guess the status (case or control) of each participant. The number of positive CCSVI criteria was similar in the two groups. CCSVI, defined as the presence of two or more criteria, was detected in 21 cases (30.9%) and 23 controls (33.8%), with an OR of 0.9 (95%CL = 0.4–1.8, p = 0.71). The prevalence of CCSVI was related to age in cases (OR increasing from 0.2 to 1.4), but not in controls. CCSVI positive (N = 21) and negative (N = 47) MS patients were similar in clinical type, age at disease onset, disability, and fatigue. Disease duration was longer (16.5±9.8 years) in CCSVI positive than negative patients (11.5±7.4; p = 0.04). The operators correctly guessed 34/68 cases (50%) and 45/68 controls (66%) (p = 0.06), indicating a different success of blinding. Conclusions/Significance CCSVI was not associated with MS itself, nor its severity. We cannot rule out the possibility that CCSVI is a consequence of MS or of aging. Blinding of sonographers is a key point in studying CCSVI and its verification should be a requisite of future studies.
Are Sparse Representations Rich Enough for Acoustic Modeling?

We propose a novel approach to acoustic modeling based on recent advances in sparse representations. The key idea in sparse coding is to compute a compressed local representation of a signal via an over-complete basis or dictionary that is learned in an unsupervised way. In this study, we compute the local representation on speech spectrogram as the raw “signal” and use it as the local sparse code to perform a standard phone classification task. A linear classifier is used that directly receives the coding space for making the classification decision. The simplicity of the linear classifier allows us to assess whether the sparse representations are sufficiently rich to serve as effective acoustic features for discriminating speech classes. Our experiments demonstrate competitive error rates when compared to other shallow approaches. An examination of the dictionary learned in sparse feature extraction demonstrates meaningful acoustic-phonetic properties that are captured by a collection of the dictionary entries.
Effects of follicular phase exercise on luteinizing hormone pulse characteristics in sedentary eumenorrhoeic women

OBJECTIVE Current studies reveal little regarding the Inception of exercise‐induced LH changes during physical training. This study aimed to assess the susceptibility of the hypothalamic–pituitary axis to the acute physical stress of exercise in untrained, physically inactive women. The acute effects of submaximal endurance exercise upon the pulsatile LH secretion in the follicular phase were compared with those accompanying leisurely strolling for a similar time period.
Research on CPU Workload Prediction and Balancing in Cloud Environment

Servers workload in the cloud environment should be balanced in order to achieve high efficiency and reduce resources consuming. One of the solutions is based on workload prediction, and design a proper load migration and balancing strategy. For the ease of discussion, we focus on CPU workload only in this paper. Specifically, considering the characteristics of workload, such as the strong correlation with time, we employ a time-series based two-step method to predict the CPU workload for both individual physical server and the cluster. Then, with the knowledge of the cluster workload, we design a strategy for workload migration and load balancing. Besides, we conduct extensive experiments to evaluate our method.
Intersection of two signalling pathways: extracellular nucleotides regulate pollen germination and pollen tube growth via nitric oxide

Plant and animal cells release or secrete ATP by various mechanisms, and this activity allows extracellular ATP to serve as a signalling molecule. Recent reports suggest that extracellular ATP induces plant responses ranging from increased cytosolic calcium to changes in auxin transport, xenobiotic resistance, pollen germination, and growth. Although calcium has been identified as a secondary messenger for the extracellular ATP signal, other parts of this signal transduction chain remain unknown. Increasing the extracellular concentration of ATPγS, a poorly-hydrolysable ATP analogue, inhibited both pollen germination and pollen tube elongation, while the addition of AMPS had no effect. Because pollen tube elongation is also sensitive to nitric oxide, this raised the possibility that a connection exists between the two pathways. Four approaches were used to test whether the germination and growth effects of extracellular ATPγS were transduced via nitric oxide. The results showed that increases in extracellular ATPγS induced increases in cellular nitric oxide, chemical agonists of the nitric oxide signalling pathway lowered the threshold of extracellular ATPγS that inhibits pollen germination, an antagonist of guanylate cyclase, which can inhibit some nitric oxide signalling pathways, blocked the ATPγS-induced inhibition of both pollen germination and pollen tube elongation, and the effects of applied ATPγS were blocked in nia1nia2 mutants, which have diminished NO production. The concurrence of these four data sets support the conclusion that the suppression of pollen germination and pollen tube elongation by extracellular nucleotides is mediated in part via the nitric oxide signalling pathway.