Miracle-dz/UniFAR-FTUs huggingface dataset:
Clonally related IgA‐ and IgE‐secreting plasma cells in a myeloma patient

Abstract: Objectives: The purpose of this work was to study the clonal relationship between the cells that secrete monoclonal proteins in an IgA/IgE double multiple myeloma patient. Double monoclonal gammopathy is a rare condition in which two types of monoclonal proteins can be found in the serum and/or urine of patients with multiple myeloma or gammopathy of undetermined significance. The study of the relationship between the cells expressing the different monoclonal proteins may provide insight in the pathogenesis of these disorders. Methods: The clonal relationship of the two tumoral plasma cell populations was examined by immunophenotyping and sequence analysis of the variable regions of the immunoglobulin heavy chain genes. Both immunoglobulin sequences were isolated from the bone marrow using a polymerase chain reaction (PCR)‐based cloning strategy. Rare isotype‐switch variants were detected by a myeloma‐specific PCR in combination with different isotype‐specific primers. An in vitro culture system, based on the activation of the CD40 molecule on the B cell, was used in order to isolate and expand myeloma‐related B cells from peripheral blood that could possibly be regarded as myeloma precursor cells. Results: The variable parts of the immunoglobulin heavy chains linked to either Cα or Cε were exactly the same, including the same somatic mutations. From the in vitro CD40 cultures B cells could be isolated that either expressed IgA or IgE with exactly the same variable immunoglobulin part as the myeloma clone. No pre‐switched IgM myeloma‐related B cells could be found. Conclusion: Both cell populations in this IgA/IgE myeloma patient shared a common clonal origin. No evidence for a pre‐switched IgM precursor myeloma cell was found in this patient.
In Vivo Validation of Custom-Designed Silicon-Based Microelectrode Arrays for Long-Term Neural Recording and Stimulation

We developed and validated silicon-based neural probes for neural stimulating and recording in long-term implantation in the brain. The probes combine the deep reactive ion etching process and mechanical shaping of their tip region, yielding a mechanically sturdy shank with a sharpened tip to reduce insertion force into the brain and spinal cord, particularly, with multiple shanks in the same array. The arrays' insertion forces have been quantified in vitro. Five consecutive chronically-implanted devices were fully functional from 3 to 18 months. The microelectrode sites were electroplated with iridium oxide, and the charge injection capacity measurements were performed both in vitro and after implantation in the adult feline brain. The functionality of the chronic array was validated by stimulating in the cochlear nucleus and recording the evoked neuronal activity in the central nucleus of the inferior colliculus. The arrays' recording quality has also been quantified in vivo with neuronal spike activity recorded up to 566 days after implantation. Histopathology evaluation of neurons and astrocytes using immunohistochemical stains indicated minimal alterations of tissue architecture after chronic implantation.
Long-Term Bank Balance Sheet Management: Estimation and Simulation of Risk-Factors

We propose a dynamic framework which encompasses the main risks in balance sheets of banks in an integrated fashion. Our contributions are fourfold: (1) solving a simple one-period model that describes the optimal bank policy under credit risk; (2) estimating the long-term stochastic processes underlying the risk factors in the balance sheet, taking into account the credit and interest rate cycles; (3) simulating several scenarios for interest rates and charge-offs; and (4) describing the equations that govern the evolution of the balance sheet in the long run. The models that we use address momentum and the interaction between different rates. Our results enable simulation of bank balance sheets over time given a bank’s lending strategy and provides a basis for an optimization model to determine bank asset–liability management strategy endogenously.
Convergence of the equilibrium prices in a family of financial models

Abstract. In this paper, we consider a family of complete or incomplete financial models such that the price processes of the financial assets converge in distribution to those in a limit model. Different authors pointed out that we do not have necessarily convergence of the arbitrage pricing intervals in that context. We prove here that we have very good convergence properties for the equilibrium pricing interval as defined by Bizid et al. (1998) in discrete time or Jouini and Napp (1999) in continuous time.
HCV-specific cytokine induction in monocytes of patients with different outcomes of hepatitis C.

AIM Cytokine release by macrophages critically determines the type of immune response to an antigen. Therefore, we studied hepatitis C virus (HCV)-specific induction of interleukins-1 beta, -10, -12 (IL-1 beta, IL-10, IL-12), and tumor necrosis factor-alpha (TNF-alpha) in monocytes. METHODS Intracellular cytokine expression was studied by flow cytometry in 23 patients with chronic hepatitis C, 14 anti-HCV seropositives without viremia and 11 controls after stimulation of peripheral blood mononuclear cells with recombinant core, NS3, NS4, NS5a and NS5b proteins. RESULTS Patients with HCV viremia revealed greater spontaneous expression of IL-1beta, TNF-alpha, and IL-10. Furthermore, greater than twofold higher IL-10 expression was induced by the HCV antigens in chronic hepatitis C than in the other two groups (P<0.05). In contrast, neither IL-12 nor TNF-alpha was induced preferentially. CONCLUSION In chronic hepatitis C antigen-specific cytokine induction in monocytes is apparently shifted towards predominant IL-10 induction - not counterbalanced by antiviral type 1 cytokines. This may contribute to persistent viral replication.
Resolving Translation Ambiguity using Non-parallel Bilingual Corpora

This paper presents an unsupervised method for choosing the correct translation of a word in context. It learns disambiguation information from nonparallel bilinguM corpora (preferably in the same domain) free from tagging. Our method combines two existing unsupervised disambiguation algorithms: a word sense disambiguation algorithm based on distributional clustering and a translation disambiguation algorithm using target language corpora. For the given word in context, the former algorithm identifies its meaning as one of a number of predefined usage classes derived by clustering a large amount of usages in the source language corpus. The latter algorithm is responsible for associating each usage class (i.e., cluster) with a target word that is most relevant to the usage. This paper also shows preliminary results of translation experiments.
Fetal growth in the offspring of epileptic women: results of an Italian multicentric cohort study

ABSTRACT‐ An historical cohort study of the association between maternal epilepsy, anticonvulsant drugs and fetal growth was carried out among 47 hospitals collaborating in the Italian Multicentric Registry on Birth Defects (IPIMC). Birth weight, head circumference and body length were studied in 164 babies of epileptic women and compared to 185 controls. Seventy‐nine epileptic women were treated by monotherapy, 59 by polytherapy and 26 took no anticonvulsant during pregnancy. An intrauterine growth retardation and a smaller head circumference was observed among babies of epileptic women. No effect was evident for body length. When specific anticonvulsant therapies were taken into account, only phenobarbital show ed an effect on birth weight and head circumference; a reduction in head circum ference was observed also in the babies of untreated epileptic women. The other antiepileptics (carbamazepine and valproic acid) showed no influence on the outcome considered. The observed effects on fetal growth can be interpreted as a result of an interaction between the effect of the maternal disease and that of the anticonvul‐sants.
Impact of the intra‐seasonal oscillation on tropical cyclone genesis over the western North Pacific

This study investigates the impact of the East Asian–western North Pacific (EAWNP) intra‐seasonal oscillation (ISO) on tropical cyclone (TC) genesis over the South China Sea (SCS) and the western North Pacific (WNP). The result indicates that the 20–70‐day oscillation (EAWNP‐ISO1) mainly modulates the frequency of TC genesis, while the 10–20‐day quasi‐biweekly oscillation (EAWNP‐ISO2) usually influences the genesis location. The impacts of ISO are not only on the individual TC genesis but also on the intra‐seasonal variation of TC activity.
CONTRIBUTION OF CIRCADIAN PHYSIOLOGY AND SLEEP HOMEOSTASIS TO AGE-RELATED CHANGES IN HUMAN SLEEP

The circadian pacemaker and sleep homeostasis play pivotal roles in vigilance state control. It has been hypothesized that age-related changes in the human circadian pacemaker, as well as sleep homeostatic mechanisms, contribute to the hallmarks of age-related changes in sleep, that is, earlier wake time and reduced sleep consolidation. Assessments of circadian parameters in healthy young (∼20–30 years old) and older people (∼65–75 years old)—in the absence of the confounding effects of sleep, changes in posture, and light exposure—have demonstrated that an earlier wake time in older people is accompanied by about a 1h advance of the rhythms of core body temperature and melatonin. In addition, older people wake up at an earlier circadian phase of the body temperature and plasma melatonin rhythm. The amplitude of the endogenous circadian component of the core body temperature rhythm assessed during constant routine and forced desynchrony protocols is reduced by 20–30% in older people. Recent assessments of the intrinsic period of the human circadian pacemaker in the absence of the confounding effects of light revealed no age-related reduction of this parameter in both sighted and blind individuals. Wake maintenance and sleep initiation are not markedly affected by age except that sleep latencies are longer in older people when sleep initiation is attempted in the early morning. In contrast, major age-related reductions in the consolidation and duration of sleep occur at all circadian phases. Sleep of older people is particularly disrupted when scheduled on the rising limb of the temperature rhythm, indicating that the sleep of older people is more susceptible to arousal signals genernpated by the circadian pacemaker. Sleep-homeostatic mechanisms, as assayed by the sleep-deprivation–induced increase of EEG slow-wave activity (SWA), are operative in older people, although during both baseline sleep and recovery sleep SWA in older people remains at lower levels. The internal circadian phase advance of awakening, as well as the age-related reduction in sleep consolidation, appears related to an age-related reduction in the promotion of sleep by the circadian pacemaker during the biological night in combination with a reduced homeostatic pressure for sleep. Early morning light exposure associated with this advance of awakening in older people could reinforce the advanced circadian phase. Quantification of the interaction between sleep homeostasis and circadian rhythmicity contributes to understanding age-related changes in sleep timing and quality. (Chronobiology International, 17(3), 285–311, 2000)
How policy on employee involvement in work reintegration can yield its opposite: employee experiences in a Canadian setting

Purpose: Canada has a long tradition of involving employee representatives in developing work reintegration policies and expects this to positively affect employee involvement to improve work reintegration success. The purpose of this study was to examine employee involvement in reintegration in a Canadian province as experienced by employees. Method: Fourteen semi-structured interviews were held with employees in a healthcare organization. The interview topic list was based on a review of local reintegration policy documents and literature. Interviews were transcribed verbatim and analysed using ethnographic methodology. Results Employees do not feel in control of their reintegration trajectory. In the phase of reporting sickness absence, they wrestle with a lack of understanding on how to report in sick. In the phase of reintegration planning and coordination, they hesitate to get involved in the organization of reintegration. In the phase of reintegration plan execution, employees encounter unfulfilled expectations on interventions. Conclusion: Employee involvement in the organization of reintegration makes them responsible for the development of reintegration trajectories. However, they consider themselves often incapable of completing this in practice. Moreover, employees experience that their contribution can boomerang on them. Implications for Rehabilitation It is not that employees are not able to think along or decide on their reintegration trajectory but rather they are expected to do so at times when they cannot oversee their illness and/or recovery trajectory. Settings out reintegration procedures that are inflexible in practice do not recognize that employee involvement in work reintegration trajectories can develop over time. The disability management professional has a central role in organizing and supporting employee involvement in work reintegration, however, the employees do not experience this is indeed happening.
Coping With The Conflicts Between Agriculture And Biodiversity Conservation In Kiko Town Council In The Western Parts Of Kibale National Park, Kabarole District

xi CHAPTER ONE . xi 1.0. INTRODUCTION. 13 1.1. Background of the study . 13 1.2 Statement of the problem . 17 1.3. Objectives of the study. 18 1.3. 1 Overall objective . 18 1.3. 2 Specific objective . 18 1.4. Hypotheses . 19 1.5. Significance of the study . 19 1.6. Limitations of the research . 19 1.7. Scope of the study. 20 1.7. 1. Geographical scope . 20 1.7. 2. Content scope . 20
A Lossless Embedded Compression Using Significant Bit Truncation for HD Video Coding

Increasing the image size of a video sequence aggravates the memory bandwidth problem of a video coding system. Despite many embedded compression (EC) algorithms proposed to overcome this problem, no lossless EC algorithm able to handle high-definition (HD) size video sequences has been proposed thus far. In this paper, a lossless EC algorithm for HD video sequences and related hardware architecture is proposed. The proposed algorithm consists of two steps. The first is a hierarchical prediction method based on pixel averaging and copying. The second step involves significant bit truncation (SBT) which encodes prediction errors in a group with the same number of bits so that the multiple prediction errors are decoded in a clock cycle. The theoretical lower bound of the compression ratio of the SBT coding was also derived. Experimental results have shown a 60% reduction of memory bandwidth on average. Hardware implementation results have shown that a throughput of 14.2 pixels/cycle can be achieved with 36 K gates, which is sufficient to handle HD-size video sequences in real time.
2 00 0 Modelling fluctuations of financial time series : from cascade process to stochastic volatility model

In this paper, we provide a simple, “generic” interpretation of multifractal scaling laws and multiplicative cascade process paradigms in terms of volatility correlations. We show that in this context 1/f power spectra, as recently observed in Ref. [20], naturally emerge. We then propose a simple solvable “stochastic volatility” model for return fluctuations. This model is able to reproduce most of recent empirical findings concerning financial time series: no correlation between price variations, long-range volatility correlations and multifractal statistics. Moreover, its extension to a multivariate context, in order to model portfolio behavior, is very natural. Comparisons to real data and other models proposed elsewhere are provided.
Changes in Dietary Fat Intake Alter Plasma Levels of Oxidized Low-Density Lipoprotein and Lipoprotein(a)

Objective—To assess the effects of dietary modifications on oxidized low-density lipoprotein (LDL). Methods and Results—Thirty-seven healthy women were fed two diets. Both diets contained a reduced amount of total and saturated fat. In addition, one diet was low in vegetables and the other was high in vegetables, berries, and fruit. The dietary intake of total fat was 70 g per day at baseline and decreased to 56 g (low-fat, low-vegetable diet) and to 59 g (low-fat, high-vegetable diet). The saturated fat intake decreased from 28 g to 20 g and to 19 g, and the amount of polyunsaturated fat intake increased from 11 g to 13 g and to 19 g (baseline; low-fat, low-vegetable; low-fat, high-vegetable; respectively). The amount of oxidized LDL in plasma was determined as the content of oxidized phospholipid per ApoB-100 using a monoclonal antibody EO6 (OxLDL-EO6). The median plasma OxLDL-EO6 increased by 27% (P <0.01) in response to the low-fat, low-vegetable diet and 19% (P <0.01) in response to the low-fat, high-vegetable diet. Also, the Lp(a) concentration was increased by 7% (P <0.01) and 9% (P= 0.01), respectively. Conclusion—Alterations in the dietary fat intake resulted in increased plasma concentrations of lipoprotein(a) and OxLDL-EO6.
Distortion-rate models for entropy-coded lattice vector quantization

The increasing demand for real-time applications requires the use of variable-rate quantizers having good performance in the low bit rate domain. In order to minimize the complexity of quantization, as well as maintaining a reasonably high PSNR ratio, we propose to use an entropy-coded lattice vector quantizer (ECLVQ). These quantizers have proven to outperform the well-known EZW algorithm's performance in terms of rate-distortion tradeoff. In this paper, we focus our attention on the modeling of the mean squared error (MSE) distortion and the prefix code rate for ECLVQ. First, we generalize the distortion model of Jeong and Gibson (1993) on fixed-rate cubic quantizers to lattices under a high rate assumption. Second, we derive new rate models for ECLVQ, efficient at low bit rates without any high rate assumptions. Simulation results prove the precision of our models.
Neutralization of TNF does not influence endotoxininduced changes in thyroid hormone metabolism in humans.

To determine the role of tumor necrosis factor (TNF) in endotoxin-induced changes in plasma thyroid hormone and thyroid-stimulating hormone (TSH) concentrations, 24 healthy postabsorptive humans were studied on a control study day ( n= 6), after infusion of a recombinant TNF receptor IgG fusion protein (TNFR:Fc; 6 mg/m2; n = 6) after intravenous injection of endotoxin (2 ng/kg; n = 6), or after administration of endotoxin with TNFR:Fc ( n = 6). Administration of TNFR:Fc alone did not affect thyroid hormone or TSH levels when compared with the control day. Endotoxin induced a transient rise in plasma TNF activity (1.5 h: 219 ± 42 pg/ml), which was completely prevented by TNFR:Fc ( P < 0.05). After endotoxin administration, plasmal-thyroxine (T4), free T4, 3,5,3'-triiodothyronine (T3), and TSH were lower and 3,3',5'-triiodothyronine was higher than on the control day (all P < 0.05). Coinfusion of TNFR:Fc with endotoxin did not influence these endotoxin-induced changes. Our results suggest that endogenous TNF does not play an important role in the alterations in plasma thyroid hormone and TSH concentrations induced by mild endotoxemia in healthy humans.
Human microvascular endothelial tissue culture cell model for studying pathogenesis of Brazilian purpuric fever

Brazilian purpuric fever (BPF) is a fulminant pediatric disease characterized by fever, with rapid progression to purpura, hypotensive shock, and death. All known BPF cases have been caused by three clones of Haemophilus influenzae biogroup aegyptius and have occurred in either Brazil or Australia. Using an immortalized line of human vascular endothelial cells, we developed an in vitro assay that identifies all known BPF-causing H. influenzae biogroup aegyptius strains (R. S. Weyant, F. D. Quinn, E. A. Utt, M. Worley, V. G. George, F. J. Candal, and E. W. Ades, J. Infect. Dis. 169:430-433, 1994). With multiplicities of infection (MOIs) as low as one bacterium per 1,000 tissue culture cells, BPF-associated strains produce a unique cytotoxic effect in which the tissue culture cells detach and aggregate in large floating masses after 48 h of incubation. In this study, using a BPF-associated strain and a non-BPF-associated control, we demonstrated that strains which produce the cytotoxic phenotype were able to replicate intracellularly whereas non-BPF-associated strains, with MOIs of > or = 1,000 did not replicate and did not produce the phenotype. We also showed that this phenotype is not caused by the activity of an endotoxin or the release of some other compound from the bacterial cell, since neither gamma irradiation-killed whole BPF clone bacteria nor bacterial cell fractions at MOIs of > 1,000 produced the cytotoxic effect. Furthermore, bacteria in numbers equal to MOIs of > 1,000 treated with chloramphenicol did not produce the cytotoxic phenotype, suggesting a requirement for bacterial protein synthesis. In addition, viable bacteria separated from the tissue culture monolayer by a 0.2-micron-pore-size membrane also failed to produce the phenotype. The ability of the bacterium to invade, replicate, and produce the phenotype appears to be primarily parasite directed since phagocytosis, pinocytosis, and eukaryotic protein synthesis inhibitors, including cycloheximide, cytochalasin D, and methylamine, had no effect on the ability of the bacterium to invade and cause a cytotoxic response. Understanding the basic mechanisms involved in this tissue-destructive process should enhance our knowledge of the general pathogenesis of BPF.
Discrimination factors of carbon and nitrogen stable isotopes in meerkat feces

Stable isotope analysis of feces can provide a non-invasive method for tracking the dietary habits of nearly any mammalian species. While fecal samples are often collected for macroscopic and genetic study, stable isotope analysis can also be applied to expand the knowledge of species-specific dietary ecology. It is somewhat unclear how digestion changes the isotope ratios of animals’ diets, so more controlled diet studies are needed. To date, most diet-to-feces controlled stable isotope experiments have been performed on herbivores, so in this study I analyzed the carbon and nitrogen stable isotope ratios in the diet and feces of the meerkat (Suricata suricatta), a small omnivorous mammal. The carbon trophic discrimination factor between diet and feces (Δ13Cfeces) is calculated to be 0.1 ± 1.5‰, which is not significantly different from zero, and in turn, not different than the dietary input. On the other hand, the nitrogen trophic discrimination factor (Δ15Nfeces) is 1.5 ± 1.1‰, which is significantly different from zero, meaning it is different than the average dietary input. Based on data generated in this experiment and a review of the published literature, carbon isotopes of feces characterize diet, while nitrogen isotope ratios of feces are consistently higher than dietary inputs, meaning a discrimination factor needs to be taken into account. The carbon and nitrogen stable isotope values of feces are an excellent snapshot of diet that can be used in concert with other analytical methods to better understand ecology, diets, and habitat use of mammals.