Miracle-dz/UniFAR-FTUs huggingface dataset:
Atf4 Regulates Obesity, Glucose Homeostasis, and Energy Expenditure

OBJECTIVE We evaluate a potential role of activating transcription factor 4 (Atf4) in invertebrate and mammalian metabolism. RESEARCH DESIGN AND METHODS With two parallel approaches—a fat body–specific green fluorescent protein enhancer trap screen in D. melanogaster and expression profiling of developing murine fat tissues—we identified Atf4 as expressed in invertebrate and vertebrate metabolic tissues. We assessed the functional relevance of the evolutionarily conserved expression by analyzing Atf4 mutant flies and Atf4 mutant mice for possible metabolic phenotypes. RESULTS Flies with insertions at the Atf4 locus have reduced fat content, increased starvation sensitivity, and lower levels of circulating carbohydrate. Atf4 null mice are also lean, and they resist age-related and diet-induced obesity. Atf4 null mice have increased energy expenditure potentially accounting for the lean phenotype. Atf4 null mice are hypoglycemic, even before substantial changes in fat content, indicating that Atf4 regulates mammalian carbohydrate metabolism. In addition, the Atf4 mutation blunts diet-induced diabetes as well as hyperlipidemia and hepatosteatosis. Several aspects of the Atf4 mutant phenotype resemble mice with mutations in components of the target of rapamycin (TOR) pathway. Consistent with the phenotypic similarities, Atf4 null mice have reduced expression of genes that regulate intracellular amino acid concentrations and lower intracellular concentration of amino acids, a key TOR input. Further, Atf4 mutants have reduced S6K activity in liver and adipose tissues. CONCLUSIONS Atf4 regulates age-related and diet-induced obesity as well as glucose homeostasis in mammals and has conserved metabolic functions in flies.
Physical and psychosocial factors associated with wrist or hand pain among Australian hospital-based nurses

Objective To assess the personal, physical and psychosocial factors associated with wrist or hand pain in Australian hospital-based nurses. Methods Wrist or hand pain, associated disability and sickness absence, demographic, occupational, physical, psychosocial and personal factors among nurses working for three hospitals in Melbourne, Australia, were assessed in a cross-sectional study. Factors associated with wrist or hand pain in the past month were assessed using logistic regression. Results This analysis was based on 1111 participants. The prevalence of wrist or hand pain in the past month was 15.3%. Repeated movements of the wrist or finger >4 h (OR 2.63, 95% CI 1.80 to 3.84), high job strain (1.54, 1.04 to 2.28), job insecurity (1.55, 1.04 to 2.28), somatisation tendency (2.73, 1.75 to 4.26), pain catastrophising (1.56, 1.03 to 2.37), better mental (0.97, 0.95 to 0.99) and physical (0.96, 0.94-0.98) health and well-being were associated with wrist or hand pain in the past month, after adjusting for possible confounding factors. When all significant factors were examined in the same model, repeated movements of the wrist or finger >4 h (2.50, 1.71 to 3.67), somatisation (2.61, 1.65 to 4.13) and better physical health and well-being (0.96, 0.94 to 0.99) remained independently associated with wrist or hand pain in the past month. Conclusions This study highlights that wrist or hand pain is prevalent in hospital nurses. Workplace physical factors and personal factors were associated with wrist or hand pain. Further longitudinal investigation is needed to examine the predictive nature of these factors.
NOX4 in Mitochondria: Yeast Two-Hybrid-Based Interaction with Complex I Without Relevance for Basal Reactive Oxygen Species?

Abstract NADPH oxidases (NOXs) represent the only known dedicated source of reactive oxygen species (ROS) and thus a prime therapeutic target. Type 4 NOX is unique as it produces H2O2, is constitutively active, and has been suggested to localize to cardiac mitochondria, thus possibly linking mitochondrial and NOX-derived ROS formation. The aim of this study was to identify NOX4-binding proteins and examine the possible physiological localization of NOX4 to mitochondria and its impact on mitochondrial ROS formation. We here provide evidence that NOX4 can, in principle, enter protein–protein interactions with mitochondrial complex I NADH dehydrogenase subunits, 1 and 4L. However, under physiological conditions, NOX4 protein was neither detectable in the kidney nor in cardiomyocyte mitochondria. The NOX inhibitor, GKT136901, slightly reduced ROS formation in cardiomyocyte mitochondria, but this effect was observed in both wild-type and Nox4−/− mice. NOX4 may thus associate with mitochondrial complex I proteins, but in cardiac and renal mitochondria under basal conditions, expression is beyond our detection limits and does not contribute to ROS formation. Antioxid. Redox Signal. 23, 1106–1112.
Achieving k-Anonymity Privacy Protection Using Generalization and Suppression

Often a data holder, such as a hospital or bank, needs to share person-specific records in such a way that the identities of the individuals who are the subjects of the data cannot be determined. One way to achieve this is to have the released records adhere to k- anonymity, which means each released record has at least (k-1) other records in the release whose values are indistinct over those fields that appear in external data. So, k- anonymity provides privacy protection by guaranteeing that each released record will relate to at least k individuals even if the records are directly linked to external information. This paper provides a formal presentation of combining generalization and suppression to achieve k-anonymity. Generalization involves replacing (or recoding) a value with a less specific but semantically consistent value. Suppression involves not releasing a value at all. The Preferred Minimal Generalization Algorithm (MinGen), which is a theoretical algorithm presented herein, combines these techniques to provide k-anonymity protection with minimal distortion. The real-world algorithms Datafly and µ-Argus are compared to MinGen. Both Datafly and µ-Argus use heuristics to make approximations, and so, they do not always yield optimal results. It is shown that Datafly can over distort data and µ-Argus can additionally fail to provide adequate protection.
Design of composite patch reinforcement applied to mixed-mode cracked panel using finite element analysis

Aircraft repair is gaining importance for extending the fatigue life of aging aircrafts and also for improving its structural integrity. Among various repair techniques, bonded composite repair is mostly preferred. In this paper, a three-dimensional finite element analysis is carried out to study and compare the performance of single- and double-sided patch on center-cracked aluminum panel. The effects of different parameters such as patch layup, patch thickness and patch material on the stress intensity factor is highlighted. It is shown that the mechanics of double-sided repair is completely different from the single-sided repair. Also, it is found that double-sided repair is more efficient than single-sided repair. In case of single-sided repair, alternative patch material like unbalanced lamina or transversely graded material is proposed. It is observed that there is significant reduction of stress intensity factor with transversely graded patch in case of single-sided repair.
The Stem Species of Our Species: A Place for the Archaic Human Cranium from Ceprano, Italy

One of the present challenges in the study of human evolution is to recognize the hominin taxon that was ancestral to Homo sapiens. Some researchers regard H. heidelbergensis as the stem species involved in the evolutionary divergence leading to the emergence of H. sapiens in Africa, and to the evolution of the Neandertals in Europe. Nevertheless, the diagnosis and hypodigm of H. heidelbergensis still remain to be clarified. Here we evaluate the morphology of the incomplete cranium (calvarium) known as Ceprano whose age has been recently revised to the mid of the Middle Pleistocene, so as to test whether this specimen may be included in H. heidelbergensis. The analyses were performed according to a phenetic routine including geometric morphometrics and the evaluation of diagnostic discrete traits. The results strongly support the uniqueness of H. heidelbergensis on a wide geographical horizon, including both Eurasia and Africa. In this framework, the Ceprano calvarium – with its peculiar combination of archaic and derived traits – may represent, better than other penecontemporaneous specimens, an appropriate ancestral stock of this species, preceding the appearance of regional autapomorphic features.
Fixation Probability for Lytic Viruses: The Attachment-Lysis Model

The fixation probability of a beneficial mutation is extremely sensitive to assumptions regarding the organism's life history. In this article we compute the fixation probability using a life-history model for lytic viruses, a key model organism in experimental studies of adaptation. The model assumes that attachment times are exponentially distributed, but that the lysis time, the time between attachment and host cell lysis, is constant. We assume that the growth of the wild-type viral population is controlled by periodic sampling (population bottlenecks) and also include the possibility that clearance may occur at a constant rate, for example, through washout in a chemostat. We then compute the fixation probability for mutations that increase the attachment rate, decrease the lysis time, increase the burst size, or reduce the probability of clearance. The fixation probability of these four types of beneficial mutations can be vastly different and depends critically on the time between population bottlenecks. We also explore mutations that affect lysis time, assuming that the burst size is constrained by the lysis time, for experimental protocols that sample either free phage or free phage and artificially lysed infected cells. In all cases we predict that the fixation probability of beneficial alleles is remarkably sensitive to the time between population bottlenecks.
Superoxide Scavenging and Antiglycation Activity of Rhinacanthins-rich Extract Obtained from the Leaves of Rhinacanthus nasutus

Background: Oxidative stress and nonenzymatic protein glycation lead to serious diabetic complications that increase the risk of mortality. Rhinacanthus nasutus leaf crude extracts are previously reported for their antidiabetic, antiglycation, and antioxidant potential. Objective: The present study was performed to prepare a standardized rhinacanthins-rich extract (RRE) and evaluate its superoxide scavenging and antiglycation effects as compared to its marker compounds, namely, rhinacanthin-C (RC), rhinacanthin-D (RD), and rhinacanthin-N (RN). Materials and Methods: RRE was obtained by microwave-assisted green extraction along with a simple step of fractionation using Amberlite® column. RC, RD, and RN were isolated from the RRE using silica gel column chromatography. Superoxide scavenging activity was performed by cyclic voltammetry, and fructose-mediated human serum albumin glycation model was used for antiglycation activity. In silico studies were conducted to identify the structure-activity relationships of rhinacanthins. Results: On the basis of kinetic measurements, RRE exhibited the most potent antioxidant activity via ErCi mechanism, with a 50% inhibitory concentration (IC50) value of 8.0 μg/mL, antioxidant capacity of 39439 M−1, and binding constant of 45709 M−1. Antiglycation assay showed that RRE exhibited almost equivalent glycation inhibitory effect to that of RC, with IC50values of 39.7 and 37.3 μg/mL, respectively, but higher than that of RD (IC50 of 50.4 μg/mL), RN (IC50 of 89.5 μg/mL), as well as the positive control, rutin (IC50of 41.5 μg/mL). Conclusions: The potent superoxide scavenging and albumin glycation inhibitory effect of RRE rationalized its therapeutic application in various chronic diseases, especially in the complications of diabetes. Abbreviations used: RRE: Rhinacanthins-rich extract; RC: Rhinacanthin-C; RD: Rhinacanthin-D; RN: Rhinacanthin-N; IC50: 50% inhibitory concentration; Kao: Antioxidant activity coefficient; Kb: Binding constant; ErCi: Reversible electron transfer followed by an irreversible chemical reaction; DM: Diabetes mellitus; AGEPs: Advanced glycation end products; NMR: Nuclear magnetic resonance; HPLC: High-performance liquid chromatography; CV: Cyclic voltammetry; DMSO: Dimethyl sulfoxide; Ipa: Anodic peak current; Ipc: Cathodic peak current; HSA: Human serum albumin; MOE: Molecular operating environment; PASSonline: Online prediction of activity spectra for substances.
The Impact of E-Commerce Announcements on the Market Value of Firms

The media hype surrounding the growth of electronic commerce has led to considerable firm interest in making the significant investments required to participate in this growing market. However, the evidence on benefits to firms from e-commerce is far from unequivocally positive, as popular accounts would lead us to believe. In this paper, we explore the following questions: What are the economic returns to firms from engaging in ecommerce? How do the returns to non-net, brick and mortar firms from e-commerce initiatives compare with returns to the new breed of net firms? How do returns from business-to-business e-commerce compare with returns from business-to-consumer e-commerce? We examine these issues using event study methodology and assess the cumulative abnormal returns (CARs) for 305 e-commerce announcements between October and December 1998. The results suggest that e-commerce initiatives announced in this period do indeed lead to positive CARs for firms. However, the hypothesis drawing on the resource-based view of the firm: that the CAR to non-net firms is significantly more than the CAR to net firms is not supported. Further, the CARs associated with business-to-consumer e-commerce announcements are higher than the CARs for business-to-business ecommerce, a result contrary to the hypothesized direction. The results are robust to the removal of outliers and time windows of varying length between firm announcements and capital market adjustments of prices. Most importantly, the magnitudes of CARs (between 3% and 11%) observed in response to e-commerce announcements are considerably larger than those observed for a variety of firm actions in the prior literature. This paper presents the first empirical test of the dot com effect, validating the popular notion that capital markets recognize the transformational potential of e-commerce and expect significant future benefits to firms entering into ecommerce arrangements. What did the CEO say to the CIO? Nice website, but what is it doing for our business?
New placement and global routing algorithms for standard cell layouts

The placement algorithm, called the hierarchical clustering with min-cut exchange (HCME), is effective at avoiding being trapped in local optimum solutions. The global routing algorithm does not route the nets one by one and therefore the results are independent of the net order and channel order. In this algorithm, channel width is minimized under a cost function, in which the trade-off between the minimization of net-lengths and the minimization of the number of tracks is considered. These algorithms are simple and highly efficient. This is confirmed by computational experiments. <<ETX>>
The reactivation of somatosensory cortex and behavioral recovery after sensory loss in mature primates

In our experiments, we removed a major source of activation of somatosensory cortex in mature monkeys by unilaterally sectioning the sensory afferents in the dorsal columns of the spinal cord at a high cervical level. At this level, the ascending branches of tactile afferents from the hand are cut, while other branches of these afferents remain intact to terminate on neurons in the dorsal horn of the spinal cord. Immediately after such a lesion, the monkeys seem relatively unimpaired in locomotion and often use the forelimb, but further inspection reveals that they prefer to use the unaffected hand in reaching for food. In addition, systematic testing indicates that they make more errors in retrieving pieces of food, and start using visual inspection of the rotated hand to confirm the success of the grasping of the food. Such difficulties are not surprising as a complete dorsal column lesion totally deactivates the contralateral hand representation in primary somatosensory cortex (area 3b). However, hand use rapidly improves over the first post-lesion weeks, and much of the hand representational territory in contralateral area 3b is reactivated by inputs from the hand in roughly a normal somatotopic pattern. Quantitative measures of single neuron response properties reveal that reactivated neurons respond to tactile stimulation on the hand with high firing rates and only slightly longer latencies. We conclude that preserved dorsal column afferents after nearly complete lesions contribute to the reactivation of cortex and the recovery of the behavior, but second-order sensory pathways in the spinal cord may also play an important role. Our microelectrode recordings indicate that these preserved first-order, and second-order pathways are initially weak and largely ineffective in activating cortex, but they are potentiated during the recovery process. Therapies that would promote this potentiation could usefully enhance recovery after spinal cord injury.
Draft Genome Sequence of Vibrio parahaemolyticus Strain M1-1, Which Causes Acute Hepatopancreatic Necrosis Disease in Shrimp in Vietnam

ABSTRACT We report here the genome sequence of Vibrio parahaemolyticus strain M1-1, which causes a mild form of shrimp acute hepatopancreatic necrosis disease (AHPND). Compared to other virulent strains, the M1-1 genome appeared to express several additional genes, while some genes were missing. These instabilities may be related to the reduced virulence of M1-1.
Solder joint reliability in AgPt‐metallized LTCC modules

Purpose – To investigate the effect of the metallization and solder mask materials on the solder joint reliability of low temperature co‐fired ceramic (LTCC) modules.Design/methodology/approach – The fatigue performance of six LTCC/PCB assembly versions was investigated using temperature cycling tests in the −40‐125°C and 20‐80°C temperature ranges. In order to eliminate fatigue cracking in the LTCC module itself, large AgPt‐metallized solder (1 mm) lands with organic or co‐fired glaze solder masks, having 0.86‐0.89 mm openings, were used. The performance of these modules was compared to that of AgPd‐metallized modules with a similar solder land structure. The joint structures were analysed using resistance measurements, scanning acoustic microscopy, SEM/EDS investigation, and FEM simulations.Findings – The results showed that failure distributions with Weibull shape factor (β) values from 8.4 to 14.2, and characteristic life time (θ) values between 860 and 1,165 cycles were achieved in AgPt assemblies in.
SVO2-Guided Resuscitation for Experimental Septic Shock: Effects of Fluid Infusion and Dobutamine on Hemodynamics, Inflammatory Response, and Cardiovascular Oxidative Stress

ABSTRACT The pathogenetic mechanisms associated to the beneficial effects of mixed venous oxygen saturation (SvO2)–guided resuscitation during sepsis are unclear. Our purpose was to evaluate the effects of an algorithm of SvO2-driven resuscitation including fluids, norepinephrine and dobutamine on hemodynamics, inflammatory response, and cardiovascular oxidative stress during a clinically resembling experimental model of septic shock. Eighteen anesthetized and catheterized pigs (35–45 kg) were submitted to peritonitis by fecal inoculation (0.75 g/kg). After hypotension, antibiotics were administered, and the animals were randomized to two groups: control (n = 9), with hemodynamic support aiming central venous pressure 8 to 12 mmHg, urinary output 0.5 mL/kg per hour, and mean arterial pressure greater than 65 mmHg; and SvO2 (n = 9), with the goals above, plus SvO2 greater than 65%. The interventions lasted 12 h, and lactated Ringer’s and norepinephrine (both groups) and dobutamine (SvO2 group) were administered. Inflammatory response was evaluated by plasma concentration of cytokines, neutrophil CD14 expression, oxidant generation, and apoptosis. Oxidative stress was evaluated by plasma and myocardial nitrate concentrations, myocardial and vascular NADP(H) oxidase activity, myocardial glutathione content, and nitrotyrosine expression. Mixed venous oxygen saturation–driven resuscitation was associated with improved systolic index, oxygen delivery, and diuresis. Sepsis induced in both groups a significant increase on IL-6 concentrations and plasma nitrate concentrations and a persistent decrease in neutrophil CD14 expression. Apoptosis rate and neutrophil oxidant generation were not different between groups. Treatment strategies did not significantly modify oxidative stress parameters. Thus, an approach aiming SvO2 during sepsis improves hemodynamics, without any significant effect on inflammatory response and oxidative stress. The beneficial effects associated with this strategy may be related to other mechanisms.
Limitations of the linear and the projection approximations in three‐dimensional transmission electron microscopy of fully hydrated proteins

We establish expressions for the linear and quadratic terms in the series expansion of the phase and the phase and amplitude object description of imaging thin specimens by transmission electron microscopy. Based on these expressions we simulate the corresponding contributions to images of unstained protein complexes of varying thickness and arrive at an estimate for how much each term contributes to the contrast of the image. From this we can estimate a maximum specimen thickness for which the weak phase and the weak amplitude and phase object approximation (and therefore linear imaging) is still reasonably accurate.
Isolation and characterization of the Vibrio cholerae recA gene

A 3.6-kilobase PstI fragment was isolated from a Vibrio cholerae chromosomal DNA library and shown to encode RecA-like activity in complementation studies with Escherichia coli recA mutants. Although DNA hybridization experiments failed to detect any homology between the E. coli and V. cholerae recA genes, hyperimmune antiserum produced against purified E. coli RecA protein recognized epitopes shared by the V. cholerae protein. The V. cholerae chromosomal fragments, when cloned and transferred to E. coli, provided the missing recA functions, including resistance to the alkylating agent methyl methanesulfonate, resistance to UV irradiation, and promotion of homologous recombination in Hfr mating experiments.
Effect of light intensity on self-feeding of rainbow trout Oncorhynchus mykiss reared individually

The self-feeding abilities of individually reared rainbow trout Oncorhynchus mykiss were investigated under four different light intensities (7×10−2, 1×10°, 5×101, and 7×102 lx). There was no significant effect of light intensity on self-feeder learning ability and food wastage (which did not exceed 0.5%). However, there was high individual variability in the ability irrespective of light intensity. The total number of trigger actuations was significantly higher at 1×10° lx than at 7×10−2 and 5×101 lx. Feeding patterns during the light phase of a day were classified as early or late in relation to the timing of significant peaks in fish feeding, or non-steady. The last pattern was defined when significant peaks were not observed. Under 7×10−2, 1×10°, or 7×102 lx, a non-steady feeding pattern was the most common. Early feeding appeared only under the 1×10° lx regime with the random pattern, while the late pattern was observed under 7×10−2, 5×101, and 7×102 lx. Under 5×101 lx regime half of the fish fed in the afternoon. These results suggest that light intensity can affect the timing of feeding judged from the frequency of pulling bite tags, but does not affect waste or learning period.