Miracle-dz/UniFAR-FTUs huggingface dataset:
Non-Stimulated, Agonist-Stimulated and Store-Operated Ca2+ Influx in MDA-MB-468 Breast Cancer Cells and the Effect of EGF-Induced EMT on Calcium Entry

In addition to their well-defined roles in replenishing depleted endoplasmic reticulum (ER) Ca2+ reserves, molecular components of the store-operated Ca2+ entry pathway regulate breast cancer metastasis. A process implicated in cancer metastasis that describes the conversion to a more invasive phenotype is epithelial-mesenchymal transition (EMT). In this study we show that EGF-induced EMT in MDA-MB-468 breast cancer cells is associated with a reduction in agonist-stimulated and store-operated Ca2+ influx, and that MDA-MB-468 cells prior to EMT induction have a high level of non-stimulated Ca2+ influx. The potential roles for specific Ca2+ channels in these pathways were assessed by siRNA-mediated silencing of ORAI1 and transient receptor potential canonical type 1 (TRPC1) channels in MDA-MB-468 breast cancer cells. Non-stimulated, agonist-stimulated and store-operated Ca2+ influx were significantly inhibited with ORAI1 silencing. TRPC1 knockdown attenuated non-stimulated Ca2+ influx in a manner dependent on Ca2+ influx via ORAI1. TRPC1 silencing was also associated with reduced ERK1/2 phosphorylation and changes in the rate of Ca2+ release from the ER associated with the inhibition of the sarco/endoplasmic reticulum Ca2+-ATPase (time to peak [Ca2+]CYT = 188.7±34.6 s (TRPC1 siRNA) versus 124.0±9.5 s (non-targeting siRNA); P<0.05). These studies indicate that EMT in MDA-MB-468 breast cancer cells is associated with a pronounced remodeling of Ca2+ influx, which may be due to altered ORAI1 and/or TRPC1 channel function. Our findings also suggest that TRPC1 channels in MDA-MB-468 cells contribute to ORAI1-mediated Ca2+ influx in non-stimulated cells.
The finite-length multi-input multi-output MMSE-DFE

A new theoretical framework is introduced for analyzing the performance of a finite length minimum-mean-square error decision feedback equalizer (MMSE-DFE) in a multi-input multi-output (MIMO) environment. The framework includes transmit and receive diversity systems as special cases and quantifies the diversity performance improvement as a function of the number of transmit/receive antennas and equalizer taps. Fast and parallelizable algorithms for computing the finite-length MIMO MMSE-DFE are presented for three common multi-user detection scenarios.
Learning in Helicoverpa armigera (Lepidoptera: Noctuidae): a new look at the behaviour and control of a polyphagous pest

Recent experimental evidence has shown that learning occurs in the host selection behaviour of Helicoverpa armigera (Hubner), one of the world‘s most important agricultural pests. This paper discusses how the occurrence of learning changes our understanding of the host selection behaviour of this polyphagous moth. Host preferences determined from previous laboratory studies may be vastly different from preferences exhibited by moths in the field, where the abundance of particular hosts may be more likely to determine host preference. In support of this prediction, a number of field studies have shown that the ‘attractiveness’ of different hosts for H. armigera oviposition may depend on the relative abundance of these host species. Insect learning may play a fundamental role in the design and application of present and future integrated pest management strategies such as the use of host volatiles, trap crops and resistant crop varieties for monitoring and controlling this important pest species.
Reducing Satiation: The Role of Categorization Level

People usually like experiences less as they repeat them: they satiate. This research finds that people satiate less if they categorize the consumption episodes at lower levels. For instance, as people ate more jelly beans, their enjoyment declined less quickly when the candy was categorized specifically (e.g., cherry, orange) rather than generally (e.g., jelly bean). Three studies demonstrate this "specificity effect" for people's ratings of enjoyment both during and immediately after consumption. Process evidence shows that subcategorization focuses people's attention on differentiating aspects, making the episodes seem less repetitive and consequently less satiating. (c) 2008 by JOURNAL OF CONSUMER RESEARCH, Inc.
Correlation between Plantar Foot Temperature and Diabetic Neuropathy: A Case Study by Using an Infrared Thermal Imaging Technique

Background: Diabetic neuropathy consists of multiple clinical manifestations of which loss of sensation is most prominent. High temperatures under the foot coupled with reduced or complete loss of sensation can predispose the patient to foot ulceration. The aim of this study was to look at the correlation between plantar foot temperature and diabetic neuropathy using a noninvasive infrared thermal imaging technique. Methods: Infrared thermal imaging, a remote and noncontact experimental tool, was used to study the plantar foot temperatures of 112 subjects with type 2 diabetes selected from a tertiary diabetes centre in South India. Results: Patients with diabetic neuropathy (defined as vibration perception threshold (VPT) values on biothesiometry greater than 20 V) had a higher foot temperature (32–35 °C) compared to patients without neuropathy (27–30 °C). Diabetic subjects with neuropathy also had higher mean foot temperature (MFT) (p = .001) compared to non-neuropathic subjects. MFT also showed a positive correlation with right great toe (r = 0.301, p = .001) and left great toe VPT values (r = 0.292, p = .002). However, there was no correlation between glycated hemoglobin and MFT. Conclusion: Infrared thermal imaging may be used as an additional tool for evaluation of high risk diabetic feet.
Challenges for inclusive education

This study attempted to discover teachers' perceptions of the success of inclusive education inclusion of learning disabled students in the junior high schools of Westwind School Division #74. Several areas were explored. They included the extent to which inclusion is implemented across the Division, the teachers' perceptions of the academic and social success of the learning disabled students, the teachers' perceptions of the effects on the regular students, the teachers' evaluation of the extent to which factors stated in literature as being essential to the success of inclusion were present in their schools, and the changes needed for more successful inclusion. The sample for this study comprised all of the junior high teachers in the division who taught one or more of the core subject areas (science, social studies, mathematics, language arts) in Grades 7-9. All completed a written survey, then a sub-group were interviewed. The results demonstrated that although 82.93% of the teachers believed the regular classroom was the rightful place for the learning disabled students to learn, 80.48% felt that they were unable to meet the needs of these students. To improve the quality of the inclusive programming the respondents felt they required more planning and collaboration time, an increase in knowledge regarding programming adjustments for learning disabled students, reduced class sizes and other professional development activities to meet their individual needs.
The Effect of Simulating Different Intermediate Host Snail Species on the Link between Water Temperature and Schistosomiasis Risk

Introduction A number of studies have attempted to predict the effects of climate change on schistosomiasis risk. The importance of considering different species of intermediate host snails separately has never previously been explored. Methods An agent-based model of water temperature and Biomphalaria pfeifferi population dynamics and Schistosoma mansoni transmission was parameterised to two additional species of snail: B. glabrata and B. alexandrina. Results Simulated B. alexandrina populations had lower minimum and maximum temperatures for survival than B. pfeifferi populations (12.5–29.5°C vs. 14.0–31.5°C). B. glabrata populations survived over a smaller range of temperatures than either B. pfeifferi or B. alexandrina (17.0°C–29.5°C). Infection risk peaked at 16.5°C, 25.0°C and 19.0°C respectively when B. pfeifferi, B. glabrata and B. alexandrina were simulated. For all species, infection risk increased sharply once a minimum temperature was reached. Conclusions The results from all three species suggest that infection risk may increase dramatically with small increases in temperature in areas at or near the currents limits of schistosome transmission. The effect of small increases in temperature in areas where schistosomiasis is currently found will depend both on current temperatures and on the species of snail acting as intermediate host(s) in the area. In most areas where B. pfeifferi is the host, infection risk is likely to decrease. In cooler areas where B. glabrata is the host, infection risk may increase slightly. In cooler areas where B. alexandrina is the host, infection risk may more than double with only 2°C increase in temperature. Our results show that it is crucial to consider the species of intermediate host when attempting to predict the effects of climate change on schistosomiasis.
Integrating TAM, TPB and TOE frameworks and expanding their characteristic constructs for e-commerce adoption by SMEs

Purpose – This paper aims to propose a framework that integrated the technology acceptance model (TAM), theory of planned behaviour (TPB) and technology-organization-environment (TOE) and extended the constructs to enrich literature and capture some peculiarities of small and medium-scale enterprises (SMEs). Individually, the frameworks of extant TAM, TPB and TOE are insightful to the understanding of e-commerce adoption but a bit parochial in their constructs and so, can rarely provide clear lenses to deal with SMEs. Design/methodology/approach – The adoption of e-commerce depends largely on the users’ conscious assessment of the influencing constructs as proposed, among others, in theories of reasoned action, TAM, TPB and TOE. This paper reviewed, synthesized and extended the constructs of these models in an integrated framework. The proposed integrated framework led to 18 propositions to promote and facilitate future research, and to guide explanation and prediction of e-commerce adoption in an organiz.
Exploring the differential associations between components of executive functioning and reactive and proactive aggression

ABSTRACT Objective: A large body of literature confirms the importance of executive functioning (EF) in the explanation of aggressive and antisocial behaviors. However, the common and specific associations between subtypes of aggression, such as reactive (RA), proactive aggression (PA), and EF are unclear. The current study explored the nuanced associations between components of EF and subtypes of aggression, using a latent variable approach. Method: Participants were 384 racially diverse undergraduate students (ages 18–52 years) who completed a self-report measure of RA and PA, and traditional neuropsychological tasks of EF. The appropriateness of using a nested bifactor model of EF was confirmed, and this bifactor model of EF was then used to examine the specific associations between components of EF and RA and PA. Results: Results revealed that components of EF are differentially associated with RA and PA. Specifically, impulsive, provoked aggression (i.e., RA) was associated with lower levels of goal-oriented inhibition and higher levels of flexibility, whereas planned, goal-oriented aggression (i.e., PA) was associated with higher levels of working memory. Conclusions: Findings from the current study underscore the importance of considering the multidimensional nature of EF, as well as the heterogeneity within aggression, rather than considering either construct as a single monolithic construct. The current study suggests that potentially unique brain-based pathways from aspects of EF to subtypes of aggression may exist, and points toward potential avenues through which to intervene.
Understanding the Link Between Initial ERP Systems and ERP-Enabled Adoption

ERP systems have been identified as platform technologies that permit the adoption of subsequent technologies that leverage the information integration created by an ERP system. Although significant research attention has been directed at ERP system adoption, very little attention has been paid to understanding ERP-enabled adoption, that is, adoptions that occur and are facilitated after the initial ERP system. This paper seeks to fill this void. Synthesizing existing works, the authors construct a model that examines the link between initial ERP system and ERP-enabled adoption. The results indicate that initial ERP system factors Extent of ERP Implementation and Current System Performance act as antecedences to organizational ease of use and relative advantage of subsequent technologies. Moreover, the authors' findings implicate that organizational ease of use and relative advantage of subsequent technologies have a positive impact on ERP-enabled adoption. These results have important implications for ERP system evaluation and justification. First, the findings highlight the importance of recognizing the potential additions and inherent benefits created by an initial ERP system. Second, the findings also underscore the importance of considering ERP-enabled adoption of subsequent technologies.
Delayed Effects of Anoxia Upon the Electrical Properties of an Identified Cockroach Motoneurone

SUMMARY 1. In nerve preparations isolated from cockroaches that had survived exposure to carbon dioxide or nitrogen for 1—3 h, the cell body of the metathoracic 'fast' coxal depressor motoneurone (Df) gained the ability to support all-or-none action potentials. Such responses were observed between 10 h and 5 days after the anoxic period. 2. Recordings from isolated cell bodies indicated that the action potentials were generated in the cell body itself rather than in adjacent regions of the neurone. 3. Action potentials were abolished in a sodium-free medium or in the presence of tetrodotoxin, but persisted in saline containing manganese ions, indicating that sodium ions are the major ions involved in the rising phase of these action potentials. 4. Elevation of the intracellular H + concentration by acid injection or by bathing the preparation in saline containing 40mmoll~ l ammonium chloride caused a marked depolarization of the membrane potential and action potentials could not be elicited. This indicates that a fall in intracellular pH during anoxia could not itself directly produce the enhancement in excitability reported here. 5. Histological examination of the neurone, after periods of anoxia sufficient to enable the cell body to generate the action potentials reported here, showed no detectable change in the pcrinuclear distribution of RNA and hence did not indicate any alteration in protein synthesis.
Characterization of an antibody that recognizes peptides containing D-β-aspartyl residues

Purpose Biologically uncommon D-β-aspartyl (D-β-Asp) residues have been detected in proteins from various human tissues from elderly donors and are connected with cataract, age-related macular degeneration, Alzheimer disease and UV-irradiated skin. In a previous study, we prepared a highly specific antibody against the peptide Gly-Leu-D-β-Asp-Ala-Thr-Gly-Leu-D-β-Asp-Ala-Thr-Gly-Leu-D-β-Asp-Ala-Thr (designated peptide 3R) that corresponds to three repeats of positions 149–153 of human lens αA-crystallin. This antibody clearly distinguishes between the different configurations of the Asp residue in that it reacted strongly with the D-β-Asp-containing peptides but did not react with L-α-Asp-, L-β-Asp-, or D-α-Asp-containing peptides. However, it remains unclear whether the antibody recognizes the amino acid sequences surrounding the D-β-Asp residue. The purpose of the present study is to elucidate the sequence dependency of the epitope of the antigen. Methods To clarify the properties of the anti-peptide 3R antibody, we used F-moc (9-fluorenylmethoxycarbonyl) solid phase chemistry to synthesize various peptides and analogs based on the peptides T18 (I146QTGLDATHAER157) and T6 (T55VLDAGISEVR65) which correspond to amino acid sequences 146–157 and 55–65, respectively of human αA-crystallin. The specificity of antibody was confirmed by ELISA (enzyme-linked immunosorbent assay) using these peptides. Results The anti peptide 3R antibody specifically recognized D-β-Asp residues and does not react with other configurations of Asp such as the L-α, L-β, D-α isomers in peptides. When the Ala in the peptide was replaced by other amino acid residues, the antibody did not react with the antigen. The antibody requires the sequence Leu-D-β-Asp-Ala to detect D-β-Asp containing proteins in living tissue. Conclusions The anti peptide 3R antibody is a powerful and easy tool for detection of D-β-Asp containing proteins in living tissues from patients with age-related diseases. However, to detect the D-β-Asp containing proteins in the living tissues using the anti-peptide 3R antibody, the protein must contain the sequence Leu-D-β-Asp-Ala.
Team working in intensive care: current evidence and future endeavors

Purpose of reviewIt has recently been argued that the future of intensive care medicine will rely on high quality management and teamwork. Therefore, this review takes an organizational psychology perspective to examine the most recent research on the relationship between teamwork, care processes, and patient outcomes in intensive care. Recent findingsInterdisciplinary communication within a team is crucial for the development of negotiated shared treatment goals and short-team patient outcomes. Interventions for maximizing team communication have received substantial interest in recent literature. Intensive care coordination is not a linear process, and intensive care teams often fail to discuss how to implement goals, trigger and align activities, or reflect on their performance. Despite a move toward interdisciplinary team working, clinical decision-making is still problematic and continues to be perceived as a top-down and authoritative process. The topic of team leadership in intensive care is underexplored and requires further research. SummaryBased on findings from the most recent research evidence in medicine and management, four principles are identified for improving the effectiveness of team working in intensive care: engender professional efficacy, create stable teams and leaders, develop trust and participative safety, and enable frequent team reflexivity
Post-T Tauri Stars in the Nearest OB Association

We present results of a spectroscopic survey of X-ray– and proper-motion–selected samples of late-type stars in the Lower Centaurus–Crux (LCC) and Upper Centaurus–Lupus (UCL) subgroups of the nearest OB association: Scorpius-Centaurus. The primary goals of the survey are to determine the star formation history of the OB subgroups and to assess the frequency of accreting stars in a sample dominated by "post–T Tauri" pre–main-sequence (PMS) stars. We investigate two samples: (1) proper-motion candidates from the ACT Catalog and Tycho Reference Catalog (TRC) with X-ray counterparts in the ROSAT All-Sky Survey (RASS) Bright Source Catalog and (2) G- and K-type stars in the Hipparcos catalog found to be candidate members by de Zeeuw et al. We obtained optical spectra of 130 candidates with the Siding Spring 2.3 m dual-beam spectrograph. PMS stars were identified by (1) strong Li λ6707 absorption, (2) subgiant surface gravities, (3) proper motions consistent with Sco-Cen membership, and (4) H-R diagram positions consistent with being PMS. We find 93% of the RASS-ACT/TRC stars to be probable PMS members, compared with 73% of the Hipparcos candidates. We demonstrate that measuring the gravity-sensitive band ratio of Sr II λ4077 to Fe I λ4071 is a valuable means of discriminating PMS and zero-age main-sequence (ZAMS) stars. Using secular parallaxes and Hipparcos, Tycho-2, and Two Micron All Sky Survey photometry, we construct an H-R diagram. Depending on the choice of published evolutionary tracks, we find the mean ages of the PMS populations to range between 17 and 23 Myr for LCC and 15 and 22 Myr for UCL. Taking into account observational errors, it appears that 95% of the low-mass star formation in each subgroup must have occurred in less than 8 Myr (LCC) and 12 Myr (UCL). Using the Bertelli et al. tracks, we find main-sequence turnoff ages for Hipparcos B-type members to be 16 ± 1 Myr for LCC and 17 ± 1 Myr for UCL. Contrary to previous findings, it appears that LCC is coeval with, or slightly older than, UCL. The secular parallaxes of the Sco-Cen PMS stars yield distances of 85–215 pc, with 12 of the LCC members lying within 100 pc of the Sun. Only one out of 110 (0.9%; 1 σ) PMS solar-type stars in the sample with ages of 13 ± 1 (s.e.) ± 6 (1 σ) Myr and masses of 1.3 ± 0.2 (1 σ) M⊙ shows both enhanced Hα emission and a K-band excess indicative of accretion from a truncated circumstellar disk: the nearby (d ≃ 86 pc) classical T Tauri star PDS 66.
Possibilities and limitations of passive restoration of heavily disturbed sites

ABSTRACT Passive restoration, which relies exclusively on natural processes and technical reclamation, which intervenes strongly into the restoration process, represent the extremes of a restoration action continuum. Between the extremes, we consider various degrees of active restoration. We suggest a general scheme to determine which approach to use based on site conditions, landscape context and societal circumstances. We conclude that passive restoration should usually be preferred in smaller sites with low abiotic stress and moderate productivity that are recovering from moderate disturbances. A passive approach may also be preferred in those landscapes that are less altered by humans, where target species are common and both invasive aliens and strong competitive generalists are rare. In such landscapes, passive restoration may succeed even if initial disturbances are intense. Potential of passive restoration has not yet been fully utilised. Passive restoration is viable economically and can produce multiple social and ecological benefits.
An empirical study of cost drivers in the U.S. Airline Industry

SYNOPSIS AND INTRODUCTION: Recent research on cost driver analysis by Miller and Vollman (1985) and Cooper and Kaplan (1987) suggests that transactions deriving from the diversity of a firm's product line and the complexity of its production process, in addition to output volume, drive overhead costs. As a consequence, it is argued, conventional cost accounting systems based only on volume-related measures, such as units of output, direct labor hours, or machine hours, produce biased and materially misleading cost estimates for managerial decisions on price and product line (whether to continue or discontinue products, or to offer additional products). Systematic biases in cost estimates may also lead to distortions in flexible budgeting systems, variance analyses, and responsibility-accounting systems. Perhaps more important in the long run, omission of operationsbased cost drivers may distort the investigation of the likely effects on costs of changes in operating strategies. Many firms have moved ahead on the basis of this perceived need for more accurate cost estimates and have designed and implemented activitybased costing systems (Schiff 1991). From an academic perspective, however, there is a need for further formal empirical research in this field. Cooper and Kaplan's (1987) evidence is based on field-study discussions with managers in a variety of manufacturing settings and experimentation with cost allocation and product-costing systems based on transactions. Foster and Gupta (1990) provide some of the first empirical evidence on the correlation of manufacturing overhead with output volume and operations-based measures that reflect characteristics of the manufacturing process. Using data obtained from 37 plants of a single manufacturing firm, Foster and Gupta found that most of the volume-related measures of output