Miracle-dz/UniFAR-FTUs huggingface dataset:
Gender Differences in Reactions to the Sexualization of Athletes

Participants rated a series of photographs that depicted well-known male and female athletes in a sexualized fashion or not. In general, sexualized targets were perceived as more attractive but as less competent than non-sexualized targets, and for ratings of competence, sexualization effects were greater for female targets. Sexualization had no effects on women’s perceptions of the competence of male targets, whereas for men, sexualization affected their perceptions of the competence of both male and female targets, although the influence of sexualization was stronger for female targets than it was for male targets. These findings suggest that the gender of the target and of the perceiver needs to be considered when evaluating the influences of sexualization. The implications of these findings are discussed within the context of objectification theory, which has focused on the objectification of women by women.
Response of marine viral populations to a nutrient induced phytoplankton bloom at different pCO 2 levels

Abstract. During the PeECE III mesocosm experiment in 2005 we investigated how the virioplankton community responded to increased levels of nutrients (N and P) and CO2. We applied a combination of flow cytometry, Pulsed Field Gel Electrophoresis and degenerate PCR primers to categorize and quantify individual viral populations, and to investigate their temporal dynamics. Species specific and degenerate primers enabled us to identify two specific large dsDNA viruses, EhV and CeV, infecting the haptophytes Emiliania huxleyi and Crysochromulina ericina, respectively. Some of the viral populations detected and enumerated by flow cytometry did not respond to altered CO2-levels, but the abundance of EhV and an unidentified dsDNA virus decreased with increasing CO2 levels. Our results thus indicate that CO2 conditions, or the related change in pH, may affect the marine pelagic food web at the viral level. Our results also demonstrate that in order to unravel ecological problems as how CO2 and nutrient levels affect the relationship between marine algal viruses and their hosts, we need to continue the effort to develop molecular markers used to identify both hosts and viruses.
Progesterone Receptor-B Regulation of Insulin-Like Growth Factor–Stimulated Cell Migration in Breast Cancer Cells via Insulin Receptor Substrate-2

Progesterone action contributes to the signaling of many growth factor pathways relevant to breast cancer tumor biology, including the insulin-like growth factor (IGF) system. Previous work has shown that insulin receptor substrate-2 (IRS-2) but not IRS-1 levels were regulated by progestin in progesterone receptor-B (PR-B) isoform expressing MCF-7 cells (C4-12 PR-B). Furthermore, type 1 IGF receptor (IGF1R) signaling via IRS-2 correlated with the increased cell migration observed in a number of breast cancer cell lines. Consequently, in this study, we examined whether the elevation of IRS-2 protein induced by progestin was sufficient to promote IGF-I–stimulated cell motility. Treatment of C4-12 PR-B cells with progestin shifted the balance of phosphorylation from IRS-1 to IRS-2 in response to IGF-I. This shift in IRS-2 activation was associated with enhanced migration in C4-12 PR-B cells pretreated with progestin, but had no effect on cell proliferation or survival. Treatment of C4-12 PR-B cells with RU486, an antiprogestin, inhibited IGF-induced cell migration. Attenuation of IRS-2 expression using small interfering RNA resulted in decreased IGF-stimulated motility. In addition, IRS-2 knockdown resulted in an abrogation of PKB/Akt phosphorylation but not mitogen-activated protein kinase. Consequently, LY294002, a phosphoinositide-3-kinase inhibitor, abolished IGF-induced cell motility in progestin-treated C4-12 PR-B cells. These data show a role for the PR in functionally promoting growth factor signaling, showing that levels of IRS proteins can determine IGF-mediated biology, PR-B signaling regulates IRS-2 expression, and that IRS-2 can mediate IGF-induced cell migration via phosphoinositide-3-kinase in breast cancer cells. (Mol Cancer Res 2008;6(9):1491–8)
Palliative Care for Hospitalized Patients With Stroke

Background and Purpose— Substantial variability exists in the use of life-prolonging treatments for patients with stroke, especially near the end of life. This study explores patterns of palliative care utilization and death in hospitalized patients with stroke across the United States. Methods— Using the 2010 to 2012 nationwide inpatient sample databases, we included all patients discharged with stroke identified by International Classification of Diseases-Ninth Revision codes. Strokes were subclassified as ischemic, intracerebral, and subarachnoid hemorrhage. We compared demographics, comorbidities, procedures, and outcomes between patients with and without a palliative care encounter (PCE) as defined by the International Classification of Diseases-Ninth Revision code V66.7. Pearson &khgr;2 test was used for categorical variables. Multivariate logistic regression was used to account for hospital, regional, payer, and medical severity factors to predict PCE use and death. Results— Among 395 411 patients with stroke, PCE was used in 6.2% with an increasing trend over time (P<0.05). We found a wide range in PCE use with higher rates in patients with older age, hemorrhagic stroke types, women, and white race (all P<0.001). Smaller and for-profit hospitals saw lower rates. Overall, 9.2% of hospitalized patients with stroke died, and PCE was significantly associated with death. Length of stay in decedents was shorter for patients who received PCE. Conclusions— Palliative care use is increasing nationally for patients with stroke, especially in larger hospitals. Persistent disparities in PCE use and mortality exist in regards to age, sex, race, region, and hospital characteristics. Given the variations in PCE use, especially at the end of life, the use of mortality rates as a hospital quality measure is questioned.
Investigation of the Dimer Interface and Substrate Specificity of Prolyl Dipeptidase DPP8*

DPP8 belongs to the family of prolyl dipeptidases, which are capable of cleaving the peptide bond after a penultimate proline residue. Unlike DPP-IV, a drug target for type II diabetes, no information is available on the crystal structure of DPP8, the regulation of its enzymatic activity, or its substrate specificity. In this study, using analytical ultracentrifugation and native gel electrophoresis, we show that the DPP8 protein is predominantly dimeric when purified or in the cell extracts. Four conserved residues in the C-terminal loop of DPP8 (Phe822, Val833, Tyr844, and His859), corresponding to those located at the dimer interface of DPP-IV, were individually mutated to Ala. Surprisingly, unlike DPP-IV, these single-site mutations abolished the enzymatic activity of DPP8 without disrupting its quaternary structure, indicating that dimerization itself is not sufficient for the optimal enzymatic activity of DPP8. Moreover, these mutations not only decreased kcat, as did the corresponding DPP-IV mutations, but also dramatically increased Km. We further show that the Km effect is independent of the substrate assayed. Finally, we identified the distinctive and strict substrate selectivity of DPP8 for hydrophobic or basic residues at the P2 site, which is in sharp contrast to the much less discriminative substrate specificity of DPP-IV. Our study has identified the residues absolutely required for the optimal activity of DPP8 and its unique substrate specificity. This study extends the functional importance of the C-terminal loop to the whole family of prolyl dipeptidases.
Reliability and validity of the Finnish version of the prosthesis evaluation questionnaire

Abstract Background: Thus far there have been no specific patient-reported outcome instrument in Finnish for health-related quality of life (HRQoL) assessment after major lower extremity amputation and successful prosthesis fitting. Methods: The prosthesis evaluation questionnaire (PEQ) was translated and cross-culturally adapted into Finnish. Participants completed a questionnaire package including the Finnish version of the PEQ and the 15 D HRQoL instrument. Scales (n = 10) were tested for internal consistency, floor-ceiling effect, and reproducibility for which participants completed the PEQ twice within a 2-week interval. Validity was tested by estimating the correlation between the 15 D index and the scales. The authors included 122 participants who had completed the questionnaire on two separate occasions in the final analysis. Results: Mean scale scores of the 10 scales varied from 52 to 83. Cronbach’s alphas ranged from 0.67 to 0.96. The total score showed no floor-ceiling effect. Reproducibility of the scales was good (intraclass correlation coefficient, 0.78–0.87; coefficient of repeatability, 19–36). Significant correlations were observed between the 15 D index and the scales for ambulation, social burden, usefulness, and well-being. Conclusions: This study provided evidence of the reliability and validity of the Finnish version of the PEQ in assessing the HRQoL among major lower extremity amputated patients who have been fitted with prosthesis. Implications for rehabilitation Measurement of quality of life during rehabilitation can provide important information on patients’ well-being. The prosthesis evaluation questionnaire (PEQ) is a valid instrument for assessing health-related quality of life (HRQoL) after major lower extremity amputation. This study provided evidence of the reliability and validity of the Finnish version of the PEQ for assessing HRQoL among patient who have undergone major lower extremity amputation.
UvA-DARE (Digital Academic Repository) Neural systems for social cognition in Klinefelter syndrome (47,XXY): evidence from fMRI

Klinefelter syndrome (KS) is a chromosomal condition (47, XXY) that may help us to unravel gene – brain behavior pathways to psychopathology. The phenotype includes social cognitive impairments and increased risk for autism traits. We used functional MRI to study neural mechanisms underlying social information processing. Eighteen nonclinical controls and thirteen men with XXY were scanned during judgments of faces with regard to trustworthiness and age. While judging faces as untrustworthy in comparison to trustworthy, men with XXY displayed less activation than controls in (i) the amygdala, which plays a key role in screening information for socio-emotional significance, (ii) the insula, which plays a role in subjective emotional experience, as well as (iii) the fusiform gyrus and (iv) the superior temporal sulcus, which are both involved in the perceptual processing of faces and which were also less involved during age judgments in men with XXY. This is the first study showing that KS can be associated with reduced involvement of the neural network subserving social cognition. Studying KS may increase our understanding of the genetic and hormonal basis of neural dysfunctions contributing to abnormalities in social cognition and behavior, which are considered core abnormalities in psychiatric disorders such as autism and schizophrenia.
Challenge of culture, conscience, and contract to general practitioners' care of Challenge of culture, conscience, and contract to general practitioners' care of their own health: qualitative study

Abstract Objective: To explore general practitioners' perceptions of the effects of their profession and training on their attitudes to illness in themselves and colleagues. Design: Qualitative study using focus groups and indepth interviews. Setting: Primary care in Northern Ireland. Participants: 27 general practitioners, including six recently appointed principals and six who also practised occupational medicine part time. Main outcome measures: Participants' views about their own and colleagues' health. Results: Participants were concerned about the current level of illness within the profession. They described their need to portray a healthy image to both patients and colleagues. This hindered acknowledgement of personal illness and engaging in health screening. Embarrassment in adopting the role of a patient and concerns about confidentiality also influenced their reactions to personal illness. Doctors' attitudes can impede their access to appropriate health care for themselves, their families, and their colleagues. A sense of conscience towards patients and colleagues and the working arrangements of the practice were cited as reasons for working through illness and expecting colleagues to do likewise. Conclusions: General practitioners perceive that their professional position and training adversely influence their attitudes to illness in themselves and their colleagues. Organisational changes within general practice, including revalidation, must take account of barriers experienced by general practitioners in accessing health care. Medical education and culture should strive to promote appropriate self care among doctors. What is already known on this topic High levels of stress, psychological distress, and suicide have been reported among doctors Doctors are reluctant to seek help in the normal way when they become stressed or ill What this study adds The perceived need to portray an unrealistically healthy image is stressful and a barrier to appropriate self care The emotional response to personal illness can produce an oscillation between panic and denial The working arrangements of general practitioners reinforce a culture in which their own and colleagues' distress is overlooked
Feature identification: a novel approach and a case study

Feature identification is a well-known technique to identify subsets of a program source code activated when exercising a functionality. Several approaches have been proposed to identify features. We present an approach to feature identification and comparison for large object-oriented multi-threaded programs using both static and dynamic data. We use processor emulation, knowledge filtering, and probabilistic ranking to overcome the difficulties of collecting dynamic data, i.e., imprecision and noise. We use model transformations to compare and to visualise identified features. We compare our approach with a naive approach and a concept analysis-based approach using a case study on a real-life large object-oriented multi-threaded program, Mozilla, to show the advantages of our approach. We also use the case study to compare processor emulation with statistical profiling.
Towards objective measures of speech intelligibility for Cochlear Implant users in reverberant environments

This study validates a novel approach to predict speech intelligibility for Cochlear Implant users (CIs) in reverberant environments. More specifically, we explore the use of existing objective quality and intelligibility metrics, applied directly to vocoded speech degraded by room reverberation, here assessed at ten different reverberation time (RT60) values: 0 s, 0.4 s - 1.0 s (0.1 s increments), 1.5 s and 2 s. Eight objective speech intelligibility predictors (SIPs) were investigated in this study. Of these, two were non-intrusive (i.e. did not require a reference signal) audio quality measures, four were intrusive, and two were intrusive speech intelligibility indexes. Three types of vocoders were implemented to examine how speech intelligibility predictions depended on the vocoder type. These were: noise-excited vocoder, tone-excited vocoder and a FFT-based N-of-M vocoder. Experimental results show that several intrusive quality and intelligibility measures were highly correlated with exponentially fit CI intelligibility data. On the other hand, only a recently - developed non-intrusive measure showed high correlations. These evaluations suggest that CI intelligibility may be accurately assessed via objective metrics applied to vocoded speech, thus may reduce the need for expensive and time-consuming listening tests.
A novel mutation in MIP associated with congenital nuclear cataract in a Chinese family

Purpose To identify the underlying genetic defect in a Chinese family affected with autosomal dominant congenital nuclear cataract. Methods A four-generation Chinese family with inherited nuclear cataract phenotype was recruited. Detailed family history and clinical data were recorded. All reported nuclear cataract-related candidate genes were screened for causative mutations by direct DNA sequencing. Effects of amino acid changes on the structure and function of protein were predicted by bioinformatics analysis. Results All affected individuals in this family showed nuclear cataracts. Sequencing of the candidate genes revealed a heterozygous c.559C>T change in the coding region of the major intrinsic protein (MIP), which caused a substitution of highly conserved arginine by cysteine at codon 187 (p.R187C). This mutation co-segregated with all affected individuals and was not observed in unaffected family members or 110 ethnically matched controls. Bioinformatics analysis showed that the mutation was predicted to affect the function and secondary structure of MIP protein. Conclusions This study identified a novel disease-causing mutation p.R187C in MIP in a Chinese cataract family, expanding the mutation spectrum of MIP causing congenital cataract.
ANTI-APOPTOTIC AND ANTI-INFLAMMATORY EFFECTS OF HYDROGEN SULFIDE IN A RAT MODEL OF REGIONAL MYOCARDIAL I/R

Hydrogen sulfide (H2S) is a novel gaseous mediator produced by cystathionine-&bgr;-synthase and cystathionine-&ggr;-lyase in the cardiovascular system, including the heart. Using a rat model of regional myocardial ischemia/reperfusion, we investigated the effects of an H2S donor (sodium hydrogen sulfide [NaHS]) on the infarct size and apoptosis caused by ischemia (25 min) and reperfusion (2 h). Furthermore, we investigated the potential mechanism(s) of the cardioprotective effect(s) afforded by NaHS. Specifically, we demonstrate that NaHS (1) attenuates the increase in caspase 9 activity observed in cardiac myocytes isolated from the area at risk (AAR) of hearts subjected in vivo to regional myocardial I/R and (2) ameliorates the decrease in expression of Bcl-2 within the AAR obtained from rat hearts subjected to regional myocardial I/R. The cardioprotective effects of NaHS were abolished by 5-hydroxydeconoate, a putative mitochondrial adenosine triphosphate-sensitive potassium channel blocker. Furthermore, NaHS attenuated the increase in the I/R-induced (1) phosphorylation of p38 mitogen-activated protein kinase and Jun N-terminal kinase, (2) translocation from the cytosol to the nucleus of the p65 subunit of nuclear factor-&kgr;B, (3) intercellular adhesion molecule 1 expression, (4) polymorphonuclear leukocyte accumulation, (5) myeloperoxidase activity, (6) malondialdehyde levels, and (7) nitrotyrosine staining determined in the AAR obtained from rat hearts subjected to regional myocardial I/R. In conclusion, we demonstrate that the cardioprotective effect of NaHS is secondary to a combination of antiapoptotic and anti-inflammatory effects. The antiapoptotic effect of NaHS may be in part due to the opening of the putative mitochondrial adenosine triphosphate-sensitive potassium channels.
The Crust of the Moon as Seen by GRAIL

The Holy GRAIL? The gravity field of a planet provides a view of its interior and thermal history by revealing areas of different density. GRAIL, a pair of satellites that act as a highly sensitive gravimeter, began mapping the Moon's gravity in early 2012. Three papers highlight some of the results from the primary mission. Zuber et al. (p. 668, published online 6 December) discuss the overall gravity field, which reveals several new tectonic and geologic features of the Moon. Impacts have worked to homogenize the density structure of the Moon's upper crust while fracturing it extensively. Wieczorek et al. (p. 671, published online 6 December) show that the upper crust is 35 to 40 kilometers thick and less dense—and thus more porous—than previously thought. Finally, Andrews-Hanna et al. (p. 675, published online 6 December) show that the crust is cut by widespread magmatic dikes that may reflect a period of expansion early in the Moon's history. The Moon's gravity field shows that the lunar crust is less dense and more porous than was thought. High-resolution gravity data obtained from the dual Gravity Recovery and Interior Laboratory (GRAIL) spacecraft show that the bulk density of the Moon's highlands crust is 2550 kilograms per cubic meter, substantially lower than generally assumed. When combined with remote sensing and sample data, this density implies an average crustal porosity of 12% to depths of at least a few kilometers. Lateral variations in crustal porosity correlate with the largest impact basins, whereas lateral variations in crustal density correlate with crustal composition. The low-bulk crustal density allows construction of a global crustal thickness model that satisfies the Apollo seismic constraints, and with an average crustal thickness between 34 and 43 kilometers, the bulk refractory element composition of the Moon is not required to be enriched with respect to that of Earth.
The Potential Impact of Bystander Effects on Radiation Risks in a Mars Mission

Abstract Brenner, D. J. and Elliston, C. D. The Potential Impact of Bystander Effects on Radiation Risks in a Mars Mission. Radiat. Res. 156, 612–617 (2001). Densely ionizing (high-LET) galactic cosmic rays (GCR) contribute a significant component of the radiation risk in free space. Over a period of a few months—sufficient for the early stages of radiation carcinogenesis to occur—a significant proportion of cell nuclei will not be traversed. There is convincing evidence, at least in vitro, that irradiated cells can send out signals that can result in damage to nearby unirradiated cells. This observation can hold even when the unirradiated cells have been exposed to low doses of low-LET radiation. We discuss here a quantitative model based on the a formalism, an approach that incorporates radiobiological damage both from a bystander response to signals emitted by irradiated cells, and also from direct traversal of high-LET radiations through cell nuclei. The model produces results that are consistent with those of a series of studies of the bystander phenomenon using a high-LET microbeam, with the end point of in vitro oncogenic transformation. According to this picture, for exposure to high-LET particles such as galactic cosmic rays other than protons, the bystander effect is significant primarily at low fluences, i.e., exposures where there are significant numbers of untraversed cells. If the mechanisms postulated here were applicable in vivo, using a linear extrapolation of risks derived from studies using intermediate doses of high-LET radiation (where the contribution of the bystander effect may be negligible) to estimate risks at very low doses (where the bystander effect may be dominant) could underestimate the true risk from low doses of high-LET radiation. It would be highly premature simply to abandon current risk projections for high-LET, low-dose radiation; however, these considerations would suggest caution in applying results derived from experiments using high-LET radiation at fluences above ∼1 particle per nucleus to risk estimation for a Mars mission.