Miracle-dz/UniFAR-FTUs huggingface dataset:
Effectiveness of Space Spraying on the Transmission of Dengue/Dengue Hemorrhagic Fever (DF/DHF) in an Urban Area of Southern Thailand

Timely and extensive space spraying has been widely used to prevent the spread of dengue fever/dengue hemorrhagic fever (DF/DHF). Field evaluations on its effectiveness have been rarely reported. This study aimed to evaluate the timeliness, coverage, and effectiveness of space spraying for DF/DHF control using a geographic information system (GIS). Longitudinal monitoring of DF/DHF cases and spray activities in Songkhla municipality was done between May 2006 and April 2007. After a case was detected, subsequent cases occurring within a 100 meter radius of the index case's house and between 16–35 days of onset were considered as potential secondary cases. During the study period, 140 cases of DF/DHF were detected. Of these, 25 were identified as secondary infections from 20 index cases. Where a secondary infection occurred, the mean attack rate was 2.7 per 1,000 population. Two significant predictors for being a secondary case were both related to the house of the index case, namely, absence of window screens and being constructed with corrugated iron sheets. Our findings suggest that space spraying in the study area was inadequate and often failed to prevent secondary cases of DF/DHF. Control programs should target houses constructed with corrugated iron sheets.
Analysis of the factors influencing red clover (Trifolium pratense L., Fabaceae) isoflavone content

This paper summarizes published literature concerning different factors that could affect the content and composition of isoflavones in red clover ( Trifolium pratense L., Fabaceae). Influence of genotype, plant part, geographic origin, ontogeny and storage conditions of raw material are discussed. Statistical analysis of available data on the profile of isoflavones in red clover suggest that, in general, samples are grouped according to country of origin, indicating the importance of climate conditions. An increase in biosynthesis of isoflavones as a response to different abiotic factors is also considered. Knowledge about these factors is of great practical importance, since red clover is a medicinal plant widely used for production of dietary supplements, and producers aim to breed cultivars with high isoflavone concentrations.
The E-screen assay: a comparison of different MCF7 cell stocks.

MCF7 human breast cancer cells have been studied extensively as a model for hormonal effects on breast cancer cell growth and specific protein synthesis. Because the proliferative effect of natural estrogen is considered the hallmark of estrogen action, it was proposed that this property be used to determine whether a substance is an estrogen. The E-screen assay, developed for this purpose, is based on the ability of MCF7 cells to proliferate in the presence of estrogens. The aim of our study was to characterize the response of four MCF7 cell stocks (BUS, ATCC, BB, and BB104) and determine which of them performed best in the E-screen test. The four stocks assayed were distinguishable by their biological behavior. In the absence of estrogen, MCF7 BUS cells stopped proliferating and accumulated in the G0/G1 phase of the cell cycle; estrogen receptors increased, progesterone receptors decreased, and small amounts of pS2 protein were secreted. Of all the MCF7 stocks tested, MCF7 BUS cells showed the highest proliferative response to estradiol-17 beta: cell yields increased up to sixfold over those of nontreated cells in a 144-hr period. The differences between estrogen-supplemented and nonsupplemented MCF7 BUS cells were due mostly to G0/G1 proliferative arrest mediated by charcoal dextran-stripped serum. MCF7 BUS cell stocks and others showing a similar proliferative pattern should be chosen for use in the E-screen test, or whenever a proliferative effect of estrogen is to be demonstrated. ImagesFigure 1. AFigure 1. BFigure 1. CFigure 1. DFigure 2. AFigure 2. BFigure 2. CFigure 2. DFigure 3. AFigure 3. BFigure 4. AFigure 4. BFigure 5. AFigure 5. BFigure 5. CFigure 5. D
Use of MDMA and Other Illicit Drugs by Young Adult Males in Northern Spain

Aim: To measure the prevalence of 3,4-methylenedioxymethamphetamine (MDMA) and other drug use in young males entering compulsory military service in Asturias (northern Spain) and to define trends in MDMA use in this group during the period from 1995 to 1999. We also sought to determine whether MDMA users have distinct personality features or higher levels of sensation seeking. Methods: 3,634 conscripts [mean age (SD) = 20.19 years (2.52)] who entered military service during the period between 1995 and 1999 were evaluated using the World Health Organization (WHO) questionnaire for drug consumption, the Eysenck Personality Questionnaire-A (EPQ-A), and the Zuckerman Sensation Seeking Scale. Results and Conclusions: The prevalence of lifetime, previous year and previous month MDMA use among military recruits between 1995 and 1999 was 10.9, 7.8 and 4.5%, ranking fifth among illicit drugs ever used. Once individuals used MDMA for the first time, they were likely to use it again, with 71% of individuals who had ever used MDMA reporting that they had used it during the past year (ranking second only to hallucinogens), and 41% reporting having used it in the last month. Compared to recruits who had never used MDMA (but who may have used other illicit drugs), MDMA users had a more extensive drug abuse history. Recruits who had used MDMA during the year prior to study had significantly higher scores on the Neuroticism and Psychoticism Subscales of the EPQ-A, and reported higher levels of sensation seeking.
Can the TLR-4-Mediated Signaling Pathway Be “A Key Inflammatory Promoter for Sporadic TAA”?

Thoracic aorta shows with advancing age various changes and a progressive deterioration in structure and function. As a result, vascular remodeling (VR) and medial degeneration (MD) occur as pathological entities responsible principally for the sporadic TAA onset. Little is known about their genetic, molecular, and cellular mechanisms. Recent evidence is proposing the strong role of a chronic immune/inflammatory process in their evocation and progression. Thus, we evaluated the potential role of Toll like receptor- (TLR-) 4-mediated signaling pathway and its polymorphisms in sporadic TAA. Genetic, immunohistochemical, and biochemical analyses were assessed. Interestingly, the rs4986790 TLR4 polymorphism confers a higher susceptibility for sporadic TAA (OR = 14.4, P = 0.0008) and it represents, together with rs1799752 ACE, rs3918242 MMP-9, and rs2285053 MMP-2 SNPs, an independent sporadic TAA risk factor. In consistency with these data, a significant association was observed between their combined risk genotype and sporadic TAA. Cases bearing this risk genotype showed higher systemic inflammatory mediator levels, significant inflammatory/immune infiltrate, a typical MD phenotype, lower telomere length, and positive correlations with histopatological abnormalities, hypertension, smoking, and ageing. Thus, TLR4 pathway should seem to have a key role in sporadic TAA. It might represent a potential useful tool for preventing and monitoring sporadic TAA and developing personalized treatments.
Histological and histochemical study of the exocrine pancreas in Madras red sheep (Ovis aries).

Microanatomical studies on the pancreas of different post-natal age groups of Madras red sheep was carried out. Pancreas consisted of both exocrine and endocrine parts where former formed the major portion. In all the age groups, pancreas was covered by a thin capsule from which septa arose and divided the glands in lobes and lobules. Blood vessels and nerve fibres were seen in the interlobular connective tissue. The exocrine portion consisted of secretory units (acini) and components of duct system. The duct system of the exocrine pancreas comprised of intercalated, intralobular, interlobular and pancreatic ducts. PAS and alcian blue positive reaction was noticed in the columnar epithelium on interlobular and pancreatic ducts. Lipid droplets were noticed beneath the capsule and in the interlobular septa in all the age groups. Distinct metachromasia was seen in the mast cells of the periductal connective tissue of interlobular and pancreatic ducts in all the age groups. The exocrine portion of the pancreas showed positive result for the activity of acid phosphatase, alkaline phosphatase, amylase, carbonic anhydrase, cholinesterase, cytochrome oxidase, lipase, glucose-6-phosphatase and succinic dehydrogenase. Most of the enzymes showed an increase in the activity as the age of animal advanced.
Use of an automated pyrosequencing technique for confirmation of sickle cell disease

Background The diagnosis of sickle cell disease (SCD) is made by hemoglobin assays such as high-performance liquid chromatography (HPLC), isoelectric focusing and cellulose acetate or citrate agar electrophoresis. These assays are easy to perform and used in large-scale newborn screening in many countries. These tests however may not easily differentiate Sβ0 thalassemia from SS or identify other hemoglobin variants, and in this case, hemoglobin (HBB) gene sequencing may be necessary. Objectives To develop a high throughput DNA based confirmatory assay for SCD and to detect mutations in the HBB gene. Methods We developed an automated pyrosequencing technique (PyS) based on QIAGEN technology (Hilden, Germany) to detect homozygous or heterozygous hemoglobin S mutations as well as hemoglobin C mutations. The technique was tested on 2,748 samples from patients enrolled in a multi-center SCD cohort in Brazil. Patients were previously tested using HPLC to diagnose SCD as part of routine clinical care. Any subjects with discrepant results between HPLC and PyS or with heterozygous hemoglobin S detected had Sanger sequencing of the HBB gene. Results We identified 168 samples with discrepant results between HPLC and PyS and 100 with concordant HPLC and PyS= heterozygous S, which would suggest Sβ-thalassemia or other hemoglobin S variants. The PyS assay correctly identified 1906 (98.7%) of the 1930 HbSS and 628 (98.7%) of the 636 HbSC samples. Of the 179 remaining samples, PyS correctly indicated S heterozygosis in 165 (92.2%). Of the 165 heterozygous S samples confirmed by Sanger as consistent with Sβ thalassemia genotype, 84 samples were classified as Sβ0 thalassemia and 81 as Sβ+ thalassemia. The most frequent beta thalassemia mutations of Sβ0 and Sβ+ were HBB: c.118C>T (Gln40Stop) and HBB c.92 + 6T> C, respectively. Discussion The PyS proved to be satisfactory for large-scale confirmatory testing of hemoglobin mutation. Moreover, with this study we were able to describe the most common β+ and β0 mutations in SCD patients with Sβ-thalassemia in a large multi-institutional SCD cohort in Brazil.
Stealthy malware detection through vmm-based "out-of-the-box" semantic view reconstruction

An alarming trend in malware attacks is that they are armed with stealthy techniques to detect, evade, and subvert malware detection facilities of the victim. On the defensive side, a fundamental limitation of traditional host-based anti-malware systems is that they run inside the very hosts they are protecting ("in the box"), making them vulnerable to counter-detection and subversion by malware. To address this limitation, recent solutions based on virtual machine (VM) technologies advocate placing the malware detection facilities outside of the protected VM ("out of the box"). However, they gain tamper resistance at the cost of losing the native, semantic view of the host which is enjoyed by the "in the box" approach, thus leading to a technical challenge known as the semantic gap. In this paper, we present the design, implementation, and evaluation of VMwatcher - an "out-of-the-box" approach that overcomes the semantic gap challenge. A new technique called guest view casting is developed to systematically reconstruct internal semantic views (e.g., files, processes, and kernel modules) of a VM from the outside in a non-intrusive manner. Specifically, the new technique casts semantic definitions of guest OS data structures and functions on virtual machine monitor (VMM)-level VM states, so that the semantic view can be reconstructed. With the semantic gap bridged, we identify two unique malware detection capabilities: (1) view comparison-based malware detection and its demonstration in rootkit detection and (2) "out-of-the-box" deployment of host-based anti-malware software with improved detection accuracy and tamper-resistance. We have implemented a proof-of-concept prototype on both Linux and Windows platforms and our experimental results with real-world malware, including elusive kernel-level rootkits, demonstrate its practicality and effectiveness.
Alternative Splicing of Neuroligin Regulates the Rate of Presynaptic Differentiation

Neuroligins (NLGs) and Neurexins (NRXs) are important adhesion molecules that promote synapse formation. Multiple splice variants of NLG and NRX exist, but their specific functions are unclear. Here we report that a surrogate postsynaptic cell expressing full-length NLG-1 triggers slow presynaptic differentiation in a contacting axon. In contrast, a version of NLG-1, which lacks insert B (NLG-1ΔB), induces rapid presynaptic differentiation, reaching the rate seen at native neuronal synapses. We show that this acceleration is attributed to the removal of the N-linked glycosylation site within insert B. NLG-1ΔB also increases synaptic density at neuro-neuronal synapses more than does full-length NLG-1. Other postsynaptic adhesion proteins, such as N-cadherin, EphB2, and SynCAM-1, alone or in combination with full-length NLG-1, do not trigger fast differentiation, suggesting that rapid presynaptic differentiation depends on a unique interaction of NLG-1ΔB with axonal proteins. Indeed, we find that NLG-1ΔB recruits more axonal α-NRX. Our results suggest that the engagement of α-NRX is a key to rapid induction of synapses at new sites of axo-dendritic contact.
Simultaneous and Proportional Estimation of Hand Kinematics From EMG During Mirrored Movements at Multiple Degrees-of-Freedom

This paper proposes and tests on able-bodied subjects a control strategy that can be practically applied in unilateral transradial amputees for simultaneous and proportional control of multiple degrees-of-freedom (DOFs). We used artificial neural networks to estimate kinematics of the complex wrist/hand from high-density surface electromyography (EMG) signals of the contralateral limb during mirrored bilateral movements in free space. The movements tested involved the concurrent activation of wrist flexion/extension, radial/ulnar deviation, forearm pronation/supination, and hand closing. The accuracy in estimation was in the range 79%-88% (r2 index) for the four DOFs in six able-bodied subjects. Moreover, the estimation of the pronation/supination angle (wrist rotation) was influenced by the reduction in the number of EMG channels used for the estimation to a greater extent than the other DOFs. In conclusion, the proposed method and set-up provide a viable means for proportional and simultaneous control of multiple DOFs for hand prostheses.
Genomic Mutation in Lines of Arabidopsis thaliana Exposed to Ultraviolet-B Radiation

Studies that have attempted to estimate the rate of deleterious mutation have typically been conducted under low levels of ultraviolet-B (UV-B) radiation, a naturally occurring mutagen. We conducted experiments to test whether the inclusion of natural levels of UV-B radiation in mutation-accumulation (MA) experiments influences the rate and effects of mildly deleterious mutation in the plant Arabidopsis thaliana. Ten generations of MA proved insufficient to observe significant changes in means or among-line variances in experimental lines maintained either with or without supplemental UV-B radiation. Maximum-likelihood estimates of mutation rate for total flower number revealed a small but significant rate of mutation for MA lines propagated under supplemental UV-B exposure, but not for those in which supplemental UV-B was omitted. A fraction of the flower number mutations under UV-B (∼25–30%) are estimated to increase flower number. Results from the application of transposon display to plant materials obtained after MA, in both the presence and absence of supplemental UV-B, suggest that the average rate of transposition for the class I and II transposable elements (TEs) surveyed was no more than 10−4. Overall, the estimates of mutation parameters are qualitatively similar to what has been observed in other MA experiments with this species in which supplemental UV-B levels have not been used. As well, it appears that naturally occurring levels of UV-B do not lead to detectable increases in levels of transposable element activity.
Further studies on carrageenan‐induced pleurisy in rats

A study has been made of the exudate provoked by intrapleural carrageenan in rats. Various parameters have been measured including volume of exudate, numbers and types of cells. Pharmacological assay has shown the early release of histamine and 5HT into the exudate followed at later times by a release of prostaglandins, and a second release of histamine. The role of complement has been studied. Intrapleural injection of carrageenan after complement depletion produced a reduced migration of polymorphonuclear cells and also a reduction in volume of exudate. Prostaglandins remained unaffected by this treatment.
Robust fusion of dynamic shape and normal capture for high-quality reconstruction of time-varying geometry

This paper describes a new passive approach to capture time-varying scene geometry in large acquisition volumes from multi-view video. It can be applied to reconstruct complete moving models of human actors that feature even slightest dynamic geometry detail, such as wrinkles and folds in clothing, and that can be viewed from 360deg. Starting from multi-view video streams recorded under calibrated lighting, we first perform marker-less human motion capture based on a smooth template with no high-frequency surface detail. Subsequently, surface reflectance and time-varying normal fields are estimated based on the coarse template shape. The main contribution of this paper is a new statistical approach to solve the non-trivial problem of transforming the captured normal field that is defined over the smooth non-planar 3D template into true 3D displacements. Our spatio-temporal reconstruction method outputs displaced geometry that is accurate at each time step of video and temporally smooth, even if the input data are affected by noise.
Targeted advertising and consumer information ∗ Job Market Paper

Using ever-increasing amounts of data, firms are able to link the valuations of consumers with the information they possess about a product. We analyse the impact of that new ability on the advertising strategy of a monopolist. If there is a positive correlation between consumers’ valuations and their information then, in contrast to the literature, better targeting often reduces prices. A lower price does not necessarily lead to a higher consumer surplus: some high-valuation/high-information consumers may stop purchasing because they stop receiving ads. Because of the interplay between the targeting and the pricing strategies, consumer surplus and welfare may be increasing in the advertising cost. Finally, we highlight that the link between valuation and information poses new problems for the estimation of returns to advertising.
EXISTENCE OF SOLUTION FOR SYSTEM OF INTEGRAL EQUATIONS AND FUNCTIONAL EQUATIONS THROUGH NON-NEGATIVE FUNCTION WITH PROPERTY OF ONENESS

In this article, we define the notion of a non-negative function satisfying property of oneness. Utilizing this concept, certain unified fixed point results are established. Furthermore generalized φ -contractions mapping and some fixed point results in the framework of symmetric space are also presented. Some well-known results from the existence literature are obtained through our introduced setting. Results are substantiated by some innovative examples with the aid of visualization of surfaces. Moreover applications of obtained theory for the existence for the system of integral equations as well as for the system of functional equations occurred in dynamic programming are also presented.
Serotherapy of primary rat mammary carcinoma: inhibition by ethylenedinitrilotetraacetic acid but not by [ethylenebis(oxyethylenenitrilo)]tetraacetic acid.

We reported inhibition of growth of primary rat mammary carcinomas after infusions of tumor-bearer plasma absorbed with Protein A-Sepharose or inactivated CNBr Sepharose. Absorbed plasmas were depleted of the third component of complement (C3) (other complement components defined similarly) and C5 but not C1, C4, or C2. These results suggested that activation of the alternative pathway of complement might be involved in the observed antitumor effects. To test this concept sera were treated with ethylenedinitrilotetraacetic acid or [ethylenebis(oxyethylenenitrilo)]tetraacetic acid before absorption with Protein A-Sepharose. Ethylenedinitrilotetraacetic acid, by chelating calcium and magnesium, prevents activation of both the alternative and classical complement pathways. [Ethylenebis(oxyethylenenitrilo)]tetraacetic acid, by chelating calcium but not magnesium, permits activation of the alternative pathway but inhibits activation of the classical complement pathway. Sera in the presence or absence of chelating agent were absorbed with Protein A-Sepharose twice at room temperature. After absorption calcium was added to the sera. Rats were treated by i.v. injection of sera twice a week for 2 weeks. Measurements of tumor size were made weekly for 5-7 weeks and then tumor weight was determined. Groups were compared both for size of index and total tumors. The results can be summarized as follows: tumor-bearer sera before absorption did not inhibit the growth of rat primary mammary carcinomas; tumor-bearer sera after absorption with Protein A-Sepharose showed significant consumption of C3 and did inhibit tumor growth; tumor-bearer sera absorbed in the presence of ethylenedinitrilotetraacetic acid did not show a decrease in C3 functional activity and did not inhibit tumor growth; tumor-bearer sera absorbed in the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid did show a decrease in C3 functional activity and did inhibit tumor growth; sera from normal adult female rats after absorption with Protein A-Sepharose did inhibit tumor growth. The results are consistent with a role for the alternative pathway of complement in the inhibition of growth of rat primary mammary carcinomas observed after treatment with absorbed sera.