Miracle-dz/UniFAR-FTUs huggingface dataset:
Modification of Epstein-Barr virus replication by tunicamycin

The effect of tunicamycin, which inhibits N-linked glycosylation, on the replication of Epstein-Barr virus was examined. Tunicamycin markedly reduced the yield of virus from producing cells. At concentrations of 1 to 2 micrograms of tunicamycin per ml, there was a buildup of intracellular virus in P3HR1-Cl13 cells but not in MCUV5 cells; at a concentration of 5 micrograms of tunicamycin per ml in P3HR1-Cl13 cells, viral DNA synthesis was inhibited as well. Viral glycoproteins lacking N-linked sugars were apparently inserted into the cell membrane, and the small amount of virus made in the presence of drug was able to bind specifically to its receptor on B cells. However, the ability of the virus to induce immunoglobulin secretion by fresh human lymphocytes was impaired. This implies a role for viral glycoproteins in the penetration as well as the attachment of virus.
Experience Modulates the Effects of Histone Deacetylase Inhibitors on Gene and Protein Expression in the Hippocampus: Impaired Plasticity in Aging

The therapeutic potential of histone deacetylase inhibitor (HDACi) treatment has attracted considerable attention in the emerging area of cognitive neuroepigenetics. The possibility that ongoing cognitive experience importantly regulates the cell biological effects of HDACi administration, however, has not been systematically examined. In an initial experiment addressing this issue, we tested whether water maze training influences the gene expression response to acute systemic HDACi administration in the young adult rat hippocampus. Training powerfully modulated the response to HDACi treatment, increasing the total number of genes regulated to nearly 3000, including many not typically linked to neural plasticity, compared with <300 following HDACi administration alone. Although water maze training itself also regulated nearly 1800 genes, the specific mRNAs, gene networks, and biological pathways involved were largely distinct when the same experience was provided together with HDACi administration. Next, we tested whether the synaptic protein response to HDACi treatment is similarly dependent on recent cognitive experience, and whether this plasticity is altered in aged rats with memory impairment. Whereas synaptic protein labeling in the young hippocampus was selectively increased when HDACi administration was provided in conjunction with water maze training, combined treatment had no effect on synaptic proteins in the aged hippocampus. Our findings indicate that ongoing experience potently regulates the molecular consequences of HDACi treatment and that the interaction of recent cognitive experience with histone acetylation dynamics is disrupted in the aged hippocampus. SIGNIFICANCE STATEMENT The possibility that interventions targeting epigenetic regulation could be effective in treating a range of neurodegenerative disorders has attracted considerable interest. Here we demonstrate in the rat hippocampus that ongoing experience powerfully modifies the molecular response to one such intervention, histone deacetylase inhibitor (HDACi) administration. A single learning episode dramatically shifts the gene expression profile induced by acute HDACi treatment, yielding a qualitatively distinct hippocampal transcriptome compared with the influence of behavioral training alone. The downstream synaptic protein response to HDACi administration is similarly experience-dependent, and we report that this plasticity is disrupted in the aged hippocampus. The findings highlight that accommodating the modulatory influence of ongoing experience represents a challenge for therapeutic development in the area of cognitive neuroepigenetics.
Fair scheduling with rate control for IEEE 802.16 broadband wireless networks

Although the scheduling problem of the uplink transmission in the IEEE 802.16 broadband wireless access network is extensively discussed, most of the results are limited to the quality of service (QoS) upon throughput and delay requirement. But as in practice only limited wireless resources are made available, a fairness-based scheduling upon each connection's QoS provides better outcomes. In this study, the authors propose a new fair uplink scheduling for real-time polling service and non-real-time polling service with the proportional sharing of excess bandwidth of the network. To implement the proposed fair scheduling that satisfies the delay requirement and full bandwidth utilisation, the authors introduce the rate control algorithm. With the proposed scheduling, we guarantee the fairness, delay requirement and full bandwidth utilisation which are not fully achieved in the existing results.
Gold(I)-Triphenylphosphine Complexes with Hypoxanthine-Derived Ligands: In Vitro Evaluations of Anticancer and Anti-Inflammatory Activities

A series of gold(I) complexes involving triphenylphosphine (PPh3) and one N-donor ligand derived from deprotonated mono- or disubstituted hypoxanthine (HLn) of the general composition [Au(Ln)(PPh3)] (1–9) is reported. The complexes were thoroughly characterized, including multinuclear high resolution NMR spectroscopy as well as single crystal X-ray analysis (for complexes 1 and 3). The complexes were screened for their in vitro cytotoxicity against human cancer cell lines MCF7 (breast carcinoma), HOS (osteosarcoma) and THP-1 (monocytic leukaemia), which identified the complexes 4–6 as the most promising representatives, who antiproliferative activity was further tested against A549 (lung adenocarcinoma), G-361 (melanoma), HeLa (cervical cancer), A2780 (ovarian carcinoma), A2780R (ovarian carcinoma resistant to cisplatin), 22Rv1 (prostate cancer) cell lines. Complexes 4–6 showed a significantly higher in vitro anticancer effect against the employed cancer cells, except for G-361, as compared with the commercially used anticancer drug cisplatin, with IC50 ≈ 1–30 µM. Anti-inflammatory activity was evaluated in vitro by the assessment of the ability of the complexes to modulate secretion of the pro-inflammatory cytokines, i.e. tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), in the lipopolysaccharide-activated macrophage-like THP-1 cell model. The results of this study identified the complexes as auspicious anti-inflammatory agents with similar or better activity as compared with the clinically applied gold-based antiarthritic drug Auranofin. In an effort to explore the possible mechanisms responsible for the biological effect, the products of interactions of selected complexes with sulfur-containing biomolecules (L-cysteine and reduced glutathione) were studied by means of the mass-spectrometry study.
Does Regulatory Harmonization Increase Bilateral Asset Holdings?

By combining new data on bilateral asset holdings with data on securities regulation in an empirical gravity model, it is found that bilateral differences in securities regulation lead to decreased portfolio holdings. Hence, regulatory harmonization can foster financial integration. The results are especially strong for equity holdings. It is verified that the results do not just reflect general economic, institutional, and cultural differences. Additional analysis of causality shows the exogenous component of asset holdings to be associated with larger differences in securities regulation. This might suggest that regulatory differences are used to protect domestic capital markets from outside competition.
Prelamin A impairs 53BP1 nuclear entry by mislocalizing NUP153 and disrupting the Ran gradient

The nuclear lamina is essential for the proper structure and organization of the nucleus. Deregulation of A‐type lamins can compromise genomic stability, alter chromatin organization and cause premature vascular aging. Here, we show that accumulation of the lamin A precursor, prelamin A, inhibits 53BP1 recruitment to sites of DNA damage and increases basal levels of DNA damage in aged vascular smooth muscle cells. We identify that this genome instability arises through defective nuclear import of 53BP1 as a consequence of abnormal topological arrangement of nucleoporin NUP153. We show for the first time that this nucleoporin is important for the nuclear localization of Ran and that the deregulated Ran gradient is likely to be compromising the nuclear import of 53BP1. Importantly, many of the defects associated with prelamin A expression were significantly reduced upon treatment with Remodelin, a small molecule recently reported to reverse deficiencies associated with abnormal nuclear lamina.
More Time, More Work: How Time Limits Bias Estimates of Project Duration and Scope

We propose that externally induced time limits overly affect predictions of other people’stask completion times, due to an over-generalized association between the time availableand inferred task scope. We find higher estimates of the time needed to complete a giventask by another person when the time limit is longer, even when the limit is unknown tothe other person, and therefore, cannot affect behavior. We propose that longer externaltime limits, even when irrelevant, trigger an over-learned perception that the taskinvolves more work. We provide evidence for this scope perception account in bothlaboratory experiments and field surveys and show that the account makes predictionsthat are distinct from an anchoring mechanism. We find that this over-learned cognitivebias persists even among experienced decision-makers making estimates in a familiarsetting, and is attenuated by weakening the association between time limits and taskscope. Our findings have implications for people who make decisions that use judgmentsof others’ task completion time as an input.
Cell cycle regulators p27 and pRb in lymphomas – correlation with histology and proliferative activity

The cell cycle is a complex event in which multiple regulator-proteins participate. The G1/S checkpoint of the cell cycle is controlled by pRb protein, which functions in its hypophosphorylated form as a negative regulator of growth. p27 (Kip1), a member of CIP/KIP family of cyclin inhibitory proteins, participates in inhibition of forming complexes that allow pRb to phosphorylate and lead the cell into mitosis. The expression of these important cell cycle regulator proteins was studied in a total of 96 non-Hodgkin's lymphoma (NHL) samples, which were classified according to the REAL classification. The expression of p27, pRb and the cell proliferation marker Ki-67 (MIB-1) was evaluated in lymphomas using immunohistochemistry. This study showed that there were coordinate changes in the expression of p27 and pRb in NHL. When compared to low-grade lymphomas, high-grade lymphomas showed significantly reduced expression of p27 and inversely pRb expression was increased (P < 0.001). Increase in expression of Ki-67 was parallel with pRb expression, and was mainly seen in cells that lacked p27 expression (P < 0.0001). This study suggests that changes in the control of the cell cycle closely relate to the pathobiology of NHL. © 2000 Cancer Research Campaign
Effects of elevated CO2 on predator avoidance behaviour by reef fishes is not altered by experimental test water

Pioneering studies into the effects of elevated CO2 on the behaviour of reef fishes often tested high-CO2 reared fish using control water in the test arena. While subsequent studies using rearing treatment water (control or high CO2) in the test arena have confirmed the effects of high CO2 on a range of reef fish behaviours, a further investigation into the use of different test water in the experimental arena is warranted. Here, we used a fully factorial design to test the effect of rearing treatment water (control or high CO2) and experimental test water (control or high CO2) on antipredator responses of larval reef fishes. We tested antipredator behaviour in larval clownfish Amphiprion percula and ambon damselfish Pomacentrus amboinensis, two species that have been used in previous high CO2 experiments. Specifically we tested if: 1) using control or high CO2 water in a two channel flume influenced the response of larval clownfish to predator odour, and 2) using control or high CO2 water in the test arena influenced the escape response of larval damselfish to a startle stimulus. Finally, 3) because the effects of high CO2 on fish behaviour appear to be caused by altered function of the GABA-A neurotransmitter we tested if antipredator behaviours were restored in clownfish treated with a GABA antagonist (gabazine) in high CO2 water. Larval clownfish reared from hatching in control water (496 μatm) strongly avoided predator cue whereas larval clownfish reared from hatching in high CO2 (1022 μatm) were attracted to the predator cue, as has been reported in previous studies. There was no effect of testing fish using control or high CO2 water in the flume. Larval damselfish reared for 4 days in high CO2 (1051 μatm) exhibited a slower response to a startle stimulus, slower escape speed and a shorter escape distance compared with fish reared in control conditions (464 μatm). There was no effect of test water on escape responses. Treatment of high-CO2 reared clownfish with 4 mg l−1 gabazine in high CO2 seawater restored the normal response to predator odour, as has been previously reported with fish tested in control water. Our results show that using control water in the experimental trials did not influence the results of previous studies on antipredator behaviour of reef fishes and also supports the results of novel experiments conducted in natural reef habitat at ambient CO2 levels.
Should the definition of preeclampsia include a rise in diastolic blood pressure of >/=15 mm Hg to a level <90 mm Hg in association with proteinuria?

OBJECTIVE This study was undertaken to compare baseline characteristics and pregnancy outcomes between normotensive women who did and those who did not have a rise in diastolic blood pressure of >/=15 mm Hg in association with proteinuria. STUDY DESIGN We studied 4302 healthy nulliparous women from the Calcium for Preeclampsia Prevention trial who were delivered at >/=20 weeks' gestation. We selected as the study group normotensive women who developed proteinuria within 7 days of a rise in diastolic blood pressure of >/=15 mm Hg with respect to baseline on 2 occasions 4 to 168 hours apart. Baseline blood pressure was the mean of measurements at 2 clinic visits before 22 weeks' gestation. Other normotensive women used for comparison were those who did not develop gestational hypertension or a rise in diastolic blood pressure of >/=15 mm Hg in association with proteinuria. RESULTS Except for greater weight (P <.001), body mass index (P <.001), and systolic blood pressure (P =.05) the baseline characteristics of the 82 women with a rise in diastolic blood pressure of >/=15 mm Hg in association with proteinuria did not differ significantly from those of the other normotensive women. Although they had a greater rate of weight gain (P <.005), larger babies (P =.06), and a 2-fold increase in abdominal delivery (P <.001), there was little other evidence of adverse pregnancy outcomes among these women. CONCLUSION During normotensive pregnancy a rise in diastolic blood pressure of >/=15 mm Hg in association with proteinuria appears to be benign and is not a useful clinical construct.
Insulin Sensitizing and Anti-Inflammatory Effects of Thiazolidinediones Are Heightened in Obese Patients

Objective The American Diabetes Association has called for further research on how patients’ demographics should determine drug choices for individuals with type 2 diabetes mellitus (T2DM). Here, using in-depth physiology studies, we investigate whether obese patients with T2DM are likely to benefit from thiazolidinediones, medications with a known adverse effect of weight gain. Materials and Methods Eleven obese and 7 nonobese individuals with T2DM participated in this randomized, placebo-controlled, double-blind, crossover study. Each subject underwent a pair of “stepped” pancreatic clamp studies with subcutaneous adipose tissue biopsies after 21 days of pioglitazone (45 mg) or placebo. Results Obese subjects demonstrated significant decreases in insulin resistance and many adipose inflammatory parameters with pioglitazone relative to placebo. Specifically, significant improvements in glucose infusion rates, suppression of hepatic glucose production, and whole fat expression of certain inflammatory markers (IL-6, IL-1B, and inducible nitric oxide synthase) were observed in the obese subjects but not in the nonobese subjects. Additionally, adipose tissue from the obese subjects demonstrated reduced infiltration of macrophages, dendritic cells, and neutrophils as well as increased expression of factors associated with fat “browning” (peroxisome proliferator–activated receptor gamma coactivator-1α and uncoupling protein-1). Conclusions These findings support the efficacy of pioglitazone to improve insulin resistance and reduce adipose tissue inflammation in obese patients with T2DM.
The Effects of Different Speed Training Protocols on Sprint Acceleration Kinematics and Muscle Strength and Power in Field Sport Athletes

Abstract Lockie, RG, Murphy, AJ, Schultz, AB, Knight, TJ, and Janse de Jonge, XAK. The effects of different speed training protocols on sprint acceleration kinematics and muscle strength and power in field sport athletes. J Strength Cond Res 26(6): 1539–1550, 2012—A variety of resistance training interventions are used to improve field sport acceleration (e.g., free sprinting, weights, plyometrics, resisted sprinting). The effects these protocols have on acceleration performance and components of sprint technique have not been clearly defined in the literature. This study assessed 4 common protocols (free sprint training [FST], weight training [WT], plyometric training [PT], and resisted sprint training [RST]) for changes in acceleration kinematics, power, and strength in field sport athletes. Thirty-five men were divided into 4 groups (FST: n = 9; WT: n = 8; PT: n = 9; RST: n = 9) matched for 10-m velocity. Training involved two 60-minute sessions per week for 6 weeks. After the interventions, paired-sample t-tests identified significant (p ⩽ 0.05) within-group changes. All the groups increased the 0- to 5-m and 0- to 10-m velocity by 9–10%. The WT and PT groups increased the 5- to 10-m velocity by approximately 10%. All the groups increased step length for all distance intervals. The FST group decreased 0- to 5-m flight time and step frequency in all intervals and increased 0- to 5-m and 0- to 10-m contact time. Power and strength adaptations were protocol specific. The FST group improved horizontal power as measured by a 5-bound test. The FST, PT, and RST groups all improved reactive strength index derived from a 40-cm drop jump, indicating enhanced muscle stretch-shortening capacity during rebound from impacts. The WT group increased absolute and relative strength measured by a 3-repetition maximum squat by approximately 15%. Step length was the major limiting sprint performance factor for the athletes in this study. Correctly administered, each training protocol can be effective in improving acceleration. To increase step length and improve acceleration, field sport athletes should develop specific horizontal and reactive power.
Internet addiction: Prevalence and risk factors: A cross-sectional study among college students in Bengaluru, the Silicon Valley of India.

BACKGROUND The Internet is a widely used tool known to foster addictive behavior, and Internet addiction threatens to develop into a major public health issue in the near future in a rapidly developing country like India. OBJECTIVE This cross-sectional study intends to estimate prevalence, understand patterns, and evaluate risk factors for Internet addiction among college students in the city of Bengaluru, India. MATERIALS AND METHODS Out of a total of 554 data samples from eight colleges selected through multistage cluster sampling, 515 samples were analyzed. Young's 20-item Internet Addiction Test (IAT), an inventory including demographic factors and patterns of internet use, was administered. RESULTS This study of college students aged 16-26 years (mean ± SD 19.2 ± 2.4 years), with marginally high female representation (56%), identified 34% [95% confidence interval (CI) 29.91-38.09%] and 8% (95%, CI 5.97-10.63%) as students with mild and moderate Internet addiction respectively. Binary logistic regression found Internet addiction to be associated with male gender [adjusted odds ratio (AOR) 1.69, 95% CI, 1.081- 2.65, P = 0.021], continuous availability online (AOR 1.724, 95% CI, 1.018-2.923, P = 0.042), using the Internet less for coursework/assignments (AOR 0.415, 95% CI, 0.263-0.655, P < 0.001), making new friendships online (AOR 1.721, 95% CI, 1.785-2.849, P = 0.034), getting into relationships online (AOR 2.283, 95% CI, 1.424-3.663, P = 0.001). CONCLUSION The results highlight the vulnerability of college students to Internet addiction. The findings provide explanations on the addictive behavior of the internet users, support the inclusion of "Internet Addiction" in the DSM-VI, and open up new paths for further research.
Method for Isolation of Bacteroides Bacteriophage Host Strains Suitable for Tracking Sources of Fecal Pollution in Water

ABSTRACT Bacteriophages infecting Bacteroides are potentially a good tool for fecal source tracking, but different Bacteroides host strains are needed for different geographic areas. A feasible method for isolating Bacteroides host strains for phages present in human fecal material is described. Useful strains were identified for application in Spain and the United Kingdom. One strain, GA-17, identified as Bacteroides thetaiotaomicron, was tested in several locations in Europe with excellent performance in Southern Europe.
Measurement of Internal Deformation of the Tongue during Vowel Sequence/ei/Using Tagged-Cine MRI

母音発話における外舌筋と内舌筋の機能を再評価するためタギングシネMRIと呼ばれる磁気共鳴画像法を用いて舌内組織の3次元的な変形を計測し, 母音/i/の生成にかかわる舌筋の役割を推定した. 中舌母音/e/を起点とする母音連鎖/ei/について矢状面2スライス-60フレーム/秒, 水平断面10スライス-12フレーム/秒の撮像を行った. 舌組織の3次元変形についてタグ陰影の分析を行った結果, /i/の調音において, 前半部の組織には前上方への移動および正中方向への圧縮, 後半部の組織には前下方向への移動および左右方向へ膨張が明らかになった. また舌組織の時間的な推移を比較した結果, 上半部は下半部に比べて移動量が大きく, 移動開始時期が早く, 移動速度も大きかった. 以上の結果は, 母音/i/の舌変形にオトガイ舌筋前部および後部筋束が関与するという従来の機構のみでは説明できず, 舌端部の組織を左右から正中方向に狭小化させ, 前上方へ向かう高速の変位をもたらす内舌筋 (主として横舌筋) の関与があることが推測された.
EGFR Mutations in Lung Cancer: Correlation with Clinical Response to Gefitinib Therapy

Receptor tyrosine kinase genes were sequenced in non–small cell lung cancer (NSCLC) and matched normal tissue. Somatic mutations of the epidermal growth factor receptor gene EGFR were found in 15of 58 unselected tumors from Japan and 1 of 61 from the United States. Treatment with the EGFR kinase inhibitor gefitinib (Iressa) causes tumor regression in some patients with NSCLC, more frequently in Japan. EGFR mutations were found in additional lung cancer samples from U.S. patients who responded to gefitinib therapy and in a lung adenocarcinoma cell line that was hypersensitive to growth inhibition by gefitinib, but not in gefitinib-insensitive tumors or cell lines. These results suggest that EGFR mutations may predict sensitivity to gefitinib.