Miracle-dz/UniFAR-FTUs huggingface dataset:
HLA-A*3101 and carbamazepine-induced hypersensitivity reactions in Europeans.

BACKGROUND Carbamazepine causes various forms of hypersensitivity reactions, ranging from maculopapular exanthema to severe blistering reactions. The HLA-B*1502 allele has been shown to be strongly correlated with carbamazepine-induced Stevens-Johnson syndrome and toxic epidermal necrolysis (SJS-TEN) in the Han Chinese and other Asian populations but not in European populations. METHODS We performed a genomewide association study of samples obtained from 22 subjects with carbamazepine-induced hypersensitivity syndrome, 43 subjects with carbamazepine-induced maculopapular exanthema, and 3987 control subjects, all of European descent. We tested for an association between disease and HLA alleles through proxy single-nucleotide polymorphisms and imputation, confirming associations by high-resolution sequence-based HLA typing. We replicated the associations in samples from 145 subjects with carbamazepine-induced hypersensitivity reactions. RESULTS The HLA-A*3101 allele, which has a prevalence of 2 to 5% in Northern European populations, was significantly associated with the hypersensitivity syndrome (P=3.5×10(-8)). An independent genomewide association study of samples from subjects with maculopapular exanthema also showed an association with the HLA-A*3101 allele (P=1.1×10(-6)). Follow-up genotyping confirmed the variant as a risk factor for the hypersensitivity syndrome (odds ratio, 12.41; 95% confidence interval [CI], 1.27 to 121.03), maculopapular exanthema (odds ratio, 8.33; 95% CI, 3.59 to 19.36), and SJS-TEN (odds ratio, 25.93; 95% CI, 4.93 to 116.18). CONCLUSIONS The presence of the HLA-A*3101 allele was associated with carbamazepine-induced hypersensitivity reactions among subjects of Northern European ancestry. The presence of the allele increased the risk from 5.0% to 26.0%, whereas its absence reduced the risk from 5.0% to 3.8%. (Funded by the U.K. Department of Health and others.).
The Usher gene cadherin 23 is expressed in the zebrafish brain and a subset of retinal amacrine cells

Purpose To characterize the expression pattern of cadherin 23 (cdh23) in the zebrafish visual system, and to determine whether zebrafish cdh23 mutants have retinal defects similar to those present in the human disease Usher syndrome 1D. Methods In situ hybridization and immunohistochemistry were used to characterize cdh23 expression in the zebrafish, and to evaluate cdh23 mutants for retinal degeneration. Visual function was assessed by measurement of the optokinetic response in cdh23 siblings and mutants. Results We detected cdh23 mRNA expression in multiple nuclei of both the developing and adult central nervous system. In the retina, cdh23 mRNA was expressed in a small subset of amacrine cells, beginning at 70 h postfertilization and continuing through adulthood. No expression was detected in photoreceptors. The cdh23-positive population of amacrine cells was GABAergic. Examination of homozygous larvae expressing two different mutant alleles of cdh23—cdh23tc317e or cdh23tj264a—revealed no detectable morphological retinal defects or degeneration. In addition, the optokinetic response to moving gratings of varied contrast or spatial frequency was normal in both mutants. Conclusions Unlike in other vertebrates, cdh23 is not detectable in zebrafish photoreceptors. Instead, cdh23 is expressed by a small subset of GABAergic amacrine cells. Moreover, larvae with mutations in cdh23 do not exhibit any signs of gross retinal degeneration or dysfunction. The role played by cdh23 in human retinal function is likely performed by either a different gene or an unidentified cdh23 splice variant in the retina that is not affected by the above mutations.
Contributions to the design and control of LCsCp resonant inverters to drive high-power HPS lamps

This paper presents the design criteria for full-bridge series-parallel (LC/sub s/C/sub p/) resonant inverters suitable for driving high-power high-intensity discharge lamps. By using the properties derived from the transfer functions of the inverter, a soft startup method is proposed. The lamp ignition is carried out maintaining the voltage and current variables below prefixed peak values, with the addition of no extra components to the power stage for this purpose. In steady-state operation, the proposed control minimizes the reactive voltamperes in the resonant tank. Moreover, the variation of the power delivered to the lamp, caused by the lamp aging, is limited in order to fulfil the standard. This design provides cost-effective circuits, simplifying the DC-AC power stage of an electronic ballast. The experimental results are given for high-pressure sodium lamps of the Sylvania SHP250W type.
In the Short Term We Divide, in the Long Term We Unite: Demographic Crisscrossing and the Effects of Faultlines on Subgroup Polarization

Do strong demographic faultlines breed opinion polarization in work teams? We integrate two theories that have been used to explain faultline effects. The first, the approach of Lau and Murnighan [Lau DC, Murnighan JK 1998 Demographic diversity and faultlines: The compositional dynamics of organizational groups. Acad. Management Rev. 232:325–340], suggests that in teams with strong faultlines the mechanisms of homophilous selection of interaction partners and persuasive influence cause subgroup polarization, defined as the split of the team into subgroups holding opposing opinions. The second, from sociological and anthropological traditions, emphasizes that crisscrossing actors bridge faultlines because they share demographic attributes with several subgroups. Demographically crisscrossing actors help to prevent polarization in social groups. We argue that Lau and Murnighan’s theory implicitly factors in the effects of crisscrossing actors. However, we show that the authors overlooked crucial implications of their theory because they did not consider crisscrossing actors explicitly. Most importantly, we demonstrate that demographic crisscrossing implies that even teams with strong faultlines will overcome polarization in the long run, although they might suffer from it in the short term. We develop and analyze a formal computational model of the opinion and network dynamics in work teams to show the consistency of our reasoning with Lau and Murnighans’ theory. The model also revealed another counterintuitive effect: strong faultlines lead to structures of interaction that make teams with strong faultlines faster in arriving at a stable consensus than teams with weak faultlines.
An iteratively weighted MMSE approach to distributed sum-utility maximization for a MIMO interfering broadcast channel

Consider the MIMO interfering broadcast channel whereby multiple base stations in a cellular network simultaneously transmit signals to a group of users in their own cells while causing interference to the users in other cells. The basic problem is to design linear beamformers that can maximize the system throughput. In this paper we propose a linear transceiver design algorithm for weighted sum-rate maximization that is based on iterative minimization of weighted mean squared error (MSE). The proposed algorithm only needs local channel knowledge and converges to a stationary point of the weighted sum-rate maximization problem. Furthermore, we extend the algorithm to a general class of utility functions and establish its convergence. The resulting algorithm can be implemented in a distributed asynchronous manner. The effectiveness of the proposed algorithm is validated by numerical experiments.
Implementation of a Wireless Mesh Network Testbed for Traffic Control

Wireless mesh networks (WMN) have attracted considerable interest in years as a convenient, flexible and low-cost alternative to wired communication infrastructures in many contexts. However, the great majority of research on metropolitan-scale WMN has been centered around maximization of available bandwidth, suitable for non-real-time applications such as Internet access for the general public. On the other hand, the suitability of WMN for mission-critical infrastructure applications remains by and large unknown, as protocols typically employed in WMN are, for the most part, not designed for real-time communications. In this paper, we describe the smart transport and roads communications (STaRComm) project at National ICT Australia (NICTA), which sets a goal of designing a wireless mesh network architecture to solve the communication needs of the traffic control system in Sydney, Australia. This system, known as SCATS (Sydney coordinated adaptive traffic system) and used in over 100 cities around the world, connects a hierarchy of several thousand devices - from individual traffic light controllers to regional computers and the central traffic management centre (TMC) - and places stringent requirements on the reliability and latency of the data exchanges. We discuss our experience in the deployment of an initial testbed consisting of 7 mesh nodes placed at intersections with traffic lights, and share the results and insights learned from our measurements and initial trials in the process.
Antibodies reacting with cytoplasm of subthalamic and caudate nuclei neurons in chorea and acute rheumatic fever

46% of sera from 30 children with rheumatic chorea showed IgG antibody reacting with neuronal cytoplasm of human caudate and subthalamic nuclei. The antibody was also detected in 14% of 50 children with active rheumatic carditis. 55 normal control sera, as well as 148 sera from a broad variety of other disease states showed a low prevalence (1.8-4.0%) of positive reactions. In rheumatic chorea the presence of anti-neuronal antibody appeared to correlate with severity and duration of clinical attacks. Antibody reacting with neuronal cytoplasm was completely removed by absorption with Group A streptococcal membranes or with isolated human neurons from caudate nucleus. Partial absorption of antibody was also recorded using Group A cell wall preparations but not with Group A carbohydrate. No absorption of positive reactions was seen with streptococcal Group D membranes or cell walls. In rheumatic chorea, anti-neuronal antibody appeared to represent cross-reaction with antigens shared by Group A streptococcal membranes.
In Vitro Quality Evaluation of 8 Days Storage of Random Donor Platelets Using Platelet Additive Solution

Background: The use of platelet additive solution (PAS) as plasma substitute during platelets (PLTs) storage could be an effective step in reducing plasma volume to decrease adverse events and prolonging PLT concentrate (PC) shelf life beyond the traditional 5 days. This study aimed at evaluating the applicability of using PAS as PLT storage media for extending PLT shelf life up to 8 days. Methods: 36 random donors (PCs) were prepared by platelet-rich plasma method and divided equally according to their storage media into: Group (1): 100% autologous plasma; Group (2): 30% autologous plasma and 70% PAS (SSP+); Group (3): 100% SSP+. All units were stored at 22 C on flatbed agitator for 8 days. PLT count and indices, metabolic variables, CD42b and CD62p expression were assessed on days 1, 5 and 8, besides automated BACTEC aerobic blood culture system for bacterial screening on days 1 and 8. Results: At 8 day storage, the mean PLT yield, mean PLT volume, PLT distribution width, swirling scores, metabolic parameters (glucose, LDH, PO2, and PCO2) levels showed best optimum values among group (2) units, compared to other groups. pH was maintained > 6.8 in all groups. The lowest CD62p expression and the highest CD42b expression were found among group (2), on days 1 and 8. No bacteriological growth was detected in all studied units. Conclusion: At 8 days storage, PCs using30% autologous plasma and 70% (SSP+) could provide the highest quality, particularly when assisted by a good and rapid bacterial detection system.
Genome-wide association study of clinically defined gout identifies multiple risk loci and its association with clinical subtypes

Objective Gout, caused by hyperuricaemia, is a multifactorial disease. Although genome-wide association studies (GWASs) of gout have been reported, they included self-reported gout cases in which clinical information was insufficient. Therefore, the relationship between genetic variation and clinical subtypes of gout remains unclear. Here, we first performed a GWAS of clinically defined gout cases only. Methods A GWAS was conducted with 945 patients with clinically defined gout and 1213 controls in a Japanese male population, followed by replication study of 1048 clinically defined cases and 1334 controls. Results Five gout susceptibility loci were identified at the genome-wide significance level (p<5.0×10−8), which contained well-known urate transporter genes (ABCG2 and SLC2A9) and additional genes: rs1260326 (p=1.9×10−12; OR=1.36) of GCKR (a gene for glucose and lipid metabolism), rs2188380 (p=1.6×10−23; OR=1.75) of MYL2-CUX2 (genes associated with cholesterol and diabetes mellitus) and rs4073582 (p=6.4×10−9; OR=1.66) of CNIH-2 (a gene for regulation of glutamate signalling). The latter two are identified as novel gout loci. Furthermore, among the identified single-nucleotide polymorphisms (SNPs), we demonstrated that the SNPs of ABCG2 and SLC2A9 were differentially associated with types of gout and clinical parameters underlying specific subtypes (renal underexcretion type and renal overload type). The effect of the risk allele of each SNP on clinical parameters showed significant linear relationships with the ratio of the case–control ORs for two distinct types of gout (r=0.96 [p=4.8×10−4] for urate clearance and r=0.96 [p=5.0×10−4] for urinary urate excretion). Conclusions Our findings provide clues to better understand the pathogenesis of gout and will be useful for development of companion diagnostics.
Constraints on Accretion in Ultraluminous X-Ray Sources from Spitzer IRS Observations of NGC 4485/4490: Infrared Diagnostic Diagrams

Constraining the astrophysical nature of ultraluminous X-ray (ULX) sources, which have X-ray luminosities exceeding 1039 ergs s-1, has been elusive due to the optical faintness of any counterparts. With high spectral resolution observations in the ~10-30 μm wavelength range we have conducted an experiment to study six ULX sources in the NGC 4485/4490 galaxy pair. We have found that five of the six ULXs, based on mid-infrared spectral diagnostics, show the characteristic higher ionization features that are found in AGNs. The sixth source, ULX-1, is consistent with being a supernova remnant. The chief infrared spectral diagnostics used are the ratios of [S III]/[Si II] versus [Ne III]/[Ne II]. In two instances fits to the continuum and polycyclic aromatic hydrocarbon (PAH) features also indicate higher dust temperatures, which are characteristic of accreting sources. Overall, however, we find that the continuum is dominated by stellar processes, and the best diagnostic features are the emission lines. High spectral resolution studies in the mid-infrared thus appear to show great promise for determining the astrophysical nature of ULXs.
Slowness: An Objective for Spike-Timing–Dependent Plasticity?

Our nervous system can efficiently recognize objects in spite of changes in contextual variables such as perspective or lighting conditions. Several lines of research have proposed that this ability for invariant recognition is learned by exploiting the fact that object identities typically vary more slowly in time than contextual variables or noise. Here, we study the question of how this “temporal stability” or “slowness” approach can be implemented within the limits of biologically realistic spike-based learning rules. We first show that slow feature analysis, an algorithm that is based on slowness, can be implemented in linear continuous model neurons by means of a modified Hebbian learning rule. This approach provides a link to the trace rule, which is another implementation of slowness learning. Then, we show analytically that for linear Poisson neurons, slowness learning can be implemented by spike-timing–dependent plasticity (STDP) with a specific learning window. By studying the learning dynamics of STDP, we show that for functional interpretations of STDP, it is not the learning window alone that is relevant but rather the convolution of the learning window with the postsynaptic potential. We then derive STDP learning windows that implement slow feature analysis and the “trace rule. ” The resulting learning windows are compatible with physiological data both in shape and timescale. Moreover, our analysis shows that the learning window can be split into two functionally different components that are sensitive to reversible and irreversible aspects of the input statistics, respectively. The theory indicates that irreversible input statistics are not in favor of stable weight distributions but may generate oscillatory weight dynamics. Our analysis offers a novel interpretation for the functional role of STDP in physiological neurons.
Diagnosing bacterial peritonitis made easy by use of leukocyte esterase dipsticks

Introduction: Spontaneous bacterial peritonitis (SBP) requires rapid diagnosis for the initiation of antibiotics. Its diagnosis is usually based on manual examination of ascitic fluid (AF) having long reporting time. AF infection is diagnosed when the fluid polymorphonuclear leukocyte (PMNL) concentration ≥250 cells/mm 3 . Aims and Objectives: Aim was to evaluate the diagnostic utility of leukocyte esterase (LE) reagent strip for rapid diagnosis of SBP in patients who underwent abdominal paracentesis and to calculate the sensitivity, specificity, positive, and negative predictive values. Materials and Methods: The study was carried out on 103 patients with ascites. Cell count of AF as determined by colorimetric scale of Multistix 10 SG reagent strip was compared with counting chamber method (PMNL count ≥250 cells/mm 3 was considered positive). Results and Observations: Of the 103 patients SBP was diagnosed in 20 patients, 83 patients were negative for SBP by manual cell count. The sensitivity and specificity of the LE test for detecting neutrocytic SBP taking grade 2 as cut off were 95% and 96.4% respectively, with a positive predictive value of 86.4% and a negative predictive value of 98.8%. Diagnostic accuracy of LE test was 96.1%. Discussion: There was a good correlation between the reagent strip result and PMNL count. The LE strip test is based on the esterase activity of activated granulocytes which reacts with an ester-releasing hydroxyphenylpyrrole causing a colour change in the azo dye of reagent strip. It is a very sensitive and specific method for the prompt detection of elevated PMNL count, and represents a convenient, inexpensive, simple, and bedside method for diagnosis of SBP. A negative LE test result excludes SBP with a high degree of certainty.
Using quadratic mean diameter and relative spacing index to enhance height-diameter and crown ratio models fitted to longitudinal data

The inclusion of quadratic mean diameter (QMD) and relative spacing index (RSI) substantially improved the predictive capacity of height–diameter at breast height (d.b.h.) and crown ratio models (CR), respectively. Data were obtained from 208 permanent plots established in western Arkansas and eastern Oklahoma during 1985–1987 andremeasuredforthesixthtime(2012–2014). Existingheight–d.b.h.andCRestimationmodelsfornaturallyoc-curringshortleafpineforests( Pinusechinata Mill.)wereupdatedandmodiﬁedforimprovedperformance. Addition-ally, eight height–d.b.h. relationship models that use only d.b.h. (fundamental local models) were modiﬁed using covariates.Themodelperformancewasevaluatedusing ﬁtstatistics[root meansquare error(RMSE), Fitindexand Akaike information criteria (AIC)]. The results showedthat the bestmodel form which was an extended non-linear modelwithautoregressiveﬁrstorderAR(1)structureandpowervariancefunctionperformedbetterthanextendedmixed-effectsmodelsandpredictedwellasanordinaryleastsquaresnon-linearmodel.Theautocorrelationwithinindividualtreeswaslargerfortheheight–d.b.h.relationshipthanforCRestimation. TheadditionofQMDtomeandominantheight( H with the use of basal area per hectare . Similarly, multiplying a fundamental local model by using QMD raised to a parametricpowerreducedRMSEby16percent,improvedFitindexby12percentanddecreasedtheAICvalueby7percent.D. b.h. , H D and RSI best explained the crown ratio relationship with an improved Fit index by 6.7 per cent comparedwithalternativenon-linearmodelswithoutRSI.ThelogisticmodelforCRalsoprovidedpredictionaccur-acysimilartothatofacommonlyusednon-linearmodel.Anon-linearmodelwithanapplicationofremedialmea-surestoenhanceadherencetomodellingassumptionscanprovidebetterparameterestimatesthanmixed-effectsmodellingapproach.
Female sterility in Ulmus minor (Ulmaceae): a hypothesis invoking the cost of sex in a clonal plant.

A high incidence of individuals with low seed set was found in two populations of the field elm Ulmus minor, a European tree that reproduces sexually and via vegetative propagation through root sprouting. One population was a seminatural stand, while the other was established by artificial propagation of genotypes sampled widely across Spain. The low seed set in both populations was due to both pre- and post-zygotic factors, the importance of which vary between genotypes. These factors included gynoecial malformations that produced a non-ovulated pistil, early gynoecial necrosis (i.e., necrosis before any opportunities for pollination), and seed abortion. Female sterility gave rise to two classes of individuals: trees that were largely female-sterile but dispersed normal quantities of viable pollen, and trees that dispersed both normal pollen and substantial numbers of seeds. Reduced production of protein-rich seeds may increase the resource availability for clonal propagation, helping to maintain female-sterile individuals with hermaphrodites.
Development of a microcontrolled bioinstrumentation system for active control of leg prostheses

This article describes the design of a microcontrolled bioinstrumentation system for active control of leg prostheses, using 4-channel electromyographic signal (EMG) detection and a single-channel electrogoniometer. The system is part of a control and instrumentation architecture in which a master processor controls the tasks of slave microcontrollers, through a RS-485 interface. Several signal processing methods are integrated in the system, for feature extraction (Recursive Least Squares), feature projection (Self Organizing Maps), and pattern classification (Levenberg-Marquardt Neural Network). The acquisition of EMG signals and additional mechanical information could help improving the precision in the control of leg prostheses.
Effects of a Motor and Multisensory-Based Approach on Residents with Moderate-to-Severe Dementia

Involving institutionalized people with dementia in their routines may be challenging, particularly in advanced stages of the disease. Motor and multisensory stimulation may help to maintain or improve residents’ remaining abilities such as communication and self-care. This study examines the effects of a motor and multisensory-based approach on the behavior of 6 residents with moderate-to-severe dementia. A single-group, pre- and post test design was conducted. Motor and multisensory stimulation strategies were implemented in residents’ morning care routines by staff, after the provision of training and assistance. Twelve video recordings of morning care (6 pre- and 6 post interventions) were coded for the type of residents’ behavior. Results showed a tendency toward improvements in residents’ levels of caregiver-direct gaze, laughing and engagement, and a reduction of closed eyes, during morning care. The introduction of a motor and multisensory-based approach in care routines may improve residents’ engagement and attention to the environment.