Miracle-dz/UniFAR-FTUs huggingface dataset:
AN ELECTRON MICROSCOPE STUDY OF RAT LEUKEMIA INDUCED WITH MOUSE LEUKEMIA VIRUS (GROSS).

Summary A comparative electron microscope study was carried out on ultrathin sections of bone marrow, spleen, lymph nodes, and mediastinal tumors from eighteen rats with leukemia induced by mouse leukemia virus (Gross). In organs of all eighteen rats with leukemia, two types of particles were observed: one type, described as virus particles of 990 A average diameter, with an electron-dense center of 630 A average diameter, centrally or excentrically located and surrounded by one or two membranes; the other type, described as vesicular or doughnut-type particles, with an electron-lucent center with an average diameter of 490 A, surrounded by an inner membrane and an outer membrane with an average diameter of 840 A. Occasionally, another membrane (580 A) could be seen between the inner and outer membranes of these particles. Both types of particles were found in the megakaryocytes of bone marrow and spleen, and the virus particles in the intercellular spaces in the lymph nodes, spleen, and in the mediastinal tumors. Occasionally, the particles were also found in cytoplasmic inclusions of cells present in the mediastinal tumors and in the lymph nodes. These particles were observed with equal frequency in these tissues, but apparently more frequently than in the spleen and bone marrow. Budding of plasma membrane of blast cells leading to particle formation was occasionally observed in the cells present in all organs examined. Similar budding of the membranes of cytoplasmic channels and of specific granules in the megakaryocytes of bone marrow and of spleen was also observed. Characteristic cylindrical or tubular structures were observed in the megakaryocytes of the bone marrow and spleen of leukemic rats. Segmentation of these structures into doughnut-type particles and budding into similar particles was also frequently seen. No particles of any type of cylindrical structures could be found in either bone marrow, spleen, thymus, or lymph nodes of fifteen normal rats.
Towards Automatic Identification of Discourse Markers in Dialogs: The Case of Like

This article discusses the detection of discourse markers (DM) in dialog transcriptions, by human annotators and by automated means. After a theoretical discussion of the definition of DMs and their relevance to natural language processing, we focus on the role of like as a DM. Results from experiments with human annotators show that detection of DMs is a difficult but reliable task, which requires prosodic information from soundtracks. Then, several types of features are defined for automatic disambiguation of like: collocations, part-of-speech tags and duration-based features. Decision-tree learning shows that for like, nearly 70% precision can be reached, with near 100% recall, mainly using collocation filters. Similar results hold for well, with about 91% precision at 100% recall.
Sharp error terms and neccessary conditions for exponential hitting times in mixing processes

We prove an upper bound for the error in the exponential approximation of the hitting time law of a rare event in α-mixing processes with exponential decay, ϕ-mixing processes with a summable function ϕ and for general ψ-mixing processes with a finite alphabet. In the first case the bound is uniform as a function of the measure of the event. In the last two cases the bound depends also on the time scale t. This allows us to get further statistical properties as the ratio convergence of the expected hitting time and the expected return time. A uniform bound is a consequence. We present an example that shows that this bound is sharp. We also prove that second moments are not necessary for having the exponential law. Moreover, we prove a necessary condition for having the exponential limit law.
Diet digestibility in growing rabbits: effect of origin and oxidation level of dietary fat and vitamin e supplementation.

The effects of the dietary inclusion of fats with different origin (lard or vegetal oil), fatty acid profile (linseed or sunflower), oxidation level (fresh, peroxidised: 11 d at 55oC or oxidised: 31 h at 140oC) and vitamin E supplementation (0 or 100 ppm) on the rabbit diet apparent digestibility were studied. Digestibility coefficients of dry matter, organic matter, crude protein, ether extract and gross energy were determined in eight diets using 58 rabbits aged 49 d. Contrast analysis between groups of diets showed that lard, characterised by a greater saturated fatty acid content, compared with vegetal oils, rich in unsaturated fatty acid, reduced the apparent digestibility of ether extract (62.3 vs. 68.4%; P=0.0329). However, there were no significant differences in the nutrient digestibility when linseed or sunflower oils (rich in ω-3 or in ω-6 polyunsaturated fatty acids, respectively) were compared. The oxidation degree of the sunflower oil and the supplementation with 100 ppm of vitamin E to the diets did not modify the apparent digestibility values of any dietary fraction. Key Words: rabbit, fat, ω-3/ω-6 fatty acids, oxidation level, tocopherol, digestibility.
Targeted capture and resequencing of 1040 genes reveal environmentally driven functional variation in grey wolves

In an era of ever‐increasing amounts of whole‐genome sequence data for individuals and populations, the utility of traditional single nucleotide polymorphisms (SNPs) array‐based genome scans is uncertain. We previously performed a SNP array‐based genome scan to identify candidate genes under selection in six distinct grey wolf (Canis lupus) ecotypes. Using this information, we designed a targeted capture array for 1040 genes, including all exons and flanking regions, as well as 5000 1‐kb nongenic neutral regions, and resequenced these regions in 107 wolves. Selection tests revealed striking patterns of variation within candidate genes relative to noncandidate regions and identified potentially functional variants related to local adaptation. We found 27% and 47% of candidate genes from the previous SNP array study had functional changes that were outliers in sweed and bayenv analyses, respectively. This result verifies the use of genomewide SNP surveys to tag genes that contain functional variants between populations. We highlight nonsynonymous variants in APOB, LIPG and USH2A that occur in functional domains of these proteins, and that demonstrate high correlation with precipitation seasonality and vegetation. We find Arctic and High Arctic wolf ecotypes have higher numbers of genes under selection, which highlight their conservation value and heightened threat due to climate change. This study demonstrates that combining genomewide genotyping arrays with large‐scale resequencing and environmental data provides a powerful approach to discern candidate functional variants in natural populations.
Return to play in elite rugby union: application of global positioning system technology in return-to-running programs.

UNLABELLED Global positioning systems (GPS) are widely used in sport settings to evaluate the physical demands on players in training and competition. The use of these systems in the design and implementation of rehabilitation and return-to-running programs has not yet been elucidated. OBJECTIVE To demonstrate the application of GPS technology in the management of return to play in elite-club Rugby Union. DESIGN Case series. SETTING Professional Rugby Union club team. PARTICIPANTS 8 elite Rugby Union players (age 27.86 ± 4.78 y, height 1.85 ± 0.08 m, weight 99.14 ± 9.96 kg). INTERVENTION Players wore GPS devices for the entire duration of a club game. MAIN OUTCOME MEASURES Variables of locomotion speed and distance were measured. RESULTS Differences in physical demands between playing positions were observed for all variables. CONCLUSIONS An analysis of the position-specific physical demands measured by GPS provides key information regarding the level and volume of loads sustained by a player in a game environment. Using this information, sports-medicine practitioners can develop rehabilitation and return-to-running protocols specific to the player position to optimize safe return to play.
Perceiving Vowels and Tones in Mandarin : The Effect of Literary Phonetic Systems on Phonological Awareness

A tone-vowel monitoring task similar to Ye and Connine’s (1999) experiment was conducted in Taiwan to examine how literary phonetic systems affect people’s perception of vowels, and then the relative temporal availability of vowel and tonal information. The results demonstrated that although participants were sensitive to the acoustic nature of monosyllablic stimuli, they mainly resorted to the literary systems they learned during the perception. Therefore, the impact of literary systems on the perception was taken into consideration when the relation of vowels and tones was investigated in this study. With the consideration, it was found that vowel information was available relatively earlier than the tonal information.
Synthesis of Silver Nanoparticles Using Buchu Plant Extracts and Their Analgesic Properties

We herein report for the first time the synthesis and analgesic properties of silver nanoparticles (Ag-NPs) using buchu plant extract. The as-synthesised Ag-NPs at different temperatures were characterised by UV-Vis spectroscopy, Fourier transform infra-red spectroscopy (FTIR) and transmission transform microscopy (TEM) to confirm the formation of silver nanoparticles. Phytochemical screening of the ethanolic extract revealed the presence of glycosides, proteins, tannins, alkaloids, flavonoids and saponins. The absorption spectra showed that the synthesis is temperature and time dependent. The TEM analysis showed that the as-synthesised Ag-NPs are polydispersed and spherical in shape with average particle diameter of 19.95 ± 7.76 nm while the FTIR results confirmed the reduction and capping of the as-synthesised Ag-NPs by the phytochemicals present in the ethanolic extract. The analgesic study indicated that the combined effect of the plant extract and Ag-NPs is more effective in pain management than both the aspirin drug and the extract alone.
Annual research review: re-thinking the classification of autism spectrum disorders.

BACKGROUND The nosology of autism spectrum disorders (ASD) is at a critical point in history as the field seeks to better define dimensions of social-communication deficits and restricted/repetitive behaviors on an individual level for both clinical and neurobiological purposes. These different dimensions also suggest an increasing need for quantitative measures that accurately map their differences, independent of developmental factors such as age, language level and IQ. METHOD Psychometric measures, clinical observation as well as genetic, neurobiological and physiological research from toddlers, children and adults with ASD are reviewed. RESULTS The question of how to conceptualize ASDs along dimensions versus categories is discussed within the nosology of autism and the proposed changes to the DSM-5 and ICD-11. Differences across development are incorporated into the new classification frameworks. CONCLUSIONS It is crucial to balance the needs of clinical practice in ASD diagnostic systems, with neurobiologically based theories that address the associations between social-communication and restricted/repetitive dimensions in individuals. Clarifying terminology, improving description of the core features of ASD and other dimensions that interact with them and providing more valid and reliable ways to quantify them, both for research and clinical purposes, will move forward both practice and science.
Ongoing outbreak of hepatitis A in Italy: preliminary report as of 31 May 2013.

Since January 2013, an unusual increase in hepatitis A cases has been detected in northern Italy. A total number of 352 cases were reported to the integrated surveillance system between January and the end of May 2013 and this represents a 70% increase compared to the same period of the previous year. The outbreak is ongoing and the public health authorities are continuing their investigations to establish the transmission vehicle and to control the outbreak.
Projections from the hippocampal region to the mammillary bodies in macaque monkeys

A combination of anterograde and retrograde tracers mapped the direct hippocampal and parahippocampal inputs to the mammillary bodies in two species of macaque monkey. Dense projections arose from pyramidal cells in layer III of the subiculum and prosubiculum, and terminated in the medial mammillary nucleus. While there was no evidence of an input from the dentate gyrus or fields CA1–3, a small contribution arose from the presubiculum and entorhinal cortices. All of the hippocampal and parahippocampal projections to the mammillary bodies appeared to use the fornix as a route. The caudal portions of the subiculum and prosubiculum contained the greatest numbers of cells projecting to the mammillary bodies. A light contralateral projection to the medial mammillary nucleus was also observed, although this appeared to arise primarily from the more rostral portions of the subiculum and prosubiculum. There was a crude topography within the medial mammillary nucleus, with the caudal subicular projections terminating in the mid and dorsal portions of the nucleus while the rostral subicular and entorhinal projections terminated in the ventral and lateral portions of the medial nucleus. Light ipsilateral projections throughout the lateral mammillary nucleus were sometimes observed. Comparisons with related studies of the macaque brain showed that the dense hippocampal projections to the mammillary bodies arise from a population of subicular cells separate from those that project to the anterior thalamic nuclei, even though the major output from the mammillary bodies is to the anterior thalamic nuclei. Other comparisons revealed underlying similarities with the corresponding projections in the rat brain.
A population-level metric for gambling-related harm

Abstract Prior estimates of the population-level impact of gambling have relied on economic costings. Recent work has derived disability weights for the Problem Gambling Severity Index, which measure per-person impact of gambling on quality of life on a scale of zero to one. This provided scope for the present study to calculate the ‘burden of gambling harm’, which captures the aggregate impact of harms arising from gambling on quality of life in a population. Gambling-related harm was associated with 101,675 years of life lost in Victoria, Australia: approximately two-thirds that of alcohol use and dependence, and major depressive disorder. Problem gamblers suffer more individually (disability weight = .44) compared to those in moderate (.29) and low (.13) risk categories. Nevertheless, moderate and low-risk gamblers account for 85% of population-level harm, due to greater prevalence of these groups. Overall, the scale of gambling-related harm is large relative to other significant health issues, with milder yet non-negligible harm accruing to a relatively broad segment of the gambling population. The article suggests that the tendency to conflate the (typically low) prevalence of problem gambling with total gambling impact is misleading, and argues for a broader population-health based measure.
Activation of Muscarinic Receptors in Rat Bladder Sensory Pathways Alters Reflex Bladder Activity

Antimuscarinic drugs affect bladder sensory symptoms such as urgency and frequency, presumably by acting on muscarinic acetylcholine receptors (mAChRs) located in bladder sensory pathways including primary afferent nerves and urothelium. However, the expression and the function of these receptors are not well understood. This study investigated the role of mAChRs in bladder sensory pathways in vivo in urethane anesthetized rats. Intravesical administration of the mAChR agonist oxotremorine methiodide (OxoM) elicited concentration-dependent excitatory and inhibitory effects on the frequency of voiding. These effects were blocked by intravesical administration of the mAChR antagonist atropine methyl nitrate (5 μm) and were absent in rats pretreated with capsaicin to desensitize C-fiber afferent nerves. Low concentrations of OxoM (5 μm) decreased voiding frequency by ∼30%, an effect blunted by inhibiting nitric oxide (NO) synthesis with l-NAME (Nω-nitro-l-arginine methyl ester hydrochloride; 5 mg/kg; i.v.). High concentrations of OxoM (40 μm) increased voiding frequency by ∼45%, an effect blunted by blocking purinergic receptors with PPADS (0.1–1 mm; intravesically). mAChR agonists stimulated release of ATP from cultured urothelial cells. Intravenous administration of OxoM (0.01–5 μg/kg) did not mimic the intravesical effects on voiding frequency. These results suggest that activation of mAChRs located near the luminal surface of the bladder affects voiding functions via mechanisms involving ATP and NO release presumably from the urothelium, that in turn could act on bladder C-fiber afferent nerves to alter their firing properties. These findings suggest that the urothelial-afferent nerve interactions can influence reflex voiding function.
Chickadee behavioural response to varying threat levels of predator and conspecific calls

Chickadees produce many vocalizations, including chick-a-dee calls which they use as a mobbing call in the presence of predators. Previous research has shown that chickadees produce more D notes in their mobbing calls in response to high-threat predators compared to low-threat predators, and may perceive predator and corresponding mobbing vocalizations as similar. We presented black-capped chickadees with playback of high- and low-threat predator calls and conspecific mobbing calls, and non-threat heterospecific and reversed mobbing calls, to examine vocal and movement behavioural responses. Chickadees produced more chick-a-dee calls in response to playback of calls produced by a high-threat predator compared to calls produced by a low-threat predator, and to reversed high-threat mobbing calls compared to normal (i.e., non-reversed) high-threat mobbing calls. Chickadees also vocalized more in response to all playback conditions consisting of conspecific mobbing calls compared to a silent baseline period. The number of D notes that the subjects produced was similar to previous findings; chickadees produced approximately one to three D notes per call in response to low-threat mobbing calls, and produced more calls containing four to five D notes in response to high-threat mobbing calls, although this difference in the number of D notes per call was not significant. The difference in chickadees’ production of tseet calls across playback conditions approached significance as chickadees called more in response to conspecific mobbing calls, but not in response to heterospecific calls. General movement activity decreased in response to playback of conspecific-produced vocalizations, but increased in response to heterospecific-produced vocalizations, suggesting that chickadees may mobilize more in response to predator playback in preparation for a “fight or flight” situation. These results also suggest that chickadees may produce more mobbing calls in response to high-threat predator vocalizations as an attempt to initiate mobbing with conspecifics, while they produce fewer mobbing calls in response to a low-threat predator that a chickadee could outmaneuver.
Mental health profiles and quality of life among Korean adolescents

In an attempt to identify and intervene with students in need of services, the South Korean government has implemented national mental health screening. However, concerns raised about the unintended stigmatization of the screening assessment that focuses on student deficits prompts the need for additional research. This study evaluated the potential utility of an alternative screening approach that considers student strengths, in addition to symptoms of distress. Using a sample of 1,190 Korean adolescents enrolled in grades seven to nine, two latent profile analyses were conducted to identify underlying mental health strength and distress subtypes. Results identified five subtypes of psychological strengths and three subtypes of psychological distress. As hypothesized, students with higher levels of strengths and lower levels of distress reported better quality of life, academic performance, and higher life satisfaction. Implications for school based mental health screening and future directions for researchers and practitioners are discussed.
HCV Kinetic Models and Their Implications in Drug Development

Chronic infection with hepatitis C virus (HCV) affects about 170 million people worldwide and is a major cause of liver complications. Mathematical modeling of viral kinetics under treatment has provided insight into the viral life cycle, treatment effectiveness, and drugs' mechanisms of action. Here we review the implications of viral kinetic models at the different stages of development of anti‐HCV agents.
Perceptions of transformational leadership and job satisfaction: The roles of personality traits and psychological empowerment §

Abstract Through two studies, this paper investigates the moderating effects of personality traits (i.e., extraversion, conscientiousness, agreeableness and neuroticism) and the mediating effect of psychological empowerment on the relationship between perceived transformational leadership and job satisfaction. Study 1 searches whether personality traits moderate the effects of perceived transformational leadership on followers’ job satisfaction. Using a sample of 221 R&D employees employed by information technology organizations, the results of Study 1 indicate that the more conscientious the employee, the stronger the relationship between perceived transformational leadership and job satisfaction. Study 2 explores whether psychological empowerment mediates the effects of perceived transformational leadership on followers’ job satisfaction. Based on data from 348 academics, the results support the mediating role of psychological empowerment on job satisfaction, in that when employees perceive their leader as transformational they feel more psychologically empowered, which in turn increases job satisfaction levels. Implications for future research and practice are discussed.
Making a Short Gamma-Ray Burst from a Long One: Implications for the Nature of GRB 060614

The absence of a supernova accompanying the nearby long GRB 060614 poses a great puzzle about the progenitor of this event and challenges the current GRB classification scheme. This burst displays a short-hard emission episode followed by extended soft emission with strong spectral evolution. Noticing that this burst has an isotropic gamma-ray energy only ~8 times that of GRB 050724, a good candidate of merger-type short GRBs, we generate a "pseudo"burst that is ~8 times less energetic than GRB 060614 based on the spectral properties of GRB 060614 and the Ep ∝ E (Amati) relation. We find that this pseudoburst would have been detected by BATSE as a marginal short-duration GRB and would have properties in the Swift BAT and XRT bands similar to GRB 050724. This suggests that GRB 060614 is likely a more intense event in the traditional short-hard GRB category. Events like GRB 060614 that seem to defy the traditional short versus long classification of GRBs may require modification of our classification terminology for GRBs. By analogy with supernova classifications, we suggest that GRBs be classified into Type I (typically short and associated with old populations) and Type II (typically long and associated with young populations). We propose that GRB 060614 belongs to Type I and predict that similar events will be detected in elliptical galaxies.