Miracle-dz/UniFAR-FTUs huggingface dataset:
IDH1 Mutations as Molecular Signature and Predictive Factor of Secondary Glioblastomas

Purpose: To establish the frequency of IDH1 mutations in glioblastomas at a population level, and to assess whether they allow reliable discrimination between primary (de novo) glioblastomas and secondary glioblastomas that progressed from low-grade or anaplastic astrocytoma. Experimental Design: We screened glioblastomas from a population-based study for IDH1 mutations and correlated them with clinical data and other genetic alterations. Results: IDH1 mutations were detected in 36 of 407 glioblastomas (8.8%). Glioblastoma patients with IDH1 mutations were younger (mean, 47.9 years) than those with EGFR amplification (60.9 years) and were associated with significantly longer survival (mean, 27.1 versus 11.3 months; P < 0.0001). IDH1 mutations were frequent in glioblastomas diagnosed as secondary (22 of 30; 73%), but rare in primary glioblastomas (14 of 377; 3.7%: P < 0.0001). IDH1 mutations as genetic marker of secondary glioblastoma corresponded to the respective clinical diagnosis in 95% of cases. Glioblastomas with IDH1 mutation diagnosed as primary had clinical and genetic profiles similar to those of secondary glioblastomas, suggesting that they may have rapidly progressed from a less malignant precursor lesion that escaped clinical diagnosis and were thus misclassified as primary. Conversely, glioblastomas without IDH1 mutations clinically diagnosed as secondary typically developed from anaplastic rather than low-grade gliomas, suggesting that at least some were actually primary glioblastomas, that may have been misclassified, possibly due to histologic sampling error. Conclusion: IDH1 mutations are a strong predictor of a more favorable prognosis and a highly selective molecular marker of secondary glioblastomas that complements clinical criteria for distinguishing them from primary glioblastomas. (Clin Cancer Res 2009;15(19):6002–7)
PUBLIC COOPERATION WITH THE POLICE IN GHANA: DOES PROCEDURAL FAIRNESS MATTER?*

Recent criminological emphasis on the salience of normative concerns, such as procedural fairness and legitimacy, in understanding public law-abiding behavior has been based on evidence from Anglo-American studies. This article examines these issues in the African context based on general survey data from Accra, Ghana. The results show a lack of empirical validity, in the Ghanaian context, of the Sunshine–Tyler legitimacy scale. The results also show that public cooperation with the police in Ghana is shaped by utilitarian factors such as perceptions of current police effectiveness infighting crime. It is argued that the importance of perceived police effectiveness to public cooperation is a result of police legitimation deficits and the public's alienation from the Ghana police, which in turn are traced to the colonial history of the police and current poor police performance.
Effect of Arylamine Acetyltransferase Nat3 Gene Knockout on N-Acetylation in the Mouse

Arylamine N-acetyltransferases (NAT) catalyze the biotransformation of many important arylamine drugs and procarcinogens. NAT can either detoxify or activate procarcinogens, complicating the manner in which these enzymes may participate in enhancing or preventing toxic responses to particular agents. Mice possess three NAT isoenzymes: Nat1, Nat2, and Nat3. Whereas Nat1 and Nat2 can efficiently acetylate many arylamines, few substrates appear to be appreciably metabolized by Nat3. We generated a Nat3 knockout mouse strain and used it along with our double Nat1/2(–/–) knockout strain to further investigate the functional role of Nat3. Nat3(–/–) mice showed normal viability and reproductive capacity. Nat3 expression was very low in wild-type animals and completely undetectable in Nat3(–/–) mice. In contrast, greatly elevated expression of Nat3 transcript was observed in Nat1/2(–/–) mice. We used a transcribed marker polymorphism approach to establish that the increased expression of Nat3 in Nat1/2(–/–) mice is a positional artifact of insertion of the phosphoglycerate kinase-neomycin resistance cassette in place of the Nat1/Nat2 gene region and upstream of the intact Nat3 gene, rather than a biological compensatory mechanism. Despite the increase in Nat3 transcript, the N-acetylation of p-aminosalicylate, sulfamethazine, 2-aminofluorene, and 4-aminobiphenyl was undetectable either in vivo or in vitro in Nat1/2(–/–) animals. In parallel, no difference was observed in the in vivo clearance or in vitro metabolism of any of these substrates between wild-type and Nat3(–/–) mice. Thus, Nat3 is unlikely to play a significant role in the N-acetylation of arylamines either in wild-type mice or in mice lacking Nat1 and Nat2 activities.
Surveillance of Influenza A Virus in Wild Birds in the Asian Portion of Russia in 2008

SUMMARY. Wild waterfowl undertake a variety of long-distance flights during their migration. These flights provide birds with the opportunities to both acquire and disseminate avian influenza viruses (AIVs). The Asian portion of Russia is crossed by four major migration routes and represents the major breeding area for many wild bird species in the Palearctic. The Asian territory of Russia plays an important role in distribution, persistence, and evolution of AIVs due to the ecologic relationships of bird populations from Russia and different Asian, European, African, and North American countries. Our study highlights the results of surveillance conducted in 2008 for AIVs in wild birds in the Asian portion of Russia. During this study, our team collected and tested 5678 samples from wild birds. Among them, 41 samples tested positive for AIV with an isolation rate of 0.72%. The highest AIV prevalence, 1.49%, was found in Anseriformes. In Ardeidae and Laridae, the AIV prevalence was 1.23% and 0.64%, respectively. Rallidae showed the lowest AIV prevalence of 0.61%. Phylogenetic analysis of H3 and H4 subtypes represented close relationships of AIVs isolated from the Asian portion of Russia to the AI strains from Asia, Africa, and Europe. These findings were confirmed by the wild bird migration routes that affect bird populations from Eurasian, African, Australian, and North American continents.
Combined Cocaine Hydrolase Gene Transfer and Anti-Cocaine Vaccine Synergistically Block Cocaine-Induced Locomotion

Mice and rats were tested for reduced sensitivity to cocaine-induced hyper-locomotion after pretreatment with anti-cocaine antibody or cocaine hydrolase (CocH) derived from human butyrylcholinesterase (BChE). In Balb/c mice, direct i.p. injection of CocH protein (1 mg/kg) had no effect on spontaneous locomotion, but it suppressed responses to i.p. cocaine up to 80 mg/kg. When CocH was injected i.p. along with a murine cocaine antiserum that also did not affect spontaneous locomotion, there was no response to any cocaine dose. This suppression of locomotor activity required active enzyme, as it was lost after pretreatment with iso-OMPA, a selective BChE inhibitor. Comparable results were obtained in rats that developed high levels of CocH by gene transfer with helper-dependent adenoviral vector, and/or high levels of anti-cocaine antibody by vaccination with norcocaine hapten conjugated to keyhole limpet hemocyanin (KLH). After these treatments, rats were subjected to a locomotor sensitization paradigm involving a “training phase" with an initial i.p. saline injection on day 1 followed by 8 days of repeated cocaine injections (10 mg/kg, i.p.). A 15-day rest period then ensued, followed by a final “challenge" cocaine injection. As in mice, the individual treatment interventions reduced cocaine-stimulated hyperactivity to a modest extent, while combined treatment produced a greater reduction during all phases of testing compared to control rats (with only saline pretreatment). Overall, the present results strongly support the view that anti-cocaine vaccine and cocaine hydrolase vector treatments together provide enhanced protection against the stimulatory actions of cocaine in rodents. A similar combination therapy in human cocaine users might provide a robust therapy to help maintain abstinence.
Portfolio Choice and Risk Attitudes: An Experiment

We study the following basic intuition: when faced with a decision how to split their investment between a risky lottery and an asset with a fixed return, people increase the proportion invested in the risky option the more they like the lottery. We find counter-examples to this, and in fact we find no simple relation between preferences between lotteries and the fraction invested in them. We use three well-documented biases (ambiguity aversion, the illusion of control and myopic loss aversion) to show this. First we replicate the previous results in a laboratory experiment with financial incentives, and then test whether participants are willing to explicitly pay a small sum of money in line with the bias (pay for less ambiguity, more perceived control, or more frequent information about portfolio performance). We then study how portfolio choice depends on these biases. With the parameters chosen, the illusion of control was eliminated when participants were asked to pay to gain more control, and the bias did not affect investment behavior (i.e., participants invested in a risky option the same fraction when faced with more or less control). In the ambiguity treatment, people were willing to pay for less ambiguity, but again the level of ambiguity did not influence investment. Finally, in the myopic loss aversion treatment participants were willing to pay money to have more freedom to choose, even though (in line with the documented bias) they invested less when having more freedom to change their investment.
Differential Mating Success of Male Wing Morphs of the Cricket, Gryllus rubens

-Genetically marked individuals were used to study differential mating success between male wing morphs of the cricket, Gryllus rubens. Previous studies of Gryllus rubens and other wing-dimorphic insects have documented that flightless short-winged or wingless females typically attain reproductive maturity earlier and oviposit more eggs relative to their long-winged counterparts. This study was done to determine if flightless males also exhibit enhanced reproductive characteristics. Segregation analyses documented the genetic basis of allozymes used to infer paternity in subsequent experiments. Control experiments documented the absence of effects on mating success independent of wing morph due to (1) the genetic stock from which males were taken; (2) male size; or (3) female wing morph. Mating trials involving a long-winged male, a short-winged male and a female of either wing morph documented no significant differences in the number of progeny sired by male wing morphs. This pattern was true for both the first group of offspring and for the entire set of offspring produced during a 20-day period. Thus, in contrast to females, we observed no increase in reproductive output in males resulting from the loss of the flight apparatus. However, substantial variance in mating success was observed between males independent of wing morph. This result was likely due to the existence of a dominance hierarchy and the increased mating success of the dominant male.
Kuder Career Search: Test-Retest Reliability and Consequential Validity

The Kuder Career Search with Person-Match (KCS) has a variety of applications; however, the KCS is best suited to help an individual in generating a list of reasonable occupational possibilities. The KCS includes a radical innovation in interest measurement because individuals are not compared to a group within an occupation but are compared to normative responders with a similar KCS response pattern. The primary purpose of this investigation was to examine the test-retest reliability and consequential validity of the KCS. As a group, the internal consistencies for all KCS activity preferences demonstrated adequate consistency. In general, temporal reliability indices for the KCS activity preferences were quite strong. Consequential validity was incorporated with one outcome measure due to the exploratory nature of this portion of the study. The consequential validity of the KCS demonstrated in this study is promising and needs to be more carefully (i.e., methodologically) assessed in future studies.
Culturally mixed groups on international campuses: an opportunity for inter-cultural learning

One of the major educational goals of the internationalisation of higher education is to prepare students to function in an international and inter-cultural context. Cultural diversity on university campuses creates ideal social forums for inter-cultural learning, yet, one of the most disturbing aspects of the internationalisation of higher education in Australia is the lack of interactions between local and international students from Asian backgrounds. This article examines the factors which students believe are affecting the formation of mixed groups for the completion of academic tasks. It also explores the nature of change in students' perceptions after a successful experience of mixed group work. The focus on both local and international students' appraisals of the situation highlights the two-way, interactive nature of group formation and shows how both parties share some responsibility in the lack of cultural mix.
Primary screening method for predicting onset of eating disturbances in female high school students

The purpose of this study is to find out an better screening protocol to identify female adolescents at risk for eating disturbances.We administered a newly-developed eating disturbance onset prediction test and the Eating Attitude Test-26 (the EAT-26) to 1061 Japanese female high school students at Time 1 (T1). EAT-26 was re-administered about 10 months later, designated as Time 2 (T2). Based on EAT-26 findings, students were classified into four eating behavior groups : onset-free group, recovered group, persistent group, and onset group. The normal groups at T1 (onset-free and onset groups) were extracted in Step 1, and the onset group was predicted from the extracted normal groups in Step 2. Useful items for Step 2 were investigated through the eating disturbance onset prediction test, and four items were selected. As a result, the correct discrimination rate in the onset group was obtained as 73.3%, and the negative predictive value was obtained as 98.3%. These results show that the protocol using the four items is useful in predicting the onset of eating disturbances among female high school students. False negative cases accounted for 1.2% of all students, thus, reduction of the false-negative rate by modification of measurement items may be needed.
Energy intake and expenditure during sedentary screen time and motion-controlled video gaming.

BACKGROUND Television watching and playing of video games (VGs) are associated with higher energy intakes. Motion-controlled video games (MC) may be a healthier alternative to sedentary screen-based activities because of higher energy expenditures, but little is known about the effects of these games on energy intakes. OBJECTIVE Energy intake, expenditure, and surplus (intake - expenditure) were compared during sedentary (television and VG) and active (MC) screen-time use. DESIGN Young adults (n = 120; 60 women) were randomly assigned to the following 3 groups: television watching, playing traditional VGs, or playing MCs for 1 h while snacks and beverages were provided. Energy intakes, energy expenditures, and appetites were measured. RESULTS Intakes across these 3 groups showed a trend toward a significant difference (P = 0.065). The energy expenditure (P < 0.001) was higher, and the energy surplus (P = 0.038) was lower, in MC than in television or VG groups. All conditions produced a mean (±SD) energy surplus as follows: 638 ± 408 kcal in television, 655 ± 533 kcal in VG, and 376 ± 487 kcal in MC groups. The OR for consuming ≥500 kcal in the television compared with the MC group was 3.2 (95% CI: 1.2, 8.4). Secondary analyses, in which the 2 sedentary conditions were collapsed, showed an intake that was 178 kcal (95% CI: 8, 349 kcal) lower in the MC condition than in the sedentary groups (television and VG). CONCLUSION MCs may be a healthier alternative to sedentary screen time because of a lower energy surplus, but the playing of these games still resulted in a positive energy balance. This trial was registered at clinicaltrials.gov as NCT01523795.
Diet induced obesity in rats reduces NHE3 and Na+/K+‐ATPase expression in the kidney

The consumption of a high fat diet (HFD) is associated with proteinuria and altered sodium handling and excretion, which can lead to kidney disease. In the proximal tubule, the Na+/H+ Exchanger 3 (NHE3) is responsible for normal protein reabsorption and the reabsorption of approximately 70% of the renal sodium load. It is the Na+/K+‐ATPase that provides the driving force for the reabsorption of sodium and its exit across the basolateral membrane. This study investigates the effects that consumption of a HFD for 12 weeks has on NHE3 and Na+/K+‐ATPase expression in the kidney. Western blot analysis identified a significant reduction in NHE3 and its modulator, phosphorylated protein kinase B, in renal lysate from obese rats. In the obese rats, a reduction in NHE3 expression in the proximal tubule may impact on the acidification of endosomes which are responsible for albumin uptake, suggesting a key role for the exchanger in protein endocytosis in obesity. Western blot analysis identified a reduction in Na+/K+‐ATPase which could also potentially impact on albumin uptake and sodium reabsorption. This study demonstrates that consumption of a HFD for 12 weeks reduces renal NHE3 and Na+/K+‐ATPase expression, an effect that may contribute to the albuminuria associated with obesity. Furthermore the reduction in these transporters is not likely to contribute to the reduced sodium excretion in obesity. These data highlight a potential link between NHE3 and Na+/K+‐ATPase in the pathophysiological changes in renal protein handling observed in obesity.
Heads of Households’ Educational Attainment and Households Food Insecurity and Monetary Poverty in Burkina Faso: A Joint Semi-parametric Bivariate Modeling Approach

Given the Income enabling nature of education as stipulated by human capital theory, it can be postulated that “ceteris paribus”, households with formally educated heads experience less food insecurity and monetary poverty than those with uneducated heads. We test this claim in the case of Burkina Faso, using the 2014 National Survey on Households Living Conditions, along with semi-parametric modeling
Capacidade para o trabalho, saúde e ausência por doença de trabalhadoras de um centro de pesquisa por grupos de idade

This study evaluated the work ability of female Brazilian workers by age group and occupation and analysed women's registered sickness absences during one year. The work ability of the women was assessed using the Work Ability Index, developed by Finnish researchers. The instrument is based on the self-perception of the workers and is composed of seven items. The research had been developed at the Center for Research and Development of a high-technology company. The final response rate of the subjects was 38%. This article includes only the female workers (n=53). The age of the subjects ranged from 35 to 54 years and 79% of them had undergraduate education in the University. The mean work ability was about the same in the younger and older female group. The analysis of the WAI items showed statistically significant difference in relation to the physical demands in the direction of the younger group. In relation to the number of diseases diagnosed by a physician, the sick leave during the past years and the mental resources, the older group had better performance. The promotion of activities should be planned with the aim to reduce the physical workload of the older employees and to prevent the most prevalent diseases of all employees.
A comparative study of the self‐efficacy beliefs of successful men and women in mathematics, science, and technology careers

The purpose of this study was to explore the personal stories of men who selected careers in science, technology, engineering, or mathematics (STEM) to better understand the ways in which their self-efficacy beliefs were created and subsequently influenced their academic and career choices. Analysis of 10 narratives revealed that mastery experience was the primary source of the men's self-efficacy beliefs. These results are compared to those from Zeldin and Pajares' earlier study involving women in STEM careers. For women, social persuasions and vicarious experiences were the primary sources of self-efficacy beliefs. Together, these findings suggest that different sources are predominant in the creation and development of the self-efficacy beliefs of men and women who pursue STEM careers. The self-efficacy beliefs of men in these male-dominated domains are created primarily as a result of the interpretations they make of their ongoing achievements and successes. Women, on the other hand, rely on relational episodes in their lives to create and buttress the confidence that they can succeed in male-dominated domains. Findings were consistent with the theoretical tenets of A. Bandura's social cognitive theory. © 2008 Wiley Periodicals, Inc. J Res Sci Teach 45: 1036–1058, 2008
Mathematical Modeling Identifies Inhibitors of Apoptosis as Mediators of Positive Feedback and Bistability

The intrinsic, or mitochondrial, pathway of caspase activation is essential for apoptosis induction by various stimuli including cytotoxic stress. It depends on the cellular context, whether cytochrome c released from mitochondria induces caspase activation gradually or in an all-or-none fashion, and whether caspase activation irreversibly commits cells to apoptosis. By analyzing a quantitative kinetic model, we show that inhibition of caspase-3 (Casp3) and Casp9 by inhibitors of apoptosis (IAPs) results in an implicit positive feedback, since cleaved Casp3 augments its own activation by sequestering IAPs away from Casp9. We demonstrate that this positive feedback brings about bistability (i.e., all-or-none behaviour), and that it cooperates with Casp3-mediated feedback cleavage of Casp9 to generate irreversibility in caspase activation. Our calculations also unravel how cell-specific protein expression brings about the observed qualitative differences in caspase activation (gradual versus all-or-none and reversible versus irreversible). Finally, known regulators of the pathway are shown to efficiently shift the apoptotic threshold stimulus, suggesting that the bistable caspase cascade computes multiple inputs into an all-or-none caspase output. As cellular inhibitory proteins (e.g., IAPs) frequently inhibit consecutive intermediates in cellular signaling cascades (e.g., Casp3 and Casp9), the feedback mechanism described in this paper is likely to be a widespread principle on how cells achieve ultrasensitivity, bistability, and irreversibility.