Miracle-dz/UniFAR-FTUs huggingface dataset:
Telomere Shortening, Inflammatory Cytokines, and Anti-Cytomegalovirus Antibody Follow Distinct Age-Associated Trajectories in Humans

A number of biological parameters have been cited as hallmarks of immune aging. However, it is not clear whether these multiple biological changes are the result of common underlying aging processes and follow correlated trajectories, or whether the patterns of change for multiple parameters vary across individuals and reflect heterogeneity in the aging process. Here, we have studied parameters of immune system aging through longitudinal analysis of telomere length, inflammatory cytokines, and antibody titer to cytomegalovirus (CMV) in 465 subjects ranging in age from 21 to 88 years at the first visit, with an average of 13 years (7–19 years) follow-up. We observed a highly variable rate of change in telomere length of PBMCs with a relatively slow average rate of telomere shortening (−16 bp/year). Similarly, there were significant increases with age in vivo in three inflammation-related cytokines (interferon gamma, IL-6, and IL-10) and in anti-CMV IgG titer, which varied widely across individuals as well. We further observed positive correlative changes among different inflammatory cytokines. However, we did not find significant correlations among the rate of changes in telomere length, inflammatory cytokines, and anti-CMV IgG titers. Our findings thus reveal that age-related trajectories of telomere attrition, elevated circulating inflammatory cytokines, and anti-CMV IgG are independent and that aging individuals do not show a uniform pattern of change in these variables. Immune aging processes are complex and vary across individuals, and the use of multiple biomarkers is essential to evaluation of biological aging of the immune system.
Use of DNA, RNA, and Chimeric Templates by a Viral RNA-Dependent RNA Polymerase: Evolutionary Implications for the Transition from the RNA to the DNA World

ABSTRACT All polynucleotide polymerases have a similar structure and mechanism of catalysis, consistent with their evolution from one progenitor polymerase. Viral RNA-dependent RNA polymerases (RdRp) are expected to have properties comparable to those from this progenitor and therefore may offer insight into the commonalities of all classes of polymerases. We examined RNA synthesis by the brome mosaic virus RdRp on DNA, RNA, and hybrid templates and found that precise initiation of RNA synthesis can take place from all of these templates. Furthermore, initiation can take place from either internal or penultimate initiation sites. Using a template competition assay, we found that the BMV RdRp interacts with DNA only three- to fourfold less well than it interacts with RNA. Moreover, a DNA molecule with a ribonucleotide at position −11 relative to the initiation nucleotide was able to interact with RdRp at levels comparable to that observed with RNA. These results suggest that relatively few conditions were needed for an ancestral RdRp to replicate DNA genomes.
On‐call stress among Finnish anaesthetists *

We investigated on‐call stress and its consequences among anaesthetists. A questionnaire was sent to all working Finnish anaesthetists (n = 550), with a response rate of 60%. Four categories of on‐call workload and a sum variable of stress symptoms were formed. The anaesthetists had the greatest on‐call workload among Finnish physicians. In our sample, 68% felt stressed during the study. The most important causes of stress were work and combining work with family. The study showed a positive correlation between stress symptoms and on‐call workload (p = 0.009). Moderate burnout was present in 18%vs 45% (p = 0.008) and exhaustion in 32% and 68% (p = 0.015), in the lowest vs highest workload category, respectively. The symptoms were significantly associated with stress, gender, perceived sleep deprivation, suicidal tendencies and sick leave. Being frequently on call correlates with severe stress symptoms and these symptoms are associated with sick leave.
Pet Therapy Research: A Historical Review

From its unpretentious beginnings in pastoral England to the current interest in scientific research and trials of its use, pet therapy is clearly drawing attention to its benefits. Throughout the 40-year history of pet therapy, nursing and nursing research has been at the very heart. The growing body of research in pet therapy reflects nursing's own evolutionary process. This article reviews the history of pet therapy and discusses the growing body of research illustrating the healing power of animal use.
Internet-based teaching: how to encourage university students to adopt advanced Internet-based technologies?

Despite the growing interest in Internet-based teaching and the application of advanced Internet technologies in education, research investigating the use of advanced Internet-based technologies has been very scarce. The objective of this study is to gain a better understanding of factors influencing students' actual usage of sophisticated Internet-based technologies (IBT) in virtual classroom settings. Informed by theories on general human behavior and media choice behavior, we have constructed a model that explains the contribution of various factors to the students' intention to use the technology as well as their actual usage of it. The model has then been tested empirically in a longitudinal study with a survey. Data collected from 60 (student) respondents show that social factors and the students' perceptions regarding potential consequences of the use of IBT significantly affect their intentions to use these technologies. Our results also indicate that facilitating conditions, student habits, and student intentions were significantly related to the students' actual usage of IBT. The implications of the findings for research and practice are discussed.
Quantum vs Classical Proofs and Subset Verification

We study the ability of efficient quantum verifiers to decide properties of exponentially large subsets given either a classical or quantum witness. We develop a general framework that can be used to prove that QCMA machines, with only classical witnesses, cannot verify certain properties of subsets given implicitly via an oracle. We use this framework to prove an oracle separation between QCMA and QMA using an "in-place" permutation oracle, making the first progress on this question since Aaronson and Kuperberg in 2007. We also use the framework to prove a particularly simple standard oracle separation between QCMA and AM.
INFRARED LUMINOSITIES AND DUST PROPERTIES OF z ≈ 2 DUST-OBSCURED GALAXIES

We present SHARC-II 350 μm imaging of twelve 24 μm bright (F24 μm > 0.8 mJy) Dust-Obscured Galaxies (DOGs) and Combined Array for Research in Millimeter-wave Astronomy (CARMA) 1 mm imaging of a subset of two DOGs. These objects are selected from the Boötes field of the NOAO Deep Wide-Field Survey. Detections of four DOGs at 350 μm imply infrared (IR) luminosities which are consistent to within a factor of 2 of expectations based on a warm-dust spectral energy distribution (SED) scaled to the observed 24 μm flux density. The 350 μm upper limits for the 8 non-detected DOGs are consistent with both Mrk 231 and M82 (warm-dust SEDs), but exclude cold dust (Arp 220) SEDs. The two DOGs targeted at 1 mm were not detected in our CARMA observations, placing strong constraints on the dust temperature: Tdust > 35–60 K. Assuming these dust properties apply to the entire sample, we find dust masses of ≈3 × 108 M☉. In comparison to other dusty z ∼ 2 galaxy populations such as submillimeter galaxies (SMGs) and other Spitzer-selected high-redshift sources, this sample of DOGs has higher IR luminosities (2 × 1013 L☉ versus 6 × 1012 L☉ for the other galaxy populations) that are driven by warmer dust temperatures (>35–60 K versus ∼30 K) and lower inferred dust masses (3 × 108 M☉ versus 3 × 109 M☉). Wide-field Herschel and Submillimeter Common-User Bolometer Array-2 surveys should be able to detect hundreds of these power-law-dominated DOGs. We use the existing Hubble Space Telescope and Spitzer/InfraRed Array Camera data to estimate stellar masses of these sources and find that the stellar to gas mass ratio may be higher in our 24 μm bright sample of DOGs than in SMGs and other Spitzer-selected sources. Although much larger sample sizes are needed to provide a definitive conclusion, the data are consistent with an evolutionary trend in which the formation of massive galaxies at z ∼ 2 involves a submillimeter bright, cold-dust, and star-formation-dominated phase followed by a 24 μm bright, warm-dust and AGN-dominated phase.
Evaluation of two flap designs on the mandibular second molar after third molar extractions

Background: The extraction of third molars is associated with some clinical outcomes and periodontal problems. It is imperative to note that the type of incision used in the surgery for the removal of the impacted third molar is critical. The design of the flap influences the healing of the surgically created defect and damage to the distal periodontal area of the adjacent second molar. However, till date, there have been conflicting reports on the influence of different flap designs used for the surgical removal of impacted third molars. Aim: The present study aimed to comparatively evaluate the clinical outcomes and periodontal status of the adjacent second molar, when two different flap designs, namely, the envelope and the modified triangular flap designs were used. Materials and Methods: Sixty female patients with bilateral impacted third molars completed the study with envelope flap on one side and modified triangular flap design on the other side of the mandible for third molar removal. Clinical parameters including pain, dehiscence and swelling were assessed postoperatively and periodontal probing depth (PPD) on the distal aspect of adjacent second molar were assessed both pre- and post-operatively. Results: The results were assessed on 1, 3 and 8 days for pain using visual analog scale. The subjective perception of swelling was evaluated on 3, 7 and 15 days postoperatively in a similar manner. The results of the periodontal parameters were evaluated both preoperatively and 3 months postoperatively, with cautious exploration using a University of North Carolina (UNC)-15 periodontal probe. The statistically significant results for swelling and PPD were noted for the two flap groups using the Chi-square test (P < 0.05). Conclusion: The study revealed that the modified triangular flap had lesser postoperative PPDs and dehiscence. The envelope flap was better when swelling was analyzed. The pain scores, though slightly higher for the modified triangular flap group, were not statistically significant.
Cancer Patients' Attitudes toward Future Research Uses of Stored Human Biological Materials

The policy debate concerning informed consent for future, unspecified research of stored human biological materials (HBM) would benefit from an understanding of the attitudes of individuals who contribute tissue specimens to HBM repositories. Cancer patients who contributed leftover tissue to the Indiana University Cancer Center Tissue Bank under such conditions were recruited for a mail survey study of their attitudes. Our findings suggest that a clear majority of subjects would permit unlimited future research on stored HBMs without re-contact and re-consent, and a significant minority appear to desire ongoing control over future research uses of their tissue. These differences merit further investigation and suggest that a policy of blanket consent for all future, unspecified research would be premature.
Parametric optimisation of exergy destruction in small DI diesel engine fuelled with neem biodiesel using the Taguchi method

ABSTRACT Large amount of emissions from vehicles have led to the degradation of urban air quality and have resulted in serious health issues. Biodiesel, a substitute fuel for diesel engine, is receiving great attention worldwide. This work investigates the merits of using neem-biodiesel and diesel blends for single cylinder small direct injection diesel engine. The energy (the first law) and exergy (the second law) analyses of direct injection diesel engine using neem-biodiesel blends have been presented. Taguchi’s ‘L’ 16’ orthogonal array has been used for the design of experiments. The engine was tested at different engine speeds, load percentages and blend ratios, using neem biodiesel. The results show that the optimum operating conditions for minimum brake specific fuel consumption are achieved when the engine speed is 1900 rev/min, load percentage is 75 and the engine is fuelled with B40.
Mind the fish: zebrafish as a model in cognitive social neuroscience

Understanding how the brain implements social behavior on one hand, and how social processes feedback on the brain to promote fine-tuning of behavioral output according to changes in the social environment is a major challenge in contemporary neuroscience. A critical step to take this challenge successfully is finding the appropriate level of analysis when relating social to biological phenomena. Given the enormous complexity of both the neural networks of the brain and social systems, the use of a cognitive level of analysis (in an information processing perspective) is proposed here as an explanatory interface between brain and behavior. A conceptual framework for a cognitive approach to comparative social neuroscience is proposed, consisting of the following steps to be taken across different species with varying social systems: (1) identification of the functional building blocks of social skills; (2) identification of the cognitive mechanisms underlying the previously identified social skills; and (3) mapping these information processing mechanisms onto the brain. Teleost fish are presented here as a group of choice to develop this approach, given the diversity of social systems present in closely related species that allows for planned phylogenetic comparisons, and the availability of neurogenetic tools that allows the visualization and manipulation of selected neural circuits in model species such as the zebrafish. Finally, the state-of-the art of zebrafish social cognition and of the tools available to map social cognitive abilities to neural circuits in zebrafish are reviewed.
Camelina Seed Supplementation at Two Dietary Fat Levels Change Ruminal Bacterial Community Composition in a Dual-Flow Continuous Culture System

This experiment aimed to determine the effects of camelina seed (CS) supplementation at different dietary fat levels on ruminal bacterial community composition and how it relates to changes in ruminal fermentation in a dual-flow continuous culture system. Diets were randomly assigned to 8 fermenters (1,200–1,250 mL) in a 2 × 2 factorial arrangement of treatments in a replicated 4 × 4 Latin square with four 10-day experimental periods that consisted of 7 days for diet adaptation and 3 days for sample collection. Treatments were: (1) no CS at 5% ether extract (EE, NCS5); (2) no CS at 8% EE (NCS8); (3) 7.7% CS at 5% EE (CS5); and (4) 17.7% CS at 8% EE (CS8). Megalac was used as a control to adjust EE levels. Diets contained 55% orchardgrass hay and 45% concentrate, and fermenters were equally fed a total of 72 g/day (DM basis) twice daily. The bacterial community was determined by sequencing the V4 region of the 16S rRNA gene using the Illumina MiSeq platform. Sequencing data were analyzed using mothur and statistical analyses were performed in R and SAS. The most abundant phyla across treatments were the Bacteroidetes and Firmicutes, accounting for 49 and 39% of the total sequences, respectively. The bacterial community composition in both liquid and solid fractions of the effluent digesta changed with CS supplementation but not by dietary EE. Including CS in the diets decreased the relative abundances of Ruminococcus spp. , Fibrobacter spp. , and Butyrivibrio spp. The most abundant genus across treatments, Prevotella, was reduced by high dietary EE levels, while Megasphaera and Succinivibrio were increased by CS supplementation in the liquid fraction. Correlatively, the concentration of acetate was decreased while propionate increased; C18:0 was decreased and polyunsaturated fatty acids, especially C18:2 n-6 and C18:3 n-3, were increased by CS supplementation. Based on the correlation analysis between genera and fermentation end products, this study revealed that CS supplementation could be energetically beneficial to dairy cows by increasing propionate-producing bacteria and suppressing ruminal bacteria associated with biohydrogenation. However, attention should be given to avoid the effects of CS supplementation on suppressing cellulolytic bacteria.
Localization of the vacuolar-type ATPase in swimbladder gas gland cells of the European eel (Anguilla anguilla)

SUMMARY The vacuolar ATPase is a multifunctional enzyme that consists of several subunits. Subunit B is part of the catalytic domain of the enzyme and is present in two isoforms in fish as well as in mammals. Possibly, these two isoforms — vatB1 (kidney isoform) and vatB2 (brain isoform) — serve different functions. A localization of the two isoforms was attempted in swimbladder gas gland cells of the European eel Anguilla anguilla by immunohistochemistry. Two antibodies were produced by immunization of rabbits with synthetic peptides. Specificity of the antibodies, on the one hand, an isoform-specific antibody for vatB1 and, on the other hand, an antibody that recognizes both isoforms (vatB1 and vatB2), was confirmed by western blot analysis using recombinant proteins produced in a bacterial expression system. The immunohistochemical localization with the antibody directed against both isoforms of the B subunit revealed a positive staining in apical membranes of swimbladder gas gland cells as well as in the basolateral membranes. Significant staining was observed in vesicles located near the apical membrane. Staining with the vatB1-specific antibody resulted in a similar picture in the apical region of the cells. In contrast to the staining with the first antibody, only a poor signal was observed in the basal region. The nature of the vesicles in the apical region of the gas gland cells was determined by using an antibody directed against surfactant protein D.
Adolescent alcohol use is a risk factor for adult alcohol and drug dependence: evidence from a twin design

Background. Early alcohol use is associated with abuse and dependence of licit and illicit substances later in life. The role of genetic and environmental factors in this association is not conclusive. Method. In 1992, data on substance use, abuse/dependence and psychiatric disorders were collected from 8169 male twin members of the Vietnam Era Twin Registry. The interview obtained age of onset of regular drinking (one drink/month for 6 or more months). Regression analyses of twin pairs discordant for early alcohol use tested whether the association between early drinking (before age 17) and adult substance use and abuse/dependence remained after controlling for genetic factors, family environment and covariates. Twin models tested for common genetic and/or environmental influences on early drinking and adult alcohol dependence and ever use and abuse/dependence on marijuana and other drugs. Results. Co-twin analyses suggested the association between early regular alcohol use and adult alcohol dependence, marijuana and other drug use, and marijuana and other drug abuse/dependence could not be entirely explained by common genetic and shared family environmental factors. Genetic contributions to early regular drinking were significantly correlated with those on use of marijuana (rA=0·59), use of other drugs (rA=0·64), alcohol dependence (rA=0·54) and abuse/dependence of marijuana and other drugs (rA=0·63 and 0·66). Small but significant unique environmental correlations (rE range 0·11–0·22) indicated that familial factors could not entirely explain the association between early alcohol use and later substance use, abuse and dependence. Conclusions. Early regular drinking is associated with later alcohol dependence and use, abuse/dependence on drugs. The association is not entirely explained by genetic or shared family environmental factors. This suggests unique environmental factors contribute to transitions from early regular alcohol drinking to use, abuse and dependence on alcohol and other substances.
Tooth loss, chewing ability and quality of life

Objectives: The aim of this study was to observe the tooth loss over age in a sample of Brazilian patients and analyze their ability to chew, relating it to how much is the loss of oral function impact over the quality of life (QoL). Materials and Methods: This is a single center, observational study and the data were collected through clinical examination followed of questionnaires to obtain sociodemographic information, the ability to chew (through the index of chewing ability [ICA]) and QoL (through Oral Health Impact Profile, OHIP-14). Results: The sample was composed by 171 random volunteers with mean age of 47 (SD 15.2). Low number of natural teeth was associated with an increase of age (Spearman's rho correlation coefficient-0.7, P < 0.001, 2-tailed) and chew disability (ICA: chew's ability vs. disability) (Mann-Whitney U-Test, P < 0.001). Chew disability showed a negative impact over the QoL (overall OHIP; Mann-Whitney U Test P < 0.001) and in five of seven OHIP domains (Functional Limitation, Physical Pain, Psychological Discomfort, Physical Disability, Psychological Disability). Age over than 40 years, was also associated with chewing disability (Pearson Chi-Square P < 0.001) and poorer QoL (Mann-Whitney U test P = 0.01). Conclusion: This study observed that the chewing disability produces a significant and negative impact over oral-health related QoL and both, poor QoL and chewing disability are related with the decrease of the number of natural teeth.
Arcaid: Addressing Situation Awareness and Simulator Sickness in a Virtual Reality Pac-Man Game

This paper describes the challenges of converting the classic Pac-Man arcade game into a virtual reality game. Arcaid provides players with the tools to maintain sufficient situation awareness in an environment where, unlike the classic game, they do not have full view of the game state. We also illustrate methods that can be used to reduce a player's simulation sickness by providing visual focal points for players and designing user interface elements that do not disrupt immersion.
Heart Disease Prediction System using Naive Bayes

` Abstract: As large amount of data is generated in medical organisations (hospitals,medical centers) but as this data is not properly used. There is a wealth of hidden information present in the datasets. This unused data can be converted into useful data. For this purpose we can use different data mining techniques. This paper presents a classifier approach for detection of heart disease and shows how Naive Bayes can be used for classification purpose. In our system, we will categories medical data into five categories namely no,low, average,high and very high.Also, if unknown sample comes then the system will predict the class label of that sample. Hence two basic functions namely classification (training) and prediction (testing) will be performed. Accuracy of the system is depends on algorithm and database used.