Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel analysis model for implant osseointegration using ectopic bone formation via the recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system in rats: a proof-of-concept study

Purpose The osseointegration around titanium mini-implants installed in macroporous biphasic calcium phosphate (MBCP) blocks was evaluated after incubation with recombinant human bone morphogenetic protein-2 (rhBMP-2) in an ectopic subcutaneous rat model. Methods Mini-implants (φ1.8×12 mm) were installed in MBCP blocks (bMBCPs, 4×5×15 mm) loaded with rhBMP-2 at 0.1 mg/mL, and then implanted for 8 weeks into subcutaneous pockets of male Sprague-Dawley rats (n=10). A histomorphometric analysis was performed, and the bone-to-implant contact (BIC) and bone density were evaluated. Results Significant osteoinductive activity was induced in the rhBMP-2/bMBCP group. The percentage of BIC was 41.23±4.13% (mean±standard deviation), while bone density was 33.47±5.73%. In contrast, no bone formation was observed in the bMBCP only group. Conclusions This model represents a more standardized tool for analyzing osseointegration and bone healing along the implant surface and in bMBCPs that excludes various healing factors derived from selected animals and defect models.
Low specificity and sensitivity of smell identification testing for the diagnosis of Parkinson's disease.

OBJECTIVE The aim of this study is to determine if the University of Pennsylvania's Smell Identification Test (UPSIT) is an accurate diagnostic tool for olfactory dysfunction in Parkinson's disease (PD). METHOD We included 138 non-demented PD subjects and 175 control subjects matched by gender. Smell identification was tested using UPSIT. RESULTS The mean number of UPSIT items correctly identified by controls was 27.52 ± 5.88; the mean score for PD subjects was 19.66 ± 6.08 (p=<0.001). UPSIT sensitivity was 79.7% with a specificity of 68.5% using a cut-off score of ≤ 25. The overall accuracy for the diagnosis of PD was of 75.3%. CONCLUSION UPSIT accuracy and specificity were lower than what has been previously reported. Our data demonstrates that 17.5% of items of the UPSIT were not well identified by healthy controls. Further research of the identification of a truly cross-cultural test is warranted.
On the enthalpy of melting of poly(l-lactide)

Abstract Poly(l-lactide) (PLLA) is an extensively employed biodegradable semicrystalline polymer with usage from implantable medical devices and drug release matrices to environmentally friendly packaging materials. Since the morphology and crystallinity considerably determine the application field, PLLA samples with a large variation in crystallinity (going from below 20% to over 70%) were prepared by different procedures. Subsequent differences in morphology, crystallinity and thermal properties were followed by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD) measurements and differential scanning calorimetry (DSC). The value of the enthalpy of melting of a perfectly (100%) crystalline PLLA was determined (ΔHf = 115.7 J/g).
Distraction therapy with nature sights and sounds reduces pain during flexible bronchoscopy: a complementary approach to routine analgesia.

STUDY OBJECTIVES To determine whether distraction therapy with nature sights and sounds during flexible bronchoscopy (FB) reduces pain and anxiety. DESIGN Randomized controlled trial. SETTING Teaching hospital in Baltimore, MD. PATIENTS Consecutive adult patients (n = 80) undergoing FB with conscious sedation. INTERVENTION Nature scene murals were placed at the bedside, and patients were provided a tape of nature sounds to listen to before, during, and after the procedure. Patients assigned to the control group were not offered either the nature scene or the sounds. MEASUREMENTS AND RESULTS The primary outcomes were patient ratings of pain control (a 5-point scale ranging from poor to excellent) and anxiety. In a multivariate ordinal logistic regression model, the odds of better pain control were greater in the intervention patients than in the control patients (odds ratio [OR], 4.76; 95% confidence interval [CI], 1.35 to 16.7), after adjustment for age, gender, race, education, health status, and dose of narcotic medication. Older patients and patients with better health status reported significantly less pain. There was no difference in patient-reported anxiety between the two groups (OR, 0.87; 95% CI, 0.39 to 1.96). CONCLUSIONS Distraction therapy with nature sights and sounds significantly reduces pain in patients undergoing FB. Although the precise mechanism of this beneficial effect requires further investigation, clinicians should consider this nonintrusive strategy in addition to standard analgesic medications in patients undergoing painful, invasive procedures.
An intervention designed to improve sensory impairments in the elderly and indoor lighting in their homes: an exploratory randomized controlled trial

Background Vision and hearing impairments in the elderly (aged over 80 years) and poor indoor lighting conditions in a home-care setting are risk factors for functional decline, reduced social participation, withdrawal, and accidents. Objective We aimed to evaluate the changes in vision, hearing, and lighting conditions in the homes of participants aged over 80 years after implementation of a clinical intervention. Methods We undertook an exploratory randomized, controlled experimental study of sensory impairments and lighting conditions in the homes of elderly aged over 80 years who received home care. The intervention group (IG) received advice and encouragement to improve their vision, hearing, and indoor lighting conditions in the home, with a 10-week follow-up period. The control group (CG) received their usual care and underwent the same vision and hearing tests but were provided no intervention. Results Vision and hearing (self-assessed) and tested by Wilcoxon rank-sum test were significantly better (P=0.025 and P=0.008, respectively) in the IG after the intervention and follow-up. The test between the groups showed a significance of P=0.026 for visual acuity and P=0.098 for pure-tone average. The maximum and minimum lighting levels were significantly improved in the IG after the intervention (P=0.002 and P=0.039, respectively) but were unchanged in the CG. Conclusion Several of the IG participants did not follow all of the advice; however, among those who did, vision, hearing, and lighting conditions were all significantly improved. It appears that modest interventions have great potential for improving vision and hearing. Older patients in the home-care setting cannot be expected to take the necessary action to improve their sensory impairments by themselves. They require close monitoring, help from a specialist, and help to improve the indoor lighting conditions in their homes.
Locally decodable codes with 2 queries and polynomial identity testing for depth 3 circuits

In this work we study two, seemingly unrelated, notions. Locally Decodable Codes (LDCs) are codes that allow the recovery of each message bit from a constant number of entries of the codeword. Polynomial Identity Testing (PIT) is one of the fundamental problems of algebraic complexity: we are given a circuit computing a multivariate polynomial and we have to determine whether the polynomial is identically zero. We improve known results on locally decodable codes and on polynomial identity testing and show a relation between the two notions. In particular we obtain the following results:We show that if E: Fn → Fm is a linear LDC with 2 queries then m = exp(Ω(n)). Previously this was only known for fields of size << 2n [18]. We show that from every depth 3 arithmetic circuit (ΣΠΣ circuit), C, with a bounded (constant) top fan-in that computes the zero polynomial, one can construct a locally decodeable code. More formally: Assume that C is minimal (no subset of the multiplication gates sums to zero) and simple (no linear function appears in all the multiplication gates). Denote by d the degree of the polynomial computed by C and by r the rank of the linear functions appearing in C. Then we can construct a linear LDC with 2 queries, that encodes messages of length r/polylog(d) by codewords of length O(d). We prove a structural theorem for ΣΠΣ circuits, with a bounded top fan-in, that compute the zero polynomial. In particular we show that if such a circuit is simple and minimal and of polynomial size then its rank, r, is only polylogarithmic in the number of variables (a priory it could have been linear). We give new PIT algorithms for ΣΠΣ circuits with a bounded top fan-in:A deterministic algorithm that runs in quasi polynomial time.A randomized algorithm that runs in polynomial time and uses only polylogarithmic number of random bits.Moreover, when the circuit is multilinear our deterministic algorithm runs in polynomial time. Previously, deterministic subexponential time algorithms for PIT in bounded depth circuits were known only for depth 2 circuits (in the black box model)[22, 9, 28]. In particular, for the special case of depth 3 circuits with 3 multiplication gates our result resolves an open question asked by Klivans and Spielman [28].
Insulin-like Growth Factor-I and Risk of High-Grade Cervical Intraepithelial Neoplasia

Insulin-like growth factors (IGF) and their binding proteins (IGFBP) have been implicated in the risk of several epithelial or glandular tumors, including prostate cancer, breast cancer, and colon cancer. Cervical cancer, which is also of epithelial origin, has been shown to overexpress receptors for IGF-I, and plasma levels of IGF-I have been positively associated with cervical cancer precursors in one epidemiologic study. In this case-control study, we investigated plasma levels of IGF-I and IGFBP-3 in relation to the risk of histologically confirmed high-grade cervical intraepithelial neoplasia (HGCIN) and the risk of human papillomavirus (HPV) infection. Included in this analysis were 329 cases and 621 controls recruited from clinics affiliated with two Montréal-area hospital centers. We observed a reduced risk of HGCIN for increasing levels of IGF-I, with an adjusted odds ratio (OR) of 0.40 (95% confidence interval, 0.19-0.87) for the highest quartile relative to the lowest quartile of IGF-I. No association was observed between IGFBP-3 levels and HGCIN. Among controls, IGF-I was associated with a decreased risk of being positive for HPV-16 or HPV-18, with an adjusted odds ratio of 0.20 (95% confidence interval, 0.05-0.87) for the highest quartile relative to the lowest quartile of IGF-I. There was no association observed between IGFBP-3 levels and HPV infection status. IGF-I–mediated effects seemed to predominate among women <30 years of age. In contrast to the previously reported study, our results suggest that levels of IGF-I in young women may be inversely associated with HGCIN, a precursor to cervical cancer. (Cancer Epidemiol Biomarkers Prev 2007;16(4):716–22)
Modeling the Principles of Community-Based Participatory Research in a Community Health Assessment Conducted by a Health Foundation

The authors discuss strategies used and lessons learned by a health foundation during development of a community health assessment model incorporating community-based participatory research (CBPR) approaches. The assessment model comprises three models incorporating increasing amounts of CPBR principles. Model A combines local-area analysis of quantitative data, qualitative information (key informants, focus groups), and asset mapping. Model B, a community-based participatory model, emphasizes participatory rural appraisal approaches and quantitative assessment using rapid epidemiological assessment. Model C, a modified version of Model B, is financially more sustainable for our needs than Model B. The authors (a) describe origins of these models and illustrate practical applications and (b) explore the lessons learned in their transition from a traditional, nonparticipatory, quantitative approach to participatory approaches to community-health assessment. It is hoped that this article will contribute to the growing body of knowledge of practical aspects of incorporating CBPR approaches into community health assessments.
Functional characteristics of lactobacilli from traditional Bulgarian fermented milk products

After oral administration, probiotic lactobacilli meet a number of protection systems in the human body, such as exposure to gastric, pancreatic, and small intestinal juices. Overcoming these detrimental barriers allows living bacteria to adhere to the intestinal epithelium and permanently colonize the gastrointestinal tract (GIT), providing health benefits to the host. Based on this, the transit tolerance of 25 candidate probiotic lactobacilli from katak, yoghurt, and white-brined and yellow cheese to simulated bile and small intestinal juices of variable pH was investigated. To establish their resistance, in vitro model systems based on modified MRS media and a longer duration of action (up to 24 h of incubation) were designed. Six of the strains studied were found to show strain-specific survival capacity with low viability in conditions simulating stomach acidity and high resistance to bile and intestinal juices. In addition, the adherence capability (autoaggregation and hydrophobicity) of the strains was determined. Obtained results allowed to select Lactobacillus strains with high survival ratios while passing through the GIT and good adherence properties, which make them suitable for the development of new probiotics.
Heterologous Prime-Boost Regimens with a Recombinant Chimpanzee Adenoviral Vector and Adjuvanted F4 Protein Elicit Polyfunctional HIV-1-Specific T-Cell Responses in Macaques

HIV-1-specific CD4+ and CD8+ T lymphocytes are important for HIV-1 replication control. F4/AS01 consists of F4 recombinant fusion protein (containing clade B Gag/p24, Pol/RT, Nef and Gag/p17) formulated in AS01 Adjuvant System, and was shown to induce F4-specific polyfunctional CD4+ T-cell responses in humans. While replication-incompetent recombinant HIV-1/SIV antigen-expressing human adenoviral vectors can elicit high-frequency antigen-specific CD8+ T-cell responses, their use is hampered by widespread pre-existing immunity to human serotypes. Non-human adenovirus serotypes associated with lower prevalence may offer an alternative strategy. We evaluated the immunogenicity of AdC7-GRN (‘A’), a recombinant chimpanzee adenovirus type 7 vector expressing clade B Gag, RT and Nef, and F4/AS01 (‘P’), when delivered intramuscularly in homologous (PP or AA) and heterologous (AAPP or PPAA) prime-boost regimens, in macaques and mice. Vaccine-induced HIV-1-antigen-specific T cells in peripheral blood (macaques), liver, spleen, and intestinal and genital mucosa (mice) were characterized by intracellular cytokine staining. Vaccine-specific IgG antibodies (macaques) were detected using ELISA. In macaques, only the heterologous prime-boost regimens induced polyfunctional, persistent and balanced CD4+ and CD8+ T-cell responses specific to each HIV-1 vaccine antigen. AdC7-GRN priming increased the polyfunctionality of F4/AS01-induced CD4+ T cells. Approximately 50% of AdC7-GRN-induced memory CD8+ T cells exhibited an effector-memory phenotype. HIV-1-specific antibodies were detected with each regimen. In mice, antigen-specific CD4+ and CD8+ T-cell responses were detected in the mucosal and systemic anatomical compartments assessed. When administered in heterologous prime-boost regimens, AdC7-GRN and F4/AS01 candidate vaccines acted complementarily in inducing potent and persistent peripheral blood HIV-1-specific CD4+ and CD8+ T-cell responses and antibodies in macaques. Besides, adenoviral vector priming modulated the cytokine-expression profile of the protein-induced CD4+ T cells. Each regimen induced HIV-1-specific T-cell responses in systemic/local tissues in mice. This suggests that prime-boost regimens combining adjuvanted protein and low-seroprevalent chimpanzee adenoviral vectors represent an attractive vaccination strategy for clinical evaluation.
Germanium Crystal Chemistry in Cu-Bearing Sulfides from Micro-XRF Mapping and Micro-XANES Spectroscopy

Germanium is considered a critical element, with a demand that has sharply increased due to booming high-technology industries. To understand Ge incorporation mechanisms in natural systems, we investigate Ge speciation in Cu-bearing sulfide minerals using synchrotron X-ray fluorescence (XRF) chemical mapping and Ge K-edge µ-X-ray absorption near-edge structures (µ-XANES) spectroscopy. The samples investigated include (i) a homogeneous chalcopyrite from the Kipushi polymetallic deposit (Central African copperbelt, D.R. Congo) and (ii) a zoned Ge-rich chalcopyrite from the Barrigão Cu deposit (Iberian pyrite belt, Portugal). First, our spectroscopic analysis supports the occurrence of tetrahedrally-coordinated Ge4+ in chalcopyrite, independently from origins or zoning patterns observed for these minerals. Then, based on statistical analyses of XRF chemical maps, we demonstrate that tetravalent germanium most likely incorporates chalcopyrite through the Fe crystallographic site via coupled substitutions with the following form: (2x + 3y)Fe3+ ⟷ (x + 2y)(Ge,Sn)4+ + x(Zn,Pb)2+ + y(Cu,Ag)+, although the presence of lattice vacancies cannot be completely excluded.
Comparing Visual Assembly Aids for Augmented Reality Work Instructions

Increased product complexity and the focus on zero defects, especially when manufacturing complex engineered products, means new tools are required for helping workers conduct challenging assembly tasks. Augmented reality (AR) has shown considerable promise in delivering work instructions over traditional methods. Many proof-of-concept systems have demonstrated the feasibility of AR but little work has been devoted to understanding how users perceive different AR work instruction interface elements. This paper presents a between-subjects study looking at how interface elements for object depth placement in a scene impact a user’s ability to quickly and accurately assemble a mock aircraft wing in a standard work cell. For object depth placement, modes with varying degrees of 3D modeled occlusion were tested, including a control group with no occlusion, virtual occlusion, and occlusion by contours. Results for total assembly time and total errors indicated no statistically significant difference between interfaces, leading the authors to conclude a floor has been reached for optimizing the current assembly when using AR for work instruction delivery. However, looking at a handful of highly error prone steps showed the impact different types of occlusion have on helping users correctly complete an assembly task. The results of the study provide insight into how to construct an interface for delivering AR work instructions using occlusion. Based on these results, the authors recommend customizing the occlusion method based on the features of the required assembly task. The authors also identified a floor effect for the steps of the assembly process, which involved picking the necessary parts from tables and bins. The authors recommend using vibrant outlines and large textual cues (e.g., numbers on parts bins) as interface elements to guide users during these types of “picking” steps.
Adrenomedullin Enhances Invasion by Trophoblast Cell Lines1

Abstract We have tested the hypothesis that adrenomedullin (ADM), a multifunctional peptide hormone, works as a trophoblast proinvasion factor. Our results showed that ADM receptor components—the mRNA and proteins of calcitonin receptor-like receptor (CALCRL) and receptor activity modifying proteins (RAMPs)—were expressed by human choriocarcinoma JAr cells and first-trimester cytotrophoblast HTR-8/SV neo cells. ADM stimulates both JAr and HTR-8/SV neo cell proliferation. The invasion capabilities of JAr cells and HTR-8/SV neo cells were also enhanced by ADM, and this was associated with increased gelatinolytic activity and reduced plasminogen activator inhibitor-1 mRNA expression (SERPINE1). Our data support the notion that ADM may be involved in the human implantation process via regulating trophoblast proliferation and differentiation.
Structural MRI markers of brain aging early after ischemic stroke

Objective: To examine associations between ischemic stroke, vascular risk factors, and MRI markers of brain aging. Methods: Eighty-one patients (mean age 67.5 ± 13.1 years, 31 left-sided, 61 men) with confirmed first-ever (n = 66) or recurrent (n = 15) ischemic stroke underwent 3T MRI scanning within 6 weeks of symptom onset (mean 26 ± 9 days). Age-matched controls (n = 40) completed identical testing. Multivariate regression analyses examined associations between group membership and MRI markers of brain aging (cortical thickness, total brain volume, white matter hyperintensity [WMH] volume, hippocampal volume), normalized against intracranial volume, and the effects of vascular risk factors on these relationships. Results: First-ever stroke was associated with smaller hippocampal volume (p = 0.025) and greater WMH volume (p = 0.004) relative to controls. Recurrent stroke was in turn associated with smaller hippocampal volume relative to both first-ever stroke (p = 0.017) and controls (p = 0.001). These associations remained significant after adjustment for age, sex, education, and, in stroke patients, infarct volume. Total brain volume was not significantly smaller in first-ever stroke patients than in controls (p = 0.056), but the association became significant after further adjustment for atrial fibrillation (p = 0.036). Cortical thickness and brain volumes did not differ as a function of stroke type, infarct volume, or etiology. Conclusions: Brain structure is likely to be compromised before ischemic stroke by vascular risk factors. Smaller hippocampal and total brain volumes and increased WMH load represent proxies for underlying vascular brain injury.
Multilevel object-oriented classification of quickbird images for urban population estimates

This paper is committed to explore object-oriented methods for the classification of Quickbird images, aiming to support future urban population estimates. The study area concerns the southern sector of São José dos Campos city, located in the State of São Paulo, Brazil. By means of a multi-resolution segmentation approach and a six-layer hierarchical classification network, homogeneous residential areas were identified in terms of density of occupation and building standards (single dwelling units or high-rise buildings). The classification network was built upon spectral, geometrical and topological features of the objects in each level of segmentation as well as upon their contextual and semantic interrelationships in-between the hierarchical levels. The final classification of homogeneous residential units was subject to validation, using an object-based Kappa statistics.
The role of CTLA-4 in tolerance induction and T cell differentiation in experimental autoimmune encephalomyelitis: i.p. antigen administration.

Recent evidence suggests that co-stimulation provided by B7 molecules through CTLA-4 is important in establishing peripheral tolerance. In the present study, we examined the kinetics of tolerance induction and T cell differentiation following i.p. administration of myelin basic protein (MBP) Ac1-11 in mice transgenic for a TCR V(beta)8.2 gene derived from an encephalitogenic T cell clone specific for MBP Ac1-11. Examination of the lymph node cell response after antigen administration demonstrated a dependence on CTLA-4 for i.p. tolerance induction. Examination of splenocyte responses suggested that i.p. antigen administration induced a T(h)2 response, which was potentiated by anti-CTLA-4 administration. Interestingly, i.p. tolerance was able to inhibit the induction of experimental autoimmune encephalomyelitis and anti-CTLA-4 administration did not alter this phenotype, suggesting that CTLA-4 blockade did not block tolerance induction. Thus, T cell differentiation and the dependence on CTLA-4 for tolerance induction following i.p. antigen administration differs between lymph node and spleen in a model of organ-specific autoimmunity.