Miracle-dz/UniFAR-FTUs huggingface dataset:
SEROSURVEY OF VIRAL INFECTIONS IN FREE-RANGING NAMIBIAN CHEETAHS (ACINONYX JUBATUS)

Cheetahs (Acinonyx jubatus) in captivity have unusually high morbidity and mortality from infectious diseases, a trait that could be an outcome of population homogeneity or the immunomodulating effects of chronic stress. Free-ranging Namibian cheetahs share ancestry with captive cheetahs, but their susceptibility to infectious diseases has not been investigated. The largest remaining population of free-ranging cheetahs resides on Namibian farmlands, where they share habitat with domestic dogs and cats known to carry viruses that affect cheetah health. To assess the extent to which free-ranging cheetahs are exposed to feline and canine viruses, sera from 81 free-ranging cheetahs sampled between 1992 and 1998 were evaluated for antibodies against canine distemper virus (CDV), feline coronavirus (feline infectious peritonitis virus; FCoV/FIPV), feline herpesvirus 1 (FHV1), feline panleukopenia virus (FPV), feline immunodeficiency virus (FIV), and feline calicivirus (FCV) and for feline leukemia virus (FeLV) antigens. Antibodies against CDV, FCoV/FIPV, FHV1, FPV, and FCV were detected in 24, 29, 12, 48, and 65% of the free-ranging population, respectively, although no evidence of viral disease was present in any animal at the time of sample collection. Neither FIV antibodies nor FeLV antigens were present in any free-ranging cheetah tested. Temporal variation in FCoV/FIPV seroprevalence during the study period suggested that this virus is not endemic in the free-ranging population. Antibodies against CDV were detected in cheetahs of all ages sampled between 1995 and 1998, suggesting the occurrence of an epidemic in Namibia during the time when CDV swept through other parts of sub-Saharan Africa. This evidence in free-ranging Namibian cheetahs of exposure to viruses that cause severe disease in captive cheetahs should direct future guidelines for translocations, including quarantine of seropositive cheetahs and preventing contact between cheetahs and domestic pets.
A combined wind wave–tidal model for the Venice lagoon, Italy

[1] A numerical model that combines wind waves with tidal fluxes in a tidal basin is presented and validated. The model couples a hydrodynamic finite element module based on the shallow water equations with a finite volume module that accounts for the generation and propagation of wind waves. The wave module solves the wave action conservation on the same triangular mesh used in the hydrodynamic module, thus efficiently reproducing the physical relationships between waves and tide propagation. The combined wind wave–tidal model is applied to the Venice lagoon, Italy. The highly irregular bathymetry of this tidal environment, characterized by deep channels, emergent salt marshes, and extensive tidal flats, suggests the introduction of specific hypotheses that simplify the governing equations with a noteworthy increase in efficiency and robustness of the algorithm. Particular attention is devoted to the dissipation of wave energy at the steep boundaries between channels, tidal flats, and salt marshes. Simulations of wave fields generated under specific wind conditions are presented and discussed. The model results are compared, with good agreement, to field data collected in different stations inside the lagoon of Venice. Finally, evidence of the complementary effect of tidal currents and wind waves on bottom shear stresses is presented using the results of different simulations.
Acute heat tolerance of cardiac excitation in the brown trout (Salmo trutta fario)

The upper thermal tolerance and mechanisms of heat-induced cardiac failure in the brown trout (Salmo trutta fario) was examined. The point above which ion channel function and sinoatrial contractility in vitro, and electrocardiogram (ECG) in vivo, started to fail (break point temperature, BPT) was determined by acute temperature increases. In general, electrical excitation of the heart was most sensitive to heat in the intact animal (electrocardiogram, ECG) and least sensitive in isolated cardiac myocytes (ion currents). BPTs of Ca2+ and K+ currents of cardiac myocytes were much higher (>28°C) than BPT of in vivo heart rate (23.5±0.6°C) (P<0.05). A striking exception among sarcolemmal ion conductances was the Na+ current (INa), which was the most heat-sensitive molecular function, with a BPT of 20.9±0.5°C. The low heat tolerance of INa was reflected as a low BPT for the rate of action potential upstroke in vitro (21.7±1.2°C) and the velocity of impulse transmission in vivo (21.9±2.2°C). These findings from different levels of biological organization strongly suggest that heat-dependent deterioration of Na+ channel function disturbs normal spread of electrical excitation over the heart, leading to progressive variability of cardiac rhythmicity (missed beats, bursts of fast beating), reduction of heart rate and finally cessation of the normal heartbeat. Among the cardiac ion currents INa is ‘the weakest link’ and possibly a limiting factor for upper thermal tolerance of electrical excitation in the brown trout heart. Heat sensitivity of INa may result from functional requirements for very high flux rates and fast gating kinetics of the Na+ channels, i.e. a trade-off between high catalytic activity and thermal stability.
Gene Expression Profiles Predict Survival and Progression of Pleural Mesothelioma

Purpose: Clinical outcomes for malignant pleural mesothelioma (MPM) patients having surgery are imprecisely predicted by histopathology and intraoperative staging. We hypothesized that gene expression profiles could predict time to progression and survival in surgically cytoreduced pleural mesothelioma of all stages. Experimental Design: Gene expression analyses from 21 MPM patients having cytoreductions and identical postoperative adjuvant therapy were performed using the U95 Affymetrix gene chip. Using both dChip and SAM, neural networks constructed a common 27 gene classifier, which was associated with either the high-risk and low-risk group of patients. Data were validated using real-time PCR and immunohistochemical staining. The 27 gene classifier was also used for validation in a separate set of 17 MPM patients from another institution. Results: The groups predicted by the gene classifier recapitulated the actual time to progression and survival of the test set with 95.2% accuracy using 10-fold cross-validation. Clinical outcomes were independent of histology, and heterogeneity of progression and survival in early stage patients was defined by the classifier. The gene classifier had a 76% accuracy in the separate validation set of MPMs. Conclusions: These data suggest that pretherapy gene expression analysis of mesothelioma biopsies may predict which patients may benefit from a surgical approach.
Dietary Hesperidin Exerts Hypoglycemic and Hypolipidemic Effects in Streptozotocin-Induced Marginal Type 1 Diabetic Rats

Citrus bioflavonoids may offer some protection against the early stage of diabetes mellitus and the development of complications. We investigated the effect of hesperidin on blood glucose levels, hepatic glucose-regulating enzyme activities, serum insulin and adiponectin levels, serum and hepatic lipid levels, and parameters of bone loss in streptozotocin (STZ)-induced marginal type 1 diabetic rats. Weanling male rats were randomly assigned to experimental 3 groups: a control (C) group, a STZ induced marginal type 1 diabetes (S) group, and a diabetes and hesperidin group, and fed their respective diets for 4 weeks. STZ injection increased blood glucose in rats, but the increase was marginal. Serum and hepatic lipids, serum adiponectin and insulin levels were significantly changed by STZ injection. Dietary hesperidin (10 g/kg diet) decreased blood glucose by altering the activity of glucose-regulating enzymes, and normalized the lipids and adiponectin levels, but did not change bone parameters in the marginal type 1 diabetic rats. Hesperidin showed both hypoglycemic and hypolipidemic effects but did not affect bone tissue and bone metabolic makers in STZ-injected marginal diabetic weanling rats without any body weight loss due to STZ injection.
Finite element buckling analysis of laminated composite sandwich panels with transversely flexible core carrying attached elastic strip

In this article, a three-dimensional finite element model is proposed to study the effect of distributed attached mass with thickness and stiffness on the buckling instability of sandwich panels with transversely flexible cores. Unlike the previous works in the literature which have made use of unified displacement theories, the present model uses different types of finite elements to model the core and the face sheets. It utilizes shell elements for the face sheets and three-dimensional solid elements for the core which enables the model to account for the transverse flexibility of the structure. The motions of the face sheets and the core as well as the attached mass are related through defining constraint equations between the nodes of their respective finite elements based on the concept of master and slave nodes which is incorporated into the finite element analysis program ANSYS through a user-defined subroutine. The validated finite element model is then used to study the effects of size, thickness, material property, aspect ratio, and the position of the attached mass on the buckling load of a sandwich panel under different combinations of boundary conditions. The results presented in this study have hitherto not been reported in the literature.
Technical Note No. 12. Children's Well-being in Contexts of Poverty: Approaches to Research, Monitoring and Participation.

Monitoring, protecting and promoting ‘well-being’ are central to realisation of children’s rights. Yet definitions of the concept are both variable and can appear conceptually confused. Competing research paradigms engage with the concept and its measurement, while applications of well-being in policy are equally contested. This paper outlines some of the major debates, as a starting point for reviewing three contrasting approaches to well-being: indicator-based, participatory and longitudinal research. In particular, it focuses on applications of the concept in contexts of child poverty worldwide. We suggest there are some promising signs of integration amongst these approaches, and argue that well-being does have potential as a bridging concept, at the same time highlighting inequalities, acknowledging diversities, and respecting children’s agency. Drawing on the experience of Young Lives, a 15-year, four-country longitudinal study of child poverty, we suggest that methods for studying child well-being in global contexts should be dynamic and sensitive to culture and time, as well as to the trade-offs that children are required to make between themselves and others. We argue that dynamic approaches are especially important in research with children as they address how people change in time. Well-being is understood by Young Lives to be about real people and the social contexts they inhabit. It can act as a lens - similar to culture - which recognises that outcomes of deprivation are influenced by children and their responses to and interpretation of events. Accessing children’s views in the context of their communities is important and can increase the accuracy and credibility of research data.
Egfr Amplification Specific Gene Expression in Phyllodes Tumours of the Breast

Background: Recently, we were able to show that amplifications of the epidermal growth factor receptor (egfr) gene and the overexpression of EGFR were associated with the initiation and progression of phyllodes tumours. Methods: In order to gain further insights into regulation mechanisms associated with egfr amplifications and EGFR expression in phyllodes tumours, we performed global gene expression analysis (Affymetrix A133.2) on a series of 10 phyllodes tumours, of these three with and seven without amplifications of an important regulatory repeat in intron 1 of egfr (CA-SSR I). The results were verified and extended by means of immunohistochemistry using the tissue microarray method on an extensively characterized series of 58 phyllodes tumours with antibodies against caveolin-1, eps15, EGF, TGF-α, pErk, pAkt and mdm2. Results: We were able to show that the presence of egfr CA-SSR I amplifications in phyllodes tumours was associated with 230 differentially expressed genes. Caveolin-1 and eps15, involved in EGFR turnover and signalling, were regulated differentially on the RNA and protein level proportionally to egfr gene dosage. Further immunohistochemical analysis revealed that the expression of caveolin-1 and eps15 were also significantly correlated with the expression of pAkt (p < 0.05), pERK (p < 0.05), mdm2 (p < 0.01) and EGF (p < 0.001 for caveolin-1). Eps15 and pERK were further associated with tumour grade (p < 0.01 and p < 0.001, respectively). Conclusion: Our results show that amplifications within regulatory sequences of egfr are associated with the expression of eps15 and caveolin-1, indicating an increased turnover of EGFR. The interplay between EGFR and caveolin-1, eps15, pAkt, mdm2 and pERK therefore seems to present a major molecular pathway in carcinogenesis and progression of breast phyllodes tumours.
The Calculation of Some Limiting Distributions Arising in Near‐Integrated Models with GLS Detrending

Many unit root test statistics are based on detrended data, with the method of generalized least squares (GLS) detrending being popular in the setting of a near‐integrated model. This article determines the properties of some associated limiting distributions when the GLS detrending is based on a linear time trend. A fundamental result for the moment generating function of two key functionals of the relevant stochastic process is provided and used to compute probability density functions and cumulative distribution functions, as well as means and variances, of the limiting distributions of some statistics of interest. The exact moments and percentiles of some of these distributions are compared with those obtained by simulations, and it is found that, even with a large number of replications and a large sample size, the errors resulting from the simulation methods are not negligible. Some further applications, including a comparison of limiting power functions of different unit root test statistics and the consideration of a more complicated statistic, are also provided.
Improving machining accuracy with robot deformation compensation

Industrial robots offer a cheaper yet more flexible alternative to the CNC machines in the cleaning and pre-machining applications of automotive aluminum castings. But the low stiffness has limited the application of industrial robots to the machining tasks with very low precision requirement. This paper presents a practical method to compensate the robot deformation caused by the machining force. A constant joint stiffness model based feed forward compensation scheme is implemented in the robot controller. The compensation scheme is shown to be able to reduce the position error by more than 60%. Application test in milling a standard aluminum block has demonstrated the effectiveness of the proposed deformation compensation method. The surface error is reduced from 0.5mm to 0.1mm.
How do Humans Determine Reflectance Properties under Unknown Illumination

Under normal viewing conditions, humans find it easy to distinguish between objects made out of different materials such as plastic, metal, or paper. Untextured materials such as these have different surface reflectance properties, including lightness and gloss. With single isolated images and unknown illumination conditions, the task of estimating surface reflectance is highly underconstrained, because many combinations of reflection and illumination are consistent with a given image. In order to work out how humans estimate surface reflectance properties, we asked subjects to match the appearance of isolated spheres taken out of their original contexts. We found that subjects were able to perform the task accurately and reliably without contextual information to specify the illumination. The spheres were rendered under a variety of artificial illuminations, such as a single point light source, and a number of photographically-captured real-world illuminations from both indoor and outdoor scenes. Subjects performed more accurately for stimuli viewed under real-world patterns of illumination than under artificial illuminations, suggesting that subjects use stored assumptions about the regularities of real-world illuminations to solve the ill-posed problem.
CIDE: An Integrated Development Environment for Microservices

Microservices is a flexible architectural style that has many advantages over the alternative monolithic style. These include better performance and scalability. It is particularly suitable, and widely adopted, for cloud-based applications, because in this architecture a software system consisting of a large suite of services of fine granularity, each running its own process and communicating with the others. However, programming such systems is more complex. In this paper we report on CIDE, an integrated software development environment that helps with this. CIDE supports programming in a novel agent-oriented language called CAOPLE and tests their execution in a cluster environment. We present the architecture of CIDE, discuss its design based on the principles of the DevOps software development methodology, and describe facilities that support continuous testing and seamless integration, two other advantages of Microservices.
Validity of self-obtained vaginal specimens for diagnosis of trichomoniasis

A comparison of self- and clinician-collected vaginal specimens for the diagnosis of trichomoniasis was conducted. The sensitivities of culture methods using self- and clinician-collected specimens were 84.6 and 88.5%, respectively. There was no significant difference between the sensitivities of culture methods using self- and clinician-collected vaginal specimens for the diagnosis of trichomoniasis.
Susceptibility of F/A-18 Flight Controllers to the Falling-Leaf Mode: Nonlinear Analysis

The F/A-18 Hornet aircraft with the original flight control law exhibited a nonlinear out-of-control phenomenon knownas the falling-leafmode. This unstablemodewas suppressed bymodifying the original control law. This paper employs the falling-leaf phenomenon as an example to demonstrate the utility of recently developed nonlinear analysis methods to flight control validation tools. The flight control law is usually validated and verified by performing linear robustness analysis at different trim points and running many Monte Carlo simulations. Additional insight can be gained by using nonlinear analyses. This paper compares the two flight control laws using nonlinear region-of-attraction analyses and Monte Carlo simulations. The results of these nonlinear analyses indicate that the revised flight control law has significantly improved nonlinear robustness properties as compared with baseline design.
Numerical Investigation of the Shear Buckling and Post-Buckling of Thin Steel Plates with FRP Strengthening

The behavior of thin steel plates under shear loading is governed by early diagonal buckling and subsequent formation of an inclined tension field. This behavior describes the load carrying mechanism in deep steel plate girder webs and steel plate shear walls. Traditionally, steel stiffeners are used on the thin plate in some applications to mitigate out-of-plane buckling and encourage shear yielding, which is preferable and a more stable and energy-dissipating load carrying mechanism. However, the cost and practical difficulties associated with welding these stiffeners, especially on very thin plates and for in-service rehabilitation purposes, are considered as major drawbacks. In this paper, the behavior of thin steel plates strengthened with FRP strips was numerically investigated. FRP wraps were hypothesized to act as an elastic support for the thin steel plate in the early loading stages and as an auxiliary load transfer path in the inelastic tension field action. Numerical investigations were carried out using the finite element method and were divided into two phases; buckling and postbuckling. Elastic eigenvalue analysis for buckling and full inelastic analysis with geometrical and material nonlinearity for the post-buckling phase were carried out. FRP fracture and damage were incorporated in the models. Optimum angle of FRP fibers was studied both for buckling mitigation and post-buckling behavior enhancement. A number of FRP strengthening configurations together with a range of common FRP materials were also employed in the analyses. It was concluded that bonding FRP patches on the steel plate can effectively encourage behavior enhancements, especially given appropriate configuration and material properties.
Durable Anti-Icing Coatings Based on Self-Sustainable Lubricating Layer

A versatile, convenient, and cost-effective method that can be used for grafting anti-icing materials onto different surfaces is highly desirable. Based on mussel-inspired chemistry, the anti-icing coating with extremely low ice adhesion is enabled by constructing a self-sustainable lubricating layer, achieved via modifying solid substrates with a highly hydrophilic conjugate of poly(acrylic acid)–dopamine. Both unfreezable and freezable water remain liquidlike at subzero conditions and synergistically fulfill the role of lubrication for reducing the ice adhesion. The anti-icing coatings show excellent stability in harsh environments and durability after the cross-linking. More importantly, this coating can be applied to various substrates and is of great promise for practical applications.
Ondansetron Compared With Doxylamine and Pyridoxine for Treatment of Nausea in Pregnancy: A Randomized Controlled Trial

OBJECTIVE: To evaluate whether ondansetron or the combination of doxylamine and pyridoxine was superior for the treatment of nausea and vomiting of pregnancy. METHODS: This was a double-blind, randomized, controlled trial in which women with nausea and vomiting of pregnancy were assigned to 4 mg of ondansetron plus a placebo tablet or 25 mg pyridoxine plus 12.5 mg of doxylamine for 5 days. The primary outcome was an improvement in nausea as reported on a 100-mm visual analog scale (VAS). Secondary outcomes were a reduction in vomiting on the VAS and the proportion of patients reporting sedation or constipation while using either study regimen. RESULTS: Thirty-six women (18 in each group) were randomized to either ondansetron or pyridoxine and doxylamine, of whom 13 (72%) and 17 (94%) completed follow-up, respectively. There were no differences among the groups with regard to demographic characteristics or baseline nausea. Patients randomized to ondansetron were more likely to have an improvement in their baseline nausea as compared with those using pyridoxine and doxylamine over the course of 5 days of treatment (median VAS score decreased 51 mm [interquartile range 37–64] compared with 20 mm [8–51]; P=.019). Furthermore, women using ondansetron reported less vomiting (median VAS decreased 41 [interquartile range 17–57] compared with 17 [−4 to 38]; P=.049). There was no significant difference between the groups regarding sedation or constipation. CONCLUSION: Our investigation showed ondansetron to be superior to the combination of pyridoxine and doxylamine in the treatment of nausea and emesis in pregnancy. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov, www.clinicaltrials. gov, NCT01668069. LEVEL OF EVIDENCE: I