Miracle-dz/UniFAR-FTUs huggingface dataset:
Prediction of Short‐Term Outcome in Newly Admitted Psychogeriatric Patients

ABSTRACT: Thirty‐six consecutive admissions to a geriatric service recently reorganized to function within a community psychiatric framework were evaluated by means of a series of behavioral and psychologic measures. Assessments upon admission and at three, five and eight weeks and five months afterward, revealed that the greatest therapeutic responses and the most discharges occurred within the first few weeks after admission. Patients remaining for longer periods tended to stabilize at a lower level of functioning or to deteriorate. A combination of two simple measures used in evaluation upon admission and relating to physical and mental functioning was found to predict the patient's status at five months (still in the hospital, dead, or discharged) with 75 percent accuracy. Use of the lambda statistic for judging the predictive power of measures is suggested.
Hepatic HCV RNA before and after treatment with interferon alone or combined with ribavirin

The clinical use of measuring hepatic hepatitis C virus (HCV) RNA before and after therapy in patients with chronic hepatitis C has been assessed in a number of small clinical trials. Viral clearance from the liver may be a better marker of long‐term response than eradication of serum HCV RNA. The aim of this study was to evaluate quantitative hepatic HCV‐RNA measurements before and after antiviral therapy. Two thousand eighty‐nine chronic hepatitis C patients were enrolled in 3 published clinical trials evaluating interferon alfa‐2b alone or with ribavirin either as initial therapy or for interferon relapse. Hepatic HCV‐RNA quantitation was performed with a modified reverse‐transcription polymerase chain reaction (RT‐PCR) before and 24 weeks after therapy in 951 and 1,316 patients, respectively. Pretherapy hepatic HCV‐RNA concentrations correlated best with serum HCV‐RNA concentrations (R = .236, P = .0001) and negatively correlated with alanine transaminase (ALT) values (−0.178, P = .0001), duration of infection (−0.09, P = .02), parenchymal injury (−0.135, P = .0001), histologic activity index (HAI) inflammatory score (−0.085, P = .01), Knodell fibrosis score (−0.072, P = .03), and body weight (−0.078, P = .02). In paired liver biopsy specimens (n = 534), change in hepatic HCV RNA correlated with the change in the HAI (R = .346, P = .0001). Of 400 sustained virologic responders (SVR), 393 (98%) had undetectable hepatic HCV RNA, whereas 7 (2%) had detectable hepatic HCV RNA; 5 have been followed and 2 have had reappearance of serum HCV RNA 12 months after therapy. In conclusion, measurement of hepatic HCV RNA before or after therapy reflects changes observed in serum HCV RNA, and correlates inversely with hepatic inflammation and fibrosis, but otherwise has minimal clinical use.
Dysfunctional Regulation of Endothelial Nitric Oxide Synthase (eNOS) Expression in Response to Exercise in Mice Lacking One eNOS Gene

Background—Previous data suggest that 1 endothelial NO synthase (eNOS) gene is sufficient to allow normal expression and function of eNOS under basal conditions. We hypothesized that this might not hold true for conditions known to increase eNOS gene expression, such as exercise. Methods and Results—Male mice heterozygous for a disruption of the eNOS gene (eNOS+/−) and normal C56Bl/6J mice (eNOS+/+), 3 to 4 months of age, underwent exercise training for 3 weeks. Nontrained mice were exposed to the exercise environment (noise and vibration of the treadmill) without exercise for an identical period. In eNOS+/+ mice (n=7), exercise increased aortic eNOS protein expression by 3.4±0.4-fold (P <0.002). This was associated with a greater vascular cGMP accumulation on stimulation with acetylcholine (P <0.05). Furthermore, exercise training increased eNOS mRNA (1.78±0.4-fold) and protein (1.76±0.17-fold) in left ventricular tissue, as determined by competitive reverse transcription-polymerase chain reaction and Western analysis (P <0.05 for both). In striking contrast, exercise had no effect on aortic eNOS expression and cGMP accumulation in eNOS+/− mice (P >0.05). Thus, although eNOS expression appears to be normal in eNOS+/− mice under basal conditions, these mice are unable to increase eNOS expression during exercise. Conclusions—These findings show that regulation of eNOS expression during exercise requires the presence of both alleles of the gene and may have implications for conditions in which polymorphisms of eNOS are present in only 1 allele in humans. These individuals may have a normal vascular reactivity under basal conditions but may be unable to adapt their vascular reactivity in response to exercise training.
Paternal levels of DNA damage in spermatozoa and maternal parity influence offspring mortality in an endangered ungulate

Understanding which factors influence offspring mortality rates is a major challenge since it influences population dynamics and may constrain the chances of recovery among endangered species. Most studies have focused on the effects of maternal and environmental factors, but little is known about paternal factors. Among most polygynous mammals, males only contribute the haploid genome to their offspring, but the possibility that sperm DNA integrity may influence offspring survival has not been explored. We examined several maternal, paternal and individual factors that may influence offspring survival in an endangered species (Gazella cuvieri). Levels of sperm DNA damage had the largest impact upon offspring mortality rates, followed by maternal parity. In addition, there was a significant interaction between these two variables, so that offspring born to primiparous mothers were more likely to die if their father had high levels of sperm DNA damage, but this was not the case among multiparous mothers. Thus, multiparous mothers seem to protect their offspring from the deleterious effects of sperm DNA damage. Since levels of sperm DNA damage seem to be higher among endangered species, more attention should be paid to the impact of this largely ignored factor among the viability of endangered species.
Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster

The regulatory complex of the 26S proteasome is responsible for the selective recognition and binding of multiubiquitinated proteins. It was earlier shown that the subunit S5a/Rpn10/p54 of the regulatory complex is the only cellular protein capable of binding multiubiquitin chains in an in vitro overlay assay. The role of this subunit in substrate selection, however, is a subject of debate, following the observation that its deletion in Saccharomyces cerevisiae is not lethal and instead causes only a mild phenotype. To study the function of this subunit in higher eukaryotes, a mutant Drosophila strain was constructed by deleting the single copy gene encoding subunit S5a/Rpn10/p54. This deletion caused larval-pupal polyphasic lethality, multiple mitotic defects, the accumulation of higher multimers of ubiquitinated proteins and a huge accumulation of defective 26S proteasome particles. Deletion of the subunit S5a/Rpn10/p54 does not destabilise the regulatory complex and does not disturb the assembly of the regulatory complex and the catalytic core. The pupal lethality is a consequence of the depletion of the maternally provided 26S proteasome during the larval stages and a sudden increase in the proteasomal activity demands during the first few hours of pupal development. The huge accumulation of the fully assembled 26S proteasome in the deletion mutant and the lack of free subunits or partially assembled particles indicate that there is a highly coordinated accumulation of all the subunits of the 26S proteasome. This suggests that in higher eukaryotes, as with yeast, a feedback circuit coordinately regulates the expression of the proteasomal genes, and this adjusts the actual proteasome concentration in the cells according to the temporal and/or spatial proteolytic demands.
Amplification of the Six Major Human Herpesviruses from Cerebrospinal Fluid by a Single PCR

ABSTRACT We used a novel type of primer system, a system that uses stair primers, in which the primer sequences are based on consensus sequences in the DNA polymerase gene of herpesvirus to detect herpesviruses by PCR. A single PCR in a single tube detected the six major herpesviruses that infect the central nervous system: herpes simplex virus type 1 (HSV-1), and type 2 (HSV-2), cytomegalovirus (CMV), Epstein-Barr virus (EBV), varicella-zoster virus (VZV), and human herpesvirus 6 (HHV-6). We used the technique to analyze 142 cerebrospinal fluid (CSF) samples that had been stored at −80°C and compared the results with those obtained previously for the same samples by standard, targeted PCR. Four hundred one targeted PCR tests had been run with the 142 samples to detect HSV-1, HSV-2, CMV, and VZV; screening for EBV and HHV-6 was not prescribed when the samples were initially taken. Eighteen CSF samples tested positive by classic targeted PCR. The herpesvirus consensus PCR detected herpesviruses in 37 samples, including 3 samples with coinfections and 17 viral isolates which were not targeted. Two samples identified as infected by the targeted PCR tested negative by the consensus PCR, and eight samples that tested positive by the consensus PCR were negative by the targeted PCR. One hundred three samples scored negative by both the targeted and the consensus PCRs. This preliminary study demonstrates the value of testing for six different herpesviruses simultaneously by a sensitive and straightforward technique rather than screening only for those viruses that are causing infections as suggested by clinical signs.
Long-term structural changes after mTBI and their relation to post-concussion symptoms

Abstract Primary objective: To investigate sustained structural changes in the long-term (>1 year) after mild traumatic brain injury (mTBI) and their relationship to ongoing post-concussion syndrome (PCS). Research design: Morphological and structural connectivity magnetic resonance imaging (MRI) data were acquired from 16 participants with mTBI and nine participants without previous head injury. Main outcomes and results: Participants with mTBI had less prefrontal grey matter and lower fractional anisotropy (FA) in the anterior corona radiata and internal capsule. Furthermore, PCS severity was associated with less parietal lobe grey matter and lower FA in the corpus callosum. Conclusions: There is evidence for both white and grey matter damage in participants with mTBI over 1 year after injury. Furthermore, these structural changes are greater in those that report more PCS symptoms, suggesting a neurophysiological basis for these persistent symptoms.
Anaemia: the neglected female health problem in developing countries.

BACKGROUND Anaemia is arguably the biggest female health problems in developing countries. The world is still to fully fathom the extent and gravity of the problem. This problem aggravates particularly during and after pregnancies due to increased nutritional demands and inadequate iron supply and is therefore more widespread in parous segments of female population. Objective of this study was to determine prevalence of anaemia in apparently healthy parous female population of Abbottabad and investigate underlying causes. METHODS This descriptive, cross-sectional study was conducted from October 2001 to March 2011 in Abbottabad. Women reporting for various surgical procedures were investigated for anaemia by obtaining history through a structured performa, specifically designed to identify the underlying causes. Sixteen thousand women of childbearing age (20-40 years) were included in the study; 14,800 with history of at least one pregnancy and remaining with no previous pregnancy. Haemoglobin (Hb) was measured using haematology analyser Sysmex (KX21). RESULTS Of the screened women, 60% were found anaemic (Hb < 12 g/dl) 10% of which were severely anaemic (Hb < 6 g/dl). Anaemia was related to socio-demographic and obstetric history characteristics. The prevalence of anaemia was significantly lower in women who used iron supplements during their pregnancies. Lactating mothers not making up for the iron deficiencies during pregnancies usually carried their anaemia postpartum and beyond. Lower gaps between pregnancies also contributed significantly to the problem. CONCLUSION Prevalence and severity of anaemia in reproductive age females reporting to surgical units were found to be exceptionally high. Frequency of anaemia in the parous females of relatively affluent city of Abbotabad indicates that anaemia may be on the rise in developing countries. Addressing pregnancy related anaemia and nutritional deficiencies through intake of supplements on regular basis in women before and between pregnancies is essential.
Inter-annotator Agreement on a Multilingual Semantic Annotation Task

Six sites participated in the Interlingual Annotation of Multilingual Text Corpora (IAMTC) project (Dorr et al., 2004; Farwell et al., 2004; Mitamura et al., 2004). Parsed versions of English translations of news articles in Arabic, French, Hindi, Japanese, Korean and Spanish were annotated by up to ten annotators. Their task was to match open-class lexical items (nouns, verbs, adjectives, adverbs) to one or more concepts taken from the Omega ontology (Philpot et al., 2003), and to identify theta roles for verb arguments. The annotated corpus is intended to be a resource for meaning-based approaches to machine translation. Here we discuss inter-annotator agreement for the corpus. The annotation task is characterized by annotators freedom to select multiple concepts or roles per lexical item. As a result, the annotation categories are sets, the number of which is bounded only by the number of distinct annotator-lexical item pairs. We use a reliability metric designed to handle partial agreement between sets. The best results pertain to the part of the ontology derived from WordNet. We examine change over the course of the project, differences among annotators, and differences across parts of speech. Our results suggest a strong learning effect early in the project.
Robust RGB color image watermarking scheme based on DWT-SVD and chaotic system

This paper presents a robust image watermarking scheme for embedding gray scale watermark into RGB color image. The presented method is based on discrete wavelet transform (DWT), singular value decomposition and chaotic system. DWT and SVD are separately applied on three color planes (R, G and B) of original color image and watermark image for embedding and extracting processes. DWT and SVD techniques are used in the proposed watermarking scheme to increase the information hiding capacity and perceptual similarity of watermarked image. At the same time, chaotic system is also used in the proposed watermarking method to ensure the security of the watermarking technique by scrambling the watermark image. In the embedding stage, the gray scale watermark information is separately embedded into three color planes of the original color image. On the other hand, in the extracting stage, the watermark information is separately extracted from three color components and the final watermark is obtained by calculating the average of extracted results from three color components. The results of the proposed method are compared with other existing image watermarking methods. From the results, it can be seen that the proposed method outperforms the other existing methods by giving high values of peak signal to noise ratio (PSNR) and low values of mean square error (MSE). The proposed approach also shows well robustness against different types of attacks than other existing methods by providing high values of normalized correlation (NC).
T lymphocytes in the murine vaginal mucosa are phenotypically distinct from those in the periphery

The results from both clinical studies of women with recurrent vulvovaginal candidiasis and a murine model of experimental vaginitis indicate that systemic cell-mediated immunity may not represent a dominant host defense mechanism against vaginal infections by Candida albicans. Recent experimental evidence indicates the presence of local vaginal immune reactivity against C. albicans. The present study was designed to examine T-lymphocyte subpopulations in the vaginal mucosae of naive CBA/J mice. Vaginal lymphocytes (VL) were isolated by collagenase digestion of whole vaginal tissues. Cell populations were identified by flow cytometry, and the results were compared with those for both lymph node cells (LNC) and peripheral blood lymphocytes (PBL). The results of flow cytometry showed that 45% +/- 10% of lymphocytes in the vaginal mucosa are CD3+ compared with 75% +/- 5% in LNC and 50% +/- 5% in PBL. The majority (85%) of CD3+ VL are CD4+ and express the alpha/beta T-cell receptor (TCR), similar to the results for LNC and PBL. In contrast to LNC and PBL, VL contain a significantly higher percentage (15 to 20%) of gamma/delta TCR+ cells, 80% or more of which appear to express CD4. In addition, while CD4-CD8 cell ratios in LNC and PBL were 3:1 and 6:1, respectively, only 1% of VL expressed CD8, resulting in a CD4-CD8 cell ratio of > 100:1. Finally, while LNC and PBL recognized two epitope-distinct (GK 1.5 and 2B6) anti-CD4 antibodies, VL recognized only 2B6 anti-CD4 antibodies. Further analysis of VL showed that Thy-1 cells, but not CD4 cells, were reduced after intravaginal injection of complement-fixing anti-Thy-1.2 and GK 1.5 anti-CD4 antibodies, respectively. Taken together, these data suggest that T lymphocytes in the vaginal mucosae of mice are phenotypically distinct from those in the periphery and that CD4+ VL have an uncharacteristic or atypical expression of the CD4 receptor.
Feasibility of Outpatient Fully Integrated Closed-Loop Control

OBJECTIVE To evaluate the feasibility of a wearable artificial pancreas system, the Diabetes Assistant (DiAs), which uses a smart phone as a closed-loop control platform. RESEARCH DESIGN AND METHODS Twenty patients with type 1 diabetes were enrolled at the Universities of Padova, Montpellier, and Virginia and at Sansum Diabetes Research Institute. Each trial continued for 42 h. The United States studies were conducted entirely in outpatient setting (e.g., hotel or guest house); studies in Italy and France were hybrid hospital–hotel admissions. A continuous glucose monitoring/pump system (Dexcom Seven Plus/Omnipod) was placed on the subject and was connected to DiAs. The patient operated the system via the DiAs user interface in open-loop mode (first 14 h of study), switching to closed-loop for the remaining 28 h. Study personnel monitored remotely via 3G or WiFi connection to DiAs and were available on site for assistance. RESULTS The total duration of proper system communication functioning was 807.5 h (274 h in open-loop and 533.5 h in closed-loop), which represented 97.7% of the total possible time from admission to discharge. This exceeded the predetermined primary end point of 80% system functionality. CONCLUSIONS This study demonstrated that a contemporary smart phone is capable of running outpatient closed-loop control and introduced a prototype system (DiAs) for further investigation. Following this proof of concept, future steps should include equipping insulin pumps and sensors with wireless capabilities, as well as studies focusing on control efficacy and patient-oriented clinical outcomes.
Camera Calibration for Plate Refractive Imaging System

This paper presents a study of an important imaging system, called the plate refractive imaging system. The system comprises of a perspective camera looking through one or more planar parallel plates to the target imaging environment. Such as the perspective camera captures the object through an infrared filter which is used in the infrared navigator for surgeries. Sometimes, we have to calibrate the camera without removing the plate. Up to now, it does not have a valid algorithm to do so. In this paper, a plate refractive camera calibration model is proposed for the imaging systems that incorporate a refractive plate at the front end of the camera. The proposed model is based on the refractive effect of variations in the viewpoint of such systems. Both synthetic and real data show that our proposed camera model is insensitive to noise and can achieve high accuracy.
Evidence and Effect of Photogenerated Charge Transfer for Enhanced Photocatalysis in WO3/TiO2 Heterojunction Films: A Computational and Experimental Study

Semiconductor heterojunctions are used in a wide range of applications including catalysis, sensors, and solar‐to‐chemical energy conversion devices. These materials can spatially separate photogenerated charge across the heterojunction boundary, inhibiting recombination processes and synergistically enhancing their performance beyond the individual components. In this work, the WO3/TiO2 heterojunction grown by chemical vapor deposition is investigated. This consists of a highly nanostructured WO3 layer of vertically aligned nanorods that is then coated with a conformal layer of TiO2. This heterojunction shows an unusual electron transfer process, where photogenerated electrons move from the WO3 layer into TiO2. State‐of‐the‐art hybrid density functional theory and hard X‐ray photoelectron spectroscopy are used to elucidate the electronic interaction at the WO3/TiO2 interface. Transient absorption spectroscopy shows that recombination is substantially reduced, extending both the lifetime and population of photogenerated charges into timescales relevant to most photocatalytic processes. This increases the photocatalytic efficiency of the material, which is among the highest ever reported for a thin film. In allying computational and experimental methods, this is believed to be an ideal strategy for determining the band alignment in metal oxide heterojunction systems.