Miracle-dz/UniFAR-FTUs huggingface dataset:
Sperm metabolism of the telost fishes Chalcalburnus chalcoides and Oncorhynchus mykiss and its relation to motility and viability.

In the teleost fish Chalcalburnus chalcoides (Cyprinidae) the influence of metabolic inhibitors, substrates, coenzymes, and oxygen concentrations on spermatozoal parameters during motility and during immotile incubation was studied, the respiration rate was characterized, representative metabolite levels were measured, and the results were compared with Oncorhynchus mykiss (Salmonidae). In Chalcalburnus chalcoides the sperm motility rate, the average path swimming velocity, the motility duration, and the viability of immotile semen were significantly reduced in the presence of inhibitors of respiration (potassium cyanide, 2.4-dinitrophenol, atractyloside). Anaerobic conditions (<1 mg O(2)/liter) and inhibition of the tricarboxylic acid cycle by malonate and >7.5 mmol/liter succinate had similar effects on the sperm motility parameters and on the viability of immotile spermatozoa. Pyruvate and coenzyme A (an acyl-group carrier during oxidative carboxylation of pyruvate) prolonged the duration of sperm motility and the viability of immotile incubated spermatozoa, and also increased the spermatozoal respiration rate. Glucose levels significantly decreased during motility and during immotile storage and, under anaerobic conditions, the levels of lactate increased indicating that pyruvate derived from glycolysis. The respiration rate and the glycolytic rate significantly increased during motility. Therefore oxidative phosphorylation, tricarboxylic acid cycle, and aerobic glycolysis are central energy-supplying pathways for spermatozoa of Chalcalburnus chalcoides. The stimulatory effect of pyruvate and coenzyme A indicated that glycolysis is a rate-controlling pathway. Similar results were obtained for Oncorhynchus mykiss with the only exception that the stimulatory effect of coenzyme A was more significant than the stimulatory effect of pyruvate. When the sperm motility-activating saline solutions were optimized in aspects of energy supply, ionic composition, and osmolality, about 50% of the motile spermatozoa swam progressively (>20 mm/sec) for about 3 min in Chalcalburnus chalcoides and in Oncorhynchus mykiss. About 20% swam progressively for >2 hr in Chalcalburnus chalcoides and for >30 min in Oncorhynchus mykiss. J. Exp. Zool. 284:454-465, 1999.
Tuple relations and topologies on function spaces

In [7] some results concerning S-splitting, S-jointly continuous, D-splitting and D-jointly continuous topologies are considered, where S and D are the Sierpinski space and the double-point space, respectively. Here we generalize these results replacing the spaces S and D by any finite space. 2000 AMS Classification: 54C35.
A relationship between behavioral choice and the visual responses of neurons in macaque MT

Abstract We have previously documented the exquisite motion sensitivity of neurons in extrastriate area MT by studying the relationship between their responses and the direction and strength of visual motion signals delivered to their receptive fields. These results suggested that MT neurons might provide the signals supporting behavioral choice in visual discrimination tasks. To approach this question from another direction, we have now studied the relationship between the discharge of MT neurons and behavioral choice, independently of the effects of visual stimulation. We found that trial-to-trial variability in neuronal signals was correlated with the choices the monkey made. Therefore, when a directionally selective neuron in area MT fires more vigorously, the monkey is more likely to make a decision in favor of the preferred direction of the cell. The magnitude of the relationship was modest, on average, but was highly significant across a sample of 299 cells from four monkeys. The relationship was present for all stimuli (including those without a net motion signal), and for all but the weakest responses. The relationship was reduced or eliminated when the demands of the task were changed so that the directional signal carried by the cell was less informative. The relationship was evident within 50 ms of response onset, and persisted throughout the stimulus presentation. On average, neurons that were more sensitive to weak motion signals had a stronger relationship to behavior than those that were less sensitive. These observations are consistent with the idea that neuronal signals in MT are used by the monkey to determine the direction of stimulus motion. The modest relationship between behavioral choice and the discharge of any one neuron, and the prevalence of the relationship across the population, make it likely that signals from many neurons are pooled to form the data on which behavioral choices are based.
Characteristics of Lipo-Oligosaccharide Loci of Campylobacter jejuni Isolates Associated with Guillain-Barré Syndrome from Hebei, China

Ganglioside mimicry by C.jejuni lipo-oligosaccharides (LOS) could induce the production of autoantibodies against gangliosides and the development of Guillain-Barré syndrome (GBS). The LOS biosynthesis region exhibits significant variation with different strains. Using PCR amplifications of genes from published LOS loci and sequencing the LOS biosynthesis loci, the eight GBS-associated C. jejuni strains from HeBei could be classified into four classes. The expression of sialylated LOS structures (class A) or non-sialylated LOS structures(class F, H and P) in the C. jejuni LOS is considered to be two different factors for the induction of GBS.
Low separation rank covariance estimation using Kronecker product expansions

This paper presents a new method for estimating high dimensional covariance matrices. Our method, permuted rank-penalized least-squares (PRLS), is based on Kronecker product series expansions of the true covariance matrix. Assuming an i.i. d. Gaussian random sample, we establish high dimensional rates of convergence to the true covariance as both the number of samples and the number of variables go to infinity. For covariance matrices of low separation rank, our results establish that PRLS has significantly faster convergence than the standard sample covariance matrix (SCM) estimator. In addition, this framework allows one to tradeoff estimation error for approximation error, thus providing a scalable covariance estimation framework in terms of separation rank, an analog to low rank approximation of covariance matrices [1]. The MSE convergence rates generalize the high dimensional rates recently obtained for the ML Flip-flop algorithm [2], [3].
Static and dynamic analysis: synergy and duality

This paper presents two sets of observations relating static and dynamic analysis. The first concerns synergies between static and dynamic analysis. Wherever one is utilized, the other may also be applied, often in a complementary way, and existing analyses should inspire different approaches to the same problem. Furthermore, existing static and dynamic analyses often have very similar structure and technical approaches. The second observation is that some static and dynamic approaches are similar in that each considers, and generalizes from, a subset of all possible execu-
Energy efficient neighborhood maintenance and medium access with Wake on Idle

The main idea of this paper is to rely only on analog channel sensing to provide integrated neighborhood maintenance and medium access control in a single mechanism for energy constrained low-power wireless sensor nodes. We propose Wake on Idle, a solution that can be implemented in the radio chip and provides these services without relying on the processor when the stable state is reached. No digital decoding is needed as we use pseudo random sequences to identify analog beacon positions in time and assess neighbors presence. Additionally, we use a code violation principle to provide medium access, ensuring that when a node wants to transmit data to a neighbor, the latter will be listening. We analyze this proposition, give some simulation results, and also provide extensive experimentation results based on an implementation running on two different hardware platforms and showing the concept to be valid, although some adaptation was needed to run on off-the-shelf radios. Even with this far from optimal initial implementation we can reach below 0.1% duty cycles for signaling periods on the order of minutes.
Reciprocal Regulation of AKT and MAP Kinase Dictates Virus-Host Cell Fusion

ABSTRACT Viruses of the Paramyxoviridae family bind to their host cells by using hemagglutinin-neuraminidase (HN), which enhances fusion protein (F)-mediated membrane fusion. Although respiratory syncytial virus and parainfluenza virus 5 of this family are suggested to trigger host cell signaling during infection, the virus-induced intracellular signals dictating virus-cell fusion await elucidation. Using an F- or HN-F-containing reconstituted envelope of Sendai virus, another paramyxovirus, we revealed the role and regulation of AKT1 and Raf/MEK/ERK cascades during viral fusion with liver cells. Our observation that extracellular signal-regulated kinase (ERK) activation promotes viral fusion via ezrin-mediated cytoskeletal rearrangements, whereas AKT1 attenuates fusion by promoting phosphorylation of F protein, indicates a counteractive regulation of viral fusion by reciprocal activation of AKT1 and mitogen-activated protein kinase (MAPK) cascades, establishing a novel conceptual framework for a therapeutic strategy.
Cost-Aware Pre-Training for Multiclass Cost-Sensitive Deep Learning

Deep learning has been one of the most prominent machine learning techniques nowadays, being the state-of-the-art on a broad range of applications where automatic feature extraction is needed. Many such applications also demand varying costs for different types of mis-classification errors, but it is not clear whether or how such cost information can be incorporated into deep learning to improve performance. In this work, we propose a novel cost-aware algorithm that takes into account the cost information into not only the training stage but also the pre-training stage of deep learning. The approach allows deep learning to conduct automatic feature extraction with the cost information effectively. Extensive experimental results demonstrate that the proposed approach outperforms other deep learning models that do not digest the cost information in the pre-training stage.
Application of dynamic mechanical analysis for the study of the interfacial region in carbon fiber/epoxy composite materials

The application of dynamic mechanical analysis (DMA) for quantifying interfacial interactions in composites is briefly reviewed. Carbon fiber/epoxy composites with fiber volume fractions of 12, 17, 38 and 61 vol% were subjected to flexural deformation on a Dupont DMA 983 instrument. The dependencies of dynamic mechanical properties of the composites on experimental parameters such as oscillation mode, amplitude, frequency, and temperature were investigate. As opposed to the storage modulus, the loss modulus is found to be sensitive to all parameters. In a fixed multiple frequency mode, the loss modulus of the composites increases with oscillation amplitude and decreases with frequency and the number of tests. The information produced in the resonant mode is more reproducible. An additional damping at the interfaces, apart from those of the constituents, suggests a poor interface adhesion in these composites. A linear relationship between the excess damping at the interfaces and the fiber volume fraction shows a similar interface quality for these composites having different fiber volume fractions. The detection of interfacial properities was found to be more sensitive in the flexural deformation mode than in the torsional mode. At temperatures higher than the glass transition temperature of the matrix, the effective volume fraction of the matrix is reduced. Such a reduction can be interpreted from the mismatch of thermal expansion of the matrix and the fibers.
Synchronous and asynchronous A-D conversion

Analog-digital (A-D) converters with a fixed conversion time are subject to errors due to metastability. It is shown that an asynchronous converter in which the conversion time is not bounded is faster, on average, than the synchronous design. Real-time applications require the data to be produced within a fixed time, and failures may occur with the long conversion times that can arise with fully asynchronous converters. For these applications, we show that an internally asynchronous bounded time converter is both faster and more reliable than a synchronous converter.
The nucleoporin-like protein NLP1 (hCG1) promotes CRM1-dependent nuclear protein export

Translocation of transport complexes across the nuclear envelope is mediated by nucleoporins, proteins of the nuclear pore complex that contain phenylalanine-glycine (FG) repeats as a characteristic binding motif for transport receptors. CRM1 (exportin 1), the major export receptor, forms trimeric complexes with RanGTP and proteins containing nuclear export sequences (NESs). We analyzed the role of the nucleoporin-like protein 1, NLP1 (also known as hCG1 and NUPL2) in CRM1-dependent nuclear transport. NLP1, which contains many FG repeats, localizes to the nuclear envelope and could also be mobile within the nucleus. It promotes the formation of complexes containing CRM1 and RanGTP, with or without NES-containing cargo proteins, that can be dissociated by RanBP1 and/or the cytoplasmic nucleoporin Nup214. The FG repeats of NLP1 do not play a major role in CRM1 binding. Overexpression of NLP1 promotes CRM1-dependent export of certain cargos, whereas its depletion by small interfering RNAs leads to reduced export rates. Thus, NLP1 functions as an accessory factor in CRM1-dependent nuclear protein export.
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires

The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20%, 95% confidence interval = 11-33%) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejroë, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens.
Displacement cascades in Gd2Ti2O7 and Gd2Zr2O7: a molecular dynamics study

We report molecular dynamics simulations of the production of radiation cascades in two pyrochlore compounds that have been proposed as possible materials for high level radioactive waste storage. There are clear differences between the two systems that support the results of recent high energy ion bombardment experiments, in which pyrochlores were increasingly radiation resistant with increasing Zr content.
Sustained Benefit at 2 Years of Primary Femoropopliteal Stenting Compared With Balloon Angioplasty With Optional Stenting

Background— Primary stenting with self-expanding nitinol stents of the superficial femoral artery yielded improved morphological and clinical results compared with balloon angioplasty with optional stenting until 12 months in a randomized controlled trial. We now report 2-year data on restenosis and clinical outcomes of these patients. Methods and Results— Of 104 patients with chronic limb ischemia and superficial femoral artery obstructions, 98 (94%) could be followed up until 2 years after intervention for occurrence of restenosis (>50%) by duplex ultrasound and for clinical and hemodynamic outcome by treadmill walking distance and ankle brachial index. Restenosis rates at 2 years were 45.7% (21 of 46) versus 69.2% (36 of 52) in favor of primary stenting compared with balloon angioplasty with optional secondary stenting by an intention-to-treat analysis (P=0.031). Consistently, stenting (whether primary or secondary; n=63) was superior to plain balloon angioplasty (n=35) with respect to the occurrence of restenosis (49.2% versus 74.3%; P=0.028) by a treatment-received analysis. Clinically, patients in the primary stent group showed a trend toward better treadmill walking capacity (average, 302 versus 196 m; P=0.12) and better ankle brachial index values (average, 0.88 versus 0.78; P=0.09) at 2 years, respectively. Reintervention rates tended to be lower after primary stenting (17 of 46 [37.0%] versus 28 of 52 [53.8%]; P=0.14). Conclusions— At 2 years, primary stenting with self-expanding nitinol stents for the treatment of superficial femoral artery obstructions yields a sustained morphological benefit and a trend toward clinical benefit compared with balloon angioplasty with optional stenting.
Pyrogenic cytokines injected into the rat cerebral ventricle induce cyclooxygenase‐2 in brain endothelial cells and also upregulate their receptors

Peripheral immunological insults induce interleukin (IL)‐1β and IL‐6 in the brain. To elucidate the mechanism(s) of fever evoked by these brain‐derived cytokines, and possible interactions between them, we examined in rats: (i) whether cyclooxygenase‐2 is responsible for fever evoked by central injection of these cytokines; (ii) if so, where in the brain cyclooxygenase‐2 is induced; (iii) where the receptors for these cytokines are located; and (iv) how the expression of these receptors is influenced by the cytokines. Intracerebroventricular injection of these cytokines evoked fever that was suppressed by a cyclooxygenase‐2 inhibitor. Brain endothelium was the site of cyclooxygenase‐2 induction by these cytokines. IL‐1 receptor (IL‐1R) was constitutively expressed in brain endothelium, and its mRNA was further upregulated by either cytokine. IL‐6R mRNA was constitutively expressed in the cerebral cortex, and was newly induced in as yet unidentified cells in brain blood vessels by either cytokine. Messenger RNAs for cyclooxygenase‐2, IL‐1R, and IL‐6R were often observed in the same blood vessels. These results suggest that COX‐2 induced in brain endothelium is, at least in part, involved in the fever evoked by these cytokines, and that one possible interaction between these two cytokines is mutual upregulation of their receptors in the endothelium or perivascular cells, resulting in augmentation of their actions.
Decreased serum BDNF levels in patients with epileptic and psychogenic nonepileptic seizures.

To the Editor: LaFrance et al.1 reported lower plasma brain-derived neurotrophic factor (BDNF) levels in patients with epileptic seizures (ES) and psychogenic nonepileptic seizures (PNES) than healthy controls (HCs). Reduced BDNF in the plasma may be used to differentiate patients with ES and PNES from HCs, and probably from malingerers. However, the small sample size in this study is concerning. Gender and age differences should be considered. Due to the small sample size, stratified analysis seems unlikely. Plasma levels of BDNF vary between different phases of the menstrual cycle.2 It has also been shown that increasing age was associated with reduced levels of plasma BDNF.3 The gender differences and Beck Depression Inventory II (BDI-II) score may partially explain the differences of plasma BDNF levels between patients with ES or PNES and HCs. Although the authors stated that seizure frequency and temporal proximity did not influence the BDNF levels in either the PNES or ES group, the time point to obtain the blood samples was not consistent. Multiple studies have confirmed increased expression of BDNF after seizures.4 Confounders may have influenced the data interpretation. Determinants in the measurement of plasma BDNF include fasting/nonfasting state of blood draw, earlier/later measurement, and sample storage time.5 Studies on these levels should correct for the time of blood withdrawal and storage. In this study, the HCs were recruited from university staff and student volunteers. It is possible that the samples from the HCs were stored less time than the cohort, which may explain the higher levels of BDNF in the plasma of HCs compared to patients with ES or PNES.
The Ecology of Individuals: Incidence and Implications of Individual Specialization

Most empirical and theoretical studies of resource use and population dynamics treat conspecific individuals as ecologically equivalent. This simplification is only justified if interindividual niche variation is rare, weak, or has a trivial effect on ecological processes. This article reviews the incidence, degree, causes, and implications of individual‐level niche variation to challenge these simplifications. Evidence for individual specialization is available for 93 species distributed across a broad range of taxonomic groups. Although few studies have quantified the degree to which individuals are specialized relative to their population, between‐individual variation can sometimes comprise the majority of the population’s niche width. The degree of individual specialization varies widely among species and among populations, reflecting a diverse array of physiological, behavioral, and ecological mechanisms that can generate intrapopulation variation. Finally, individual specialization has potentially important ecological, evolutionary, and conservation implications. Theory suggests that niche variation facilitates frequency‐dependent interactions that can profoundly affect the population’s stability, the amount of intraspecific competition, fitness‐function shapes, and the population’s capacity to diversify and speciate rapidly. Our collection of case studies suggests that individual specialization is a widespread but underappreciated phenomenon that poses many important but unanswered questions.