Miracle-dz/UniFAR-FTUs huggingface dataset:
Container-based microservice architecture for cloud applications

Cloud Environment allows enterprises to scale their application on demand. Microservice design is a new paradigm for cloud application development which is gaining popularity due to its granular approach and loosely coupled services unlike monolithic design with single code base. Applications developed using microservice design results in better scaling and gives extended flexibility to the developers with minimum cost. In this paper, first, different challenges in deployment and continuous integration of microservices are analyzed. To overcome these challenges, later, an automated system is proposed and designed which helps in deployment and continuous integration of microservices. Containers are recently heavily used in deploying the applications as they are easy to manage and lightweight when compared to traditional Virtual Machines (VMs). We have deployed the proposed microservices architecture on the docker containers and tested using a social networking application as case study. Finally, the results are presented and the performance of monolithic and microservice approach is compared using various parameters such as response time, throughput, deployment time etc. Results show that application developed using microservice approach and deployed using the proposed design reduce the time and effort for deployment and continuous integration of the application. Results also shows that microservice based application outperform monolithic design because of its low response time and high throughput.
Control of long-distance cell-to-cell communication and autophagosome transfer in squamous cell carcinoma via tunneling nanotubes

Tunneling nanotubes (TnTs) are thin channels that temporally connect nearby cells allowing the cell-to-cell trafficking of biomolecules and organelles. The presence or absence of TnTs in human neoplasms and the mechanisms of TnT assembly remains largely unexplored. In this study, we have identified TnTs in tumor cells derived from squamous cell carcinomas (SCC) cultured under bi-dimensional and tri-dimensional conditions and also in human SCC tissues. Our study demonstrates that TnTs are not specific of epithelial or mesenchymal phenotypes and allow the trafficking of endosomal/lysosomal vesicles, mitochondria, and autophagosomes between both types of cells. We have identified focal adhesion kinase (FAK) as a key molecule required for TnT assembly via a mechanism involving the MMP-2 metalloprotease. We have also found that the FAK inhibitor PF-562271, which is currently in clinical development for cancer treatment, impairs TnT formation. Finally, FAK-deficient cells transfer lysosomes/autophagosomes to FAK-proficient cells via TnTs which may represent a novel mechanism to adapt to the stress elicited by impaired FAK signaling. Collectively, our results strongly suggest a link between FAK, MMP-2, and TnT, and unveil new vulnerabilities that can be exploited to efficiently eradicate cancer cells.
Kita Driven Expression of Oncogenic HRAS Leads to Early Onset and Highly Penetrant Melanoma in Zebrafish

Background Melanoma is the most aggressive and lethal form of skin cancer. Because of the increasing incidence and high lethality of melanoma, animal models for continuously observing melanoma formation and progression as well as for testing pharmacological agents are needed. Methodology and Principal Findings Using the combinatorial Gal4 –UAS system, we have developed a zebrafish transgenic line that expresses oncogenic HRAS under the kita promoter. Already at 3 days transgenic kita-GFP-RAS larvae show a hyper-pigmentation phenotype as earliest evidence of abnormal melanocyte growth. By 2–4 weeks, masses of transformed melanocytes form in the tail stalk of the majority of kita-GFP-RAS transgenic fish. The adult tumors evident between 1–3 months of age faithfully reproduce the immunological, histological and molecular phenotypes of human melanoma, but on a condensed time-line. Furthermore, they show transplantability, dependence on mitfa expression and do not require additional mutations in tumor suppressors. In contrast to kita expressing melanocyte progenitors that efficiently develop melanoma, mitfa expressing progenitors in a second Gal4-driver line were 4 times less efficient in developing melanoma during the three months observation period. Conclusions and Significance This indicates that zebrafish kita promoter is a powerful tool for driving oncogene expression in the right cells and at the right level to induce early onset melanoma in the presence of tumor suppressors. Thus our zebrafish model provides a link between kita expressing melanocyte progenitors and melanoma and offers the advantage of a larval phenotype suitable for large scale drug and genetic modifier screens.
endrochronological dating of the Graves Mill grist mill , Madison ounty , Virginia , USA

Historical dendroarchaeology uses annual tree-ring widths varied by climate to crossdate structures of potential historical significance. In the Southeastern U.S. Piedmont specifically, crossdating these structures can lead to a broader historical interpretation during and after the colonial era. Our study used historical dendroarchaeology to date the Graves Mill grist mill located in Madison County along the western edge of the Virginia Piedmont. The mill and mill site reflect a regional trend in eighteenth century land use as agriculture transitioned from tobacco to wheat production in response to demand from international markets, as well as the adverse impact of tobacco cultivation on piedmont soils. Mills in this region have also been subject to flooding events from storms, with events recorded in the colonial era to a recent record flood in 1995, as well as re-building with changes in mill technologies through time. In an effort to date this structure as part of its restoration, and to verify the repeatability of this dating, ring widths from six timbers from the upper stories were analyzed independently at tree-ring laboratories at the University of Tennessee, Knoxville and Rider University. Both laboratories identified an 1816 cutting date for these samples, supporting documentary evidence that the mill likely underwent modifications over time after it was initially constructed in the eighteenth century. The consistent crossdating of these samples highlights the usefulness of dendrochronology as a research tool in the Southeastern U.S. for interpreting historical structures and their surrounding historical context. © 2016 Elsevier GmbH. All rights reserved.
A REVIEW ON THE IMPACT OF THE ENVIRONMENTAL ADVERSITIES ON VARIOUS DEVELOPMENTAL DISORDERS OF BRAIN IN CHILDREN Review Article

Scientific researches over the last 30 years in defining the role of various toxins and stresses during the utero and postnatal phase, malnutrition, brain injuries and selective food deficiencies have shown enough evidence of their role in neurodevelopmental disorders. The effect of environment on various developmental disorders such as Attention deficit hyperactivity disorder (ADHD), autism spectrum and learning disabilities has paved the way for better understanding of genetic influences. Several types of genetic and environmental adversities indicate that neurodevelopmental disorders suggest a causal role. There is marked variation in the susceptibility of individuals to these adversities. Thus their vulnerability to the disease could influence the extent they are prone to that disease. The aim of this review is to bring together various aspects of environmental factors and genetic susceptibilities and summarize their toxic effects towards neurodevelopment in children.
Genomics and host specialization of honey bee and bumble bee gut symbionts

Significance Gut microbes are increasingly recognized as influential components of animal biology. Genomic, mechanistic, and evolutionary aspects of gut symbiont specialization remain understudied, however, largely due to the complexity of gut communities, especially in vertebrate systems. We show that the simple microbiota of eusocial bees exhibits host specificity and that coresident species in the bee gut possess complementary capabilities for energy metabolism, implying their occupancy in distinct ecological niches. In addition, coresidence in the gut of a host species results in horizontal exchange of genes between unrelated symbionts. Strains in different hosts have diverged, and honey bee symbionts are evolutionarily and functionally distinct from their bumble bee counterparts, indicating that gut symbionts may be critical elements in biological differences among bee species. Gilliamella apicola and Snodgrassella alvi are dominant members of the honey bee (Apis spp.) and bumble bee (Bombus spp.) gut microbiota. We generated complete genomes of the type strains G. apicola wkB1T and S. alvi wkB2T (isolated from Apis), as well as draft genomes for four other strains from Bombus. G. apicola and S. alvi were found to occupy very different metabolic niches: The former is a saccharolytic fermenter, whereas the latter is an oxidizer of carboxylic acids. Together, they may form a syntrophic network for partitioning of metabolic resources. Both species possessed numerous genes [type 6 secretion systems, repeats in toxin (RTX) toxins, RHS proteins, adhesins, and type IV pili] that likely mediate cell–cell interactions and gut colonization. Variation in these genes could account for the host fidelity of strains observed in previous phylogenetic studies. Here, we also show the first experimental evidence, to our knowledge, for this specificity in vivo: Strains of S. alvi were able to colonize their native bee host but not bees of another genus. Consistent with specific, long-term host association, comparative genomic analysis revealed a deep divergence and little or no gene flow between Apis and Bombus gut symbionts. However, within a host type (Apis or Bombus), we detected signs of horizontal gene transfer between G. apicola and S. alvi, demonstrating the importance of the broader gut community in shaping the evolution of any one member. Our results show that host specificity is likely driven by multiple factors, including direct host–microbe interactions, microbe–microbe interactions, and social transmission.
Mass spectral determination of fasting tear glucose concentrations in nondiabetic volunteers.

BACKGROUND There is considerable disagreement regarding the concentration of glucose in tears and its relationship to the concentration in blood. Improved sampling and analysis methods may resolve these discrepancies and possibly provide a basis for in situ tear glucose sensors. METHODS We used liquid chromatography (LC) with electrospray ionization mass spectrometry (ESI-MS) to determine glucose in 1-muL tear fluid samples obtained from 25 fasting study participants. Tear fluid was collected with microcapillaries and a slitlamp microscope. RESULTS The median (range) of fasting tear glucose concentrations was 28 (7-161) micromol/L or 0.50 (0.13-2.90) mg/dL. The SD of tear glucose measurements for individuals varied linearly with the mean tear glucose concentration and was approximately half of the mean. We found no significant difference in tear glucose concentrations between contact lens users and nonusers (P = 0.715). We observed significant correlations between fasting blood and tear glucose concentrations (R = 0.50, P = 0.01). CONCLUSIONS Our tear fluid collection and analysis method enables reliable measurement of equilibrium, fasting tear glucose concentrations. These concentrations are lower than those previously reported for nondiabetic persons. Larger population studies are required to determine correlations between blood and tear glucose concentrations and to determine the utility of contact lens-based sensors for the monitoring of diabetes. Our methods are applicable for study of other tear fluid analytes and may prove useful for monitoring other disease states.
INVESTIGATION OF VOCATIONAL HIGH-SCHOOL STUDENTS' COMPUTER ANXIETY

With the advent of the computer technologies, we are increasingly encountering these technologies in every field of life. The fact that the computer technology is so much interwoven with the daily life makes it necessary to investigate certain psychological attitudes of those working with computers towards computers. As this study is limited to the impact of technology on education, anxiety, out of certain psychological attitudes, was investigated within it. The investigation was on the vocational high-school students’ computer-related anxiety, and as a data collection instrument, the Computer Anxiety Scale was used. The Computer Anxiety Scale was conducted on 265 students receiving education at various departments in the Tunceli Vocational School of Tunceli University. It was observed as a result that gender is a variable that did not make any meaningful difference for the computer anxiety, while the type of education received, grade, receiving computer education and having a computer may mean meaningful differences in terms of various sub-factors and the whole scale itself. It is necessary to determine the level of computer anxiety, and keep it under control while receiving computer education or while learning with computers.
Salvage surgery in post-chemoradiation laryngeal and hypopharyngeal carcinoma: outcome and review

SUMMARY Our objective was to evaluate recurrence patterns of hypopharyngeal and laryngeal carcinoma after chemoradiation and options for salvage surgery, with special emphasis on elderly patients. In a retrospective study all patients who underwent chemoradiation for hypopharyngeal and laryngeal carcinoma in a tertiary care academic center from 1990 through 2010 were evaluated. Primary outcome measures were the survival and complication rates of patients undergoing salvage surgery, especially in elderly patients. Secondary outcome measures were the predictors for salvage surgery for patients with locoregional recurrence after failed chemoradiotherapy. A review of the literature was performed. Of the 136 included patients, 60 patients had recurrent locoregional disease, of whom 22 underwent salvage surgery. Fifteen patients underwent a total laryngectomy with neck dissection(s) and 7 neck dissection without primary tumour surgery. Independent predictors for salvage surgery within the group of 60 patients with recurrent disease, were age under the median of 59 years (p = 0.036) and larynx vs. hypopharynx (p = 0.002) in multivariate analyses. The complication rate was 68% (14% major and 54% minor), with fistulas in 23% of the patients. Significantly more wound related complications occurred in patients with current excessive alcohol use (p = 0.04). Five-year disease free control rate of 35%, overall survival rate of 27% and disease specific survival rate of 35% were found. For the 38 patients who were not suitable for salvage surgery, median survival was 12 months. Patients in whom the tumour was controlled had a 5-year overall survival of 70%. In patients selected for salvage surgery age was not predictive for complications and survival. In conclusion, at two years follow-up after chemoradiation 40% of the patients were diagnosed with recurrent locoregional disease. One third underwent salvage surgery with 35% 5-year disease specific survival and 14% major complications. Older patients selected for salvage surgery had a similar complication rate and survival as younger patients.
The Relationship of Hardiness and Some Other Relevant Variables to College Performance

Although it has emerged as a factor in performance effectiveness in both working adults and college students, hardiness has also been shown to be related to other factors that may well also be related to performance effectiveness. Consequently, attempts need to be made to determine whether the relationship to performance of hardiness is anything more than a mere reflection of its relationship to these other factors. This approach, which was begun in the comparison of the relative role of hardiness, optimism, and religiosity in performance effectiveness, is continued in the present study. Specifically, the relationship to grade point average of hardiness and related variables is compared. The related variables involve attitudes toward school and a sense of life’s meaning and one’s well-being. The results confirm the expectation that hardiness is a central factor in school performance.
Observation and Analysis of Expert Teaching in Beginning Band

The purpose of this study was to identify common characteristics among expert teachers in beginning band settings. Three subjects were observed across three consecutive classes for a total of approximately 370 minutes. Data sources included on-site and videotape observations, field notes, analysis of instructional goals, and frequency and duration data collected on specified teacher and student behaviors. Organizing field notes using thematic headings revealed that the teachers were proactive in managing student behavior, they prioritized the development of characteristic tones via fundamental concepts of breathing, embouchure, and posture and instrument carriage, and they provided musical models on wind instruments. Twenty-five rehearsal frames were identified and analyzed to determine their instructional targets and the frequency and duration of specified teacher and student behaviors. Pitch Accuracy, Multiple Targets, and Posture/Instrument Carriage were the most frequently observed rehearsal frame target categories. Instructional pace differed from what has been reported in middle school, high school and college settings; subjects talked and modeled for greater proportions, longer durations and lower rates, while students performed for smaller proportions, shorter durations and lower rates than what has been observed in other settings.
Determination of differentially regulated proteins upon proteasome inhibition in AML cell lines by the combination of large‐scale and targeted quantitative proteomics

The ubiquitin‐proteasome pathway (UPP) plays a critical role in the degradation of proteins implicated in cell cycle control, signal transduction, DNA damage response, apoptosis and immune response. Proteasome inhibitors can inhibit the growth of a broad spectrum of human cancer cells by altering the balance of intracellular proteins. However, the targets of these compounds in acute myeloid leukemia (AML) cells have not been fully characterized. Herein, we combined large‐scale quantitative analysis by SILAC‐MS and targeted quantitative proteomic analysis in order to identify proteins regulated upon proteasome inhibition in two AML cell lines displaying different stages of maturation: immature KG1a cells and mature U937 cells. In‐depth data analysis enabled accurate quantification of more than 7000 proteins in these two cell lines. Several candidates were validated by selected reaction monitoring (SRM) measurements in a large number of samples. Despite the broad range of proteins known to be affected by proteasome inhibition, such as heat shock (HSP) and cell cycle proteins, our analysis identified new differentially regulated proteins, including IL‐32, MORF family mortality factors and apoptosis inducing factor SIVA, a target of p53. It could explain why proteasome inhibitors induce stronger apoptotic responses in immature AML cells.
Expression of the Voltage-Gated Sodium Channel NaV1.5 in the Macrophage Late Endosome Regulates Endosomal Acidification1

Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to examine the role of intracellular voltage-gated sodium channels in macrophage function. We demonstrate that the cardiac voltage-gated sodium channel, NaV1.5, is expressed on the late endosome, but not the plasma membrane, in a human monocytic cell line, THP-1, and primary human monocyte-derived macrophages. Although the neuronal channel, NaV1.6, is also expressed intracellularly, it has a distinct subcellular localization. In primed cells, NaV1.5 regulates phagocytosis and endosomal pH during LPS-mediated endosomal acidification. Activation of the endosomal channel causes sodium efflux and decreased intraendosomal pH. These results demonstrate a functionally relevant intracellular voltage-gated sodium channel and reveal a novel mechanism to regulate macrophage endosomal acidification.
Ocular cysticercosis in a 32-year-old man in Abuja: ultrasonic features as an aid in diagnosis

We report the case of a 32-year-old man suffering from intraocular cysticercosis, with special emphasis on the use of B-scan ultrasound in the diagnosis and management of the condition. An 8000 B-Scan Scanmate was used to obtain the ultrasound images. The patient had worked on a pig farm a few years before presentation. He presented with shadows seen in the right eye. Binocular indirect ophthalmoscopy revealed that he had a choroidal detachment in the right eye inferotemporally. B-scan ultrasound revealed a subretinal subchoroidal cyst with a thick wall containing well defined intracystic echogenic entities representing scolices, and an associated retinal detachment. These findings appear to be pathognomonic. Excision of the cyst through a trans-scleral approach revealed a yellowish serous fluid, with scolices of cysticercus later confirmed histologically. B-scan ultrasound is extremely useful in the diagnosis of ocular cysticercosis and the findings can be pathognomonic.
Effect of land use on the density of nitrifying and denitrifying bacteria in the Colombian Coffee Region Efecto del uso de suelo sobre la densidad de bacterias nitrificantes y desnitrificantes en la Ecorregión Cafetera Colombiana

RESUMEN Soil microbial communities involved in the cycling of nitrogen (N) are essential to maintaining and improving soil fertility, productivity and functionality of natural and agricultural ecosystems. However, some compounds generated during the metabolic processes performed by nitrifying (NB) and denitrifying (DB) bacteria are associated with the production of greenhouse gases, groundwater pollution and acidification. Therefore, the study of these bacteria is essential for economic and environmental sustainability. This study evaluated the effect of different land uses in two river basins (La Vieja and Otun) on NB and DB densities. Two sampling events (SE) were conducted by selecting the most representative land uses. Physicochemical (T °, pH, moisture and nitrate) and microbiological properties (NB and DB densities) were evaluated. In both SEs, significantly higher densities of NB and DB were observed in the land uses: pasture, guadua (DB only) and unshaded coffee (La Vieja) and onion (Otun). These land uses, excluding guadua, are dependent on nitrogen fertilizers, which together with the activities of grazing livestock on pastures may lead to greater availability of substrates for the NB. The use of agricultural machinery and overgrazing in pasture and onion uses generate compacted soil and other physical disturbances, encouraging the growth of DB. Forests had the lowest densities of NB and DB possibly due to a reduced availability of N and the releasing of allelopathic compounds from certain plants. Finally, the densities of ammonium-oxidizing bacteria had the greatest differences between the land uses evaluated, demonstrating its high sensitivity to agricultural management practices and livestock. We suggest that changes in the abundance of this community could serve as a relevant and cost-effective bioin dicator for soil monitoring.