Miracle-dz/UniFAR-FTUs huggingface dataset:
In vivo confocal microscopy in the normal corneas of cats, dogs and birds.

OBJECTIVE To evaluate the applicability of in vivo confocal microscopy (IVCM) in veterinary ophthalmology and analyze the morphology of living, healthy cornea. ANIMALS EXAMINED: Thirty-seven dogs, 34 cats and five birds. PROCEDURE Various corneal sublayers were visualized in the central region using an in vivo confocal corneal microscope (HRTII/RCM). RESULTS An investigation method was developed and adapted for use on animals with varying skull forms and eye positions. Real-time images of the epithelial cells, the corneal stroma and the endothelial layer were obtained. The corneal stromal nerve trunks and the subepithelial and basal epithelial nerve plexus were visualized. In dogs, full corneal thickness (FCT) was 585 +/- 79 microm (mean +/- SD) and endothelial cell density (ECD) 3175 +/- 776 cells/mm(2) (mean +/- SD). In cats, FCT was 592 +/- 80 microm and ECD 2846 +/- 403 cells/mm(2). There were no significant differences between canine and feline FCT and ECD and no morphologic differences could be seen between dogs and cats. The bird images revealed a number of structural differences. CONCLUSION Noninvasive IVCM allows accurate detection of corneal sublayers, corneal pachymetry, endothelial cell density and corneal innervation in various animal species. For clinical usage, patients must be under general anesthesia. The confocal images provided anatomic reference images of various healthy corneal structures in dogs, cats and birds.
Adverse effects of isoniazid preventative therapy for latent tuberculosis infection: a prospective cohort study

Introduction Isoniazid preventative therapy (IPT) is a widely used intervention for treatment of latent tuberculosis infection (LTBI), particularly in patients at high risk for reactivation. While treatment-limiting adverse effects have been well studied, few prospective studies have considered the range of adverse effects that patients may experience with IPT. Methods All patients commencing treatment for LTBI were prospectively enrolled in an ongoing database of LTBI treatment outcomes particularly related to adverse effects, treatment adherence, and treatment completion. Results Data on the first 100 patients who were prescribed IPT are presented. Fifty-six patients reported at least one adverse effect at some stage during treatment, with six experiencing at least one World Health Organization (WHO) Grade 3–4 adverse effect. Increased age was significantly associated with risk of adverse effects (odds ratio [OR] =1.05 per year; confidence interval [CI] of 1.02–1.08=95%). Eighty-five patients had documented completion of therapy locally, with ten patients ceasing IPT due to adverse effects. Discussion This report highlights a variety of somatic adverse effects that occurred in a real-world cohort of patients receiving IPT. While adverse effects were frequently identified in this study, the considerable majority were low grade and transient. Despite frequent adverse effects of LTBI in our treatment cohort, the study demonstrated high levels of treatment adherence and completion.
DISCOVERY OF TWIN WOLF-RAYET STARS POWERING DOUBLE RING NEBULAE

We have spectroscopically discovered a pair of twin, nitrogen-type, hydrogen-rich, Wolf-Rayet stars (WN8–9h) that are both surrounded by circular, mid-infrared-bright nebulae detected with the Spitzer Space Telescope and MIPS instrument. The emission is probably dominated by a thermal continuum from cool dust, but also may contain contributions from atomic line emission. There is no counterpart at shorter Spitzer/IRAC wavelengths, indicating a lack of emission from warm dust. The two nebulae are probably wind-swept stellar ejecta released by the central stars during a prior evolutionary phase. The nebulae partially overlap on the sky and we speculate on the possibility that they are in the early stage of a collision. Two other evolved massive stars have also been identified within the area subtended by the nebulae, including a carbon-type Wolf-Rayet star (WC8) and an O7–8 III–I star, the latter of which appears to be embedded in one of the larger WN8–9h nebulae. The derived distances to these stars imply that they are coeval members of an association lying 4.9 ± 1.2 kpc from Earth, near the intersection of the Galaxy's Long Bar and the Scutum-Centaurus spiral arm. This new association represents an unprecedented display of complex interactions between multiple stellar winds, outflows, and the radiation fields of evolved massive stars.
Regulation of pancreatic β-cell insulin secretion by actin cytoskeleton remodelling: role of gelsolin and cooperation with the MAPK signalling pathway

We have previously isolated two MIN6 β-cell sublines, B1, highly responsive to glucose-stimulated insulin secretion, and C3, markedly refractory (Lilla, V., Webb, G., Rickenbach, K., Maturana, A., Steiner, D. F., Halban, P. A. and Irminger, J. C. (2003) Endocrinology 144, 1368-1379). We now demonstrate that C3 cells have substantially increased amounts of F-actin stress fibres whereas B1 cells have shorter cortical F-actin. Consistent with these data, B1 cells display glucose-dependent actin remodelling whereas, in C3 cells, F-actin is refractory to this secretagogue. Furthermore, F-actin depolymerisation with latrunculin B restores glucose-stimulated insulin secretion in C3 cells. In parallel, glucose-stimulated ERK1/2 activation is greater in B1 than in C3 cells, and is potentiated in both sublines following F-actin depolymerisation. Glucose-activated phosphoERK1/2 accumulates at actin filament tips adjacent to the plasma membrane, indicating that these are the main sites of action for this kinase during insulin secretion. In addition, B1 cell expression of the calcium-dependent F-actin severing protein gelsolin is >100-fold higher than that of C3 cells. Knock-down of gelsolin reduced glucose-stimulated insulin secretion, whereas gelsolin over-expression potentiated secretion from B1 cells. Gelsolin localised along depolymerised actin fibres after glucose stimulation. Taken together, these data demonstrate that F-actin reorganization prior to insulin secretion requires gelsolin and plays a role in the glucose-dependent MAPK signal transduction that regulates β-cell insulin secretion.
Search for a Gene Expression Signature of Breast Cancer Local Recurrence in Young Women

Purpose: A gene expression signature, predictive for local recurrence after breast-conserving treatment, has previously been identified from a series of 165 young patients with breast cancer. We evaluated this signature on both another platform and an independent series, compared its performance with other published gene-sets, and investigated the gene expression profile of a larger data set. Experimental Design: Gene expression tumor profiles were obtained on 148 of the initial 165 Dutch patients and on an independent validation series of 195 French patients. Both unsupervised and supervised classifications were used to study the gene expression profile of the 343 breast cancers and to identify subgroups that differ for their risk of local recurrence. Results: The previous local recurrence signature was validated across platforms. However, when applied to the French patients, the signature did not reproduce its reported performance and did not better classify the patients than other published gene sets. Hierarchical clustering of all 343 breast cancers did not show any grouping reflecting local recurrence status. Genes related to proliferation were found differentially expressed between patients with or without local recurrence only in triple-negative tumors. Supervised classification revealed no significant gene set predictive for local recurrence or able to outperform classification based on clinical variables. Conclusions: Although the previously identified local recurrence signature was robust on another platform, we were neither able to validate it on an independent data set, nor able to define a strong gene expression classifier for local recurrence using a larger data set. We conclude that there are no significant differences in gene expression pattern in tumors from patients with and without local recurrence after breast-conserving treatment. Clin Cancer Res; 18(6); 1704–15. ©2012 AACR.
Minimal Time Problems with Moving Targets and Obstacles

We consider minimal time problems governed by nonlinear systems under general time dependant state constraints and in the two-player games setting. In general, it is known that the characterization of the minimal time function, as well as the study of its regularity properties, is a difficult task in particular when no controlability assumption is made. In addition to these difficulties, we are interested here to the case when the target, the state constraints and the dynamics are allowed to be time-dependent. We introduce a particular "reachability" control problem, which has a supremum cost function but is free of state constraints. This auxiliary control problem allows to characterize easily the backward reachable sets, and then, the minimal time function, without assuming any controllability assumption. These techniques are linked to the well known level-set approachs. Partial results of the study have been published recently by the authors in SICON. Here, we generalize the method to more complex problems of moving target and obstacle problems. Our results can be used to deal with motion planning problems with obstacle avoidance.
Cancer outcomes in low-income elders: is there an advantage to being on Medicaid?

BACKGROUND Because of reduced financial barriers, dual Medicare-Medicaid enrollment of low-income Medicare beneficiaries may be associated with receipt of definitive cancer treatment and favorable survival outcomes. METHODS We used a database developed by linking records from the Ohio Cancer Incidence Surveillance System with Medicare and Medicaid files, death certificates, and U.S. Census data. The study population included community-dwelling Medicare fee-for-service beneficiaries, age 66 years or older, with low incomes, residing in Ohio, and diagnosed with incident loco-regional breast (n=838), colorectal (n=784), or prostate cancer (n=946) in years 1997-2001. We identified as "duals" Medicare beneficiaries who were enrolled in Medicaid at least three months prior to cancer diagnosis. Multivariable logistic regression and survival models were developed to analyze the association between dual status and (1) receipt of definitive treatment; and (2) overall and disease-specific survival, after adjusting for tumor stage and patient covariates. RESULTS DUAL STATUS WAS ASSOCIATED WITH A SIGNIFICANTLY LOWER LIKELIHOOD TO RECEIVE DEFINITIVE TREATMENT AMONG COLORECTAL CANCER PATIENTS (ADJUSTED ODDS RATIO: 0.60, 95% Confidence Interval, or CI, [0.38, 0.95]), but not among breast or prostate cancer patients. Furthermore, dual status was associated with decreased overall survival among prostate cancer patients (Adjusted Hazard Ratio, or AHR, 1.45, 95% CI [1.05, 2.02]), and decreased disease-specific survival among colorectal cancer patients (AHR: 1.52 [1.05, 2.19]). CONCLUSION Enrollment of low-income Medicare beneficiaries in Medicaid is not associated with favorable treatment patterns or survival outcomes. Differences in health and functional status between community-dwelling duals and non-duals might help explain the observed disparities.
The Effects of Adolescent Victimization on Self-Concept and Depressive Symptoms

Using two waves of the Developmental Victimization Survey (DVS), this research examined the effects of different forms of child victimization on changes in self-concept in a national sample of 11- to 18-year-old youth. Specifically, we (a) assessed the independent effects of past-year sexual victimization, nonsexual child maltreatment, peer victimization, and nonvictimization adversity on changes in mastery and self-esteem, (b) investigated the effects of these stressors on levels of depressive symptoms, and (c) determined the extent to which changes in mastery and/or self-esteem mediate associations between victimization and depression. Results indicate that only sexual victimization independently reduced self-esteem, and there were no significant changes in mastery in response to victimization exposure. Declines in self-esteem partially mediated the association between past-year sexual victimization exposure and levels of depressive symptoms. Strong direct effects of each form of victimization and nonvictimization adversity on depression were also evident. Results suggest that sexual victimization experiences may have uniquely powerful effects on self-esteem that are not apparent for other types of victimization and stress.
Mucormycosis in an HIV-infected renal transplant patient: A case report and review of the literature

Patient: Female, 15 Final Diagnosis: Mucormycosis Symptoms: Lower extremity swelling • respiratory failure • short of breath Medication: — Clinical Procedure: — Specialty: Infectious Diseases Objective: Rare disease Background: Mucormycosis is an uncommonly encountered clinical syndrome in Human Immunodeficiency Virus (HIV)-infected patients. The syndrome is well described in solid organ transplant (SOT) patients, in whom it mainly causes pulmonary or rhinocerebral disease. Mucormycosis in HIV-infected patients who underwent SOT has rarely been described. Case Report: In this article, we describe an HIV-infected patient with a renal transplant who developed mucormycosis, and we review the literature. A 45-year-old African-American female with a history of HIV controlled by anti-retrovirals was admitted with shortness of breath, lower extremity swelling, and respiratory failure. Bronchoscopic results confirmed Mucor on pathology. She was treated with posaconazole and caspofungin, but her condition deteriorated. Computed axial tomography (CT) scan of the head without contrast showed multiple low attenuation lesions throughout the brain parenchyma, with the largest lesion centered in the left basal ganglia with extensive mass effect with subfalcine herniation and early transtentorial herniation with acute hydrocephalus. Even though we did not have brain tissue to make a precise diagnosis, it is likely that the central nervous system involvement in this patient was due to mucormycosis. Conclusions: In summary, we describe the case of an HIV-infected patient with renal transplant who died of disseminated mucormycosis. As the number of renal transplants in the HIV-infected population is increasing, clinicians should be aware of the possibility of disseminated mucormycosis. Early diagnosis and effective prophylaxis may alter the course of this devastating syndrome.
Grocery shopping recommendations based on basket-sensitive random walk

We describe a recommender system in the domain of grocery shopping. While recommender systems have been widely studied, this is mostly in relation to leisure products (e.g. movies, books and music) with non-repeated purchases. In grocery shopping, however, consumers will make multiple purchases of the same or very similar products more frequently than buying entirely new items. The proposed recommendation scheme offers several advantages in addressing the grocery shopping problem, namely: 1) a product similarity measure that suits a domain where no rating information is available; 2) a basket sensitive random walk model to approximate product similarities by exploiting incomplete neighborhood information; 3) online adaptation of the recommendation based on the current basket and 4) a new performance measure focusing on products that customers have not purchased before or purchase infrequently. Empirical results benchmarking on three real-world data sets demonstrate a performance improvement of the proposed method over other existing collaborative filtering models.
An Increased TREK-1–like Potassium Current in Ventricular Myocytes During Rat Cardiac Hypertrophy

Abstract: To elucidate the expression and identify the functional changes of 2 pore domain potassium channel TREK-1 during cardiac hypertrophy in rats, left ventricular hypertrophy was induced by subcutaneous injection with isoproterenol. Western blot was used to detect the expression of TREK-1 channel protein, and inside-out and whole-cell recordings were used to record TREK-1 currents. The results showed that TREK-1 protein expression in endocardium was slightly higher than that in epicardium in control left ventricles. However, it was obviously upregulated by 89.8% during hypertrophy, 2.3-fold higher than in epicardium. Mechanical stretch, intracellular acidification, and arachidonic acid could activate a TREK-1–like current in cardiomyocytes. The slope conductances of cardiac TREK-1 and CHO/TREK-1 channels were 123 ± 7 and 113 ± 17 pS, respectively. The TREK-1 inhibitor L-3-n-butylphthalide (10 &mgr;M) reduced the currents in CHO/TREK-1 cells, normal cardiomyocytes, and hypertrophic cardiomyocytes by 48.5%, 54.3%, and 55.5%, respectively. The percentage of L-3-n-butylphthalide–inhibited outward whole-cell current in hypertrophic cardiomyocytes (23.7%) was larger than that in normal cardiomyocytes (14.2%). The percentage of chloroform-activated outward whole-cell current in hypertrophic cardiomyocytes (58.3%) was also larger than normal control (40.2%). Our results demonstrated that in hypertrophic rats, TREK-1 protein expression in endocardium was specifically increased and the ratio of TREK-1 channel current in cardiac outward currents was also enhanced. TREK-1 might balance potassium ion flow during hypertrophy and might be a potential drug target for heart protection.
Support for e-Health Services Among Elderly Primary Care Patients

Background: E-health has a substantial potential to improve access to as well as support efficient and effective care for the elderly. Questions remain and must be addressed, however, regarding the challenges faced by the elderly in the use of this technology. The aim of the study was to assess the attitude (needs) and preferences of the elderly in a selected region of Poland regarding selected e-health services and the factors associated with them. Materials and Methods: The study was conducted among 286 patients over 60 years of age being served by general practitioners in southwest Poland’s Lower Silesia Province. The assessment pertaining to e-health was based on a specially designed questionnaire. Results: Nearly one-third of the study respondents had a computer at home, and 61% of these (19% of all surveyed elderly) used it. Twenty-two percent of respondents used the Internet, at least occasionally, whereas 62% used mobile phones. Approximately 41% (n = 116) of the elderly had a favorable attitude toward e-health services (labeled here as supporters) and were willing to use it if/when offered an opportunity to do so. A substantial majority (84%) of supporters expressed a desire to receive simple medical recommendations via mobile phone or a computer, although significant majorities (61% and 60%, respectively) would like to receive the results of tests by e-mail or short message service reminders for scheduled visits or prescribed medications. Slightly less than half (47%) of e-health supporters would request appointments online. Among the more important factors associated with support of e-health services were urban residence, higher education, and normal cognitive function, as well as having a computer, Internet access, or a mobile phone. Conclusions: The majority of elderly patients in this Polish community are not overly enthusiastic about using information and communications technology tools in their healthcare. Nevertheless, a substantial percentage (41%) among this group support selected e-health services.
Evaluation of screen time activities and their relationship with physical activity, overweight and socioeconomic status in children 10-12 years of age in Sanandaj, Iran: A cross-sectional study in 2015

Background: Screen time (ST), including watching television and playing electronic games are the leading cause of a growing obesity epidemic. This study aimed to evaluate ST and its association with physical activity, overweight and socioeconomic status (SES) in children 10 to 12 years of age in Sanandaj. Methods: This study was designed as a cross-sectional study, which was conducted in Sanandaj in 2015. ST and physical activity data were collected using the Modifiable Activity Questionnaire (MAQ). Overweight indices are defined based on BMI age- and gender-specific percentiles, as proposed by WHO criteria. Considering household assets data, SES was calculated using principal component analysis (PCA). The relationship between ST and different determinants was assessed using logistic regression analysis. Results: Based on the results obtained in our study, 47.28% (95% CI: 45.33-49.24) of the participants spent more than two hours a day on television and video watching and electronic games playing. People who spend greater time on ST activities, independent of their physical activities, are more susceptible to overweight and obesity (p=0.002). People in higher socioeconomic groups spent more time on watching TV and video and playing electronic games (p=0.001). There was a direct relationship between the residential area and ST (P=0.052). ST in male was found to be greater (p=0.033). In addition, ST was also lower in school-aged children whose mothers had a greater education (p=0.56). Conclusion: Based on the results of this study, it is recommended to increase the education level and knowledge of mothers and design interventions consistent with children gender and residential location so that to reduce ST and its associated outcomes in children.
Las1L Is a Nucleolar Protein Required for Cell Proliferation and Ribosome Biogenesis

ABSTRACT Ribosome biogenesis is a highly regulated process ensuring that cell growth (increase in biomass) is coordinated with cell proliferation. The formation of eukaryotic ribosomes is a multistep process initiated by the transcription and processing of rRNA in the nucleolus. Concomitant with this, several preribosomal particles, which transiently associate with numerous nonribosomal factors before mature 60S and 40S subunits are formed and exported in the cytoplasm, are generated. Here we identify Las1L as a previously uncharacterized nucleolar protein required for ribosome biogenesis. Depletion of Las1L causes inhibition of cell proliferation characterized by a G1 arrest dependent on the tumor suppressor p53. Moreover, we demonstrate that Las1L is crucial for ribosome biogenesis and that depletion of Las1L leads to inhibition of rRNA processing and failure to synthesize the mature 28S rRNA. Taken together, our data demonstrate that Las1L is essential for cell proliferation and biogenesis of the 60S ribosomal subunit.