Miracle-dz/UniFAR-FTUs huggingface dataset:
v-Jun Overrides the Mitogen Dependence of S-Phase Entry by Deregulating Retinoblastoma Protein Phosphorylation and E2F-Pocket Protein Interactions as a Consequence of Enhanced Cyclin E-cdk2 Catalytic Activity

ABSTRACT v-Jun accelerates G1 progression and shares the capacity of the Myc, E2F, and E1A oncoproteins to sustain S-phase entry in the absence of mitogens; however, how it does so is unknown. To gain insight into the mechanism, we investigated how v-Jun affects mitogen-dependent processes which control the G1/S transition. We show that v-Jun enables cells to express cyclin A and cyclin A-cdk2 kinase activity in the absence of growth factors and that deregulation of cdk2 is required for S-phase entry. Cyclin A expression is repressed in quiescent cells by E2F acting in conjunction with its pocket protein partners Rb, p107, and p130; however, v-Jun overrides this control, causing phosphorylated Rb and proliferation-specific E2F-p107 complexes to persist after mitogen withdrawal. Dephosphorylation of Rb and destruction of cyclin A nevertheless occur normally at mitosis, indicating that v-Jun enables cells to rephosphorylate Rb and reaccumulate cyclin A without exogenous mitogenic stimulation each time the mitotic “clock” is reset. D-cyclin–cdk activity is required for Rb phosphorylation in v-Jun-transformed cells, since ectopic expression of the cdk4- and cdk6-specific inhibitor p16 INK4A inhibits both DNA synthesis and cell proliferation. Despite this, v-Jun does not stimulate D-cyclin–cdk activity but does induce a marked deregulation of cyclin E-cdk2. In particular, hormonal activation of a conditional v-Jun–estrogen receptor fusion protein in quiescent, growth factor-deprived cells stimulates cyclin E-cdk2 activity and triggers Rb phosphorylation and DNA synthesis. Thus, v-Jun overrides the mitogen dependence of S-phase entry by deregulating Rb phosphorylation, E2F-pocket protein interactions, and ultimately cyclin A-cdk2 activity. This is the first report, however, that cyclin E-cdk2, rather than D-cyclin–cdk, is likely to be the critical Rb kinase target of v-Jun.
Peran Iklan, Brand Image, Price, Trust, dan Percieved Value Terhadap Repurchase Intention Traveloka

Penelitian ini bertujuan untuk mengetahui peran Iklan, Brand Image, Price, Trust, dan Percieved Value terhadap Repurchase Intention Traveloka , secara langsung maupun tidak langsung. Jenis penelitian ini adalah explanatory research yang menjelaskan hubungan kausal antara variabel- variabel melalui pengujian hipotesis. Penelitian ini menggunakan sampel sebanyak 400 responden. Pengambilan sampel menggunakan teknik purposive sampling yaitu tipe pemilihan sampel secara acak yang diperoleh dengan menggunakan pertimbangan tertentu, umumnya disesuaikan dengan tujuan atau masalah penelitian. Penelitian ini merupakan penelitian yang menggunakan banyak indikator dari variable eksogen, jumlah sampel yang cukup banyak. Oleh karena itu, penelitian ini menggunakan metode PLS SEM dengan SmartPLS, uji yang digunakan untuk menguji instrumen penelitian ini berupa uji pilot test, uji Outermodel, dan uji Innermodel . Uji hipotesis dilakukan menggunakan uji t, dan hipotesis mediasi menggunikan uji Sobel. K at a Kunci : Iklan, Brand Image, Price, Trust, Percieved Value, dan Repurchase Intention .
THE IMMUNOFLUORESCENCE VISUALIZATION ALR ( AUGMENTER OF LIVER REGENERATION ] = REVEALS ITS PRESENCE IN PLATELETS AND MALE GERM CELLS

* The (trSt three authoIS contributed equally to this paper. 1 University of Pittsburgh Medical center, Pittsburgh. PA .• U.S.A. 2Department of Pathology. Boston Universjty Medical Sdlool 3Depanment of Pathology, Harvard Medical Schoo), Department of Pathology, Massachusetts ,General Hospital, and Massachusetts General Hospital Cancer Center, Bldg. 149, 13th Street. Olarlestown (Boston) MA 02129 , U.S.A.
Electronically Radiation Pattern Steerable Antennas Using Active Frequency Selective Surfaces

This paper proposes a novel method of building electronically beam steerable antenna system using active frequency selective surfaces (AFSS). The design methods and special principals of the active frequency selective surfaces built for this kind of antenna are studied. The antenna system is fabricated and experimentally investigated. The front-to-back ratio is more than 30 dB with the a null point of -59 dB. The gain can be promoted to be 7.0 dBi. Furthermore, multi-beam functionality is achieved and the amplitudes of the beams can be controlled.
Travel, roads, risks and volunteers

ABSTRACT International volunteers often travel to destinations (low- and middle-income countries) that carry a greater chance of being involved in a road-related injury and a possible fatality. Within this cultural exchange, tourists move from safe roads and cars to those that are potentially unsafe. Is this all part of the adventure of volunteering or should the tourist industry take some responsibility? This paper highlights the intersection between tourism and road injury and fatality risks (as pedestrians or in vehicles) with special attention to the longer term volunteer traveller. Social science theory underpins some of the discussions around risk and travel. A brief consideration of the international road traffic death and injury crisis and the emergence of the recognition that this impacts upon tourist safety is explored as backdrop to discussions. Tourism relies upon the ability for people to travel in and around the countries they travel to, often taking on the local travel arrangements prevalent in their destination. These local travel arrangements enable leisure tourists to explore their destination and volunteer travellers to live and work as locals. Is it time to consider and further the research on road risks as a growing feature of tourism?
Elevated mutability of polA derivatives of Escherichia coli B/r at sublethal doses of ultraviolet light: evidence for an inducible error-prone repair system ("SOS repair") and its anomalous expression in these strains.

The SOS repair hypothesis proposes that UV-induced mutations in E. coli are caused by an inducible error-prone repair system (SOS repair) which is normally induced coordinately with such other recApluslexplus-dependent functions as filamentous growth, prophage induction and W-reactivation-2 in response to UV or other inhibitors of DNA synthesis. Since polA-l strains induce these functions at unusually low doses of UV, the SOS hypothesis predicts elevated UV mutability for such strains at these low doses (50 ergs per mm2 and below) Strain WP6, a polA-1 derivative of B/r, exhibits the predicted high UV mutability in this dose range, producing ten times as many Trp+ mutations as its polA+ counterpart, strain WP1, at a dose of 12.5 ergs per mm-2. The UV sensitivity of a lex polA double mutant, in which the lex and polA mutations fail to exert their individual effects on UV sensitivity additively, also confirms a prediction generated by the SOS hypothesis. --The uvrA polA-1 strain WP67 also shows elevated UV mutability at very low UV doses (5 ergs per mm-2 and below), producing about ten times as many Trp+ mutations at a dose of 0.6 ergs per mm-2 as its uvrA polA+ parent strain WP2s. It is proposed that the double mutant induces the SOS repair system (and probably other "reclex" inducible functions) at unusually low doses of UV as a consequence of its relatively inefficient repair of DNA damage when plated on broth-supplemented media.
Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses

Soluble mediators such as tumor necrosis factor alpha (TNF-alpha) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-alpha secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90% PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10% autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-micron polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-alpha activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TNF-alpha serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-alpha activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-alpha within the lungs and lead to the cachectic state associated with chronic pneumonia.
High-latitude forcing of interior ocean D 13 C

[1] Transit time distribution probability density functions (TTDs) are used to investigate the possible role of changing boundary conditions in driving the dC signal in the interior of a steady state ocean. We use idealized examples to investigate the general question of how a conservative tracer propagates from the surface ocean to interior ocean and to illustrate how a given tracer boundary signal will be ‘‘filtered’’ with increasing distance from its source region. We show that tracers in the deep southeast Atlantic Ocean will respond much more strongly to changes in the surface Southern Ocean than to changes in the high-latitude North Atlantic, while the opposite is true for waters at intermediate depths. The impact of a change in the Southern Ocean surface dC on a profile from the western South Atlantic is estimated using model-derived transit time distributions, and it is shown that significant deep ocean dC variations can be expected on glacial-interglacial timescales, even under a steady state circulation regime. Records of dC from the high-latitude North Atlantic and Southern Ocean are used as a proxy for glacial-interglacial changes in the surface ocean boundary condition in regions of deepwater formation. By convolving these high-latitude boundary conditions with model-derived TTDs, we are able to explain a significant part of the observed variability in benthic dC records spanning the last glacial cycle(s) from locations as diverse as the equatorial Atlantic Ocean, the Cape Basin, and the equatorial Pacific. This suggests that changing boundary conditions may be driving a significant fraction of benthic dC variability previously attributed to changes in ocean circulation. Furthermore, we show that our results predict a slightly higher dC than observed in high-productivity regions, consistent with the concept of a productivity-induced low-dC overprint. Citation: Rutberg, R. L. , and S. L. Peacock (2006), High-latitude forcing of interior ocean dC, Paleoceanography, 21, PA2012, doi:10.1029/2005PA001226.
Supervision: A contested space for learning and decision making

Supervision is a core activity in social work in the UK and elsewhere, widely associated with a range of positive outcomes for service users and professionals, while Public Inquiries and Serious Case Reviews have identified poor supervision as a factor in some child deaths. While there is agreement on the nature and value of ‘good’ supervision there are debates on the possibilities of delivering it in today’s pressured, poorly resourced working environments and a lack of data on what actually happens in supervision. This article presents some findings from interviews with social workers and their managers drawn from a wider study on how social workers make decisions in child protection work. They suggest that supervision is an important site for evaluating practitioner accounts and thereby constructing knowledge and making decisions about cases against a background of uncertainty and complexity. However, the ways in which these processes were negotiated shed light on supervision as a complex social process with a range of unofficial, tacit functions, embedded in the webs of social actions and exchanges that created and sustained the identities of the practitioners and their teams. What also emerged were the complex skills experienced supervisors developed in challenging and refining practitioner accounts, skills which novice supervisors struggled to acquire.
Inferring weak population structure with the assistance of sample group information

Genetic clustering algorithms require a certain amount of data to produce informative results. In the common situation that individuals are sampled at several locations, we show how sample group information can be used to achieve better results when the amount of data is limited. New models are developed for the structure program, both for the cases of admixture and no admixture. These models work by modifying the prior distribution for each individual's population assignment. The new prior distributions allow the proportion of individuals assigned to a particular cluster to vary by location. The models are tested on simulated data, and illustrated using microsatellite data from the CEPH Human Genome Diversity Panel. We demonstrate that the new models allow structure to be detected at lower levels of divergence, or with less data, than the original structure models or principal components methods, and that they are not biased towards detecting structure when it is not present. These models are implemented in a new version of structure which is freely available online at http://pritch.bsd. uchicago.edu/structure.html.
Uncommon commonalities: Cosmopolitan ethics as a framework for music education policy analysis

ABSTRACT Contemporary American education policy rhetoric is problematic because its authors’ assertions, particularly those about the goals of education, frequently conflict with their implied moral and/or ethical commitments. This philosophical policy analysis uses Appiah's cosmopolitan principles to examine the ethical implications of current education and music education policy discourse and practice. While education policy rhetoric promoting the fulfillment of basic human needs through employment aligns with one aspect of the cosmopolitan principle of universal concern, policy makers’ assertions about unbridled economic competition contradicts cosmopolitans’ concern for all individuals. Contemporary education and music education policies that enable teachers to have freedom in selecting content and instructional practices reinforce the cosmopolitan principle of respect for legitimate difference, but discourse and action emphasizing standardization, including standardized outcomes, conflicts with the cosmopolitanism. Four recommendations are offered: use cosmopolitan ethics as a means of framing agreement or dissatisfaction with contemporary education policies; implement parts of the National Core Music Standards in ways that promote standards without standardization; advocate for the aspects of the Opportunity-To-Learn Standards for Music Instruction; consider how music educators’ day-to-day practices interface with the cosmopolitan ethics.
Parents’ Experiences of Caring for a Child With Chronic Pain

Involvement of parents in their children’s pain management is universally accepted as best practice, yet there is little understanding of their needs. Twelve parents of children with chronic pain were recruited to this study in which the impact of caring for a child with chronic pain was explored. All parents started in distress, and most moved into a stance that enabled them to balance the child’s needs with their own. These parents discussed “stepping back” from their child’s distress and gaining mastery over fearful emotional reactions. A minority remained in distress, finding an adaptive response to the child’s pain challenging. Catastrophic thinking, fear of pain, and the desire to fulfill a nurturing parental role led parents to place themselves continually “on call. ” Findings indicate that parents might require support to care effectively for their children, and that many of the actions necessitated by children’s pain require complex and counterintuitive decisions.
Computed tomography-guided percutaneous biopsy of bone lesions: rate of diagnostic success and complications*

Objective To determine the rates of diagnostic success and complications of computed tomography (CT)-guided percutaneous biopsy of bone lesions suspected for malignancy. Materials and Methods Retrospective study including 186 cases of CT-guided percutaneous biopsies of bone lesions in the period from January, 2010 to December, 2012. All the specimens were obtained with 8-10 gauge needles. The following data were collected: demographics, previous history of malignancy, data related to the lesion, to the procedure, and to histological results. Results Most patients were women (57%), and the mean age was 53.0 ± 16.4 years. In 139 cases (74.6%), there was diagnostic suspicion of metastasis and the most common primary tumors were breast (32.1%) and prostate (11.8%). The bones most commonly involved were spine (36.0%), hip (32.8%) and long bones (18.3%). Complications occurred in only three cases (1.6%) including bone fracture, paresthesia with functional impairment, and needle breakage requiring surgical removal. The specimens collected from 183 lesions (98.4%) were considered appropriate for diagnosis. Malignant results were more frequently found in patients who had a suspected secondary lesion and history of known malignancy (p < 0.001), and in patients who underwent PET/CT-guided procedures (p = 0.011). Conclusion CT-guided percutaneous biopsy is a safe and effective procedure for the diagnosis of suspicious bone lesions.
Efficacy of maintenance treatment approaches for childhood overweight: a randomized controlled trial.

CONTEXT No trials for childhood overweight have examined maintenance interventions to augment the effects of initial weight loss programs. OBJECTIVES To determine the short-term and long-term efficacy of 2 distinct weight maintenance approaches vs no continued treatment control following standard family-based behavioral weight loss treatment for childhood overweight, and to examine children's social functioning as a moderator of outcome. DESIGN, SETTING, AND PARTICIPANTS A parallel-group, randomized controlled trial conducted between October 1999 and July 2004 in a university-based weight control clinic. Participants were 204 healthy 7- to 12-year-olds, 20% to 100% above median body mass index (BMI) for age and sex, with at least 1 overweight parent. Children enrolled in 5 months of weight loss treatment and 150 were randomized to 1 of 3 maintenance conditions. Follow-up assessments occurred immediately following maintenance treatments and 1 and 2 years following randomization. INTERVENTIONS Maintenance conditions included the control group or 4 months of behavioral skills maintenance (BSM) or social facilitation maintenance (SFM) treatment. MAIN OUTCOME MEASURES BMI z score and percentage overweight. RESULTS Children receiving either BSM or SFM maintained relative weight significantly better than children assigned to the control group from randomization to postweight maintenance (P< or =.01 for all; effect sizes d = 0.72-0.96; mean changes in BMI z scores = -0.04, -0.04, -0.05, and 0.05 for BSM alone, SFM alone, BSM and SFM together, and the control group, respectively). Active maintenance treatment efficacy relative to the control group declined during follow-up, but the effects of SFM alone (P = .03; d = 0.45; mean change in BMI z score = -0.24) and when analyzed together with BSM (P = .04; d = 0.38; mean change in BMI z score = -0.22) were significantly better than the control group (mean change in BMI z score = -0.06) when examining BMI z score outcomes from baseline to 2-year follow-up. Baseline child social problem scores moderated child relative weight change from baseline to 2-year follow-up, with low social problem children in SFM vs the control group having the best outcomes. CONCLUSIONS The addition of maintenance-targeted treatment improves short-term efficacy of weight loss treatment for children relative to no maintenance treatment. However, the waning of effects over follow-up, although moderated by child initial social problems, suggests the need for the bolstering of future maintenance treatments to sustain effects. TRIAL REGISTRATION clinicaltrials.gov Identifier: NCT00301197.
Recombinant Salmonella enterica Serovar Typhimurium as a Vaccine Vector for HIV-1 Gag

The HIV/AIDS epidemic remains a global health problem, especially in Sub-Saharan Africa. An effective HIV-1 vaccine is therefore badly required to mitigate this ever-expanding problem. Since HIV-1 infects its host through the mucosal surface, a vaccine for the virus needs to trigger mucosal as well as systemic immune responses. Oral, attenuated recombinant Salmonella vaccines offer this potential of delivering HIV-1 antigens to both the mucosal and systemic compartments of the immune system. So far, a number of pre-clinical studies have been performed, in which HIV-1 Gag, a highly conserved viral antigen possessing both T- and B-cell epitopes, was successfully delivered by recombinant Salmonella vaccines and, in most cases, induced HIV-specific immune responses. In this review, the potential use of Salmonella enterica serovar Typhimurium as a live vaccine vector for HIV-1 Gag is explored.
Made in Italy: Small-Scale Industrialization and Its Consequences.

Making Sense of Social Tranformation Economy and Society, 1881-1945 Social Classes and Politics, 1881-1945 The New Industrial Order, 1945-1988 The Contemporary Labor Process, 1945-1988 The New Class Structure, 1945-1988 Politics and Ideology, 1945-1988 Conclusion Selected Bibliography Index
Safety Evaluation Studies of Citrullus Colocynthis for diabetes in Rats

An experiment conducted in the department of Pharmacology and Toxicology, Nagpur Veterinary College, Nagpur reported Citrullus colocynthis to posses antidiabetic effect in rats at the rate of 50 mg/kg b.w and 100mg/kg b.w. So it was decided to also evaluate the safety of Citrullus colocynthis in rats at its antidiabetic dose. The experiment was conducted for 28 days . 18 wistar rats were divided into three groups containing six rats in each group. Group T 1 was maintained as normal control, whereas groups T2 and T3 received aqueous extract of Citrullus colocynthis @ 50 and 100 mg/kg body weight. Haematological and Biochemical estimations were done at the end of experiment i.e. on 29th day by using standard kits. Rats were then sacrificed and histopathological examinations were done. The results obtained showed that Citrullus colocynthis is safe at its antidiabetic dose and is safe for use as an antidiabetic remedy.
Connectivity vs capacity in dense ad hoc networks

We study the connectivity and capacity of finite area ad hoc wireless networks, with an increasing number of nodes (dense networks). We find that the properties of the network strongly depend on the shape of the attenuation function. For power law attenuation functions, connectivity scales, and the available rate per node is known to decrease like 1//spl radic/n. On the contrary, if the attenuation function does not have a singularity at the origin and is uniformly bounded, we obtain bounds on the percolation domain for large node densities, which show that either the network becomes disconnected, or the available rate per node decreases like 1/n.