Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial distribution and differential expression of the PBAN receptor in tissues of adult Helicoverpa spp. (Lepidoptera: Noctuidae)

Pheromone‐biosynthesis‐activating neuropeptide (PBAN) regulates sex pheromone production in many female moths. PBAN‐like peptides, with common FXPRLamide C‐terminals are found in other insect groups where they have other functions. The ubiquity and multifunctional nature of the pyrokinin/PBAN family of peptides suggests that the PBAN receptor proteins could also be present in a variety of insect tissues with alternative functions from that of sex pheromone biosynthesis. Previously we showed the presence of the PBAN‐R in Helicoverpa armigera at the protein level. In the present study we confirm the similarities between the two Helicoverpa species: armigera and zea by (1) demonstrating the presence of the receptor protein in Sf9 cells, cloned to express the HezPBAN receptor, as compared with the endogenous receptor protein, previously shown in H. armigera pheromone glands, and (2) by identifying the nucleotide sequence of the PBAN‐R from mRNA of H. armigera pheromone glands. Sequences of the two Helicoverpa spp. are 98% identical with most changes taking place in the 3′‐end. We demonstrate the spatial distribution of the PBAN receptor protein in membranes of H. armigera brain (Br), thoracic ganglion (TG) and ventral nerve cord (VNC). We also demonstrate the presence and differential expression of the PBAN receptor gene (using reverse transcription–polymerase chain reaction and reverse transcription–quantitative real‐time polymerase chain reaction, respectively) in the neural tissues (Br, TG and VNC) of adult H. armigera female moths as compared with its presence in pheromone glands. Surprisingly, the gene for the PBAN receptor is also detected in the male tissue homologous to the female pheromone gland, the aedeagus, although the protein is undetectable and PBAN does not induce physiological (pheromone production) or cellular (cyclic‐adenosine monophosphate production) responses in this tissue. Our findings indicate that PBAN or PBAN‐like receptors are present in the neural tissues and may represent a neurotransmitter‐like function for PBAN‐like peptides. In addition, the surprising discovery of the presence of the gene encoding the PBAN receptor in the male homologous tissue, but its absence at the protein level, launches opportunities for studying molecular regulation pathways and the evolution of these G protein coupled receptors (GPCRs).
Synaptic Cooperativity Regulates Persistent Network Activity in Neocortex

During behavioral quiescence, the neocortex generates spontaneous slow oscillations, which may consist of up-states and down-states. Up-states are short epochs of persistent activity that resemble the activated neocortex during arousal and cognition. Neural activity in neocortical pathways can trigger up-states, but the variables that control their occurrence are poorly understood. We used thalamocortical slices from adult mice to explore the role of thalamocortical and intracortical synaptic cooperativity (the number of coincident afferents) in driving up-states. Cooperativity was adjusted by varying the intensity of electrical or blue-light stimuli in pathways that express channelrhodopsin-2. We found that optogenetics greatly improves the study of thalamocortical pathways in slices because it produces thalamocortical responses that resemble those observed in vivo. The results indicate that more synaptic cooperativity, caused by either thalamocortical or intracortical fast AMPA-receptor excitation, leads to more robust inhibition of up-states because it drives stronger feedforward inhibition. Conversely, during strong synaptic cooperativity that suppresses up-states, blocking fast excitation, and as a result the feedforward inhibition it drives, unmasks up-states entirely mediated by slow NMDA-receptor excitation. Regardless of the pathway's origin, cooperativity mediated by fast excitation is inversely related to the ability of excitatory synaptic pathways to trigger up-states in neocortex.
The impact of reflective writing paper on non-English major learners’ autonomy in the context of Vietnam

The article aims to investigate the impact of the reflective writing paper on non- English major learners’ learning autonomy in a Vietnamese context. The data was collected by means of questionnaire, competence tests and interviews. The participants did two reflective writing papers as an intervention during the research. The research results show the possibilities for teachers to modify their teaching methods through analysis of feedback from the subjects. The findings showed that most participants appreciated the role of the reflective writing paper and they were aware of the significance of learning autonomy in their study process. Journal of NELTA, Vol. 17 No. 1-2, December 2012, Page 104-128 DOI: http://dx.doi. org/10.3126/nelta.v17i1-2.8102
Perfectionism, Coping, and Quality of Intimate Relationships

The present study examined the associations among perfectionism, marital coping, and marital functioning in a community sample of 76 couples. A theoretical model was tested in which maladaptive coping mediates the relationship between trait perfectionism and poorer marital functioning. As predicted, one of the interpersonal dimensions of perfectionism, socially prescribed perfectionism, was associated with maladaptive marital coping and poorer marital adjustment for both the self and the partner, even after controlling for depression and neuroticism. Finally, the use of negative coping strategies mediated the relationship between socially prescribed perfectionism and poorer marital functioning for both the self and the partner. Overall, this study highlights the importance of spouse-specific forms of perfectionism in marital adjustment.
Evolution of Hormone-Receptor Complexity by Molecular Exploitation

According to Darwinian theory, complexity evolves by a stepwise process of elaboration and optimization under natural selection. Biological systems composed of tightly integrated parts seem to challenge this view, because it is not obvious how any element's function can be selected for unless the partners with which it interacts are already present. Here we demonstrate how an integrated molecular system—the specific functional interaction between the steroid hormone aldosterone and its partner the mineralocorticoid receptor—evolved by a stepwise Darwinian process. Using ancestral gene resurrection, we show that, long before the hormone evolved, the receptor's affinity for aldosterone was present as a structural by-product of its partnership with chemically similar, more ancient ligands. Introducing two amino acid changes into the ancestral sequence recapitulates the evolution of present-day receptor specificity. Our results indicate that tight interactions can evolve by molecular exploitation—recruitment of an older molecule, previously constrained for a different role, into a new functional complex.
A content-driven framework for geolocating microblog users

Highly dynamic real-time microblog systems have already published petabytes of real-time human sensor data in the form of status updates. However, the lack of user adoption of geo-based features per user or per post signals that the promise of microblog services as location-based sensing systems may have only limited reach and impact. Thus, in this article, we propose and evaluate a probabilistic framework for estimating a microblog user's location based purely on the content of the user's posts. Our framework can overcome the sparsity of geo-enabled features in these services and bring augmented scope and breadth to emerging location-based personalized information services. Three of the key features of the proposed approach are: (i) its reliance purely on publicly available content; (ii) a classification component for automatically identifying words in posts with a strong local geo-scope; and (iii) a lattice-based neighborhood smoothing model for refining a user's location estimate. On average we find that the location estimates converge quickly, placing 51% of users within 100 miles of their actual location.
Periodontal Status in the United States, 1988–91: Prevalence, Extent, and Demographic Variation

This paper reports estimates of the periodontal status of US population derived from data from Phase 1 of the Third National Health and Nutrition Examination Survey conducted by the National Center for Health Statistics in collaboration with the National Institute of Dental Research from 1988 to 1991. A total of 7,447 dentate individuals 13 years of age and older, representing approximately 160.3 million civilian non-institutionalized Americans, received a periodontal assessment. Measurements of gingival bleeding, gingival recession level, periodontal pocket depth, and calculus were made by dental examiners. Assessments were made at the mesiobuccal and mid-buccal sites of all fully erupted permanent teeth present in two randomly selected quadrants, one maxillary and one mandibular. All data were weighted and standard errors calculated by special software to adjust for the effect of sample design. Although over 90% of persons 13 years of age or older had experienced some clinical loss of attachment (LA), only 15% exhibited more severe destruction (LA ≥ 5 mm). Prevalence of moderate and severe LA and gingival recession increased with age, while prevalence of pockets ≥ 4 mm or ≥ 6 mm did not. These data suggest that the increasing prevalence of LA with age is more associated with increasing prevalence of recession than with changes in the prevalence of pockets or age. The extent or number of affected sites with advanced conditions for loss of attachment, pocket depth, or recession was not large for any age group. Differences in prevalence of moderate and severe loss of attachment, moderate and deep pockets, and recession were found among gender and race-ethnicity groups. Females exhibited better periodontal health than males, and non-Hispanic whites exhibited better periodontal health than either non-Hispanic blacks or Mexican-Americans.
Recognizing Human Emotional State From Audiovisual Signals

Machine recognition of human emotional state is an important component for efficient human-computer interaction. The majority of existing works address this problem by utilizing audio signals alone, or visual information only. In this paper, we explore a systematic approach for recognition of human emotional state from audiovisual signals. The audio characteristics of emotional speech are represented by the extracted prosodic, Mel-frequency Cepstral Coefficient (MFCC), and formant frequency features. A face detection scheme based on HSV color model is used to detect the face from the background. The visual information is represented by Gabor wavelet features. We perform feature selection by using a stepwise method based on Mahalanobis distance. The selected audiovisual features are used to classify the data into their corresponding emotions. Based on a comparative study of different classification algorithms and specific characteristics of individual emotion, a novel multiclassifier scheme is proposed to boost the recognition performance. The feasibility of the proposed system is tested over a database that incorporates human subjects from different languages and cultural backgrounds. Experimental results demonstrate the effectiveness of the proposed system. The multiclassifier scheme achieves the best overall recognition rate of 82.14%.
Selective attention to threat: A test of two cognitive models of anxiety

Two experiments evaluated differential predictions from two cognitive formulations of anxiety. According to one view, attentional biases for threat reflect vulnerability to anxiety; and as threat inputs increase, high trait anxious individuals should become more vigilant, and low trait individuals more avoidant, of threat (Williams, Watts, MacLeod, & Mathews, 1988, 1997). However, according to a “cognitive-motivational” view, trait anxiety influences the appraisal of stimulus threat value, rather than the direction of attentional bias, and both high and low trait anxious individuals should exhibit greater vigilance for high rather than mild threat stimuli (Mogg & Bradley, 1998). To test these predictions, two experiments examined the effect of manipulating stimulus threat value on the direction of attentional bias. The stimuli included high threat and mild threat pictorial scenes presented in a probe detection task. Results from both studies indicated a significant main effect of stimulus threat value on attentional bias, as there was increased vigilance or reduced avoidance of threat, as threat value increased. This effect was found even within low trait anxious individuals, consistent with the “cognitive-motivational” view. Theoretical and clinical implications are discussed.
Studies in a Tumor Spectrum III . The Effect of Phosphor amides on the Growth of a Variety of Mouse and Rat Tumors * KANEMATSUSUGIURA NDC

Previous investigations with a spectrum of tu mors demonstrated that nitrogen mustards (17) and triethylene melamine (19) had a definite in hibitory or destructive action on certain types of tumors. In view of the fact that the biological ac tivity of these compounds is believed to be due to the formation of ethylenimmonium ions in the case of the former (7) and the presence of ethylenimine groups in the case of the latter, the study was extended with compounds closely related to these substances on a wide spectrum of tumors. Results in experimental animal tumors reported from other laboratories (1-3, 6, 9-11, Â£0, 21) and in human cancer (4, 8, 20) have indicated that tri ethylene phosphoramide and triethylene thiophosphoramide merited intensive study. Our observa tions on the effect of these compounds on a variety of transplantable mouse and rat tumors have been briefly reported (14, 18) and are now presented in more detail. The structural formulae of these com pounds have in common the cyclic structure, ethylenimine :
Case study as a research strategy: Some ambiguities and opportunities

From a general methodological point of view the status of the case study as a type of research is not quite clear. There are some ambiguities as to the object and the way we look at it, the research methods that are used, and the results to be obtained. One piece of background of its unclear status is that the case study is mostly defined as the study of one single case, rather than as a way of doing the research. Another one is that, in the eyes of many quantitative social science researchers, the results of a case study are of poor quality. This article aims to clarify some misconceptions and ambiguities of the case study as it is used in modern social science disciplines, and to define it as a research strategy of its own, clearly distinguished from and complementary to the quantitative or large scale survey type of research, the experiment, and (other) qualitative research strategies.
Urban/rural and gender differences among Canadian emerging adults

Although cultural and subcultural differences during the transition to adulthood have been examined, important factors like rural/urban upbringing and gender differences among Canadian emerging adults have been neglected. The present study explored developmentally significant tasks including criteria for adulthood, beliefs about religiosity, and risk-taking behaviors among 287 male and female Canadian emerging adults from rural and urban backgrounds. Results revealed that compared to their urban counterparts, rural emerging adults were more likely to place importance on role and biological transitions as criteria for achieving adulthood, and engaged in more risk-taking behaviors (excluding smoking). Female emerging adults were more likely to believe in the importance of role transition, norm compliance, and family capacities compared to males, and were more likely to smoke. In contrast, males were more likely than females to espouse the importance of biological transitions and engage in non-smoking risk behaviors. These findings were interpreted in light of sociocultural and gender socialization differences among emerging adults from rural and urban upbringings.
The prevalence of hypokalemia in hospitalized patients with infectious diseases problem at Cipto Mangunkusumo Hospital, Jakarta.

AIM to know the prevalence of hypokalemia that occurs in hospitalized patients with infectious diseases. METHODS a cross sectional study was carried out in the internal ward Cipto Mangunkusumo General Hospital in Jakarta from December 2005 until June 2006. All hospitalized patients with infectious diseases receiving "replacement solution" were included in this study. We collected the blood sample to perform the serum potassium level at the time of admission and discharge. RESULTS one hundred and five patients were enrolled in this study; consisting of 44 males and 61 females. The age ranged from 14 to 70 years old. The most common infectious diseases were dengue fever, while the underlying diseases were hepatobiliary disorders. "Replacement solutions" which were given, were ringer's lactate 91%, normal saline 8%, and ringer's acetate 1%. Prevalence of hypokalemia among the hospitalized patients, on admission was 24 patients (23%) and during hospitalization was 39 patients (37%). The mean level of hypokalemia on admission was 3.11 + SD 0.37 mEq/L (range from 1.7 to 3.4 mEq/L) and during hospitalization was 3.13 + SD 0.25 mEq/L (range from 2.5 to 3.4 mEq/L). On admission, the ratio of mild : moderate : severe hypokalemia is 22 : 2 : 1. And at discharge the ratio of mild to moderate hypokalemia becomes 19 : 6. CONCLUSION the prevalence of hypokalemia in hospitalized patients with infectious disease in Cipto Mangkunkusumo's Hospital, Jakarta is 23%. Further studies are needed to know the contributing factors including the usage of 'intravenous fluid' in relations to hypokalemic state during hospitalization.
About being pure and natural: understandings of pre‐service primary teachers

In an investigation of 149 pre‐service primary teachers’ understanding of the terms pure and natural, the participants were asked to provide definitions of the two words, and classify various substances including drugs of abuse, pharmaceuticals, nutriceuticals, and household substances as either natural or not natural, and as pure or not pure. A common scale of purity and naturalness could be constructed consistent with the Rasch model, suggesting that the participants conflate the two terms. This conflation is consistent with the qualitative data that suggested participants associated both concepts within a common spiritual metaphor, heavily laden with emotive content. Purity was not seen as relating to the composition of a substance, but to its history. The findings may help explain the development of persistent non‐scientific chemical taxonomies observed in secondary students. Furthermore, as health educators, the participants may become the source of inappropriate messages about the use of medicines, or the abuse of drugs.
Tears Falling on Goosebumps: Co-occurrence of Emotional Lacrimation and Emotional Piloerection Indicates a Psychophysiological Climax in Emotional Arousal

This psychophysiological study is the first to examine the relationship between emotional tears and emotional piloerection (i.e., goosebumps). Although both phenomena have been related to peak states of being moved, details about their temporal occurrence and the associated levels of physiological arousal have remained unknown. In our study, we used emotionally powerful film scenes that were self-selected by participants. Our findings show that even within peak moments of emotional arousal, a gradation of intensity is possible. The overlap of tears and goosebumps signifies a maximal climax within peak moments. On the side of the stimulus, we found that displays of prosocial behavior play a crucial role in the elicitation of tears and goosebumps. Finally, based on the results of a formal film analysis of the tears-eliciting clips provided by our participants, as compared to randomly extracted, equally long control clips from the same films, we show how the technical and artistic making of the clips was optimized for the display of social interaction and emotional expressions.
Does Relationship and Marriage Education for Lower-Income Couples Work? A Meta-Analytic Study of Emerging Research

While a large number of studies have documented how couple education programs can strengthen couple relationships, few studies have tested these programs on lower-income, higher-risk couples. Yet over the past decade, state and federal governments have been experimenting with supporting couple education programs designed to help lower-income couples form and sustain healthy marriages and relationships. This article reports the findings of a meta-analytic study of the effectiveness of couple education targeted to lower-income couples. We examined evaluation data from 15 programs and found small-to-moderate effects. For the three control-group studies, we found the overall effect of these programs was d = . 250 (p < .05). For the 12 one-group/pre-post studies, we found the overall effect was d = . 293 (p < .001). These effects are relatively similar to those found for MRE studies with middle-income participants and for studies of other family-support educational programs. Implications for couple educators are discussed.
Mucor fragilis as a novel source of the key pharmaceutical agents podophyllotoxin and kaempferol

Abstract Context: Podophyllotoxin, a pharmaceutically important bioactive compound of Podophyllum sps. (Berberidaceae), is in great demand worldwide as an anticancer and antivirus drug precursor. However, the source of podophyllotoxin is very limited due to the endangered status of the Podophyllum plant. Objective: The aim of this study was to isolate podophyllotoxin-producing endophytic fungi from Sinopodophyllum hexandrum (Royle) Ying (1979) (Berberidaceae) plants of the Taibai Mountains of China in order to obtain bioactive compounds. Materials and methods: The strains producing kaempferol and podophyllotoxin were screened by thin-layer chromatography (TLC) analysis. The presence of kaempferol and podophyllotoxin in extracts of these strains was further confirmed by high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) analyses. Results: Among six endophytic fungi isolated from the rhizomes of S. hexandrum, one strain was able to produce kaempferol. Another strain, named TW5, was able to produce both kaempferol and podophyllotoxin simultaneously according to the TLC, HPLC, and NMR results. The podophyllotoxin yield of TW5 was calculated to be 49.3 μg/g of mycelial dry weight after 7-d fermentation. Strain TW5 was identified morphologically and phylogenetically to be Mucor fragilis Fresen. (Mucoraceae). These results suggest that the podophyllotoxin-synthesizing ability is obtained by uptaking genes involved in the podophyllotoxin synthesis from the host plant into endophytic fungal genomes. Conclusion: Our results showed, for the first time, that the endophytic fungus M. fragilis is able to produce simultaneously the same two bioactive metabolites, podophyllotoxin and kaempferol, as its host plant. Furthermore, the relatively high podophyllotoxin yield obtained may improve the industrial production of podophyllotoxin, which may help protect this endangered plant.