Miracle-dz/UniFAR-FTUs huggingface dataset:
Diversity of surface protein expression in group B streptococcal colonizing & invasive isolates.

BACKGROUND & OBJECTIVES The classification of group B streptococcal (GBS) isolates is based on the capsular polysaccharides (Ia-VIII), and antigenic characterization of clinical isolates is augmented by the detection of various surface-localized protein antigens. In our laboratory, all GBS isolates are routinely analysed for the alpha trypsin-resistant and the beta trypsin-sensitive c protein antigens, as well as other trypsin-resistant proteins R1, R3, and R4, as well as BPS. The purpose of this work was to study diversity of protein expression in colonizing isolates (vaginal and rectal sites) from nonpregnant women and from invasive isolates (blood or CSF) from mothers and their less than seven day old newborn infants. METHODS A total of 289 invasive isolates and 2660 colonizing isolates were collected between 1993-2002. All isolates were tested for polysaccharide serotype and cell surface-expressed protein profile by double immunoprecipation in agarose using monospecific antisera. RESULTS Among the 289 invasive isolates, 89.6 per cent expressed one or more trypsin-resistant proteins; 93 per cent of the colonizing isolates expressed one or more of these proteins. Overall, the most common surface protein expression profile by GBS serotype was: alpha in type Ia; alpha plus beta in type Ib; alpha and R4 in type II; R4 in type III; and co-expression of R1 plus R4 in isolates of type V. BPS was found in five (1.7%) invasive isolates, alone in two isolates and with other proteins in three isolates. Among 2660 colonizing isolates, BPS was found alone in 15 (0.6%) and in 57 additional isolates with other proteins. Among the total isolates, BPS was found predominantly in serotype Ia isolates, also expressing R1. Uncommon protein profiles of known serotypes included 11 type III isolates expressing alpha plus beta. Among 72 nontypable colonizing isolates, expression of R1 plus R4 was the commonest (33.3%) profile. INTERPRETATION & CONCLUSION The GBS surface proteins and the common serotypes were distributed comparably in colonizing and invasive isolates. Trypsin-resistant, alpha and alpha-like proteins, R1 and R4 were the most prevalent. The phenotypic diversity of the surface-localized protein antigens of GBS is intriguing, and genotypic analysis will permit consensus in nomenclature from laboratory to laboratory.
CD137-CD137 Ligand Interactions in Inflammation

The main stream of CD137 studies has been directed to the function of CD137 in CD8+ T-cell immunity, including its anti-tumor activity, and paradoxically the immunosuppressive activity of CD137, which proves to be of a great therapeutic potential for animal models of a variety of autoimmune and inflammatory diseases. Recent studies, however, add complexes to the biology of CD137. Accumulating is evidence supporting that there exists a bidirectional signal transduction pathway for the CD137 receptor and its ligand (CD137L). CD137/CD137L interactions are involved in the network of hematopoietic and nonhematopoietic cells in addition to the well characterized antigen-presenting cell-T cell interactions. Signaling through CD137L plays a critical role in the differentiation of myeloid cells and their cellular activities, suggesting that CD137L signals trigger and sustain inflammation. The overall consequence might be that the amplified inflammation by CD137L enhances the T-cell activity together with CD137 signals by upregulating costimulatory molecules, MHC molecules, cell adhesion molecules, cytokines, and chemokines. Solving this outstanding issue is urgent and will have an important clinical implication.
A comparative population‐based analysis of sinonasal diffuse large B‐cell and extranodal NK/T‐cell lymphomas

Diffuse large B‐cell lymphoma (DLBCL) and extranodal natural killer/T‐cell lymphoma (ENKTL) are aggressive tumors. ENTKL is very rare in the United States and often affects the nasal cavity and paranasal sinuses; DLBCL, although more common, rarely occurs in these locations. Our study aims to compare incidence and survival of these lymphomas in the sinonasal cavity.
A Social Model of Moral Dumbfounding: Implications for Studying Moral Reasoning and Moral Judgment

Moral psychologists have recently turned their attention to a phenomenon they call ‘moral dumbfounding’. Moral dumbfounding occurs when someone confidently pronounces a moral judgment, then finds that he or she has little or nothing to say in defense of it. This paper addresses recent attempts by Jonathan Haidt and Marc Hauser to make sense of moral dumbfounding in terms of their respective theories of moral judgment; Haidt in terms of a ‘social intuitionist’ model of moral judgment, and Hauser in terms of his ‘Rawlsian creature’ model of moral judgment. I show that Haidt and Hauser assume that moral dumbfounding is to be explained in terms of features of a. ) moral judgment that are b. ) construed in terms of the intrinsic features of individuals. By contrast, I hypothesize that moral dumbfounding is to be explained in terms of moral reasoning, more specifically in terms of the social dynamics of such reasoning. Finally, I argue that this hypothesis points towards an externalistic account of both moral reasoning and moral judgment.
RAPID DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN PERIPHERAL BLOOD LEUKOCYTES OF VIREMIC TRANSPLANT RECIPIENTS BY THE POLYMERASE CHAIN REACTION

Peripheral blood leukocyte samples (n=458) of 24 bone marrow transplant and 52 kidney transplant patients were examined weekly for the presence of human cytomegalovirus (HCMV) using an improved culture technique (DEAFF; detection of early antigen fluorescent foci). In total 5 (21%) bone marrow transplant and 11 (21%) kidney transplant patients developed a viremia. Patients' samples were investigated for the presence of HCMV DNA using an in vitro DNA amplification technique, the polymerase chain reaction (PCR). From the statistically evaluable viremic patients (n=13), 110 blood samples were analyzed. In 5 of these patients, the DEAFF and PCR led to identical results. In 8 patients however the PCR was more sensitive, i.e. HCMV DNA was detected for a longer period of time. Applying statistical analysis using the McNemar test, this result was significant (P<0.05). The PCR applied on leukocyte samples did not detect HCMV DNA in viruric patients without viremia. Moreover, the current PCR never led to positive results with peripheral blood leukocyte samples of healthy seropositive or seronegative controls. Since the PCR can be performed in 6 hr, this technique will contribute to rapid detection of HCMV DNA in peripheral blood leukocytes and therefore to optimal clinical management of HCMV-infected transplant recipients.
Familial Intracranial Aneurysms: An Analysis of 346 Multiplex Finnish Families

Background and Purpose— Genetic risk factors are considered important in the development, growth, and rupture of intracranial aneurysms; however, few have been identified. We analyzed intracranial aneurysm families with at least 2 affected persons and determined relationships between affected persons and assessed the inheritance patterns of aneurysms. Methods— Families with ≥2 members with verified diagnoses of intracranial aneurysms were recruited from Kuopio and Helsinki, Finland. Families with a diagnosis of other heritable disorders that have associated intracranial aneurysms, such as autosomal dominant polycystic kidney disease, were excluded. Results— We identified 346 Finnish multiplex families with 160 (46.2%) male and 186 (53.8%) female index cases. There were a total of 937 aneurysm cases, with an average of 2.7 cases per family. The majority of the families had only 2 affected relatives (n=206; 59.5%), although there were families with up to 6 (n=10), 7 (n=1), 8 (n=1), or 10 (n=2) affected persons. The affected relatives of the index cases included 108 sisters, 116 brothers, 105 parents, 30 children, 15 grandparents, 102 aunts or uncles, and 64 cousins. Of the 937 affected persons, 569 (60.7%) were alive and available for genetic analysis. Inheritance patterns consistent with autosomal recessiveness were observed in 198 (57.2%), autosomal dominance in 126 (36.4%), and autosomal dominance with incomplete penetrance in 19 (5.5%) of the families. Conclusions— The collection is the most extensive published to date and extends previous observations of familial aggregation that are consistent with a major gene effect.
Frontline rituximab, cyclophosphamide, doxorubicin, and prednisone with bortezomib (VR-CAP) or vincristine (R-CHOP) for non-GCB DLBCL.

This phase 2 study evaluated whether substituting bortezomib for vincristine in frontline rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) therapy could improve efficacy in non-germinal center B-cell-like diffuse large B-cell lymphoma (non-GCB DLBCL), centrally confirmed by immunohistochemistry (Hans method). In total, 164 patients were randomized 1:1 to receive six 21-day cycles of rituximab 375 mg/m(2), cyclophosphamide 750 mg/m(2), and doxorubicin 50 mg/m(2), all IV day 1, prednisone 100 mg/m(2) orally days 1-5, plus either bortezomib 1.3 mg/m(2) IV days 1, 4, 8, 11 (rituximab, cyclophosphamide, doxorubicin, and prednisone with bortezomib [VR-CAP]; n = 84) or vincristine 1.4 mg/m(2) (maximum 2 mg) IV day 1 (R-CHOP; n = 80). There were no significant differences between VR-CAP and R-CHOP in complete response rate (64.5%, 66.2%; odds ratio [OR], 0.91; P = .80), overall response rate (93.4%, 98.6%; OR, 0.21; P = .11), progression-free survival (hazard ratio [HR], 1.12; P = .76), or overall survival (HR, 0.89; P = .75). Rates of grade ≥3 adverse events (AEs; 88%, 89%), serious AEs (38%, 34%), discontinuations due to AEs (7%, 3%), and deaths due to AEs (2%, 5%) were similar with VR-CAP and R-CHOP. Grade ≥3 peripheral neuropathy rates were 6% and 3%, respectively. VR-CAP did not improve efficacy vs R-CHOP in non-GCB DLBCL. This trial was registered at www.clinicaltrials. gov as #NCT01040871.
The Role of Quality of Care in Health-related Quality of Life in Patients with IBD

In the literature there are indications of associations between health-related quality of life (HRQoL) in inflammatory bowel disease and disease activity, psychological status, coping, stressful life events, and social support. The aim of this study was to examine whether a relation exists between quality of health care and HRQoL, taking possible confounding variables into account.For this purpose, one single questionnaire was compiled from existing validated questionnaires. A population-based inception cohort of 1056 patients with inflammatory bowel disease in eight countries, diagnosed 6 to 8 years prior to the study, was approached to participate.In total, 824 patients responded (78%), and 517 could be included in statistical analyses. It was shown that in inflammatory bowel disease HRQoL was indeed influenced by quality of care (particularly with regard to the parameters of “providing information,” “costs,” and “courtesy”), as well as by disease activity, psychological status, type of hospital, social support, stressful life events, and way of administration of the questionnaire. Patients with active disease had lower psychological status and HRQoL scores at the time of the survey than patients without active disease. However, quality of care scores did not differ between these groups. The care aspect “costs” was scored worse by CD compared with UC patients, probably caused by a potentially more expensive treatment.In conclusion, it is shown in a large exploratory study, for the first time, that in inflammatory bowel disease, quality of care has a significant role in determining health-related quality of life.
The prevalence of benign and malignant neoplasms in acromegalic patients.

INTRODUCTION In acromegalic patients, the prevalence of certain benign and malignant neoplasms is higher than that in the healthy population. We retrospectively evaluated the prevalence of tumours in acromegalic patients treated at our department: the regional centre for acromegalic patients for the Małopolskie voivodeship in Poland. MATERIAL AND METHODS During the years 1983-2008, a hundred and one acromegalic patients (30 males and 71 women), of mean age 51.8 +/- 15.4 years, were diagnosed and treated. Pituitary macroadenoma and microadenoma were stated in 63.4% and 25.7% of these patients, respectively. In 10.9% of these patients no data on tumour diameter were available. The mean observation period was 9.4 +/- 6.5 years. The median levels of hGH and IGF-1 prior to neurosurgery were 20.2 (IQR = 34.9) ng/ml and 764.5 (IQR = 569.6) ng/ml, respectively. RESULTS In the studied group of patients, we found the following prevalence of various tumours: nodular goitre - 64/101 patients (63.0%), polyps of the colon - 13/101 patients (13.0%); uterine polyps - 4/101 patients (4.0%); and prostate adenoma - 2/101 patients (2.0%). Among malignant tumours, thyroid cancer, endometrium and cervix cancer were the most frequent, each of these occurring in 3 patients (3.0%). Colon cancer prevalence was 2.0% (in 2 patients). CONCLUSIONS From our retrospective study, we suggest an overall increase of tumour incidence in acromegalic patients. Prospective multicentre studies are required to resolve the significance of this observation. In our study group, the number of malignant neoplasms was significantly higher in patients with long-lasting uncontrolled disease (over 5 years), compared to patients with controlled disease. (Pol J Endocrinol 2010; 61 (1): 29-34).
Input and output in code switching: a case study of a Japanese–Chinese bilingual infant

Abstract Code switching (CS) (or language mixing) generally takes place in bilingual children's utterances, even if their parents adhere to the ‘one parent – one language’ principle. The present case study of a Japanese–Chinese bilingual infant provides both quantitative and qualitative analyses on the impact of input on output, as manifested in CS. The database consists of the transcription of 12 hours of audio recordings, selected out of nearly 130 hours of recordings of an infant from the age of 2;1 to 3;0. The quantitative analysis exhibits some influence of parental input on the subject's output in regard to CS. In Qualitative Analysis A, the parental discourse strategy toward the child's CS does account for the overall high ratio of output CS. However, the parental adjustment of input strategies contradicts the present data of output. Qualitative Analysis B concerns the notion of language dominance in terms of intra-sentential CS. Our findings show that, in spite of the absolute Chinese dominance evident in the parental input, the child maintains a strong Japanese-dominant output. Hence, as far as CS is concerned, this study obtains little evidence of qualitative influence of parental input on the child's output. Instead of parental input, language dominance can better account for the increased trend of the child's output CS.
Developing oneself as a leader

Purpose – The purpose of this paper is to clarify the concept of self as applied to leadership and propose an understanding of how a leader should form conceptions of self, and use these in his or her own development.Design/methodology/approach – Based on self‐, personality‐ and developmental psychology, the paper examines a variety of theoretical foundations, and ties these into the context of leadership and self‐development.Findings – The paper concludes that the self is core, consciousness, and action. The particular characteristics and qualities of the self determine the leader's comprehension of him or herself as a human entity, and is a leader's gateway to self‐confidence and self‐esteem. Leaders therefore need to cultivate an understanding of self by engaging in formative processes which are related to their ability to learn from defining situations, thus raising awareness of points of convergence in a leader's career.Research limitations/implications – The paper is limited to a conceptual discussi.
A histological classification of carcinomas of the nasopharynx with a critical review as to the existence of lymphoepitheliomas

ESPITE MANY excellent papers on nasoD pharyngeal carcinomas, a generally acceptable classification of these tumors is lacking. The more papers one examines, the more difficulty one has in understanding what is meant by the terms that the various writers have employed. For instance, under carcinoma, one finds the following types mentioned: squamous carcinoma, scirrhous carcinoma, epidennoid carcinoma, nonepidermoid carcinoma, intermediary carcinoma, epidermoid anaplastic caranoma, undifferentiated carcinoma, transitional cell carcinoma, lymphoepi thelioma, basal cell carcinoma, and adenocarcinoma. Although it is well known that most carcinomas of the nasopharynx are undiffereniated, each author engaged in studying this tumor constructs his own more detailed classification. Perhaps this is because of the rarity of nasopharyngeal cancer in the Western world. Even in large medical centers, not more than a hundred of these cancers are seen in a period of from 10 to 20 years. Therefore, any classification based on these small series can represent only a few facets of this complicated problem. Presently, with the steady increase in case reports, and after hot discussions by various authors, the main problem seems to be centered on transitional cell carcinomas and IymphoepitheIiomas. According to many authors,7* s, l3 both tumors consist of the same component cells and both show high radiosensitivity, the only difference being that lymphocytes are intimately mixed with malignant cells in lymphoepitheliomas but almost lacking in transitional cell carcinomas. The main aim of this study was to construct a purely histological classification of carcinoma of the nasopharynx by using a large
Tanshinone IIA attenuates interleukin-17A-induced systemic sclerosis patient-derived dermal vascular smooth muscle cell activation via inhibition of the extracellular signal-regulated kinase signaling pathway

OBJECTIVE: Salvia miltiorrhiza has long been used to treat systemic sclerosis. Tanshinone IIA, one of the phytochemicals derived from the roots of Salvia miltiorrhiza, exhibits multiple biological activities. The present study aimed to investigate whether tanshinone IIA has an effect on the interleukin-17A-induced functional activation of systemic sclerosis patient-derived dermal vascular smooth muscle cells. METHODS: Systemic sclerosis patient-derived dermal vascular smooth muscle cells were incubated with various dosages of tanshinone IIA in the presence of interleukin-17A or the serum of systemic sclerosis patients. Cell proliferation was assessed using Cell Counting Kit-8. The expression of collagen 1 and 3 in cells was evaluated by immunofluorescence. Cell migration was measured using a transwell assay. The expression of phospho-extracellular signal-regulated kinase was detected by Western blotting. RESULTS: Our data demonstrate that tanshinone IIA exerts an inhibitory effect on interleukin-17A-induced systemic sclerosis patient-derived dermal vascular smooth muscle cell proliferation, collagen synthesis and migration. CONCLUSION: These findings suggest that tanshinone IIA might serve as a promising therapeutic agent for the treatment of systemic sclerosis.
Discourses on sexual pleasure after genital modifications: the fallacy of genital determinism (a response to J. Steven Svoboda)

This is a reply to:Svoboda, J. Steven 2013. “Promoting genital autonomy by exploring commonalities between male, female, intersex, and cosmetic female genital cutting. ” Global Discourse. 3 (2): 237–255. http://dx.doi. org/10.1080/23269995.2013. 804757
CGI-58/ABHD5-Derived Signaling Lipids Regulate Systemic Inflammation and Insulin Action

Mutations of comparative gene identification 58 (CGI-58) in humans cause Chanarin-Dorfman syndrome, a rare autosomal recessive disease in which excess triacylglycerol (TAG) accumulates in multiple tissues. CGI-58 recently has been ascribed two distinct biochemical activities, including coactivation of adipose triglyceride lipase and acylation of lysophosphatidic acid (LPA). It is noteworthy that both the substrate (LPA) and the product (phosphatidic acid) of the LPA acyltransferase reaction are well-known signaling lipids. Therefore, we hypothesized that CGI-58 is involved in generating lipid mediators that regulate TAG metabolism and insulin sensitivity. Here, we show that CGI-58 is required for the generation of signaling lipids in response to inflammatory stimuli and that lipid second messengers generated by CGI-58 play a critical role in maintaining the balance between inflammation and insulin action. Furthermore, we show that CGI-58 is necessary for maximal TH1 cytokine signaling in the liver. This novel role for CGI-58 in cytokine signaling may explain why diminished CGI-58 expression causes severe hepatic lipid accumulation yet paradoxically improves hepatic insulin action. Collectively, these findings establish that CGI-58 provides a novel source of signaling lipids. These findings contribute insight into the basic mechanisms linking TH1 cytokine signaling to nutrient metabolism.
What's Wrong with Chinese Journalists? Addressing Journalistic Ethics in China Through a Case Study of the Beijing Youth Daily

This paper uses Beijing Youth Daily, the second biggest local newspaper in Beijing, as a case study to examine Chinese news people's perceptions of their professional roles and unethical practices. The author argues that Chinese journalistic professionalism has developed. Journalists see their most fundamental role as that of disseminator. Their concepts of professional roles and virtues are surprisingly similar to those held by journalists in liberal democratic countries. However, Chinese journalists' partial representation of the party/state and their tolerance towards unethical practices such as paid journalism or “red envelopes” suggest they are more likely to be under pressures from both politics and commerce.
Fixed Penalty Notices as a Means of Offender Selection

This research assesses the capacity of fixed penalty notice (FPN) infractions to form the basis for targeted police attention to more serious or chronic offenders. Offences of this kind were associated with concurrent criminality, relative to a group selected from the electoral register. Over half of the notices issued were not associated with a named individual, presumably because many vehicles did not have a current registered keeper. The ‘hit rate’ for concurrent criminality was not significantly different than for the control group. When repeat fixed penalty offences were analysed, this produced a higher hit rate, although it remained lower than expected from other studies. A number of problems in carrying out analysis on these types of data are discussed and it is suggested that a study in which checks are made at the point of issuing an FPN might well yield far higher rates of active criminality, and might provide a more powerful trigger for offender self-selection. Further areas for research into self-selection triggers are also suggested.