Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of oakmoss and its components on Acanthamoeba castellanii ATCC 30234 and the uptake of Legionella pneumophila JCM 7571 (ATCC 33152) into A. castellanii.

Acanthamoeba castellanii, a ubiquitous organism in water environments, is pathogenic toward humans and also is a host for bacteria of the genus Legionella, a causative agent of legionellosis. Oakmoss, a natural fragrance ingredient, and its components are antibacterial agents specifically against the genus Legionella. In the present study, oakmoss and its components were investigated for their amoebicidal activity against A. castellanii ATCC 30234 and the inhibitory effect on the uptake of L. pneumophila JCM 7571 (ATCC 33152) into A. castellanii. The oakmoss and its components 3-hydroxy-5-methylphenyl 2,4-dihydroxy-6-methylbenzoate(5), and 6,8-dihydroxy-3-pentyl-1H-isochromen-1-one (12) exhibited high amoebicidal activity (IC50 values; 10.5 ± 2.3, 16.3 ± 4.0 and 17.5 ± 2.8 μg/mL, respectively) after 48 h of treatment, which were equivalent to that of the reference compound, chlorhexidine gluconate. Pretreatment of L. pneumophila with sub-minimal inhibitory concentration of oakmoss, compound 5, 3-hydroxy-5-methylphenyl 2-hydroxy-4-methoxy-6-methylbenzoate (10) and 8-(2,4-dihydroxy-6-pentylphenoxy)-6-hydroxy-3-pentyl-1H-isochromen-1-one (14) obviously reduced the uptake of L. pneumophila into A.castellanii (p < 0.05). The inhibitory effect of compound 5 on the uptake of L. pneumophila was almost equivalent to that of ampicillin used as a reference. Thus, the oakmoss and its components were considered to be good candidates for disinfectants against not only genus Legionella but also A. castellanii.
Initiation of HIV Reverse Transcription

Reverse transcription of retroviral genomes into double stranded DNA is a key event for viral replication. The very first stage of HIV reverse transcription, the initiation step, involves viral and cellular partners that are selectively packaged into the viral particle, leading to an RNA/protein complex with very specific structural and functional features, some of which being, in the case of HIV-1, linked to particular isolates. Recent understanding of the tight spatio-temporal regulation of reverse transcription and its importance for viral infectivity further points toward reverse transcription and potentially its initiation step as an important drug target.
Developing and Validating Band Levels and Descriptors for Reporting Overall Examinee Performance

This study presents the development and empirical validation of score levels and descriptors specifically designed for reporting purposes to provide test takers with more than just a number on a score scale. In the context of a test primarily intended for 11- to 15-year-old students learning English as a second/foreign language, the study examined the number of band levels that could be meaningfully distinguished, the reliability of the classification of students into these band levels, and the development of overall performance descriptors that would provide meaningful information to score users. The performance data from 2,931 students who took the test were used. The band level solution was determined by balancing considerations for the reliability of classification decisions and the desire for the levels to represent meaningful performance differences. To construct meaningful descriptors for the band levels, multiple sources of information were examined, including the scoring rubrics, the characteristics of test items, typical student performance profiles, and the performance of norm groups on the test. The importance of establishing the psychometric quality of band levels and the empirical basis for performance descriptors, as well as the implications for similar efforts, are discussed.
Splenic pooling and loss of VCAM-1 causes an engraftment defect in patients with myelofibrosis after allogeneic hematopoietic stem cell transplantation

Myelofibrosis is a myeloproliferative neoplasm that results in cytopenia, bone marrow fibrosis and extramedullary hematopoiesis. Allogeneic hematopoietic stem cell transplantation is the only curative treatment but is associated with a risk of delayed engraftment and graft failure. In this study, patients with myelofibrosis (n=31) and acute myeloid leukemia (n=31) were analyzed for time to engraftment, graft failure and engraftment-related factors. Early and late neutrophil engraftment and late thrombocyte engraftment were significantly delayed in patients with myelofibrosis as compared to acute myeloid leukemia, and graft failure only occurred in myelofibrosis (6%). Only spleen size had a significant influence on engraftment efficiency in myelofibrosis patients. To analyze the cause for the engraftment defect, clearance of hematopoietic stem cells from peripheral blood was measured and immunohistological staining of bone marrow sections was performed. Numbers of circulating CD34+ were significantly reduced at early time points in myelofibrosis patients, whereas CD34+CD38− and colony-forming cells showed no significant difference in clearance. Staining of bone marrow sections for homing proteins revealed a loss of VCAM-1 in myelofibrosis with a corresponding significant increase in the level of soluble VCAM-1 within the peripheral blood. In conclusion, our data suggest that reduced engraftment and graft failure in myelofibrosis patients is caused by an early pooling of CD34+ hematopoietic stem cells in the spleen and a bone marrow homing defect caused by the loss of VCAM-1. Improved engraftment in myelofibrosis might be achieved by approaches that reduce spleen size and cleavage of VCAM-1 in these patients prior to hematopoietic stem cell transplantation.
Effective persistent homology of digital images

In this paper, three Computational Topology methods (namely effective homology, persistent homology and discrete vector fields) are mixed together to produce algorithms for homological digital image processing. The algorithms have been implemented as extensions of the Kenzo system and have shown a good performance when applied on some actual images extracted from a public dataset.
Gender contributes to personal research funding success in The Netherlands

Significance Women remain underrepresented in academia as they continue to face a leadership gap, salary gap, and funding gap. Closing the funding gap is of particular importance, because this may directly retain women in academia and foster the closing of other gaps. In this study, we examined the grant funding rates of a national full population of early career scientists. Our results reveal gender bias favoring male applicants over female applicants in the prioritization of their “quality of researcher” (but not “quality of proposal”) evaluations and success rates, as well as in the language use in instructional and evaluation materials. This work illuminates how and when the funding gap and the subsequent underrepresentation of women in academia are perpetuated. We examined the application and review materials of three calls (n = 2,823) of a prestigious grant for personal research funding in a national full population of early career scientists awarded by the Netherlands Organization for Scientific Research (NWO). Results showed evidence of gender bias in application evaluations and success rates, as well as in language use in instructions and evaluation sheets. Male applicants received significantly more competitive “quality of researcher” evaluations (but not “quality of proposal” evaluations) and had significantly higher application success rates than female applicants. Gender disparities were most prevalent in scientific disciplines with the highest number of applications and with equal gender distribution among the applicants (i.e., life sciences and social sciences). Moreover, content analyses of the instructional and evaluation materials revealed the use of gendered language favoring male applicants. Overall, our data reveal a 4% “loss” of women during the grant review procedure, and illustrate the perpetuation of the funding gap, which contributes to the underrepresentation of women in academia.
On estimating attenuation from the amplitude of the spectrally whitened ambient seismic field

SUMMARY Measuring attenuation on the basis of interferometric, receiver–receiver surface waves is a non-trivial task: the amplitude, more than the phase, of ensemble-averaged cross-correlations is strongly affected by non-uniformities in the ambient wavefield. In addition, ambient noise data are typically pre-processed in ways that affect the amplitude itself. Some authors have recently attempted to measure attenuation in receiver–receiver cross-correlations obtained after the usual pre-processing of seismic ambient-noise records, including, most notably, spectral whitening. Spectral whitening replaces the cross-spectrum with a unit amplitude spectrum. It is generally assumed that cross-terms have cancelled each other prior to spectral whitening. Cross-terms are peaks in the cross-correlation due to simultaneously acting noise sources, that is, spurious traveltime delays due to constructive interference of signal coming from different sources. Cancellation of these cross-terms is a requirement for the successful retrieval of interferometric receiver–receiver signal and results from ensemble averaging. In practice, ensemble averaging is replaced by integrating over sufficiently long time or averaging over several cross-correlation windows. Contrary to the general assumption, we show in this study that cross-terms are not required to cancel each other prior to spectral whitening, but may also cancel each other after the whitening procedure. Specifically, we derive an analytic approximation for the amplitude difference associated with the reversed order of cancellation and normalization. Our approximation shows that an amplitude decrease results from the reversed order. This decrease is predominantly non-linear at small receiver– receiver distances: at distances smaller than approximately two wavelengths, whitening prior to ensemble averaging causes a significantly stronger decay of the cross-spectrum.
Molecular Characterization of Enterohemorrhagic Escherichia coli Isolates from Cattle

ABSTRACT A total of 21 (4.3%) enterohemorrhagic E. coli strains were isolated by biochemical tests and identification of the eae+stx1+stx2+ genotype from 490 stool samples obtained from calves with diarrhea during 1-year period from a major farm in Tehran, Iran. All of the strains showed resistance to ampicillin, ciprofloxacin, trimethoprim, streptomycin, chloramphenicol and tetracycline, while 19% showed resistance to gentamicin. Out of 21 EHEC strains, 11 (53%) harbored class 1 integron. Two different amplification products, which were approximately 750 and 1,700 bp in size, were obtained from amplified variable regions (in-F/in-R primers) in 3 (14.3%) and 4 (19%) of the EHEC isolates, which corresponded to dfrA7(dihydrofolate reductase type I) and dfrA1/aadA1(dihydrofolate reductase/aminoglycoside adenyltransferase) resistance gene cassettes, respectively, and this was confirmed by sequencing. Genotyping analysis revealed a total of 16 pulsotypes that corresponded to 16 isolates with the similarity indices of 62% and 30% for the most and least similar isolates, respectively, 9 of which harbored class 1 integron. Analysis of pulsotypes showed an extensive diversity among the isolates harboring integron, which is indicative of a lack of any significant genetic relatedness among the isolates. No obvious relation could be deduced between integron content and special pulsotypes. The little data available on the genotyping patterns of EHEC isolates from cattle and their resistance gene contents emphasize the need to establish genotyping databases in order to monitor and source track the source of emergence and spread of new resistant and integron-carrying genotypes.
Privacy Concerns and Personality Traits Influencing Online Behavior: A Structural Model

The concept of privacy has proven difficult to analyze because of its subjective nature and susceptibility to psychological and contextual influences. This study challenges the concept of privacy as a valid construct for addressing individuals' concerns regarding online disclosure of personal information, based on the premise that underlying behavioral traits offer a more reliable and temporally stable measure of privacy-oriented behavior than do snapshots of environmentally induced emotional states typically measured by opinion polls. This study investigated the relationship of personality characteristics associated with individuals' general privacy-related behavior to their online privacy behaviors and concerns. Two latent constructs, Functional Privacy Orientation and Online Privacy Orientation, were formulated. Functional Privacy Orientation is defined as a general measure of individuals' perception of control over their privacy. It was measured using the factors General Disclosiveness, Locus of Control, Generalized Trust, Risk Orientation, and Risk Propensity as indicator variables. Online Privacy Orientation is defined as a measure of individuals' perception of control over their privacy in an online environment. It was measured using the factors Willingness to Disclose Online, Level of Privacy Concern, Information Management Privacy Concerns, and Reported Online Disclosure as indicator variables. A survey questionnaire that included two new instruments to measure online disclosure and a willingness to disclose online was used to collect data from a sample of 274 adults. Indicator variables for each of the latent constructs, Functional Privacy Orientation and Online Privacy Orientation, were evaluated using corrected item-total correlations, factor analysis, and coefficient alpha. The measurement models and relationship between Functional Privacy Orientation and Online Privacy Orientation were assessed using exploratory factor analysis and structural equation modeling respectively. The structural model supported the hypothesis that Functional Privacy Orientation significantly influences Online Privacy Orientation. Theoretical, methodological, and practical implications and suggestions for analysis of privacy concerns and behavior are presented.
X-irradiation of the Contusion Site Improves Locomotor and Histological Outcomes in Spinal Cord-Injured Rats

We have determined whether X-irradiation of the injury site can oppose tissue loss and improve recovery of locomotor function following contusion injury of the spinal cord. Contusion injury was produced in rats at the level of T10 with a weight drop device. Localized X-irradiation (20 Gy) of the injury site was performed at 20 min and 1, 2, 4, 7, and 17 days postinjury. Locomotor recovery was then determined with the 21-point Basso, Beattie, and Bresnahan (BBB) scale. X-irradiation enhanced recovery of locomotor function during a subsequent 6-week observation period when administered 20 min and 1 or 2 days following contusion injury (final BBB score approximately 7-8). X-irradiation at 4-17 days postinjury did not significantly affect final locomotor scores compared with unirradiated rats (final BBB score approximately 2), in marked contrast to previous studies where X-irradiation applied only at 17-18 days benefitted transection injury. The extent of recovery was directly related to measurements of sparing of spinal cord tissue at the contusion center. Because the treatment time window occurred earlier in contusion than reported for transection injury, the results suggest that contusion injury rapidly initiates underlying radiation-sensitive processes that occur only following a delay of several weeks after transection injury. Further optimization of X-ray treatment may lead to a useful therapeutic modality for use in spinal cord contusion injury.
Cesium-131 brachytherapy in high risk and recurrent head and neck cancers: first report of long-term outcomes

Purpose The feasibility and efficacy of re-irradiation using contemporary radiation techniques to treat recurrent head and neck cancer has been demonstrated but the role of brachytherapy is unclear. Here we describe the use of 131Cs brachytherapy with concurrent salvage surgery in 18 patients. Material and methods Eligible patients underwent maximal gross resection of the tumor with implantation of brachytherapy seeds delivering a minimum dose of 80 Gy to the tumor bed. Rates of overall survival, locoregional progression free survival, disease-free survival, and radiation-induced toxicity were analyzed. Results Retrospective Kaplan-Meier analysis shows median overall survival was 15 months and disease free survival was 12 months. Two patients developed grade 3 toxicity; all other complications were grade 1-2 with no grade 4 or 5 complications. Conclusions Compared to prior literature, our study shows comparable rates of survival with a decreased rate of radiation-induced toxicity.
Cephalometric evaluation of soft tissue changes after extraction of upper first premolars in class ΙΙ div 1 patients

Introduction: Tooth extraction to provide sufficient space, or camouflage of underlying skeletal problems is quite common in orthodontics. The present study evaluated soft tissue changes after upper first premolars extraction in class ΙΙ div 1 patients. Material and Methods: 20 cases (15 females, 5 males), with a mean age of 17.8±2.9 years with class ΙΙ div1 malocclusion and normal vertical height, who needed upper first premolars extraction were selected. Pre- and post-treatment lateral cephalometric radiographs were digitized. Image analysis was conducted by View Box 3.1. 1 software. Paired t-test was used for comparison of pre- and post-treatment results. Results: The relationship of upper and lower lip to E-line and B-line had significant reduction. Dental variables of U1-NA(mm), U1-NA(°), overjet and overbite showed statistically significant reduction. Interincisal angle had significant increase. There were no significant difference in lower incisor variables and skeletal variables like SN-GoGn and FMA. Conclusions: Extraction of upper first premolars in patients with class ΙΙ div 1 malocclusion resulted in normal position of lips as presented by Holdaway, Legan and Ricketts which play a role in aesthetic profile. However, the amount of lip retraction was different from patient to patient. Key words:Soft tissue, fixed orthodontic treatment, class ΙΙ div 1, upper first premolar extraction.
Supervillin (p205): A Novel Membrane-associated, F-Actin–binding Protein in the Villin/Gelsolin Superfamily

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here the purification and initial characterization of p205, a 205-kD protein from bovine neutrophil plasma membranes that binds to the sides of actin filaments in blot overlays. p205 is a tightly bound peripheral membrane protein that cosediments with endogenous actin in sucrose gradients and immunoprecipitates. Amino acid sequences were obtained from SDS-PAGE–purified p205 and used to generate antipeptide antibodies, immunolocalization data, and cDNA sequence information. The intracellular localization of p205 in MDBK cells is a function of cell density and adherence state. In subconfluent cells, p205 is found in punctate spots along the plasma membrane and in the cytoplasm and nucleus; in adherent cells, p205 concentrates with E-cadherin at sites of lateral cell–cell contact. Upon EGTA-mediated cell dissociation, p205 is internalized with E-cadherin and F-actin as a component of adherens junctions “rings. ” At later times, p205 is observed in cytoplasmic punctae. The high abundance of p205 in neutrophils and suspension-grown HeLa cells, which lack adherens junctions, further suggests that this protein may play multiple roles during cell growth, adhesion, and motility. Molecular cloning of p205 cDNA reveals a bipartite structure. The COOH terminus exhibits a striking similarity to villin and gelsolin, particularly in regions known to bind F-actin. The NH2 terminus is novel, but contains four potential nuclear targeting signals. Because p205 is now the largest known member of the villin/gelsolin superfamily, we propose the name, “supervillin. ” We suggest that supervillin may be involved in actin filament assembly at adherens junctions and that it may play additional roles in other cellular compartments.
Morphometry of the heart of budgerigars (Melopsittacus undulatus), Alisterus parrots (Alisterus s scapularis) and common buzzards (Buteo buteo).

In order to establish data and reference values for the thickness of the myocardium and the length of the left and the right ventricle of the avian heart, the hearts of 14 budgerigars (Melopsittacus undulatus), five Alisterus parrots (Alisterus s scapularis) (also known as Australian king parrot) and 10 common buzzards (Buteo buteo) of both sexes were examined according to a standard protocol. In order to compare the results of birds of different sizes all data were related to the size of the bird's body (length of the sternum) and the results of the measurements of the myocardial thickness in relation to the length of the heart. Results of different zones were compared by means of statistical methods within one species. Besides that a comparison between the different species was performed. Only minor significant differences were noted.
The Effects of the Level of Alertness During the Rest Period on Subsequent Performance

The aim of this study was to examine how to take an effective rest to prevent a decline in alertness at work. The relationship between alertness during the rest period and subsequent task performance were investigated. The electroencephalogram (EEG) during the rest period was classified into 3 types, and these types had a significant effect on performance after the rest period. Type 1 (increasing in theta, alpha 2, and beta 1 power) was the best one for carrying out the task, whereas performance gradually declined in Type 3 (no change in EEG activity). In the case of Type 1, the method that would relieve sleep inertia had a more positive impact on performance after the rest period.
KNOWLEDGE MANAGEMENT AND ENTERPRISE SYSTEMS ADOPTION BY SMES Predicting SMEs ’ adoption of enterprise systems

Purpose – The purpose of this paper is to develop a model that can be used to predict which small to medium-sized enterprises (SMEs) are more likely to become adopters of enterprise systems (ERP, CRM, SCM and e-procurement). Design/methodology/approach – Direct interviews were used to collect data from a random sample of SMEs located in the Northwest of England. Using logistic regression, 102 responses were analysed. Findings – The results reveal that the factors influencing SMEs’ adoption of enterprise systems are different from the factors influencing SMEs’ adoption of other previously studied information systems (IS) innovations. SMEs were found to be more influenced by technological and organisational factors than environmental factors. Moreover, the results indicate that firms with a greater perceived relative advantage, a greater ability to experiment with these systems before adoption, greater top management support, greater organisational readiness and a larger size are predicted to become adopters of enterprise systems. Research limitations/implications – Although this study focused on the factors that influence SMEs’ adoption of a set of enterprise systems (i.e. ERP, CRM, SCM and e-procurement), it fails to differentiate between factors that influence each of these systems. Practical implications – The model can be used to assist software vendors not only to develop marketing strategies that can target potential adopters, but also to develop strategies to increase the adoption of ES among SMEs. Originality/value – The paper contributes to the continuing research in IS innovations adoption/diffusion in the small business context.