Miracle-dz/UniFAR-FTUs huggingface dataset:
Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations

SUMMARY Major biogeochemical processes in the water columns of lakes and oceans are related to the activities of heterotrophic microbes, e.g., the mineralization of organic carbon from photosynthesis and allochthonous influx or its transport to the higher trophic levels. During the last 15 years, cultivation-independent molecular techniques have substantially contributed to our understanding of the diversity of the microbial communities in different aquatic systems. In parallel, the complexity of aquatic habitats at a microscale has inspired research on the ecophysiological properties of uncultured microorganisms that thrive in a continuum of dissolved to particulate organic matter. One possibility to link these two aspects is to adopt a “Gleasonian” perspective, i.e., to study aquatic microbial assemblages in situ at the population level rather than looking at microbial community structure, diversity, or function as a whole. This review compiles current knowledge about the role and fate of different populations of heterotrophic picoplankton in marine and inland waters. Specifically, we focus on a growing suite of techniques that link the analysis of bacterial identity with growth, morphology, and various physiological activities at the level of single cells. An overview is given of the potential and limitations of methodological approaches, and factors that might control the population sizes of different microbes in pelagic habitats are discussed.
A Hierarchical Account of Referential Accessibility

In this paper, we outline a theory of referential accessibility called Veins Theory (VT). We show how VT addresses the problem of "left satellites", currently a problem for stack-based models, and show that VT can be used to significantly reduce the search space for antecedents. We also show that VT provides a better model for determining domains of referential accessibility, and discuss how VT can be used to address various issues of structural ambiguity.
Sugammadex as a reversal agent for neuromuscular block: an evidence-based review

Sugammadex is the first clinical representative of a new class of drugs called selective relaxant binding agents. It has revolutionized the way anesthesiologists think about drug reversal. Sugammadex selectively binds rocuronium or vecuronium, thereby reversing their neuromuscular blocking action. Due to its 1:1 binding of rocuronium or vecuronium, it is able to reverse any depth of neuromuscular block. So far, it has been approved for use in adult patients and for pediatric patients over 2 years. Since its approval in Europe, Japan, and Australia, further insight on its use in special patient populations and specific diseases have become available. Due to its pharmacodynamic profile, sugammadex, in combination with rocuronium, may have the potential to displace succinylcholine as the “gold standard” muscle relaxant for rapid sequence induction. The use of rocuronium or vecuronium, with the potential of reverse of their action with sugammadex, seems to be safe in patients with impaired neuromuscular transmission, ie, neuromuscular diseases, including myasthenia gravis. Data from long-term use of sugammadex is not yet available. Evidence suggesting an economic advantage of using sugammadex and justifying its relatively high cost for an anesthesia-related drug, is missing.
Acute genital infection in guinea pigs: effect of recombinant interleukin-2 on herpes simplex virus type 2.

Human recombinant interleukin-2 (rIL-2) modifies infection with herpes simplex virus type 2 (HSV-2) in normal guinea pigs. Animals were injected sc with rIL-2 twice a day, beginning 24 hr before infection and continuing for three subsequent days. Guinea pigs were assigned to five different regimens in which rIL-2 was administered daily at dosages from 8 X 10(3) U/kg to 8 X 10(5) U/kg. After intravaginal inoculation with HSV-2, 83% of 24 control animals developed apparent genital herpes, and 5% had asymptomatic viral shedding. Animals receiving 4 X 10(4) U or 2 X 10(5) U or rIL-2/kg showed a significantly lower rate of infection (P less than .001 by chi 2) and a lower number of lesions and more rapid healing than did the infected animals in the control (untreated) group. The mortality in the untreated group (28%) was higher than in the animals in the 2 X 10(5) U/kg treatment group (7%). No difference was observed between the control, the 8 X 10(3) U/kg, and the 8 X 10(5) U/kg treatment groups with respect to severity of infection or mortality. Animals examined two to four weeks after inoculation had lymphocyte stimulation indices (induced with HSV-2 antigen) greater than 3.5 and titers of antibody between 2.5 log10 and 4.5 log10 by radioimmunoassay. In the disease-free animals, stimulation indexes were less than 2 and antibodies were undetectable. The mean stimulation index was 54 +/- 16 in the diseased control animals and 12 +/- 7 (P less than .05) in the 2 X 10(5) U/kg treatment group. (ABSTRACT TRUNCATED AT 250 WORDS)
Educational Differences in Smoking among Adolescents in Germany: What is the Role of Parental and Adolescent Education Levels and Intergenerational Educational Mobility?

Background: Adolescence is the period in which smoking onset usually occurs and the course for future socioeconomic status (SES) is set. However, because of the transitional nature of adolescence, it is questionable whether health inequalities are best measured by indicators of parental SES or rather by indicators of the adolescents’ own developing SES. We examine the independent effects of parental and adolescent education and intergenerational educational mobility on adolescent smoking behaviour while controlling for differences in parental and close friends’ smoking behaviour. Methods: The study is based on data from a subsample (12–17 years, n = 5,053) of the nationally representative German Health Interview and Examination Survey for Children and Adolescents (KiGGS). Participants reported their education level as well as their personal and close friends’ smoking behaviour. Information on parental education and smoking behaviour was obtained via parent interviews. Adolescent and parental education data were dichotomized (low/high), leading to four categories of intergenerational educational mobility: stable high, potentially upwardly mobile, potentially downwardly mobile, and stable low. Results: After adjustment for parental and close friends’ smoking behaviour, adolescent smoking habits were strongly related to their personal education level, but not that of their parents. Among boys, both stable low and downwardly mobile adolescents had a 2.7-fold increased risk of being a smoker compared with peers with a stable high education. Among girls, only those with a stable low education had a 2.2-fold increased risk of smoking. Among both genders, educational upward mobility was associated with significantly lower smoking rates compared with peers with a stable low education (boys: OR 0.32; 95% CI 0.20–0.53; girls: OR 0.52; 95% CI 0.37–0.73). Conclusions: Our results show that the risk of an adolescent smoking is influenced by their own education level rather than that of their parents. Educational upward mobility seems to be protective against becoming a smoker in youth. Boys who experience downward mobility tend to have a significantly higher inclination to smoke than their peers with a stable high education. These findings illustrate the potential public health benefits of investments in education and help identify high-risk groups for smoking onset.
Unexpected position‐dependent expression of H‐2 and β2‐microglobulin/lacz transgenes

In order to study sequences in volved in the developmentally regulated and tissue‐specific expression of the class I Major Histocompatibility Complex (MHC) genes, we have constructed several H‐2/lacZ transgenic lines in which the 5′ regulatory sequences of the H‐2Kb gene are linked to the Escherichia coli β‐galactosidase (lacZ) gene. In five H‐2/lacZ lines, the pattern of lacZ expression, detected histochemically varied greatly from line to line. None of the H‐2/lacZ transgenes were transcribed in cells normally expressing a high level of endogenous H‐2 molecules, although these H‐2 regulatory sequences have been shown to be sufficient to drive tissuespecific expression of other reporter genes. Interstingly, when constructs containing 5′ β2‐microglobulin (β2m) regulatory sequences linked to lacZ were used to derive transgenic lines, similar results were obtained. A survey of lacZ labeling in H‐2/lacZ and β2m/lacZ transgenic mice strongly suggests that these transgenes are very sensitive to position effect, lacZ expression being controlled by endogenous chromosomal regulatory elements specific for each insertion site. Here we describe the complex pattern of lacZ expression in the different transgenic lines during development; we discuss the unusual properties of these transgenes and underline their potential use for developmental studies and characterization of genomic sequences involved in spatiotemporal gene expression. © 1992 Wiley‐Liss, Inc.
A chromosome-level genome assembly of the Hispid cotton rat (Sigmodon hispidus), a model for human pathogenic virus infections

Background The cotton rat (Sigmodon hispidus), a rodent species native to the Americas, has emerged as a valuable laboratory model of infections by numerous human pathogens including poliovirus and respiratory syncytial virus (RSV). Results Here we report the first reference assembly of the cotton rat genome organized at a chromosomal level, providing annotation of 24,878 protein-coding genes. Data from PCR-free whole genome sequencing, linked-read sequencing and RNA sequencing from pooled cotton rat tissues were analyzed to assemble and annotate this novel genome sequence. Spectral karyotyping data using fluorescent probes derived from mouse chromosomes facilitated the assignment of cotton rat orthologs to syntenic chromosomes, comprising 25 autosomes and a sex chromosome in the haploid genome. Comparative phylome analysis revealed both gains and losses of numerous genes including immune defense genes against pathogens. We identified thousands of recently retrotransposed L1 and SINE B2 elements, revealing widespread genetic innovations unique to this species. Conclusions We anticipate that annotation and characterization of the first chromosome-level cotton rat genome assembly as described here will enable and accelerate ongoing investigations into its host defenses against viral and other pathogens, genome biology and mammalian evolution.
Effect of strength training with blood flow restriction on muscle power and submaximal strength in eumenorrheic women

Blood flow restriction (BFR) training stimulates muscle size and strength by increasing muscle activation, accumulation of metabolites and muscle swelling. This method has been used in different populations, but no studies have evaluated the effects of training on muscle power and submaximal strength (SS) in accounted for the menstrual cycle. The aim of this study was to analyse the effect of strength training (ST) with BFR on the muscle power and SS of upper and lower limbs in eumenorrheic women. Forty untrained women (18–40 years) were divided randomly and proportionally into four groups: (i) high‐intensity ST at 80% of 1RM (HI), (ii) low‐intensity ST at 20% of 1RM combined with partial blood flow restriction (LI + BFR), (iii) low‐intensity ST at 20% of 1RM (LI) and d) control group (CG). Each training group performed eight training sessions. Tests with a medicine ball (MB), horizontal jump (HJ), vertical jump (VJ), biceps curls (BC) and knee extension (KE) were performed during the 1st day follicular phase (FP), 14th day (ovulatory phase) and 26–28th days (luteal phase) of the menstrual cycle. There was no significant difference among groups in terms of the MB, HJ, VJ or BC results at any time point (P>0·05). SS in the KE exercise was significantly greater in the LI + BFR group compared to the CG group (P = 0·014) during the LP. Therefore, ST with BFR does not appear to improve the power of upper and lower limbs and may be an alternative to improve the SS of lower limbs of eumenorrheic women.
Open Research Online Creating good feelings about unhealthy food: children’s televised ‘advertised diet’ on the island of Ireland, in a climate of regulation

Childhood eating habits and associations with advertising persist through life. Obesity is high in Ireland, and is increasing worldwide. Links between food promotion and children’s diets are well-established, and the World Health Organisation has called for reduced marketing of foods high in fat, sugar and salt (HFSS) to children. In Ireland and the UK, statutory regulation restricts HFSS television advertising, but only during children’s programming – yet children view much television at other times. This study is the first to identify young children’s exposure to television food advertising on the island of Ireland (IoI), and its nature, with systematic sampling according to Irish audience panel research. Food advertisements were nutrient profiled and content analyses were conducted of marketing techniques. The IoI ‘advertised diet’ viewed by young children primarily features dairy and fast foods, pizza, sweets and chocolate, normalising this consumption and associating it with taste/aroma, fun, magic/ imagination, physical activity, humour and exaggerated pleasure. HFSS ads primarily featured taste/aroma, humour and novelty. Despite complying with statutory regulations, more than half of IoI food advertisements featured HFSS items; young children see over 1000 HFSS ads annually in the Republic of Ireland, nearly 700 in Northern Ireland. Policy implications for remedying children’s HFSS ad exposure include (i) applying food advertising restrictions to times when higher proportions of young children watch television – not just child-directed programming – as well as to digital media, (ii) employing a stricter nutrient profiling method and (iii) normalising children’s ‘advertised diet’ by exploring ways to advertise healthy foods. when high proportions of young children (4–6 years) view television, and systematic nutritional categorisations of foods as healthy/less healthy; (ii) differences in HFSS food advertising between the two jurisdictions on the IoI and (iii) persuasive techniques employed by advertisers to promote HFSS and healthier foods at times and on channels when data indicate young children are most likely to be viewing.
Comparing fully convolutional networks, random forest, support vector machine, and patch-based deep convolutional neural networks for object-based wetland mapping using images from small unmanned aircraft system

Deep learning networks have shown great success in several computer vision applications, but its implementation in natural land cover mapping in the context of object-based image analysis (OBIA) is rarely explored area especially in terms of the impact of training sample size on the performance comparison. In this study, two representatives of deep learning networks including fully convolutional networks (FCN) and patch-based deep convolutional neural networks (DCNN), and two conventional classifiers including random forest and support vector machine were implemented within the framework of OBIA to classify seven natural land cover types. We assessed the deep learning classifiers using different training sample sizes and compared their performance with traditional classifiers. FCN was implemented using two types of training samples to investigate its ability to utilize object surrounding information. Our results indicate that DCNN may produce inferior performance compared to conventional classifiers when the training sample size is small, but it tends to show substantially higher accuracy than the conventional classifiers when the training sample size becomes large. The results also imply that FCN is more efficient in utilizing the information in the training sample than DCNN and conventional classifiers, with higher if not similar achieved accuracy regardless of sample size. DCNN and FCN tend to show similar performance for the large sample size when the training samples used for training the FCN do not contain object surrounding label information. However, with the ability of utilizing surrounding label information, FCN always achieved much higher accuracy than all the other classification methods regardless of the number of training samples.
On media-based modulation using RF mirrors

Media-based modulation (MBM) is a recently proposed modulation scheme which uses radio frequency (RF) mirrors at the transmit antenna(s) in order to create different channel fade realizations based on their ON/OFF status. These complex fade realizations constitute the modulation alphabet. MBM has the advantage of increased spectral efficiency and performance. In this paper, we investigate the performance of some physical layer techniques when applied to MBM. Particularly, we study the performance of i) MBM with generalized spatial modulation (GSM), ii) MBM with mirror activation pattern (MAP) selection based on an Euclidean distance (ED) based metric, and iii) MBM with feedback based phase compensation and constellation rotation. Our results show that, for the same spectral efficiency, GSM-MBM can achieve better performance compared to MIMO-MBM. Also, it is found that MBM with ED-based MAP selection results in improved bit error performance, and that phase compensation and MBM constellation rotation increases the ED between the MBM constellation points and improves the performance significantly.
Vertigo: automatic performance-setting for Linux

Combining high performance with low power consumption is becoming one of the primary objectives of processor designs. Instead of relying just on sleep mode for conserving power, an increasing number of processors take advantage of the fact that reducing the clock frequency and corresponding operating voltage of the CPU can yield quadratic decrease in energy use. However, performance reduction can only be beneficial if it is done transparently, without causing the software to miss its deadlines. In this paper, we describe the implementation and performance-setting algorithms used in Vertigo, our power management extensions for Linux. Vertigo makes its decisions automatically, without any application-specific involvement. We describe how a hierarchy of performance-setting algorithms, each specialized for different workload characteristics, can be used for controlling the processor's performance. The algorithms operate independently from one another and can be dynamically configured. As a basis for comparison with conventional algorithms, we contrast measurements made on a Transmeta Crusoe-based computer using its built-in LongRun power manager with Vertigo running on the same system. We show that unlike conventional interval-based algorithms like LongRun, Vertigo is successful at focusing in on a small range of performance levels that are sufficient to meet an application's deadlines. When playing MPEG movies, this behavior translates into a 11%-35% reduction of mean performance level over LongRun, without any negative impact on the framerate. The performance reduction can in turn yield significant power savings.
Quantitative determination of trichothecenes in breadsticks by gas chromatography-triple quadrupole tandem mass spectrometry

Breadsticks are pencil-sized sticks of dry bread widely consumed as a pre-meal appetiser. They are basically wheat-based snacks, which makes them a good matrix to evaluate mycotoxin contamination, since wheat is very susceptible to fungal attack. In this sense, the fast, selective and sensitive gas chromatography-triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS) method proposed here allows simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol, fusarenon-X, diacetoxyscirpenol, nivalenol, neosolaniol, HT-2 and T-2 toxin in breadsticks after QuEChERS extraction and clean-up. The performance of the method was assessed with respect to European Commission Regulations by studying the selectivity and specificity, limit of detection (LOD), limit of quantitation (LOQ), linearity, matrix effect, accuracy, precision and trueness. Satisfactory results in terms of validation parameters were obtained for all selected mycotoxins (recovery range of 70–110%, RSD < 10%, LOQ <40 µg kg–1). The trueness of the method was supported by using certified reference material (DON 1062 ± 110 µg kg–1). The method was successfully used to evaluate the occurrence of the studied Fusarium toxins in 61 breadstick samples. A total of 64% of the samples showed mycotoxin contamination, DON being the most frequently detected toxin. Nonetheless, mean levels obtained were far below the maximum levels permitted by European Union legislation. An additional goal was to carry out a risk-characterisation approach to DON by comparing probable daily intake and provisional maximum tolerable daily intake (PMTDI). Graphical Abstract
Human Perception and Performance Redundancy Gain in the Simon Task: Does Increasing Relevant Activation Reduce the Effect of Irrelevant Activation?

In the present study, we tested the idea that relevant and irrelevant location-based activations are combined when selecting a response in the Simon task. For this purpose, we manipulated the strength of the relevant activation by using single versus redundant relevant stimuli to indicate the correct response. Assuming that relevant and irrelevant activations are summed during the decision process, the Simon effect should be reduced in the presence of the stronger relevant activation produced by redundant stimuli. Contrary to this expectation, the results revealed that the Simon effect was larger overall with redundant stimuli than with single stimuli. Importantly, distributional analyses indicated that the Simon effect was not reduced for redundant stimuli even when controlling for the opportunity for irrelevant location activation to dissipate after stimulus onset. These results are thus inconsistent with accounts in which the summation of decision-level activations is the cause of the Simon effect. Instead, these results are consistent with the idea that redundancy and irrelevant location information influence activations at different levels. Considering the findings of previous studies, the most likely account is that redundancy affects decision-level activations whereas relevant and irrelevant activation are integrated at the motor level to produce the Simon effect (i.e., decision locus of redundancy gain and motor locus of Simon effect). Public Significance Statement People are often required to select responses in the presence of multiple sources of information, both relevant and irrelevant. In the present study, we examined how activations from these different sources are combined. The results suggest that activations from multiple sources of relevant information are combined during decision making, whereas activation from irrelevant location information influences the subsequent process of executing the motor response.