Miracle-dz/UniFAR-FTUs huggingface dataset:
Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae

Ribosome biogenesis is a multi-step process that couples cell growth with cell proliferation. Although several large-scale analysis of pre-ribosomal particles have identified numerous trans-acting factors involved in this process, many proteins involved in pre-rRNA processing and ribosomal subunit maturation have yet to be identified. Las1 was originally identified in Saccharomyces cerevisiae as a protein involved in cell morphogenesis. We previously demonstrated that the human homolog, Las1L, is required for efficient ITS2 rRNA processing and synthesis of the 60S ribosomal subunit. Here, we report that the functions of Las1 in ribosome biogenesis are also conserved in S. cerevisiae. Depletion of Las1 led to the accumulation of both the 27S and 7S rRNA intermediates and impaired the synthesis of the 60S subunit. We show that Las1 co-precipitates mainly with the 27S rRNA and associates with an Nsa1 and Rix1-containing pre-60S particle. We further identify Grc3 as a major Las1-interacting protein. We demonstrate that the kinase activity of Grc3 is required for efficient pre-rRNA processing and that depletion of Grc3 leads to rRNA processing defects similar to the ones observed in Las1-depleted cells. We propose that Las1 and Grc3 function together in a conserved mechanism to modulate rRNA processing and eukaryotic ribosome biogenesis.
Slaughterhouse Wastewater Treatment by Combined Chemical Coagulation and Electrocoagulation Process

Slaughterhouse wastewater contains various and high amounts of organic matter (e.g., proteins, blood, fat and lard). In order to produce an effluent suitable for stream discharge, chemical coagulation and electrocoagulation techniques have been particularly explored at the laboratory pilot scale for organic compounds removal from slaughterhouse effluent. The purpose of this work was to investigate the feasibility of treating cattle-slaughterhouse wastewater by combined chemical coagulation and electrocoagulation process to achieve the required standards. The influence of the operating variables such as coagulant dose, electrical potential and reaction time on the removal efficiencies of major pollutants was determined. The rate of removal of pollutants linearly increased with increasing doses of PACl and applied voltage. COD and BOD5 removal of more than 99% was obtained by adding 100 mg/L PACl and applied voltage 40 V. The experiments demonstrated the effectiveness of chemical and electrochemical techniques for the treatment of slaughterhouse wastewaters. Consequently, combined processes are inferred to be superior to electrocoagulation alone for the removal of both organic and inorganic compounds from cattle-slaughterhouse wastewater.
Developing Self-Care Practices in a Trauma Treatment Course

This article describes the development of self-care practices of social work students who were part of a larger study of students’ experiences in a graduate course on the treatment of trauma. Consensual qualitative research methods were used to analyze 17 participant journals submitted at 4 times during the course. Findings indicated that students benefited from being introduced to evidence-based practices for mediating the stress of trauma education, including journaling and mindfulness-based stress reduction. Although students reported struggling with developing effective self-care practices, they demonstrated an ability to integrate self-care into their professional practice behaviors. The authors review recommendations for research and pedagogy of self-care practices in courses on trauma treatment.
Teaching Intercultural Competence: Challenges and Opportunities

This paper presents a case study analysis of Intercultural Studies electives delivered to students as part of a Business Studies degree programme in Dundalk Institute of Technology, Ireland. A survey captured students’ perceptions of the existing Intercultural Studies electives. Much of the literature endorses the importance of intercultural competence for students in general, and business graduates in particular, in a globalised world. The nature and range of active learning methods currently employed on this elective are also cited in the literature as being conducive to building intercultural competence. This was further evidenced in the findings from the student survey conducted. The conclusions of the case study present some clear opportunities regarding both the challenges and the importance of building awareness, knowledge and skills among graduates using a range of relevant teaching and learning methods.
Forensic aspects of the metabolism and excretion of cannabinoids following oral ingestion of cannabis resin

Following oral ingestion of cannabis resin, δ9‐THC−11−oic acid and its O‐ester glucuronide were detected using RIA and combined hplc/RIA and shown to be major plasma metabolites of δ9‐THC. δ9‐THC−11−oic acid was not excreted in the urine in significant concentrations, the glucuronide conjugate being the major urinary metabolite detected. δ9‐THC metabolites were detected in blood for up to 5 days and in urine for up to 12 days following a single oral dose of δ9‐THC (20 mg). Estimates for the half life of δ9‐THC−11−oic acid and its glucuronide in plasma, and total metabolites in urine have been obtained. Interpretation of blood or urine total cannabinoid levels is most difficult, however, drug/metabolite ratios and metabolite/metabolite ratios may have potential for indicating recent cannabis use.
Exploring the alignment between postsecondary education programs and earnings: An examination of 2005 Ontario graduates

This study examines the influence that field of study and level of postsecondary education have on the earnings of recent graduates in Ontario. Attention is also given to gender as enrolment in post-secondary education programs remains segregated by gender, leading to differences in occupational choice and earnings disparities between men and women. Results suggest that graduates of applied and technical programs obtain higher earnings within two years of graduation. University graduates also fare better than college and trades graduates; however, male graduates of trades programs are found to obtain higher earnings than college graduates. This study provides important information for policy officials involved with allocating government funding to postsecondary education. Results may also be of interest to administrators of college, trades, and university programs who are concerned with enrolment across different fields of study and students seeking information on school-to-work transitions.
The reliability and validity of the Family Affluence Scale

This article investigates the statistical reliability and validity of a commonly used proxy measure of children's socio‐economic status, the Family Affluence Scale (Version II). The article is based on a secondary analysis of data taken from a stratified sample of 673 children aged 9–10 years selected from 35 schools. Reliability analysis, confirmatory factor analysis and an exploratory factor analysis are used to assess the various aspects of reliability and validity of the Family Affluence Scale. The findings indicate that the scale has a number of strengths and weaknesses. Its major strengths are that it obtains direct responses from individual children and that it shows some construct validity as a measure of socio‐economic status not captured by other widely used indicators, namely: small area indices of deprivation and ‘eligibility’ for free school meals. However, its flaws include poor measurement reliability and variable construct validity. The article concludes that while the Family Affluence Sca.
Caspase activation during phorbol ester-induced apoptosis requires ROCK-dependent myosin-mediated contraction

Treatment of cells with phorbol ester, phorbol-12-myristate-13-acetate (PMA), triggers differentiation or apoptosis, depending on the cell type. In this study, we used an erythroblastic cell line, TF-1, to investigate the molecular mechanism that determines the cell fate in response to PMA exposure. Upon PMA treatment in the presence of serum or lysophosphatidic acid (LPA), TF-1 cells exhibited contraction followed by apoptosis. By contrast, under serum-free conditions, cells became adherent and survived after PMA treatment. Here, we show that the pathway of Rho kinase (ROCK)/myosin light chain (MLC) phosphorylation/myosin-mediated contraction was activated in PMA-induced apoptotic cells in serum-containing medium, but not in the adherent and survived cells. Pretreatment of cells with a specific ROCK inhibitor, Y27632, not only abrogated MLC phosphorylation and membrane contraction, but also prevented PMA-induced activation of caspase-3 and subsequent cell death, indicating that ROCK-dependent myosin-mediated contraction elicits an upstream signal required for caspase-3 activation in PMA-induced apoptosis. Interestingly, we further found that caspases-8 and -10 are the initiator caspases in PMA-induced apoptosis and a ROCK-dependent enhancement of specific complex formation between the Fas-associated death domain (FADD) and pro-caspase-10 in pro-apoptotic cells. In summary, these results revealed that, following PMA treatment, the upregulation of the RhoA/ROCK pathway contributes to a cellular context that switches-on myosin-mediated contraction, which provides a mechanism for triggering apoptotic induction mediated by caspase-8 and -10.
Clostridium difficile-associated diarrhea in a region of Quebec from 1991 to 2003: a changing pattern of disease severity

Background: Recent reports suggest that Clostridium difficile colitis may be evolving into a more severe disease. During the second half of 2002 we noted an increase in the number of patients with severe C. difficile-associated diarrhea (CDAD) in our institution. We describe cases of CDAD at our institution over a 13-year period and investigate changes in illness severity. Methods: We undertook a retrospective chart review of all cases of CDAD diagnosed at the Centre hospitalier universitaire de Sherbrooke from Jan. 1, 1991, to Dec. 31, 2003. Because the hospital serves a well-defined population of Quebec, we were also able to calculate population-based incidence during this period. We abstracted data on individual patients from patient charts and from hospital and pharmacy computer databases. We defined cases of CDAD as having a positive C. difficile cytotoxicity assay result, or endoscopic or histopathological evidence of pseudomembranous colitis. A case was considered complicated if one or more of the following was observed: megacolon, perforation, colectomy, shock requiring vasopressor therapy, or death within 30 days after diagnosis. Results: A total of 1721 cases of CDAD were diagnosed during the study period. The incidence increased from 35.6 per 100 000 population in 1991 to 156.3 per 100 000 in 2003; among patients aged 65 years or more, it increased from 102.0 to 866.5 per 100 000. The proportion of cases that were complicated increased from 7.1% (12/169) in 1991–1992 to 18.2% (71/390) in 2003 (p < 0.001), and the proportion of patients who died within 30 days after diagnosis increased from 4.7% (8/169) in 1991– 1992 to 13.8% (54/390) in 2003 (p < 0.001). A high leukocyte count (20.0 х 109/L or greater) and an elevated creatinine level (200 μmol/L or greater) were strongly associated with adverse outcomes: in 2003, 45 (40.9%) of 110 patients with a high leukocyte count or creatinine level, or both, had complicated CDAD and 28 (25.5%) died within 30 days after diagnosis. After adjustment for age and other confounding factors, patients initially given oral vancomycin therapy had a risk of progression to complicated CDAD that was 79% lower than the risk among patients initially treated with metronidazole (adjusted odds ratio 0.2, 95% confidence interval 0.06–0.8, p = 0.02). Interpretation: An epidemic of CDAD with an increased case-fatality rate has had important consequences on the elderly population of our region. Our observational data suggest that the equivalence of vancomycin and metronidazole in the treatment of CDAD needs to be questioned.
Factor structure of the Inventory of Parent Accommodations of Children’s Symptoms (IPACS) in a community sample

ABSTRACT Objective: To examine the factor structure of the Inventory of Parent Accommodations of Children’s Symptoms (IPACS) among a community sample of healthy children. Method: An online survey was completed by 220 parents ages 18-65 (M = 34.49; 96% female) for a study examining the effects of parent behaviors on frequency of general sickness and pain complaints (e.g., headaches and stomachaches) in otherwise healthy children. The survey included the IPACS, a measure of parent behaviors and responses to sickness or pain complaints, as well as items that assessed sociodemographics and frequency of sickness and pain complaints in children. Results: Exploratory factors analysis of the IPACS revealed a 12-item measure yielding three discrete, yet related subscales. Additionally, parent accommodations were slightly associated with an increase in sickness and pain complaints in children (r (220) = . 153, p<. 05). Conclusions: These findings suggest that parent behaviors during general sickness and pain complaints may serve as clinically relevant information that can be used to inform interventions that target specific parent behaviors that might exacerbate symptoms. Future research should examine the convergent validity of this measure with other measures of parenting behavior, as well as the specific utility of the IPACS in chronic illness and pain populations.
Hosea 1—3 in Twentieth-Century Scholarship

Throughout the twentieth century, critical scholarship on the book of Hosea has focused overwhelmingly on the marriage metaphor in Hosea 1—3. Scholars often saw these chapters as establishing the primary interpretive issues for the message of the prophet and the book as a whole, although a lack of consensus concerning even the most basic exegetical issues remains. Newer studies have rightly pushed beyond this isolation of Hosea 1—3. This article surveys the major trends of the modern interpretation of these chapters, with particular attention to the second half of the twentieth century. From the early 1900s to the 1980s, critical works focused primarily on the biographical reconstruction of the prophet and his family life, as well as related historical and form-critical concerns. From the 1930s forward, such study was particularly concerned to read Hosea 1—3 against the background of a purported sexualized Baal cult in eighth-century Israel. Beginning in the 1980s, feminist-critical readings of Hosea 1—3 came to occupy a prominent position. In subsequent years, these concerns have been complemented by an emerging emphasis on metaphor theory, as well as newer kinds of literary, book-oriented, and socio-historical analyses. A follow-up article will treat recent scholarship on Hosea 4—14.
Comparison of laser scanning and photogrammetry and their use for digital recording of cultural monument case study: Byzantine land walls-istanbul

Abstract. 3D modeling of cultural monuments is very crucial issue for preparing restoration projects. However, it has challenges such as data acquisition, preparation and processing. 3D modeling of objects can be time consuming and may include some difficulties due to the complexity of the structures. 3D terrestrial laser (TLS) scanning technique is one of the reliable and advantageous methods for 3D reconstruction of monuments. This technique is commonly acknowledged due to its accuracy, speed and flexibility. But the suitability and capability of this technique depends on proper usage, and good survey planning. Magnificent developments in highresolution digital sensor technologies leaded to manufacturing of new camera systems. Parallel to these innovations, development of computer systems and image processing techniques made enable to obtain multiple image-based 3D object models. In the presented study, TLS method has been compared to conventional photogrammetric and image-based dense matching methods. Automatic dense point creation has been realized by our developed algorithm and PIXEL-PHOTO software which generates 3D point clouds from stereo images. The reliability and encountered problems during point cloud measurement process have been discussed. The study area has been chosen as historical Byzantine Land Walls of Istanbul, which constitute a remarkable area defining the ancient city’s historical peninsula.
Aspirin and Urinary 11-Dehydrothromboxane B2 in African American Stroke Patients

Background and Purpose— The aim of the study was to evaluate the relationship between daily aspirin use and urinary excretion of a stable thromboxane metabolite, 11-dehydrothromboxane B2 (11-DTB2), in African American stroke patients. Methods— Subjects were a subgroup of those screened for the African American Antiplatelet Stroke Prevention Study. Subjects were within 4 months of noncardioembolic ischemic stroke and were not being treated with anticoagulants. Antithrombotic therapy at the time of urine collection varied according to the practice patterns of various attending physicians who treated the patients during their acute strokes. 11-DTB2 was measured by enzyme immunoassay in random urine samples 1 to 4 months after the stroke. Results— Eighty-seven of 92 patients enrolled were able to give a urine sample at the time of enrollment. There were 51 men and 36 women aged 36 to 87 (mean 62) years. On the basis of antithrombotic treatment before the sample collection, we divided patients into 4 groups: (1) 16 patients treated with no aspirin (no antithrombotic drugs [n=4] or ticlopidine [n=12]), (2) 21 patients treated with 81 to 325 mg aspirin per day (81 mg/d [n=2], 325 mg/d [n=19]), (3) 20 patients treated with 650 mg aspirin per day, and (4) 30 patients treated with 975 to 1300 mg aspirin per day (975 mg/d [n=2] and 1300 mg/d [n=28]). In patients taking daily aspirin at any dose, the median urinary 11-DTB2 was 783 pg/mg creatinine compared with 1386 pg/mg creatinine in patients not taking daily aspirin (P =0.01 by Wilcoxon rank sum test). In multivariate regression analysis, aspirin use remained significantly associated with lower urinary 11-DTB2 (P =0.008). There was no dose-response effect between the 3 aspirin dose groups and urinary 11-DTB2 (P =0.70). Conclusions— In African American stroke patients, aspirin use is associated with significantly lower urinary 11-DTB2 independent of other vascular factors, and there does not appear to be a dose-response effect for aspirin doses of 325 to 1300 mg daily. The clinical significance of these finding remains to be determined.
ZNF198 Stabilizes the LSD1–CoREST–HDAC1 Complex on Chromatin through Its MYM-Type Zinc Fingers

Histone modifications in chromatin regulate gene expression. A transcriptional co-repressor complex containing LSD1–CoREST–HDAC1 (termed LCH hereafter for simplicity) represses transcription by coordinately removing histone modifications associated with transcriptional activation. RE1-silencing transcription factor (REST) recruits LCH to the promoters of neuron-specific genes, thereby silencing their transcription in non-neuronal tissues. ZNF198 is a member of a family of MYM-type zinc finger proteins that associate with LCH. Here, we show that ZNF198-like proteins are required for the repression of E-cadherin (a gene known to be repressed by LSD1), but not REST-responsive genes. ZNF198 binds preferentially to the intact LCH ternary complex, but not its individual subunits. ZNF198- and REST-binding to the LCH complex are mutually exclusive. ZNF198 associates with chromatin independently of LCH. Furthermore, modification of HDAC1 by small ubiquitin-like modifier (SUMO) in vitro weakens its interaction with CoREST whereas sumoylation of HDAC1 stimulates its binding to ZNF198. Finally, we mapped the LCH- and HDAC1–SUMO-binding domains of ZNF198 to tandem repeats of MYM-type zinc fingers. Therefore, our results suggest that ZNF198, through its multiple protein-protein interaction interfaces, helps to maintain the intact LCH complex on specific, non-REST-responsive promoters and may also prevent SUMO-dependent dissociation of HDAC1.
Characterization of metabolic syndrome among forensic psychiatric inpatients

Schizophrenia and other psychotic disorders seem to be linked with metabolic syndrome. Metabolic syndrome is an important preceding factor for Type 2 diabetes mellitus and cardiovascular diseases. The objective of this study was to investigate the prevalence of metabolic syndrome and its risk determinants among psychotic forensic psychiatric inpatients with comorbidities of antisocial personality or alcohol dependence. The study sample consisted of 221 forensic psychiatric inpatients in Finland, with a median age of 41 years, of which 92% had a diagnosis of schizophrenia or a related psychosis. The patients had been on constant second-generation antipsychotic medication for over six months. The use of clozapine (OR 8.1), quetiapine (OR 7.7), and olanzapine (OR 3.6) was associated with the occurrence of metabolic syndrome. The beneficial high density lipoprotein cholesterol profile was correlated with the use of selective serotonin uptake inhibitor (SSRI), and with a diagnosis of alcohol dependence, even after alcohol abstinence. Metabolic syndrome was associated especially with the use of clozapine and quetiapine. Our novel finding was that SSRI use and previous diagnosis of alcohol dependence may have a lowering effect on the prevalence of metabolic syndrome.
Sound change in the individual: Effects of exposure on cross-dialect speech processing

Abstract Speech perception is highly robust to variation, but familiarity with a particular source of variation can nevertheless lead to significant processing benefits. In the domain of cross-dialect speech perception, familiar local and standard varieties have been shown to facilitate lexical and semantic processing relative to unfamiliar dialects. However, more recent research suggests that individuals with exposure to both a local non-standard variety and a regional or national standard variety exhibit a mix of lexical processing costs and benefits, suggesting that familiarity with multiple different linguistic systems can result in both independent processing benefits for each variety as well as competition among variable multi-dialect representations. In an exemplar model of language processing, this complex pattern of results suggests several loci for sound change within an individual language user. Although the processing benefits associated with the local variety may contribute to long-term maintenance of variation through divergence from a regional or national standard, the processing benefits associated with the standard may contribute to dialect leveling and convergence towards the standard. Competition between these forces for maintenance and leveling will be observed most strongly in individuals with extensive exposure to both a non-standard local variety and a regional or national standard.
Rationalization of Activity Cliffs of a Sulfonamide Inhibitor of DNA Methyltransferases with Induced-Fit Docking

Inhibitors of human DNA methyltransferases (DNMT) are of increasing interest to develop novel epi-drugs for the treatment of cancer and other diseases. As the number of compounds with reported DNMT inhibition is increasing, molecular docking is shedding light to elucidate their mechanism of action and further interpret structure–activity relationships. Herein, we present a structure-based rationalization of the activity of SW155246, a distinct sulfonamide compound recently reported as an inhibitor of human DNMT1 obtained from high-throughput screening. We used flexible and induce-fit docking to develop a binding model of SW155246 with a crystallographic structure of human DNMT1. Results were in excellent agreement with experimental information providing a three-dimensional structural interpretation of ‘activity cliffs’, e.g., analogues of SW155246 with a high structural similarity to the sulfonamide compound, but with no activity in the enzymatic assay.