Miracle-dz/UniFAR-FTUs huggingface dataset:
The Impact of Anonymity on Perceptions of Source Credibility and Influence in Computer-Mediated Group Communication: A Test of Two Competing Hypotheses

As scholars and practitioners have endeavored to develop computer-based tools that foster effective communication and collaboration in groups, anonymity has played a key role. Anonymity purportedly minimizes status differences, liberates team members from a fear of retribution, and makes members feel more comfortable contributing to discussions. Yet these benefits may be outweighed by the impact of anonymity on receiver perceptions and behavior. Two competing hypotheses, drawn from adaptive structuration theory, were tested in this study to determine the impact of anonymity on receiver perceptions of sources and messages in computer-mediated group communication. The results of the multilevel models offer evidence in support of the discounting hypothesis and suggest that anonymity provided by electronic meeting systems may undermine source credibility and influence.
Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons

NTera 2/cl.D1 (NT2) cells, a human teratocarcinoma cell line, were manipulated following retinoic acid treatment to yield greater than 95% pure cultures of neuronal cells (NT2-N cells). The commitment of NT2-N cells to a stable neuronal phenotype is irreversible as judged by the lack of mitotic activity or phenotypic reversion over a period of 2 months in culture. Furthermore, NT2-N cells express a variety of neuronal markers including many neuronal cytoskeletal proteins, secretory markers, and surface markers. NT2-N cells resemble primary neuronal cultures from rodents morphologically and in density of process outgrowth and, like primary neurons, go on to elaborate processes that differentiate into axons and dendrites. This culture method yields sufficient highly differentiated postmitotic NT2-N cells for both biochemical and molecular biological studies. Indeed, when undifferentiated NT2 cells were stably transfected with a beta- galactosidase (beta-gal) expression plasmid, beta-gal expression was shown to be present in both undifferentiated NT2 and postmitotic NT2-N cells. Thus, the ability to transfect expression plasmids into undifferentiated NT2 cells will allow the introduction of normal and mutant gene products into cells that can then be induced to become stable, postmitotic human neurons. We conclude that NT2 cells and NT2-N cells represent a unique model system for studies of human neurons, and a novel vehicle for the expression of diverse gene products in terminally differentiated polarized neurons.
Understanding the Relationship between Reading Fluency and Reading Comprehension: Fluency Strategies as a Focus for Instruction

Understanding the relationship between reading fluency and comprehension is important. Reading fluency strategies can be used to help increase overall reading comprehension in students. Five students and one parent participated in the study of how repeated readings impact overall reading comprehension. Students were exposed to a set of reading passages that were read each week, and data was collected. At the end of the school year, students were given a reading comprehension post assessment to compare the results from September. When analyzing the data, it was clear that the repeated reading strategy was successful in increasing reading comprehension among the students. These findings suggest that fluency strategies such as repeated reading should be a major focus of instruction for students. Document Type Thesis Degree Name MS in Literacy Education Department Education Subject Categories Education This thesis is available at Fisher Digital Publications: http://fisherpub.sjfc. edu/education_ETD_masters/224 Understanding the Relationship between Reading Fluency and Reading Comprehension: Fluency Strategies as a Focus for Instruction
A CHANDRA OBSERVATION OF 3C 288—REHEATING THE COOL CORE OF A 3 keV CLUSTER FROM A NUCLEAR OUTBURST at z = 0.246

We present results from a 42 ks Chandra/ACIS-S observation of the transitional FR I/FR II radio galaxy 3C 288 at z = 0.246. We detect ∼3 keV gas extending to a radius of ∼0.5 Mpc with a 0.5–2.0 keV luminosity of 6.6 × 1043 erg s−1, implying that 3C 288 lies at the center of a poor cluster. We find multiple surface brightness discontinuities in the gas indicative of either a shock driven by the inflation of the radio lobes or a recent merger event. The temperature across the discontinuities is roughly constant with no signature of a cool core, thus disfavoring either the merger cold front or sloshing scenarios. We argue therefore that the discontinuities are shocks due to the supersonic inflation of the radio lobes. If they are shocks, the energy of the outburst is ∼1060 erg, or roughly 30% of the thermal energy of the gas within the radius of the shock, assuming that the shocks are part of a front produced by a single outburst. The cooling time of the gas is ∼108 yr, so that the energy deposited by the nuclear outburst could have reheated and efficiently disrupted a cool core.
Preconceptional Iron Intake and Gestational Diabetes Mellitus

Our objective was to assess the impact of preconceptional heme and non-heme iron on gestational diabetes mellitius (GDM) in the Boston University Slone Epidemiology Birth Defects Study (BDS). This retrospective cohort analysis included 7229 participants enrolled in the BDS between 1998 and 2008 who gave birth to non-malformed infants and were free of pre-existing diabetes. All data were collected through structured interviews conducted within 6 months of delivery. Calorie-adjusted and multivariable odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression models. Preconceptional dietary heme iron was modestly associated with an elevated risk of GDM among those (multivariable OR comparing the fifth quintile to the first: 1.55; 95% CI 0.98, 2.46). Conversely, preconceptional dietary non-heme iron was associated with a decreased risk of GDM among those in the fifth quintile of intake compared to the first (multivariable OR: 0.48; 95% CI 0.28, 0.81). Women who consumed supplemental iron during preconception also had a decreased risk of GDM (multivariable OR: 0.78; 95% CI 0.60, 1.02). In conclusion, our data support a positive association between preconceptional heme iron intake and GDM and an inverse association between preconceptional non-heme iron intake from foods and preconceptional intake from supplements.
High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells.

AIM To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis. METHODS The impact of miR-196a on the restriction targets HoxA7, HoxB8, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction (RT-PCR) after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectal cancer samples, mucosa samples and diverse cancer cell lines was quantified by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo. RESULTS HoxA7, HoxB8, HoxC8 and HoxD8 were restricted by miR-196a in a dose-dependent and gene-specific manner. High levels of miR-196a activated the AKT signaling pathway as indicated by increased phosphorylation of AKT. In addition, high levels of miR-196a promoted cancer cell detachment, migration, invasion and chemosensitivity towards platin derivatives but did not impact on proliferation or apoptosis. Furthermore, miR-196a increased the development of lung metastases in mice after tail vein injection. CONCLUSION miR-196a exerts a pro-oncogenic influence in colorectal cancer.
Structural and Functional Characterization of a Complex between the Acidic Transactivation Domain of EBNA2 and the Tfb1/p62 Subunit of TFIIH

Infection with the Epstein-Barr virus (EBV) can lead to a number of human diseases including Hodgkin's and Burkitt's lymphomas. The development of these EBV-linked diseases is associated with the presence of nine viral latent proteins, including the nuclear antigen 2 (EBNA2). The EBNA2 protein plays a crucial role in EBV infection through its ability to activate transcription of both host and viral genes. As part of this function, EBNA2 associates with several host transcriptional regulatory proteins, including the Tfb1/p62 (yeast/human) subunit of the general transcription factor IIH (TFIIH) and the histone acetyltransferase CBP(CREB-binding protein)/p300, through interactions with its C-terminal transactivation domain (TAD). In this manuscript, we examine the interaction of the acidic TAD of EBNA2 (residues 431–487) with the Tfb1/p62 subunit of TFIIH and CBP/p300 using nuclear magnetic resonance (NMR) spectroscopy, isothermal titration calorimeter (ITC) and transactivation studies in yeast. NMR studies show that the TAD of EBNA2 binds to the pleckstrin homology (PH) domain of Tfb1 (Tfb1PH) and that residues 448–471 (EBNA2448–471) are necessary and sufficient for this interaction. NMR structural characterization of a Tfb1PH-EBNA2448–471 complex demonstrates that the intrinsically disordered TAD of EBNA2 forms a 9-residue α-helix in complex with Tfb1PH. Within this helix, three hydrophobic amino acids (Trp458, Ile461 and Phe462) make a series of important interactions with Tfb1PH and their importance is validated in ITC and transactivation studies using mutants of EBNA2. In addition, NMR studies indicate that the same region of EBNA2 is also required for binding to the KIX domain of CBP/p300. This study provides an atomic level description of interactions involving the TAD of EBNA2 with target host proteins. In addition, comparison of the Tfb1PH-EBNA2448–471 complex with structures of the TAD of p53 and VP16 bound to Tfb1PH highlights the versatility of intrinsically disordered acidic TADs in recognizing common target host proteins.
Cod Liver Oil Supplement Consumption and Health: Cross-sectional Results from the EPIC-Norfolk Cohort Study

Supplement users (SU) make healthy lifestyle choices; on the other hand, SU report more medical conditions. We hypothesised that cod liver oil (CLO) consumers are similar to non-supplement users, since CLO use might originate from historical motives, i.e., rickets prevention, and not health consciousness. CLO consumers were studied in order to identify possible confounders, such as confounding by indication. The European Prospective Investigation into Cancer (EPIC) investigates causes of chronic disease. The participants were 25,639 men and women, aged 40–79 years, recruited from general practices in Norfolk, East-Anglia (UK). Participants completed questionnaires and a health examination between 1993 and 1998. Supplement use was measured using 7-day diet diaries. CLO was the most common supplement used, more prevalent among women and associated with not smoking, higher physical activity level and more favourable eating habits. SU had a higher occurrence of benign growths and bone-related diseases, but CLO was negatively associated with cardiovascular-related conditions. Although the results of SU characteristics in EPIC-Norfolk are comparable with studies worldwide, the CLO group is different from SU in general. Confounding by indication takes place and will need to be taken into account when analysing prospective associations of CLO use with fracture risk and cardiovascular diseases.
Measuring Emotional Intelligence and Related Constructs

Schutte and Malouff (psychology, Nova Southeastern U., Florida) have compiled a number of questionnaires for assessing emotional attributes such as social awareness, expressivity, emotional control, coping styles, optimism and pessimism, impulsiveness, and interpersonal reactivity. For each test they include a discussion of its purpose and development, relationship to emotional intelligence, administration and scoring, sample and cutoff scores, reliability, and validity. Double spaced.
Individual Differences in Warning Perception: The Role of Risk-Taking Propensity

Individual Differences in Warning Perception: The Role of Risk-Taking Propensity Elizabeth Gabe-Thomas Warnings are intended to improve safety (decreasing injury, illness and loss) by communicating the presence and nature of a potential hazard and encouraging behaviour that will minimise or avoid a negative outcome. Warnings can be seen as representations of risk, therefore it is likely that an individual’s attitude towards risk, their risk-taking propensity, may affect the way they perceive warnings. Establishing this relationship has important practical implications. If high risktaking propensity can predict non-compliance, then attempts may be made to increase compliance within high risk-takers by tailoring warnings to such individuals. This thesis aims to explore empirically the relationship between measures of risk-taking propensity and warnings, with potential application to the prevention of hazardous behaviours. Study One investigated the potential relationship between risk-taking propensity and warning perception using an exploratory approach. The results confirmed that various measures of risktaking propensity predicted warning perceptions, in particular on intentions to comply with the warnings. Studies Two and Three revealed that the relationship between risk-taking and warning perception is domain specific to a certain extent and that it is stronger when contextual information about a hazard is provided. Study Four explored potential underlying mechanisms and revealed that while the mental simulation of positive outcomes of non-compliance was found to be influential, anticipated regret significantly mediated the relationship between risk-taking propensity and intended compliance. Study Five attempted to minimise the discrepancy between high and low risk-takers through warning design manipulation. Despite a strong effect of sensation seeking on intended compliance the warning manipulations implemented had no effect on warning perceptions. This thesis offers a significant contribution to the literature, by establishing empirically the effect of risk propensity on warnings perception and by providing insight into the theoretical underpinnings of this relationship.
Separation of nearly identical repeats in shotgun assemblies using defined nucleotide positions, DNPs

An increasingly important problem in genome sequencing is the failure of the commonly used shotgun assembly programs to correctly assemble repetitive sequences. The assembly of non-repetitive regions or regions containing repeats considerably shorter than the average read length is in practice easy to solve, while longer repeats have been a difficult problem. We here present a statistical method to separate arbitrarily long, almost identical repeats, which makes it possible to correctly assemble complex repetitive sequence regions. The differences between repeat units may be as low as 1% and the sequencing error may be up to ten times higher. The method is based on the realization that a comparison of only a part of all overlapping sequences at a time in a data set does not generate enough information for a conclusive analysis. Our method uses optimal multi-alignments consisting of all the overlaps of each read. This makes it possible to determine defined nucleotide positions, DNPs, which constitute the differences between the repeat units. Differences between repeats are distinguished from sequencing errors using statistical methods, where the probabilities of obtaining certain combinations of candidate DNPs are calculated using the information from the multi-alignments. The use of DNPs and combinations of DNPs will allow for optimal and rapid assemblies of repeated regions. This method can solve repeats that differ in only two positions in a read length, which is the theoretical limit for repeat separation. We predict that this method will be highly useful in shotgun sequencing in the future.
Neurons in Striate Cortex Signal Disparity in Half-Matched Random-Dot Stereograms

Human stereopsis can operate in dense “cyclopean” images containing no monocular objects. This is believed to depend on the computation of binocular correlation by neurons in primary visual cortex (V1). The observation that humans perceive depth in half-matched random-dot stereograms, although these stimuli have no net correlation, has led to the proposition that human depth perception in these stimuli depends on a distinct “matching” computation possibly performed in extrastriate cortex. However, recording from disparity-selective neurons in V1 of fixating monkeys, we found that they are in fact able to signal disparity in half-matched stimuli. We present a simple model that explains these results. This reinstates the view that disparity-selective neurons in V1 provide the initial substrate for perception in dense cyclopean stimuli, and strongly suggests that separate correlation and matching computations are not necessary to explain existing data on mixed correlation stereograms. SIGNIFICANCE STATEMENT The initial step in stereoscopic 3D vision is generally thought to be a correlation-based computation that takes place in striate cortex. Recent research has argued that there must be an additional matching computation involved in extracting stereoscopic depth in random-dot stereograms. This is based on the observation that humans can perceive depth in stimuli with a mean binocular correlation of zero (where a correlation-based mechanism should not signal depth). We show that correlation-based cells in striate cortex do in fact signal depth here because they convert fluctuations in the correlation level into a mean change in the firing rate. Our results reinstate the view that these cells provide a sufficient substrate for the perception of stereoscopic depth.
Differential requirement of MALT1 for BAFF-induced outcomes in B cell subsets

B cell activation factor of the TNF family (BAFF) activates noncanonical nuclear factor κB (NF-κB) heterodimers that promote B cell survival. We show that although MALT1 is largely dispensable for canonical NF-κB signaling downstream of the B cell receptor, the absence of MALT1 results in impaired BAFF-induced phosphorylation of NF-κB2 (p100), p100 degradation, and RelB nuclear translocation in B220+ B cells. This corresponds with impaired survival of MALT1−/− marginal zone (MZ) but not follicular B cells in response to BAFF stimulation in vitro. MALT1−/− MZ B cells also express higher amounts of TRAF3, a known negative regulator of BAFF receptor–mediated signaling, and TRAF3 was found to interact with MALT1. Furthermore, phenotypes associated with overexpression of BAFF, including increased MZ B cell numbers, elevated serum immunoglobulin titers, and spontaneous germinal center formation, were found to be dependent on B cell–intrinsic MALT1 expression. Our results demonstrate a novel role for MALT1 in biological outcomes induced by BAFF-mediated signal transduction.
Researchers’ Needs for Resource Discovery and Collaboration Tools: A Qualitative Investigation of Translational Scientists

Background A critical aspect of clinical and translational science (CTS) is interdisciplinary and collaborative research, which increasingly requires a wide range of computational and human resources. However, few studies have systematically analyzed such resource needs of CTS researchers. Objective To improve our understanding of CTS researchers’ needs for computational and human resources in order to build useful and useable supporting informatics tools. Methods We conducted semistructured interviews of 30 CTS researchers from the University of Michigan, followed by qualitative analysis of the interview transcripts. Results The analysis identified three recurring themes: the need for the federation of information, the need to address information overload, and the need to humanize computing, including strong and well-informed views about the use of social networking tools for research collaboration. These findings helped us to narrow down the available design choices for assisting CTS researchers, and helped to identify potential deficiencies of well-known theoretical frameworks used to guide our study, with suggestions for future remedies. Conclusions The user needs identified through the study, along with concrete design suggestions, provided key design, methodological, and theoretical insights, which are being used to guide the design and development of a CTS resource portal. The results and interview instrument should be useful to other institutions with Clinical and Translational Science Awards that face similar challenges related to helping CTS researchers make more effective use of computational and human resources.
Accountability for the sentencing decision process: Towards a new understanding

Combining the latest work of leading sentencing and punishment scholars from ten different countries, this international volume answers key questions in the study of sentencing and society. It presents not only a rigorous examination of the latest legal and empirical research from around the world, but also reveals the workings of sentencing within society and as a social practice. Traditionally, work in the field of sentencing has been dominated by legal and philosophical approaches. Distinctively, this volume provides a more sociological approach to sentencing: so allowing previously unanswered questions to be addressed and new questions to be opened. This extensive collection is drawn from around one third of the papers presented at the First International Conference on Sentencing and Society. Almost without exception, the chapters have been revised, cross-referenced and updated. The overall themes and findings of the international volume are set out by the opening 'Introduction' and the closing 'Reflections' chapters. Research findings on particular penal policy questions are balanced with an analysis of fundamental conceptual issues, making this international volume suitable reading for: sentencing and punishment scholars, criminal justice policy-makers, and graduate students.
The exercise test as a monitor of disease status in hypokalaemic periodic paralysis

Objective: To evaluate exercise test responses in hypokalaemic periodic paralysis (HPP), to determine its value as a diagnostic tool and the factors that could affect the responses. Methods: 22 subjects were studied from two families with HPP caused by R528H mutation, four patients with thyrotoxic periodic paralysis, 15 normal controls, and four controls with hyperthyroidism. All family members were submitted to clinical evaluation, electrophysiological exercise testing, and DNA analysis. Patients with thyrotoxic periodic paralysis had exercise tests before and after treatment of their hyperthyroidism. Results: Abnormal responses to the exercise tests were obtained only in subjects with recent attacks of weakness. They were not correlated with genotype, as asymptomatic carriers were unaffected. Patients with thyrotoxic periodic paralysis showed pronounced impairment while they were hyperthyroid, but improved when they were euthyroid. One patient with HPP and chronic KCl use had an increase in amplitude potentials over ~20 minutes, possibly related to alteration of potassium homeostasis. Conclusions: The exercise test is a useful diagnostic test for periodic paralysis, but in the absence of recent weakness negative results must be viewed with caution. It has advantages over the DNA test in being a non-invasive functional test that can provide insights into abnormalities of muscle excitability.