Miracle-dz/UniFAR-FTUs huggingface dataset:
A monoclonal antibody to glycoprotein gp85 inhibits fusion but not attachment of Epstein-Barr virus

Epstein-Barr virus (EBV) codes for at least three glycoproteins, gp350, gp220, and gp85. The two largest glycoproteins are thought to be involved in the attachment of the virus to its receptor on B cells, but despite the fact that gp85 induces neutralizing antibody, no function has been attributed to it. As an indirect approach to understanding the role of gp85 in the initiation of infection, we determined the point at which a neutralizing, monoclonal antibody that reacted with the glycoprotein interfered with virus replication. The antibody had no effect on virus binding. To examine the effect of the antibody on later stages of infection, the fusion assay of Hoekstra and colleagues (D. Hoekstra, T. de Boer, K. Klappe, and J. Wilshaut, Biochemistry 23:5675-5681, 1984) was adapted for use with EBV. The virus was labeled with a fluorescent amphiphile that was self-quenched at the high concentration obtained in the virus membrane. When the virus and cell membrane fused, there was a measurable relief of self-quenching that could be monitored kinetically. Labeling had no effect on virus binding or infectivity. The assay could be used to monitor virus fusion with lymphoblastoid lines or normal B cells, and its validity was confirmed by the use of fixed cells and the Molt 4 cell line, which binds but does not internalize the virus. The monoclonal antibody to gp85 that neutralized virus infectivity, but not a second nonneutralizing antibody to the same molecule, inhibited the relief of self-quenching in a dose-dependent manner. This finding suggests that gp85 may play an active role in the fusion of EBV with B-cell membranes.
Ribavirin therapy for Chikungunya arthritis.

BACKGROUND Chikungunya is an acute viral infection presenting with a febrile episode and severe arthralgia, swelling of soft tissues, especially around the ankles. Many patients recover with nonspecific treatment of analgesics. Some patients continue to have subacute crippling arthritis in the legs affecting their mobility. This study was undertaken to see the effect of the antiviral drug Ribavirin in the clinical outcome of these patients. METHODOLOGY Ten patients who continued to have crippling lower limb pains and arthritis for at least two weeks after a febrile episode were taken up for the drug study. Ten similar patients during the same period were included as controls. In the study group Ribavirin was given at 200 mg twice a day for seven days. Both groups were followed up for four weeks. RESULTS All patients in the drug group reported improvement in the joint pains with six of them capable of walking freely. The soft tissue swelling also reduced in eight. In three patients the pain returned after mobilization. Seven patients continued not to receive analgesics after four weeks. CONCLUSIONS Ribavirin may have a direct antiviral property against Chikungunya leading to faster resolution of joint and soft tissue manifestations.
An Analysis of Reading Skills Instruction Provided To Special and General Educators In Their Pre-Service and In-Service Teacher Education

iii ACKNOWLEDGEMENTS. v DEDICATION. ix LIST OF TABLES. xii CHAPTER ONE INTRODUCTION. 1 The Great Debate in Reading. 2 The Components of Reading. 5 Phonemic Awareness. 5 Phonics. 6 Fluency. 6 Vocabulary. 7 Comprehension. 7 The Impact of Reading Problems. 7 General Education Students. 8 Special Education. 8 Reading Instruction in Teacher Education. 9 Pre-Service Teacher Education Programs. 10 In-Service Teacher Education Training. 11 Statement of the Problem. 12 Significance of the Study. 13 Definitions. 15 Limitations. 20 Summary. 21 CHAPTER TWO REVIEW OF RELATED LITERATURE. 22 Reading Research in General Education and Special Education. 23 General Education. 23 Special Education. 63 Reading Instruction in Teacher Education. 97 General Education. 98 Special Education. 115 Summary. 131 CHAPTER THREE METHODOLOGY. 133 Overview. 133
Emotional Modulation of the Late Positive Potential during Picture Free Viewing in Older and Young Adults

Hedonic bias during free viewing of novel emotional and neutral scenes was investigated in older adults and college students. A neurophysiological index of emotional picture processing–the amplitude of the centroparietal late positive potential (LPP)–was recorded from the scalp using a dense sensor array while participants (29 older adults; 21 college students) viewed emotionally engaging or mundane natural scenes that varied in specific content. Both students and older adults showed LPP enhancement when viewing affective, compared to neutral, scenes, and there was no difference in LPP amplitude between older individuals and college students when viewing neutral everyday scenes. However, compared to the college students, older individuals showed attenuated LPP amplitude when viewing emotional scenes, regardless of hedonic valence or specific content. Age related differences could be mediated by a reduction in reactive emotional arousal with age, possible mediated by repeated life exposure to emotional stimuli.
Molecular Cloning and Functional Analysis of the Duck TLR4 Gene

Toll-like receptor 4 (TLR4) recognizes pathogen-associated molecular patterns in some animals and has been shown to be closely associated with several diseases such as tumors, atherosclerosis, and asthma. However, its function in ducks is not clear. Alternative splicing of the TLR4 gene has been identified in pigs, sheep, mice, and other species, but has not yet been reported in the duck. In this study, alternative splicing of the duck TLR4 gene was investigated using reverse transcription-polymerase chain reaction (RT-PCR). Duck TLR4 gene (duTLR4, accession number: KF278109) was found to consist of 3367 nucleotides of coding sequence. An alternative splice form, TLR4-b, was identified and shown by alignment to retain the intron between exons 1 and 2. Real-time quantitative polymerase chain reaction (qPCR) analyses suggested that duTLR4-a (wild-type) mRNA is widely expressed in various healthy tissues, whereas TLR4-b is expressed at only low levels. Following stimulation of normal duck embryo fibroblasts with lipopolysaccharide, the expression of both isoforms initially increased and then decreased. Expression of the wild-type isoform subsequently increased again, while that of the variant remained low. The expression levels of wild-type TLR4 were further analyzed by transient transfection of a pcDNA3.1(+)-TLR4-a overexpression vector into duck embryo fibroblasts. qRT-PCR analyses showed that after stimulation with LPS and poly(I:C) the expression levels of IL-1β, IL6, and MHC II increased with a response-efficacy relationship. Our experimental results indicate that TLR4 plays an important role in resistance to both bacterial and viral infections in the duck.
e-government theories and challenges: findings from a plenary expert panel

In e-government there is an ongoing debate about the theoretical foundations and the typical e-government specific challenges that are encountered. This article aims to provide a better understanding of the interdisciplinary nature of e-government and the challenges that research in e-government encounters. It also aims to determine strategies for creating stronger links to the foundations of the various disciplines and therefore to foster learning from each other. In doing so we present the results of analyzing the discussion of a plenary panel (Evaluating the Multidisciplinary Characteristics of E-Government: Finding the Roots of E-Government) organized during one of the Annual International Conferences on Digital Government Research [17]. The results show the diversity of the topics tackled and theories used by the community. Understanding the context and sociotechnical views is considered as key in a field in which political, public administration, information systems and computer science aspects are blended.
Genetics of performance in the horse.

Criteria used to measure performance, environmental factors that influence performance and estimates of heritability are needed to estimate genetic differences. Published heritability estimates of various measures of performance in the horse are summarized. The average heritability estimates of pulling ability and cutting ability are . 25 and . 04, respectively. Heritability estimates are . 18, . 19 and . 17 for log of earnings from jumping, 3-day event and dressage performance, respectively. Heritability estimates of performance rates, log of earnings, earnings, handicap weight, best handicap weight, time and best time for the Thoroughbred are . 55, . 49, . 09, . 49, . 33, . 15 and . 23, respectively. Heritability estimates of log of earnings, earnings, time and best time for the trotter are . 41, . 20, . 32, and . 25, respectively. The heritability estimate of best time for the pacer is . 23. The effectiveness of selection will depend on which performance trait is to be improved.
A new heterostructure FET

Recently a new FET Structure was rep0rted.l The FET conduction takes place in an inversion channel at a heterojunction interface. For the N-channel FET, the semiconductor active region is p type and an electron inversion channel is created at the heterojunction interface by the presence of a charge sheet of dopant ions (donors in this case) of extreme thinness (~301) and placed in the wideband material just at the heterojunction interface. The charge sheet is totally depleted and is therefore an interface dipole which determines the threshold voltage Vr of the device in exactly the same manner as the Qss charge in a silicon MOSFET. However, in
Long-term outcomes in patients with vestibular schwannomas treated using gamma knife surgery: 10-year follow up.

OBJECT Gamma knife surgery (GKS) has been a safe and effective treatment for vestibular schwannomas in both the short and long term, although less is known about long-term outcomes in the past 10 years. The aim of this study was to clarify long-term outcomes in patients with vestibular schwannomas treated using GKS based on techniques in place in the early 1990s. METHODS Eighty patients harboring a vestibular schwannoma (excluding neurofibromatosis Type 2) were treated using GKS between May 1991 and December 1993. Among these, 73 patients were assessed; seven were lost to follow up. The median duration of follow up was 135 months. The mean patient age at the time of GKS was 56 years old. The mean tumor volume was 6.3 cm3, and the mean maximal and marginal radiation doses applied to the tumor were 28.4 and 14.6 Gy, respectively. Follow-up magnetic resonance images were obtained in 71 patients. Forty-eight patients demonstrated partial tumor remission, 14 had tumors that remained stable, and nine demonstrated tumor enlargement or radiation-induced edema requiring resection. Patients with larger tumors did not fare as well as those with smaller lesions. The actuarial 10-year progression-free survival rate was 87% overall, and 93% in patients with tumor volumes less than 10 cm3. No patient experienced malignant transformation. CONCLUSIONS Gamma knife surgery remained an effective treatment for vestibular schwannomas for longer than 10 years. Although treatment failures usually occurred within 3 years after GKS, it is necessary to continue follow up in patients to reveal delayed tumor recurrence.
High-dose chemotherapy and stem-cell rescue for metastatic germ-cell tumors.

BACKGROUND Metastatic testicular tumors that have not been successfully treated by means of initial chemotherapy are potentially curable with salvage chemotherapy. METHODS We conducted a retrospective review of 184 consecutive patients with metastatic testicular cancer that had progressed after they received cisplatin-containing combination chemotherapy. We gave 173 patients two consecutive courses of high-dose chemotherapy consisting of 700 mg of carboplatin per square meter of body-surface area and 750 mg of etoposide per square meter, each for 3 consecutive days, and each followed by an infusion of autologous peripheral-blood hematopoietic stem cells; the other 11 patients received a single course of this treatment. In 110 patients, cytoreduction with one or two courses of vinblastine plus ifosfamide plus cisplatin preceded the high-dose chemotherapy. RESULTS Of the 184 patients, 116 had complete remission of disease without relapse during a median follow-up of 48 months (range, 14 to 118). Of the 135 patients who received the treatment as second-line therapy, 94 were disease-free during follow-up; 22 of 49 patients who received treatment as third-line or later therapy were disease-free. Of 40 patients with cancer that was refractory to standard-dose platinum, 18 were disease-free. A total of 98 of 144 patients who had platinum-sensitive disease were disease-free, and 26 of 35 patients with seminoma and 90 of 149 patients with nonseminomatous germ-cell tumors were disease-free. Among the 184 patients, there were three drug-related deaths during therapy. Acute leukemia developed in three additional patients after therapy. CONCLUSIONS Testicular tumors are potentially curable by means of high-dose chemotherapy plus hematopoietic stem-cell rescue, even when this regimen is used as third-line or later therapy or in patients with platinum-refractory disease.
A Roadmap to the Programmable World: Software Challenges in the IoT Era

The Internet of Things (IoT) represents the next significant step in the evolution of the Internet and software development. Although most IoT research focuses on data acquisition, analytics, and visualization, a subtler but equally important transition is underway. Hardware advances are making it possible to embed fully fledged virtual machines and dynamic language runtimes virtually everywhere, leading to a Programmable World in which all our everyday things are connected and programmable dynamically. The emergence of millions of remotely programmable devices in our surroundings will pose significant software development challenges. A roadmap from today's cloud-centric, data-centric IoT systems to the Programmable World highlights the technical challenges that deserve to be part of developer education and deserve deeper investigation beyond those IoT topics that receive the most attention today.
Social-psychological determinants of well- and ill-being among vocational dancers : a self-determination theory approach.

Vocational dancers are anecdotally regarded as a group ‘at risk’ of compromised health. Yet little is known of the antecedents of variability in positive and negative indicators of dancers’ welfare. Grounded in the basic needs theory (Deci and Ryan, 2000), a mini-theory of the self-determination framework (Deci & Ryan, 1985, 2000) this thesis examined the social-psychological predictors of indices of well- and ill-being among vocational dancers. In study one, the inter-relationships between dancers’ perceptions of the social environment, basic psychological need satisfaction (BPNS) and reported affective states and exhaustion were explored via structural equation modeling. In study two, changes in autonomy support and BPNS were modeled as predictors of changes in dancers’ burnout during the academic year. Multilevel modeling techniques were employed to examine a) perceptions of autonomy support and BPNS as predictors of dancers’ daily affective states in learning and performance contexts (study three); and b) whether BPNS was relevant to dancers’ cognitive appraisals and hormonal and emotional responses in ‘real life’ performance settings (study four). Overall, this thesis partially supports the tenets of basic needs theory. Findings point to the importance of need supportive environments if elite performers are to experience sustained and optimal physical and psychological health.
DPPro: Differentially Private High-Dimensional Data Release via Random Projection

Releasing representative data sets without compromising the data privacy has attracted increasing attention from the database community in recent years. Differential privacy is an influential privacy framework for data mining and data release without revealing sensitive information. However, existing solutions using differential privacy cannot effectively handle the release of high-dimensional data due to the increasing perturbation errors and computation complexity. To address the deficiency of existing solutions, we propose DPPro, a differentially private algorithm for high-dimensional data release via random projection to maximize utility while guaranteeing privacy. We theoretically prove that DPPro can generate synthetic data set with the similar squared Euclidean distance between high-dimensional vectors while achieving $(\epsilon,\delta)$ -differential privacy. Based on the theoretical analysis, we observed that the utility guarantees of released data depend on the projection dimension and the variance of the noise. Extensive experimental results demonstrate that DPPro substantially outperforms several state-of-the-art solutions in terms of perturbation error and privacy budget on high-dimensional data sets.
Do Step- and Biological Grandparents Show Differences in Investment and Emotional Closeness With Their Grandchildren?

Human children are raised by a variety of caregivers including grandparents. A few studies have assessed potential differences in direct caregiving, financial expenditures, and emotional closeness between biological and step-grandparents. Drawing upon kin selection theory, we hypothesized that step-grandparents would provide less care and be less emotionally close to grandchildren than would biological grandparents. A sample of 341 heterosexual U.S. adults 25–35 years of age in a long-term partnership and with a biological child 5 years of age or younger were recruited via Amazon Mechanical Turk. Subjects provided sociodemographic information and answered questions about the dynamics between their own parent/stepparent and their own youngest biological child (hence, biological/step-grandparenting dynamics). Main analyses were restricted to within-subject comparisons. Results showed that biological grandmothers provided more direct childcare, financial expenditures, and had more emotionally close relationships with grandchildren than did step-grandmothers. Biological grandfathers provided less direct care and had less emotionally close relationships than step-grandfathers but did not exhibit differences in financial expenditures. Biological grandmothers provided more direct care, financial investment, and were more emotionally close to the referential grandchild than were biological grandfathers. Step-grandfathers were more emotionally close and more often played with grandchildren than step-grandmothers. These findings partially support kin selection theory. We discuss the relevance of factors such as competing demands on grandmothers’ investment in biological and step-grandchildren and grandfathering serving in part as mating effort. Sex differences in biological grandparenting also mirror those in parenting. We suggest directions for future research, including on grandfathers, particularly in patrilineal societies.
Artificial Error Generation with Machine Translation and Syntactic Patterns

Shortage of available training data is holding back progress in the area of automated error detection. This paper investigates two alternative methods for artificially generating writing errors, in order to create additional resources. We propose treating error generation as a machine translation task, where grammatically correct text is translated to contain errors. In addition, we explore a system for extracting textual patterns from an annotated corpus, which can then be used to insert errors into grammatically correct sentences. Our experiments show that the inclusion of artificially generated errors significantly improves error detection accuracy on both FCE and CoNLL 2014 datasets.
The role of social skills in the entrepreneurial styles effectuation and causation

This study is about the link between entrepreneurs’ social skills and their styles of entrepreneurship. It is expected that entrepreneurs with high social skills get easier in contact with people they want to use for their benefit, than entrepreneurs with low social skills. Will this lead for the entrepreneur to a focus on selecting the possible effects that can be created with the entrepreneurs’ set of means? Or are social skills not important in processes of causation and effectuation?
A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

The inference of transmission pathways for medicinally important bacteria is important to our understanding of pathogens. Here we report analyses of transmission in Helicobacter pylori, a major carcinogen. Our study is novel in that the focal community comprises detailed family pedigrees and has a high prevalence of H. pylori. To infer transmission, we performed high-resolution analyses of nucleotide sequences for three genes and accounted for the occurrence of mutation and recombination through the use of simulation modeling. Our results demonstrate that transmission has a strong nonfamilial component potentially the result of a large proportion of infections derived from the community. These results are interesting from both a medical and an evolutionary standpoint. First, efficient control measures and beliefs about the sources of H. pylori infection should be reevaluated. Evolutionarily, our results contradict the hypothesis of strict vertical transmission, presented as an explanation for the strong correlation between human population history and H. pylori diversity. Thus the paradox of persistent phylogenetic structure, despite a permissive mode of transmission and high recombination rates, must be solved elsewhere. Here we consider the potential for recombination events to maintain genetic structure in light of horizontal transmission.
Psychometric Properties of a Generic, Patient-Centred Palliative Care Outcome Measure of Symptom Burden for People with Progressive Long Term Neurological Conditions

Background There is no standard palliative care outcome measure for people with progressive long term neurological conditions (LTNC). This study aims to determine the psychometric properties of a new 8-item palliative care outcome scale of symptom burden (IPOS Neuro-S8) in this population. Data and Methods Data were merged from a Phase II palliative care intervention study in multiple sclerosis (MS) and a longitudinal observational study in idiopathic Parkinson’s disease (IPD), multiple system atrophy (MSA) and progressive supranuclear palsy (PSP). The IPOS Neuro-S8 was assessed for its data quality, score distribution, ceiling and floor effects, reliability, factor structure, convergent and discriminant validity, concurrent validity with generic (Palliative care Outcome Scale) and condition specific measures (Multiple Sclerosis Impact Scale; Non-motor Symptoms Questionnaire; Parkinson’s Disease Questionnaire), responsiveness and minimally clinically important difference. Results Of the 134 participants, MS patients had a mean Extended Disability Status Scale score 7.8 (SD = 1.0), patients with an IPD, MSA or PSP were in Hoehn & Yahr stage 3–5. The IPOS Neuro-S8 had high data quality (2% missing), mean score 8 (SD = 5; range 0–32), no ceiling effects, borderline floor effects, good internal consistency (Cronbach’s α = 0.7) and moderate test-retest reliability (intraclass coefficient = 0.6). The results supported a moderately correlated two-factor structure (Pearson’s r = 0.5). It was moderately correlated with generic and condition specific measures (Pearson’s r: 0.5–0.6). There was some evidence for discriminant validity in IPD, MSA and PSP (p = 0.020), and for good responsiveness and longitudinal construct validity. Conclusions IPOS Neuro-S8 shows acceptable to promising psychometric properties in common forms of progressive LTNCs. Future work needs to confirm these findings with larger samples and its usefulness in wider disease groups.