Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcytosis in thyroid follicle cells

Inside-out follicles prepared from pig thyroid glands were used for studies on endocytosis. endocytosis. In this in vitro system, only the apical plasma membranes of follicle cells were exposed to tracers added to the culture medium. Cationized ferritin (CF) bound to the apical plasma membrane and was transferred first to endosomes and to lysosomes (within 5 min). Later, after approximately 30 min, CF was also found in stacked Golgi cisternae. In addition, a small fraction of endocytic vesicles carrying CF particles became inserted into the lateral (at approximately 11 min) and the basal (at approximately 16 min) plasma membranes. Morphometric evaluation of CF adhering to the basolateral cell surfaces showed that the vesicular transport across thyroid follicle cells (transcytosis) was temperature-sensitive; it ceased at 15 degrees C but increased about ninefold in follicles stimulated with thyrotropin (TSH). Thyroglobulin-gold conjugates and [3H]thyroglobulin (synthesized in separate follicle preparations in the presence of [3H]leucine) were absorbed to the apical plasma membrane and detected mainly in lysosomes. A small fraction was also transported to the basolateral cell surfaces where the thyroglobulin preparations detached and accumulated in the newly formed central cavity. As in the case of CF, transcytosis of thyroglobulin depended on the stimulation of follicles with TSH. The observations showed that a transepithelial vesicular transport operates in thyroid follicle cells. This transport is regulated by TSH and includes the transfer of thyroglobulin from the apical to the basolateral plasma membranes. Transcytosis of thyroglobulin could explain the occurrence of intact thyroglobulin in the circulation of man and several mammalian species.
Electron-vibrational coupling in the Fenna-Matthews-Olson complex of Prosthecochloris aestuarii determined by temperature dependent absorption and fluorescence line narrowing measurements

Fluorescence line-narrowing measurements at low temperature were performed on the Fenna−Matthews−Olson complex of Prosthecochloris aestuarii. Superimposed on the phonon wing, several vibronic bands could be observed. By use of these data, the temperature dependence of the lowest-energy absorption band was modeled based on the linear harmonic Franck−Condon approximation. The overall Huang−Rhys factor was estimated to be 0.45. The maximum of the phonon distribution was located at 20 cm-1. Thirty vibrational modes could be observed, and their Franck−Condon factors were estimated. The strongest modes were located at 36, 70, and ∼195 cm-1. For the full width at half-maximum of the inhomogeneous broadening, a value of 80 cm-1 was determined. We did not find any evidence for the presence of different excitonic states in the lowest-energy absorption band.
Inequalities and tail bounds for elementary symmetric polynomial

This paper studies the elementary symmetric polynomials over the reals. We show that if two consecutive elementary symmetric polynomials are small in absolute value, then so are all the elementary symmetric polynomials that follow. We use this to prove probability tail bounds for the symmetric polynomials when the inputs are only t-wise independent, that may be useful in the context of derandomization. We also provide examples of t-wise independent distributions for which our bounds are essentially tight.
Stable chromosomal units determine the spatial and temporal organization of DNA replication

DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication.
TRANSCRIPTION FACTOR BINDING SITE PREDICTION WITH MULTIVARIATE GENE EXPRESSION DATA

Multi-sample microarray experiments have become a standard experimental method for studying biological systems. A frequent goal in such studies is to unravel the regulatory relationships between genes. During the last few years, regression models have been proposed for the de novo discovery of cis-acting regulatory sequences using gene expression data. However, when applied to multi-sample experiments, existing regression based methods model each individual sample separately. To better capture the dynamic relationships in multi-sample microarray experiments, we propose a flexible method for the joint modeling of promoter sequence and multivariate expression data. In higher order eukaryotic genomes expression regulation usually involves combinatorial interaction between several transcription factors. Experiments have shown that spacing between transcription factor binding sites can significantly affect their strength in activating gene expression. We propose an adaptive model building procedure 10 capture such spacing dependent exacting regulatory modules. We apply our methods to the analysis of microarray time-course experiments in yeast and in Arabidopsis. These experiments exhibit very different dynamic temporal relationships. For both data sets, we have found all of the well-known cis-acting regulatory elements in the related context, as well as being able to predict novel elements.
Gone Exercising: Mental Contrasting Promotes Physical Activity among Overweight, Middle-aged, Low-ses Fishermen Predictors of Physical Activity Specified by Health Behavior Theories

Objective: Fantasy realization theory (Oettingen, 2012) proposes that fantasizing about a desired future or dwelling upon negative reality rarely changes behavior whereas mentally contrasting fantasy with reality can be an effective behavior change technique. This is because mental contrasting energizes people to overcome obstacles that stand in the way of their desired future. The present study tested whether mental contrasting promotes rates of physical activity among overweight, middle-aged, and low-SES men. Method: A randomized controlled trial was conducted with members of an angling club in the north of England (N ϭ 467). At baseline, participants completed a postal questionnaire that measured cognitions about physical activity. The intervention was embedded in the questionnaire for relevant participants. Behavior was followed up via telephone at 1 month and 7 months postbaseline. The key outcome measure was a validated, self-report measure of physical activity (Godin, Jobin & Bouillon, 1986) taken at all three time-points. Results: Longitudinal, explanatory, and intention-to-treat analyses each indicated that mental contrasting was effective in enhancing rates of physical activity. Mental contrasting also aided the translation of beliefs about the value and worth of physical activity (instrumental attitudes) into action. Conclusion: Mental contrasting appears to be an effective self-regulatory intervention for promoting physical activity and warrants further tests in health psychology. Regular physical activity is known to reduce mortality and morbidity from many chronic diseases including coronary heart disease, diabetes, colon cancer, hip fractures, high blood pressure, and obesity (e. Physical activity benefits not only indi-viduals' health but also their well-being (U. Rates of physical activity are lower for individuals who are older, have higher BMI scores, and have lower socioeconomic Physical activity rates are especially low among overweight, middle-aged men of low SES (NHS Information Centre, 2006), and it is this group that constitutes the focus of the present research. We report findings from a randomized controlled trial that tested the impact of a promising self-regulation intervention— review by Oettingen, 2012)—in promoting physical activity among overweight, middle-aged, low-SES men. Several health behavior theories (HBTs) have been used to understand rates of physical activity including protection motivation theory (Rogers, 1983), social– cognitive theory (e.g., Bandura, 2001), and the theories of reasoned action and planned behavior (Ajzen, 1991; Ajzen & Fishbein, 1980). Meta-analyses of prospective correlational studies appear to offer support for the variables specified by these theories, with effect sizes in the medium-to-large range (sample-weighed r ϭ. Findings indicate that people's …
Ethanol Modulates Synaptic and Extrasynaptic GABAA Receptors in the Thalamus

Drinking alcohol is associated with the disturbance of normal sleep rhythms, and insomnia is a major factor in alcoholic relapse. The thalamus is a brain structure that plays a pivotal role in sleep regulation and rhythmicity. A number of studies have implicated GABAA receptors (GABAA-Rs) in the anxiolytic, amnestic, sedative, and anesthetic effects of ethanol. In the present study, we examined the effects of ethanol on both synaptic and extrasynaptic GABAA-Rs of relay neurons in the thalamus. We found that ethanol (≥50 mM) elicits a sustained current in thalamocortical relay neurons from the mouse ventrobasal thalamus, and this current is associated with a decrease in neuronal excitability and firing rate in response to depolarization. The steady current induced by ethanol was totally abolished by gabazine and was absent in relay neurons from GABAA-R α4 subunit knockout mice, indicating that the effect of ethanol is to enhance tonic GABA-mediated inhibition. Ethanol (50 mM) enhanced the amplitude of tonic inhibition by nearly 50%. On the other hand, ethanol had no effect on spontaneous or evoked inhibitory postsynaptic currents (IPSCs) at 50 mM but did prolong IPSCs at 100 mM. Ethanol had no effect on the paired-pulse depression ratio, suggesting that the release of GABA from presynaptic terminals is insensitive to ethanol. We conclude that ethanol, at moderate (50 mM) but not low (10 mM) concentrations, can inhibit thalamocortical relay neurons and that this occurs mainly via the actions of ethanol at extrasynaptic GABAA-Rs containing GABAA-R α4 subunits.
CEO Option Compensation , Risk-taking and the Financial Crisis : Evidence from the Banking Industry

This paper examines the relation between CEO option compensation and bank risk-taking, and the role of CEO option compensation in affecting bank performance during the 2007-2008 financial crisis. Through panel regressions, we find that over the sample period (1993-2011), option awards received by bank CEO and CEO option holdings lead to higher bank risk which is not rewarded by better performance. Bank CEOs take more risk by engaging more in financial innovation and maintaining more risky loan portfolios. Institutional investors favor high option compensation in their own interests of pursuing short-term stock price upswing, while a larger board corrects this excessive risk-taking by providing bank CEOs with less option compensation. Cross-sectional evidence shows that during the crisis period, the effect of option compensation in increasing risk-taking and worsening performance comes from exercisable option holdings. In addition to the findings regarding option compensation, stock awards are shown to affect bank risk and performance, while stock holdings play no role.
Network Construction Methods for the Simulation of Stochastic Blockmodels DRAFT

A reader-filler machine adapted to insert a discrete strip of microfilm having a series of image frames into a selected channel of a microfiche jacket. The jacket is provided with parallel channels each having an entry slot adjacent the front end. The machine includes a film drive mechanism which cooperates with an optical viewer. The film strip is advanced by the mechanism along the track of a track block which terminates adjacent the front end of a pivoted table which is spring-loaded. The block includes a pair of pressure fingers projecting from either side of the track exit and a pair of flat locating guides flanking the fingers. In operation, the user first tilts the table downwardly with respect to the track exit and places the jacket thereon to cause the guides to enter the channels on opposite sides of the channel to be loaded, thereby orienting the selected channel with respect to the track exit. The table is then released to cause the pressure fingers to engage the borders of the selected channel and to bring the entry slot of this channel into registration with the track exit. The drive mechanism is then operated to advance the film strip or a section thereof into the selected channel, the frames on the strip being optically viewable to determine the point at which the strip is to be severed.
Low‐molecular‐weight heparins vs. unfractionated heparin in the setting of percutaneous coronary intervention for ST‐elevation myocardial infarction: a meta‐analysis

Summary. Background: The aim of the current study was to perform two separate meta‐analyses of available studies comparing low‐molecular‐weight heparins (LMWHs) vs. unfractionated heparin (UFH) in ST‐elevation myocardial infarction (STEMI) patients treated (i) with primary percutaneous coronary intervention (pPCI) or (ii) with PCI after thrombolysis. Methods: All‐cause mortality was the pre‐specified primary endpoint and major bleeding complications were recorded as the secondary endpoints. Relative risk (RR) with a 95% confidence interval (CI) and absolute risk reduction (ARR) were chosen as the effect measure. Results: Ten studies comprising 16 286 patients were included. The median follow‐up was 2 months for the primary endpoint. Among LMWHs, enoxaparin was the compound most frequently used. In the pPCI group, LMWHs were associated with a reduction in mortality [RR (95% CI) = 0.51 (0.41–0.64), P < 0.001, ARR = 3%] and major bleeding [RR (95% CI) = 0.68 (0.49–0.94), P = 0.02, ARR = 2.0%] as compared with UFH. Conversely, no clear evidence of benefits with LWMHs was observed in the PCI group after thrombolysis. Meta‐regression showed that patients with a higher baseline risk had greater benefits from LMWHs (r = 0.72, P = 0.02). Conclusions: LMWHs were associated with greater efficacy and safety than UFH in STEMI patients treated with pPCI, with a significant relationship between risk profile and clinical benefits. Based on this meta‐analysis, LMWHs may be considered as a preferred anticoagulant among STEMI patients undergoing pPCI.
Genome-Wide Expression Profiling Reveals S100B as Biomarker for Invasive Aspergillosis

Invasive aspergillosis (IA) is a devastating opportunistic infection and its treatment constitutes a considerable burden for the health care system. Immunocompromised patients are at an increased risk for IA, which is mainly caused by the species Aspergillus fumigatus. An early and reliable diagnosis is required to initiate the appropriate antifungal therapy. However, diagnostic sensitivity and accuracy still needs to be improved, which can be achieved at least partly by the definition of new biomarkers. Besides the direct detection of the pathogen by the current diagnostic methods, the analysis of the host response is a promising strategy toward this aim. Following this approach, we sought to identify new biomarkers for IA. For this purpose, we analyzed gene expression profiles of hematological patients and compared profiles of patients suffering from IA with non-IA patients. Based on microarray data, we applied a comprehensive feature selection using a random forest classifier. We identified the transcript coding for the S100 calcium-binding protein B (S100B) as a potential new biomarker for the diagnosis of IA. Considering the expression of this gene, we were able to classify samples from patients with IA with 82.3% sensitivity and 74.6% specificity. Moreover, we validated the expression of S100B in a real-time reverse transcription polymerase chain reaction (RT-PCR) assay and we also found a down-regulation of S100B in A. fumigatus stimulated DCs. An influence on the IL1B and CXCL1 downstream levels was demonstrated by this S100B knockdown. In conclusion, this study covers an effective feature selection revealing a key regulator of the human immune response during IA. S100B may represent an additional diagnostic marker that in combination with the established techniques may improve the accuracy of IA diagnosis.
Biomonitoring for iron, manganese, chromium, aluminum, nickel and cadmium in workers exposed to welding fume: a preliminary study

The control of exposure to welding fumes is increasing importance in promoting a healthy, safe and productive work environment. This study is a case-control design, random study was conducted among welder (56 subjects) and non welder (39 subjects) with more than 1 years experience in the same job task in an automotive parts manufactory within the industrial area at Cikarang in 2013. All subjects were completed physical examination, informed consent and questionnaire. Blood heavy metals were determined by Inductively-Coupled Plasma Mass Spectrometry (ICP-MS). Whole blood iron, manganese, chromium and lead in welder were higher than non-welder, but not different for aluminum, nickel and cadmium. In welder, chromium and manganese correlated with smoking status, cadmium correlated with age and smoking status. In multivariate analysis, wholeblood cadmium correlates with age and smoking status.
On complete gradient shrinking Ricci solitons

In this paper we derive a precise estimate on the growth of potential functions of complete noncompact shrinking solitons. Based on this, we prove that a complete noncompact gradient shrinking Ricci soliton has at most Euclidean volume growth. The latter result can be viewed as an analog of the well-known theorem of Bishop that a complete noncompact Riemannian manifold with nonnegative Ricci curvature has at most Euclidean volume growth.
Optimized reversible BCD adder using new reversible logic gates

Reversible logic has received great attention in the recent years due to their ability to reduce the power dissipation which is the main requirement in low power digital design. It has wide applications advanced computing, low power CMOS design, Optical information processing, DNA computing, bio information, quantum computation and nanotechnology. This paper presents an optimized reversible BCD adder using a new reversible gate. A comparative result is presented which shows that the proposed design is more optimized in terms of number of gates, number of garbage outputs and quantum cost than the existing designs.
Evaluation of Reanalysis Soil Moisture Simulations Using Updated Chinese Soil Moisture Observations

Abstract Using 19 yr of Chinese soil moisture data from 1981 to 1999, the authors evaluate soil moisture in three reanalysis outputs: the 40-yr European Centre for Medium-Range Weather Forecasts (ECMWF) Re-Analysis (ERA-40); the National Centers for Environmental Prediction–National Center for Atmospheric Research (NCEP–NCAR) reanalysis 1 (R-1); and the NCEP–Department of Energy (DOE) reanalysis 2 (R-2) over China. R-2 shows improved interannual variability and better seasonal patterns of soil moisture than R-1 as the result of the incorporation of observed precipitation, but not for all stations. ERA-40 produces a better mean value of soil moisture for most Chinese stations and good interannual variability. Limited observations in the spring indicate a spring soil moisture peak for most of the stations. ERA-40 generally reproduced this event, while R-1 or R-2 generally did not capture this feature, either because the soil was already saturated or the deep soil layer was too thick and damped such a respon.
Summarization Using Reinforcement Learning

We present a new approach to the problem of automatic text summarization called Automatic Summarization using Reinforcement Learning (ASRL) in this paper, which models the process of constructing a summary within the framework of reinforcement learning and attempts to optimize the given score function with the given feature representation of a summary. We demonstrate that the method of reinforcement learning can be adapted to automatic summarization problems naturally and simply, and other summarizing techniques, such as sentence compression, can be easily adapted as actions of the framework. The experimental results indicated ASRL was superior to the best performing method in DUC2004 and comparable to the state of the art ILP-style method, in terms of ROUGE scores. The results also revealed ASRL can search for sub-optimal solutions efficiently under conditions for effectively selecting features and the score function.
Contribution of Work and Family Demands on Job Satisfaction Through Work-Family Conflict

"Objective – Work environment and family roles are two of the most important life domains; they are different but interconnected with each other. Each role requires energy and responsibilities to be handled effectively. Therefore, it is necessary to consider the experience of work-family interaction in this process. The purpose of this study is to develop an understanding of the effect of work and family demands on job satisfaction which is mediated by the experience in work-family interaction, in particular the work-family conflict. Methodology/Technique – Self-report questionnaires were given to permanent employees who work in non-banking financial sectors in Kalimantan Barat, Indonesia. They were selected by purposive sampling and 100 valid questionnaires were collected. Findings – The path analysis results show that work demands have a significant positive effect on work-family conflict, while family demands have no significant effect on work-family conflict, and work-family conflict has a significant negative effect on job satisfaction. Novelty – Although it is believed that work and family demands contribute to outcomes such as job satisfaction, it is unclear how this occurs. It is recommended that organizations need to manage work-related stressors through familyfriendly policies and programs that enable work and family domains to integrate with each other, as well as developing an organizational culture that supports the positive interaction of work and family. "
CHAPTER 10 QUALITY AND NEW TECHNOLOGIES TO CREATE CORN CO-PRODUCTS FROM ETHANOL PRODUCTION

The objective of this chapter is to describe the quality characteristics of distillers dried grains with solubles (DDGS) and new technologies used to produce new fractionated corn co-products. Quality of a feedstuff can be defined in many ways, but as described in Webster’s dictionary, it is the degree of excellence or superiority. Since corn co-products from ethanol production are sources of nutrients, high quality can be described as containing a consistently high level of economically important nutrients (e.g., energy, amino acids, and phosphorus) that are highly digestible for the animal. Another way of describing quality according to Webster’s dictionary is the peculiar and essential character of something. Most corn co-products are produced as a result of the fermentation process used to produce ethanol and contain residual yeast and perhaps other compounds, which may provide additional health and nutritional benefits beyond the nutrients they provide to the animal’s diet.