Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of parathyroid hormone on Wnt signaling pathway in bone

The Wnt signaling pathway has recently been demonstrated to play an important role in bone cell function. In previous studies using DNA microarray analyses, we observed a change in some of the molecular components of the canonical Wnt pathway namely, frizzled‐1 (FZD‐1) and axil, in response to continuous parathyroid hormone (PTH) treatment in rats. In the present study, we further explored other components of the Wnt signaling pathway in rat distal metaphyseal bone in vivo, and rat osteoblastic osteosarcoma cells (UMR 106) in culture. Several Wnt pathway components, including low‐density lipoprotein‐receptor‐related protein 5 (LRP5), LRP6, FZD‐1, Dickkopf‐1 (Dkk‐1), and Kremen‐1 (KRM‐1), were expressed in bone in vivo and in osteoblasts in vitro. Continuous exposure to PTH (1–38) both in vivo and in vitro upregulated the mRNA expression of LRP6 and FZD‐1 and decreased LRP5 and Dkk‐1. These effects in UMR 106 cells were associated with an increase in β‐catenin as measured by Western blots and resulted in functional activation (three to six‐fold) of a downstream Wnt responsive TBE6‐luciferase (TCF/LEF‐binding element) reporter gene. Activation of the TBE6‐luciferase reporter gene by PTH (1–38) in UMR 106 cells was inhibited by the protein kinase A (PKA) inhibitor, H89. Activation was mimicked by PTH (1–31), PTH‐related protein (1–34), and forskolin, but both PTH (3–34) and (7–34) had no effect. These findings suggest that the effect of PTH on the canonical Wnt signaling pathway occurs at least in part via the cAMP‐PKA pathway through the differential regulation of the receptor complex proteins (FZD‐1/LRP5 or LRP6) and the antagonist (Dkk‐1). Taken together, these results reveal a possible role for the Wnt signaling pathway in PTH actions in bone. © 2005 Wiley‐Liss, Inc.
Preliminary study of 1.5-T MR arthrography of the shoulder with 3D isotropic intermediate-weighted turbo spin echo.

OBJECTIVE The purpose of this study was to assess the performance of a near-isotropic 3D turbo spin-echo sequence in comparison with a standard 2D protocol and with arthroscopy in direct 1.5-T MR arthrography of the shoulder. SUBJECTS AND METHODS Dilute gadolinium was injected into three cadaver shoulders, and 3D turbo spin-echo and 2D sequences were evaluated with respect to the signal-to-noise and contrast-to-noise ratios of key tissues. In a prospective study, the 3D intermediate-weighted fat-suppressed sequence (reformatted in three planes) was added to shoulder MR arthrography of 43 consecutively registered patients, 13 of whom later underwent arthroscopy. Two radiologists independently graded the 3D and 2D images in separate sessions to visualize normal anatomic features and to detect pathologic changes in the labrum, cartilage, cuff, and glenohumeral ligaments, assigning confidence levels to their readings. One reader repeated the readings of images of 10 patients. Reports of subsequent arthroscopy were available for 13 patients. RESULTS The sequences performed comparably with respect to signal-to-noise and contrast-to-noise ratios in the cadavers. The 3D images suffered from mildly increased blurring, but the readers were significantly more confident in assessing the proximal biceps tendon and curved portions of the labrum and in their findings of partial tears of the articular side of the supraspinatus tendon and posterior labral tears on the 3D images. A larger number of partial-thickness cartilage defects were found on 2D images. CONCLUSION The 3D turbo spin-echo sequence is a promising technique that can be used in shoulder arthrography with image quality and results comparable to those of traditional 2D techniques. Use of the 3D technique may result in greater anatomic detail in evaluating small obliquely oriented structures, including the curved portions of the labrum and the intraarticular portion of the biceps tendon.
Regulated Degradation of the HIV-1 Vpu Protein through a βTrCP-Independent Pathway Limits the Release of Viral Particles

Viral protein U (Vpu) of HIV-1 has two known functions in replication of the virus: degradation of its cellular receptor CD4 and enhancement of viral particle release. Vpu binds CD4 and simultaneously recruits the βTrCP subunit of the SCFβTrCP ubiquitin ligase complex through its constitutively phosphorylated DS52GXXS56 motif. In this process, Vpu was found to escape degradation, while inhibiting the degradation of βTrCP natural targets such as β-catenin and IκBα. We further addressed this Vpu inhibitory function with respect to the degradation of Emi1 and Cdc25A, two βTrCP substrates involved in cell-cycle progression. In the course of these experiments, we underscored the importance of a novel phosphorylation site in Vpu. We show that, especially in cells arrested in early mitosis, Vpu undergoes phosphorylation of the serine 61 residue, which lies adjacent to the βTrCP-binding motif. This phosphorylation event triggers Vpu degradation by a βTrCP-independent process. Mutation of Vpu S61 in the HIV-1 provirus extends the half-life of the protein and significantly increases the release of HIV-1 particles from HeLa cells. However, the S61 determinant of regulated Vpu turnover is highly conserved within HIV-1 isolates. Altogether, our results highlight a mechanism where differential phosphorylation of Vpu determines its fate as an adaptor or as a substrate of distinct ubiquitin ligases. Conservation of the Vpu degradation determinant, despite its negative effect on virion release, argues for a role in overall HIV-1 fitness.
Factors in the Transfer of Governance-Facilitation Skills within Farmers' Marketing Organizations in Uganda

Abstract Purpose: Training transfer has been examined for formal industrial and service organizations in developed countries but rarely for rural organizations in sub-Saharan Africa. This study sought to identify transfer system factors that best explain the transfer of governance-facilitation skills provided to leaders of farmers' marketing organizations (FMOs) in Uganda. Design/methodology/approach: Face to face interviews using interview schedules containing selected elements of the Learning Transfer System Inventory were conducted with 99 FMO leaders to collect data on the factors that could affect transfer of governance-facilitation skills. Findings: Hierarchical regression revealed that personal capacity to transfer, transfer design, supervisor support and feedback on performance were significant predictors of perceived transfer of governance-facilitation skills. Practical implications: Results point to the need for extension organizations that work with rural farmers' organizations to design effective training methods and transfer approaches that enhance training transfer, as well as promote leadership that values and supports training transfer to FMOs. Originality/value: The focus on member owned, participatorily run rural farmers' organizations to test factors affecting transfer of critical skills adds value to the training transfer discourse. Additionally, the role of trainee characteristics, transfer design and work environment factors in enhancing training transfer within developing country local organizations in rural Africa is confirmed.
A Signaling Perspective on Partner Selection in Venture Capital Syndicates

This paper analyzes the factors impacting partnering decisions in venture capital syndicates using a unique data set of 2,373 venture capitalist (VC) transactions in Germany. We employ a signaling perspective to partner–selection strategies within VC syndicates. By including time–varying information about industry experience and cooperation patterns, we explicitly take into account not only the changing social context for partner selection, but also the dynamic nature of signals sent and received. Our analysis documents that the informativeness of investment experience as a signal depends on the existence and frequency of previous joint deals with the lead VC. Experience becomes a much stronger signal if previous invitations to syndicates are bilateral rather than unilateral. The willingness to invite others to deals signals the ability to reciprocate through one's own deal flow. Moreover, we show how the value of signals erodes over time, that is, information from the previous year carries more informational value than signals from more distant years. In sum, the data reveal that different signals carry weight for lead VCs, and that the frequency of signals sent and the stage of development of the portfolio firm positively moderate the value and relevance of signaling behavior. While early stage investments are mainly characterized by need to diversify and to spread risks, value–added advice is necessary in later rounds, and hence, the strength of the signals sent and received gain in relevance and in value.
A Molecularly Defined Duplication Set for the X Chromosome of Drosophila melanogaster

We describe a molecularly defined duplication kit for the X chromosome of Drosophila melanogaster. A set of 408 overlapping P[acman] BAC clones was used to create small duplications (average length 88 kb) covering the 22-Mb sequenced portion of the chromosome. The BAC clones were inserted into an attP docking site on chromosome 3L using ΦC31 integrase, allowing direct comparison of different transgenes. The insertions complement 92% of the essential and viable mutations and deletions tested, demonstrating that almost all Drosophila genes are compact and that the current annotations of the genome are reasonably accurate. Moreover, almost all genes are tolerated at twice the normal dosage. Finally, we more precisely mapped two regions at which duplications cause diplo-lethality in males. This collection comprises the first molecularly defined duplication set to cover a whole chromosome in a multicellular organism. The work presented removes a long-standing barrier to genetic analysis of the Drosophila X chromosome, will greatly facilitate functional assays of X-linked genes in vivo, and provides a model for functional analyses of entire chromosomes in other species.
“A to P” Screw Versus Posterolateral Plate for Posterior Malleolus Fixation in Trimalleolar Ankle Fractures

Objectives: To compare radiographic and clinical midterm outcomes of posterior malleolar fractures treated with posterior buttress plating versus anterior to posterior lag screw fixation. Design: Retrospective case series. Setting: Level I trauma center. Patients/Participants: Between January 2002 and December 2010, patients with posterior malleolar fractures were identified by Current Procedural Terminology code and their charts reviewed for eligibility. Intervention: Posterior malleolar fixation using either anterior to posterior (AP) lag screws or posterior buttress plating. Main Outcome Measurements: Demographic data, length of follow-up, range of motion, and postoperative Short Musculoskeletal Function Assessment (SMFA) scores were the main outcome measurements. Immediate postoperative radiographs for residual gap/step-off and final follow-up radiographs for the degree of arthritis that developed were evaluated. Results: Thirty-seven patients were eligible for the study, and 27 chose to participate. Sixteen patients underwent posterior buttress plating, and 11 underwent AP screw fixation with mean follow-up times of 54.9 and 32 months, respectively. Demographic data were similar between groups. The posterolateral plating group demonstrated superior postoperative SMFA scores compared with the AP screw group with statistically significant differences in the SMFA bother index (26.7 vs. 9.2, P = 0.03) and trends toward improvement in the mobility (28.3 vs. 12.9, P = 0.08) and functional indices (20.2 vs. 9.4, P = 0.08). There were no significant differences in the range of motion or the development of ankle arthritis over time. Conclusions: Patients with trimalleolar ankle fractures in whom the posterior malleolus was treated with posterolateral buttress plating had superior clinical outcomes at follow-up compared with those treated with AP screws. Level of Evidence: Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
The Impact of Gender on Start-up Capital: A Case of Women Entrepreneurs in South Africa

In South Africa, women entrepreneurs do not enjoy the same opportunities as men in accessing start-up capital due to a number of discriminatory practices embedded in lending models. This study seeks to understand the gender-specific barriers to raising start-up capital, and adopts a mixed methods approach that includes semi-structured interviews with experts and a survey of women entrepreneurs. All participants were from the small business sector in Pietermaritzburg, KwaZulu-Natal, South Africa. The findings revealed the gendered nature of start-up capital for women entrepreneurs in South Africa, and the difficulty women face in venturing into non-traditional industries. The findings build on previous research by focusing on the intersection between women’s contribution to the economy and their production activities in the informal and domestic context. This study calls for the activities of women entrepreneurs in these sectors of the economy to be recognised within lending models, and as constituting a valuable area of economic growth.
Behaviors of Pre‐Venture Entrepreneurs and Perceptions of Their Financial Needs

Some studies have examined different barriers faced by entrepreneurs in starting their firms. For example, lack of business education, training, or managerial experience may have an impact on the firm’s success. This study sought to determine whether there were any differences in the assistance and training needs between male and female pre‐venture entrepreneurs as well as between Hispanic and Anglo pre‐venture entrepreneurs. The training needs focused on the areas of finance and accounting. A sample of 133 clients of a regional Small Business Development Center (SBDC) was used to make this comparison. The results indicated differences between males and females in most cases and especially between Hispanic females and Hispanic males. The Hispanic males felt they needed less assistance in the areas of finance and accounting than the Hispanic females did. However, the SBDC consultants felt that both the Anglo and Hispanic females needed less assistance in those areas than the Anglo and Hispanic males needed.
Gendering of firms’ collaboration networks and innovation: a global study with a focus on China, Japan and South Korea

Innovation in a firm is embedded in a network around the firm, typically, and its networking benefits its innovation. This embeddedness is gendered, we hypothesise, in the way the women's firms tend to network less than men's firms, also when controlling for other things. This micro-level dynamic further differs among societies, notably among China, Japan, South Korea and other countries, we hypothesise. The hypotheses about the world generally, and specifically about mainland China, Japan and South Korea, uses data on 24,937 established firms in 68 countries, from the Global Entrepreneurship Monitor, analysed by hierarchical linear modelling. Findings suggest that networking is more extensive in men's firms. Second, innovation is higher in women's firms. Third, firms' networking benefits innovation similarly much in men's firms and in women's firms. Finally, the gender gap is similarly wide in Japan, South Korea and China, and the countries differ in their firms' networking and innovation.
Human resource management in Europe: evidence from ten countries

The subject of human resource management (HRM) and its development has been much contested in the literature. Most of the relevant theories originated in the United States of America. There is in the literature no distinctly ‘European’ approach to HRM and, indeed, our knowledge of comparative HRM practices in different European states is limited. This paper draws on a major new research project which has gathered data from ten European countries to argue that practices in these countries can be categorized, inter alia, by the degree of integration of HRM into business strategy and the degree of devolvement to line managers. Using these two dimensions gives a quite distinct picture of differences in HRM practices in the ten European countries. These differences are analysed and the validity of the model is discussed. Such a categorization raises the need to consider different conceptual approaches to HRM.
Electron cryo-microscopy of VAT, the archaeal p97/CDC48 homologue from Thermoplasma acidophilum.

VAT (valosine containing protein-like ATPase from Thermoplasma acidophilum), an archaeal member of the AAA-family (ATPases associated with a variety of cellular activities) that possesses foldase as well as unfoldase-activity, forms homo-hexameric rings like its eukaryotic homologues p97 and CDC48. The VAT-monomer exhibits the tripartite domain architecture typical for type II AAA-ATPases: N-D1-D2, whereby N is the substrate binding N-terminal domain preceding domains D1 and D2, both containing AAA-modules. Recent 3-D reconstructions of VAT and p97 as obtained by electron microscopy suffer from weakly represented N-domains, probably a consequence of their flexible linkage to the hexameric core. Here we used electron cryo-microscopy and 3-D reconstruction of single particles in order to generate a 3-D model of VAT at 2.3 nm resolution. The hexameric core of the VAT-complex (diameter 13.2 nm, height 8.4 nm) encloses a central cavity and the substrate-binding N-domains are clearly arranged in the upper periphery. Comparison with the p97 3-D reconstruction and the recently determined crystal structure of p97-N-D1 suggests a tail-to-tail arrangement of D1 and D2 in VAT.
What role does consumer sentiment play in the U.S. macroeconomy

Many in the press and general public see consumer sentiment as a significant, independent force in the economy. Some suggest that sentiment indexes forecast future economic activity, others that changes in consumer sentiment actually drive business cycle fluctuations. ; This article shows that consumer sentiment plays a much more passive role, primarily reflecting rather than causing current economic conditions such as levels of income growth, inflation, unemployment, and interest rates. The author’s statistical tests show that most of the variation in consumer sentiment is explained by these broad macroeconomic variables. The information that is unique to sentiment plays a relatively small role in explaining subsequent variations in consumption expenditures. Similarly, contemporaneous consumer sentiment data have relatively little incremental value in forecasting current activity, beyond what is available in lagged macroeconomic data. Finally, sentiment’s independent role in fluctuations and forecasting has not increased in the 1990s, as some have suggested.
Cross-subsidization, Incentives, and Outcomes in Professional Team Sports Leagues

Professional team sports leagues provide insight into the problems facing the management of functioning cartels. This paper provides an analysis of the incentives and outcomes inherent in the management of professional team sports cartels. Except for revenue sharing and salary caps, league cartel management outcomes are consistent with league-wide revenue maximization and have no impact on competitive balance. However, there are predictable impacts on the profitability of strong- and weak-drawing teams within the league. While providing an analytical review of the literature, the work here also yields new results concerning salary caps, local TV revenue sharing, and the behavior of cartel managers in the face of rival leagues.
Identification of cancer/testis genes by database mining and mRNA expression analysis

Cancer/testis (CT) antigens are immunogenic proteins expressed predominantly in gametogenic tissue and cancer; they are considered promising target molecules for cancer vaccines. The identification of new CT genes is essential to the development of polyvalent cancer vaccines designed to overcome tumor heterogeneity and antigen loss. In the current study, a search for new CT genes was conducted by mining the Unigene database for gene clusters that contain expressed sequence tags derived solely from both normal testis and tumor‐derived cDNA libraries. This search identified 1,325 different cancer/testis‐associated Unigene clusters. The mRNA expression pattern of 73 cancer/testis‐associated Unigene clusters was assessed by reverse transcriptase polymerase chain reaction. Three gene products, CT15/Hs.177959, CT16/Hs.245431 and CT17/Hs.178062, were detected only in testis and in tumor tissue. CT15 is equivalent to ADAM2/fertilin‐β. CT16, an uncharacterized gene product, has homology (30–50%) to members of the GAGE gene family and is 89% identical to CT16.2/Hs.293317, indicating that CT16 and CT16.2 are members of a new GAGE gene family. The uncharacterized gene product, CT17, has homology (30%) to phospholipase A1. RT‐PCR analysis showed that CT15 is expressed exclusively in renal cancer, whereas CT16 and CT17 are expressed in a range of human cancers. Real‐time RT‐PCR analysis of newly defined CT genes and the prototype CT antigens, MAGE‐3 and NY‐ESO‐1, revealed low levels (less than 3% of the level detected in testis) of CT15, CT16 and NY‐ESO‐1 in a limited range of normal, non‐gametogenic tissues. This study demonstrates the merits of database mining with respect to the identification of tissue‐restricted gene products expressed in cancer. © 2002 Wiley‐Liss, Inc.
Impact of Corporate Governance on the Performance of Commercial Banks in Zimbabwe

The background of corporate governance dates back to the 19th Century when state corporation laws enhanced the rights of corporate boards without unanimous consent of shareholders. The concept propounds that corporation should have a good board structure in order to enhance performance. It is firmly rooted on the assumption that good corporate governance practices enhance corporate performance. However, there is no consensus on the impact of corporate governance on performance. The increasing role of the financial sector, on both economic development and poverty alleviation, has seen the concept being applied more on the financial sector than before; this has been further aggravated by world financial crisis, and its consequences. In this regard, Zimbabwe is no exception, during the period 2003-2009 the Zimbabwe witnessed unprecedented failure in the financial sector rooted from a number of issues; but chief among them has been cited as poor corporate governance practices. However, there have been mixed feelings on the extent to which bank performance can be attributed to corporate governance. The paper presents the findings of the study that was conducted to investigate the impact of corporate governance on the performance of commercial banks in Zimbabwe. Using data gathered from 2009-2012, for a sample of five commercial banks, it applies multi-regression model, to assess the causal relationship between corporate governance measures (board size, board composition, internal board committees and board diversity) and bank performance. The results indicate unidirectional causal relationship from corporate governance to bank performance. In addition, there a positive relationship between board composition, board diversity and commercial bank performance, although a negative relationship appears between board size, board committees and bank performance. Therefore, in order to improve performance in commercial banks good corporate governance practices must implemented, this includes improving board structures, disclosure, and fiduciary duties of directors. On the hand the Reserve Bank of Zimbabwe should ensure or put in place robust supervisory and regulatory policies; the development and implementation of a national corporate governance code is long overdue. DOI: 10.5901/mjss.2014. v5n15p93