Miracle-dz/UniFAR-FTUs huggingface dataset:
Preventing the Stress-Induced Shift from Goal-Directed to Habit Action with a β-Adrenergic Antagonist

Stress modulates instrumental action in favor of habit processes that encode the association between a response and preceding stimuli and at the expense of goal-directed processes that learn the association between an action and the motivational value of the outcome. Here, we asked whether this stress-induced shift from goal-directed to habit action is dependent on noradrenergic activation and may therefore be blocked by a β-adrenoceptor antagonist. To this end, healthy men and women were administered a placebo or the β-adrenoceptor antagonist propranolol before they underwent a stress or a control procedure. Shortly after the stress or control procedure, participants were trained in two instrumental actions that led to two distinct food outcomes. After training, one of the food outcomes was selectively devalued by feeding participants to satiety with that food. A subsequent extinction test indicated whether instrumental behavior was goal-directed or habitual. As expected, stress after placebo rendered participants' behavior insensitive to the change in the value of the outcome and thus habitual. After propranolol intake, however, stressed participants behaved, same as controls, goal-directed, suggesting that propranolol blocked the stress-induced bias toward habit behavior. Our findings show that the shift from goal-directed to habitual control of instrumental action under stress necessitates noradrenergic activation and could have important clinical implications, particularly for addictive disorders.
Frequency analysis of EMG signals with Matlab sptool

In the area of biomedical digital signal processing (DSP), wavelet analysis, neural networks and pattern recognition methods are being developed for analysis of EMG signals (generated by the muscles) in neuromuscular disease and CTG (the cardiotocogram) signals during labor. These are traditionally very difficult signals to quantify and innovative approaches to analysis are required for clinical quantification. Software and hardware DSP systems are being designed for real time clinical applications. In this paper, the aim is identification of EMG signals by computing the median and average frequencies and investigating frequency domain behavior of EMG signals. To determine these parameters, fast Fourier transform and digital filters have been very important factors at getting the result.
Odour coding is bilaterally symmetrical in the antennal lobes of honeybees (Apis mellifera)

The primary olfactory neuropil, the antennal lobe (AL) in insects, is organized in glomeruli. Glomerular activity patterns are believed to represent the across‐fibre pattern of the olfactory code. These patterns depend on an organized innervation from the afferent receptor cells, and interconnections of local interneurons. It is unclear how the complex organization of the AL is achieved ontogenetically. In this study, we measured the functional activity patterns elicited by stimulation with odours in the right and the left AL of the same honeybee (Apis mellifera) using optical imaging of the calcium‐sensitive dye calcium green. We show here that these patterns are bilaterally symmetrical (n = 25 bees). This symmetry holds true for all odours tested, irrespective of their role as pheromones or as environmental odours, or whether they were pure substances or complex blends (n = 13 odours). Therefore, we exclude that activity dependent mechanisms local to one AL determine the functional glomerular activity. This identity is genetically predetermined. Alternatively, if activity dependent processes are involved, bilateral connections would have to shape symmetry, or, temporal constraints could lead to identical patterns on both sides due to their common history of odour exposure.
SAIRPS: A generic simulator for evaluation of synthetic aperture interferometric radiometers

ESA's SMOS mission has been a cornerstone in passive Earth Observation, since it has provided for the first time ever with global and frequent observations of Soil Moisture and Ocean Salinity (where the mission acronym comes from). SMOS' single payload is MIRAS: the Microwave Imaging Radiometers by Aperture Synthesis in two dimensions. After SMOS' success, today, a number of instruments are planned or under study: the GeoStar instrument is the baseline payload for the Precipitation and All-weather Temperature and Humidity (PATH) mission from NASA (USA), the Geostationary Atmospheric Sounder (GAS) instrument is under study for post-MSG operational satellites observations (Europe), and the Geostationary Interferometric Microwave Sounder (GIMS) instrument from NSSC-CAS (China). The study of the instrument performance in terms of angular resolution and radiometric performance (radiometric sensitivity and accuracy), and the optimization of this new type of instruments is a complex task that requires dedicated ad-hoc tools. In this work the SAIRPS (Synthetic Aperture Interferometric Radiometer Performance Simulator) is presented. This complex simulator allows to analyze arbitrary receiver topologies, arbitrary array geometries, and includes noise injection calibration algorithms, and new external robust calibration algorithms, and image reconstruction algorithms that allow to evaluate the performance of almost any instrument. At present, the full simulator has been coded in Matlab, translated into C++, and integrated in OpenSF. This work summarizes the key aspects of the architecture of this simulator, completing the presentation made in IGARSS 2012, and presents a few examples of the simulation results using different instrument configurations.
Regulation of male fertility by CFTR and implications in male infertility.

BACKGROUND The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated Cl(-) and HCO(3)(-) conducting channel, mutations of which are known to be associated with male infertility. However, the underlying mechanisms remain elusive. METHODS Literature databases were searched for papers on the topics related to CFTR and male fertility and infertility with relevant keywords. Unpublished data from authors' laboratory were also included for analysis. RESULTS Clinical evidence shows increased mutation frequency or reduced CFTR expression in men with congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities, such as azoospermia teratospermia and oligoasthenospermia. Studies on primary rodent Sertoli cells and germ cells, as well as testes from CFTR knockout mice or a cryptorchidism model, yield findings indicating the involvement of CFTR in spermatogensis through the HCO(3)(-)/sAC/cAMP/CREB(CREM) pathway and the NF-κB/COX-2/PGE(2) pathway. Evidence also reveals a critical role of CFTR in sperm capacitation by directly or indirectly mediating HCO(3)(-) entry that is essential for capacitation. CFTR is emerging as a versatile player with roles in mediating different signaling pathways pertinent to various reproductive processes, in addition to its long-recognized role in electrolyte and fluid transport that regulates the luminal microenvironment of the male reproductive tract. CONCLUSIONS CFTR is a key regulator of male fertility, a defect of which may result in different forms of male infertility other than CBAVD. It would be worthwhile to further investigate the potential of developing novel diagnostic and contraceptive methods targeting CFTR.
Semi-automated estimation of the local flood depth on SAR images

In the context of a flooding, a clear cloud-free SAR (Synthetic Aperture Radar) image proves mainly useful to retrieve flood features that can provide an extensive understanding of the disaster. Among these features, extremely important is the water depth on which this paper will focus by looking for a semi-automated algorithm for its estimation in the neighborhood of a given building from a pair of SAR images. In this study, two SAR images acquired during dry and flooded conditions are necessary, as well as a DSM (Digital Surface Model) to give an a priori knowledge of the height of the building and its footprint. The whole process is divided into two main parts: First, an extraction of the building's double-bounce contribution using Genetic Algorithms, then the computation of the inundated building's height, to eventually evaluate the water level locally in the neighborhood of this building. Thanks to the semi-automation of the double-reflection line retrieval, the execution time of the whole process was reduced from a few minutes (time to manually delineate the double-bounce line) to a few seconds, while keeping an error in the estimated flood depth in the order of a few decimeters (35cm on average).
Compartmentation of brain glutamate metabolism in neurons and glia.

Intrasynaptic [glutamate] must be kept low in order to maximize the signal-to-noise ratio after the release of transmitter glutamate. This is accomplished by rapid uptake of glutamate into astrocytes, which convert glutamate into glutamine. The latter then is released to neurons, which, via mitochondrial glutaminase, form the glutamate that is used for neurotransmission. This pattern of metabolic compartmentation is the "glutamate-glutamine cycle. " This model is subject to the following two important qualifications: 1) brain avidly oxidizes glutamate via aspartate aminotransferase; and 2) because almost no glutamate crosses from blood to brain, it must be synthesized in the central nervous system (CNS). The primary source of glutamate carbon is glucose, and a major source of glutamate nitrogen is the branched-chain amino acids, which are transported rapidly into the CNS. This arrangement accomplishes the following: 1) maintenance of low external [glutamate], thereby maximizing signal-to-noise ratio upon depolarization; 2) the replenishing of the neuronal glutamate pool; 3) the "trafficking" of glutamate through the extracellular fluid in a nonneuroactive form (glutamine); 4) the importation of amino groups from blood, thus maintaining brain nitrogen homeostasis; and 5) the oxidation of glutamate/glutamine, a process that confers an additional level of control in terms of the regulation of brain glutamate, aspartate and gamma-aminobutyric acid.
Gaze vs . Mouse in Games : The Effects on User Experience

The possibilities of eye-tracking technologies in educational gaming are seemingly endless. The question we need to ask is what the effects of gaze-based interaction on user experience, strategy during learning and problem solving are. In this paper we evaluate the effects of two gaze based input techniques and mouse based interaction on user experience and immersion. In a between-subject study we found that although mouse interaction is the easiest and most natural way to interact during problemsolving, gaze-based interaction brings more subjective immersion. The findings provide a support for gaze interaction methods into computer-based educational environments.
PCDD/PCDF: human background data for Germany, a 10-year experience.

This paper gives an overview of the development of the environmental or background exposure of humans to polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in Germany. To determine the background exposure, adipose tissue, human milk, or blood can be used. The good comparability of the matrices analyzed is demonstrated. The daily consumption of low-level contaminated food, mainly of animal origin, leads to the accumulation of PCDDs/PCDFs in the human adipose reservoir. The influence of factors such as various eating habits, severe weight loss, age, and nursing (women only) on the human body burden is discussed. Because of decreasing emission of PCDDs/PCDFs into the environment, a decline of these components in humans could be observed over a time span of 10 years. ImagesFigure 1Figure 2Figure 3Figure 4Figure 5Figure 7Figure 8Figure 9Figure 11
Prevalence of cryptococcal meningitis at a tertiary care centre in Western India (1996-2005).

The incidence of cryptococcal meningitis has increased in recent years, both in HIV-positive and -negative patients (Gottfredsonn, 2000). Among all fungi causing meningitis, Cryptococcus neoformans remains the most common, and the cryptococcal antigen is widely recognized as a valuable diagnostic tool in such cases (Lu et al., 2005; Cunha, 2001). The clinical signs and symptoms of C. neoformans meningitis are indistinguishable from those of many other causes of meningitis. Early diagnosis of cryptococcal infection is therefore necessary for appropriate management. Although cryptococcosis is generally thought to be associated with immunocompromised patients, such as those with acquired immunodeficiency syndrome (AIDS) and various malignancies (Steenbergen & Casadevall, 2000), the incidence among immunocompetent patients has reportedly risen over recent years (Hoang et al., 2004; Shih et al., 2000). This retrospective study reports the incidence of cryptococcal meningitis in both HIV and non-HIV patients at a major tertiary healthcare centre in the metropolitan city of Pune, Western India, over the course of 10 years.
Long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the STREAM study): evidence of safety and efficacy in a 5-year extension study

Objectives: To evaluate the safety and efficacy of 5-year, long-term tocilizumab monotherapy for patients with rheumatoid arthritis. Methods: In an open-label, long-term extension trial following an initial 3-month randomised phase II trial, 143 of the 163 patients who participated in the initial blinded study received tocilizumab monotherapy (8 mg/kg) every 4 weeks. Concomitant therapy with non-steroidal anti-inflammatory drugs and/or oral prednisolone (10 mg daily maximum) was permitted. All patients were evaluated with American College of Rheumatology (ACR) improvement criteria, disease activity score (DAS) in 28 joints, and the European League Against Rheumatism response, as well as for safety issues. Results: 143 patients were enrolled in the open-label, long-term extension trial and 94 (66%) patients had completed 5 years as of March 2007. 32 patients (22%) withdrew from the study due to adverse events and one patient (0.7%) due to unsatisfactory response. 14 patients withdrew because of the patient’s request or other reasons. The serious adverse event rate was 27.5 events per 100 patient-years, with 5.7 serious infections per 100 patient-years, based on a total tocilizumab exposure of 612 patient-years. Of the 88 patients receiving corticosteroids at baseline, 78 (88.6%) were able to decrease their corticosteroid dose and 28 (31.8%) discontinued corticosteroids. At 5 years, 79/94 (84.0%), 65/94 (69.1%) and 41/94 (43.6%) of the patients achieved ACR20, ACR50, and ACR70 improvement criteria, respectively. Remission defined as DAS28 less than 2.6 was achieved in 52/94 (55.3%) of the patients. Conclusion: In this 5-year extension study, tocilizumab demonstrated sustained long-term efficacy and a generally good safety profile.
Membrane Disruption by Antimicrobial Fatty Acids Releases Low-Molecular-Weight Proteins from Staphylococcus aureus

ABSTRACT The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward Gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1Δ9) were nontoxic, whereas palmitoleate (16:1Δ9) was a potent growth inhibitor. Cells treated with 16:1Δ9 exhibited rapid membrane depolarization, the disruption of all major branches of macromolecular synthesis, and the release of solutes and low-molecular-weight proteins into the medium. Other cytotoxic lipids, such as glycerol ethers, sphingosine, and acyl-amines blocked growth by the same mechanisms. Nontoxic 18:1Δ9 was used for phospholipid synthesis, whereas toxic 16:1Δ9 was not and required elongation to 18:1Δ11 prior to incorporation. However, blocking fatty acid metabolism using inhibitors to prevent acyl-acyl carrier protein formation or glycerol-phosphate acyltransferase activity did not increase the toxicity of 18:1Δ9, indicating that inefficient metabolism did not play a determinant role in fatty acid toxicity. Nontoxic 18:1Δ9 was as toxic as 16:1Δ9 in a strain lacking wall teichoic acids and led to growth arrest and enhanced release of intracellular contents. Thus, wall teichoic acids contribute to the structure-specific antimicrobial effects of unsaturated fatty acids. The ability of poorly metabolized 16:1 isomers to penetrate the cell wall defenses is a weakness that has been exploited by the innate immune system to combat S. aureus.
Methanol maser polarization in W3(OH)

We present the first 6.7-GHz methanol maser linear polarization map of the extended filamentary maser structure around the compact H ii region W3(OH). The methanol masers show linear polarization of up to ∼8 per cent and the polarization angles indicate a magnetic field direction along the north–south maser structure. The polarization angles are consistent with those measured for the OH masers, taking into account external Faraday rotation toward W3(OH), and confirm that the OH and methanol masers are found in similar physical conditions. Additionally we discuss the Zeeman splitting of the 6.7-GHz methanol transition and present an upper limit of ∼22 mG for the magnetic field strength in the maser region. The upper limit is fully consistent with the field strengths derived from OH maser Zeeman splitting.
Extrinsic extracellular DNA leads to biofilm formation and colocalizes with matrix polysaccharides in the human pathogenic fungus Aspergillus fumigatus

The environmentally acquired fungal pathogen Aspergillus fumigatus causes a variety of severe diseases. Furthermore, it is often found colonizing the respiratory tract of patients suffering from cystic fibrosis. Conidia of this filamentous fungus adhere to substrate surfaces and germinate to form biofilms comprised of dense hyphal networks embedded in an adhesive extracellular matrix (ECM), built predominantly of polysaccharides. These fungal microconsortia are likely to be of clinical relevance, as they have also been observed during growth in the host and they confer drastically reduced susceptibility to antifungals. Little is known about environmental factors or signals contributing to the formation and structural organization of this polysaccharide matrix. Extracellular DNA (eDNA) is an abundant molecule in the mucus-rich surfaces in the lungs of cystic fibrosis patients. Here, we studied its influence on the biofilm establishment and progression of A. fumigatus. Using an in vitro biofilm model eDNA was identified as an efficient biofilm inducer promoting conidial surface adhesion and polysaccharide ECM production. Confocal laser scanning microscopy revealed entirely different ECM architectures depending on the substrates used for biofilm induction. In the presence of serum, adhesive polysaccharides were mainly localized to the hyphal tips appearing as cohesive threads or “halo” areas agglutinating the hyphae. Exogenous DNA altered the structural organization of the biofilm specifically by colocalizing to a grid-like bottom layer of ECM. These results indicate that biofilm formation in A. fumigatus is shaped by certain substrates and in response to host environmental signals.
Barriers to treatment of failed or interferon ineligible patients in the era of DAA: single center study

Background/Aims Interferon-based treatment is not appropriate for a large number of patients with chronic hepatitis C for various medical and social reasons. Newly developed directly acting antivirals (DAAs) have been used to treat chronic hepatitis C without severe adverse effects and have achieved a sustained viral response (SVR) rate of 80-90% with short treatment duration. We were interested to determine whether all patients who failed to respond to or were ineligible for interferon-based therapy could be treated with DAAs. Methods Medical records of patients with positive serum anti-hepatitis C virus (HCV) or HCV RNA between January 2009 and December 2013 were reviewed. Demographic, clinical, and treatment data were collected for analysis. Results A total of 876 patients were positive for both anti-HCV and HCV RNA. Of these, 244 patients were eligible for interferon, although this was associated with relapse in 39 (16%) of patients. In total, 130 patients stopped interferon therapy (67% adverse effects, 28% non-adherent, 4% malignancy, 1% alcohol abuse) and 502 patients were ineligible (66% medical contraindications, 25% non-adherent, 5% socioeconomic problems). Among 671 patients who were ineligible for or failed to respond to interferon therapy, more than 186 (27.7%) could not be treated with DAA due to financial, social, or cancer-related conditions. Conclusions Newly developed DAAs are a promising treatment for patients with chronic hepatitis C who are ineligible for or failed to respond to interferon-based therapy. Nevertheless, not all chronic hepatitis C patients can be treated with DAAs due to various reasons.
Effects of atrial defibrillation shocks on the ventricles in isolated sheep hearts.

BACKGROUND The effects of cardioversion of atrial fibrillation on the activation sequence of the ventricles have not been previously studied. In this study we examined the events in the ventricle that follow the application of atrial defibrillatory shocks. METHODS AND RESULTS We used video imaging technology to study the sequence of activation on the surface of the ventricles in the Langendorff-perfused sheep heart. We recorded transmembrane potentials simultaneously from over 20000 sites on the epicardium before and after biphasic shocks applied by a programmable atrial defibrillator. The first epicardial activation after the shock depended on both the voltage and timing of the shock. During ventricular diastole shocks as low as 10 V produced ventricular excitation, although the time between the shock and the first epicardial activation (latency) was approximately 30 ms. As the shock voltage was increased to 120 V, latency decreased to zero and the entire epicardium was depolarized within 30 ms. For 120-V shocks delivered late in systole, the depolarization sequence produced by the shock was similar to the previous repolarization sequence. Shocks of 120 V applied 150 to 300 ms after the previous ventricular excitation induced ventricular fibrillation. Ventricular fibrillation was induced by multiple focal beats after the shock, which produced waves that propagated but broke down into reentry within regions of high repolarization gradients. CONCLUSIONS These results demonstrate that atrial defibrillation shocks excite the ventricles even at low shock voltages. In addition, ventricular fibrillation can be induced by shocks given in the vulnerable period by producing focal patterns that break down into reentrant waves.