Miracle-dz/UniFAR-FTUs huggingface dataset:
Respiratory Carbon Metabolism following Illumination in Intact French Bean Leaves Using 13C/12C Isotope Labeling1

The origin of the carbon atoms in the CO2 respired by French bean (Phaseolus vulgaris) leaves in the dark has been studied using 13C/12C isotopes as tracers. The stable isotope labeling was achieved through a technical device that uses an open gas-exchange system coupled online to an elemental analyzer and linked to an isotope ratio mass spectrometer. The isotopic analysis of the CO2 respired in the dark after a light period revealed that the CO2 was labeled, but the labeling level decreased progressively as the dark period increased. The pattern of disappearance depended on the amount of carbon fixed during the labeling and indicated that there were several pools of respiratory metabolites with distinct turnover rates. We demonstrate that the carbon recently assimilated during photosynthesis accounts for less than 50% of the carbon in the CO2 lost by dark respiration and that the proportion is not influenced by leaf starvation in darkness before the labeling. Therefore, most of the carbon released by dark respiration after illumination does not come from new photosynthates.
MUC 1 gene polymorphism rs 4072037 and susceptibility to gastric cancer : a meta-analysis

The association between MUC1 polymorphism rs4072037 and the risk of gastric cancer has been described in several studies. However, these studies yielded inconsistent results, especially in different pathological type of gastric cancer. Therefore, we performed this meta-analysis to evaluate the relationship between MUC1 gene polymorphism and gastric cancer susceptibility. A comprehensive database search was performed to identify eligible studies. Odds ratios with 95% confidence intervals were calculated to assess the strength of the association between MUC1 rs4072037 and risk of gastric cancer. Subgroup analyses, publication bias, and sensitivity analyses were also conducted. PubMed, EMBASE, Web of Science and CNKI databases were systematically searched to identify relevant studies. A total of 9 studies (12 datasets) were included in the meta-analysis including 10,410 cases and 11,437 controls. Overall, the G allele at rs4072037 of MUC1 gene was associated with a significant decreased gastric cancer risk (OR=0.70, 95% CI: 0.64–0.76). The association was significant in both anatomic location and pathological subtype subgroup analyses. However, the association was detected in Asian rather than Caucasian. Our findings demonstrate that the presence of the G allele at rs4072037 of the MUC1 gene may contribute to protection against gastric cancer in Asian. Further large studies of multiethnic groups are needed to validate these findings.
Is papillary thyroid microcarcinoma an indolent tumor?

AbstractThe increasing detection of papillary thyroid microcarcinoma (PTMC) has created management dilemmas. To clarify the clinical significance of postsurgery stimulated thyroglobulin (ps-Tg) in PTMC who undergo thyroidectomy and radioactive iodine (RAI), we retrospectively reviewed the 358 PTMC patients who were treated with RAI and followed up in our hospital. Those with an excessive anti-Tg antibody, ultrasound-detected residual were excluded, thereby resulting in the inclusion of 280 cases. Their clinical and histopathological information and clinical outcomes were collected and summarized. Tumor stages were classified according to the tumor, node, metastasis (TNM) staging system and the consensus of the European Thyroid Association (ETA) risk stratification system, respectively. Kaplan–Meier curves were constructed to compare the disease-free survival (DFS) rates of different risk-staging systems. By the end of follow-up, none of the patients died of the disease or relapsed. The 8-year DFS rate was 76.9%. Kaplan–Meier curves showed different DFS rates in TNM stages I versus IV, III versus IV, very low risk versus high risk, low risk versus high risk, respectively (P < 0.05), while they were not significantly different in stage I versus stage III, very low risk versus low risk (P > 0.05). Finally, 40 (14.3%) cases got a persistent disease. Five variables (male sex, nonconcurrent benign pathology, initial tumor size >5 mm, lymph node metastasis, and ps-Tg ≥ 10 &mgr;g/L) were associated with disease persistence by univariate regression analysis. Ps-Tg ≥ 10 &mgr;g/L was the only independent prognostic variable that predicted disease persistence by multivariate regression analysis (odds ratio: 36.057, P = 0.000). Therefore, PTMC with a small size of ⩽1 cm does not always act as an indolent tumor. In conclusion, ps-Tg ≥ 10 &mgr;g/L is associated with increased odds of disease persistence. ETA risk stratification is more effective in predicting disease persistence than the TNM classification system.
Period problems: disorders of menstruation in adolescents

Adolescence is a time of great psychological and physical change. In the UK, girls enter puberty around the age of 10 years with a median age of menarche of 12.9 years; thereafter, it may be several years before regular menstrual cycles are established. Variations in the type and the frequency of periods may create anxiety regarding ill health or serious underlying disorders. With the increase in childhood obesity and subsequent polycystic ovary syndrome, there is a greater awareness and presentation of girls with disorders of menstruation. This review focuses on normal variations of menses and common pathological causes of menstrual problems, including amenorrhoea, dysmenorrhoea and menorrhagia. Further consideration is given to the variations of presentation of polycystic ovary syndrome. It provides a guide to evaluate the various symptoms, investigations and management options.
Polymorphism in the Androgen Receptor and Mammographic Density in Women Taking and Not Taking Estrogen and Progestin Therapy

There is some evidence that women with a higher number of CAG repeat lengths on the androgen receptor (AR) gene have increased breast cancer risk. We evaluated the association between AR-CAG repeat length and mammographic density, a strong breast cancer risk factor, in 404 African-American and Caucasian breast cancer patients. In postmenopausal estrogen progestin therapy users, carriers of the less active AR-CAG had statistically significantly higher mean percentage of density (41.4%) than carriers of the more active AR-CAG (25.7%; P = 0.04). Our results raise the question of whether the number of AR-CAG repeats predicts breast cancer risk in estrogen progestin therapy users.
Colletotrichum higginsianum Mitogen-Activated Protein Kinase ChMK1: Role in Growth, Cell Wall Integrity, Colony Melanization, and Pathogenicity

Colletotrichum higginsianum is an economically important pathogen that causes anthracnose disease in a wide range of cruciferous crops. To facilitate the efficient control of anthracnose disease, it will be important to understand the mechanism by which the cruciferous crops and C. higginsianum interact. A key step in understanding this interaction is characterizing the mitogen-activated protein kinases (MAPK) signaling pathway of C. higginsianum. MAPK plays important roles in diverse physiological processes of multiple pathogens. In this study, a Fus3/Kss1-related MAPK gene, ChMK1, from C. higginsianum was analyzed. The results showed that the Fus3/Kss1-related MAPK ChMK1 plays a significant role in cell wall integrity. Targeted deletion of ChMK1 resulted in a hypersensitivity to cell wall inhibitors, reduced conidiation and albinistic colonies. Further, the deletion mutant was also unable to form melanized appressorium, a specialized infection structure that is necessary for successful infection. Therefore, the deletion mutant loses pathogenicity on A. thaliana leaves, demonstrating that ChMK1 plays an essential role in the early infection step. In addition, the ChMK1 deletion mutant showed an attenuated growth rate that is different from that of its homolog in Colletotrichum lagenarium, indicating the diverse roles that Fus3/Kss1-related MAPKs plays in phytopathogenic fungi. Furthermore, the expression level of three melanin synthesis associated genes were clearly decreased in the albinistic ChMK1 mutant compared to that of the wild type strain, suggesting that ChMK1 is also required for colony melanization in C. higginsianum.
Mumps in the Czech Republic in 2013: Clinical Characteristics, Mumps Virus Genotyping, and Epidemiological Links.

AIM The aim of the study was to map the incidence of mumps in the Czech Republic in terms of clinical symptoms, epidemiological links, and characteristics of circulating genotypes. METHODS Patients with suspected mumps examined in the Infectious Diseases Clinic of the Na Bulovce Hospital in 2013 were enrolled in the study. Buccal swab specimens were tested by means of nucleic acid detection (RT-qPCR) and when positive, they were cultured in tissue culture. Sequencing was carried out using the BigDye Terminator v3.1 Cycle Sequencing Kit and Genetic Analyzer 3500. The SeqScape software was used for the analysis of sequencing data and filtering out low quality reads. The phylogenetic analysis and genotyping were performed using the Mega 6 software. To generate the phylogenetic tree, all sequences were aligned by the MAFFT tool and the alignment obtained was edited using the BioEdit software. In all patients, selected biochemical markers (C-reactive protein, white blood cell count and serum amylase) were measured. The EPIDAT system used for reporting infectious diseases, record keeping, and data analysis in the Czech Republic was the source of statistical data. RESULTS Eighty-nine patients with suspected mumps were examined in the Na Bulovce Hospital and 65 of them were laboratory confirmed with mumps: 40 males (61.5%) and 25 females (38.5%). The mean age of the study cohort was 25.9 years (median age of 23 years, age range from 10 to 73 years) and 14 patients were under 18 years of age. Thirty-four (52.3%) patients were vaccinated in childhood, 28 (43.1%) were unvaccinated, and for three persons, vaccination data were not available. A severe course of the disease was reported in 15 (23.1%) patients. Fourteen of them needed hospitalization because of orchitis (9 males) and meningitis (5 patients). One patient with orchitis was treated on an outpatient basis. The need for hospitalization tended to be lower in the unvaccinated patients (14.7% vs. 35.7%, p=0.076). In 2013, 1,553 cases of mumps were reported to the EPIDAT system. Of these, 640 were laboratory confirmed. The most often reported complications were orchitis (90 cases, i.e. 10.3%) and meningitis (21 cases, i.e. 1.4%). Orchitis was diagnosed in 30.3% of the unvaccinated and in 6.4% of the vaccinated males. Meningitis occurred in 3.1% of the unvaccinated and in 1.0% of the vaccinated patients. CONCLUSION Despite the emergence of mumps among the vaccinated population, the present study has confirmed a positive effect of the vaccine, particularly on the incidence of complications and inflammatory markers. All 30 sequenced mumps virus strains were assigned to group G. A secondary vaccine failure due to waning immunity seems to be a plausible explanation for the rise in mumps cases.
The Role of Personal Computers in Undergraduate Education

This scoping study investigated the role that personal computers play in supporting undergraduate study practice. Monitoring software was installed on the personal computers of five third year students undertaking a summer school course at the University of Otago in 2012. The data showed students' daily academic use of their personal computers was significantly lower compared with their non-academic use. There was also no obvious relationship found when comparing overall computer use with course assignment schedules. In addition, students' perceived use of personal computers as captured through self-reports was found to be contrary to their actual use as recorded by the computer monitoring software. The low level of use for academic purposes indicates that the personal computers did not play a significant role in the daily study practices of these students and that the current perceptions that such devices are vital to student learning may be unsound.
Exploring the Thalamus and Its Role in Cortical Function

The thalamus plays a critical role in perceptual processing, but many questions remain about what thalamic activities contribute to sensory and motor functions. In this book, two pioneers in research on the thalamus examine the close two-way relationships between thalamus and cerebral cortex and look at the distinctive functions of the links between the thalamus and the rest of the brain. Countering the dominant "corticocentric" approach to understanding the cerebral cortex -- which does not recognize that all neocortical areas receive important inputs from the thalamus and send outputs to lower motor centers -- S. Murray Sherman and R.W. Guillery argue for a reappraisal of the way we think about the cortex and its interactions with the rest of the brain. The book defines some of the functional categories critical to understanding thalamic functions, including the distinctions between drivers (pathways that carry messages to the cortex) and modulators (which can change the pattern of transmission) and between first-order and higher-order thalamic relays -- the former receiving ascending drivers and the latter receiving cortical drivers. This second edition further develops these distinctions with expanded emphasis throughout the book on the role of the thalamus in cortical function. An important new chapter suggests a structural basis for linking perception and action, supplying supporting evidence for a link often overlooked in current views of perceptual processing.
Tocotrienol supplementation in postmenopausal osteoporosis: evidence from a laboratory study

OBJECTIVE: Accelerated bone loss that occurs in postmenopausal women has been linked to oxidative stress and increased free radicals. We propose the use of antioxidants to prevent and reverse postmenopausal osteoporosis. This study aimed to examine the effects of tocotrienol, a vitamin E analog, on bone loss due to estrogen deficiency. Our previous study showed that tocotrienol increased the trabecular bone volume and trabecular number in ovariectomized rats. In the current study, we investigated the effects of tocotrienol supplementation on various biochemical parameters in a postmenopausal osteoporosis rat model. MATERIALS AND METHODS: A total of 32 female Wistar rats were randomly divided into four groups. The baseline group was sacrificed at the start of the study, and another group was sham operated. The remaining rats were ovariectomized and either given olive oil as a vehicle or treated with tocotrienol at a dose of 60 mg/kg body weight. After four weeks of treatment, blood was withdrawn for the measurement of interleukin-1 (IL1) and interleukin-6 (IL6) (bone resorbing cytokines), serum osteocalcin (a bone formation marker) and pyridinoline (a bone resorption marker). RESULTS: Tocotrienol supplementation in ovariectomized rats significantly reduced the levels of osteocalcin, IL1 and IL6. However, it did not alter the serum pyridinoline level. CONCLUSION: Tocotrienol prevented osteoporotic bone loss by reducing the high bone turnover rate associated with estrogen deficiency. Therefore, tocotrienol has the potential to be used as an anti-osteoporotic agent in postmenopausal women.
Responses of aortic depressor nerve‐evoked neurones in rat nucleus of the solitary tract to changes in blood pressure

1 Using electrophysiological techniques, the discharge of neurones in the nucleus of the solitary tract (NTS) receiving aortic depressor nerve (ADN) inputs was examined during blood pressure changes induced by I.V. phenylephrine or nitroprusside in anaesthetized, paralysed and artificially ventilated rats. 2 Various changes in discharge rate were observed during phenylephrine‐induced blood pressure elevations: an increase (n= 38), a decrease (n= 5), an increase followed by a decrease (n= 4) and no response (n= 11). In cells receiving a monosynaptic ADN input (MSNs), the peak discharge frequency response was correlated to the rate of increase in mean arterial pressure (P < 0.01) but was not correlated to the absolute increase in blood pressure. The peak discharge frequency response of cells receiving a polysynaptic ADN input (PSNs) was correlated to neither the absolute increase in blood pressure nor the rate of increase in mean arterial pressure. 3 Diverse changes in discharge rate were observed during nitroprusside‐induced reductions in blood pressure: an increase (n= 3), a decrease (n= 10), an increase followed by a decrease (n= 3) and no response (n= 6). Reductions in pressure of 64 ± 2 mmHg produced weak reductions in spontaneous discharge of 1.3 ± 0.9 Hz and only totally abolished spontaneous discharge in one neurone. 4 These response patterns of NTS neurones during changes in arterial pressure suggest that baroreceptor inputs are integrated differently in MSNs compared to PSNs. The sensitivity of MSNs to the rate of change of pressure provides a mechanism for the rapid regulation of cardiovascular function. The lack of sensitivity to the mean level of a pressure increase in both MSNs and PSNs suggests that steady‐state changes in pressure are encoded by the number of active neurones and not graded changes in the discharge of individual neurones. Both MSNs and PSNs receive tonic excitatory inputs from the arterial baroreceptors; however, these tonic inputs appear to be insufficient to totally account for their spontaneous discharge.
Real-Time Urban Monitoring Using Cell Phones: A Case Study in Rome

This paper describes a new real-time urban monitoring system. The system uses the Localizing and Handling Network Event Systems (LocHNESs) platform developed by Telecom Italia for the real-time evaluation of urban dynamics based on the anonymous monitoring of mobile cellular networks. In addition, data are supplemented based on the instantaneous positioning of buses and taxis to provide information about urban mobility in real time, ranging from traffic conditions to the movements of pedestrians throughout the city. This system was exhibited at the Tenth International Architecture Exhibition of the Venice Biennale. It marks the unprecedented monitoring of a large urban area, which covered most of the city of Rome, in real time using a variety of sensing systems and will hopefully open the way to a new paradigm of understanding and optimizing urban dynamics.
Accurate odometry and error modelling for a mobile robot

This paper presents the key steps involved in the design, calibration and error modelling of a low cost odometry system capable of achieving high accuracy dead-reckoning. A consistent error model for estimating position and orientation errors has been developed. Previous work on propagating odometry error covariance relies on incrementally updating the covariance matrix in small time steps. The approach taken here sums the noise theoretically over the entire path length to produce simple closed form expressions, allowing efficient covariance matrix updating after the completion of path segments. Systematic errors due to wheel radius and wheel base measurement were first calibrated with UMBmark test. Experimental results show that, despite its low cost, our system's performance, with regard to dead-reckoning accuracy, is comparable to some of the best reported odometry vehicle.
Anicteric viral hepatitis. A clinicopathological follow-up study in Taiwan.

ANICTERIC viral hepatitis is a widely discussed but little studied entity. Its existence has been acknowledged for many years on the basis of epidemiologic and clinical data, mostly indirect and inferential. For example, during epidemics when the hepatitis infection rate is high, cases in which clinical evidence of hepatitis develops without jaundice are often diagnosed as anicteric hepatitis.1 2 3 In addition, clinicopathological studies have been done on patients in whom chronic liver disease developed without jaundice or with the late appearance of jaundice; the diagnosis of anicteric hepatitis was made retrospectively.4 5 6 The military population of Taiwan offers advantages to the study .
Adapalene inhibits the activity of cyclin-dependent kinase 2 in colorectal carcinoma

Cyclin-dependent kinase 2 (CDK2) has been reported to be overexpressed in human colorectal cancer; it is responsible for the G1-to-S-phase transition in the cell cycle and its deregulation is a hallmark of cancer. The present study was the first to use idock, a free and open-source protein-ligand docking software developed by our group, to identify potential CDK2 inhibitors from 4,311 US Food and Drug Administration-approved small molecular drugs with a re-purposing strategy. Among the top compounds identified by idock score, nine were selected for further study. Among them, adapalene (ADA; CD271,6-[3-(1-adamantyl)-4-methoxyphenyl]-2-naphtoic acid) exhibited the highest anti-proliferative effects in LoVo and DLD1 human colon cancer cell lines. Consistent with the expected properties of CDK2 inhibitors, the present study demonstrated that ADA significantly increased the G1-phase population and decreased the expression of CDK2, cyclin E and retinoblastoma protein (Rb), as well as the phosphorylation of CDK2 (on Thr-160) and Rb (on Ser-795). Furthermore, the anti-cancer effects of ADA were examined in vivo on xenograft tumors derived from DLD1 human colorectal cancer cells subcutaneously inoculated in BALB/C nude mice. ADA (20 mg/kg orally) exhibited marked anti-tumor activity, comparable to that of oxaliplatin (40 mg/kg), and dose-dependently inhibited tumor growth (P<0.05), while combined administration of ADA and oxaliplatin produced the highest therapeutic effect. To the best of our knowledge, the present study was the first to indicate that ADA inhibits CDK2 and is a potential candidate drug for the treatment of human colorectal cancer.
Faktor-faktor Yang Mempengaruhi Remaja Terlibat Dalam Masalah Sosial Di Sekolah Tunas Bakti, Sungai Lereh, Melaka.

Kajian ini bertujuan untuk mengenalpasti faktor-faktor yang mempengaruhi remaja terlibat dalam masalah sosial di Sekolah Tunas Bakti, Sungai Lereh yang terletak di negeri Melaka. Seramai 86 orang pelajar dipilih sebagai sampel kajian. Kajian ini menggunakan satu set soal selidik sebagai instrumen kajian yang mengandungi dua bahagian iaitu Bahagian A mengenai demografi responden dan Bahagian B mengenai respon responden terhadap aspek persoalan kajian. Terdapat empat persoalan kajian yang diutarakan dalam kajian ini iaitu faktor diri sendiri, latar belakang keluarga, pengaruh rakan sebaya dan permasalahan persekitaran sekolah. Darjah kebolehpercayaan (alfa cronbach) bagi setiap persoalan kajian adalah 0.8536 bagi aspek faktor diri sendiri, 0.7967 bagi aspek latar belakang keluarga, 0.7488 bagi aspek pengaruh rakan sebaya dan 0.6835 bagi aspek permasalahan persekitaran sekolah. Data yang diperolehi dianalisis dengan menggunakan perisian SPSS (Statistical Package for Social Science, Version 11.5) dan dinyatakan dalam bentuk kekerapan serta peratusan. Dapatan kajian menunjukkan faktor diri sendiri paling mempengaruhi responden terlibat dalam masalah sosial berbanding pengaruh rakan sebaya dan pemasalahan persekitaran sekolah. Walau bagaimanapun, analisis mendapati latar keluarga bukan faktor penting responden terlibat dalam masalah sosial. Akhir sekali, beberapa implikasi kajian terhadap faktor penglibatan remaja didalam masalah sosial dicadangkan dan beberapa cadangan untuk kajian lanjutan juga turut dikemukakan. Katakunci: masalah sosial, remaja