Miracle-dz/UniFAR-FTUs huggingface dataset:
‘Reduced malignancy as a mechanism for longevity in mice with adenylyl cyclase type 5 disruption’

Disruption of adenylyl cyclase type 5 (AC5) knockout (KO) is a novel model for longevity. Because malignancy is a major cause of death and reduced lifespan in mice, the goal of this investigation was to examine the role of AC5KO in protecting against cancer. There have been numerous discoveries in genetically engineered mice over the past several decades, but few have been translated to the bedside. One major reason is that it is difficult to alter a gene in patients, but rather a pharmacological approach is more appropriate. The current investigation employs a parallel construction to examine the extent to which inhibiting AC5, either in a genetic knockout (KO) or by a specific pharmacological inhibitor protects against cancer. This study is unique, not only because a combined genetic and pharmacological approach is rare, but also there are no prior studies on the extent to which AC5 affects cancer. We found that AC5KO delayed age‐related tumor incidence significantly, as well as protecting against mammary tumor development in AC5KO × MMTV‐HER‐2 neu mice, and B16F10 melanoma tumor growth, which can explain why AC5KO is a model of longevity. In addition, a Food and Drug Administration approved antiviral agent, adenine 9‐β‐D‐arabinofuranoside (Vidarabine or AraAde), which specifically inhibits AC5, reduces LP07 lung and B16F10 melanoma tumor growth in syngeneic mice. Thus, inhibition of AC5 is a previously unreported mechanism for prevention of cancers associated with aging and that can be targeted by an available pharmacologic inhibitor, with potential consequent extension of lifespan.
Juvenile Fitness for Trial: Lawyer and Youth Justice Officer Professional Survey

Fitness to stand trial is a necessary requisite for a fair trial in judicial proceedings. Research within Australia is limited regarding juvenile fitness for trial, though recent evidence indicates that juvenile offenders are half as likely to be found unfit to stand trial compared to adult offenders. The study surveys lawyers (n = 20) and youth justice workers (n = 20) about their experiences with juveniles in the Queensland youth justice system. Over the preceding 12 months, 133 juveniles were identified as potentially unfit. Intellectual impairment (37%), immaturity (28%), and mental illness (26%) were the most prevalent conditions. Indigenous Australians were rarely referred for mental health evaluation. In comparison, juveniles (mostly non-indigenous) with mental illness and intellectual impairment were significantly more likely to be referred for evaluation. Pragmatic and tactical reasons were most frequently given for non-referral to the Queensland Mental Health Court, which at the time decided fitness.
The Las Campanas/Anglo-Australian Telescope Rich Cluster Survey — III. Spectroscopic studies of X-ray bright galaxy clusters at z~ 0.1

We present the analysis of the spectroscopic and photometric catalogues of 11 X-ray luminous clusters at 0.07 < z < 0.16 from the Las Campanas/Anglo-Australian Telescope Rich Cluster Survey. Our spectroscopic data set consists of over 1600 galaxy cluster members, of which two-thirds are outside r(200). These spectra allow us to assign cluster membership using a detailed mass model and expand on our previous work on the cluster colour-magnitude relation ( CMR) where membership was inferred statistically. We confirm that the modal colours of galaxies on the CMR become progressively bluer with increasing radius d( B - R)/dr(p) = - 0.011 +/- 0.003 and with decreasing local galaxy density d( B - R)/dlog ( Sigma)= - 0.062 +/- 0.009. Interpreted as an age effect, we hypothesize that these trends in galaxy colour should be reflected in mean H delta equivalent width. We confirm that passive galaxies in the cluster increase in Hd line strength as dH delta/dr(p) = 0.35 +/- 0.06. Therefore, those galaxies in the cluster outskirts may have younger luminosity-weighted stellar populations; up to 3 Gyr younger than those in the cluster centre assuming d( B - R)/dt = 0.03 mag per Gyr. A variation of star formation rate, as measured by [ O II]lambda 3727 angstrom, with increasing local density of the environment is discernible and is shown to be in broad agreement with previous studies from the 2dF Galaxy Redshift Survey and the Sloan Digital Sky Survey. We divide our spectra into a variety of types based upon the MORPHs classification scheme. We find that clusters at z similar to 0.1 are less active than their higher-redshift analogues: about 60 per cent of the cluster galaxy population is non-star forming, with a further 20 per cent in the post-starburst class and 20 per cent in the currently active class, demonstrating that evolution is visible within the past 2 - 3 Gyr. We also investigate unusual populations of blue and very red non-star forming galaxies and we suggest that the former are likely to be the progenitors of galaxies which will lie on the CMR, while the colours of the latter possibly reflect dust reddening. We show that the cluster galaxies at large radii consist of both backsplash ones and those that are infalling to the cluster for the first time. We make a comparison to the field population at z similar to 0.1 and examine the broad differences between the two populations. Individually, the clusters show significant variation in their galaxy populations which we suggest reflects their recent infall histories.
Young Adult Propensity to Join Voluntary Associations: The Role of Civic Engagement and Motivations

Based on a self-selection perspective, this study analyzes the relation between personal civic engagement and the willingness to join a voluntary association by considering the mediating role of motivations. Four factors of civic engagement are considered: civic duty, civic skills, social connection, and civic participation; in addition, three motivations are considered: instrumental, expressive, and career-related. The proposed model is tested using a survey of 408 Spanish young adults with no previous experience as members of any association. The analytical technique used is partial least squares. The findings point that instrumental motivation is the main vehicle to translate the four factors of civic engagement into associational involvement, whereas expressive motivation represents a secondary mediator in the effects of civic duty and social connection. Career-related motivation is not associated with the willingness to join a voluntary association. Implications for the recruitment and retention of young adults in voluntary associations are discussed.
On the role of lower bounds in characterizations of weak lower semicontinuity of multiple integrals

Abstract We study weak lower semicontinuity of integral functionals in W 1,p under standard p-growth conditions, with integrands whose negative part may have p-growth as well. A characterization is obtained which, besides quasiconvexity of the integrand, involves a second condition that in general is weaker than boundary quasiconvexity at zero as defined by Ball and Marsden, although for p-homogeneous integrands, it reduces to the latter.
Professional Commitment of Secondary School Teachers in Relation to Their Gender and Area : A Comparative Study

Professional Commitment of teachers has been identified as one of the most critical factors for the future success of education and school. The present study is conducted to evaluate the professional Commitment of secondary school teachers in relation to their gender and area. For present study the sample size taken is 600 teachers of secondary schools of six districts in Uttar Pradesh. Six hundred teachers comprising male and female, rural and urban teachers were selected as a sample of the study. Professional Commitment scale for teachers developed by Dr. Ravinder Kaur, Dr. Sarbjit Kaur Ranu and Mrs. Sarvjeet Kaur Brar was used to collect the data. The finding of the study revealed that professional commitment of secondary school teachers differ significantly in relation to gender and teachers do not significantly differ in professional commitment in relation to area.
The Effect of Binaural Beats on Visuospatial Working Memory and Cortical Connectivity

Binaural beats utilize a phenomenon that occurs within the cortex when two different frequencies are presented separately to each ear. This procedure produces a third phantom binaural beat, whose frequency is equal to the difference of the two presented tones and which can be manipulated for non-invasive brain stimulation. The effects of binaural beats on working memory, the system in control of temporary retention and online organization of thoughts for successful goal directed behavior, have not been well studied. Furthermore, no studies have evaluated the effects of binaural beats on brain connectivity during working memory tasks. In this study, we determined the effects of different acoustic stimulation conditions on participant response accuracy and cortical network topology, as measured by EEG recordings, during a visuospatial working memory task. Three acoustic stimulation control conditions and three binaural beat stimulation conditions were used: None, Pure Tone, Classical Music, 5Hz binaural beats, 10Hz binaural beats, and 15Hz binaural beats. We found that listening to 15Hz binaural beats during a visuospatial working memory task not only increased the response accuracy, but also modified the strengths of the cortical networks during the task. The three auditory control conditions and the 5Hz and 10Hz binaural beats all decreased accuracy. Based on graphical network analyses, the cortical activity during 15Hz binaural beats produced networks characteristic of high information transfer with consistent connection strengths throughout the visuospatial working memory task.
The Effects of Self-presentation on an Expressive Writing Task for Trauma Survivors

Expressive Writing (EW) involves writing in an emotionally expressive manner about an experienced event, and has been shown to be related to increases in psychological and physical well-being. The purpose of the current study was to extend previous work by examining how self-presentation affects psychological and physical gains following the EW task. Forty one participants who have experienced a traumatic event were recruited from the community and given either an EW or control activity. Measures assessing self-presentation and various indices of health were administered at baseline and again at one month follow-up to determine changes in symptomatology. Results indicated that higher levels of perfectionistic self-presentation were significantly associated with less improvement in symptoms of depression and posttraumatic stress. Further, a nonsignificant moderation trend emerged whereby higher levels of perfectionistic self-presentation were associated with more improvement in symptoms of depression and posttraumatic stress in the EW group but not control group.
Isolation and characterization of a 60-70-kD plasma membrane glycoprotein involved in the contact-dependent inhibition of growth

Previous studies have shown that plasma membrane compounds are involved in the contact-dependent inhibition of growth of human diploid fibroblasts. The purification of the active plasma membrane glycoprotein is described in this report. The glycoprotein has an apparent molecular mass of 60-70 kD and, due to differential sialylation, isoelectric points between pH 5.5. and 6.2. Treatment with sialidase yielded one spot in two-dimensional gel electrophoresis with an isoelectric point of 6.3. After removal of the N-glycosidically linked oligosaccharide chains, the apparent molecular mass is reduced by approximately 22 kD. Treatment was diluted NaOH, which removes the O- glycosidically linked portion of oligosaccharides, resulted in a reduction of the apparent molecular mass by approximately 5 kD. The addition of 50 ng/ml of this glycoprotein-for which the term "contactinhibin" is proposed-in immobilized form to sparsely seeded human fibroblasts resulted in a reversible 70-80% inhibition of growth. The inhibition was not confined to human fibroblasts as other cells were also inhibited, with the exclusion of transformed cells, which are refractory to contactinhibin. The inhibitory activity was abolished by treatment with beta-galactosidase or glycopeptidase F, indicating that the glycan moiety is the biologically active part of the molecule. Confluent cultures treated with antibodies raised against contactinhibin were released from the contact-dependent inhibition of growth. In addition to enhanced saturation density, these cultures exhibited a crisscross growth pattern and the formation of foci. Immunocytochemical studies showed that contactinhibin was associated with vimentin. Furthermore, contactinhibin was found to be not expressed in a species- or organ-specific manner.
Brain Dynamics Underlying Training-Induced Improvement in Suppressing Inappropriate Action

Inhibitory control, a core component of executive functions, refers to our ability to suppress intended or ongoing cognitive or motor processes. Mostly based on Go/NoGo paradigms, a considerable amount of literature reports that inhibitory control of responses to “NoGo” stimuli is mediated by top-down mechanisms manifesting ∼200 ms after stimulus onset within frontoparietal networks. However, whether inhibitory functions in humans can be trained and the supporting neurophysiological mechanisms remain unresolved. We addressed these issues by contrasting auditory evoked potentials (AEPs) to left-lateralized “Go” and right NoGo stimuli recorded at the beginning versus the end of 30 min of active auditory spatial Go/NoGo training, as well as during passive listening of the same stimuli before versus after the training session, generating two separate 2 × 2 within-subject designs. Training improved Go/NoGo proficiency. Response times to Go stimuli decreased. During active training, AEPs to NoGo, but not Go, stimuli modulated topographically with training 61–104 ms after stimulus onset, indicative of changes in the underlying brain network. Source estimations revealed that this modulation followed from decreased activity within left parietal cortices, which in turn predicted the extent of behavioral improvement. During passive listening, in contrast, effects were limited to topographic modulations of AEPs in response to Go stimuli over the 31–81 ms interval, mediated by decreased right anterior temporoparietal activity. We discuss our results in terms of the development of an automatic and bottom-up form of inhibitory control with training and a differential effect of Go/NoGo training during active executive control versus passive listening conditions.
Do Demographics Matter?

This study examines individual differences in perceived news media corruption (PNMC), by conducting a face-to-face survey on a representative sample of the Serbian population (N = 544). Extremely high levels of PNMC were found, as well as significant differences in PNMC scores for gender, education level, socioeconomic status, political affiliation, and membership in majority ethnic and religious groups. Corruption perception persona types are created and results are discussed in terms of importance of societal integration for PNMC.
Why Don't You Try Harder? An Investigation of Effort Production in Major Depression

Depression is mainly characterized as an emotional disorder, associated with reduced approach behavior. It remains unclear whether the difficulty in energising behavior relates to abnormal emotional states or to a flattened response to potential rewards, as suggested by several neuroimaging studies. Here, we aimed to demonstrate a specific incentive motivation deficit in major depression, independent of patients' emotional state. We employed a behavioral paradigm designed to measure physical effort in response to both emotional modulation and incentive motivation. Patients did exert more effort following emotionally arousing pictures (whether positive or negative) but not for higher monetary incentives, contrary to healthy controls. These results show that emotional and motivational sources of effort production are dissociable in pathological conditions. In addition, patients' ratings of perceived effort increased for high incentives, whereas controls' ratings were decreased. Thus, depressed patients objectively behave as if they do not want to gain larger rewards, but subjectively feel that they try harder. We suggest that incentive motivation impairment is a core deficit of major depression, which may render everyday tasks abnormally effortful for patients.
Survival of patients with intermediate stage hepatocellular carcinoma treated with superselective transarterial chemoembolization using doxorubicin-loaded DC Bead under cone-beam computed tomography control

Abstract Background The purpose of this retrospective study was to evaluate treatment response, adverse events and survival rates of patients with intermediate stage HCC treated with superselective doxorubicin-loaded DC Bead transarterial chemoembolization (DEBDOX) under cone beam computed tomography (CBCT) control. Patients and methods Between October 2010 and June 2012, 35 consecutive patients with intermediate stage HCC (32 male, 3 female; average age, 67.5 ± 7.8 years; 22 patients Child-Pugh class A, 8 class B, 5 without cirrhosis) were treated with DEBDOX TACE. Portal vein thrombosis was observed in 6 (17.1%) patients. DEBDOX TACE was performed by superselective catheterization of feeding vessels followed by embolization with 100-300 μm microspheres loaded with 50-100 mg of doxorubicin. In all cases, CBCT was used during chemoembolization. Tumor response rates were defined according to mRECIST criteria. Results Overall, 120 procedures were performed (mean, 3.2 per patients). We treated 97 lesions with an average diameter of 4.9 ± 1.9 cm. There were 32 minor and 2 (1.6%) major complications (one liver abscess and one cerebrovascular insult). After a mean follow-up of 27.7 ± 10.5 months, 94.3% of patients achieved an objective response to treatment (42.4% complete response and 57.6% partial response). Mean time to progression was 10.9 ± 5.3 months. Mean overall survival was 33.9 months (95% CI; 28.9 – 38.9 months), with 1- and 2- year survival of 97.1% and 65.7%, respectively. Conclusions Superselective DEBDOX TACE performed under CBCT control is a safe and effective method with high rates of tumor response and overall survival.
CRISPR Is an Optimal Target for the Design of Specific PCR Assays for Salmonella enterica Serotypes Typhi and Paratyphi A

Background Serotype-specific PCR assays targeting Salmonella enterica serotypes Typhi and Paratyphi A, the causal agents of typhoid and paratyphoid fevers, are required to accelerate formal diagnosis and to overcome the lack of typing sera and, in some situations, the need for culture. However, the sensitivity and specificity of such assays must be demonstrated on large collections of strains representative of the targeted serotypes and all other bacterial populations producing similar clinical symptoms. Methodology Using a new family of repeated DNA sequences, CRISPR (clustered regularly interspaced short palindromic repeats), as a serotype-specific target, we developed a conventional multiplex PCR assay for the detection and differentiation of serotypes Typhi and Paratyphi A from cultured isolates. We also developed EvaGreen-based real-time singleplex PCR assays with the same two sets of primers. Principal findings We achieved 100% sensitivity and specificity for each protocol after validation of the assays on 188 serotype Typhi and 74 serotype Paratyphi A strains from diverse genetic groups, geographic origins and time periods and on 70 strains of bacteria frequently encountered in bloodstream infections, including 29 other Salmonella serotypes and 42 strains from 38 other bacterial species. Conclusions The performance and convenience of our serotype-specific PCR assays should facilitate the rapid and accurate identification of these two major serotypes in a large range of clinical and public health laboratories with access to PCR technology. These assays were developed for use with DNA from cultured isolates, but with modifications to the assay, the CRISPR targets could be used in the development of assays for use with clinical and other samples.
Phonocardiogram noise detection in realistic environments

One of the main obstacles that stalls the widespread use of phonocardiograms (PCGs) in modern day medicine is the various noise components they invariably contain. Although many advances have been made towards automated heart sound segmentation and heart pathology detection and classification, an efficient method for noise handling would come as a major aid for further development in this field, especially when it comes to working with PCGs collected in realistic environments such as hospitals and clinics. The use of a digital stethoscope to collect experimental samples of heart sounds in realistic conditions results in highly noise contaminated PCGs. Besides the fact that these environments are often crowded, the very method of data gathering (the use of a transducer for heart sound recording) contributes to the addition of background noise. Thus, only an adaptive algorithm that takes all these aspects into account will successfully increase signal-to-noise ratio. After a process of investigating and testing commonly used PCG noise reduction and noise detection techniques, we have selected a noise detection algorithm based on periodicity measures to classify noisy and clean heart sound segments in PCGs collected in realistic environments. This thesis describes the process of selection, adaptation and testing of this algorithm. We will describe in detail the alterations performed in the previous implementation of this algorithm in order to enable processing of PCGs that contain high noise levels. The results achieved are satisfactory for the current stage of development and the conclusions taken clearly indicate a future line of work towards a robust noise detection algorithm that will aid signal processing of PCGs.
A correlation game for unsupervised learning yields computational interpretations of Hebbian excitation, anti-Hebbian inhibition, and synapse elimination

Much has been learned about plasticity of biological synapses from empirical studies. Hebbian plasticity is driven by correlated activity of presynaptic and postsynaptic neurons. Synapses that converge onto the same neuron often behave as if they compete for a fixed resource; some survive the competition while others are eliminated. To provide computational interpretations of these aspects of synaptic plasticity, we formulate unsupervised learning as a zero-sum game between Hebbian excitation and anti-Hebbian inhibition in a neural network model. The game formalizes the intuition that Hebbian excitation tries to maximize correlations of neurons with their inputs, while anti-Hebbian inhibition tries to decorrelate neurons from each other. We further include a model of synaptic competition, which enables a neuron to eliminate all connections except those from its most strongly correlated inputs. Through empirical studies, we show that this facilitates the learning of sensory features that resemble parts of objects.