Miracle-dz/UniFAR-FTUs huggingface dataset:
Detrimental Effects of Paraquat on Astrocytes-Regulating Synaptic Functions

Paraquat (PQ) exposure is known as a risk factor for developing neurodegenerative diseases. Astrocytes are implicated and affected in neurodegenerative diseases and brain injuries, so it is suspected that PQ may impose detrimental effects on astrocytes function. Here, we present a study of the effects of PQ on synaptic function of astrocytes. Human astrocytes (HA1800) were separately treated with PQ at 200, 400, and 800 µmol/L for 12, 24, and 48 hours, respectively. The concentrations of membrane cholesterol, intracellular glutamate, and adenosine triphosphate (ATP) were assessed by measuring changes with Kits after PQ treatment. Expression levels of glial fibrillary acidic protein (GFAP) and apolipoprotein E (ApoE) were detected using Western blot method. The results indicated that astrocytes were highly susceptible to PQ showing astrogliosis characterized by enhanced GFAP expression (P < .05). The decreased cholesterol level and ApoE expression were observed in PQ-treated astrocytes and so were the decreased levels of glutamates and ATP in PQ-treated astrocytes. These results suggested that PQ exerted the detrimental effects on synaptic function of astrocytes, which may be involved in the progressive process of neurodegenerative disorders.
A study on gingival enlargement and folic acid levels in phenytoin-treated epileptic patients: Testing hypotheses

Background: There have been studies that report clinical benefits of the use of folic acid as an adjuvant to the antiepileptic therapy in the prevention of antiepileptic drug-induced gingival enlargement. However, studies in the past have also reported precipitation of epileptic attacks in patients on folic acid adjuvant therapy due to fall in sera levels of phenytoin due to drug interactions. The study was planned to investigate the association of phenytoin-induced gingival enlargement and sera levels of folic acid in epileptic patients on phenytoin therapy. The statistical analysis was done using t-test and the baseline serum folate levels and the serum folate levels obtained after 6 months of phenytoin therapy were correlated with the respective grades of gingival enlargement using Pearson's coefficient formula. Methods: A total of 25 patients aged between 18 and 50 years, clinically diagnosed with epilepsy prior to the start of phenytoin therapy were included based on selection criteria and written informed consents were obtained. Assessment of serum folic acid levels and gingival enlargement was done prior to the start of and after 6 months of phenytoin therapy. Results: The results of the study confirmed a significant association between low serum folate levels with increasing severity as well as an early onset of phenytoin-induced gingival enlargement. Conclusions: The results of the study suggest a higher incidence of gingival enlargement in phenytoin treated epileptic patients with a positive correlation with falling serum folic acid levels as the duration of the therapy increases.
Exploring Physical and Chemical Factors Influencing the Properties of Recombinant Prion Protein and the Real-Time Quaking-Induced Conversion (RT-QuIC) Assay

Real-time quaking-induced conversion (RT-QuIC), a highly specific and sensitive assay able to detect low levels of the disease-inducing isoform of the prion protein (PrPd) in brain tissue biopsies and cerebral spinal fluid, has great potential to become a method for diagnosing prion disease ante mortem. In order to standardize the assay method for routine analysis, an understanding of how physical and chemical factors affect the stability of the recombinant prion protein (rPrP) substrate and the RT-QuIC assay’s sensitivity, specificity, and reproducibility is required. In this study, using sporadic Creutzfeldt-Jakob Disease brain homogenate to seed the reactions and an in vitro-expressed recombinant prion protein, hamster rPrP, as the substrate, the following factors affecting the RT-QuIC assay were examined: salt and substrate concentrations, substrate storage, and pH. Results demonstrated that both the generation of the quality and quantities of rPrP substrate critical to the reaction, as well as the RT-QuIC reaction itself required strict adherence to specific physical and chemical conditions. Once optimized, the RT-QuIC assay was confirmed to be a very specific and sensitive assay method for sCJD detection. Findings in this study indicate that further optimization and standardization of RT-QuIC assay is required before it can be adopted as a routine diagnostic test.
Hoarding and Minimalism: Tendencies in Digital Data Preservation

Digital data, from texts to files and mobile applications, has become a pervasive component of our society. With seemingly unlimited storage in the cloud at their disposal, how do people approach data preservation, deciding what to keep and discard? We interviewed 23 participants with diverse backgrounds, asking them about their perceived digital data: what "stuff" they kept through the years, why, how they used it, and what they considered important. In an iterative analysis process, we uncovered a spectrum of tendencies that drive preservation strategies, with two extremes: hoarding (where participants accumulated large amounts of data, even if considered of little value) and minimalism (where they kept as little as possible, regularly cleaning their data). We contrast and compare the two extremes of the spectrum, characterize their nuanced nature, and discuss how our categorization compares to previously reported behaviors such as filing and piling, email cleaners and keepers. We conclude with broad implications for shaping technology.
From Tangible to Augmented: Designing a PhonoBlocks Reading System Using Everyday Technologies

This paper presents an augmented reality (AR) system that supports early reading and spelling acquisition of English for children. The design of the AR system was based on and extends our prior tangible reading system called PhonoBlocks. In this paper, we discuss why and how we extend the work from PhonoBlocks to an AR system design. The goal of our system is to teach children explicit letter-sound correspondences. Two key design features of our system, which are different from other systems, are the use of augmented dynamic colour cues and 3D physical lowercase letters that help to draw children's attention to how letters' positions in words change letter sounds. Unlike our previous system, our AR system uses off-the-shelf technology so it can be easily scaled and distributed. We discuss the advantages and opportunities for our AR solution, and outline our future plans for evaluating this system.
“An Odd Kind of Pleasure”: Differentiating Emotional Challenge in Digital Games

Recent work introduced the notion of emotional challenge as a means to afford more unique and diverse gaming experiences. However, players' experience of emotional challenge has received little empirical attention. It remains unclear whether players enjoy it and what exactly constitutes the challenge thereof. We surveyed 171 players about a challenging or an emotionally challenging experience, and analyzed their responses with regards to what made the experience challenging, their emotional response, and the relation to core player experience constructs. We found that emotional challenge manifested itself in different ways, by confronting players with difficult themes or decisions, as well as having them deal with intense emotions. In contrast to more'conventional' challenge, emotional challenge evoked a wider range of negative emotions and was appreciated significantly more by players. Our findings showcase the appeal of uncomfortable gaming experiences, and extend current conceptualizations of challenge in games.
Potential application of chitosan-clay coating on some quality properties of agricultural product during storage

Lemon is cultivated in many countries including of Iran as one of the most important citrus fruit . Because of the split skin and very juicy properties, the quality of lemon fruits has been affected by some environmental factors during storage. In the present study, t he efficiency of chitosan-clay nano-composite coating on some quality properties of lemon was investigated during cold storage . Nano-structural feature of prepared chitosan-clay was approved by X-ray diffraction (XRD) . The chemical and mechanical properties of coated and uncoated fruit samples including of Total Soluble Solid (TSS), Titratable Acid (TA ), and firmness, shear and punch forces were measured after 0 to 63 days cold storage. Coated samples showed significantly the lowest value of TSS and punch force and the highest value TA, and firmness and peel shear forces. Furthermore , coated samples indicated the lowest weight loss during storage . Based on the results of present study, the quality properties of lemon fruit samples was improved after coating with chitosan-clay nano-composite during storage.
The effects of psychoeducational family intervention on coping strategies of relatives of patients with bipolar I disorder: results from a controlled, real-world, multicentric study

Background Psychoeducational family intervention (PFI) has been proven to be effective in improving the levels of family burden and patients’ personal functioning in schizophrenia and bipolar disorders (BDs). Less is known about the impact of PFI on relatives’ coping strategies in BD. Methods A multicenter, controlled, outpatient trial funded by the Italian Ministry of Health and coordinated by the Department of Psychiatry of the University of Campania “Luigi Vanvitelli” has been conducted in patients with bipolar I disorder (BD-I) and their key relatives consecutively recruited in 11 randomly selected Italian community mental health centers. We aim to test the hypothesis that PFI improves problem-oriented coping strategies in relatives of BD-I patients compared to the Treatment As Usual (TAU) group. Results The final sample was constituted of 123 patients and 139 relatives. At baseline assessment (T0), the vast majority of relatives already adopted problem-oriented coping strategies more frequently than the emotion-focused ones. At the end of the intervention, relatives receiving PFI reported a higher endorsement of adaptive coping strategies, such as “maintenance of social interests” (odds ratio [OR]=0.309, CI=0.04–0.57; p=0.023), “positive communication with the patient” (OR=0.295, CI=0.13–0.46; p=0.001), and “searching for information” (OR=0.443, CI=0.12–0.76; p=0.007), compared to TAU relatives, after controlling for several confounders. As regards the emotion-focused coping strategies, relatives receiving the experimental intervention less frequently reported to adopt “resignation” (OR=−0.380, CI=−0.68 to −0.08; p=0.014) and “coercion” (OR=−0.268, CI=−0.46 to −0.08; p=0.006) strategies, compared to TAU relatives. Conclusion PFI is effective in improving the adaptive coping strategies of relatives of BD-I patients, but further studies are needed for evaluating the long-term benefits of this intervention.
Spatial heterogeneities shape the collective behavior of signaling amoeboid cells

Imaging and mathematical analyses reveal how Dictyostelium discoideum cells navigate around obstacles in their environment. Slime mold obstacle course The slime mold Dictyostelium discoideum, which lives in the soil, is an experimental model of cell migration. Starving Dictyostelium cells secrete the chemoattractant cAMP, which stimulates the cells to migrate in head-to-tail streams, eventually leading to the formation of fruiting bodies required for cell survival. Eckstein et al. generated substrates containing pillars of different sizes and patterns and performed mathematical analysis of images of cAMP and the migrating cells to determine how the Dictyostelium cells interpreted waves of chemoattractant disrupted by obstacles. This analysis could be applied to understanding how collections of cells interact with spatial barriers in their environment. In its natural habitat in the forest soil, the cellular slime mold Dictyostelium discoideum is exposed to obstacles. Starving Dictyostelium cells secrete cAMP, which is the key extracellular signaling molecule that promotes the aggregation process required for their long-term survival. Here, we investigated the influence of environmental inhomogeneities on the signaling and pattern formation of Dictyostelium cells. We present experimental data and numerical simulations on the pattern formation of signaling Dictyostelium cells in the presence of periodic arrays of millimeter-sized pillars. We observed concentric cAMP waves that initiated almost synchronously at the pillars and propagated outward. In response to these circular waves, the Dictyostelium cells streamed toward the pillars, forming aggregates arranged in patterns that reflected the periodicity of the lattice of pillars. Our results suggest that, in nature, the excitability threshold and synchronization level of the cells are two key parameters that control the nature of the interaction between cells and spatial heterogeneities in their environment.
3D Structures of Plant Phytochrome A as Pr and Pfr From Solid-State NMR: Implications for Molecular Function

We present structural information for oat phyA3 in the far-red-light-absorbing (Pfr) signaling state, to our knowledge the first three-dimensional (3D) information for a plant phytochrome as Pfr. Solid-state magic-angle spinning (MAS) NMR was used to detect interatomic contacts in the complete photosensory module [residues 1–595, including the NTE (N-terminal extension), PAS (Per/Arnt/Sim), GAF (cGMP phosphodiesterase/adenylyl cyclase/FhlA) and PHY (phytochrome-specific) domains but with the C-terminal PAS repeat and transmitter-like module deleted] auto-assembled in vitro with 13C- and 15N-labeled phycocyanobilin (PCB) chromophore. Thereafter, quantum mechanics/molecular mechanics (QM/MM) enabled us to refine 3D structural models constrained by the NMR data. We provide definitive atomic assignments for all carbon and nitrogen atoms of the chromophore, showing the Pfr chromophore geometry to be periplanar ZZEssa with the D-ring in a β-facial disposition incompatible with many earlier notions regarding photoconversion yet supporting circular dichroism (CD) data. The Y268 side chain is shifted radically relative to published Pfr crystal structures in order to accommodate the β-facial ring D. Our findings support a photoconversion sequence beginning with Pr photoactivation via an anticlockwise D-ring Za→Ea photoflip followed by significant shifts at the coupling of ring A to the protein, a B-ring propionate partner swap from R317 to R287, changes in the C-ring propionate hydrogen-bonding network, breakage of the D272–R552 salt bridge accompanied by sheet-to-helix refolding of the tongue region stabilized by Y326–D272–S554 hydrogen bonding, and binding of the NTE to the hydrophobic side of ring A. We discuss phyA photoconversion, including the possible roles of mesoscopic phase transitions and protonation dynamics in the chromophore pocket. We also discuss possible associations between structural changes and translocation and signaling processes within the cell.
Molecular Characterization of the RNA-Binding Protein Quaking-a in Megalobrama amblycephala: Response to High-Carbohydrate Feeding and Glucose/Insulin/Glucagon Treatment

The RNA-binding protein quaking-a (Qkia) was cloned from the liver of blunt snout bream Megalobrama amblycephala through the rapid amplification of cDNA ends method, with its potential role in glucose metabolism investigated. The full-length cDNA of qkia covered 1,718 bp, with an open reading frame of 1,572 bp, which encodes 383 AA. Sequence alignment and phylogenetic analysis revealed a high degree of conservation (97–99%) among most fish and other higher vertebrates. The mRNA of qkia was detected in all examined organs/tissues. Then, the plasma glucose levels and tissue qkia expressions were determined in fish intraperitoneally injected with glucose [1.67 g per kg body weight (BW)], insulin (0.052 mg/kg BW), and glucagon (0.075 mg/kg BW) respectively, as well as in fish fed two dietary carbohydrate levels (31 and 41%) for 12 weeks. Glucose administration induced a remarkable increase of plasma glucose with the highest value being recorded at 1 h. Thereafter, it reduced to the basal value. After glucose administration, qkia expressions significantly decreased with the lowest value being recorded at 1 h in liver and muscle and 8 h in brain, respectively. Then they gradually returned to the basal value. The insulin injection induced a significant decrease of plasma glucose with the lowest value being recorded at 1 h, whereas the opposite was true after glucagon load (the highest value was gained at 4 h). Subsequently, glucose levels gradually returned to the basal value. After insulin administration, the qkia expressions significantly decreased with the lowest value being attained at 2 h in brain and muscle and 1 h in liver, respectively. However, glucagon significantly stimulated the expressions of qkia in tissues with the highest value being gained at 6 h. Moreover, high dietary carbohydrate levels remarkably increased plasma glucose levels, but down-regulated the transcriptions of qkia in tissues. These results indicated that the gene of blunt snout bream shared a high similarity with that of the other vertebrates. Glucose and insulin administration, as well as high-carbohydrate feeding, remarkably down-regulated its transcriptions in brain, muscle and liver, whereas the opposite was true after the glucagon load.
Phase Retrieval With Random Gaussian Sensing Vectors by Alternating Projections

We consider a phase retrieval problem, where we want to reconstruct a <inline-formula> <tex-math notation="LaTeX">$n$ </tex-math></inline-formula>-dimensional vector from its phaseless scalar products with <inline-formula> <tex-math notation="LaTeX">$m$ </tex-math></inline-formula> sensing vectors, independently sampled from complex normal distributions. We show that, with a suitable initialization procedure, the classical algorithm of alternating projections (Gerchberg–Saxton) succeeds with high probability when <inline-formula> <tex-math notation="LaTeX">$m\geq Cn$ </tex-math></inline-formula>, for some <inline-formula> <tex-math notation="LaTeX">$C>0$ </tex-math></inline-formula>. We conjecture that this result is still true when no special initialization procedure is used, and present numerical experiments that support this conjecture.
Competition Strategy and Geographical Proximity of Contract Research Organizations

We investigate the evidence for geographical proximity of contract research organizations (CROs) in the United States. We examine whether CROs locate near each other for knowledge spillover motivation, or whether they avoid each other as a competition strategy. If they are not located near to each other, then they are not likely to cooperate, or source knowledge from each other. We use a spatial pattern approach and find that CROs are located spatially in the United States. CROs do not consistently access localized knowledge from other CROs, rather they avoid each other and establish in isolated locations to capture the entire market.
Stomatal patchiness and task-performing networks.

BACKGROUND Patchy stomatal conductance is a poorly understood and little-studied phenomenon. It is relatively common, yet it appears to be detrimental to water-use efficiency under some conditions and has no immediately obvious physiological function of any kind. Much of the difficulty in studying patchy stomatal conductance is tied to its unpredictability, both in occurrence and in characteristics. SCOPE AND CONCLUSIONS Statistical analyses of the variability of stomatal patchiness reveal remarkable similarities to structures and behaviours found in locally connected networks of dynamic units that perform tasks. Such systems solve problems that reside at the level of the entire network despite the absence of a central processor or a mechanism for directly sharing information over the entire system. Frequently, task performance is emergent, in the sense that no unit independently performs the task. Because each unit in the network can communicate with only its immediate neighbours, problem solving is accomplished by the states of the individual units self-organizing into synchronized, collective patterns. In some cases, patches of states form and move coherently over the network, thus providing a means for distantly separated parts of the network to communicate. Often, exactly what form these patches take and how they move as the units synchronize is highly unpredictable. In analogy with such networks, it is suggested that stomatal patchiness may be a signature that plants optimize gas exchange in a more sophisticated and adaptive manner than if performed by their individual stomata independently.
“I Hear You”: Understanding Awareness Information Exchange in an Audio-only Workspace

Graphical displays are a typical means for conveying awareness information in groupware systems to help users track joint activities, but are not ideal when vision is constrained. Understanding how people maintain awareness through non-visual means is crucial for designing effective alternatives for supporting awareness in such situations. We present a lab study simulating an extreme scenario where 32 pairs of participants use an audio-only tool to edit shared audio menus. Our aim is to characterise collaboration in this audio-only space in order to identify whether and how, by itself, audio can mediate collaboration. Our findings show that the means for audio delivery and choice of working styles in this space influence types and patterns of awareness information exchange. We thus highlight the need to accommodate different working styles when designing audio support for awareness, and extend previous research by identifying types of awareness information to convey in response to group work dynamics.
Pointing All Around You: Selection Performance of Mouse and Ray-Cast Pointing in Full-Coverage Displays

As display environments become larger and more diverse - now often encompassing multiple walls and room surfaces - it is becoming more common that users must find and manipulate digital artifacts not directly in front of them. There is little understanding, however, about what techniques and devices are best for carrying out basic operations above, behind, or to the side of the user. We conducted an empirical study comparing two main techniques that are suitable for full-coverage display environments: mouse-based pointing, and ray-cast 'laser' pointing. Participants completed search and pointing tasks on the walls and ceiling, and we measured completion time, path lengths and perceived effort. Our study showed a strong interaction between performance and target location: when the target position was not known a priori the mouse was fastest for targets on the front wall, but ray-casting was faster for targets behind the user. Our findings provide new empirical evidence that can help designers choose pointing techniques for full-coverage spaces.