Miracle-dz/UniFAR-FTUs huggingface dataset:
Protease Production Capabilities of Micrococcus Luteus and Bacillus Species Isolated from Abattoir Environment

The study investigated the optimu m conditions of temperature, pH, inoculu m size and time of incubation on bacterial p rotease production. Protease producing bacterial species were isolated fro m abattoir soil and identified as Micrococcus luteus and Bacillus species. The optimu m conditions observed for protease production was 37℃ at pH 7, with 1% inoculu m in the mediu m for 24 h of incubation in Micrococcus luteus while in Bacillus species, the optimu m conditions observed was 47℃ at p H 9, with 2% inoculu m concentration in the med iu m for 96 h of incubation. Generally, temperature and pH had more effect on the protease activity of Micrococcus luteus while inoculu m concentration and time of incubation had more effect on the protease activity of Bacillus species. The study gave evidence that these bacterial isolates could be potentially applied in biotechnological processes.
Assessment Literacy for the Language Classroom

Language testing has seen unprecedented expansion during the first part of the 21st century. As a result there is an increasing need for the language testing profession to consider more precisely what it means by “assessment literacy” and to articulate its role in the creation of new pedagogic materials and programs in language testing and assessment to meet the changing needs of teachers and other stakeholders for a new age. This article describes a research project in which a survey instrument was developed, piloted, and delivered on the Internet to elicit the assessment training needs of language teachers. The results were used to inform the design of new teaching materials and the further development of online resources that could be used to support program delivery. The project makes two significant contributions. First, it provides new empirically derived content for the concept of assessment literacy within which to frame materials development and teaching. Second, it uncovered methodological problems with existing survey techniques that may have impacted upon earlier studies, and solutions to these problems are suggested.
Ser968Thr Mutation within the A3 Domain of Von Willebrand Factor (VWF) in Two Related Patients Leads to a Defective Binding of VWF to Collagen

Summary We report the identification of a new mutation of von Willebrand Factor (VWF) gene within exon 30 occurring in two related patients (mother and daughter) with a hemorrhagic syndrome. A T A transvertion at nucleotide 5441 was found changing the serine 968 to threonine of the mature VWF subunit (S1731T of the preproVWF). The Ser968Thr mutation is located within the VWF A3 domain which interacts with type I and III collagens. Both patients were found to be heterozygous for the mutation. The propositus (daughter) exhibited a slightly prolonged bleeding time, levels of VWF:Ag and VWF:RCo at the lower limit of normal, contrasting with normal levels of VIII:C. Her mother exhibited borderline bleeding time and moderately decreased levels of VWF and VIII:C. In both patients multimeric structure of VWF and ristocetin- as well as botrocetin-induced binding of VWF to GPIb were normal; however both patients repeatedly showed decreased binding of VWF to collagen. The Ser968Thr substitution was reproduced by site-directed mutagenesis on the full-length cDNA of VWF. The mutated recombinant VWF (rVWF), T968rVWF, and the hybrid S/T968rVWF were transiently expressed by COS-7 cells. Both rVWF exhibited normal multimeric pattern and normal ristocetin- as well as botrocetin-induced binding to GPIb. T968rVWF showed significantly decreased binding to collagen while the hybrid S/T968rVWF bound to collagen in a similar way to that of the patients’ plasma VWF. Thus, our data demonstrate that the Ser968Thr mutation of the VWF A3 domain is clearly responsible for the abnormal binding of VWF to collagen observed in both patients. The Ser968Thr substitution of the VWF is the first mutation identified in two patients leading to a decreased affinity of VWF for collagen and a normal multimeric structure.
Regulating the Skin Permeation Rate of Escitalopram by Ion-pair Formation with Organic Acids

In order to regulate the skin permeation rate (flux) of escitalopram (ESP), ion-pair strategy was used in our work. Five organic acids with different physicochemical properties, benzoic acid (BA), ibuprofen (IB), salicylic acid (SA), benzenesulfonic acid (BSA), and p-aminobenzoic acid (PABA), were employed as counter-ions to regulate the permeation rate of ESP across the rabbit abdominal skin in vitro. The interaction between ESP and organic acids was characterized by FTIR and 13C NMR spectroscopy. Results showed that all organic acids investigated in this study performed a controlling effect on ESP flux. To further analyze the factors concerned with the permeation capability of ESP-acid complex, a multiple linear regression model was used. It is concluded that the steady-state flux (J) of ESP-acid complexes had a positive correlation with log Ko/w (the n-octanol/water partition coefficient of ion-pair complex) and pKa (the acidity of organic acid counter-ion), but a negative correlation with MW (the molecular weight of ion-pair complex). The logKo/w of ion-pair complex is the primary one in all the factors that influence the skin permeation rate of ESP. The results demonstrated that organic acid with appropriate physicochemical properties can be considered as suitable candidate for the transdermal drug delivery of escitalopram.
Maternal Deprivation Influences Pup Ultrasonic Vocalizations of C57BL/6J Mice

Maternal deprivation (MD) is frequently used as an early life stress model in rodents to investigate behavioral and neurological responses under stressful conditions. However, the effect of MD on the early postnatal development of rodents, which is when multiple neural systems become established, is rarely investigated due to methodological limitations. Ultrasonic vocalizations (USVs) are one of the few responses produced by neonatal rodents that can be quantitatively analyzed, and the quantification of USVs is regarded as a novel approach to investigate possible alterations in the neurobehavioral and emotional development of infant rodents under stress. To investigate the effect of MD on pup mice, we subjected C57BL/6J mice to MD and recorded the USVs of pups on postnatal days 1, 3, 7, 8, and 14. To determine whether the effect of MD on USVs was acute or cumulative, pre- and post-separation USV groups were included; sex differences in pup USV emission were also investigated. Our results suggest that (i) USV activity was high on postnatal days 3–8; (ii) the MD effect on USVs was acute, and a cumulative effect was not found; (iii) the MD mice vocalized more and longer than the controls at a lower frequency, and the effect was closely related to age; and (iv) female pups were more susceptible than males to the effect of MD on USV number and duration between postnatal days 3–8.
Production and Characterization of a Nitrilase from Pseudomonas aeruginosa RZ44 and its Potential for Nitrile Biotransformation

Background The conversion of nitriles into amides or carboxylic acids by nitrilase has taken its application into consideration, as the scope of its applications has recently been extended. Objectives In this study, P. aeruginosa RZ44 was isolated from sewage in the Kerman which has Nitrile-degradation activity. In order to improve the nitrilase production, several optimization were done on environmental condition. Nitrilase activity was characterized against different pHs, temperatures, ions, and substrates. Materials and Methods Enzyme activity was evaluated by determining the production of ammonia following to the modification of the phenol/hypochlorite method. Different factors that affect production of the enzyme by P. aeruginosa RZ44 were optimized and evaluated in the culture mediums. Results The results showed that degradation of the acetonitrile by P. aeruginosa RZ44 increased the pH of the growth medium from the initial pH 7.0 to 9.37. Optimizing the medium for P. aeruginosa RZ44, it was found that glucose and starch (5 g.L-1) have strongly supported nitrilase production, compared to the control. As well, urea (5 g.L-1) and yeast extract (15 g.L-1) have favored an increased biomass and nitrilase production, as the nitrogen sources. These results show that nitrilase production increases in the pH range 5.0 to 7.0 and then start decreasing. Addition of the Mg2+, Fe2+ and Na+ has supported the biomass and nitrilase production. Co2+, Mn2+ and Cu2+ were confirmed to inhibit cell growth and enzyme production. Enzyme characterization results show that, P. aeruginosa RZ44 nitrilase exhibits comparatively high activity and stability at pH 7.0 and 40°C. Nitrilase was completely inhibited by CoCl2 and CaCl2, whereas, the inhibition in the presence of MnSO4 and CuSO4 was about 60%. Time course analysis of the nitrile conversion by the resting P. aeruginosa RZ44 cells showed that nitrile substrates (i.e. acetonitrile) was hydrolyzed within 8 h. Conclusions these results indicate that P. aeruginosa RZ44 has the potential to be applied in the biotransformation of nitrile compounds.
Width of the latitude of acceptance as a determinant of attitude change.

In a study examining the implications of social judgment theory for predicting individual differences in attitude change, a subject received a message advocating a position on birth control either mildly, moderately, or strongly discrepant from his own. For all three levels of discrepancy, the subjects with wide latitudes of acceptance on the issue showed greater attitude change than did subjects with either narrow or medium latitudes of acceptance. Since a mildly discrepant message was in the lattitude of acceptance even for narrow latitude subjects and a strongly discrepant message in the latitude of rejection even for wide latitude subjects, this finding cannot be reconciled with social judgment theory. Since correlations between latitude widths on birth control and two irrelevant issues were low and latitude width on the irrelevant issues did not relate to attitude change on birth control, width of the latitude of acceptance was interpreted as an issue-specific index of influenceabil ity. Category width, though independent of latitude of acceptance width, also predicted attitude change: narrow categorizers changed their attitudes more than did either medium or broad categorizers. Central to the social judgment approach to attitude change (Sherif, Sherif, & Nebergall, 1965; Sherif & Hovland, 1961) is the assumption that the structure of an individual's attitude is a determinant of receptivity to a persuasive communication. Social judgment theory specifies attitude structure by dividing the attitude dimension into "latitudes" or regions: a latitude of acceptance, which is the range of positions that an individual accepts, and a latitude of rejection, which is the range of positions that he rejects.4 According to the major hypothesis of
Maxmin Expected Utility over Savage Acts with a Set of Priors

When do dynamic nonconvexities at the disaggregate level translate into dynamic nonconvexities at the aggregate level? We address this question in a framework where the production of differentiated intermediate inputs is subject to dynamic nonconvexities and show that the answer depends on the degree of Hicks-Allen complementarity (substitutability) between differentiated inputs. In our simplest model, a generalization of Judd (1985) and Grossman and Helpman (1991) among many others, there are dynamic nonconvexities at the aggregate level if and only differentiated inputs are Hicks-Allen complements. We also compare dynamic equilibrium and optimal allocations in the presence of aggregate dynamic nonconvexities due to Hicks-Allen complementarities between differentiated inputs.
Conceptualizing Women's Fear of Sexual Assault on Campus

Sexual assault is increasingly recognized as an urgent and pervasive problem on university campuses. Women's fear of sexual assault is similarly significant and widespread. Growing university assault prevention efforts on campus have often over-looked the negative implications of women's fear. Depending on their onus of responsibility, university sexual assault prevention strategies may implicitly reinforce gendered social norms for public behavior by increasing women's fear in public spaces, with consequent detrimental effects for women's positive experience on campus. Based on a conceptualization of fear of sexual assault as a form of social control, this review discusses societal, individual, and university/campus factors (organizational, social, and especially physical) associated with women's fear of assault on campus. In conclusion, it suggests university assault prevention strategies that may also reduce women's fear.
Outcomes of simultaneous laparoscopic colorectal and hepatic resection for patients with colorectal cancers: a comparative study.

BACKGROUND Although laparoscopic colorectal resection and laparoscopic liver resection have been accepted as effective alternatives to conventional open procedures, there are only a few reports on the clinical availability of simultaneous performance of these two procedures. We report our collective experience of patients with colorectal cancers treated with totally laparoscopic colorectal and liver resection, in comparison with those treated with an open approach. PATIENTS AND METHODS This study is a retrospective, case-match review of prospectively collected data. Between May 2008 and December 2012, 24 patients with primary colorectal cancer and associated hepatic lesions underwent simultaneous laparoscopic colorectal and liver resection (laparoscopic group). They were matched with patients who underwent an open procedure (open group; n=24 out of 232) based on the types of surgery. Patient demographics, operative details, tumor-related parameters, and postoperative outcomes were analyzed. RESULTS Demographic features and pathologic outcomes were similar in both groups. The median duration of operation was significantly longer in the laparoscopic group than in the open group (290 versus 244 minutes; P=.008), and the median estimated blood loss was larger (325 versus 250 mL; difference not significant, P=.35). However, the time to starting a soft blended diet (3.0 versus 4.5 days; P<.001) and postoperative stay (8.0 versus 10.5 days; P=.001) in the laparoscopic group were both significantly shorter than in the open group. The postoperative complication rate was lower in the laparoscopic group (17% versus 42%; difference not significant, P=.06). The minor complication rate was significantly lower in the laparoscopic group (4% versus 33%; P=.02). CONCLUSIONS A totally laparoscopic approach might provide short-term benefits associated with enhanced postoperative recovery despite a longer procedure time and larger blood loss. It can be a reasonable option for simultaneous colorectal and hepatic resection.
Design and experiments of advanced leg module (HRP-2L) for humanoid robot (HRP-2) development

This paper presents an advanced leg module developed for HRP-2, a new humanoid robotics platform, which has been developed in phase two of the Humanoid Robotics Project (HRP), a five year program sponsored by the Ministry of Economy, Trade and Industry of Japan (METI) from 1998FY to 2002FY. The biped locomotion ability of HRP-1, the humanoid robotics platform developed in phase I is to be improved so that HRP-2 can cope with rough terrain and can prevent possible damage to the robot body caused by tipping over. In this paper, the mechanisms and specifications of the leg module, the electrical system, the simulation results utilized for deciding specifications, and some experimental results are presented.
What's being used at home: a household pesticide survey.

OBJECTIVE Since very little is known about the health effects that household pesticides have on children, we conducted this survey to identify what pesticides are being used in the home, where they are being used and stored, and what methods are used for their disposal. METHODS In the spring of 1999 we conducted a survey in a community in the state of Arizona, in the United States of America, on the border with Mexico. To be eligible to participate in the survey, households had to have used a pesticide in the 6 mo prior to the survey and to have at least one child under the age of 10 years. We gathered general information on pesticide usage, storage, and disposal, in addition to specific information about each of the pesticides currently being used and/or stored in the home. RESULTS In the 107 households surveyed, we found 148 pesticide products, for a mean of 1.4 per household. Half of the pesticides were stored less than 4 feet (1.22 m) from the ground, at a level a child could reach. Seventy percent of all the pesticides were stored inside the home, with the kitchen being the storage room most often mentioned. The kitchen was also the room where most of the pesticides were used, with 69% of the respondents saying they had used at least one pesticide there. CONCLUSIONS From our research we conclude that it will be important to continue to investigate all avenues of pesticide exposure in order to fully evaluate childhood exposures. Understanding household pesticide use and developing a model of exposure will help in this process. Profiles of the use, storage, and disposal of products will also guide the development of effective education and poison prevention programs in the community.
Robust Estimator for Non-Line-of-Sight Error Mitigation in Indoor Localization

Indoor localization systems are undoubtedly of interest in many application fields. Like outdoor systems, they suffer from non-line-of-sight (NLOS) errors which hinder their robustness and accuracy. Though many ad hoc techniques have been developed to deal with this problem, unfortunately most of them are not applicable indoors due to the high variability of the environment (movement of furniture and of people, etc.). In this paper, we describe the use of robust regression techniques to detect and reject NLOS measures in a location estimation using multilateration. We show how the least-median-of-squares technique can be used to overcome the effects of NLOS errors, even in environments with little infrastructure, and validate its suitability by comparing it to other methods described in the bibliography. We obtained remarkable results when using it in a real indoor positioning system that works with Bluetooth and ultrasound (BLUPS), even when nearly half the measures suffered from NLOS or other coarse errors.
Do Victims of Injustice Punish to Improve Their Mood?

Previous research has shown that angry people sometimes aggress to improve their mood. The present research tests whether people who have been treated unjustly seek revenge (i.e., retributive punishment) to improve their mood. The authors hypothesized that retributive punishment is generally not fuelled by a desire to improve one’s mood, whereas more destructive forms of aggression (e.g., venting one’s anger against people or inanimate objects) are. In Study 1, half of the participants were led to believe that their mood was frozen. In Study 2, half of the participants were led to believe that their mood would automatically improve at the end of the study by visiting a “good mood room” with snacks, beverages, and media. Retributive punishment was not affected by either mood management manipulation, whereas destructiveness was. Study 3 expands these findings by showing that retributive reactions were only reduced by a mood improvement manipulation when self-focus was induced.
Low power counting via collaborative wireless communications

Metrics that aggregate the state of neighboring nodes are frequently used in wireless sensor networks. In this paper, we present two primitives that exploit simultaneous communications in 802.15. 4 radios to enable a polling node to calculate with low power the number (or set) of its neighbors where some state predicate of interest holds. In both primitives, the poller assigns transmission powers and response lengths to its respective neighbors for their simultaneous response to each of its poll requests. The two primitives adopt complementary schemes for power assignment such that the Received Signal Strength Indicator (RSSI) of the respective signal from each neighbor is significantly different from that of all others in one primitive and nearly equivalent to that of the others in the other. The first primitive, LinearPoll, suits sparse networks and consumes energy that is linear in the size of its neighborhood, whereas the second primitive, LogPoll, suits dense networks and consumes constant energy. Compared to the state-of-the-art solutions that use multiple sub-carriers, our primitives are simpler and more compute-efficient while provide estimation with comparable quality. Compared to single-carrier solutions, our primitives achieve comparable quality at less than half the energy cost or richer information at comparable energy cost. They are also compatible with other radio physical layers. Based on our implementation for CC2420 radios on the TelosB platform, we evaluate the primitives in different wireless environments and neighborhood topologies to study their performance, the tradeoff between their estimation accuracy and energy cost, and methods for tuning their critical parameters, and we compare them with baseline counting protocols.
Unified Cooperative Control of Multiple Agents on a Sphere for Different Spherical Patterns

In this technical note, we consider the cooperative control of multiple agents on a sphere, provide the appropriate attraction-repulsion interactions between agents with introduction of the state-dependent repulsive coefficients, and design the unified control laws for agents to achieve three fundamental spherical configurations or patterns: rendezvous, uniform deployment, and formation, using both the first- and second-order models. We present the analysis of the stability, scaling and geometric patterns of the models on the sphere under different attraction-repulsion parameters, with illustration of the geometrical patterns and the geometrical isomerism by simulations. The closed-loop dynamics of agents are invariant with respect to group rotation actions on the sphere.
Sources of differential participation rates in school science: the impact of curriculum reform

School science courses have widely varying participation rates across a range of student characteristics. One of the stated aims of the 2006 Key Stage 4 science curriculum reforms in England was to improve social mobility and inclusion. To encourage students to study more science, this reform was followed by the introduction in 2008 of an entitlement to study the three separate sciences at Key Stage 4 for the more highly attaining students. This paper uses longitudinal national data over a five year period to investigate the extent and change of participation across science courses at KS4, focussing on student gender and socio-economic status. It finds that whilst there is some evidence of a move towards a more equitable gender balance for some courses, there is as yet little evidence of substantial change in differential participation rates by socio-economic status.