Miracle-dz/UniFAR-FTUs huggingface dataset:
Talin1 Regulates Integrin Turnover To Promote Embryonic Epithelial Morphogenesis

ABSTRACT Talin is a cytoskeletal protein that binds to integrin β cytoplasmic tails and regulates integrin activation. Talin1 ablation in mice disrupts gastrulation and causes embryonic lethality. However, the role of talin in mammalian epithelial morphogenesis is poorly understood. Here we demonstrate that embryoid bodies (EBs) differentiated from talin1-null embryonic stem cells are defective in integrin adhesion complex assembly, epiblast elongation, and lineage differentiation. These defects are accompanied by a significant reduction in integrin β1 protein levels due to accelerated degradation through an MG-132-sensitive proteasomal pathway. Overexpression of integrin β1 or MG-132 treatment in mutant EBs largely rescues the phenotype. In addition, epiblast cells isolated from talin1-null EBs exhibit impaired cell spreading and focal adhesion formation. Transfection of the mutant cells with green fluorescent protein (GFP)-tagged wild-type but not mutant talin1 that is defective in integrin binding normalizes integrin β1 protein levels and restores focal adhesion formation. Significantly, cell adhesion and spreading are also improved by overexpression of integrin β1. All together, these results suggest that talin1 binding to integrin promotes epiblast adhesion and morphogenesis in part by preventing integrin β1 degradation.
Co-Microencapsulation of BMSCs and Mouse Pancreatic (3 Cells for Improving the Efficacy of Type I Diabetes Therapy

Introduction To overcome the shortcomings of pancreas transplantation and insulin injection treatment for type I diabetes, biocompatible materials were used to prepare alginate-chitosan-alginate microcapsules that co-encapsulated bone marrow mesenchymal stem cells and mouse pancreatic β cells to treat diabetic mice. Methods Blank alginate-chitosan-alginate (ACA) microcapsules and co-microencapsulated cells were prepared using a high-voltage electrostatic method and then characterized using an inverted microscope. Cell viability was evaluated using AO/EB staining. ELISA kit was used to detect insulin secretion. Peri-orbital blood samples were obtained from the mice for blood glucose determination every week for one month. Results After 28 days of in vitro culture, the secretion of insulin following co-microencapsulation was higher than that observed for microencapsulated beta-TC-6 cells alone. On the 28th day after transplantation, the blood glucose level was 6.86 mmol/L in the microencapsulated beta-TC-6 group. On the 14th day, the blood glucose level was 6.80 mmol/L in the co-microencapsulated BMSC/beta-TC-6 group, which was close to the normal blood glucose level of healthy mice. These results indicated that the efficacy in reducing blood glucose was better in the co-microencapsulated BMSC/beta-TC-6 group. Conclusions This primary study indicated that combining microencapsulation technology and co-culture of stem cells and somatic cells shows promise for the treatment of type I diabetes mellitus.
Ex vivo evaluation of a bipolar application concept for radiofrequency ablation.

BACKGROUND Bipolar radiofrequency ablation (RFA) can avoid complications such as thermal tissue damage, a possible consequence of monopolar RFA. However, basic studies about the dosage/effect relationship of bipolar systems are missing. This is systematic research on ex vivo beef livers to find which capacity parameters produce high energy in the tissue and achieve large lesion volumes. MATERIALS AND METHODS The active lengths 20, 30 and 40 mm of a bipolar, internally cooled applicator were studied. The tissue was fresh ex vivo beef liver. Five measurements each for each active applicator with a power between 10 and 50 W were conducted. RESULTS The best power for the 20 mm applicator was 15 W, since the highest achieved volume was 5,599+/-1,760 mm(3) and the highest amount of energy introduced to the tissue was 15+/-3 kJ. The best power for the 30 mm applicator was 20 W (volume 14,538+/-1,220 mm(3), energy 24+/-1 kJ). For the 40 mm applicator, the best power was 20 W, (volume 20,562+/-896 mm(3), energy 24+/-0 kJ). CONCLUSION The results of this study help clinicians determine which active length is required for the applicator and which presetting should be selected to achieve a defined coagulation volume size.
Community detection and role identification in directed networks: understanding the Twitter network of the care.data debate

With the rise of social media as an important channel for the debate and discussion of public affairs, online social networks such as Twitter have become important platforms for public information and engagement by policy makers. To communicate effectively through Twitter, policy makers need to understand how influence and interest propagate within its network of users. In this chapter we use graph-theoretic methods to analyse the Twitter debate surrounding NHS England's controversial care.data scheme. Directionality is a crucial feature of the Twitter social graph - information flows from the followed to the followers - but is often ignored in social network analyses; our methods are based on the behaviour of dynamic processes on the network and can be applied naturally to directed networks. We uncover robust communities of users and show that these communities reflect how information flows through the Twitter network. We are also able to classify users by their differing roles in directing the flow of information through the network. Our methods and results will be useful to policy makers who would like to use Twitter effectively as a communication medium.
‘There is Peace Here’

This article describes the relations between Hindus and Muslims in a small town in central Gujarat following the massive violence against the Muslim minority community throughout many parts of the state between February and May 2002. While the ‘communal divide’ has become more pronounced following the 2002 attacks in many parts of the state as well as in the town in which the research was conducted, concerted efforts are made by members of both communities as a means of re-imposing a sense of ‘everyday peace’. As such, normative discourses presenting the 2002 violence as an aberration with respect to the state of local communal relations in the town represent a collective strategy of containing the tension and mutual suspicion which remain constant undercurrents in daily life. This article, moreover, explores the ongoing caste– and class-based social networks and interactions underlying public declarations of ‘everyday peace’ which have played a central role in, if not averting violence altogether, discouraging the development of further communal segregation and division in the aftermath of the 2002 attacks in the town.
Arithmetic rules and numeral format

This research examined adults’ performance of simple addition and multiplication involving rule-based zero and one problems (N+0=N, N×1=N, N×0=0) presented as Arabic digits (5×1; 0+4) or written English number words (five×one; zero+four). The results showed that digits and number words differed in their capacity to activate the zero and one rules: With Arabic numerals, N×0=0 was relatively difficult compared to the other zero and one problems. This may be understood as an interference effect from the competing N+0=N and N×1=N rules, an interpretation supported by the high rate of N×0=N errors with Arabic stimuli. In contrast, with word stimuli, N×0 items were among the fastest items and yielded a relatively low rate of N×0=N errors. The results provide evidence that problem encoding mechanisms and central calculation mechanisms are interactive rather than strictly additive processes.
To report or not to report: a proposal on how to deal with altered test results in hemolytic samples

Abstract Preanalytically altered test results are a challenge every laboratory has to face. The release of such results may be to the harm of the patient by triggering wrong clinical decision making in monitoring or treatment. On the other hand, their deletion also might be to the harm of the patient by delaying the time to decision making as the exact value sometimes is not even necessary but rather an answer to the question “Is it raised or lowered”. Based on this dilemma and forced to produce laboratory values without any clinical information on the respective patient, laboratories have developed their own preferred way on how to deal with preanalytically altered test results. Some release the value with a comment, some reject the value with or without a comment and others again provide only general information about the hemolytic sample. To date there is no guideline or standardization to this postanalytical topic. Therefore, with this opinion paper, we want to start the scientific discussion on this important issue by providing one possible method to overcome the lack of clinical information which the laboratory would need to correctly decide whether or not to release an altered test result. We suggest providing the clinician with all the information on the hemolytic sample and its impact on the respective parameter needed to make his/her own decision on the usage of the respective test result. We believe that reporting a preanalytically altered laboratory value including a respective comment is preferable to not reporting it.
Impact of aging on muscle blood flow in chronic heart failure.

Chronic heart failure (CHF) is manifested principally in the elderly population. Therefore, to understand the causes of exercise intolerance in CHF patients, it is imperative to resolve the effects of aging on muscle blood flow (BF) in CHF. To address this issue, we determined the muscle BF response to submaximal treadmill exercise (20 m/min, 5% grade) in young (Y(CHF): 6-8 mo, 412 +/- 11 g, n = 11) and old (O(CHF): 27-29 mo, 494 +/- 10 g, n = 8) Fischer 344 x Brown Norway rats with similar degrees of myocardial infarction-induced left ventricular (LV) dysfunction [resting LV end-diastolic pressure: Y(CHF) = 24 +/- 2, O(CHF) = 22 +/- 2 mmHg; derivative of LV pressure over time: Y(CHF) = 5,168 +/- 285; O(CHF) = 5,050 +/- 165 mmHg/s; lung weight normalized to body weight: Y(CHF) = 9.14 +/- 0.72; O(CHF) = 8.21 +/- 0.29 mg/g (all P > 0.05)]. The exercising heart rate response was blunted in O(CHF) compared with Y(CHF) rats (Y(CHF) = 454 +/- 8, O(CHF) = 395 +/- 9 beats/min; P < 0.05). BF (radiolabeled microspheres) to the total hindlimb musculature and to each of the 28 individual muscles examined was similar between Y(CHF) and O(CHF) rats under resting conditions. During exercise, BF to five of the hindlimb muscles that normally possess a majority of slow-twitch oxidative and fast-twitch oxidative glycolytic muscle fibers increased significantly less (-25 to -42%) for O(CHF) compared with Y(CHF) rats. In contrast, BF to 14 of the hindlimb muscles that normally possess a majority of fast-twitch glycolytic muscle fibers was increased (+22 to +337%) for O(CHF) vs. Y(CHF) rats, which contributed to a greater mass-specific total hindlimb BF response in O(CHF) rats (Y(CHF) = 78 +/- 5, O(CHF) = 100 +/- 11 ml.min(-1). 100 g(-1); P < 0.05) and coincided with greater reductions in BF to the kidneys and splanchnic organs during exercise in O(CHF) vs. Y(CHF). In conclusion, there appears to be a profound age-related redistribution of BF from the highly oxidative to the highly glycolytic muscles of the hindlimb during exercise in O(CHF) compared with Y(CHF) rats. This phenomenon is qualitatively similar to that reported previously for healthy young and old rats.
Algorithms for dynamic closest pair and n-body potential fields

We present a general technique for dynamizing certain problems posed on point sets in Euclidean space for any fixed dimension d. This technique applies to a large class of structurally similar algorithms, presented previously by the authors, that make use of the well-separated pair decomposition. We prove efficient worst-case complexity for maintaining such computations under point insertions and deletions, and apply the technique to several problems posed on a set P containing n points. In particular, we show how to answer a query for any point x, that returns a constant-size set of points, a subset of which consists of all points in P that have x as a nearest neighbor. We then show how to use such queries to maintain the closest pair of points in P. We also show how to dynamize the fast multipole method, a technique for approximating the potential field of a set of point charges. All our algorithms use the algebraic model that is standard in computational geometry, and have worst-case deterministic O(log2 n) complexity for updates and queries.
A Five-Country Survey on Ethics Education in Preservice Teaching Programs

Despite a broad consensus on the ethical dimensions of the teaching profession, and long-standing efforts to align teacher education with wider trends in professional education, little is known about how teacher candidates are being prepared to face the ethical challenges of contemporary teaching. This article presents the results of an international survey on ethics content and curriculum in initial teacher education (ITE). Involving five Organisation for Economic Co-Operation and Development (OECD) countries—the United States, England, Canada, Australia, and the Netherlands—the study’s findings shed light on teacher educators’ perspectives on the contribution of ethics content to the education of future teachers and provide a snapshot of how well existing programs line up with their aspirations. The results showed that 24% of the ITE programs surveyed contain at least one mandatory stand-alone ethics course. The meaning of the results vis-à-vis opportunities for expanding ethics education in preservice teaching programs is also discussed.
A longitudinal investigation of medical student attitudes toward the medically indigent.

BACKGROUND Studies have shown that medical student attitudes decline as students progress through school. Controversy persists about the meaning and generalizability of these findings because studies have not been longitudinal and many were conducted prior to an influx of female students. Much of medical education occurs in academic medical center environments where disproportionate numbers of indigent (uninsured and underinsured) patients seek care. This study examined whether students' attitudes toward providing care to indigent clientele changed over time. PURPOSE To track longitudinal changes in attitudes toward providing care for the medically indigent of 1 cohort of medical students and to determine if gender differences existed and persisted over time. METHODS Students entering a Southwest medical school in 1989 participated in this study. Attitudes were compared using the Medical Students' Attitudes Toward the Underserved questionnaire, a self-report, attitudinal scale. RESULTS Student attitudes declined during the 1st year of school, remained fairly stable during Years 2 and 3, and declined further during Year 4. Analyses comparing 1st- and 4th-year students showed a decline in attitudes for men and women for each scale except Basic Services. Although both women's and men's attitudes declined, women's attitudes remained more favorable across the 4 years. CONCLUSIONS A clearer understanding of how female medical students sustain socially responsible attitudes throughout medical school may help educators develop support mechanisms for men and women that would promote an ethic of social responsibility and encourage students to work with indigent clientele.
Attention deficit hyperactivity symptoms and Internet addiction

Abstract The objective of this study was to evaluate the relationship between attention deficit‐hyperactivity/impulsivity symptoms and Internet addiction. In total, 535 elementary school students (264 boys, 271 girls; mean age, 11.0 ± 1.0 years) were recruited. The presence or severity of Internet addiction was assessed by the Young's Internet Addiction test. Parents and teachers of the children completed the DuPaul's attention deficit hyperactivity disorder (ADHD) rating scale (ARS; Korean version, K‐ARS) and Child Behavior Checklists. Children with the highest and lowest quartiles in K‐ARS scores were defined to be in ADHD and non‐ADHD groups, respectively. Five children (0.9%) met criteria for a definite Internet addiction and 75 children (14.0%) met criteria for a probable Internet addiction. K‐ARS scores had significant positive correlations with Young's Internet Addiction test scores. The Internet addiction group had higher total scores of K‐ARS and ADHD‐related subcategories in the Child Behavior Checklists than the non‐addiction group. The ADHD group had higher Internet addiction scores compared with the non‐ADHD group. Therefore, significant associations have been found between the level of ADHD symptoms and the severity of Internet addiction in children. In addition, current findings suggest that the presence of ADHD symptoms, both in inattention and hyperactivity‐impulsivity domains, may be one of the important risk factors for Internet addiction.
Fas gene promoter –670 polymorphism in gynecological cancer

Single-nucleotide polymorphism at −670 of Fas gene promoter (A/G) was examined in a total of 354 blood samples from normal healthy women and gynecological cancer patients. They consisted of 95 normal, 83 cervical, 108 endometrial, and 68 ovarian cancer cases. Eighty-three patients with cervical cancer had statistically higher frequency of GG genotype and G allele than 95 controls (P= 0.0353 and 0.0278, respectively). There was no significant difference in the genotype or allele prevalence between control subjects and endometrial or ovarian cancer patients. The Fas −670 GG genotype was associated with an increased risk for the development of cervical cancer (OR = 2.56, 95% CI = 1.08–6.10) compared with the AA genotype. The G allele also increased the risk of cervical cancer (OR = 1.60, 95% CI = 1.05–2.43) compared with the A allele. Germ-line polymorphism of Fas gene promoter −670 may be associated with the risk of cervical cancer in a Japanese population.
Femtosecond optical response of Y-Ba-Cu-O thin films: The dependence on optical frequency, excitation intensity, and electric current.

We have performed a series of femtosecond reflectivity experiments on various Y-Ba-Cu-O thin films at temperatures ranging from 12 to 300 K. In particular, the dependence of the optical response on probing laser frequency, pumping laser intensity, and bias electric current has been measured. Results obtained at room temperature provide quantitative information on the position of the Fermi level in films with different oxygen content. Systematic analysis of the measurements performed in the superconducting state indicates that the optical response associated with nonequilibrium properties of Y-Ba-Cu-O depends strongly on excitation intensity, sample thickness, and bias current. The results cannot be satisfactorily interpreted as the relaxation dynamics of quasiparticles, and a simple two-fluid model is shown to fail to explain data obtained under low laser excitation. Several tentative explanations are proposed, which provide a more comprehensive understanding of the transient optical response of Y-Ba-Cu-O.
Audio-Visual Redundancy and TV News Recall

This experimental study examines the effects of audio and visual redundancy on recall and story understanding in television news. College students viewed a series of voice-over news stories that varied in the amount of redundancy between the two channels and then responded to both auditory and visual recall measures. The results show higher auditory recall and story understanding in the high-redundancy condition than in the lower redundancy conditions. Visual recall shows the reverse pattern with higher recall scores in the lower redundancy conditions than in the high-redundancy condition.
An Empirical Investigation of Whether Authentic Web Sites Facilitate Intermediate-level French Language Students' Ability To Learn Culture

This investigation examined the effects of authentic Francophone web sites on cultural learning at the intermediate level. Fifty-four students "surfed" eight web sites. A pre-posttest design assessed long-term gains in culture and the learning of practices, products, and perspectives from exposure to a curriculum with Internet activities. Eight posttask tests measured the students' ability to retain information and to make inferences. A questionnaire assessed perceptions of learning. Results indicated a significant gain in cultural knowledge with posttest scores significantly higher than pretest scores. Students learned significantly more products than practices over the semester. On the short-answer and free-recall portions of the posttask tests, students' ability to make inferences or retain information did not improve significantly in either an advance organizer (AO) or a non-AO condition. The students believed that the web sites featured significantly more cultural products than practices. Results support using the Internet to teach culture and its potential to emphasize cultural products.
Social Divergence and Economic Performance

The paper introduces the concept of social divergence defined as the social barriers to communication and exchange between individuals and groups of individuals within a society. Social divergence is determined by the characteristics of a society including the distribution of income, ethnolinguistic diversity, religious diversity, educational distance, and other factors. The hypothesis is that social divergence reduces the degree of interaction between individuals that stimulates innovation and leads to the diffusion of productivity-enhancing ideas. Using a cross section of 31 developing countries, total factor productivity (TFP) is regressed on measures of social divergence. The results indicate that, separate from any effects due to factor accumulation, higher levels of social divergence result in lower levels of economic performance.
Cross-Resistance Between Dieldrin and Fipronil in German Cockroach (Dictyoptera: Blattellidae)

Abstract The toxicity of fipronil and dieldrin was determined in one susceptible laboratory strain and seven insecticide-resistant field-collected strains of Blattella germanica (L). The Zo960302 and Ga021001 strains were 1,270- and 2,030-fold resistant to dieldrin and 15- and 14-fold resistant to fipronil. The Su960304 and Od010803 strains were 15- and 13-fold resistant to dieldrin and two- and four-fold resistant to fipronil. Three strains showed no or a low level of resistance to dieldrin and fipronil. Crosses were performed between the susceptible strain Danish Pest Infestation Laboratory (DPIL)-SUS and the resistant strains Zo960302 and Su960304 and resistance to dieldrin and fipronil were intermediate compared with the susceptible and the resistant strains. Backcrosses to both of the parental strains showed cosegregation of dieldrin and fipronil resistance. The toxicity of dieldrin and fipronil was correlated when compared at LD50, and 93% of the observed variation in LD50 of fipronil can be ascribed to variation among predictions based on the value of LD50 of dieldrin. The frequency of the A302S substitution in the resistance to dieldrin (Rdl) gene in the highly dieldrin- and fipronil-resistant strains Zo960302 and Ga021001 and the moderately resistant Su960304 was 0.97, 1.0, and 0.38, respectively. We consider the connection between the frequency of the Rdl mutation and dieldrin and fipronil resistance a causal connection and not merely a coincidence.