Miracle-dz/UniFAR-FTUs huggingface dataset:
Disparities in How Parents Are Learning about the Human Papillomavirus Vaccine

Background: Differential access to basic health information may contribute to persistent cervical cancer disparities. We examined whether human papillomavirus (HPV) vaccine awareness, HPV knowledge, and use of information sources about the vaccine differ by sociodemographic characteristics associated with cervical cancer. Methods: Study participants (n = 889) were caregivers of adolescent girls ages 10 to 18 years living in southeastern North Carolina. Analyses simultaneously controlled for caregivers' gender, race, age, education, income, and rural residence. Results: Although most caregivers were aware of HPV (83%) and the HPV vaccine (82%), awareness differed by gender, race, education, and income. The largest differences were for race, with 87% of Whites versus 68% of African Americans having heard of the vaccine (P < 0.001). Caregivers correctly answered an average of 69% of questions on HPV, with differences by race and education. Most respondents heard of the HPV vaccine through drug company advertisements (83%) or broadcast media coverage (69%). African Americans were less likely than Whites to have heard about the vaccine from advertisements but more likely from a broadcast source (P < 0.05). Health care providers (88%) and the internet (65%) were the most favored sources for future information about the vaccine. Vaccine uptake was associated with awareness, knowledge, and media use. Discussion: Whereas drug company advertisements seem to play a central role in high HPV vaccine awareness, doctors and the internet are the preferred future “go to” sources for seeking out information. Communication-based interventions for caregivers from cervical cancer risk groups, especially African Americans, may need to use different communication channels and content. (Cancer Epidemiol Biomarkers Prev 2009;18(2):363–72)
Getting post-M&A integration mechanisms tuned in to technological relatedness and innovation synergy realisation

ABSTRACT Studies on Mergers and Acquisitions (M&A) typically relate innovation synergies to either context characteristics or post-M&A integration. There is little research on how to tune the relevant practices to the benefit of realising specific innovation synergies. It is the purpose of this paper to develop a conceptual framework on innovation synergy realisation in large M&As, that relates the following components: (1) strategic M&A characteristics; and (2) post-M&A integration mechanisms; to (3) innovation synergy realisation. The research explored how different innovation synergies were achieved in nine large medium-tech and high-tech M&As in the life sciences. From this case studies research, it turns out that higher degrees of technological relatedness allow for the realisation of more types of innovation synergy, brought about by the more demanding integration mechanisms structural linking and process re-design.
Ancient Clam Gardens Increased Shellfish Production: Adaptive Strategies from the Past Can Inform Food Security Today

Maintaining food production while sustaining productive ecosystems is among the central challenges of our time, yet, it has been for millennia. Ancient clam gardens, intertidal rock-walled terraces constructed by humans during the late Holocene, are thought to have improved the growing conditions for clams. We tested this hypothesis by comparing the beach slope, intertidal height, and biomass and density of bivalves at replicate clam garden and non-walled clam beaches in British Columbia, Canada. We also quantified the variation in growth and survival rates of littleneck clams (Leukoma staminea) we experimentally transplanted across these two beach types. We found that clam gardens had significantly shallower slopes than non-walled beaches and greater densities of L. staminea and Saxidomus giganteus, particularly at smaller size classes. Overall, clam gardens contained 4 times as many butter clams and over twice as many littleneck clams relative to non-walled beaches. As predicted, this relationship varied as a function of intertidal height, whereby clam density and biomass tended to be greater in clam gardens compared to non-walled beaches at relatively higher intertidal heights. Transplanted juvenile L. staminea grew 1.7 times faster and smaller size classes were more likely to survive in clam gardens than non-walled beaches, specifically at the top and bottom of beaches. Consequently, we provide strong evidence that ancient clam gardens likely increased clam productivity by altering the slope of soft-sediment beaches, expanding optimal intertidal clam habitat, thereby enhancing growing conditions for clams. These results reveal how ancient shellfish aquaculture practices may have supported food security strategies in the past and provide insight into tools for the conservation, management, and governance of intertidal seascapes today.
Breast Cancer Classification in Histopathological Images using Convolutional Neural Network

Computer based analysis is one of the suggested means that can assist oncologists in the detection and diagnosis of breast cancer. On the other hand, deep learning has been promoted as one of the hottest research directions very recently in the general imaging literature, thanks to its high capability in detection and recognition tasks. Yet, it has not been adequately suited to the problem of breast cancer so far. In this context, I propose in this paper an approach for breast cancer detection and classification in histopathological images. This approach relies on a deep convolutional neural networks (CNN), which is pretrained on an auxiliary domain with very large labelled images, and coupled with an additional network composed of fully connected layers. The network is trained separately with respect to various image magnifications (40x, 100x, 200x and 400x). The results presented in the patient level achieved promising scores compared to the state of the art methods.
The effect of thermal dose on hyperthermia-mediated inhibition of DNA repair through homologous recombination

Hyperthermia has a number of biological effects that sensitize tumors to radiotherapy in the range between 40-44 °C. One of these effects is heat-induced degradation of BRCA2 that in turn causes reduced RAD51 focus formation, which results in an attenuation of DNA repair through homologous recombination. Prompted by this molecular insight into how hyperthermia attenuates homologous recombination, we now quantitatively explore time and temperature dynamics of hyperthermia on BRCA2 levels and RAD51 focus formation in cell culture models, and link this to their clonogenic survival capacity after irradiation (0-6 Gy). For treatment temperatures above 41 °C, we found a decrease in cell survival, an increase in sensitization towards irradiation, a decrease of BRCA2 protein levels, and altered RAD51 focus formation. When the temperatures exceeded 43 °C, we found that hyperthermia alone killed more cells directly, and that processes other than homologous recombination were affected by the heat. This study demonstrates that optimal inhibition of HR is achieved by subjecting cells to hyperthermia at 41-43 °C for 30 to 60 minutes. Our data provides a guideline for the clinical application of novel combination treatments that could exploit hyperthermia's attenuation of homologous recombination, such as the combination of hyperthermia with PARP-inhibitors for non-BRCA mutations carriers.
Socioeconomic Differences and Trends in the Place of Death among Elderly People in China

China is facing a dramatic aging of its population. Little is known about the factors that influence the place of death and the trends in the place of death for elderly people in China. The purposes of this study were: (1) to examine the impact of the socioeconomic status (SES) on place of death for elderly Chinese residents; and (2) to assess temporal trends in the place of death over the last 15 years. Data were derived from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) (1998–2012). Place-of-death as an outcome was dichotomized into either death at home or death outside the home. Logistic regression analyses were used to examine the impact of SES on place of death. The results showed that, of the 23,098 deaths during the study period, 87.78% occurred at home. The overall trend in home death has increased since 2005. SES was shown to be an important factor affecting place of death. The elderly with higher SES were more likely to die where health resources were concentrated, i.e., in a hospital or other type of institution. Our finding suggests that the trend towards a greater emphasis on death at home may call for the development of more supportive home care programs in China. Our finding also suggests that the socioeconomic differences in the place of death may be related to the availability of or access to health care services.
Escape from X chromosome inactivation is an intrinsic property of the Jarid1c locus

Although most genes on one X chromosome in mammalian females are silenced by X inactivation, some “escape” X inactivation and are expressed from both active and inactive Xs. How these escape genes are transcribed from a largely inactivated chromosome is not fully understood, but underlying genomic sequences are likely involved. We developed a transgene approach to ask whether an escape locus is autonomous or is instead influenced by X chromosome location. Two BACs carrying the mouse Jarid1c gene and adjacent X-inactivated transcripts were randomly integrated into mouse XX embryonic stem cells. Four lines with single-copy, X-linked transgenes were identified, and each was inserted into regions that are normally X-inactivated. As expected for genes that are normally subject to X inactivation, transgene transcripts Tspyl2 and Iqsec2 were X-inactivated. However, allelic expression and RNA/DNA FISH indicate that transgenic Jarid1c escapes X inactivation. Therefore, transgenes at 4 different X locations recapitulate endogenous inactive X expression patterns. We conclude that escape from X inactivation is an intrinsic feature of the Jarid1c locus and functionally delimit this escape domain to the 112-kb maximum overlap of the BACs tested. Additionally, although extensive chromatin differences normally distinguish active and inactive loci, unmodified BACs direct proper inactive X expression patterns, establishing that primary DNA sequence alone, in a chromosome position-independent manner, is sufficient to determine X chromosome inactivation status. This transgene approach will enable further dissection of key elements of escape domains and allow rigorous testing of specific genomic sequences on inactive X expression.
Two-Dimensional Whitening Reconstruction for Enhancing Robustness of Principal Component Analysis

Principal component analysis (PCA) is widely applied in various areas, one of the typical applications is in face. Many versions of PCA have been developed for face recognition. However, most of these approaches are sensitive to grossly corrupted entries in a 2D matrix representing a face image. In this paper, we try to reduce the influence of grosses like variations in lighting, facial expressions and occlusions to improve the robustness of PCA. In order to achieve this goal, we present a simple but effective unsupervised preprocessing method, two-dimensional whitening reconstruction (TWR), which includes two stages: 1) A whitening process on a 2D face image matrix rather than a concatenated 1D vector; 2) 2D face image matrix reconstruction. TWR reduces the pixel redundancy of the internal image, meanwhile maintains important intrinsic features. In this way, negative effects introduced by gross-like variations are greatly reduced. Furthermore, the face image with TWR preprocessing could be approximate to a Gaussian signal, on which PCA is more effective. Experiments on benchmark face databases demonstrate that the proposed method could significantly improve the robustness of PCA methods on classification and clustering, especially for the faces with severe illumination changes.
Differential Regulation of Human Platelet Responses by cGMP Inhibited and Stimulated cAMP Phosphodiesterases

Summary Platelets contain two cAMP phosphodiesterases (PDEs) which regulate intracellular cAMP levels, cGMP-inhibited cAMP PDE (PDE3A) and cGMP-stimulated PDE (PDE2A). Using the PDE3 inhibitor, milrinone and the PDE2 inhibitor, erythro-9-(2-hydroxyl-3-nonyl)adenine (EHNA), we have explored the contribution of each PDE to the regulation of platelet function. Inhibition of PDE2 resulted in higher levels of intracellular cAMP than inhibition of PDE3A suggesting this PDE may be the more important regulator of cAMP in human platelets. However, a concentration-dependent inhibition of agonist-induced aggregation was observed with milrinone while little effect was seen with EHNA. In addition, we observed a concentration-dependent inhibition in the increase of intracellular Ca2+ with PDE3 inhibition and significantly less with PDE2 inhibition. PDE3 inhibition also resulted in a concentration-dependent increase in cAMP-mediated phosphorylation of the vasodilator-stimulated phospho-protein (VASP) whereas there was no significant increase with PDE2 inhibition. In each of these experiments, synergism was noted with the combination of milrinone and EHNA. These results suggest that cAMP pools may be localized and the various PDEs regulate specific pools. These data also suggest that inhibitors of PDE3A may be more effective antiplatelet agents.
Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis

Osteoporosis is a serious public health problem and icariin (ICA) is the active component of the Epimedium sagittatum, a traditional Chinese medicinal herb. The present study aimed to investigate the effects and underlying mechanisms of ICA as a potential therapy for osteoporosis. Calvaria osteoblasts were isolated from newborn rats and treated with ICA. Cell viability, apoptosis, alkaline phosphatase activity and calcium deposition were analyzed. Bioinformatics analyses were performed to identify differentially expressed proteins (DEPs) in response to ICA treatment. Western blot analysis was performed to validate the expression of DEPs. ICA administration promoted osteoblast viability, alkaline phosphatase activity, calcium deposition and inhibited osteoblast apoptosis. Secretome analysis of ICA-treated cells was performed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A total of 56 DEPs were identified, including serpin family F member 1 (PEDF), protein disulfide isomerase family A, member 3 (PDIA3), nuclear protein, co-activator of histone transcription (NPAT), c-Myc and heat shock protein 70 (HSP70). These proteins were associated with signaling pathways, including Fas and p53. Bioinformatics and western blot analyses confirmed that the expression levels of the six DEPs were upregulated following ICA treatment. These genes may be directly or indirectly involved in ICA-mediated osteogenic differentiation and osteogenesis. It was demonstrated that ICA treatment promoted osteogenesis by modulating the expression of PEDF, PDIA3, NPAT and HSP70 through signaling pathways, including Fas and p53.
Magnetically-focusing biochip structures for high-speed active biosensing with improved selectivity

We report a magnetically-focusing biochip structure enabling a single layered magnetic trap-and-release cycle for biosensors with an improved detection speed and selectivity. Here, magnetic beads functionalized with specific receptor molecules were utilized to trap target molecules in a solution and transport actively to and away from the sensor surfaces to enhance the detection speed and reduce the non-specific bindings, respectively. Using our method, we demonstrated the high speed detection of IL-13 antigens with the improved detection speed by more than an order of magnitude. Furthermore, the release step in our method was found to reduce the non-specific bindings and improve the selectivity and sensitivity of biosensors. This method is a simple but powerful strategy and should open up various applications such as ultra-fast biosensors for point-of-care services.
Explaining financial market facts: the importance of incomplete markets and transaction costs

In this article, I suggest that incomplete markets and transaction costs are crucial for explaining the high equity premium and the low risk-free rate. I first demonstrate the failure of the complete frictionless markets model in explaining these return puzzles and then show how introducing incomplete markets and transaction costs can lead to success. Additionally, I explain how these features lead to predictions concerning individual consumptions, wealths, portfolios, and asset market transactions that are in better agreement with the facts than the predictions of the complete frictionless markets model.
Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo

The adult blood system is established by hematopoietic stem cells (HSCs), which arise during development from an endothelial-to-hematopoietic transition of cells comprising the floor of the dorsal aorta. Expression of aortic runx1 has served as an early marker of HSC commitment in the zebrafish embryo, but recent studies have suggested that HSC specification begins during the convergence of posterior lateral plate mesoderm (PLM), well before aorta formation and runx1 transcription. Further understanding of the earliest stages of HSC specification necessitates an earlier marker of hemogenic endothelium. Studies in mice have suggested that GATA2 might function at early stages within hemogenic endothelium. Two orthologs of Gata2 exist in zebrafish: gata2a and gata2b. Here, we report that gata2b expression initiates during the convergence of PLM, becoming restricted to emerging HSCs. We observe Notch-dependent gata2b expression within the hemogenic subcompartment of the dorsal aorta that is in turn required to initiate runx1 expression. Our results indicate that Gata2b functions within hemogenic endothelium from an early stage, whereas Gata2a functions more broadly throughout the vascular system. Highlighted article: Gata2b marks a distinct population of embryonic endothelial cells that gives rise to hematopoietic stem cells and is required for the hemogenic potential of these cells.
Integrin-substrate interactions underlying shear-induced inhibition of the inflammatory response of endothelial cells

Summary Conditioning of endothelial cells by shear stress suppresses their response to inflammatory cytokines. We questioned whether signalling through different integrin-matrix interactions, previously associated with the pathogenic effects of disturbed flow, supported the anti-inflammatory action of steady shear. Primary human endothelial cells were cultured on different substrates and exposed to shear stress (2.0Pa) for varying periods before stimulation with tumour necrosis factor-α (TNF). Shear-conditioning inhibited cytokine-induced recruitment of flowing neutrophils. However, the effect was similar for culture on collagen, laminin or fibronectin, even when seeding was reduced to 2hours, and shear to 3hours before TNF treatment (to minimise deposition of endothelial matrix). Nevertheless, in short- or longer- term cultures, reduction in expression of β1-integrin (but not β3-integrin) using siRNA essentially ablated the effect of shear-conditioning on neutrophil recruitment. Studies of focal adhesion kinase (FAK) phosphorylation, siRNA against FAK and a FAK-inhibitor (PF573228) indicated that FAK activity was an essential component downstream of β1-integrin. In addition, MAP-kinase p38 was phosphorylated downstream of FAK and also required for functional modification. Mechanotransduction through β1-integrins, FAK and p38 is required for anti-inflammatory effects of steady shear stress. Separation of the pathways which underlie pathological versus protective responses of different patterns of flow is required to enable therapeutic modification or mimicry, respectively.
Strengths and Weaknesses of the Young Black Men, Masculinities, and Mental Health (YBMen) Facebook Project

The Young Black Men, Masculinities, and Mental Health (YBMen) project is a Facebook-based intervention that provides mental health education and social support to young Black men. The YBMen project was created to better understand and address the pressures and needs of young Black men, particularly with regard to issues related to their conceptualization of masculinity and mental health. Black men from a 2-year liberal arts college in the Midwest (United States) enrolled in the YBMen pilot project. The purpose of this study is to report what participants in the YBMen pilot project liked and disliked about the intervention, along with their suggestions for improvement. Qualitative results from the 8 Black men who actively participated in the YBMen Facebook intervention and completed the postintervention interview are reported. A systematic analysis identified 9 subthemes that described participants’ reactions to different components and characteristics of the Facebook intervention. Results indicated that opportunities for relationship building and connectivity, coupled with engaging popular culture references used in the intervention encouraged young Black men to actively participate in the YBMen Facebook intervention. The YBMen project has potential to improve the health and well-being of young Black men by providing nontraditional resources that are easily accessible, culturally sensitive, and gender-specific. Implications of the YBMen project as an effective Internet-based program that promotes mental health and increases social support among young Black men are discussed.
Effect of Water and Nitrogen Management on Water Productivity and Nitrogen Use Efficiency of Wheat in a Semi-arid Environment

A field experiment was carried out during 2009-11 on a sandy loam soil of IARI, New Delhi on wheat with four levels of irrigation (rainfed, irrigations to replenish 30, 60 and 100% moisture deficit from field capacity) as main plot factors and four levels of nitrogen (0, 30, 60 and 120 kg N/ha) as subplot factors in a split plot design. The soil moisture storage in the profile showed inverse relation with the nitrogen levels whereas direct relationship with the irrigation levels. The water use vs. wheat yield relation showed that 58 % variation in grain yield of wheat could be explained by water use. The grain yield showed increasing trend with increase in irrigation and nitrogen levels. The water productivity (WP) and economic water productivity (EWP) increased with increase in irrigation level up to 60 % SMD and thereafter it decreased. However, both WP and EWP showed increasing trend with increase in nitrogen levels. The agronomic nitrogen use efficiency (ANUE) and partial factor productivity of nitrogen (PFPN) increased with increase in irrigation levels, but with increase in nitrogen levels both ANUE and PFPN decreased. There was significant positive interaction between irrigation and nitrogen levels with respect to grain yield, water productivity and nitrogen use efficiency of wheat. Thus from this study, it may be concluded that wheat may be grown with irrigation to replenish 60% SMD to field capacity and at 120 kg N/ha to achieve higher water productivity and nitrogen use efficiency without any significant reduction in crop yield S. Pradhan et al 728 and thus resulting in saving of irrigation water in sandy loam soils of semiarid region of North India.