Miracle-dz/UniFAR-FTUs huggingface dataset:
Capnocytophaga sp. Isolated from a Cat with Chronic Sinusitis and Rhinitis

ABSTRACT A Capnocytophaga sp. was inadvertently isolated from a cat with chronic sinusitis and rhinitis when cytopathic effects were observed in Crandall-Reese feline kidney cells that had been inoculated with oropharyngeal swab samples. Although Capnocytophaga spp. are of considerable zoonotic importance, their clinical relevance for dogs or cats has not been established. However, failure to do so may be attributed to the infrequent use of specialized isolation techniques that are required to grow Capnocytophaga spp. To our knowledge, successful isolation of these organisms from a cat with nasopharyngeal disease has not been reported.
Palmitoylation of Human EndothelinB

By site-directed mutagenesis, three cysteine residues (amino acids 402, 403, and 405) in the carboxyl terminus of human endothelinB (ETB) were identified as potential palmitoylation sites. Substitutions of all of the three cysteine residues with serine gave an unpalmitoylated mutant, C2S/C3S/C5S. When expressed in Chinese hamster ovary cells, C2S/C3S/C5S was localized on the cell surface, retained high affinities to ET-1 and ET-3, and was rapidly internalized when bound to the ligand. However, unlike the wild-type ETB, C2S/C3S/C5S transmitted neither an inhibitory effect on adenylate cyclase nor a stimulatory effect on phospholipase C, indicating a critical role of palmitoylation in the coupling with G proteins, regardless of the G protein subtypes. Truncation of the carboxyl terminus including Cys403/Cys405 gave a deletion mutant Δ403 that was palmitoylated on Cys402 and lacked the carboxyl terminus downstream to the palmitoylation site. Δ403 did transmit a stimulatory effect on phospholipase C via a pertussis toxin-insensitive G protein but it failed to transmit an inhibitory effect on adenylate cyclase. These results indicated a differential requirement for the carboxyl terminus downstream to the palmitoylation site in the coupling with G protein subtypes, i.e. it is required for the coupling with Gi but not for that with Gq.
Voltage-source active power filter based on multilevel converter and ultracapacitor DC link

A new topology for active power filters (APF) using an 81-level converter is analyzed. Each phase of the converter is composed of four three-state converters, all of them connected to the same capacitor dc link voltage and their output connected in series through output transformers. The main advantages of this kind of converter are the negligible harmonic distortion obtained and the very low switching frequency operation. The single-phase equivalent circuit is analyzed and their governing equations derived. The dc link voltage control, based on manipulating the converter's voltage phase, is analyzed together with the circuit's characteristics that determine the capability to draw or deliver active and reactive current. Simulation results for this application are compared with conventional pulsewidth-modulated (PWM) converters, showing that this filter can compensate load current harmonics, keeping better-quality sinusoidal currents from the source. The simulated configuration uses a 1-F ultracapacitor in the dc link, making it possible to store energy and deliver it during short voltage dips. This is achieved by applying a modulation control to maintain a stable ac voltage during dc voltage drops. A prototype of the filter was implemented and tested, and the obtained current waveforms showed to be as good as expected.
Output feedback control for uncertain singularly perturbed systems

In this paper, the problem of quadratic stabilizability of uncertain singularly perturbed systems by dynamic output feedback is addressed. Using a time-scale decomposition. two smaller-order ε-independent quadratic stabilizability subproblems are solved and it is shown that when ε is sufficiently small the combination of the corresponding results yields a quadratic stabilizing controller for the full order uncertain system, the controller being singularly perturbed itself with the same parameter ε as that of the system.
Neural coding merges sex and habitat chemosensory signals in an insect herbivore

Understanding the processing of odour mixtures is a focus in olfaction research. Through a neuroethological approach, we demonstrate that different odour types, sex and habitat cues are coded together in an insect herbivore. Stronger flight attraction of codling moth males, Cydia pomonella, to blends of female sex pheromone and plant odour, compared with single compounds, was corroborated by functional imaging of the olfactory centres in the insect brain, the antennal lobes (ALs). The macroglomerular complex (MGC) in the AL, which is dedicated to pheromone perception, showed an enhanced response to blends of pheromone and plant signals, whereas the response in glomeruli surrounding the MGC was suppressed. Intracellular recordings from AL projection neurons that transmit odour information to higher brain centres, confirmed this synergistic interaction in the MGC. These findings underscore that, in nature, sex pheromone and plant odours are perceived as an ensemble. That mating and habitat cues are coded as blends in the MGC of the AL highlights the dual role of plant signals in habitat selection and in premating sexual communication. It suggests that the MGC is a common target for sexual and natural selection in moths, facilitating ecological speciation.
Antiremodeling Effects of Iloprost and the Dual-Selective Phosphodiesterase 3/4 Inhibitor Tolafentrine in Chronic Experimental Pulmonary Hypertension

Abstract— Severe pulmonary hypertension is a disabling disease with high mortality. We investigated acute and chronic effects of iloprost, a long-acting prostacyclin analogue, and the dual-selective phosphodiesterase 3/4 inhibitor tolafentrine in monocrotaline-induced pulmonary hypertension in rats. Twenty-eight and 42 days after administration of the alkaloid, right ventricular systolic pressure increased from 25.8±2.0 to 62.9±3.4 and 70.5±7.4 mm Hg, with concomitant decline in cardiac index, central venous oxygen saturation, and arterial oxygenation. Marked right heart hypertrophy was demonstrated by the strongly elevated ratio of right ventricle/left ventricle plus septum weight, and massive thickening of the precapillary artery smooth muscle layer was shown histologically. Western blot analysis demonstrated increased levels of matrix metalloproteinases (MMPs) -2 and -9 and increased gelatinolytic activities in isolated pulmonary arteries. In these animals, both intravenous iloprost and tolafentrine displayed characteristic features of pulmonary vasodilators. When chronically infused from days 14 to 28, both agents significantly attenuated all monocrotaline-induced hemodynamic and gas exchange abnormalities as well as right heart hypertrophy. Full normalization of all variables including right ventricle size was achieved on combined administration of both agents during this period. This was also true for MMP-2 and MMP-9 expression and activity. Moreover, when iloprost plus tolafentrine was used for late therapeutic intervention, with infusion from days 28 to 42 after full establishment of severe pulmonary hypertension and cor pulmonale, hemodynamic, gas exchange, and cardiac and pulmonary vascular remodeling changes were significantly reversed. We conclude that the combined administration of iloprost and a dual-selective phosphodiesterase 3/4 inhibitor prevents and reverses the development of pulmonary hypertension and cor pulmonale in response to monocrotaline in rats. This regimen may therefore offer a possible antiremodeling therapy in severe pulmonary hypertension.
Salmonella enterica: a surprisingly well-adapted intracellular lifestyle

The infectious intracellular lifestyle of Salmonella enterica relies on the adaptation to nutritional conditions within the Salmonella-containing vacuole (SCV) in host cells. We summarize latest results on metabolic requirements for Salmonella during infection. This includes intracellular phenotypes of mutant strains based on metabolic modeling and experimental tests, isotopolog profiling using 13C-compounds in intracellular Salmonella, and complementation of metabolic defects for attenuated mutant strains towards a comprehensive understanding of the metabolic requirements of the intracellular lifestyle of Salmonella. Helpful for this are also genomic comparisons. We outline further recent studies and which analyses of intracellular phenotypes and improved metabolic simulations were done and comment on technical required steps as well as progress involved in the iterative refinement of metabolic flux models, analyses of mutant phenotypes, and isotopolog analyses. Salmonella lifestyle is well-adapted to the SCV and its specific metabolic requirements. Salmonella metabolism adapts rapidly to SCV conditions, the metabolic generalist Salmonella is quite successful in host infection.
Searching the schizophrenic brain for temporal lobe deficits: a systematic review and meta-analysis

Background. Several empirical studies have found temporal lobe impairments in many patients with schizophrenia. The strength and consistency of this evidence, however, has not been evaluated and synthesized quantitatively. Hence, we ask to what extent are temporal cortices really defective in schizophrenia? Methods. Meta-analytical methods were used to determine the magnitude of evidence in support of structural and physiological temporal–hippocampal system deficits in schizophrenia. We report effect sizes from studies since 1980 that used structural (CT, MRI) and functional (SPECT, PET) neuroimaging methods. Results. Both structural and functional imaging literatures are distinguished by heterogeneity whereby most patients show normative temporal function and structure, a minority shows diminished values and some patients demonstrate augmented function and structure rather than a deficit. Conclusions. The findings are hard to incorporate within single disease models that propose major involvement of the temporal system in schizophrenia, at least at the degree of resolution obtained with current imaging technology.
Migrations and Multiplications of Bursaphelenchus xylophilus and B. mucronatus in Pinus thumbergii in Relation to Their Pathogenicity

To evaluate the mechanisms of pathogenicity and non-pathogenicity of Bursaphelenchus xylophilus and B. mucronatus isolated in South Korea, we used 4-year-old P. thunbergii seedlings and 20-cm long one-year-old stem cuttings of 5-year-old Pinus thunbergii, and studied distributions and multiplications of pine wood nematodes after inoculation. The distributions of B. xylophilus in the 20-cm pine stem cuttings were not significantly different from that of B. mucronatus. Conversely, the proliferation rate of B. xylophilus on mycelial mats of Botrytis cinerea was significantly different from that of B. mucronatus. The study using 4-year-old P. thunbergii seedlings also showed that B. mucronatus can migrate to distal portions of the pine seedlings the same as B. xylophilus, but the populations of B. xylophilus remaining in the pine seedlings were relatively larger than those of B. mucronatus. Therefore, we concluded that the pathogenicity of B. xylophilus could be strongly influenced by its ability to multiply.
Micromonas , a small pigmented flagellate, predominates the nanoflagellate and photosynthetic picoeukaryote communities in the northern South China Sea

Abstract A small pigmented flagellate, Micromonas, is prevalently distributed in coastal and pelagic waters. However, there have been few studies conducted to quantify their abundance in the marginal seas of the Northwest Pacific Ocean. In this study, we used fluorescent in situ hybridization with tyramide signal amplification (TSA‐FISH) to reveal the spatial distribution of Micromonas in the northern South China Sea (SCS). On average, the abundance of Micromonas was 317 cells mL−1, with the average proportions in the nanoflagellates (NF) and photosynthetic picoeukaryotes (PPE) communities being 10.94% and 15.39%, respectively. This indicates a wide distribution and dominance of this genus in the studied area. The relationships between Micromonas abundance and various environmental factors suggested that biotic correlations play more important roles than physicochemical filtering on Micromonas assemblage. This may indicate a broad environmental adaptation spectrum of this genus through its flexibility in terms of resource acquisition strategies. In summary, this study provides insight into the spatial distribution pattern of Micromonas and highlights its crucial contribution to the composition of NFs and PPE communities, which rely on biological interaction to respond to the changing environmental conditions in the northern SCS.
Diode laser ignition of high explosives and pyrotechnics

Thermal ignition of the explosive CP (2-(5-cyanotetrazolato)pentaamine cobalt (III) perchlorate) and of the pyrotechnic Ti/KCl0/sub 4/ has been achieved using a (GaAl)As diode laser that emitted 1 watt of optical power from the end of a 100/minus/. mu.m core fiber optic to which it was coupled. The wavelength of the emitted light was near 820 nm. Approximate ignition thresholds were obtained for pulse widths of between 2 and 10 ms. For a 10/minus/ms pulse width, pure CP required /approximately/6.7 mJ of energy for ignition whereas pure Ti/KC10/sub 4/ required only /approximately/3 mJ. Dopants such as carbon black, graphite and a laser dye were added in small concentrations to enhance light absorption and thereby reduce the ignition theshold. Their effects as well as those of powder density, particle size and spot size of the incident light on ignition threshold are described. 9 refs., 7 figs., 3 tabs.
A model of pathological oscillations in the basal ganglia and deep brain stimulation in parkinson’s disease

A model of pathological oscillatory activity within the basal ganglia in Parkinson’s disease is presented. Synchronous oscillatory activity of the subthalamic nucleus (STN) and globus pallidus external (GPe) is simulated at both the parkinsonian tremor (3-9) Hz and beta (15-30) Hz frequencies. The model extends previous models to incorporate cortical inputs to the subthalamic nucleus through the ‘hyperdirect’ pathway, changes in coupling between STN neurons due to dopamine depletion and the plateau potential generating capacity of STN neurons. The effect of deep brain stimulation (DBS) across a range of frequencies on the oscillatory activity is examined. High frequency DBS attenuates synchronous oscillatory activity within the STN-GPe network at both the tremor and beta frequencies. The efficacy of the DBS input in quenching the pathological oscillations is shown to vary systematically with the frequency, pulse width and amplitude of the applied stimulus.
Pushing an Object Considering the Hand Reflect Forces by Humanoid Robot in Dynamic Walking

This paper discusses pushing a heavy object by a humanoid robot. We modify the whole body motion considering the hand reflecting forces for the walking pattern of a humanoid robot. By assuming that linear and angular momentum of a humanoid robot are calculated, we define the projection of the Center of the Mass(CoM) as” Dynamically Complemental Zero Moment Point(DCZMP)”, when external forces act to the end-effectors. We propose a new method using the DCZMP for the modification control of CoM position with balancing control. The robot can keep the dynamical balance considering the DCZMP and the walking velocity in both single and double support phase. In addition, for controlling pushing force, we implement an impedance controller with the manipulation and walking velocity controller. The effectiveness of the proposed method is confirmed by simulations and experiments.
Usefulness of Probiotics for Neonates

In humans there are a multitude of site-specific communities of bacteria localized on the skin, mucosal surfaces, and in the intestinal tract [1,2]. The total number of prokaryotic cells is estimated to be around 1014, ten times more than the number of eukaryotic cells. These microbial communities interact extensively with the host, a process which is crucial for host development and homeostasis. Most of the microbiota is located in the gastrointestinal (GI) tract, and progressively increase in number from the jejunum to the colon. In the colon, the levels of bacteria are as high as 1011 microorganisms per gram of luminal content with a very wide diversity. The composition of gut microbial communities was originally known through culture-based studies, which estimated that 400 to 500 different species are present in the adult human intestinal tract [3]. Through the most recent culture-independent analyses, gut microbiota is thought to comprise up to 1000 bacterial species per individual and over 5000 species in total [4]. The gut microbiota is dominated by only four phyla, i.e. Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria, although there are more than 50 bacterial phyla on Earth [1].
Nitroxide-Mediated Controlled Radical Copolymerization of α-Trifluoromethylstyrenes with Styrenes

Fluorinated polymers are important materials in everyday life; however, most monomers of widely used fluoropolymers are gaseous, and their polymerization is difficult in an ordinary laboratory. Therefore, partially fluorinated polymers have recently been reported. As an easy-to-handle fluorine-containing monomer, α-trifluoromethylstyrene (TFMST) can be used to produce partially fluorinated polymers with trifluoromethyl groups in the main chain; however, TFMST does not homopolymerize, and there are limited reports on its copolymerization with styrene (ST). In this study, we applied the controlled radical polymerization method, which is effective for the polymerization of ST, to the copolymerization of TFMST and ST. We also showed that nitroxide-mediated polymerization is effective. The content ratio of TFMST in the TFMST–ST copolymer can be controlled between 10% and 40% by changing its monomer ratio. Additionally, the polymerization of TFMST and ST with substituents was performed to increase structural variations. The thermal stability as well as water and oil repellency of the synthesized polymers with different composition ratios and substituents were also evaluated.
How and When Review Length and Emotional Intensity Influence Review Helpfulness: Empirical Evidence from Epinions.com

Although longer reviews are generally considered more helpful, no research has investigated whether “the more the better” also applies to the expression of emotions. This paper explores the distinct effects of review length and emotional intensity. We propose that, in contrast to review length, the intensity of emotions has a negative effect on review helpfulness, and that this effect only applies to positive emotions. Additionally, drawing on elaboration likelihood model and the literature on the social functions of emotions, we predict that the respective effects of review length and emotional intensity are moderated by reviewer trustworthiness and the difficulty of reading review content. To test these hypotheses, we collected a rich data set from Epinions.com + a leading provider of consumer revie ws. Our findings reveal the importance of taking the intensity of emotions into consideration when evaluating review helpfulness, and the results carry important practical implications.
Lymphadenitis associated with cat-scratch disease simulating a neoplasm: Imaging findings with histopathological associations

The lymphadenitis associated with cat-scratch disease (CSD) is often confused with neoplasms by a number of radiologists and clinicians, and consequently, unnecessary invasive procedures or surgeries are performed. In the present study, the contrast-enhanced computed tomography (CT) and magnetic resonance imaging (MRI) findings of 10 patients (6 men and 4 women) with clinically and pathologically confirmed lymphadenitis associated with CSD were retrospectively analyzed (CT in 3 patients, MRI in 6 patients, and CT and MRI in 1 patient) at The Second Affiliated Hospital of Zhejiang University School of Medicine (Hangzhou, China) between January 2007 and November 2014. As a result, 17 enlarged lymph nodes were identified in 10 cases. The 5 nodes identified by CT scan exhibited relatively inhomogeneous isodensity to muscle, with patchy low density in the center. All 14 nodes identified by MRI scan exhibited homogeneous or heterogeneous isointensity to muscle or slightly increased intensity compared with that of muscle on T1-weighted images (T1WI), and homogeneous or heterogeneous hyperintensity on fat-suppressed T2WI. Following enhancement, all 17 enlarged lymph nodes associated with CSD demonstrated the following 3 different enhancement patterns: Moderate homogeneous enhancement (n=8), which was associated with histologically identified early disease stage; marked heterogeneous enhancement with no enhancement of the necrotic areas (n=4), and heterogeneous enhancement with progressively ‘spoke-wheel-like’ (defined as radiating enhancement from the center) enhancement of the patchy low-density area (n=1), which was associated with histologically identified intermediate disease stage; and astral low-density/hypointensity with marked enhancement (n=2) or a ‘rose flower’ sign (n=2), which was associated with histologically identified late disease stage. We hypothesized that the CT and MRI results of lymphadenitis in CSD may be associated with the pathological features. It may be suggested that the diagnosis of CSD may be formed when considering the characteristic CT and MRI features of astral low-density/hypointensity with marked enhancement or a ‘rose flower’ sign (defined as marginal petaloid enhancement) in the late disease stage, or the MRI results of homogeneous, moderate enhancement in the early disease stage, or the CT/MRI data of heterogeneous enhancement with non-enhancing area in the center in the intermediate disease stage, in solitary or multiple enlarged lymph nodes associated with general subcutaneous edema in the vicinity of the nodes on CT/MRI and with a history of cat exposure.
Cell-Intrinsic Expression of TLR9 in Autoreactive B Cells Constrains BCR/TLR7-Dependent Responses

Endosomal TLRs play an important role in systemic autoimmune diseases, such as systemic erythematosus lupus, in which DNA- and RNA-associated autoantigens activate autoreactive B cells through TLR9- and TLR7-dependent pathways. Nevertheless, TLR9-deficient autoimmune-prone mice develop more severe clinical disease, whereas TLR7-deficient and TLR7/9–double deficient autoimmune-prone mice develop less severe disease. To determine whether the regulatory activity of TLR9 is B cell intrinsic, we directly compared the functional properties of autoantigen-activated wild-type, TLR9-deficient, and TLR7-deficient B cells in an experimental system in which proliferation depends on BCR/TLR coengagement. In vitro, TLR9-deficient cells are less dependent on survival factors for a sustained proliferative response than are either wild-type or TLR7-deficient cells. The TLR9-deficient cells also preferentially differentiate toward the plasma cell lineage, as indicated by expression of CD138, sustained expression of IRF4, and other molecular markers of plasma cells. In vivo, autoantigen-activated TLR9-deficient cells give rise to greater numbers of autoantibody-producing cells. Our results identify distinct roles for TLR7 and TLR9 in the differentiation of autoreactive B cells that explain the capacity of TLR9 to limit, as well as TLR7 to promote, the clinical features of systemic erythematosus lupus.