Miracle-dz/UniFAR-FTUs huggingface dataset:
Dealloyed Intra-Nanogap Particles with Highly Robust, Quantifiable Surface-Enhanced Raman Scattering Signals for Biosensing and Bioimaging Applications

Uniformly controlling a large number of metal nanostructures with a plasmonically enhanced signal to generate quantitative optical signals and the widespread use of these structures for surface-enhanced Raman scattering (SERS)-based biosensing and bioimaging applications are of paramount importance but are extremely challenging. Here, we report a highly controllable, facile selective-interdiffusive dealloying chemistry for synthesizing the dealloyed intra-nanogap particles (DIPs) with a ∼2 nm intragap in a high yield (∼95%) without the need for an interlayer. The SERS signals from DIPs are highly quantitative and polarization-independent with polarized laser sources. Remarkably, all the analyzed particles displayed the SERS enhancement factors (EFs) of ≥1.1 × 108 with a very narrow distribution of EFs. Finally, we show that DIPs can be used as ultrasensitive SERS-based DNA detection probes for detecting 10 aM to 1 pM target concentrations and highly robust, quantitative real-time cell imaging probes for long-term imaging with low laser power and short exposure time.
Energy Cost of Common Physical Activities in Preschoolers.

BACKGROUND To determine the energy cost of common physical activities in preschoolers and to compare it with the Compendium of Energy Expenditure for Youth (CEEY). METHODS In total, 42 children [age: 4.8 (0.8) y; body mass index: 15.3 (2.0) kg/m2; 22 boys] completed 13 common physical activities covering sedentary to vigorous intensities, while energy expenditure (EE) was measured continuously by indirect calorimetry. Activity-specific metabolic equivalents (AME) were calculated as the EE observed during each single activity divided by the EE during observed rest. Independent t tests were applied to analyze differences between boys and girls and between AME and CEEY. RESULTS No significant differences in AME were observed between girls and boys. Except for playing hide-and-seek, all indoor activities revealed significantly higher energy costs compared with those stated in the compendium. Significant differences in outdoor activities were found for riding a tricycle [5.67 (95% confidence interval, 4.94-6.4) AME vs 6.2 metabolic equivalents, riding a bike, P < . 05] and for fast walking [5.42 (95% confidence interval, 4.84-6.0) AME vs 4.6 metabolic equivalents, P < . 05]. CONCLUSIONS Applying the CEEY to preschoolers will lead to a substantial underestimation of EE. Therefore, we recommend that a CEEY for preschool children be developed if measurement of EE is not feasible.
A CALL FOR FEATHER SAMPLING

1Department of Organismic Biology, Ecology, and Evolution, and Centerfor Tropical Research, Institute of the Environment, 621 Charles E. Young Drive South, Room LS5120, University of California, P.O. Box 951606, Los Angeles, California 90095-1606, USA; 2Smithsonian Environmental Research Center, P.O. Box 28, 647 Contees Wharf Road, Edgewater, Maryland 21037-0028, USA; 3School of Biological Sciences, Washington State University, P.O. Box 644236, Pullman, Washington 99164-4236, USA; Cornell Laboratory of Ornithology, 159 Sapsucker Woods Road, Ithaca, New York 14850, USA; 5Department of Evolution, Ecology, and Organismal Biology (EEOB), Ohio State University, 1735 Neil Avenue, Columbus, Ohio 43210-1293, USA; 6Department of Biological Sciences, Hanover, Dartmouth College, New Hampshire 03755, USA; 7Canadian Wildlife Service, 115 Perimeter Road, Saskatoon, Saskatchewan S7N 0X4, Canada; and 8Burke Museum, University of Washington, Box 353010, Seattle, Washington 98195-3010, USA
Development of a novel laser range scanner

Laser range scanning an organ surface intraoperatively provides a cost effective and accurate means of measuring geometric changes in tissue. A novel laser range scanner with integrated tracking was designed, developed, and analyzed with the goal of providing intraoperative surface data during neurosurgery. The scanner is fitted with passive spheres to be optically tracked in the operating room. The design notably includes a single-lens system capable of acquiring the geometric information (as a Cartesian point cloud) via laser illumination and charge-coupled device (CCD) collection, as well as the color information via visible light collection on the same CCD. The geometric accuracy was assessed by scanning a machined phantom of known dimensions and comparing relative distances of landmarks from the point cloud to the known distances. The ability of the scanner to be tracked was first evaluated by perturbing its orientation in front of the optical tracking camera and recording the number of spheres visible to the camera at each orientation, and then by observing the variance in point cloud locations of a fixed object when the tracking camera is moved around the scanner. The scanning accuracy test resulted in an RMS error of 0.47 mm with standard deviation of 0.40 mm. The sphere visibility test showed that four diodes were visible in most of the probable operating orientations, and the overall tracking standard deviation was observed to be 1.49 mm. Intraoperative collection of cortical surface scans using the new scanner is currently underway.
Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

Significance The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been used to generate targeted gene editing in plants. However, it is not known whether CRISPR/Cas-induced gene modifications in plants occur in somatic cells only or whether some or all of the modifications can enter the germ line to become heritable. Through systematic and multigenerational analysis, this study demonstrates that although the majority of gene modifications detected in the first generation CRISPR/Cas transgenic Arabidopsis plants were somatic mutations only, heritable mutations could be found in subsequent generations. In addition, deep sequencing of CRISPR/Cas-modified Arabidopsis genomes did not detect any off-targets. The work demonstrates that the CRISPR/Cas method can effectively create specific gene modifications in planta that are stably transmitted through the germ line to future generations. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has emerged as a powerful tool for targeted gene editing in many organisms, including plants. However, all of the reported studies in plants focused on either transient systems or the first generation after the CRISPR/Cas system was stably transformed into plants. In this study we examined several plant generations with seven genes at 12 different target sites to determine the patterns, efficiency, specificity, and heritability of CRISPR/Cas-induced gene mutations or corrections in Arabidopsis. The proportion of plants bearing any mutations (chimeric, heterozygous, biallelic, or homozygous) was 71.2% at T1, 58.3% at T2, and 79.4% at T3 generations. CRISPR/Cas-induced mutations were predominantly 1 bp insertion and short deletions. Gene modifications detected in T1 plants occurred mostly in somatic cells, and consequently there were no T1 plants that were homozygous for a gene modification event. In contrast, ∼22% of T2 plants were found to be homozygous for a modified gene. All homozygotes were stable to the next generation, without any new modifications at the target sites. There was no indication of any off-target mutations by examining the target sites and sequences highly homologous to the target sites and by in-depth whole-genome sequencing. Together our results show that the CRISPR/Cas system is a useful tool for generating versatile and heritable modifications specifically at target genes in plants.
Accuracy and Projection in Perceptions of Partners’ Recent Emotional Experiences: Both Minds Matter

In 2 studies involving 96 married couples (Study 1) and 118 romantic couples (Study 2), we investigated partners’ perceptions of each others’ recently experienced emotions. In both studies, both individuals within each couple independently provided reports of (a) their own recently experienced emotions, (b) their perceptions of their partners’ recently experienced emotions, and (c) the extent to which they had expressed the emotions they had experienced to their partner. We then assessed the extent to which perceptions of partners’ emotions were (a) accurate (i.e., in agreement with partners’ independent reports of their own feelings) and (b) a function of the perceiver’s own emotions (i.e., projected). Significant evidence for both accuracy in perceiving emotions (4 of 7 emotions in Study 1; 8 of 9 emotions in Study 2) and for projection of perceivers’ own emotions onto partners was obtained (5 of 7 emotions in Study 1; 9 of 9 emotions in Study 2). Effects for all remaining emotions trended in the same directions. There was almost no moderation of these effects by targets’ having knowingly expressed the emotions. Implications of the patterning of findings for different emotions for the social functions of accuracy and projection in perceiving emotions are discussed.
Welfare states, the Great Recession and health: Trends in educational inequalities in self-reported health in 26 European countries

Background Although socioeconomic inequalities in health have long been observed in Europe, few studies have analysed their recent patterning. In this paper, we examined how educational inequalities in self-reported health have evolved in different European countries and welfare state regimes over the last decade, which was troubled by the Great Recession. Methods We used cross-sectional data from the EU-SILC survey for adults from 26 European countries, from 2005 to 2014 (n = 3,030,595). We first calculated education-related absolute (SII) and relative (RII) inequalities in poor self-reported health by country-year, adjusting for age, sex, and EU-SILC survey weights. We then regressed the year- and country-specific RII and SII on a yearly time trend, globally and by welfare regime, adjusting for country fixed effects. We further adjusted the analysis for the economic cycle using GDP growth, unemployment, and income inequality. Results Overall, absolute inequalities persisted and relative inequalities slightly widened (betaRII = 0.0313, p<0.05). There were substantial differences by welfare regime: Anglo-Saxon countries experienced the largest increase in absolute inequalities (betaSII = 0.0032, p<0.05), followed by Bismarkian countries (betaSII = 0.0024, p<0.001), while they reduced in Post-Communist countries (betaSII = -0.0022, p<0.001). Post-Communist countries also experienced a widening in relative inequalities (betaRII = 0.1112, p<0.001), which were found to be stable elsewhere. Adjustment for income inequality only explained such trend in Anglo-Saxon countries. Conclusions Educational inequalities in health have overall persisted across European countries over the last decade. However, there is considerable variation across welfare regimes, possibly related to underpinning social safety nets and to austerity measures implemented during this 10-year period.
Affective Valence Signals Agency Within and Between Individuals

Affective valence is a core component of all emotional experiences. Building on recent evidence and theory, we reason that valence informs individuals about their agency—the mental capability of doing and intending. Expressed affect may also lead to perceptions of agency by others. Supporting the hypothesis that valence influences self- and other-perception of agency, across 5 studies, we showed that participants perceived more agency in themselves in positive versus neutral and negative personal (Study 1) and interpersonal (Study 2) events. Participants also perceived more agency in fictional characters showing positive versus negative affect, regardless of how acceptable the characters’ behavior was (Studies 3 and 4). Finally, we had participants personify 24 specific emotions across the valence dimension, and found that the more positive and less negative an emotion was, the more agency participants ascribed to the “person” (Study 5). We discuss the results in terms of how valence may help with human self- and social regulation.
A usable model of “decathlon winner” cancer cells in triple-negative breast cancer: survival of resistant cancer cells in quiescence

We previously described a strategy for selecting highly adaptable rare triple-negative breast cancer (TNBC) cells based on their ability to survive a severe and prolonged metabolic challenge, e.g., a lack of glutamine. We hypothesized that metabolically adaptable (MA) cancer cells selected from the SUM149 cell line in this manner have the capacity to survive a variety of challenges that postulated “decathlon winner” cancer cells must survive to succeed in metastasis. These MA cells were resistant to glutaminase inhibitor CB-839, as predicted from their ability to proliferate without exogenous glutamine. They were also resistant to hypoxia, surviving treatment with hypoxia inducer cobalt chloride. Investigating the nature of intrinsic resistance in SUM149-MA cells, we found that 1–2 mM metformin completely inhibited the emergence of MA colonies in SUM149 cells in glutamine-free medium. These highly resistant MA cells grew into colonies upon removal of metformin, indicating that they survived in quiescence for several weeks under metformin treatment. This approach of selecting resistant cells worked equally well with additional TNBC cell lines, specifically inflammatory breast cancer cell line FC-IBC02 and mouse breast cancer cell line 4T07. In both cases, less than 1% of cells survived metformin treatment and formed colonies in glutamine-free medium. The MA cells selected in this manner were significantly more resistant to the chemotherapeutic drug doxorubicin than the parental cell lines. We conclude that our approach may be useful in developing usable models of cancer cell quiescence and therapy resistance in TNBC.
Compact or spread? A quantitative spatial model of urban areas in Europe since 1990

Changes in urban residential density represent an important issue in terms of land consumption, the conservation of ecosystems, air quality and related human health problems, as well as the consequential challenges for urban and regional planning. It is the decline of residential densities, in particular, that has often been used as the very definition of sprawl, describing a phenomenon that has been extensively studied in the United States and in Western Europe. Whilst these studies provide valuable insights into urbanization processes, only a handful of them have reflected the uneven dynamics of simultaneous urban growth and shrinkage, using residential density changes as a key indicator to uncover the underlying dynamics. This paper introduces a contrasting analysis of recent developments in both de- and re-concentration, defined as decreasing or increasing residential densities, respectively. Using a large sample of European cities, it detects differences in density changes between successional population growth/decline. The paper shows that dedensification, found in some large cities globally, is not a universal phenomenon in growing urban areas; neither the increasing disproportion between a declining demand for and an increasing supply of residential areas nor actual concentration processes in cities were found. Thus, the paper provides a new, very detailed perspective on (de)densification in both shrinking and growing cities and how they specifically contribute to current land take in Europe.
The role of Octenidol®, Glandomed® and chlorhexidine mouthwash in the prevention of mucositis and in the reduction of the oropharyngeal flora: a double-blind randomized controlled trial

Aim: The oropharyngeal flora is of importance for the development of oral mucositis, which is a frequent complication in oncologic practice. It also plays a role in the pathogenesis of ventilator-associated pneumonia. Mucositis is associated with significantly worse clinical and economic outcomes. The aim of our study was to assess the efficacy of Octenidol®, Glandomed® and chlorhexidine mouthwash in the prevention of mucositis and reduction of the oropharyngeal flora. Methods: A prospective, double-blinded RCT including two strata was conducted between October 2008 and November 2010. Stratum i consisted of ventilated cardiothoracic surgical patients. Stratum ii consisted of medical patients with haemato-oncological malignancies requiring stem cell transplantation. The primary outcome measures were development of mucositis regarding to OMAS/WHO score and reduction of the oropharyngeal flora. Results: Both strata showed low OMAS/WHO scores which did not differ significantly between the groups. The overall mean reduction of colony forming units was significantly higher in the Octenidol® group compared to the chlorhexidine and the Glandomed® groups. Conclusions: No significant differences in the development of mucositis were found, thus all solutions proved successful in the prevention of mucositis. However, Octenidol® was superior in the reduction of the oropharyngeal flora. Hence, the preventive effect on nosocomial infections might be higher in patients using Octenidol® rather than chlorhexidine or Glandomed®.
Association between Physical Activity and Adiposity in Adolescents with Down Syndrome

Purpose Obesity is highly prevalent among adolescents with Down syndrome (DS); however, reported associations between body composition and moderate-to-vigorous physical activity (MVPA) have been small and nonsignificant. The purpose of this study was to compare group differences between adolescents with and without DS, including dual-energy x-ray absorptiometry (DXA) measured body composition and accelerometer-measured physical activity, and then examine associations within adolescents with DS. Methods Thirty-nine adolescents (22 with DS and 17 typically developing controls) 12–18 yr of age participated in the study. Groups had similar distributions of age, sex, and Tanner pubertal stage. Body composition was assessed by DXA, body mass index (BMI), and BMI percentile. MVPA was measured with ActiGraph GT3X+ accelerometers. Results Adolescents with DS had significantly higher BMI, BMI percentile, and DXA-derived percent body fat (%BF) as well as lower MVPA compared with controls (P < 0.05). Associations between MVPA and %BF in adolescents with DS were moderate (r = −0.39, P = 0.07) but substantially stronger than BMI (r = −0.19, P = 0.40). However, linear regression analyses identified Tanner stage (&bgr; = −0.77, P < 0.001) and MVPA (&bgr; = −0.34, P = 0.047) as significant predictors of %BF. No relevant associations between body composition and MVPA were observed in adolescents with typical development (P > 0.05). Conclusions Our findings suggest that MVPA is associated with adiposity when measured with DXA among adolescents with DS.
Serum Trace Elements and Heavy Metal Levels in Patients Diagnosed with Chronic Otitis Media and Their Association with Surgical Treatment Outcomes.

OBJECTIVE To determine the serum iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), magnesium (Mg), cobalt (Co), and lead (Pb) levels in patients with chronic otitis media (COM) and to evaluate the association of the serum levels of these elements with treatment outcomes. MATERIALS AND METHODS Thirty-one healthy volunteers and 31 patients with COM were prospectively included in this study. Serum levels of Fe, Zn, Mn, Mg, Cu, Co, and Pb were determined by an atomic absorption UNICAM-929 spectrophotometer. RESULTS Serum Co, Pb, and Fe levels were significantly increased (p<0.001) and serum Cu, Zn, Mg, and Mn levels were significantly reduced in patients with COM compared with controls (p<0.001). Serum Co and Mn levels were significantly decreased (p<0.001 and p<0.005, respectively) and serum Cu levels were significantly increased after surgery (p<0.005). The other evaluated blood chemicals and heavy metals did not exhibit significant differences (p>0.05). CONCLUSION Significant alterations in the serum chemical composition of patients with COM were observed. Moreover, with surgical treatment, serum levels of some of these chemicals were significantly altered. Further prospective studies are warranted to elucidate the exact association of these alterations in the etiopathogenesis of COM.
Using diagrams versus text for spaced restudy: Effects on learning in 10th grade biology classes.

BACKGROUND AND AIM Spaced restudy has been typically tested with written learning materials, but restudy with visual representations in actual classrooms is under-researched. We compared the effects of two spaced restudy interventions: A Diagram-Based Restudy (DBR) warm-up condition and a business-as-usual Text-Based Restudy (TBR) warm-up condition. SAMPLE One hundred and twenty-eight consented high school students in 15 classes. METHODS Students completed daily warm-ups over a 4-week period. Students were randomly assigned to conditions within classrooms. Warm-ups were independently completed at the start of class meetings and consisted of questions about content covered 1-10 days prior to each warm-up. Students received feedback on their answers each week. A series of ANOVAs and ANCOVAs was conducted. RESULTS AND CONCLUSIONS Results showed equal and significant growth from pre- to post-test for both conditions (d = .31-.67) on three outcomes: Biology knowledge, biology diagram comprehension (near transfer), and geology diagram comprehension (far transfer). ANCOVA results suggested that the magnitude of this increase was linked to the number of questions attempted during the intervention. For the DBR condition only, there were interactions with content knowledge on diagram comprehension gain scores and interactions with spatial scores on biology knowledge gain scores. Students with lower biology knowledge and lower Paper Folding Test scores were disadvantaged in the DBR condition, whereas the TBR condition was equitable across all levels of knowledge and spatial ability.