Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficient skyline computation in metric space

Given a set of n query points in a general metric space, a metric-space skyline (MSS) query asks what are the closest points to all these query points in the database. Here, consider for any point p, if there are no other points in the database which have less or equal distance to all the query points, then p is denoted as one of the closest points to the query points. This problem is a direct generalization of the recently proposed spatial-skyline query problem, where all the points are located in two or three dimensional Euclidean space. It is also closely related with the nearest neighbor (NN) query, the range query and the common skyline query problem. In this paper, we have developed new algorithms to aggressively prune non-skyline points from the search space. We also contribute two new optimization techniques to reduce the number of distance computations and dominance tests. Our experimental evaluation has shown the effectiveness and efficiency of our approach.
Immediate functional loading of implants in single tooth replacement: a prospective clinical multicenter study.

OBJECTIVES The aim of the present study was to evaluate the outcome of immediate functional loading of implants in single-tooth replacement using two different installation procedures. MATERIAL AND METHODS One hundred and fifty-one subjects, who required single-tooth rehabilitation in the area of 15-25 and 35-45, were enrolled in eight private clinics in Italy. The implant sites were randomly allocated to one of the following treatment groups. In the control group, in which a standard preparation procedure for implant placement and submerged healing of the implant was used, abutment connection and loading of the implants were performed 3 months after installation. In the test group 1, a standard preparation procedure for the implant placement and immediate functional loading of implant was carried out. In the test 2 group, however, a modified implant installation procedure (osteotome technique) was used followed by immediate functional loading of the implant. Clinical and radiographic examinations were performed at 3 and 12 months of follow-up at all sites. RESULTS Three implants (5.5%) from the test 2 group (osteotome preparation) and one (2%) from the test 1 group (conventional drill preparation) failed to integrate and were removed one and three months after implant installation. The mean marginal bone loss assessed at 12 months was 0.31 mm (test 1), 0.25 mm (test 2) and 0.38 mm (control) (no statistically significant differences were found between the three treatment groups.) CONCLUSION It is suggested that immediate functional loading of implants that are placed with a conventional installation technique and with sufficient primary stability may be considered as a valid treatment alternative in a single-tooth replacement.
Clinical Relevance of Loss of Atrial Sensing in Patients with Single Lead VDD Pacemakers

VAN CAMPEN, C.M. C. , et al.: Clinical Relevance of Loss of Atrial Sensing in Patients with Single Lead VDD Pacemakers. During single lead VDD pacing, loss of atrial sensing is reported to be 2%–11% of patients in the literature. The impact on quality‐of‐life and exercise duration has never been established. This study tried to assess the clinical relevance of loss of atrial sensing in patients with single lead VDD pacemakers. Twenty‐one patients with total AV block were studied 3 months after single lead VDD pacemaker implantation. During a 6‐minute walk test, atrial undersensing was verified and adjusted to achieve undersensing in < 5% (group 1), 5%–10% (group 2), and > 10% (group 3). Patients were allocated to each group in a randomized double blind crossover design. At the end of each 2‐month study period all patients underwent symptom limited treadmill exercise testing. Subjective assessment of exercise difficulty was made using the Borg score, and quality‐of‐life assessment was performed using the Health Status Questionnaire. Exercise duration significantly decreased in group 3 (228 ± 50 s) as compared to group 1 (257 ± 42 s) and group 2 (250 ± 46 sec) with an increase in the Borg score. Quality‐of‐life was decreased for three subscales in group 3 as compared to group 1 and group 2. In conclusion, atrial undersensing of > 10% in patients with single lead VDD pacing was associated with a decrease in exercise duration and increase in the subjective severity score, in addition to a decrease in quality‐of‐life. Atrial undersensing of < 10% did not effect exercise test results or quality‐of‐life. Since all studies reported intact atrioventricular synchrony in ≥ 90% of patients, loss of atrial sensing is of limited clinical importance in these patients.
Morphology of liquid microstructures on chemically patterned surfaces

We study the equilibrium conformations of liquid microstructures on flat but chemically heterogeneous substrates using energy minimization computations. The surface patterns, which establish regions of different surface energy, induce deformations of the liquid–solid contact line. Depending on the geometry, these deformations either promote or impede capillary breakup and bulge formation. The contact angles of the liquid on the hydrophilic and hydrophobic regions, as well as the pattern geometry and volume of liquid deposited, strongly affect the equilibrium shapes. Moreover, due to the small scale of the liquid features, the presence of chemical or topological surface defects significantly influence the final liquid shapes. Preliminary experiments with arrays of parallel hydrophilic strips produce shapes resembling the simulated forms. These encouraging results provide a basis for the development of high resolution lithography by direct wet printing.
Improving satisfaction with care and reducing length of stay in an obstetric triage unit using a nurse-midwife-managed model of care.

INTRODUCTION A quality improvement project was initiated at a tertiary-care center in a suburban area of the northeastern United States to determine whether length of stay and patient satisfaction in an obstetric triage unit could be improved by using a certified nurse-midwife (CNM) to manage and organize care in the triage unit. METHODS Patient satisfaction was measured using a previously validated instrument that consisted of 6 items using a 6-point Likert-type scale. The items measured patient satisfaction with: wait time for provider, information given, amount of time spent with provider, length of visit, overall care received, and overall triage experience. Patient satisfaction was measured before (n = 37) and after implementing CNM-managed care (n = 66) in an obstetrical triage unit. Length of stay in the triage unit was measured during standard care (n = 121) and after the implementation of CNM-managed care (n = 151) by recording the number of minutes women spent in the triage unit. RESULTS Participants in the CNM-managed care group reported increased patient satisfaction with care in 5 of the 6 aspects of satisfaction that were measured, including wait time for provider (P = .01), time spent with provider (P = .01), length of visit (P = .04), overall care received (P = .04), and overall triage experience (P = .01). The length of stay was significantly shorter for the women in the CNM-managed group (mean = 94.7 minutes; standard deviation [SD] 50.1) than for the women in the standard care model (mean = 122 minutes; SD = 66.8; P < .01). DISCUSSION The findings from this project suggest that a CNM-managed obstetric triage unit can improve satisfaction with care during the triage experience and reduce length of stay in the triage unit.
The Cost of Reducing the North Atlantic Ocean Biological Carbon Pump

To predict the impacts of climate change it is essential to understand how anthropogenic change alters the balance between atmosphere, ocean, and terrestrial reservoirs of carbon. It has been estimated that natural atmospheric concentrations of CO2 are almost 200 ppm lower than they would be without the transport of organic material produced in the surface ocean to depth, an ecosystem service driven by mechanisms collectively referred to as the biological carbon pump. Here we quantify potential reductions in carbon sequestration fluxes in the North Atlantic Ocean through the biological carbon pump over the twenty-first century, using two independent biogeochemical models, driven by low and high IPCC AR5 carbon emission scenarios. The carbon flux at 1000 m (the depth at which it is assumed that carbon is sequestered) in the North Atlantic was estimated to decline between 27 and 43% by the end of the century, depending on the biogeochemical model and the emission scenario considered. In monetary terms, the value of this loss in carbon sequestration service in the North Atlantic was estimated to range between US$170–US$3000 billion in abatement (mitigation) costs and US$23–US$401 billion in social (adaptation) costs, over the twenty-first century. Our results challenge the frequent assumption that coastal habitats store more significant amounts of carbon and are under greater threat. We highlight the largely unrecognized economic importance of the natural, blue carbon sequestration service provided by the open ocean, which is predicted to undergo significant anthropogenic-driven change.
Major Improvements to the Heliconius melpomene Genome Assembly Used to Confirm 10 Chromosome Fusion Events in 6 Million Years of Butterfly Evolution

The Heliconius butterflies are a widely studied adaptive radiation of 46 species spread across Central and South America, several of which are known to hybridize in the wild. Here, we present a substantially improved assembly of the Heliconius melpomene genome, developed using novel methods that should be applicable to improving other genome assemblies produced using short read sequencing. First, we whole-genome-sequenced a pedigree to produce a linkage map incorporating 99% of the genome. Second, we incorporated haplotype scaffolds extensively to produce a more complete haploid version of the draft genome. Third, we incorporated ∼20x coverage of Pacific Biosciences sequencing, and scaffolded the haploid genome using an assembly of this long-read sequence. These improvements result in a genome of 795 scaffolds, 275 Mb in length, with an N50 length of 2.1 Mb, an N50 number of 34, and with 99% of the genome placed, and 84% anchored on chromosomes. We use the new genome assembly to confirm that the Heliconius genome underwent 10 chromosome fusions since the split with its sister genus Eueides, over a period of about 6 million yr.
Three Cases of Carcinoid in the Equine Nasal Cavity and Maxillary Sinuses: Histologic and Immunohistochemical Features

Three cases of carcinoid tumor in horses are described. The tumors originated from the maxillary sinuses and the retrobulbar region and caused exophthalmos. Histologically, they had a characteristic endocrine pattern and were argyrophilic with the Grimelius stain. All tumors contained reactivity for neuron-specific enolase and synaptophysin. Two of three tumors were reactive for both bovine and porcine chromogranin A. These immunohistochemical results confirm the neuroendocrine nature of these tumors. Neuroendocrine cells could not be detected in the nasal mucosa and maxillary sinuses of a normal horse; therefore, the origin of these carcinoid tumors remains obscure.
Presemantic Cognition in Semantic Dementia: Six Deficits in Search of an Explanation

On the basis of a theory about the role of semantic knowledge in the recognition and production of familiar words and objects, we predicted that patients with semantic dementia would reveal a specific pattern of impairment on six different tasks typically considered pre- or non- semantic: reading aloud, writing to dictation, inflecting verbs, lexical decision, object decision, and delayed copy drawing. The prediction was that all tasks would reveal a frequency-by-typicality interaction, with patients performing especially poorly on lower-frequency items with atypical structure (e.g., words with an atypical spelling-to-sound relationship; objects with an atypical feature for their class, such as the hump on a camel, etc). Of 84 critical observations (14 patients performing 6 tasks), this prediction was correct in 84/84 cases; and a single component in a factor analysis accounted for 87 of the variance across seven measures: each patient's degree of impairment on atypical items in the six experimental tasks and a separate composite score reflecting his or her degree of semantic impairment. Errors also consistently conformed to the predicted pattern for both expressive and receptive tasks, with responses reflecting residual knowledge about the typical surface structure of each domain. We argue that these results cannot be explained as associated but unrelated deficits but instead are a principled consequence of a primary semantic impairment.
Effect of growth hormone deficiency on brain structure, motor function and cognition.

The growth hormone-insulin-like growth factor-1 axis plays a role in normal brain growth but little is known of the effect of growth hormone deficiency on brain structure. Children with isolated growth hormone deficiency (peak growth hormone <6.7 µg/l) and idiopathic short stature (peak growth hormone >10 µg/l) underwent cognitive assessment, diffusion tensor imaging and volumetric magnetic resonance imaging prior to commencing growth hormone treatment. Total brain, corpus callosal, hippocampal, thalamic and basal ganglia volumes were determined using Freesurfer. Fractional anisotropy (a marker of white matter structural integrity) images were aligned and tract-based spatial statistics performed. Fifteen children (mean 8.8 years of age) with isolated growth hormone deficiency [peak growth hormone <6.7 µg/l (mean 3.5 µg/l)] and 14 controls (mean 8.4 years of age) with idiopathic short stature [peak growth hormone >10 µg/l (mean 15 µg/l) and normal growth rate] were recruited. Compared with controls, children with isolated growth hormone deficiency had lower Full-Scale IQ (P < 0.01), Verbal Comprehension Index (P < 0.01), Processing Speed Index (P < 0.05) and Movement-Assessment Battery for Children (P < 0.008) scores. Verbal Comprehension Index scores correlated significantly with insulin-like growth factor-1 (P < 0.03) and insulin-like growth factor binding protein-3 (P < 0.02) standard deviation scores in isolated growth hormone deficiency. The splenium of the corpus callosum, left globus pallidum, thalamus and hippocampus (P < 0.01) were significantly smaller; and corticospinal tract (bilaterally; P < 0.045, P < 0.05) and corpus callosum (P < 0.05) fractional anisotropy were significantly lower in the isolated growth hormone deficiency group. Basal ganglia volumes and bilateral corticospinal tract fractional anisotropy correlated significantly with Movement-Assessment Battery for Children scores, and corpus callosum fractional anisotropy with Full-Scale IQ and Processing Speed Index. In patients with isolated growth hormone deficiency, white matter abnormalities in the corpus callosum and corticospinal tract, and reduced thalamic and globus pallidum volumes relate to deficits in cognitive function and motor performance. Follow-up studies that investigate the course of the structural and cognitive deficits on growth hormone treatment are now required to confirm that growth hormone deficiency impacts significantly on brain structure, cognitive function and motor performance.
A systematic review investigating interventions that can help reduce consumption of sugar-sweetened beverages in children leading to changes in body fatness

Background Both the prevalence of childhood obesity and the consumption of sugar-sweetened beverages (SSBs) have increased globally. The present review describes interventions that reduce the consumption of SSBs in children and determines whether this leads to subsequent changes in body fatness. Methods Three databases were searched from 2000 to August 2013. Only intervention control trials, ≥6 months in duration, which aimed to reduce the consumption of SSBs in >100 children aged 2–18 years, and reporting changes in body fatness, were included. The quality of selected papers was assessed. Results Eight studies met inclusion criteria. Six interventions achieved significant (P < 0.05) reductions in SSB intake, although this was not always sustained. In the two interventions providing replacement drinks, significant differences in body mass index (12- or 18-month follow-up) were reported (P = 0.001 and 0.045). The risk of being overweight/obesity was reduced (P < 0.05) in three of the five education programmes but in one programme only for girls who were overweight at baseline and in one programme only for pupils perceived to be at greater risk at baseline. In the one study that included both provision of water and education, the risk of being overweight was reduced by 31% (P = 0.04) in the intervention group. Conclusions The evidence suggests that school-based education programmes focusing on reducing SSB consumption, but including follow-up modules, offer opportunities for implementing effective, sustainable interventions. Peer support and changing the school environment (e.g. providing water or replacement drinks) to support educational programmes could improve their effectiveness. Home delivery of more suitable drinks has a big impact on reducing SSB consumption, with associated reductions in body weight.
The Plant-Specific ssDNA Binding Protein OSB1 Is Involved in the Stoichiometric Transmission of Mitochondrial DNA in Arabidopsis[W]

Plant mitochondrial genomes exist in a natural state of heteroplasmy, in which substoichiometric levels of alternative mitochondrial DNA (mtDNA) molecules coexist with the main genome. These subgenomes either replicate autonomously or are created by infrequent recombination events. We found that Arabidopsis thaliana OSB1 (for Organellar Single-stranded DNA Binding protein1) is required for correct stoichiometric mtDNA transmission. OSB1 is part of a family of plant-specific DNA binding proteins that are characterized by a novel motif that is required for single-stranded DNA binding. The OSB1 protein is targeted to mitochondria, and promoter–β-glucuronidase fusion showed that the gene is expressed in budding lateral roots, mature pollen, and the embryo sac of unfertilized ovules. OSB1 T-DNA insertion mutants accumulate mtDNA homologous recombination products and develop phenotypes of leaf variegation and distortion. The mtDNA rearrangements occur in two steps: first, homozygous mutants accumulate subgenomic levels of homologous recombination products; second, in subsequent generations, one of the recombination products becomes predominant. After the second step, the process is no longer reversible by backcrossing. Thus, OSB1 participates in controlling the stoichiometry of alternative mtDNA forms generated by recombination. This regulation could take place in gametophytic tissues to ensure the transmission of a functional mitochondrial genome.
Functional map of arrestin-1 at single amino acid resolution

Significance G protein-coupled receptors (GPCRs) comprise 2–3% of human proteins and are key for cellular communication in higher organisms. Thus, these receptors are essential in cell physiology, and their malfunction is commonly translated into pathological outcomes. Our structural knowledge of GPCRs has improved dramatically in recent years. The next big leap is deciphering the structure and function of their complexes with signaling proteins, and, specifically, the mechanisms by which these complexes form and activate. Our unbiased and complete scanning mutagenesis provides compelling functional insights into how a phosphorylated GPCR is desensitized by binding of arrestin. Combination of the discovered mutations will allow engineering of arrestins with either increased or reduced binding affinity with a broad range of applications in research, diagnostics, and pharmacology. Arrestins function as adapter proteins that mediate G protein-coupled receptor (GPCR) desensitization, internalization, and additional rounds of signaling. Here we have compared binding of the GPCR rhodopsin to 403 mutants of arrestin-1 covering its complete sequence. This comprehensive and unbiased mutagenesis approach provides a functional dimension to the crystal structures of inactive, preactivated p44 and phosphopeptide-bound arrestins and will guide our understanding of arrestin–GPCR complexes. The presented functional map quantitatively connects critical interactions in the polar core and along the C tail of arrestin. A series of amino acids (Phe375, Phe377, Phe380, and Arg382) anchor the C tail in a position that blocks binding of the receptor. Interaction of phosphates in the rhodopsin C terminus with Arg29 controls a C-tail exchange mechanism in which the C tail of arrestin is released and exposes several charged amino acids (Lys14, Lys15, Arg18, Lys20, Lys110, and Lys300) for binding of the phosphorylated receptor C terminus. In addition to this arrestin phosphosensor, our data reveal several patches of amino acids in the finger (Gln69 and Asp73–Met75) and the lariat loops (L249–S252 and Y254) that can act as direct binding interfaces. A stretch of amino acids at the edge of the C domain (Trp194–Ser199, Gly337–Gly340, Thr343, and Thr345) could act as membrane anchor, binding interface for a second rhodopsin, or rearrange closer to the central loops upon complex formation. We discuss these interfaces in the context of experimentally guided docking between the crystal structures of arrestin and light-activated rhodopsin.
DIAPH 3 governs the cellular transition to the amoeboid tumor phenotype

Thank you for the submission of your manuscript "DIAPH3 is a novel tumor suppressor near RB1 that governs the transition to the amoeboid phenotype" to EMBO Molecular Medicine. We have now heard back from the three referees whom we asked to evaluate your manuscript. You will see that they find the topic of your manuscript potentially interesting. However, they also raise significant concerns on the study, which should be addressed in a major revision of the manuscript.
Regulation of Recombinant Monoclonal Antibody Production in Chinese Hamster Ovary Cells: A Comparative Study of Gene Copy Number, mRNA Level, and Protein Expression

We performed a comparative analysis on CHO cells with varying recombinant monoclonal antibody production rates (qAb) and investigated the regulation of MAb production. Two families of CHO cells each composed of one parental and two progeny cell lines, generated by stepwise increases in methotrexate (MTX) concentration, were studied. The MAb heavy chain (HC) and light (LC) gene copy numbers and mRNA levels were quantitated by Southern and northern blotting in midexponential growth phase. We observed 2‐ to 3‐fold amplification in gene copy numbers in high producing cell lines when compared with parental cell lines. However, the mRNA levels in the high producers were 5‐ to 7‐fold higher, correlating well with the 5‐ to 7‐fold increase in qAb. These results were confirmed by real‐time qPCR analysis. Our study reveals that the MTX‐mediated MAb overexpression results from both an increase in the gene copy number and more efficient transcription of each gene copy. We also observed that the HC to LC gene copy ratio may affect the protein yield in MAb expression systems.