Miracle-dz/UniFAR-FTUs huggingface dataset:
Gene Expression Analysis of Xenopsylla cheopis (Siphonaptera: Pulicidae) Suggests a Role for Reactive Oxygen Species in Response to Yersinia pestis Infection

ABSTRACT Fleas are vectors for a number of pathogens including Yersinia pestis, yet factors that govern interactions between fleas and Y. pestis are not well understood. Examining gene expression changes in infected fleas could reveal pathways that affect Y. pestis survival in fleas and subsequent transmission. We used suppression subtractive hybridization to identify genes that are induced in Xenopsylla cheopis (Rothschild) (Siphonaptera: Pulicidae) in response to oral or hemocoel infection with Y. pestis. Overall, the transcriptional changes we detected were very limited. We identified several genes that are likely involved in the production or removal of reactive oxygen species (ROS). Midgut ROS levels were higher in infected fleas and antioxidant treatment before infection reduced ROS levels and resulted in higher bacterial loads. An ROS-sensitive mutant strain of Y. pestis lacking the OxyR transcriptional regulator showed reduced growth early after infection. Our results indicate that ROS may limit Y. pestis early colonization of fleas and that bacterial strategies to overcome ROS may enhance transmission.
SCAN as an investigative tool

Scientific content analysis (SCAN) is a technique that claims to enable the detection of deception in written statements. The underlying assumption is that statements of self-experienced events differ in several ways – such as liveliness and concreteness – from imaginary statements. It is used in many countries as an investigative tool. Nevertheless, little research on the reliability and validity of the SCAN technique is available. In this paper, two studies are presented. The first study focuses on the accuracy of SCAN to detect deception by three groups of raters with a different level of experience. This study shows a lack of validity of SCAN. Study 2 investigated the inter-rater reliability as a possible explanation for the poor validity results, and found little agreement between raters in identifying SCAN criteria. Overall, results indicate that the psychometric qualities of SCAN as an investigative tool is insufficient for use in police practice.
Fast Model-Based X-Ray CT Reconstruction Using Spatially Nonhomogeneous ICD Optimization

Recent applications of model-based iterative reconstruction (MBIR) algorithms to multislice helical CT reconstructions have shown that MBIR can greatly improve image quality by increasing resolution as well as reducing noise and some artifacts. However, high computational cost and long reconstruction times remain as a barrier to the use of MBIR in practical applications. Among the various iterative methods that have been studied for MBIR, iterative coordinate descent (ICD) has been found to have relatively low overall computational requirements due to its fast convergence. This paper presents a fast model-based iterative reconstruction algorithm using spatially nonhomogeneous ICD (NH-ICD) optimization. The NH-ICD algorithm speeds up convergence by focusing computation where it is most needed. The NH-ICD algorithm has a mechanism that adaptively selects voxels for update. First, a voxel selection criterion VSC determines the voxels in greatest need of update. Then a voxel selection algorithm VSA selects the order of successive voxel updates based upon the need for repeated updates of some locations, while retaining characteristics for global convergence. In order to speed up each voxel update, we also propose a fast 1-D optimization algorithm that uses a quadratic substitute function to upper bound the local 1-D objective function, so that a closed form solution can be obtained rather than using a computationally expensive line search algorithm. We examine the performance of the proposed algorithm using several clinical data sets of various anatomy. The experimental results show that the proposed method accelerates the reconstructions by roughly a factor of three on average for typical 3-D multislice geometries.
Chromatoid Bodies: Aggresome-like Characteristics and Degradation Sites for Organelles of Spermiogenic Cells

We investigated the localization of several markers for lysosomes and aggresomes in the chromatoid bodies (CBs) by immunoelectron microscopy. We found so-called aggresomal markers such as Hsp70 and ubiquitin in the core of the CBs and vimentin and proteasome subunit around the CBs. Ubiquitin-conjugating enzyme (E2) was also found in the CBs. In tubulovesicular structures surrounding the CBs, lysosomal markers were detected but an endoplasmic reticulum retention signal (KDEL) was not. Moreover, proteins located in each subcellular compartment, including the cytosol, mitochondria, and nucleus, were detected in the CBs. Signals for cytochrome oxidase I (COXI) coded on mitochondrial DNA were also found in the CBs. Quantitative analysis of labeling density showed that all proteins examined were concentrated in the CBs to some extent. These results show that the CBs have some aggresomal features, suggesting that they are not a synthetic site as proposed previously but a degradation site where unnecessary DNA, RNA, and proteins are digested.
Dynamic Diversity: Variety and Variation Within Countries

National models of social action over-privilege continuity and uniformity. They discount change — which they lack the capacity to explain (other than through exogenous shocks) — and neglect diversity within countries. This paper focuses on the national culture model which it argues requires commitment to illogical arguments and to suppositions which are theoretically and empirically untenable. An evaluation of each, it is argued, points to the existence of, and possibilities for, considerable national diversity and change — not pervasive and enduring national uniformity. Reflecting on the model’s rise and fall in anthropology, the paper also provides an outline explanation of its retention within organization studies and speculates about its future within that discipline.
Primary central nervous system T-cell lymphoma

Most primary CNS lymphomas (PCNSL) are B-cell neoplasms; T-cell lymphomas are quite rare. The authors report two young patients with T-cell PCNSL who had a complete response to chemo- and radiotherapy but developed recurrent disease and died 11 and 13 months from diagnosis. The prognosis of T-cell PCNSL may be worse than that of comparable B-cell tumors.
Clinical Study Serum Matrix Metalloproteinase-3 in Comparison with Acute Phase Proteins as a Marker of Disease Activity and Radiographic Damage in Early Rheumatoid Arthritis

1 Department of Internal Medicine, Faculty of Health Sciences, University of Pretoria, Private Bag X663, Pretoria 0001, South Africa 2 Division of Rheumatology, Department of Medicine, Chris Hani Baragwanath Hospital, University of the Witwatersrand, Johannesburg, South Africa 3Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria and Tshwane Academic Division of the National Health Laboratory Service, Pretoria, South Africa 4 Biostatistics and Epidemiology Division, School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Conference on ‘ Nutrition and age-related muscle loss , sarcopenia and cachexia ’ Symposium 4 : Sarcopenia and cachexia and social , clinical and public health dimensions The nursing contribution to nutritional care in cancer cachexia

Cancer cachexia is a complex syndrome. Its defining feature is involuntary weight loss, which arises, in part, because of muscle atrophy and is accompanied by functional decline. International expert consensus recommends that nutritional support and counselling is a component of multimodal therapy for cancer cachexia, as poor nutritional intake can contribute to progression of the syndrome. The present paper focuses on what is presently known about the nursing contribution to nutritional care in cancer cachexia. There is potential for nurses to play an important role. However, obstacles to this include lack of a robust evidence base to support their nutritional care practices and unmet need for education about nutrition in cancer. The nursing role’s boundaries and the outcomes of nurse-delivered nutritional care in cancer cachexia are both uncertain and should be investigated.
An Empirical Study of Operational Performance Convergence Following Enterprise-IT Implementation

This paper presents an empirical investigation into the influence of enterpriselevel software package implementation on operational performance. It seeks to determine whether, as some suggest, the implementation of these technologies leads to convergence in operational performance. The potential for performance convergence is important because if it occurs, then the use of packaged software applications may act as a leveler of competitive performance, rather than as a tool to promote competitive differentiation. This paper investigates operational performance change and convergence within three geographically defined operating regions of a single firm. Operational performance is measured using order lead-time – the elapsed time between when an order is received and when it is shipped to the customer. The firm implemented Enterprise Resource Planning (ERP) across its global operations, simultaneously in all regions, using single installation of the applications. Findings suggest that convergence may be an initial characteristic of initial operating performance following ERP deployment. However differences in rates of performance change following deployment led to divergence in operating performance. This suggests that convergence is not a long-run characteristic of post-ERP performance.
Cytoskeletal alterations in different developmental stages of in vivo cryopreserved preimplantation murine embryos

Background This study aimed to investigate the effects of vitrification and slow freezing on actin, tubulin, and nuclei of in vivo preimplantation murine embryos at various developmental stages using a Confocal Laser Scanning Microscope (CLSM). Material/Methods Fifty female mice, aged 4–6 weeks, were used in this study. Animals were superovulated, cohabitated overnight, and sacrificed. Fallopian tubes were excised and flushed. Embryos at the 2-cell stage were collected and cultured to obtain 4- and 8-cell stages before being cryopreserved using vitrification and slow freezing. Fixed embryos were stained with fluorescence-labelled antibodies against actin and tubulin, as well as DAPI for staining the nucleus. Labelled embryos were scanned using CLSM and images were analyzed with Q-Win software V3. Results The fluorescence intensity of both vitrified and slow-frozen embryos was significantly lower for tubulin, actin, and nucleus as compared to non-cryopreserved embryos (p<0.001). Intensities of tubulin, actin, and nucleus in each stage were also decreased in vitrified and slow-frozen groups as compared to non-cryopreserved embryos. Conclusions Cryopreservation of mouse embryos by slow freezing had a more detrimental effect on the actin, tubulin, and nucleus structure of the embryos compared to vitrification. Vitrification is therefore superior to slow freezing in terms of embryonic cryotolerance.
Mitochondrial phylogeography of the moor frog, Rana arvalis

The moor frog Rana arvalis is a lowland species with a broad Eurasiatic distribution, from arctic tundra through forest to the steppe zone. Its present‐day range suggests that glacial refugia of this frog were located outside southern European peninsulas. We studied the species‐wide phylogeographical pattern using sequence variation in a 682 base pairs fragment of mtDNA cytochrome b gene; 223 individuals from 73 localities were analysed. Two main clades, A and B, differing by c. 3.6% sequence divergence were detected. The A clade is further subdivided into two subclades, AI and AII differing by 1.0%. All three lineages are present in the Carpathian Basin (CB), whereas the rest of the species range, including huge expanses of Eurasian lowlands, are inhabited solely by the AI lineage. We infer that AII and B lineages survived several glacial cycles in the CB but did not expand, at least in the present interglacial, to the north. The geographical distribution and genealogical relationships between haplotypes from the AI lineage indicate that this group had two glacial refugia, one located in the eastern part of the CB and the other probably in southern Russia. Populations from both refugia contributed to the colonization of the western part of the range, whereas the eastern part was colonized from the eastern refugium only. The effective population size as evidenced by θML is an order of magnitude higher in the AI lineage than in the AII and B lineages. Demographic expansion was detected in all three lineages.
What examiners do: what thesis students should know

Although many articles have been written about thesis assessment, none provide a comprehensive, general picture of what examiners do as they assess a thesis. To synthesise this diverse literature, we reviewed 30 articles, triangulated their conclusions and identified 11 examiner practices. Thesis examiners tend to be broadly consistent in their practices and recommendations; they expect and want a thesis to pass, but first impressions are also very important. They read with academic expectations and the expectations of a normal reader. Like any reader, thesis examiners get annoyed and distracted by presentation errors, and they want to read a work that is a coherent whole. As academic readers, examiners favour a thesis with a convincing approach that engages with the literature and the findings, but they require a thesis to be publishable research. Finally, examiners give not only a final evaluation of a thesis, but also instruction and advice to improve the thesis and further publications and research. We hope that these generalisations will demystify the often secret process of assessing a thesis, and reassure, guide and encourage students as they write their theses.
Student Values, Religiosity, and Pro-social Behaviour: A Cross-national Perspective

The association between altruistic values, religious values and pro-social behaviour is well documented, though mainly in North America and across disparate demographic groups. However, we currently have no data that focus on the relationships between personal values, religious values and pro-social behaviour across many different countries. Our study provides this data. We surveyed the values and pro-social behaviour (giving donations and volunteer- ing) of university students in 14 different countries, thus achieving a unique cross-national perspective. We also included questions about materialistic values, which have hitherto been largely assumed to cause a reduction in pro-social behaviour. Our findings show that altruis- tic and religious values are positively significant in explaining variations in pro-social behav- iour, but that materialistic values are not negatively correlated with pro-social behaviour. Our study thus suggests that, in the modern world, materialistic, religious and altruistic values can combine in complex ways to determine pro-social behaviour and that this combination varies across countries and cultures. In the discussion section, we draw conclusions that also relate to religious institutions and their diaconia.
Does life stress moderate the effects of a physical activity intervention?

The study examined whether life stress moderated the effects of a physical activity intervention. Women (n = 184) and 154 men (61% non-Hispanic white, average age 24 years) from an urban university participated. Data were analyzed separately for men and women using a 2 × 2 ANCOVA adjusting for baseline physical activity (intervention vs. control × high life stress vs. low stress) with total physical activity at one-year and two-year assessments as dependent variables. No significant main effect for stress or condition was found. The interaction was significant (p = 0.015) for men at one-year, indicating that men in the intervention group with high stress were more active than low stress intervention men. Instruction received in the intervention group on the stress-relieving properties of physical activity may have helped men with high stress to adopt or maintain physical activity.
Linear-algebraic λ-calculus : higher-order , encodings and confluence .

We introduce a minimal language combining both higher-order computation and linear algebra. Roughly, this is nothing else than the λcalculus together with the possibility to make linear combinations of terms α. t + β. u. We describe how to “execute” this language in terms of a few rewrite rules, and justify them through the two fundamental requirements that the language be a language of linear operators, and that it be higherorder. We mention the perspectives of this work in field of quantum computation, which we show can be easily encoded in the calculus, as well as in other domains such as the interpretation of linear logic. Finally we prove the confluence of the calculus, this is our main result.
Relationship between hepatitis C genotype and severity of recurrent hepatitis C after liver transplantation.

BACKGROUND Recurrence of hepatitis C virus (HCV) infection after liver transplantation is universal, but the relationship between hepatitis C genotype and posttransplant outcome has been controversial. The aim of this study was to assess the relationship between hepatitis C genotype on posttransplant frequency of recurrent hepatitis, histologic severity of recurrence, and progression to cirrhosis. METHODS We studied 42 HCV RNA positive patients who received transplants between 1985 and 1994. Sera were tested for HCV RNA and protocol liver biopsies were in obtained the posttransplant period. Biopsies were scored according to the histologic activity index (HAI) and staged in a blinded fashion. RESULTS The distribution of hepatitis C genotypes distribution was as follows: 1a, 19 (45%); 1b, 17 (40%); 2b, 3 (7%); and 1 each of 2a, 3a, and 4a. There was histologic evidence of hepatitis in 38 of 42 (90.4%) of patients. Hepatitis C was mild, moderate, or severe (HAI>3) in 38% of grafts and minimal (HAI 0-3) in 62%. Overall HAI scores and histologic stage were higher in the genotype 1b group. Six of 17 (35%) genotype 1b patients had cirrhosis compared with 2 of 25 (8%) in the non-1b genotype group. CONCLUSIONS (1) Histologic evidence of recurrent hepatitis C is seen in 90% of liver allografts; (2) Histologic hepatitis C recurs with similar frequency in genotype 1b and non-1b recipients; (3) Genotype 1b is associated with more severe histologic disease recurrence than non-1b genotypes; (4) Genotype 1b appears to be associated with a higher degree of posttransplant fibrosis and cirrhosis than non-1b genotypes.
Correlates of HIV, sexually transmitted infections, and associated high-risk behaviors among male clients of female sex workers in Tijuana, Mexico

Objectives:To determine sociodemographic and behavioral correlates of HIV infection among male clients of female sex workers (FSWs) in Tijuana. Methods:Four hundred men aged 18 years or older who had paid or traded for sex with a FSW in Tijuana during the past 4 months were recruited in Tijuana's ‘zone of tolerance,’ where prostitution is practiced openly under a municipal permit system. Efforts were made to balance the sample between residents of the United States (San Diego County) and of Mexico (Tijuana). Participants underwent interviews and testing for HIV, syphilis, gonorrhea, and Chlamydia. Logistic regression identified correlates of HIV infection. Results:Mean age was 36.6 years. One-quarter had injected drugs within the previous 4 months. Lifetime use of heroin, cocaine, and methamphetamine was 36, 50, and 64%, respectively. Men had frequented FSWs for an average of 11 years, visiting FSWs an average of 26 times last year. In the past 4 months, one-half reported having unprotected sex with a FSW; 46% reported being high fairly or very often when having sex with a FSW. Prevalence of HIV, syphilis, gonorrhea, and Chlamydia was 4, 2, 2.5, and 7.5%; 14.2% were positive for at least one infection. Factors independently associated with HIV infection were living in Mexico, ever using methamphetamine, living alone, and testing positive for syphilis. Conclusion:Male clients of FSWs in Tijuana had a high sex and drug risk profile. Although sexually transmitted infection prevalence was lower than among FSWs, HIV prevalence was comparable suggesting the need for interventions among clients to prevent spread of HIV and sexually transmitted infections.
Amygdala and Dorsal Anterior Cingulate Connectivity during an Emotional Working Memory Task in Borderline Personality Disorder Patients with Interpersonal Trauma History

Working memory is critically involved in ignoring emotional distraction while maintaining goal-directed behavior. Antagonistic interactions between brain regions implicated in emotion processing, e.g., amygdala, and brain regions involved in cognitive control, e.g., dorsolateral and dorsomedial prefrontal cortex (dlPFC, dmPFC), may play an important role in coping with emotional distraction. We previously reported prolonged reaction times associated with amygdala hyperreactivity during emotional distraction in interpersonally traumatized borderline personality disorder (BPD) patients compared to healthy controls (HC): Participants performed a working memory task, while neutral versus negative distractors (interpersonal scenes from the International Affective Picture System) were presented. Here, we re-analyzed data from this study using psychophysiological interaction analysis. The bilateral amygdala and bilateral dorsal anterior cingulate cortex (dACC) were defined as seed regions of interest. Whole-brain regression analyses with reaction times and self-reported increase of dissociation were performed. During emotional distraction, reduced amygdala connectivity with clusters in the left dorsolateral and ventrolateral PFC was observed in the whole group. Compared to HC, BPD patients showed a stronger coupling of both seeds with a cluster in the right dmPFC and stronger positive amygdala connectivity with bilateral (para)hippocampus. Patients further demonstrated stronger positive dACC connectivity with left posterior cingulate, insula, and frontoparietal regions during emotional distraction. Reaction times positively predicted amygdala connectivity with right dmPFC and (para)hippocampus, while dissociation positively predicted amygdala connectivity with right ACC during emotional distraction in patients. Our findings suggest increased attention to task-irrelevant (emotional) social information during a working memory task in interpersonally traumatized patients with BPD.