Miracle-dz/UniFAR-FTUs huggingface dataset:
The contribution of beneath‐snow soil respiration to total ecosystem respiration in a high‐elevation, subalpine forest

The respiratory loss of CO2 from soil microbes beneath winter snow in forests from cold climates can significantly influence the annual carbon budget. We explored the magnitude of winter soil respiration using continuous measurements of beneath‐snow CO2 concentration within the footprint of a flux tower in a subalpine forest in the Rocky Mountains. We used eddy covariance measurements from the tower to obtain estimates of total wintertime ecosystem respiration and compared them to the calculated beneath‐snow CO2 flux. Soil respiration in the winter was estimated to contribute 35–48% of the total wintertime ecosystem respiration, and 7–10% of the total annual ecosystem respiration. The largest increase in soil respiration occurred in the late winter following an earlier‐than‐normal initiation of snowmelt and increase in snow density. Following this melt event, respiration rates increased approximately sixfold, despite an increase in soil temperature of only 0.3°–0.5°C. We interpret the late‐winter surge in soil respiration to be triggered by a strong response of beneath‐snow microbes to the pulse of meltwater coupled with extremely high sensitivity of the microbial biomass to increases in soil temperature.
Cerebralcare Granule® attenuates cognitive impairment in rats continuously overexpressing microRNA-30e

Previous studies have demonstrated that dysregulation of micro (mi)RNAs is associated with the etiology of various neuropsychiatric disorders, including depression and schizophrenia. Cerebralcare Granule® (CG) is a Chinese herbal medicine, which has been reported to have an ameliorative effect on brain injury by attenuating blood-brain barrier disruption and improving hippocampal neural function. The present study aimed to evaluate the cognitive behavior of rats continuously overexpressing miRNA-30e (lenti-miRNA-30e), prior to and following the administration of CG. In addition, the mechanisms underlying the ameliorative effects of CG were investigated. The cognitive ability of the rats was assessed using an open-field test and a Morris water maze spatial reference/working memory test. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay was used to detect neuronal apoptosis in the dentate gyrus of the hippocampus. Immunohistochemical analysis and western blotting were conducted to detect the expression levels of B-cell lymphoma 2 (BCL-2) and ubiquitin-conjugating enzyme 9 (UBC9), in order to examine neuronal apoptosis. The lenti-miRNA-30e rats exhibited increased signs of anxiety, depression, hyperactivity and schizophrenia, which resulted in a severe impairment in cognitive ability. Furthermore, in the dentate gyrus of these rats, the expression levels of BCL-2 and UBC9 were reduced and apoptosis was increased. The administration of CG alleviated cognitive impairment, enhanced the expression levels of BCL-2 and UBC9, and reduced apoptosis in the dentate gyrus in the lenti-miRNA-30e rats. No significant differences were detected in behavioral indicators between the lenti-miRNA-30e rats treated with CG and the normal controls. These findings suggested that CG exerts a potent therapeutic effect, conferred by its ability to enhance the expression levels of BCL-2 and UBC9, which inhibits the apoptotic process in neuronal cells. Therefore, CG may be considered a potential therapeutic strategy for the treatment of cognitive impairment in mental disorders.
Real Time Characterisation of the Mobile Multipath Channel

In this thesis a new approach for characterisation of digital mobile radio channels is investigated. The new approach is based on recognition of the fact that while the fading which is characteristic of the mobile radio channel is very rapid, the processes underlying this fading may vary much more slowly. The comparative stability of these underlying processes has not been exploited in system designs to date. Channel models are proposed which take account of the stability of the channel. Estimators for the parameters of the models are proposed, and their performance is analysed theoretically and by simulation and measurement. Bounds are derived for the extent to which the mobile channel can be predicted, and the critical factors which define these bounds are identified. Two main applications arise for these channel models. The first is the possibility of prediction of the overall system performance. This may be used to avoid channel fading (for instance by change of frequency), or compensate for it (by change of the signal rate or by power control). The second application is in channel equalisation. An equaliser based on a model which has parameters varying only very slowly can offer improved performance especially in the case of channels which appear to be varying so rapidly that the convergence rate of an equaliser based on the conventional model is not adequate. The first of these applications is explored, and a relationship is derived between the channel impulse response and the performance of a broadband system. Notation and Symbols . ∗ Complex conjugate ‖ · ‖ Frobenius norm of a matrix or 2-norm of a vector . + Moore-Penrose generalised inverse Element by element (Hadamard) product ~ Convolution ≡ Equivalence , Definition x · y Dot product between two spatial vectors x and y A Array steering matrix a Array steering vector b(·) Scattering function c Speed of light CN (·, ·) Complex normally distributed with given mean and covariance D, d Distances between source points and measurement points D Diagonal matrix of eigenvalues E{·} Expectation operator e. Exponential operator ei A vector of zeros with 1 in the i-th position f Discrete channel impulse response co-efficients g(·) Antenna gain pattern . H Complex Hermitian conjugate H (1) n (·) Order n Hankel function of the first kind h System or channel impulse response In(·) Order n Modified Bessel function of the first kind I Identity matrix Jn(·) Order n Bessel function of the first kind j √ −1 jn(·) Order n Spherical Bessel function J Fisher Information matrix K Row/Column reversing matrix k Wave number of signal carrier = 2π/λ
Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats

Granulocyte-colony stimulating factor (G-CSF) and bone marrow derived mononuclear cells (BM-MNCs) have both been shown to improve functional outcome following experimental stroke. These effects are associated with increased angiogenesis and neurogenesis. In the present study, we aimed to determine synergistic effects of G-CSF and BM-NMC treatment on long-term structural and functional recovery after photothrombotic stroke. To model the etiology of stroke more closely, we used spontaneously hypertensive (SH) rats in our experiment. Bone marrow derived mononuclear cells transplantation was initiated 1 h after the onset of photothrombotic stroke. Repeated G-CSF treatment commenced immediately after BM-MNC treatment followed by daily injections for five consecutive days. The primary endpoint was functional outcome after ischemia. Secondary endpoints included analysis of neurogenesis and angiogenesis as well as determination of infarct size. Granulocyte-colony stimulating factor treated rats, either in combination with BM-MNC or alone showed improved somatosensory but not gross motor function following ischemia. No beneficial effect of BM-MNC monotherapy was found. Infarct volumes were comparable in all groups. In contrast to previous studies, which used healthy animals, post-stroke neurogenesis and angiogenesis were not enhanced by G-CSF. In conclusion, the combination of G-CSF and BM-MNC was not more effective than G-CSF alone. The reduced efficacy of G-CSF treatment and the absence of any beneficial effect of BM-MNC transplantation might be attributed to hypertension-related morbidity.
The rs7044343 Polymorphism of the Interleukin 33 Gene Is Associated with Decreased Risk of Developing Premature Coronary Artery Disease and Central Obesity, and Could Be Involved in Regulating the Production of IL-33

Aim The effect of interleukin 33 (IL-33) in the inflammatory process generates significant interest in the potential significance of IL-33 as a biomarker for coronary artery disease (CAD). Here, our objective was to analyze whether IL-33 gene polymorphisms are associated with premature CAD in a case-control association study. Methods Four IL-33 polymorphisms (rs7848215, rs16924144, rs16924159 and rs7044343) were genotyped by 5’ exonuclease TaqMan assays in 1095 patients with premature CAD and 1118 controls. Results The rs7044343 T allele was significantly associated with a diminished risk of premature CAD (OR = 0.81, 95% CI: 0.69–0.97, Pdom = 0.020; OR = 0.85, 95% CI: 0.75–0.96, Padd = 0.019) and central obesity (OR = 0.74, 95% CI: 0.58–0.93, Pdom = 0.0007), respectively. When patients were divided into groups with and without type 2 diabetes mellitus (T2DM), the rs7044343 T allele was associated with a reduced risk of premature CAD in patients without (OR = 0.85, 95% CI: 0.73–0.99, Padd = 0.038) and with T2DM (OR = 0.61, 95% CI: 0.38–0.97, Pdom = 0.039; OR = 0.69, 95% CI: 0.49–0.97, Padd = 0.035). In order to establish the functional effect of the rs7044343 polymorphism, the production of IL-33 was determined in monocytes of selected individuals. Monocytes from individuals with rs7044343 CC genotype produced higher levels of IL-33 than monocytes from individuals with other genotypes. Conclusion The results suggest that the IL-33 rs7044343 T allele could be a susceptibility marker for premature CAD and central obesity. The rs7044343 polymorphism could be involved in regulating the production of IL-33.
Human locomotion on ice: the evolution of ice-skating energetics through history

SUMMARY More than 3000 years ago, peoples living in the cold North European regions started developing tools such as ice skates that allowed them to travel on frozen lakes. We show here which technical and technological changes determined the main steps in the evolution of ice-skating performance over its long history. An in-depth historical research helped identify the skates displaying significantly different features from previous models and that could consequently determine a better performance in terms of speed and energy demand. Five pairs of ice skates were tested, from the bone-skates, dated about 1800 BC, to modern ones. This paper provides evidence for the fact that the metabolic cost of locomotion on ice decreased dramatically through history, the metabolic cost of modern ice-skating being only 25% of that associated with the use of bone-skates. Moreover, for the same metabolic power, nowadays skaters can achieve speeds four times higher than their ancestors could. In the range of speeds considered, the cost of travelling on ice was speed independent for each skate model, as for running. This latter finding, combined with the accepted relationship between time of exhaustion and the sustainable fraction of metabolic power, gives the opportunity to estimate the maximum skating speed according to the distance travelled. Ice skates were probably the first human powered locomotion tools to take the maximum advantage from the biomechanical properties of the muscular system: even when travelling at relatively high speeds, the skating movement pattern required muscles to shorten slowly so that they could also develop a considerable amount of force.
Pharmacological Modulation of Hemodynamics in Adult Zebrafish In Vivo

Introduction Hemodynamic parameters in zebrafish receive increasing attention because of their important role in cardiovascular processes such as atherosclerosis, hematopoiesis, sprouting and intussusceptive angiogenesis. To study underlying mechanisms, the precise modulation of parameters like blood flow velocity or shear stress is centrally important. Questions related to blood flow have been addressed in the past in either embryonic or ex vivo-zebrafish models but little information is available for adult animals. Here we describe a pharmacological approach to modulate cardiac and hemodynamic parameters in adult zebrafish in vivo. Materials and Methods Adult zebrafish were paralyzed and orally perfused with salt water. The drugs isoprenaline and sodium nitroprusside were directly applied with the perfusate, thus closely resembling the preferred method for drug delivery in zebrafish, namely within the water. Drug effects on the heart and on blood flow in the submental vein were studied using electrocardiograms, in vivo-microscopy and mathematical flow simulations. Results Under control conditions, heart rate, blood flow velocity and shear stress varied less than ± 5%. Maximal chronotropic effects of isoprenaline were achieved at a concentration of 50 μmol/L, where it increased the heart rate by 22.6 ± 1.3% (n = 4; p < 0.0001). Blood flow velocity and shear stress in the submental vein were not significantly increased. Sodium nitroprusside at 1 mmol/L did not alter the heart rate but increased blood flow velocity by 110.46 ± 19.64% (p = 0.01) and shear stress by 117.96 ± 23.65% (n = 9; p = 0.03). Discussion In this study, we demonstrate that cardiac and hemodynamic parameters in adult zebrafish can be efficiently modulated by isoprenaline and sodium nitroprusside. Together with the suitability of the zebrafish for in vivo-microscopy and genetic modifications, the methodology described permits studying biological processes that are dependent on hemodynamic alterations.
HLA Preferences for Conserved Epitopes: A Potential Mechanism for Hepatitis C Clearance

Hepatitis C virus (HCV) infections affect more than 170 million people worldwide. Most of these individuals are chronically infected, but some clear the infection rapidly. Host factors seem to play a key role in HCV clearance, among them are the human leukocyte antigen (HLA) class I molecules. Certain HLA molecules, e.g., B*27 and B*57, are associated with viral clearance. To identify potential mechanisms for these associations, we assess epitope distribution differences between HLA molecules using experimentally verified and in silico predicted HCV epitopes. Specifically, we show that the NS5B protein harbors the largest fraction of conserved regions among all HCV proteins. Such conserved regions could be good targets for cytotoxic T-cell (CTL) responses. We find that the protective HLA-B*27 molecule preferentially presents cytotoxic T-cell (CTL) epitopes from NS5B and, in general, presents the most strongly conserved epitopes among the 23 HLA molecules analyzed. In contrast, HLA molecules known to be associated with HCV persistence do not have similar preferences and appear to target the variable P7 protein. Overall, our analysis suggests that by targeting highly constrained – and thereby conserved – regions of HCV, the protective HLA molecule HLA-B*27 reduces the ability of HCV to escape the cytotoxic T-cell response of the host. For visualizing the distribution of both experimentally verified and predicted epitopes across the HCV genome, we created the HCV epitope browser, which is available at theory.bio. uu.nl/ucqi/hcv.
M2M meets D2D: Harnessing D2D interfaces for the aggregation of M2M data

Direct device-to-device (D2D) communication presents as an effective technique to reduce the load at the base station (BS) while ensuring reliable localized communication. In this paper, we propose a large-scale M2M data Aggregation and Trunking (MAT) scheme, whereby the user equipments (UEs) aggregate M2M data from the nearby MTDs and trunk this data along with their own data to the BS in the cellular uplink. We develop a comprehensive stochastic geometry framework by considering a Poisson hard sphere model for UE coverage. The main motivation of this model is to capture the fact that a UE can gather data from short range, low-power MTDs located only in its close proximity while ensuring that an MTD is associated to at most one UE. We explore the inherent trade-off between the time reserved for aggregation and successful trunking of data to the BS and compare our results with the baseline case where no aggregation mechanism is used. We show that while the baseline case of connecting a bulk of MTDs directly with the BS is prohibitive, MAT scheme can efficiently gather data from selected MTDs in a distributed manner.
On the Constant Diagnosability of Baseline Interconnection Networks

A novel approach for the diagnosis of baseline interconnection networks with a fan-in/fan-out of 2 is presented. The totally exhaustive combinatorial fault model with single fault assumption is used in the analysis. Some new characteristics of baseline interconnection networks are proved. A characterization for the fault location and the fault type of the one-response fault are given. This characterization is used in proving that baseline interconnection networks with fan-in/fan-out of 2 can be diagnosed with a constant number of tests independent of the network size. The maximum number of tests is 12. >
Effective Target Size for the Induction of Bystander Effects in Medium Transfer Experiments

Abstract Liu, Z. , Prestwich, W. V. , Stewart, R. D. , Byun, S. H. , Mothersill, C. E. , McNeill, F. E. and Seymour, C. B. Effective Target Size for the Induction of Bystander Effects in Medium Transfer Experiments. Radiat. Res. 168, 627–630 (2007). Although radiation-induced bystander effects are frequently observed biological phenomena, the mechanism for these effects has not been fully determined. The target-hit theory and related concepts from microdosimetry provide a convenient formalism to help identify the nature of the targets responsible for initiating the emission of diffusible factors in medium transfer experiments. We used the microdosimetric models proposed by Stewart et al. (Radiat. Res. 165, 460–469, 2006) to analyze the results of published medium transfer experiments for γ-ray doses in the range of 0.04 mGy to 5 Gy. The analysis suggests that the effective size of the target responsible for initiating signal emission in HPV-G human keratinocyte donor cells is approximately 2 μm.
Simulation-based computation of information rates: upper and lower bounds

It has recently become feasible to compute information rates of finite-state source/channel models with memory. Such methods can also be used to compute upper and lower bounds on the information rate of very general (nonfinite-state) channels with memory by means of finite-state approximations. Further numerical bounds are obtained from reduced-state computations.
High Uptake of Exclusive Breastfeeding and Reduced Early Post-Natal HIV Transmission

Background Empirical data showing the clear benefits of exclusive breastfeeding (EBF) for HIV prevention are needed to encourage implementation of lactation support programs for HIV-infected women in low resource settings among whom replacement feeding is unsafe. We conducted a prospective, observational study in Lusaka, Zambia, to test the hypothesis that EBF is associated with a lower risk of postnatal HIV transmission than non-EBF. Methods and Results As part of a randomized trial of early weaning, 958 HIV-infected women and their infants were recruited and all were encouraged to breastfeed exclusively to 4 months. Single-dose nevirapine was provided to prevent transmission. Regular samples were collected from infants to 24 months of age and tested by PCR. Detailed measurements of actual feeding behaviors were collected to examine, in an observational analysis, associations between feeding practices and postnatal HIV transmission. Uptake of EBF was high with 84% of women reporting only EBF cumulatively to 4 months. Post-natal HIV transmission before 4 months was significantly lower (p = 0.004) among EBF (0.040 95% CI: 0.024–0.055) than non-EBF infants (0.102 95% CI: 0.047–0.157); time-dependent Relative Hazard (RH) of transmission due to non-EBF = 3.48 (95% CI: 1.71–7.08). There were no significant differences in the severity of disease between EBF and non-EBF mothers and the association remained significant (RH = 2.68 95% CI: 1.28–5.62) after adjusting for maternal CD4 count, plasma viral load, syphilis screening results and low birth weight. Conclusions Non-EBF more than doubles the risk of early postnatal HIV transmission. Programs to support EBF should be expanded universally in low resource settings. EBF is an affordable, feasible, acceptable, safe and sustainable practice that also reduces HIV transmission providing HIV-infected women with a means to protect their children's lives. Trial Registration ClinicalTrials.gov NCT00310726
Non-destructive diagnostic facilities for cable systems

Polymeric materials like polyethylene and silicone rubber used in cables, joints and terminations show an electrical aging behavior. Considering the consequences of dielectric aging, effective diagnostic facilities are currently required, which have to be primarily nondestructive and which should detect as many faults as possible. Based on previous experiences it can be expected, that ultrasonic measurements and nuclear magnetic resonance (NMR) investigations fulfill these requirements. In addition the possible use of these facilities for quality management is presently being investigated. The increasing miniaturization, combined with the possible application of mobile diagnostic devices, is taken into account. First results show the feasible use of these facilities for detecting inhomogeneities like impurities and electrical trees in the insulation material and for identifying variations of the cross-link density.
Use and Avoidance of Seclusion and Restraint: Consensus Statement of the American Association for Emergency Psychiatry Project BETA Seclusion and Restraint Workgroup

Issues surrounding reduction and/or elimination of episodes of seclusion and restraint for patients with behavioral problems in crisis clinics, emergency departments, inpatient psychiatric units, and specialized psychiatric emergency services continue to be an area of concern and debate among mental health clinicians. An important underlying principle of Project BETA (Best practices in Evaluation and Treatment of Agitation) is noncoercive de-escalation as the intervention of choice in the management of acute agitation and threatening behavior. In this article, the authors discuss several aspects of seclusion and restraint, including review of the Centers for Medicare and Medicaid Services guidelines regulating their use in medical behavioral settings, negative consequences of this intervention to patients and staff, and a review of quality improvement and risk management strategies that have been effective in decreasing their use in various treatment settings. An algorithm designed to help the clinician determine when seclusion or restraint is most appropriate is introduced. The authors conclude that the specialized psychiatric emergency services and emergency departments, because of their treatment primarily of acute patients, may not be able to entirely eliminate the use of seclusion and restraint events, but these programs can adopt strategies to reduce the utilization rate of these interventions.