Miracle-dz/UniFAR-FTUs huggingface dataset:
Interface engineering to control charge transport in colloidal semiconductor nanowires and nanocrystals

Colloidal semiconductor nanocrystals (NCs) are a class of materials that has rapidly gained prominence and has shown the potential for large area electronics. These materials can be synthesized cheaply and easily made in high quality, with tunable electronic properties. However, evaluating if colloidal nanostructures can be used as a viable semiconducting material for large area electronics and more complex integrated circuits has been a long standing question in the field. When these materials are integrated into solid-state electronics, multiple interfaces need to be carefully considered to control charge transport, these interfaces are the: metal contact/ semiconductor, dielectric/semiconductor and the nanocrystal surface. Here, we use colloidal nanowire (NW) field-effect transistors (FETs) as a model system to understand doping and hysteresis. Through controllable doping, we fabricated PbSe NW inverters that exhibit amplification and demonstrate that these nanostructured materials could be used in more complex integrated circuits. By manipulating the dielectric interface, we are able to reduce the hysteresis and make low-voltage, low-hysteresis PbSe NW FETs on flexible plastic, showing the promise of colloidal nanostructures in large area flexible electronics. In collaboration, we are able to fabricate high-performance CdSe NC FETs through the use of a novel ligand, ammonium thiocyanate to enhance electronic coupling, and extrinsic atom in indium to dope and passivate surface traps, to yield mobilities exceeding 15 cm2V-1s-1. Combining high-mobility CdSe NC FETs with our low-voltage plastic platform, we were able to translate the exceptional devices performances on flexible substrates. This enables us to construct, for the first time, nanocrystal integrated circuits (NCICs) constructed from multiple well-behaved, high-performance NC-FETs. These transistors operate with small variations in device parameters over large area in concert, enabling us to fabricate NCIC inverters, amplifiers and ring oscillators. Device performance is comparable to other emerging solution-processable materials, demonstrating that this class of colloidal NCs as a viable semiconducting material for large area electronic applications. Degree Type Dissertation Degree Name Doctor of Philosophy (PhD) Graduate Group Materials Science & Engineering First Advisor Cherie R. Kagan
Dissent in Numbers: Making Strong Anonymity Scale

Current anonymous communication systems make a trade-off between weak anonymity among many nodes, via onion routing, and strong anonymity among few nodes, via DC-nets. We develop novel techniques in Dissent, a practical group anonymity system, to increase by over two orders of magnitude the scalability of strong, traffic analysis resistant approaches. Dissent derives its scalability from a client/server architecture, in which many unreliable clients depend on a smaller and more robust, but administratively decentralized, set of servers. Clients trust only that at least one server in the set is honest, but need not know or choose which server to trust. Unlike the quadratic costs of prior peer-to-peer DC-nets schemes, Dissent's client/server design makes communication and processing costs linear in the number of clients, and hence in anonymity set size. Further, Dissent's servers can unilaterally ensure progress, even if clients respond slowly or disconnect at arbitrary times, ensuring robustness against client churn, tail latencies, and DoS attacks. On DeterLab, Dissent scales to 5,000 online participants with latencies as low as 600 milliseconds for 600-client groups. An anonymous Web browsing application also shows that Dissent's performance suffices for interactive communication within smaller local-area groups.
The sociability of computer-supported collaborative learning environment

There is much positive research on computer -supported collaborative learning (CSCL) environments in asynchronous distributed learning groups (DLGs). There is also research that shows that contemporary CSCL environments do not completely fulfil expectations on supporting interactive group learning, shared understanding, social construction of knowledge, and acquisition of competencies. There appear to be two major pitfalls impeding achievement of the desired social interaction in CSCL environments: taking social interaction in groups for granted and the lack of attention paid to the social psychological dimension of social interaction outside of the task context. Current solutions offered to avoid the pitfalls placed responsibility on instructors and teachers to encourage collaborative learning and social interaction. To both free educators from this burden and be more cost effective, we propose an intelligent CSCL environment. The environment is based upon a theoretical framework that suggests embedding certain properties in the environment to act as social contextual facilitators - social affordances - to initiate and sustain learner’s social interactions. Finally, a group awareness widget (GAW) - a software tool providing the learner group awareness about the others in the task and in the non-task context - is introduced as an embodiment of this theoretical framework.
Next generation data integration for Life Sciences

Ever since the advent of high-throughput biology (e.g., the Human Genome Project), integrating the large number of diverse biological data sets has been considered as one of the most important tasks for advancement in the biological sciences. Whereas the early days of research in this area were dominated by virtual integration systems (such as multi-/federated databases), the current predominantly used architecture uses materialization. Systems are built using ad-hoc techniques and a large amount of scripting. However, recent years have seen a shift in the understanding of what a “data integration system” actually should do, revitalizing research in this direction. In this tutorial, we review the past and current state of data integration for the Life Sciences and discuss recent trends in detail, which all pose challenges for the database community.
Preimplantation genetic diagnosis for a couple with recurrent pregnancy loss and triploidy.

BACKGROUND Triploidy may arise from fertilization of a mature haploid egg by two haploid sperm or by failure of meiotic divisions yielding a diploid gamete. We encountered a couple with habitual abortion, in which the last two fetuses were documented as viable triploid. METHODS To avoid dispermic penetration and development of abnormal preembryos, insemination was done by intracytoplasmic sperm injection (ICSI) followed by fluorescence in situ hybridization (FISH) of biopsied blastomeres. RESULTS Tests of the husband's spermatozoa by FISH, revealed that only 2-3% of the sperm were disomic for chromosomes 16, 13, 21, X, and Y. No triple disomy was detected among chromosomes 16, 13 and 21, which makes it very unlikely that triploidy resulted from diploid spermatozoa. Following a controlled ovulation induction protocol, low quality oocytes with immature cumuli were revealed. After ICSI, five eggs became two pronuclei (2PN) zygotes and none of the other eggs developed a 3PN zygote. FISH was performed on chromosomes 16 and 21 in four preembryos developed to a 6-8 cell stage. Aneuploidy or mosaicism for each of these chromosomes was detected in one preembryo and later in two disaggregated blastocysts. FISH failed in one preembryo that became atretic after biopsy. CONCLUSIONS Although this case was unsuccessful in achieving embryo transfer and normal pregnancy, we detected many abnormal morphological features in the oocytes and chromosomal abnormalities in the cleaving preembryos. This protocol can be proposed to patients with recurrent pregnancy loss associated with chromosomal abnormalities in the fetus.
Identification of Active-Site Amino Acid Residues in the Chiba Virus 3C-Like Protease

ABSTRACT The 3C-like protease of the Chiba virus, a Norwalk-like virus, is one of the chymotrypsin-like proteases. To identify active-site amino acid residues in this protease, 37 charged amino acid residues and a putative nucleophile, Cys139, within the GDCG sequence were individually replaced with Ala in the 3BC precursor, followed by expression in Escherichia coli, where the active 3C-like protease would cleave 3BC into 3B (VPg) and 3C (protease). Among 38 Ala mutants, 7 mutants (R8A, H30A, K88A, R89A, D138A, C139A, and H157A) completely or nearly completely lost the proteolytic activity. Cys139 was replaceable only with Ser, suggesting that an SH or OH group in the less bulky side chain was required for the side chain of the residue at position 139. His30, Arg89, and Asp138 could not be replaced with any other amino acids. Although Arg8 was also not replaceable for the 3B/3C cleavage and the 3C/3D cleavage, the N-terminal truncated mutant devoid of Arg8 significantly cleaved 3CD into 3C and 3D (polymerase), indicating that Arg8 itself was not directly involved in the proteolytic cleavage. As for position 88, a positively charged residue was required because the Arg mutant showed significant activity. As deduced by the X-ray structure of the hepatitis A virus 3C protease, Arg8, Lys88, and Arg89 are far away from the active site, and the side chain of Asp138 is directed away from the active site. Therefore, these are not catalytic residues. On the other hand, all of the mutants of His157 in the S1 specificity pocket tended to retain very slight activity, suggesting a decreased level of substrate recognition. These results, together with a sequence alignment with the picornavirus 3C proteases, indicate that His30 and Cys139 are active-site residues, forming a catalytic dyad without a carboxylate directly participating in the proteolysis.
Immunohistochemical characterization of the cutaneous cellular infiltrate in different areas of chronic leg ulcers

Current understanding of the immunological mechanisms involved in the pathogenesis of venous leg ulcers is insufficient. In this study the cellular composition of skin biopsies taken from the center, the edge, and 2 cm distant from the edge of venous leg ulcers was characterized quantitatively by immunohistochemical staining. In the epidermis the mean numbers of Langerhans cells (CD1a+) were four times lower at the edge of the ulcer compared to clinically intact epidermis 2 cm distant from the edge. In the dermis a statistically significant increase in the mean numbers of macrophages (CD68+) and neutrophils (NP57+) from the distant area towards the center of the ulcer was observed. No significant differences were observed in the distribution of T cells nor in the ratio of CD4+/CD8+ T‐cell subsets between the different regions of the ulcer. About 30% of T lymphocytes were CD8+ in all microenvironments. The center and the edge of the ulcer were dominated by macrophages comprising 63% and 53% of the cells respectively, while T lymphocytes dominated the distant area. The area 2 cm distant from the edge was also heavily infiltrated by macrophages and neutrophils. B cells (CD22+) and NK cells (CD56+) were relatively rare in all areas, comprising less than 3% of the dermal infiltrate. In conclusion, local microenvironments each with a different cellular composition can be defined within venous leg ulcers.
Silver-Stained Nucleolar Organizer Regions in Normal and Dysplastic Cervical Lesions: Correlation with DNA Ploidy and S-Phase Fraction by Flow Cytometry

Objectives: To investigate the prognostic significance of DNA ploidy and silver-stained nucleolar organizer regions (AgNOR), their relation to the cytological grades of cervical dysplasia and correlation of cells in the S-phase of the cell cycle and DNA ploidy with AgNOR count. Methods: Multiparametric DNA flow-cytometric analysis and AgNOR count were performed on cervical smear samples, cytologically diagnosed as normal (n = 196), atypical squamous cells of unknown significance (ASCUS, n = 98) and various grades of cervical dysplasia (n = 127). Results: Among the cytological grade, aneuploid cases from low-grade squamous intraepithelial lesion (mild) and high-grade squamous intraepithelial lesion (moderate and severe) groups were showing high AgNOR counts (ranges 4.33–5.86 and 5.91–7.42, respectively). Aneuploid cases from the ASCUS group also presented high AgNOR (range 3.72–4.93). The high percentage of cells in the S-phase (>12%) correlated with these 2 parameters in the ASCUS and dysplasia groups. After grouping the cases into those with high (>4.0) and low (<4.0) AgNOR counts, significant correlations in the group with high AgNOR count with DNA ploidy, S-phase and cytological grade were recorded (p < 0.001). Results obtained from follow-up study revealed that cases with aneuploidy, high AgNOR count and S-phase fraction presented with a progressive trend of disease. Conclusions: Thus, the study shows that high AgNOR count, S-phase fraction and aneuploid DNA have prognostic significance in the early detection of cervical cancer.
Inhibitory effects of pyrrolidine dithiocarbamate on endotoxin-induced uveitis in Lewis rats.

PURPOSE To determine the effect of pyrrolidine dithiocarbamate (PDTC), an antioxidant nuclear factor (NF)-kappaB inhibitor, on the ocular inflammation induced by lipopolysaccharide (LPS). METHODS Endotoxin-induced uveitis (EIU) was produced by a footpad injection of 200 microg LPS in male Lewis rats. PDTC (200 mg/kg) was injected intraperitoneally 30 minutes before the LPS administration. The number of infiltrating cells and protein concentration in the aqueous humor (AqH) was determined from the AqH collected at 24 hours. Immunohistochemical staining with a monoclonal antibody against activated NF-kappaB was performed to evaluate the effect of PDTC on NF-kappaB activation. Interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha mRNA expression in the iris-ciliary body (ICB) was determined by RNase protection assay (RPA). The levels of these cytokines and nitric oxide (NO) production were also determined. RESULTS The number of cells in the AqH was 1100 +/- 254 cells/microL in rats injected with LPS and 90 +/- 43 cells/microL in rats pretreated with PDTC (P < 0.001). The concentration of proteins was significantly lower in the AqH of rats pretreated with PDTC than in those without PDTC. The number of activated NF-kappaB-positive cells in the ICB was reduced by the PDTC treatment. The ICB at 6 hours after LPS injection exhibited increased expression of IL-1beta, IL-6, and TNF-alpha mRNAs, which was decreased after PDTC pretreatment. PDTC also significantly diminished the levels of these cytokines and nitrite-nitrate in the AqH. CONCLUSIONS These results suggest that PDTC reduces ocular inflammation in eyes with EIU by downregulating proinflammatory cytokine expression and by inhibiting the NF-kappaB-dependent signaling pathway.
Sudoriferous Cyst of the Orbit

Purpose A case of sudoriferous cyst of the orbit in an adult patient is reported. This unusual cyst in the orbit has heretofore been reported only in congenital cases. Methods Case report. Results A 45-year-old woman sought treatment for an asymptomatic mass of the left lower eyelid. Computed tomography of the orbits showed a cystic lesion in the inferior orbit with bony remodeling. Histopathologic examination showed the lesion to be a solitary cyst lined by two to three layers of cuboidal epithelial cells with eosinophilic cytoplasm. Some of the epithelial cells contained periodic acid–Schiff-positive, diastase-resistant granules. The pathologic diagnosis was a sudoriferous cyst. Conclusion Sudoriferous cysts, although uncommon, should be included in the differential diagnosis of orbital cystic tumors.
Performance of Some Ridge Parameters for Probit Regression: With Application to Swedish Job Search Data

In ridge regression, the estimation of the ridge parameter is an important issue. This article generalizes some methods for estimating the ridge parameter for probit ridge regression (PRR) model based on the work of Kibria et al. (2011). The performance of these new estimators is judged by calculating the mean squared error (MSE) using Monte Carlo simulations. In the design of the experiment, we chose to vary the sample size and the number of regressors. Furthermore, we generate explanatory variables that are linear combinations of other regressors, which is a common situation in economics. In an empirical application regarding Swedish job search data, we also illustrate the benefits of the new method.
Assessment of pain: a comparison between patients and doctors

In a trial based on 52 patients admitted because of acute abdominal pain, we compared the assessment of pain intensity by doctors and patients according to a visual analogue scale. We found significant differences with regard to the median score of the doctors (3.4 cm) compared to the patients (6.1 cm). The correlation was 0.64 while the differences in dispersion were insignificant. Our findings indicate that it is far from unimportant who makes the pain assessment – the patient who experiences it or the doctor who evaluates it – but they also suggest that the differences could be a matter of scaling.
Does Perceptual Belongingness Affect Lightness Constancy?

The relation between perceptual belongingness and lightness perception has historically been studied in the contrast domain (Benary, 1924 Psychologische Forschung 5 131–142). However, scientists have shown that two equal grey patches may differ in lightness when belonging to different reflecting surfaces. We extend this investigation to the constancy domain. In a CRT simulation of a bipartite field of illumination, we manipulated the arrangement of twelve patches: six squares and six diamonds. Patches of the same shape could be placed: (i) all within the same illumination field; or (ii) forming a row across the illumination fields. Furthermore, we manipulated proximity between the innermost patches and the illumination edge. The patches could be (i) touching (forming an X-junction); or (ii) not touching (not forming an X-junction). Observers were asked to perform a lightness match between two additional patches, one illuminated and the other in shadow. We found better lightness constancy when the patches of the same shape formed a row across the fields, with no effect of X-junctions. Since lightness constancy is improved by strengthening the belongingness across the illumination fields, we conclude that belongingness might help the visual system to aggregate the differently illuminated surfaces, and facilitate the scission process.
Clinicians' Perspectives on Negative Effects of Psychological Treatments

Negative effects of psychological treatments is a fairly unexplored area of clinical research. Previous investigations have indicated that a portion of all patients experience negative effects in terms of deterioration and various adverse events. Meanwhile, evidence suggests that many clinicians are untrained in identifying negative effects and unaware of the current research findings. The objective of the current study is thus to investigate clinicians' own perspectives and experiences of possible negative effects of psychological treatments. An invitation to participate in an anonymous online survey consisting of 14 open-ended questions was distributed via three mailing lists used by clinicians that primarily identify themselves as cognitive behavior therapists. The responses were analyzed using a qualitative method based on thematic analysis. In total, 74 participants completed the survey. A majority agreed that negative effects of psychological treatments exist and pose a problem, and many reported having experienced both deterioration and adverse events among patients in their own practice. The thematic analysis resulted in three core themes: characteristics of negative effects, causal factors, as well as methods and criteria for evaluating negative effects. The clinicians recognize that negative effects exist, but many are unaware of the current research findings and are unfamiliar with methods and criteria for identifying and preventing deterioration and adverse events. The results provide evidence for further dissemination of the present knowledge regarding negative effects, particularly during basic clinical training, as well as the need for raising awareness of the available methods for identifying and preventing negative effects.
Primary Care After Psychiatric Crisis: A Qualitative Analysis

PURPOSE Patients with serious psychiatric problems experience difficulty accessing primary care. The goals of this study were to assess whether care managers improved access and to understand patients’ experiences with health care after a psychiatric crisis. METHODS A total of 175 consecutive patients seeking care in a psychiatric emergency department were randomly assigned to an intervention group with care managers or a control group. Brief, semistructured interviews about health care encounters were conducted at baseline and 1 year later. Five raters, using the content-driven, immersion-crystallization approach, analyzed 112 baseline and year-end interviews from 28 participants in each group. The main outcomes were patients’ responses about their care experiences, connections with primary care, and integration of medical and mental health care. Scores for physical function and mental function were compared by analysis of variance (ANOVA). RESULTS At baseline, most participants described negative experiences in receiving care and emphasized the importance of listening, sensitivity, and respect. Fully 71% of patients in the intervention group said that having a care manager to assist them with primary care connections was beneficial. Patients in the intervention group had significantly better physical and mental function than their counterparts in the control group at 6 months (P = .03 for each) but not at 12 months. There was also a trend toward functional improvement over the course of the study in the intervention group. CONCLUSIONS This analysis suggests that care management is effective in helping patients access primary care after a psychiatric crisis. It provides evidence on and insight into how care may be delivered more effectively for this population. Future work should assess the sustainability of care connections and longer-term patient health outcomes.
The sample complexity of learning linear predictors with the squared loss

We provide a tight sample complexity bound for learning bounded-norm linear predictors with respect to the squared loss. Our focus is on an agnostic PAC-style setting, where no assumptions are made on the data distribution beyond boundedness. This contrasts with existing results in the literature, which rely on other distributional assumptions, refer to specific parameter settings, or use other performance measures.
Subjective Outcome Evaluation of the Project P.A.T.H.S. (Secondary 2 Program): Views of the Program Participants

A total of 196 secondary schools participated in the Secondary 2 Program of the Full Implementation Phase of the Project P.A. T.H. S. (Positive Adolescent Training through Holistic Social Programmes). After completion of the Tier 1 Program, 30,731 students responded to the Subjective Outcome Evaluation Form (Form A) to assess their perceptions of the program, instructors, and perceived effectiveness of the program. Based on the consolidated reports submitted by the schools to the funding body, the research team aggregated the consolidated data to form a “reconstructed” overall profile on the perceptions of the program participants. Findings demonstrated that high proportions of the respondents had positive perceptions of the program and the instructors, and roughly four-fifths of the respondents regarded the program as beneficial to them. Correlation analyses showed that perceived program and instructor characteristics were positively associated with perceived benefits of the program.