Miracle-dz/UniFAR-FTUs huggingface dataset:
Anion-Sensitive Regions of L-Type CaV1.2 Calcium Channels Expressed in HEK293 Cells

L-type calcium currents (ICa) are influenced by changes in extracellular chloride, but sites of anion effects have not been identified. Our experiments showed that CaV1.2 currents expressed in HEK293 cells are strongly inhibited by replacing extracellular chloride with gluconate or perchlorate. Variance-mean analysis of ICa and cell-attached patch single channel recordings indicate that gluconate-induced inhibition is due to intracellular anion effects on Ca2+ channel open probability, not conductance. Inhibition of CaV1.2 currents produced by replacing chloride with gluconate was reduced from ∼75%–80% to ∼50% by omitting β subunits but unaffected by omitting α2δ subunits. Similarly, gluconate inhibition was reduced to ∼50% by deleting an α1 subunit N-terminal region of 15 residues critical for β subunit interactions regulating open probability. Omitting β subunits with this mutant α1 subunit did not further diminish inhibition. Gluconate inhibition was unchanged with expression of different β subunits. Truncating the C terminus at AA1665 reduced gluconate inhibition from ∼75%–80% to ∼50% whereas truncating it at AA1700 had no effect. Neutralizing arginines at AA1696 and 1697 by replacement with glutamines reduced gluconate inhibition to ∼60% indicating these residues are particularly important for anion effects. Expressing CaV1.2 channels that lacked both N and C termini reduced gluconate inhibition to ∼25% consistent with additive interactions between the two tail regions. Our results suggest that modest changes in intracellular anion concentration can produce significant effects on CaV1.2 currents mediated by changes in channel open probability involving β subunit interactions with the N terminus and a short C terminal region.
Quantitative reflection contrast microscopy of living cells

Mammalian cells in culture (BHK-21, PtK2, Friend, human flia, and glioma cells) have been observed by reflection contrast microscopy. Images of cells photographed at two different wavelengths (546 and 436 nm) or at two different angles of incidence allowed discrimination between reflected light and light that was both reflected and modulated by interference. Interference is involved when a change in reflected intensity (relative to glass/medium background reflected intensity) occurs on changing either the illumination wavelength or the reflection incidence angle. In cases where interference occurs, refractive indices can be determined at points where the optical path difference is known, by solving the given interference equation. Where cells are at least 50 nm distant from the glass substrate, intensities are also influenced by that distance as well as by the light's angle of incidence and wavelength. The reflected intensity at the glass/medium interface is used as a standard in calculating the refractive index of the cortical cytoplasm. Refractive indices were found to be higher (1.38--1.40) at points of focal contact, where stress fibers terminate, than in areas of close contact (1.354--1.368). In areas of the cortical cytoplasm, between focal contacts, not adherent to the glass substrate, refractive indices between 1.353 and 1.368 were found. This was thought to result from a microfilamentous network within the cortical cytoplasm. Intimate attachment of cells to their substrate is assumed to be characterized by a lack of an intermediate layer of culture medium.
Self-employment grants vs. subsidized employment: Is there a difference in the re-unemployment risk?

Self-employment grants and employment subsidies are active labor market programs that aim at helping unemployed workers to escape unemployment by becoming self-employed or being hired at an initially reduced cost for the employer. In Sweden in the 1990's the participation rate in the self-employment program increased from virtually none to almost same as in the employment subsidy program. The advancement of the self-employment program is likely to be a result of (i) a change in the labor market program policy, and (ii) an increase in the supply of skilled unemployed workers. The justification for the policy change is unclear, however. The literature indicate that a rather specific group of unemployed workers may benefit from self-employment programs; Neither are there any strong reasons to believe in general that self-employment should be preferable to conventional employment through subsidies. We examine, ex post, the justification for the policy change by comparing the post-program duration of employment for the two programs. In addition, we focus in some detail on the outcome for female workers and workers of foreign citizenship. The reason for this is the explicit policy to direct those workers to self-employment. The data we study are the inflow to the two programs from June 1995 to December 1996. The program participants are followed to March 1999. The data contain detailed spell and background information on 9,043 unemployed workers who participated in the self-employment program and 14,142 who participated in the employment subsidy program. The second explanation, see (ii), for the increase in self-employment program implies a potentially serious selection problem. We discuss how the selection process may bias the effect estimate in the non-linear duration model that we use. Simulations help us to determine the magnitude of the selection bias in our application. Moreover, we exploit the existing behavioral heterogeneity across labor market offices to reduce the selection bias. We find that the risk of re-unemployment is more than twice as high for the subsidized employment program compared with the self-employment program. The large positive effect is, however, limited to male and female workers of Swedish origin. We thus conclude that the policy change in general has been successful, though we note that directing immigrant workers to self-employment is unlikely to improve the situation for this group of unfortunate workers on the Swedish labor market.
Assisted reproductive technology and risk of asthma and allergy in the offspring: protocol for a systematic review and meta-analysis

Introduction The use of assisted reproductive technology (ART) procedures has increased globally over the last three decades. Recent observational studies suggest that children conceived through ART may be at increased risk of asthma and atopic disease compared with children conceived naturally, but findings are mixed. We aim to synthesise the evidence on the impact of ART on the risk of asthma and atopic disease in the offspring. Methods and analysis We will identify relevant studies by searching MEDLINE, EMBASE, Cochrane Library, ISI Web of Science, CINAHL, Scopus, Google Scholar, AMED, Global Health, PsychINFO, CAB International and the WHO Global Health Library from 1978 to 2016. We will locate additional studies through searching databases of the proceedings of international conferences, contacting international experts in the field, and searching the references cited in identified studies. We will include analytic observational studies (cohort studies, case–control studies and cross-sectional studies) that have investigated the impact of any type of ART on offspring's asthma and atopic disease. Screening of identified records, data extraction from eligible studies and risk of bias assessment of eligible studies will be independently undertaken by two reviewers, with arbitration by a third reviewer. The Effective Public Health Practice Project will be employed for risk of bias assessment. Estimates from studies judged to be clinically, methodologically and statistically homogeneous will be synthesised using random-effects meta-analysis. Ethics and dissemination As this study is based solely on the published literature, no ethics approval is required. We will publish our findings in a peer-reviewed scientific journal and present the results at national and international scientific conferences. Protocol registration We will register a detailed protocol for the review with the International Prospective Register of Systematic Reviews (PROSPERO) prior to starting the review.
Explanation of Two Anomalous Results in Statistical Mediation Analysis

Previous studies of different methods of testing mediation models have consistently found two anomalous results. The first result is elevated Type I error rates for the bias-corrected and accelerated bias-corrected bootstrap tests not found in nonresampling tests or in resampling tests that did not include a bias correction. This is of special concern as the bias-corrected bootstrap is often recommended and used due to its higher statistical power compared with other tests. The second result is statistical power reaching an asymptote far below 1.0 and in some conditions even declining slightly as the size of the relationship between X and M, a, increased. Two computer simulations were conducted to examine these findings in greater detail. Results from the first simulation found that the increased Type I error rates for the bias-corrected and accelerated bias-corrected bootstrap are a function of an interaction between the size of the individual paths making up the mediated effect and the sample size, such that elevated Type I error rates occur when the sample size is small and the effect size of the nonzero path is medium or larger. Results from the second simulation found that stagnation and decreases in statistical power as a function of the effect size of the a path occurred primarily when the path between M and Y, b, was small. Two empirical mediation examples are provided using data from a steroid prevention and health promotion program aimed at high school football players (Athletes Training and Learning to Avoid Steroids; Goldberg et al., 1996), one to illustrate a possible Type I error for the bias-corrected bootstrap test and a second to illustrate a loss in power related to the size of a. Implications of these findings are discussed.
Steepest Descent and Conjugate Gradient Methods with Variable Preconditioning

We analyze the conjugate gradient (CG) method with variable preconditioning for solving a linear system with a real symmetric positive definite (SPD) matrix of coefficients $A$. We assume that the preconditioner is SPD on each step, and that the condition number of the preconditioned system matrix is bounded above by a constant independent of the step number. We show that the CG method with variable preconditioning under this assumption may not give improvement, compared to the steepest descent (SD) method. We describe the basic theory of CG methods with variable preconditioning with the emphasis on “worst case” scenarios, and provide complete proofs of all facts not available in the literature. We give a new elegant geometric proof of the SD convergence rate bound. Our numerical experiments, comparing the preconditioned SD and CG methods, not only support and illustrate our theoretical findings, but also reveal two surprising and potentially practically important effects. First, we analyze variable preconditioning in the form of inner-outer iterations. In previous such tests, the unpreconditioned CG inner iterations are applied to an artificial system with some fixed preconditioner as a matrix of coefficients. We test a different scenario, where the unpreconditioned CG inner iterations solve linear systems with the original system matrix $A$. We demonstrate that the CG-SD inner-outer iterations perform as well as the CG-CG inner-outer iterations in these tests. Second, we compare the CG methods using a two-grid preconditioning with fixed and randomly chosen coarse grids, and observe that the fixed preconditioner method is twice as slow as the method with random preconditioning.
Doc2Sent2Vec: A Novel Two-Phase Approach for Learning Document Representation

Doc2Sent2Vec is an unsupervised approach to learn low-dimensional feature vector (or embedding) for a document. This embedding captures the semantics of the document and can be fed as input to machine learning algorithms to solve a myriad number of applications in the field of data mining and information retrieval. Some of these applications include document classification, retrieval, and ranking. The proposed approach is two-phased. In the first phase, the model learns a vector for each sentence in the document using a standard word-level language model. In the next phase, it learns the document representation from the sentence sequence using a novel sentence-level language model. Intuitively, the first phase captures the word-level coherence to learn sentence embeddings, while the second phase captures the sentence-level coherence to learn document embeddings. Compared to the state-of-the-art models that learn document vectors directly from the word sequences, we hypothesize that the proposed decoupled strategy of learning sentence embeddings followed by document embeddings helps the model learn accurate and rich document representations. We evaluate the learned document embeddings by considering two classification tasks: scientific article classification and Wikipedia page classification. Our model outperforms the current state-of-the-art models in the scientific article classification task by ? 12.07% and the Wikipedia page classification task by ? 6.93%, both in terms of F1 score. These results highlight the superior quality of document embeddings learned by the Doc2Sent2Vec approach.
Making Sense of the 1980s and 1990s

This paper describes and considers explanations for changes in corporate governance and merger activity in the United States since 1980. Corporate governance in the 1980s was dominated by intense merger activity distinguished by the prevalence of leveraged buyouts (LBOs) and hostility. After a brief decline in the early 1990s, substantial merger activity resumed in the second half of the decade, while LBOs and hostility did not. Instead, internal corporate governance mechanisms appear to have played a larger role in the 1990s. We conclude by considering whether these changes and the movement toward shareholder value are likely to be permanent.
Lack of experience-based stratification in homing pigeon leadership hierarchies

In societies that make collective decisions through leadership, a fundamental question concerns the individual attributes that allow certain group members to assume leadership roles over others. Homing pigeons form transitive leadership hierarchies during flock flights, where flock members are ranked according to the average time differences with which they lead or follow others' movement. Here, we test systematically whether leadership ranks in navigational hierarchies are correlated with prior experience of a homing task. We constructed experimental flocks of pigeons with mixed navigational experience: half of the birds within each flock had been familiarized with a specific release site through multiple previous releases, while the other half had never been released from the same site. We measured the birds' hierarchical leadership ranks, then switched the same birds' roles at a second site to test whether the relative hierarchical positions of the birds in the two subsets would reverse in response to the reversal in levels of experience. We found that while across all releases the top hierarchical positions were occupied by experienced birds significantly more often than by inexperienced ones, the remaining experienced birds were not consistently clustered in the top half—in other words, the network did not become stratified. We discuss our results in light of the adaptive value of structuring leadership hierarchies according to ‘merit’ (here, navigational experience).
Predicting Success in College: A Longitudinal Study of Achievement Goals and Ability Measures as Predictors of Interest and Performance From Freshman Year Through Graduation

The authors examined the role of achievement goals, ability, and high school performance in predicting academic success over students’ college careers. First, the authors examined which variables predicted students’ interest and performance in an introductory psychology course taken their first semester in college. Then, the authors followed students until they graduated to examine continued interest in psychology and performance in subsequent classes. Achievement goals, ability measures, and prior high school performance each contributed unique variance in predicting initial and long-term outcomes, but these predictors were linked to different educational outcomes. Mastery goals predicted continued interest, whereas performance-approach goals predicted performance. Ability measures and prior high school performance predicted academic performance but not interest. The findings support a multiple goals perspective.
RASIONAL DAN ELEMEN PERUBAHAN KURIKULUM 2013

Kurikulum 2013 merupakan pengembangan Kurikulum Berbasis Kompetensi yang telah dirintis pada tahun 2004 dan KTSP 2006. Para guru harus memiliki kesiapan yang memadai dan matang, mulai dari kesiapan dari segi kualifikasi, kompetensi serta juga siap dalam hal kesamaan pemahaman dan mindset (pola pikir) terhadap kurikulum baru tersebut. Proses pembelajaran yang mendorong siswa untuk aktif hanya mungkin terwujud bila mindset guru telah berubah, telah berjalan sesuai dengan arah dan perubahan Kurikulum 2013; Kegagalan mengubah mindset guru akan menjadi sumber kegagalan implementasi Kurikulum 2013. Persoalannya adalah bahwa perubahan mindset guru tidak bisa dilakukan dalam waktu singkat; Tulisan ini membahas 3 hal terkait dengan implementasi kurikulum 2013: rasional, eleman dan perlunya perubahan mindset guru dalam implementasinya.
Gender differences in parasympathetic reactivation during recovery from Wingate anaerobic test

Parasympathetic reactivation from a 30-s Wingate anaerobic test was investigated in 16 male and 15 female volunteers. Heart rate recovery was assessed as the difference between peak exercise heart rate and the heart rate recorded following 60 seconds of recovery (HRR60). The time constant of the first 30 s post-exercise HR (T30) was determined as a negative reciprocal of the slope of the regression line. Time constant decay (T) was obtained by fitting the 5 minute post-exercise HRR into a first-order exponential curve. Heart rate variability (HRV) was used to describe changes in autonomic cardiac regulation. Post exercise heart rate recovery was faster in male participants, demonstrated through HRR60 (29.5±8.9 vs. 23.4±9.8 seconds respectively) and T30 (292.4±88.7 vs. 409.2±138.3 seconds respectively), but the time constant decay of heart rate (T) did not differ between the two genders. The time course of RMSSD30 recovery was impaired immediately after exercise. None of the observed vagal HRV indices have restored after five minutes of recovery following the 30-s exercise, but the post-exercise lnHF2-5min was significantly smaller in females (3.3±0.9 ms2 in males vs. 2.5±1.0 ms2 in females). The immediate HRR and parasympathetic reactivation was affected by gender and was attenuated in female participants.
Open-set Plant Identification Using an Ensemble of Deep Convolutional Neural Networks

Open-set recognition, a challenging problem in computer vision, is concerned with identification or verification tasks where queries may belong to unknown classes. This work describes a fine-grained plant identification system consisting of an ensemble of deep convolutional neural networks within an open-set identification framework. Two wellknown deep learning architectures of VGGNet and GoogLeNet, pretrained on the object recognition dataset of ILSVRC 2012, are finetuned using the plant dataset of LifeCLEF 2015. Moreover, GoogLeNet is fine-tuned using plant and non-plant images for rejecting samples from non-plant classes. Our systems have been evaluated on the test dataset of PlantCLEF 2016 by the campaign organizers and our best proposed model has achieved an official score of 0.738 in terms of the mean average precision, while the best official score is 0.742.
Efficacy of catheter ablation and surgical CryoMaze procedure in patients with long-lasting persistent atrial fibrillation and rheumatic heart disease: a randomized trial.

AIMS Catheter ablation and surgical Maze procedure are effective in treating atrial fibrillation (AF) patients. However, there is no study that compares the effect of circumferential pulmonary vein isolation (CPVI) combined with substrate ablation after valvular surgery and the concomitant Maze procedure for the treatment of AF in patients with rheumatic heart disease (RHD). The aim of this study was to compare the effectiveness of CPVI combined with substrate modification and surgical Maze procedure using Saline-Irrigated Cooled-tip Radiofrequency Ablation (SICTRA) system for the treatment of long-lasting persistent AF in patients with RHD. METHODS AND RESULTS Between January 2006 and June 2008, 99 patients with long-lasting persistent AF and RHD were randomly assigned to undergo valvular operation and CPVI combined with substrate modification 6 months after the surgery (Group A, 49 patients) or valvualr operation and concomitant Maze procedure (Group B, 50 patients). The mean follow-up periods were 15 ± 5 and 20 ± 8 months in Groups A and B, respectively. After one procedure, Group B had a significantly higher freedom from artial arrhythmias compared with Group A (82% in Group B vs. 55.2% in Group A, P < 0.001). Fifteen patients in Group A underwent a redo procedure. Six patients in Group B underwent catheter ablation and four were treated successfully. The cumulative rates of sinus rhythm were 71% in Group A and 88% in Group B (P < 0.001). CONCLUSION The concomitant Cox Maze procedure using SICTRA is more effective than subsequent CPVI combined with substrate modification in treating patients with long-lasting persistent AF and RHD.
Motivated Attention and Reward in Parenting

SYNOPSIS Parenting has a significant and lasting impact on child development. From birth, parents must sensitively and appropriately attend to their infant’s emotional expressions and vocalizations. Accumulating evidence indicates that these infant cues of emotion attract more attention than equivalent adult cues in parents as well as non-parents. We review this evidence and suggest that infant cues hold high incentive value and elicit motivated attention (i.e., enhanced processing of motivationally relevant stimuli), which in turn promotes approach motivation and thus caregiving responses. Further, we discuss data suggesting that infant cues are salient for non-parents, with increasing motivated attention to infant cues in the transition to parenthood. This increase may depend on interactions between the dopamine reward system and the neuropeptide oxytocin. Therefore, we also explore the human and non-human data that support this association and consider potential sources of variability in motivated attention in parents.
Semi-autonomous visual inspection of vessels assisted by an unmanned Micro Aerial Vehicle

Vessel maintenance entails periodic visual inspections of internal and external parts of the hull in order to detect the typical defective situations affecting metallic structures, such as cracks, coating breakdown, corrosion, etc. The main goal of the EU-FP7 project MINOAS is the automation of the inspection process, currently undertaken by human surveyors, by means of a fleet of robotic agents. This paper overviews a semi-autonomous approach to the inspection problem consisting of an autonomous Micro Aerial Vehicle (MAV) to be used as part of this fleet and which is in charge of regularly supplying images that can effectively teleport the surveyor from a base station to the areas of the hull to be inspected. Specific image processing software to analyze those images and assist the surveyor during the repair/no repair decision making process is also contributed. The control software approach adopted for the MAV, including self-localization and obstacle avoidance, is described and discussed, and experimental results in this regard are as well reported.