Miracle-dz/UniFAR-FTUs huggingface dataset:
Deletion of Monoglyceride Lipase in Astrocytes Attenuates Lipopolysaccharide-induced Neuroinflammation*

Monoglyceride lipase (MGL) is required for efficient hydrolysis of the endocannabinoid 2-arachidonoylglyerol (2-AG) in the brain generating arachidonic acid (AA) and glycerol. This metabolic function makes MGL an interesting target for the treatment of neuroinflammation, since 2-AG exhibits anti-inflammatory properties and AA is a precursor for pro-inflammatory prostaglandins. Astrocytes are an important source of AA and 2-AG, and highly express MGL. In the present study, we dissected the distinct contribution of MGL in astrocytes on brain 2-AG and AA metabolism by generating a mouse model with genetic deletion of MGL specifically in astrocytes (MKOGFAP). MKOGFAP mice exhibit moderately increased 2-AG and reduced AA levels in brain. Minor accumulation of 2-AG in the brain of MKOGFAP mice does not cause cannabinoid receptor desensitization as previously observed in mice globally lacking MGL. Importantly, MKOGFAP mice exhibit reduced brain prostaglandin E2 and pro-inflammatory cytokine levels upon peripheral lipopolysaccharide (LPS) administration. These observations indicate that MGL-mediated degradation of 2-AG in astrocytes provides AA for prostaglandin synthesis promoting LPS-induced neuroinflammation. The beneficial effect of astrocyte-specific MGL-deficiency is not fully abrogated by the inverse cannabinoid receptor 1 agonist SR141716 (Rimonabant) suggesting that the anti-inflammatory effects are rather caused by reduced prostaglandin synthesis than by activation of cannabinoid receptors. In conclusion, our data demonstrate that MGL in astrocytes is an important regulator of 2-AG levels, AA availability, and neuroinflammation.
Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1

Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modular designs, M. leprae PBP1 is related to Escherichia coli PBP1a and PBP1b, bienzymatic proteins with transglycosylase and transpeptidase activities. When produced in E. coli, His tag-labelled derivatives of M. leprae PBP1 adopt the correct membrane topology, with the bulk of the polypeptide chain on the surface of the plasma membrane. They defy attempts at solubilization with all the detergents tested except cetyltrimethylammonium bromide. The solubilized PBP1 derivatives can be purified by affinity chromatography on Ni2+-nitrilotriacetic acid agarose. They have low affinities for the usual penicillins and cephalosporins.
Matrix Completion from $O(n)$ Samples in Linear Time

We consider the problem of reconstructing a rank-$k$ $n \times n$ matrix $M$ from a sampling of its entries. Under a certain incoherence assumption on $M$ and for the case when both the rank and the condition number of $M$ are bounded, it was shown in \cite{CandesRecht2009, CandesTao2010, keshavan2010, Recht2011, Jain2012, Hardt2014} that $M$ can be recovered exactly or approximately (depending on some trade-off between accuracy and computational complexity) using $O(n \, \text{poly}(\log n))$ samples in super-linear time $O(n^{a} \, \text{poly}(\log n))$ for some constant $a \geq 1$. In this paper, we propose a new matrix completion algorithm using a novel sampling scheme based on a union of independent sparse random regular bipartite graphs. We show that under the same conditions w.h. p. our algorithm recovers an $\epsilon$-approximation of $M$ in terms of the Frobenius norm using $O(n \log^2(1/\epsilon))$ samples and in linear time $O(n \log^2(1/\epsilon))$. This provides the best known bounds both on the sample complexity and computational complexity for reconstructing (approximately) an unknown low-rank matrix. The novelty of our algorithm is two new steps of thresholding singular values and rescaling singular vectors in the application of the "vanilla" alternating minimization algorithm. The structure of sparse random regular graphs is used heavily for controlling the impact of these regularization steps.
Efeitos contextuais no acesso lexical de palavras polissêmicas e homônimas 1 Contextual effects on lexical access of polysemic and homonym words

In this text we discuss how polysemous and homonyms words are accessed and what is the role of context during this access. Works by Swinney (1979) and Tanenhaus et al. (1979) support the hypothesis of multiple access, for which the context acts only late in the lexical access of ambiguous words. On the other hand, Tabossi and Zardon (1993) and Simpson (1994) support the hypothesis of selective access, according to which only the sense specified by context is accessed during the reading of an ambiguous word. To check the validity of these assumptions in the access of polysemous and homonyms words, we conducted three experiments of cross-modal priming. The results corroborate the hypothesis of multiple access. Regarding the relationship between polysemy and homonymy, our findings provide evidence for the hypothesis that polysemous and homonyms words are accessed in the same way, even if there are more senses associated with polysemies diachronically.
Role Reversal: Educators in an Enabling Program Embark on a Journey of Critical Self-Reflection.

While much has been written about the transformative potential of adult education from the student perspective, little research has been done into the experiences of those who teach in such contexts. This paper draws on the reflections of three academics who work in an enabling program in regional Australia. We embarked on a process of critical self-reflection, not unlike the kind of activity we ask of our students, as we considered the challenges as well as the rewards inherent in our experiences. Brookfield’s (1998) concept of critical reflection was used as a vehicle for examining underlying assumptions about what we do and how we are positioned within the broader University community. Though we are cautious about describing our learning as ‘transformative’, we contend that the process of critical reflection has led to a heightened awareness, and has enabled us to articulate perceptions of professional and personal growth.
Flow Dimensions on Daily Activities with the Spanish Version of the Flow Scale (DFS)

Abstract A sample of 250 students of psychology with an average age of 20.37 years, answered the Flow Q questionnaire indicating their favorite flow activity, and the Spanish version of the Dispositional Flow Scale (DFS). A confirmatory factor analysis assessed the DFS construct validity of the flow model on daily activities. Both a hierarchical model of eight first order factors reflecting a second order global flow factor, and a model with eight formative first order flow dimensions, showed good fit and discriminant power. Most optimal activities were found to be individual and structured, such as studying, reading and certain forms of individual sports. Leisure activities turned out to be more rewarding than studying. Sports displayed more flow, clear goals, merging of action and awareness, and autotelic experience. Reading also showed more flow, balance of challenge and skills, feedback, merging of action and awareness, and loss of self-consciousness. On the other hand, studying displayed less flow, merging of action and awareness, and autotelic experience.
Counselling psychologists' talk about the diagnosis of 'schizophrenia'

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Structures of Human Cytosolic NADP-dependent Isocitrate Dehydrogenase Reveal a Novel Self-regulatory Mechanism of Activity*

Isocitrate dehydrogenases (IDHs) catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate, and regulation of the enzymatic activity of IDHs is crucial for their biological functions. Bacterial IDHs are reversibly regulated by phosphorylation of a strictly conserved serine residue at the active site. Eukaryotic NADP-dependent IDHs (NADP-IDHs) have been shown to have diverse important biological functions; however, their regulatory mechanism remains unclear. Structural studies of human cytosolic NADP-IDH (HcIDH) in complex with NADP and in complex with NADP, isocitrate, and Ca2+ reveal three biologically relevant conformational states of the enzyme that differ substantially in the structure of the active site and in the overall structure. A structural segment at the active site that forms a conserved α-helix in all known NADP-IDH structures assumes a loop conformation in the open, inactive form of HcIDH; a partially unraveled α-helix in the semi-open, intermediate form; and an α-helix in the closed, active form. The side chain of Asp279 of this segment occupies the isocitrate-binding site and forms hydrogen bonds with Ser94 (the equivalent of the phosphorylation site in bacterial IDHs) in the inactive form and chelates the metal ion in the active form. The structural data led us to propose a novel self-regulatory mechanism for HcIDH that mimics the phosphorylation mechanism used by the bacterial homologs, consistent with biochemical and biological data. This mechanism might be applicable to other eukaryotic NADP-IDHs. The results also provide insights into the recognition and specificity of substrate and cofactor by eukaryotic NADP-IDHs.
Bases cérébrales de la perception auditive simple et complexe dans l’autisme

Perception involves the processes allowing the brain to extract and understand sensory information. Atypical perceptual processing has been associated with the autistic phenotype usually described in terms of impairments in social and communication abilities, as well as restricted interests and repetitive behaviours. Perceptual atypicalities are reported across a range of tasks. For instance, superior performance in autistics compared to non autistics is observed for pure tone discrimination as well as for complex figure disembodying tasks. One particular study reported atypical low-level visual processing in autism. In this experiment, autistics displayed enhanced performance for identifying the orientation of luminance-defined gratings and inferior performance for texture-defined gratings in comparison to non autistics. This dichotomous pattern led to the formulation of a hypothesis suggesting an inverse relation between the level of performance and the extent (or complexity) of the cortical network required for processing the stimuli. Specifically, autistics would perform better than non autistics during processing visual stimuli involving one cortical region (luminance-defined or simple stimuli), while they would show decreased performance for processing stimuli involving a network of cortical region (texture-defined or complex stimuli). Atypical perceptual processing is described as a general feature associated with the autistic phenotype and is reported for both the visual and the auditory modalities. Considering the existing parallels between the two sensory modalities, the principal purpose of the presented doctoral dissertation it to verify whether the hypothesis proposed to explain atypical visual processing in autism could also apply to audition. The first article (Chapter 2) is an exhaustive literature review of studies on autistics’ auditory processing abilities. Taken together, the results suggest that the level of performance of autistics on auditory tasks could be related to the acoustic complexity of the stimuli.
Electroencephalographic responses to neck cut and exsanguination in minimally anaesthetized goats.

Conscious animals typically experience sensory (nociception) and emotional pain, whereas unconscious animals that were minimally anesthetized would experience minimal emotional pain. To determine whether ‘silencing’ the emotional component through a minimally anesthetized model would minimize stress response, and thus improve animal welfare, this study aimed at comparing changes in electroencephalographic (EEG) activities associated with possible noxious stimuli following neck-cut slaughter in conscious non-anesthetized versus minimally anaesthetized Boer cross-bred goats. Ten bucks were randomly assigned to two groups of five animals each, and subjected to neck-cut slaughter when fully conscious (HS) or under minimal anaesthesia (AS) and exsanguinated. The anaesthesia was induced with propofol (5 mg/kg) administered to effect by rapid injection into a cephalic vein and maintained with halothane in 100 % oxygen. Changes in the root mean square (RMS) for each of alpha, beta, delta and theta waves, median frequency (F50) and total power of the EEG (Ptot) were compared in each group before and after neck cut and between groups following treatments. Electroencephalographic parameters did not differ between goats that were fully conscious or slaughtered under minimal anaesthesia. These findings showed that the noxious stimuli from neck cut were present in both conscious and minimally anaesthetized goats. Most importantly, the presence of emotional pain and nociception did not affect the extent of electroencephalographic responses significantly compared with animals that were experiencing nociception only. Keywords : brain activity, propofol-halothane anaesthetic, noxious stimuli, slaughter
The place of closed manipulation in the management of flexion-rotation dislocations of the cervical spine.

1. The pathology and pathogenesis of dislocations and fracture-dislocations of the cervical spine has been reviewed. 2. A method of treatment using skeletal traction and manipulation under relaxant general anaesthesia is described. Results of treatment are given for all patients admitted to the Centre with flexion-rotation dislocations of the cervical spine complicated by neurological lesions, between November 1961 and December 1968. 3. After reviewing the literature and considering the results obtained in seventy-six cases, we advocate a policy of conservative management with gentle manipulation of the cervical spine in selected cases, reduction being maintained thereafter by skeletal traction. We reserve operation for the few cases that demonstrate late instability or for those rarer cases in which manipulation fails and the patient has either an incomplete neurological lesion or a double skeletal injury. 4. The low incidence of late instability after adequate conservative treatment is stressed, and the danger of overdistraction of the cervical spine by heavy traction in patients with severe ligamentous damage is emphasised.
Palliative care services for those with chronic lung disease

Excellent palliative care is available for patients with advanced lung cancer. Whether the same services are available for those with nonmalignant respiratory disease is less clear. A questionnaire was sent to 210 named respiratory physicians, each representing a major hospital in England, Wales, and Northern Ireland. A total of 107 replies were received; the response rate was 51.0%. Respondents cared for patients with chronic obstructive pulmonary disease, asbestosis, and diffuse parenchymal lung disease but only a third had responsibility for cystic fibrosis. Physicians were supported by a mean of 3.4 respiratory nurse specialists per department and 73.8% had a specialist lung cancer nurse. In only 16 cases (20.3%) did that nurse extend care to those with nonmalignant disease. Only a minority reported easy access to hospice in-patient care or day care. About 21.5% of the respondents had formal policies in place for care of patients with chronic respiratory disease nearing the end of life, but 87.9% of respondents had no formal process for initiating end of life discussions with those with terminal respiratory illness. Patients with advanced nonmalignant respiratory disease have less universal access to specialist palliative care services than do those with malignant lung disease, and in the majority of hospitals there is no formalized approach to end of life care issues with patients with chronic lung disease.
Revising partially ordered beliefs

This paper deals with the revision of partially ordered beliefs. It proposes a semantic representation of epistemic states by partial pre-orders on interpretations and a syntactic representation by partially ordered belief bases. Two revision operations, the revision stemming from the history of observations and the possibilistic revision, defined when the epistemic state is represented by a total pre-order, are generalized, at a semantic level, to the case of a partial pre-order on interpretations, and at a syntactic level, to the case of a partially ordered belief base. The equivalence between the two representations is shown for the two revision operations.
Mycobacterium abscessus Morphotype Comparison in a Murine Model

Pulmonary infections with Mycobacterium abscessus (M. abscessus) are increasingly prevalent in patients with lung diseases such as cystic fibrosis. M. abscessus exists in two morphotypes, smooth and rough, but the impact of morphotype on virulence is unclear. We developed an immune competent mouse model of pulmonary M. abscessus infection and tested the differences in host inflammatory response between the morphotypes of M. abscessus. Smooth and rough morphotypes of M. abscessus were isolated from the same American Type Culture Collection strain. Wild type and cystic fibrosis mice were intratracheally inoculated with known quantities of M. abscessus suspended in fibrin plugs. At the time of sacrifice lung and splenic tissues and bronchoalveolar lavage fluid were collected and cultured. Bronchoalveolar lavage fluid was analyzed for leukocyte count, differential and cytokine expression. Pulmonary infection with M. abscessus was present at both 3 days and 14 days post-inoculation in all groups at greater levels than systemic infection. Inoculation with M. abscessus rough morphotype resulted in more bronchoalveolar lavage fluid neutrophils compared to smooth morphotype at 14 days post-inoculation in both wild type (p = 0.01) and cystic fibrosis (p<0.01) mice. Spontaneous in vivo conversion from smooth to rough morphotype occurred in 12/57 (21%) of mice. These mice trended towards greater weight loss than mice in which morphotype conversion did not occur. In the described fibrin plug model of M. abscessus infection, pulmonary infection with minimal systemic dissemination is achieved with both smooth and rough morphotypes. In this model M. abscessus rough morphotype causes a greater host inflammatory response than the smooth based on bronchoalveolar lavage fluid neutrophil levels.
Influence of Chlorine Substituents on Rates of Oxidation of Chlorinated Biphenyls by the Biphenyl Dioxygenase ofBurkholderia sp. Strain LB400

ABSTRACT Biphenyl dioxygenase from Burkholderia(Pseudomonas) sp. strain LB400 catalyzes the first reaction of a pathway for the degradation of biphenyl and a broad range of chlorinated biphenyls (CBs). The effect of chlorine substituents on catalysis was determined by measuring the specific activity of the enzyme with biphenyl and 18 congeners. The catalytic oxygenase component was purified and incubated with individual CBs in the presence of electron transport proteins and cofactors that were required for enzyme activity. The rate of depletion of biphenyl from the assay mixture and the rate of formation ofcis-biphenyl 2,3-dihydrodiol, the oxidation product, were almost equal, indicating that the assay accurately measured enzyme-specific activity. Four classes of CBs were defined based on their oxidation rates. Class I contained 3-CB and 2,5-CB, which gave rates that were approximately twice that of biphenyl. Class II contained 2,5,3′,4′-CB, 2,3,2′,5′-CB, 2,3,4,5-CB, 2,3,2′,3′-CB, 2,4,5,2′,5′-CB, 2,5,3′-CB, 2,5,4′-CB, 2-CB, and 3,4,5-CB, which gave rates that ranged from 97 to 35% of the biphenyl rate. Class III contained only 2,3,4,2′,5′-CB, which gave a rate that was 4% of the biphenyl rate. Class IV contained 2,4,4′-CB, 2,4,2′,4′-CB, 3,4,5,2′-CB, 3,4,5,3′-CB, 3,5,3′,5′-CB, and 3,4,5,2′,5′-CB, which showed no detectable depletion. Rates were not significantly correlated with the aqueous solubilities of the CBs or the number of chlorine substituents on the rings. Oxidation products were detected for all class I, II, and III congeners and were identified as chlorinatedcis-dihydrodiols for classes I and II. The specificity of biphenyl dioxygenase for the CBs examined in this study was determined by the relative positions of the chlorine substituents on the aromatic rings rather than the number of chlorine substituents on the rings.
Full Dimension MIMO (FD-MIMO): Demonstrating Commercial Feasibility

Massive multi-input multi-output (MIMO) is shown to significantly increase spectral efficiency by exploiting a large number of antennas to support high-order multiuser MIMO. In 3GPP Release-13, a full-dimension MIMO (FD-MIMO) technology was introduced to address practical aspects for massive MIMO in cellular systems; extensive simulations show 2–4 times capacity gain compared with current LTE systems. FD-MIMO has been identified as one of the key 5G technologies and is being continuously improved in 3GPP new radio standards. However, several practical challenges such as interference mitigation among MIMO streams for a large number of users with limited channel feedback, hardware limitations, such as calibration errors that limit the precoding capabilities need to be addressed carefully. A proof of concept (PoC) base-station and user equipment (UE) prototype has been designed to validate the potential of FD-MIMO technology and demonstrate commercial implementation feasibility. In this paper, we present theory and architecture behind an FD-MIMO prototype and share the field test results with LTE-based UEs in a multi-user MIMO setup. We also analyze the impact of transmitter and receiver calibration errors on the performance of the FD-MIMO system.
A simple upper bound on the capacity of BI-AWGN channel

The capacity of binary input additive white Gaussian noise (BI-AWGN) channel has no closed-form solution due to the complicated numerical integrations involved. In this letter, a simple upper bound to evaluate the capacity of BI-AWGN channel is presented. In addition, through the moment generating function (MGF) of fading channel gain, the upper bounds of fading channels are derived by averaging the proposed bound with respect to the probability density function (pdf ) of fading gain.