Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic characterization of p27kip1 and stathmin in controlling cell proliferation in vivo

The CDK inhibitor p27kip1 is a critical regulator of cell cycle progression, but the mechanisms by which p27kip1 controls cell proliferation in vivo are still not fully elucidated. We recently demonstrated that the microtubule destabilizing protein stathmin is a relevant p27kip1 binding partner. To get more insights into the in vivo significance of this interaction, we generated p27kip1 and stathmin double knock-out (DKO) mice. Interestingly, thorough characterization of DKO mice demonstrated that most of the phenotypes of p27kip1 null mice linked to the hyper-proliferative behavior, such as the increased body and organ weight, the outgrowth of the retina basal layer and the development of pituitary adenomas, were reverted by co-ablation of stathmin. In vivo analyses showed a reduced proliferation rate in DKO compared to p27kip1 null mice, linked, at molecular level, to decreased kinase activity of CDK4/6, rather than of CDK1 and CDK2. Gene expression profiling of mouse thymuses confirmed the phenotypes observed in vivo, showing that DKO clustered with WT more than with p27 knock-out tissue. Taken together, our results demonstrate that stathmin cooperates with p27kip1 to control the early phase of G1 to S phase transition and that this function may be of particular relevance in the context of tumor progression.
The role of pharmacotherapy in mild to moderate chronic obstructive pulmonary disease

Chronic obstructive pulmonary disease (COPD) is a major health problem worldwide and most of those afflicted have mild to moderate disease as measured by spirometry. There is mounting evidence that even mild airway obstruction is associated with activity-related dyspnea, exercise limitation, impaired quality of life, increased hospitalization and mortality. As our understanding of the complex, heterogeneous pathophysiology and clinical consequences of milder COPD continues to grow, there is increasing interest in the potential impact of therapeutic interventions beyond smoking cessation. Unfortunately, few clinical trials have included patients with mild to moderate disease and the evidence base for pharmacological treatment in this subpopulation is currently lacking. Recent short-term mechanistic studies confirm that reversal of airway smooth muscle cholinergic tone consistently improves respiratory mechanics during rest and exercise in mild COPD but long-term clinical benefits remain to be evaluated. Secondary analysis of large, prospective studies designed to evaluate the efficacy of long-acting bronchodilators, inhaled corticosteroids and combination therapy indicate that patients with moderate COPD achieve comparable benefits to those with advanced disease. In the absence of evidence-based guidelines for the management of milder COPD, treatment choices are driven mainly by clinical presentation: for those with persistent and troublesome activity-related dyspnea a trial of inhaled bronchodilator therapy is justified; for those with a propensity for recurrent infective exacerbations, consideration of additional anti-inflammatory treatment seems reasonable. In this paper, we review the current knowledge base and emerging paradigm for the pharmacological treatment of mild to moderate COPD.
Adaptive antennas at the mobile and base stations in an OFDM/TDMA system

Several smart antenna systems have been proposed and demonstrated at the base station (BS) of wireless communications systems, and these have shown that significant system performance improvement is possible. We consider the use of adaptive antennas at the BS and mobile stations (MS), operating jointly, in combination with orthogonal frequency-division multiplexing. The advantages of the proposed system includes reductions in average error probability and increases in capacity compared to conventional systems. Multiuser access, in space, time, and through subcarriers, is also possible and expressions for the exact joint optimal antenna weights at the BS and MS under cochannel interference conditions for fading channels are derived. To demonstrate the potential of our proposed system, analytical along with Monte Carlo simulation results are provided.
Electrically driven convection in a thin annular film undergoing circular Couette flow

We investigate the linear stability of a thin, suspended, annular film of conducting fluid with a voltage difference applied between its inner and outer edges. For a sufficiently large voltage, such a film is unstable to radially driven electroconvection due to charges which develop on its free surfaces. The film can also be subjected to a Couette shear by rotating its inner edge. This combination is experimentally realized using films of smectic A liquid crystals. In the absence of shear, the convective flow consists of a stationary, azimuthally one-dimensional (1D) pattern of symmetric, counter-rotating vortex pairs. When Couette flow is applied, an azimuthally traveling pattern results. When viewed in a co-rotating frame, the traveling pattern consists of pairs of asymmetric vortices. We calculate the neutral stability boundary for arbitrary radius ratio α and Reynolds number Re of the shear flow, and obtain the critical control parameter Rc(α,Re) and the critical azimuthal mode number mc(α,Re). The Co.
Trade Liberalisation and Poverty: What are the Links?

The following sections are included:BACKGROUNDTHE HOUSEHOLDPRICE CHANGES AND TRANSMISSIONFACTOR MARKETS: WAGES. EMPLOYMENT AND PROFITSTAXES AND SPENDINGSHOCKS, RISKS AND VULNERABILITYECONOMIC GROWTH AND TECHNOLOGYSHORT-TERM ADJUSTMENTTRADE LIBERALISATION AND POVERTY: A CHECKLISTACKNOWLEDGEMENTSREFERENCES
Neuronal Production of Lipocalin-2 as a Help-Me Signal for Glial Activation

Background and Purpose— We explored the hypothesis that injured neurons release lipocalin-2 as a help me signal. Methods— In vivo lipocalin-2 responses were assessed in rat focal cerebral ischemia and human stroke brain samples using a combination of ELISA and immunostaining. In vitro, microglia and astrocytes were exposed to lipocalin-2, and various markers and assays of glial activation were quantified. Functional relevance of neuron-to-glia lipocalin-2 signaling was examined by transferring conditioned media from lipocalin-2–activated microglia and astrocytes onto neurons to see whether activated glia could protect neurons against oxygen–glucose deprivation and promote neuroplasticity. Results— In human stroke samples and rat cerebral ischemia, neuronal expression of lipocalin-2 was significantly increased. In primary cell cultures, exposing microglia and astrocytes to lipocalin-2 resulted in glial activation. In microglia, lipocalin-2 converted resting ramified shapes into a long-rod morphology with reduced branching, increased interleukin-10 release, and enhanced phagocytosis. In astrocytes, lipocalin-2 upregulated glial fibrillary acid protein, brain-derived neurotropic factor, and thrombospondin-1. Conditioned media from lipocalin-2–treated astrocytes upregulated synaptotagmin, and conditioned media from lipocalin-2–treated microglia upregulated synaptophysin and post-synaptic density 95 (PSD95) and protected neurons against oxygen–glucose deprivation. Conclusions— These findings provide proof of concept that lipocalin-2 is released by injured neurons as a help me distress signal that activates microglia and astrocytes into potentially prorecovery phenotypes.
Association of enrolment in primary care networks with diabetes care and outcomes among First Nations and low-income Albertans

Background The prevalence of diabetes mellitus and its complications is higher among First Nations people and people with low socio-economic status (SES). Previous studies in Alberta have shown that provision of care through Primary Care Networks (PCNs) is associated with better quality of care and better outcomes for people with diabetes, possibly because of greater utilization of chronic disease management programs. However, it is unknown whether First Nations individuals and those in lower SES groups experience these benefits. Methods We used administrative and laboratory data for a population-based cohort analysis of Alberta residents under 65 years of age with diabetes. The primary outcome, assessed over a 1-year period, was admission to hospital or emergency department visit for a diabetes-specific ambulatory care sensitive condition (ACSC). Secondary outcomes were 2 quality-of-care indicators (likelihood of measurement of glycated hemoglobin [HbA1c] and or retinal screening) and 2 measures of health care utilization (visits to specialist and primary care physicians). We used negative binomial regression to determine the association between care within a PCN and hospital admission or emergency department visit for diabetes-specific ACSCs. We also assessed outcomes in 3 populations of interest (individuals receiving a health care subsidy [household income less than $39 250 and not eligible for Income Support], those receiving Income Support, and First Nations individuals) relative to the remainder of the population, controlling for whether care was provided in a PCN and adjusting for several baseline characteristics. Results We identified a total of 106 653 patients with diabetes eligible for our study, of whom 43 327 (41%) received care in a PCN. Receiving care through a PCN was associated with lower rates of ACSC-related hospital admission or emergency department visits for all groups of interest, which suggests that PCNs had similar effects across each group. However, regardless of where care was provided, First Nations and low-SES patients had more than twice the adjusted rates of hospital admission or emergency department visits for diabetes-specific ACSCs than the general population and were less likely to receive guideline-recommended care, including measurement of HbA1c and retinal screening. Interpretation Care in a PCN was associated with lower risks of hospital admission or emergency department visits for diabetes-specific ACSCs, even within vulnerable groups such as First Nations people and those of low SES. However, differences in outcomes and quality-of-care indicators persisted for First Nations individuals and those of low SES, relative to the general population, irrespective of where care was provided.
Stationary frame current regulation of PWM inverters with zero steady state error

Current regulators for AC inverters are commonly categorised as hysteresis, linear PI or deadbeat predictive, with a further subclassification into stationary ABC frame and synchronous DQ frame implementations. Synchronous frame controllers are generally accepted to have a better performance than stationary frame controllers do, as they operate on DC quantities and hence can eliminate steady state errors. This paper establishes a theoretical connection between these two classes of regulators and proposes a new type of stationary frame controller, which achieves the same steady state performance as a synchronous frame controller. The new controller is applicable to both single phase and three phase inverters.
Intra- and Extracellular Use and Evaluation of Ammonium-Selective Microelectrodes

Summary 1. An NH4 + -selective membrane for microelectrodes (NH4ISMs) was tested under 'biological conditions' in normal Astacus saline (NAS), two simulated intracellular salines (SIS) and the sensory neurone of the crayfish stretch receptor. 2. The effects of several physiological variables on intracellular NH 4 + measurements were tested in vitro. Changes in the background K + and Na + concentrations, the ratio K + /Na + , pH, ionic strength, osmotic pressure and volume were examined. 3. Phenomena specific for NH4ISMs, such as a positive potential shift, an undershoot and a difference between pre- and postcalibration curves are described and discussed. We propose to consider the values of intracellular NH 4 + concentration as apparent. 4. The detection limit of the NH4ISM is closely related to background K + concentration. It is in the region of O.lmmoU" 1
Magnetic field effects on neutrino production in microquasars

Aims. We investigate the effects of magnetic fields on neutrino production in microquasars. Methods. We calculated the steady particle distributions for the pions and muons generated in pγ and ppinteractions in the jet taking the effects of all energy losses into account. Results. The obtained neutrino emission is significantly modified due to the synchrotron losses suffered by secondary pions and muons. Conclusions. The estimates made for neutrino fluxes arriving on the Earth imply that detection of high-energy neutrinos from the vicinity of the compact object can be difficult. However, in the case of windy microquasars, the interaction of energetic protons in the jet with matter of dense clumps of the wind could produce detectable neutrinos. This is because the pions and muons at larger distances from the compact object will not be affected by synchrotron losses.
Simulation and performance estimation for the Rewrite Rule Machine

The authors give an overview of the Rewrite Rule Machine's (RRM's) architecture and discuss performance estimates based on very detailed register-level simulations at the chip level, together with more abstract simulations and modeling for higher levels. For a 10000 ensemble RRM, the present estimates are as follows. (1) The raw peak performance is 576 trillion operations per second. (2) For general symbolic applications, ensemble Sun-relative speedup is roughly 6.7, and RRM performance with a wormhole network at 88% efficiency gives an idealized Sun-relative speedup of 59000. (3) For highly regular symbolic applications (the sorting problem is taken as a typical example), ensemble performance is a Sun-relative speedup of 127, and RRM performance is estimated at over 80% efficiency (relative to the cluster performance), yielding a Sun-relative speedup of over 91. (4) For systolic applications (a 2-D fluid flow problem is taken as a typical example), ensemble performance is a Sun-relative speedup of 400-670, and cluster-level performance, which should be attainable in practice, is at 82% efficiency. <<ETX>>
Proteolytic Processing of ErbB4 in Breast Cancer

ErbB4 is a receptor tyrosine kinase that can signal by a mechanism involving proteolytic release of intracellular and extracellular receptor fragments. Proteolysis-dependent signaling of ErbB4 has been proposed to be enhanced in breast cancer, mainly based on immunohistochemical localization of intracellular epitopes in the nuclei. To more directly address the processing of ErbB4 in vivo, an ELISA was developed to quantify cleaved ErbB4 ectodomain from serum samples. Analysis of 238 breast cancer patients demonstrated elevated quantities of ErbB4 ectodomain in the serum (≥40 ng/mL) in 21% of the patients, as compared to 0% of 30 healthy controls (P = 0.002). Significantly, the elevated serum ectodomain concentration did not correlate with the presence of nuclear ErbB4 immunoreactivity in matched breast cancer tissue samples. However, elevated serum ectodomain concentration was associated with the premenopausal status at diagnosis (P = 0.04), and estradiol enhanced ErbB4 cleavage in vitro. A 3.4 Å X-ray crystal structure of a complex of ErbB4 ectodomain and the Fab fragment of anti-ErbB4 mAb 1479 localized the binding site of mAb 1479 on ErbB4 to a region on subdomain IV encompassing the residues necessary for ErbB4 cleavage. mAb 1479 also significantly blocked ErbB4 cleavage in breast cancer cell xenografts in vivo, and the inhibition of cleavage was associated with suppression of xenograft growth. These data indicate that ErbB4 processing is enhanced in breast cancer tissue in vivo, and that ErbB4 cleavage can be stimulated by estradiol and targeted with mAb 1479.
Baryons as Solitons in Three Dimensional Quantum Chromodynamics

We show that baryons of three-dimensional quantum chromodynamics can be understood as solitons of its effective Lagrangian. In the parity-preserving phase we study, these baryons are fermions for odd Nc and bosons for even Nc, never anyons. We quantize the collective variables of the solitons and thereby calculate the flavor quantum numbers. magnetic moments and mass splittings of the baryon. The flavor quantum numbers are in agreement with naive quark model for the low-lying states. The magnetic moments and mass splittings are smaller in the soliton model by a factor of logFπ/Ncmπ. We also show that there is a dibaryon solution that is an analog of the deuteron. These solitons can describe defects in a quantum antiferromagnet.
Bézier curve based trajectory planning for an intelligent wheelchair to pass a doorway

Door passing is the basic capability of an intelligent wheelchair. This paper presents a novel approach to address the door passing issue using Bézier curve based trajectory planning. The planed path consists of two segments: one from corridor to door and the other from door to the goal position. For each segment, an optimal Bézier curve is generated as a reference trajectory for an intelligent wheelchair to travel smoothly and accurately subject to corridor constraints, curvature limitation and obstacles. The simulation is conducted to verify the feasibility of the proposed approach, and the results show a good performance in terms of tracking accuracy and good maneuverability.
Memorable Tourism Experience (MTE): a scale proposal and test

This study sought to develop and test a scale to assess memorable tourism experiences (MTEs). The proposed instrument initially contained 49 tested items organised into 12 dimensions: environment, culture, relationships with companions, relationships with tourists, relationships with local agents (i.e. residents and service providers), novelty, emotions, dream, meaningfulness, refreshment, hedonism and involvement. The data were collected via a survey of 1,193 Brazilians with regular travel habits, aged 18 years or older. The data were analysed quantitatively using structural equation modelling. Statistical tests included exploratory factor analysis followed by confirmatory factor analysis. The results indicate that the scale is reliable and valid for research on MTEs, at least for the sample in question, and confirm the MTE construct’s multidimensionality, although the hedonism and involvement dimensions were shown to be unsuitable. Thus, the validity of the proposed MTE scale consisting of 35 items was confirmed, as were 10 dimensions of the second-order construct.
Resuscitation of Preterm Infants with Reduced Oxygen Results in Less Oxidative Stress than Resuscitation with 100% Oxygen

The objective of this study was to determine the effects of the level of inhaled oxygen during resuscitation on the levels of free radicals and anti-oxidative capacity in the heparinized venous blood of preterm infants. Forty four preterm infants <35 weeks of gestation with mild to moderate neonatal asphyxia were randomized into two groups. The first group of infants were resuscitated with 100% oxygen (100% O2 group), while in the other group (reduced O2 group), the oxygen concentration was titrated according to pulse oximeter readings. We measured total hydroperoxide (TH) and redox potential (RP) in the plasma within 60 min of birth. The integrated excessive oxygen (∑(FiO2-0.21) × Time(min)) was higher in the 100% O2 group than in the reduced O2 group (p<0.0001). TH was higher in the 100% O2 group than in the reduced O2 group (p<0.0001). RP was not different between the 100% O2 and reduced O2 groups (p = 0.399). RP/TH ratio was lower in the 100% O2 group than in the reduced O2 group (p<0.01). We conclude that in the resuscitation of preterm infants with mild to moderate asphyxia, oxidative stress can be reduced by lowering the inspired oxygen concentration using a pulse oximeter.
Development of an approximation scheme for quasi-exactly solvable double-well potentials

We make use of a recently developed method to, not only obtain the exactly known eigenstates and eigenvalues of a number of quasi-exactly solvable Hamiltonians, but also construct a convergent approximation scheme for locating those levels, not amenable to analytical treatments. The fact that, the above method yields an expansion of the wave functions in terms of corresponding energies, enables one to treat energy as a variational parameter, which can be effectively used for the identification of the eigenstates. It is particularly useful for the quasi-exactly solvable systems, where the ground state is known and a number of eigenstates are bounded, both below and above. The efficacy of the procedure is illustrated by obtaining, the low-lying excited states of a prototypical double-well potential, where the conventional techniques are not very reliable. Our approach yields the approximate eigenfunctions and eigenvalues, whose accuracy can be improved to any desired level, in a controlled manner. Comparing the present results with those of an independent numerical method, it was found that, the first few terms in our approximate solutions are enough to yield the excited state eigenvalues, accurate upto the third place of the decimal.
The throughput of hybrid-ARQ protocols for the Gaussian collision channel

In next-generation wireless communication systems, packet-oriented data transmission will be implemented in addition to standard mobile telephony. We take an information-theoretic view of some simple protocols for reliable packet communication based on "hybrid-ARQ," over a slotted multiple-access Gaussian channel with fading and study their throughput (total bit per second per hertz) and average delay under idealized but fairly general assumptions. As an application of the renewal-reward theorem, we obtain closed-form throughput formulas. Then, we consider asymptotic behaviors with respect to various system parameters. The throughput of automatic retransmission request (ARQ) protocols is compared to that of code division multiple access (CDMA) with conventional decoding. Interestingly, the ARQ systems are not interference-limited even if no multiuser detection or joint decoding is used, as opposed to conventional CDMA.
Multilevel Logistic Regression Analysis Applied to Binary Contraceptive Prevalence Data

In public health, demography and sociology, large-scale surveys often follow a hierarchical data structure as the surveys are based on multistage stratified cluster sampling. The appropriate approach to analyzing such survey data is therefore based on nested sources of variability which come from different levels of the hierarchy. When the variance of the residual errors is correlated between individual observations as a result of these nested structures, traditional logistic regression is inappropriate. We use the 2004 Bangladesh Demographic and Health Survey (BDHS) contraceptive binary data which is a multistage stratified cluster dataset. This dataset is used to exemplify all aspects of working with multilevel logistic regression models, including model conceptualization, model description, understanding of the structure of required multilevel data, estimation of the model via the statistical package MLwiN, comparison between different estimations, and investigation of the selected determinants of contraceptive use.
Perceptions of Principal Leadership on Teacher Morale

v Chapter 1: Introduction.1 Chapter 2: Review of Literature . 18 Chapter 3: Methodology . 32 Chapter 4: Presentation of the Findings.41 Chapter 5: Summary and Discussion.53 References.64 Appendix A: IRB Letter.72 Appendix B: Participant Consent Form . 73 Appendix C: Interview Questions . 75 Appendix D: Review of Transcript Letter . 76