Miracle-dz/UniFAR-FTUs huggingface dataset:
Mushroom Ganoderma lucidum Prevents Colitis-Associated Carcinogenesis in Mice

Background Epidemiological studies suggest that mushroom intake is inversely correlated with gastric, gastrointestinal and breast cancers. We have recently demonstrated anticancer and anti-inflammatory activity of triterpene extract isolated from mushroom Ganoderma lucidum (GLT). The aim of the present study was to evaluate whether GLT prevents colitis-associated carcinogenesis in mice. Methods/Principal Findings Colon carcinogenesis was induced by the food-borne carcinogen (2-Amino-1-methyl-6-phenylimidazol[4,5-b]pyridine [PhIP]) and inflammation (dextran sodium sulfate [DSS]) in mice. Mice were treated with 0, 100, 300 and 500 mg GLT/kg of body weight 3 times per week for 4 months. Cell proliferation, expression of cyclin D1 and COX-2 and macrophage infiltration was assessed by immunohistochemistry. The effect of GLT on XRE/AhR, PXR and rPXR was evaluated by the reporter gene assays. Expression of metabolizing enzymes CYP1A2, CYP3A1 and CYP3A4 in colon tissue was determined by immunohistochemistry. GLT treatment significantly suppressed focal hyperplasia, aberrant crypt foci (ACF) formation and tumor formation in mice exposed to PhIP/DSS. The anti-proliferative effects of GLT were further confirmed by the decreased staining with Ki-67 in colon tissues. PhIP/DSS-induced colon inflammation was demonstrated by the significant shortening of the large intestine and macrophage infiltrations, whereas GLT treatment prevented the shortening of colon lengths, and reduced infiltration of macrophages in colon tissue. GLT treatment also significantly down-regulated PhIP/DSS-dependent expression of cyclin D1, COX-2, CYP1A2 and CYP3A4 in colon tissue. Conclusions Our data suggest that GLT could be considered as an alternative dietary approach for the prevention of colitis-associated cancer.
Satellite and Numerical Model Investigation of Two Heavy Rain Events over the Central Mediterranean

AbstractTwo heavy rain events over the Central Mediterranean basin, which are markedly different by genesis, dimensions, duration, and intensity, are analyzed. Given the relative low frequency of this type of severe storms in the area, a synoptic analysis describing their development is included. A multispectral analysis based on geostationary multifrequency satellite images is applied to identify cloud type, hydrometeor phase, and cloud vertical extension. Precipitation intensity is retrieved from (i) surface rain gauges, (ii) satellite data, and (iii) numerical model simulations. The satellite precipitation retrieval algorithm 183-Water vapor Strong Lines (183-WSL) is used to retrieve rain rates and cloud hydrometeor type, classify stratiform and convective rainfall, and identify liquid water clouds and snow cover from the Advanced Microwave Sounding Unit-B (AMSU-B) sensor data. Rainfall intensity is also simulated with the Weather Research and Forecasting (WRF) numerical model over two nested domains w.
A comparison of filtered leukocyte-reduced and cytomegalovirus (CMV) seronegative blood products for the prevention of transfusion-associated CMV infection after marrow transplant.

We performed a prospective, randomized trial in CMV seronegative marrow recipients to determine if filtered blood products were as effective as CMV-seronegative blood products for the prevention of transfusion-transmitted CMV infection after marrow transplant. Before transplant, 502 patients were randomized to receive either filtered or seronegative blood products. Patients were monitored for the development of CMV infection and tissue-documented CMV disease between days 21 and 100 after transplant. Infections occurring after day 21 from transplant were considered related to the transfusion of study blood products and, thus, were considered evaluable infections for the purpose of this trial. In the primary analysis of evaluable infections, there were no significant differences between the probability of CMV infection (1.3% v 2.4%, P = 1.00) or disease (0% v 2.4%, P = 1.00) between the seronegative and filtered arms, respectively, or probability of survival (P = .6). In a secondary analysis of all infections occurring from day 0 to 100 post-transplant, although the infection rates were similar, the probability of CMV disease in the filtered arm was greater (2.4% v 0% in the seronegative arm, P = .03). However, the disease rate was still within the prestudy clinically defined acceptable rate of < or = 5%. We conclude that filtration is an effective alternative to the use of seronegative blood products for prevention of transfusion-associated CMV infection in marrow transplant patients.
Does concept-mapping strategy work for everyone? The levels of generativity and learners' self-regulated learning skills

The purpose of this study was to examine the effect of concept-mapping strategies with three different generativity levels (expert-generated concept map, partially learner-generated concept map, fully learner-generated concept map) on knowledge acquisition. Interaction between learners' self-regulated learning (SRL) skills and different levels of generativity was also tested. One hundred twenty-four undergraduate students, randomly assigned to three different concept-mapping groups, were differentiated by high and low levels of SRL skills. The findings suggest that the participants in the fully learner-generated map group significantly outperformed the participants in the expert-generated map group, and participants with high SRL skills significantly outperformed those with low SRL skills across all treatment levels. [ABSTRACT FROM AUTHOR]
Pregabalin Use Disorder.

Pregabalin is a gamma-aminobutyric acid (GABA) analog used for the treatment of partial epilepsy, neuropathic pain, fibromyalgia, and generalized anxiety disorder. As a GABA analog, there has been some concern about its abuse liability. The number of case reports on the abuse potential of pregabalin has increased (1,2,3,4). To our knowledge, this is the third case report of pregabalin use disorder in Turkey.
Nonsense-Mediated mRNA Decay and Loss-of-Function of the Protein Underlie the X-Linked Epilepsy Associated with the W356× Mutation in Synapsin I

Synapsins are a family of neuronal phosphoproteins associated with the cytosolic surface of synaptic vesicles. Experimental evidence suggests a role for synapsins in synaptic vesicle clustering and recycling at the presynaptic terminal, as well as in neuronal development and synaptogenesis. Synapsin knock-out (Syn1−/−) mice display an epileptic phenotype and mutations in the SYN1 gene have been identified in individuals affected by epilepsy and/or autism spectrum disorder. We investigated the impact of the c.1067G>A nonsense transition, the first mutation described in a family affected by X-linked syndromic epilepsy, on the expression and functional properties of the synapsin I protein. We found that the presence of a premature termination codon in the human SYN1 transcript renders it susceptible to nonsense-mediated mRNA decay (NMD). Given that the NMD efficiency is highly variable among individuals and cell types, we investigated also the effects of expression of the mutant protein and found that it is expressed at lower levels compared to wild-type synapsin I, forms perinuclear aggregates and is unable to reach presynaptic terminals in mature hippocampal neurons grown in culture. Taken together, these data indicate that in patients carrying the W356× mutation the function of synapsin I is markedly impaired, due to both the strongly decreased translation and the altered function of the NMD-escaped protein, and support the value of Syn1−/− mice as an experimental model mimicking the human pathology.
Research on disclosure of HIV status: cultural evolution finds an ally in science.

This editorial responds to Mason et al.'s (1995) "Culturally Sanctioned Secrets: Latino Men's Nondisclosure of HIV Infection to Family, Friends, and Lovers. " Culture is an evolving dynamic phenomenon shaped by society, psychology, and history. Historically, familism and simpatía have been Hispanic cultural assets. As times change, however, values and behaviors that served a culture for generations may become liabilities unless they evolve to fit the changing world of the culture. In the case of Hispanic gay men, the desire to protect family members is a barrier to disclosure of HIV status. Mason et al.'s study points to areas in which cultural development is needed. Science and culture thus become allies, with science pointing the way to needed directions for adaptive cultural evolution.
Ulysses at 50° south: constant immersion in the high‐speed solar wind

We present speed observations from the Ulysses solar wind plasma experiment through 50° south latitude. The pronounced speed modulation arising from solar rotation and the tilt of the heliomagnetic current sheet has nearly disappeared. Ulysses is now observing wind speeds in the 700 to 800 km s−1 range, with a magnetic polarity indicating an origin in the large south polar coronal hole. The strong compressions, rarefactions, and shock waves previously seen have weakened or disappeared. Occasional coronal mass ejections characterized by low plasma density caused by radial expansion have been observed. The coronal configuration was simple and stable in 1993, indicating that the observed solar wind changes were caused by increasing spacecraft latitude. Trends in prevailing speed with increasing latitude support previous findings. A decrease in peak speed southward of 40° latitude may indicate that the fastest solar wind comes from the equatorial extensions of the polar coronal holes.
Estradiol Inhibits Th17 Cell Differentiation through Inhibition of RORγT Transcription by Recruiting the ERα/REA Complex to Estrogen Response Elements of the RORγT Promoter

The symptoms of vaginal candidiasis exacerbate in the second half of the menstrual cycle in premenopausal women when the serum estradiol level is elevated. Estradiol has been shown to inhibit Th17 differentiation and production of antifungal IL-17 cytokines. However, little is known about the mechanisms. In the present study, we used mouse splenocytes and found that estradiol inhibited Th17 differentiation through downregulation of Rorγt mRNA and protein expression. Estradiol activated estrogen receptor (ER)α to recruit repressor of estrogen receptor activity (REA) and form the ERα/REA complex. This complex bound to three estrogen response element (ERE) half-sites on the Rorγt promoter region to suppress Rorγt expression. Estradiol induced Rea mRNA and protein expression in mouse splenocytes. Using Rea small interfering RNA to knock down Rea expression enhanced Rorγt expression and Th17 differentiation. Alternatively, histone deacetylase 1 and 2 bound to the three ERE half-sites, independent of estradiol. Histone deacetylase inhibitor MS-275 dose- and time-dependently increased Rorγt expression and subsequently enhanced Th17 differentiation. In 15 healthy premenopausal women, high serum estradiol levels are correlated with low RORγT mRNA levels and high REA mRNA levels in the vaginal lavage. These results demonstrate that estradiol upregulates REA expression and recruits REA via ERα to the EREs on the RORγT promoter region, thus inhibiting RORγT expression and Th17 differentiation. This study suggests that the estradiol/ERα/REA axis may be a feasible target in the management of recurrent vaginal candidiasis.
Neural aspects of ghrelin-induced gastroprotection against mucosal injury induced by noxious agents.

Ghrelin, identified in oxyntic mucosa has been recently implicated in the control of food intake and growth hormone (GH) release but whether this hormone can influence the gastric secretion and gastric mucosal integrity have been little studied. We compared the effects of intraperitoneal (i.p.) and intracerebroventricular (i.c.v.) administration of ghrelin on gastric secretion in rats equipped with gastric fistula (GF) and gastric lesions induced in rats by 75% ethanol and ischemia-reperfusion (I/R) with or without vagotomy or functional ablation of afferent sensory nerves by capsaicin. The number and the area of gastric lesions was measured by planimetry, the GBF was assessed by H(2)-gas clearance method and blood was withdrawn for the determination of the plasma ghrelin and gastrin levels. Ghrelin (5-80 microg/kg i.p. or 600-5000 ng/rat i.c.v.) increased gastric acid secretion and attenuated gastric lesions induced by ethanol and I/R. These protective effects of ghrelin were accompanied by the significant rise in the gastric mucosal blood flow (GBF) and plasma ghrelin and gastrin levels. Ghrelin given i.p. or injected i.c. v. in standard doses 20 microg/kg or 5000 ng/kg, respectively, significantly attenuated the gastric mucosal damage and significantly raised the GBF. Ethanol applied i.g. in smaller concentrations (12.5% and 25%) produced a significant increase in plasma immunorective ghrelin levels and this effect was inhibited in rats receiving ethanol in higher concentrations (75% and 100%). Ghrelin-induced protection after its i.p. or i.c. v. administration and accompanying increase in the GBF were completely abolished by vagotomy and capsaicin-deactivation of sensory nerves. Concurrent treatment with CGRP added to ghrelin restored the gastroprotective and hyperemic effects of ghrelin applied i.p. or i.c. v. in rats with capsaicin denervation. We conclude that central and peripheral ghrelin exerts a potent protective and gastric secretory effects in rats exposed to ethanol and I/R, and that these actions involve vagal nerve integrity, partially depending upon afferent nerves and hyperemia mediated by sensory neuropeptides such as CGRP released from these nerves.
CD44 Suppresses TLR-Mediated Inflammation1

The cell adhesion molecule CD44, which is the major hyaluronan receptor, has been implicated in the binding, endocytosis, and metabolism of hyaluronan. Previous studies have revealed that CD44 plays crucial roles in a variety of inflammatory diseases. In recent years, TLRs, which are ancient microbial pattern recognition receptors, have been shown to initiate an innate immune response and have been linked to a variety of inflammatory diseases. The present study shows that CD44 negatively regulates in vivo inflammation mediated by TLRs via NF-κB activation, which leads to proinflammatory cytokine production. Furthermore, our results show that CD44 directly associates with TLR2 when stimulated by the TLR2 ligand zymosan and that the cytoplasmic domain of CD44 is crucial for its regulatory effect on TLR signaling. This study indicates that CD44 plays a protective role in TLR-mediated inflammation and is the first to demonstrate a direct association between CD44 and a TLR.
The coefficient of earth pressure at rest

Laboratory experiments on undisturbed specimens of a large number of soft clay deposits, as well as previous measurements on clays and granular soils, were used to examine and explain the magnitude and behavior of the coefficient of earth pressure at rest, K0: (i) after sedimentation – primary consolidation, (ii) during secondary-compression aging, (iii) after active or passive preshearing away from the laterally constrained condition, (iv) during a decrease in effective vertical stress, and (v) during an increase in effective vertical stress in the recompression or compression range, in terms of , the slope of the effective horizontal versus effective vertical stress path. The behavior of K0 is explained using the concept of mobilized angle of friction in laterally constrained deformation condition. The Jaky equation provides, in terms of the angle of internal friction, a good estimate of K0 of sedimented, normally consolidated young clays and granular soils, as well as of of presheared clays and sands, .
Yesterday, my program worked. Today, it does not. Why?

Imagine some program and a number of changes. If none of these changes is applied (“yesterday”), the program works. If all changes are applied (“today”), the program does not work. Which change is responsible for the failure? We present an efficient algorithm that determines the minimal set of failure-inducing changes. Our delta debugging prototype tracked down a single failure-inducing change from 178,000 changed GDB lines within a few hours.
Impacts of Hydropower Development on Rural Livelihood Sustainability in Sikkim, India: Community Perceptions

Abstract Mountain economies are undergoing transformation from traditional agrarian to more industrial or service-oriented economies. Such changes invariably have socioeconomic impacts on nearby communities and lead to fragmentation and rural depopulation. Sikkim, a small state of India in the Eastern Himalayas, has recently embarked on a program of hydroelectricity project construction. This study examined community perceptions of the environmental and socioeconomic impacts of these projects in 3 rural areas and considers implications for future sustainable livelihoods. While benefits such as employment have accrued to the rural community from these economic development projects, changes in land use and in people's occupations may have adverse impacts on their future livelihoods. We argue that there is a need to support new types of land-based economic activities on abandoned agricultural lands, reclaim degraded lands, and introduce new products and production methods.
Expression of Cytokine Receptors by Human Cord Blood Lymphocytes: Comparison with Adult Blood Lymphocytes

ABSTRACT: The expression of receptors for several cytokines (IL 2, IL-4, IL-6, IL-7, tumor necrosis factor, and interferon-γ) was examined in human cord blood cells in comparison with adult blood cells. A previously described high sensitivity immunofluorescence procedure was used to render the low levels of receptor measurable. Cord blood lymphocytes expressed measurable levels of most cytokine receptors, but expression tended to be lower than in adult blood cells. Examination of different lymphocyte subpopulations revealed a complex pattern with some cell types expressing particular receptors equivalent to adult levels. Cord and adult blood monocytes expressed similar cytokine receptor profiles. Receptor expression in cord lymphocytes could be regulated by activation. The results provide indications as to the relative activities of different cytokines in the development of the immune system in the neonate.
Interaction of Mesoscale Variability with Large-Scale Waves in the Argentine Basin

Abstract The Argentine Basin of the South Atlantic Ocean is a region of complicated ocean dynamics involving a wide range of spatial and temporal scales. Previous studies reported the existence of a basin mode of topographic barotropic Rossby waves with a period close to 25 days in the region. Using observations of sea level anomalies from satellite altimeter measurements, the present study provides evidence of interaction between the large-scale 25-day waves and the energetic mesoscale variability of the region. The amplitude of the 25-day waves is highly intermittent with dominant periods in the range of 110–150 days. Within this period band, the wave amplitude is coherent with the energy level of the mesoscale variability: when the mesoscale energy level goes down, the wave amplitude goes up, and vice versa, suggesting an exchange of energy between the two scales. This coherence is linked to the first three empirical orthogonal functions (EOFs) of the sea level anomalies. The spatial patterns of these .
Aspirin-triggered 15-Epi-Lipoxin A4 (LXA4) and LXA4 Stable Analogues Are Potent Inhibitors of Acute Inflammation: Evidence for Anti-inflammatory Receptors

Lipoxins are bioactive eicosanoids that are immunomodulators. In human myeloid cells, lipoxin (LX) A4 actions are mediated by interaction with a G protein–coupled receptor. To explore functions of LXA4 and aspirin-triggered 5(S),6(R),15(R)-trihydroxy-7,9,13-trans-11-cis–eicosatetraenoic acid (15-epi-LXA4) in vivo, we cloned and characterized a mouse LXA4 receptor (LXA4R). When expressed in Chinese hamster ovary cells, the mouse LXA4R showed specific binding to [3H]LXA4 (K d ≈ 1.5 nM), and with LXA4 activated GTP hydrolysis. Mouse LXA4R mRNA was most abundant in neutrophils. In addition to LXA4 and 15-epi-LXA4, bioactive LX stable analogues competed with both [3H]LXA4 and [3H]leukotriene D4 (LTD4)– specific binding in vitro to neutrophils and endothelial cells, respectively. Topical application of LXA4 analogues and novel aspirin-triggered 15-epi-LXA4 stable analogues to mouse ears markedly inhibited neutrophil infiltration in vivo as assessed by both light microscopy and reduced myeloperoxidase activity in skin biopsies. The 15(R)-16-phenoxy-17,18, 19,20-tetranorLXA4 methyl ester (15-epi-16-phenoxy-LXA4), an analogue of aspirin triggered 15-epi-LXA4, and 15(S)-16-phenoxy-17,18,19,20-tetranor-LXA4 methyl ester (16-phenoxy-LXA4) were each as potent as equimolar applications of the anti-inflammatory, dexamethasone. Thus, we identified murine LXA4R, which is highly expressed on murine neutrophils, and showed that both LXA4 and 15-epi-LXA4 stable analogues inhibit neutrophil infiltration in the mouse ear model of inflammation. These findings provide direct in vivo evidence for an anti-inflammatory action for both aspirin-triggered LXA4 and LXA4 stable analogues and their site of action in vivo.
The IMF in a World of Private Capital Markets

The IMF attempts to stabilize private capital flows to emerging markets by providing public monitoring and emergency finance. In analyzing its role we contrast cases where banks and bondholders do the lending. Banks have a natural advantage in monitoring and creditor coordination, while bonds have superior risk sharing characteristics. Consistent with this assumption, banks reduce spreads as they obtain more information through repeat transactions with borrowers. By comparison, repeat borrowing has little influence in bond markets, where publicly-available information dominates. But spreads on bonds are lower when they are issued in conjunction with IMF-supported programs, as if the existence of a program conveyed positive information to bondholders. The influence of IMF monitoring in bond markets is especially pronounced for countries vulnerable to liquidity crises.
Spectral albedo of snow-covered first-year and multi-year sea ice during spring melt

Surface spectral-albedo data collected over snow-covered first-year and multi-year sea ice under diffuse sky conditions during the springtime transition are examined. Of specific interest is the relationship between changes in the visible and near-infrared albedo of sea ice and concurrent changes in the geophysical characteristics of the ice volume. With the onset of melt conditions, visible and near-infrared sea-ice albedo decreased due to physical changes within the snow and ice volumes. Visible albedo was found to be sensitive to changes occurring throughout the sea-ice volume, while the near-infrared albedo appeared most influenced by near-surface conditions.
Chemical and Storability of Fruit-Flavoured (Hibiscus sabdariffa) Drinks

2 Abstract: Roselle-fruit flavoured drinks were prepared from dried calyces of Hibiscus sabdariffa and fruits: apple, orange and pineapple. These were stored at three storage conditions: ambient, refrigeration and freezing. pH, titratable acidity and microbial quality of the samples were determined. pH decreased while titratable acidity increased with time for samples at ambient and refrigeration conditions. While microbial load of samples at ambient and refrigeration increased with time, those of samples at freezing condition decreased. Samples at ambient storage had a shelf-life of less of than five days while those at refrigeration condition stored for about a week and the samples at freezing condition could store for more than two weeks.