Miracle-dz/UniFAR-FTUs huggingface dataset:
The Use of Refuges by Communally Housed Cats

Simple Summary Captive domestic cats frequently suffer from the lack of physical space and opportunities to perform species-typical behaviors, such as climbing or hiding. Environmental enrichment is a technique that helps transform the space available to animals into a more appropriate habitat. In this study, we tested horizontal and vertical refuge boxes as environmental enrichment for cats living communally in a cat rescue shelter. The provision of boxes in the environment increases the use of available space by the cats. We suggest this improves the cats’ welfare while in communally-housed rescue shelters. Abstract The increase of domestic animals kept in shelters highlights the need to ensure animal welfare. Environmental enrichment can improve animal welfare in many ways, such as encouraging captive animals to use all the space available to them. The effects of physical environmental enrichment on the spatial distribution and behavioral repertoire of 35 neutered domestic cats housed communally were analyzed. The provision of boxes in the environment increases the use of available space by the cats. We suggest this improves the cats’ welfare while in communally-housed rescue shelters. The frequencies of active and especially inactive behaviors also increased in the enriched condition. In a test with vertical environmental enrichment, the animals showed an increased length of stay in refuges located at a height of 0.5 m compared to those on the ground (0.0 m). However, the entry frequency was higher in refuges at 0.0 m. Both horizontal and vertical environmental enrichment increased the use of available space, demonstrating that box refuges as enrichment are effective in providing a refuge when at a height, or a place to explore at ground level. We suggest it enhances the welfare of cats in communally housed shelters. This information adds to the body of evidence relating to cat enrichment and can be useful in designing cat housing in veterinary clinics, research laboratories, shelters and domestic homes.
NCAPG2 promotes tumour proliferation by regulating G2/M phase and associates with poor prognosis in lung adenocarcinoma

NCAPG2 is a component of the condensin II complex and contributes to chromosome segregation via microtubule–kinetochore attachment during mitosis. It is well known that NCAPG2 plays a critical role in cell mitosis; however, the role of altered NCAPG2 expression and its transcriptional regulatory function in cancer development remains mostly unknown. Here, for the first time we reported that NCAPG2 was evidently increased in non‐small cell lung cancer tissues compared to adjacent normal lung tissues. Clinicopathological data analysis showed that NCAPG2 overexpression was significantly correlated with lymph node metastasis and pathologic‐Tumour Nodes Metastasen stages, and was an independent prognostic factor in lung adenocarcinoma patients. Moreover, siRNA‐mediated knockdown of NCAPG2 could inhibit tumour cell growth of lung adenocarcinoma cells (A549 and H1299) in vitro and could significantly lead to cell cycle arrest in the G2 phase. Furthermore, we found that NCAPG2 silencing significantly decreased the expression levels of G2/M phase cell cycle‐related protein expressions (Cyclin B1, Cdc2) and increased the expression levels of p27 and p21 through Western blot analysis. Taken together, we demonstrated that increased NCAPG2 expression could regulate cell proliferation and identified as a poor prognostic biomarker in lung adenocarcinoma.
Mechanical and slurry abrasion behaviour of needle-punched nonwoven fabric reinforced epoxy composites

In this study, composites are fabricated using polyester fibre-based needle-punched nonwoven fabric mat as a reinforcement material, with different area density and at constant fabric loading. Experiments are conducted to study the effect of area density of fabric mat on mechanical and physical behaviour such as tensile strength, flexural strength, inter-laminar shear strength and density. Similarly, wear behaviour is also studied using slurry abrasion test rig by considering different parameters like sliding speed, normal load, sliding distance, silica sand size and area density. Taguchi orthogonal array is adopted to analyse the wear characteristics. Further, steady state results indicated that the wear rate of the composites decreased with the increase in normal load whereas increased with the increase in sliding velocity. The comparative analysis showed that the mechanical and physical properties of composites improved significantly with the increase in area density of the fabric.
The alcohol withdrawal syndrome

The alcohol withdrawal syndrome (AWS) is a common management problem in hospital practice for neurologists, psychiatrists and general physicians alike. Although some patients have mild symptoms and may even be managed in the outpatient setting, others have more severe symptoms or a history of adverse outcomes that requires close inpatient supervision and benzodiazepine therapy. Many patients with AWS have multiple management issues (withdrawal symptoms, delirium tremens, the Wernicke–Korsakoff syndrome, seizures, depression, polysubstance abuse, electrolyte disturbances and liver disease), which requires a coordinated, multidisciplinary approach. Although AWS may be complex, careful evaluation and available treatments should ensure safe detoxification for most patients.
Foot Drop Stimulation Versus Ankle Foot Orthosis After Stroke: 30-Week Outcomes

Background and Purpose— Drop foot after stroke may be addressed using an ankle foot orthosis (AFO) or a foot drop stimulator (FDS). The Functional Ambulation: Standard Treatment versus Electric Stimulation Therapy (FASTEST) trial was a multicenter, randomized, single-blinded trial comparing FDS and AFO for drop foot among people ≥3 months after stroke with gait speed ⩽0.8 m/s. Methods— Participants (n=197; 79 females and 118 males; 61.14±11.61 years of age; time after stroke 4.55±4.72 years) were randomized to 30 weeks of either FDS or a standard AFO. Eight dose-matched physical therapy sessions were provided to both groups during the first 6 weeks of the trial. Results— There was significant improvement within both groups from baseline to 30 weeks in comfortable gait speed (95% confidence interval for mean change, 0.11–0.17 m/s for FDS and 0.12–0.18 m/s for AFO) and fast gait speed. However, no significant differences in gait speed were found in the between-group comparisons. Secondary outcomes (standard measures of body structure and function, activity, and participation) improved significantly in both groups, whereas user satisfaction was significantly higher in the FDS group than in the control group. Conclusions— Using either an FDS or an AFO for 30 weeks yielded clinically and statistically significant improvements in gait speed and other functional outcomes. User satisfaction was higher in the FDS group. Although both groups did receive intervention, this large clinical trial provides evidence that FDS or AFO with initial physical therapy sessions can provide a significant and clinically meaningful benefit even years after stroke. Clinical Trial Registration Information— URL: http://www.clinicaltrials. gov. Unique Identifier: NCT01138995.
Intestinal expression of mutant and wild‐type progastrin significantly increases colon carcinogenesis in response to azoxymethane in transgenic mice

The authors recently reported that transgenic mice (hGAS) expressing pharmacologic levels of progastrin (PG) (> 10 nM to 100 nM) exhibited increased susceptibility to colon carcinogenesis in response to azoxymethane (AOM). It is not known whether PG functions as a cocarcinogen at the concentrations observed in patients with hypergastrinemia (∼1.0 nM).
Spectral line analysis by Pisarenko and Prony methods

A comparison of the Pisarenko and Prony approaches to spectral line analysis (sinusoid frequency and power estimation) is presented in this paper. The Pisarenko technique utilizes a statistical approach via the autocorrelation function. The Prony technique utilizes a deterministic approach via a least squares estimation procedure. The Prony method appears to have the edge on the Pisarenko technique. For one, the Prony method has fewer spurious components than does a Pisarenko line analysis. Also, a recursive procedure with lower computational complexity and a means to determine model order (number of sinusoidal components) is available with the Prony approach, a distinct advantage over the Pisarenko method.
Graph polynomials and approximation of partition functions with Loopy Belief Propagation

The Bethe approximation, or loopy belief propagation algorithm is a successful method for approximating partition functions of probabilistic models associated with a graph. Chertkov and Chernyak derived an interesting formula called Loop Series Expansion, which is an expansion of the partition function. The main term of the series is the Bethe approximation while other terms are labeled by subgraphs called generalized loops. In our recent paper, we derive the loop series expansion in form of a polynomial with coefficients positive integers, and extend the result to the expansion of marginals. In this paper, we give more clear derivation for the results and discuss the properties of the polynomial which is introduced in the paper.
A randomized, 4-week double-blind placebo control study on the efficacy of donepezil augmentation of lithium for treatment of acute mania

Introduction A significant number of mania patients fail to respond to current pharmacotherapy, thereby there is need for novel augmentation strategies. The results of some early studies showed the effectiveness of cholinomimetics in the treatment of mania. One open case series suggested the efficacy of donepezil in the treatment of bipolar disorder. Our aim was to explore whether an oral cholinesterase inhibitor, donepezil, administered during a 4-week treatment period, would benefit patients with acute mania. Methods We conducted a 4-week double-blind, placebo-controlled trial of donepezil as an adjunctive treatment to lithium in patients with acute mania. Eligible subjects were randomly assigned to receive donepezil or placebo in addition to lithium. Donepezil was started at 5 mg/day, and increased to 10 mg/day in the first week. Patients were rated with the Young Mania Rating Scale (YMRS) and Brief Psychiatric Rating Scale (BPRS) at baseline, day 1, week 1, week 2, and week 4. Results Out of the 30 patients who were enrolled, 15 were on donepezil and 15 were on placebo. All patients completed the 4-week trial. On the first day, there was a difference of 1.97 units on the psychomotor symptoms scale of the YMRS in the donepezil group as compared to the placebo group (t = 2.39, P = 0.02). There was a difference of 0.57 units (t = 2.09, P = 0.04) in the speech item and a difference of 0.29 units in the sexual interest item (t = 2.11, P = 0.04) in the donepezil group as compared to the placebo group. The total YMRS difference on the first day approached the conventional significance level (1.97 units, t = 1.84, P = 0.07). Over the course of 4 weeks, we failed to find that donepezil produced any significant difference in the YMRS (6.71 units difference, t = −1.44, P = 0.16) or the BPRS scale (1.29 units difference, t = −0.33, P = 0.75) as compared to placebo. Ten subjects (66.67%) in both groups met the criteria for clinical response (Fisher’s exact P = 1.00). Five subjects (33.33%) in the donepezil group met the criteria for clinical remission while nine subjects (60.00%) in the placebo group met the remission criteria (Fisher’s exact P = 0.27). Conclusion Use of the oral anticholinergic donepezil had some benefit in the augmentation of lithium treatment on the first day, but did not provide any significant benefits in the long-term.
Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects

Human cytomegalovirus (HCMV) infections of immunocompetent hosts are characterized by a dynamic, life-long interaction in which host immune responses, particularly of T cells, restrain viral replication and prevent disease but do not eliminate the virus or preclude transmission. Because HCMV is among the largest and most complex of known viruses, the T cell resources committed to maintaining this balance have never been characterized completely. Here, using cytokine flow cytometry and 13,687 overlapping 15mer peptides comprising 213 HCMV open reading frames (ORFs), we found that 151 HCMV ORFs were immunogenic for CD4+ and/or CD8+ T cells, and that ORF immunogenicity was influenced only modestly by ORF expression kinetics and function. We further documented that total HCMV-specific T cell responses in seropositive subjects were enormous, comprising on average ∼10% of both the CD4+ and CD8+ memory compartments in blood, whereas cross-reactive recognition of HCMV proteins in seronegative individuals was limited to CD8+ T cells and was rare. These data provide the first glimpse of the total human T cell response to a complex infectious agent and will provide insight into the rules governing immunodominance and cross-reactivity in complex viral infections of humans.
Simultaneous anterior-posterior approach to the thoracic and lumbar spine for the radical resection of tumors followed by reconstruction and stabilization.

OBJECT Thoracic or lumbar spine malignant tumors involving both the anterior and posterior columns represent a complex surgical problem. The authors review the results of treating patients with these lesions in whom surgery was performed via a simultaneous anterior-posterior approach. METHODS The hospital records of 26 patients who underwent surgery via simultaneous combined approach for thoracic and lumbar spinal tumors at our institution from July 1994 to March 2000 were reviewed. Surgery was performed with the patients in the lateral decubitus position for the procedure. The technical details are reported. The mean survival determined by Kaplan-Meier analysis was 43.4 months for the 15 patients with primary malignant tumors and 22.5 months for the 11 patients with metastatic spinal disease. At 1 month after surgery, 23 (96%) of 24 patients who complained of pain preoperatively reported improvements (p < 0.001, Wilcoxon signed-rank test), and eight (62%) of 13 patients with preoperative neurological deficits were functionally improved (p = 0.01). There were nine major complications, five minor complications, and no deaths within 30 days of surgery. Two patients (8%) later underwent surgery for recurrent tumor. CONCLUSIONS The simultaneous anterior-posterior approach is a safe and feasible alternative for the exposure tumors of the thoracic and lumbar spine that involve both the anterior and posterior columns. Advantages of the approach include direct visualization of adjacent neurovascular structures, the ability to achieve complete resection of lesions involving all three columns simultaneously (optimizing hemostasis), and the ability to perform excellent dorsal and ventral stabilization in one operative session.
Ocular manifestations in systemic lupus erythematosus

Systemic lupus erythematosus (SLE) can involve many parts of the eye, including the eyelid, ocular adnexa, sclera, cornea, uvea, retina and optic nerve. Ocular manifestations of SLE are common and may lead to permanent blindness from the underlying disease or therapeutic side effects. Keratoconjunctivitis sicca is the most common manifestation. However, vision loss may result from involvement of the retina, choroid and optic nerve. Ocular symptoms are correlated to systemic disease activity and can present as an initial manifestation of SLE. The established treatment includes prompt systemic corticosteroids, steroid-sparing immunosuppressive drugs and biological agents. Local ocular therapies are options with promising efficacy. The early recognition of disease and treatment provides reduction of visual morbidity and mortality.
Constitutive and Protein Kinase C‐Induced Internalization of the Dopamine Transporter is Mediated by a Clathrin‐Dependent Mechanism

The amount of dopamine transporter (DAT) present at the cell surface is rapidly regulated by the rates of DAT internalization to endosomes and DAT recycling back to the plasma membrane. The re‐distribution of the transporter from the cell surface to endosomes was induced by phorbol ester activation of protein kinase C in porcine aortic endothelial cells stably expressing the human DAT. Inhibition of DAT recycling with the carboxylic ionophore monensin also caused significant accumulation of DAT in early endosomes and a concomitant loss of DAT from the cell surface, due to protein kinase C‐independent constitutive internalization of DAT in the absence of recycling. Such monensin‐induced relocation of DAT to endosomes was therefore utilized as a measure of the constitutive internalization of DAT. Knock‐down of clathrin heavy chain or dynamin II by small interfering RNAs dramatically inhibited both constitutive and protein kinase C‐mediated internalization of DAT. In contrast, neither monensin‐dependent nor phorbol ester‐induced re‐distribution of DAT were affected by inhibitors of endocytosis through cholesterol‐rich membrane microdomains. Mutational analysis revealed the potential importance of amino acid residues 587–597 in DAT internalization. Altogether, the data suggest that both constitutive and protein kinase C‐mediated internalization of DAT utilize the clathrin‐dependent endocytic pathway, but likely involve unconventional mechanisms.
Custom-instruction synthesis for extensible-processor platforms

Efficiency and flexibility are critical, but often conflicting, design goals in embedded system design. The recent emergence of extensible processors promises a favorable tradeoff between efficiency and flexibility, while keeping design turnaround times short. Current extensible processor design flows automate several tedious tasks, but typically require designers to manually select the parts of the program that are to be implemented as custom instructions. In this work, we describe an automatic methodology to select custom instructions to augment an extensible processor, in order to maximize its efficiency for a given application program. We demonstrate that the number of custom instruction candidates grows rapidly with program size, leading to a large design space, and that the quality (speedup) of custom instructions varies significantly across this space, motivating the need for the proposed flow. Our methodology features cost functions to guide the custom instruction selection process, as well as static and dynamic pruning techniques to eliminate inferior parts of the design space from consideration. Furthermore, we employ a two-stage process, wherein a limited number of promising instruction candidates are first short-listed using efficient selection criteria, and then evaluated in more detail through cycle-accurate instruction set simulation and synthesis of the corresponding hardware, to identify the custom instruction combinations that result in the highest program speedup or maximize speedup under a given area constraint. We have evaluated the proposed techniques using a state-of-the-art extensible processor platform, in the context of a commercial design flow. Experiments with several benchmark programs indicate that custom processors synthesized using automatic custom instruction selection can result in large improvements in performance (up to 5.4/spl times/, an average of 3.4/spl times/), energy (up to 4.5/spl times/, an average of 3.2/spl times/), and energy-delay products (up to 24.2/spl times/, an average of 12.6/spl times/), while speeding up the design process significantly.
A role for sigma receptors in stimulant self-administration and addiction

Sigma-1 receptors (&sgr;1Rs) are structurally unique intracellular proteins that function as chaperones. &sgr;1Rs translocate from the mitochondria-associated membrane to other subcellular compartments, and can influence a host of targets, including ion channels, G-protein-coupled receptors, lipids, and other signaling proteins. Drugs binding to &sgr;Rs can induce or block the actions of &sgr;Rs. Studies indicate that stimulant self-administration induces the reinforcing effects of &sgr;R agonists, because of dopamine transporter actions. Once established, the reinforcing effects of &sgr;R agonists are independent of dopaminergic mechanisms traditionally thought to be critical to the reinforcing effects of stimulants. Self-administered doses of &sgr;R agonists do not increase dopamine concentrations in the nucleus accumbens shell, a transmitter and brain region considered important for the reinforcing effects of abused drugs. However, self-administration of &sgr;R agonists is blocked by &sgr;R antagonists. Several effects of stimulants have been blocked by &sgr;R antagonists, including the reinforcing effects, assessed by a place-conditioning procedure. However, the self-administration of stimulants is largely unaffected by &sgr;R antagonists, indicating fundamental differences in the mechanisms underlying these two procedures used to assess the reinforcing effects. When &sgr;R antagonists are administered in combination with dopamine uptake inhibitors, an effective and specific blockade of stimulant self-administration is obtained. Actions of stimulant drugs related to their abuse induce unique changes in &sgr;R activity and the changes induced potentially create redundant and, once established, independent reinforcement pathways. Concomitant targeting of both dopaminergic pathways and &sgr;R proteins produces a selective antagonism of stimulant self-administration, suggesting new avenues for combination chemotherapies to specifically combat stimulant abuse.
Multiple stressors threaten the imperiled coastal foundation species eelgrass (Zostera marina) in Chesapeake Bay, USA

Interactions among global change stressors and their effects at large scales are often proposed, but seldom evaluated. This situation is primarily due to lack of comprehensive, sufficiently long‐term, and spatially extensive datasets. Seagrasses, which provide nursery habitat, improve water quality, and constitute a globally important carbon sink, are among the most vulnerable habitats on the planet. Here, we unite 31 years of high‐resolution aerial monitoring and water quality data to elucidate the patterns and drivers of eelgrass (Zostera marina) abundance in Chesapeake Bay, USA, one of the largest and most valuable estuaries in the world, with an unparalleled history of regulatory efforts. We show that eelgrass area has declined 29% in total since 1991, with wide‐ranging and severe ecological and economic consequences. We go on to identify an interaction between decreasing water clarity and warming temperatures as the primary drivers of this trend. Declining clarity has gradually reduced eelgrass cover the past two decades, primarily in deeper beds where light is already limiting. In shallow beds, however, reduced visibility exacerbates the physiological stress of acute warming, leading to recent instances of decline approaching 80%. While degraded water quality has long been known to influence underwater grasses worldwide, we demonstrate a clear and rapidly emerging interaction with climate change. We highlight the urgent need to integrate a broader perspective into local water quality management, in the Chesapeake Bay and in the many other coastal systems facing similar stressors.