Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of multileaf collimator and conventional circular collimator systems in Cyberknife stereotactic radiotherapy

Abstract Multileaf collimator (MLC) technology has been newly introduced with the Cyberknife system. This study investigated the advantages of this system compared with the conventional circular collimator (CC) system. Dosimetric comparisons of MLC and CC plans were carried out. First, to investigate suitable target sizes for the MLC mode, MLC and CC plans were generated using computed tomography (CT) images from 5 patients for 1, 3, 5 and 7 cm diameter targets. Second, MLC and CC plans were compared in 10 patients, each with liver and prostate targets. For brain targets, doses to the brain could be spared in MLC plans better than in CC plans (P ≤ 0.02). The MLC mode also achieved more uniform dose delivery to the targets. The conformity index in MLC plans was stable, irrespective of the target size (P = 0.5). For patients with liver tumors, the MLC mode achieved higher target coverage than the CC mode (P = 0.04). For prostate tumors, doses to the rectum and the conformity index were lowered in MLC plans compared with in CC plans (P ≤ 0.04). In all target plans, treatment times in MLC plans were shorter than those in CC plans (P < 0.001). The newly introduced MLC technology can reduce treatment time and provide favorable or comparable dose distribution for 1–7 cm targets. In particular, the MLC mode has dosimetric advantage for targets near organs at risk. Therefore, the MLC mode is recommended as the first option in stereotactic body radiotherapy.
Experiments in Digital Literacy

Having digital literacy requires more than just the ability to use software or to operate a digital device; it includes a large variety of complex skills such as cognitive, motoric, sociological, and emotional that users need to have in order to use digital environments effectively. A conceptual model that was recently described by the authors suggests that digital literacy comprises five major digital skills: photo-visual skills ("reading" instructions from graphical displays), reproduction skills (utilizing digital reproduction to create new, meaningful materials from preexisting ones), branching skills (constructing knowledge from non-linear, hypertextual navigation), information skills (evaluating the quality and validity of information), and socio-emotional skills (understanding the "rules" that prevail in cyberspace and applying this understanding in online cyberspace communication). The present paper presents results from a performance-based pioneer study that investigated the application of the above digital literacy skills conceptual model among different groups of scholars. Results clearly indicate that the younger participants performed better than the older ones, with photo-visual and branching literacy tasks, whereas the older participants were found to be more literate in reproduction and information literacy tasks. Research results shed light on the cognitive skills that users utilize in performing with digital environments, and provide educators and software developers with helpful guidelines for designing better user-centered digital environments.
BRG1 expression is increased in human cutaneous melanoma

Summary Background The SWI/SNF chromatin remodelling complex plays important roles in cellular processes including cell differentiation, cell cycle control and DNA repair. Aberrant expression of SWI/SNF subunits is involved in cancer development. The core subunit of the SWI/SNF complex, SNF5, has been shown to be inactivated in malignant rhabdoid tumours and has been defined as a tumour suppressor. However, the role of the catalytic subunit, BRG1, is not well defined in cancer.
Self-efficacy as a moderator of the planning–behaviour relationship in interventions designed to promote physical activity

Individuals who lack perceived self-efficacy may benefit less from planning interventions because they fail to apply their plans when encountering situations that appear challenging. Thus, self-efficacy may operate as a moderator in relation to the effects of planning interventions on behaviour. This article reports from two experimental studies using cluster randomisation. Study 1 included 58 patients with diabetes who received a face-to-face action planning intervention to improve their physical activity levels. Study 2 targeted the roles of planning and self-efficacy for the maintenance of regular running and for relapse prevention in 187 active individuals over a 2-year period. The email-delivered intervention prompted specific goal setting and facilitated planning to mobilise social support to secure relapse prevention. Results of the two studies were consistent with a moderating effect of self-efficacy in relation to the planning–behaviour relationship. Individuals with high self-efficacy benefited from planning interventions. If a person lacks self-efficacy, planning may not effectively promote physical activity.
Silencing of APE1 Enhances Sensitivity of Human Hepatocellular Carcinoma Cells to Radiotherapy In Vitro and in a Xenograft Model

Resistance to radiotherapy is a key limitation for the treatment of human hepatocellular carcinoma (HCC). To overcome this problem, we investigated the correlation between radioresistance and the human apurinic/apyrimidinic endonuclease (APE1), a bifunctional protein, which plays an important role in DNA repair and redox regulation activity of transcription factors. In the present study, we examined the radiosensitivity profiles of three human HCC cell lines, HepG2, Hep3B, and MHCC97L, using the adenoviral vector Ad5/F35-mediated APE1 siRNA (Ad5/F35-siAPE1). The p53 mutant cell lines MHCC97L showed radioresistance, compared with HepG2 and Hep3B cells. APE1 was strongly expressed in MHCC97L cells and was induced by irradiation in a dose-dependent manner, and Ad5/F35-siAPE1 effectively inhibited irradiation-induced APE1 and p53 expression. Moreover, silencing of APE1 significantly potentiated the growth inhibition and apoptosis induction by irradiation in all tested human HCC cell lines. In addition, Ad5/F35-siAPE1 significantly enhanced inhibition of tumor growth and potentiated cell apoptosis by irradiation both in HepG2 and MHCC97L xenografts. In conclusion, down regulation of APE1 could enhance sensitivity of human HCC cells to radiotherapy in vitro and in vivo.
Examining pitch-accent variability from an exemplar-theoretic perspective

This paper presents an exemplar-theoretic account of pitch-accent variability. Results from two experiments are reported. The ﬁrst experiment examines variability / similarity across a range of pitch-accent contour types which vary in frequency of occurrence. The results report similar behaviour across the frequency bins and are indicative of pitch-accent immunity from frequency effects. The second experiment, in order to establish the impact of lexical frequency on pitch-accent production, examines the similarity of pitch-accent contours linked to high and low frequency syllables. The results indicate further autonomy on the part of pitch-accents and offer possible evidence for post-lexical pitch-accent generation.
Oral Health Status, Treatment Needs and Knowledge, Attitude and Practice of Health Care Workers of Ambala, India - A Cross-sectional Study

Background: Health care workers (HCWs) from an important component of the health care system of any nation. Adequate knowledge regarding oral health is also mandatory as it is directly related to general health. Aim: The present study was undertaken to assess oral health status and treatment needs of the health workers in Ambala district and to assess the knowledge, attitude and practices of HCWs. Subjects and Methods: A cross-sectional study was conducted among 148 HCWs of Ambala District. World Health Organization (WHO) Oral Health Performa-1997 was used to collect the data. For the diagnosis of dental caries, WHO type III examination was done using mouth mirrors and sharp probes while periodontal assessments were done by community periodontal index-probes. The data were analyzed using SPSS package, Chicago, IL, version 13.0. Results: Eating sweets and poor oral hygiene lead to dental caries were cited as the main reasons for dental caries by 62.2% (92/148) of subjects. Majority of the subjects (43.2%, 64/148) used to brush their teeth once a day. Mean number of decayed and missing teeth due to caries were 4.73 and 0.628 respectively. Prosthetic needs for maxillary arch were almost the same when compared to the prosthesis in the mandibular arch. Majority of the male (82.1%, 46/56) and female (79.3%, 73/92) subjects were having calculus. Conclusion: Attitude of the health workers toward oral health was poor as they had significantly higher treatment needs. The present study emphasized the need of regular dental checkup and health education of HCWs.
Multilocus Sequence Typing and Virulence Profiles in Uropathogenic Escherichia coli Isolated from Cats in the United States

The population structure, virulence, and antimicrobial resistance of uropathogenic E. coli (UPEC) from cats are rarely characterized. The aim of this study was to compare and characterize the UPEC isolated from cats in four geographic regions of USA in terms of their multilocus sequence typing (MLST), virulence profiles, clinical signs, antimicrobial resistance and phylogenetic grouping. The results showed that a total of 74 E. coli isolates were typed to 40 sequence types with 10 being novel. The most frequent phylogenetic group was B2 (n = 57). The most frequent sequence types were ST73 (n = 12) and ST83 (n = 6), ST73 was represented by four multidrug resistant (MDR) and eight non-multidrug resistant (SDR) isolates, and ST83 were significantly more likely to exhibit no drug resistant (NDR) isolates carrying the highest number of virulence genes. Additionally, MDR isolates were more diverse, and followed by SDR and NDR isolates in regards to the distribution of the STs. afa/draBC was the most prevalent among the 29 virulence-associated genes. Linking virulence profile and antimicrobial resistance, the majority of virulence-associated genes tested were more prevalent in NDR isolates, and followed by SDR and MDR isolates. Twenty (50%) MLST types in this study have previously been associated with human isolates, suggesting that these STs are potentially zoonotic. Our data enhanced the understanding of E. coli population structure and virulence association from cats. The diverse and various combinations of virulence-associated genes implied that the infection control may be challenging.
Atomistic understanding of kinetic pathways for single base-pair binding and unbinding in DNA

We combine free-energy calculations and molecular dynamics to elucidate a mechanism for DNA base-pair binding and unbinding in atomic detail. Specifically, transition-path sampling is used to overcome computational limitations associated with conventional techniques to harvest many trajectories for the flipping of a terminal cytosine in a 3-bp oligomer in explicit water. Comparison with free-energy projections obtained with umbrella sampling reveals four coordinates that separate true dynamic transition states from stable reactant and product states. Unbinding proceeds via two qualitatively different pathways: one in which the flipping base breaks its intramolecular hydrogen bonds before it unstacks and another in which it ruptures both sets of interactions simultaneously. Both on- and off-pathway intermediates are observed. The relation of the results to coarse-grained models for DNA-based biosensors is discussed.
Effects of feeding combinations of steam-flaked grain sorghum and steam-flaked, high-moisture, or dry-rolled corn on growth performance and carcass characteristics in feedlot cattle.

The objective of these two feeding trials was to determine the associative effects of feeding steam-flaked grain sorghum (SFGS) in combination with steam-flaked (SFC), dry-rolled (DRC), or high-moisture (HMC) corn on growth performance and carcass characteristics in feedlot cattle. In Trial 1, 200 yearling heifers were blocked by weight, allotted to 25 pens, and fed one of five finishing diets (77% grain, 15% corn silage, and 8% supplement on a DM basis) for an average of 137 d. The grain combinations were 100:0, 75:25, 50:50, 25:75, and 0:100 SFC:SFGS, respectively. Treatment had no effect on DMI (P > .05), but ADG, gain efficiency, and final live and hot carcass weights decreased linearly (P < .05) as the proportion of SFGS increased in the diet. Carcass backfat, quality grade, and liver abscess score were not affected (P > .05) by treatment. In Trial 2, 306 yearling steers were blocked by weight, allotted to 30 pens, and fed diets that contained 74.5% grain, 10% corn silage, 7.5% soybean meal, 4% tallow, and 4% supplement (DM basis) for an average of 139 d. The grain and grain combinations were 100% DRC, HMC, SFC, or SFGS and a 67%:33% combination of SFGS: DRC or SFGS:HMC. For steers fed diets containing a single source of grain, those fed SFC gained 7% more live weight and had a 7% higher gain efficiency (P < .05) than those fed DRC or HMC. Growth performance of steers fed SFGS was intermediate. Feeding grain combinations (67% SFGS:33% HMC or DRC) resulted in a 5 to 6% positive associative effect (P < .05) for ADG and gain efficiency. Carcass characteristics were not affected (P > .05) by treatment. We concluded that there were significant benefits (positive associative effects) when SFGS was fed in combination with DRC or HMC, but the effects were smaller when SFGS was fed in combination with SFC.
Acori graminei rhizoma Ameliorated Ibotenic Acid-Induced Amnesia in Rats

In the present study, we investigated the effects of Acori graminei rhizoma (AGR) on learning and memory for the Morris water maze task and on the central cholinergic system of the rats with excitotoxic medial septum (MS) lesion. On the water maze test, the rats were trained to find a platform that was in a fixed position during 6 days and then they received a 60 s probe trial in which the platform was removed from the pool on the 7th day. Ibotenic lesioning of the MS impaired the performance on the maze test and it caused degeneration of choline acetyltransferase and acetylcholine esterase in the hippocampus, which are markers of the central cholinergic system. Daily administrations of AGR (100 mg kg−1, i.p.) for 21 consecutive days produced reversals of the ibotenic acid-induced deficit in learning and memory. These treatments also reduced the loss of cholinergic immunoreactivity in the hippocampus that was induced by ibotenic acid. These results demonstrated that AGR ameliorated learning and memory deficits through their effects on the central nervous system, and neuroprotection was partly evaluated through the effect of AGR on the cholinergic system. Our studies suggest that AGR can possibly be used as treatment for Alzheimer's disease.
DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity

Zhang et al. show that DOCK8-deficient T and NK cells develop cell and nuclear shape abnormalities that do not impair chemotaxis but contribute to a form of cell death they term cytothripsis. Cytothripsis of DOCK8-deficient cells prevents the generation of long-lived skin-resident memory CD8 T cells resulting in impaired immune response to skin infection.
Alteration of molecular assembly of peroxiredoxins from hyperthermophilic archaea

Peroxiredoxin from Pyrococcus horikoshii (PhPrx) is a decameric protein formed by ring-type assembly of five dimers. To engineer the quaternary structure of PhPrx, we created a mutant PhPrx (PhPrx6m) by introducing six point mutations designed to dissociate PhPrx into dimers. Although PhPrx6m was a dimer in solution, the six dimers assembled into a dodecamer following crystallization. In the crystal structure, PhPrx6m was overoxidized, and the peroxidatic cysteine was in sulfonic acid form and two cysteines in the C-terminal region were linked by an intramolecular disulfide bond. Thus, we characterized the wild-type PhPrx overoxidized by hydrogen peroxide (PhPrxPer). Analytical ultracentrifugation showed that PhPrxPer had a higher molecular mass in solution than PhPrx. This was confirmed by analysis of the crystal structure of PhPrxPer, which was found to form a ring-type dodecamer composed of six dimers. The monomeric structures of PhPrx6m and PhPrxPer differed from that of PhPrx in the relative orientation of two domains, reflecting the number of dimers in the ring-type assembly. Unlike PhPrx, homologous peroxiredoxin from Aeropyrum pernix (ApPrx) did not undergo hexameric association. This property can be explained by the stronger connection between the two domains in ApPrx due to its C-terminal extension relative to PhPrx.
A Bayesian Method for Fitting Parametric and Nonparametric Models to Noisy Data

We present a simple paradigm for fitting models, parametric and nonparametric, to noisy data, which resolves some of the problems associated with classical MSE algorithms. This is done by considering each point on the model as a possible source for each data point. The paradigm can be used to solve problems which are ill-posed in the classical MSE approach, such as fitting a segment (as opposed to a line). It is shown to be nonbiased and to achieve excellent results for general curves, even in the presence of strong discontinuities. Results are shown for a number of fitting problems, including lines, circles, elliptic arcs, segments, rectangles, and general curves, contaminated by Gaussian and uniform noise.
Ejection Fraction and Mortality Rate of Patients with Isolated Acute Inferior Myocardial Infarction Reperfused by Streptokinase

BACKGROUND This study aimed to evaluate the effects of streptokinase on left ventricular ejection fraction and mortality rate of patients with inferior acute myocardial infarction (AMI) without right ventricular myocardial infarction (RVMI). METHODS Fifty five consecutive patients with the diagnosis of inferior AMI without RVMI in the coronary care unit (CCU) of Shariati Hospital in Isfahan were selected for this study. Patients who had a history and/or electrocardiogram (ECG) evidence of previous myocardial infarction, evidence of bundle branch block, historical or clinical findings of valvular or other non-coronary heart diseases or heart failure were excluded. Participants were divided into two groups. Group one (n=28) had no contraindication for taking thrombolytic therapy and group two (n=27) had at least one contraindication for this treatment. Patients in group one took 1,000,000 units streptokinase for one hour. Three days later, LVEF of all participants was measured by an experienced cardiologist using 2-dimentiona1 echocardiography. Patients were followed up until four weeks to assess the mortality rate. RESULTS One death in the first 24 hours was reported in group one. However, no death was reported in any group until four weeks after discharge. There was no significant difference in mortality rate during the first 24 hours and four weeks after discharge between the two groups. Mean LVEF in the two groups did not show any significant difference (P=0.21). CONCLUSION Probably streptokinase has no effects on one-month mortality rate and LVEF in patients with inferior AMI without RVMI. Therefore, streptokinase side effects must be taken into consideration when being administered for this group of patients.
QSTR with Extended Topochemical Atom Indices. 10. Modeling of Toxicity of Organic Chemicals to Humans Using Different Chemometric Tools

In this communication, we have developed quantitative predictive models using human lethal concentration values of 26 organic compounds including some pharmaceuticals with extended topochemical atom (ETA) indices applying different chemometric tools and compared the extended topochemical atom models with the models developed from non‐extended topochemical atom ones. Extended topochemical atom descriptors were also tried in combination with non‐extended topochemical atom descriptors to develop better predictive models. The use of extended topochemical atom descriptors along with non‐extended topochemical atom ones improved equation statistics and cross‐validation quality. The best model with sound statistical quality was developed from partial least squares regression using extended topochemical atom descriptors in combination non‐extended topochemical atom ones. Finally, to check true predictability of the ETA parameters, the data set was divided into training (n = 19) and test (n = 7) sets. Partial least squares and genetic partial least squares models were developed from the training set using extended topochemical atom indices and the models were validated using the test set. The extended topochemical atom models developed from different statistical tools suggest that the toxicity increases with bulk, chloro functionality, presence of electronegative atoms within a chain or ring and unsaturation, and decreases with hydroxy functionality and branching. The results suggest that the extended topochemical atom descriptors are sufficiently rich in chemical information to encode the structural features for QSAR/QSPR/QSTR modeling.
Transcription from the BamHI J fragment of herpes simplex virus type 1 (KOS)

RNA transfer experiments (Northern analyses) were used to localize polyadenylated mRNA species made after herpes simplex virus type 1 infection to EcoRI and BamHI fragments and subfragments from the short unique region of the herpes simplex virus type 1 (KOS) genome. Three predominant early mRNAs of 2.5, 1.3, and 0.9 kilobases map in the BamHI J fragment. A detailed restriction map of the BamHI J fragment was constructed.
Non-fragile state feedback H∞ control with quantized signals via LMI method

This paper presents a study on the problem of non-fragile state feedback H infin controller design for linear discrete-time systems with quantized signals. The quantizers considered here are dynamic and time-varying. With the consideration of controller gain variations and quantized signals at the same time, a new non-fragile H infin control strategy is proposed with updating quantizerpsilas parameters, such that the quantized closed-loop system is asymptotically stable and with a prescribed H infin performance bound. An example is presented to illustrate the effectiveness of the proposed control strategy.