Miracle-dz/UniFAR-FTUs huggingface dataset:
The Possible Role of Selenium Concentration in Hepatitis B and C Patients

Background/Aim: The compelling evidence reported that selenium is an essential trace mineral for human beings. Selenium plays a pivotal role in the restoration of immune functions. High rates of hepatitis B and C are present in Pakistan. Epidemiologic surveys demonstrated an inverse association between selenium (Se) level and regional cancer incidence, as well as viral infection. The present study was designed to evaluate the concentration of selenium in the serum of patients suffering from hepatitis B and C. Patients and Methods: In this cross-sectional descriptive analytical study, serum selenium concentration of 150 patients suffering from hepatitis B and C, along with 26 healthy controls, was determined by atomic absorption spectrophotometer equipped with hydride generation system, model Analytic Jena (Vario III). Results: The mean and standard deviation of serum selenium concentration observed in male and female patients with hepatitis C were 101.60±0.55 and 77.43±0.47 μ g/L, respectively, whereas the mean and standard deviation of serum selenium concentration observed in male and female patients with hepatitis B were 107.58±0.44 and 137.8±0.36 μg/L. Analysis of t test showed significant difference between C and B (P<0.001) patients in serum selenium concentration, when compared with the control. Conclusion: The obtained results indicate that serum selenium concentration of hepatitis B and C patients is less than serum selenium concentration of healthy individuals. However, serum selenium decline is relative to severity of disease. Based on findings of this study, it is proposed that selenium should be supplemented in such patients in order to optimize nutritional support and to get better treatment response.
A general cone-beam reconstruction algorithm

Considering the characteristics of the X-ray microscope system being developed at SUNY at Buffalo and the limitations of available cone-beam reconstruction algorithms, a general cone-beam reconstruction algorithm and several special versions of it are proposed and validated by simulation. The cone-beam algorithm allows various scanning loci, handles reconstruction of rod-shaped specimens which are common in practice, and facilitates near real-time reconstruction by providing the same computational efficiency and parallelism as L.A. Feldkamp et al.'s (1984) algorithm. Although the present cone-beam algorithm is not exact, it consistently gives satisfactory reconstructed images. Furthermore, it has several nice properties if the scanning locus meets some conditions. First, reconstruction within a midplane is exact using a planar scanning locus. Second, the vertical integral of a reconstructed image is equal to that of the actual image. Third, reconstruction is exact if an actual image is independent of rotation axis coordinate z. Also, the general algorithm can uniformize and reduce z-axis artifacts, if a helix-like scanning locus is used.
Vinculin-dependent Cadherin mechanosensing regulates efficient epithelial barrier formation

Summary Proper regulation of the formation and stabilization of epithelial cell–cell adhesion is crucial in embryonic morphogenesis and tissue repair processes. Defects in this process lead to organ malformation and defective epithelial barrier function. A combination of chemical and mechanical cues is used by cells to drive this process. We have investigated the role of the actomyosin cytoskeleton and its connection to cell–cell junction complexes in the formation of an epithelial barrier in MDCK cells. We find that the E-cadherin complex is sufficient to mediate a functional link between cell–cell contacts and the actomyosin cytoskeleton. This link involves the actin binding capacity of &agr;-catenin and the recruitment of the mechanosensitive protein Vinculin to tensile, punctate cell–cell junctions that connect to radial F-actin bundles, which we name Focal Adherens Junctions (FAJ). When cell–cell adhesions mature, these FAJs disappear and linear junctions are formed that do not contain Vinculin. The rapid phase of barrier establishment (as measured by Trans Epithelial Electrical Resistance (TER)) correlates with the presence of FAJs. Moreover, the rate of barrier establishment is delayed when actomyosin contraction is blocked or when Vinculin recruitment to the Cadherin complex is prevented. Enhanced presence of Vinculin increases the rate of barrier formation. We conclude that E-cadherin-based FAJs connect forming cell–cell adhesions to the contractile actomyosin cytoskeleton. These specialized junctions are sites of Cadherin mechanosensing, which, through the recruitment of Vinculin, is a driving force in epithelial barrier formation.
Logarithmic time cost optimal parallel sorting is not yet fast in practice

It is pointed out that, when looking for new and faster parallel sorting algorithms for use in massively parallel systems it is tempting to investigate promising alternatives from the large body of research done on parallel sorting in the field of theoretical computer science. It is shown how this kind of investigation can be done on a simple but versatile environment for programming and measuring of PRAM (parallel random access machine) algorithms. The practical value of Cole's parallel merge sort algorithm has been investigated by comparing it with Batcher's bitonic sorting. The O(log n) time consumption of Code's algorithm implies that it must be faster than bitonic sorting which is o(log/sup 2/ n) time, if n is large enough. However, it has been found that bitonic sorting is faster as long as n is less than 1.2*10/sup 21/. Consequently, it is concluded that Cole's logarithmic time algorithm is not fast in practice. <<ETX>>
Involvement of c‐Src tyrosine kinase in SHP‐1 phosphatase activation by Ang II AT2 receptors in rat fetal tissues

Angiotensin II (Ang II) AT2 receptors are abundantly expressed in rat fetal tissues where they probably contribute to development. In the present study we examine the effects of Ang II type 2 receptor stimulation on SHP‐1 activation. Ang II (10−7 M) elicits a rapid and transient tyrosine phosphorylation of SHP‐1, maximal at 1 min, in a dose‐dependent form, blocked by the AT2 antagonist, PD123319. SHP‐1 phosphorylation is followed in time by tyrosine dephosphorylation of different proteins, suggesting a sequence of events. Ang II induces association of SHP‐1 to AT2 receptors as shown by co‐immunoprecipitation, Western blot and binding assays. SHP‐1 activity was determined in immunocomplexes obtained with either anti‐AT2 or anti‐SHP‐1 antibodies, after Ang II stimulation (1 min), in correlation with the maximal level of SHP‐1 phosphorylation. Interestingly, following receptor stimulation (1 min) c‐Src was associated to AT2 or SHP‐1 immunocomplexes. Preincubation with the c‐Src inhibitor PP2 inhibited SHP‐1 activation and c‐Src association, thus confirming the participation of c‐Src in this pathway. We demonstrated here for the first time the involvement of c‐Src in SHP‐1 activation via AT2 receptors present in an ex vivo model expressing both receptor subtypes. In this model, AT2 receptors are not constitutively associated to SHP‐1 and SHP‐1 is not constitutively activated. Thus, we clearly establish that SHP‐1 activation, mediated by the AT2 subtype, involves c‐Src and precedes protein tyrosine dephosphorylation, in rat fetal membranes. J. Cell. Biochem. 105: 703–711, 2008. © 2008 Wiley‐Liss, Inc.
Evidence-based guidelines for the nutritional management of adult kidney transplant recipients.

OBJECTIVE The present article summarizes the key recommendations of the evidence-based guidelines developed for the nutritional management of adult kidney transplant recipients. BACKGROUND AND METHODS Nutrition interventions play an important role in preventing and managing common health problems associated with renal transplantation such as obesity, hypertension, diabetes, and cardiovascular disease. Two sets of guidelines were developed by a working group of renal dietitians and nephrologists. They were subject to expert panel review, and public consultation by renal clinicians and consumers before final endorsement by 2 authorities in Australia--Caring for Australasians with Renal Impairment (CARI) and Dietitians Association of Australia (DAA). Protocol and rigor of guideline development were previously described and published in the Journal of Renal Nutrition, 2009. RESULTS AND OUTCOMES These guidelines address 13 priority topics identified by the renal community and complement each other with different emphasis, from research translation to day to day clinical practice recommendations. The published guidelines are available to the public through web-access of CARI and DAA, and journal publications. Information includes the guidelines themselves with level of evidence stated, grading of recommendations, suggestions for clinical care, search strategy, background and summary of evidence, recommendations of other guidelines, practice recommendations, appendices of useful tools, and suggestions for audits and future research. CONCLUSIONS Two sets of comprehensive evidence-based nutrition guidelines from CARI and DAA are now available to help improve health outcomes of adult kidney transplant recipients.
Congenital toxoplasmosis. A prospective study of 378 pregnancies.

Of 378 pregnant women with high initial toxoplasma antibody titers or seroconversion during pregnancy, 183 acquired the infection during pregnancy, a rate of 6.3 per 100 pregnancies. There were 11 abortions; seven infants were stillborn or died. Toxoplasmosis occurred in 59 of the nonaborted offspring. Among these, two died, and seven had severe disease with cerebral and ocular involvement. Of the remaining 50, 11 had mild, and 39 had subclinical illness. Severe disease was noted only when maternal infections were acquired during the first two trimesters. Later acquisition resulted in subclinical or no fetal infections. Parasites were isolated from the placentas of 25 per cent of those who acquired toxoplasma during pregnancy. Treatment with spiramycin during pregnancy reduced the overall frequency of the fetal infections but not of overt disease. Mothers with antibodies before they became pregnant had no infected infants.
Spatial Distribution of Fibrosis Governs Fibrillation Wave Dynamics in the Posterior Left Atrium During Heart Failure

Heart failure (HF) commonly results in atrial fibrillation (AF) and fibrosis, but how the distribution of fibrosis impacts AF dynamics has not been studied. HF was induced in sheep by ventricular tachypacing (220 bpm, 6 to 7 weeks). Optical mapping (Di-4-ANEPPS, 300 frames/sec) of the posterior left atrial (PLA) endocardium was performed during sustained AF (burst pacing) in Langendorff-perfused HF (n=7, 4 &mgr;mol/L acetylcholine; n=3, no acetylcholine) and control (n=6) hearts. PLA breakthroughs were the most frequent activation pattern in both groups (72.0±4.6 and 90.2±2.7%, HF and control, respectively). However, unlike control, HF breakthroughs preferentially occurred at the PLAs periphery near the pulmonary vein ostia, and their beat-to-beat variability was greater than control (1.93±0.14 versus 1.47±0.07 changes/[beats/sec], respectively, P<0.05). On histological analysis (picrosirius red), the area of diffuse fibrosis was larger in HF (23.4±0.4%) than control (14.1±0.6%; P<0.001, n=4). Also the number and size of fibrous patches were significantly larger and their location was more peripheral in HF than control. Computer simulations using 2-dimensional human atrial models with structural and ionic remodeling as in HF demonstrated that changes in AF activation frequency and dynamics were controlled by the interaction of electrical waves with clusters of fibrotic patches of various sizes and individual pulmonary vein ostia. During AF in failing hearts, heterogeneous spatial distribution of fibrosis at the PLA governs AF dynamics and fractionation.
On DOA estimation in unknown colored noise-fields using an imperfect estimate of the noise covariance

Most algorithms for direction-of-arrival (DOA) estimation require the noise covariance matrix to be known or to possess a known structure. In many cases the noise covariance is in fact estimated from separate measurements. This paper addresses the combined effects of finite sample sizes both in the estimated noise covariance matrix and in the data with signals present. It is assumed that a batch of signal-free samples is available in addition to the signal-containing samples. No assumption is made on the structure of the noise covariance. In this work the asymptotical covariance of weighted subspace fitting (WSF) is derived for the case when the data are whitened using an estimated noise covariance. The obtained expression suggests an optimal weighting that improves performance compared to the standard choice. In addition, a new method based on covariance matching is proposed. The proposed method is by construction asymptotically efficient. Monte Carlo simulations show promising small sample performance for the two new methods and confirm the asymptotical results. The CRB for the data model is included in the numerical evaluations and it is found to coincide with the WSF asymptotical covariance derived
Computational Analysis of Viscoelastic Properties of Crosslinked Actin Networks

Mechanical force plays an important role in the physiology of eukaryotic cells whose dominant structural constituent is the actin cytoskeleton composed mainly of actin and actin crosslinking proteins (ACPs). Thus, knowledge of rheological properties of actin networks is crucial for understanding the mechanics and processes of cells. We used Brownian dynamics simulations to study the viscoelasticity of crosslinked actin networks. Two methods were employed, bulk rheology and segment-tracking rheology, where the former measures the stress in response to an applied shear strain, and the latter analyzes thermal fluctuations of individual actin segments of the network. It was demonstrated that the storage shear modulus (G′) increases more by the addition of ACPs that form orthogonal crosslinks than by those that form parallel bundles. In networks with orthogonal crosslinks, as crosslink density increases, the power law exponent of G′ as a function of the oscillation frequency decreases from 0.75, which reflects the transverse thermal motion of actin filaments, to near zero at low frequency. Under increasing prestrain, the network becomes more elastic, and three regimes of behavior are observed, each dominated by different mechanisms: bending of actin filaments, bending of ACPs, and at the highest prestrain tested (55%), stretching of actin filaments and ACPs. In the last case, only a small portion of actin filaments connected via highly stressed ACPs support the strain. We thus introduce the concept of a ‘supportive framework,’ as a subset of the full network, which is responsible for high elasticity. Notably, entropic effects due to thermal fluctuations appear to be important only at relatively low prestrains and when the average crosslinking distance is comparable to or greater than the persistence length of the filament. Taken together, our results suggest that viscoelasticity of the actin network is attributable to different mechanisms depending on the amount of prestrain.
Tunable and laser-reconfigurable 2D heterocrystals obtained by epitaxial stacking of crystallographically incommensurate Bi2Se3 and MoS2 atomic layers

Heterocrystals: rotationally oriented stacks of incommensurate 2D materials with tunable and laser-reconfigurable properties. Vertical stacking is widely viewed as a promising approach for designing advanced functionalities using two-dimensional (2D) materials. Combining crystallographically commensurate materials in these 2D stacks has been shown to result in rich new electronic structure, magnetotransport, and optical properties. In this context, vertical stacks of crystallographically incommensurate 2D materials with well-defined crystallographic order are a counterintuitive concept and, hence, fundamentally intriguing. We show that crystallographically dissimilar and incommensurate atomically thin MoS2 and Bi2Se3 layers can form rotationally aligned stacks with long-range crystallographic order. Our first-principles theoretical modeling predicts heterocrystal electronic band structures, which are quite distinct from those of the parent crystals, characterized with an indirect bandgap. Experiments reveal striking optical changes when Bi2Se3 is stacked layer by layer on monolayer MoS2, including 100% photoluminescence (PL) suppression, tunable transmittance edge (1.1→0.75 eV), suppressed Raman, and wide-band evolution of spectral transmittance. Disrupting the interface using a focused laser results in a marked the reversal of PL, Raman, and transmittance, demonstrating for the first time that in situ manipulation of interfaces can enable “reconfigurable” 2D materials. We demonstrate submicrometer resolution, “laser-drawing” and “bit-writing,” and novel laser-induced broadband light emission in these heterocrystal sheets.
Antioxidant Effects of Brown Algae Sargassum on Sperm Parameters

AbstractThe occurrence of oxidative stress during the sperm freeze-thaw cycles affects the sperm parameters and eventually leads to a decrease in its reproductive potential. Sperm protection against oxidative reactions during freezing is done by antioxidants. Since the selection of a suitable sperm cryopreservation bank is effective in maintaining acceptable reproductive potential and motility of sperm during cryopreservation.This study aimed to evaluate the antioxidant effects of different doses of the extract of brown algae Sargassum on oxidative stress and frozen human sperm parameters.We conducted a randomized controlled trial on the semen samples from 11 healthy men in the age group of 25 to 36 years. The samples were collected by masturbation after 3 to 5 days of abstinence from ejaculation. The specimens were divided into 3 equal parts, including 1 control group and 2 experimental groups.The 2 experimental groups were frozen using the rapid solidification technique with Sargassum extract at doses of 250 and 500 &mgr;g/mL.Motility and morphology of sperms were measured using a computer system and CASA software and the amount of reactive oxygen species was determined using Oxisperm kit.Sargassum extract significantly decreased the amount of reactive oxygen species (P < 0.005) and at doses of 250 and 500 &mgr;g/mL, significantly increased the overall motility (P < 0.006) and progressive motility (P < 0.007) after solidification, but did not affect the normal morphology of sperms.The addition of ethanol extract of Sargassum prevents reactive oxygen species production during the solidification process and improves sperm motility at doses of 250 and 500 &mgr;g/mL.
The effectiveness of low laser therapy in subacromial impingement syndrome: a randomized placebo controlled double‐blind prospective study

OBJECTIVES: Conflicting results were reported about the effectiveness of Low level laser therapy on musculoskeletal disorders. The aim of this study was to investigate the effectiveness of 850‐nm gallium arsenide aluminum (Ga‐As‐Al) laser therapy on pain, range of motion and disability in subacromial impingement syndrome. METHODS: A total of 52 patients (33 females and 19 males with a mean age of 53.59±11.34 years) with subacromial impingement syndrome were included. The patients were randomly assigned into two groups. Group I (n = 30, laser group) received laser therapy (5 joule/cm2 at each point over maximum 5‐6 painful points for 1 minute). Group II (n = 22, placebo laser group) received placebo laser therapy. Initially cold pack (10 minutes) was applied to all of the patients. Also patients were given an exercise program including range of motion, stretching and progressive resistive exercises. The therapy program was applied 5 times a week for 14 sessions. Pain severity was assessed by using visual analogue scale. Range of motion was measured by goniometer. Disability was evaluated by using Shoulder Pain and Disability Index. RESULTS: In group I, statistically significant improvements in pain severity, range of motion except internal and external rotation and SPADI scores were observed compared to baseline scores after the therapy (p<0.05). In Group II, all parameters except range of motion of external rotation were improved (p<0.05). However, no significant differences were recorded between the groups (p>0.05). CONCLUSIONS: The Low level laser therapy seems to have no superiority over placebo laser therapy in reducing pain severity, range of motion and functional disability.
Prediction of Methotrexate Clinical Response in Portuguese Rheumatoid Arthritis Patients: Implication of MTHFR rs1801133 and ATIC rs4673993 Polymorphisms

Objective. Methotrexate (MTX), the most used drug in rheumatoid arthritis (RA) treatment, showing variability in clinical response, is often associated with genetic polymorphisms. This study aimed to elucidate the role of methylenetetrahydrofolate reductase (MTHFR) C677T and aminoimidazole carboxamide adenosine ribonucleotide transformylase (ATIC) T675C polymorphisms and clinicopathological variables in clinical response to MTX in Portuguese RA patients. Methods. Study included 233 RA patients treated with MTX for at least six months. MTHFR C677T and ATIC T675C polymorphisms were genotyped and clinicopathological variables were collected. Statistical analyses were performed and binary logistic regression method adjusted to possible confounding variables. Results. Multivariate analyses demonstrated that MTHFR 677TT (OR = 4.63; P = 0.013) and ATIC 675T carriers (OR = 5.16; P = 0.013) were associated with over 4-fold increased risk for nonresponse. For clinicopathological variables, noncurrent smokers (OR = 7.98; P = 0.001), patients positive to anti-cyclic citrullinated peptide (OR = 3.53; P = 0.004) and antinuclear antibodies (OR = 2.28; P = 0.045), with higher health assessment questionnaire score (OR = 2.42; P = 0.007), and nonsteroidal anti-inflammatory drug users (OR = 2.77; P = 0.018) were also associated with nonresponse. Contrarily, subcutaneous administration route (OR = 0.11; P < 0.001) was associated with response. Conclusion. Our study suggests that MTHFR C677T and ATIC T675C genotyping combined with clinicopathological data may help to identify patients whom will not benefit from MTX treatment and, therefore, assist clinicians in personalizing RA treatment.
Range Nearest-Neighbor Query

A range nearest-neighbor (RNN) query retrieves the nearest neighbor (NN) for every point in a range. It is a natural generalization of point and continuous nearest-neighbor queries and has many applications. In this paper, we consider the ranges as (hyper)rectangles and propose efficient in-memory processing and secondary memory pruning techniques for RNN queries in both 2D and high-dimensional spaces. These techniques are generalized for kRNN queries, which return the k nearest neighbors for every point in the range. In addition, we devise an auxiliary solution-based index EXO-tree to speed up any type of NN query. EXO-tree is orthogonal to any existing NN processing algorithm and, thus, can be transparently integrated. An extensive empirical study was conducted to evaluate the CPU and I/O performance of these techniques, and the study showed that they are efficient and robust under various data sets, query ranges, numbers of nearest neighbors, dimensions, and cache sizes.