Miracle-dz/UniFAR-FTUs huggingface dataset:
Maintenance of age in human neurons generated by microRNA-based neuronal conversion of fibroblasts

Aging is a major risk factor in many forms of late-onset neurodegenerative disorders. The ability to recapitulate age-related characteristics of human neurons in culture will offer unprecedented opportunities to study the biological processes underlying neuronal aging. Here, we show that using a recently demonstrated microRNA-based cellular reprogramming approach, human fibroblasts from postnatal to near centenarian donors can be efficiently converted into neurons that maintain multiple age-associated signatures. Application of an epigenetic biomarker of aging (referred to as epigenetic clock) to DNA methylation data revealed that the epigenetic ages of fibroblasts were highly correlated with corresponding age estimates of reprogrammed neurons. Transcriptome and microRNA profiles reveal genes differentially expressed between young and old neurons. Further analyses of oxidative stress, DNA damage and telomere length exhibit the retention of age-associated cellular properties in converted neurons from corresponding fibroblasts. Our results collectively demonstrate the maintenance of age after neuronal conversion. DOI: http://dx.doi. org/10.7554/eLife.18648. 001
Why are they grumbling about my new system? Theoretical foundation and empirical evidence of employee grumbling as a user resistance behavior

This research theorizes employee grumbling as a user resistance phenomenon observed during early information system (IS) implementation phases. When the usage of new IS is mandatory, user resistance cannot be observed when focusing technology usage, instead, employees protest against the IS implementation, or spoke rather negatively of it. This form of user resistance behavior is conceptualized by the newly proposed variable employee grumbling, which provides a different perspective on user resistance that can be used especially in early implementation phases to observe resistance behaviors. Perceived ease of use and usefulness, affective and cognitive resistance to change, and individual differences and basic tendencies are analyzed as antecedents based on study with 106 employees during an IS implementation. The results of the analysis reveal that especially affective resistance to change determine employee grumbling, and technology perceptions are less important in early implementation.
A Novel Selenocystine-Accumulating Plant in Selenium-Mine Drainage Area in Enshi, China

Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.
Temperature and Mortality in Nine US Cities

Background: Extreme temperatures have been associated with increased mortality worldwide. The extent to which air pollutants may confound or modify this association remains unclear. Methods: We examined the association between mean apparent temperature and total mortality in 9 cities across the United States during the warm season (May to September) from 1999 to 2002. We applied case-crossover and time-series analyses, adjusting for day of the week and season in time-series analysis. City-specific estimates were then combined using a meta-analysis. A total of 213,438 deaths for all causes occurred in these cities during the study period. Results: We found that mortality increased with apparent temperature. A 5.5°C (10°F) increase in apparent temperature was associated with an increase in mortality of 1.8% (95% confidence interval = 1.09% to 2.5%) when using case-crossover analysis and with an increase of 2.7% (2.0% to 3.5%) using the time-series analysis. Conclusions: This study provides evidence of increased mortality due to elevated apparent temperature exposure, with no confounding or effect modification due to air pollution.
Do Women Have an Empathic Accuracy Advantage? Empathic Accuracy – Hybrid Offspring of Interpersonal Accuracy and Empathy Concentrate Only on a Narrow Segment of Interpersonal Accuracy, Looking Exclusively at Accuracy Motivation Made for a Woman

Our local newspaper recently printed a cartoon in which one teenage female character asked her young adult brother why he's glum and he responds ―It's nuthin'. ‖ In the next frame, with only his laconic answer to go on, she summarizes his current career, social and romantic woes. In the final frame, he asks ―How do you DO that? ‖ to which she responds with a shrug, ―I'm female. ‖ The funny pages and aphorisms are full of stereotypes about women's ―intuition‖ and what seems at times, perhaps particularly to men, women's uncanny ability to read others' thoughts. This chapter asks whether this reputation is deserved, and if so, wherein lies the source of women's skill. Greater aptitude? Greater motivation? Or some combination of the two? Two paradigm for measuring empathic accuracy–the ability to accurately infer other people's thoughts and feelings – has provided quite a bit of empirical data that address this question. The results of this research, however, have not been generous in providing simple answers. A rich mix of motivation and gender role expectations, combined with a messy set of outcomes that defy simple clarity, must be considered to understand women's ―sometimes‖ advantage at empathic accuracy. No wonder ―women's intuition‖ has a gauzy mystique to it. When we pull back the veil on empathic accuracy, the female advantage is considerably less impressive than the stereotypes would have us believe. And yet, as it turns out, there is something to the myth. After reviewing the literature linking women and empathic accuracy in order to write this chapter, our conclusions, which (spoiler alert! ) we will give away up front and later elaborate upon, have been whittled down to three qualified statements: 1. Questioning or challenging a person's interpersonal sensitivity motivates greater empathic accuracy from women, but not from men, which sets this particular manipulation apart from other incentives that seem more gender-neutral. 2. Communion, the habitual motivation to attend to and make connections with others that is a central feature of the feminine gender role, is predictive of empathic accuracy—but only some of the time. That lukewarm endorsement gets a slight boost when one notes that communion is one of few individual difference variables whose ability to predict empathic accuracy has been replicated. On the other hand, as often as not, there is no discernible effect of communion—and even when there is, it is very small. 3. Even in the absence of …
A measurement study of zigbee-based indoor localization systems under RF interference

With an expected market value of 2.71 billion in 2016, supporting daily use of real-time location systems in households and commercial buildings is an increasingly important subject of study. A growing problem in providing robust indoor location estimations in real time is the use of wireless transmissions in RF frequencies. Having implemented a simple RSSI-signature-based location system on a 24-node IEEE 802.15. 4-based sensor network testbed, we are able to analyze the effect of background IEEE 802.11 traffic on localization error. The measurement results demonstrate that the 80th-percentile of the localization error may increase by 141% at worst in an office building with active use of IEEE 802.11 for data. Such performance degradation results from RSSI reading loss as the beacon messages collide with background traffic.
Structural Effect of Different EDC Crosslinker Concentration in Gelatin-Hyaluronic Acid Scaffolds

Introduction: Gelatin and hyaluronic acid are two biopolymers with different applications in tissue engineering. They may be employed to construct diverse scaffolds that allow cells to differentiate and proliferate on them. In order to obtain the best functional and mechanical conditions in scaffolds, they must be crosslinked to form covalent links between gelatin and hyaluronic acid. The crosslinker 1-ethy-3-(3-dymethylaminopropyl) carbodiimide hydrochloride (EDC) is a compound widely used due to its low cytotoxicity. Besides, the concentration of the crosslinker may modify the physical properties and morphological characteristics of scaffolds when it forms covalent links between biopolymers, helping to construct different kinds of scaffolds used for developing soft tissues. However, the development of scaffolds made of gelatin and hyaluronic acid crosslinked with EDC has been poorly studied. In addition, the concentrations used for crosslinking gelatin and hyaluronic acid are contradictory. Therefore, the aim of this study was to analyze the structure of gelatin/hyaluronic acid scaffolds crosslinked with EDC. Methods: Gelatin-hyaluronic acid scaffolds were prepared by direct freeze-drying. Afterwards, They were crosslinked with different concentrations of EDC (6, 30, 50 and 60 mM) for 12 h. Results: This research has demonstrated that the gelatin/hyaluronic acid scaffolds crosslinked with the highest concentrations of the crosslinker had fewer water concentration absorbed, pore size diminished and pore number increased in comparison with control groups. Despite scaffolds composition has not changed in any of the concentrations, the bone marrow mesenchymal cells mortality percentage increased when cells were placed on the scaffolds of concentration 60 mM, perhaps for the residual 1-ethy-3-(3-dymethylaminopropyl) carbodiimide hydrochloride found in the scaffolds. Conclusion: Our results revealed that different EDC concentrations may modify the physical and biological characteristics of gelatin/hyaluronic acid scaffolds; as a result, the scaffolds obtained may be used for the manufacture of different tissues in regenerative medicine.
Impact of the Siewert Classification on the Outcome of Patients Treated by Preoperative Chemoradiotherapy for a Nonmetastatic Adenocarcinoma of the Oesophagogastric Junction

The aim of the study is to analyze the impact of the Siewert classification on the pathological complete response (pcR), pattern of failure, and general outcome of patients treated, by preoperative chemoradiotherapy and surgery for an gastroesophageal junction adenocarcinoma (OGJA). From 2000 to 2008, the charts of 68 patients were retrospectively reviewed. Tumor staging reported was UST1/T2/T3/T4/unknown, respectively, n = 1/7/54/5/1 patients, and N0/N1/unknown, respectively, n = 9/58/1 patients. Patients received primary external-beam radiotherapy with concurrent chemotherapy followed by surgical resection (Siewert I: upper oesogastrectomy; Siewert II/III: total gastrectomy with lower oesophagectomy). Overall survival (OS), overall relapse rate (ORR), cumulative rate of local (CRLR), nodal (CRNR), and metastatic (CRMR) relapse, and their prognostic factors were retrospectively analyzed. Median follow-up was 77.5 months. Median OS was 41.7 ± 5.2 months. The 3-year ORR was 48%. Using univariate analysis ORR was significantly increased for patients with Siewert II/III compared to Siewert I tumors (27.3% versus 62%, p = 0.047). Siewert I tumors had also statistically lower CRNR and CRMR compared to Siewert II/III tumors (0/9.1% versus 41.3/60.2% resp., p = 0.012), despite an equivalent cumulative rate of local relapse and pathological complete response rate between the three groups. For OGJA treated with preoperative CRT and surgery, ORR and CRMR were lower for patients with Siewert I tumors in comparison with Siewert II/III tumors.
Synaptic Output of Individual Layer 4 Neurons in Guinea Pig Visual Cortex

More than 90% of geniculocortical axons from the dorsal lateral geniculate nucleus of the thalamus innervate layer 4 (L4) of V1 (primary visual cortex). Excitatory neurons, which comprise >80% of the neuronal population in L4, synapse mainly onto adjacent L4 neurons and layer 2/3 (L2/3) neurons. It has been suggested that intralaminar L4–L4 connections contribute to amplifying and refining thalamocortical signals before routing to L2/3. To unambiguously probe the properties of the synaptic outputs from these L4 excitatory neurons, we used multiple simultaneous whole-cell patch-clamp recording and stimulation from two to four neighboring L4 neurons. We recorded uEPSCs (evoked unitary synaptic currents) in response to pairs of action potentials elicited in single presynaptic L4 neurons for 102 L4 cell pairs and found that their properties are more diverse than previously described. Averaged unitary synaptic response peak amplitudes spanned almost three orders of magnitude, from 0.42 to 192.92 pA. Although connections were, on average, reliable (average failure rate, 25%), we recorded a previously unknown subset of unreliable (failure rates from 30 to 100%) and weak (averaged response amplitudes, <5 pA) connections. Reliable connections with high probability of neurotransmitter release responded to paired presynaptic pulses with depression, whereas unreliable connections underwent paired-pulse facilitation. Recordings from interconnected sets of L4 triplets revealed that synaptic response amplitudes and reliability were equally variable between independent cell pairs and those that shared a common presynaptic or postsynaptic cell, suggesting local perisynaptic influences on the development and/or state of synaptic function.
On 2 + 2-Dimensional Spacetimes, Strings, Black-Holes and Maximal Acceleration in Phase Spaces

We study black-hole-like solutions ( spacetimes with singularities ) of Einstein field equations in 3+1 and 2+2-dimensions. In the 3+1-dim case, it is shown how the horizon of the standard black hole solution at r = 2GNM can be displaced to the location r = 0 of the point mass M source, when the radial gauge function is chosen to have an ultra-violet cutoff R(r = 0) = 2GNM if, and only if, one embeds the problem in the Finsler geometry of the spacetime tangent bundle (or in phase space) that is the proper arena where to incorporate the role of the physical point mass M source at r = 0. We find three different cases associated with hyperbolic homogeneous spaces. In particular, the hyperbolic version of Schwarzschild's solution contains a conical singularity at r = 0 resulting from pinching to zero size r = 0 the throat of the hyperboloid H2 and which is quite different from the static spherically symmetric 3+1-dim solution. Static circular symmetric solutions for metrics in 2+2 are found that are singular at ρ = 0 and whose asymptotic ρ → ∞ limit leads to a flat 1+2-dim boundary of topology S1 x R2. Finally we discuss the 1+1-dim Bars-Witten stringy black-hole solution and show how it can be embedded into our 3 + 1-dimensional solutions with a displaced horizon at r = 0 and discuss the plausible stringy nature of a point-mass, along with the maximal acceleration principle in the spacetime tangent bundle (maximal force in phase spaces). Black holes in a 2 + 2-dimensional "spacetime" from the perspective of complex gravity in 1 + 1 complex dimensions and their quaternionic and octonionic gravity extensions deserve furher investigation. An appendix is included with the most general Schwarzschild-like solutions in D ≥ 4.
Understanding social capital in sports clubs: participation, duration and social trust

Abstract A central claim in Robert Putnam’s theory of social capital is that participation in voluntary organizations can foster social trust among members that are active and engaged. Based on this claim, this article examines how duration of membership and member participation impacts upon levels of social trust among sports club members. The results show that neither participation nor duration impacts on levels of social trust – a result found in survey studies conducted in Denmark and the Netherlands. Sophisticated participation measures and the inclusion of duration of membership set this study apart from previous studies on social capital. The fact that no correlation can be identified between participation and duration on one side and social trust on the other adds weight to the claim that, even though numerous studies find higher levels of social trust among members of voluntary organizations than among non-members, this cannot be attributed to member socialization.
Effects of Varied Animation Strategies in Facilitating Animated Instruction

Background The use of animation and audio as a virtual panacea for everything from advertising to educational videos and instruction has created the presumption that any materials that use them ‘must be better! ’ Now doubt that the addition of animation can improve message delivery on a number of scales, but the use of animation regardless of message and with little concern for systematic placement may be causing more harm than good. Combined with the increasing prevalence of computing technology and increasing ease of development on standard workstations the integration of animation in web-based instruction is a more realistic possibility. This study explores the effect of animation on higher order educational objective achievement in a web-based, selfpaced programmed instructional unit on the human heart and its functions for undergraduate students with majors outside of the life sciences.
Mentor-protégé expectation agreement, met expectations, and perceived effort

PERCEIVED EFFORT: AN EXAMINATION OF THE EFFECTS ON KEY OUTCOMES FOR MENTORING RELATIONSHIPS by ANGIE LYNN LOCKWOOD (Under the Direction of Lillian T. Eby) ABSTRACT To date, research on mentoring relationships has focused on developing a normative model, outlining key antecedents, processes, and outcomes. In line with Leader-Member Exchange theory, this study examined the unique, idiosyncratic nature of mentoring relationships using data from 106 matched formal mentoring dyads. Contrary to predictions, met expectations did not mediate the relationship between mentor-protégé agreement on expectations for protégé/mentor behaviors and affective outcomes. Instead, perceived effort was the key driver of met expectations and demonstrated direct and indirect effects on relationship quality, protégés’ willingness to mentor, and protégés’ perception of program effectiveness. Additionally, mentorprotégé agreement on protégé role modeling and friendship behaviors was associated with mentors’ affective outcomes. Implications of non-hypothesized findings and suggestions for future research are discussed.
Exploring Gender Differences in SMS-Based Mobile Library Search System Adoption

This paper investigates differences in how male and female students perceived a short message service (SMS) library catalog search service when adopting it. Based on a sample of 90 students, the results suggest that there are significant differences in perceived usefulness and intention to use but no significant differences in selfefficacy and perceived ease of use between genders. The findings reveal that SMS efficiency has a significant influence on self-efficacy for males but not for females. Online Public Access Catalog (OPAC) experience has a stronger but less significant influence on self-efficacy for females but very little influence for males. Perceived usefulness is still the driving force behind intention to use for both genders. The findings suggest that factors influencing self-efficacy and its impact on Technology Acceptance Model (TAM) constructs are different for each gender. The implications of these findings are discussed for both researchers and practitioners.
Galaxy halo occupation at high redshift

We discuss how current and future data on the clustering and number density of Lyman-break galaxies (LBGs) can be used to constrain their relationship to dark matter haloes. We explore a three-parameter model in which the number of LBGs per dark halo scales like a power law in the halo mass: for . Here, Mmin is the minimum mass halo that can host an LBG, M1 is a normalization parameter, associated with the mass above which haloes host more than one observed LBG, and S determines the strength of the mass-dependence. We show how these three parameters are constrained by three observable properties of LBGs: the number density, the large-scale bias and the fraction of objects in close pairs. Given these three quantities, the three unknown model parameters may be estimated analytically, allowing a full exploration of parameter space. As an example, we assume a ΛCDM cosmology and consider the observed properties of a recent sample of spectroscopically confirmed LBGs. We find that the favoured range for our model parameters is , , and . The preferred region in Mmin expands by an order of magnitude, and slightly shallower slopes are acceptable if the allowed range of bg is permitted to span all recent observational estimates. We also discuss how the observed clustering of LBGs as a function of luminosity can be used to constrain halo occupation, although because of current observational uncertainties we are unable to reach any strong conclusions. Our methods and results can be used to constrain more realistic models that aim to derive the occupation function N(M) from first principles, and offer insight into how basic physical properties affect the observed properties of LBGs.
Kesantunan Berbahasa Ditinjau dari Prespektif Kecerdasan Majemuk

Politeness is not solely concerned the language proficiency but a form of multiple intelligence. In the reality of life is not a high IQ who became the only determinants ofsuccess alive. Many people who are cognitively smart but failed in life. On the contrary, many people who are cognitively average but a success in life. Theoretically, there are other factors which are thought to be the deciding one's success in life, among others, the ability to speak in a polite form of intelligence is linguistic, interpersonal, intercultural and personal. Because politeness is a form of intelligence, hence his acquisition should be through education, both in the institution of formal and non-formal. Children need to be educated to use polite language as the embodiment ofa good personal identity, in order to succeed in wading through life. This paper discusses the relationship between politeness and intelligence compounds. Some theory of politeness language (linguistic politeness theory) try combined with compound theory of intelligence (KM), which was conceived by Howard Gardner (1983). Gardner finds evidence that linguistic intelligence is one form of intelligence compound. In the perspective of theory of language, the Gardner's view can be realized in the form of politeness language. The theory is the theory that the new reatif in the field of psychology, relevant as Basic politeness in speaking. Key words: politeness, multiple intelligence, speech .