Miracle-dz/UniFAR-FTUs huggingface dataset:
Low Concentration of Quercetin Antagonizes the Cytotoxic Effects of Anti-Neoplastic Drugs in Ovarian Cancer

Objective The role of Quercetin in ovarian cancer treatment remains controversial, and the mechanism is unknown. The aim of this study was to investigate the therapeutic effects of Quercetin in combination with Cisplatin and other anti-neoplastic drugs in ovarian cancer cells both in vitro and in vivo, along with the molecular mechanism of action. Methods Quercetin treatment at various concentrations was examined in combination with Cisplatin, taxol, Pirarubicin and 5-Fu in human epithelial ovarian cancer C13* and SKOV3 cells. CCK8 assay and Annexin V assay were for cell viability and apoptosis analysis, immunofluorescence assay, DCFDA staining and realtime PCR were used for reactive oxygen species (ROS)-induced injury detection and endogenous antioxidant enzymes expression. Athymic BALB/c-nu nude mice were injected with C13*cells to obtain a xenograft model for in vivo studies. Immunohistochemical analysis was carried out to evaluate the ROS-induced injury and SOD1 activity of xenograft tumors. Results Contrary to the pro-apoptotic effect of high concentration (40 µM–100 µM) of Quercetin, low concentrations (5 µM–30 µM) of Quercetin resulted in varying degrees of attenuation of cytotoxicity of Cisplatin treatment when combined with Cisplatin. Similar anti-apoptotic effects were observed when Quercetin was combined with other anti-neoplastic agents: Taxol, Pirarubicin and 5-Fluorouracil (5-Fu). Low concentrations of Quercetin were observed to suppress ROS-induced injury, reduce intracellular ROS level and increase the expression of endogenous antioxidant enzymes, suggesting a ROS-mediated mechanism of attenuating anti-neoplastic drugs. In xenogeneic model, Quercetin led to a substantial reduction of therapeutic efficacy of Cisplatin along with enhancing the endogenous antioxidant enzyme expression and reducing ROS-induced damage in xenograft tumor tissue. Conclusion Taken together, these data suggest that Quercetin at low concentrations attenuate the therapeutic effects of Cisplatin and other anti-neoplastic drugs in ovarian cancer cells by reducing ROS damage. Quercetin supplementation during ovarian cancer treatment may detrimentally affect therapeutic response.
Discrepancy Between Mental Rotation and Perspective-Taking Abilities in Normal Aging Assessed by Piaget's Three-Mountain Task

In a cross-sectional study, we examined age-related differences in visuo-spatial ability associated with image rotation, using two variants of Piaget's ‘Three-Mountain Task. ’ The object-mental rotation (OMR) task detects the ability to mentally rotate an image, whereas the subject-mental rotation (SMR) task reveals the ability to mentally change one's perspective. A group of 33 young adults, 26 middle-aged adults, and 31 elderly normal adults were studied. Both tasks revealed age-related differences in performance but a larger difference between middle-aged and elderly group was observed for SMR than OMR performance. Age-related increases in the ‘egocentric’ type of error were found only on the SMR task. The results suggest that the ability to mentally change one's perspective declines with age, perhaps more than the ability to mentally rotate objects.
An Application Programme for Examination of Differential Linear and Nonlinear Magnification Methods and Magnification Interface Factors

The variable visual acuity nature of human vision across fovea and periphery prompted the need to study the differential magnification method as compared with the traditional linear magnification. With differential magnification, outer-area objects were magnified at a level higher than the inner-area objects. An application software was specially developed by the authors previously for the examination of differential linear and traditional linear magnification methods. This paper presents the enhancement of the application software and the addition of other magnification interface factors which worth further investigating. The newly designed differential nonlinear magnification method was featured and comparisons were made with the differential linear and the traditionally linear magnification methods.
In vitro genotoxicity of airborne Ni‐NP in air–liquid interface

Studies using advanced toxicological methods enabling in vitro conditions that are more realistic are currently needed for understanding the risks of pulmonary exposure to airborne nanoparticles. Owing to the carcinogenicity of certain nickel compounds, the increased production of nickel nanoparticles (Ni‐NPs) raises occupational safety concerns. The aim of this study was to investigate the genotoxicity of airborne Ni‐NPs using a recently developed air–liquid interface exposure system. The wild‐type Chinese hamster lung fibroblast cell line (V79) was used and cytotoxicity, DNA damage and mutagenicity were studied by testing colony forming efficiency, alkaline DNA unwinding and HPRT mutation assays, respectively. Additionally, co‐exposure to a PARP‐1 inhibitor was performed to test possible involvement of base excision repair (BER) in repair of Ni‐induced DNA damage. The results showed that cell viability was reduced significantly (to 45% and 46%) after 48 hours Ni‐NP exposure at concentrations of 0.15 and 0.32 μg cm−2. DNA damage was significantly increased after Ni‐NP exposure in the presence of the BER inhibitor indicating that Ni‐NP‐induced DNA damages are subsequently repaired by BER. Furthermore, there was no increased HPRT mutation frequency following Ni‐NP exposure. In conclusion, this study shows that Ni‐NP treatment of lung fibroblasts in an air–liquid interface system that mimics real‐life exposure, results in increased DNA strand breaks and reduced cellular viability. These DNA lesions were repaired with BER in an error‐free manner without resulting in mutations. This study also underlines the importance of appropriate quantification of the actual exposure concentrations during air–liquid interface exposure studies.
Biocompatibility of orthodontic adhesives in rat subcutaneous tissue

Objective The objective of the present study was to verify the hypothesis that no difference in biocompatibility exists between different orthodontic adhesives. Material and Methods Thirty male Wistar rats were used in this study and divided into five groups (n=6): Group 1 (control, distilled water), Group 2 (Concise), Group 3 (Xeno III), Group 4 (Transbond XT), and Group 5 (Transbond plus Self-Etching Primer). Two cavities were performed in the subcutaneous dorsum of each animal to place a polyvinyl sponge soaked with 2 drops of the respective adhesive in each surgical loci. Two animals of each group were sacrificed after 7, 15, and 30 days, and their tissues were analyzed by using an optical microscope. Results At day 7, Groups 3 (Transbond XT) and 4 (Xeno III) showed intense mono- and polymorphonuclear inflammatory infiltrate with no differences between them, whereas Groups 1 (control) and 2 (Concise) showed moderate mononuclear inflammatory infiltrate. At day 15, severe inflammation was observed in Group 3 (Transbond XT) compared to other groups. At day 30, the same group showed a more expressive mononuclear inflammatory infiltrate compared to other groups. Conclusion Among the orthodontic adhesive analyzed, it may be concluded that Transbond XT exhibited the worst biocompatibility. However, one cannot interpret the specificity of the data generated in vivo animal models as a human response.
Illustrating microorganisms: Sir William Watson Cheyne (1852–1932) and bacteriology

Sir William Watson Cheyne is largely known to medical history as Lord Lister’s ‘trusted assistant’. 1 He spent a lifetime defending Joseph Lister’s (1827–1912) antiseptic principle in the wake of scepticism and misunderstanding. However, his main contribution to Lister’s work was in the embryonic field of bacteriology in the 1870s–1890s, which brought him into contact with continental researchers, particularly Robert Koch (1843–1910). In this field, Cheyne built an independent reputation as an assessor, chronicler and promoter of continental laboratory methodology. He pioneered bacteriological training in British teaching hospitals and incorporated laboratory testing into case notes as standard procedure. This paper reconsiders Cheyne’s contribution to the development of bacteriology in British medicine at the end of the 19th century. It examines his motives in promoting new laboratory techniques and the methods he used to embed them in hospital procedure. It also considers how he continued to use bacteriological arguments to keep the Listerian antiseptic principle on the medical agenda well after Lister withdrew from active involvement in the field.
Molecular Cloning and Characterization of Tetrahydroprotoberberine cis-N-Methyltransferase, an Enzyme Involved in Alkaloid Biosynthesis in Opium Poppy*

S-Adenosyl-l-methionine:tetrahydroprotoberberine cis-N-methyltransferase (EC 2.1.1.122) catalyzes the conversion of (S)-stylopine to the quaternary ammonium alkaloid, (S)-cis-N-methylstylopine, as a key step in the biosynthesis of protopine and benzophenanthridine alkaloids in plants. A full-length cDNA encoding a protein exhibiting 45 and 48% amino acid identity with coclaurine N-methyltransferase from Papaver somniferum (opium poppy) and Coptis japonica, respectively, was identified in an elicitor-treated opium poppy cell culture expressed sequence tag data base. Phylogenetic analysis showed that the protein belongs to a unique clade of enzymes that includes coclaurine N-methyltransferase, the predicated translation products of the Arabidopsis thaliana genes, At4g33110 and At4g33120, and bacterial S-adenosyl-l-methionine-dependent cyclopropane fatty acid synthases. Expression of the cDNA in Escherichia coli produced a recombinant enzyme able to convert the protoberberine alkaloids stylopine, canadine, and tetrahydropalmatine to their corresponding N-methylated derivatives. However, the protoberberine alkaloids tetrahydroxyberbine and scoulerine, and simple isoquinoline, benzylisoquinoline, and pavine alkaloids were not accepted as substrates, demonstrating the strict specificity of the enzyme. The apparent Km values for (R,S)-stylopine and S-adenosyl-l-methionine were 0.6 and 11.5 μm, respectively. TNMT gene transcripts and enzyme activity were detected in opium poppy seedlings and all mature plant organs and were induced in cultured opium poppy cells after treatment with a fungal elicitor. The enzyme was detected in cell cultures of other members of the Papaveraceae but not in species of related plant families that do not accumulate protopine and benzophenanthridine alkaloids.
Fixed-dimensional acoustic embeddings of variable-length segments in low-resource settings

Measures of acoustic similarity between words or other units are critical for segmental exemplar-based acoustic models, spoken term discovery, and query-by-example search. Dynamic time warping (DTW) alignment cost has been the most commonly used measure, but it has well-known inadequacies. Some recently proposed alternatives require large amounts of training data. In the interest of finding more efficient, accurate, and low-resource alternatives, we consider the problem of embedding speech segments of arbitrary length into fixed-dimensional spaces in which simple distances (such as cosine or Euclidean) serve as a proxy for linguistically meaningful (phonetic, lexical, etc.) dissimilarities. Such embeddings would enable efficient audio indexing and permit application of standard distance learning techniques to segmental acoustic modeling. In this paper, we explore several supervised and unsupervised approaches to this problem and evaluate them on an acoustic word discrimination task. We identify several embedding algorithms that match or improve upon the DTW baseline in low-resource settings.
Familia y desarrollo psicológico

Los autores son reconocidos expertos en este apasionante tema y proceden de la Universidad del País Vasco y de la Universidad de Sevilla. Han acordado ceder sus derechos de autor a la ONG Intermón Oxfam con el objeto de que los dedique a mejorar los contextos para el desarrollo psicológico de niños desfavorecidos y a lograr que la familia humana pueda cumplir su misión de potenciar el desarrollo de personas pacíficas, libres, solidarias y felices.
The Three-Dimensional Gauss Algorithm Is Strongly Convergent Almost Everywhere

A proof that the three-dimensional Gauss algorithm is strongly convergent almost everywhere is given. This algorithm is equivalent to Brun's algorithm and to the modified lacobi-Perron algorithm considered by Podsypanin and Schweiger. The proof involves the rigorouscomputer assisted estimation of the largest Lyapunov exponent of a cocycle associated to the algorithm. To the best of my knowledge, this is the first proof of almost everywhere strong convergence of a Jacobi-Perron type algorithm in dimension greater than two.
Screen time is associated with adiposity and insulin resistance in children

Background Higher screen time is associated with type 2 diabetes (T2D) risk in adults, but the association with T2D risk markers in children is unclear. We examined associations between self-reported screen time and T2D risk markers in children. Methods Survey of 4495 children aged 9–10 years who had fasting cardiometabolic risk marker assessments, anthropometry measurements and reported daily screen time; objective physical activity was measured in a subset of 2031 children. Results Compared with an hour or less screen time daily, those reporting screen time over 3 hours had higher ponderal index (1.9%, 95% CI 0.5% to 3.4%), skinfold thickness (4.5%, 0.2% to 8.8%), fat mass index (3.3%, 0.0% to 6.7%), leptin (9.2%, 1.1% to 18.0%) and insulin resistance (10.5%, 4.9% to 16.4%); associations with glucose, HbA1c, physical activity and cardiovascular risk markers were weak or absent. Associations with insulin resistance remained after adjustment for adiposity, socioeconomic markers and physical activity. Conclusions Strong graded associations between screen time, adiposity and insulin resistance suggest that reducing screen time could facilitate early T2D prevention. While these observations are of considerable public health interest, evidence from randomised controlled trials is needed to suggest causality.
Induction of acquired resistance to antiestrogen by reversible mitochondrial DNA depletion in breast cancer cell line

Although the net benefits of tamoxifen in adjuvant breast cancer therapy have been proven, the recurrence of the cancer in an aggressive and hormone independent form has been highly problematic. We previously demonstrated the important role mitochondrial DNA (mtDNA) plays in hormone‐independence in prostate cancer. Here, the role of mtDNA in breast cancer progression was investigated. We established hydroxytamoxifen (4‐OHT) resistant HTRMCF by growing MCF‐7, human breast adenocarcinoma cells, in the presence of 4‐OHT. HTRMCF was cross‐resistant to 4‐OHT and ICI182,780 concurrent with the depletion of mtDNA. To further investigate the role of mtDNA depletion, MCF‐7 was depleted of mtDNA by treatment with ethidium bromide. MCFρ0 was resistant to both 4‐OHT and ICI182,780. Furthermore, cybrid (MCFcyb) prepared by fusion MCFρ0 with platelet to transfer mtDNA showed susceptibility to antiestrogen. Surprisingly, after withdrawal of 4‐OHT for 8 weeks, HTRMCF and their clones became susceptible to both drugs concurrent with a recovery of mtDNA. Herein, our results substantiated the first evidence that the depletion of mtDNA induced by hormone therapy triggers a shift to acquired resistance to hormone therapy in breast cancer. In addition, we showed that mtDNA depletion can be reversed, rendering the cancer cells susceptible to antiestrogen. The fact that the hormone independent phenotype can be reversed should be a step toward more effective treatments for estrogen‐responsive breast cancer. © 2007 Wiley‐Liss, Inc.
Francisella tularensis subsp. tularensis Group A.I, United States

We used whole-genome analysis and subsequent characterization of geographically diverse strains using new genetic signatures to identify distinct subgroups within Francisella tularensis subsp. tularensis group A.I: A.I.3, A.I.8, and A.I.12. These subgroups exhibit complex phylogeographic patterns within North America. The widest distribution was observed for A.I.12, which suggests an adaptive advantage.
Role of the serotonergic system in the genesis of vasovagal syncope.

AIMS The hypotensive reflex responsible for vasovagal syncope appears related to a reduction in sympathetic neural outflow. Several animal studies suggest that serotonin may play a role in the genesis of this reflex, through inhibition of sympathetic activity. However, the role of the serotonergic system is unknown in humans. The purpose of the study was to investigate the role of the serotonergic system in the genesis of vasovagal syncope by means of the level of platelet and plasma serotonin, as well as plasma catecholamines, during tilt-induced syncope. METHODS AND RESULTS Fifteen patients (age 34 +/- 16 years) with vasovagal syncope underwent a head-up tilt test (HUT, 60 degrees , 45 min). If syncope did not develop, 300 microg nitroglycerin was administered sublingually and patients continued to be tilted for a further 20 min. Blood samples were obtained in the supine position, and then after 3, 10, 15, 30, 45, 48 and 65 min of HUT. If syncope developed, blood samples were obtained at the beginning of the prodrome, during syncope and after the recovery of consciousness. Platelet and plasma serotonin and plasma catecholamines were measured using high-pressure liquid chromatography with electrochemical detection. Ten patients developed syncope during the unmedicated HUT and four after nitroglycerin. In these patients plasma adrenaline significantly increased from the last programmed sample before the prodrome to its beginning and showed a further increase during loss of consciousness, whereas plasma noradrenaline did not increase, as an expression of inhibition of sympathetic neural outflow. In the patients experiencing syncope, both platelet and plasma serotonin showed no significant change after tilt-up, at the beginning of prodrome, during syncope and after recovery of consciousness. CONCLUSION These results do not suggest that the serotonergic system plays a role in the pathophysiology of vasovagal syncope.
The Act Frequency Approach to Personality

The act frequency approach to personality is advanced in this article. Dispositions are viewed as summaries of act frequencies that, in themselves, possess no explanatory status. As sociocultural emergents, dispositions function as natural cognitive categories with acts as members. Category boundaries are fuzzy, and acts within each category differ in their prototypicality of membership. A series of studies focusing on indices of act trends and on a comparative analysis of the internal structure of dispositions illustrates this basic formulation. The act frequency approach is then placed within a taxonomic framework of the relations among act categories (horizontal dimension) and hierarchic classification (vertical dimension). Theoretical implications of the act frequency approach are examined. Dispositional consistency is distinguished from behavioral consistency and several act frequency indices (e.g., dispositional versatility, situational scope) are defined. Situational analysis and personality coherence are then viewed from the act frequency perspective. Discussion focuses on the possible origins and development of dispositional categories and implications of alternative middle-level constructs for act categorization and personality theory.
The Intricate Network of Adipokines and Stroke

Cerebrovascular disorders, particularly ischemic stroke, are one of the most common neurological disorders. High rates of overweight and obesity support an interest in the role of adipose tissue and adipose tissue releasing cytokines in inducing associated comorbidities. Adipokines can serve as a key messenger to central energy homeostasis and metabolic homeostasis. They can contribute to the crosstalk between adipose tissue and brain. However recent research has offered ambiguous data on the network of adipose tissue, adipokines, and vascular disorders. In our paper we provide a critical insight into the role of adipokines in evolution of ischemic stroke.
Aboveground Tree Growth Varies with Belowground Carbon Allocation in a Tropical Rainforest Environment

Young secondary forests and plantations in the moist tropics often have rapid rates of biomass accumulation and thus sequester large amounts of carbon. Here, we compare results from mature forest and nearby 15–20 year old tree plantations in lowland Costa Rica to evaluate differences in allocation of carbon to aboveground production and root systems. We found that the tree plantations, which had fully developed, closed canopies, allocated more carbon belowground - to their root systems - than did mature forest. This increase in belowground carbon allocation correlated significantly with aboveground tree growth but not with canopy production (i.e., leaf fall or fine litter production). In contrast, there were no correlations between canopy production and either tree growth or belowground carbon allocation. Enhanced allocation of carbon to root systems can enhance plant nutrient uptake, providing nutrients beyond those required for the production of short-lived tissues such as leaves and fine roots, and thus enabling biomass accumulation. Our analyses support this deduction at our site, showing that enhanced allocation of carbon to root systems can be an important mechanism promoting biomass accumulation during forest growth in the moist tropics. Identifying factors that control when, where and for how long this occurs would help us to improve models of forest growth and nutrient cycling, and to ascertain the role that young forests play in mitigating increased atmospheric carbon dioxide.
A comparison of methods to assess nursing home residents' unmet needs.

PURPOSE This paper compares three interview methodologies to assess nursing home (NH) residents' unmet needs with regard to activity of daily living (ADL) care. DESIGN AND METHODS The study was a survey of 70 residents across seven ADL care domains. The three types of interview methods included: (a) direct satisfaction questions about ADL care, (b) questions that compared residents' preferences about ADL care frequency or occurrence to perceptions of the ADL care delivered (discrepancy measure), and (c) open-ended questions that asked what residents wanted changed about ADL care. RESULTS Estimates of the proportion of residents with unmet needs were significantly higher with the discrepancy and open-ended measures as compared to the direct satisfaction measures across most ADL care domains (McNemar's Test; p <.05-p <.01). IMPLICATIONS The analysis of residents' responses to open-ended questions produced the most useful information for individualizing aspects of technical care and assessing the interpersonal quality of care, whereas the discrepancy questions elicited specific information useful for changing the frequency or occurrence of ADL care. Interview methodologies that directly ask residents questions about satisfaction with ADL care are the least useful for designing improvement interventions.