Miracle-dz/UniFAR-FTUs huggingface dataset:
Automatic Actin Filament Quantification of Osteoblasts and Their Morphometric Analysis on Microtextured Silicon-Titanium Arrays

Microtexturing of implant surfaces is of major relevance in the endeavor to improve biorelevant implant designs. In order to elucidate the role of biomaterial’s topography on cell physiology, obtaining quantitative correlations between cellular behavior and distinct microarchitectural properties is in great demand. Until now, the microscopically observed reorganization of the cytoskeleton on structured biomaterials has been difficult to convert into data. We used geometrically microtextured silicon-titanium arrays as a model system. Samples were prepared by deep reactive-ion etching of silicon wafers, resulting in rectangular grooves (width and height: 2 µm) and cubic pillars (pillar dimensions: 2 × 2 × 5 and 5 × 5 × 5 µm); finally sputter-coated with 100 nm titanium. We focused on the morphometric analysis of MG-63 osteoblasts, including a quantification of the actin cytoskeleton. By means of our novel software FilaQuant, especially developed for automatic actin filament recognition, we were first able to quantify the alterations of the actin network dependent on the microtexture of a material surface. The cells’ actin fibers were significantly reduced in length on the pillared surfaces versus the grooved array (4–5 fold) and completely reorganized on the micropillars, but without altering the orientation of cells. Our morpho-functional approach opens new possibilities for the data correlation of cell-material interactions.
Career success of older workers: the influence of social skills and continuous learning ability

Purpose – The purpose of this paper is to examine the influence of the competencies social skills and continuous learning ability on career success and career satisfaction among workers aged 50 or over. Design/methodology/approach – A mixed methods approach consisted of 920 surveys and 11 semi-structured interviews with working individuals aged 50 or above, registered at a job agency specialized in older employees in the Netherlands. Multiple regression and content analysis were used to analyze the findings. Findings – The survey showed a positive relationship between the competencies and career success and career satisfaction However, the interviews revealed that not all older workers perceived the need to continuously stay up-to-date as positive, pushing them into retirement. Originality/value – The influence of competencies on career success and career satisfaction of older workers has received only little attention from researchers.
Monoacylglycerol Lipase Activity Is a Critical Modulator of the Tone and Integrity of the Endocannabinoid System

Endocannabinoids are lipid molecules that serve as natural ligands for the cannabinoid receptors CB1 and CB2. They modulate a diverse set of physiological processes such as pain, cognition, appetite, and emotional states, and their levels and functions are tightly regulated by enzymatic biosynthesis and degradation. 2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid in the brain and is believed to be hydrolyzed primarily by the serine hydrolase monoacylglycerol lipase (MAGL). Although 2-AG binds and activates cannabinoid receptors in vitro, when administered in vivo, it induces only transient cannabimimetic effects as a result of its rapid catabolism. Here we show using a mouse model with a targeted disruption of the MAGL gene that MAGL is the major modulator of 2-AG hydrolysis in vivo. Mice lacking MAGL exhibit dramatically reduced 2-AG hydrolase activity and highly elevated 2-AG levels in the nervous system. A lack of MAGL activity and subsequent long-term elevation of 2-AG levels lead to desensitization of brain CB1 receptors with a significant reduction of cannabimimetic effects of CB1 agonists. Also consistent with CB1 desensitization, MAGL-deficient mice do not show alterations in neuropathic and inflammatory pain sensitivity. These findings provide the first genetic in vivo evidence that MAGL is the major regulator of 2-AG levels and signaling and reveal a pivotal role for 2-AG in modulating CB1 receptor sensitization and endocannabinoid tone.
Contact and Evaluations of Violence: An Intersectional Analysis of Swedish Court Orders

What are the impacts of age, ethnicity, and gender on ensuring children's rights to participation and protection in disputed contact cases in which there are indications of violence? Drawing from a review of all contested contact cases from three primary courts in Sweden during 2010 and 2011, findings suggest that the contact presumption is strong, and generally overrides protection. This norm applies even where there are convictions or explicit reports of child abuse or domestic violence. Priority was neither given to consideration of risk of abuse, nor was a view of children as competent subjects with a right to participation in these proceedings, despite both of these concepts being present in Swedish family law. In cases with 'non-Nordic' fathers however, the contact presumption is less likely to override protection than in cases with 'Nordic' fathers. Intersections of adult, male, and white privileges appear as an obstacle for ensuring children's rights.
Coordinated oncogenic transformation and inhibition of host immune responses by the PAX3-FKHR fusion oncoprotein

Tumors have evolved elaborate mechanisms for evading immune detection, such as production of immunoinhibitory cytokines and down-regulation of major histocompatibility complex (MHC) expression. We have studied PAX3-FKHR as an example of an oncogenic fusion protein associated with an aggressive metastatic cancer. We show that PAX3-FKHR alters expression of genes that are normally regulated by Janus kinase/signal transducer and activator of transcription (STAT) signaling pathways. This occurs as a result of a specific interaction between PAX3-FKHR and the STAT3 transcription factor, which results in a dramatic reduction in tumor MHC expression, and an alteration in local cytokine concentrations to inhibit surrounding inflammatory cells and immune detection. Collectively, these data show that an oncogenic transcription factor can promote tumor growth and tissue invasion while inhibiting local inflammatory and immune responses. This is the first time that an immunomodulatory role has been described for an oncogenic fusion protein.
LET'S GIVE THEM SOMETHING TO TALK ABOUT: HOW DIFFERENT TOPICS AFFECT CONFLICT COMMUNICATION BEHAVIOR

Title of Thesis: LET’S GIVE THEM SOMETHING TO TALK ABOUT: HOW DIFFERENT TOPICS AFFECT CONFLICT COMMUNICATION BEHAVIOR Adam Glenn Lowe, Master of Science, 2011 Thesis Directed by: Professor Norman B. Epstein Department of Family Science Past research suggests it is not what a conflict is about or how much conflict exists between two people, but rather how the parties interact regarding their conflicting preferences that determines whether the conflict has negative effects on their relationship. The current study examined the degrees to which couples’ communication behavior in specific situations in which they discuss a conflict-related topic is influenced by the conflict topic theme that they discuss and by their general communication patterns. Conflict topics were assessed with the Relationship Issues Survey, general communication patterns with the Communication Patterns Questionnaire, and specific communication behavior during discussions with the Marital Interaction Coding System – Global. Findings indicated several significant effects of both content area and general communication style on communication behavior. Post-hoc analyses indicated that couples discussing conflicts regarding basic life values, priorities, and consideration for one’s partner exhibited more negative communication behavior than those discussing issues regarding closeness, relationship commitment, emotional connectivity, and expressiveness. Possible implications of the findings are discussed.
Postglacial recolonization shaped the genetic diversity of the winter moth (Operophtera brumata) in Europe

Abstract Changes in climate conditions, particularly during the Quaternary climatic oscillations, have long been recognized to be important for shaping patterns of species diversity. For species residing in the western Palearctic, two commonly observed genetic patterns resulting from these cycles are as follows: (1) that the numbers and distributions of genetic lineages correspond with the use of geographically distinct glacial refugia and (2) that southern populations are generally more diverse than northern populations (the “southern richness, northern purity” paradigm). To determine whether these patterns hold true for the widespread pest species the winter moth (Operophtera brumata), we genotyped 699 individual winter moths collected from 15 Eurasian countries with 24 polymorphic microsatellite loci. We find strong evidence for the presence of two major genetic clusters that diverged ~18 to ~22 ka, with evidence that secondary contact (i.e., hybridization) resumed ~ 5 ka along a well‐established hybrid zone in Central Europe. This pattern supports the hypothesis that contemporary populations descend from populations that resided in distinct glacial refugia. However, unlike many previous studies of postglacial recolonization, we found no evidence for the “southern richness, northern purity” paradigm. We also find evidence for ongoing gene flow between populations in adjacent Eurasian countries, suggesting that long‐distance dispersal plays an important part in shaping winter moth genetic diversity. In addition, we find that this gene flow is predominantly in a west‐to‐east direction, suggesting that recently debated reports of cyclical outbreaks of winter moth spreading from east to west across Europe are not the result of dispersal.
The Nature and Correlates of Psychological/Emotional Functioning among a Sample of Detained Youths

This study explores the relationships between a sample of detained juveniles' specific problems and their psychological orientations. It was proposed that for youths who are in trouble with the law, specific behavioral difficulties (e.g., substance abuse) would be associated with the youths' psychological orientation to a deviant lifestyle. Analysis of a sample of 145 status offender and juvenile delinquent cases revealed a significant positive association among “antisocial” orientation and physical abuse, illicit drug use, repeated placements in secure detention, and self-derogation. These relationships held when controlling for the effects of gender, age, and ethnicity. These results support the view that specific behavior problems are related to a valuation of a deviant lifestyle. The implications of these results for service providers are discussed.
Positive selection at the protein network periphery: Evaluation in terms of structural constraints and cellular context

Because of recent advances in genotyping and sequencing, human genetic variation and adaptive evolution in the primate lineage have become major research foci. Here, we examine the relationship between genetic signatures of adaptive evolution and network topology. We find a striking tendency of proteins that have been under positive selection (as compared with the chimpanzee) to be located at the periphery of the interaction network. Our results are based on the analysis of two types of genome evolution, both in terms of intra- and interspecies variation. First, we looked at single-nucleotide polymorphisms and their fixed variants, single-nucleotide differences in the human genome relative to the chimpanzee. Second, we examine fixed structural variants, specifically large segmental duplications and their polymorphic precursors known as copy number variants. We propose two complementary mechanisms that lead to the observed trends. First, we can rationalize them in terms of constraints imposed by protein structure: We find that positively selected sites are preferentially located on the exposed surface of proteins. Because central network proteins (hubs) are likely to have a larger fraction of their surface involved in interactions, they tend to be constrained and under negative selection. Conversely, we show that the interaction network roughly maps to cellular organization, with the periphery of the network corresponding to the cellular periphery (i.e., extracellular space or cell membrane). This suggests that the observed positive selection at the network periphery may be due to an increase of adaptive events on the cellular periphery responding to changing environments.
Innate Immunity, Decidual Cells, and Preeclampsia

Preeclampsia (PE) manifested by hypertension and proteinuria complicates 3% to 8% of pregnancies and is a leading cause of fetal–maternal morbidity and mortality worldwide. It may lead to intrauterine growth restriction, preterm delivery, and long-term sequelae in women and fetuses, and consequently cause socioeconomic burden to the affected families and society as a whole. Balanced immune responses are required for the maintenance of successful pregnancy. Although not a focus of most studies, decidual cells, the major resident cell type at the fetal–maternal interface, have been shown to modulate the local immune balance by interacting with other cell types, such as bone marrow derived-immune cells, endothelial cells, and invading extravillous trophoblasts. Accumulating evidence suggests that an imbalanced innate immunity, facilitated by decidual cells, plays an important role in the pathogenesis of PE. Thus, this review will discuss the role of innate immunity and the potential contribution of decidual cells in the pathogenesis of PE.
Independence among physiological traits suggests flexibility in the face of ecological demands on phenotypes

Phenotypic flexibility allows animals to adjust their physiology to diverse environmental conditions encountered over the year. Examining how these varying traits covary gives insights into potential constraints or freedoms that may shape evolutionary trajectories. In this study, we examined relationships among haematocrit, baseline corticosterone concentration, constitutive immune function and basal metabolic rate in red knot Calidris canutus islandica individuals subjected to experimentally manipulated temperature treatments over an entire annual cycle. If covariation among traits is constrained, we predict consistent covariation within and among individuals. We further predict consistent correlations between physiological and metabolic traits if constraints underlie species‐level patterns found along the slow‐fast pace‐of‐life continuum. We found no consistent correlations among haematocrit, baseline corticosterone concentration, immune function and basal metabolic rate either within or among individuals. This provides no evidence for constraints limiting relationships among these measures of the cardiovascular, endocrine, immune and metabolic systems in individual red knots. Rather, our data suggest that knots are free to adjust individual parts of their physiology independently. This makes good sense if one places the animal within its ecological context where different aspects of the environment might put different pressures on different aspects of physiology.
Organizational resistance as a vector of deterritorialization: The case of WikiLeaks and secrecy havens

This article investigates the relations of power and resistance manifest by the WikiLeaks network. The primary research question of this inquiry is, ‘what power relations and possibilities for resistance are presented by WikiLeaks as a novel form of network organization? ’ The article shows that WikiLeaks has been able to exert influence from the periphery of existing networks by exploiting vectors of ‘deterritorialization’ to destabilize existing power relations. The article contributes to the literature on the network organization by developing an account of resistance to State and corporate power in terms of an ‘absolute deterritorialization’. This idea has important implications for the tactics of resistance in network organizations, where vectors of deterritorialization have become a defining feature of resistance tactics of the WikiLeaks network.
Restoration of the Orienting Response to a Light by a Change in its Predictive Accuracy

In three experiments the orienting response (OR) to a light was reduced by presenting rats with a serial conditioning schedule in which the light was always followed by a tone, and the tone was intermittently paired with food. The original strength of the OR was then restored by changing the nature of the serial conditioning: the light was followed either by the tone and food, or by nothing. Experiments 1 and 2 demonstrated that this change resulted in an increase in the OR relative to control groups, which continued to receive the original training. Experiment 3 revealed a similar effect using a within-subjects design. A further finding from the first two experiments was that the recovery of the OR was accompanied by an enhancement in the conditionability of the light. Overall, the results suggest that the accuracy with which the light signals the events that immediately follow it is an important determinant of the strength of the OR it elicits.
The environments of FRII radio sources

Using ROSAT observations, we estimate gas pressures in the X-ray-emitting medium surrounding 63 FRII radio galaxies and quasars. We compare these pressures with the internal pressures of the radio-emitting plasma estimated by assuming minimum energy or equipartition. In the majority of cases (including 12/13 sources with modelled, spatially resolved X-ray emission) radio sources appear to be underpressuredwith respect to the external medium, suggesting that simple minimum-energy arguments underestimate the sources’ internal energy density. We discuss possible departures from the minimum energy condition and the consequences of our result for models of the dynamics of radio galaxies, in particular self-similar models (Kaiser & Alexander 1997).
Incremental Parsing Models for Dialog Task Structure

In this paper, we present an integrated model of the two central tasks of dialog management: interpreting user actions and generating system actions. We model the interpretation task as a classification problem and the generation task as a prediction problem. These two tasks are interleaved in an incremental parsing-based dialog model. We compare three alternative parsing methods for this dialog model using a corpus of human-human spoken dialog from a catalog ordering domain that has been annotated for dialog acts and task/subtask information. We contrast the amount of context provided by each method and its impact on performance.
Subthreshold Corazol Doses Induced Generalized Seizures in Audigenic Seizure-Prone Rats

Purpose: It was suggested that high audiogenic epilepsy (AE) susceptibility in rats of Krushinsky-Molodkina (KM) rat strain could be accompanied by increase in general seizure proneness. This could be tested exploring the reaction to low corazol doses in these rats and in rats of genetically related but non-prone to AE strain (strain “0”), which was used as control,. Method: 6 months old male ratsof two strains (KM and received the subthreshold (40 mg/kg) corazol injections. After injections rats were placed in individual cages and were observed for 40 - 50 min in order to discover the display/absence of corazol induced convulsions. Results: The typical corazol seizures developed in more than 50% of KM rats (AE-susceptible) in comparison to non-prone control rats, in which these seizures were more rare events. The control rats of “0” strain demonstrated seizures of significantly lower inten sity, than in KM rats. Although most rats of “0”, which did not express corazol seizures and no AE seizures three weeks before the experiment as well, they demonstrated the intense AE seizures when tested 20 min after corazol injection. Conclusions: Rats of KM strain, for intense AE seizures, developed generalized seizures in response to subthreshold corazol dose, demonstrating the elevated general seizure proneness
Lateral Asymmetry in the Hippocampal Response to the Remoteness of Autobiographical Memories

The time scale of hippocampal involvement in retrieving memories, particularly those more remote, is still a matter of debate. Some propose that the hippocampus is not involved in the retrieval of remote memories, whereas others assert that it is necessary for memory retrieval in perpetuity. Functional magnetic resonance imaging was used to examine the effect of remoteness on the neural basis of memory. We used a parametric event-related random-effects design in a large group of subjects to overcome some of the limitations of previous neuroimaging studies. We found that the hippocampi were significantly active during the retrieval of autobiographical memories. Notably, the two hippocampi diverged in their responses to remoteness. The right hippocampus showed a temporal gradient, decreasing in activity the more remote the autobiographical memories. No such effect was apparent in the left hippocampus, suggesting its invariant involvement in remembering autobiographical events throughout the lifespan. The dorsal amygdalas showed a temporal gradient similar to the right hippocampus, but emotional valence and intensity were not directly associated with changes in activity. The current results indicate that consideration of lateral asymmetry may help to broaden the scope of theoretical interpretations concerning hippocampal involvement in remote memory.
A systematic review of combination step III opioid therapy in cancer pain: An EPCRC opioid guideline project

Background: The use of combinations of opioids is a common clinical practice; however, this is not advocated by the World Health Organization (WHO) analgesic ladder. As opioid combination therapy becomes used increasingly, a review of the evidence on this practice was conducted. Aims: To carry out a systematic review of the use of strong opioids in combination in cancer pain. Methods: The following databases were searched electronically: Embase (1980–2010 week 2), Medline (1950–2010 week 1) and the Cochrane Database of Systematic Reviews (fourth quarter 2009). Only strong opioids as defined by the WHO ladder and full opioid agonists were examined. Only studies conducted in human, adult patients with chronic cancer pain were eligible. Studies must have contained data on efficacy and/or side effects in the key point. Appraisal was conducted using predetermined criteria set by the EAPC guideline development group. All potential papers were reviewed independently by both authors. Results: In total 596 articles were retrieved resulting in only two eligible studies, which were rated as grade C and grade D evidence. These examined morphine in combination with oxycodone or fentanyl/methadone. Conclusion: Only a weak recommendation can be used to support combination opioid therapy. This recommendation is also based on the caveat that the desirable effects of combination opioid therapy is outweighed by any disadvantages that this would confer. Prospective randomized trials are needed to clarify the benefits and safety of combination opioid therapy.