Miracle-dz/UniFAR-FTUs huggingface dataset:
Mechanistic characterization of a copper containing thiosemicarbazone with potent antitumor activity

Background The thiosemicarbazone CD 02750 (VLX50) was recently reported as a hit compound in a phenotype-based drug screen in primary cultures of patient tumor cells. We synthesized a copper complex of VLX50, denoted VLX60, and characterized its antitumor and mechanistic properties. Materials and Methods The cytotoxic effects and mechanistic properties of VLX60 were investigated in monolayer cultures of multiple human cell lines, in tumor cells from patients, in a 3-D spheroid cell culture system and in vivo and were compared with those of VLX50. Results VLX60 showed ≥ 3-fold higher cytotoxic activity than VLX50 in 2-D cultures and, in contrast to VLX50, retained its activity in the presence of additional iron. VLX60 was effective against non-proliferative spheroids and against tumor xenografts in vivo in a murine model. In contrast to VLX50, gene expression analysis demonstrated that genes associated with oxidative stress were considerably enriched in cells exposed to VLX60 as was induction of reactive oxygen. VLX60 compromised the ubiquitin-proteasome system and was more active in BRAF mutated versus BRAF wild-type colon cancer cells. Conclusions The cytotoxic effects of the copper thiosemicarbazone VLX60 differ from those of VLX50 and shows interesting features as a potential antitumor drug, notably against BRAF mutated colorectal cancer.
Antiangiogenic Potential of Microbial Metabolite Elaiophylin for Targeting Tumor Angiogenesis

Angiogenesis plays a very important role in tumor progression through the creation of new blood vessels. Therefore, angiogenesis inhibitors could contribute to cancer treatment. Here, we show that a microbial metabolite, elaiophylin, exhibits potent antiangiogenic activity from in vitro and in vivo angiogenesis assays. Elaiophylin dramatically suppressed in vitro angiogenic characteristics such as proliferation, migration, adhesion, invasion and tube formation of human umbilical vein endothelial cells (HUVECs) stimulated by vascular endothelial growth factor (VEGF) at non-toxic concentrations. In addition, elaiophylin immensely inhibited in vivo angiogenesis of the chorioallantoic membrane (CAM) from growing chick embryos without cytotoxicity. The activation of VEGF receptor 2 (VEGFR2) in HUVECs by VEGF was inhibited by elaiophylin, resulting in the suppression of VEGF-induced activation of downstream signaling molecules, Akt, extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38, nuclear factor-κB (NFκB), matrix metalloproteinase (MMP)-2 and -9 which are closely associated with VEGF-induced angiogenesis. We also found that elaiophylin blocked tumor cell-induced angiogenesis both in vitro and in vivo. Elaiophylin downregulated the expression of VEGF by inhibiting hypoxia inducible factor-1α (HIF-1α) accumulation in tumor cells. To our knowledge, these results for the first time demonstrate that elaiophylin effectively inhibits angiogenesis and thus may be utilized as a new class of natural antiangiogenic agent for cancer therapy.
In silico analysis of the competition between Streptococcus sanguinis and Streptococcus mutans in the dental biofilm

During dental caries, the dental biofilm modifies the composition of the hundreds of involved bacterial species. Changing environmental conditions influence competition. A pertinent model to exemplify the complex interplay of the microorganisms in the human dental biofilm is the competition between Streptococcus sanguinis and Streptococcus mutans. It has been reported that children and adults harbor greater numbers of S. sanguinis in the oral cavity, associated with caries-free teeth. Conversely, S. mutans is predominant in individuals with a high number of carious lesions. Competition between both microorganisms stems from the production of H2 O2 by S. sanguinis and mutacins, a type of bacteriocins, by S. mutans. There is limited evidence on how S. sanguinis survives its own H2 O2 levels, or if it has other mechanisms that might aid in the competition against S. mutans, nonetheless. We performed a genomic and metabolic pathway comparison, coupled with a comprehensive literature review, to better understand the competition between these two species. Results indicated that S. sanguinis can outcompete S. mutans by the production of an enzyme capable of metabolizing H2 O2 . S. mutans, however, lacks the enzyme and is susceptible to the peroxide from S. sanguinis. In addition, S. sanguinis can generate energy through gluconeogenesis and seems to have evolved different communication mechanisms, indicating that novel proteins may be responsible for intra-species communication.
Short-term efficacy of a web-based computer-tailored nutrition intervention: Main Effects and Mediators

Background: This study evaluates the short-term efficacy and respondents’ evaluations of a Web-based computer-tailored nutrition intervention, aiming to decrease saturated fat intake and increase fruit and vegetable intake. Perceived personal relevance, individualization, and interestingness of the information were tested as mediators of the effects of the tailored intervention.Purpose: The objective was to study the short-term effects of a Web-based computer-tailored nutrition intervention.Methods: Respondents (N = 782) were randomly assigned to a tailored intervention group, a generic nutrition information control group, or a no-information control group. Fat, fruit, and vegetable intakes and behavioral determinants were measured at baseline and at 3 weeks postintervention. Posttest group differences were determined by multiple linear regression analyses.Results: The computer-tailored intervention produced significant effects for the determinants of fat, fruit, and vegetable intake and for vegetable and fruit intake. The tailored information was rated as more personally relevant, individualized, interesting, and new than the generic nutrition information. Perceived personal relevance, individualization, and interestingness were identified as mediators of some of the tailoring effects.Conclusions: The findings of this study indicate that Web-based, computer-tailored nutrition information can have a short-term effect on the determinants of fat, fruit, and vegetable intake. The effect of the tailored information may be partly explained by the perceived personal relevance and individualization of the information.
Effects of CO 2 on particle size distribution and phytoplankton abundance during a mesocosm bloom experiment (PeECE II)

The influence of seawater carbon dioxide (CO2) concentration on the size distribution of suspended particles (2–60 μm) and on phytoplankton abundance was investigated during a mesocosm experiment at the large scale facility (LFS) in Bergen, Norway, in the frame of the Pelagic Ecosystem CO2 Enrichment study (PeECE II). In nine outdoor enclosures the partial pressure of CO2 in seawater was modified by an aeration system to simulate past (~190 μatm CO2), present (~370 μatm CO2) and future (~700 μatm CO2) CO2 conditions in triplicates. Due to the initial addition of inorganic nutrients, phytoplankton blooms developed in all mesocosms and were monitored over a period of 19 days. Seawater samples were collected daily for analysing the abundance of suspended particles and phytoplankton with the Coulter Counter and with Flow Cytometry, respectively. During the bloom period, the abundance of small particles (<4 μm) significantly increased at past, and decreased at future CO2 levels. At that time, a direct relationship between the total-surface-to-total-volume ratio of suspended particles and DIC concentration was determined for all mesocosms. Significant changes with respect to the CO2 treatment were also observed in the phytoplankton community structure. While some populations such as diatoms seemed to be insensitive to the CO2 treatment, others like Micromonas spp. increased with CO2, or showed maximum abundance at present day CO2 (i.e. Emiliania huxleyi). The strongest response to CO2 was observed in the abundance of small autotrophic nano-plankton that strongly increased during the bloom in the past CO2 mesocosms. Together, changes in particle size distribution and phytoplankton community indicate a complex interplay between the ability of the cells to physiologically respond to changes in CO2 and size selection. Size of cells is of general importance for a variety of processes in marine systems such as diffusion-limited uptake of substrates, resource allocation, predator-prey interaction, and gravitational settling. The observed changes in particle size distribution are therefore discussed with respect to biogeochemical cycling and ecosystem functioning.
Infection Control Practices in U.S. Burn Units

The purpose of this study was to determine infection control procedures most commonly used in burn units in the United States. We used two data collection formats: a brief quantitative survey and a brief qualitative interview. Our response rate was 75% (104/139). We found wide variability in general infection control practices as well as in wound care practices in intensive care units. The majority of units reported having written infection control guidelines specific to the burn unit. Respondents noted challenges that burn injury presents to infection control. A broad range of techniques for preventing device- and procedure-related infection also were provided by respondents. Compliance with hand washing on the part of physicians and ancillary staff remains a major challenge. To improve the quality of infection control and reduce variation in practice, we must continue to monitor the types of practices that are delivered, provide feedback to providers on how they care for their patients, where evidence exists disseminate best practices, and build the evidence base through efficacy trials.
Asymptotically optimal inspection planning using systems with differential constraints

This paper proposes a new inspection planning algorithm, called Random Inspection Tree Algorithm (RITA). Given a perfect model of a structure, sensor specifications, robot's dynamics, and an initial configuration of a robot, RITA computes the optimal inspection trajectory that observes all points on the structure. Many inspection planning algorithms have been proposed, most of them consist of two sequential steps. In the first step, they compute a small set of observation points such that each point on the structure is visible. In the second step, they compute the shortest trajectory to visit all observation points at least once. The robot's kinematic and dynamic constraints are taken into account only in the second step. Thus, when the robot has differential constraints and operates in cluttered environments, the observation points may be difficult or even infeasible to reach. To alleviate this difficulty, RITA computes both observation points and the trajectory to visit the observation points simultaneously. RITA uses sampling-based techniques to find admissible trajectories with decreasing cost. Simulation results for 2-D environments are promising. Furthermore, we present analysis on the probabilistic completeness and asymptotic optimality of our algorithm.
Design and simulations of low cost and low magnetic field MRI system

The paper proposes the design of a low cost and low magnetic field MRI system. The idea behind the low cost and low magnetic field is that the system can be taken to and operated at remote locations suitably. The proposed design operates at a maximum field intensity of 0.2 T. The main magnetic field is proposed to be generated Helmholtz coils based on copper wires as a winding. The strength of gradient magnetic field has been at 75μT. The gradient has been generated using Maxwell coils and Saddle coils. Simulation study has been done where the radius of the Helmholtz coil has been kept at 30cm and the gradient coil has been kept at a radius of 25cm. The RF coil is designed with a radius of 20cm a the resonant frequency of 8.513MHz for 0.2T main magnetic field. Simulation studies performed with COMSOL Multiphysics 5.2 reveals homogeneity of main magnetic field within the coil. The homogeneity of magnetic field at the center of Helmholtz coil for one slice of volume has found to be ±1.2 percent.
A Very Low Loss 220–325 GHz Silicon Micromachined Waveguide Technology

This letter reports for the first time on a very low loss silicon micromachined waveguide technology, implemented for the frequency band of 220–325 GHz. The waveguide is realized by utilizing a double H-plane split in a three-wafer stack. This ensures very low surface roughness, in particular on the top and bottom surfaces of the waveguide, without the use of any surface roughness reduction processing steps. This is superior to previous micromachined waveguide concepts, including E-plane and single H-plane split waveguides. The measured average surface roughness is 2.14 nm for the top/bottom of the waveguide, and 163.13 nm for the waveguide sidewalls. The measured insertion loss per unit length is 0.02–0.07 dB/mm for 220–325 GHz, with a gold layer thickness of 1 <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>m on the top/bottom and 0.3 <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>m on the sidewalls. This represents, in this frequency band, the lowest loss for any silicon micromachined waveguide published to date and is of the same order as the best metal waveguides.
Depth attenuation of organic matter export associated with jelly falls

We explore the attenuation in the export ratio of jelly‐POM (particulate organic matter) with depth as a function of the decay rate, temperature, and sedimentation rate. Using data from the Vertical Transport In the Global Ocean project, we compare ratios computed with the Martin‐curve, with a particle‐based parameterization, and with sediment‐trap data. Owing to the temperature dependence of the decay rate (Q10 = 4.28), the jelly‐POM export ratio below 500 m is 20–45% larger in subpolar and temperate areas than in the tropics. Vertical migration of gelatinous zooplankton leads to a variable starting depth of a jelly fall (death depth), which governs the start of remineralization, and the fate of the biomass. Owing to the absence of observations, we employ a sinking speed matrix ranging from 100 m d‐1 to 1500 m d‐1 to represent slow‐ and fast‐sinking carcasses. The assumption of a constant decay rate k independent of temperature in other particle‐based models may not be appropriate. These results provide information for including jelly‐POM in global biogeochemical model formulations.
The Differential Diagnosis and Treatment of Catatonia in Children and Adolescents

Learning objectivesAfter participating in this activity, learners should be better able to:• Assess the etiologies associated with catatonia in children and adolescents• Evaluate the differential diagnosis of pediatric catatonia• Interpret the literature regarding the treatment of children and adolescents with catatonia ObjectivePediatric catatonia is associated with many medical and psychiatric conditions. Mortality is high, and proper treatment can be lifesaving. Catatonia is increasingly recognized in pediatric populations, in which about 20% of cases are related to underlying medical conditions. To minimize morbidity, clinicians must rule out underlying disorders while simultaneously managing symptoms and causes. In our review we discuss (1) recommendations to aid rapid decision making, both diagnostic and therapeutic, (2) emergent conditions and management, (3) disorders associated with pediatric catatonia, including developmental, acquired, idiopathic, and iatrogenic etiologies, (4) available treatments, and (5) medicolegal considerations. MethodsInitial PubMed search without date constraints using MeSH terms related to pediatric catatonia, with subsequent searches on pertinent subtopics using PubMed and Google Scholar. ResultsPediatric catatonia is a dangerous but treatable neuropsychiatric condition. Psychiatrists need to be aware of differential diagnoses and to be able determine appropriate treatment within a short time frame. With prompt diagnosis and treatment, outcomes can be optimized. ConclusionPediatric catatonia is underdiagnosed and requires rapid evaluation and management.
Efficacy of an over-the-scope clip for preventing adverse events after duodenal endoscopic submucosal dissection: a prospective interventional study

Abstract Background Duodenal endoscopic submucosal dissection (ESD) remains technically challenging, with a high risk of severe adverse events. Because exposure of the duodenal post-ESD mucosal defect to pancreatic juice and bile acid reportedly induces delayed perforation and bleeding, we examined whether defect closure using an over-the-scope clip (OTSC) system was useful for preventing postoperative adverse events. Methods From April 2016 to February 2017, a total of 50 consecutive patients with superficial non-ampullary duodenal epithelial tumors (SNADETs) larger than 10 mm, with no more than semi-circumferential spread, were prospectively enrolled in this study. All of the lesions were treated by experienced ESD operators and the post-ESD mucosal defect was closed using OTSCs. Results All of the SNADETs were completely removed by ESD, with an R0 resection rate of 88.0 %. The mean procedure and closure times were 67.3 ± 58.8 minutes and 9.8 ± 7.2 minutes, respectively. Although complete defect closure was achieved in 94.0 % of the patients (47/50), two patients required surgical conversion. Delayed perforation occurred in only one patient (2.1 %), who did not have successful closure of the defect, as misplacement of the OTSC exposed the muscle layer. Meanwhile, delayed bleeding occurred in three patients (6.3 %); however, the bleeding was easily controlled using endoscopic coagulation. The mean duration of postoperative hospitalization was 5.5 ± 7.2 days. Conclusions Prophylactic defect closure using OTSCs may be effective in reducing severe adverse events after duodenal ESD.
The influence of Dekkera bruxellensis on the transcriptome of Saccharomyces cerevisiae and on the aromatic profile of synthetic wine must

A double compartment membrane system was constructed in order to systematically study possible microbial interactions between yeasts Saccharomyces cerevisiae and Dekkera bruxellensis and their impact on wine aroma. The presence of D. bruxellensis induced 77 transcripts of S. cerevisiae. These were mostly of unknown function; however, some were involved in thiamine biosynthesis and in amino acid and polyamine transport, suggesting a competitive relationship between the two yeast species. Among the transcripts with no biological function, 14 of them were found to be the members of the PAU gene family that is associated with response to anaerobiosis stress. In separated cultures, S. cerevisiae produced glycerol which was subsequently consumed by D. bruxellensis. The concentration of ethylphenols was reduced and we assume that they were absorbed onto the surfaces of S. cerevisiae yeast walls. Also in separated cultures, D. bruxellensis formed a typical profile of aromatic esters with decreased levels of acetate esters and increased level of ethyl esters.
From both sides: Participant and facilitator perceptions of SMART Recovery groups

INTRODUCTION AND AIMS The Self-Management and Recovery Training (SMART Recovery) program provides facilitated mutual aid for people with addictions. To date, little research has examined SMART Recovery. This paper examined participant and facilitator perceptions of the helpfulness of cognitive behaviour therapy tools in SMART Recovery groups. SMART Recovery's strengths and areas for improvement were also explored, as well as overall participant satisfaction with SMART Recovery. DESIGN AND METHODS This exploratory study was conducted as part of the first national survey of SMART Recovery in Australia. Paper surveys were posted to all registered SMART Recovery groups for participants. SMART Recovery facilitators were emailed a link to an online survey. RESULTS Overall, satisfaction with SMART Recovery was moderate to strong. Participants and facilitators perceived the cognitive behaviour therapy tools incorporated within SMART Recovery to be helpful. Participants and facilitators nominated the group experience and the SMART Recovery tools and strategies as helpful aspects of SMART Recovery. Participants and facilitators were concerned with improving public knowledge about SMART Recovery groups, updating the structure and content of SMART Recovery groups, and increasing training for facilitators. DISCUSSION AND CONCLUSIONS SMART Recovery displays strengths as communicated by those who utilise its services. However, there are opportunities to continue to improve SMART Recovery. Updating the training for facilitators and increasing communication between SMART Recovery Australia's head office and its facilitators may serve to improve service delivery. Future research should focus on examining the efficacy of SMART Recovery groups on participant outcomes. [Kelly PJ, Raftery D, Deane FP, Baker AL, Hunt D, Shakeshaft A. From both sides: Participant and facilitator perceptions of SMART Recovery groups. Drug Alcohol Rev 2017;36:325-332].
Increased Price Markup from Union Coordination – OECD Panel Evidence

Abstract Although coordination of wage bargaining probably affects entry barriers and competition in product markets, research on price determination has typically not considered such factors. We argue that the price markup depends on wage setting institutions and present empirical evidence in form of estimated price equations in a panel of 15 OECD countries. The estimates show that consumer prices may be as much as 21 percent higher in coordinated compared to uncoordinated countries, solely due to the effect of coordination on the markup. Since other studies find that coordination has a dampening effect on wages, this may explain why there is no clear effect of coordination on unemployment.
The Development of Wrap-Up Processes in Text Reading: A Study of Children’s Eye Movements

Reading comprehension is the product of constructing a coherent mental model of a text. Although some of the processes that are necessary to construct such a mental model are executed incrementally, others are deferred to the end of the clause or sentence, where integration processing is wrapped up before the reader progresses further in the text. In this longitudinal study of 65 German-speaking children across Grades 2, 3, and 4, we investigated the development of wrap-up processes at clause and sentence boundaries by tracking the children’s eye movements while they read age-appropriate texts. Our central finding was that children in Grade 2 showed strong wrap-up effects that then slowly decreased across school grades. Children in Grades 3 and 4 also increasingly used clause and sentence boundaries to initiate regressions and rereading. Finally, children in Grade 2 were shown to be significantly disrupted in their reading at line breaks, which are inherent in continuous text. This disruption decreased as the children progressed to Grades 3 and 4. Overall, our results show that children exhibit an adultlike pattern of wrap-up effects by the time they reach Grade 4. We discuss this developmental trajectory in relation to models of text processing and mechanisms of eye-movement control.