Miracle-dz/UniFAR-FTUs huggingface dataset:
Circulating V(alpha24+) Vbeta11+ NKT cell numbers are decreased in a wide variety of diseases that are characterized by autoreactive tissue damage.

Natural killer T (NKT) cells have been implicated as playing an important role in regulating immune responses. Defects in the NKT cell population were reported in animal autoimmune disease models and in distinct human autoimmune diseases. Here, we report that circulating V(alpha24+) Vbeta11+ NKT cell numbers are decreased in a broad variety of disorders with (auto)immune-mediated pathology, affecting the skin, bowel, central nervous system, and joints, regardless of disease duration or activity. Remarkably, normal circulating V(alpha24+) Vbeta11+ NKT cell numbers were found in Graves disease and coeliac disease. Since earlier studies noted a rise in NKT cells in myasthenia gravis, the picture emerges in which a defective NKT cell population is associated with autoreactive tissue damage rather than with the propensity to develop autoimmune disease. The present data support the idea that therapies aiming at the in vivo expansion of regulatory NKT cells might help to control immune-mediated damage in autoimmune disease.
Beamformer Sensitivity to Microphone Manufacturing Tolerances

One of the major problems in achieving and assuring the estimated parameters of microphone arrays during mass production is the mismatch between capturing channels, caused primarily by the variations in the manufacturing tolerances of the microphones that are used. This paper presents a methodology and the results of sensitivity analysis of beamformer parameters to these variations. A Monte Carlo method is used to simulate the mismatch in microphone responses. The beamformer noise gain and directivity index are used for evaluation. The results of the analysis justify taking measures to ensure channel matching by selecting microphones with close parameters, or by calibrating the microphones. This paper presents the requirements for microphone selection and calibration.
Criteria for measures of quantum correlations

Entanglement does not describe all quantum correlations and several authors have shown the need to go beyond entanglement when dealing with mixed states. Various different measures have sprung up in the literature, for a variety of reasons, to describe bipartite and multipartite quantum correlations; some are known under the collective name quantum discord. Yet, in the same sprit as the criteria for entanglement measures, there is no general mechanism that determines whether a measure of quantum and classical correlations is a proper measure of correlations. This is partially due to the fact that the answer is a bit muddy. In this article we attempt tackle this muddy topic by writing down several criteria for a "good" measure of correlations. We breakup our list into necessary, reasonable, and debatable conditions. We then proceed to prove several of these conditions for generalized measures of quantum correlations. However, not all conditions are met by all measures; we show this via several examples. The reasonable conditions are related to continuity of correlations, which has not been previously discussed. Continuity is an important quality if one wants to probe quantum correlations in the laboratory. We show that most types of quantum discord are continuous but none are continuous with respect to the measurement basis used for optimization.
GADD45A Does Not Promote DNA Demethylation

Although DNA methylation patterns in somatic cells are thought to be relatively stable, they undergo dramatic changes during embryonic development, gametogenesis, and during malignant transformation. The enzymology of DNA methyltransferases is well understood, but the mechanism that removes methylated cytosines from DNA (active DNA demethylation) has remained enigmatic. Recently, a role of the growth arrest and DNA damage inducible protein GADD45A in DNA demethylation has been reported [1]. We have investigated the function of GADD45A in DNA demethylation in more detail using gene reactivation and DNA methylation assays. Contrary to the previous report, we were unable to substantiate a functional role of GADD45A in DNA demethylation. The mechanism of active DNA demethylation in mammalian cells remains unknown.
Write off-loading: Practical power management for enterprise storage

In enterprise data centers power usage is a problem impacting server density and the total cost of ownership. Storage uses a significant fraction of the power budget and there are no widely deployed power-saving solutions for enterprise storage systems. The traditional view is that enterprise workloads make spinning disks down ineffective because idle periods are too short. We analyzed block-level traces from 36 volumes in an enterprise data center for one week and concluded that significant idle periods exist, and that they can be further increased by modifying the read/write patterns using write off-loading. Write off-loading allows write requests on spun-down disks to be temporarily redirected to persistent storage elsewhere in the data center. The key challenge is doing this transparently and efficiently at the block level, without sacrificing consistency or failure resilience. We describe our write off-loading design and implementation that achieves these goals. We evaluate it by replaying portions of our traces on a rack-based testbed. Results show that just spinning disks down when idle saves 28--36% of energy, and write off-loading further increases the savings to 45--60%.
Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane

Defined mutations in the mitochondrial ADP/ATP carrier (AAC) are associated with certain types of progressive external ophthalmoplegia. AAC is required for oxidative phosphorylation (OXPHOS), and dysregulation of AAC has been implicated in apoptosis. Little is known about the AAC interactome, aside from a known requirement for the phospholipid cardiolipin (CL) and that it is thought to function as a homodimer. Using a newly developed dual affinity tag, we demonstrate that yeast AAC2 physically participates in several protein complexes of distinct size and composition. The respiratory supercomplex and several smaller AAC2-containing complexes, including other members of the mitochondrial carrier family, are identified here. In the absence of CL, most of the defined interactions are destabilized or undetectable. The absence of CL and/or AAC2 results in distinct yet additive alterations in respiratory supercomplex structure and respiratory function. Thus, a single lipid can significantly alter the functional interactome of an individual protein.
Synthesis of Tetrahexahedral Platinum Nanocrystals with High-Index Facets and High Electro-Oxidation Activity

The shapes of noble metal nanocrystals (NCs) are usually defined by polyhedra that are enclosed by {111} and {100} facets, such as cubes, tetrahedra, and octahedra. Platinum NCs of unusual tetrahexahedral (THH) shape were prepared at high yield by an electrochemical treatment of Pt nanospheres supported on glassy carbon by a square-wave potential. The single-crystal THH NC is enclosed by 24 high-index facets such as {730}, {210}, and/or {520} surfaces that have a large density of atomic steps and dangling bonds. These high-energy surfaces are stable thermally (to 800°C) and chemically and exhibit much enhanced (up to 400%) catalytic activity for equivalent Pt surface areas for electro-oxidation of small organic fuels such as formic acid and ethanol.
Thrombophilia and first arterial ischaemic stroke: a systematic review

Aims: To undertake a systematic review of the literature reporting the prevalence of thrombophilia in children with a first arterial ischaemic stroke (AIS). Methods: Systematic review of case-control studies reporting data for prevalence of protein C, S, and antithrombin (AT) deficiencies, activated protein C resistance (APCr), total plasma homocysteine >95th centile, the thrombophilic mutations factor V1691 GA, prothrombin 20210GA, and MTHFR C677T in children with first, radiologically confirmed, AIS. Results: Of 1437 potentially relevant citations, 18 met inclusion criteria. A total of 3235 patients and 9019 controls had been studied. Results of meta-analyses were expressed as pooled odds ratios (OR) relating the prevalence of the thrombophilic condition in children with AIS to that in controls. The pooled OR (and 95% CI) were: protein C deficiency, 6.49 (2.96 to 14.27); protein S deficiency, 1.14 (0.34 to 3.80); AT deficiency, 1.02 (0.28 to 3.67); APCr, 1.34 (0.16 to 11.52); FV1691 GA, 1.22 (0.80 to 1.87); PT20210GA, 1.10 (0.51 to 2.34); MTHFR C677T, 1.70 (1.23 to 2.34); and total plasma homocysteine >95th centile, 1.36 (0.53 to 3.51). There was no statistical heterogeneity within these data. Conclusions: All factors examined were more common in children with first AIS than in controls, and significantly so for protein C deficiency and the MTHFR C677T mutation. The implications of thrombophilia for prognosis and recurrence need to be established before clinical recommendations can be made regarding investigation and treatment of children with AIS.
Interdecadal changes in intensity of the oxygen minimum zone off Concepción, Chile (~ 36° S), over the last century

Abstract. We reconstructed oxygenation changes in the upwelling ecosystem off Concepcion (36° S), Chile, using inorganic and organic proxies in a sediment core covering the last ca. 110 years of sedimentation in this area. Authigenic enrichments of Mo, U and Cd were observed between ca. 1935 and 1971 CE, implying a prolonged period with predominantly more reduced conditions in bottom waters and surface sediments. Significant positive correlations between redox-sensitive metals, algal sterols, biomarkers of micro-aerophilic and anaerobic microorganisms, and archaeal glycerol dialkyl glycerol tetraethers point to a tight coupling among bottom water O2 depletion and increased primary and export production. The time interval with low O2 of ca. 35 years seems to follow low-frequency interdecadal variation of the Pacific Decadal Oscillation, and it may have resulted in O2 depletion over the entire continental shelf off Concepcion. Taking this together with the concurrent increase in sedimentary molecular indicators of micro-aerophilic and anaerobic microbes, we can suggest that changes in oxygenation of the water column are reflected by changes in microbial community. This study can inform our understanding of ecological consequences to projected trends in ocean deoxygenation.
Table-top spatially-augmented realty: bringing physical models to life with projected imagery

Despite the availability of high-quality graphics systems, architects and designers still build scaled physical models of buildings and products. These physical models have many advantages, however they are typically static in structure and surface characteristics. They are inherently lifeless. In contrast, high-quality graphics systems are tremendously flexible, allowing viewers to see alternative structures, facades, textures, cut-away views, and even dynamic effects such as changing lighting, moving automobiles, people, etc. We introduce a combination of these approaches that builds on our previously-published projector-based spatially-augmented reality techniques. The basic idea is to aim multiple ceiling-mounted light projectors inward to graphically augment table-top scaled physical models of buildings or products. This approach promises to provide very compelling hybrid visualizations that afford the benefits of both traditional physical models, and modern computer graphics, effectively "bringing to life" table-top physical models.
Comparative Analysis of the Conventional and Novel pmo (Particulate Methane Monooxygenase) Operons from Methylocystis Strain SC2

ABSTRACT In addition to the conventional pmoA gene (pmoA1) encoding the active site polypeptide of particulate methane monooxygenase, a novel pmoA gene copy (pmoA2) is widely distributed among type II methanotrophs (methane-oxidizing bacteria [MOB]) (M. Tchawa Yimga, P. F. Dunfield, P. Ricke, J. Heyer, and W. Liesack, Appl. Environ. Microbiol. 69:5593-5602, 2003). Here we report that the pmoA1 and pmoA2 gene copies in the type II MOB Methylocystis strain SC2 are each part of a complete pmoCAB gene cluster (pmoCAB1, pmoCAB2). A bacterial artificial chromosome (BAC) library of strain SC2 genomic DNA was constructed, and BAC clones carrying either pmoCAB1 or pmoCAB2 were identified. Comparative sequence analysis showed that these two gene clusters exhibit low levels of identity at both the DNA level (67.4 to 70.9%) and the derived protein level (59.3 to 65.6%). In contrast, the secondary structures predicted for PmoCAB1 and PmoCAB2, as well as the derived transmembrane-spanning regions, are nearly identical. This suggests that PmoCAB2 is, like PmoCAB1, a highly hydrophobic, membrane-associated protein. A total of 190 of the 203 amino acid residues representing a highly conserved consensus sequence of the currently known PmoCAB1 and AmoCAB sequence types could be identified in PmoCAB2. The amoCAB gene cluster encodes ammonia monooxygenase and is evolutionarily related to pmoCAB. Analysis of a set of amino acid residues that allowed differentiation between conventional PmoA and AmoA provided further support for the hypothesis that pmoCAB2 encodes a functional equivalent of PmoCAB1. In experiments in which we used 5′ rapid amplification of cDNA ends we identified transcriptional start sites 320 and 177 bp upstream of pmoC1 and pmoC2, respectively. Immediately upstream of the transcriptional start sites of both pmoCAB1 and pmoCAB2, sequence motifs similar to Escherichia coli σ70 promoters were identified.
APRIL: a processor architecture for multiprocessing

The architecture of a rapid-context-switching processor called APRIL, with support for fine-grain threads and synchronization, is described. APRIL achieves high single-thread performance and supports virtual dynamic threads. A commercial reduced-instruction-set-computer-(RISC-) based implementation of APRIL and a run-time software system that can switch contexts in about 10 cycles are described. Measurements taken for several parallel applications on an APRIL simulator show that the overhead for supporting parallel tasks based on futures is reduced by a factor of 2 over a corresponding implementation on the Encore Multimax. The scalability of a multiprocessor based on APRIL is explored using a performance model. The authors show that the SPARC-based implementation of APRIL can achieve close to 80% processor utilization with as few as three resident threads per processor in a large-scale cache-based machine with an average base network latency of 55 cycles. <<ETX>>
Home parenteral nutrition in adults

Purpose of reviewThis review summarizes the latest epidemiologic data, and indications and methods for preventing complications from home parenteral nutrition (HPN), and it discusss survival, prognosis and quality of life of HPN patients who are potential candidates for intestinal transplantation. Recent findingsAlthough alteration in hepatic enzymes is frequent with HPN, severe HPN-related liver disorders are rare because of improvements in nutritional support. In experienced centres, likelihood of survival in HPN patients may reach 86% and 71% at 1 and 10 years, respectively. About 15% of adult HPN patients are potential candidates for intestinal transplantation. SummaryComplications include catheter-related sepsis and thrombosis, metabolic disorders, nutritional deficiency, psychological alteration and rehabilitation problems. Recent studies found that catheters lasted for 1.5 years on average. Alteration in hepatic enzymes alterations is frequent but severe liver complications are rare. Bone alterations occur in 75% of HPN patients, with multifactorial aetiology. Lipid administration should not exceed 1 g/kg per day. Overall probability of survival is good at 1 and 10 years; 10% of deaths are due to HPN complications. A large survey in European centres revealed that 15% of adults and 34% of children are candidates for intestinal transplantation.
Stool-fermented Plantago ovata husk induces apoptosis in colorectal cancer cells independently of molecular phenotype

Several studies have suggested that the partially fermentable fibre Plantago ovata husk (PO) may have a protective effect on colorectal cancer (CRC). We studied the potentially pro-apoptotic effect of PO and the implicated mechanisms in CRC cells with different molecular phenotypes (Caco-2, HCT116, LoVo, HT-29, SW480) after PO anaerobic fermentation with colonic bacteria as it occurs in the human colon. The fermentation products of PO induced apoptosis in all primary tumour and metastatic cell lines, independent of p53, adenomatous polyposis coli, β-catenin or cyclo-oxygenase-2 status. Apoptosis was caspase-dependent and both intrinsic and extrinsic pathways were implicated. The intrinsic pathway was activated through a shift in the balance towards a pro-apoptotic environment with an up-regulation of B-cell lymphoma protein 2 homologous antagonist killer (BAK) and a down-regulation of B-cell lymphoma-extra large (Bcl-xL) seen in HCT116 and LoVo cells. This resulted in mitochondrial membrane depolarisation, increased expression of caspase activators second mitochondria-derived activator of caspases (Smac)/Diablo, death effector apoptosis-inducing factor, apoptosome member apoptotic protease activating factor 1 and down-regulation of inhibitors of apoptosis Survivin and X-linked inhibitor of apoptosis in most cells. The extrinsic pathway was activated presumably through the up-regulation of death receptor (DR5). Some important differences were seen between primary tumour and metastatic CRC cells. Thus, metastatic PO-treated LoVo cells had a remarkable up-regulation of TNF-α ligand along with death-inducing signalling complex components receptor interacting protein and TNF-α receptor 1-associated death domain protein. The extrinsic pathway modulator FCICE-inhibitory protein (FLIP), an inhibitor of both spontaneous death ligand-independent and death receptor-mediated apoptosis, was significantly down-regulated after PO treatment in all primary tumour cells, but not in metastatic LoVo. These findings suggest that PO could potentially be a useful chemotherapy adjuvant.
Nonspecific lymphocyte responses in F344 and LEW rats: susceptibility to murine respiratory mycoplasmosis and examination of cellular basis for strain differences

Mycoplasma pulmonis produces a mitogen which may play a role in the pathogenesis of murine respiratory mycoplasmosis in rats. Since LEW rats are more susceptible to this disease than F344 rats are, these two strains were used to examine a possible association between disease severity and the level of nonspecific lymphocyte stimulation by mitogens, including M. pulmonis membrane preparations. F344 and LEW spleen, lung, blood, and lymph node lymphocytes were exposed to various mitogens. LEW lymphocytes gave a significantly higher response to mitogenic stimulation, regardless of their anatomical source. These differences in lymphocyte responsiveness were primarily due to differences within the nonadherent cell population. Significantly higher numbers of W3/25+ (T helper) cells were found in LEW lymphoid populations, whereas no difference was found in MRC OX-8+ (T suppressor/cytotoxic) cells. These data suggest an association between disease severity and host responsiveness to nonspecific stimuli.
Pain relief for strabismus surgery in children: a randomised controlled study of the use of preoperative sub-Tenon levobupivacaine

Aims: To compare the postoperative pain scores in children undergoing squint surgery administered with preoperative sub-Tenon levobupivacaine for postoperative pain relief versus controls. Methods: A prospective randomised controlled clinical trial was performed. Children aged 1–16 years undergoing strabismus surgery were recruited. The test group received sub-Tenon levobupivacaine preoperatively and topical anaesthetic eye-drops at the end of the procedure. The control group received topical anaesthetic eye-drops only at the end of surgery. Pain scores were recorded at 30 min, 2, 4, 6 and 24 h postoperatively using the Wong–Baker Pain or FLACC (face, legs, arms, cry, consolability) assessment score. The principal outcome measured was the pain score at each time interval for both groups. Results: 27 patients received sub-Tenon levobupivacaine, and there were 27 age- and sex-matched controls. The pain score in the test group was not lower than that of the control group (p = 0.22 at 30 min, p = 0.37 at 2 h). Conclusions: Sub-Tenon levobupivacaine, which is widely used for postoperative pain relief in paediatric strabismus surgery, was not effective when administered preoperatively in this cohort.
Recombinant myostatin (GDF-8) propeptide enhances the repair and regeneration of both muscle and bone in a model of deep penetrant musculoskeletal injury.

BACKGROUND Myostatin (GDF-8) is known as a potent inhibitor of muscle growth and development, and myostatin is also expressed early in the fracture healing process. The purpose of this study was to test the hypothesis that a new myostatin inhibitor, a recombinant myostatin propeptide, can enhance the repair and regeneration of both muscle and bone in cases of deep penetrant injury. METHODS We used a fibula osteotomy model with associated damage to lateral compartment muscles (fibularis longus and brevis) in mice to test the hypothesis that blocking active myostatin with systemic injections of a recombinant myostatin propeptide would improve muscle and bone repair. Mice were assigned to two treatment groups after undergoing a fibula osteotomy: those receiving either vehicle (saline) or recombinant myostatin propeptide (20 mg/kg). Mice received one injection on the day of surgery, another injection 5 days after surgery, and a third injection 10 days after surgery. Mice were killed 15 days after the osteotomy procedure. Bone repair was assessed using microcomputed tomography (micro-CT) and histologic evaluation of the fracture callus. Muscle healing was assessed using Masson trichrome staining of the injury site, and image analysis was used to quantify the degree of fibrosis and muscle regeneration. RESULTS Three propeptide injections over a period of 15 days increased body mass by 7% and increased muscle mass by almost 20% (p < 0.001). Micro-CT analysis of the osteotomy site shows that by 15 days postosteotomy, bony callus tissue was observed bridging the osteotomy gap in 80% of the propeptide-treated mice but only 40% of the control (vehicle)-treated mice (p < 0.01). Micro-CT quantification shows that bone volume of the fracture callus was increased by ∼ 30% (p < 0.05) with propeptide treatment, and the increase in bone volume was accompanied by a significant increase in cartilage area (p = 0.01). Propeptide treatment significantly decreased the fraction of fibrous tissue in the wound site and increased the fraction of muscle relative to fibrous tissue by 20% (p < 0.01). CONCLUSIONS Blocking myostatin signaling in the injured limb improves fracture healing and enhances muscle regeneration. These data suggest that myostatin inhibitors may be effective for improving wound repair in cases of orthopaedic trauma and extremity injury.