Miracle-dz/UniFAR-FTUs huggingface dataset:
Results of the Prospective, Randomized, Multicenter Food and Drug Administration Investigational Device Exemption Study of the ProDisc®-L Total Disc Replacement Versus Circumferential Fusion for the Treatment of 1-Level Degenerative Disc Disease

Study Design. A prospective, randomized, multicenter, Food and Drug Administration-regulated Investigational Device Exemption clinical trial. Objective. To evaluate the safety and effectiveness of the ProDisc®-L (Synthes Spine, West Chester, PA) lumbar total disc replacement compared to circumferential spinal fusion for the treatment of discogenic pain at 1 vertebral level between L3 and S1. Summary of Background Data. As part of the Investigational Device Exemption clinical trial, favorable single center results of lumbar total disc replacement with the ProDisc®-L have been reported previously. Methods. Two hundred eighty-six (286) patients were treated on protocol. Patients were evaluated before and after surgery, at 6 weeks, 3, 6, 12, 18, and 24 months. Evaluation at each visit included patient self-assessments, physical and neurologic examinations, and radiographic evaluation. Results. Safety of ProDisc®-L implantation was demonstrated with 0% major complications. At 24 months, 91.8% of investigational and 84.5% of control patients reported improvement in the Oswestry Low Back Pain Disability Questionnaire (Oswestry Disability Index [ODI]) from preoperative levels, and 77.2% of investigational and 64.8% of control patients met the ≥15% Oswestry Disability Index improvement criteria. Overall neurologic success in the investigational group was superior to the control group (91.2% investigational and 81.4% control; P = 0.0341). At 6 weeks and 3 months follow-up time points, the ProDisc®-L patients recorded SF-36 Health Survey scores significantly higher than the control group (P = 0.018, P = 0.0036, respectively). The visual analog scale pain assessment showed statistically significant improvement from preoperative levels regardless of treatment (P < 0.0001). Visual analog scale patient satisfaction at 24 months showed a statistically significant difference favoring investigational patients over the control group (P = 0.015). Radiographic range of motion was maintained within a normal functional range in 93.7% of investigational patients and averaged 7.7°. Conclusions. ProDisc®-L has been found to be safe and efficacious. In properly chosen patients, ProDisc®-L has been shown to be superior to circumferential fusion by multiple clinical criteria.
Indicators of Renal Glomerular and Tubular Functions in Patients with Beta-Thalassaemia Major: A cross sectional study at the Royal Hospital, Oman.

OBJECTIVES There are limited data concerning the assessment of renal function in beta-thalassaemia major, with no study of such involvement in Omani patients. The objective of this study was to establish the pattern of renal glomerular and tubular function using traditional and specific laboratory tests in patients with beta-thalassaemia major. METHODS This cross-sectional study, from January-July 2008, included 30 patients of the Thalassaemia Clinic at the Royal Hospital, Oman, with transfusion-dependent homozygous beta-thalassaemia major. They included 15 males and 15 females, aged 16-32 years with mean ± standard deviation of 21.23 ± 3.42 years. The medical records were reviewed and renal function states assessed as follows: serum creatinine, estimated glomerular filtration rate (eGFR); urea; phosphate, fractional excretion of filtered sodium (FENa); urine albumin: creatinine index; urine ß2-microglobulin:creatinine index; tubular reabsorption of phosphate (TRP), and tubular maximum phosphate reabsorption (TmP)/GFR. RESULTS All patients had eGFR >90 ml/min/1.73m(2); serum creatinine <90 μmol/L; serum urea <6.0 mmol/L, and urine albumin:creatinine <2.5 mg/mmol. Only 2 (6.7%) patients had FENa >1% and 3 (10.0%) patients had urine ß2-microglobulin: creatinine >22 μg/mmol. All patients had TRP >0.85, of whom seven (23.3%) patients had values within the range of 0.85-0.95 and 23 (76.7%) had values >0.95. Also, all patients had TmP/GFR >1.0 mmol/L, of whom only one (3.3%) patient had TmP/GFR of 1.0-1.5, and 29 (96.7%) patients had TmP/GFR >1.5 mmol/L. Finally, 24 (80%) patients had serum phosphate >1.4 mmol/L. Linear regression revealed a highly significant correlation between serum phosphate and TmP/GFR (r = 0.904, P < 0.001). CONCLUSION Renal function, glomerular and tubular, appears to be well preserved in beta-thalassaemia major. Almost all renal function indicators were within the recommended ranges. Raised TmP/GFR and TRP were noted in the majority of patients, reflecting an up-trend in serum phosphate and therefore increasing renal phosphate reabsorption.
DEVELOPMENTAL INTER‐RELATIONS BETWEEN EARLY MATERNAL DEPRESSION, CONTEXTUAL RISKS, AND INTERPERSONAL STRESS, AND THEIR EFFECT ON LATER CHILD COGNITIVE FUNCTIONING

Maternal depression and contextual risks (e.g. poverty) are known to impact children's cognitive and social functioning. However, few published studies have examined how stress in the social environment (i.e. interpersonal stress) might developmentally inter‐relate with maternal depression and contextual risks to negatively affect a child in these domains. This was the purpose of the current study.
Report on the High Energy Phenomena Sessions HE 2, HE 3.2-3.4: Neutrinos and Muons. Interactions, Particle Physics Aspects, Astro-Particle Physics and Cosmology

The results presented at the 28th International Cosmic Ray Conference on neutrino and muon physics are summarized. Neutrinos and muons provide a huge amount of information on particle interactions up to very high energies and on fundamental particle properties. Results on neutrino oscillations in the atmospheric and solar neutrino sectors are summarized. Oscillations are well established in both sectors, and a more precise determination of oscillation parameters is requested in the next future. Neutrino telescopes taking data and under construction presented numerous results. Neutrinos as probes of the Universe are hopefully going to open, together with gravitational waves, a new era for Astrophysics. Cosmology has entered the precision era and the Dark Matter quest is still an open problem. Direct and indirect searches are complementary approaches to the problem. The results presented at this conference confirm that Astroparticle Physics and, in particular, Neutrino Physics are leading fields in fundamental research.
Major capsid reinforcement by a minor protein in herpesviruses and phage

Herpes simplex type 1 virus (HSV-1) and bacteriophage λ capsids undergo considerable structural changes during self-assembly and DNA packaging. The initial steps of viral capsid self-assembly require weak, non-covalent interactions between the capsid subunits to ensure free energy minimization and error-free assembly. In the final stages of DNA packaging, however, the internal genome pressure dramatically increases, requiring significant capsid strength to withstand high internal genome pressures of tens of atmospheres. Our data reveal that the loosely formed capsid structure is reinforced post-assembly by the minor capsid protein UL25 in HSV-1 and gpD in bacteriophage λ. Using atomic force microscopy nano-indentation analysis, we show that the capsid becomes stiffer upon binding of UL25 and gpD due to increased structural stability. At the same time the force required to break the capsid increases by ∼70% for both herpes and phage. This demonstrates a universal and evolutionarily conserved function of the minor capsid protein: facilitating the retention of the pressurized viral genome in the capsid. Since all eight human herpesviruses have UL25 orthologs, this discovery offers new opportunities to interfere with herpes replication by disrupting the precise force balance between the encapsidated DNA and the capsid proteins crucial for viral replication.
The new NHGRI-EBI Catalog of published genome-wide association studies (GWAS Catalog)

The NHGRI-EBI GWAS Catalog has provided data from published genome-wide association studies since 2008. In 2015, the database was redesigned and relocated to EMBL-EBI. The new infrastructure includes a new graphical user interface (www.ebi.ac.uk/gwas/), ontology supported search functionality and an improved curation interface. These developments have improved the data release frequency by increasing automation of curation and providing scaling improvements. The range of available Catalog data has also been extended with structured ancestry and recruitment information added for all studies. The infrastructure improvements also support scaling for larger arrays, exome and sequencing studies, allowing the Catalog to adapt to the needs of evolving study design, genotyping technologies and user needs in the future.
Algorithm Developments for Discrete Adjoint Methods

This paper presents a number of algorithm developments for adjoint methods using the 'discrete' approach in which the discretisation of the non-linear equations is linearised and the resulting matrix is then transposed. With a new iterative procedure for solving the adjoint equations, exact numerical equivalence is maintained between the linear and adjoint discretisations. The incorporation of strong boundary conditions within the discrete approach is discussed, as well as a new application of adjoint methods to linear unsteady flow in turbomachinery.
Life events, first depression onset and the serotonin transporter gene

Background A relationship between the serotonin transporter gene, adverse events and onset of major depression has been reported. Aims To replicate a gene × environment interaction in a cohort with longitudinal data for life events, experience of depression, parental bonding and neuroticism. Method Atthe 25-year follow-up, genomic DNA was obtained from 127 cohort members (mean age 48 years) to determine the genotype of the serotonin transporter gene-linked promoter region (5-HTTLPR). Associations were investigated between the 5-HTTLPR genotype, positive and adverse life events and the gene × environment interaction, and also between the 5-HTTLPR genotype and risk factors for depression. Results No relationship was found between 5-HTTLPR genotype and either risk factors for depression or positive life events. Adverse life events had a significantly greater impact on the onset of depression for individuals with the s/s genotype. Conclusions The 5-HTTLPR genotype is a significant predictor of onset of major depression following multiple adverse events. This is one of the more robust findings concerning specific biological risk factors for depression.
Tidal modulation of ice shelf buttressing stresses

ABSTRACT Ocean tides influence the flow of marine-terminating glaciers. Observations indicate that the large fortnightly variations in ice flow at Rutford Ice Stream in West Antarctica originate in the floating ice shelf. We show that nonlinear variations in ice shelf buttressing driven by tides can produce such fortnightly variations in ice flow. These nonlinearities in the tidal modulation of buttressing stresses can be caused by asymmetries in the contact stress from migration of the grounding line and bathymetric pinning points beneath the ice shelf. Using a simple viscoelastic model, we demonstrate that a combination of buttressing and hydrostatic stress variations can explain a diverse range of tidal variations in ice shelf flow, including the period, phase and amplitude of flow variations observed at Rutford and Bindschadler Ice Streams.
On-line search in a simple polygon

We consider a number of search and exploration problems, from the perspective of robot navigation in a simple polygon. These problems are “on-line” in the sense that the robot does not have access to the map of the polygon; it must make decisions as it proceeds, based only on what it has seen so far. For the problem of exploring a simple rectilinear polygon (under the Lr norm), Deng, Kameda, and Papadimitriou give a 2-competitive deterministic algorithm; we present a randomized exploration algorithm which is 5/4-competitive. Using similar techniques, we are able to give an algorithm for searching an arbitrary, unknown rectilinear polygon. No constant competitive ratio is attainable in this case, but our algorithm is within a constant factor of optimal in the worst case; in a sense, it is a generalization of some of the strategies of Baeza-Yates, Culberson, and Rawlins to a much more general class of search spaces. Finally, we examine a type of polygon for which competitive search is possible the class of “streets” considered by Klein, who gave a 1 + $rcompetitive algorithm for the search problem in this case. We present a simple algorithm which is qcompetitive, improving this previous bound by more than a factor of 3.
Temperature e ff ects on gallium arsenide 63 Ni betavoltaic cell

A GaAs Ni radioisotope betavoltaic cell is reported over the temperature range 70 °C to −20 °C. The temperature effects on the key cell parameters were investigated. The saturation current decreased with decreased temperature; whilst the open circuit voltage, the short circuit current, the maximum power and the internal conversion efficiency values decreased with increased temperature. A maximum output power and an internal conversion efficiency of 1.8 pW (corresponding to 0.3 μW/Ci) and 7% were observed at −20 °C,
Viruses and asthma exacerbations

Key points Respiratory viral infections are the most common triggers of asthma attacks in both children and adults. Rhinoviruses are most frequently involved. Mechanisms include direct effects at the airway epithelium, as well as systemic responses. Currently available treatments are not very effective against virus-induced exacerbations. Educational aims To emphasise that respiratory viruses are major triggers of acute asthma exacerbations in both children and adults. To describe the current understanding of underlying mechanisms. To discuss possibilities for intervention. Summary Acute exacerbations of asthma are the major cause of morbidity and mortality of the disease, and one of the most difficult outcomes to prevent and treat. Respiratory viral infections cause >80% of asthma exacerbations in children and >50% in adults. In recent years, an increasing number of studies have investigated the mechanisms underlying asthma exacerbations; however, our understanding is still incomplete. Promising new data suggest the possibility for novel prevention and/or therapeutic strategies. This review aims to increase understanding of the epidemiology, mechanisms and potential treatments for virus-induced asthma exacerbations.
DIFFERENTIATION OF DORSAL-SPINED ELAPHOSTRONGYLINE LARVAE BY POLYMERASE CHAIN REACTION AMPLIFICATION OF ITS-2 of rDNA

Molecular genetics was used to devise the first reliable diagnostic tool for differentiating morphologically indistinguishable dorsal-spined, first-stage larvae (L1's) and other stages of the nematode protostrongylid subfamily Elaphostrongylinae. A polymerase chain reaction (PCR) assay employing specifically designed primers was developed to selectively amplify DNA of the ITS-2 region of the ribosomal gene. Amplification of the entire ITS-2 region differentiated between larvae of the genera Elaphostrongylus and Parelaphostrongylus, based on the lengths of fragments produced. Three sets of primers were designed and used successfully to distinguish larvae at the species level. Although it was demonstrated that one primer set in a single PCR assay was capable of distinguishing each of the three Parelaphostrongylus spp. , a second primer set would be required for confirmation in routine diagnostic use. Two of the three primer sets were capable of amplifying DNA from all six elaphostrongyline species and of identifying Elaphostrongylus alces and Parelaphostrongylus odocoilei. Although two separate fragments were produced from each Elaphostrongylus cervi and Elaphostrongylus rangiferi, it was not possible to distinguish these two parasites from each other based on the fragment size. The use of various nematodes, hosts, and fecal controls demonstrated the reliability of the primers for all developmental stages including L1's, third-stage larvae, and adult worms. These primers also have potential for identifying other lungworms as was shown by the amplification of Umingmakstrongylus pallikuukensis, the muskox protostrongylid, and Dictyocaulus sp. from white-tailed deer. Although this assay may benefit from further refinement, its present design provides researchers, wildlife managers, clinicians, and animal health regulators with a practical tool for the control, management, and study of meningeal and tissue worms and their close relatives.
Parafibromin, a Component of the Human PAF Complex, Regulates Growth Factors and Is Required for Embryonic Development and Survival in Adult Mice

ABSTRACT Parafibromin, a transcription factor associated with the PAF complex, is encoded by the HRPT2 gene, mutations of which cause the hyperparathyroidism-jaw tumor syndrome (OMIM145001). To elucidate the function of parafibromin, we generated conventional and conditional Hrpt2 knockout mice and found that Hrpt2−/− mice were embryonic lethal by embryonic day 6.5 (E6.5). Controlled deletion of Hrpt2 after E8.5 resulted in apoptosis and growth retardation. Deletion of Hrpt2 in adult mice led to severe cachexia and death within 20 days. To explore the mechanism underlying the embryonic lethality and death of adult mice, mouse embryonic fibroblasts (MEFs) were cultured and Hrpt2 was deleted in vitro. Hrpt2−/− MEFs underwent apoptosis, while Hrpt2+/+ and Hrpt2+/− MEFs grew normally. To study the mechanism of this apoptosis, Hrpt2+/+ and Hrpt2−/− MEFs were used in cDNA microarray, semiquantitative reverse transcription-PCR, and chromatin immunoprecipitation assays to identify genes regulated by parafibromin. These revealed that Hrpt2 expression and the parafibromin/PAF complex directly regulate genes involved in cell growth and survival, including H19, Igf1, Igf2, Igfbp4, Hmga1, Hmga2, and Hmgcs2. Thus, our results show that expression of Hrpt2 and parafibromin is pivotal in mammalian development and survival in adults and that these functions are likely mediated by the transcriptional regulation of growth factors.
Health Effects in Fish of Long-Term Exposure to Effluents from Wastewater Treatment Works

Concern has been raised in recent years that exposure to wastewater treatment effluents containing estrogenic chemicals can disrupt the endocrine functioning of riverine fish and cause permanent alterations in the structure and function of the reproductive system. Reproductive disorders may not necessarily arise as a result of estrogenic effects alone, and there is a need for a better understanding of the relative importance of endocrine disruption in relation to other forms of toxicity. Here, the integrated health effects of long-term effluent exposure are reported (reproductive, endocrine, immune, genotoxic, nephrotoxic). Early life-stage roach, Rutilus rutilus, were exposed for 300 days to treated wastewater effluent at concentrations of 0, 15.2, 34.8, and 78.7% (with dechlorinated tap water as diluent). Concentrations of treated effluents that induced feminization of male roach, measured as vitellogenin induction and histological alteration to gonads, also caused statistically significant alterations in kidney development (tubule diameter), modulated immune function (differential cell count, total number of thrombocytes), and caused genotoxic damage (micronucleus induction and single-strand breaks in gill and blood cells). Genotoxic and immunotoxic effects occurred at concentrations of wastewater effluent lower than those required to induce recognizable changes in the structure and function of the reproductive endocrine system. These findings emphasize the need for multiple biological end points in tests that assess the potential health effects of wastewater effluents. They also suggest that for some effluents, genotoxic and immune end points may be more sensitive than estrogenic (endocrine-mediated) end points as indicators of exposure in fish.
Erythropoietin as an angiogenic factor

Erythropoietin (Epo) is produced by the fetal liver and adult kidney and is an essential stimulator of erythropoiesis. It has, however, been shown to modulate host cellular signal transduction pathway to perform many other functions. New sites of Epo production have been found, such as the female reproductive organs and central nervous system. This review summarizes the involvement of Epo in the regulation of angiogenesis in both normal and pathological conditions.
SUBSTANCE ABUSE AND WITHDRAWAL : ALCOHOL , COCAINE , OPIOIDS AND OTHER DRUGS

Ethanol, illicit drugs, and prescription drugs used for nonmedical purposes are a significant medical as well as social problem. The 2005 National Survey on Drug Use and Health found that 22.7% of Americans, or 55 million individuals, were binge drinkers, which includes 16 million heavy drinkers (five or more drinks on the same occasion at least 5 different days in the prior 30 days) (1). Pain relievers were used nonmedically by 4.7 million Americans, and 3.5 million used stimulants or cocaine. Casual or habitual use of these drugs contributes to acute and chronic illness. Substance abuse also underlies many forms of injury, including vehicular accidents, falls, near-drowning, thermal injuries, homicide, and suicide. Other critical illnesses may be impacted by either substance use or substance withdrawal. This chapter will cover acute toxicity and withdrawal syndromes related to ethanol, cocaine, opioids, and other selected drugs likely to be of importance to the critical care practitioner.
Mapping HPV Vaccination and Cervical Cancer Screening Practice in the Pacific Region-Strengthening National and Regional Cervical Cancer Prevention.

OBJECTIVE To provide background information for strengthening cervical cancer prevention in the Pacific by mapping current human papillomavirus (HPV) vaccination and cervical cancer screening practices, as well as intent and barriers to the introduction and maintenance of national HPV vaccination programmes in the region. MATERIALS AND METHODS A cross-sectional questionnaire-based survey among ministry of health officials from 21 Pacific Island countries and territories (n=21). RESULTS Cervical cancer prevention was rated as highly important, but implementation of prevention programs were insufficient, with only two of 21 countries and territories having achieved coverage of cervical cancer screening above 40%. Ten of 21 countries and territories had included HPV vaccination in their immunization schedule, but only two countries reported coverage of HPV vaccination above 60% among the targeted population. Key barriers to the introduction and continuation of HPV vaccination were reported to be: (i) Lack of sustainable financing for HPV vaccine programs; (ii) Lack of visible government endorsement; (iii) Critical public perception of the value and safety of the HPV vaccine; and (iv) Lack of clear guidelines and policies for HPV vaccination. CONCLUSION Current practices to prevent cervical cancer in the Pacific Region do not match the high burden of disease from cervical cancer. A regional approach, including reducing vaccine prices by bulk purchase of vaccine, technical support for implementation of prevention programs, operational research and advocacy could strengthen political momentum for cervical cancer prevention and avoid risking the lives of many women in the Pacific.