Miracle-dz/UniFAR-FTUs huggingface dataset:
A negative arterial-portal venous glucose gradient decreases skeletal muscle glucose uptake.

The effect of a negative arterial-portal venous (a-pv) glucose gradient on skeletal muscle and whole body nonhepatic glucose uptake was studied in 12 42-h-fasted conscious dogs. Each study consisted of a 110-min equilibration period, a 30-min baseline period, and two 120-min hyperglycemic (2-fold basal) periods (either peripheral or intraportal glucose infusion). Somatostatin was infused along with insulin (3 x basal) and glucagon (basal). Catheters were inserted 17 days before studies in the external iliac artery and hepatic, portal and common iliac veins. Blood flow was measured in liver and hindlimb using Doppler flow probes. The arterial blood glucose, arterial plasma insulin, arterial plasma glucagon, and hindlimb glucose loads were similar during peripheral and intraportal glucose infusions. The a-pv glucose gradient (in mg/dl) was 5 +/- 1 during peripheral and -18 +/- 3 during intraportal glucose infusion. The net hindlimb glucose uptakes (in mg/min) were 5.0 +/- 1.2, 20.4 +/- 4.5, and 14.8 +/- 3.2 during baseline, peripheral, and intraportal glucose infusion periods, respectively (P < 0.01, peripheral vs. intraportal); the hindlimb glucose fractional extractions (in %) were 2.8 +/- 0.4, 4.7 +/- 0.8, and 3.9 +/- 0.5 during baseline, peripheral, and intraportal glucose infusions, respectively (P < 0. 05, peripheral vs. intraportal). The net whole body nonhepatic glucose uptakes (in mg . kg-1 . min-1) were 1.6 +/- 0.1, 7.9 +/- 1.3, and 5.4 +/- 1.1 during baseline, peripheral, and intraportal glucose infusion, respectively (P < 0.05, peripheral vs. intraportal). In the liver, net glucose uptake was 70% greater during intraportal than during peripheral glucose infusion (5.8 +/- 0.7 vs. 3.4 +/- 0.4 mg . kg-1 . min-1). In conclusion, despite comparable glucose loads and insulin levels, hindlimb and whole body net nonhepatic glucose uptake decreased significantly during portal venous glucose infusion, suggesting that a negative a-pv glucose gradient leads to an inhibitory signal in nonhepatic tissues, among which skeletal muscle appears to be the most important.
Influence of seasonal variation on kinetics of time temperature integrators for thermally processed milk

In the context of identifying intrinsic time temperature integrators (TTIs) for evaluating thermal processing of milk, the possible influence of seasonal variation in milk composition on the applicability of alkaline phosphatase (ALP), hydroxymethylfurfural (HMF), lactulose and furosine for process impact assessment was studied. Hereto inactivation and formation kinetics of these indicators were analysed in milk samples collected over a one year period. Based on previous research (isothermal and non-isothermal heating conditions) it was assumed that thermal inactivation of ALP followed first order kinetics, and formation of HMF, lactulose and furosine could be described by pseudo-zero order kinetics. This allowed the use of a simplified experimental design to obtain the kinetic parameters. Based on 90% joint confidence regions, kinetic parameter values differed significantly between different batches of milk for all four indicators. Additionally, variable ALP activities and HMF concentrations were observed in the raw milk samples. Nevertheless, the observed differences did not seem to restrict the applicability of the selected TTIs as illustrated by the construction of temperature time tolerance (TTT-) diagrams. Through these diagrams, use of the TTIs implied an uncertainty of approximately 2°C between equivalent processes.
Autism spectrum disorders and hyperactive/impulsive behaviors in Japanese patients with Prader–Willi syndrome: A comparison between maternal uniparental disomy and deletion cases

This study aims to compare maternal uniparental disomy 15 (mUPD) and a paternal deletion of 15q11‐13 (DEL) of Prader–Willi syndrome (PWS) in regard to autism spectrum disorders (ASD). Forty‐five Japanese individuals with PWS were recruited from a single recruitment center. The participants consisted of 22 children (aged from 6 to 12) and 23 adolescents (aged from 13 to 19). Six children and seven adolescents were confirmed as having mUPD. Sixteen children and 16 adolescents were confirmed as having DEL. Under blindness to the participants' genotypes, a single psychologist carried out behavioral and psychological assessments, including the Wechsler Intelligence Scales, Pervasive Developmental Disorders Autism Society Japan Rating Scale (PARS), and ADHD‐Rating Scale‐IV (ADHD‐RS‐IV). Two comparisons were made: one between mUPD and DEL children and another between mUPD and DEL adolescents. In children, no significant differences were found between mUPD and DEL participants in terms of autistic (PARS childhood, P = 0.657) and impulsive behaviors (ADHD‐RS‐IV hyperactive/impulsive, P = 0.275). In adolescents, mUPD patients showed significantly more autistic symptomatology (PARS adolescent, P = 0.027) and significantly more impulsive behavior (ADHD‐RS‐IV hyperactive/impulsive, P = 0.01) than DEL patients. Our findings about Japanese PWS patients were consistent with previous researches from western countries not focused on Asian patients, indicating that mUPD cases would be more prone to ASD than DEL cases, regardless of ethnoregional differences. In addition, our data suggested that the behavioral difference between mUPD and DEL cases in terms of autistic and impulsive symptoms tend to be unrecognizable in their childhood. © 2014 The Authors. American Journal of Medical Genetics Part A Published by Wiley Periodicals, Inc.
MR angiography of the lower extremities.

OBJECTIVE Current MRI technology and postprocessing tools have enabled 3D contrast-enhanced MR angiography (MRA) to evolve into a first-line noninvasive diagnostic tool to evaluate vascular disorders. CONCLUSION In this article, 3D MRA techniques, bolus timing issues, new IV contrast agents allowing a steady-state acquisition, principals of postprocessing, and unenhanced MRA techniques are reviewed and how to effectively use 3D gadolinium-enhanced MRA for peripheral arterial imaging is described.
Tagging Named Entities in 19th Century and Modern Finnish Newspaper Material with a Finnish Semantic Tagger

Named Entity Recognition (NER), search, classification and tagging of names and name like informational elements in texts, has become a standard information extraction procedure for textual data during the last two decades. NER has been applied to many types of texts and different types of entities: newspapers, fiction, historical records, persons, locations, chemical compounds, protein families, animals etc. In general a NER system’s performance is genre and domain dependent. Also used entity categories vary a lot (Nadeau and Sekine, 2007). The most general set of named entities is usually some version of three part categorization of locations, persons and corporations. In this
Automated coaching to help parents increase their children's brushing frequency: an exploratory trial.

UNLABELLED Advances in digital communication, such as the internet, now provide a cost effective channel to reach and help families struggling to establish good oral hygiene in their homes. This paper describes a novel internet based oral hygiene intervention whose design draws from advances in social cognitive models of behaviour change. Intervention components included role-modelling cartoons for children, a guide for parents on using rewards, a personalised plan with clear steps, tips to follow and a weekly 10-minute review of progress. OBJECTIVE To evaluate the efficacy of the online coaching programme; specifically we expected that those in the intervention group would brush their teeth more frequently during the intervention period than those in the control group. BASIC RESEARCH DESIGN An exploratory trial using a randomised controlled parallel approach. PARTICIPANTS Children aged 5 to 9 years from 44 families (23 control and 21 intervention). MAIN OUTCOME MEASURE An objective monitoring of tooth brushing. RESULTS In the 3-week intervention period, children from families assigned to the coaching programme brushed their teeth 38% more often than those in the control group. CONCLUSIONS The programme was effective in a number of respects. Opportunities for further research are discussed, including the need to create a more engaging system and so increase compliance.
$4 pi$NaI(Tl) Detector Array for In Vivo Neutron Activation Analysis

A 4pigamma-ray spectrometer has been set up at McMaster Accelerator Laboratory for in vivo neutron activation analysis. To achieve an ideal detection geometry eight long NaI(Tl) detectors with square cross sections were arrayed. A central square hole was left empty for sample access. The pulse processing system comprised a signal sum box, a spectroscopy amplifier and an ADC. Spectrum accumulation in coincidence or anticoincidence mode was also feasible by adding separate shaping and time pick-off electronics to each detector unit. The calibration result showed a fairly high efficiency, a peak efficiency of 0.50 at 1332 keV, due to a sufficient detector thickness of 10.2 cm and a length of 40.6 cm. The spectrometer was used for the aluminum analysis in hand phantoms as the first application. The Al sensitivity and the detection limit achieved for a hand dose of 20 mSv are 1,310 cps/mg, 0.24 mg, respectively. The detection limit has been improved by a factor of 2.5
An Action Research Case Study: A Sociocultural Perspective on Native American Students Learning Mathematics in a Public Elementary School Classroom

This dissertation is a qualitative study utilizing action research methods to develop a case study on the experience of urban public elementary school Native American students in collaborative mathematics activities. Data was collected with observations, mathematics assessments, and interviews to study how public school Native first graders experience collaborative mathematics activities when culturally modified with Indigenous ways of knowing and being? A major challenge in analyzing this work was finding a theoretical framework that could explain the experience of Native students in a multicultural public school. Sociocultural theory was selected because it operationalizes the key features of the study: Indigenous ways of knowing and being and the joint activity of learning mathematics in a public school classroom. The study suggests that Native students were heterogeneous learners and responded to cognitive pluralism, a variety of instruction, student practice
Basic issues on the processing of web queries

In this paper we study three basic and key issues related to Web query processing: load balance, broker behavior, and performance by individual index servers. Our study, while preliminary, does reveal interesting tradeoffs: (1) load unbalance at low query arrival rates can be controlled with a simple measure of randomizing the distribution of documents among the index servers, (2) the broker is not a bottleneck, and (3) disk utilization is higher than CPU utilization.
Human cytomegalovirus ie2 negatively regulates alpha gene expression via a short target sequence near the transcription start site

Repression of human cytomegalovirus alpha (immediate-early) gene expression is under the control of the viral ie2 gene. Here we show that ie2 negatively regulates gene expression directed by the strong cytomegalovirus enhancer via a specific 15-bp target sequence (which we term cis repression signal [crs]). This crs is located between -14 and +1 relative to the transcription start site and will function in an orientation-independent fashion, consistent with repression occurring at the transcriptional level. Repression is dominant over transactivation by ie1 gene products. The crs (5'-CGTTTAGTGAACCGT-3') does not contain previously recognized binding sites for cellular transcription factors, and a precise copy is not found elsewhere in the human cytomegalovirus genome. The position of the signal near the transcription start site appears to be important in function; addition of the crs near the transcription start site of a heterologous promoter, from the thymidine kinase gene of herpes simplex virus type 1, conferred cytomegalovirus ie2-dependent repression upon this promoter. Thus, we propose that an ie2 gene product or an induced cellular protein mediates repression by binding to crs. Negative regulation of alpha gene expression may be important during viral replication or latency.
Covariation representations for Hermitian Lévy process ensembles of free infinitely divisible distributions

It is known that the so-called Bercovici-Pata bijection can be explained in terms of certain Hermitian random matrix ensembles (M d ) d≥1 whose asymptotic spectral distributions are free infinitely divisible. We investigate Hermitian Levy processes with jumps of rank one associated to these random matrix ensembles introduced by Benaych-Georges and Cabanal-Duvillard. A sample path approximation by covariation processes for these matrix Levy processes is obtained. As a general result we prove that any d×d complex matrix subordinator with jumps of rank one is the quadratic variation of a $\mathbb{C}^d$-valued Levy process. In particular, we have the corresponding result for matrix subordinators with jumps of rank one associated to the random matrix ensembles (M d ) d≥1 .
Unsupervised Coreference Resolution by Utilizing the Most Informative Relations

In this paper we present a novel method for unsupervised coreference resolution. We introduce a precision-oriented inference method that scores a candidate entity of a mention based on the most informative mention pair relation between the given mention entity pair. We introduce an informativeness score for determining the most precise relation of a mention entity pair regarding the coreference decisions. The informativeness score is learned robustly during few iterations of the expectation maximization algorithm. The proposed unsupervised system outperforms existing unsupervised methods on all benchmark data sets.
Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1.

Using the two‐hybrid method, we isolated a Saccharomyces cerevisiae cDNA encoding a protein homologous to Schizosaccharomyces pombe protein Dis3sp, using as bait, human GTPase Ran. The DIS3 gene is essential for viability and complements S.pombe mutant dis3–54 which is defective in mitosis. Although Dis3sc has no homology to RanBP1, it bound directly to Ran and the S.cerevisiae Ran homologue Cnr1, but not to the S.cerevisiae RCC1 homologue Srm1. Upon binding to Ran with a 1:1 molar ratio, Dis3sc enhanced a nucleotide‐releasing activity of RCC1 on Ran. In the presence of Dis3sc, the K(m) of RCC1 on Ran decreased by half, while the kcat was unchanged. In vivo, Dis3sp was present as oligomers of M(r) 670–200 kDa as previously reported, and the 200 kDa oligomer of Dis3sp was found to include Spi1 and Pim1, the S.pombe homologues of Ran and RCC1, respectively. Although the biological function of the heterotrimeric oligomer consisting of Dis3, Spi1 and Pim1 is unknown, our results indicate that Dis3 is a component of the RCC1‐Ran pathway.
Cortical excitability in patients with focal epilepsy: a study with high frequency repetitive transcranial magnetic stimulation (rTMS)

Epileptogenesis involves an increase in excitatory synaptic strength in the brain in a manner similar to synaptic potentiation. In the present study we investigated the mechanisms of short-term synaptic potentiation in patients with focal epilepsy by using 5 Hz repetitive transcranial magnetic stimulation (rTMS), a non invasive neurophysiological technique able to investigate the mechanisms of synaptic plasticity in humans. Ten patients with focal idiopathic cortical epilepsy were studied. 5 Hz-rTMS (10 stimuli-trains, 120% of motor threshold, RMT) was delivered over the first dorsal interosseus (FDI) motor area of both (affected and unaffected) hemispheres. Changes in the motor evoked potential (MEP) size in the FDI muscle during the trains and the RMT were measured and compared between the hemispheres. 5 Hz-rTMS was also delivered in a group of healthy subjects over both hemispheres. 5 Hz-rTMS in patients elicited a reduced MEP facilitation compared to normal subjects. The reduced MEP amplitude was more evident in the affected hemisphere than in the unaffected hemisphere. RMT in the affected hemisphere was higher than in the unaffected hemisphere and in healthy subjects. Our findings showing a decreased response to 5 Hz-rTMS over the affected hemisphere, differently from the expected results suggest a reduced cortical excitability in epileptic patients. We hypothesize an altered balance between excitatory and inhibitory circuits in epileptic patients under chronic therapy.
Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence

Listeria monocytogenes is a Gram‐positive intracellular parasite and the causative organism of human listeriosis. In this article we demonstrate that L. monocytogenes encodes a functional member of the CodY family of global regulatory proteins that is responsive to both GTP and branched chain amino acids. By transcript analyses we identified the CodY regulon in L. monocytogenes and demonstrated that it comprises genes involved in amino acid metabolism, nitrogen assimilation as well as genes involved in sugar uptake and incorporation, indicating a role for CodY in L. monocytogenes in both carbon and nitrogen assimilation. A ΔrelA mutation reduced expression of the CodY regulon in early stationary phase and introduction of a ΔcodY mutation into a ΔrelA strain restored virulence. These data indicate that the avirulence of the ΔrelA mutant can in part be explained by the continued repression of the CodY regulon. The phenotypes of ΔrelA and ΔcodY mutants were studied in J774.A1 and Caco‐2 cells and the ΔrelA mutation shown to effect intracellular growth. These results provide the first direct evidence that the activity of a CodY‐type protein influences pathogenesis and provides new information on the physiological adaptation of L. monocytogenes to post‐exponential phase growth and virulence.
From snakes to stars: the statistics of collapsed objects – II. Testing a generic scaling ansatz for hierarchical clustering

We develop a diagrammatic technique to represent the multipoint probability density function of mass fluctuations in terms of the statistical properties of individual collapsed objects, and relate this to other statistical descriptors such as cumulants, cumulant correlators and factorial moments. We use this approach to establish key scaling relations describing various measurable statistical quantities if clustering follows a simple general scaling ansatz, as expected in hierarchical models. We test these detailed predictions against high-resolution numerical simulations. We show that, when appropriate variables are used, the count probability distribution function (CPDF) shows clear scaling properties in the non-linear regime. We also show that analytic predictions made using the scaling model for the behaviour of the void probability function (VPF) also match the simulations very well. We generalize the results for the CPDF to the two-point (bivariate) count probability distribution function (2CPDF), and show that its behaviour in the simulations is also well described by the theoretical model, as is the bivariate void probability function (2VPF). We explore the behaviour of the bias associated with collapsed objects in the limit of large separations, finding that it depends only on the intrinsic scaling parameter associated with collapsed objects, and that the bias for two different objects can be expressed as a product of the individual biases of the objects. Having thus established the validity of the scaling ansatz in various different contexts, we use its consequences to develop a novel technique for correcting finite-volume effects in the estimation of multipoint statistical quantities from observational data.
Automatic selection of optimal Savitzky-Golay filter parameters for Coronary Wave Intensity Analysis

Coronary Wave Intensity Analysis (cWIA) is a technique capable of separating the effects of proximal arterial haemodynamics from cardiac mechanics. The cWIA ability to establish a mechanistic link between coronary haemodynamics measurements and the underlying pathophysiology has been widely demonstrated. Moreover, the prognostic value of a cWIA-derived metric has been recently proved. However, the clinical application of cWIA has been hindered due to the strong dependence on the practitioners, mainly ascribable to the cWIA-derived indices sensitivity to the pre-processing parameters. Specifically, as recently demonstrated, the cWIA-derived metrics are strongly sensitive to the Savitzky-Golay (S-G) filter, typically used to smooth the acquired traces. This is mainly due to the inability of the S-G filter to deal with the different timescale features present in the measured waveforms. Therefore, we propose to apply an adaptive S-G algorithm that automatically selects pointwise the optimal filter parameters. The newly proposed algorithm accuracy is assessed against a cWIA gold standard, provided by a newly developed in-silico cWIA modelling framework, when physiological noise is added to the simulated traces. The adaptive S-G algorithm, when used to automatically select the polynomial degree of the S-G filter, provides satisfactory results with ≤ 10% error for all the metrics through all the levels of noise tested. Therefore, the newly proposed method makes cWIA fully automatic and independent from the practitioners, opening the possibility to multi-centre trials.
NleC, a Type III Secretion Protease, Compromises NF-κB Activation by Targeting p65/RelA

The NF-κB signaling pathway is central to the innate and adaptive immune responses. Upon their detection of pathogen-associated molecular patterns, Toll-like receptors on the cell surface initiate signal transduction and activate the NF-κB pathway, leading to the production of a wide array of inflammatory cytokines, in attempt to eradicate the invaders. As a countermeasure, pathogens have evolved ways to subvert and manipulate this system to their advantage. Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) are closely related bacteria responsible for major food-borne diseases worldwide. Via a needle-like protein complex called the type three secretion system (T3SS), these pathogens deliver virulence factors directly to host cells and modify cellular functions, including by suppressing the inflammatory response. Using gain- and loss-of-function screenings, we identified two bacterial effectors, NleC and NleE, that down-regulate the NF-κB signal upon being injected into a host cell via the T3SS. A recent report showed that NleE inhibits NF-κB activation, although an NleE-deficient pathogen was still immune-suppressive, indicating that other anti-inflammatory effectors are involved. In agreement, our present results showed that NleC was also required to inhibit inflammation. We found that NleC is a zinc protease that disrupts NF-κB activation by the direct cleavage of NF-κB's p65 subunit in the cytoplasm, thereby decreasing the available p65 and reducing the total nuclear entry of active p65. More importantly, we showed that a mutant EPEC/EHEC lacking both NleC and NleE (ΔnleC ΔnleE) caused greater inflammatory response than bacteria carrying ΔnleC or ΔnleE alone. This effect was similar to that of a T3SS-defective mutant. In conclusion, we found that NleC is an anti-inflammatory bacterial zinc protease, and that the cooperative function of NleE and NleC disrupts the NF-κB pathway and accounts for most of the immune suppression caused by EHEC/EPEC.