Miracle-dz/UniFAR-FTUs huggingface dataset:
The Landscape of Extreme Genomic Variation in the Highly Adaptable Atlantic Killifish

Understanding and predicting the fate of populations in changing environments require knowledge about the mechanisms that support phenotypic plasticity and the adaptive value and evolutionary fate of genetic variation within populations. Atlantic killifish (Fundulus heteroclitus) exhibit extensive phenotypic plasticity that supports large population sizes in highly fluctuating estuarine environments. Populations have also evolved diverse local adaptations. To yield insights into the genomic variation that supports their adaptability, we sequenced a reference genome and 48 additional whole genomes from a wild population. Evolution of genes associated with cell cycle regulation and apoptosis is accelerated along the killifish lineage, which is likely tied to adaptations for life in highly variable estuarine environments. Genome-wide standing genetic variation, including nucleotide diversity and copy number variation, is extremely high. The highest diversity genes are those associated with immune function and olfaction, whereas genes under greatest evolutionary constraint are those associated with neurological, developmental, and cytoskeletal functions. Reduced genetic variation is detected for tight junction proteins, which in killifish regulate paracellular permeability that supports their extreme physiological flexibility. Low-diversity genes engage in more regulatory interactions than high-diversity genes, consistent with the influence of pleiotropic constraint on molecular evolution. High genetic variation is crucial for continued persistence of species given the pace of contemporary environmental change. Killifish populations harbor among the highest levels of nucleotide diversity yet reported for a vertebrate species, and thus may serve as a useful model system for studying evolutionary potential in variable and changing environments.
Harnessing the Power of Film to Combat Mental Health Stigma. A University College London Psychiatry Society Event.

AIMS To conduct a pilot study on a motion picture based, anti-stigma programme entitled, "The Wounded Healer film" to measure if it is associated with any changes in stigma variables in healthcare students and if it encouraged care seeking in this group. BACKGROUND The 2008 Stigma Shout Survey of almost 4000 people using mental health services and carers revealed that healthcare professionals are a common source of stigma reported by people with mental illness. Psychological problems are common in medical students and doctors, however, thelevel of care seeking in this group is low. Fear of exposure to stigmatization is a crucial barrier to accessing and using mental health services. Recent research has revealed that film-based interventions can be used to challenge mental health stigma and encourage care seeking. METHODS We conducted a single-arm, pre-post comparison study on students who attended the Wounded Healer film at a University College London Psychiatry Society event (n=11). Before and after exposure to the film, students were asked to complete validated stigma scales that measured mental health-related knowledge, attitudes and behaviours. Responses were on a Likert scale and participants also provided free free-text comments which were subjected to thematic analyses. RESULTS There was a statistically significant difference in the mental health-related knowledge pre-intervention score compared to the post-intervention score (p=0.0322). All participants either strongly agreed or agreed with the post-evaluation statement, "I feel inspired to raise awareness of the importance of mental health and to take action to challenge stigma. " CONCLUSION This pilot study provides provisional support that The Wounded Healer film may be associated with reductions in stigma variables in healthcare students and encourage them to seek care for their own psychological problems. More robust research in this area is needed before we can scale up such an initiative.
Performance analysis of finite buffered multistage interconnection networks

The authors present a model which can accurately evaluate the performance of single-buffered and multibuffered MINs (multistage interconnection networks) with 2*2 switching elements (SESs). Earlier models were inaccurate because of the simplicity, or hard to expand for larger SEs or buffer sizes due to their complexity. The proposed model for single-buffered MINs has only three states, including the blocked state, while it is very accurate by realistically modeling the transactions between buffers in adjacent stages and network cycles. The model was expanded for multibuffered MIN, and it was found to be still very accurate. Comparisons with simulation and the YLL model (see H.Y. Yoon et al., 1990) revealed that the proposed models are consistently much more accurate irrespective of the size of the network, buffer, and traffic condition. The proposed model can be easily expanded for any other structures and operation conditions of MINs. <<ETX>>
Promises and Partnership

We examine experimentally the impact of communication on trust and cooperation. Our design admits observation of promises, lies, and beliefs. The evidence is consistent with people striving to live up to others' expectations so as to avoid guilt, as can be modeled using psychological game theory. When players exhibit such guilt aversion, communication may influence motivation and behavior by influencing beliefs about beliefs. Promises may enhance trustworthy behavior, which is what we observe. We argue that guilt aversion may be relevant for understanding strategic interaction in a variety of settings, and that it may shed light on the role of language, discussions, agreements, and social norms in these contexts. Copyright The Econometric Society 2006. (This abstract was borrowed from another version of this item.)
A consensus promoter sequence for Caulobacter crescentus genes involved in biosynthetic and housekeeping functions

Caulobacter crescentus differentiates prior to each cell division to form two different daughter cells: a monoflagellated swarmer cell and a nonmotile stalked cell. Thus, one might expect that developmentally expressed genes would be regulated by mechanisms different from those used to regulate the expression of the biosynthetic genes. To determine a consensus promoter sequence for genes involved in biosynthetic or housekeeping functions, DNA fragments containing the regulatory regions of the ilvD, ilvR, cysC, pleC, and fdxA genes were cloned. S1 nuclease protection mapping and primer extension techniques were used to identify the transcription initiation sites. Comparison of the regulatory regions of these genes with those of the published sequences of the ilvBN, rrnA, trpFBA, dnaA, dnaK, hemE, and rsaA genes has resulted in the identification of a putative promoter consensus sequence. The -35 region contains the sequence TTGACGS, which is similar to the Escherichia coli -35 region, while the -10 region, GCTANAWC, has a more balanced GC content than the corresponding region in E. coli. Oligonucleotide-directed site-specific mutagenesis of both the ilvBN and pleC promoters indicates that mutations that make a promoter more like the consensus result in increased promoter activity, while mutations decreasing similarity to the consensus result in decreased promoter activity.
Treatment of advanced gastrointestinal cancer with genetically modified autologous mesenchymal stem cells - TREAT-ME-1 - a phase I, first in human, first in class trial

Purpose This phase I, first in human, first in class clinical study aimed at evaluating the safety, tolerability and efficacy of treatment with genetically modified mesenchymal stromal cells (MSC) in combination with ganciclovir (GCV). MSC_apceth_101 are genetically modified autologous MSCs used as vehicles for a cell-based gene therapy in patients with advanced gastrointestinal adenocarcinoma. Experimental design The study design consisted of a dose-escalation 3 + 3 design. All patients (n = 6) were treated with up to three applications of MSC_apceth_101, followed by GCV infusions given on three consecutive days starting 48 hours after injection of MSC_apceth_101. Three of six patients received a total dose of 1.5 × 106 cells/kg. Two patients received three doses of 1 × 106 cells/kg, while one patient received only two doses of 1 × 106 cells/kg due to a SADR. Results Six patients received MSC_apceth_101. No IMP-related serious adverse events occurred. Adverse-events related to IMP-injection were increased creatinine, cough, fever, and night sweat. TNF, IL-6, IL-8, IL-10 and sE-Selectin, showed that repeated application is immunologically safe, but induces a switch of the functional properties of monocytes to an inflammatory phenotype. Treatment induced stable disease in 4/6 patients, and progressive disease in 2/6 patients. Conclusion Treatment with MSC_apceth_101 in combination with GCV demonstrated acceptable safety and tolerability in patients with advanced gastrointestinal adenocarcinoma.
Dominant Set Clustering and Pooling for Multi-View 3D Object Recognition

View based strategies for 3D object recognition have proven to be very successful. The state-of-the-art methods now achieve over 90% correct category level recognition performance on appearance images. We improve upon these methods by introducing a view clustering and pooling layer based on dominant sets. The key idea is to pool information from views which are similar and thus belong to the same cluster. The pooled feature vectors are then fed as inputs to the same layer, in a recurrent fashion. This recurrent clustering and pooling module, when inserted in an off-the-shelf pretrained CNN, boosts performance for multi-view 3D object recognition, achieving a new state of the art test set recognition accuracy of 93.8% on the ModelNet 40 database. We also explore a fast approximate learning strategy for our cluster-pooling CNN, which, while sacrificing end-to-end learning, greatly improves its training efficiency with only a slight reduction of recognition accuracy to 93.3%. Our implementation is available at this https URL.
Antecedents of Coordination Effectiveness of Software Developer Dyads From Interacting Teams: An Empirical Investigation

Among the numerous reasons for software project failure, coordination problems are especially salient. Prior studies on coordination in software development are confined to team internal coordination and do not explicitly differentiate team internal and external coordination processes. This study presents a research model to explain the antecedents of coordination effectiveness of software developer dyads from interacting teams. Dyads in this study refer to software developer pairs where each member comes from a different team. We explore the antecedents by integrating interpersonal and technology-based coordination. We test this model using data collected from 59 software developer Dyads from interacting teams as well as from software developer leaders. The results reveal that the implicit knowledge sharing has a significant positive impact on coordination effectiveness. The use of explicit knowledge sharing and coordination technology have no statistically significant impact on coordination effectiveness although the teams studied were working predominantly in a collocated mode. Mutual trust and project commitment have a significant impact on knowledge sharing with mutual trust directly affecting both implicit and explicit knowledge sharing. Project commitment also has a direct impact on explicit knowledge sharing and mutual trust, but it does not directly affect implicit knowledge sharing. Theoretical and practical contributions are discussed. Limitations of this study are identified. Future research directions are also presented.
Management of Newly Diagnosed Type 2 Diabetes Mellitus (T2DM) in Children and Adolescents

Over the past 3 decades, the prevalence of childhood obesity has increased dramatically in North America, ushering in a variety of health problems, including type 2 diabetes mellitus (T2DM), which previously was not typically seen until much later in life. The rapid emergence of childhood T2DM poses challenges to many physicians who find themselves generally ill-equipped to treat adult diseases encountered in children. This clinical practice guideline was developed to provide evidence-based recommendations on managing 10- to 18-year-old patients in whom T2DM has been diagnosed. The American Academy of Pediatrics (AAP) convened a Subcommittee on Management of T2DM in Children and Adolescents with the support of the American Diabetes Association, the Pediatric Endocrine Society, the American Academy of Family Physicians, and the Academy of Nutrition and Dietetics (formerly the American Dietetic Association). These groups collaborated to develop an evidence report that served as a major source of information for these practice guideline recommendations. The guideline emphasizes the use of management modalities that have been shown to affect clinical outcomes in this pediatric population. Recommendations are made for situations in which either insulin or metformin is the preferred first-line treatment of children and adolescents with T2DM. The recommendations suggest integrating lifestyle modifications (ie, diet and exercise) in concert with medication rather than as an isolated initial treatment approach. Guidelines for frequency of monitoring hemoglobin A1c (HbA1c) and finger-stick blood glucose (BG) concentrations are presented. Decisions were made on the basis of a systematic grading of the quality of evidence and strength of recommendation. The clinical practice guideline underwent peer review before it was approved by the AAP. This clinical practice guideline is not intended to replace clinical judgment or establish a protocol for the care of all children with T2DM, and its recommendations may not provide the only appropriate approach to the management of children with T2DM. Providers should consult experts trained in the care of children and adolescents with T2DM when treatment goals are not met or when therapy with insulin is initiated. The AAP acknowledges that some primary care clinicians may not be confident of their ability to successfully treat T2DM in a child because of the child’s age, coexisting conditions, and/or other concerns. At any point at which a clinician feels he or she is not adequately trained or is uncertain about treatment, a referral to a pediatric medical subspecialist should be made. If a diagnosis of T2DM is made by a pediatric medical subspecialist, the primary care clinician should develop a comanagement strategy with the subspecialist to ensure that the child continues to receive appropriate care consistent with a medical home model in which the pediatrician partners with parents to ensure that all health needs are met.
Addressing Stroke Signs and Symptoms Through Public Education: The Stroke Heroes Act FAST Campaign

Introduction In 2003, only 18% of Massachusetts adults were aware of all signs and symptoms of stroke, but 80% would call 9-1-1 if they thought someone was having a stroke or heart attack. Because early recognition leads to early treatment and improved clinical outcomes, increasing symptom recognition could have an impact on stroke survival and stroke patients' quality of life. Methods We conducted secondary research to identify messages with evidence-based effectiveness for communicating stroke signs and symptoms. From these results, a Stroke Heroes Act FAST animation was created and concept-tested. Non-Hispanic white and non-Hispanic black women aged 40 to 64 years received education on stroke signs and symptoms. Knowledge change about stroke signs and symptoms was calculated immediately following and 3 months after the education session. Results Using Stroke Heroes Act FAST educational materials that were developed, 72 women (mean age, 54 years; 15.5% were non-Hispanic blacks) received education about signs and symptoms of stroke and took the pretests and posttests to assess knowledge change. Immediately after the education session, significant increases were seen in the percentage of participants who recognized that facial droop (92% vs 99%, P = .02) and arm weakness or numbness (86% vs 97%, P = .004) were symptoms of stroke. Of the 65 participants who were given the 3-month follow-up survey, 100% remembered slurred speech and facial drooping as symptoms; 98.5% recalled arm weakness or numbness; and 97% would call 9-1-1 if they thought someone was having a stroke. None of these is a significant change from the posttest. Conclusion The Stroke Heroes Act FAST kit may be a useful tool for improving knowledge of stroke signs and symptoms among adults.
Ecological drift and the distribution of species diversity

Ecological drift causes species abundances to fluctuate randomly, lowering diversity within communities and increasing differences among otherwise equivalent communities. Despite broad interest in ecological drift, ecologists have little experimental evidence of its consequences in nature, where competitive forces modulate species abundances. We manipulated drift by imposing 40-fold variation in the size of experimentally assembled annual plant communities and holding their edge-to-interior ratios comparable. Drift over three generations was greater than predicted by neutral models, causing high extinction rates and fast divergence in composition among smaller communities. Competitive asymmetries drove populations of most species to small enough sizes that demographic stochasticity could markedly influence dynamics, increasing the importance of drift in communities. The strong effects of drift occurred despite stabilizing niche differences, which cause species to have greater population growth rates when at low local abundance. Overall, the importance of ecological drift appears greater in non-neutral communities than previously recognized, and varies with community size and the type and strength of density dependence.
Detecting short spatial scale local adaptation and epistatic selection in climate‐related candidate genes in European beech (Fagus sylvatica) populations

Detecting signatures of selection in tree populations threatened by climate change is currently a major research priority. Here, we investigated the signature of local adaptation over a short spatial scale using 96 European beech (Fagus sylvatica L.) individuals originating from two pairs of populations on the northern and southern slopes of Mont Ventoux (south‐eastern France). We performed both single and multilocus analysis of selection based on 53 climate‐related candidate genes containing 546 SNPs. FST outlier methods at the SNP level revealed a weak signal of selection, with three marginally significant outliers in the northern populations. At the gene level, considering haplotypes as alleles, two additional marginally significant outliers were detected, one on each slope. To account for the uncertainty of haplotype inference, we averaged the Bayes factors over many possible phase reconstructions. Epistatic selection offers a realistic multilocus model of selection in natural populations. Here, we used a test suggested by Ohta based on the decomposition of the variance of linkage disequilibrium. Overall populations, 0.23% of the SNP pairs (haplotypes) showed evidence of epistatic selection, with nearly 80% of them being within genes. One of the between gene epistatic selection signals arose between an FST outlier and a nonsynonymous mutation in a drought response gene. Additionally, we identified haplotypes containing selectively advantageous allele combinations which were unique to high or low elevations and northern or southern populations. Several haplotypes contained nonsynonymous mutations situated in genes with known functional importance for adaptation to climatic factors.
Psychological Issues in Cosmetic Surgery: A Functional Overview

&NA; The relevance of psychological issues in cosmetic surgery has been well established in the literature. Early articles considered psychopathology to be prevalent in the plastic surgery population, whereas more recent views consider the typical cosmetic surgery candidate to be more psychologically stable. Despite these changes, the potential for psychopathology continues to be recognized. Consequently, screening for psychopathology during the preoperative interview has remained essential. To facilitate a working knowledge of psychological issues that may be useful in preoperative screening, this article reviews the current literature on the psychology of plastic surgery. In addition to discussing the psychological impact of cosmetic surgery, patient's motivations and expectations for surgery are discussed in the context of self‐image. After reviewing potential types of psychopathology, various preoperative screening techniques are discussed. Hasan JS. Psychological issues in cosmetic surgery: a functional overview. Ann Plast Surg 2000;44:89‐96
Enhanced slow wave sleep and improved sleep maintenance after gaboxadol administration during seven nights of exposure to a traffic noise model of transient insomnia

Slow wave sleep (SWS) has been reported to correlate with sleep maintenance, but whether pharmacological enhancement of SWS also leads to improved sleep maintenance is not known. Here we evaluate the time-course of the effects of gaboxadol, an extra-synaptic gamma-aminobutyric acid (GABA) agonist, on SWS, sleep maintenance, and other sleep measures in a traffic noise model of transient insomnia. After a placebo run-in, 101 healthy subjects (20–78 y) were randomized to gaboxadol (n = 50; 15 mg in subjects <65 y and 10 mg in subjects ≥65 y) or placebo (n = 51) for 7 nights (N1–N7). The model caused some disruption of sleep initiation and maintenance, with greatest effects on N1. Compared with placebo, gaboxadol increased SWS and slow wave activity throughout N1 to N7 (p < 0.05). Gaboxadol reduced latency to persistent sleep overall (N1–N7) by 4.5 min and on N1 by 11 min (both p < 0.05). Gaboxadol increased total sleep time (TST) overall by 16 min (p < 0.001) and on N1 by 38 min (p < 0.0001). Under gaboxadol, wakefulness after sleep onset was reduced by 11 min overall (p < 0.01) and by 29 min on N1 (p < 0.0001), and poly-somnographic awakenings were reduced on N1 (p < 0.05). Gaboxadol reduced self-reported sleep onset latency overall and on N1 (both p < 0.05) and increased self-reported TST overall (p < 0.05) and on N1 (p < 0.01). Subjective sleep quality improved overall (p < 0.01) and on N1 (p < 0.0001). Increases in SWS correlated with objective and subjective measures of sleep maintenance and subjective sleep quality under placebo and gaboxadol (p < 0.05). Gaboxadol enhanced SWS and reduced the disruptive effects of noise on sleep initiation and maintenance.