Miracle-dz/UniFAR-FTUs huggingface dataset:
Malaria Morbidity in High and Seasonal Malaria Transmission Area of Burkina Faso

Background Malariometric parameters are often primary endpoints of efficacy trials of malaria vaccine candidates. This study aims to describe the epidemiology of malaria prior to the conduct of a series of drug and vaccine trials in a rural area of Burkina Faso. Methods Malaria incidence was prospectively evaluated over one year follow-up among two cohorts of children aged 0–5 years living in the Saponé health district. The parents of 1089 children comprising a passive case detection cohort were encouraged to seek care from the local health clinic at any time their child felt sick. Among this cohort, 555 children were randomly selected for inclusion in an active surveillance sub-cohort evaluated for clinical malaria during twice weekly home visits. Malaria prevalence was evaluated by cross-sectional survey during the low and high transmission seasons. Results Number of episodes per child ranged from 0 to 6 per year. Cumulative incidence was 67.4% in the passive and 86.2% in the active cohort and was highest among children 0–1 years. Clinical malaria prevalence was 9.8% in the low and 13.0% in the high season (p>0.05). Median days to first malaria episode ranged from 187 (95% CI 180–193) among children 0–1 years to 228 (95% CI 212, 242) among children 4–5 years. The alternative parasite thresholds for the malaria case definition that achieved optimal sensitivity and specificity (70–80%) were 3150 parasites/µl in the high and 1350 parasites/µl in the low season. Conclusion Clinical malaria burden was highest among the youngest age group children, who may represent the most appropriate target population for malaria vaccine candidate development. The pyrogenic threshold of parasitaemia varied markedly by season, suggesting a value for alternative parasitaemia levels in the malaria case defintion. Regional epidemiology of malaria described, Sapone area field centers are positioned for future conduct of malaria vaccine trials.
Atypical Mycobacterial Infection Presenting as Persistent Skin Lesion in a Patient with Ulcerative Colitis

Immunosuppressive drugs are commonly used for the treatment of inflammatory bowel disease. Patients receiving immunosuppressants are susceptible to a variety of infections with opportunistic pathogens. We present a case of skin infection with Mycobacterium chelonae in a 60-year-old Caucasian woman with ulcerative colitis who had been treated with corticosteroids and azathioprine. The disease manifested with fever and rash involving the right leg. Infliximab was administered due to a presumptive diagnosis of pyoderma gangrenosum, leading to worsening of the clinical syndrome and admission to our hospital. Routine cultures from various sites were all negative. However, Ziehl-Neelsen staining of pus from the lesions revealed acid-fast bacilli, and culture yielded a rapidly growing mycobacterium further identified as M. chelonae. The patient responded to a clarithromycin-based regimen. Clinicians should be aware of skin lesions caused by atypical mycobacteria in immunocompromised patients with inflammatory bowel disease. Furthermore, they should be able to thoroughly investigate and promptly treat these conditions.
Pilocarpine-Induced Seizures Cause Selective Time-Dependent Changes to Adult-Generated Hippocampal Dentate Granule Cells

Aberrantly interconnected granule cells are characteristic of temporal lobe epilepsy. By reducing network stability, these abnormal neurons may contribute directly to disease development. Only subsets of granule cells, however, exhibit abnormalities. Why this is the case is not known. Ongoing neurogenesis in the adult hippocampus may provide an explanation. Newly generated granule cells may be uniquely vulnerable to environmental disruptions relative to their mature neighbors. Here, we determine whether there is a critical period after neuronal birth during which neuronal integration can be disrupted by an epileptogenic insult. By bromodeoxyuridine birthdating cells in green fluorescent protein-expressing transgenic mice, we were able to noninvasively label granule cells born 8 weeks before (mature), 1 week before (immature), or 3 weeks after (newborn) pilocarpine-epileptogenesis. Neuronal morphology was examined 4 and 8 weeks after pilocarpine treatment. Strikingly, almost 50% of immature granule cells exposed to pilocarpine-epileptogenesis exhibited aberrant hilar basal dendrites. In contrast, only 9% of mature granule cells exposed to the identical insult possessed basal dendrites. Moreover, newborn cells were even more severely impacted than immature cells, with 40% exhibiting basal dendrites and an additional 20% exhibiting migration defects. In comparison, <5% of neurons from normal animals exhibited either abnormality, regardless of age. Together, these data demonstrate the existence of a critical period after the birth of adult-generated neurons during which they are vulnerable to being recruited into epileptogenic neuronal circuits. Pathological brain states therefore may pose a significant hurdle for the appropriate integration of newly born endogenous, and exogenous, neurons.
Active Regression with Adaptive Huber Loss

This paper addresses the scalar regression problem through a novel solution to exactly optimize the Huber loss in a general semi-supervised setting, which combines multi-view learning and manifold regularization. We propose a principled algorithm to 1) avoid computationally expensive iterative schemes while 2) adapting the Huber loss threshold in a data-driven fashion and 3) actively balancing the use of labelled data to remove noisy or inconsistent annotations at the training stage. In a wide experimental evaluation, dealing with diverse applications, we assess the superiority of our paradigm which is able to combine robustness towards noise with both strong performance and low computational cost.
Global seasonal variations of midday planetary boundary layer depth from CALIPSO space‐borne LIDAR

We present a new global analysis of the depth of the planetary boundary layer (PBL) and consider regional variations throughout the year. PBL depth is estimated from the vertical variance of CALIPSO space‐borne LIDAR backscatter associated with aerosol and shallow clouds during midday satellite overpasses and is only retrieved in the absence of optically thick clouds. The resulting analysis of over 100 million retrievals per year is therefore only a sample with higher frequency over deserts and other regions of strong subsidence, and lower frequency over regions of deep convection such as the ITCZ, tropical rainforests, and the Asian Monsoon. The mean of sampled PBL depths ranges from 500 m over cold oceans to more than 3000 m over hot deserts. The seasonal cycle of analyzed PBL depth is stronger over land than over water, and seasonality over land and midlatitude oceans is of opposite sign. Wintertime storm tracks and stratocumulus regions over subtropical oceans are prominent features of the analysis. Although evaluation of the new analysis is difficult due to previous sparse sampling by other methods, comparison of LIDAR‐retrieved PBL depth with data collected by commercial aircraft generally shows good agreement.
90-kDa Heat Shock Protein Inhibition Abrogates the Topoisomerase I Poison-Induced G2/M Checkpoint in p53-Null Tumor Cells by Depleting Chk1 and Wee1

The G2/M cell cycle checkpoint is regulated by a multitude of signaling pathways after genotoxic stress. Herein, we report that treatment with the 90-kDa heat shock protein (Hsp90) molecular chaperone inhibitor 17-allylamino-17-demethoxygeldanamycin (17AAG) selectively abrogates the G2/M checkpoint induced by 7-ethyl-10-hydroxycamptothecin (SN-38), an active metabolite of irinotecan, in p53-null compared with p53-intact HCT116 colon cancer cells. The basis for this selectivity can be explained in part by the lack of p21 induction in p53-null cells. In accord with published results, we could show that treatment with 17AAG resulted in depletion of Chk1, a known Hsp90 client protein. In addition, we observed a time- and dose-dependent decrease in Wee1 kinase level, a negative regulator of mitosis, after 17AAG treatment in gastrointestinal cancer cells. Depletion of Wee1 protein preceded mitotic entry induced by 17AAG, and this decrease could be partially rescued by cotreatment with a proteasome inhibitor. Coimmunoprecipitation experiments showed that Hsp90 and Wee1 interacted in whole cells, and 17AAG treatment decreased the degradative half-life of Wee1, indicating that Wee1 is another Hsp90 client in mammalian cells. Knockdown of Chk1 and Wee1 by short interfering RNA each resulted in abrogation of the G2/M checkpoint induced by SN-38. The combination of SN-38 and 17AAG was shown to be synergistic in p53-null but not in parental HCT116 cells by median effect/combination index analysis. Taken together, 17AAG specifically inhibits the G2/M checkpoint in p53-defective cells by down-regulation of two critical checkpoint kinases, Chk1 and Wee1.
Selenium Supranutrition: Are the Potential Benefits of Chemoprevention Outweighed by the Promotion of Diabetes and Insulin Resistance?

Selenium was considered a toxin until 1957, when this mineral was shown to be essential in the prevention of necrotic liver damage in rats. The hypothesis of selenium chemoprevention is principally formulated by the observations that cancer incidence is inversely associated with selenium status. However, recent clinical and epidemiological studies demonstrate a role for some selenoproteins in exacerbating or promoting other disease states, specifically type 2 diabetes, although other data support a role of selenium in stimulating insulin sensitivity. Therefore, it is clear that our understanding in the role of selenium in glucose metabolism and chemoprevention is inadequate and incomplete. Research exploring the role of selenium in individual healthcare is of upmost importance and possibly will help explain how selenium is a double-edged sword in the pathologies of chronic diseases.
Multiple-Unit Holdings Yield Attenuated Endowment Effects

Previous endowment effect experiments have examined circumstances in which people encounter a single unit of a good e.g., one chocolate. We contrast single-unit treatments with multiple-unit treatments in which participants encounter several units of a good e.g., five chocolates. We observe endowment effects of typical magnitude for singleton holdings but attenuated endowment effects for multiple-unit holdings. Moreover, endowment effects consistently arise for singletons even as the definition of a unit is altered. For instance, participants holding one piece of chocolate show an endowment effect of standard size, but so do participants holding one box of chocolates. Yet the box contains about 20 individual pieces of chocolate, and participants given that many separate pieces show a substantially attenuated endowment effect. We thus propose the property of “unit dependence”: the definition of a unit can change, but contingent on any given definition, a pronounced endowment effect may emerge for singletons but not multiples. This paper was accepted by Teck Ho, decision analysis.
Reverse Group Consensus of Multi-Agent Systems in the Cooperation-Competition Network

In this paper, a reverse group consensus problem is investigated for the dynamic agents with the inputs in the cooperation-competition network which can be divided into two sub-networks. The weights between the agents in the same sub-network are positive, while the weights between the agents among different sub-networks are negative. Then, the reverse group consensus is firstly studied without the in-degree balance condition. By defining the mirror graph and establishing the solution of the multi-agent system, it is found that the reverse group consensus problem can be achieved if the mirror graph is strongly connected. The explicit expression of the error level is also derived, which would be vanished for multi-agent systems with some special kinds of inputs. Furthermore, as an extension, the decomposing of the cooperation-competition network is discussed, where the concept of the condensation undirected graph and the path balance condition are defined, and several effective sufficient conditions are obtained. Finally, numerical simulation demonstrates the effectiveness of the theoretical analysis.
CHILDREN'S PERCEPTIONS OF DEATH: A PIAGETIAN PERSPECTIVE

This study explored children’s perceptions of death from a Piagetian perspective. While a few international studies exist which have explored this issue, it was deemed useful to evaluate the perceptions of South African children. It seems that many researchers have not taken into account the importance of broader contextual experience in children’s perceptions of death, and it was felt that the current high death rate in South Africa may impact on both or either the rate and level at which children start to engage with and understand the concept of death. The participants of the current study were between the ages of five and twelve years, and were drawn from a Gauteng Place of Safety and a preschool. Individual, structured interviews were conducted on this cross-sectional population. The interviews contained questions adapted from Koocher’s (1973) research in this area, and responses were analysed by means of a traditional content analysis. It was found that children had a basic understanding of death concepts by the age of approximately seven years old. It was also found that the youngest age group of children possess an awareness of death, but tend to view it as reversible and not final. An understanding of the finality of death emerges around the age of seven. In general, the participant’s responses showed a close relationship between Piaget’s stages of cognitive development and how the young child reasons about death.
Impulse Response Model and Parameters for Indoor Channel Modeling at 60GHz

In order to realize very high throughput wireless local area network (WLAN) systems, which demand multi Gbps capacities/bitrates, the IEEE802.11. TGad (Next generation WLAN standard at 60GHz band) is in the standardization process. To evaluate and select the proposed physical layer modulation and coding schemes in the standardization process, channel models for each specified usage model are required. In this paper, suitable impulse channel models for that purpose are proposed. Propagation measurements are carried out in a living room environment in the 60GHz band. To cover various usage cases, line-of-sight (LOS) and non-line-of-site (NLOS) scenarios and three different signal transmission polarization types are examined, and each channel model's parameters are extracted by statistical analysis. These channel models are useful in designing systems for millimeter-wave wireless communications.
Glucose and lactate metabolism by Actinomyces naeslundii.

Actinomyces are among the predominant bacteria in the oral microflora. This review discusses the glucose and lactate metabolism of Actinomyces naeslundii and its ecological significance in dental plaque. This bacterium has the Embden-Meyerhof-Parnas (EMP) pathway as the main route to degrade glucose. The EMP pathway-derived metabolic intermediates, phosphoenolpyruvate (PEP) and pyruvate, are further converted into different end-products, depending on the environment. Under anaerobic conditions in the absence of bicarbonate, the pyruvate is converted into lactate by a lactate dehydrogenase. In the presence of bicarbonate, the PEP is combined with bicarbonate and then converted into succinate through the succinate pathway, while the pyruvate is converted into formate and acetate through the pyruvate formate-lyase pathway. Under aerobic conditions, the pyruvate liberates acetate and CO2 through a pathway initiated by a pyruvate dehydrogenase. A. naeslundii strains also degrade lactate, aerobically, to acetate and CO2 through the conversion of lactate into pyruvate by a NAD-independent lactate dehydrogenase. These strains also synthesize glycogen from a glycolytic intermediate, glucose 6-phosphate. Besides atmospheric conditions and bicarbonate, the intracellular reduction-oxidation potential, carbohydrate concentration, and environmental pH also modulate the metabolism of A. naeslundii. Some of the phosphorylating enzymes involved in A. naeslundii metabolism--e.g., GTP/polyphosphate (PPn)-dependent glucokinase, pyrophosphate (PPi)-dependent phosphofructokinase, UDP-glucose pyrophosphorylase, and GDP/IDP-dependent PEP carboxykinase--are unique to A. naeslundii and have not been found in other oral bacteria. The utilization of PPn and PPi as phosphoryl donors, together with glycogen synthesis and lactate utilization, could contribute to the efficient energy metabolism found in A. naeslundii. Through this flexible and efficient metabolic capacity, A. naeslundii can adapt to fluctuating environments and compete with other bacteria in dental plaque. Further, this bacterium may modify the dental plaque environment and promote the microbial population shifts in dental plaque.
[Construction of recombinant GST-RCAS1 fusion gene and its expression in E. Coli].

OBJECTIVE To construct the recombinant plasmid of RCAS1, to express and purify its fusion protein GST-RCAS1, and to investigate its biological function. METHODS RCAS1 encoding gene was amplified by RT-PCR from total RNA extract of MCF-7 cells and was ligated with expression plasmid vector pGEX-2T by T4 DNA ligase after digested by the restricted endonucleases BamH I and EcoR I. Then the ligated products were inserted into competence JM109 E. Coli and the positive recombinants were identified by restriction endonuclease digestion assay and DNA sequencing. The GST-RCAS1 fusion protein expression was induced by IPTG in BL21 E. Coli and was purified with GST column and identified by SDS-PAGE and Western blotting with anti-GST monoclonal antibody, anti-RCAS1 (N-18) and anti-RCAS1 (C-20) polyclonal antibody. The apoptosis of activated T cells induced by GST-RCAS1 fusion protein was detected by flow cytometry with Annexin V and propidium iodide (PI) staining. RESULT A 642 bp product was cloned by RT-PCR and the recombinant plasmid was constructed successfully. The GST-RCAS1 fusion protein was recognized by GST monoclonal antibody and RCAS1(N-18 and C-20) polyclonal antibody. FACS analysis showed that GST-RCAS1 fusion protein induced apoptosis in activated T cells. CONCLUSION The recombinant plasmid of RCAS1 has been successfully constructed and the GST-RCAS1 fusion protein expressed and purified. The apoptosis inducing effect of GST-RCAS1 fusion protein on activated T cells is demonstrated.
Risk Factors for Hospital Admission with RSV Bronchiolitis in England: A Population-Based Birth Cohort Study

Objective To examine the timing and duration of RSV bronchiolitis hospital admission among term and preterm infants in England and to identify risk factors for bronchiolitis admission. Design A population-based birth cohort with follow-up to age 1 year, using the Hospital Episode Statistics database. Setting 71 hospitals across England. Participants We identified 296618 individual birth records from 2007/08 and linked to subsequent hospital admission records during the first year of life. Results In our cohort there were 7189 hospital admissions with a diagnosis of bronchiolitis, 24.2 admissions per 1000 infants under 1 year (95%CI 23.7–24.8), of which 15% (1050/7189) were born preterm (47.3 bronchiolitis admissions per 1000 preterm infants (95% CI 44.4–50.2)). The peak age group for bronchiolitis admissions was infants aged 1 month and the median was age 120 days (IQR = 61–209 days). The median length of stay was 1 day (IQR = 0–3). The relative risk (RR) of a bronchiolitis admission was higher among infants with known risk factors for severe RSV infection, including those born preterm (RR = 1.9, 95% CI 1.8–2.0) compared with infants born at term. Other conditions also significantly increased risk of bronchiolitis admission, including Down's syndrome (RR = 2.5, 95% CI 1.7–3.7) and cerebral palsy (RR = 2.4, 95% CI 1.5–4.0). Conclusions Most (85%) of the infants who are admitted to hospital with bronchiolitis in England are born at term, with no known predisposing risk factors for severe RSV infection, although risk of admission is higher in known risk groups. The early age of bronchiolitis admissions has important implications for the potential impact and timing of future active and passive immunisations. More research is needed to explain why babies born with Down's syndrome and cerebral palsy are also at higher risk of hospital admission with RSV bronchiolitis.
Clinical ocular findings in cows with malignant catarrhal fever: ocular disease progression and outcome in 25 cases (2007-2010).

OBJECTIVE To describe the ocular clinical signs in a group of cows diagnosed with sheep associated malignant catarrhal fever (MCF), and to investigate a possible correlation between the severity and progression of certain typical clinical signs and disease outcome. PROCEDURE This prospective study involved cows diagnosed with MCF between 2007 and 2010, and included cows enrolled in a larger clinical trial. Signalment of affected cows, presence and progression of ocular lesions, as well as disease outcome were recorded. Cows were divided into two groups based on the disease outcome: (1) survival and (2) nonsurvival. The degree of corneal edema at first examination was statistically compared between groups. The progression of corneal edema, uveitis and the examining ophthalmologists' subjective assessment of disease progression between the first and last examination were also compared between groups. RESULTS Twenty-five cows (22 F, 3 M) of six different breeds and one mixed breed were included. Median age was 21 months (range 8-113). Ten cows survived, 13 cows were euthanized, and two died. No statistical association was identified between the degree of corneal edema at the first examination and the disease outcome. Deterioration of corneal edema was not noticed in any of the surviving animals. Anterior uveitis improved in all 10 surviving cows. CONCLUSIONS The degree of corneal edema at first examination had no prognostic value for the disease outcome. The results of this study suggest that the progression of corneal edema correlates well with disease outcome, and that nonimprovement of uveitis is a bad prognostic sign.
Gender specificity of geographical variation in morbid risk for schizophrenia in rural Ireland

The morbid risk for schizophrenia was determined separately for men and women within each of 36 contiguous district electoral divisions of a rural Irish county in which all cases of schizophrenia satisfying DSM‐III‐R criteria had been sought. The distribution of morbid risk values for men (0.0‐19.2 per 1000) was in accordance with a statistical model for random occurrences in space, while for women (0.0‐38.3 per 1000) it deviated markedly from such a model; thus, morbid risk for schizophrenia showed very prominent geographical variations in women, but not in men. These findings may have a basis in factors that can show both spatial inhomogeneity and gender specificity in their consequences for schizophrenia.