Miracle-dz/UniFAR-FTUs huggingface dataset:
Igf1 promotes longitudinal bone growth by insulin‐like actions augmenting chondrocyte hypertrophy

Longitudinal bone growth, and hence stature, are functions of growth plate chondrocyte proliferation and hypertrophy. Insulin‐like growth factor 1 (Igf1) is reputed to augment longitudinal bone growth by stimulating growth plate chondrocyte proliferation. In this study, however, we demonstrate that chondrocyte numbers and proliferation are normal in Igf1 null mice despite a 35% reduction in the rate of long bone growth. Igf1 null hypertrophic chondrocytes differentiate normally in terms of expressing specialized proteins such as collagen X and alkaline phosphatase, but are smaller than wild‐type at all levels of the hypertrophic zone. The terminal hypertrophic chondrocytes, which form the scaffold on which long bone growth extends, are reduced in linear dimension by 30% in Igf1 null mice, accounting for most of their decreased longitudinal growth. The expression of the insulin‐sensitive glucose transporter, GLUT4, is significantly decreased and the insulin‐regulated enzyme glycogen synthase kinase 3β (GSK3) is hypo‐phosphorylated in Igf1 null chondrocytes. Glycogen levels were significantly decreased and ribosomal RNA levels were reduced by almost 75% in Igf1 null chondrocytes. These data suggest that Igf1 promotes longitudinal bone growth by ‘insulin‐like’ anabolic actions which augment chondrocyte hypertrophy. — Wang, J., Zhou, J., Bondy, C. A. Igf1 promotes longitudinal bone growth by insulin‐like actions augmenting chondrocyte hypertrophy. FASEB J. 13, 1985–1990 (1999)
Leadership Behavior and Organizational Climate : An Empirical Study in a Non-profit Organization

The primary purpose of this research paper is to present an empirical study framed by the theory that task-oriented and relations-oriented leadership behaviors are positively related to the employees’ perceptions of organizational climate. The study examined the following research question: Are task-oriented and relations-oriented leadership behaviors related to different dimensions of organizational climate in a non-profit organization? The study introduces the theoretical perspective and examines the relevant literature that supports the significance of leadership behavior and organizational climate. The methodology for collecting the data was through the combination of two quantitative instruments into a web-based questionnaire consisting of 79 questions aimed at determining the relative contribution that the independent variables (task-oriented and relations-oriented leadership behaviors) have on the dependent variables (the different dimensions of organizational climate). The following control variables were collected from the sample and were statistically controlled in the data analysis: age, educational level, gender, job rank, and job tenure. The results of the study show that certain leadership behaviors do have an impact on a few dimensions of organizational climate.
The Murine Ortholog of Notchless, a Direct Regulator of the Notch Pathway in Drosophila melanogaster, Is Essential for Survival of Inner Cell Mass Cells

ABSTRACT Notch signaling is an evolutionarily conserved pathway involved in intercellular communication and is essential for proper cell fate choices. Numerous genes participate in the modulation of the Notch signaling pathway activity. Among them, Notchless (Nle) is a direct regulator of the Notch activity identified in Drosophila melanogaster. Here, we characterized the murine ortholog of Nle and demonstrated that it has conserved the ability to modulate Notch signaling. We also generated mice deficient for mouse Nle (mNle) and showed that its disruption resulted in embryonic lethality shortly after implantation. In late mNle−/− blastocysts, inner cell mass (ICM) cells died through a caspase 3-dependent apoptotic process. Most deficient embryos exhibited a delay in the temporal down-regulation of Oct4 expression in the trophectoderm (TE). However, mNle-deficient TE was able to induce decidual swelling in vivo and properly differentiated in vitro. Hence, our results indicate that mNle is mainly required in ICM cells, being instrumental for their survival, and raise the possibility that the death of mNle-deficient embryos might result from abnormal Notch signaling during the first steps of development.
Physician practices and readiness for medical home reforms: policy, pitfalls, and possibilities.

OBJECTIVE To determine the proportion of physician practices in the United States that currently meets medical home criteria. DATA SOURCE/STUDY SETTING 2007 and 2008 National Ambulatory Medical Care Survey. STUDY DESIGN We mapped survey items to the National Committee on Quality Assurance's (NCQA's) medical home standards. After awarding points for each "passed" element, we calculated a practice's infrastructure score, dividing its cumulative total by the number of available points. We identified practices that would be recognized as a medical home (Level 1 [25-49 percent], Level 2 [50-74 percent], or Level 3 [infrastructure score ≥75 percent]) and examined characteristics associated with NCQA recognition. RESULTS Forty-six percent (95 percent confidence interval [CI], 42.5-50.2) of all practices lack sufficient medical home infrastructure. While 72.3 percent (95 percent CI, 64.0-80.7 percent) of multi-specialty groups would achieve recognition, only 49.8 percent (95 percent CI, 45.2-54.5 percent) of solo/partnership practices meet NCQA standards. Although better prepared than specialists, 40 percent of primary care practices would not qualify as a medical home under present criteria. CONCLUSION Almost half of all practices fail to meet NCQA standards for medical home recognition.
Tropheryma whipplei endocarditis in Spain

Abstract Tropheryma whipplei endocarditis is an uncommon condition with very few series and <90 cases reported in the literature. The aim of the study was to analyze the epidemiological, clinical, and outcome characteristics of 17 cases of T. whipplei endocarditis recruited in our country from a multicentric cohort from 25 Spanish hospitals from the Spanish Collaboration on Endocarditis—Grupo de Apoyo al Manejo de la Endocarditis infecciosa en España. From a total of 3165 cases included in the cohort, 14.2% were diagnosed of blood culture negative endocarditis (BCNE) and 3.5% of these had T. whipplei endocarditis. This condition was more frequent in men. The average age was 60.3 years. Previous cardiac condition was present in 35.3% of the cases. The main clinical manifestation was cardiac failure (76.5%) while fever was only present in the 35.3%. Ecocardiography showed vegetations in 64.7% of patients. Surgery was performed in all but 1 cases and it allowed the diagnosis when molecular assays were performed. A broad range rRNA 16S polymerase chain reaction was used for first instance in all laboratories and different specific targets for T. whipplei were employed for confirmation. A concomitant Whipple disease was diagnosed in 11.9% of patients. All patients received specific antimicrobial treatment for at least 1 year, with no relapse and complete recovery. T. whipplei endocarditis is an uncommon condition with an atypical presentation that must be considered in the diagnosis of BCNE. The prognosis is very good when an appropriate surgical management and antimicrobial-specific treatment is given.
Pulse shape optimization in dispersion-limited direct detection optical fiber links

We study the theoretical limits of optical communication channels affected by chromatic dispersion. By using as a metric the energy transfer ratio, we find the optimal transmitted pulse shape that allows the impact of dispersion to be minimized. We show that these optimized pulses perform significantly better than standard nonreturn-to-zero/on-off keying (NRZ-OOK) modulation.
Fatigue tests on notched and unnotched sheet specimens of 2024-T3 and 7075-T6 aluminum alloys and of SAE 4130 steel with special consideration of the life range from 2 to 10,000 cycles

Note presenting fatigue tests performed on notched and unnotched sheet specimens made of 2024-T3 and 7075-T6 aluminum alloys and of SAE 4130 steel. The steel was tested in two conditions: normalized and heat-treated to a tensile strength of 180 ksi. The primary focus was on the life range from 2 to 10,000 cycles.
Renal replacement therapy in Europe: a summary of the 2013 ERA-EDTA Registry Annual Report with a focus on diabetes mellitus

Background This article provides a summary of the 2013 European Renal Association–European Dialysis and Transplant Association (ERA-EDTA) Registry Annual Report (available at http://www.era-edta-reg.org), with a focus on patients with diabetes mellitus (DM) as the cause of end-stage renal disease (ESRD). Methods In 2015, the ERA-EDTA Registry received data on renal replacement therapy (RRT) for ESRD from 49 national or regional renal registries in 34 countries in Europe and bordering the Mediterranean Sea. Individual patient data were provided by 31 registries, while 18 registries provided aggregated data. The total population covered by the participating registries comprised 650 million people. Results In total, 72 933 patients started RRT for ESRD within the countries and regions reporting to the ERA-EDTA Registry, resulting in an overall incidence of 112 per million population (pmp). The overall prevalence on 31 December 2013 was 738 pmp (n = 478 990). Patients with DM as the cause of ESRD comprised 24% of the incident RRT patients (26 pmp) and 17% of the prevalent RRT patients (122 pmp). When compared with the USA, the incidence of patients starting RRT pmp secondary to DM in Europe was five times lower and the incidence of RRT due to other causes of ESRD was two times lower. Overall, 19 426 kidney transplants were performed (30 pmp). The 5-year adjusted survival for all RRT patients was 60.9% [95% confidence interval (CI) 60.5–61.3] and 50.6% (95% CI 49.9–51.2) for patients with DM as the cause of ESRD.
Predicting the Metabolic Potential of the Novel Iron Oxidising Bacterium "Ferrovum" sp. JA12 Using Comparative Genomics

Here we describe the potential uptake and assimilation pathways for the essential nutrients C, N, P, and S in the acidophilic iron oxidiser Ferrovum strain JA12, a member of a novel genus among the Betaproteobacteria. Comparative genomics proved to be a powerful approach to give first insights into the biochemical potential of this novel genus and to understand the reasons for the dominating abundance of Ferrovum spp. in a pilot plant to remediate acid mine drainage.
EFFECTS OF TWO COGNITIVE-BEHAVIORAL PHYSICAL ACTIVITY AND NUTRITION TREATMENTS ON PSYCHOSOCIAL PREDICTORS OF CHANGES IN FRUIT/VEGETABLE AND HIGH-FAT FOOD INTAKE, AND WEIGHT

Improved mood may increase the consumption of healthy foods and decrease the intake of unhealthy foods. Increased physical activity might improve mood and, thus, eating behaviors. Adults (M age = 45 years) with severe and morbid obesity (M body mass index = 41kg/m 2 ) were randomly assigned to 6 months of either cognitive-behavioral physical activity and nutrition-support methods alone (n = 92), or those methods plus mood regulation training (n = 92). There were significant improvements in physical activity, mood, self-regulation and self- efficacy for controlling eating, and weight that did not differ by group. Improvement in mood was associated with greater fruit/vegetable intake. Change in self-efficacy significantly mediated that relationship. Only two sessions of moderate physical activity/week were required to improve mood. Findings have implications for weight-loss intervention improvement.
Analysis of the Binding Moiety Mediating the Interaction between Monocarboxylate Transporters and Carbonic Anhydrase II*

Background: Carbonic anhydrase II (CAII) augments activity of monocarboxylate transporters (MCTs) by noncatalytic interaction. Results: CAII binds to an acidic cluster with an appropriate context in the MCT C terminus. Conclusion: Isoform-specific interaction between MCTs and CAII requires a specific binding moiety. Significance: CAII-mediated increase in lactate transport depends on the presence of a specific binding moiety in MCT. Proton-coupled monocarboxylate transporters (MCTs) mediate the exchange of high energy metabolites like lactate between different cells and tissues. We have reported previously that carbonic anhydrase II augments transport activity of MCT1 and MCT4 by a noncatalytic mechanism, while leaving transport activity of MCT2 unaltered. In the present study, we combined electrophysiological measurements in Xenopus oocytes and pulldown experiments to analyze the direct interaction between carbonic anhydrase II (CAII) and MCT1, MCT2, and MCT4, respectively. Transport activity of MCT2-WT, which lacks a putative CAII-binding site, is not augmented by CAII. However, introduction of a CAII-binding site into the C terminus of MCT2 resulted in CAII-mediated facilitation of MCT2 transport activity. Interestingly, introduction of three glutamic acid residues alone was not sufficient to establish a direct interaction between MCT2 and CAII, but the cluster had to be arranged in a fashion that allowed access to the binding moiety in CAII. We further demonstrate that functional interaction between MCT4 and CAII requires direct binding of the enzyme to the acidic cluster 431EEE in the C terminus of MCT4 in a similar fashion as previously shown for binding of CAII to the cluster 489EEE in the C terminus of MCT1. In CAII, binding to MCT1 and MCT4 is mediated by a histidine residue at position 64. Taken together, our results suggest that facilitation of MCT transport activity by CAII requires direct binding between histidine 64 in CAII and a cluster of glutamic acid residues in the C terminus of the transporter that has to be positioned in surroundings that allow access to CAII.
Combining High-Resolution Images With Low-Quality Videos

Recently a lot of research has been directed towards the question: What can be done with “brute-force vision” using huge amounts of data? Image retrieval methods have been shown to succeed on collections of images with sizes over a million. Various applications such as object recognition, 3D geometrical arrangment of images showing the same scene or inferring missing image regions can benefit from large image databases. Motivated by this research we propose an alternative use of image information stored in large pools like the internet. Given an input video, we can utilize corresponding still images stored at much better quality to improve the overall quality of the video. A hybrid superresolution scheme is applied to smoothly incorporate the high-frequency components. On those areas where hallucination of details fails, a standard MAP-estimation of the high-resolution image is performed. The performance is demonstrated on real data examples.
Factors associated with spousal physical violence in Albania: cross sectional study

Abstract Objective To describe the prevalence of intimate partner violence and associated factors among married women in Albania. Design Cross sectional study. Setting Tirana, the capital city of Albania. Participants A representative sample of 1039 married women aged 25-65 living in Tirana and recorded in the 2001 census. Methods Questionnaire on intimate partner violence and social and demographic characteristics of the women and their husbands. Main outcome measure Women's experience within the past year of being hit, slapped, kicked, or otherwise physically hurt by the husband. Results More than a third (37%, 384/1039) of women had experienced violence. Risk was greatest among women aged 25-34 (odds ratio 1.47, 95% CI 1.04 to 2.09), women with tertiary education (3.70, 2.04 to 6.67), women in white collar jobs (4.0, 1.59 to 10.0), women with least educated husbands (5.01, 2.91 to 8.64), and women married to men raised in rural areas (3.31, 2.29 to 4.80). Women were at higher risk if they were more educated than their husbands (4.76, 2.56 to 9.09). Conclusions In transitional Albania, the risk of spousal violence is high, and more empowered women are at greater risk.
Involvement of Wee1 in the Circadian Rhythm–Dependent Intestinal Damage Induced by Docetaxel

Docetaxel, a semisynthetic taxane, is effective for the treatment of some solid cancers; however, docetaxel-induced intestinal damage leads to poor prognosis in some patients. Although such adverse effects have been reported to depend on the dosing-time of docetaxel, the mechanisms involved remain unclear. Wee1 expression is controlled by the clock gene complex, clock/bmal1, and contributes to cell-cycle progression. The present study was undertaken to evaluate the potential role of Wee1 in the circadian rhythm–dependent profile of docetaxel. Male mice were maintained under a 12-hour light/dark cycle. Intestinal damage after repeated dosing of docetaxel (20 mg/kg) for 3 weeks was more severe at 14 hours after light on (HALO) than at 2 HALO. The intestinal protein expressions of Wee1, phosphorylated CDK1, and cleaved Caspase-3 were higher in the 14-HALO group than in the 2-HALO group, whereas that of survivin was lower in the 14-HALO group. Thus, it is speculated that elevated Wee1 expression inhibited CDK1 activity more by phosphorylation, which in turn caused the lower expression of survivin and consequently more activated Caspase-3 in the 14-HALO group. There were no significant differences in plasma docetaxel concentrations between the 2- and 14-HALO groups. Bindings of CLOCK and BMAL1 to the E-box regions at the wee1 gene promoter were not altered by docetaxel treatment at 2 and 14 HALO. These findings suggest that Wee1 is directly or indirectly involved in the mechanism of the circadian rhythm–dependent changes in docetaxel-induced intestinal damage. However, the mechanism for a circadian rhythm–dependent change in intestinal Wee1 expression by docetaxel remains to be determined.
A level-set approach for the metamorphosis of solid models

We present a new approach to 3D shape metamorphosis. We express the interpolation of two shapes as a process where one shape deforms to maximize its similarity with another shape. The process incrementally optimizes an objective function while deforming an implicit surface model. We represent the deformable surface as a level set (iso-surface) of a densely sampled scalar function of three dimensions. Such level-set models have been shown to mimic conventional parametric deformable surface models by encoding surface movements as changes in the grayscale values of a volume data set. Thus, a well-founded mathematical structure leads to a set of procedures that describes how voxel values can be manipulated to create deformations that are represented as a sequence of volumes. The result is a 3D morphing method that offers several advantages over previous methods, including minimal need for user input, no model parameterization, flexible topology, and subvoxel accuracy.
Antibiotic prophylaxis in permanent pacemaker implantation: a prospective randomised trial.

BACKGROUND--Pacemaker pocket infection is a potentially serious problem after permanent pacemaker implantation. Antibiotic prophylaxis is commonly prescribed to reduce the incidence of this complication, but current trial evidence of its efficacy is conflicting. A large prospective randomised trial was therefore performed of antibiotic prophylaxis in permanent pacemaker implantation. The intention was firstly to determine whether antibiotic prophylaxis is efficacious in these patients and secondly to identify which patients are at the highest risk of infection. METHODS--A prospective randomised open trial of flucloxacillin (clindamycin if the patient was allergic to penicillin) v no antibiotic was performed in a cohort of patients undergoing first implantation of a permanent pacing system over a 17 month period. Intravenous antibiotics were started at the time of implantation and continued for 48 hours. The trial endpoint was a repeat operation for an infective complication. RESULTS--473 patients were entered into a randomised trial. 224 received antibiotic prophylaxis and 249 received no antibiotics. A further 183 patients were not randomised but were treated according to the operator's preference (64 antibiotics, 119 no antibiotics); these patients are included only in the analysis of predictors of infection. Patients were followed up for a mean (SD) of 19(5) months. Among the patients in the randomised group there were nine infections requiring a repeat operation, all in the group not receiving antibiotic (P = 0.003). In the total patient cohort there were 13 infections, all but one in the non-antibiotic group (P = 0.006). Nine of the infections presented as erosion of the pulse generator or electrode, three as septicaemia secondary to Staphylococcus aureus, and one as a pocket abscess secondary to Staphylococcus epidermidis. Infections were significantly more common when the operator was inexperienced (< or = 100 previous patients), the operation was prolonged, or after a repeat operation for non-infective complications (principally lead displacement). Infection was not significantly more common in patients identified preoperatively as being at high risk (for example patients with diabetes mellitus, patients receiving long term steroid treatment), although there was a trend in this direction. CONCLUSIONS--Antibiotic prophylaxis significantly reduced the incidence of infective complications requiring a repeat operation after permanent pacemaker implantation. It is suggested that antibiotics should be used routinely.
Anti-Aging Effects of Some Selected Iranian Folk Medicinal Herbs-Biochemical Evidences

Objective(s): In the current study, the effects of selected folk medicinal herbs were evaluated in D-galactose-induced aging in male mice. Materials and Methods: Male BALB/c mice were randomly divided into 12 groups composing sham, control, and treated groups. Aging was induced by administration of D-galactose (500 mg/kg/day for 6 weeks). A positive control group was assigned that received vitamin E (200 mg/kg/day). The extract of herbs was prepared, lyophilized, and used in this study. The herbs were administered by gavage for 4 weeks to D-galactose-aged animals at the selected doses (mg/kg/day) as follows: Zingiber officinale (250), Glycyrrhiza glabra (150), Rosmarinus officinalis (300), Peganum harmala (50), Aloe vera (150), Satureja hortensis (200), Teucrium scordium (200), Hypericum perforatum (135) and Silybum marianum (150). One group of animals was assigned as sham and not given D-galactose. Results: At the end of treatment, pro-inflammatory markers including tumor necrosis factor-α (TNF-α), interlukine-1β (IL-β), interlukine-6 (IL-6), NF-kappaB (NF-κb), total antioxidant power (TAP), thiobarbituric acid reactive substances (TBARS) as lipid peroxidation (LPO) marker and male sex hormones i.e. testosterone and dehydroepiandrosterone-sulfate (DHEA-S) were measured in the blood. Conclusion: These data for the first time indicate significant anti-aging potential of examined herbs. Results showed that D-galactose induces a significant oxidative stress and promotes proinflammatory cascade of aging while all herbs more or less recovered these changes. Among 9 herbal extracts, Silybum marianum showed the best effect in restoring aging changes.