Miracle-dz/UniFAR-FTUs huggingface dataset:
From Liking to Loyalty: The Impact of Network Affinity in the Social Media Digital Space

Building on existing models and social influence theory, we propose a hybrid social network affinity model which focuses on developing lasting relationships between consumers and social networking firms. Our model incorporates both rational and social influence motivations as antecedents to satisfaction and loyalty. Based on 352 adult respondents, we utilize a partial least squares structural equation method, and find that social network affinity is an important predictor of satisfaction and loyalty within a social network. In addition to other implications, our study indicates that social networking firms must build a sense of belonging, encourage interactivity, and offer ways to develop an emotional connection for their consumers.
Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts

Decitabine (DAC), an inhibitor of DNA methyltransferase, demonstrates antitumor activities in various types of cancer. However, its therapeutic potential for cholangiocarcinoma (CCA), one of the most aggressive gastrointestinal malignancies, remains to be explored. The present study investigated the antiproliferative effects of DAC on CCA cells in vitro and in vivo. Human CCA cell lines, TFK-1 and QBC939, were used as models to investigate DAC on the cell growth and proliferation of CCA. Cell proliferation was evaluated by Cell Counting Kit-8 assay combined with clonogenic survival assay. Flow cytometry, Hoechst 33342/propidium iodide staining and green fluorescent protein-tagged MAP-LC3 detection were applied to determine cell cycle progression, apoptosis and autophagy. Nude mice with TFK-1 xenografts were evaluated for tumor growth following DAC treatment. DAC was observed to significantly suppress the proliferation of cultured TFK-1 and QBC939 cells, accompanied with enhanced apoptosis, autophagy and cell cycle arrest at G2/M phase. In TFK-1 mouse xenografts, DAC retarded the tumor growth and increased the survival of CCA tumor-bearing mice.
Wave Pipelined Circuits Synthesis

Wave pipelining is a technique which can be used to speed up the circuit without insertion of storage elements, but because of that fact, needs to be more tightly controlled when being designed. This paper - taking the wave pipeline design constraints into account - looks to automate the generation of wave pipelined design netlists through synthesis and delay balancing scripts. The results show less than 20% delay deviation between maximum and minimum delays which was the target criteria and is comparable to numbers from hand-crafted designs
The Prion Protein Ligand, Stress-Inducible Phosphoprotein 1, Regulates Amyloid-β Oligomer Toxicity

In Alzheimer's disease (AD), soluble amyloid-β oligomers (AβOs) trigger neurotoxic signaling, at least partially, via the cellular prion protein (PrPC). However, it is unknown whether other ligands of PrPC can regulate this potentially toxic interaction. Stress-inducible phosphoprotein 1 (STI1), an Hsp90 cochaperone secreted by astrocytes, binds to PrPC in the vicinity of the AβO binding site to protect neurons against toxic stimuli. Here, we investigated a potential role of STI1 in AβO toxicity. We confirmed the specific binding of AβOs and STI1 to the PrP and showed that STI1 efficiently inhibited AβO binding to PrP in vitro (IC50 of ∼70 nm) and also decreased AβO binding to cultured mouse primary hippocampal neurons. Treatment with STI1 prevented AβO-induced synaptic loss and neuronal death in mouse cultured neurons and long-term potentiation inhibition in mouse hippocampal slices. Interestingly, STI1-haploinsufficient neurons were more sensitive to AβO-induced cell death and could be rescued by treatment with recombinant STI1. Noteworthy, both AβO binding to PrPC and PrPC-dependent AβO toxicity were inhibited by TPR2A, the PrPC-interacting domain of STI1. Additionally, PrPC–STI1 engagement activated α7 nicotinic acetylcholine receptors, which participated in neuroprotection against AβO-induced toxicity. We found an age-dependent upregulation of cortical STI1 in the APPswe/PS1dE9 mouse model of AD and in the brains of AD-affected individuals, suggesting a compensatory response. Our findings reveal a previously unrecognized role of the PrPC ligand STI1 in protecting neurons in AD and suggest a novel pathway that may help to offset AβO-induced toxicity.
Differential Recognition of P. falciparum VAR2CSA Domains by Naturally Acquired Antibodies in Pregnant Women from a Malaria Endemic Area

Background Plasmodium falciparum infected red blood cells (iRBC) express variant surface antigens (VSA) of which VAR2CSA is involved in placental sequestration and causes pregnancy-associated malaria (PAM). Primigravidae are most susceptible to PAM whereas antibodies associated with protection are often present at higher levels in multigravid women. However, HIV co-infection with malaria has been shown to alter this parity-dependent acquisition of immunity, with more severe symptoms as well as more malaria episodes in HIV positive women versus HIV negative women of a similar parity. Methods Using VAR2CSA DBL-domains expressed on the surface of CHO-745 cells we quantified levels of DBL-domain specific IgG in sera from pregnant Malawian women by flow cytometry. Dissociations constants of DBL5ε specific antibodies were determined using a surface plasmon resonance technique, as an indication of antibody affinities. Results VAR2CSA DBL5ε was recognized in a gender and parity-dependent manner with anti-DBL5ε IgG correlating significantly with IgG levels to VSA-PAM on the iRBC surface. HIV positive women had lower levels of anti-DBL5ε IgG than HIV negative women of similar parity. In primigravidae, antibodies in HIV positive women also showed significantly lower affinity to VAR2CSA DBL5ε. Conclusions Pregnant women from a malaria-endemic area had increased levels of anti-DBL5ε IgG by parity, indicating this domain of VAR2CSA to be a promising vaccine candidate against PAM. However, it is important to consider co-infection with HIV, as this seems to change the properties of antibody response against malaria. Understanding the characteristics of antibody response against VAR2CSA is undoubtedly imperative in order to design a functional and efficient vaccine against PAM.
Advertising on the Internet

Analizira se korpus reklamnih tekstova s hrvatskih internetskih stranica. Reklame se grupiraju tematski s obzirom na virtualni prostor, identitet i prikazivanje tjelesnosti. Naglasene su opce osobine reklamnoga diskursa te su izdvojene pojedine specificnosti reklamiranja na Internetu.
Proceedings of the Prague Stringology Conference 2009

A synchronizing word of a deterministic automaton is a word in the alphabet of colors (considered as letters) of its edges that maps the automaton to a single state. A coloring of edges of a directed graph is synchronizing if the coloring turns the graph into a deterministic finite automaton possessing a synchronizing word. The road coloring problem is the problem of synchronizing coloring of a directed finite strongly connected graph with constant outdegree of all its vertices if the greatest common divisor of lengths of all its cycles is one. The problem was posed by Adler, Goodwyn and Weiss over 30 years ago and evoked noticeable interest among the specialists in the theory of graphs, deterministic automata and symbolic dynamics. The positive solution of the road coloring problem is presented. Some consequences on the length of the synchronizing word are discussed.
Lactate promotes plasticity gene expression by potentiating NMDA signaling in neurons

Significance The transfer of lactate, a product of aerobic glycolysis, from astrocytes to neurons was recently shown to be necessary for the establishment of long-term memory and for the maintenance of in vivo long-term potentiation. Here, we report that lactate induces the expression of plasticity genes such as Arc, c-Fos, and Zif268 in neurons. The action of lactate is mediated by the modulation of NMDA receptor activity and the downstream Erk1/2 signaling cascade, through a mechanism associated with changes in the cellular redox state. These observations unveil an unexpected role of lactate as a signaling molecule in addition to its role in energy metabolism and open a previously unidentified research avenue for the study of neuronal plasticity and memory. l-lactate is a product of aerobic glycolysis that can be used by neurons as an energy substrate. Here we report that in neurons l-lactate stimulates the expression of synaptic plasticity-related genes such as Arc, c-Fos, and Zif268 through a mechanism involving NMDA receptor activity and its downstream signaling cascade Erk1/2. l-lactate potentiates NMDA receptor-mediated currents and the ensuing increase in intracellular calcium. In parallel to this, l-lactate increases intracellular levels of NADH, thereby modulating the redox state of neurons. NADH mimics all of the effects of l-lactate on NMDA signaling, pointing to NADH increase as a primary mediator of l-lactate effects. The induction of plasticity genes is observed both in mouse primary neurons in culture and in vivo in the mouse sensory-motor cortex. These results provide insights for the understanding of the molecular mechanisms underlying the critical role of astrocyte-derived l-lactate in long-term memory and long-term potentiation in vivo. This set of data reveals a previously unidentified action of l-lactate as a signaling molecule for neuronal plasticity.
Role of PGC-1α signaling in skeletal muscle health and disease

This paper reviews the current understanding of the molecular basis of the peroxisome proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α)–mediated pathway and discusses the role of PGC‐1α in skeletal muscle atrophy caused by immobilization. PGC‐1α is the master transcription regulator that stimulates mitochondrial biogenesis, by upregulating nuclear respiratory factors (NRF‐1, 2) and mitochondrial transcription factor A (Tfam), which leads to increased mitochondrial DNA replication and gene transcription. PGC‐1α also regulates cellular oxidant–antioxidant homeostasis by stimulating the gene expression of superoxide dismutase‐2 (SOD2), catalase, glutathione peroxidase 1 (GPx1), and uncoupling protein (UCP). Recent reports from muscle‐specific PGC‐1α overexpression underline the importance of PGC‐1α in atrophied skeletal muscle, demonstrate enhancement of the PGC‐1α mitochondrial biogenic pathway, and reduced oxidative damage. Thus, PGC‐1α appears to play a protective role against atrophy‐linked skeletal muscle deterioration.
Negative moments of characteristic polynomials of random GOE matrices and singularity-dominated strong fluctuations

We calculate the negative integer moments of the (regularized) characteristic polynomials of N × N random matrices taken from the Gaussian orthogonal ensemble (GOE) in the limit as N → ∞. The results agree nontrivially with a recent conjecture of Berry and Keating motivated by techniques developed in the theory of singularity-dominated strong fluctuations. This is the first example where nontrivial predictions obtained using these techniques have been proved.
Measuring urban attitudes embedded in microblogging data: shrinking versus growing cities

This paper explores the use of powerful new software tools and social media data that can be used to study the attitudes of people in urban places. In particular, it uses propensity scoring to develop matched pairs of mid-sized US cities in the North East and Midwest, where the most significant difference between each pair is that of population decline. This resulted in a group of fifty declining cities matched with fifty growing or stable cities. Over 300,000 Twitter posts were collected over the course of two months, each analysed for either positive or negative sentiment. After running difference-of-means tests, we found that sentiment in the declining cities does not differ in a statistically significant manner from that in stable and growing cities. These findings suggest that real opportunities exist the better to understand urban attitudes through sentiment analysis of Twitter data.
Attention and Conscious Perception

Are we conscious of more than what's in the “spotlight” of attention, or is consciousness limited to the content of attention? Recently several authors (DeBrigard & Prinz 2011; Prinz 2010; Dennett & Cohen 2012) have defended the view that attention to some object is necessary for conscious perception of that object. For each of these authors, attention acts like more than just a “spotlight on a stage. ” But none of them provides a robust account of this new way of attending. My project offers a new theory of diffuse attention that explains the apparent richness of experience. Accepting that there is a diffuse way of attending requires us to abandon the notion of attention as a spotlight. On the view that I offer, attention has degrees. For example, when looking at a landscape, your attention is spread over a broad spatial area and details are more difficult to remember or describe than when you focus attention in greater depth on some object within that landscape. A broad and shallow diffusion of attention nonetheless makes its object available for guiding thought and action, and so should be considered a way of attending rather than merely being conscious. After defending a theory of diffuse attention, I offer a new argument for the view that attention is necessary for conscious perception. My argument is motivated by the phenomenological observation iii. that ordinary perceptual experience has a structure: some objects are in the foreground of experience, while others are in the background. I motivate the claim that this foreground/background structure is necessary for perceptual experience, and argue that focal and diffuse attention provide the foreground/background structure. I conclude that attention is necessary for perceptual experience, since it provides a necessary structure of experience. In making this argument, I draw on phenomenological insight into the structure of consciousness from James (1890), Gurwitsch (1964; 1966) and C.O. Evans (1970). For each of these authors, attention structures the foreground – but not the background – of consciousness. My novel contribution is to provide an account of how attention structures the conscious background. By enriching the concept of attention to include diffuse attention, my account is poised to explain the structure of conscious experience from foreground to background.
Increased MDR1 Expression in Normal and Malignant Peripheral Blood Mononuclear Cells Obtained from Patients Receiving Depsipeptide (FR901228, FK228, NSC630176)

The increased expression of markers associated with a differentiated phenotype, such as P-glycoprotein (Pgp), follows treatment with histone deacetylase inhibitors. Because depsipeptide (FR901228, FK228, NSC630176) is a substrate for Pgp, up-regulation of the gene that encodes it, MDR1, would mean that depsipeptide induces its own mechanism of resistance. To examine the effect of depsipeptide on expression of ATP-binding cassette transporters associated with multidrug resistance, the kidney cancer cell lines 108, 121, 127, and 143 were treated with depsipeptide and evaluated by quantitative reverse transcription-PCR. Increased levels of MDR1 (1.3- to 6.3-fold) and ABCG2 (3.2- to 11.1-fold) but not MRP1 (0.9- to 1.3-fold) were observed. The induced Pgp transported the fluorescent substrates rhodamine 123, bisantrene, calcein-AM, BODIPY-vinblastine, and BODIPY-paclitaxel. In normal peripheral blood mononuclear cells (PBMC) and circulating tumor cells obtained from patients receiving depsipeptide, increased levels of histone H3 acetylation were found. We next examined MDR1 levels in normal and malignant PBMCs obtained from 15 patients enrolled in clinical trials with depsipeptide and detected up to a 6-fold increase in normal PBMCs and up to an 8-fold increase in circulating tumor cells after depsipeptide administration. In one patient with Sézary syndrome, increased MDR1 gene expression was accompanied by increased cell surface Pgp expression in circulating Sézary cells as determined by measurement of MRK-16 staining by flow cytometry. These studies suggest that depsipeptide induces its own mechanism of resistance and thus provide a basis for clinical trials evaluating depsipeptide in combination with a Pgp inhibitor.
PRP Augmentation for ACL Reconstruction

Current research is investigating new methods to enhance tissue healing to speed up recovery time and decrease the risk of failure in Anterior Cruciate Ligament (ACL) reconstructive surgery. Biological augmentation is one of the most exploited strategies, in particular the application of Platelet Rich Plasma (PRP). Aim of the present paper is to systematically review all the preclinical and clinical papers dealing with the application of PRP as a biological enhancer during ACL reconstructive surgery. Thirty-two studies were included in the present review. The analysis of the preclinical evidence revealed that PRP was able to improve the healing potential of the tendinous graft both in terms of histological and biomechanical performance. Looking at the available clinical evidence, results were not univocal. PRP administration proved to be a safe procedure and there were some evidences that it could favor the donor site healing in case of ACL reconstruction with patellar tendon graft and positively contribute to graft maturation over time, whereas the majority of the papers did not show beneficial effects in terms of bony tunnels/graft area integration. Furthermore, PRP augmentation did not provide superior functional results at short term evaluation.
Reduced Theta Connectivity during Set-Shifting in Children with Autism

Autism spectrum disorder (ASD) is a characterized by deficits in social cognition and executive function. An area of particular difficulty for children with ASD is cognitive flexibility, such as the ability to shift between attentional or response sets. The biological basis of such deficits remains poorly understood, although atypical development of structural and functional brain connectivity have been reported in ASD, suggesting that disruptions of normal patterns of inter-regional communication may contribute to cognitive problems in this group. The present magnetoencephalography study measured inter-regional phase synchronization while children with ASD and typically developing matched controls (6–14 years of age) performed a set-shifting task. Reduced theta-band phase synchronization was observed in children with ASD during extradimensional set-shifting. This reduction in task-dependent inter-regional connectivity encompassed numerous areas including multiple frontal lobe regions, and indicates that problems with communication among brain areas may contribute to difficulties with executive function in ASD.
Vascular calcification and cardiovascular function in chronic kidney disease.

BACKGROUND Vascular calcification and arterial stiffening are independent predictors of all causes and cardiovascular mortality in chronic kidney disease (CKD). Few data are currently available comparing vascular calcification and its attendant functional cardiovascular consequences between CKD stage 4 patients and both peritoneal dialysis (PD) and haemodialysis (HD) (CKD stage 5) patients. METHOD We studied 134 subjects (60 HD, 28 PD and 46 CKD 4). Vascular calcification was quantified using multi-slice spiral CT scanning of a 5 cm standardized segment of superficial femoral artery. Pulse wave analysis and pulse wave velocity were assessed using applanation tonometry, to determine arterial compliance. Further digital arterial pulse wave analysis was used to measure systemic haemodynamic variables. All medications were recorded and biochemical variables were time averaged for the 6 months prior to entering the study. RESULTS Forty-seven percent of CKD 4 patients demonstrated vascular calcification as compared with CKD 5 (71% PD and 73% HD, P = 0.02). HD patients had higher calcification scores (median 121) than either PD (median 21) or CKD 4 (median 0) (P = 0.008). There were no significant differences in baseline characteristics between the groups. Comparing tertiles of patients (based on calcification score), increased calcification score was associated with a reduction in arterial compliance (mean PWV 8.9 +/- 1.1, 11 +/- 3.6, 11.3 +/- 3.7 m/s, P = 0.005). The degree of calcification did not influence systolic blood pressure (BP), diastolic BP or heart rate. However, more heavily calcified patients demonstrated significantly higher mean pulse pressures (58 +/- 19, 74 +/- 22 and 72 +/- 25 mmHg, P = 0.001), lower total peripheral resistance (1.5 +/- 1, 1.3 +/- 0.8, 0.9 +/- 0.4, P = 0.01) and higher stroke volume (84 +/- 25, 95 +/- 29, 106 +/- 39 ml, P = 0.01). More heavily calcified patients were significantly older and predominantly male. CONCLUSION This study has successfully utilized a novel technique for the quantification of calcification. We have demonstrated vascular calcification and associated cardiovascular dysfunction in CKD 4, PD and HD with significant differences between the groups. Thirty percent of individuals show no calcification, even those established on renal replacement therapy for a prolonged period of time. Further work is required to identify factors which promote progression of arterial calcification in those who are susceptible.
Profound Effects of Population Density on Fitness-Related Traits in an Invasive Freshwater Snail

Population density can profoundly influence fitness-related traits and population dynamics, and density dependence plays a key role in many prominent ecological and evolutionary hypotheses. Here, we evaluated how individual-level changes in population density affect growth rate and embryo production early in reproductive maturity in two different asexual lineages of Potamopyrgus antipodarum, a New Zealand freshwater snail that is an important model system for ecotoxicology and the evolution of sexual reproduction as well as a potentially destructive worldwide invader. We showed that population density had a major influence on individual growth rate and early-maturity embryo production, effects that were often apparent even when comparing treatments that differed in population density by only one individual. While individual growth rate generally decreased as population density increased, we detected a hump-shaped relationship between embryo production and density, with females from intermediate-density treatments producing the most embryos and females from low- and high-density treatments producing the fewest embryos. The two lineages responded similarly to the treatments, indicating that these effects of population density might apply more broadly across P. antipodarum. These results indicate that there are profound and complex relationships between population density, growth rate, and early-maturity embryo production in at least two lineages of this important model system, with potential implications for the study of invasive populations, research on the maintenance of sex, and approaches used in ecotoxicology.