Miracle-dz/UniFAR-FTUs huggingface dataset:
Increasing the iodine concentration in the Swiss iodized salt program markedly improved iodine status in pregnant women and children: a 5-y prospective national study.

BACKGROUND Many industrialized countries struggle to maintain adequate iodine intake because of changes in dietary habits and the food supply. In Switzerland, because of declining iodine intakes in children and pregnant women, the iodine concentration in table salt was increased from 15 to 20 mg/kg. OBJECTIVE We evaluated Swiss iodine nutrition after the 1999 increase in the salt iodine concentration. DESIGN In 1999 and 2004, a 3-stage probability proportionate-to-size cluster sampling was done to obtain a representative national sample of primary schoolchildren and pregnant women. Urine and household salt were collected for iodine measurement. The frequency of elevated thyrotropin concentrations found in the newborn screening program was evaluated before and after the increase. RESULTS In 1999, median urinary iodine (UI) concentrations among children (n = 610) and pregnant women (n = 511) were 115 microg/L (range: 5-413 microg/L) and 138 microg/L (range: 5-1881 microg/L), respectively, which indicated marginal iodine status. In 2004, median UI concentrations among children (n = 386) and pregnant women (n = 279) were 141 microg/L (range: 0-516 microg/L) and 249 microg/L (range: 8-995 microg/L), respectively (P < 0.01). Newborn thyrotropin concentrations >5 mU/L decreased from 2.9% in 1992-1998 (n = 259 035) to 1.7% in 1999-2004 (n = 218 665) (P < 0.0001). CONCLUSIONS A 25% increase in iodine concentration in iodized table salt markedly improved iodine status in Switzerland, which showed the value of monitoring and adjusting iodine concentrations in national salt programs. The frequency of newborn thyrotropin concentrations >5 mU/L appears to be a sensitive indicator of iodine nutrition during pregnancy.
On Convergence of some Gradient-based Temporal-Differences Algorithms for Off-Policy Learning

We consider off-policy temporal-difference (TD) learning methods for policy evaluation in Markov decision processes with finite spaces and discounted reward criteria, and we present a collection of convergence results for several gradient-based TD algorithms with linear function approximation. The algorithms we analyze include: (i) two basic forms of two-time-scale gradient-based TD algorithms, which we call GTD and which minimize the mean squared projected Bellman error using stochastic gradient-descent; (ii) their "robustified" biased variants; (iii) their mirror-descent versions which combine the mirror-descent idea with TD learning; and (iv) a single-time-scale version of GTD that solves minimax problems formulated for approximate policy evaluation. We derive convergence results for three types of stepsizes: constant stepsize, slowly diminishing stepsize, as well as the standard type of diminishing stepsize with a square-summable condition. For the first two types of stepsizes, we apply the weak convergence method from stochastic approximation theory to characterize the asymptotic behavior of the algorithms, and for the standard type of stepsize, we analyze the algorithmic behavior with respect to a stronger mode of convergence, almost sure convergence. Our convergence results are for the aforementioned TD algorithms with three general ways of setting their $\lambda$-parameters: (i) state-dependent $\lambda$; (ii) a recently proposed scheme of using history-dependent $\lambda$ to keep the eligibility traces of the algorithms bounded while allowing for relatively large values of $\lambda$; and (iii) a composite scheme of setting the $\lambda$-parameters that combines the preceding two schemes and allows a broader class of generalized Bellman operators to be used for approximate policy evaluation with TD methods.
Nutrient Interactions and Toxicity Research Communication Excess Dietary Methionine Markedly Increases the Vitamin B-6 Requirement of Young Chicks

A soy-protein isolate diet that contained essentially no bioavailable vitamin B-6 was used to establish the quantitative effect of excess dietary methionine on the vitamin B-6 requirement of young chicks. When made adequate in vitamin B-6, chicks fed the basal diet required 2 g/kg supplemental DL-methionine to achieve maximal growth, and 10 g/kg additional DL-methionine (total 5 12 g/kg) was found to be a tolerable excess level that would not depress voluntary food intake or growth rate. When chicks were fed seven graded doses of supplemental pyridoxine (PN) in diets that contained either adequate (2 g/kg) or excess (12 g/kg) methionine, the vitamin B-6 requirement for maximal growth was found to increase (P < 0.01) from 0.73 to 1.05 mg/kg, a 44% increase, when 10 g/kg excess methionine was present in the diet. Indeed, this level of excess dietary methionine depressed (P < 0.01) growth at all PN dose levels ≤1 mg/kg, but not at PN doses of 1.2 or 1.4 mg/kg. Because dietary intakes of both vitamin B-6 and methionine can affect plasma homocysteine levels, dietary methionine (and protein) intake should be considered important factors in setting safe and adequate requirement levels for vitamin B-6. J. Nutr. 130: 3055–3058, 2000.
Testing concordance of instrumental variable effects in generalized linear models with application to Mendelian randomization

Instrumental variable regression is one way to overcome unmeasured confounding and estimate causal effect in observational studies. Built on structural mean models, there has been considerable work recently developed for consistent estimation of causal relative risk and causal odds ratio. Such models can sometimes suffer from identification issues for weak instruments. This hampered the applicability of Mendelian randomization analysis in genetic epidemiology. When there are multiple genetic variants available as instrumental variables, and causal effect is defined in a generalized linear model in the presence of unmeasured confounders, we propose to test concordance between instrumental variable effects on the intermediate exposure and instrumental variable effects on the disease outcome, as a means to test the causal effect. We show that a class of generalized least squares estimators provide valid and consistent tests of causality. For causal effect of a continuous exposure on a dichotomous outcome in logistic models, the proposed estimators are shown to be asymptotically conservative. When the disease outcome is rare, such estimators are consistent because of the log‐linear approximation of the logistic function. Optimality of such estimators relative to the well‐known two‐stage least squares estimator and the double‐logistic structural mean model is further discussed. Copyright © 2014 John Wiley & Sons, Ltd.
The Slx5-Slx8 Complex Affects Sumoylation of DNA Repair Proteins and Negatively Regulates Recombination

ABSTRACT Recombination is important for repairing DNA lesions, yet it can also lead to genomic rearrangements. This process must be regulated, and recently, sumoylation-mediated mechanisms were found to inhibit Rad51-dependent recombination. Here, we report that the absence of the Slx5-Slx8 complex, a newly identified player in the SUMO (small ubiquitin-like modifier) pathway, led to increased Rad51-dependent and Rad51-independent recombination. The increases were most striking during S phase, suggesting an accumulation of DNA lesions during replication. Consistent with this view, Slx8 protein localized to replication centers. In addition, like SUMO E2 mutants, slx8Δ mutants exhibited clonal lethality, which was due to the overamplification of 2μm, an extrachromosomal plasmid. Interestingly, in both SUMO E2 and slx8Δ mutants, clonal lethality was rescued by deleting genes required for Rad51-independent recombination but not those involved in Rad51-dependent events. These results suggest that sumoylation negatively regulates Rad51-independent recombination, and indeed, the Slx5-Slx8 complex affected the sumoylation of several enzymes involved in early steps of Rad51-independent recombination. We propose that, during replication, the Slx5-Slx8 complex helps prevent DNA lesions that are acted upon by recombination. In addition, the complex inhibits Rad51-independent recombination via modulating the sumoylation of DNA repair proteins.
Carbonated beverages and urinary calcium excretion.

BACKGROUND Intake of carbonated beverages has been associated with increased fracture risk in observational studies. The usual explanation given is that one or more of the beverage constituents increase urinary calcium. OBJECTIVE We assessed the short-term effects on urinary calcium excretion of carbonated beverages of various compositions. DESIGN An incomplete random block design was used to study 20-40-y-old women who customarily consumed > or =680 mL carbonated beverages daily. Four carbonated beverages were tested: 2 with caffeine and 2 without. Two contained phosphoric acid as the acidulant and 2 contained citric acid. The study included one neutral control (water) and one positive control (skim or chocolate milk). Serving size was 567 mL for the carbonated beverages and water and 340 mL for the milks. Beverages were consumed with a light breakfast after an overnight fast; no other foods were ingested until urine collection was complete. pH, titratable and total acidity, sodium, creatinine, and calcium were measured in 2-h (morning) fasting and 5-h postbeverage urine specimens. RESULTS Relative to water, urinary calcium rose significantly only with the milks and the 2 caffeine-containing beverages. The excess calciuria was approximately 0.25 mmol, about the same as previously reported for caffeine alone. Phosphoric acid without caffeine produced no excess calciuria; nor did it augment the calciuria of caffeine. CONCLUSIONS The excess calciuria associated with consumption of carbonated beverages is confined to caffeinated beverages. Acidulant type has no acute effect. Because the caffeine effect is known to be compensated for by reduced calciuria later in the day, we conclude that the net effect of carbonated beverage constituents on calcium economy is negligible. The skeletal effects of carbonated beverage consumption are likely due primarily to milk displacement.
Adipose tissue in muscle: a novel depot similar in size to visceral adipose tissue.

BACKGROUND The manner in which fat depot volumes and distributions, particularly the adipose tissue (AT) between the muscles, vary by race is unknown. OBJECTIVE The objective was to quantify a previously unstudied and novel intermuscular AT (IMAT) depot and subcutaneous AT, visceral AT (VAT), and total-body skeletal muscle mass in healthy sedentary African American (AA), Asian, and white adults by whole-body magnetic resonance imaging. IMAT is the AT between muscles and within the boundary of the muscle fascia. DESIGN Analyses were conducted on 227 women [AA (n = 79): body mass index (BMI; in kg/m(2)), 29.0 +/- 5.5; age, 45.7 +/- 16.9 y; Asian (n = 38): BMI, 21.7 +/- 2.9; age, 47.2 +/- 19.9 y; whites (n = 110): BMI, 24.9 +/- 5.4; age, 43.7 +/- 16.2 y]) and 111 men [AA (n = 39): BMI, 25.6 +/- 3.2; age, 45.5 +/- 18.8 y; Asian (n = 13): BMI, 24.9 +/- 2.5; age, 45.6 +/- 25.0 y; white (n = 59): BMI, 25.8 +/- 3.8; age 44.5 +/- 16.3 y]. RESULTS IMAT depots were not significantly different in size between race groups at low levels of adiposity; however, with increasing adiposity, AAs had a significantly greater increment in the proportion of total AT (TAT) than did the whites and Asians (58, 46, and 44 g IMAT/kg TAT, respectively; P = 0.001). VAT depots were not significantly different in size at low levels of adiposity but, with increasing adiposity, VAT accumulation was greater than IMAT accumulation in the Asians and whites; no significant differences were observed in AAs. CONCLUSION Race differences in AT distribution extend to IMAT, a depot that may influence race-ethnicity differences in dysglycemia.
Long-term moderate zinc supplementation increases exchangeable zinc pool masses in late-middle-aged men: the Zenith Study.

BACKGROUND Zinc supplementation may be beneficial for health. Assessing exchangeable zinc pools may be a useful approach to evaluate zinc status. OBJECTIVE We evaluated the effects of long-term supplementation with 2 moderate doses of zinc on the mass of exchangeable zinc pools. DESIGN Three groups of healthy, late-middle-aged men (n = 16 per group) participated in a stable-isotope zinc kinetic study after 6 mo of daily supplementation with 0 (placebo), 15, or 30 mg Zn. At the end of the supplementation period, each subject received an intravenous injection of 0.89 mg (70)Zn, and the plasma zinc disappearance curve was monitored for the next 10 d. Two approaches were used to determine the characteristics of the exchangeable zinc pools: 1) formal 3-compartmental modeling and 2) a simplified determination of the total mass of the rapidly exchangeable zinc pool (EZP). RESULTS In the placebo group, the exchangeable zinc pool masses for the 3 considered pools were as follows: 2.15, 12.7, and 100.5 mg Zn. The rapidly exchangeable zinc pool mass in the placebo group was 143 mg Zn. Zinc supplementation significantly increased the exchangeable zinc pool masses regardless of the approach used to determine these pools. In addition, these data confirm that exchangeable zinc pool masses correlate positively with total zinc intake and negatively with subject age and do not correlate with plasma zinc concentrations. CONCLUSION Our data show that long-term supplementation with 2 moderate doses of zinc is an efficient way to increase exchangeable zinc pool masses in late-middle-aged men.
Oral calcium suppresses biochemical markers of bone resorption in normal men.

Calcium supplementation decreases bone resorption and retards bone loss in women. There is little information about the effects of calcium supplementation in men. The effects of a 1-g oral calcium load at 0900 on bone-related biochemical variables were evaluated in 13 normal men (aged 51-70 y). Calcium administration was associated with increases in plasma ionized calcium (P < 0.001) and urinary calcium (P < 0.001), and a decrease in plasma parathyroid hormone (P < 0.001). There was a nonsignificant trend (r = -0.47, P = 0.11) for the decrease in plasma parathyroid hormone to be related to radiocalcium absorption. After the calcium load there were decreases in the urinary hydroxyproline-creatinine ratio from 11 +/- 1.1 to 7.9 +/- 0.6 (P < 0.01), the urinary deoxypyridinoline-creatinine ratio from 14.0 +/- 1.8 to 10.1 +/- 0.9 (P < 0.05), and the urinary pyridinoline-creatinine ratio from 52 +/- 5 to 40 +/- 3 (P < 0.01) between baseline and 6 h. There was no change in plasma osteocalcin. These observations indicate that a 1-g calcium load suppresses biochemical markers of bone resorption for > or = 6 h in normal men and support the concept that calcium supplementation may be useful in the prevention of bone loss in men.
Contract-based cooperative spectrum sharing

Providing proper economic incentives is essential for the success of dynamic spectrum sharing. Cooperative spectrum sharing is one effective way to achieve this goal. In cooperative spectrum sharing, secondary users (SUs) relay traffics for primary users (PUs), in exchange for dedicated transmission time for the SUs' own communication needs. In this paper, we study the cooperative spectrum sharing under incomplete information, where SUs' types (which capture the relay channel gains and the SUs' power costs) are private information and are not known to the PU. Inspired by the contract theory, we model the network as a labor market. The PU is an employer who offers a contract to the SUs. The contract consists of a set of items representing combinations of spectrum access time (i.e., reward) and relay power (i.e., contribution). The SUs are employees, and each of them selects the best contract item to maximize its payoff. We study the optimal contract design for both weakly and strongly incomplete information scenarios. First, we provide necessary and sufficient conditions for feasible contracts in both scenarios. In the weakly incomplete information scenario, we further derive the optimal contract that achieves the same maximum PU's utility as in the complete information benchmark. In the strongly incomplete information scenario, we propose a Decompose-and-Compare algorithm that achieves a close-to-optimal contract. We further show that the PU's expected utility loss due to the suboptimal algorithm and the strongly incomplete information are both relatively small (less than 2% and 1.3%, respectively, in our numerical results with two SU types).
Reliability and concurrent validity of standardized walking obstacle course test in people with stroke.

OBJECTIVES To investigate: (i) the intra-rater, inter-rater and test-retest reliabilities of completion times and step counts on the Standardized Walking Obstacle Course test (SWOC); (ii) correlations between SWOC scores and stroke-specific impairments; (iii) the cut-off SWOC completion times and step counts for distinguishing differences in obstacle negotiation ability in people with chronic stroke and healthy older adults. DESIGN Cross-sectional study. SETTING University-based rehabilitation centre. SUBJECTS Twenty-nine people with stroke and 30 healthy older adults. METHODS SWOC completion times and step counts were measured under 3 conditions: (i) normal walking; (ii) walking with a tray; and (iii) walking with dark-glasses. The Fugl-Meyer Assessment of lower extremity, the Five-Times-Sit-to-Stand Test, the Berg Balance Scale, the Timed Up and Go Test (TUG), and the Community Integration Measure questionnaire, were also administered. RESULTS SWOC completion times and step counts showed very satisfactory to excellent reliabilities (intraclass correlation coefficient; ICC = 0.851-0.993). TUG times correlated significantly with SWOC completion times and step counts under the 3 conditions (r = 0.586-0.815, p < 0.001). SWOC completion times of 14.73-16.00 s and step counts of 23.06-24.13, depending on different walking conditions, were able to discriminate between stroke survivors and healthy older adults. CONCLUSION The SWOC is a reliable clinical tool for assessing obstacle negotiation ability in people with stroke.
Bacteriophage Interactions with Marine Pathogenic Vibrios: Implications for Phage Therapy

A global distribution in marine, brackish, and freshwater ecosystems, in combination with high abundances and biomass, make vibrios key players in aquatic environments, as well as important pathogens for humans and marine animals. Incidents of Vibrio-associated diseases (vibriosis) in marine aquaculture are being increasingly reported on a global scale, due to the fast growth of the industry over the past few decades years. The administration of antibiotics has been the most commonly applied therapy used to control vibriosis outbreaks, giving rise to concerns about development and spreading of antibiotic-resistant bacteria in the environment. Hence, the idea of using lytic bacteriophages as therapeutic agents against bacterial diseases has been revived during the last years. Bacteriophage therapy constitutes a promising alternative not only for treatment, but also for prevention of vibriosis in aquaculture. However, several scientific and technological challenges still need further investigation before reliable, reproducible treatments with commercial potential are available for the aquaculture industry. The potential and the challenges of phage-based alternatives to antibiotic treatment of vibriosis are addressed in this review.
Building dependable COTS microkernel-based systems using MAFALDA

MAFALDA (Microkernel Assessment by Fault injection Analysis and Design Aid) is a generic tool providing quantitative information on COTS microkernels to support their integration into dependable systems. The main originality of MAFALDA relies on the features provided for both the analysis of the failure modes of the target microkernel and the design of error confinement wrappers. The paper illustrates: (i) how MAFALDA is organized and its user interface, and (ii) how it can be used to carry out fault injection campaigns. Finally, we present the experimental context of campaigns carried out on two commercial microkernels and draw the main lessons learnt.
Mast cell disorders in Ehlers–Danlos syndrome

Well known for their role in allergic disorders, mast cells (MCs) play a key role in homeostatic mechanisms and surveillance, recognizing and responding to different pathogens, and tissue injury, with an array of chemical mediators. After being recruited to connective tissues, resident MCs progenitors undergo further differentiation, under the influence of signals from surrounding microenvironment. It is the differential tissue homing and local maturation factors which result in a diverse population of resident MC phenotypes. An abundance of MC reside in connective tissue that borders with the external world (the skin as well as gastrointestinal, respiratory, and urogenital tracts). Situated near nerve fibers, lymphatics, and blood vessels, as well as coupled with their ability to secrete potent mediators, MCs can modulate the function of local and distant structures (e.g., other immune cell populations, fibroblasts, angiogenesis), and MC dysregulation has been implicated in immediate and delayed hypersensitivity syndromes, neuropathies, and connective tissue disorders (CTDs). This report reviews basic biology of mast cells and mast cell activation as well as recent research efforts, which implicate a role of MC dysregulation beyond atopic disorders and in a cluster of Ehlers–Danlos Syndromes, non‐IGE mediated hypersensitivity disorders, and dysautonomia. © 2017 Wiley Periodicals, Inc.
Ultra-thin and strong formvar-based membranes with controlled porosity for micro- and nano-scale systems

We present a methodology for developing ultra-thin and strong formvar-based membranes with controlled morphologies. Formvar is a thin hydrophilic and oleophilic polymer inert to most chemicals and resistant to radiation. The formvar-based membranes are viable materials as support structures in micro- and macro-scale systems depending on thinness and porosity control. Tunable sub-micron thick porous membranes with 20%–65% porosity were synthesized by controlling the ratios of formvar, glycerol, and chloroform. This synthesis process does not require complex separation or handling methods and allows for the production of strong, thin, and porous formvar-based membranes. An expansive array of these membrane characterizations including chemical compatibility, mechanical responses, wettability, as well as the mathematical simulations as a function of porosity has been presented. The wide range of chemical compatibility allows for membrane applications in various environments, where other polymers would not be suitable. Our formvar-based membranes were found to have an elastic modulus of 7.8 GPa, a surface free energy of 50 mN m−1 and an average thickness of 125 nm. Stochastic model simulations indicate that formvar with the porosity of ∼50% is the optimal membrane formulation, allowing the most material transfer across the membrane while also withstanding the highest simulated pressure loadings before tearing. Development of novel, resilient and versatile membranes with controlled porosity offers a wide range of exciting applications in the fields of nanoscience, microfluidics, and MEMS.