Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical significance of serum p53 antigen in patients with pancreatic carcinomas.

BACKGROUND: Alterations in the p53 gene are often found in pancreatic cancer, and accumulation of the p53 protein has been noted in tumour cells. AIMS: To investigate whether serum p53 protein concentrations could be used as markers for p53 gene mutations in neoplasms of the pancreas. METHODS: Serum p53 protein concentrations were determined by an enzyme linked immunosorbent assay (ELISA) in 104 cases of pancreatic adenocarcinoma, and 61 matched formalin fixed tissue sections were also stained by an anti-p53 DO-7 monoclonal antibody. RESULTS: The mean serum concentration of p53 protein in the adenocarcinoma patients was 0.27 (SEM 0.02) ng/ml, and was significantly higher than in 35 healthy blood donors (0.15 (0.02) ng/ml, SD = 0.11) or in 15 cases of chronic pancreatitis (0.15 (0.02) ng/ml). Adopting an arbitrary cut off value for the serum p53 protein concentration of 0.37 ng/ml, which corresponded to a value 2 SD above the mean value from the healthy blood donors, positive serum p53 protein concentrations were found in 23 out of 104 (22.1%) patients with adenocarcinomas examined, 16 out of 47 (34.0%) patients with carcinomas with distant metastases, but only seven of 57 patients (12.3%) with carcinomas without metastases (p < 0.05). In 11 patients with pancreatic adenocarcinomas, the mean serum p53 protein concentration after tumour resection was 0.21 (0.05) ng/ml, and had decreased compared with the preoperative concentrations (0.25 (0.05) ng/ml) (P < 0.05). There were no significant associations between the serum concentrations of p53 protein and serum concentrations of markers such as CA19-9 or CEA; however, serum concentrations of p53 protein demonstrated a potential role as an additional tumour marker. Immunohistochemical studies disclosed that the p53 protein was expressed in 28 out of 61 pancreatic adenocarcinomas (45.9%). Serum p53 protein concentrations in the positively immunostained cases were significantly higher than in the negatively immunostained cases (0.35 (0.05) ng/ml v 0.15 (0.01) ng/ml; p < 0.005). Furthermore, positive immunostaining for p53 protein was found in eight out of 10 (80%) serum positive p53 protein cases with adenocarcinomas. CONCLUSION: An increase in serum p53 protein concentrations appears during the progression of pancreatic adenocarcinoma and correlates with the accumulation of p53 protein as a result of a mutation of the p53 gene. An analysis of p53 antigen concentrations can detect p53 gene alterations, which could be useful for the selection of treatment regimens.
Oncotargets and Therapy Dovepress Dovepress Salinomycin Repressed the Epithelial–mesenchymal Transition of Epithelial Ovarian Cancer Cells via Downregulating Wnt/β-catenin Pathway

hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms Abstract: Epithelial ovarian cancer (EOC) is the leading cause of death among all gynecological malignancies. Most patients are diagnosed in the advanced stage and have distant metastasis ultimately. Salinomycin has been demonstrated to reduce invasive capacity of multiple tumor cells. The objective of this study was to investigate the effects of salinomycin on EOC cells. The cell counting kit 8 (CCK-8) and Boyden chamber assays showed that salinomycin could effectively reduce the abilities of proliferation, migration and invasion in EOC cells. The western blot assay showed that salinomycin could increase the expression of epithelial markers (E-cadherin and Keratin) while decrease the expression of mesenchymal markers (N-cadherin and vimentin) in a dose-dependent manner. These results were ascertained by reverse transcription polymerase chain reaction (RT-PCR). Besides, salinomycin could downregulate the expression of proteins associated with the Wnt/β-catenin pathway and repress the nuclear translocation of β-catenin. It was also shown that salinomycin could reverse the aberrant activation of the canonical Wnt pathway induced by GSK-3β inhibitor (SB216763). Our results revealed that salinomycin could inhibit the proliferation, migration and invasion in EOC cells. In addition, the inhibitive effect of salinomycin on the invasive ability was mediated by repressing the epithelial–mesenchymal transition (EMT) program, which may be achieved through its inhibition of the Wnt/β-catenin pathway.
The Distribution of Secondary Masses in Post-Common-Envelope Binaries: A Potential Test of Disrupted Magnetic Braking

The distribution of secondary star masses in present-day post-common-envelope binaries (PCEBs) is calculated using four different models for angular momentum loss (AML) during the post-common-envelope phase: only gravitational radiation (GR), GR plus disrupted magnetic braking (DMB), GR plus reduced MB, and GR plus intermediate MB. For the DMB model, we find that the number of PCEBs decreases abruptly by 38% once MB begins to operate for non-fully convective secondaries. We do not find a similar feature in the distributions calculated using any of the other three AML models in which MB is not disrupted. This percentage decrease in the number of present-day PCEBs predicted using the DMB model is easily large enough that an observed distribution of secondary masses or even spectral types in PCEBs can provide an important test of whether magnetic braking is indeed substantially reduced in secondary stars that are fully convective. We discuss briefly the feasibility of such observations.
Integrating Management Information Systems Following Organizational Mergers or Acquisitions

Mergers and acquisitions have become a normal business occurrence for companies large and small. Integration of entities following mergers and acquisitions are often more painful and less successful than they could be. The integration of information technology functions plays a vital role in the ultimate results of such a merger by actualizing product and customer synergies, establishing best practices drawing from the best of each organization, and providing smooth transition to integrated reporting and decision systems. This study proposes to investigate best practice for information technology leaders for integration of functions following mergers and acquisitions, considering outcomes and metrics, pre-merger activities, human resources, culture, learning and other key issues. Results are presented as the distillation of comments extracted from a wide variety of perspectives, organizational situations, and personal reflections by MIS professionals. Additional observations are noted, and several emergent questions aimed at furthering our understanding of this phenomenon are presented.
Sentiment Analysis of Twitter Data : A Survey of Techniques

the advancement of web technology and its growth, there is a huge volume of data present in the web for internet users and a lot of data is generated too. Internet has become a platform for online learning, exchanging ideas and sharing opinions. Social networking sites like Twitter, Facebook, Google+ are rapidly gaining popularity as they allow people to share and express their views about topics, have discussion with different communities, or post messages across the world. There has been lot of work in the field of sentiment analysis of twitter data. This survey focuses mainly on sentiment analysis of twitter data which is helpful to analyze the information in the tweets where opinions are highly unstructured, heterogeneous and are either positive or negative, or neutral in some cases. In this paper, we provide a survey and a comparative analyses of existing techniques for opinion mining like machine learning and lexicon-based approaches, together with evaluation metrics. Using various machine learning algorithms like Naive Bayes, Max Entropy, and Support Vector Machine, we provide research on twitter data streams.We have also discussed general challenges and applications of Sentiment Analysis on Twitter.
A 140mA 90nm CMOS low drop-out regulator with −56dB power supply rejection at 10MHz

A high power supply rejection (PSR) low drop-out (LDO) voltage regulator employing a simple supply ripple cancellation adaptive technique is presented in this paper. The LDO is implemented in 90nm digital CMOS process and occupies an active area of 0.015mm2. The measured PSR is better than −50dB up to 10MHz across the load current range of 140mA with a drop-out voltage of 0.15V. The quiescent current (IQ) varies adaptively from 33µA at no load to 145µA at maximum load with a current efficiency of 99.9%. Load regulation of 6mV for a 140mA step in load current is measured.
Techniques for Multiprocessor Global Schedulability Analysis

The scheduling of sporadic task systems upon multiprocessor platforms is considered, when inter-processor migration is permitted. It is known that current schedulability tests for such systems perform quite poorly when compared to schedulability tests for partitioned scheduling. Limitations of current tests are identified, which may be responsible for the unsatisfactory performance of these tests. A new test that overcomes some of these limitations is proposed and proved correct.
Determination of perchlorate in infant formula by isotope dilution ion chromatography/tandem mass spectrometry

A sensitive and selective isotope dilution ion chromatography/tandem mass spectrometry (ID IC-MS/MS) method was developed and validated for the determination of perchlorate in infant formula. The perchlorate was extracted from infant formula by using 20 ml of methanol and 5 ml of 1% acetic acid. All samples were spiked with 18O4 isotope-labelled perchlorate internal standard prior to extraction. After purification on a graphitised carbon solid-phase extraction column, the extracts were injected into an ion chromatography system equipped with an Ionpac AS20 column for separation of perchlorate from other anions. The presence of perchlorate in samples was quantified by isotope dilution mass spectrometry. Analysis of both perchlorate and its isotope-labelled internal standard was carried out on a Waters Quattro Ultima triple quadrupole mass spectrometer operating in a multiple reaction monitoring (MRM) negative ionisation mode. The method was validated for linearity and range, accuracy, precision, sensitivity, and matrix effects. The limit of quantification (LOQ) was 0.4 µg l−1 for liquid infant formula and 0.95 µg kg−1 for powdered infant formula. The recovery ranged from 94% to 110% with an average of 98%. This method was used to analyse 39 infant formula, and perchlorate concentrations ranging from
African Students’ Identity Negotiation and Relational Conflict Management: Being “Foreign”, Being “Careful”

ABSTRACT International students face challenges associated with identity negotiation as they interact with others who may hold distinctly differing worldviews while operating from a position of cultural dominance. The current study explores how African students negotiate identity in interactions with domestic students within a relational-conflict context. Themes emerging include uncertainty in self-presentation, constrained relationships with domestic students, and avoidance as a response to relational conflict. The emergent concept is that of “being careful”, attributable to African students’ perception of power differences between foreign and domestic students, as well as risk associated with assertive conflict behavior. Theoretical and practical implications are discussed.
Effects of the halo concentration distribution on strong-lensing optical depth and X-ray emission

We use simulated merger trees of galaxy-cluster halos to study the effect of the halo concentration distribution on strong lensing and X-ray emission. Its log-normal shape typically found in simulations favors outliers with high concentration. Since, at fixed mass, more concentrated halos tend to be more efficient lenses, the scatter in the concentration increases the strong-lensing optical depth by <50%. Within cluster samples, mass and concentration have counteracting effects on strong lensing and X-ray emission because the concentration decreases for increasing mass. Selecting clusters by concentration thus has no effect on the lensing cross section. The most efficiently lensing and hottest clusters are typically the least concentrated in samples with a broad mass range. Among cluster samples with a narrow mass range, however, the most strongly lensing and X-ray brightest clusters are typically 10% to 25% more concentrated.
Quasi-orthogonal drawing of planar graphs

Orthogonal drawings of graphs are highly accepted in practice. For planar graphs with vertex degree of at most four, Tamassia gives a polynomial time algorithm which computes a region preserving orthogonal grid embedding with the minimum number of bends. However, the graphs arising in practical applications rarely have bounded vertex degree. In order to cope with general planar graphs, we introduce the quasi–orthogonal drawing model. In this model, vertices are drawn on grid points, and edges follow the grid paths except around vertices of high degree. Furthermore we present an extension of Tamassia’s algorithm that constructs quasi–orthogonal drawings. We compare the drawings to those obtained using related approaches.
Coregulation of host-adapted metabolism and virulence by pathogenic yersiniae

Deciphering the principles how pathogenic bacteria adapt their metabolism to a specific host microenvironment is critical for understanding bacterial pathogenesis. The enteric pathogenic Yersinia species Yersinia pseudotuberculosis and Yersinia enterocolitica and the causative agent of plague, Yersinia pestis, are able to survive in a large variety of environmental reservoirs (e.g., soil, plants, insects) as well as warm-blooded animals (e.g., rodents, pigs, humans) with a particular preference for lymphatic tissues. In order to manage rapidly changing environmental conditions and interbacterial competition, Yersinia senses the nutritional composition during the course of an infection by special molecular devices, integrates this information and adapts its metabolism accordingly. In addition, nutrient availability has an impact on expression of virulence genes in response to C-sources, demonstrating a tight link between the pathogenicity of yersiniae and utilization of nutrients. Recent studies revealed that global regulatory factors such as the cAMP receptor protein (Crp) and the carbon storage regulator (Csr) system are part of a large network of transcriptional and posttranscriptional control strategies adjusting metabolic changes and virulence in response to temperature, ion and nutrient availability. Gained knowledge about the specific metabolic requirements and the correlation between metabolic and virulence gene expression that enable efficient host colonization led to the identification of new potential antimicrobial targets.
Effects of Extracellular Matrix on the Morphology and Behaviour of Rabbit Auricular Chondrocytes in Culture

Isolated chondrocytes dedifferentiate to a fibroblast-like shape on plastic substrata and proliferate extensively, but rarely form nodules. However, when dissociation is not complete and some cartilage remnants are included in the culture, proliferation decreases and cells grow in a reticular pattern with numerous nodules, which occasionally form small cartilage-like fragments. In an attempt to reproduce this stable chondrogenic state, we added a cartilage protein extract, a sugar extract, and hyaluronan to the medium of previously dedifferentiated chondrocytes. When protein extract was added, many cartilaginous nodules appeared. Hyaluronan produced changes in cell phenotype and behaviour, but not nodule formation. Protein extract has positive effects on the differentiation of previously proliferated chondrocytes and permits nodule formation and the extensive production of type-II collagen. A comparison with incompletely dissociated chondrocyte cultures suggests that the presence of some living cells anchored to their natural extracellular matrix provides some important additional factors for the phenotypical stability of chondrocytes on plastic surfaces. In order to elucidate if it is possible that the incidence of apoptosis is related to the results, we also characterized the molecular traits of apoptosis.
Oral Tolerance Induced by Transfer of Food Antigens via Breast Milk of Allergic Mothers Prevents Offspring from Developing Allergic Symptoms in a Mouse Food Allergy Model

We examined whether maternal exposure to food antigens during lactation and maternal allergic status would affect the development of food allergy in offspring. OVA-sensitized or OVA-nonsensitized BALB/c female mice were exposed or unexposed to OVA during lactation. After weaning, their offspring were systemically sensitized twice with OVA and repeatedly given OVA by oral intubation. While 97.1% of the mice breastfed by OVA-nonsensitized and OVA-unexposed mothers developed allergic diarrhea, 59.7% of the mice breastfed by OVA-exposed nonallergic mothers during lactation and 24.6% of the mice breastfed by OVA-exposed allergic mothers during lactation developed food allergy. Furthermore, OVA was detected in breast-milk from OVA-exposed nonallergic mothers during lactation (4.6 ± 0.5 μg/mL). In addition, OVA-specific IgG1 titers were markedly increased in breast milk from allergic mothers (OVA-sensitized and OVA-unexposed mother: 11.0 ± 0.5, OVA-sensitized and OVA-exposed mother: 12.3 ± 0.3). Our results suggest that oral tolerance induced by breast milk-mediated transfer of dietary antigens along with their specific immunoglobulins to offspring leads to antigen-specific protection from food allergy.
Young Children's Comprehension of Temporal Relations in Complex Sentences: The Influence of Memory on Performance

The present study investigated 3‐ to 7‐year‐olds' (N = 91) comprehension of two‐clause sentences containing the temporal connectives before or after. The youngest children used an order of mention strategy to interpret the relation between clauses: They were more accurate when the presentation order matched the chronological order of events: “He ate his lunch, before he played in the garden” (chronological) versus “Before he played in the garden, he ate his lunch” (reverse). Between 4 and 6 years, performance was influenced by a combination of factors that influenced processing load: connective type and presentation order. An independent measure of working memory was predictive of performance. The study concludes that the memory demands of some sentence structures limits young children's comprehension of sentences containing temporal connectives.
Carcinogenesis Colon of Azoxymethane-treated Rats : Correlation with Identification of Mucin-depleted Foci in the Unsectioned Updated Version

We tested the association between aberrant crypt foci (ACF) and tumor induction by feeding azoxymethane-induced rats (15 mg/kg 2, s.c.) with synbiotics (Raftilose Synergy 1, a derivative of inulin, 10% of the diet, along with lactobacilli and bifidobacteria). After 16 weeks of feeding, synbiotics significantly increased ACF multiplicity. On the contrary, after 32 weeks, synbiotics significantly decreased intestinal tumors. When the same unsectioned colon used for ACF determination was stained with high-iron diamine Alcian blue, foci of crypts with scarce or absent mucins were identified. We defined these lesions as mucin-depleted foci (MDF), and they were visible in all azoxymethane-treated rats and correlated with tumor induction (MDF/colon: 8.2 0.9 and 3.8 0.9 in controls and synbiotic-fed rats, respectively, P < 0.01; crypts/MDF: 12.2 2 and 6.4 1 in controls and synbiotic-fed rats, respectively, P < 0.05, means SE, n 7). There were fewer MDF/colon than ACF, and they were histologically more dysplastic than mucinous lesions identified as ACF in high-iron diamine Alcian blue-stained colon. In conclusion, MDF may be premalignant lesions that predict colon carcinogenesis.
An Analysis of the Students' Ability in Using Adverb of Frequency in Simple Present Tense: a Study on the First Year Students of SMA N 7 Padang in Academic Year 2009/2010

The objective of this research was to describe the ability of the first year students of SMA N 7 Padang to use adverb of frequency in simple present tense. The population of this research was the first year students of SMA N 7 Padang in academic year 2009/2010. There were 305 students as members of population. In selecting the sample, the researcher used cluster random sampling. Finally, class X2 that consisted of 36 students was a sample of this research. in technique of collecting data, the researcher used structure test that consisted of 40 items as instrumenataion. Before giving the test to the sample, the test was tried out. Then, the researcher analyzed the data by counting standard deviation (SD) and mean (M). Generally, the result of this research showed that the ability of the first year students of SMA N 7 Padang to use adverb of frequency in simple present tense was moderate. It was proved by the fact that 6 students (16,7%) had high ability, 23 students (63,9%) had moderate ability, and 7 students (19,4%). Specifically, the ability of the students to use adverb of frequency in affirmative sentence of smple present tense was moderate since 25 students (69.4%) had moderate ability. The ability of students to use adverb of frequency in negative sentence of simple present tense was moderate. It was due to the fact that 22 students (61.1%) had moderate ability, the ability of students to use adverb of frequency in interrogative sntece of smple present tense was moderte. it was due to the fact that 16 students (44.4%) had moderate ability. Keywords: Ability, Adverb of Frequency, Present Tense.
Evaluation of the coverage of linked Canadian Community Health Survey and hospital inpatient records.

BACKGROUND Evaluation of the coverage that results from linking routinely collected administrative hospital data with survey data is an important preliminary to undertaking analyses based on the linked file. DATA AND METHODS To evaluate the coverage of the linkage between data from cycle 1.1 of the Canadian Community Health Survey (CCHS) and inpatient hospital data (Health Person-Oriented Information or HPOI), the number of people admitted to hospital according to HPOI was compared with the weighted estimate for CCHS respondents who were successfully linked to HPOI. Differences between HPOI and the linked and weighted CCHS estimate indicated linkage failure and/or undercoverage. RESULTS According to HPOI, from September 2000 through November 2001, 1,572,343 people (outside Quebec) aged 12 or older were hospitalized. Weighted estimates from the linked CCHS, adjusted for agreement to link and plausible health number, were 7.7% lower. Coverage rates were similar for males and females. Provincial rates did not differ from those for the rest of Canada, although differences were apparent for the territories. Coverage rates were significantly lower among people aged 75 or older than among those aged 12 to 74.