Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression and heat‐responsive regulation of a TFIIB homologue from the archaeon Haloferax volcanii

Multiple divergent genes encoding the eukaryal‐like TFIIB (TFB) transcription initiation factor have been identified in the archaeon Haloferax volcanii. Expression of one of these TFB‐encoding genes, referred to here as tfb2, was induced specifically in response to heat shock at the transcription level. A time course for tfb2 induction demonstrated that mRNA levels increased as much as eightfold after 15 min at 60°C. A transcription fusion of the tfb2 promoter region with a stable RNA reporter gene confirmed the heat responsiveness of the tfb2 core promoter, and immunoblot analysis using antibodies generated against a recombinant His‐tagged TFB2 showed that the protein levels of one TFB increased slightly in response to elevated temperatures. An archaeal consensus TATA element (5′‐TTTATA‐3′) was located 110 bp upstream of the translation start site and appeared to be used for both basal and heat shock‐induced expression. The long DNA leader region (79 bp) preceding the predicted AUG translation start codon for TFB2 contained a T‐rich sequence element located 22 bp downstream of the transcription start site. Using an in vivo transcription termination assay, we demonstrated that this T‐rich element can function as a sequence‐dependent transcription terminator, which may serve to downregulate expression of the tfb2 gene under both non‐heat shock and heat shock conditions.
Nicotinic α7 receptor inhibits the acylation stimulating protein-induced production of monocyte chemoattractant protein-1 and keratinocyte-derived chemokine in adipocytes by modulating the p38 kinase and nuclear factor-κB signaling pathways

Obesity is associated with chronic low-grade inflammation, which is characterized by increased infiltration of macrophages into adipose tissue. Acylation stimulating protein (ASP) is an adipokine derived from the immune complement system, which constitutes a link between adipocytes and macrophages, and is involved in energy homeostasis and inflammation. The purpose of the present study was to preliminarily investigate in vitro, whether functional α7nAChR in adipocytes may suppress ASP-induced inflammation and determine the possible signaling mechanism. Studies have reported associations between the expression of α7 nicotinic acetylcholine receptor (α7nAChR) and obesity, insulin resistance and diabetes. Additionally, α7nAChRs are important peripheral mediators of chronic inflammation, which is a key contributor to health problems in obesity. The primary aim of the present study was to evaluate the impact of exogenous ASP and α7nAChR on macrophage infiltration in adipose tissue and to examine the potential underlying molecular mechanism. Western blot analysis revealed that recombinant ASP increased the expression levels of monocyte chemoattractant protein-1 (MCP-1) and keratinocyte-derived chemokine (KC) by 3T3-L1 adipocytes. However, nicotine significantly inhibited the production of ASP-induced cytokines via the stimulation of α7nAChR. It was also found that α7nAChR inhibited the ASP-induced activation of p38 kinase and nuclear factor-κB (NF-κB), and the production of MCP-1 and KC. These data indicated that α7nAChR caused the inhibition of ASP-induced activation of p38 kinase and NF-κB to inhibit the production of MCP-1 and KC.
A Novel Approach to Frame Rate Up-conversion Based on Morphing

This paper proposes a novel frame rate up-conversion(FRUC) algorithm based on morphing. In order to obtain high quality interpolated frames, the proposed algorithm follows prewarping in view morphing to find more accurate matching points, and use three steps to gain dense matching points: coarse matching, error match rejecting and points densifying. Unlike conventional motion compensated interpolation(MCI) algorithm based on block matching motion estimation, the proposed FRUC method uses morphing technique to generate smooth transition between images. This technique overcomes block effect of block matching algorithm(BMA) and provides ability to modeling more complex motion such as rotation and scaling. Experimental results show that the proposed algorithm outperforms conventional block matching MCI algorithm in perceptual quality and can generate smooth and high quality interpolated frames.
THE MENTAL HEALTH OF WAR-WOUNDED REFUGEES: AN 8-YEAR FOLLOW-UP

The complex nature of recent wars and armed conflicts has forced many war-injured persons into exile. To investigate their long-term mental health, three instruments for assessing mental health (HSCL-25, PTSS-10, and a Well-Being scale) were presented to 44 war-wounded refugees from nine different countries 8 years after arrival in Sweden. The prevalence of psychiatric symptoms was high and corresponds to findings in previous studies of refugee patient populations. A lower level of mental health was associated with higher education, unemployment, and poor physical health. The findings suggest a high psychiatric morbidity and a need for psychiatric interventions in this refugee group. Methodological issues to be considered in research on sequels of war traumas are discussed.
Graft Rejection: Immunological Suppression

Transplantation of solid organs is the treatment of choice for most patients with end-stage organ diseases. In the absence of pharmacological immunosuppression, recognition of foreign (allogeneic) histocompatibility proteins expressed on donor cells by the recipient's immune system results in rejection of the transplanted tissue(s). One-year renal transplant survival is now routinely over 90% in most centres, largely the result of improvements in immunosuppressive drugs. In this article, we review commonly used immunosuppressive medications and discuss their pharmacological modes of action. Given that long-term graft outcomes remain poor despite improvements in early transplant survival, we discuss, in addition, novel experimental strategies for the induction of tolerance to transplanted tissues that have translational relevance to human organ recipients. Key Concepts: The major limitation of solid organ transplantation is long-term immunosuppression which either results in excessive mortality and morbidity or is insufficient to prevent chronic rejection of transplanted tissues. An understanding of transplant rejection and pharmacological targets of immunosuppression is key to devising novel strategies of preventing rejection by inducing transplant tolerance. Keywords: transplantation; rejection; tissue typing; immunosuppression
Capacity growth for CDMA system: multiple sectors and multiple carriers deployment

Due to the increasing acceptability of CDMA, all infrastructure/service providers are accelerating their investigation to map out strategies to handle the increasing capacity demand. In the AMPS/TDMA technology, cell splitting has been one of the standard practices to improve the frequency reuse factor in order to increase capacity. Furthermore, dynamic channel/resource allocation has been demonstrated to provide capacity relief in localized hot spots. In the CDMA technology, however, since the conventional AMPS/TDMA frequency planning is not required, there are new strategies to increase capacity. Previously, Wong and Prabhu (see IEEE VTC, p.1177-81, 1997) had investigated the optimum sectorization scheme for a CDMA base station to provide higher capacity. Multiple carriers deployment provides another option. From a capacity standpoint, even an un-orchestrated multiple carriers deployment multiplies the capacity of the network. With an intelligent resource allocation scheme, the traffic loading among multiple carriers can be intelligently managed to increase the trunking efficiency of the network. It results in much higher aggregated Erlang capacity and better system redundancy.
DYNAMIC BUCKLING OF SHALLOW SPHERICAL CAPS SUBJECTED TO A NEARLY AXISYMMETRIC STEP PRESSURE LOAD

The behavior of shallow spherical caps subjected to axisymmetric and nearly axisymmetric step pressure loads is examined using a digital computer program for the geometrically nonlinear analysis of arbitrarily loaded shells of revolution. A criterion for dynamic buckling under the nearly axisymmetric load is proposed and critical buckling pressures are determined for both loading conditions for a large range of cap sizes. The axisymmetric results either compare well with, or are somewhat higher than, previously published theoretical results. For most of the cap sizes rhe buckling pressures from the asymmetric analysis are new results. A numerical convergence study is conducted to verify the accuracy of the results.
Intrinsic Voltage Dependence and Ca2+ Regulation of mslo Large Conductance Ca-activated K+ Channels

The kinetic and steady-state properties of macroscopic mslo Ca-activated K+ currents were studied in excised patches from Xenopus oocytes. In response to voltage steps, the timecourse of both activation and deactivation, but for a brief delay in activation, could be approximated by a single exponential function over a wide range of voltages and internal Ca2+ concentrations ([Ca]i). Activation rates increased with voltage and with [Ca]i, and approached saturation at high [Ca]i. Deactivation rates generally decreased with [Ca]i and voltage, and approached saturation at high [Ca]i. Plots of the macroscopic conductance as a function of voltage (G-V) and the time constant of activation and deactivation shifted leftward along the voltage axis with increasing [Ca]i. G-V relations could be approximated by a Boltzmann function with an equivalent gating charge which ranged between 1.1 and 1.8 e as [Ca]i varied between 0.84 and 1,000 μM. Hill analysis indicates that at least three Ca2+ binding sites can contribute to channel activation. Three lines of evidence indicate that there is at least one voltage-dependent unimolecular conformational change associated with mslo gating that is separate from Ca2+ binding. (a) The position of the mslo G-V relation does not vary logarithmically with [Ca]i. (b) The macroscopic rate constant of activation approaches saturation at high [Ca]i but remains voltage dependent. (c) With strong depolarizations mslo currents can be nearly maximally activated without binding Ca2+. These results can be understood in terms of a channel which must undergo a central voltage-dependent rate limiting conformational change in order to move from closed to open, with rapid Ca2+ binding to both open and closed states modulating this central step.
A hierarchical fair service curve algorithm for link-sharing, real-time and priority services

In this paper, we study hierarchical resource management models and algorithms that support both link-sharing and guaranteed real-time services with decoupled delay (priority) and bandwidth allocation. We extend the service curve based QoS model, which defines both delay and bandwidth requirements of a class, to include fairness, which is important for the integration of real-time and hierarchical link-sharing services. The resulting Fair Service Curve link-sharing model formalizes the goals of link-sharing and real-time services and exposes the fundamental tradeoffs between these goals. In particular, with decoupled delay and band-width allocation, it is impossible to simultaneously provide guaranteed real-time service and achieve perfect link-sharing. We propose a novel scheduling algorithm called Hierarchical Fair Service Curve (H-FSC) that approximates the model closely and efficiently. The algorithm always guarantees the performance for leaf classes, thus ensures real-time services, while minimizing the discrepancy between the actual services provided to the interior classes and the services defined by the Fair Service Curve link-sharing model. We have implemented the H-FSC scheduler in the NetBSD environment. By performing simulation and measurement experiments, we evaluate the link-sharing and real-time performances of H-FSC, and determine the computation over-head.
FeNO as a Marker of Airways Inflammation: The Possible Implications in Childhood Asthma Management

The aim of this study was to verify FeNO usefulness, as a marker of bronchial inflammation, in the assessment of therapeutic management of childhood asthma. We performed a prospective 1-year randomized clinical trial evaluating two groups of 32 children with allergic asthma: “GINA group”, in which therapy was assessed only by GINA guidelines and “FeNO group”, who followed a therapeutic program assessed also on FeNO measurements. Asthma Severity score (ASs), Asthma Exacerbation Frequency (AEf), and Asthma Therapy score (ATs) were evaluated at the start of the study (T1), 6 months (T2), and 1 year after (T3). ASs and AEf significantly decreased only in the FeNO group at times T2 and T3 (p[T1-T2] = 0.0001, and p[T1-T3] = 0.01; p[T1-T2] = 0.0001; and p[T1-T3] < 0.0001, resp.). After six months of follow-up, we found a significant increase of patients under inhaled corticosteroid and/or antileukotrienes in the GINA group compared to the FeNO group (P = .02). Our data show that FeNO measurements, might be a very useful additional parameter for management of asthma, which is able to avoid unnecessary inhaled corticosteroid and antileukotrienes therapies, however, mantaining a treatment sufficient to obtain a meaningful improvement of asthma.
Stakeholder participation in the development of an electronic medical record system in Malawi

In this paper we are concerned with stakeholders' participation in the development of an Electronic Medical Record (EMR) system for health facilities in Malawi, Africa. We draw on insights gained during the process of an Action Research project, which involved different stakeholders. We examine the different roles and forms of participation of these stakeholders. Through this, we illustrate how participation changes over time and co-evolves with the progress of the project. Our analysis also reveals that, in rural low-resource settings, expected end-users of EMR systems do not always have the health domain knowledge or expertise to effectively participate in such design projects. Therefore, participation of managers and other health domain experts is essential in order to supplement users' limited specialized knowledge of the domain.
Cell Wall Metabolism in Ripening Fruit (VI. Effect of the Antisense Polygalacturonase Gene on Cell Wall Changes Accompanying Ripening in Transgenic Tomatoes)

Cell walls of tomato (Lycopersicon esculentum Mill.) fruit, prepared so as to minimize residual hydrolytic activity and autolysis, exhibit increasing solubilization of pectins as ripening proceeds, and this process is not evident in fruit from transgenic plants with the antisense gene for polygalacturonase (PG). A comparison of activities of a number of possible cell wall hydrolases indicated that antisense fruit differ from control fruit specifically in their low PG activity. The composition of cell wall fractions of mature green fruit from transgenic and control (wild-type) plants were indistinguishable except for trans-1,2-diaminocyclohexane-N,N,N[prime],N[prime]-tetraacetic acid (CDTA)-soluble pectins of transgenic fruit, which had elevated levels of arabinose and galactose. Neutral polysaccharides and polyuronides increased in the water-soluble fraction of wild-type fruit during ripening, and this was matched by a decline in Na2CO3-soluble pectins, equal in magnitude and timing. This, together with compositional analysis showing increasing galactose, arabinose, and rhamnose in the water-soluble fraction, mirrored by a decline of these same residues in the Na2CO3-soluble pectins, suggests that the polyuronides and neutral polysaccharides solubilized by PG come from the Na2CO3-soluble fraction of the tomato cell wall. In addition to the loss of galactose from the cell wall as a result of PG activity, both antisense and control fruit exhibit an independent decline in galactose in both the CDTA-soluble and Na2CO3-soluble fractions, which may play a role in fruit softening.
Antiwear and Extreme Pressure Properties of Nanofluids for Industrial Applications

This study investigates the effect of CuO, TiO2, Al2O3, and multiwalled nanotube (MWNT) nanoparticles at various treat rates on the tribological properties, namely, wear, coefficient of friction (COF), and pressure of seizure (poz), of metalworking fluids during lubricating processes in diverse industrial applications. Results are reported based on two methods: wear scar diameter (WSD) and COF by ASTM D5183 and poz by the Institute for Sustainable Technologies–National Research Institute (ITEePib) Polish method for testing lubricants under scuffing conditions. Results showed significant improvements with small filler concentrations of nanoparticles. CuO nanofluids showed a diminishment of 86% for WSD at 0.01 wt%, whereas TiO2 resulted in an increase in poz of up to ∼250% at 0.05 wt% compared to pure conventional fluid.
Understanding the intentions of users to ‘stick’ to social networking sites: a case study in Taiwan

Social networking sites (SNSs) are the most popular social platforms for developing personal networks. They provide multiple interactive functions for users to create and use large social networks. To determine why people exhibit ‘stickiness’ to SNSs, this study uses the uses and gratifications theory as an underlying structure and builds the research model with factors of motivational needs and interactivity. Our results showed that social needs, information needs, human–message interaction, and human–human interaction are crucial factors that affect the ‘stickiness’ of users to SNSs. The implications of these findings are presented in this paper.
Structure and transport mechanism of the sodium/proton antiporter MjNhaP1

Sodium/proton antiporters are essential for sodium and pH homeostasis and play a major role in human health and disease. We determined the structures of the archaeal sodium/proton antiporter MjNhaP1 in two complementary states. The inward-open state was obtained by x-ray crystallography in the presence of sodium at pH 8, where the transporter is highly active. The outward-open state was obtained by electron crystallography without sodium at pH 4, where MjNhaP1 is inactive. Comparison of both structures reveals a 7° tilt of the 6 helix bundle. 22Na+ uptake measurements indicate non-cooperative transport with an activity maximum at pH 7.5. We conclude that binding of a Na+ ion from the outside induces helix movements that close the extracellular cavity, open the cytoplasmic funnel, and result in a ∼5 Å vertical relocation of the ion binding site to release the substrate ion into the cytoplasm. DOI: http://dx.doi. org/10.7554/eLife.03583. 001
Effectiveness of Preacclimatization Strategies for High-Altitude Exposure

Acute mountain sickness (AMS) and large decrements in endurance exercise performance occur when unacclimatized individuals rapidly ascend to high altitudes. Six altitude and hypoxia preacclimatization strategies were evaluated to determine their effectiveness for minimizing AMS and improving performance during altitude exposures. Strategies using hypobaric chambers or true altitude were much more effective overall than those using normobaric hypoxia (breathing, <20.9% oxygen).
Correlations between islet autoantibody specificity and the SLC30A8 genotype with HLA-DQB1 and metabolic control in new onset type 1 diabetes

We hypothesised that the correlation between autoantibody specificity for the ZnT8 Arg325Trp isoforms and the type 2 diabetes-associated rs13266634 may affect β-cell function at type 1 diabetes (T1D) onset. To study this, we tested 482 newly diagnosed diabetic probands and 478 healthy siblings from the Danish population-based T1D registry for autoantibodies to ZnT8 (ZnT8A) in addition to GAD65 and IA-2. The prevalence and titres of autoantibodies were correlated with genotypes for rs13266634 and HLA-DQB1, age at diagnosis (AAD) and insulin dose-adjusted HbA1c (IDAA1c), as a proxy for residual β-cell function. We replicated the correlation between rs13266634 genotypes and specificity for the ZnT8-Argenine (ZnT8R) and ZnT8-Tryptophan (ZnT8W) isoforms previously reported. ZnT8A overlapped substantially with autoantibodies to glutamate decarboxylase 65 (GADA) and IA-2 (IA-2A) and correlated significantly with IA-2A prevalence (p < 2e-16). No effect on IDAA1c was demonstrated for ZnT8A or rs13266634. We found a correlation between ZnT8R positivity and HLA-DQB1*0302 genotypes (p = 0.016), which has not been shown previously. Furthermore, significantly lower ZnT8R and GADA prevalence and titres was found among probands with AAD < 5 years (prevalence: p = 0.004 and p = 0.0001; titres: p = 0.002 and p = 0.001, respectively). The same trend was observed for IA-2A and ZnT8W; however, the difference was non-significant. Our study confirms ZnT8 as a major target for autoantibodies at disease onset in our Danish T1D cohort of children and adolescents, and we have further characterised the relationship between autoantibody specificity for the ZnT8 Arg325Trp epitopes and rs13266634 in relation to established autoantibodies, AAD, measures of β-cell function and HLA-DQB1 genotypes in T1D.
Multiple Notch signaling events control Drosophila CNS midline neurogenesis, gliogenesis and neuronal identity

The study of how transcriptional control and cell signaling influence neurons and glia to acquire their differentiated properties is fundamental to understanding CNS development and function. The Drosophila CNS midline cells are an excellent system for studying these issues because they consist of a small population of diverse cells with well-defined gene expression profiles. In this paper, the origins and differentiation of midline neurons and glia were analyzed. Midline precursor (MP) cells each divide once giving rise to two neurons; here, we use a combination of single-cell gene expression mapping and time-lapse imaging to identify individual MPs, their locations, movements and stereotyped patterns of division. The role of Notch signaling was investigated by analyzing 37 midline-expressed genes in Notch pathway mutant and misexpression embryos. Notch signaling had opposing functions: it inhibited neurogenesis in MP1,3,4 and promoted neurogenesis in MP5,6. Notch signaling also promoted midline glial and median neuroblast cell fate. This latter result suggests that the median neuroblast resembles brain neuroblasts that require Notch signaling, rather than nerve cord neuroblasts, the formation of which is inhibited by Notch signaling. Asymmetric MP daughter cell fates also depend on Notch signaling. One member of each pair of MP3-6 daughter cells was responsive to Notch signaling. By contrast, the other daughter cell asymmetrically acquired Numb, which inhibited Notch signaling, leading to a different fate choice. In summary, this paper describes the formation and division of MPs and multiple roles for Notch signaling in midline cell development, providing a foundation for comprehensive molecular analyses.