Miracle-dz/UniFAR-FTUs huggingface dataset:
Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice.

OBJECTIVE The present work was undertaken to determine the effects of Amaranthus roxburghianus Nevski. (Amaranthaceae) root alone and in combination with piperine in treating ulcerative colitis (UC) in mice. METHODS Swiss albino mice were divided into seven groups (n = 6). Standard group received prednisolone (5 mg/kg, intraperitoneally). Treatment groups received hydroalcoholic extract of roots of A. roxburghianus (50 and 100 mg/kg, per oral) and a combination of hydroalcoholic extract of roots of A. roxburghianus (50 and 100 mg/kg, per oral) and piperine (5 mg/kg, per oral). Ulcer index, colitis severity, myeloperoxidase (MPO), malondialdehyde and glutathione were estimated from blood and tissue. Column chromatography of the extract was done and purified fractions were analyzed by gas chromatography-mass spectroscopy (GC-MS). RESULTS Treatment with the combination of hydroalcoholic extract of A. roxburghianus and piperine showed minimal ulceration, hemorrhage, necrosis and leucocyte infiltration by histopathological observation. Acetic acid increased MPO levels in blood and colon tissue to 355 U/mL and 385 U/mg, respectively. The combination of hydroalcoholic extract of A. roxburghianus (100 mg/kg) and piperine (5 mg/kg) significantly decreased MPO in blood and tissue to 182 U/mL and 193 U/mg, respectively (P < 0.05). Similarly, this combination significantly reduced malondialdehyde levels and increased glutathione levels in blood and tissue. Various phytoconstituents were detected by GC-MS. CONCLUSION The combination of hydroalcoholic extract of A. roxburghianus and piperine is effective in the treatment of UC and the effects are comparable with the standard drug prednisolone. 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, eugenol and benzene, and 1-(1,5-dimethyl-4-hexenyl)-4-methyl are reported having analgesic, anti-inflammatory, and antioxidant properties; they may play a role in the biological activity of A. roxburghianus root.
Incentives and Creativity: Evidence from the Academic Life Sciences

Despite its presumed role as an engine of economic growth, we know surprisingly little about the drivers of scientific creativity. In this paper, we exploit key differences across funding streams within the academic life sciences to estimate the impact of incentives on the rate and direction of scientific exploration. Specifically, we study the careers of investigators of the Howard Hughes Medical Institute (HHMI), which tolerates early failure, rewards long-term success, and gives its appointees great freedom to experiment; and grantees from the National Institute of Health, which are subject to short review cycles, pre-defined deliverables, and renewal policies unforgiving of failure. Using a combination of propensity-score weighting and difference-in-differences estimation strategies, we find that HHMI investigators produce high- impact papers at a much higher rate than a control group of similarly-accomplished NIH-funded scientists. Moreover, the direction of their research changes in ways that suggest the program induces them to explore novel lines of inquiry.
Evaluating Amyloid-β Oligomers in Cerebrospinal Fluid as a Biomarker for Alzheimer’s Disease

The current study evaluated amyloid-β oligomers (Aβo) in cerebrospinal fluid as a clinical biomarker for Alzheimer’s disease (AD). We developed a highly sensitive Aβo ELISA using the same N-terminal monoclonal antibody (82E1) for capture and detection. CSF samples from patients with AD, mild cognitive impairment (MCI), and healthy controls were examined. The assay was specific for oligomerized Aβ with a lower limit of quantification of 200 fg/ml, and the assay signal showed a tight correlation with synthetic Aβo levels. Three clinical materials of well characterized AD patients (n = 199) and cognitively healthy controls (n = 148) from different clinical centers were included, together with a clinical material of patients with MCI (n = 165). Aβo levels were elevated in the all three AD-control comparisons although with a large overlap and a separation from controls that was far from complete. Patients with MCI who later converted to AD had increased Aβo levels on a group level but several samples had undetectable levels. These results indicate that presence of high or measurable Aβo levels in CSF is clearly associated with AD, but the overlap is too large for the test to have any diagnostic potential on its own.
Optimizing Immunomarking Systems and Development of a New Marking System Based on Wheat

Abstract Immunomarking systems used to track large-scale movement patterns of insects are highly dependent on the efficiency of the enzyme linked imunosorbent assay (ELISA) reaction and logistical factors (e.g. concentration of marker applied, ability of the marker to wet the insect cuticle, and trapping methods). This paper examines ways to increase ELISA efficiency and mediate logistical factors, and provides information on a new immunomarking protein based on wheat gluten. The present studies on improving efficiency of the ELISA reactions showed that specially treated microplate surfaces were needed for soymilk and gluten assays, but not for egg albumin and casein assays. Sample dilution was investigated and was found to improve the signal/noise (S/N) ratio for the albumin and casein assays, but S/N ratios for the gluten and soymilk assays were less sensitive. However, for all assays, marked specimens were still detectable even with dilutions down to 6% of the original sample, which would allow more tests to be run on the same initial sample volume. For the logistical factors, these studies showed that marking of an insect by having it walk across a dried residue could be virtually eliminated for the casein and soymilk assays when the concentration applied was reduced to < 4%, but residues of 0.125% egg that had been aged in the field seven days still marked 37.5% of test insects placed on the residues. Also, the adjuvant Sylgard® 309 used at 80 ppm enhanced wetting of the insect cuticle and had little or no effect on the ELISA reaction, but the wetting agents R-11 and Silwet® L-77 were much more likely to negatively affect ELISA performance. Five different trapping adhesives were also evaluated and found to reduce ELISA efficiency 38–45% for the casein assay and 61–78% for the soymilk assay, while the albumin and gluten assays were unaffected. The information provided in this paper can be used to help correct for inherent differences in marking efficiency of the different proteins by manipulation of sample preparation, adjuvants, and concentrations applied.
Modeling of Dispersive Transport in the Context of Negative Bias Temperature Instability

Negative bias temperature instability (NBTI) is one of the most serious reliability concerns for highly scaled pMOSFETs. It is most commonly interpreted by some form of reaction-diffusion (RD) model, which assumes that some hydrogen species is released from previously passivated interface defects, which then diffuses into the oxide. It has been argued, however, that hydrogen motion in the oxide is trap-controlled, resulting in dispersive transport behavior. This defect-controlled transport modifies the characteristic exponent in the power-law that describes the threshold-voltage shift. However, previously published models are contradictory and both an increase and a decrease in the power-law exponent have been reported. We clarify this discrepancy by identifying the boundary condition which couples the transport equations to the electro-chemical reaction at the interface as the crucial component of the physically-based description
New multigrid smoothers for the Oseen problem

We investigate the performance of smoothers based on the Hermitian/skew‐Hermitian (HSS) and augmented Lagrangian (AL) splittings applied to the Marker‐and‐Cell (MAC) discretization of the Oseen problem. Both steady and unsteady flows are considered. Local Fourier analysis and numerical experiments on a two‐dimensional lid‐driven cavity problem indicate that the proposed smoothers result in h‐independent convergence and are fairly robust with respect to the Reynolds number. A direct comparison shows that the new smoothers compare favorably to coupled smoothers of Braess–Sarazin type, especially in terms of scaling for increasing Reynolds number. Copyright © 2010 John Wiley & Sons, Ltd.
Increased fetuin-A concentrations in impaired glucose tolerance with or without nonalcoholic fatty liver disease, but not impaired fasting glucose.

CONTEXT Fetuin-A, a liver-derived glycoprotein that impairs insulin signaling, is associated with nonalcoholic fatty liver disease (NAFLD), diabetes, and the risk of cardiovascular diseases. Both prediabetes and NAFLD are associated with increased cardiovascular risk, and their concurrence significantly impairs hepatic and adipose tissue insulin sensitivity. OBJECTIVE Our objective was to investigate the relationship between serum fetuin-A levels and prediabetes in subjects with or without NAFLD. DESIGN This was a cross-sectional case-control study. PATIENTS A total of 510 age- and sex-matched subjects with normal glucose tolerance (NGT), impaired fasting glucose (IFG), and impaired glucose tolerance (IGT) with or without NAFLD were recruited. Each subject was assessed by abdominal ultrasound to diagnose NAFLD. MAIN OUTCOME MEASURES Serum fetuin-A concentrations were compared between groups. The association with clinico-metabolic parameters was examined. RESULTS The presence of NAFLD significantly increases fetuin-A levels in subjects with NGT and prediabetes. As compared with NGT, IGT, but not IFG, significantly increases fetuin-A levels in subjects with or without NAFLD. Serum fetuin-A concentrations were positively related to postload 2-h glucose, body mass index, triglyceride, and homeostasis model assessment of insulin resistance but negatively associated with age, high-density lipoprotein cholesterol, and adiponectin. In multiple regression analysis, age, IGT vs. NGT, and IGT with NAFLD vs. NGT were independently associated with fetuin-A levels after adjustment for cardiovascular risk factors and adiponectin. CONCLUSIONS IGT with or without NAFLD was independently associated with fetuin-A levels after adjustment for cardiometabolic risk factors. The elevated fetuin-A levels could have a clinical implication in the increased cardiovascular risk and insulin resistance associated with NAFLD and IGT.
Milk Consumption and Mortality from All Causes, Cardiovascular Disease, and Cancer: A Systematic Review and Meta-Analysis

Results from epidemiological studies of milk consumption and mortality are inconsistent. We conducted a systematic review and meta-analysis of prospective studies assessing the association of non-fermented and fermented milk consumption with mortality from all causes, cardiovascular disease, and cancer. PubMed was searched until August 2015. A two-stage, random-effects, dose-response meta-analysis was used to combine study-specific results. Heterogeneity among studies was assessed with the I2 statistic. During follow-up periods ranging from 4.1 to 25 years, 70,743 deaths occurred among 367,505 participants. The range of non-fermented and fermented milk consumption and the shape of the associations between milk consumption and mortality differed considerably between studies. There was substantial heterogeneity among studies of non-fermented milk consumption in relation to mortality from all causes (12 studies; I2 = 94%), cardiovascular disease (five studies; I2 = 93%), and cancer (four studies; I2 = 75%) as well as among studies of fermented milk consumption and all-cause mortality (seven studies; I2 = 88%). Thus, estimating pooled hazard ratios was not appropriate. Heterogeneity among studies was observed in most subgroups defined by sex, country, and study quality. In conclusion, we observed no consistent association between milk consumption and all-cause or cause-specific mortality.
Eigenspace-based recognition of faces: comparisons and a new approach

Different eigenspace-based approaches have been proposed for the recognition of faces. They differ mostly in the kind of projection method used and in the similarity matching criterion employed. A first goal of this paper is to present a comparison between some of these different approaches. A second goal is to outline an adaptive, neural-based security access control system.
An exploration of modifiable health associated risk factors within a cohort of undergraduate nursing students

Abstract So-called diseases of affluence, otherwise known as ‘lifestyle diseases’, are attributed to modifiable risk factors that are influenced by lifestyle and personal behaviour. Leading by example is an important way for public health principles to be communicated. In the university context, students of nursing can become aware of the challenge to integrate and apply health principles in their own life so that they become responsible health leaders in the community. The aim of this study was to explore the incidence of a number of behaviour-associated health risk factors within a group of undergraduate nursing students. Ninety-four students participated in the study. Seventy-seven students (82%) reported the presence of at least one modifiable health risk factor. Forty-four percent of respondents were either overweight or obese. Further research to explore whether a health promoting curriculum encourages nursing students to internalise/apply health knowledge to their own lives is recommended. A campaign of public health might be useful within the university community to educate students about risk factors and healthy living.
Inflammatory Responses to Migrating Brugia pahangi Third-Stage Larvae

ABSTRACT Despite being central to parasite establishment and subsequent host pathological and immunologic responses, host-parasite interactions during early third-stage filarial larva (L3) migration are poorly understood. These studies aimed to define early tissue migration of Brugia pahangi L3 in the gerbil (Meriones unguiculatus) and measure host cellular responses during this period. Gerbils were intradermally inoculated in the hind limb with 100 B. pahangi L3, and necropsies were performed at various times. At 3 h, most L3 (96.3%) were recovered from tissues associated with the infection site, with marked L3 migration occurring by 24 h. Larvae were dispersed throughout the lymphatics at 7 days postinfection (dpi), and at 28 dpi, most parasites were recovered from the spermatic cord lymphatics. Parasites were identified histologically at all time points. Inflammatory cells, primarily neutrophils, were frequently observed around larvae in the dermis and muscle near the injection site at 3 h and 24 h. Levels of interleukin-6 (IL-6) and tumor necrosis factor-α mRNA peaked at 3 h in all tissues, with IL-6 levels also high in the spleen at 28 dpi. Levels of IL-4 mRNA were elevated in all tissues at 28 dpi. These observations demonstrate that L3 migrate quickly through various tissues and into lymph nodes in a predictable pattern. Migrating L3 induce an early acute inflammatory response that is modulated as parasites establish in the lymphatics. Polarization of the host response towards a dominant Th2-like profile is present at 7 dpi and is well established by 28 dpi in this permissive host.
Textiles with gallic acid microspheres: in vitro release characteristics

Abstract The aim of this study was to demonstrate the skin penetration of an antioxidant, gallic acid (GA), encapsulated in poly-ε-caprolactone (PCL) microspheres and applied onto textile fabrics, by a specific in vitro percutaneous absorption methodology. Two techniques (particle size distribution and FTIR) were used to characterise the microspheres obtained. The amount of GA-loaded microspheres present in the biofunctional textiles was established before their use as a textile drug delivery system. More absorption and desorption of microspheres with GA for the polyamide fabric were found in comparison with cotton fabric. The percutaneous absorption results indicated that the skin penetration of GA released from PCL-microspheres that were applied directly to the skin was higher than when GA was embedded within biofunctional textiles, in conclusion, an interesting reservoir effect may be promoted when biofunctional textiles were used.
The Lifted Newton Method and Its Application in Optimization

We present a new “lifting” approach for the solution of nonlinear optimization problems (NLPs) that have objective and constraint functions with intermediate variables. Introducing these as additional degrees of freedom into the original problem, combined with adding suitable new constraints to ensure equivalence of the problems, we propose to solve this augmented system instead of the original system by a Newton-type method. This often offers advantages in terms of convergence rates and region of attraction. The main contribution of this article is an efficient algorithmic trick to generate the quantities needed for a Newton-type method on the augmented (“lifted”) system with (a) almost no additional computational cost per iteration compared to a nonlifted Newton method, and (b) with negligible programming burden. We derive lifted schemes for Newton's method, as well as for constrained Gauss-Newton and adjoint based sequential quadratic programming (SQP) methods, and show equivalence of the new efficiently lifted approaches with “full-space” lifted Newton iterations. We establish conditions on the intermediate functions that imply faster local quadratic convergence for lifted versus nonlifted Newton iterations, a phenomenon often observed in practice but not yet explained theoretically. Finally, we compare numerically the behavior of the lifted approach with the nonlifted approach on several test problems, including a large scale example with 27 million intermediate variables. The algorithms and examples are available as open-source code in the C++ package LiftOpt.
Repeating Words in Spontaneous Speech

Speakers often repeat the first word of major constituents, as in, "I uh I wouldn't be surprised at that. " Repeats like this divide into four stages: an initial commitment to the constituent (with "I"); the suspension of speech; a hiatus in speaking (filled with "uh"); and a restart of the constituent ("I wouldn't."). An analysis of all repeated articles and pronouns in two large corpora of spontaneous speech shows that the four stages reflect different principles. Speakers are more likely to make a premature commitment, immediately suspending their speech, as both the local constituent and the constituent containing it become more complex. They plan some of these suspensions from the start as preliminary commitments to what they are about to say. And they are more likely to restart a constituent the more their stopping has disrupted its delivery. We argue that the principles governing these stages are general and not specific to repeats.
Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl

As the major mechanism of plant growth and morphogenesis, cell elongation is controlled by many hormonal and environmental signals. How these signals are coordinated at the molecular level to ensure coherent cellular responses remains unclear. In this study, we illustrate a molecular circuit that integrates all major growth-regulating signals, including auxin, brassinosteroid, gibberellin, light, and temperature. Analyses of genome-wide targets, genetic and biochemical interactions demonstrate that the auxin-response factor ARF6, the light/temperature-regulated transcription factor PIF4, and the brassinosteroid-signaling transcription factor BZR1, interact with each other and cooperatively regulate large numbers of common target genes, but their DNA-binding activities are blocked by the gibberellin-inactivated repressor RGA. In addition, a tripartite HLH/bHLH module feedback regulates PIFs and additional bHLH factors that interact with ARF6, and thereby modulates auxin sensitivity according to developmental and environmental cues. Our results demonstrate a central growth-regulation circuit that integrates hormonal, environmental, and developmental controls of cell elongation in Arabidopsis hypocotyl. DOI: http://dx.doi. org/10.7554/eLife.03031. 001
On the statistical properties of the F-measure

The F-measure - the number of distinct test cases to detect the first program failure - is an effectiveness measure for debug testing strategies. We show that for random testing with replacement, the F-measure is distributed according to the geometric distribution. A simulation study examines the distribution of two adaptive random testing methods, to study how closely their sampling distributions approximate the geometric distribution, revealing that in the worst case scenario, the sampling distribution for adaptive random testing is very similar to random testing. Our results have provided an answer to a conjecture that adaptive random testing is always a more effective alternative to random testing, with reference to the F-measure. We consider the implications of our findings for previous studies conducted in the area, and make recommendations to future studies.
Occurrence of Virulence Genes Associated with Diarrheagenic Escherichia coli Isolated from Raw Cow’s Milk from Two Commercial Dairy Farms in the Eastern Cape Province, South Africa

Escherichia coli remains a public health concern worldwide as an organism that causes diarrhea and its reservoir in raw milk may play an important role in the survival and transport of pathogenic strains. Diarrheagenic E. coli strains are diverse food-borne pathogens and causes diarrhea with varying virulence in humans. We investigated the prevalence of pathogenic E. coli in raw milk from two commercial dairy farms. Four hundred raw milk samples, 200 from each dairy farm, were screened for the presence of fliCH7, eagR, ial, eagg, lt, and papC genes. In dairy farm A, 100 E. coli were identified based on culture, oxidase and Gram staining, while 88 isolates from dairy farm B were identified in the same manner. Gene detection showed fliCH7 27 (54%) to be the highest gene detected from farm A and lt 2 (4%) to be the lowest. The highest gene detected in dairy farm B was fliCH7 16 (43.2%) and papC 1 (2.7%) was the least. The amplification of pathogenic genes associated with diarrheagenic E. coli from cows’ raw milk demonstrates that potentially virulent E. coli strains are widely distributed in raw milk and may be a cause of concern for human health.
Robust semi-coarsening multilevel preconditioning of biquadratic FEM systems

AstractWhile a large amount of papers are dealing with robust multilevel methods and algorithms for linear FEM elliptic systems, the related higher order FEM problems are much less studied. Moreover, we know that the standard hierarchical basis two-level splittings deteriorate for strongly anisotropic problems. A first robust multilevel preconditioner for higher order FEM systems obtained after discretizations of elliptic problems with an anisotropic diffusion tensor is presented in this paper. We study the behavior of the constant in the strengthened CBS inequality for semi-coarsening mesh refinement which is a quality measure for hierarchical two-level splittings of the considered biquadratic FEM stiffness matrices. The presented new theoretical estimates are confirmed by numerically computed CBS constants for a rich set of parameters (coarsening factor and anisotropy ratio). In the paper we consider also the problem of solving efficiently systems with the pivot block matrices arising in the hierarchical basis two-level splittings. Combining the proven uniform estimates with the theory of the Algebraic MultiLevel Iteration (AMLI) methods we obtain an optimal order multilevel algorithm whose total computational cost is proportional to the size of the discrete problem with a proportionality constant independent of the anisotropy ratio.