Miracle-dz/UniFAR-FTUs huggingface dataset:
Metachronal propagation of motoneurone burst activation in isolated spinal cord of newborn rat

Adequate locomotor and postural activity in mammals results from the coordinated activation of assemblies of spinal cord networks. In order to assess the global functioning of spinal circuitry, multisite recordings were made from an isolated spinal cord preparation of the newborn rat. Motor activity, elicited in a disinhibited network by bath‐applying strychnine (glycinergic blocker) and bicuculline (GABAergic blocker), consisted of slow spontaneous bursting. Under these conditions, the recorded bursts were coordinated in 1: 1 relationships at all segmental levels. For each cycle, a leading segment initiated the activity that then propagated in a metachronal way through adjacent segments along the length of spinal cord. There was both regional non‐linearity and directional asymmetry in this burst propagation: motor bursts propagated most rapidly in the thoracic spinal cord and the rostro‐caudal wave travelled faster than the caudo‐rostral one. Propagation involved both long projecting fibres and local intersegmental connections. These results suggest that the mammalian spinal cord contains propriospinal pathways subserving a metachronal transmission of motor information and that normally it may be involved in coordinating various parts of the body. The simple model developed here could be useful in unravelling more general mechanisms of neuronal circuit coupling.
Molecular and functional characterization of eight novel GAA mutations in Italian infants with Pompe disease

We characterized 29 unrelated patients presenting with the severe form of Pompe disease (Glycogen Storage Disease Type II, acid maltase deficiency) and identified 26 pathogenic mutations divided over 28 different genotypes. Among the eight new mutations, five were exonic point mutations (c.572A>G, c.1124G>T, c.1202A>G, c.1564C>G and c.1796C>A) leading to codon changes (p.Y191C, p.R375L, p.Q401R, p.P522A and p.S599Y); two were intronic point mutations (c.‐32‐3C>A and c.1636+5G>C) affecting mRNA processing; one was a single base deletion (c.742delC) generating a truncated protein (p.L248PfsX20). A comprehensive evaluation, based on different methodological approaches, confirmed the detrimental effect of the eight mutations on the protein and its function. Structural alterations potentially induced by the five missense mutations were also predicted through visual inspection of the atomic model of the GAA protein, in terms of both function and spatial orientation of specific residues as well as disturbance generated by amino acid substitutions. Although the remarkable heterogeneity of the mutational spectrum in Pompe disease was already known, our data demonstrate and confirm the power of molecular and functional analysis in predicting the natural course of Pompe disease. © 2008 Wiley‐Liss, Inc.
Dioxin and the Sex Ratio

The question of how exposure to dioxins might affect only males and why it affects preferentially male embryos in the Seveso data presented by Mocarelli et al. remains intriguing. This enigma can be explained by the ovopathy concept, which addresses the determination of both the sex and the condition of the progeny. Antiandrogenic properties of dioxin alter the sperm-transit time and mating behavior, which provoke delay of fertilization of the oocyte (postovulatory overripeness of the oocyte). Antiestrogenic properties of dioxin during mid-cycle compromise both mucus liquefaction and maturation of the oocyte (preovulatory overripeness ovopathy). A positive dose-response of male-biased pathologic conceptuses is often followed by a negative one due to "vanishing male conceptuses. " This dose-response fallacy is present in animal experiments and explains many otherwise unexplained phenomena related to dioxin contamination and other high-risk conceptions.
Risk Factors Associated with Progression to Blindness from Primary Open-Angle Glaucoma in an African-American Population

ABSTRACT Purpose: To determine the risk factors associated with progression to blindness from primary open-angle glaucoma (POAG) in an African-American population. Methods: This study examined 2119 patients enrolled in the Primary Open-Angle African-American Glaucoma Genetics (POAAGG) study. A total of 59 eyes were identified as legally blind as a result of POAG (cases) and were age-and sex-matched to 59 non-blind eyes with glaucoma (controls). Chart reviews were performed to record known and suspected risk factors. Results: Cases were diagnosed with POAG at an earlier age than controls (p = 0.005). Of the 59 eyes of cases, 16 eyes (27.1%) presented with blindness at diagnosis. Cases had worse visual acuity (VA) at diagnosis (p < 0.0001), with VA worse than 20/40 conferring a 27 times higher risk of progression to blindness (p = 0.0005). Blind eyes also demonstrated more visual field defects (p = 0.01), higher pre-treatment intraocular pressure (IOP; p < 0.0001), and higher cup-to-disc ratio (p = 0.006) at diagnosis. IOP was less controlled in cases, and those with IOP ≥21 mmHg at more than 20% of follow-up visits were 73 times more likely to become blind (p < 0.0001). Cases missed a greater number of appointments per year (p = 0.003) and had non-adherence issues noted in their charts more often than controls (p = 0.03). However, other compliance data did not significantly differ between groups. Conclusion: Access to care, initial VA worse than 20/40, and poor control of IOP were the major risk factors associated with blindness from POAG. Future studies should examine earlier, more effective approaches to glaucoma screening as well as the role of genetics in these significantly younger patients who progress to blindness.
Good Practices For Infection Prevention and Control at a Psychiatric Hospital in Brazil

This exploratory cross-sectional study aims to investigate good practice for preventing and controlling infections in a psychiatric hospital and for limiting potential exposure to biohazards for nursing professionals at this hospital located in the State of São Paulo, Brazil. The researchers directly and systematically observed 830 nursing procedures, 40.6% of which presented a moderate to high risk of biohazard exposure. Results indicate very low adherence to hand hygiene (1.2% before procedures, 2.9% after procedures), inappropriate use of gloves, and other instances of noncompliance to the standards of good practice for preventing and controlling infection, such as a lack of concurrent/terminal cleaning of dirty beds (132 instances) and careless manipulation of sharp devices (34 instances).
An overview of the influence of therapeutic ultrasound exposures on the vasculature: High intensity ultrasound and microbubble-mediated bioeffects

Abstract It is well established that the interaction of ultrasound with soft tissues can induce a wide range of bioeffects. One of the most important and complex of these interactions in the context of therapeutic ultrasound is with the vasculature. Potential vascular effects range from enhancing microvascular permeability to inducing vascular damage and vessel occlusion. While aspects of these effects are broadly understood, the development of improved approaches to exploit these effects and gain a more detailed mechanistic understanding is ongoing and largely anchored in preclinical research. Here a general overview of this established yet rapidly evolving topic is provided, with a particular emphasis on effects arising from high-intensity focused ultrasound and microbubble-mediated exposures.
Hierarchy-Based Link Prediction in Knowledge Graphs

Link prediction over a knowledge graph aims to predict the missing entity h or t for a triple (h,r,t). Existing knowledge graph embedding based predictive methods represent entities and relations in knowledge graphs as elements of a vector space, and employ the structural information for link prediction. However, knowledge graphs contain many hierarchical relations, which existing methods have pay little attention to. In this paper, we propose a hierarchy-constrained locally adaptive knowledge graph embedding based link prediction method, called hTransA, by integrating hierarchical structures into the predictive work. Experiments over two benchmark data sets demonstrate the superiority of hTransA.
Follow-up home visits with registered dietitians have a positive effect on the functional and nutritional status of geriatric medical patients after discharge: a randomized controlled trial

Objective: To assess the additional benefits of individualized nutritional counselling by a registered dietitian in geriatric patients’ home after discharge from hospital, in relation to risk of re-admissions, functional status, nutritional status, use of social services and mortality. Design: Twelve-week single-blind randomized controlled study. Setting and subjects: Geriatric medical patients (65+ years) at nutritional risk. Interventions: Participants were randomly allocated to receive a visit in their homes, either three individualized nutritional counselling by a registered dietitian complemented with three follow-up visits by general practitioners or three follow-up visits by general practitioners alone. Main measures: Primary outcome was risk of re-admissions. Secondary outcomes were functional status (hand grip strength, chair stand, mobility, disability and tiredness in daily activities, rehabilitation capacity), nutritional status (weight, BMI, energy and protein intake), need of social services (home care, home nursing, meals-on-wheels) and mortality. Results: One hundred and fifty-two patients were included; 132 (87%) completed the first and 124 (82%) the second data collection after 12 weeks. Ten per cent of the participants had three contacts with their general practitioner, while compliance with the dietetic intervention was almost 100%. Odds ratio for re-admission and mortality after 26 weeks was 1.62 (95% confidence interval (CI) 0.85 to 3.10) and 0.60 (95% CI 0.17 to 2.13). The intervention had a positive effect on functional status (i.e. mobility, P = 0.029), and nutritional status (i.e. weight, P = 0.035; energy intake, P < 0.001; protein intake, P = 0.001) and the use of meals-on wheels was reduced (P = 0.084). Conclusion: Follow-up home visits with registered dietitians have a positive effect on the functional and nutritional status of geriatric medical patients after discharge.
Suitability of Corncob Ash as a supplementary Cementitious Material

Cement is the most utilized construction material, and the second most consumed commodity in the world after water. Its demand has soared proportionately with the exponential rise in population in a bid to match the required development. The heavily energy-intensive processes that are involved in its production contribute to about 7 to 10 per cent (%) of the total global emissions, with potentially adverse environmental implications, and are also economically expensive. These processes, and generally those of the production of concrete consume heavily on natural resources such as sand, gravel, water, coal and crushed rock, mining of which mars the environment. It is however possible, that energy and cost efficiency can be achieved by reducing on the amount of clinker, and in its place utilising supplementary cementitious materials (SCMs) that require less process heating and emit fewer levels of carbon dioxide (CO2). This study investigated the ability of corncob ash (CCA) to be used as a SCM by testing for pozzolanic or hydraulic properties and performance in sulfate environments. Experiments were carried our by supplementing cement by weight in concrete mixes with CCA at 5%, 7.5%, 10%, 15%, 20%, 25% and 30% steps at the point of need. Results were compared with a control specimen, which was made with 100% cement. Durability was tested using the sulfate elongation test. The results showed impressive compressive strengths that were suitable for structural applications. It was concluded from the sulfate elongation test that CCA supplemented concrete could be used in aggressive environments with an advantage. The results showed good repeatability and highlight the potential of CCA as an effective pozzolan, which could enhance the sustainability and economic aspect of concrete, as well as improve its properties in both the wet and hardened states.
A General MIMO Framework for NOMA Downlink and Uplink Transmission Based on Signal Alignment

The application of multiple-input multiple-output (MIMO) techniques to nonorthogonal multiple access (NOMA) systems is important to enhance the performance gains of NOMA. In this paper, a novel MIMO-NOMA framework for downlink and uplink transmission is proposed by applying the concept of signal alignment. By using stochastic geometry, closed-form analytical results are developed to facilitate the performance evaluation of the proposed framework for randomly deployed users and interferers. The impact of different power allocation strategies, namely fixed power allocation and cognitive radio inspired power allocation, on the performance of MIMO-NOMA is also investigated. Computer simulation results are provided to demonstrate the performance of the proposed framework and the accuracy of the developed analytical results.
On the Origin of the Neutral Hydrogen Supershells: The Ionized Progenitors and the Limitations of the Multiple Supernovae Hypothesis

Here we address the question of whether the ionized shells associated with giant H II regions can be progenitors of the larger H I shell-like objects found in the Milky Way and other spiral and dwarf irregular galaxies. We use for our analysis a sample of 12 H II shells presented recently by Relaño et al. We calculate the evolutionary tracks that these shells would have if their expansion is driven by multiple supernovae explosions from the parental stellar clusters. We find, contrary to Relaño et al., that the evolutionary tracks of their sample H II shells are inconsistent with the observed parameters of the largest and most massive neutral hydrogen supershells. We conclude that H II shells found inside giant H II regions may represent the progenitors of small or intermediate H I shells; however, they cannot evolve into the largest H I objects unless, aside from the multiple supernovae explosions, an additional energy source contributes to their expansion.
Loss of methylation at GNAS exon A/B is associated with increased intrauterine growth.

CONTEXT GNAS is one of few genetic loci that undergo allelic-specific methylation resulting in the parent-specific expression of at least four different transcripts. Due to monoallelic expression, heterozygous GNAS mutations affecting either paternally or maternally derived transcripts cause different forms of pseudohypoparathyroidism (PHP), including autosomal-dominant PHP type Ib (AD-PHP1B) associated with loss of methylation (LOM) at exon A/B alone or sporadic PHP1B (sporPHP1B) associated with broad GNAS methylation changes. Similar to effects other imprinted genes have on early development, we recently observed severe intrauterine growth retardation in newborns, later diagnosed with pseudopseudohypoparathyroidism (PPHP) because of paternal GNAS loss-of-function mutations. OBJECTIVES This study aimed to determine whether GNAS methylation abnormalities affect intrauterine growth. PATIENTS AND METHODS Birth parameters were collected of patients who later developed sporPHP1B or AD-PHP1B, and of their healthy siblings. Comparisons were made to newborns affected by PPHP or PHP1A. RESULTS As newborns, AD-PHP1B patients were bigger than their healthy siblings and well above the reference average; increased sizes were particularly evident if the mothers were unaffected carriers of STX16 deletions. SporPHP1B newborns were slightly above average for weight and length, but their overgrowth was less pronounced than that of AD-PHP1B newborns from unaffected mothers. CONCLUSION LOM at GNAS exon A/B due to maternal STX16 deletions and the resulting biallelic A/B expression are associated with enhanced fetal growth. These findings are distinctly different from those of PPHP patients with paternal GNAS exons 2-13 mutations, whose birth parameters are almost 4.5 z-scores below those of AD-PHP1B patients born to healthy mothers.
Autoreactive B Cells Discriminate CpG-Rich and CpG-Poor DNA and This Response Is Modulated by IFN-α1

Autoreactive B cells are activated by DNA, chromatin, or chromatin-containing immune complexes (ICs) through a mechanism dependent on dual engagement of the BCR and TLR9. We examined the contribution of endogenous DNA sequence elements to this process. DNA sequence can determine both recognition by the BCR and by TLR9. DNA fragments containing CpG islands, a natural source of unmethylated CpG dinucleotides, promote the activation of DNA-reactive B cells derived from BCR transgenic mice as well as DNA-reactive B cells present in the normal repertoire. ICs containing these CpG island fragments are potent ligands for AM14 IgG2a-reactive B cells. In contrast, ICs containing total mammalian DNA, or DNA fragments lacking immunostimulatory motifs, fail to induce B cell proliferation, indicating that BCR crosslinking alone is insufficient to activate low-affinity autoreactive B cells. Importantly, priming B cells with IFN-α lowers the BCR activation threshold and relaxes the selectivity for CpG-containing DNA. Taken together, our findings underscore the importance of endogenous CpG-containing DNAs in the TLR9-dependent activation of autoreactive B cells and further identify an important mechanism through which IFN-α can contribute to the pathogenesis of systemic lupus erythematosus.
Use of the Italian translation of the Female Sexual Function Index (FSFI) in routine gynecological practice

Aims. To investigate domains of sexual function in healthy women attending a gynecological office for routine annual check-up using the Italian translation of the Female Sexual Function Index (FSFI) according to age, reproductive status and hormonal treatments; and to confirm the usefulness of the FSFI in detecting relevant clinical entities. Methods. Of 720 women (age range 18–65 years), 564 (78%) filled in a short anamnestic questionnaire and the FSFI assessing desire, arousal, lubrication, orgasm, satisfaction and pain. A semi-structured DSM-IV-TR clinical interview was administered to a convenience sample of women selected according to the quartile distribution of the median full scale FSFI score. Analysis of data was performed by frequency tables and non-parametric statistics. Results. The median full scale score of FSFI in our study population was 27.6 (lower quartile: 18.7, upper quartile: 30.9) and the percentage of women under the lower quartile of the distribution was 24.4%. Sexual function decreased progressively with age, being significantly lower after 30 years and after 60 years (χ2 = 52.6; p = 0.0001). Menopausal women had significantly lower median FSFI full scale score compared with fertile women and women who used oral contraception (OC) (p < 0.0001 for both), while users of hormone replacement therapy (HRT) displayed better overall sexual function than untreated postmenopausal women (p < 0.005). A positive diagnosis of female sexual dysfunction (FSD) was evident only in young women scoring under the lower quartile of the distribution (cut-off score: 23.4 for women not taking OC and 20.8 for OC users), while older women were dysfunctional also above the lower quartile of the distribution (cut-off score: 14.1 for menopause, 18.5 for HRT) of the FSFI full scale score. Conclusions. The FSFI is a powerful screening tool for FSD, especially in young fertile women, and may be used effectively in routine gynecological practice.
Drosophila Bitter Taste(s)

Most animals possess taste receptors neurons detecting potentially noxious compounds. In humans, the ligands which activate these neurons define a sensory space called “bitter”. By extension, this term has been used in animals and insects to define molecules which induce aversive responses. In this review, based on our observations carried out in Drosophila, we examine how bitter compounds are detected and if bitter-sensitive neurons respond only to molecules bitter to humans. Like most animals, flies detect bitter chemicals through a specific population of taste neurons, distinct from those responding to sugars or to other modalities. Activating bitter-sensitive taste neurons induces aversive reactions and inhibits feeding. Bitter molecules also contribute to the suppression of sugar-neuron responses and can lead to a complete inhibition of the responses to sugar at the periphery. Since some bitter molecules activate bitter-sensitive neurons and some inhibit sugar detection, bitter molecules are represented by two sensory spaces which are only partially congruent. In addition to molecules which impact feeding, we recently discovered that the activation of bitter-sensitive neurons also induces grooming. Bitter-sensitive neurons of the wings and of the legs can sense chemicals from the gram negative bacteria, Escherichia coli, thus adding another biological function to these receptors. Bitter-sensitive neurons of the proboscis also respond to the inhibitory pheromone, 7-tricosene. Activating these neurons by bitter molecules in the context of sexual encounter inhibits courting and sexual reproduction, while activating these neurons with 7-tricosene in a feeding context will inhibit feeding. The picture that emerges from these observations is that the taste system is composed of detectors which monitor different “categories” of ligands, which facilitate or inhibit behaviors depending on the context (feeding, sexual reproduction, hygienic behavior), thus considerably extending the initial definition of “bitter” tasting.
Impact of supplementary royal jelly on in vitro maturation of sheep oocytes: genes involved in apoptosis and embryonic development

Abstract Optimizing culture conditions lead to the improvement of oocyte developmental competence and additives with anti-oxidative activity in culture media improved embryonic development. Royal jelly (RJ) is a product from the cephalic glands of nurse bees that has considerable health effects. The aim of this study was to investigate the effect of different concentrations of RJ on the maturation, cleavage, and blastocyst rates and gene expression in the oocyte and cumulus cells during in vitro maturation (IVM) of sheep oocyte. IVM of oocyte was performed in the presence of control (RJ0), 2.5 (RJ2.5), 5 (RJ5), 10 (RJ10), 20 (RJ20), and 40 (RJ40) mg/mL of RJ. Following the maturation period, parthenogenetic activation was carried out in two treatment groups (RJ0 and RJ10) and embryonic development was examined three and eight days thereafter. Moreover, the relative expression of BCL2 and BAX in oocyte as well as BCL2, BAX, HAS2, PTGS2, and STAR in cumulus cells were assessed. The results indicated that the addition of 10 mg/mL of RJ (90 ± 4.51%) to the maturation medium linearly increased the oocyte maturation rate compared to the control group (57 ± 2.42%), then it remained constant to the RJ40 (93 ± 3.10%) group. The higher RJ concentrations were associated with increased (p < 0.01) cleavage (53.3 ± 1.55% to 82.3 ± 2.82%) and blastocyst rate (15.5 ± 1.16% to 33.8 ± 3.09%) from the RJ0 to the RJ10 group. The relative mRNA expression of BCL2 and BAX in the oocyte was higher at RJ10. In cumulus cells, the expression of BCL2 was not affected, but that of BAX decreased, and expression of HAS2, PTGS2, and STAR were increased following the addition of RJ to the maturation media. In conclusion, the addition of 10 mg/mL of RJ to maturation medium improved blastocyst formation and decreased the apoptotic incidence in sheep cumulus cells and the oocyte during the in vitro development.