Miracle-dz/UniFAR-FTUs huggingface dataset:
Cognitive, neuroanatomical and neuroendocrine effects of long-term rotating shift work in a nursing sample

Sleep disruption, like that experienced by long-term rotating shift workers, is a physiological stressor which causes a variety of adverse physical, psychological and cognitive symptoms. Some cognitive symptoms are thought to be mediated by the direct effect of stress hormones on the hippocampus. Regardless of its source, stress provokes endocrine responses in the body that affect the hypothalamic-pituitary-adrenal (HPA) axis. Whereas acute activation of the HPA axis adaptively activates the body’s stress response by increasing cortisol production, prolonged or repeated activation is detrimental to health due to dysregulation of the HPA axis. Cortisol affects the hippocampus, which has a high concentration of glucocorticoid receptors and plays a prominent role in the down-regulation of the HPA axis. Overstimulation of glucocortioid receptors can cause hippocampal atrophy and related cognitive deficits. Research has found that air crew with inadequate recovery time between outbound, transmeridian long-haul flights showed performance decrements on cognitive tasks, reduced hippocampal volumes and increased cortisol levels. The current study aimed to investigate whether work-related sleep disruption caused similar effects among rotating shift-workers from outside the flight industry. Twelve long-term female rotating shift-workers (nurses) and 17 day working female control participants (nurses and others) participated in the study. Analyses of the sleep health, cognitive (memory, attention, visual-spatial skills), endocrine (salivary cortisol), magnetic resonance imaging (MRI) and spectroscopy (MRS) data of these participants showed few differences between groups in cognitive performance, volumetric MRI or MRS. Shift workers reported less sleep over a fortnight, higher levels of fatigue and lower levels of vigor compared to controls. Cortisol rhythm changes including earlier morning rise and peak attenuation were apparent in shift workers. The results are interpreted in terms of age differences between the groups and the existence of a 'healthy worker' effect in the shift workers.
Family violence, negative outcomes and female delinquency : an empirical investigation using a Saudi Arabian sample

While there is extensive research on family violence and delinquency in Western literature, this topic is notably rare in Arab literature, including that from Saudi Arabia. Furthermore, although the association between being exposed to family violence and committing delinquent acts has been established in several Western studies, less is known about this association in the Saudi setting. This study sought to explore the extent and nature of these two phenomena in Saudi Arabia. It also aimed to examine the risk and protective factors that might be associated with the likelihood that girls who had experienced or witnessed family violence would then go on to commit delinquent acts. Given the dearth of literature on the association between family violence and female delinquency in Saudi Arabia, an exploratory study design was considered as the most appropriate method for this research. Data were collected through a self-completed questionnaire. The study sample consisted of 12- to 18-year-old female students (n=422) from intermediate and secondary schools in the Makkah area (western Saudi Arabia). Both family violence and female delinquency were found to have a relatively high prevalence. Physical abuse was shown to be the most common form of family violence, and brothers were the most common perpetrators of physical and sexual abuse. These results, which contrasted with a number of Western and other Saudi studies, point to sibling abuse as an area that is in need of further investigation. The current study indicated that violent offences, reported by 35 percent of the participants, were the most prevalent type of delinquency. This was unanticipated, given the highly conservative nature of Saudi society. The current study demonstrated significant associations between risk factors and the likelihood of female delinquency. In addition, the research suggests that if protective factors are absent or weak, then the likelihood that a young female will commit an antisocial behaviour offence is higher. Females exposed to family violence were found to be less likely to commit violent offences. The findings of the present study suggest that the problems of family violence and female delinquency are relatively common. More research is needed to assess the extent and nature of these problems at the national level. Moreover, the government should implement new policies and practices to address these problems head-on.
Brain Networks Underlying Episodic Memory Retrieval This Review Comes from a Themed Issue on Macrocircuits Memory Signals within the Mtl

The importance of the medial temporal lobe to episodic memory has been recognized for decades. Recent human fMRI findings have begun to delineate the functional roles of different MTL regions, most notably the hippocampus, for the retrieval of episodic memories. Importantly, these studies have also identified a network of cortical regions — each interconnected with the MTL — that are also consistently engaged during successful episodic retrieval. Along with the MTL these regions appear to constitute a content-independent network that acts in concert with cortical regions representing the contents of retrieval to support consciously accessible representations of prior experiences. Introduction Episodic memory — consciously accessible memory for unique events — allows us to represent past experiences and to flexibly employ these representations in service of current and future goals [1]. The present review focuses on recent human fMRI findings relevant to the functional neuroanatomy of successful episodic memory retrieval. The majority of the reviewed studies took as their starting point a 'dual-process' model of memory [2,3]. These models posit that a retrieval cue (such as a recognition memory test item) can elicit two qualitatively distinct kinds of mnemonic information: a multi-dimensional recollection signal that provides information about qualitative aspects of a prior event, including its context, and a scalar familiarity signal that can support simple judgments of prior occurrence. From this perspective, identifying the neural bases of episodic retrieval requires experimental designs that permit recollection-driven and familiarity-driven memory to be dissociated (Box 1). Current evidence suggests that the distinction between recollection and familiarity holds both within the MTL and at the level of the cerebral cortex, where a network of regions that appears to be preferentially engaged during successful recollection can be identified. The MTL — the hippocampus and surrounding peri-rhinal, entorhinal and parahippocampal cortices — has long been recognized as a key brain area supporting episodic memory. Reminiscent of electrophysiological findings in primates [4], fMRI studies have reported that perirhinal activity covaries inversely with the familiarity of recognition memory test items (e.g., [5]). These fMRI results are consistent with evidence from animal lesion studies [6] and a human single-case study [7] that suggest a pre-eminent role for perirhinal cortex in familiarity-based recognition. Perirhinal cortex is not, however, the only MTL region to demonstrate activity reductions for familiar recognition memory items, with several studies reporting similar findings for the hippocampus, in some cases seemingly in the same hippocampal regions that …
Progress in HIV-1 Integrase Inhibitors: A Review of their Chemical Structure Diversity

HIV-1 integrase (IN) enzyme, one of the three main enzymes of HIV-1, catalyzed the insertion of the viral DNA into the genome of host cells. Because of the lack of its homologue in human cells and its essential role in HIV-1 replication, IN inhibition represents an attractive therapeutic target for HIV-1 treatment. Since identification of IN as a promising therapeutic target, a major progress has been made, which has facilitated and led to the approval of three drugs. This review focused on the structural features of the most important IN inhibitors and categorized them structurally in 10 scaffolds. We also briefly discussed the structural and functional properties of HIV-1 IN and binding modes of IN inhibitors. The SAR analysis of the known IN inhibitors provides some useful clues to the possible future discovery of novel IN inhibitors.
Chronic degenerative radiculomyelopathy in the dog.

Chronic degenerative radiculomyelopathy (CDRM) is the name proposed for the chronic ataxic syndrome of older dogs previously reported as chronic ossifying pachymeningitis. The condition occurs in dogs of 6 years and older and affects large breeds. There is a slowly progressive ataxia and weakness of the hind limbs. Clinical testing demonstrates abnormalities in proprioception and touch and the nails of the affected leg(s) are usually worn. In the present series 70 % of cases had depression or absence of the patellar reflexes. Pain sensation, bladder control and the panniculus reflex are normal. There are degenerative lesions in the lumbar dorsal columns, fasciculus gracilis, lateral cortico spinal tract and around the ventro-median fissure. In many cases the dorsal nerve roots are also involved. The thoraco-lumbar grey matter and nucleus gracilis show marked astrocytic sclerosis. The distribution of the lesions suggests that this is a ‘dying back’ disease confined to the central nervous system.
Truncation of the COOH-terminal region of the paramyxovirus SV5 fusion protein leads to hemifusion but not complete fusion

The role of the simian virus 5 (SV5) fusion (F) protein 20 residue COOH- terminal region, thought to represent the cytoplasmic tail, in fusion activity was examined by constructing a series of COOH-terminal truncation mutants. When the altered F proteins were expressed in eukaryotic cells, by using the vaccinia virus-T7 transient expression system, all the F proteins exhibited similar intracellular transport properties and all were expressed abundantly on the cell surface. Quantitative and qualitative cell fusion assays indicated that all of the F protein COOH-terminal truncation mutants mediated lipid mixing with similar kinetics and efficiency as that of wild-type F protein. However, the cytoplasmic content mixing activity decreased in parallel with the extent of the deletion in the F protein COOH-terminal truncation mutants. These data indicate that it is possible to separate the presumptive early step in the fusion reaction, hemifusion, and the final stage of fusion, content mixing, and that the presence of the F protein COOH-terminal region is important for the final steps of fusion.
Response to letter regarding article,“cardiovascular events as a function of serum bilirubin levels in a large,statin-treated cohort”.

Mascitelli and Goldstein propose that lower levels of redox-active iron could explain some of the observed relationship between low serum bilirubin and the increased incidence of cardiovascular disease. Biliverdin/bilirubin, ferritin, and carbon monoxide are produced in equal quantities by heme-oxygenase–1, and the vascular effects of raised carbon monoxide may also contribute to the relationship between bilirubin and cardiovascular disease reported in our article. Disentangling the individual role of HO-1 products in cardiovascular disease will be difficult, although an independent effect of bilirubin is somewhat supported by Mendelian randomization designs using genetic variation strongly associated with bilirubin clearance. However, we should point out that these findings are not consistently replicated. We are not convinced that the results of our study can provide particularly strong support for the iron hypothesis, which remains an interesting area for research and debate.
Production, Fate and Pathogenicity of Plasma Microparticles in Murine Cerebral Malaria

In patients with cerebral malaria (CM), higher levels of cell-specific microparticles (MP) correlate with the presence of neurological symptoms. MP are submicron plasma membrane-derived vesicles that express antigens of their cell of origin and phosphatidylserine (PS) on their surface, facilitating their role in coagulation, inflammation and cell adhesion. In this study, the in vivo production, fate and pathogenicity of cell-specific MP during Plasmodium berghei infection of mice were evaluated. Using annexin V, a PS ligand, and flow cytometry, analysis of platelet-free plasma from infected mice with cerebral involvement showed a peak of MP levels at the time of the neurological onset. Phenotypic analyses showed that MP from infected mice were predominantly of platelet, endothelial and erythrocytic origins. To determine the in vivo fate of MP, we adoptively transferred fluorescently labelled MP from mice with CM into healthy or infected recipient mice. MP were quickly cleared following intravenous injection, but microscopic examination revealed arrested MP lining the endothelium of brain vessels of infected, but not healthy, recipient mice. To determine the pathogenicity of MP, we transferred MP from activated endothelial cells into healthy recipient mice and this induced CM-like brain and lung pathology. This study supports a pathogenic role for MP in the aggravation of the neurological lesion and suggests a causal relationship between MP and the development of CM.
HMM-Based Speech Enhancement Using Sub-Word Models and Noise Adaptation

This work proposes a method of speech enhancement that uses a network of HMMs to first decode noisy speech and to then synthesise a set of features that enables a clean speech signal to be reconstructed. Different choices of acoustic model (whole-word, monophone and triphone) and grammars (highly constrained to no constraints) are considered and the effects of introducing or relaxing acoustic and grammar constraints investigated. For robust operation in noisy conditions it is necessary for the HMMs to model noisy speech and consequently noise adaptation is investigated along with its effect on the reconstructed speech. Speech quality and intelligibility analysis find triphone models with no grammar, combined with noise adaptation, gives highest performance that outperforms conventional methods of enhancement at low signal-to-noise ratios.
Effects of stimulus and cell type on the expression of the -308 tumour necrosis factor promoter polymorphism.

The authors have previously demonstrated that the tumour necrosis factor (TNF) -308 G/A polymorphism affects the binding of transcription factors. In transient transfection assays in PMA stimulated U937 monocytes and Jurkat T cells, the A-containing TNF2 promoter has a 2-3-fold greater transcriptional activity than the TNF1 promoter in the presence of the TNF 3'UTR. In this study it was found that a difference in TNF1 and TNF2 promoter activities was only observed in U937 and Jurkat cells, and not in Raji (B cell line), HeLa (epithelial carcinoma cell line), HepG2 (hepatoma cell line) or THP-1 (monocyte), suggesting cell-type specific transcription factors or modifications may be involved in the formation of the -308 protein/DNA complex. Physiological stimulators, TNF and interferon gamma (IFN-gamma) did not cause differential promoter activity between TNF1 and TNF2, but LPS did with only the TNF2 promoter/3'UTR construct being significantly responsive to lipopolysaccharide (LPS) in U937 cells. In U937 cells, the -308 polymorphism affected transcription following differentiation by phorbol myristate acetate (PMA), retinoic acid, PMA plus LPS and PMA plus retinoic acid with an increase in nuclear factor binding to both TNF1 and TNF2 in the -323 to -285 region being observed. The greatest difference between TNF2 and TNF1 promoter activities (5-fold) was observed following PMA plus retinoic acid treatment of transfected U937 cells for 48h. During this time, U937 differentiated into cells with a macrophage-like morphology. An understanding of the cell type and stimuli specific requirements for differential expression of the -308 polymorphism may help elucidate the role the TNF -308 polymorphism plays in diseases where elevated TNF levels are thought to be important.
Me, Myself, and You: Friendships in Adolescence

The current study examines the role of similarity in music preferences in the formation and discontinuation of friendships over a one-year period. Questionnaire data were gathered from 283 Dutch same-sex mutual best friends (Mean age = 12.97) in two waves with a one-year interval. Results show consistent evidence for high similarity in specific music dimensions among friends at both waves. Moderate similarity was found in the overall patterning of preferences for music genres at both waves, even after controlling for similarity in social background. Specific music similarity in more non-mainstream music dimensions and overall music similarity at Wave 1 were related to selecting a new friend at Wave 2. However, similarity in music preferences was not related to the discontinuation of an existing friendship at Wave 2. Thus, results suggest that similarity in music preferences is related to friendship formation, and not to friendship discontinuation.
Mars North Polar Deposits: Stratigraphy, Age, and Geodynamical Response

The Shallow Radar (SHARAD) on the Mars Reconnaissance Orbiter has imaged the internal stratigraphy of the north polar layered deposits of Mars. Radar reflections within the deposits reveal a laterally continuous deposition of layers, which typically consist of four packets of finely spaced reflectors separated by homogeneous interpacket regions of nearly pure ice. The packet/interpacket structure can be explained by approximately million-year periodicities in Mars' obliquity or orbital eccentricity. The observed ∼100-meter maximum deflection of the underlying substrate in response to the ice load implies that the present-day thickness of an equilibrium elastic lithosphere is greater than 300 kilometers. Alternatively, the response to the load may be in a transient state controlled by mantle viscosity. Both scenarios probably require that Mars has a subchondritic abundance of heat-producing elements.
Calculation of influence of insulated bearings and insulated inner bearing seats on circulating bearing currents in machines of inverter-based drive systems

The high-frequency (HF) circulating bearing current that may occur in the machines of the inverter-based drive systems can be reduced or even completely suppressed by the use of the electrically insulated bearings or the insulated inner bearing seats. This paper proposes a method to calculate the influence of the thickness of the insulating coat on the obtained current reduction as a function of the several machine-design parameters. The parameters of the equivalent circuit are derived from an eddy-current model. The calculation considers the transient nature of the current flow. For the standard fan-cooled motors with up to 500-kW rated power, a minimum thickness of the insulating coat of 250 mum is required to reduce the circulating bearing currents to values where the apparent bearing-current density is less than 0.1 A/mm2, which is commonly taken as the threshold to the bearing failure
Relating whole-genome expression data with protein-protein interactions.

We investigate the relationship of protein-protein interactions with mRNA expression levels, by integrating a variety of data sources for yeast. We focus on known protein complexes that have clearly defined interactions between their subunits. We find that subunits of the same protein complex show significant coexpression, both in terms of similarities of absolute mRNA levels and expression profiles, e.g., we can often see subunits of a complex having correlated patterns of expression over a time course. We classify the yeast protein complexes as either permanent or transient, with permanent ones being maintained through most cellular conditions. We find that, generally, permanent complexes, such as the ribosome and proteasome, have a particularly strong relationship with expression, while transient ones do not. However, we note that several transient complexes, such as the RNA polymerase II holoenzyme and the replication complex, can be subdivided into smaller permanent ones, which do have a strong relationship to gene expression. We also investigated the interactions in aggregated, genome-wide data sets, such as the comprehensive yeast two-hybrid experiments, and found them to have only a weak relationship with gene expression, similar to that of transient complexes. (Further details on genecensus.org/expression/interactions and bioinfo.mbb.yale.edu/expression/interactions.)
The Anticonvulsant Enaminone E139 Attenuates Paclitaxel-Induced Neuropathic Pain in Rodents

The enaminone methyl 4-(4′-bromophenyl)aminocyclohex-3-en-6-methyl-2-oxo-1-oate (E139) has anticonvulsant activities. It has been reported to have a better safety profile than some anticonvulsant drugs. Since some anticonvulsant drugs are used in the management of neuropathic pain, we evaluated the effects of E139 in rodent models of acute pain and paclitaxel-induced neuropathic pain. The reaction latency to thermal stimuli (hot-plate test) of BALB/c mice was recorded before and after intraperitoneal treatment with paclitaxel (2 mg/kg, i.p. for 5 consecutive days), and after treatment with E139 (0.1–40 mg/kg), amitriptyline (10 mg/kg), and gabapentin (10 and 30 mg/kg). Mechanical allodynia in paclitaxel-treated Sprague Dawley (SD) rats was measured using a dynamic plantar aesthesiometer before and after treatment with E139 (10 and 20 mg/kg) or its vehicle for four consecutive days from day 7 after first administration of paclitaxel (16 mg/kg on two alternate days). Administration of E139 (10–40 mg/kg) produced antinociceptive activity against thermal nociception in naïve mice. Treatment with E139, amitriptyline, or gabapentin reduced paclitaxel-induced thermal hyperalgesia. E139 reduced paclitaxel-induced mechanical allodynia, with the effects lasting longer (24 h) after repetitive dosing. Our results indicate that E139 has antinociceptive activity and attenuates paclitaxel-induced neuropathic pain in rodents.
Epidemiology of gastric cancer in Japan

Despite its decreasing trend in Japan, gastric cancer remains an important public health problem. Although the age standardised rates of gastric cancer have been declining for decades, the absolute numbers are increasing because of the rapid aging of the population. A large proportion of Japanese gastric cancers are detected at an early stage, with a better overall survival rate. As with Western developed countries, a change in the social environment such as reduced salt use and increased fresh vegetable and fruit intake as well as improvement of food storage may play an important part in the decline. Differences in Helicobacter pylori infection rates between generations presumably have contributed to the generation related variation in the declining trends. It is expected that most gastric cancers in Japan may be preventable by lifestyle modification such as salt reduction and increased fruit and vegetable intake, together with avoidance of smoking and countermeasures against H pylori infection so that the level now evident in Western developed countries can be reached.