Miracle-dz/UniFAR-FTUs huggingface dataset:
BMPR1a and BMPR1b Signaling Exert Opposing Effects on Gliosis after Spinal Cord Injury

Astrogliosis following spinal cord injury (SCI) involves an early hypertrophic response that is beneficial and a subsequent formation of a dense scar. We investigated the role of bone morphogenetic protein (BMP) signaling in gliosis after SCI and find that BMPR1a and BMPR1b signaling exerts opposing effects on hypertrophy. Conditional ablation of BMPR1a from glial fibrillary acidic protein (GFAP)-expressing cells leads to defective astrocytic hypertrophy, increased infiltration by inflammatory cells, and reduced axon density. BMPR1b-null mice conversely develop “hyperactive” reactive astrocytes and consequently have smaller lesion volumes. The effects of ablation of either receptor are reversed in the double knock-out animals. These findings indicate that BMPR1a and BMPR1b exert directly opposing effects on the initial reactive astrocytic hypertrophy. Also, BMPR1b knock-out mice have an attenuated glial scar in the chronic stages following injury, suggesting that it has a greater role in glial scar progression. To elucidate the differing roles of the two receptors in astrocytes, we examined the effects of ablation of either receptor in serum-derived astrocytes in vitro. We find that the two receptors exert opposing effects on the posttranscriptional regulation of astrocytic microRNA-21. Further, overexpression of microRNA-21 in wild-type serum-derived astrocytes causes a dramatic reduction in cell size accompanied by reduction in GFAP levels. Hence, regulation of microRNA-21 by BMP signaling provides a novel mechanism for regulation of astrocytic size. Targeting specific BMPR subunits for therapeutic purposes may thus provide an approach for manipulating gliosis and enhancing functional outcomes after SCI.
Immunohistochemical demonstration of p63 in DMBA-induced hamster buccal pouch squamous cell carcinogenesis.

UNLABELLED Abnormalities in the p53 gene are regarded as the most consistent genetic abnormalities detected in head and neck squamous cell carcinogenesis. Two new members of the p53 gene family, p73 at the 1p36 region and p63 at the 3q27-29 region, have recently been identified. They share considerable sequence homology with p53 in the transactivation, DNA binding, and oligomerization domains, indicating possible involvement in carcinogenesis. To our knowledge, however, p63 expression in experimental oral carcinogenesis has not been studied. MATERIALS AND METHODS Immunohistochemical analysis of p63 protein expression was performed in 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch squamous cell carcinogenesis. Fifty outbred, young (6 weeks), male, Syrian golden hamsters (Mesocricatus auratus) were randomly divided into three experimental groups (each consisting of 10 3-, 9- and 15-week DMBA treated animals), and two control groups (with 10 animals in each). The pouches of the three experimental groups were painted bilaterally with a 0.5% DMBA solution three times a week. The treatment protocol for animals in one of the control groups was identical with only mineral oil applied, while the other control group remained untreated throughout the experiment. RESULTS In all of the untreated and mineral oil-treated pouch mucosa, nuclear positivity for p63 was mainly observed in the basal/parabasal cell layers. The p63 nuclear positivity extended from the basal/parabasal layers to the whole epithelial layers in the 3- and 9-week DMBA-treated pouch mucosa. Furthermore, the positive nuclear-stain cells were randomly distributed throughout the entire epithelial layers in the 3- and 9-week DMBA-treated pouch-mucosa specimens. In carcinomas from 15-week DMBA-treated pouch specimens, p63 staining was more uniform and homogeneous for the less-differentiated tumor areas. By contrast, p63 expression was noted mainly in the peripheral cells of tumor nests in the well-differentiated tumor areas. CONCLUSIONS The results of this study are consistent with those from previous analyses of p63 expression in human oral mucosa, suggesting that p63 may be associated with the regulation of epithelial differentiation and proliferation in DMBA-induced hamster buccal pouch squamous cell carcinogenesis. Further study is required to investigate which p63 isoform(s) is/are involved in hamster buccal pouch carcinogenesis.
Epidemiology of Tropical Neglected Diseases in Ecuador in the Last 20 Years

Background Tropical and zoonotic diseases are major problems in developing countries like Ecuador. Poorly designed houses, the high proportion of isolated indigenous population and under developed infrastructure represent a fertile environment for vectors to proliferate. Control campaigns in Ecuador over the years have had varying success, depending on the disease and vectors targeted. Aims In our study we analyse the current situation of some neglected diseases in Ecuador and the efficiency of the control campaigns (by measuring changes in numbers of cases reported) that the Ecuadorian government has been running to limit the spread of these infectious and parasitic diseases. Results Our study reveals that Brucellosis, Chagas Disease, Rabies and Onchocerciasis have been controlled, but small outbreaks are still detected in endemic areas. Leptospirosis and Echinococcosis have been increasing steadily in recent years in Ecuador since the first records. The same increase has been reported world-wide also. Better diagnosis has resulted in a higher number of cases being identified, particularly with regard to the linking of outdoor activities and contact with farm animals as contributing vectors. Improvements in diagnosis are due to regular professional training, implementation of automatized systems, establishing diagnosis protocols and the creation of an epidemiological vigilance network that acts as soon as a case is reported. Conclusion Control campaigns performed in Ecuador have been successful in recent years, although natural phenomena limit their efficiency. Leptospirosis and Echinococcosis infections remain a growing problem in Ecuador as it is worldwide.
Engineering Properties of Reactive Powder Concrete without Portland Cement

This paper reports the properties of a new type of high-performance composite material developed as an alternative construction material to reactive powder concrete (RPC) with a compressive strength over 200 MPa (29.0 ksi). This composite has been developed by the activation of slag and silica fume, without using portland cement. A comparative experimental study on the mechanical properties, microstructure, fresh-state properties, leaching of ions, bond to steel, and dimensional stability of RPC produced with portland cement (CRPC) and alkali-activated cement (ARPC) has been implemented. Test results show that ARPC has a very different microstructure with a great number of nano-sized pores compared to CRPC. Although these two RPC mixtures have similar compressive strength values, ARPC has significantly higher flexural performance, fracture energy, and bond to steel compared to CRPC. ARPC composites seem to be a good alternative to CRPC because of these superior properties and reduced environmental problems.
Using a Computer Game to Develop Advanced AI

Building software agents that can survive in the harsh environment of a popular computer game (Quake II) provides fresh insight into the study of artificial intelligence (AI). Our Quakebot uses Soar-an engine for making and executing decisions-as its underlying AI engine for controlling a single player. We chose Soar as our AI engine because our real research goal is to understand and develop general integrated intelligent agents.
Flutter in flow-limited collapsible tubes: a mechanism for generation of wheezes.

We studied flutter in collapsible tubes as a possible mechanism for the generation of respiratory wheezes. The pressure-flow relationships and the wall oscillations of thick-walled [wall thickness (h)-to-lumen radius (r) ratio 1:1.7 to 1.3] self-supporting latex and Silastic tubes mounted between rigid pipes were measured. A high-impedance vacuum pump was connected to the downstream end. Upstream and downstream valves were used to control corresponding resistances. We found loud honking sounds and tube wall oscillations that occurred only when the tubes were buckled and flow limiting, i.e., when the flow became constant and independent of downstream driving pressure. The overall range of oscillatory frequencies was 260-750 Hz for airflow, presenting as sharp peaks of power on the frequency spectrum. The oscillatory frequencies (f) were higher at higher fluid velocities (u) and with narrower distance between opposing flattened walls (2b), resulting from increasing downstream suction pressure and the transmural pressure becoming more negative. The effect of u and b on f for a latex tube (h-to-r ratio 1:1.7) were found to be f = 228 + 0.021 (u/b). These relationships were valid throughout the range of oscillations in this tube (283-720 Hz) and with flow rates of 12-64 l/min. The experimental data were compared with predictions of the fluid dynamic flutter theory and the vortex-induced wall vibrations mechanism. We conclude that viscid flutter in soft tubes is the more probable mechanism for the generation of oscillations in the soft tube model and is a possible mechanism for the generation of respiratory wheezes.
Effect of the root canal final rinse protocols on the debris and smear layer removal and on the push‐out strength of an epoxy‐based sealer

The aim of the present study was to evaluate the efficacy of QMiX, SmearClear, and 17% EDTA for the debris and smear layer removal from the root canal and its effects on the push‐out bond strength of an epoxy‐based sealer by scanning electron microscopy (SEM). Forty extracted human canines (n = 10) were assigned to the following final rinse protocols: G1‐distilled water (control), G2–17% EDTA, G3‐SmearClear, and G4‐QMiX. The specimens were submitted to a SEM analysis to evaluate the presence of debris and smear layer, respectively, in the apical or cervical segments. In sequence, forty extracted human maxillary canines with the root canals instrumented were divided into four groups (n = 10) similar to the SEM analysis study. After the filling with AH Plus, the roots were transversally sectioned to obtain dentinal slices. The specimens were submitted to a push‐out bond strength test using an electromechanical testing machine. The statistical analysis for the SEM and push‐out bond strength studies were performed using the Kruskal–Wallis and Dunn tests (α = 5%). There was no difference among the G2, G3, and G4 efficacy in removing the debris and smear layer (P > 0.05). The efficacy of these groups was superior to the control group. The push‐out bond strength values of G2, G3, and G4 were superior to the control group. The ability to remove the debris and smear layer by SmearClear and QMiX was as effective as the 17% EDTA. The final rinse with these solutions promoted similar push‐out bond strength values. Microsc. Res. Tech. 76:533–537, 2013. © 2013 Wiley Periodicals, Inc.
Genetic ACE I/D Polymorphism and Recurrence of Atrial Fibrillation After Catheter Ablation

Background—The angiotensin-converting enzyme (ACE) deletion allele, ACE D, is associated with increased cardiac ACE activity, cardiac fibrosis, and adverse outcomes in cardiovascular disease and has been linked with failure of antiatrial fibrillation (anti-AF) drug treatment. This study tested the hypothesis that the ACE gene insertion/deletion polymorphism associates with AF recurrence after catheter ablation. Methods and Results—In 238 consecutive patients (69% male; mean age, 58±11 years) undergoing catheter ablation of paroxysmal (59%) or persistent (41%) AF, the ACE insertion/deletion polymorphism was genotyped using polymerase chain reaction. After a blanking period of 3 months, AF recurrence (defined as any atrial arrhythmia lasting ≥30 s) was detected using serial 7-day Holter ECG recordings after 3, 6, and 12 months. AF recurrence was observed in 39% and was associated with persistent AF, longer history of AF, previous antiarrhythmic drug use, previous use of diuretics, increased left atrial diameter, increased left ventricular end-diastolic diameter, additional linear ablation lesions, and ACE DD polymorphism. In multivariable analysis, left atrial diameter (odds ratio, 1.111; 95% confidence interval, 1.040–1.187; P=0.002) and ACE DD genotype (odds ratio, 2.251; 95% confidence interval, 1.056–4.798; P=0.036) remained predictors for AF recurrence. Conclusions—Left atrial enlargement and the ACE DD polymorphism are predictors for AF recurrence after catheter ablation. The association between the ACE DD polymorphism and AF recidivism supports the use of genetic data for predicting response to AF therapies and highlights the role of fibrosis in AF development.
Carbon Nanotube Composite Sensor for Structural Health Monitoring of Bridge Structures

Bridges are important infrastructures of all nations and are required for transportation of goods as well as human. A catastrophic failure can result in loss of lives and enormous financial hardship to the nation. Although various kinds of sensors are now available to monitor the health of the structures due to corrosion, they do not provide permanent and long term measurements. This paper investigates the fabrication of Carbon Nanotube (CNT) based composite sensors for corrosion detection of structures. Multi-wall CNT (MWCNT)/Nafion composite sensors were fabricated to evaluate their electrical properties for corrosion detection. The test specimens were subjected to real life corrosion experimental tests and the results confirm that the electrical resistance of the sensor electrode was dramatically changed due to corrosion.
Rhetorical Structure Theory: looking back and moving ahead

Rhetorical Structure Theory has enjoyed continuous attention since its origins in the 1980s. It has been applied, compared to other approaches, and also criticized in a number of areas in discourse analysis, theoretical linguistics, psycholinguistics, and computational linguistics. In this article, we review some of the discussions about the theory itself, especially addressing issues of the reliability of analyses and psychological validity, together with a discussion of the nature of text relations. We also propose areas for further research. A follow-up article (Taboada and Mann, forthcoming) will discuss applications of the theory in various fields.
Parallel deficits in linear growth and mental development in low-income Mexican infants in the second year of life

Abstract Objective To explore anthropometric indicators and mental development in very-low-income children in the second year of life. Design Cross-sectional survey. Setting Low-income areas (income <20th percentile) in semi-urban Mexico (defined as towns or cities with 2500–50 000 inhabitants). Subjects Eight hundred and ninety-six children aged 12.5–23.5 months surveyed from September to December 2001. Methods Questionnaire survey and anthropometric survey of households. Multivariate regression models evaluated differences across age in anthropometry (height-for-age Z-score (HAZ) and weight-for-height Z-score) and cognitive function (Mental Development Index (MDI) of the Bayley Scales of Infant Development) while controlling for socio-economic and parental characteristics. Results There was a significant decline in HAZ and in age-adjusted MDI score across the second year of life. Although the children showed MDI scores close to the mean, normed US values at 13–14 months, the scores were significantly lower than expected in older children (P < 0.0001), even after controlling for socio-economic status and parental characteristics. At 13–14 months, only 3% of children received scores below 70 (less than minus two standard deviations), whereas by 19–20 months, almost 17% of children were performing below this level. No socio-economic or parental characteristics were significant predictors of HAZ or MDI. Conclusions Parallel deficits are evident in both height-for-age and cognitive functioning during the second year of life in low-income Mexican infants. The consistency of these growth and development findings further stresses the need for targeted interventions to reduce the vulnerability of low-income Mexican children very early in life.
Hue-preserving color image enhancement without gamut problem

The first step in many techniques for processing intensity and saturation in color images keeping hue unaltered is the transformation of the image data from RGB space to other color spaces such as LHS, HSI, YIQ, HSV, etc. Transforming from one space to another and processing in these spaces usually generate a gamut problem, i.e., the values of the variables may not be in their respective intervals. We study enhancement techniques for color images theoretically in a generalized setup. A principle is suggested to make the transformations gamut-problem free. Using the same principle, a class of hue-preserving, contrast-enhancing transformations is proposed; they generalize existing grey scale contrast intensification techniques to color images. These transformations are also seen to bypass the above mentioned color coordinate transformations for image enhancement. The developed principle is used to generalize the histogram equalization scheme for grey scale images to color images.
Reliability of the Strengths and Difficulties Questionnaire in Japanese Preschool Children Aged 4-6 Years.

Background: The Strengths and Difficulties Questionnaire (SDQ) has been widely used as a brief behavioral screening. The aim of this study was to examine the internal consistency and test-retest reliability of the 3- to 4-year-old version of the SDQ (SDQ 3-4) in Japanese preschool children.Methods: The SDQ 3-4 was administered to 754 parents who had 4- to 6-year-old children attending kindergartens or childcare centers in Wako City, Japan, at 2 different times (Time 1 and Time 2) over a 2-week interval between June and July 2012. Cronbach's α and correlation coefficients were used to examine internal consistency and test-retest reliability, respectively.Results: Of 393 parents who returned their responses at Time 1 (response rate 52.1%), 383 were used for analysis after excluding 10 responses with missing data. Their children's mean age was 4.7 (standard deviation 0.7) years. The internal consistency (Cronbach's α) was good for the total difficulties score (0.74) and the prosocial behavior scale (0.70). However, it was slightly worse for the emotional symptoms, conduct problems, and hyperactivity scales (0.61-0.66) and poor for the peer problems scale (0.45). Of the 383 included respondents at Time 1, 211 parents returned their responses at Time 2 (response rate: 55.1%). Test-retest reliability (correlation coefficients) was good (0.73-0.82), except for the peer problems scale (0.58). Conclusions: The results support the reliability of the SDQ 3-4 being satisfactory for the total difficulties score and prosocial behavior scale and being acceptable for the emotional symptoms, conduct problems, and hyperactivity scales in Japanese preschool children aged 4-6 years.
Distinct H‐2 complex control of mortality, and immune responses to tuberculosis infection in virgin and BCG‐vaccinated mice

We have studied the impact of distinct haplotypes and of difierent alleles at specific H‐2 loci on: (i) the susceptibility to lethal form of experimental tuberculosis; (ii) the level of DTH to mycobacterial antigens: (iii) the efficacy of vaccination with bacille Calmette‐Guerin (BCG); and (iv) the IgG production and T cell proliferative response to H37Rv antigens. On the basis of median survival lime (MST) following primary inoculation with lethal dose of Mycobacterium tuberculosis, susceptibility to infection associated with I‐Ab and Db alleles. host resistance associated with I‐Ak and Dd alleles. Mice bearing a disease‐resistant phenotype also developed a vigorous DTH response. Vaccination with BCG before H37Rv infection significantly prolonged the survival time of both resistant and susceptible animals, except in B10.M (H‐2f) mice. The latter exhibited intermediate resistance to infection before but slight decrease in the MST following a high‐dose BCG vaccination. Distinct H‐2 regulation of susceptibility to lethal infection and of BCG vaccination efficacy was confirmed in another relatively resistant H‐2f‐bearing strain A.CA, in which mortality occurred more rapidly in vaccinated compared with primarily infected animals. The expression of the H‐2f haplotype was associated with a low DTH response to tuberculin following vaccination and subsequent lethal infection. The lack of BCG protection against Myco. tuberculosis challenge in B10.M mice associated with the high litre of specific IgG. In addition, these mice exhibited a unique ability to respond to 65‐kD antigen by both IgG synthesis and T cell proliferation.
Psychomotor epilepsy and psychosis. II PHYSICAL ASPECTS

In a controlled investigation the clinical findings in 96 patients with paranoid/hallucinatory psychosis and partial epileptic seizures with complex symptoms, were compared with the findings in 96 control patients with the same type of epilepsy without psychosis of median 24 years' duration. The median age at onset of psychosis was 34 years, after epilepsy of median 21 years' duration. The seizure frequency of complex, partial seizures was significantly lower in the psychotic group, while the frequency of generalized seizures did not differ. A significant preponderance in the psychotic group of left‐handed patients, etiological factors and neurological signs reflecting organic damage, and seizures of automatic behaviour indicates that epileptic psychoses are caused by structural lesions affecting the deep parts of the temporal lobe.
Evolutionary Design of Intelligent Controller for a Cement Mill Process

The Knowledge Base of a Fuzzy Logic Controller (FLC) encapsulates expert knowledge and consists of the Data Base (membership functions) and Rule-Base of the controller. Optimization of these Knowledge Base components is critical to the performance of the controller and has traditionally been achieved through a process of trial and error. Such an approach is convenient for FLCs having low numbers of input variables however for greater number of inputs, more formal methods of Knowledge Base optimization are required. Genetic Algorithms (GAs) provide such a method to optimize the FLC parameters. An intelligent multi input multi output (MIMO) control for the cement milling circuit is presented. The FLC is optimized by GA for varying nonlinearity in the plant. The proposed control algorithm was tested on the cement mill simulation model within MATLABTM SimulinkTM environment. Parameters of the simulation model were set up based on the actual cement mill characteristics. The performances of the proposed control technique are compared with various control technique. The results of the control study indicate that the proposed algorithm can prevent the mill from plugging and control the cement mill circuit effectively compared to the other control technique.