Miracle-dz/UniFAR-FTUs huggingface dataset:
Specific Behavior of Intracellular Streptococcus pyogenes That Has Undergone Autophagic Degradation Is Associated with Bacterial Streptolysin O and Host Small G Proteins Rab5 and Rab7*

Streptococcus pyogenes (group A streptococcus (GAS)) is a pathogen that invades non-phagocytic host cells, and causes a variety of acute infections such as pharyngitis. Our group previously reported that intracellular GAS is effectively degraded by the host-cell autophagic machinery, and that a cholesterol-dependent cytolysin, streptolysin O (SLO), is associated with bacterial escape from endosomes in epithelial cells. However, the details of both the intracellular behavior of GAS and the process leading to its autophagic degradation remain unknown. In this study, we found that two host small G proteins, Rab5 and Rab7, were associated with the pathway of autophagosome formation and the fate of intracellular GAS. Rab5 was involved in bacterial invasion and endosome fusion. Rab7 was clearly multifunctional, with roles in bacterial invasion, endosome maturation, and autophagosome formation. In addition, this study showed that the bacterial cytolysin SLO supported the escape of GAS into the cytoplasm from endosomes, and surprisingly, a SLO-deficient mutant of GAS was viable longer than the wild-type strain although it failed to escape the endosomes. This intracellular behavior of GAS is unique and distinct from that of other types of bacterial invaders. Our results provide a new picture of GAS infection and host-cell responses in epithelial cells.
Non-Linear Performance of Slabs in Coupled Shear Wall Structures

The non-linear coupling action of reinforced concrete slabs in shear wall structures is investigated by the finite element method and the results are validated by small scale model tests using micro-concrete. Flexural stiffness and effective width of the coupling slabs are found to be influenced by the geometric and non-linear material parameters of the structures. Design curves for the determination of flexural stiffness and effective width are presented as functions of geometric parameters in the pre-cracking, cracking and post-yielding stages of non-linear material response. Comparative study of numerical and experimental results suggested that the design curves are reliable and can be used in the analysis and design of shear wall structure in practical situations.
When does an abbreviation become a word? and related questions

An experiment is reported in which subjects previously naive to text editing learned to use a set of editing commands. Some subjects used abbreviations from the beginning. Others began by using full command names, then switched to the (optional) use of abbreviations, either of their own devising or of our selection. We found significant differences in the number and nature of the errors produced by subjects in the different conditions. People who created their own abbreviations did most poorly, and did not appear to learn from this experience. Those who used abbreviations from the start were more likely to fall into error through misrecalling the referent names. The results suggest aspects of the underlying cognitive representations, with implications for the design of software interfaces.
Pseudocyesis: A complication of antipsychotic-induced increased prolactin levels and weight gain

Pseudocyesis or phantom pregnancy is characterized by a false belief in a non-pregnant female that she is pregnant and this belief is usually associated with bodily signs of pregnancy. In some of the patients, this belief is held with delusional conviction. In this case report, we present the case of a female patient who presented with delusional belief of being pregnant, which was associated with antipsychotic-associated increase in prolactin levels and metabolic syndrome.
Inflammatory triggers of acute rejection of organ allografts

Solid organ transplantation is a vital therapy for end stage diseases. Decades of research have established that components of the adaptive immune system are critical for transplant rejection, but the role of the innate immune system in organ transplantation is just emerging. Accumulating evidence indicates that the innate immune system is activated at the time of organ implantation by the release of endogenous inflammatory triggers. This review discusses the nature of these triggers in organ transplantation and also potential mediators that may enhance inflammation resolution after organ implantation.
Welding of thick austenitic stainless steel using Nd:yttrium-aluminum-garnet laser with filler wire and hybrid process

Autogenous laser welding has shown many advantages over traditional welding methods in numerous applications. However, there could be even more applications, but due to the power levels of present high power lasers, depth of penetration is limited. One way to overcome this limitation is to use multipass laser welding, in which a narrow gap and a filler wire are applied. By this process thick sections can be welded with a smaller heat input and therefore with smaller distortions, and the process seems to be very effective compared to “traditional” welding methods. Another way to increase penetration and fill the groove is by using the so-called hybrid process, in which laser and GMAW are combined. In this study thick section austenitic stainless steel is welded using a multipass technique with filler wire, and also by utilizing a hybrid process. For narrow gap conditions, groove angles of 8°, 10°, and 12°, are used with a partially grooved V joint. Parameters (e.g, filler wire feeding, placement of wire, a.
Reversal of the physiological effects of monocular deprivation in kittens: further evidence for a sensitive period

1. It was confirmed that suturing the lids of one eye (monocular deprivation), until only 5 weeks of age, leaves virtually every neurone in the kitten's visual cortex entirely dominated by the other eye. On the other hand, deprivation of both eyes causes no change in the normal ocular dominance of cortical neurones, most cells being clearly binocularly driven.
Antioxidant Activities, Total Phenolics and Flavonoids Content in Two Varieties of Malaysia Young Ginger (Zingiber officinale Roscoe)

Ginger (Zingiber officinale Roscoe) is a well known and widely used herb, especially in Asia, which contains several interesting bioactive constituents and possesses health promoting properties. In this study, the antioxidant activities of methanol extracts from the leaves, stems and rhizomes of two Zingiber officinale varieties (Halia Bentong and Halia Bara) were assessed in an effort to compare and validate the medicinal potential of the subterranean part of the young ginger. The antioxidant activity and phenolic contents of the leaves as determined by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the total amounts of phenolics and flavonoids were higher than those of the rhizomes and stems. On the other hand, the ferric reducing/antioxidant potential (FRAP) activity of the rhizomes was higher than that of the leaves. At low concentration the values of the leaves’ inhibition activity in both varieties were significantly higher than or comparable to those of the young rhizomes. Halia Bara had higher antioxidant activities as well as total contents of phenolic and flavonoid in comparison with Halia Bentong. This study validated the medicinal potential of the leaves and young rhizome of Zingiber officinale (Halia Bara) and the positive relationship between total phenolics content and antioxidant activities in Zingiber officinale.
Learning to Relate Images.

A fundamental operation in many vision tasks, including motion understanding, stereopsis, visual odometry, or invariant recognition, is establishing correspondences between images or between images and data from other modalities. Recently, there has been an increasing interest in learning to infer correspondences from data using relational, spatio-temporal, and bi-linear variants of deep learning methods. These methods use multiplicative interactions between pixels or between features to represent correlation patterns across multiple images. In this paper we review the recent work on relational feature learning, and we provide an analysis of the role that multiplicative interactions play in learning to encode relations. We also discuss how square-pooling and complex cell models can be viewed as a way to represent multiplicative interactions and thereby as a way to encode relations.
Motion Correction Strategies for Integrated PET/MR

Integrated whole-body PET/MR facilitates the implementation of a broad variety of respiratory motion correction strategies, taking advantage of the strengths of both modalities. The goal of this study was the quantitative evaluation with clinical data of different MR- and PET-data–based motion correction strategies for integrated PET/MR. Methods: The PET and MR data of 20 patients were simultaneously acquired for 10 min on an integrated PET/MR system after administration of 18F-FDG or 68Ga-DOTANOC. Respiratory traces recorded with a bellows were compared against MR self-gating signals and signals extracted from PET raw data with the sensitivity method, by applying principal component analysis (PCA) or Laplacian eigenmaps and by using a novel variation combining the former and either of the latter two. Gated sinograms and MR images were generated accordingly, followed by image registration to derive MR motion models. Corrected PET images were reconstructed by incorporating this information into the reconstruction. An optical flow algorithm was applied for PET-based motion correction. Gating and motion correction were evaluated by quantitative analysis of apparent tracer uptake, lesion volume, displacement, contrast, and signal-to-noise ratio. Results: The correlation between bellows- and MR-based signals was 0.63 ± 0.19, and that between MR and the sensitivity method was 0.52 ± 0.26. Depending on the PET raw-data compression, the average correlation between MR and PCA ranged from 0.25 ± 0.30 to 0.58 ± 0.33, and the range was 0.25 ± 0.30 to 0.42 ± 0.34 if Laplacian eigenmaps were applied. By combining the sensitivity method and PCA or Laplacian eigenmaps, the maximum average correlation to MR could be increased to 0.74 ± 0.21 and 0.70 ± 0.19, respectively. The selection of the best PET-based signal for each patient yielded an average correlation of 0.80 ± 0.13 with MR. Using the best PET-based respiratory signal for gating, mean tracer uptake increased by 17 ± 19% for gating, 13 ± 10% for MR-based motion correction, and 18 ± 15% for PET-based motion correction, compared with the static images. Lesion volumes were 76 ± 31%, 83 ± 18%, and 74 ± 22% of the sizes in the static images for gating, MR-based motion correction, and PET-based motion correction, respectively. Conclusion: Respiratory traces extracted from MR and PET data are comparable to those based on external sensors. The proposed PET-driven gating method improved respiratory signals and overall stability. Consistent results from MR- and PET-based correction methods enable more flexible PET/MR scan protocols while achieving higher PET image quality.
Intravoxel incoherent motion diffusion-weighted MR imaging of hepatocellular carcinoma: correlation with enhancement degree and histologic grade.

PURPOSE To compare the association of intravoxel incoherent motion (IVIM)-derived parameters and apparent diffusion coefficient (ADC) with the histologic grade of hepatocellular carcinoma (HCC) and evaluate the relationship between IVIM-derived parameters and arterial enhancement degree. MATERIALS AND METHODS This retrospective study was institutional review board-approved, and informed consent was waived. Forty patients with 42 surgically confirmed HCCs underwent diffusion-weighted magnetic resonance (MR) imaging with eight b values (0-800 sec/mm(2)). ADC, diffusion coefficient (D), pseudodiffusion coefficient, and perfusion fraction (f) were calculated. Two radiologists determined the enhancement degree in consensus, as well as the percentage of arterial enhancement of HCC. The relationship between the parameters and histologic grade, as well as arterial enhancement, was assessed by using the Spearman or Pearson correlation test. Receiver operating characteristic (ROC) analysis of discrimination between low-grade (grades 1 and 2) and high-grade (grades 3 and 4) HCC was performed for D and ADC values. RESULTS D and ADC values were both significantly correlated with histologic grade: r = -0.604 (P < .0001) and r = -0.448 (P = .002), respectively. D and ADC values were both significantly lower in high-grade HCC (D = [0.99 ± 0.13] × 10(-3)mm(2)/sec, ADC = [1.13 ± 0.14] × 10(-3)mm(2)/sec) than in low-grade HCC (D = [1.18 ± 0.16] × 10(-3)mm(2)/sec, ADC = [1.25 ± 0.17] × 10-(3)mm(2)/sec) (P < .0001 and P = .029, respectively). However, ROC analysis demonstrated a higher area under the ROC curve value for D than for ADC for differentiating high-grade HCC from low-grade HCC (0.838 vs 0.728; P = .026). The percentage of arterial enhancement was correlated with f (r = 0.621, P < .0001). CONCLUSION IVIM-derived D values of HCC showed significantly better diagnostic performance than ADC values in differentiating high-grade HCC from low-grade HCC, and significant correlation was observed between f and the percentage of arterial enhancement.
The growth of spinel into AI2O3

Abstract The growth of cobalt aluminate spinel into an a-alumina substrate has been studied using transmission electron microscopy by combining imaging and selected area electron diffraction. It was found that for a (0001) alumina substrate the (111) plane of the spinel is usually slightly inclined to the basal plane of the a-alumina. The phase transformation does not therefore take place simply by the motion of Shockley partial dislocations as had previously been proposed. Models for the structure of the phase boundary are discussed and a new model proposed based on the new experimental results.
Morphology of Films of SEBS Triblock Copolymers

Surface morphologies of the films of poly (styrene-6-(ethylene-cp-butene)-b-styrene) (SEBS) have been studied by using tapping-mode atomic force microscopy (TM-AFM). The films of block copolymer were prepared both by spin-coating on mica and by solvent-casti ng on different solution surfaces. For spin-coating samples, the effect of solution concentration, solvent, and annealing temperature are investigated. It is shown that changing the concentration of the solution makes no difference on the morphology of the film of the block copolymer. The microstructures are quite stable during thermal annealing; only the size of the domains changes toward the equilib- rium configuration. However, solvent annealing can notably change the microstructures. When different selective solvents are used for film spin-coating, different morphologies can be obtained and explained by the different solu- bility parameters of the solvents. As expected, significant different morphologies in the top and the bottom surfaces of the casting films were observed. The images of the top surfaces reveal cylinder microdomains, while those of the bottom surfaces were spherical morphologies. Keywords atomic force microscopy (AFM), morphology, block copolymer, spin-coating, solvent-casting
Effect of Ascorbic Acid and Potassium Chloride Supplementation on Performance and Some Physiological Parameters in Broiler Chicks Reared under Summer Condition

A total of 420, one day-old broiler chickens were randomly divided into 4 groups and kept under elevated summer temperature to investigate the effect of ascorbic acid and potassium chloride on the productive performance Live Body Weight (LBW), Body Weight Gain (BWG), Feed Consumption (FC), Feed Conversion Ratio (FCR), Mortality, Dressing Percentage (DP) and blood parameter (hemoglobin (Hb), Packet Cell Value (PCV), Red Blood Cell (RBCs), pH and serum glucose) and rectum temperature. Heat stress was associated with an increase in FCR and mortality and a decreased LBW, BWG and FC of birds. Ascorbic acid and potassium chloride supplementation during heat stress had a beneficial effect on LBW, BWG, FC, FCR, DP, mortality, pH, Serum Glucose and rectum temperature.
Function and Redundancy of the Chaplin Cell Surface Proteins in Aerial Hypha Formation, Rodlet Assembly, and Viability in Streptomyces coelicolor

ABSTRACT The chaplins are a family of eight secreted proteins that are critical for raising aerial hyphae in Streptomyces coelicolor. These eight chaplins can be separated into two main groups: the long chaplins (ChpA to -C) and the short chaplins (ChpD to -H). The short chaplins can be further subdivided on the basis of their abilities to form intramolecular disulfide bonds: ChpD, -F, -G, and -H contain two Cys residues, while ChpE has none. A “minimal chaplin strain” containing only chpC, chpE, and chpH was constructed and was found to raise a substantial aerial mycelium. This strain was used to examine the roles of specific chaplins. Within this strain, the Cys-containing ChpH was identified as the major polymerization unit contributing to aerial hypha formation and assembly of an intricate rodlet ultrastructure on the aerial surfaces, and the two Cys residues were determined to be critical for its function. ChpC augmented aerial hypha formation and rodlet assembly, likely by anchoring the short chaplins to the cell surface, while ChpE was essential for the viability of wild-type S. coelicolor. Interestingly, the lethal effects of a chpE null mutation could be suppressed by the loss of the other chaplins, the inactivation of the twin arginine translocation (Tat) secretion pathway, or the loss of the rodlins.
Regulation of Quorum Sensing by RpoS inPseudomonas aeruginosa

ABSTRACT The LasR-LasI and RhlR-RhlI quorum-sensing systems are global regulators of gene expression in the opportunistic pathogenPseudomonas aeruginosa. Previous studies suggest that the RhlR-RhlI system activates expression of rpoS. We constructed merodiploid strains of P. aeruginosa containing the native rpoS gene and an rpoS-lacZ fusion. Studies of lacZ transcription in these strains indicated that rpoS was not regulated by RhlR-RhlI. We also generated an rpoS null mutant. This rpoS mutant showed elevated levels of rhlI (but not rhlR) transcription, elevated levels of the RhlI-generated acylhomoserine lactone quorum-sensing signal, and elevated levels of RhlR-RhlI-regulated gene transcription. These findings indicate that there is a relationship between RpoS and quorum sensing, but rather than the RhlR-RhlI system influencing the expression ofrpoS, it appears that RpoS regulates rhlI.
Intratumoral and peritumoral expression of CD68 and CD206 in hepatocellular carcinoma and their prognostic value

The aims of the present study were to determine whether the changes in density and location of CD68-positive and CD206-positive macrophages contribute to progression of hepatocellular carcinoma (HCC) and to evaluate prognostic values of these cells in post-surgical patients. A retrospective study involving 268 HCC patients was conducted. CD68-positive and CD206-positive macrophage infiltration in HCC tissues and adjacent tissues was examined by immunohistochemistry (IHC) and the relationship between the clinicopathological features and prognosis was analyzed. Receiver operating characteristics (ROC) curve was used to calculate diagnostic accuracy. There was an increase in CD68-positive and CD206-positive macrophage infiltration in adjacent tumor tissues compared with tumor tissues. ROC curve identified their optimal diagnostic cut-off values. The survival analysis showed that increased CD68 expression in adjacent tissues conferred superior overall survival (OS) and disease-free survival (DFS), while increase of CD206 in tumor yielded inferior OS and DFS. Cox regression analysis suggested both CD68-positive macrophages in adjacent area and intratumor CD206-positive macrophages as independent prognostic biomarkers for post-surgical HCC patients. Finally, a combination of CD68/CD206 and HBV-positive further improved prognostic stratification, especially in DFS. These results provide the first evidence for region- and subset-dependent involvement of CD68 and CD206 cells in HCC progression. A combination of CD68/CD206 density and HBV-positivity improves further predictive value for post-operative recurrence of HCC. Quantification of CD68/CD206 macrophages and their distribution can be exploited for better postsurgical management of HCC patients. These findings provide a basis for developing novel treatment strategies aimed at re-educating macrophages in tumor microenvironment.
Heart rate measurement in neonatal patients using a webcamera

At present there is a clear need for non-contact monitoring of the physiological signs of the patients. The system proposed in this paper aims to measure the heart rate of neonatal infants without any direct contact with the patient. The solution proposed is based on the use of standard, low-cost and commercially available digital webcamera by which it has been possible to observe defined portions of the patient face; the sequence of such images has then been used, by a specifically developed algorithm (based on Indipendent Component Analysis), to extract the heart rate of the patients. Data collected on 7 patients demonstrate the feasibility of the measurement method proposed. Data acquired on the same patients with standard electrocardiography (ECG) has been used for comparison. Bland-Altman analysis of data show close correlation of the heart rates measured with the two approaches (correlation coefficient of 0.94) with an uncertainty of 4.5 bpm (k=1). This technique has a valuable interest for the use in clinical environment as non-contact, easily deployable and economic monitoring system, but it also shows an interesting potential for remote, home health monitoring.
Differential Wnt Pathway Gene Expression and E-Cadherin Truncation in Sporadic Colorectal Cancers with and without Microsatellite Instability

Purpose: Alterations in the Wnt pathway play a major role in colorectal cancer with high (MSI-H) or low microsatellite instability (MSS/MSI-L). However, the differential impact of the Wnt pathway components on these tumors is poorly understood. MMP-3 (stromelysin-1) promoter is a target of the mutator phenotype in sporadic colorectal cancer. Among MMP-3 targets, we investigated E-cadherin integrity status in both groups of tumors. Because beta-catenin is the main effector of the Wnt pathway, we have also investigated the differential cellular status of beta-catenin. Experimental Design: Expression profiles of 114 genes related to the Wnt pathway were analyzed by oligo microarrays in 48 tumors classified by their MSI status. In addition, we analyzed 48 sporadic colorectal cancers for E-cadherin integrity status. We performed investigation of beta-catenin and cyclin D1 by immunohistochemistry using tissue arrays containing 96 tumors. Results: Our data show that a group of genes that negatively regulate Wnt signaling are downregulated in MSS/MSI-L as compared with MSI-H colorectal tumors. E-cadherin truncation was significantly higher in MSS/MSI-L as compared with MSI-H tumors. Moreover, MSI-H tumors showed low or null beta-catenin nuclear presence, whereas the group of tumors classified as MSS or MSI-L displayed a high content of the nuclear beta-catenin location. Conclusions: Our results suggest that the differential expression of genes that negatively regulate the Wnt pathway, as well as the status of E-cadherin and beta-catenin in MSI-H or MSS/MSI-L colorectal tumors, shed some light on the different clinical behavior showed by the two groups.