Miracle-dz/UniFAR-FTUs huggingface dataset:
Four patterns of response to inhaled nitric oxide for persistent pulmonary hypertension of the newborn.

OBJECTIVE To determine the clinical role of inhaled nitric oxide (iNO) in the treatment of persistent pulmonary hypertension of the newborn (PPHN). STUDY DESIGN Prospective open observational clinical study. SETTING A regional cardiac and pediatric intensive care unit. METHODS Twenty-five consecutive near-term neonates (> 35 weeks gestation) with severe PPHN (oxygenation index [OI] > 25) were given a trial of iNO of 20 ppm for 20 minutes. Neonates who showed a greater than 20% improvement in PaO2 as well as a decrease in the OI to below 40 were defined as responders and continued on this therapy. RESULTS Four patterns of response emerged to the iNO therapy: Pattern 1 neonates (n = 2) did not respond to the initial trial of iNO-one survived. Pattern 2 neonates (n = 9) responded to the initial trial of iNO, but failed to sustain this response over 36 hours, as defined by a rise in the OI to > 40. Six survived, five with extracorporeal membrane oxygenation. Pattern 3 neonates (n = 11) responded to the initial trial of iNO, sustained this response, and were successfully weaned from iNO within 5 days--all survived to discharge. Pattern 4 neonates (n = 3) responded to the initial trial of iNO, but developed a sustained dependence on iNO for 3 to 6 weeks. All three died and lung histology revealed severe pulmonary hypoplasia and dysplasia. These neonates (pattern 4) not only required iNO for a longer period of time than did the sustained responders (pattern 3), but they required significantly higher doses of iNO during their first 5 days of iNO therapy. CONCLUSIONS Early responses to iNO may not be sustained. Neonates with pulmonary hypoplasia and dysplasia may have a decreased sensitivity and differing time course of response to iNO when compared with patients who have PPHN in fully developed lungs.
Protection against antigenically variable Borrelia burgdorferi conferred by recombinant vaccines

Due to local variation in the antigenicity of the agent of Lyme disease (Borrelia burgdorferi), a vaccine derived from any one isolate of this spirochete may fail to protect against the heterogeneous population of organisms that may be present in an enzootic focus. Accordingly, we determined whether antigenically variable spirochetes delivered by naturally infected ticks, collected from a site where transmission is intense, may fail to infect mice actively immunized with recombinant glutathione transferase outer surface fusion proteins A or B (OspA and OspB). Virtually all mice vaccinated by either immunogen appeared not to become infected, as determined by culture or histopathology of their tissues. We conclude that Osp vaccination of mice effectively prevents infection by the agent of Lyme disease in a simulated natural cycle of transmission.
A functional interaction between the putative primosomal protein DnaI and the main replicative DNA helicase DnaB in Bacillus.

In Gram negative Escherichia coli there are two well-characterised primosomal assembly processes, the PriA- and DnaA-mediated cascades. The presence of PriA and DnaA proteins in Gram positive Bacillus spp. supports the assumption that both the PriA- and DnaA-mediated primosomal assembly cascades also operate in these organisms. However, the lack of sequence homology between the rest of the primosomal proteins indicates significant differences between these two bacterial species. Central to the process of primosomal assembly is the loading of the main hexameric replicative helicase (DnaB in E.coli and DnaC in Bacillus subtilis) on the DNA. This loading is achieved by specialised proteins known as 'helicase loaders'. In E.coli DnaT and DnaC are responsible for loading DnaB onto the DNA during primosome assembly, in the PriA- and DnaA-mediated cascades, respectively. In Bacillus the identity of the helicase loader is still not established unequivocally. In this paper we provide evidence for a functional interaction between the primosomal protein DnaI from B.subtilis and the main hexameric replicative helicase DnaB from Bacillus stearothermophilus. Our results are consistent with the putative role of DnaI as the 'helicase loader' in the Gram positive Bacillus spp.
Common-Centroid FinFET Placement Considering the Impact of Gate Misalignment

The FinFET technology has been regarded as a better alternative among different device technologies at 22nm node and beyond due to more effective channel control and lower power consumption. However, the gate misalignment problem resulting from process variation based on the FinFET technology becomes even severer compared with the conventional planar CMOS technology. Such misalignment may increase the threshold voltage and decrease the drain current of a single transistor. When applying the FinFET technology to analog circuit design, the variation of drain currents will destroy the current matching among transistors and degrade the circuit performance. In this paper, we present the first FinFET placement technique for analog circuits considering the impact of gate misalignment together with systematic and random mismatch. Experimental results show that the proposed algorithms can obtain an optimized common-centroid FinFET placement with much better current matching.
Distinct Contributions of Ventromedial and Dorsolateral Subregions of the Human Substantia Nigra to Appetitive and Aversive Learning

The role of neurons in the substantia nigra (SN) and ventral tegmental area (VTA) of the midbrain in contributing to the elicitation of reward prediction errors during appetitive learning has been well established. Less is known about the differential contribution of these midbrain regions to appetitive versus aversive learning, especially in humans. Here we scanned human participants with high-resolution fMRI focused on the SN and VTA while they participated in a sequential Pavlovian conditioning paradigm involving an appetitive outcome (a pleasant juice), as well as an aversive outcome (an unpleasant bitter and salty flavor). We found a degree of regional specialization within the SN: Whereas a region of ventromedial SN correlated with a temporal difference reward prediction error during appetitive Pavlovian learning, a dorsolateral area correlated instead with an aversive expected value signal in response to the most distal cue, and to a reward prediction error in response to the most proximal cue to the aversive outcome. Furthermore, participants' affective reactions to both the appetitive and aversive conditioned stimuli more than 1 year after the fMRI experiment was conducted correlated with activation in the ventromedial and dorsolateral SN obtained during the experiment, respectively. These findings suggest that, whereas the human ventromedial SN contributes to long-term learning about rewards, the dorsolateral SN may be particularly important for long-term learning in aversive contexts. SIGNIFICANCE STATEMENT The role of the substantia nigra (SN) and ventral tegmental area (VTA) in appetitive learning is well established, but less is known about their contribution to aversive compared with appetitive learning, especially in humans. We used high-resolution fMRI to measure activity in the SN and VTA while participants underwent higher-order Pavlovian learning. We found a regional specialization within the SN: a ventromedial area was selectively engaged during appetitive learning, and a dorsolateral area during aversive learning. Activity in these areas predicted affective reactions to appetitive and aversive conditioned stimuli over 1 year later. These findings suggest that, whereas the human ventromedial SN contributes to long-term learning about rewards, the dorsolateral SN may be particularly important for long-term learning in aversive contexts.
Characterisation and pathological variability of Exserohilum turcicum responsible for causing northern corn leaf blight ( NCLB ) disease in Malaysia

Aims: Corn is grown primarily for human consumption. It is considered as the second most important cereal crop after rice in Asia. Many diseases affect this crop due to planting of susceptible hybrids. This research is aimed to characterize the causative agent of northern corn leaf blight disease in Malaysia, caused by Exserohilum turcicum. Methodology and results: Leaf samples were collected from infected farms of 2 corn growing areas of Peninsular Malaysia in 2015. A total of 5 fungal isolates were examined for cultural, morphological and molecular properties, and based on the results, the five isolates were identified as E. turcicum. The conidial shapes were observed to be elongated and spindle. Cultural characteristics showed that variation existed among the isolates in colony growth and colour. Mycelia growth rates of the isolates were significantly different on potato dextrose agar (PDA), corn meal agar (CMA) and potato sucrose agar (PSA) media, growth on CMA was faster than on PSA and PDA. The isolates were grouped into three groups based on colony colour i.e. light gray, gray and dark gray. The isolates were categorized into 2 groups based on growth namely, moderate growth and profused growth. The number of septa ranged from 5-7 to 7-10 representing isolates ET002 and ET003, respectively. Similarly, conidial length varied from 56.7 μm to 89.44 μm for isolates ET002 and ET003, respectively. The pathogenic variability tested on Thai Super Sweet (TSS) corn variety, showed that isolates ET001 and ET003 were more aggressive while isolate ET005 was less aggressive among the isolates tested. Conclusion, significance and impact of the study: Both morphology and molecular results showed that, the isolates were identified as E. turcicum. The findings of this study will serve as a baseline for future studies and will help to minimize losses in yield.
A Sentiment Analyzer for Hindi Using Hindi Senti Lexicon

Supervised approaches have proved their signiﬁcance in sentiment analysis task, but they are limited to the languages, which have sufﬁcient amount of annotated corpus. Hindi is a language, which is spoken by 4 . 70% of the world population, but it lacks a sufﬁcient amount of annotated corpus for natural language processing tasks such as Sentiment Analysis (SA). With the increase in demand and availability of Hindi review websites, an accurate sentiment analyzer for Hindi has become a need. In this paper, we present a bootstrap approach to extract senti words from Hindi-WordNet. The approach is designed such that it minimizes the extraction of the words with the wrong polarity orientation, which is a crucial task, because a word can have positive and negative senses at the same time. The resultant set of 8061 polar words, we call it Hindi senti lexicon, is used for sentiment analysis in Hindi. We get an average accuracy of 87% for sentiment analysis in the movie and product domain.
Nitrate Reductase from the Marine Diatom Skeletonema costatum (Biochemical and Immunological Characterization)

Assimilatory nitrate reductase (NR) was purified from the marine diatom Skeletonema costatum (clone Skel) using Cibacron blue-Sepharose affinity chromatography. The single-step purification scheme yielded a 103-fold purification of specific activity with an overall recovery of 40.8%. Only NADH-dependent NR activity (form EC 1.6.6.1) was observed in this species. Kinetic analysis revealed that this form had apparent Michaelis constants of 3.6 [mu]M for NADH and 295 [mu]M for NO3- when purified from cells grown in NO3--enriched seawater. The S. costatum NR exhibits a pH optimum of 7.4, a temperature optimum of 14[deg]C, and enzyme activity not sensitive to Mg2+ inhibition. The strong temperature dependence of NR activity in S. costatum may contribute to the seasonal and latitudinal distributions and abundances of this bloom-forming species. Chromatographically isolated NR was further purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, yielding a single polypeptide with an apparent molecular mass of 110 kD. The 110-kD polypeptide was used to generate polyclonal antibodies. The antiserum recognized a single 110-kD polypeptide in western blots of total proteins from S. costatum, as well as the native enzyme. Western blot analysis also revealed an antigenic similarity of NR from two additional diatom species, whereas no cross-reactivity was observed with NR from other phytoplankton taxa, including prymnesiophytes, dinoflagellate, cyanobacterium, and green alga. This result suggests a structural diversity of NR in phytoplankton and identifies the potential for development of taxon-specific NR antisera for ecological studies.
Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli

A major portion of tetracycline accumulation by susceptible bacterial cells is energy dependent. Inner membrane vesicles prepared from susceptible Escherichia coli cells concentrated tetracycline 2.5 to 5 times above the external concentration when the electron transport substrate D-lactate or reduced phenazine methosulfate was added. This stimulation was reversed by cyanide, 2,4-dinitrophenol, and carbonyl cyanide m-chlorophenyl hydrazone. These vesicles data showed that proton motive force alone could energize tetracycline uptake. The lactate-dependent uptake had a pH optimum of 6.9 and a magnesium optimum of 1 mM and was not saturable up to 400 microM tetracycline. Although the vesicles were not as active as cells in concentrating tetracycline, they were less active to a similar extent in concentrating tetracycline, they were less active to a similar extent in concentrating proline, the transport of which is known to be solely proton motive force dependent. Therefore, we concluded that the active uptake of tetracycline in susceptible cells was largely, if not solely, energized by proton motive force.
Temperature Significantly Affects the Plaquing and Adsorption Efficiencies of Listeria Phages

Listeria-infecting phages are currently being used to control and detect the important foodborne pathogen Listeria monocytogenes; however, the influence of environmental conditions on the interactions between L. monocytogenes and its phages has not been explored in depth. Here, we examined the infective potential of four Listeria phages (two each from the P70-like and P100-like phages of Listeria) against five strains of L. monocytogenes (representing serotypes 1/2a, 1/2b, 4a, and 4b) grown under a range of temperatures (7–37°C). We show that the plaquing efficiencies for all four phages were significantly affected by temperature. Interestingly, no plaques were observed for any of the four phages at 37°C. Adsorption assays performed with the P100-like phages, LP-048 and LP-125, showed that LP-048 had a severely reduced adsorption efficiency against susceptible strains at 37°C as compared to 30°C, suggesting that there is considerably less accessible rhamnose (LP-048’s putative phage receptor) on the host at 37°C than at 30°C. LP-125 adsorbed to host cells at 37°C, indicating that the inability for LP-125 to plaque at 37°C is not due to adsorption inhibition. LP-048 showed significantly higher adsorption efficiency against a mutant strain lacking N-acetylglucosamine in its wall teichoic acids (WTA) than the parental strain at both 30 and 37°C, suggesting that N-acetylglucosamine competes with rhamnose for glycosylation sites on the WTA. The data presented here clearly shows that L. monocytogenes can gain physiological refuge from phage infection, which should be carefully considered for both the design and implementation of phage-based control and detection applications.
Efficient Bayesian Clustering for Reinforcement Learning

A fundamental artificial intelligence challenge is how to design agents that intelligently trade off exploration and exploitation while quickly learning about an unknown environment. However, in order to learn quickly, we must somehow generalize experience across states. One promising approach is to use Bayesian methods to simultaneously cluster dynamics and control exploration; unfortunately, these methods tend to require computationally intensive MCMC approximation techniques which lack guarantees. We propose Thompson Clustering for Reinforcement Learning (TCRL), a family of Bayesian clustering algorithms for reinforcement learning that leverage structure in the state space to remain computationally efficient while controlling both exploration and generalization. TCRL-Theoretic achieves near-optimal Bayesian regret bounds while consistently improving over a standard Bayesian exploration approach. TCRL-Relaxed is guaranteed to converge to acting optimally, and empirically outperforms state-of-the-art Bayesian clustering algorithms across a variety of simulated domains, even in cases where no states are similar.
Physiological response of maize (Zea mays L.) to high temperature stress.

This study was conducted to investigate physiological response of maize to high temperature stress. Field trial was carried out in a randomized block design with three replications during the second crop season of the year 2008 in Sanliurfa, Turkey. RI, chlorophyll content, and Chla/b were measured in laboratory. LT, DF, and GY were measured in field trial. The relations among the investigated characteristics were evaluated by correlation analysis. We found that early mature varieties had higher values with regard to chlorophyll content and GY compared to late mature varieties. However, early mature varieties had lower values with regard to RI, LT, and DF than late mature varieties. Correlation analysis revealed significant relations among the characteristics measured. It was concluded that early mature varieties having lower LT and RI levels compared to late mature varieties might be recommended as second crop for the region with extremely high temperatures during the second crop season.
Effects of 15d‐PGJ2‐loaded poly(D,L‐lactide‐co‐glycolide) nanocapsules on inflammation

The PPAR‐γ agonist 15d‐PGJ2 is a potent anti‐inflammatory agent but only at high doses. To improve the efficiency of 15d‐PGJ2, we used poly(D,L‐lactide‐co‐glycolide) nanocapsules to encapsulate it, and function as a drug carrier system. The effects of these loaded nanocapsules (15d‐PGJ2‐NC) on inflammation induced by different stimuli were compared with those of free 15d‐PGJ2.
The Subtelomeric khipu Satellite Repeat from Phaseolus vulgaris: Lessons Learned from the Genome Analysis of the Andean Genotype G19833

Subtelomeric regions in eukaryotic organisms are known for harboring species-specific tandemly repeated satellite sequences. However, studies on the molecular organization and evolution of subtelomeric repeats are scarce, especially in plants. Khipu is a satellite DNA of 528-bp repeat unit, specific of the Phaseolus genus, with a subtelomeric distribution in common bean, P. vulgaris. To investigate the genomic organization and the evolution of khipu, we performed genome-wide analysis on the complete genome sequence of the common bean genotype G19833. We identified 2,460 khipu units located at most distal ends of the sequenced regions. Khipu units are arranged in discrete blocks of 2–55 copies and are heterogeneously distributed among the different chromosome ends of G19833 (from 0 to 555 khipus units per chromosome arm). Phylogenetically related khipu units are spread between numerous chromosome ends, suggesting frequent exchanges between non-homologous subtelomeres. However, most subclades contain numerous khipu units from only one or few chromosome ends indicating that local duplication is also driving khipu expansion. Unexpectedly, we also identified 81 khipu units located at centromeres. All the centromeric khipu units belong to a single divergent clade also comprised of a few units from several subtelomeres, suggesting that a few sequence exchanges between centromeres and subtelomeres took place in the common bean genome. The divergence and low copy number of these centromeric units from the subtelomeric units could explain why they were not detected by FISH (Fluorescence in situ Hybridization) although it can not be excluded that these centromeric units may have resulted from errors in the pseudomolecule assembly. Altogether our data highlight extensive sequence exchanges in subtelomeres between non-homologous chromosomes in common bean and confirm that subtelomeres represent one of the most dynamic and rapidly evolving regions in eukaryotic genomes.
Protective effects of sugar cane extracts (SCE) on Eimeria tenella infection in chickens.

The effects of oral administration of sugar cane extracts (SCE) on Eimeria tenella oocysts infection in chickens were studied with 2 different experiments. In Experiment 1, 3-week-old inbred chickens (MHC; H.B15) were inoculated into the crop with SCE (500 mg/kg/day) for 1 day or 3 consecutive days, and then challenged with E. tenella sporulated oocysts (2 x 10(4) cells/chicken). In Experiment 2, 1-week-old chickens were orally administered SCE at the same dose for 3 consecutive days, and then initially infected with E. tenella sporulated oocysts (2 x 10(3) cells/chicken). At 2 and 3 weeks of age, these chickens were immunized intravenously with the mixed antigens of sheep red blood cells (SRBC) and Brucella abortus (BA). At 4 weeks of age, chickens were challenged with E. tenella sporulated oocysts (1 x 10(5)/chicken). Challenged chickens with E. tenella oocysts showed markedly decreased body weight gain/day, severe hemorrhage and great number of shedding oocysts in feces and high lesion scores. Oral administration of SCE and initial infection with oocysts (2 x 10 (3)/chicken) resulted in a remarkable improvement in body weight gain/day, hemorrhage, the number of shedding oocysts and lesion score, compare to other infected groups. In addition, SCE-inoculated chickens with the initial infection showed a significant increase in antibody responses against SRBC and BA and also improvement in decreased relative proportions of Bu-1a(+) and CD4( )cells in cecal tonsil lymphocytes of E. tenella-challenged chickens. Cecal tissues of chickens administered SCE and initially infected with E. tenella oocysts showed lower numbers of schizonts, gametocytes and oocysts than those of infected control chickens. These results suggest that SCE have immunostimulating and protective effects against E. tenella infection in chickens.