Miracle-dz/UniFAR-FTUs huggingface dataset:
When eye meets ear : an investigation of audiovisual speech and non-speech perception in younger and older adults

This dissertation addressed important questions regarding audiovisual (AV) perception. Study 1 revealed that AV speech perception modulated auditory processes, whereas AV non-speech perception affected visual processes. Interestingly, stimulus identification improved, yet fewer neural resources, as reflected in smaller event-related potentials, were recruited, indicating that AV perception led to multisensory efficiency. Also, AV interaction effects were observed of early and late stages, demonstrating that multisensory integration involved a neural network. Study 1 showed that multisensory efficiency is a common principle in AV speech and non-speech stimulus recognition, yet it is reflected in different modalities, possibly due to sensory dominance of a given task. Study 2 extended our understanding of multisensory interaction by investigating electrophysiological processes of AV speech perception in noise and whether those differ between younger and older adults. Both groups revealed multisensory efficiency. Behavioural performance improved while the auditory N1 amplitude was reduced during AV relative to unisensory speech perception. This amplitude reduction could be due to visual speech cues providing complementary information, therefore reducing processing demands for the auditory system. AV speech stimuli also led to an N1 latency shift, suggesting that auditory processing was faster during AV than during unisensory trials. This shift was more pronounced in older than in younger adults, indicating that older adults made more effective use of visual speech. Finally, auditory functioning predicted the degree of the N1 latency shift, which is consistent with the inverse effectiveness hypothesis which argues that the less effective the unisensory perception was, the larger was the benefit derived from AV speech cues. These results suggest that older adults were better "lip/speech" integrators than younger adults, possibly to compensate for age-related sensory deficiencies. Multisensory efficiency was evident in younger and older adults but it might be particularly relevant for older adults. If visual speech cues could alleviate sensory perceptual loads, the remaining neural resources could be allocated to higher level cognitive functions. This dissertation adds further support to the notion of multisensory interaction modulating sensory-specific processes and it introduces the concept of multisensory efficiency as potential principle underlying AV speech and non-speech perception
A Multiphase Class-D Automotive Audio Amplifier With Integrated Low-Latency ADCs for Digitized Feedback After the Output Filter

A <inline-formula> <tex-math notation="LaTeX">$5 \times 80$ </tex-math></inline-formula> W class-D audio power amplifier for automotive applications is presented. The amplifier is implemented in a 140-nm bipolar CMOS DMOS SOI. Configurable digital-loop filters can compensate for a range of LC output filters and their high loop gain (>50 dB between 20 Hz and 20 kHz) suppresses non-idealities of the output filter and enables low-cost output filter components. Key elements are the integrated low-latency <inline-formula> <tex-math notation="LaTeX">$\Delta \Sigma$ </tex-math></inline-formula> analog to digital converters (ADCs) which digitize the output signals directly at the speaker load, after the output filter. The ADCs use filtering finite impulse response digital to analog converters in their feedback path to create an input–output transfer with a negative group delay at low frequencies. The ADCs have 116-dBA DR and −108 dB total harmonic distortion (THD). The bridge-tied load amplifier supports multiphase pulse-width modulation for lower electromagnetic interference. It operates with supplies from 6 to 25 V and with loads down to <inline-formula> <tex-math notation="LaTeX">$1\Omega$ </tex-math></inline-formula> and achieves 19-<inline-formula> <tex-math notation="LaTeX">$\mu \text{V}$ </tex-math></inline-formula> idle noise (Awtd) and 0.004% THD + N.
ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer.

BACKGROUND Integrative and conjugative elements (ICEs) have not been detected in Pasteurella multocida. In this study the multiresistance ICEPmu1 from bovine P. multocida was analysed for its core genes and its ability to conjugatively transfer into strains of the same and different genera. METHODS ICEPmu1 was identified during whole genome sequencing. Coding sequences were predicted by bioinformatic tools and manually curated using the annotation software ERGO. Conjugation into P. multocida, Mannheimia haemolytica and Escherichia coli recipients was performed by mating assays. The presence of ICEPmu1 and its circular intermediate in the recipient strains was confirmed by PCR and sequence analysis. Integration sites were sequenced. Susceptibility testing of the ICEPmu1-carrying recipients was conducted by broth microdilution. RESULTS The 82 214 bp ICEPmu1 harbours 88 genes. The core genes of ICEPmu1, which are involved in excision/integration and conjugative transfer, resemble those found in a 66 641 bp ICE from Histophilus somni. ICEPmu1 integrates into a tRNA(Leu) and is flanked by 13 bp direct repeats. It is able to conjugatively transfer to P. multocida, M. haemolytica and E. coli, where it also uses a tRNA(Leu) for integration and produces closely related 13 bp direct repeats. PCR assays and susceptibility testing confirmed the presence and the functional activity of the ICEPmu1-associated resistance genes in the recipient strains. CONCLUSIONS The observation that the multiresistance ICEPmu1 is present in a bovine P. multocida and can easily spread across strain and genus boundaries underlines the risk of a rapid dissemination of multiple resistance genes, which will distinctly decrease the therapeutic options.
Aerobic Heterotrophic Bacterial Populations of Sewage and Activated Sludge

The replica-plating technique and Lochhead's nutritional method were combined in exploratory experiments to test their feasibility as useful means for characterizing the aerobic heterotrophic flora of activated sludge and to minimize the burdensome process of isolation, purification, and testing of isolates. In the test run, the method was about 86% reliable at the 0.05 level of significance. About 40% of the total number of bacteria able to grow on an aqueous extract of activated sludge did not grow on media containing glucose, amino acids, growth factors, and inorganic salts. The requirement for activated sludge extract suggested the existence of a requirement for unidentified nutrients contained in the activated sludge extract.
Journeys to school leadership

The need to identify and suitably prepare teachers to undertake school leadership roles especially as principals is now well documented in the literature. Similarly documented is the general concern about the lack of suitable applicants willing to consider the role. This study raised the question of what might be learnt when a purposefully-selected cohort of 31 teachers drawn from executive, non-principal roles participated in a year-long, multi-phased leadership development program: The Flagship Program of the Association of Independent Schools of New South Wales, Australia (AIS). The design of the program was informed by internationally acknowledged principles of good practice in leadership preparation. Evaluation of the program combined action learning methodologies working with participants with more formal evaluation of each of the program’s stages to investigate participant growth and program effectiveness. The action learning component undertaken with the direct involvement of all participants and with the support of the Australian Institute for Teaching and School Leadership (AITSL) was able to pinpoint what the participants themselves saw as important and what was less so at this stage of their preparation for school leadership roles. In so doing, the study adds a valuable participant perspective to the overall literature on leadership preparation.
Real-Time Classification of Complex Patterns Using Spike-Based Learning in Neuromorphic VLSI

Real-time classification of patterns of spike trains is a difficult computational problem that both natural and artificial networks of spiking neurons are confronted with. The solution to this problem not only could contribute to understanding the fundamental mechanisms of computation used in the biological brain, but could also lead to efficient hardware implementations of a wide range of applications ranging from autonomous sensory-motor systems to brain-machine interfaces. Here we demonstrate real-time classification of complex patterns of mean firing rates, using a VLSI network of spiking neurons and dynamic synapses which implement a robust spike-driven plasticity mechanism. The learning rule implemented is a supervised one: a teacher signal provides the output neuron with an extra input spike-train during training, in parallel to the spike-trains that represent the input pattern. The teacher signal simply indicates if the neuron should respond to the input pattern with a high rate or with a low one. The learning mechanism modifies the synaptic weights only as long as the current generated by all the stimulated plastic synapses does not match the output desired by the teacher, as in the perceptron learning rule. We describe the implementation of this learning mechanism and present experimental data that demonstrate how the VLSI neural network can learn to classify patterns of neural activities, also in the case in which they are highly correlated.
A Sensitive Immunoblotting Technique for the Serodiagnosis of Brucella Ovis Infections

A simplified electrophoretic immunoblotting technique based on antigen extracted from Brucella ovis cells with sodium dodecyl sulfate/mercaptoethanol was compared with the complement fixation test (CFT), the enzyme-linked immunosorbent assay, and the gel diffusion test. Sera from 89 chronically infected, semen culture-positive rams, 378 sera from B. ovis-infected flocks, 300 sera from accredited disease-free flocks, and 29 sera from specific-pathogen-free sheep were used. The immunoblotting technique had sensitivity and specificity comparable to those of the standard tests and was able to identify several CFT-negative or -borderline sera as positive. The major immunoreactive antigens of B. ovis had molecular masses of 63,29, 19 kD (proteins) and 8-12 kD (rough lipopolysaccharide). Antibodies against these antigens were present in 96% of CFT-positive sera from infected flocks and in 100% of sera from semen culture-positive rams. However, immunoblotting also identified antibodies to components other than the major antigens in 1% of CFT-negative sera from infected flocks and in 7.7% of the sera from flocks with a history of freedom from the disease. These reactions probably represent cross-reactivities with other microorganisms and were distinguishable from truly positive reactions.
HIV-1 in genital tract and plasma of women: Compartmentalization of viral sequences, coreceptor usage, and glycosylation

Worldwide, 90% of HIV-1 infections are transmitted heterosexually. Because the genital mucosa are the sites of initial contact with HIV-1 for most exposed individuals, study of the virus from the genital tract is critical for the development of vaccines and therapeutics. Previous analyses of HIV-1 in various tissues have documented compartmentalization of viral genomes. Whether compartmentalization was associated with viral phenotypic differences or immune status, however, was not well understood. We compared HIV-1 gp120 env sequences from the genital tract and plasma of 12 women. Eight women displayed compartmentalized HIV-1 RNA genomes, with viral sequences from each site that were clearly discrete, yet phylogenetically related. The remaining four exhibited env sequences that were intermingled between the two sites. Women with compartmentalized HIV-1 genomes had higher CD4+ cell counts than those displaying intermingled strains (P = 0.02). Intrapatient HIV-1 recombinants comprising sequences that were characteristic of both sites were identified. We next compared viral phenotypes in each compartment. HIV-1 coreceptor usage was often compartmentalized (P ≤ 0.01). The number of N-linked glycosylation sites, associated with neutralization resistance, also differed between compartments (P < 0.01). Furthermore, disparities between the density of gp120 glycosylations in each compartment correlated with higher CD4+ counts (P = 0.03). These data demonstrate that the genital tract and plasma can harbor populations of replicating HIV-1 with different phenotypes. The association of higher CD4+ cell counts with compartmentalization of viral genomes and density of gp120 glycosylations suggests that the immune response influences the development of viral genotypes in each compartment. These findings are relevant to the prevention and control of HIV-1 infection.
Surprisingness and Expectation Failure: What's the Difference?

Intelligent systems operate in the midst of a superabundance of information lacking the tags that indicate which few aspects are significant to the particular problems at hand at any given time and place. Given this wealth of information coupled with real-time processing constraints, selective attention is fundamental to any chance of success. In much of cognitive science, attentional focus is linked with the concept of expectation generation and failure. Unfortunately, research in AI has to finesse the problem of selective attention, although sometimes recognizing it as an important component of the frame problem (e.g., Schank, 1979; 1982).
Fuzzy Information Granules: a Compact, Transparent and Efficient Representation

1 This paper presents a method to construct information granules that provide a relevant description of experimental observations and, at the same time, are represented in a compact and semantically sound form. The method works by first granulating data through a fuzzy clustering algorithm, and then representing granules in form of fuzzy sets. Specifically, an optimal Gaussian functional form for the membership functions is derived by solving a constrained optimization problem on the membership values of the partition matrix returned by the clustering algorithm. The granules represented with Gaussian functional forms can be used to build a fuzzy inference system that performs inferences on the working environment. To illustrate the behavior of the proposed method a real-world information granulation problem has been used. Simulation results show that compact and robust fuzzy granules are attained, with the appreciable feature of being represented in a short functional form. In addition to the information granulation problem, a descriptive fuzzy model for a prediction benchmark has been developed to verify how much fuzzy granules identified form data through the proposed method are useful in providing good mapping properties. The obtained results are reported, supported by comparison with other works.
Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling

Notch signaling plays a crucial role in adult brain function such as synaptic plasticity, memory and olfaction. Several reports suggest an involvement of this pathway in neurodegenerative dementia. Yet, to date, the mechanism underlying Notch activity in mature neurons remains unresolved. In this work, we investigate how Notch regulates synaptic potentiation and contributes to the establishment of memory in mice. We observe that Notch1 is a postsynaptic receptor with functional interactions with the Reelin receptor, apolipoprotein E receptor 2 (ApoER2) and the ionotropic receptor, N-methyl-D-aspartate receptor (NMDAR). Targeted loss of Notch1 in the hippocampal CA fields affects Reelin signaling by influencing Dab1 expression and impairs the synaptic potentiation achieved through Reelin stimulation. Further analysis indicates that loss of Notch1 affects the expression and composition of the NMDAR but not AMPAR. Glutamatergic signaling is further compromised through downregulation of CamKII and its secondary and tertiary messengers resulting in reduced cAMP response element-binding (CREB) signaling. Our results identify Notch1 as an important regulator of mechanisms involved in synaptic plasticity and memory formation. These findings emphasize the possible involvement of this signaling receptor in dementia. Highlights In this paper, we propose a mechanism for Notch1-dependent plasticity that likely underlies the function of Notch1 in memory formation: Notch1 interacts with another important developmental pathway, the Reelin cascade. Notch1 regulates both NMDAR expression and composition. Notch1 influences a cascade of cellular events culminating in CREB activation.
Potential New Antiepileptogenic Targets Indicated by Microarray Analysis in a Rat Model for Temporal Lobe Epilepsy

To get insight into the mechanisms that may lead to progression of temporal lobe epilepsy, we investigated gene expression during epileptogenesis in the rat. RNA was obtained from three different brain regions [CA3, entorhinal cortex (EC), and cerebellum (CB)] at three different time points after electrically induced status epilepticus (SE): acute phase [group D (1 d)], latent period [group W (1 week)], and chronic epileptic period [group M (3–4 months)]. A group that was stimulated but that had not experienced SE and later epilepsy was also included (group nS). Gene expression analysis was performed using the Affymetrix Gene Chip System (RAE230A). We used GENMAPP and Gene Ontology to identify global biological trends in gene expression data. The immune response was the most prominent process changed during all three phases of epileptogenesis. Synaptic transmission was a downregulated process during the acute and latent phases. GABA receptor subunits involved in tonic inhibition were persistently downregulated. These changes were observed mostly in both CA3 and EC but not in CB. Rats that were stimulated but that did not develop spontaneous seizures later on had also some changes in gene expression, but this was not reflected in a significant change of a biological process. These data suggest that the targeting of specific genes that are involved in these biological processes may be a promising strategy to slow down or prevent the progression of epilepsy. Especially genes related to the immune response, such as complement factors, interleukins, and genes related to prostaglandin synthesis and coagulation pathway may be interesting targets.
Medical students' views on training in intellectual disabilities.

OBJECTIVE To examine undergraduate medical training in the field of intellectual disabilities (ID) from the perspective of clinical clerks. DESIGN Cross-sectional self-administered survey. SETTING Clerkship rotations at Queen's University in Kingston, Ont, and the University of Toronto in Ontario in 2006. PARTICIPANTS A total of 196 upper-year undergraduate medical students (clerks). MAIN OUTCOME MEASURES Contact with people with ID, training in the field of ID, perceptions of current training in ID, and views on ways to improve the curriculum in the area of ID. RESULTS Most students (85.6%) had received some didactic and clinical training in managing patients with ID,but most of these (93.3%) believed that curriculum enhancements, especially more contact with patients with ID and more time in the curriculum for training in care of people with ID, were necessary. CONCLUSION This study found that the curriculum enhancements long recommended by experts in the field of ID were also desired by clinical clerks. This finding adds considerable weight to the recommendation that improvements in training in ID should be incorporated into undergraduate medical education programs.
Database Search Based on Bayesian Alignment

The size of protein sequence database is getting larger each day. One common challenge is to predict protein structures or functions of the sequences in databases. It is easy when a sequence shares direct similarity to a well-characterized protein. If there is no direct similarity, we have to rely on a third sequence or a model as intermediate to link two proteins together. We developed a new model based method, called Bayesian search, as a means to connect two distantly related proteins. We compared this Bayesian search model with pairwise and multiple sequence comparison methods on structural databases using structural similarity as the criteria for relationship. The results show that the Bayesian search can link more distantly related sequence pairs than other methods, collectively and consistently over large protein families. If each query made one error on average against SCOP database PDB40D-B, Bayesian search found 36.5% of related pairs, PSI-Blast found 32.6%, and Smith-Waterman method found 25%. Examples are presented to show that the alignments predicted by the Bayesian search agree well with structural alignments. Also false positives found by Bayesian search at low cutoff values are analyzed.
Analysis of the rapid responses of Nereis and Harmothoë ( Annelida )

The giant-fibre responses of Harmothoë and Nereis have been studied with emphasis on the afferent and efferent path ways and the sites of the rapid accommodation of the fast response. When the bundle of sensory neurones of the anal cirrus is stimulated the giant-fibres respond at the first shock. This terminal junction then rapidly accommodates to further afferent excitation. In Harmothoë the muscles which effect the rapid movements of the giant-fibre response are directly innervated by large axons of unipolar cell bodies in the central nervous system. In each segment one neurone of this type supplies both dorsal and ventral longitudinal muscle fibres. By use of a bridge technique this neurone has been isolated. At the first stimulus above a single sharp threshold the resulting nerve muscle preparation gives a maximum electrical response, which is independent of the stimulus strength. A reduced response persists for many repetitions at low frequency. In addition to this fast motor neurone a slower system in the same muscles is indicated by the muscle-action potentials and by observation of the movements. An axon-axon synapse seen histologically between the lateral giant fibres and the large motor neurone to the longitudinal muscle has been identified with the rapidly accommodating physiological junction between these elements. At this level of analysis the afferent and efferent relations of the giant fibres, and in particular the fast motor innervation, are broadly comparable with those of some arthropods.
Assessing the Difficulty of Chess Tactical Problems

We investigate experts’ ability to assess the difficulty of a mental task for a human. The final aim is to find formalized measures of difficulty that could be used in automated assessment of the difficulty of a task. In experiments with tactical chess problems, the experts’ estimations of difficulty are compared to the statistic-based difficulty ratings on the Chess Tempo website. In an eye tracking experiment, the subjects’ solutions to chess problems and the moves that they considered are analyzed. Performance data (time and accuracy) are used as indicators of subjectively perceived difficulty. We also aim to identify the attributes of tactical positions that affect the difficulty of the problem. Understanding the connection between players’ estimation of difficulty and the properties of the search trees of variations considered is essential, but not sufficient, for modeling the difficulty of tactical problems. Our findings include that (a) assessing difficulty is also very difficult for human experts, and (b) algorithms designed to estimate difficulty should interpret the complexity of a game tree in the light of knowledge-based patterns that human players are able to detect in a chess problem. Keywords–Task Difficulty; Assessing Problem Difficulty; Eye Tracking; Problem Solving; Chess Tactical Problems; Chess