Miracle-dz/UniFAR-FTUs huggingface dataset:
Pre-analytical and analytical issues in the analysis of blood microparticles

Summary Results of plasma microparticles (MPs) measurements reported in the literature vary widely. This is clearly not only related to the lack of well-standardised MP assays, but also to variations in pre-analytical conditions. In this review we will discuss the pre-analytical variables related to plasma and MP preparation which may affect MP analysis. Additionally we will address several analytical issues in commonly used MP as-says and briefly discuss some novel approaches for the detection and characterisation of MPs. Ideally MP measurements should be performed in plasma, freshly prepared directly after blood withdrawal. As platelet contamination seems to be one of the major pre-analytical problems in processing plasma for MP measurement, the use of platelet-free plasma may be preferred. When frozen-thawed plasma is used, especially PMP and annexinV-positive MP counts should be interpreted with caution. When flow cytometry is chosen as a method for quantifi-cation of MPs, some analytical conditions should be standardised, e.g. settings of the flow cytometer, quality of the antibodies, and use of counting beads. Fluorescence-nanoparticle tracking analysis and atomic force microscopy can accurately count nanosized MPs, but unfortunately the operational procedures of both methods are still time consuming and they give no information on the functional properties of MPs. The MP-TF activity assay provides information on MPs carrying active TF, regardless of their parental origin. Ultimately, standardisation of pre-analytical procedures and the introduction of reliable and rapid methods for the measurement of MPs are urgently needed to facilitate their use as biomarker in the pathophysiology of diseases.
The Effect of Metformin on Adolescents with Type 1 Diabetes: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Background. The effect of metformin in combination with insulin in adolescents with type 1 diabetes (T1DM) is controversial. Methods and Results. The PubMed and EMBASE online databases were searched. Five double-blind randomized controlled trials (RCTs) that included 301 adolescents with T1DM were identified. Metformin plus insulin was associated with reduced hemoglobin A1C levels, total daily insulin dosage, body mass index (BMI), and body weight. However, the subgroup analysis demonstrated that HbA1c levels were not significantly changed in overweight/obese adolescents and were significantly reduced in the general patients. On the contrary, BMI and body weight were significantly reduced in overweight/obese adolescents but not in the general patients. Metformin was associated with higher incidence of adverse events. Conclusions. Among adolescents with T1DM, administering adjunctive metformin therapy in addition to insulin was associated with improved HbA1c levels, total daily insulin dosage, BMI, and body weight. However, there may be differences in the effects of this regimen between overweight/obese and nonobese adolescents. The risk of an adverse event may be increased with metformin treatment. These results provide strong evidence supporting future high-quality, large-sample trials.
Non-Asymptotic Achievable Rates for Energy-Harvesting Channels Using Save-and-Transmit

This paper investigates the information-theoretic limits of energy-harvesting (EH) channels in the finite blocklength regime. The EH process is characterized by a sequence of i.i. d. random variables with finite variances. We use the save-andtransmit strategy proposed by Ozel and Ulukus (2012) together with Shannon's non-asymptotic achievability bound to obtain lower bounds on the achievable rates for both additive white Gaussian noise channels and discrete memoryless channels under EH constraints. The first-order terms of the lower bounds of the achievable rates are equal to C and the second-order (backoff from capacity) terms are proportional to -√log n/n, where n denotes the blocklength and C denotes the capacity of the EH channel, which is the same as the capacity without the EH constraints. The constant of proportionality of the backoff term is found and qualitative interpretations are provided.
T-Helper Type-2 Contact Hypersensitivity of Balb/c Mice Aggravated by Dibutyl Phthalate via Long-Term Dermal Exposure

Objective During the last few decades, the prevalence of allergic skin diseases, asthma and rhinitis, has increased worldwide. Introduction of environmental chemicals with aggravation effects may play a part in this increase. The artificial chemical product dibutyl phthalate (DBP) is used in many products used in daily life. Dermal exposure to DBP is a common (but easily neglected) exposure pattern. Methodology/Principal Findings In this study, we examined the aggravation effect of long-term dermal exposure to DBP in a T-helper type 2 (Th2) model of contact hypersensitivity (CHS) in mice, and sought the potential molecular mechanisms. Experimental tests were conducted after 40-day dermal exposure to saline or three concentrations of DBP and subsequent three times of sensitization with 0.5% fluorescein isothiocyanate (FITC) or vehicle. The results of immunological and inflammatory biomarkers (total-immunoglobulin (Ig)E and Th cytokines) as well as histopathological examination and measurement of ear swelling supported the notion that high doses of DBP may promote and aggravate atopic dermatitis. Increased expression of thymic stromal lymphopoietin (TSLP) in this mouse model suggested that TSLP might be one of the molecular mechanisms of the aggravation effect induced by DBP. Conclusions/Significance Together, these results indicated that long-term dermal exposure to types of environmental toxins such as phthalates may endow an atopic predisposition in animals or humans. In addition, the high expression of TSLP in the mouse model demonstrated that TSLP might have an important role in the aggravation effect. This result could help to provide effective prevention strategies against atopic diseases such as atopic dermatitis (AD).
Rac1 Regulates Stress-induced, Redox-dependent Heat Shock Factor Activation*

The signaling pathway by which environmental stresses activate heat shock factors (HSFs) is not completely understood. We show that the small GTPase rac1, and Rac1-regulated reactive oxygen species (ROS) play an important role in stress-stimulated heat shock response. A dominant-negative allele of Rac1 (Rac1N17) inhibits the hypoxia/reoxygenation and sodium arsenite-induced transcriptional activity of HSF-1 and the transcription of heat shock protein 70. Rac1N17 also suppresses the production of intracellular ROS induced by hypoxia/reoxygenation or sodium arsenite. Moreover, direct suppression of intracellular ROS levels by antioxidants decreases stress-stimulated HSF activity. However, expression of a constitutively active mutant of Rac1 (Rac1V12) in the absence of extracellular stresses does not increase intracellular ROS levels or induce the heat shock response. These results show that Rac1 is a necessary but insufficient component of the stress-induced signaling pathway that leads to ROS production, activation of HSFs, and transcription of heat shock proteins.
Responsibility and the sense of agency enhance empathy for pain

Being held responsible for our actions strongly determines our moral judgements and decisions. This study examined whether responsibility also influences our affective reaction to others' emotions. We conducted two experiments in order to assess the effect of responsibility and of a sense of agency (the conscious feeling of controlling an action) on the empathic response to pain. In both experiments, participants were presented with video clips showing an actor's facial expression of pain of varying intensity. The empathic response was assessed with behavioural (pain intensity estimation from facial expressions and unpleasantness for the observer ratings) and electrophysiological measures (facial electromyography). Experiment 1 showed enhanced empathic response (increased unpleasantness for the observer and facial electromyography responses) as participants' degree of responsibility for the actor's pain increased. This effect was mainly accounted for by the decisional component of responsibility (compared with the execution component). In addition, experiment 2 found that participants' unpleasantness rating also increased when they had a sense of agency over the pain, while controlling for decision and execution processes. The findings suggest that increased empathy induced by responsibility and a sense of agency may play a role in regulating our moral conduct.
Measuring neural representations with fMRI: practices and pitfalls

Recently, there has been a dramatic increase in the number of functional magnetic resonance imaging studies seeking to answer questions about how the brain represents information. Representational questions are of particular importance in connecting neuroscientific and cognitive levels of analysis because it is at the representational level that many formal models of cognition make distinct predictions. This review discusses techniques for univariate, adaptation, and multivoxel analysis, and how they have been used to answer questions about content specificity in different regions of the brain, how this content is organized, and how representations are shaped by and contribute to cognitive processes. Each of the analysis techniques makes different assumptions about the underlying neural code and thus differ in how they can be applied to specific questions. We also discuss the many pitfalls of representational analysis, from the flexibility in data analysis pipelines to emergent nonrepresentational relationships that can arise between stimuli in a task.
Differential methylation of E2 binding sites in episomal and integrated HPV 16 genomes in preinvasive and invasive cervical lesions

Enhanced expression of the HPV 16 E6‐E7 oncogenes may trigger neoplastic transformation of the squamous epithelial cells at the uterine cervix. The HPV E2 protein is a key transcriptional regulator of the E6‐E7 genes. It binds to four E2 binding sites (E2BSs 1–4) in the viral upstream regulatory region (URR). Modification of E2 functions, for example, by methylation of E2BSs is hypothesized to trigger enhanced expression of the viral E6‐E7 oncogenes. In the majority of HPV‐transformed premalignant lesions and about half of cervical carcinomas HPV genomes persist in an extra‐chromosomal, episomal state, whereas they are integrated into host cells chromosomes in the remaining lesions. Here we compared the methylation profile of E2BSs 1–4 of the HPV 16 URR in a series of 18 HPV16‐positive premalignant lesions and 33 invasive cervical cancers. CpGs within the E2BSs 1, 3, and 4 were higher methylated in all lesions with only episomal HPV16 genomes compared with lesions displaying single integrated copies. Samples with multiple HPV16 integrated copies displayed high methylation levels for all CpGs suggesting that the majority of multiple copies were silenced by extensive methylation. These data support the hypothesis that differential methylation of the E2BSs 1, 3 and 4 is related to the activation of viral oncogene expression in cervical lesions as long as the viral genome remains in the episomal state. Once the virus becomes integrated into host cell chromosomes these methylation patterns may be substantially altered due to complex epigenetic changes of integrated HPV genomes.
Vitamin K2 downregulates the expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma cells

Aim: Vitamin K2 exerts an antitumor activity on human hepatocellular carcinoma (HCC), however, its inhibitory mechanism has not yet been clarified. This study was designed to identify the attractive target molecule of vitamin K2 and shed some light on its effects on fibroblast growth factor receptor (FGFR)3 in HCC cells.
All Your Droid Are Belong to Us: A Survey of Current Android Attacks

In the past few years, mobile devices (smartphones, PDAs) have seen both their computational power and their data connectivity rise to a level nearly equivalent to that available on small desktop computers, while becoming ubiquitous. On the downside, these mobile devices are now an extremely attractive target for large-scale security attacks. Mobile device middleware is thus experiencing an increased focus on attempts to mitigate potential security compromises. In particular, Android incorporates by design many well-known security features such as privilege separation. The Android security model also creates several new security sensitive concepts such as Android's application permission system and the unmoderated Android market. In this paper we look to Android as a specific instance of mobile computing. We first discuss the Android security model and some potential weaknesses of the model. We then provide a taxonomy of attacks to the platform demonstrated by real attacks that in the end guarantee privileged access to the device. Where possible, we also propose mitigations for the identified vulnerabilities.
Value of fine needle aspiration biopsy in preoperative diagnosis of palpable breast lumps in resource-poor countries: a Nigerian experience.

Fine needle aspiration biopsy (FNAB) has been established as a quick simple and accurate means of diagnosing breast lesions and offers a financially attractive alternative to open surgical biopsy. This study was undertaken to assess the value of FNAB in the evaluation of breast lumps at Aminu Kano Teaching Hospital a major referral center in Kano Northern Nigeria over a 2-year period (2001-2002). A retrospective evaluation of 157 patients who had FNAB of which 93 had histological confirmation. Of the 61 histologically confirmed benign lesions 57 had been interpreted as benign cytologically while 1 and 3 cases respectively were reported as atypical and unsatisfactory. There were 32 histologically confirmed malignant cases 27 of which had been reported as malignant on FNAB 2 as suspicious and 3 as benign. The positive predictive value was 100% with a sensitivity of 90.6% and specificity of 100%. The high diagnostic accuracy obtained in this study warrants FNAB to be utilized as a first line diagnostic procedure in the evaluation of patients with palpable breast lesions in developing countries. (authors)
HIV status disclosure rate and reasons for non-disclosure among infected children and adolescents in Enugu, southeast Nigeria

Abstract Aims: To determine the rate of HIV status disclosure, caregivers’ reasons for non-disclosure, and factors influencing disclosure among a sample of HIV-infected children in Enugu, southeast Nigeria. Methods: Data were collected prospectively via a questionnaire on HIV-infected children and their caregivers who visited the pediatric HIV clinic of the University of Nigeria Teaching Hospital between July 1, 2012, and June 30, 2013. The data analysis was performed using Statistical Package for the Social Sciences version 19 software. Results: Caregivers of 107 children (age 5–16 years; mean 10.1 ± 3.2 years) were enrolled in the study. There were 53 (49.5%) boys and 54 (50.5%) girls. HIV status had been disclosed to 31 (29%) of them. The major reason for non-disclosure was the child being considered too young. Age (p < .001), age at HIV diagnosis (p < .001) and baseline CD4 count (p = .008) were seen as significant predictors of HIV disclosure. Conclusions: There is a low rate of HIV disclosure to infected children, and it was found to be lower for younger children. We recommend improving efforts for disclosure counseling to caregivers in pediatric HIV clinics.
Measurement of plasma histamine in asthma

Introduction Histamine is one of the most important mediators released from mast cells and its involvement in human hypersensitivity reactions is well established. Elevated plasma histamine concentration (H) has been reported in acute severe asthma by several authors (Bruce et ai, 1976; Simon et ai, \911; Charles el ai, 1979), Measurement of plasma H might therefore be expected to reflect degranulation of pulmonary mast cells occurring //; vivo and might offer an index of mast cell involvement in clinical or experimental asthma. However, there are several potential difficulties with the use of plasma H as an index of pulmonary mast cell discharge. The concentrations of histamine are low and, until recently, assays often lacked adequate sensitivity and specificity. At best, plasma H is an indirect measure of events in the lung because it represents spillover from much higher local tissue concentrations. The proportion of free histamine in plasma that is derived from pulmonary mast cells as opposed to other sources such as the gastric mucosa is not known and this may create a baseline error. The concept of 'free hislamine' in plasma is a diflicult one because this represents only 0 5",', of the whole blood histamine. the rest being contained in basophils. The extent to which 'plasma" H represents leakage from basophils during sampling and separation procedures is difficult to assess. Recent studies infusing histamine into normal and asthmatic subjects cast doubt on many of the values of plasma H previously reported because these levels should have, but apparently did not produce obvious pharmacodynamic efl"ects such as flushing. Improved methodology has led us to re-examine reported plasma H measurement in stable asthma, acute exacerbations, antigen-, exerciseand hyperventilationinduced wheezing, noctural asthma and propranolol, methaeholine and aspirininduced bronchoconstriction. Several authors (Bhat ('/(//. , 1976; Stevenson e/a/. 1976; Brown t'/a/. , 1980; Barnes
The Neurobiological Pathogenesis of Poststroke Depression

Poststroke depression (PSD) is an important consequence after stroke, with negative impact on stroke outcome. The pathogenesis of PSD is complicated, with some special neurobiological mechanism, which mainly involves neuroanatomical, neuron, and biochemical factors and neurogenesis which interact in complex ways. Abundant studies suggested that large lesions in critical areas such as left frontal lobe and basal ganglia or accumulation of silent cerebral lesions might interrupt the pathways of monoamines or relevant pathways of mood control, thus leading to depression. Activation of immune system after stroke produces more cytokines which increase glutamate excitotoxicity, results in more cell deaths of critical areas and enlargement of infarctions, and, together with hypercortisolism induced by stress or inflammation after stroke which could decrease intracellular serotonin transporters, might be the key biochemical change of PSD. The interaction among cytokines, glucocorticoid, and neurotrophin results in the decrease of hippocampal neurogenesis which has been proved to be important for mood control and pharmaceutical effect of selective serotonin reuptake inhibitors and might be another promising pathway to understand the pathogenesis of PSD. In order to reduce the prevalence of PSD and improve the outcome of stroke, more relevant studies are still required to clarify the pathogenesis of PSD.
Adenosine gates synaptic plasticity at hippocampal mossy fiber synapses

The release properties of synapses in the central nervous system vary greatly, not only across anatomically distinct types of synapses but also among the same class of synapse. This variation manifests itself in large part by differences in the probability of transmitter release, which affects such activity-dependent presynaptic forms of plasticity as paired-pulse facilitation and frequency facilitation. This heterogeneity in presynaptic function reflects differences in the intrinsic properties of the synaptic terminal and the activation of presynaptic neurotransmitter receptors. Here we show that the unique presynaptic properties of the hippocampal mossy fiber synapse are largely imparted onto the synapse by the continuous local action of extracellular adenosine at presynaptic A1 adenosine receptors, which maintains a low basal probability of transmitter release.
CARBON MONOXIDE IN THE COLD DEBRIS OF SUPERNOVA 1987A

We report spectroscopic and imaging observations of rotational transitions of cold CO and SiO in the ejecta of SN1987A, the first such emission detected in a supernova remnant. In addition to line luminosities for the CO J = 1–0, 2–1, 6–5, and 7–6 transitions, we present upper limits for all other transitions up to J = 13–12, collectively measured from the Atacama Large Millimeter Array, the Atacama Pathfinder EXperiment, and the Herschel Spectral and Photometric Imaging REceiver. Simple models show the lines are emitted from at least 0.01 M☉ of CO at a temperature >14 K, confined within at most 35% of a spherical volume expanding at ∼2000 km s−1. Moreover, we locate the emission within 1′′ of the central debris. These observations, along with a partial observation of SiO, confirm the presence of cold molecular gas within supernova remnants and provide insight into the physical conditions and chemical processes in the ejecta. Furthermore, we demonstrate the powerful new window into supernova ejecta offered by submillimeter observations.
Demand Deposit Contracts and the Probability of Bank Runs

Diamond and Dybvig (1983) show that while demand-deposit contracts let banks provide liquidity, they expose them to panic-based bank runs. However, their model does not provide tools to derive the probability of the bank-run equilibrium, and thus cannot determine whether banks increase welfare overall. We study a modified model in which the fundamentals determine which equilibrium occurs. This lets us compute the ex ante probability of panic-based bank runs and relate it to the contract. We find conditions under which banks increase welfare overall and construct a demand-deposit contract that trades off the benefits from liquidity against the costs of runs. Copyright 2005 by The American Finance Association.
Improved characteristics of near-band-edge and deep-level emissions from ZnO nanorod arrays by atomic-layer-deposited Al 2 O 3 and ZnO shell layers

We report on the characteristics of near-band-edge (NBE) emission and deep-level band from ZnO/Al2O3 and ZnO/ ZnO core-shell nanorod arrays (NRAs). Vertically aligned ZnO NRAs were synthesized by an aqueous chemical method, and the Al2O3 and ZnO shell layers were prepared by the highly conformal atomic layer deposition technique. Photoluminescence measurements revealed that the deep-level band was suppressed and the NBE emission was significantly enhanced after the deposition of Al2O3 and ZnO shells, which are attributed to the decrease in oxygen interstitials at the surface and the reduction in surface band bending of ZnO core, respectively. The shift of deep-level emissions from the ZnO/ZnO core-shell NRAs was observed for the first time. Owing to the presence of the ZnO shell layer, the yellow band associated with the oxygen interstitials inside the ZnO core would be prevailed over by the green luminescence, which originates from the recombination of the electrons in the conduction band with the holes trapped by the oxygen vacancies in the ZnO shell. PACS 68.65. Ac; 71.35. -y; 78.45. +h; 78.55. -m; 78.55. Et; 78.67. Hc; 81.16. Be; 85.60. Jb.