Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibition of Host Vacuolar H+-ATPase Activity by a Legionella pneumophila Effector

Legionella pneumophila is an intracellular pathogen responsible for Legionnaires' disease. This bacterium uses the Dot/Icm type IV secretion system to inject a large number of bacterial proteins into host cells to facilitate the biogenesis of a phagosome permissive for its intracellular growth. Like many highly adapted intravacuolar pathogens, L. pneumophila is able to maintain a neutral pH in the lumen of its phagosome, particularly in the early phase of infection. However, in all cases, the molecular mechanisms underlying this observation remain unknown. In this report, we describe the identification and characterization of a Legionella protein termed SidK that specifically targets host v-ATPase, the multi-subunit machinery primarily responsible for organelle acidification in eukaryotic cells. Our results indicate that after being injected into infected cells by the Dot/Icm secretion system, SidK interacts with VatA, a key component of the proton pump. Such binding leads to the inhibition of ATP hydrolysis and proton translocation. When delivered into macrophages, SidK inhibits vacuole acidification and impairs the ability of the cells to digest non-pathogenic E. coli. We also show that a domain located in the N-terminal portion of SidK is responsible for its interactions with VatA. Furthermore, expression of sidK is highly induced when bacteria begin to enter new growth cycle, correlating well with the potential temporal requirement of its activity during infection. Our results indicate that direct targeting of v-ATPase by secreted proteins constitutes a virulence strategy for L. pneumophila, a vacuolar pathogen of macrophages and amoebae.
Approximating the unsatisfiability threshold of random formulas

Let f be a random Boolean formula that is an instance of 3-SAT. We consider the problem of computing the least real number k such that if the ratio of the number of clauses over the number of variables of f strictly exceeds k , then f is almost certainly unsatisfiable. By a well-known and more or less straightforward argument, it can be shown that kF5.191. This upper bound was improved by Kamath et al. to 4.758 by first providing new improved bounds for the occupancy problem. There is strong experimental evidence that the value of k is around 4.2. In this work, we define, in terms of the random formula f, a decreasing sequence of random variables such that, if the expected value of any one of them converges to zero, then f is almost certainly unsatisfiable. By letting the expected value of the first term of the sequence converge to zero, we obtain, by simple and elementary computations, an upper bound for k equal to 4.667. From the expected value of the second term of the sequence, we get the value 4.601q . In general, by letting the U This work was performed while the first author was visiting the School of Computer Science, Carleton Ž University, and was partially supported by NSERC Natural Sciences and Engineering Research Council . of Canada , and by a grant from the University of Patras for sabbatical leaves. The second and third Ž authors were supported in part by grants from NSERC Natural Sciences and Engineering Research . Council of Canada . During the last stages of this research, the first and last authors were also partially Ž . supported by EU ESPRIT Long-Term Research Project ALCOM-IT Project No. 20244 . †An extended abstract of this paper was published in the Proceedings of the Fourth Annual European Ž Symposium on Algorithms, ESA’96, September 25]27, 1996, Barcelona, Spain Springer-Verlag, LNCS, . pp. 27]38 . That extended abstract was coauthored by the first three authors of the present paper. Correspondence to: L. M. Kirousis Q 1998 John Wiley & Sons, Inc. CCC 1042-9832r98r030253-17 253
Rethinking Procrastination: Positive Effects of "Active" Procrastination Behavior on Attitudes and Performance

Researchers and practitioners have long regarded procrastination as a self-handicapping and dysfunctional behavior. In the present study, the authors proposed that not all procrastination behaviors either are harmful or lead to negative consequences. Specifically, the authors differentiated two types of procrastinators: passive procrastinators versus active procrastinators. Passive procrastinators are procrastinators in the traditional sense. They are paralyzed by their indecision to act and fail to complete tasks on time. In contrast, active procrastinators are a "positive" type of procrastinator. They prefer to work under pressure, and they make deliberate decisions to procrastinate. The present results showed that although active procrastinators procrastinate to the same degree as passive procrastinators, they are more similar to nonprocrastinators than to passive procrastinators in terms of purposive use of time, control of time, self-efficacy belief, coping styles, and outcomes including academic performance. The present findings offer a more sophisticated understanding of procrastination behavior and indicate a need to reevaluate its implications for outcomes of individuals.
Synthesis of protocol entities specifications from service specifications in a Petri net model with registers

In general, the services of a distributed system are provided by some cooperative protocol entities. The protocol entities must exchange some data values and synchronization messages in order to ensure the temporal ordering of the events which are described in a service specification of the distributed system. It is desirable that a correct protocol entity specification for each node can be derived automatically from a given service specification. In this paper, we propose an algorithm which synthesizes a correct protocol entity specification automatically from a service specification in a Petri Net model with Registers called PNR model. In our model, parallel events and selective operations can be described naturally. The control flow of a service specification must be described as a free-choice net in order to simplify the derivation algorithm, however, many practical systems can be described in this class. In our approach, since each protocol entity specification is also described in our PNR model, we can easily understand what events can be executed in parallel at each protocol entity.
Measurements of Ocean Surface Turbulence and Wave-Turbulence Interactions (PREPRINT)

Abstract : The uppermost layers of the ocean, along with the lower atmospheric boundary layer, play a crucial role in the air-sea fluxes of momentum, heat, and mass, thereby providing important boundary conditions for both the atmosphere and the oceans that control the evolution of weather and climate. In particular, the fluxes of heat and gas rely on exchange processes through the molecular layers, which are usually located within the viscous layer, which is in turn modulated by the waves and the turbulence at the free surface. The understanding of the multiple interactions between molecular layers, viscous layers, waves, and turbulence is therefore paramount to an adequate parameterization of these fluxes. In this paper, the authors present evidence of a clear coupling between the surface waves and the surface turbulence. When averaged over time scales longer than the wave period, this coupling yields a spatial relationship between surface temperature, divergence, and vorticity fields that is consistent with spatial patterns of Langmuir turbulence. The resulting surface velocity field is hyperbolic, suggesting that significant stretching takes place in the surface layers. On time scales for which the surface wave field is resolved, they show that the surface turbulence is modulated by the waves in a manner qualitatively consistent with rapid distortion theory.
Relationships between altruistic activity and positive and negative affect among low-income older adult service providers

Self-reported altruistic activity was examined as a predictor of positive and negative affective states among a sample of 115 low-income older adults who were actively providing services to others within a federally subsidized service delivery program (Foster Grandparents and Senior Companions). Results indicated that altruistic activity was predictive of positive, but not negative affect. It was found that altruistic activity was a significant predictor of positive affect after controlling for relevant demographic variables including social support and income. The theoretical and clinical implications of this research are discussed.
Perceptual-Motor Performance and Associated Kinematics in Space

Objective: To determine whether the impairment of perceptual-motor performance often observed during the initial stages of space flight is attributable to the direct effects of microgravity on sensory-motor input-output relationships or to cognitive overload arising from the variety of stressors encountered in this environment. Background: Experimental evidence is contradictory, and the present experiment investigated the role of two variables: use of a joystick with tracking or aiming tasks and degree of arm restraint. Methods: Five well-trained astronauts performed Fitts' reciprocal aiming task on three occasions (preflight, on the International Space Station, and postflight) under the following conditions: (a) with a stylus or a joystick, (b) with the arm restrained or unrestrained, and (c) as a single task or together with auditory reaction time (RT) as a dual task. In addition to the speed and accuracy of responses, kinematic measures were derived from the joystick. Results: In space, the slope of Fitts' function increased only in the dual task condition with the joystick. RT was also slowed in the dual condition, and there was an increase in aiming and RT errors. Percentage time to peak acceleration, velocity, and deceleration were increased when the aiming task was performed alone. Conclusion: These results support the cognitive overload rather than the microgravity hypothesis and indicate that an impairment in sensory-motor performance is not a necessary concomitant of space flight. Application: Impairments in perceptual-motor performance in space can be eliminated or mitigated by appropriate training and task design.
New designer drug 1-(3-trifluoromethylphenyl) piperazine (TFMPP): gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry studies on its phase I and II metabolism and on its toxicological detection in rat urine.

Studies are described on the phase I and II metabolism and the toxicological analysis of the piperazine-derived designer drug 1-(3-trifluoromethylphenyl)piperazine (TFMPP) in rat urine using gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). The identified metabolites indicated that TFMPP was extensively metabolized, mainly by hydroxylation of the aromatic ring and by degradation of the piperazine moiety to N-(3-trifluoromethylphenyl)ethylenediamine, N-(hydroxy-3-trifluoromethylphenyl)ethylenediamine, 3-trifluoromethylaniline, and hydroxy-3-trifluoromethylaniline. Phase II reactions included glucuronidation, sulfatation and acetylation of phase I metabolites. The authors' systematic toxicological analysis (STA) procedure using full-scan GC/MS after acid hydrolysis, liquid-liquid extraction and microwave-assisted acetylation allowed the detection of TFMPP and its above-mentioned metabolites in rat urine after single administration of a dose calculated from the doses commonly taken by drug users. Assuming similar metabolism, the described STA procedure should be suitable for proof of an intake of TFMPP in human urine.
A General Model for Designing Networks of Marine Reserves

There is debate concerning the most effective conservation of marine biodiversity, especially regarding the appropriate location, size, and connectivity of marine reserves. We describe a means of establishing marine reserve networks by using optimization algorithms and multiple levels of information on biodiversity, ecological processes (spawning, recruitment, and larval connectivity), and socioeconomic factors in the Gulf of California. A network covering 40% of rocky reef habitat can fulfill many conservation goals while reducing social conflict. This quantitative approach provides a powerful tool for decision-makers tasked with siting marine reserves.
Effectiveness of phosphodiesterase-5 inhibitor therapy for portopulmonary hypertension.

BACKGROUND Portopulmonary hypertension is associated with significant morbidity and mortality. Phosphodiesterase-5 inhibitor therapy is efficacious in other causes of WHO group I pulmonary arterial hypertension. OBJECTIVE To evaluate the efficacy and safety of phosphodiesterase-5 inhibitor therapy in patients with portopulmonary hypertension. METHODS A single-centre retrospective cohort study that included patients with a diagnosis of portopulmonary hypertension was performed. The primary outcome was change in pulmonary vascular resistance after six months of phosphodiesterase-5 inhibitor therapy. A secondary evaluation investigated the effect on other hemodynamic measurements, 6 min walk distance, functional class, safety outcomes and survival. RESULTS Of 1385 patients screened, 25 patients with portopulmonary hypertension were identified, of whom 20 received a phosphodiesterase-5 inhibitor. After six months, there was a significant decrease in pulmonary vascular resistance (-236 dyn • s • cm(-5) [95% CI -343 dyn • s • cm(-5) to -130 dyn • s • cm(-5)]; P<0.001), mean pulmonary artery pressure (-8.9 mmHg [95% CI -13.7 mmHg to -4.2 mmHg]; P=0.001) and an increase in Fick cardiac output (0.9 L/min [95% CI 0.1 L/min to 1.6 L/min]; P=0.02). There was no change in 6 min walk distance. The proportion of subjects with a WHO functional class III or IV was significantly reduced at six months compared with baseline (18% versus 61%; P=0.002). Safety outcomes did not reveal any adverse events. CONCLUSIONS Phosphodiesterase-5 inhibitor therapy improved hemodynamics and functional class at six months in a cohort of patients with portopulmonary hypertension.
Application of generalized non-Schmid yield law to low-temperature plasticity in bcc transition metals

In this work, a generalized yield criterion that captures non-Schmid effects is proposed and implemented into a finite element crystal plasticity model to simulate plastic deformation of single and polycrystals. The parameters required for the constitutive formulation were calibrated to deformation experiments on single crystals. This model is used to investigate the effects of non-Schmid effects on the predictions of the stress–strain response and texture evolution in body-centered-cubic (bcc) metals. The non-Schmid contributions are required to accurately predict the stress–strain response of single crystals, and the concomitant non-associativity of the flow also increases the tendency of localization in polycrystal deformations.
Surface drawing: creating organic 3D shapes with the hand and tangible tools

Surface Drawing is a system for creating organic 3D shapes in a manner which supports the needs and interests of artists. This medium facilitates the early stages of creative design which many 3D modeling programs neglect. Much like traditional media such as line drawing and painting, Surface Drawing lets users construct shapes through repeated marking. In our case, the hand is used to mark 3D space in a semi-immersive virtual environment. The interface is completed with tangible tools to edit and manipulate models. We introduce the use of tongs to move and scale 3D shapes and demonstrate a magnet tool which is comfortably held without restricting hand motion. We evaluated our system through collaboration with artists and designers, exhibition before hundreds of users, our own extensive exploration of the medium, and an informal user study. Response was especially positive from users with an artistic background.
The influence of children's weight on the absorption of electromagnetic fields

Higher specific absorption rate (SAR) values have been found in children when exposed to RF sources. It is worth noting that mass densities of each of the tissues is always used as of that of an adult person, despite the evident difference of tissue dielectric values due to the difference of water contents in each development stage. Some researchers have reported that children may exhibit higher SAR values than the guideline levels in certain exposure conditions. Therefore, this study is conducted to find out the reason why it is the case. Numerical assessment in two child models, namely Eartha and NORMAN, has been performed when exposing to a plane wave of vertical polarization from front to back. The comparison with the muscle equivalent models has revealed that the low body weight in child models results in relatively high SAR. It is shown that around 35-40% increase in the body weight in the heterogeneous models would reduce the whole body averaged SAR values approximately by 30% and 16-19 % at 900MHz and 1900MHz, respectively.
A Systematic Review and Meta-analysis of Pylorus-preserving Versus Classical Pancreaticoduodenectomy for Surgical Treatment of Periampullary and Pancreatic Carcinoma

Objective:Comparison of effectiveness between the pylorus-preserving pancreaticoduodenectomy (“pylorus-preserving Whipple” [PPW]) and the classic Whipple (CW) procedure. Methods:A systematic literature search (Medline, Embase, Cochrane Library, Biosis, Science Citation Index, Ovid Journals) was performed to identify all eligible articles. Randomized controlled trials (RCTs) comparing PPW versus CW for periampullary and pancreatic carcinoma were eligible for inclusion. The methodologic quality of included studies was evaluated independently by 2 authors. Quantitative data on perioperative parameters (blood loss, transfusion, operation time, and length of hospital stay), mortality, morbidity, and survival were extracted from included studies for meta-analysis. Pooled estimates of overall treatment effect were calculated using a random effects model. Results:In total, 1235 abstracts were retrieved and checked for eligibility and 6 RCTs finally included. The critical appraisal revealed vast heterogeneity with respect to methodologic quality and outcome parameters. The comparison of overall in-hospital mortality (odds ratio, 0.49; 95% CI, 0.17 to 1.40; P = 0.18), morbidity (odds ratio 0.89; 95% CI, 0.48 to 1.62; P = 0.69), and survival (hazard ratio, 0.74; 95% CI, 0.52 to 1.07; P = 0.11) showed no significant difference. However, operating time (weighted mean difference, −68.26 minutes; 95% CI, −105.70 to −30.83; P = 0.0004), and intraoperative blood loss (weighted mean difference, −766 mL; 95% CI, −965.26 to −566.74; P = 0.00001) were significantly reduced in the PPW group. Conclusion:Hence, in the absence of relevant differences in mortality, morbidity, and survival, the PPW seems to be as effective as the CW. Given obvious clinical and methodological interstudy heterogeneity, efforts should be intensified in the future to perform high quality RCTs of complex surgical interventions on the basis of well defined outcome parameters.
Application of a Novel Diagnostic Rule in the Differential Diagnosis between Acute Gouty Arthritis and Septic Arthritis

Septic arthritis and gout are major diseases that should be suspected in patients with acute monoarthritis. These two diseases are clinically similar and often indistinguishable without the help of synovial fluid analysis. Recently, a novel diagnostic rule for gout without synovial fluid analysis was developed and showed relevant performances. This study aimed to determine whether this diagnostic rule could perform well in distinguishing gout from septic arthritis. The diagnostic rule comprises 7 clinical and laboratory variables, each of which is given a specified score. The probability of gout is classified into 3 groups according to the sum of the scores: high (≥ 8), intermediate (> 4 to < 8) and low probability (≤ 4). In this retrospective study, we applied this diagnostic rule to 136 patients who presented as acute monoarthritis and were subsequently diagnosed as acute gout (n = 82) and septic arthritis (n = 54) based on synovial fluid analysis. The mean sum of scores of acute gout patients was significantly higher than that of those with septic arthritis (8.6 ± 0.2 vs. 3.6 ± 0.32, P < 0.001). Patients with acute gout had significantly more 'high', and less 'low' probabilities compared to those with septic arthritis (Eta[η]: 0.776). The prevalence of acute gouty arthritis, as confirmed by the presence of monosodium crystal, was 95.5% (61/64), 57.5% (19/33), and 5.1% (2/39) in high, intermediate and low probability group, respectively. The recently introduced diagnostic rule properly discriminates acute gout from septic arthritis. It may help physicians diagnose gout in cases difficult to be differentiated from septic arthritis. Graphical Abstract
Spline Galerkin Methods for the Sherman-Lauricella Equation on Contours with Corners

Spline Galerkin approximation methods for the Sherman--Lauricella integral equation on simple closed piecewise smooth contours are studied, and necessary and sufficient conditions for their stability are obtained. It is shown that the method under consideration is stable if and only if certain operators associated with the corner points of the contour are invertible. Numerical experiments demonstrate a good convergence of the spline Galerkin methods and validate theoretical results. Moreover, it is shown that if all corners of the contour have opening angles located in interval $(0.1\pi, 1.9\pi)$, then the corresponding Galerkin method based on splines of order $0$, $1$, or $2$ is always stable. These results are in strong contrast with the behavior of the Nystrom method, which has a number of instability angles in the interval mentioned.
Learning of Social Signatures Through Imitation Game Between a Robot and a Human Partner

In this paper, a robot learns different postures by imitating several partners. We assessed the effect of the type of partners, i.e., adults, typically developing (TD) children and children with autism spectrum disorder (ASD), on robot learning during an imitation game. The experimental protocol was divided into two phases: 1) a learning phase, during which the robot produced a random posture and the partner imitated the robot; and 2) a phase in which the roles were reversed and the robot had to imitate the posture of the human partner. Robot learning was based on a sensory-motor architecture whereby neural networks (N.N.) enabled the robot to associate what it did with what it saw. Several metrics (i.e., annotation, the number of neurons needed to learn, and normalized mutual information) were used to show that the partners affected robot learning. The first result obtained was that learning was easier with adults than with both groups of children, indicating a developmental effect. Second, learning was more complex with children with ASD compared to both adults and TD children. Third, learning with the more complex partner first (i.e., children with ASD) enabled learning to be more easily generalized.