Miracle-dz/UniFAR-FTUs huggingface dataset:
The melanoma proteoglycan: restricted expression on microspikes, a specific microdomain of the cell surface

A cell surface chondroitin sulfate proteoglycan associated with human melanomas and defined by mAb's F24.47 and 48.7 has been characterized biochemically and localized by indirect immunogold electron microscopy. These antibodies recognize distinct epitopes on the intact proteoglycan. In addition, mAb 48.7 also recognizes an epitope on a 250,000-D glycoprotein and is therefore similar to antibody 9.2. 27 (described by Bumol, T.F., and R.A. Reisfeld, 1982, Proc. Natl. Acad. Sci. USA., 79:1245-1249). Furthermore, it was shown that the glycosaminoglycan chains released by alkaline borohydride treatment of the proteoglycan recognized by mAb 48.7 had a size of approximately 60,000 D. Since the intact proteoglycan was estimated to be 420,000 D, there are probably three chondroitin sulfate chains attached to the 250,000-D core glycoprotein. Furthermore, an oligosaccharide fraction containing 42% of the 3H activity (glucosamine as precursor) was isolated. Immunolocalization studies using whole-mount electron microscopy revealed that the chondroitin sulfate proteoglycan was present almost exclusively on microspikes, a microdomain of the melanoma cell surface. These processes were present as 1-2-micron structures on the upper cell surface and as longer (up to 20 micron) structures at the cell periphery. Peripheral microspikes were involved in the initial interactions between adjacent cells and formed complex footpads that made contact with the substratum. Immunogold-labeled cells were also thin sectioned and the specific localization of the chondroitin sulfate proteoglycan antigen was quantitated. The data confirmed the results of whole-mount microscopy and demonstrated a statistically significant association of the antigen with the microspike processes as compared with other areas of the cell surface. By using two different mAb's (48.7 and F24.47) that recognize epitopes on either the core glycoprotein or the intact proteoglycan, respectively, we have demonstrated that both molecules have the same restricted distribution at the cell surface. The specific localization of the antigen to microspikes at the cell surface suggests it may play a role in cell-cell contact and cell-substratum adhesion, which could be important in the metastatic process.
A comparison of pregnancy dating methods commonly used in South Africa: a prospective study.

BACKGROUND Pregnancy dating in the South African public healthcare setting is mainly based on clinical assessment. However, the accuracy of this approach is unknown. AIM To compare the accuracy of different pregnancy dating methods. METHODS We performed a prospective comparison of 2 ultrasound policies involving consecutive low-risk women in a midwife clinic in the Metro East region, Cape Town, Western Cape. Information on the last menstrual period (LMP), the 1st symphysis-to-fundal height measurement (FH) and average gestation by ultrasonographic (US) fetal biometry was recorded. Five dating methods: LMP, FH, US and their combinations, were assessed against the actual day of delivery (ADD). The main outcome measures were: (i) the days between the ADD and estimated date of delivery in pregnancies where spontaneous labour occurred and the baby had a normal birth weight; (ii) the incidence of gestational age-related outcomes; and (iii) the influence of clinical variables on dating discrepancies. RESULTS A total of 1 342 pregnancies were analysed. The accuracy of dating was similar for certain and uncertain LMP. FH was less accurate with increasing obesity. US-based dating was most accurate (for 85% of predictions within 14 days) and similarly accurate at 20 - 24 weeks and at >24 weeks. US reduced the number of assumed pre-and post-term deliveries and, in addition, was better at detecting small-for-gestational age infants (p<0.001). CONCLUSION Pregnancy dating by US, including those in more advanced pregnancies than currently permitted, is recommended since all non-ultrasound-based estimations of gestational age were considerably less accurate.
Use of laryngeal mask airway compared to endotracheal tube with positive-pressure ventilation in anesthetized swine.

OBJECTIVE To compare the ease of placement and ventilatory parameters of a laryngeal mask airway (LMA) with an endotracheal tube (ETT) in anesthetized swine during positive-pressure ventilation (PPV). STUDY DESIGN Prospective, randomized, experimental trial. ANIMALS Nine young domestic swine, weighing between 40 and 49 kg (mean 45.1 kg), being used for a separate terminal surgical study. METHODS The pigs were immobilized with tiletamine/zolazepam, 2.7-3.6 mg kg(-1), intramuscularly, followed by isoflurane in oxygen delivered by facemask. The lungs were mechanically ventilated through an ETT or an LMA, in random order, during the anesthetic period. Positive-pressure ventilation was adjusted to maintain end-tidal CO2 (Pe'CO2) between 35 and 45 mmHg, with peak inspiratory pressure (P(insp)) of 15-23 cmH2O. Buprenorphine, 0.3 mg intramuscularly, was given to each pig after instrumentation. Isoflurane vaporizer settings were adjusted to maintain a surgical plane of anesthesia. Respiratory rate (RR), tidal volume (V(T)), minute volume (V(E)), and Pe'CO2 were measured and recorded at 5-minute intervals. After the collection of 1 hour of data, the alternate airway was placed. Swine were given at least 30 minutes to stabilize and another hour of data were recorded. At the time of airway placement, the ease of placement was assessed based on time and the number of personnel required. Data were analyzed using paired Student's t-test or Wilcoxon signed rank test where appropriate. RESULTS Laryngeal mask airways were significantly easier to place than ETT. Values for V(T) and V(E) were not significantly different between treatments. Peak inspiratory pressures were higher in ETT-ventilated swine. CONCLUSIONS AND CLINICAL RELEVANCE An LMA may be used as an alternative to an ETT in mechanically-ventilated anesthetized swine. Use of an LMA may reduce time and personnel required for placement of an airway.
Enhanced Expression of Leptin and Leptin Receptor (OB-R) in Human Breast Cancer

Purpose: To evaluate leptin and leptin receptor (OB-R) expression in human breast cancer and determine whether it could be effective for the prevention and treatment of breast cancer. Experimental Design: Immunohistochemical staining using specific antibodies was used to evaluate the protein expression of leptin and OB-R in 76 invasive ductal carcinomas and 32 samples of corresponding normal mammary gland, and the relationship between the expression of OB-R and leptin and clinicopathological features was analyzed. Results: Normal mammary epithelial cells did not express a significant level of Ob-R, whereas carcinoma cells showed positive staining for OB-R in 63 (83%) cases. Both normal epithelial cells and carcinoma cells expressed a significant level of leptin. However, overexpression of leptin, as determined by staining intensity, was observed in 70 cancers (92%) but in no normal epithelium. The expression of OB-R showed a significant correlation with the level of leptin expression. Interestingly, distant metastasis was detected in 21 (34%) of 61 OB-R-positive tumors with leptin overexpression, but in none of the 15 tumors that lacked OB-R expression or leptin overexpression (P < 0.05). Consequently, patients with the former tumors showed significantly lower survival than those with the latter. Conclusions: Leptin may have a promoting effect on the carcinogenesis and metastasis of breast cancer, possibly in an autocrine manner. Functional inhibition of leptin may be effective for the prevention and treatment of breast cancer.
Sex differences in control of cutaneous blood flow.

Women are far more likely than men to suffer from Raynaud's disease. The purpose of this study was to determine whether there are gender differences in local or central control of cutaneous blood flow that could account for the increased incidence of Raynaud's disease in women. To assess cutaneous blood flow, hand blood flow (HBF), finger blood flow (FBF), or skin perfusion (SP) was measured by fluid plethysmography, mercury strain-gauge plethysmography, or laser Doppler spectroscopy, respectively, in 47 volunteers. Basal HBF in men exceeded that of women (12.1 +/- 2.0 versus 6.2 +/- 1.5 ml/100 ml/min). Likewise, FBF in men surpassed that of women (19.5 +/- 4.1 versus 7.7 +/- 1.8 ml/100 ml/min). Similarly, SP in men was greater than that of women (270 +/- 42 versus 81 +/- 16 perfusion units). However, after total body warming (to induce a thermal sympatholysis), HBF in women exceeded that of men, suggesting that the lower basal HBF in women was due to increased sympathetic outflow to the extremities. Mental stress and deep inspiration reduced HBF and SP in men. Paradoxically, both of these maneuvers increased HBF and SP in women. To determine whether these paradoxical responses in women were due to the women's elevated basal sympathetic tone, these experiments were repeated after total body cooling in men to increase sympathetic tone and after total body warming in women to reduce sympathetic tone. Total body cooling reduced HBF and SP in men. Under these conditions, mental stress and deep inspiration induced vasodilation. In women, total body warming for 10 minutes increased HBF. (ABSTRACT TRUNCATED AT 250 WORDS)
Evaluating the accuracy of the Wechsler Memory Scale-Fourth Edition (WMS-IV) logical memory embedded validity index for detecting invalid test performance

ABSTRACT Embedded performance validity tests (PVTs) allow for continuous assessment of invalid performance throughout neuropsychological test batteries. This study evaluated the utility of the Wechsler Memory Scale-Fourth Edition (WMS-IV) Logical Memory (LM) Recognition score as an embedded PVT using the Advanced Clinical Solutions (ACS) for WAIS-IV/WMS-IV Effort System. This mixed clinical sample was comprised of 97 total participants, 71 of whom were classified as valid and 26 as invalid based on three well-validated, freestanding criterion PVTs. Overall, the LM embedded PVT demonstrated poor concordance with the criterion PVTs and unacceptable psychometric properties using ACS validity base rates (42% sensitivity/79% specificity). Moreover, 15–39% of participants obtained an invalid ACS base rate despite having a normatively-intact age-corrected LM Recognition total score. Receiving operating characteristic curve analysis revealed a Recognition total score cutoff of < 61% correct improved specificity (92%) while sensitivity remained weak (31%). Thus, results indicated the LM Recognition embedded PVT is not appropriate for use from an evidence-based perspective, and that clinicians may be faced with reconciling how a normatively intact cognitive performance on the Recognition subtest could simultaneously reflect invalid performance validity.
READING HABITS IN DIGITAL ERA: A RESEARCH ON THE STUDENTS IN BORNEO UNIVERSITY

This research aims to explore the current reading habits of university students. Moreover, it aims to determine the effects of widespread use of the internet and other digital resources in reading habits and to give some possible recommendation to improve students reading habits in the digital era. The research design was descriptive survey research. The instrument of the research was questionnaire, which is based on Akarsu and Dariyemez (2014) and Chauhan and Lal (2012). The participants of the research were 320 students studying in six majors in Faculty of Teachers Training and Education at Borneo University. They were selected through the cluster random sampling. The questionnaire involved six categories, namely demographic information, frequency of items read, contents of online reading, online activities, content first clicked when online, and techniques to develop reading habits. All research data was analyzed using SPSS Statistics 22 program.DOI: doi.org/10.24071/llt.2017. 200209
Development and Validation of a New Methodology to Assess the Vineyard Water Status by On-the-Go Near Infrared Spectroscopy

Assessing water status and optimizing irrigation is of utmost importance in most winegrowing countries, as the grapevine vegetative growth, yield, and grape quality can be impaired under certain water stress situations. Conventional plant-based methods for water status monitoring are either destructive or time and labor demanding, therefore unsuited to detect the spatial variation of moisten content within a vineyard plot. In this context, this work aims at the development and comprehensive validation of a novel, non-destructive methodology to assess the vineyard water status distribution using on-the-go, contactless, near infrared (NIR) spectroscopy. Likewise, plant water status prediction models were built and intensely validated using the stem water potential (ψs) as gold standard. Predictive models were developed making use of a vast number of measurements, acquired on 15 dates with diverse environmental conditions, at two different spatial scales, on both sides of vertical shoot positioned canopies, over two consecutive seasons. Different cross-validation strategies were also tested and compared. Predictive models built from east-acquired spectra yielded the best performance indicators in both seasons, with determination coefficient of prediction (RP2) ranging from 0.68 to 0.85, and sensitivity (expressed as prediction root mean square error) between 0.131 and 0.190 MPa, regardless the spatial scale. These predictive models were implemented to map the spatial variability of the vineyard water status at two different dates, and provided useful, practical information to help delineating specific irrigation schedules. The performance and the large amount of data that this on-the-go spectral solution provides, facilitates the exploitation of this non-destructive technology to monitor and map the vineyard water status variability with high spatial and temporal resolution, in the context of precision and sustainable viticulture.
Individual strategy ratings improve the control for task difficulty effects in arithmetic problem solving paradigms

Mental arithmetic is a powerful paradigm to study problem solving using neuroimaging methods. However, the evaluation of task complexity varies significantly across neuroimaging studies. Most studies have parameterized task complexity by objective features such as the number size. Only a few studies used subjective rating procedures. In fMRI, we provided evidence that strategy self-reports control better for task complexity across arithmetic conditions than objective features (Tschentscher and Hauk, 2014). Here, we analyzed the relative predictive value of self-reported strategies and objective features for performance in addition and multiplication tasks, by using a paradigm designed for neuroimaging research. We found a superiority of strategy ratings as predictor of performance above objective features. In a Principal Component Analysis on reaction times, the first component explained over 90 percent of variance and factor loadings reflected percentages of self-reported strategies well. In multiple regression analyses on reaction times, self-reported strategies performed equally well or better than objective features, depending on the operation type. A Receiver Operating Characteristic (ROC) analysis confirmed this result. Reaction times classified task complexity better when defined by individual ratings. This suggests that participants’ strategy ratings are reliable predictors of arithmetic complexity and should be taken into account in neuroimaging research.
Penelope: The NBTI-Aware Processor

Transistors consist of lower number of atoms with every technology generation. Such atoms may be displaced due to the stress caused by high temperature, frequency and current, leading to failures. NBTI (negative bias temperature instability) is one of the most important sources of failure affecting transistors. NBTI degrades PMOS transistors whenever the voltage at the gate is negative (logic input "0"). The main consequence is a reduction in the maximum operating frequency and an increase in the minimum supply voltage of storage structures to cope for the degradation. Many PMOS transistors affected by NBTI can be found in both combinational and storage blocks since they observe a "0 " at their gates most of the time. This paper proposes and evaluates the design of Penelope, an NBTI-aware processor. We propose (i) generic strategies to mitigate degradation in both combinational and storage blocks, (ii) specific techniques to protect individual blocks by applying the global strategies, and (Hi) a metric to assess the benefits of reduced degradation and the overheads in performance and power.
Performance of BICM-SC and BICM-OFDM systems with diversity reception in non-gaussian noise and interference

In this paper, we present a general mathematical framework for performance analysis of single-carrier (SC) and orthogonal frequency division multiplexing (OFDM) systems employing popular bit-interleaved coded modulation (BICM) and multiple receive antennas. The proposed analysis is applicable to BICM systems impaired by general types of fading (including Rayleigh, Ricean, Nakagami-m, Nakagami-q, and Weibull fading) and general types of noise and interference with finite moments such as additive white Gaussian noise (AWGN), additive correlated Gaussian noise, Gaussian mixture noise, co-channel interference, narrowband interference, and ultra-wideband interference. We present an approximate upper bound for the bit error rate (BER) and an accurate closed-form approximation for the asymptotic BER at high signal-to-noise ratios for Viterbi decoding with the standard Euclidean distance branch metric. For the standard rate-1/2 convolutional code the proposed approximate upper bound and the asymptotic approximation become tight at BERs of 10-6 and 10-12, respectively. However, if the code is punctured to higher rates (e.g. 2/3 or 3/4), the asymptotic approximation also becomes tight at a BER of 10-6. Exploiting the asymptotic BER approximation we show that the diversity gain of BICM systems only depends on the free distance of the code, the type of fading, and the number of receive antennas but not on the type of noise. In contrast their coding gain strongly depends on the noise moments. Our asymptotic analysis shows that as long as the standard Euclidean distance branch metric is used for Viterbi decoding, BICM systems optimized for AWGN are also optimum for any other type of noise and interference with finite moments.
Evaluating tactile feedback and direct vs. indirect stylus input in pointing and crossing selection tasks

We present a pair of experiments that explore the effects of tactile-feedback and direct vs. indirect pen input on pointing and crossing selection tasks. While previous work has demonstrated the validity of crossing as a useful selection mechanism for pen-based computing, those experiments were conducted using an indirect input device -- one in which the pen-input and display were separated. We investigate users' performance with pointing and crossing interfaces controlled via not only an indirect input device, but also a direct input device -- one in which the pen-input and display are co-located. Results show that direct input significantly outperforms indirect input for crossing selection, but the two modalities are essentially equivalent in pointing selection. A small amount of tactile feedback is shown to be beneficial for both pointing and crossing selection, most noticeably in crossing tasks when using direct input where visual feedback is often occluded by a hand or stylus.
Hepatocellular carcinoma as extracolonic manifestation of Lynch syndrome indicates SEC63 as potential target gene in hepatocarcinogenesis

Abstract Objective. Lynch syndrome is a cancer predisposition syndrome caused by germline mutations in DNA mismatch repair (MMR) genes with microsatellite instability (MSI) as its molecular hallmark. Hepatocellular carcinoma (HCC) has not been considered part of the tumor spectrum. The aim was to provide a detailed molecular characterization of an HCC associated with Lynch Syndrome (Muir-Torre variant). Materials and methods. HCC samples were analyzed for MSI, MMR protein expression and coding microsatellite instability (cMSI). Since cMSI also affected SEC63 coding for an endoplasmic reticulum membrane protein with implications for intracellular protein translocation, its impact on hepatocyte growth control was assessed in an established short-term model. Recombinant inbred mouse lines (BXD) showing different basal SEC63 expression levels were treated with the chemocarcinogen diethylnitrosamine (DEN) intraperitoneally. Proliferation and apoptosis of hepatocytes were determined after 48 h using Ki67 and TUNEL assays. Results. The HCC was high-grade microsatellite unstable with loss of MSH2 expression. cMSI was detected in four genes (ASTE1, SEC63, TAF1B, TGFBR2). However, only TGFBR2 is known to be involved in hepatocarcinogenesis. When investigating the impact of SEC63 expression on hepatocyte growth control in the murine model, low hepatic expression correlated significantly (p < 0.05) with a decrease in apoptosis and increased proliferative activity. Conclusions. For the first time, an HCC with characteristic molecular features of association with Lynch syndrome is described. The pro-carcinogenic growth behavior of hepatocytes with low SEC63 expression in the murine model indicates a potential role for SEC63 in hepatocarcinogenesis in general, but this needs further functional validation.
Parameter Estimation for Nonlinear Biological System Model Based on Global Sensitivity Analysis

Mathematical models of cell signal transduction networks are normally highly nonlinear and complex, which consist of a large number of reaction species and kinetics parameters. An important problem of systems biology is to develop mathematical models of nonlinear biological systems, and to effectively estimate the unknown parameters. In this work, a novel algorithm to estimate parameters based on global sensitivity analysis is proposed, and extended Kalman filter is applied to estimate the unknown sensitive parameters of signaling transduction networks model. Taking an IκBα-NF-κB signaling pathway model as an example, simulation analysis demonstrates that the algorithm can well estimate the unknown parameters under the disturbs of the noise, and it provides an efficient method for solving the parameters' uncertainty effects of biological pathways.