Miracle-dz/UniFAR-FTUs huggingface dataset:
Polycomb Group Proteins RING1A and RING1B Regulate the Vegetative Phase Transition in Arabidopsis

Polycomb group (PcG) protein-mediated gene silencing is a major regulatory mechanism in higher eukaryotes that affects gene expression at the transcriptional level. Here, we report that two conserved homologous PcG proteins, RING1A and RING1B (RING1A/B), are required for global H2A monoubiquitination (H2Aub) in Arabidopsis. The mutation of RING1A/B increased the expression of members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) gene family and caused an early vegetative phase transition. The early vegetative phase transition observed in ring1a ring1b double mutant plants was dependent on an SPL family gene, and the H2Aub status of the chromatin at SPL locus was dependent on RING1A/B. Moreover, mutation in RING1A/B affected the miRNA156a-mediated vegetative phase transition, and RING1A/B and the AGO7-miR390-TAS3 pathway were found to additively regulate this transition in Arabidopsis. Together, our results demonstrate that RING1A/B regulates the vegetative phase transition in Arabidopsis through the repression of SPL family genes.
Respiratory functional status after conventional and minimally invasive aortic valve replacement surgery – a propensity score analysis

Introduction Reports describing respiratory function of patients after conventional or minimally invasive cardiac surgery are infrequent. Aim To compare pulmonary functional status after conventional (AVR) and after minimally invasive, through right anterior minithoracotomy, aortic valve replacement (RT-AVR). Material and methods This was an observational analysis of 212 patients scheduled for RT-AVR and 212 for AVR between January 2011 and December 2014 selected using propensity score matching. Respiratory function based on spirometry examinations is presented. Results Hospital mortality was 1.4% in RT-AVR and 1.9% in AVR (p = 0.777). Predicted mortality (EuroSCORE II) was 3.2 ±1.1% in RT-AVR and 3.1 ±1.6% in AVR (p = 0.298). Mechanical ventilation time in intensive care unit (ICU) was 7.3 ±3.9 h for RT-AVR and 9.6 ±5.5 h for AVR patients (p < 0.001). Seven days and 1 month after surgery, the reduction of spirometry functional tests was greater in the AVR group than in the RT-AVR group (p < 0.001). Three months after surgery, all spirometry parameters were still reduced and had not returned to preoperative values in both RT-AVR and AVR groups. However, the difference in spirometry values was no longer statistically significant between RT-AVR and AVR groups. Presence of chronic obstructive pulmonary disease and conventional AVR surgical technique were associated with lower values of spirometry parameters after surgery in linear median regression. Conclusions Respiratory function based on spirometry examinations was less impaired after minimally invasive RT-AVR surgery in comparison to conventional AVR surgery through median sternotomy.
Cisplatin-Induced DNA Damage Activates Replication Checkpoint Signaling Components that Differentially Affect Tumor Cell Survival

Cisplatin and other platinating agents are some of the most widely used chemotherapy agents. These drugs exert their antiproliferative effects by creating intrastrand and interstrand DNA cross-links, which block DNA replication. The cross-links mobilize signaling and repair pathways, including the Rad9-Hus1-Rad1-ATR-Chk1 pathway, a pathway that helps tumor cells survive the DNA damage inflicted by many chemotherapy agents. Here we show that Rad9 and ATR play critical roles in helping tumor cells survive cisplatin treatment. However, depleting Chk1 with small interfering RNA or inhibiting Chk1 with 3-(carbamoylamino)-5-(3-fluorophenyl)-N-(3-piperidyl)thiophene-2-carboxamide (AZD7762) did not sensitize these cells to cisplatin, oxaliplatin, or carboplatin. Moreover, when Rad18, Rad51, BRCA1, BRCA2, or FancD2 was disabled, Chk1 depletion did not further sensitize the cells to cisplatin. In fact, Chk1 depletion reversed the sensitivity seen when Rad18 was disabled. Collectively, these studies suggest that the pharmacological manipulation of Chk1 may not be an effective strategy to sensitize tumors to platinating agents.
Pilot-Scale Production and Characterization of Paramyosin, a Vaccine Candidate for Schistosomiasis Japonica

ABSTRACT Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine development. Paramyosin, a myofibrillar protein found only in invertebrates, has been widely studied as a vaccine candidate for both Schistosoma mansoni and Schistosoma japonicum. Recently, we demonstrated that Th2-biased immune responses to paramyosin are associated with resistance to reinfection with S. japonicum in humans; however, challenges in the pilot-scale production of schistosome paramyosin have hampered further studies of this promising vaccine candidate. Here we report a method for the pilot-scale expression and purification of recombinant S. japonicum paramyosin (rSj97). rSj97 was extracted from Escherichia coli inclusion bodies and purified with sequential anion-exchange, hydroxyapatite, and size exclusion chromatography. The purified rSj97 was >95% pure as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis and was free of significant endotoxin contamination. We demonstrate that, like native paramyosin, rSj97 adopts an alpha-helical coiled-coil tertiary structure and binds immunoglobulin and collagen. Naïve mice infected with S. japonicum produce anti-rSj97 immunoglobulin G (IgG) antibodies as early as 4 weeks postinfection, while sera collected from S. japonicum-infected individuals contain anti-rSj97 IgE antibodies. Our method for pilot-scale production of recombinant full-length paramyosin will facilitate preclinical evaluation of paramyosin as a vaccine for schistosomiasis.
Inferring multilateral peering

The AS topology incompleteness problem is derived from difficulties in the discovery of p2p links, and is amplified by the increasing popularity of Internet eXchange Points (IXPs) to support peering interconnection. We describe, implement, and validate a method for discovering currently invisible IXP peering links by mining BGP communities used by IXP route servers to implement multilateral peering (MLP), including communities that signal the intent to restrict announcements to a subset of participants at a given IXP. Using route server data juxtaposed with a mapping of BGP community values, we can infer 206K p2p links from 13 large European IXPs, four times more p2p links than what is directly observable in public BGP data. The advantages of the proposed technique are threefold. First, it utilizes existing BGP data sources and does not require the deployment of additional vantage points nor the acquisition of private data. Second, it requires only a few active queries, facilitating repeatability of the measurements. Finally, it offers a new source of data regarding the dense establishment of MLP at IXPs.
A comparison of phase space reconstruction and spectral coherence approaches for diagnostics of bar and end-ring connector breakage faults in polyphase induction motors using current waveforms

Two signal (waveform) analysis approaches are investigated in this paper for motor drive fault identification-one linear and the other nonlinear. Twenty-one different motor-drive operating conditions including healthy, 1 through 10 broken bars, and 1 through 10 broken end-ring connectors are investigated. Highly accurate numerical simulations of current waveforms for the various operating conditions are generated using the time stepping coupled finite element-state space method for a 208-V, 60-Hz, 2-pole, 1.2-hp, squirrel cage 3-phase induction motor. The linear signal analysis method is based on spectral coherence, whereas the nonlinear signal analysis method is based on stochastic models of reconstructed phase spaces. Conclusions resulting from the comparisons of these two methods are drawn.
The Late Triassic pseudosuchian Revueltosaurus callenderi and its implications for the diversity of early ornithischian dinosaurs

A new discovery of skeletons of Revueltosaurus callenderi from the Upper Triassic Chinle Formation of Petrified Forest National Park, Arizona clearly shows that Revueltosaurus is not an ornithischian dinosaur as previously supposed. Features such as the presence of a postfrontal, crocodile-normal ankle and paramedian osteoderms with anterior bars place R. callenderi within the Pseudosuchia, closer to crocodylomorphs than to dinosaurs. Therefore, dental characters previously used to place Revueltosaurus within the Ornithischia evolved convergently among other archosaur taxa, and cannot be used to diagnose ornithischian dinosaur teeth. As a result, all other putative North American Late Triassic ornithischians, which are all based exclusively on teeth, are cast into doubt. The only reasonably well-confirmed Late Triassic ornithischians worldwide are Pisanosaurus mertii and an unnamed heterodontosaurid from Argentina. This considerably changes the understanding of early dinosaur diversity, distribution and evolution in the Late Triassic.
Perceptions of asthma by adolescents at home.

OBJECTIVES To test symptom perception in asthma under natural circumstances and to establish relationships between changes in airway obstruction as indicated by wheeze, dyspnea, general sensations, and emotional state. DESIGN Continuous in vivo monitoring. METHOD Symptom perception was tested in 30 adolescents with severe, unstable asthma. They were continuously monitored in their homes for 72 h. Symptom perception was defined as the relation between self-reported dyspnea and airway obstruction as evident from audible wheeze. Tracheal sounds were continuously recorded with wireless telemetry for wheeze assessment. Dyspnea was assessed four times per day on a Likert-type 10-point scale, as well as four times randomly after pager remote command. The subjects kept records of use of medication, daily activities, general symptoms, and mood state in a diary. RESULTS There were nine subjects with one or two wheeze episodes, another three subjects with three or four episodes, and one subject with almost continuous wheeze. The presence of wheeze in general related significantly to a rise (from individual baseline) in dyspnea of > 2.5 scale points. Acute wheeze was the best predictor of a rise in dyspnea, but prolonged wheeze correlated significantly with negative mood and general symptoms. CONCLUSION Patients with prolonged airway obstruction perceived symptoms less well and were more vulnerable to negative effects of asthma than patients with acute onset airway obstruction.
Adaptive Sliding-Mode Control of Piezoelectric Actuators

In this paper, we consider the adaptive control problem for piezoelectric actuators with nonlinear uncertainties. The controller consists of two parts: sliding mode and adaptive component. The sliding-mode part rejects dominant uncertainties, while the adaptive component is continuously refined based on just prevailing input and output signals. The stability of the designed control system can be guaranteed by a rigorous proof. Simulation and real-time experimental results are provided to verify the effectiveness of the proposed scheme.
Approximate maximum margin algorithms with rules controlled by the number of mistakes

We present a family of incremental Perceptron-like algorithms (PLAs) with margin in which both the "effective" learning rate, defined as the ratio of the learning rate to the length of the weight vector, and the misclassification condition are entirely controlled by rules involving (powers of) the number of mistakes. We examine the convergence of such algorithms in a finite number of steps and show that under some rather mild conditions there exists a limit of the parameters involved in which convergence leads to classification with maximum margin. An experimental comparison of algorithms belonging to this family with other large margin PLAs and decomposition SVMs is also presented.
Consumer Acceptance of Online Agent Advice: Extremity and Positivity Effects

Consumers often search the Internet for agent advice when making decisions about products and services. Existing research on this topic suggests that past opinion agreement between the consumer and an agent is an important cue in consumers’ acceptance of current agent advice. In this article, we report the results of two experiments which show that different types of past agreements can have different effects on the acceptance of current agent advice. In Study 1, we show that in addition to the overall agreement rate, consumers pay special attention to extreme opinion agreement when assessing agent diagnosticity (i.e., extremity effect). In Study 2 we show that positive extreme agreement is more influential than negative extreme agreement when advice valence is positive, but the converse does not hold when advice valence is negative (i.e., positivity effect). We conclude by identifying promising avenues for future research and discuss implications of the results for marketers in areas such as design of intelligent online recommendation systems and word-of-mouth management on the Internet.
Living Up to Expectations: How Job Training Made Women Better Off and Men Worse Off

We study the interaction between job and soft skills training on expectations and labor market outcomes in the context of a youth training program in the Dominican Republic. Program applicants were randomly assigned to one of 3 modalities: a full treatment consisting of hard and soft skills training plus an internship, a partial treatment consisting of soft skills training plus an internship, or a control group. We find strong and lasting effects of the program on personal skills acquisition and expectations, but these results are markedly different for young men and young women. Shortly after completing the program, both male and female participants report increased expectations for improved employment and livelihoods. This result is reversed for male participants in the long run, a result that can be attributed to the program’s negative short-run effects on labor market outcomes for males. While these effects seem to dissipate in the long run, employed men are substantially more likely to be searching for another job. On the other hand, women experience improved labor market outcomes in the short run and exhibit substantially higher levels of personal skills in the long run. These results translate into women being more optimistic, having higher self-esteem and lower fertility in the long run. Our results suggest that job-training programs of this type can be transformative – for women, life skills mattered and made a difference, but they can also have a downside if, like in this case for men, training creates expectations that are not met.
The Language–Number Interface in the Brain: A Complex Parametric Study of Quantifiers and Quantities

The neural bases for numerosity and language are of perennial interest. In monkeys, neural separation of numerical Estimation and numerical Comparison has been demonstrated. As linguistic and numerical knowledge can only be compared in humans, we used a new fMRI paradigm in an attempt to dissociate Estimation from Comparison, and at the same time uncover the neural relation between numerosity and language. We used complex stimuli: images depicting a proportion between quantities of blue and yellow circles were coupled with sentences containing quantifiers that described them (e.g., “most/few of the circles are yellow”). Participants verified sentences against images. Both Estimation and Comparison recruited adjacent, partially overlapping bi-hemispheric fronto-parietal regions. Additional semantic analysis of positive vs. negative quantifiers involving the interpretation of quantity and numerosity specifically recruited left area 45. The anatomical proximity between numerosity regions and those involved in semantic analysis points to subtle links between the number system and language. Results fortify the homology of Estimation and Comparison between humans and monkeys.
Augmentation of a Loosened Sacral Pedicle Screw With Percutaneous Polymethylmethacrylate Injection

Study Design Case report describing augmentation of a loosened sacral screw with percutaneous injection of polymethylmethacrylate. Objective To highlight the advantages of percutaneous injection of polymethylmethacrylate in treatment of loosened pedicle screws. Setting Turkey. Summary of Background Data Lumbosacral instrumentation with pedicle screws is a commonly used procedure for correcting deformity and stabilizing the spine until bony fusion occurs. Loosening of the screws is a complication of this procedure and is seen more frequently in multilevel instrumentation, particularly in those instrumentations using sacral screws. Method We present the case of a woman who had had L3-L4-L5-S1 instrumentation with pedicle screws who developed severe low back pain radiating to the left hip 2 months after the operation. Radiologic and clinical examination revealed that the left pedicle screw of the sacrum had loosened, and revision surgery was planned. The left sacral screw was augmented with percutaneous injection of polymethylmethacrylate. Results The patient was free of pain after the operation. Conclusions We suggest that percutaneous polymethylmethacrylate injection around a loosened screw may be an alternative to major open revision surgery in cases of sacral screw failure in multisegmental instrumentation.
Mucosal Adjuvanticity and Immunogenicity of LTR72, a Novel Mutant of Escherichia coli Heat-labile Enterotoxin with Partial Knockout of ADP-ribosyltransferase Activity

Heat-labile Escherichia coli enterotoxin (LT) has the innate property of being a strong mucosal immunogen and adjuvant. In the attempt to reduce toxicity and maintain the useful immunological properties, several LT mutants have been produced. Some of these are promising mucosal adjuvants. However, so far, only those that were still toxic maintained full adjuvanticity. In this paper we describe a novel LT mutant with greatly reduced toxicity that maintains most of the adjuvanticity. The new mutant (LTR72), that contains a substitution Ala → Arg in position 72 of the A subunit, showed only 0.6% of the LT enzymatic activity, was 100,000-fold less toxic than wild-type LT in Y1 cells in vitro, and was at least 20 times less effective than wild-type LT in the rabbit ileal loop assay in vivo. At a dose of 1 μg, LTR72 exhibited a mucosal adjuvanticity, similar to that observed with wild-type LT, better than that induced by the nontoxic, enzymatically inactive LTK63 mutant, and much greater than that of the recombinant B subunit. This trend was consistent for both the amounts and kinetics of the antibody induced, and priming of antigen-specific T lymphocytes. The data suggest that the innate high adjuvanticity of LT derives from the independent contribution of the nontoxic AB complex and the enzymatic activity. LTR72 optimizes the use of both properties: the enzymatic activity for which traces are enough, and the nontoxic AB complex, the effect of which is dose dependent. In fact, in dose–response experiments in mice, 20 μg of LTR72 were a stronger mucosal adjuvant than wild-type LT. This suggests that LTR72 may be an excellent candidate to be tested in clinical trials.
Psychometric properties of Social Rhythm Metric in regular shift employees.

OBJECTIVE To analyze the psychometric properties of Brazilian version of Social Rhythm Metric-17 and to present the score algorithm in regular shift employees. METHOD This is a cross-sectional study. The Social Rhythm Metric assess the regularity of daily life associated to changes in biological rhythms. The scale was applied, during nine days, to 145 employees, 27.6 % male and 72.4% female, ages between 18 and 60, mean = 36.1 (sd = 9.87). Mean values were compared using the Student's t-test for independent samples and ANOVA. Factor analysis was performed using principal component analysis; the reliability analysis, through Spearman's correlation. RESULTS The Student t test didn't reveal any significant difference between sex in IRA (t = 0.60; p = 0.55) or ETA (t = 0.67; p = 0.95). The factor analysis showed three components, the first was associated to survival related activities; the second, related to pleasure and work and the third was related to no essential survive activities. DISCUSSION This version of the Social Rhythm Metric-17 instrument allows to assess an important human zeitgeber, contributing for other studies which can to evaluate the determinant role of social rhythm. CONCLUSION The Brazilian version of social rhythm metric 17 presents validity of content and good test-retest reliability.
The 2010 California Research at the Nexus of Air Quality and Climate Change (CalNex) field study

The California Research at the Nexus of Air Quality and Climate Change (CalNex) field study was conducted throughout California in May, June, and July of 2010. The study was organized to address issues simultaneously relevant to atmospheric pollution and climate change, including (1) emission inventory assessment, (2) atmospheric transport and dispersion, (3) atmospheric chemical processing, and (4) cloud‐aerosol interactions and aerosol radiative effects. Measurements from networks of ground sites, a research ship, tall towers, balloon‐borne ozonesondes, multiple aircraft, and satellites provided in situ and remotely sensed data on trace pollutant and greenhouse gas concentrations, aerosol chemical composition and microphysical properties, cloud microphysics, and meteorological parameters. This overview report provides operational information for the variety of sites, platforms, and measurements, their joint deployment strategy, and summarizes findings that have resulted from the collaborative analyses of the CalNex field study. Climate‐relevant findings from CalNex include that leakage from natural gas infrastructure may account for the excess of observed methane over emission estimates in Los Angeles. Air‐quality relevant findings include the following: mobile fleet VOC significantly declines, and NOx emissions continue to have an impact on ozone in the Los Angeles basin; the relative contributions of diesel and gasoline emission to secondary organic aerosol are not fully understood; and nighttime NO3 chemistry contributes significantly to secondary organic aerosol mass in the San Joaquin Valley. Findings simultaneously relevant to climate and air quality include the following: marine vessel emissions changes due to fuel sulfur and speed controls result in a net warming effect but have substantial positive impacts on local air quality.