Miracle-dz/UniFAR-FTUs huggingface dataset:
Piezoelectric Drop-on-Demand Inkjet Printing of Rat Fibroblast Cells: Survivability Study and Pattern Printing

A novel piezoelectric, drop-on-demand (DOD) inkjet system has been developed and used to print L929 rat fibroblast cells. We investigate the survivability of the cells subjected to the large stresses during the printing process. These stresses are varied by changing the diameter of the orifice (36 to 119 microns) through which the cells are dispensed, as well as changing the electrical pulse used to drive the piezoelectric element. It is shown that for the smallest 36 microns diameter orifice, cell survival rates fall from 95% to approximately 76% when the ejection velocity is increased from 2 to 16 m/s. This decrease in survival rates is less significant when the larger orifice diameters of 81 microns and 119 microns are used. Analysis shows that there is a clear inverse relationship between cell survival rates and the mean shear rates during drop formation. By using the same printing set-up, fibroblast cells are printed onto alginate and collagen into patterns. Printed cells are cultured over a period of days to verify their long-term viability. Fibroblasts printed onto the collagen are found to successfully adhere, spread and proliferate, subsequently forming a denser patterns after 5 days in culture. Cell agglomeration is found to affect the printing performance, especially for the printhead with the smallest orifice, leading to frequent clogging of the nozzle. We also study the number of cells in each droplet, when printed under optimal conditions. The probability density of this number follows a binomial distribution, which consistent with a uniform distribution of cells in the medium and within the printhead.
Computer-Based and Online Therapy for Depression and Anxiety in Children and Adolescents.

OBJECTIVE The purpose of this study was to provide an overview of computer-based and online therapies (e-therapy) to treat children and adolescents with depression and/or anxiety, and to outline programs that are evidence based or currently being researched. METHODS We began by defining the topic and highlighting the issues at the forefront of the field. We identified computer and Internet-based interventions designed to prevent or treat depression or anxiety that were tested with children and young people <18 years of age (or inclusive of this age range together with emerging adults). We included randomized controlled trials (RCTs). We summarized available relevant systematic reviews. RESULTS There is an increasing body of evidence that supports the use of computers and the Internet in the provision of interventions for depression and anxiety in children and adolescents. A number of programs have been shown to be effective in well-designed RCTs. Replication and long-term follow-up studies are needed to confirm results. CONCLUSIONS There are now a range of effective computerized interventions for young people with depression and anxiety. This is likely to impact positively on attempts to make psychological therapies widely available to children and young people. We expect to see increased program sophistication and a proliferation of programs in the coming years. Research efforts, when developing programs, need to align with technological advances to maximize appeal. Implementation research is needed to determine the optimal modes of delivery and effectiveness of e-therapies in clinical practice. Given the large number of unproven program on the Internet, ensuring that there is clear information for patients about evidence for individual programs is likely to present a challenge.
Anti-drug antibodies in Colombian patients with rheumatoid arthritis treated with Enbrel vs Etanar – Preliminary report

Abstract The present study was undertaken to detect antibodies against etanercept (ETN) in a group of Colombian patients with rheumatoid arthritis (RA) and being treated with Enbrel® vs. Etanar®. From these patients with RA, clinical and laboratory data were collected and serum taken for anti-drug antibody (ADAb) analysis. Samples from 32 patients (16 who had been treated with Enbrel® and 16 with Etanar®) were analyzed. Positive sera for ADAb were found in six of the 32 subjects (18.7%); five (31.2%) in the Enbrel® group and one (6.25%) in the Etanar® group. Patients under treatment with Enbrel® registered a longer disease duration than patients being treated with Etanar® (15.4 years vs. 10.98 years, p = 0.175) as well as a longer average treatment with the drug (45.7 vs. 23.9 months, p = 0.052). The percentage of patients with disease activity defined as a disease activity score by C-reactive protein (DAS28-CRP) scores ≥2.3 was higher in those patients with positive sera in the enzyme-linked immunosorbent assay (ELISA) (66.7%) than in those with negative sera (34.6%). A logistic regression test revealed that the higher the DAS28-CRP value, the higher the ELISA absorbance value. The results showed evidence of greater frequency of ADAb in patients treated with ETN than has been reported to date. Greater disease activity was seen in those patients in whose serum ADAb had been detected. Significant differences were found between the positive ELISA for the group of patients treated with Enbrel® compared to those treated with Etanar®. Some of the factors that could explain this difference are the length of the treatment time with the drug, the commercial ELISA kit used to detect ADAb, or the immunogenicity itself of each product.
A note on compact solvmanifolds with Kahler structures

In this note we show that a compact solvmanifold admits a Kstructure if and only if it is a finite quotient of a complex torus which has a structure of a com- plex torus bundle over a complex torus. We can show in particular that a compact solvmanifold of completely solvable type has a Kstructure if and only if it is a complex torus, which is known as the Benson-Gordon's conje cture.
The Head Tracks and Gaze Predicts: How the World’s Best Batters Hit a Ball

Hitters in fast ball-sports do not align their gaze with the ball throughout ball-flight; rather, they use predictive eye movement strategies that contribute towards their level of interceptive skill. Existing studies claim that (i) baseball and cricket batters cannot track the ball because it moves too quickly to be tracked by the eyes, and that consequently (ii) batters do not – and possibly cannot – watch the ball at the moment they hit it. However, to date no studies have examined the gaze of truly elite batters. We examined the eye and head movements of two of the world’s best cricket batters and found both claims do not apply to these batters. Remarkably, the batters coupled the rotation of their head to the movement of the ball, ensuring the ball remained in a consistent direction relative to their head. To this end, the ball could be followed if the batters simply moved their head and kept their eyes still. Instead of doing so, we show the elite batters used distinctive eye movement strategies, usually relying on two predictive saccades to anticipate (i) the location of ball-bounce, and (ii) the location of bat-ball contact, ensuring they could direct their gaze towards the ball as they hit it. These specific head and eye movement strategies play important functional roles in contributing towards interceptive expertise.
Interactions between intestinal pathogens, enteropathy and malnutrition in developing countries

Purpose of review This review focuses on recent data highlighting the interactions between intestinal pathogens, enteropathy and malnutrition in developing countries, which drive morbidity and mortality and hinder the long-term developmental potential of children. Recent findings Diarrhoea remains the second commonest cause of death in children below 5 years, and malnutrition underlies 45% of all child deaths. Even in the absence of diarrhoea, subclinical pathogen carriage and enteropathy are almost universal in developing countries. Here, we review recent studies addressing the causes and consequences of diarrhoea; emerging data on environmental influences that govern postnatal development of the gut and microbiota; current concepts of environmental enteric dysfunction; and recent intervention trials in the field. We highlight the interactions between these processes, whereby intestinal pathogens drive a cycle of gut damage, malabsorption, chronic inflammation and failed mucosal regeneration, leading to malnutrition and susceptibility to further enteric infections. Summary Efforts to improve child survival and long-term developmental potential need to address the overlapping and interacting effects of diarrhoea, enteropathy and malnutrition. Recent insights from human and animal studies suggest potential targets for intervention.
A case of deep infection after instrumentation in dorsal spinal surgery: the management with antibiotics and negative wound pressure without removal of fixation

Until today the role of spinal instrumentation in the presence of a wound infection has been widely discussed and recently many authors leave the hardware in place with appropriate antibiotic therapy. This is a case of a 65-year-old woman suffering from degenerative scoliosis and osteoporotic multiple vertebral collapses treated with posterior dorsolumbar stabilisation with screws and rods. Four months later, skin necrosis and infection appeared in the cranial wound with exposure of the rods. A surgical procedure of debridement of the infected tissue and package with a myocutaneous trapezius muscle flap was performed. One week after surgery, negative pressure wound therapy was started on the residual skin defect. The wound healed after 2 months. The aim of this case report is to focus on the utility of this method even in the case of hardware exposure and infection. This may help avoid removing instrumentation and creating instability.
DENSITY: software for analysing capture-recapture data from passive detector arrays

DENSITY: software for analysing capture–recapture data from passive detector arrays. — A general computer–intensive method is described for fitting spatial detection functions to capture–recapture data from arrays of passive detectors such as live traps and mist nets. The method is used to estimate the population density of 10 species of breeding birds sampled by mist–netting in deciduous forest at Patuxent Research Refuge, Laurel, Maryland, U.S.A. , from 1961 to 1972. Total density (9.9 ± 0.6 ha –1 mean ± SE) appeared to decline over time (slope –0.41 ± 0.15 ha –1 y –1 ). The mean precision of annual estimates for all 10 species pooled was acceptable ( = 14%). Spatial analysis of closed–population capture– recapture data highlighted deficiencies in non–spatial methodologies. For example, effective trapping area cannot be assumed constant when detection probability is variable. Simulation may be used to evaluate alternative designs for mist net arrays where density estimation is a study goal.
Evidence that autistic traits show the same etiology in the general population and at the quantitative extremes (5%, 2.5%, and 1%).

CONTEXT Genetic factors play an important role in the etiology of both autism spectrum disorders and autistic traits. However, little is known about the etiologic consistency of autistic traits across levels of severity. OBJECTIVE To compare the etiology of typical variation in autistic traits with extreme scoring groups (including top 1%) that mimicked the prevalence of diagnosed autism spectrum disorders in the largest twin study of autistic traits to date. DESIGN Twin study using phenotypic analysis and genetic model-fitting in the total sample and extreme scoring groups (top 5%, 2.5%, and 1%). SETTING A nationally representative twin sample from the general population of England. PARTICIPANTS The families of 5968 pairs aged 12 years old in the Twins' Early Development Study. Main Outcome Measure Autistic traits as assessed by the Childhood Autism Spectrum Test. RESULTS Moderate to high heritability was found for autistic traits in the general population (53% for females and 72% for males). High heritability was found in extreme-scoring groups. There were no differences in heritability among extreme groups or between the extreme groups and the general population. A continuous liability shift toward autistic trait affectedness was seen in the cotwins of individuals scoring in the top 1%, suggesting shared etiology between extreme scores and normal variation. CONCLUSION This evidence of similar etiology across normal variation and the extremes has implications for molecular genetic models of autism spectrum disorders and for conceptualizing autism spectrum disorders as the quantitative extreme of a neurodevelopmental continuum.
A sensitized mutagenesis screen in Factor V Leiden mice identifies novel thrombosis suppressor loci

Factor V Leiden (F5L) is a common genetic risk factor for venous thromboembolism in humans. We conducted a sensitized ENU mutagenesis screen for dominant thrombosuppressor genes based on perinatal lethal thrombosis in mice homozygous for F5L (F5L/L) and haploinsufficient for tissue factor pathway inhibitor (Tfpi+/−). F8 deficiency enhanced survival of F5L/L Tfpi+/− mice, demonstrating that F5L/L Tfpi+/− lethality is genetically suppressible. ENU-mutagenized F5L/L males and F5L/+ Tfpi+/− females were crossed to generate 6,729 progeny, with 98 F5L/L Tfpi+/− offspring surviving until weaning. Sixteen lines exhibited transmission of a putative thrombosuppressor to subsequent generations, with these lines referred to as MF5L (Modifier of Factor 5 Leiden) 1-16. Linkage analysis in MF5L6 identified a chromosome 3 locus containing the tissue factor gene (F3). Though no ENU-induced F3 mutation was identified, haploinsufficiency for F3 (F3+/−) suppressed F5L/L Tfpi+/− lethality. Whole exome sequencing in MF5L12 identified an Actr2 gene point mutation (p.R258G) as the sole candidate. Inheritance of this variant is associated with suppression of F5L/L Tfpi+/− lethality (p=1.7x10−6), suggesting that Actr2p.R258G is thrombosuppressive. CRISPR/Cas9 experiments to generate an independent Actr2 knockin/knockout demonstrated that Actr2 haploinsufficiency is lethal, supporting a hypomorphic or gain of function mechanism of action for Actr2p.R258G. Our findings identify F8 and the Tfpi/F3 axis as key regulators in determining thrombosis balance in the setting of F5L and also suggest a novel role for Actr2 in this process. Significance Statement Venous thromboembolism (VTE) is a common disease characterized by the formation of inappropriate blood clots. Inheritance of specific genetic variants, such as the Factor V Leiden polymorphism, increases VTE susceptibility. However, only ~10% of people inheriting Factor V Leiden develop VTE, suggesting the involvement of other genes that are currently unknown. By inducing random genetic mutations into mice with a genetic predisposition to VTE, we identified two genomic regions that reduce VTE susceptibility. The first includes the gene for blood coagulation Factor 3 and its role was confirmed by analyzing mice with an independent mutation in this gene. The second contains a mutation in the Actr2 gene. These findings identify critical genes for the regulation of blood clotting risk.
Ethnicity- and Sex-Based Discrimination and the Maintenance of Self-Esteem

The psychological underpinnings of labor market discrimination were investigated by having participants from Israel, the West Bank and Germany (N = 205) act as employers in a stylized employment task in which they ranked, set wages, and imposed a minimum effort level on applicants. State self-esteem was measured before and after the employment task, in which applicant ethnicity and sex were salient. The applicants were real people and all behavior was monetarily incentivized. Supporting the full self-esteem hypothesis of the social identity approach, low self-esteem in women was associated with assigning higher wages to women than to men, and such behavior was related to the maintenance of self-esteem. The narrower hypothesis that successful intergroup discrimination serves to protect self-esteem received broader support. Across all participants, both ethnicity- and sex-based discrimination of out-groups were associated with the maintenance of self-esteem, with the former showing a stronger association than the latter.
How does transcranial DC stimulation of the primary motor cortex alter regional neuronal activity in the human brain?

Transcranial direct current stimulation (tDCS) of the primary motor hand area (M1) can produce lasting polarity‐specific effects on corticospinal excitability and motor learning in humans. In 16 healthy volunteers, O positron emission tomography (PET) of regional cerebral blood flow (rCBF) at rest and during finger movements was used to map lasting changes in regional synaptic activity following 10 min of tDCS (± 1 mA). Bipolar tDCS was given through electrodes placed over the left M1 and right frontopolar cortex. Eight subjects received anodal or cathodal tDCS of the left M1, respectively. When compared to sham tDCS, anodal and cathodal tDCS induced widespread increases and decreases in rCBF in cortical and subcortical areas. These changes in rCBF were of the same magnitude as task‐related rCBF changes during finger movements and remained stable throughout the 50‐min period of PET scanning. Relative increases in rCBF after real tDCS compared to sham tDCS were found in the left M1, right frontal pole, right primary sensorimotor cortex and posterior brain regions irrespective of polarity. With the exception of some posterior and ventral areas, anodal tDCS increased rCBF in many cortical and subcortical regions compared to cathodal tDCS. Only the left dorsal premotor cortex demonstrated an increase in movement related activity after cathodal tDCS, however, modest compared with the relatively strong movement‐independent effects of tDCS. Otherwise, movement related activity was unaffected by tDCS. Our results indicate that tDCS is an effective means of provoking sustained and widespread changes in regional neuronal activity. The extensive spatial and temporal effects of tDCS need to be taken into account when tDCS is used to modify brain function.
Mitochondrial efficiency in rat skeletal muscle: influence of respiration rate, substrate and muscle type.

AIM To investigate the hypothesis that mitochondrial efficiency (i.e. P/O ratio) is higher in type I than in type II fibres during submaximal rates of respiration. METHODS Mitochondria were isolated from rat soleus and extensor digitorum longus (EDL) muscles, representing type I and type II fibres, respectively. Mitochondrial efficiency (P/O ratio) was determined with pyruvate (Pyr) or palmitoyl-l-carnitine (PC) during submaximal (constant rate of adenosine diphosphate infusion) and maximal (V(max), state 3) rates of respiration and fitted to monoexponential functions. RESULTS There was no difference in V(max) between PC and Pyr in soleus but in EDL V(max) with PC was only 58% of that with Pyr. The activity of 3-hydroxyacyl-CoA dehydrogenase was threefold higher in soleus than in EDL. P/O ratio at V(max) was 8-9% lower with PC [2.33 +/- 0.02 (soleus) and 2.30 +/- 0.02 (EDL)] than with Pyr [2.52 +/- 0.03 (soleus) and 2.54 +/- 0.03 (EDL)] but not different between the two muscles (P > 0.05). P/O ratio was low at low rates of respiration and increased exponentially when the rate of respiration increased. The asymptotes of the curves were similar to P/O ratio at V(max). P/O ratio at submaximal respirations was not different between soleus and EDL neither with Pyr nor with PC. CONCLUSION Mitochondrial efficiency, as determined in vitro, was not significantly different in the two fibre types neither at V(max) nor at submaximal rates of respiration. The low V(max) for PC oxidation in EDL may relate to low activity of beta-oxidation.
Audio source separation with a single sensor

In this paper, we address the problem of audio source separation with one single sensor, using a statistical model of the sources. The approach is based on a learning step from samples of each source separately, during which we train Gaussian scaled mixture models (GSMM). During the separation step, we derive maximum a posteriori (MAP) and/or posterior mean (PM) estimates of the sources, given the observed audio mixture (Bayesian framework). From the experimental point of view, we test and evaluate the method on real audio examples.
Consumer Acceptance and Use of Information Technology: Extending the Unified Theory of Acceptance and Use of Technology

This paper extends the unified theory of acceptance and use of technology (UTAUT) to study acceptance and use of technology in a consumer context. Our proposed UTAUT2 incorporates three constructs into UTAUT: hedonic motivation, price value, and habit. Individual differences--namely, age, gender, and experience--are hypothesized to moderate the effects of these constructs on behavioral intention and technology use. Results from a two-stage online survey, with technology use data collected four months after the first survey, of 1,512 mobile Internet consumers supported our model. Compared to UTAUT, the extensions proposed in UTAUT2 produced a substantial improvement in the variance explained in behavioral intention (56 percent to 74 percent) and technology use (40 percent to 52 percent). The theoretical and managerial implications of these results are discussed.
Pre-recombinational energy release and narrow features in the CMB spectrum

Energy release in the early Universe (z. 2× 10 6 ) should lead to some broad spectral distortion of the cosmic microwave background (CMB) radiation field, which can be characterized as y-type distortion when the injection process started at reds hifts z. 5× 10 4 . Here we demonstrate that if energy was released before the beginning of cosmological hydrogen recombination (z∼ 1400), closed loops of bound-bound and free-bound transitions in Hi and Heii lead to the appearance of (i) characteristic multiple narro w spectral features at dm and cm wavelengths, and (ii) a prominent sub-millimeter feature consisting of absorption and emission parts in the far Wien tail of CMB spectrum. The additional spectral features are generated in the pre-recombinational epoch of Hi (z& 1800) and Heii (z& 7000), and therefore differ from those arising due to normal cosmological recombination in the undisturbed CMB blackbody radiation field. We present the results of numerical computa tions including 25 atomic shells for both Hi and Heii, and discuss the contributions of several individual transitions in detail . As examples, we consider the case of instantaneous energy release (e.g. due to phase transitions) and exponential energy release because of long-lived decaying particles. Our computations show that due to possible pre-recombinational atomic transitions the variability o f the CMB spectral distortion increases when comparing with the distortions arising in the normal recombination epoch. The amplitude of the spectral features, both at low and high frequencies, dir ectly depends on the value of the y-parameter, which describes the intrinsic CMB spectral distortion resulting from the energy release. Also the time-dependence of the injection process play an important role, for example leading to non-trivial shifts of the quasi -periodic pattern at low frequencies along the frequency axis. The existence of these narrow spectral features would open an unique way to separate y-distortions due to pre-recombinational (1400 . z. 5× 10 4 ) energy release from those arising in the post-recombinational era at redshifts z. 800.