Miracle-dz/UniFAR-FTUs huggingface dataset:
Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint

Cenp-F is an unusual kinetochore protein in that it localizes to the nuclear matrix in interphase and the nuclear envelope at the G2/M transition; it is farnesylated and rapidly degraded after mitosis. We have recently shown that farnesylation of Cenp-F is required for G2/M progression, its localization to kinetochores, and its degradation. However, the role Cenp-F plays in mitosis has remained enigmatic. Here we show that, following repression of Cenp-F by RNA interference (RNAi), the processes of metaphase chromosome alignment, anaphase chromosome segregation and cytokinesis all fail. Although kinetochores attach to microtubules in Cenp-F-deficient cells, the oscillatory movements that normally occur following K-fibre formation are severely dampened. Consistently, inter-kinetochore distances are reduced. In addition, merotelic associations are observed, suggesting that whereas kinetochores can attach microtubules in the absence of Cenp-F, resolving inappropriate interactions is inhibited. Repression of Cenp-F does not appear to compromise the spindle checkpoint. Rather, the chromosome alignment defect induced by Cenp-F RNA interference is accompanied by a prolonged mitosis, indicating checkpoint activation. Indeed, the prolonged mitosis induced by Cenp-F RNAi is dependent on the spindle checkpoint kinase BubR1. Surprisingly, chromosomes in Cenp-F-deficient cells frequently show a premature loss of chromatid cohesion. Thus, in addition to regulating kinetochore-microtubule interactions, Cenp-F might be required to protect centromeric cohesion prior to anaphase commitment. Intriguingly, whereas most of the sister-less kinetochores cluster near the spindle poles, some align at the spindle equator, possibly through merotelic or lateral orientations.
Carefully Estimating the Incidence of Natalizumab-Associated PML

We show that the quarterly updates about the risk of PML during natalizumab therapy, while in principle helpful, underestimate the real incidences systematically and significantly. Calculating the PML incidences using an appropriate method and on realistic assumptions, we obtain estimates that are up to 80% higher. In fact, with the recent paper [Plavina et al 2014], our approximate incidences are up to ten times as high. The present article describes the shortcomings of the methods used in [Bloomgren et al 2012] and by Plavina et al for computing incidences, and demonstrates how to properly estimate the true (prospective) risk of developing PML during natalizumab treatment. One application is that the newest data concerning the advances in risk-mitigation through the extension of dosing intervals, although characterised as not quite statistically significant, are in fact significant. Lastly, we discuss why the established risk-stratification algorithms, even on assessing the PML incidences correctly, are no longer state-of-the-art; in the light of all the progress that has been made so far, already today it is possible to reliably identify over 95% of patients in whom (a personalised regimen of) natalizumab should be very safe.
A Shortest Dependency Path Based Convolutional Neural Network for Protein-Protein Relation Extraction

The state-of-the-art methods for protein-protein interaction (PPI) extraction are primarily based on kernel methods, and their performances strongly depend on the handcraft features. In this paper, we tackle PPI extraction by using convolutional neural networks (CNN) and propose a shortest dependency path based CNN (sdpCNN) model. The proposed method (1) only takes the sdp and word embedding as input and (2) could avoid bias from feature selection by using CNN. We performed experiments on standard Aimed and BioInfer datasets, and the experimental results demonstrated that our approach outperformed state-of-the-art kernel based methods. In particular, by tracking the sdpCNN model, we find that sdpCNN could extract key features automatically and it is verified that pretrained word embedding is crucial in PPI task.
Theology in multi-faith Religious Education: a taboo to be broken?

ABSTRACT This article discusses the place of ‘theology’ in multi-faith Religious Education (RE) in English schools without a religious affiliation, highlighting reasons for its sometimes taboo-status, particularly since the emergence of Ninian Smart’s phenomenological approach to Religious Studies in the late 1960s. The article explores a diversity of definitions of theology within specific professional and ecclesiastical discourses, and recasts recent debates by focusing not on whether theology and theological inquiry should contribute to so-called 'non-confessional' RE, but on how different forms of theology and theological inquiry might do so legitimately. In the process, the article challenges binary oppositions that have traditionally distinguished the disciplines of Theology from Religious Studies, and argues in favour of the application of various forms of theology and theological inquiry within a critical, dialogic and inquiry-led approach to multi-faith RE. What this might mean in practice is discussed with regard to three concepts: positionality, empathy and critique. Ultimately, multi-faith RE is characterised as occupying a liminal space betwixt and between disciplinary, interpretative and methodological perspectives involved in the study of religion(s) and worldview(s).
PENGARUH BRAND IMAGE TERHADAP BRAND LOYALTY MELALUI BRAND SATISFACTION PADA MEREK IMAPARTS

Di era globalisasi saat ini, persaingan di dunia bisnis menjadi semakin ketat terutama bagi perusahaan dengan bidang yang sejenis. Melihat pertumbuhan suku cadang yang pesat dan persaingan yang ketat membuat perusahaan untuk mengembangkan produknya dengan menjual produk dengan merek yang dibuat sendiri oleh pemilik. Penelitian ini menggunakan metode kuantitatif dengan teknik purposive sampling dan mengolah dengan partial least square (PLS) dengan alat bantu kuesioner. Sampel penelitian ini adalah 160 responden yang merupakan konsumen merek IMAparts dari CV. Indo Makmur Surabaya. Hasil analisa data disimpulkan bahwa ada citra merek berpengaruh terhadap kepuasan merek secara signifikan positif. Kepuasan merek berpengaruh terhadap loyalitas merek secara signifikan positif. Serta brand image tidak berpengaruh terhadap loyalitas merek.
Does market incompleteness matter for market microstructure ? *

Market incompleteness should matter in theory, but it is difficult to identify and measure the magnitude of its effects, especially on market microstructure. We use a natural experiment at the Tel Aviv Stock Exchange (TASE) to analyze how order submission patterns, trading and hedging strategies, and overall market impact are affected by market incompleteness. Options on the dollar are traded on Sundays on the TASE, while their underlying is not, which we use in a difference-in-differences setting with options on the equity index as control. Overall, our evidence suggests that market incompleteness has two majors effects on traders behaviors. First, likely because of expected higher adverse selection, traders change their patterns, resorting to higher strategic order splitting, nonetheless somewhat reducing their overall trading volumes. Second, because of actually lower information caused by the spot market closure, prices are in fact more efficient and volatility is lower, and aggregate trading costs during periods of incompleteness are slightly reduced, suggesting that informed trading plays a lesser role.
Free Fatty Acid Palmitate Impairs the Vitality and Function of Cultured Human Bladder Smooth Muscle Cells

Background Incidence of urinary tract infections is elevated in patients with diabetes mellitus. Those patients show increased levels of the saturated free fatty acid palmitate. As recently shown metabolic alterations induced by palmitate include production and secretion of the pro-inflammatory cytokine interleukine-6 (IL-6) in cultured human bladder smooth muscle cells (hBSMC). Here we studied the influence of palmitate on vital cell properties, for example, regulation of cell proliferation, mitochondrial enzyme activity and antioxidant capacity in hBSMC, and analyzed the involvement of major cytokine signaling pathways. Methodology/Principal Findings HBSMC cultures were set up from bladder tissue of patients undergoing cystectomy and stimulated with palmitate. We analyzed cell proliferation, mitochondrial enzyme activity, and antioxidant capacity by ELISA and confocal immunofluorescence. In signal transduction inhibition experiments we evaluated the involvement of NF-κB, JAK/STAT, MEK1, PI3K, and JNK in major cytokine signaling pathway regulation. We found: (i) palmitate decreased cell proliferation, increased mitochondrial enzyme activity and antioxidant capacity; (ii) direct inhibition of cytokine receptor by AG490 even more strongly suppressed cell proliferation in palmitate-stimulated cells, while counteracting palmitate-induced increase of antioxidant capacity; (iii) in contrast knockdown of the STAT3 inhibitor SOCS3 increased cell proliferation and antioxidant capacity; (iv) further downstream JAK/STAT3 signaling cascade the inhibition of PI3K or JNK enhanced palmitate induced suppression of cell proliferation; (v) increase of mitochondrial enzyme activity by palmitate was enhanced by inhibition of PI3K but counteracted by inhibition of MEK1. Conclusions/Significance Saturated free fatty acids (e.g., palmitate) cause massive alterations in vital cell functions of cultured hBSMC involving distinct major cytokine signaling pathways. Thereby, certain cytokines might counteract the palmitate-induced downregulation of cell proliferation and vitality. This could be an important link to clinical findings of increased risk of metabolic related bladder diseases such as overactive bladder (OAB) and bladder pain syndrome/interstitial cystitis (BPS/IC).
Miniaturized radioisotope solid state power sources

Electrical power requirements for the next generation of deep space missions cover a wide range from the kilowatt to the milliwatt. Several of these missions call for the development of compact, low weight, long life, rugged power sources capable of delivering a few milliwatts up to a couple of watts while operating in harsh environments. Advanced solid state thermoelectric microdevices combined with radioisotope heat sources and energy storage devices such as capacitors are ideally suited for these applications. By making use of macroscopic film technology, microgenrators operating across relatively small temperature differences can be conceptualized for a variety of high heat flux or low heat flux heat source configurations. Moreover, by shrinking the size of the thermoelements and increasing their number to several thousands in a single structure, these devices can generate high voltages even at low power outputs that are more compatible with electronic components. Because the miniaturization of state-.
Helium Diffusion Age of 6,000 Years Supports Accelerated Nuclear Decay

Experiments co-sponsored by the Creation Research So- ciety show that helium leakage deflates radioisotopic ages. In 1982 Robert Gentry found amazingly high re- tentions of nuclear-decay-generated helium in micro- scopic zircons (ZrSiO 4 crystals) recovered from a bore- hole in hot Precambrian granitic rock at Fenton Hill, NM. We contracted with a high-precision laboratory to measure the rate of helium diffusion out of the zircons. The initial results were very encouraging. Here we re- port newer zircon diffusion data that extend to the lower temperatures (100o to 277o C) of Gentry's retention data. The measured rates resoundingly confirm a numerical prediction we made based on the reported retentions and a young age. Combining rates and retentions gives a helium diffusion age of 6,000 ± 2,000 years. This con- tradicts the uniformitarian age of 1.5 billion years based on nuclear decay products in the same zircons. These data strongly support our hypothesis of episodes of highly accelerated nuclear decay occurring within thou- sands of years ago. Such accelerations shrink the radio- isotopic "billions of years" down to the 6,000-year timescale of the Bible. (§ is section of reference being cited.) Introductionthe deep blue skies of northern New Mexico in the fall of 1974, drillmen labored to extract cores from a bore- hole called GT-2 (Figure 1) nearly three miles deep. The site was Fenton Hill, on the west flank of the Valles volca- nic caldera in the pine-covered Jemez Mountains. Two dozen miles to the east, geoscientists at Los Alamos Na- tional Laboratory analyzed the drill cores, investigating whether the hot, dry rock would be suitable for providing geothermal energy. The geoscientists identified the rock as biotite grano- diorite, a granitic rock containing shiny flecks of a black mica called biotite. They crushed a core sample from a depth of 2,900 meters and extracted microscopic crystals of zirconium silicate (ZrSiO 4 ) embedded in the biotite. These crystals, called zircons, were radioactive, containing high amounts of uranium and thorium relative to the rest of the rock, as is usual for that mineral. Comparing two
State Weakness and Regional Security Instability: Evidence from Africa’s Lake Chad Region

There is a considerable evidence showing that many states in the Global South are very weak, and therefore struggle to carry out basic responsibilities of statehood. While a handful of studies have examined problems associated with state weakness, there is a paucity of scholarly literature that thoroughly explores its empirical implications on regional security. It is on this note that this article draws on the contemporary developments in the Lake Chad region to elucidate the nodes that connect state weakness and regional security instability. It argues that any state that cannot efficaciously control its borders, promptly respond to security emergencies and demonstrate substantial institutional capacity in addressing citizens’ needs is vulnerable to create regional insecurity, especially when the neighbouring states share similar attributes. The article concludes that an alternative approach to ensuring lasting regional security in such regions, especially in the present Lake Chad region, is deliberate commitment to state-building.
Exploratory Factor Analysis of the Chilean Deafness Attitude Scale.

Abstract: Negative attitudes mean a barrier for People with Disability (PwD), but attitudes towards Deaf people are structurally different from the rest of the PwD. The aim of this study is to evaluate the factorial structure and internal reliability of the Attitudes towards Deafness Scale (ADS) applied in health professionals from Concepcion, Chile. The ADS was translated to Spanish and back-translated to English, then reviewed by public health experts, and a pilot application was carried out to 15 health professional to make final modifications. The Chilean ADS version was applied to 182 health professional from primary care centers and academics working in health schools. Reliability analysis with estimation of the Cronbach’s alpha coefficient, and exploratory factor analysis were made. The data presented adequate values to perform exploratory factor analysis (KMO=0.73; Bartlett’s sphericity test p <0.00001). A maximum likelihood extraction method and a Quartimax rotation method with Kaiser standardization were used for a four-factor model, in which 11 items presented loads of less than 0.5, so the final model was left with 11 items. A Cronbach’s alpha coefficient of 0.70 was determined in the final model, with an alpha of 0.77 for the first factor, 0.55 for the second, 0.77 for the third, and 0.61 for the fourth. The exploratory factor analysis suggested a four-dimensional structure for ADS Chilean version. The four subscales and the scale in general presented an adequate Cronbach’s alpha, suggesting acceptable internal consistency.
Autonomy Support, Psychological Needs Satisfaction, School Engagement and Academic Success: A Mediation Model

School engagement is a construct of relevance in education and educational psychology, as it has been related to multiple educational constructs and outcomes: school drop-out, satisfaction with school, disruptive behavior, motivational climate, teacher-student relationships, or academic progress and achievement. The current research surveyed 2034 Angolan students and 2302 Dominican Republic students in order to predict academic achievement. The model tested was supported by the data in both samples, and the chain of explicative effects hold again in both samples. School engagement was a powerful mediator among needs satisfaction and academic success. Results are discussed in light of existing literature, and limitations and future directions of research are also highlighted.
Institutional Arrangements and Fiscal Performance: The Latin American Experience

This paper considers whether institutional factors, in this instance electoral systems and procedures, affect Latin American countries' fiscal performance as measured by the size of the public sector, fiscal deficits, the size of the public debt, and the degree of procyclality of fiscal policy. The authors find that electoral systems characterized by large district magnitude and high political fragmentation have larger governments, larger deficits, and more procyclical fiscal policies. Transparent and hierarchical budget procedures, on the other hand, lead to lower deficits and levels of debt.
The pianist's acoustical and motional expressions in the live performance of Schumann's Träumerei

According to previous studies, expert performers construct their acoustical (i.e. durational, dynamical) and visual (i.e. motional) expressions by highlighting the structurally important points. However, it is still unclear whether such expressions are influenced by the presence of the audience. In the present study, we examined the effects of context (i.e. audience present versus audience absent ) on expert pianists’ acoustical and motional expressions in their performances of Robert Schumann’s Traumerei. In the experiment, 13 pianists performed six pieces including Traumerei in front of 11-23 audience members. Either before or after the live performance, each pianist performed the same pieces without any audience. According to the functional data analyses for the duration, the dynamic range, and the motional range, the trough and the peak points within each parameter were in phase with the boundaries between the sections regardless of the context. The analyses also showed that the pianists amplified the ranges of the expressions at the trough and the peak within each parameter in front of their audiences. These results suggest that sharing time and space with the audience amplifies the pianists’ expressions in performing structurally salient points (e.g. phrase endings).
The diagnostic utility of serum IgG4 concentrations in IgG4-related disease

Objectives We evaluated the sensitivity, specificity and positive and negative predictive values of elevated serum IgG4 concentrations for the diagnosis of IgG4-RD. Methods Between 2001 and 2011, 190 unique patients had elevated serum IgG4 measurements. We reviewed electronic medical records to determine the indication for IgG4 measurement and underlying clinical diagnosis. Additionally, we reviewed the records of 190 other randomly selected patients from a pool of 3360 with normal results, to evaluate test characteristics of the IgG4 measurement. Results Among 380 patients analysed, 72 had either probable or definite IgG4-RD. Sixty-five of the 72 IgG4-RD patients had elevated serum IgG4 concentrations (mean: 405 mg/dL; range 140–2000 mg/dL), for a sensitivity of 90%. Among the 308 subjects without IgG4-RD, 125 had elevated IgG4 (mean: 234 mg/dL; range 135–1180 mg/dL) and 183 had normal IgG4 concentrations, for a specificity of 60%. The negative predictive value of a serum IgG4 assay was 96%, but the positive predictive value only 34%. Analysis of the serum IgG4/total IgG ratio did not improve these test characteristics. Doubling the cutoff for IgG4 improved specificity (91%) but decreased sensitivity to 35%. Discussion Multiple non-IgG4-RD conditions are associated with elevated serum IgG4, leading to poor specificity and low positive predictive value for this test. A substantial subset of patients with biopsy-proven IgG4-RD do not have elevated serum IgG4. Neither doubling the cutoff for serum IgG4 nor examining the serum IgG4/IgG ratio improves the overall test characteristics for the diagnosis of IgG4-RD.
Food in fashion modelling: Eating as an aesthetic and moral practice

This paper investigates the relation between food, the body and morality in fashion modelling. More than has been recognized so far, eating is a continuous form of body work that is decidedly essential to aesthetic labour. Against the backdrop of slender aesthetics, models are purposefully socialized into remaining or becoming slender, through food beliefs inducing them to eat in specific ways. Food is classified into good and bad categories, and believed to affect male and female bodies differently. But other than to aesthetics or gender, considering ‘what (not) to eat’ links to morality, enabling models to draw symbolic boundaries between themselves and others. These show two main moral imperatives: models should eat controlled and effortlessly. Solving this moral paradox, models normalize and conceal controlled eating. Ultimately, the fashion modelling food system preoccupies models with self-surveillance and reinforces power inequalities between models and other professionals.
Retrospectively and Prospectively Modulated Hippocampal Place Responses Are Differentially Distributed along a Common Path in a Continuous T-Maze

Hippocampal place responses can be prospectively or retrospectively modulated by the animal's future or prior trajectory. Two main hypotheses explain this. The “multiple-map hypothesis” switches between different maps for different trajectories (rate remapping). In contrast, in the “buffer hypothesis,” the hippocampus encodes an ongoing representation that includes the recent past and/or the impending future choice. This study examines the distribution of prospective and retrospective responses distributed along a common path in a continuous T-maze (providing all four combinations of provenance and destination) during a visual discrimination task. The multiple-map hypothesis predicts either uniform distributions or concerted shifts about a task-decision relevant point, whereas the buffer hypothesis predicts a time-limited overexpression around choice points (with retrospective responses after the central arm entry point and prospective responses nearer its exit). Here bilateral recordings in the dorsal CA1 region of the rat hippocampus show that retrospective responses were twice as prevalent as prospective responses. Furthermore, retrospective and prospective modulations have distinct spatial distributions, with retrospective primarily in the first two-thirds of the central arm and prospective restricted to the last third. To test for possible trial-by-trial remapping in relation to the two-thirds transition point, data from the first and second halves of the sessions were compared. Backward drift of path-modulated activity was significant only for retrospective, but not prospective, fields. Thus, these data are more consistent with the buffer hypothesis. Retrospective and prospective modulation would then participate in a single hippocampal representation of spatial and behavioral context.