Miracle-dz/UniFAR-FTUs huggingface dataset:
The differences between the localizations of MUC1, MUC5AC, MUC6 and osteopontin in quail proventriculus and gizzard may be a reflection of functional differences of stomach parts

Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer and are produce by many epithelial tissues in vertebrates. Osteopontin (OPN) is an adhesive phosphorylated glycoprotein that is expressed by a broad range of tissues and cells. Although gastric mucins MUC1, MUC5AC, MUC6 and OPN have been widely used in histological studies and in diagnostic pathology in order to diagnose gastric carcinomas, their localizations in the stomach of quail have not yet been studied. In this study, the localizations of MUC1, MUC5AC, MUC6 and OPN in the proventriculus and gizzard of Japanese quail during the post‐hatching period were compared at light microscope levels by applying immunohistochemical methods. In all ages studied, the immunoreactivity of MUC5AC was present in the lining epithelium of both folds and superficial proventricular glands in the proventriculus, whereas MUC1, MUC6 and OPN reactivity was found in the oxynticopeptic cells of profound proventricular glands. In addition, some cells in the fold epithelium of the proventriculus showed a positive reaction to OPN. The immunoreactivity of MUC1 in gizzard was different from that of MUC5AC. Although MUC5AC was expressed in the cells of both the surface epithelium and profound glands of the gizzard, MUC1 was only localized in the profound glands of the gizzard. However, MUC6 and OPN immunoreactivity was absent in the gizzard. The results indicated that the differences between the localizations of MUC1, MUC5AC, MUC6 and OPN in quail proventriculus and gizzard may be a reflection of functional differences of stomach parts. Although the biological significances of the expressions of MUC1, MUC5AC, MUC6 and OPN in the quail stomach remains unknown, these notable glycoproteins may be associated with barrier function, host defence, and/or secretion.
Assessment of Palm Press Fibre and Sawdust-Based Substrate Formulas for Efficient Carpophore Production of Lentinus squarrosulus (Mont.) Singer

Abstract Development of efficient substrate formulas to improve yield and shorten production time is one of the prerequisites for commercial cultivation of edible mushrooms. In this study, fifteen substrate formulas consisting of varying ratios of palm press fibre (PPF), mahogany sawdust (MS), Gmelina sawdust, wheat bran (WB), and fixed proportions of 1% calcium carbonate (CaCO3) and 1% sucrose were assessed for efficient Lentinus squarrosulus production. Proximate compositions of mushrooms produced on the different substrate formulas were also analysed and compared. Substrate formulations containing 85% PPF, 13% WB, 1% CaCO3, and 1% sucrose were found to produce the highest carpophore yield, biological efficiency and size (206.5 g/kg, 61.96%, and 7.26 g, respectively). Days to production (first harvest) tended to increase with an increase in the amount of WB in the substrate formulas, except for PPF based formulas. The addition of WB in amounts equivalent to 8~18% in substrate formulas containing 80~90% PPF resulted in a decrease in the time to first harvest by an average of 17.7 days compared to 80~90% MS with similar treatment. Nutritional content of mushrooms was affected by the different substrate formulas. Protein content was high for mushrooms produced on formulas containing PPF as the basal substrate. Thus, formulas comprising PPF, WB, CaCO3, and sucrose at 85% : 13% : 1% : 1%) respectively could be explored as starter basal ingredients for efficient large scale production of L. squarrosulus.
Epidemic Investigation of the Jaundice Outbreak in Girdharnagar, Ahmedabad, Gujarat, India, 2008

Background: Since 1976, seven outbreaks of hepatitis E occurred in Ahmedabad. Clusters of jaundice cases were reported on June 19, 2008, by a civic center, Girdharnagar ward, Ahmedabad. Objectives: The objectives were as follows: (1) to identify the etiological agent, source of outbreak, and mode of transmission; (2) to propose a control measure based on the outbreak investigation. Materials and Methods: We defined a case as an acute illness with (a) a discrete onset of symptoms and (b) jaundice or elevated serum aminotransferase levels, from March to September 2008 in the households of the Girdharnagar ward. We collected data through a door-to-door survey and hospital records. We described the outbreak in terms of time, place, and person. We collected laboratory investigation reports of case patients admitted to the civil hospital. To test our hypothesis we conducted a retrospective cohort study to find out the relative risk for hepatitis. We conducted environment investigation to find out the source of contamination of water supply. Results: A total 233 case patients of hepatitis were identified with the attack rate of 10.9/1000 population. Cases were reported in all the age groups with a higher attack rate in the age group of 20-29 years (18.5/1000). Out of 17 case patients, 16 were positive for the hepatitis E IgM antibody. The attack rate was two times more among those who were exposed to the leaking pipeline than the non-exposed (RR=2.3, 95% CI 1.76, 2.98). Environmental investigation also confirmed the sewage contamination of drinking water in the distribution system. Conclusion: The outbreak was due to hepatitis E virus. We recommended a temporary alternative water supply, repair of the leakages, and water quality surveillance.
Modeling and simulation approaches in the resin transfer molding process: A review

A review of current approaches in modeling and simulation of the resin transfer molding (RTM) process is presented. The processing technology of RTM is discussed and some available experimental techniques to monitor the process cycle are presented. A master model is proposed for the entire process cycle consisting of mold filling and curing stages. This master model contains the fundamental and constitutive sub-models for both stages. The key elements of the master model discussed in this study are: flow, heat and mass balance equations for fundamental sub-models, permeability, cure kinetics, resin viscosity and void formation for constitutive sub-models. At the end, numerical methods widely used to simulate the filling process are presented and published simulation results of mold filling and process cycle are reviewed.
An automatic kernel parameter selection method for kernel nonparametric weighted feature extraction with the RBF kernel for hyperspectral image classification

For hyperspectral image classification, feature extraction is a crucial pre-process for avoiding the Hughes phenomena. Some feature extraction methods such as linear discriminant analysis (LDA), nonparametric weighted feature extraction (NWFE), and their kernel versions, generalized discriminant analysis (GDA) and kernel nonparametric weighted feature extraction method (KNWFE) have been shown that they can improve the classification performance. However, for GDA and KNWFE, it is hard to find the suitable kernel parameters. Hence, although they have been published about 14 or 6 years, respectively, researchers rarely implement them for dealing with hyperspectral image classification problem. An automatic kernel parameter selection method (APS) was proposed to predetermine the appropriate radial basis function (RBF) kernel for support vector machine (SVM) and GDA. In this study, APS was applied to find the suitable RBF kernel function for KNWFE. From the experiment results on PAVIA data set, the classification performance of KNWFE still outperforms those of GDA [10] and SVM [10]. The most important of this research, the kernel parameters of GDA and KNWFE based on RBF kernel can be “automatically” determined and the researcher can implement them directly without tuning the kernel parameter.
Regulation of Tumor Necrosis Factor Alpha Promoter by Human Parvovirus B19 NS1 through Activation of AP-1 and AP-2

ABSTRACT Human parvovirus B19 frequently causes acute and chronic arthritis in adults. The molecular mechanism of B19 arthritis, however, remains poorly understood. We previously showed that the transmission of B19 from rheumatoid synoviocytes to monocytic cells is associated with enhanced secretion of tumor necrosis factor alpha (TNF-α), which triggers inflammation, and interleukin-6. To determine the role of B19 in the production of TNF-α, we focused on the function of its nonstructural protein, NS1, and established monocytic U937 lines transduced with the NS1 gene under the control of an inducible promoter. Production of TNF-α mRNA and protein was elevated in a manner associated with NS1 expression. Reporter assays revealed that AP-1 and AP-2 motifs on the TNF-α promoter were responsible for NS1-mediated up-regulation. Electrophoretic mobility shift assay showed specific binding of nuclear proteins from NS1 gene-transduced cells with the AP-1 or AP-2 probe. Antibodies against transcription factors AP-1 and AP-2 and anti-NS1 antibody inhibited the binding of nuclear proteins to the corresponding probes. These data indicate that NS1 up-regulates TNF-α transcription via activation of AP-1 and AP-2 in monocytic cells. The molecular mechanisms of NS1-mediated TNF-α expression would explain the pathogenesis of B19-associated inflammation.
Debriefing critical incidents in the paediatric emergency department: Current practice and perceived needs in Australia and New Zealand

Anecdotally critical incident debriefing (CID) is an important topic for staff in paediatric ED. The present study aimed to determine current baseline CID practices and perceived needs of ED staff. A questionnaire regarding CID practice was circulated to all 13 Paediatric Research in Emergency Departments International Collaborative (PREDICT) sites in Australia and New Zealand (including all tertiary paediatric ED), and completed by 1 senior doctor and 1 senior nurse. All PREDICT sites participated (13 nurses, 13 doctors). Seventy per cent did not currently have a hospital protocol on debriefing and 90% did not have ED‐specific guidelines. The most commonly debriefed topics were death of a patient, multi‐trauma and sudden infant death syndrome, also ranked highest in importance for debriefing. The median reported debriefs per department were 4 per year (range 0–12), all conducted within a week of the CI with half within 24 h. ED workers most likely to be invited to the CID session were doctors, nurses and social workers (96%). Debriefing was mostly conducted internally (62%) and most likely facilitated by a doctor (81%) or nurse (54%). Debriefing addressed both clinical and emotional issues (89%) within the same session (69%). Debriefing was rated as very important, median of 8/10 by doctors and 10/10 by nurses. Almost 90% of those surveyed indicated that they would like a CID programme and guidelines for their department. Debriefing is perceived as important by senior ED clinicians, yet few ED have formalized guidelines or programmes. Best‐practice guidelines should be developed.
Incidental brane defects

In the models of brane construction, the isometry of a compactified space might be broken by branes. In four-dimensional effective Lagrangian, the breaking of the isometry is seen as the spontaneous breaking of the corresponding effective symmetry. Then it seems natural to expect that there are various kinds of defects that will be implemented by the spontaneous symmetry breaking. These defects are parametrized by the brane positions. In this paper we consider two kinds of such ``brane defects'', which are formed by the local fluctuations of the locations of branes along their transversal directions. The fluctuation of a brane position might leads to winding (or wraping) around a non-contractible circle of the compactified space. These ``primary'' brane defects are already discussed by several authors. On the other hand, if there are multiple branes in the compactified space and their configuration in a compactified space is determined by the potential that depends only on their relative positions, one might find incidental symmetry in the effective potential, which is spontaneously broken by branes. We examined the latter ``incidental'' symmetry breakings and stable defect configurations. We paid special attention to the difference between ``primary'' brane defects.
Neuro-Fuzzy modeling of torsional strength of RC beams

This paper presents Neuro-Fuzzy (NF) based empirical modelling of torsional strength of RC beams for the first time in literature. The proposed model is based on fuzzy rules. The experimental database used for NF modelling is collected from the literature consisting of 76 RC beam tests. The input variables in the developed rule based on NF model are cross-sectional area of beams, dimensions of closed stirrups, spacing of stirrups, cross-sectional area of one-leg of closed stirrup, yield strength of stirrup and longitudinal reinforcement, steel ratio of stirrups, steel ratio of longitudinal reinforcement and concrete compressive strength. According to the selected variables, the formulated NFs were trained by using 60 of the 76 sample beams. Then, the method was tested with the other 16 sample beams. The accuracy rates were found to be about 96% for total set. The performance of accuracy of proposed NF model is furthermore compared with existing design codes by using the same database and found to be by far more accurate. The use of NF provided an alternative way for estimating the torsional strength of RC beams. The outcomes of this study are quite satisfactory which may serve NF approach to be widely used in further applications in the field of reinforced concrete structures.
Training of Working Memory Impacts Structural Connectivity

Working memory is the limited capacity storage system involved in the maintenance and manipulation of information over short periods of time. Individual capacity of working memory is associated with the integrity of white matter in the frontoparietal regions. It is unknown to what extent the integrity of white matter underlying the working memory system is plastic. Using voxel-based analysis (VBA) of fractional anisotropy (FA) measures of fiber tracts, we investigated the effect of working memory training on structural connectivity in an interventional study. The amount of working memory training correlated with increased FA in the white matter regions adjacent to the intraparietal sulcus and the anterior part of the body of the corpus callosum after training. These results showed training-induced plasticity in regions that are thought to be critical in working memory. As changes in myelination lead to FA changes in diffusion tensor imaging, a possible mechanism for the observed FA change is increased myelination after training. Observed structural changes may underlie previously reported improvement of working memory capacity, improvement of other cognitive functions, and altered functional activity following working memory training.
Differential Receptor Tyrosine Kinase PET Imaging for Therapeutic Guidance

Inhibitors of the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway hold promise for the treatment of breast cancer, but resistance to these treatments can arise via feedback loops that increase surface expression of the receptor tyrosine kinases (RTK) epidermal growth factor receptor 1 (EGFR) and human epidermal growth factor receptor 3 (HER3), leading to persistent growth pathway signaling. We developed PET probes that provide a method of imaging this response in vivo, determining which tumors may use this escape pathway while avoiding the need for repeated biopsies. Methods: Anti-EGFR-F(ab′)2 and anti-HER3-F(ab′)2 were generated from monoclonal antibodies by enzymatic digestion, conjugated to DOTA, and labeled with 64Cu. A panel of breast cancer cell lines was treated with increasing concentrations of the AKT inhibitor GDC-0068 or the PI3K inhibitor GDC-0941. Pre- and posttreatment expression of EGFR and HER3 was compared using Western blot and correlated to probe accumulation with binding studies. Nude mice xenografts of HCC-70 or MDA-MB-468 were treated with either AKT inhibitor or PI3K inhibitor and imaged with either EGFR or HER3 PET probe. Results: Changes in HER3 and EGFR PET probe accumulation correlate to RTK expression change as assessed by Western blot (R2 of 0.85–0.98). EGFR PET probe PET/CT imaging of HCC70 tumors shows an SUV of 0.32 ± 0.03 for vehicle-, 0.50 ± 0.01 for GDC-0941–, and 0.62 ± 0.01 for GDC-0068–treated tumors, respectively (P < 0.01 for both comparisons to vehicle). HER3 PET probe PET/CT imaging of MDAMB468 tumors shows an SUV of 0.35 ± 0.02 for vehicle- and 0.73 ± 0.05 for GDC-0068–treated tumors (P < 0.01). Conclusion: Our imaging studies, using PET probes specific to EGFR and HER3, show that changes in RTK expression indicative of resistance to PI3K and AKT inhibitors can be seen within days of therapy initiation and are of sufficient magnitude as to allow reliable clinical interpretation. Noninvasive PET monitoring of these RTK feedback loops should help to rapidly assess resistance to PI3K and AKT inhibitors and guide selection of an appropriate combinatorial therapeutic regimen on an individual patient basis.
Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism

Eosinophil cationic protein (ECP), a cytotoxic protein contained in eosinophils granules, can contribute to various inflammatory responses. Although Helicobacter pylori infection increases infiltration of eosinophils, the mechanisms of eosinophil degranulation by H. pylori infection are largely unknown. The goal of this study was to investigate the role of H. pylori outer membrane vesicles (OMVs) in modulating eosinophil degranulation. We found that eosinophils treated with H. pylori OMVs released significantly more ECP compared with untreated controls. In addition, eosinophils cocultured with OMV-preexposed primary gastric epithelial cells exhibited significantly increased ECP release. Similarly, eosinophils cocultured with culture supernatant (CM) from primary gastric epithelial cells exposed to OMVs (OMV-CM) released significantly higher amounts of ECP compared with eosinophils cocultured with CM from unexposed control cells. Furthermore, OMVs and OMV-CM both induced the upregulation of ICAM-1 on gastric epithelial cells and β2 integrin CD11b on eosinophils. In addition, both transduction of ICAM-1 shRNA into gastric epithelial cells and treatment with neutralizing mAbs to CD18 significantly decreased OMV-mediated or OMV-CM-mediated release of ECP. These results suggest that the eosinophil degranulation response to H. pylori OMVs occurs via a mechanism that is dependent on both β2 integrin CD11/CD18 and ICAM-1.
Design and analysis of a flow control scheme over wireless networks

This paper studies the problem of congestion control on wireless networks. A dynamical model for the end‐to‐end network flow control that exploits the differentiation between congestion loss and physical channel error loss is proposed. The introduction of a specific wireless model is motivated by the distinctive presence of channel errors, which are often not known exactly. We assume that each wireless link is associated with an additional error function that depends on the current flow along the link and that accounts for the packet loss rate caused by the physical channel. This leads to a new dynamic flow control scheme that naturally extends a known mathematical model for the fluid‐flow approximation of the Transmission Control Protocol for wireline networks. The main objective of this work is to study the dynamical properties of the new model: we analyze its nonlinear dynamics, derive its stability properties, and study its robustness to delays. We also present and discuss some ns‐2 simulations of its dynamics. This work additionally looks at the actual implementation of the proposed scheme: by requiring only modifications to the application layer rather than the transport one, no alterations to the network infrastructure or transport protocols are needed. The article argues that the new scheme appears to be not only theoretically meaningful but also practically relevant for an application layer implementation. Copyright © 2011 John Wiley & Sons, Ltd.
Predictor-Based Remote Tracking Control of a Mobile Robot

In this paper, we address the tracking control problem for a unicycle-type mobile robot which is remotely controlled by a two-channel, delay-inducing communication network. A predictor-based control strategy capable of controlling the negative effects of the time-delay is proposed. Moreover, conditions are provided guaranteeing the local or global asymptotic stability of the closed-loop system up to a maximum admissible delay. The applicability of the proposed predictor-controller combination is demonstrated using an interconnected robotic platform located partly in Eindhoven, the Netherlands, and Tokyo, Japan.
The impact of age on cerebral perfusion, oxygenation and metabolism during exercise in humans

Age is one of the most important risk factors for dementia and stroke. Examination of the cerebral circulatory responses to acute exercise in the elderly may help to pinpoint the mechanisms by which exercise training can reduce the risk of brain diseases, inform the optimization of exercise training programmes and assist with the identification of age‐related alterations in cerebral vascular function. During low‐to‐moderate intensity dynamic exercise, enhanced neuronal activity is accompanied by cerebral perfusion increases of ∼10–30%. Beyond ∼60–70% maximal oxygen uptake, cerebral metabolism remains elevated but perfusion in the anterior portion of the circulation returns towards baseline, substantively because of a hyperventilation‐mediated reduction in the partial pressure of arterial carbon dioxide ( P aC O2 ) and cerebral vasoconstriction. Cerebral perfusion is lower in older individuals, both at rest and during incremental dynamic exercise. Nevertheless, the increase in the estimated cerebral metabolic rate for oxygen and the arterial–internal jugular venous differences for glucose and lactate are similar in young and older individuals exercising at the same relative exercise intensities. Correction for the age‐related reduction in P aC O2 during exercise by the provision of supplementary CO2 is suggested to remove ∼50% of the difference in cerebral perfusion between young and older individuals. A multitude of candidates could account for the remaining difference, including cerebral atrophy, and enhanced vasoconstrictor and blunted vasodilatory pathways. In summary, age‐related reductions in cerebral perfusion during exercise are partly associated with a lower P aC O2 in exercising older individuals; nevertheless the cerebral extraction of glucose, lactate and oxygen appear to be preserved.
Successful angioplasty of three cases of coronary artery dissections using hydrophilic wires

Three cases of successful angioplasty of high-grade coronary dissections using hydrophilic wires were reported. Our first case had edge dissection after a stent deployed in the left anterior descending artery, after which we found it impossible to track the second stent over the regular wires, and which was successful when we tried with a stiffer hydrophilic wire. The second had spontaneous coronary artery dissections (SCAD), and the third case was a complicated plaque with multiple stenotic and ectatic segments along with dissection and successful angioplasty carried out using the same wires and without additional hardware. These wires also provided adequate support in tracking the required balloons and stents