Miracle-dz/UniFAR-FTUs huggingface dataset:
Ammonia detection using optical reflectance from porous silicon formed by metal-assisted chemical etching

An impact of morphology on reflectance of porous silicon was investigated. Depending on the metal-assisted chemical etching conditions the macro- micro structures could be formed. The reflectance properties of various porous silicon structures after ammonia adsorption were investigated. It was shown that increasing of ammonia concentration in the measurement camber leads to an increase of the reflectance. The most sensitive structures for ammonia detection are porous silicon having approximately size of pores - 10-15 μm. A fast response of porous silicon on the adsorption of ammonia molecules may be used for development of new sensors.
A Structural View of Xenophagy, a Battle between Host and Microbes

The cytoplasm in mammalian cells is a battlefield between the host and invading microbes. Both the living organisms have evolved unique strategies for their survival. The host utilizes a specialized autophagy system, xenophagy, for the clearance of invading pathogens, whereas bacteria secrete proteins to defend and escape from the host xenophagy. Several molecules have been identified and their structural investigation has enabled the comprehension of these mechanisms at the molecular level. In this review, we focus on one example of host autophagy and the other of bacterial defense: the autophagy receptor, NDP52, in conjunction with the sugar receptor, galectin-8, plays a critical role in targeting the autophagy machinery against Salmonella; and the cysteine protease, RavZ secreted by Legionella pneumophila cleaves the LC3-PE on the phagophore membrane. The structure-function relationships of these two examples and the directions of future research will be discussed.
Variation in growth rate, carbon assimilation, and photosynthetic efficiency in response to nitrogen source and concentration in phytoplankton isolated from upper San Francisco Bay

Six species of phytoplankton recently isolated from upper San Francisco Bay were tested for their sensitivity to growth inhibition by ammonium (NH4+), and for differences in growth rates according to inorganic nitrogen (N) growth source. The quantum yield of photosystem II (Fv/Fm) was a sensitive indicator of NH4+ toxicity, manifested by a suppression of Fv/Fm in a dose‐dependent manner. Two chlorophytes were the least sensitive to NH4+ inhibition, at concentrations of >3,000 μmoles NH4+ · L−1, followed by two estuarine diatoms that were sensitive at concentrations >1,000 μmoles NH4+ · L−1, followed lastly by two freshwater diatoms that were sensitive at concentrations between 200 and 500 μmoles NH4+ · L−1. At non‐inhibiting concentrations of NH4+, the freshwater diatom species grew fastest, followed by the estuarine diatoms, while the chlorophytes grew slowest. Variations in growth rates with N source did not follow taxonomic divisions. Of the two chlorophytes, one grew significantly faster on nitrate (NO3−), whereas the other grew significantly faster on NH4+. All four diatoms tested grew faster on NH4+ compared with NO3−. We showed that in cases where growth rates were faster on NH4+ than they were on NO3−, the difference was not larger for chlorophytes compared with diatoms. This holds true for comparisons across a number of culture investigations suggesting that diatoms as a group will not be at a competitive disadvantage under natural conditions when NH4+ dominates the total N pool and they will also not have a growth advantage when NO3− is dominant, as long as N concentrations are sufficient.
Joint Demosaicing and Denoising with Perceptual Optimization on a Generative Adversarial Network

Image demosaicing - one of the most important early stages in digital camera pipelines - addressed the problem of reconstructing a full-resolution image from so-called color-filter-arrays. Despite tremendous progress made in the pase decade, a fundamental issue that remains to be addressed is how to assure the visual quality of reconstructed images especially in the presence of noise corruption. Inspired by recent advances in generative adversarial networks (GAN), we present a novel deep learning approach toward joint demosaicing and denoising (JDD) with perceptual optimization in order to ensure the visual quality of reconstructed images. The key contributions of this work include: 1) we have developed a GAN-based approach toward image demosacing in which a discriminator network with both perceptual and adversarial loss functions are used for quality assurance; 2) we propose to optimize the perceptual quality of reconstructed images by the proposed GAN in an end-to-end manner. Such end-to-end optimization of GAN is particularly effective for jointly exploiting the gain brought by each modular component (e.g., residue learning in the generative network and perceptual loss in the discriminator network). Our extensive experimental results have shown convincingly improved performance over existing state-of-the-art methods in terms of both subjective and objective quality metrics with a comparable computational cost.
AMPK signaling to acetyl-CoA carboxylase is required for fasting- and cold-induced appetite but not thermogenesis

AMP-activated protein kinase (AMPK) is a known regulator of whole-body energy homeostasis, but the downstream AMPK substrates mediating these effects are not entirely clear. AMPK inhibits fatty acid synthesis and promotes fatty acid oxidation by phosphorylation of acetyl-CoA carboxylase (ACC) 1 at Ser79 and ACC2 at Ser212. Using mice with Ser79Ala/Ser212Ala knock-in mutations (ACC DKI) we find that inhibition of ACC phosphorylation leads to reduced appetite in response to fasting or cold exposure. At sub-thermoneutral temperatures, ACC DKI mice maintain normal energy expenditure and thermogenesis, but fail to increase appetite and lose weight. We demonstrate that the ACC DKI phenotype can be mimicked in wild type mice using a ghrelin receptor antagonist and that ACC DKI mice have impaired orexigenic responses to ghrelin, indicating ACC DKI mice have a ghrelin signaling defect. These data suggest that therapeutic strategies aimed at inhibiting ACC phosphorylation may suppress appetite following metabolic stress.
Laser Bead-on-Plate Welding and Overlap Seams for Increasing the Strength and Rigidity of High Strength Steel

Nowadays, high requirements are being placed on producing lighter automobiles with a higher strength. To achieve graded strength properties and to improve the rigidity of high strength thin steel sheets, the side effects of laser joining processes can be used. Local physical and geometrical effects which have previously only been observed as side effects can be purposefully used to increase the rigidity and strength of sheet metal structures. By using a focused laser beam with a diameter of several tenths of a millimetre, bead-on-plate and overlap welding seams have been produced. The energy needed to produce this kind of welding seam can be limited to a small area of the workpiece. In comparison to other procedures, the basic material characteristics are retained after welding, the main reason for this being localized heat input. The continual development of laser beam sources to provide higher output powers has extended their spectrum of use in the field of joining technologies. One aim of the research is to produce local physical and geometrical effects with two different laser systems, on the one hand, with an Nd:YAG laser with a maximum output power of 4 kW, and on the other hand with a Yb:YAG laser with an maximum output power of 3 kW. Bead-on-plate and overlap welding seams were produced to demonstrate that rigidity and strength can be increased in metal sheets. The investigations were carried out on two high strength steels H340LA with two different zinc coatings (D and ZE) and TRIP700. The sheets were tested using tensile tests, 3-point bending tests and fatigue strength tests. During the tests, metallographic analyses were carried out. Seven different specimens were investigated, one without bead-on-plate welding seams, and six with different welding geometries, expect the material H340LAZE which was investigated with three various welding geometries. To analyse the complex stress status, investigations of the residual stress and the fracture were done. Calculations of the seam volume were done to be able to calculate the tensile strength for linear bead-on-plate welding seams and the maximum force for bending overlap welding seams. The tensile and bending tests showed that higher forces were needed before failure occurred, e.g. bending the specimens. Furthermore, the investigations showed that the strength of the specimens with welding seams increased, in comparison to the specimens without welding seams. Another result of the experiments is that there is a dependency between the fatigue strength and the position of the seam relative to the direction of the testing force.
The Diverse Cellular and Animal Models to Decipher the Physiopathological Traits of Mycobacterium abscessus Infection

Mycobacterium abscessus represents an important respiratory pathogen among the rapidly-growing non-tuberculous mycobacteria. Infections caused by M. abscessus are increasingly found in cystic fibrosis (CF) patients and are often refractory to antibiotic therapy. The underlying immunopathological mechanisms of pathogenesis remain largely unknown. A major reason for the poor advances in M. abscessus research has been a lack of adequate models to study the acute and chronic stages of the disease leading to delayed progress of evaluation of therapeutic efficacy of potentially active antibiotics. However, the recent development of cellular models led to new insights in the interplay between M. abscessus with host macrophages as well as with amoebae, proposed to represent the environmental host and reservoir for non-tuberculous mycobacteria. The zebrafish embryo has also appeared as a useful alternative to more traditional models as it recapitulates the vertebrate immune system and, due to its optical transparency, allows a spatio-temporal visualization of the infection process in a living animal. More sophisticated immunocompromised mice have also been exploited recently to dissect the immune and inflammatory responses to M. abscessus. Herein, we will discuss the limitations, advantages and potential offered by these various models to study the pathophysiology of M. abscessus infection and to assess the preclinical efficacy of compounds active against this emerging human pathogen.
Urban Form Relationships with Walk Trip Frequency and Distance among Youth

Purpose. To assess the relationship among objectively measured urban form variables, age, and walking in youth. Design. Cross-sectional analyses of travel diary data mapped against urban form characteristics within a 1-km buffer of participant's place of residence. Setting. Youth in the Atlanta, Georgia region with selection stratified by income, household size, and residential density. Subjects. A total of 3161 5- to 20-year-olds who completed 2-day travel diaries. Diaries of those under 15 years were completed by a parent or legal guardian. Measures. Walking distances were calculated from a 2-day travel diary. Residential density, intersection density, land use mix, and commercial and recreation space were assessed within a 1-km network distance around residences. Analysis. Logistic regression analyses were performed for each urban form variable by age groups controlling for the demographic variables. All variables were then entered simultaneously into an analysis of the whole sample. Results. All five urban form variables tested were related to walking. Recreation space was the only variables associated with walking across the four different age groups. All the urban form variables were related to walking in the 12 to 15 years age cohort. For this group, the odds of walking were 3.7 times greater for those in highest- versus lowest-density tertile and 2.6 times greater for those with at least one commercial and 2.5 times greater for those with at least one recreational destination within 1 km from home. In the analysis of the full sample, number of cars, recreation space, and residential density were most strongly related to walking. Conclusions. Access to recreation or open space was the most important urban form variable related to walking for all age groups. Children aged 12 to 15 years old may be particularly influenced by urban form.
Smchd1 haploinsufficiency exacerbates the phenotype of a transgenic FSHD1 mouse model

In humans, a copy of the DUX4 retrogene is located in each unit of the D4Z4 macrosatellite repeat that normally comprises 8-100 units. The D4Z4 repeat has heterochromatic features and does not express DUX4 in somatic cells. Individuals with facioscapulohumeral muscular dystrophy (FSHD) have a partial failure of somatic DUX4 repression resulting in the presence of DUX4 protein in sporadic muscle nuclei. Somatic DUX4 derepression is caused by contraction of the D4Z4 repeat to 1-10 units (FSHD1) or by heterozygous mutations in genes responsible for maintaining the D4Z4 chromatin structure in a repressive state (FSHD2). One of the FSHD2 genes is the structural maintenance of chromosomes hinge domain 1 (SMCHD1) gene. SMCHD1 mutations have also been identified in FSHD1; patients carrying a contracted D4Z4 repeat and a SMCHD1 mutation are more severely affected than relatives with only a contracted repeat or a SMCHD1 mutation. To evaluate the modifier role of SMCHD1, we crossbred mice carrying a contracted D4Z4 repeat (D4Z4-2.5 mice) with mice that are haploinsufficient for Smchd1 (Smchd1MommeD1 mice). D4Z4-2.5/Smchd1MommeD1 mice presented with a significantly reduced body weight and developed skin lesions. The same skin lesions, albeit in a milder form, were also observed in D4Z4-2.5 mice, suggesting that reduced Smchd1 levels aggravate disease in the D4Z4-2.5 mouse model. Our study emphasizes the evolutionary conservation of the SMCHD1-dependent epigenetic regulation of the D4Z4 repeat array and further suggests that the D4Z4-2.5/Smchd1MommeD1 mouse model may be used to unravel the function of DUX4 in non-muscle tissues like the skin.
Epoxidized natural rubber-50 toughened polyamide 6 nanocomposites

Nanocomposites consisting of polyamide 6 (PA6) matrix with epoxidized natural rubber 50 (ENR-50) and organoclay-modified montmorillonite was prepared by melt blending in a twin-screw extruder followed by injection molding. The influence of varying amounts (0–30 phr) of ENR-50 loadings on ENR-50-toughened PA6 nanocomposites was examined. Morphological characterizations and mechanical and thermal properties of the blend and nanocomposites were investigated. Addition of ENR-50 resulted in a decrease in the tensile strength and modulus, while impact strength enhanced until a maximum at 10 wt% ENR-50. Thermal study revealed no significant change in the thermal properties with ENR-50 loadings. Exfoliated structure was observed using the x-ray diffraction patterns and was confirmed by transmission electron microscopic images. Scanning electron microscopic images revealed dispersed ENR-50 particles and increased rubber particles size with increasing ENR-50 loadings.
Carbon dioxide laser endoscopic posterior cordotomy technique for bilateral abductor vocal cord paralysis: a 15-year experience.

IMPORTANCE Treatment of bilateral vocal cord paralysis is a considerable challenge for otolaryngologists. Many surgical techniques have been developed for the management of this entity to eliminate the need for tracheotomy. OBJECTIVE To evaluate the success of the unilateral carbon dioxide laser endoscopic posterior cordotomy technique for bilateral abductor vocal cord paralysis. DESIGN A retrospective study. SETTING A university department of otolaryngology-head and neck surgery. PARTICIPANTS Sixty-six patients (58 women and 8 men) diagnosed as having bilateral abductor vocal cord paralysis. INTERVENTION Endoscopic posterior cordotomy with the carbon dioxide laser. MAIN OUTCOME MEASURES Decannulation and postoperative voice quality and exercise tolerance. RESULTS The most common etiologic factor was recurrent laryngeal nerve paralysis after thyroidectomy, observed in 61 patients (92%); an unknown cause was observed in 5 (8%). Unilateral cordotomy sufficed in 58 patients (88%). We performed revision procedures for vocal cord granuloma in 4 patients (6%). Bilateral cordotomy was required for 4 patients (6%) with an insufficient airway. Postoperative tracheotomy was needed for only 4 patients owing to the edema in the operation site. These patients underwent decannulation within a mean period of 7 days. No patient had poor postoperative exercise tolerance. We found no statistically significant difference between the preoperative and postoperative voice quality using the 10-item Turkish version of the Voice Handicap Index. CONCLUSIONS AND RELEVANCE Carbon dioxide laser endoscopic posterior cordotomy is a safe, minimally invasive, effective technique with a short operation time. A bilateral approach or a revision procedure is rarely required. Bilateral cordotomy should be reserved for patients with insufficient airway passage with unilateral cordotomy.
A Novel Batch Bayesian WIV Estimator for Three-Dimensional TMA Using Bearing and Elevation Measurements

In this paper, we derive a novel batch Bayesian weighted instrumental variable estimator for the three-dimensional (3D) target motion analysis (TMA) problem using bearing and elevation measurements. Unlike most existing 3D estimators based on instrumental variables, the proposed approach is able to incorporate a priori information into the estimation process and is proven to be approximately asymptotically unbiased. An approximate asymptotic covariance matrix is also presented to evaluate the performance of the estimator. Simulation results show that the proposed estimator outperforms its non-Bayesian counterpart and has an estimation performance on par with the conventional iterative maximum a posteriori estimator, while being orders of magnitude faster in a passive sonar TMA problem. Moreover, it was shown that the proposed approach can provide an accurate initialization for recursive estimators.
Clinical Results and Technical Complications of Posterior Implant‐Supported Modified Monolithic Zirconia Single Crowns and Short‐Span Fixed Dental Prostheses: A 2‐Year Pilot Study

PURPOSE The purpose of this clinical trial was to investigate the clinical outcomes of implant-supported modified monolithic zirconia single crowns (SCs) and short-span fixed dental prostheses (FDPs) after a follow-up of 2 years. MATERIALS AND METHODS Twenty-seven patients in need of implant-supported SCs or 3-unit FDPs in the posterior maxilla or mandible were consecutively selected for this study. A total of 56 modified monolithic zirconia prostheses including 44 SCs (8 screw-retained, 36 cement-retained) and twelve 3-unit FDPs (5 screw-retained, 7 cement-retained) were included in this study. All patients were followed at 6 months, 12 months, 18 months, and 2 years after placement of the modified monolithic zirconia prostheses. During the follow-up period, all prostheses were evaluated with clinical and radiographic examinations. The following technical parameters were assessed: framework fracture, fracture of veneering porcelain, screw loosening, loss of retention because of prosthesis de-cementation and opposing tooth fracture. RESULTS No implant was lost during the follow-up period, yielding a 2-year implant survival of 100%. One FDP failed because of framework fracture. The overall prosthesis survival rate was 98.2% after 2 years of clinical service. During the study, 5 complication events were observed in 3 SCs and one FDP, including one fracture of veneering porcelain and 2 screw loosenings in 3 SCs, and loss of retention and opposing tooth fracture in the same FDP. Therefore, the complication-free rate of prostheses was 91.1%. CONCLUSION According to the results of this study, the modified monolithic zirconia design used in this study resulted in a favorable short-term outcome for posterior implant-supported SCs and 3-unit FDPs.
Does self-help increase rates of help seeking for student mental health problems by minimizing stigma as a barrier?

ABSTRACT Objective: This study examined whether self-help (books, websites, mobile apps) increases help seeking for mental health problems among college students by minimizing stigma as a barrier. Participants and Methods: A survey was conducted with 200 college students reporting elevated distress from February to April 2017. Results: Intentions to use self-help were low, but a significant portion of students unwilling to see mental health professionals intended to use self-help. Greater self-stigma related to lower intentions to seek professional help, but was unrelated to seeking self-help. Similarly, students who only used self-help in the past reported higher self-stigma than those who sought professional treatment in the past. Although stigma was not a barrier for self-help, alternate barriers were identified. Conclusions: Offering self-help may increase rates of students receiving help for mental health problems, possibly by offering an alternative for students unwilling to seek in-person therapy due to stigma concerns.
Effect of Temperature on Flavor Compounds and Sensory Characteristics of Maillard Reaction Products Derived from Mushroom Hydrolysate

Maillard reaction products (MRPs) were prepared from mushroom hydrolysate (MH) by heating with d-xylose and l-cysteine at various temperatures (100 °C–140 °C) for 2 h at a pH of 7.4. The sensory characteristics of MH and MRPs were evaluated by panelists and volatile compounds were analyzed by GC/MS. Additionally, partial least squares regression (PLSR) was performed to analyze the correlation between quantitative sensory characteristics and GC/MS data. GC/MS results revealed that higher reaction temperature resulted in more nitrogen and sulfur containing compounds in MRPs while alcohols, ketones and aldehydes were the major flavor compounds obtained in MH. PLSR results showed that 3-phenylfuran and 2-octylfuran were the compounds responsible for the caramel-like flavor; 1-octen-3-ol, (E)-2-octen-1-ol and geranyl acetone were significantly and positively correlated to mushroom-like flavor, whereas, 2-thiophene-carboxaldehyde, 2,5-thiophenedicarboxaldehyde and 3-methylbutanal positively affected MRPs meat-like attribute. Overall, 125 °C was identified as the optimal temperature for preparing MRPs with abundant volatile compounds and favorable sensory characteristics; the concentration of free amino acids and 5′-GMP, which are associated with the umami taste, in MRPs derived under 125 °C were 3 to 4 times higher than those in MH.
Quantitative optical lock‐in detection for quantitative imaging of switchable and non‐switchable components

Reversible photoswitching has been proposed as a way to identify molecules that are present in small numbers over a large, non‐switching, background. This approach, called optical‐lock‐in‐detection (OLID) requires the deterministic control of the fluorescence of a photochromic emitter through optical modulation between a bright (on) and a dark state (off). OLID yields a high‐contrast map where the switching molecules are pinpointed, but the fractional intensities of the emitters are not returned. The present work presents a modified OLID approach (quantitative OLID or qOLID) that yields quantitative information of the switching (fSW) and non‐switching (fNS) components. After the validation of the method with a sample dataset and image sequence, we apply qOLID to measurements in cells that transiently express the photochromic protein EYQ1. We show that qOLID is efficient in separating the modulated from the non‐modulated signal, the latter deriving from background/autofluorescence or fluorophores emitting in the same spectral region. Finally, we apply qOLID to Förster (Fluorescence) Resonance Energy Transfer (FRET) imaging. We here demonstrate that qOLID is able to highlight the distribution of FRET intensity in a sample by using a photochromic donor and a non‐photochromic acceptor.