Miracle-dz/UniFAR-FTUs huggingface dataset:
Increased presynaptic ATP levels coupled to synaptic activity at the crayfish neuromuscular junction

Levels of ATP and related adenylates were measured in the terminal region of efferent nerves in the crayfish opener muscle using the luciferin-luciferase method. Following 1 min of stimulation at 50 Hz, the average (+/- SE) ATP content rose from 13.4 (+/- 1.5) to 19.0 (+/- 2.1) nmol/mg dry weight. The amounts of ADP, AMP, and the phosphagen phosphoarginine did not change significantly. Thus, the increased ATP was not derived from any of these potential sources. The increase was found to depend on synaptic activation, however, for its magnitude was directly related to the concentration of extracellular Ca2+, and it was blocked when CoCl2, verapamil, ruthenium red, or gamma-methylglutamate and picrotoxin were added to the bath. Addition of ATP to the bath solution also increased nerve ATP levels. Based upon measurements of sucrose distribution, only 50% of this increase was in the extracellular water space. The remainder of the ATP had either entered the nerve, become adsorbed extracellularly, or both. Addition of 2- deoxy-D-glucose and gamma-32P-ATP to the bath resulted in the formation of 32P-2-deoxy-D-glucose-6-P by the nerve. This suggests that a fraction of the extracellular ATP does enter the neuron chemically intact. To determine whether exogenous ATP is the source of the increased ATP measured in the nerve following stimulation, the bath was assayed for ATP. Stimulation did cause ATP levels to increase significantly; however, the maximum concentration was 3 orders of magnitude lower than that required to increase ATP levels in resting nerve. (ABSTRACT TRUNCATED AT 250 WORDS)
Erinnern, Wiederholen Und Durcharbeiten

At its original appearance (which was at the end of 1914) the title of this paper ran: 'Weitere Ratsch1age zur Technik der Psychoanalyse (II): Erinnern, Wiederholen und Durcharbeiten. ' The title of the English translation of 1924, quoted above, is a rendering of this. From 1924 onwards the German editions adopted the shorter title. This paper is noteworthy, apart from its technical interest, for containing the first appearance of the concepts of the 'compulsion to repeat' (p. 150) and of 'working-through' (p. 155). REMEMBERING, REPEATING AND WORKING-THROUGH
Probing Steroidal Substrate Specificity of Cytochrome P450 BM3 Variants

M01A82W, M11A82W and M01A82WS72I are three cytochrome P450 BM3 (CYP102A1) variants. They can catalyze the hydroxylation of testosterone (TES) and norethisterone at different positions, thereby making them promising biocatalysts for steroid hydroxylation. With the aim of obtaining more hydroxylated steroid precursors it is necessary to probe the steroidal substrate diversity of these BM3 variants. Here, three purified BM3 variants were first incubated with eight steroids, including testosterone (TES), methyltestosterone (MT), cholesterol, β-sitosterol, dehydroepiandrosterone (DHEA), diosgenin, pregnenolone and ergosterol. The results indicated that the two 3-keto-Δ4-steroids TES and MT can be hydroxylated at various positions by the three BM3 mutants, respectively. On the contrary, the three enzymes displayed no any activity toward the remaining six 3-hydroxy-Δ5-steroids. This result indicates that the BM3 mutants prefer 3-keto-Δ4-steroids as hydroxylation substrates. To further verify this notion, five other substrates, including two 3-hydroxy-Δ5-steroids and three 3-keto-Δ4-steroids, were carefully selected to incubate with the three BM3 variants. The results indicated the three 3-keto-Δ4-steroids can be metabolized to form hydroxysteroids by the three BM3 variants. On the other hand, the two 3-hydroxy-Δ5-steroids cannot be hydroxylated at any position by the BM3 mutants. These results further support the above conclusion, therefore demonstrating the 3-keto-Δ4–steroid substrate preference of BM3 mutants, and laying a foundation for microbial production of more hydroxylated steroid intermediates using BM3 variants.
Effects of Maintaining Hydration for On-Ice Decision-Making and Skill Execution in Experienced Male Ice Hockey Players.

EFFECTS OF MAINTAINING HYDRATION FOR ON-ICE DECISIONMAKING AND SKILL EXECUTION IN EXPERIENCED MALE HOCKEY PLAYERS. Gregory Sarkis Eskedjian Advisor: University of Guelph, 2015 Professor Lawrence L. Spriet This study investigated maintaining hydration with H2O, or a carbohydrateelectrolyte solution (CES) compared to mild dehydration by 1.5 – 2.0 % body mass (NF), on game-simulated hockey skills involving a decision-making component. During a 90min scrimmage, rate of perceived exertion was significantly higher in NF during the 2 period compared to CES and during the last half of the 3 period compared to H2O. During testing, CES and H2O had a faster average time during the weave agility test, a smaller increase in time between trials for reactive start skating sprint, and higher target hit percentage during the reactive passing and randomized shooting tests compared to NF. CES and H2O scored significantly better than NF during 5 of 8 hockey fatigue questionnaire responses, and all 4 subsections of the Profile of Mood States questionnaire. The results indicate that proper hydration can decrease perception of fatigue and help increase on-ice skill execution.
Learning a Recurrent Visual Representation for Image Caption Generation

In this paper we explore the bi-directional mapping between images and their sentence-based descriptions. We propose learning this mapping using a recurrent neural network. Unlike previous approaches that map both sentences and images to a common embedding, we enable the generation of novel sentences given an image. Using the same model, we can also reconstruct the visual features associated with an image given its visual description. We use a novel recurrent visual memory that automatically learns to remember long-term visual concepts to aid in both sentence generation and visual feature reconstruction. We evaluate our approach on several tasks. These include sentence generation, sentence retrieval and image retrieval. State-of-the-art results are shown for the task of generating novel image descriptions. When compared to human generated captions, our automatically generated captions are preferred by humans over $19.8\%$ of the time. Results are better than or comparable to state-of-the-art results on the image and sentence retrieval tasks for methods using similar visual features.
Integrin Mediated Adhesion of Osteoblasts to Connective Tissue Growth Factor (CTGF/CCN2) Induces Cytoskeleton Reorganization and Cell Differentiation

Pre-osteoblast adhesion and interaction with extracellular matrix (ECM) proteins through integrin receptors result in activation of signaling pathways regulating osteoblast differentiation. Connective tissue growth factor (CTGF/CCN2) is a matricellular protein secreted into the ECM. Prior studies in various cell types have shown that cell adhesion to CTGF via integrin receptors results in activation of specific signaling pathways that regulate cell functions, such as differentiation and cytoskeletal reorganization. To date, there are no studies that have examined whether CTGF can serve as an adhesive substrate for osteoblasts. In this study, we used the MC3T3-E1 cell line to demonstrate that CTGF serves as an adhesive matrix for osteoblasts. Anti-integrin blocking experiments and co-immunoprecipitation assays demonstrated that the integrin αvβ1 plays a key role in osteoblast adhesion to a CTGF matrix. Immunofluorescence staining of osteoblasts cultured on a CTGF matrix confirmed actin cytoskeletal reorganization, enhanced spreading, formation of focal adhesions, and activation of Rac1. Alkaline phosphatase (ALP) staining and activity assays, as well as Alizarin red staining demonstrated that osteoblast attachment to CTGF matrix enhanced maturation, bone nodule formation and matrix mineralization. To investigate whether the effect of CTGF on osteoblast differentiation involves integrin-mediated activation of specific signaling pathways, we performed Western blot, chromatin immunoprecipitation (ChIP) and qPCR assays. Osteoblasts cultured on a CTGF matrix showed increased total and phosphorylated (activated) forms of focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK). Inhibition of ERK blocked osteogenic differentiation in cells cultured on a CTGF matrix. There was an increase in runt-related transcription factor 2 (Runx2) binding to the osteocalcin gene promoter, and in the expression of osteogenic markers regulated by Runx2. Collectively, the results of this study are the first to demonstrate CTGF serves as a suitable matrix protein, enhancing osteoblast adhesion (via αvβ1 integrin) and promoting cell spreading via cytoskeletal reorganization and Rac1 activation. Furthermore, integrin-mediated activation of ERK signaling resulted in increased osteoblast differentiation accompanied by an increase in Runx2 binding to the osteocalcin promoter and in the expression of osteogenic markers.
Localization of electrodes in the subthalamic nucleus on magnetic resonance imaging.

OBJECT The authors describe a new method of localizing electrodes on magnetic resonance (MR) images and focus on the positions of both the most efficient contact and the electrode related to the MR imaging target. METHODS Thirty-one patients who had undergone bilateral subthalamic nucleus (STN) deep brain stimulation (DBS) were included in this study. Target coordinates were calculated in the anterior commissure-posterior commissure referential. A study of the correlation between the artifact and the related contact allowed one to deduce the contact position from the identification of the distal artifact on MR imaging. The best stimulation point corresponded with the contact resulting in the best Unified Parkinson's Disease Rating Scale (UPDRS) motor score improvement. It was compared (Student t-test) with the dorsal margin of the STN (DM STN), which was determined electrophysiologically. The distance between the target and the electrode was calculated individually in each axis. The best stimulation point was located at anteroposterior -2.34 +/- 1.63 mm, lateral 12.04 +/- 1.62 mm, and vertical -2.57 +/- 1.68 mm. This point was not significantly different from the DM STN (p < 0.05). The postoperative UPDRS motor score was 28.07 +/- 12.16, as opposed to the preoperative score of 46.27 +/- 13.89. The distance between the expected and actual target in the x- and y-axes was 1.34 +/- 1.02 and 1.03 +/- 0.76 mm, respectively. In the z-axis, 39.7% of the distal contacts were located proximal to the target. CONCLUSIONS This approach proposed for the localization of the electrodes on MR imaging shows that DBS is most effective in the dorsal and lateral part of the STN and indicates that the DBS electrode can be located more proximally than originally expected because of the caudal brain shift that may occur during the implantation procedure.
American Muslims: How the “American Creed” Fosters Assimilation and Pluralism

This article examines the status of American Muslims in the United States in relationship to other cultural groups and some of the widespread stereotypes that plague Muslims in contemporary society. Much has been written about the discrimination faced by Muslims, particularly after the September 11, 2001 attacks, spawned by religious, racial, and ethnic bigotry. Some polls show many Americans harbor some prejudices against Muslims, but these prejudices have not resulted in widespread violence or discrimination; although there has been some violence and discrimination experienced by some Muslims, the empirical data show that the majority of American Muslims are very successful economically, educationally, socially content, and politically active. This can be explained by America’s commitment to the “American Creed: The core values of the United States—liberty, democracy, equality of opportunity, the rule of law, individualism, separation of church and state, and social justice—have allowed Muslims, like other cultural groups, to assimilate into America’s political and economic culture and maintain their distinct religious identity. This demonstrates that America’s historical and painful struggle to evolve—defined as the attempt to narrow the gap between our ideals and actual behavior—has produced a more just and democratic, albeit imperfect, society.
The Utility of Mobile Eye Tracking Technology in the Forensic Analysis of Personal Injury

Early research on gaze patterns during human locomotion indicated that pedestrians tend to focus their gaze on the path 2 – 3 m ahead. More recent research in this area has utilized eye tracking technology to empirically assess eye movements during navigation in naturalistic environments. A similar approach to understanding personal injuries resulting in civil lawsuits has potential utility in the field of forensic human factors. The present study was conducted in the context of gathering empirical data to support expert opinions in a premises liability civil suit. Using a mobile head-mounted eye tracker, the gaze patterns of five individuals was assessed as they walked two paths covering the area where the plaintiff in the subject suit was injured. The results of this analysis indicated that, consistent with previous research, participants fixated the path ahead. Additionally, the duration of fixations on their final destination tended to be longer than fixations on other objects or areas in the forward view. The present study demonstrates the utility of empirical analysis of conditions surrounding a personal injury using eye tracking technology.
The Filipino immigration story: a selective literature review

Les AA. etudient l'immigration des philippins vers les Etats-Uni, montrent que ceux-ci ont rencontre les memes difficultes que d'autres populations dans la meme situation. Ils mettent en evidence le fait que leur niveau moyen de revenu est plus eleve que celui des afro-americains ou des latino-americains mais moins eleve que celui des autres populations asiatiques. La reussite scolaire des philippins est superieure a celle des blancs mais le taux d'abandon des etudes superieures est lui aussi eleve. Ils montrent que les sociologues doivent s'interesser aux groupes en situation de precarite ou de marginalite issus de cette communaute
One-nucleon effective generators of the Poincaré group derived from a field theory: Mass renormalization

We start from a Lagrangian describing scalar "nucleons" and mesons which interact through a simple vertex. Okubo's method of unitary transformation is used to describe a single nucleon dressed by its meson cloud. We find an expression for the physical mass of the nucleon being correct up to second order in the coupling constant. It is then verified that this result is the same as the corresponding expression found by Feynman techniques. Finally we also express the three boost operators in terms of the physical nucleon mass. Doing so we find expressions for all the ten generators of Poincar\'e transformations for the system of one single dressed nucleon.
Optimal link scheduling and channel assignment for convergecast in linear WirelessHART networks

Convergecast, in which data from a set of sources is routed toward one data sink, is a critical functionality for wireless networks deployed for industrial monitoring and control. We address the joint link scheduling and channel assignment problem for convergecast in networks operating according to the recent WirelessHART standard. For a linear network with N single-buffer devices, we demonstrate that the minimum time to complete convergecast is 2N-1 time-slots, and that the minimum number of channels required for this operation is ⌈N/2⌉. When the devices are allowed to buffer multiple packets, we prove that the optimal convergecast time remains the same while the number of required channels can be reduced to . For both cases, we present jointly time- and channel-optimal scheduling policies with complexity O(N2). Numerical results demonstrate that our schemes are also efficient in terms of memory utilization.
The Influence of Adolescent Communication Patterns to Emotional Intelligence and Learning Achievement of Senior High School Students in Bogor

The objectives of this research was to analyze the effects of adolescent communication pattern with family, school, and peers in relation to the emotional intelligence and learning achievement of High School students in Bogor. The research was conducted by survey method and analyzed using Structural Equation Modeling (SEM). The respondents were 372 students in second year of High School, consist of 206 female and 166 male students with age ranges of 15–18 years old. SEM analysis results showed that there was a significant influences between adolescent communication pattern with their family and school in relation to the emotional intelligence and learning achievement. The adolescent communication pattern with their peers had only significant effect on emotional intelligence and not significant on their learning achievement. The research also showed that emotional intelligence affected the learning achievement. -----------------------------------------------------------------------* Corresponding author. International Journal of Sciences: Basic and Applied Research (IJSBAR)(2016) Volume 30, No 1, pp 121-134 122
Combined diffusion-weighted and functional magnetic resonance imaging reveals a temporal-occipital network involved in auditory-visual object processing

Functional magnetic resonance imaging (MRI) showed that the superior temporal and occipital cortex are involved in multisensory integration. Probabilistic fiber tracking based on diffusion-weighted MRI suggests that multisensory processing is supported by white matter connections between auditory cortex and the temporal and occipital lobe. Here, we present a combined functional MRI and probabilistic fiber tracking study that reveals multisensory processing mechanisms that remained undetected by either technique alone. Ten healthy participants passively observed visually presented lip or body movements, heard speech or body action sounds, or were exposed to a combination of both. Bimodal stimulation engaged a temporal-occipital brain network including the multisensory superior temporal sulcus (msSTS), the lateral superior temporal gyrus (lSTG), and the extrastriate body area (EBA). A region-of-interest (ROI) analysis showed multisensory interactions (e.g., subadditive responses to bimodal compared to unimodal stimuli) in the msSTS, the lSTG, and the EBA region. Moreover, sounds elicited responses in the medial occipital cortex. Probabilistic tracking revealed white matter tracts between the auditory cortex and the medial occipital cortex, the inferior occipital cortex (IOC), and the superior temporal sulcus (STS). However, STS terminations of auditory cortex tracts showed limited overlap with the msSTS region. Instead, msSTS was connected to primary sensory regions via intermediate nodes in the temporal and occipital cortex. Similarly, the lSTG and EBA regions showed limited direct white matter connections but instead were connected via intermediate nodes. Our results suggest that multisensory processing in the STS is mediated by separate brain areas that form a distinct network in the lateral temporal and inferior occipital cortex.
Neuron-glia cross talk revealed in reverberating networks by simultaneous extracellular recording of spikes and astrocytes' glutamate transporter and K+ currents

In neocortex networks, we simultaneously captured spikes and the slower astrocytes' K+ and glutamate transporter (GluT) currents with the use of individual MEA electrodes. Inward and outward K+ currents in different regions of the glial syncytium suggested that spatial buffering was operant. Moreover, in organotypic slices from ventral tegmental area and prefrontal cortex, the large GluT current amplitudes allowed to measure transporter currents with a single electrode. Our method allows direct study of the dynamic interplay of different cell types in excitable and nonexcitable tissue.
Anesthetic Propofol Causes Glycogen Synthase Kinase-3&bgr;-regulated Lysosomal/Mitochondrial Apoptosis in Macrophages

Background: Overdose propofol treatment with a prolong time causes injury to multiple cell types; however, its molecular mechanisms remain unclear. Activation of glycogen synthase kinase (GSK)-3&bgr; is proapoptotic under death stimuli. The authors therefore hypothesize that propofol overdose induces macrophage apoptosis through GSK-3&bgr;. Methods: Phagocytic analysis by uptake of Staphylococcus aureus showed the effects of propofol overdose on murine macrophages RAW264.7 and BV2 and primary human neutrophils in vitro. The authors further investigated cell apoptosis in vitro and in vivo, lysosomal membrane permeabilization, and the loss of mitochondrial transmembrane potential (MTP) by propidium iodide, annexin V, acridine orange, and rhodamine 123 staining, respectively. Protein analysis identified activation of apoptotic signals, and pharmacologic inhibition and genetic knockdown using lentiviral-based short hairpin RNA were further used to clarify their roles. Results: A high dose of propofol caused phagocytic inhibition and apoptosis in vitro for 24 h (25 &mgr;g/ml, in triplicate) and in vivo for 6 h (10 mg/kg/h, n = 5 for each group). Propofol induced lysosomal membrane permeabilization and MTP loss while stabilizing MTP and inhibiting caspase protected cells from mitochondrial apoptosis. Lysosomal cathepsin B was required for propofol-induced lysosomal membrane permeabilization, MTP loss, and apoptosis. Propofol decreased antiapoptotic Bcl-2 family proteins and then caused proapoptotic Bcl-2-associated X protein (Bax) activation. Propofol-activated GSK-3&bgr; and inhibiting GSK-3&bgr; prevented Mcl-1 destabilization, MTP loss, and lysosomal/mitochondrial apoptosis. Forced expression of Mcl-1 prevented the apoptotic effects of propofol. Decreased Akt was important for GSK-3&bgr; activation caused by propofol. Conclusions: These results suggest an essential role of GSK-3&bgr; in propofol-induced lysosomal/mitochondrial apoptosis.
The Impact of Subject Age, Gender, and Arch Length on Attitudes of Syrian Dentists towards Shortened Dental Arches

Objective. This study aimed to investigate the impact of subject age, gender, and arch length on dentists' attitudes towards unrestored shortened dental arches. Materials and Methods. 93 Syrian dentists were interviewed and presented with 24 scenarios for male and female subjects of different ages and shortened dental arches of varying length. Participants were asked to indicate on a standardized visual analogue scale how they would value the health of the mouth if the posterior space was left unrestored. Results. A value of 0.0 represented the worst possible health state for a mouth and 1.0 represented the best. The highest mean value (0.73) was assigned to a shortened dental arch with missing second molar teeth in the mouth of a 70-year-old subject. A 35-year-old female subject with an extremely shortened dental arch (all molar and premolar teeth are missing) attracted the lowest mean value (0.26). The statistical analysis indicated a significant decrease in the value placed on unrestored shortened dental arches as the number of remaining teeth decreased (p < 0.008). While subject gender had almost no impact on dentists' attitudes towards shortened dental arches, the scenarios for the older shortened dental arch subjects attracted significantly higher values compared to the scenarios for the younger subjects (p < 0.017). Conclusion. Subject age and arch length affect dentists' attitudes towards shortened dental arches, but subject gender does not.