Miracle-dz/UniFAR-FTUs huggingface dataset:
GluR2 Deficiency Accelerates Motor Neuron Degeneration in a Mouse Model of Amyotrophic Lateral Sclerosis

AMPA receptor-mediated excitotoxicity has been implicated in the selective degeneration of motor neurons in amyotrophic lateral sclerosis (ALS). Motor neurons in vitro are particularly vulnerable to excessive AMPA receptor stimulation and one of the factors underlying this selective vulnerability is the presence of a large proportion of Ca2+-permeable (i.e. GluR2-lacking) AMPA receptors. However, the precise role of GluR2-lacking AMPA receptors in motor neuron degeneration remains to be defined. We therefore studied the impact of GluR2 deficiency on motor neuron death in vitro and in vivo. Cultured motor neurons from GluR2-deficient embryos displayed an increased Ca2+ influx through AMPA receptors and an increased vulnerability to AMPA receptor-mediated excitotoxicity. We deleted the GluR2 gene in mutant SOD1G93A mice by crossbreeding them with GluR2 knockout mice. GluR2 deficiency clearly accelerated the motor neuron degeneration and shortened the life span of mutant SOD1G93A mice. These findings indicate that GluR2 plays a pivotal role in the vulnerability of motor neurons in vitro and in vivo, and that therapies that limit Ca2+ entry through AMPA receptors might be beneficial in ALS patients.
Dupuytren’s contracture is associated with sprouting of substance P positive nerve fibres and infiltration by mast cells

Dupuytren’s contracture is a benign proliferative disease of connective tissue. It is characterised by an abnormal fibroblast proliferation and matrix deposition affecting the palmar fascia. There is evidence of genetic predisposition, but the precise cause remains unclear.1 Inflammatory infiltration of palmar tissue from Dupuytren’s contracture has been reported by several authors, who concluded that Dupuytren’s contracture may be a chronic inflammatory disease.2–4 To support the inflammatory hypothesis of Dupuytren’s contracture we characterised inflammatory elements such as nerve fibres and mast cells in palmar fascias affected by Dupuytren’s contracture. We compared 10 specimens from normal palmar fascia excised from subjects with idiopathic carpal tunnel syndrome (age 37–73 years) with 20 specimens from patients with fascias affected by Dupuytren’s contracture (age 48–78 years). Patients with diabetes or alcoholism were excluded from the study in order to avoid neuropathic alterations. Immunohistochemical stains were performed on paraffin embedded tissue. After antigen …
Interfacial Reactions between Polymer Derived SiC Fiber and Ti3Si(Al)C2

In this paper, interfacial reactions between polymer derived SiC fiber and Ti3Si(Al)C2 were analyzed. Strong interfacial reactions occurred in 20 vol.% SiCf/Ti3Si(Al)C2 composite during the vacuum spark plasma sintering at 1350 °C. At the begining, the Ti3Si(Al)C2 suffered spontaneous decomposition and resulted in the sublimation of gaseous Ti, Si and Al, and de-intercalation of TiC from the crystal structure, when it was combined with SiC fiber during sintering. Then, Ti reacted with the SiC phase and turbostratic graphite in the fiber and TiC was formed, and also Al was oxidized into Al2O3. Meanwhile, most of gaseous Si released from Ti3Si(Al)C2 escaped from the outer surfaces of the composite under the compressive stress of 30 MPa and the vacuum environment, the rest Si was cooled into solid state following gas-solid reaction mechanism.
Correlates of psychopathic personality traits in everyday life: results from a large community survey

Although the traits of psychopathic personality (psychopathy) have received extensive attention from researchers in forensic psychology, psychopathology, and personality psychology, the relations of these traits to aspects of everyday functioning are poorly understood. Using a large internet survey of members of the general population (N = 3388), we examined the association between psychopathic traits, as measured by a brief but well-validated self-report measure, and occupational choice, political orientation, religious affiliation, and geographical residence. Psychopathic traits, especially those linked to fearless dominance, were positively and moderately associated with holding leadership and management positions, as well as high-risk occupations. In addition, psychopathic traits were positively associated with political conservatism, lack of belief in God, and living in Europe as opposed to the United States, although the magnitudes of these statistical effects were generally small in magnitude. Our findings offer preliminary evidence that psychopathic personality traits display meaningful response penetration into daily functioning, and raise provocative questions for future research.
Single-Channel Properties Support a Potential Contribution of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels and If to Cardiac Arrhythmias

Background—The pacemaker current If is present in atrial and ventricular myocytes. However, it remains controversial whether If overexpression in diseased states might play a role for arrhythmogenesis, because first If activation in whole-cell recordings hardly overlapped the diastolic voltage of working myocardium. Methods and Results—To obtain further insight into IHCN and If properties, we provide for the first time detailed single-channel analysis of heterologously expressed hyperpolarization-activated cyclic nucleotide-gated (HCN) isoforms and native human If. HCN subtypes differed significantly in single-channel amplitude, conductance, and activation kinetics. Interestingly, threshold potentials of HCN isoforms were more positive than would have been expected from whole-cell measurements. Single-channel properties of cells cotransfected with HCN2 and HCN4 were distinct from cells expressing HCN2 or HCN4 alone, demonstrating that different HCN isoforms can influence current properties of a single HCN channel complex, thus providing direct functional evidence for HCN heteromerization. Pooled data of homomeric and heteromeric HCN channels and of native If extrapolated from maximum likelihood fits indicated a multistate gating scheme comprising 5 closed- and 4 open-channel states. Single-channel characteristics of If in human atrial myocytes closely resembled those of HCN4 or HCN2+HCN4, supporting the hypothesis that native If channels in atrial myocardium are heteromeric complexes composed of HCN4 and/or HCN2. Most interestingly, half-maximal activation of single-channel atrial If (−68.3±4.9 mV; k=−9.9±1.5; n=8) was well within the diastolic voltage range of human atrial myocardium. Conclusions—These observations support a potential contribution of HCN/If to the arrhythmogenesis of working myocardium under pathological conditions.
Angry Responses to Infant Challenges: Parent, Marital, and Child Genetic Factors Associated with Harsh Parenting Angry Responses to Infant Challenges: Parent, Marital, and Child Genetic Factors Associated with Harsh Parenting Nih Public Access Author Manuscript Methodological Challenges

(2015) Angry responses to infant challenges: parent, marital, and child genetic factors associated with harsh parenting. Child development, 86 This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published version. Copyright and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable, the material made available in SRO has been checked for eligibility before being made available. Copies of full text items generally can be reproduced, displayed or performed and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Abstract This study examined genetic and environmental influences on harsh parenting of 9-month-olds. We examined whether positive child-, parent-, and family-level characteristics were associated with harsh parenting in addition to negative characteristics. We were particularly interested in examining evocative gene-environment correlation (rGE) by testing the effect of birth parent temperament on adoptive parents' harsh parenting. Additionally, we examined associations among adoptive parents' own temperaments, their marital relationship quality, and harsh parenting. Adoptive fathers' (but not adoptive mothers') harsh parenting was inversely related to an index of birth mother positive temperament (reward dependence), indicating evocative rGE. Higher marital quality was associated with less harsh parenting, but only for adoptive fathers. Adoptive parents' negative temperamental characteristics (harm avoidance) were related to hostile parenting. Findings suggest the importance of enhancing positive family characteristics in addition to mitigating negative characteristics, as well as engaging multiple levels of the family system to prevent harsh parenting. Children have the potential to evoke strong positive and negative affective responses from parents, which then influence and organize caregiving behavior (Dix, 1991). All young children demonstrate challenging behaviors, such as prolonged crying that may be difficult to soothe, uncooperativeness with bathing or dressing, or difficulty with eating or sleeping. The degree to which parental negative emotion is evoked by these challenges and expressed in interactions with children is often characterized …
Dual implantation of a radio-telemeter and vascular access port allows repeated hemodynamic and pharmacological measures in conscious lean and obese rats

Expansion of physiological knowledge increasingly requires examination of processes in the normal, conscious state. The current study describes a novel approach combining surgical implantation of radio-telemeters with vascular access ports (VAPs) to allow repeated hemodynamic and pharmacological measures in conscious rats. Dual implantation was conducted on 16-week-old male lean and obese Zucker rats. Continued viability one month after surgery was observed in 67% of lean and 44% of obese animals, giving an overall 54% completion rate. Over the five-week measurement period, reliable and reproducible basal mean arterial pressure and heart rate measures were observed. VAP patency and receptor-independent vascular reactivity were confirmed by consistent hemodynamic responses to sodium nitroprusside (6.25 µg/kg). Acutely, minimal hemodynamic responses to repeated bolus administration of 0.2 mL saline indicated no significant effect of increased blood volume or administration stress, making repeated acute measures viable. Similarly, repeated administration of the β-adrenoceptor agonist dobutamine (30 µg/kg) at 10 min intervals resulted in reproducible hemodynamic changes in both lean and obese animals. Therefore, our study demonstrates that this new approach is viable for the acute and chronic assessment of hemodynamic and pharmacological responses in both lean and obese conscious rats. This technique reduces the demand for animal numbers and allows hemodynamic measures with minimal disruption to animals’ welfare, while providing reliable and reproducible results over several weeks. In conclusion, dual implantation of a radio-telemeter and VAP introduces a valuable technique for undertaking comprehensive studies involving repeated pharmacological tests in conscious animals to address important physiological questions.
Helium self-enrichment in globular clusters and the second parameter problem in M 3 and M 13

Inspection of the CM diagrams of globular clusters having similar heavy element content shows that the luminosity of the red giant bump relative to the turnoff (ΔV bump TO ) differs by more than 0.1 mag between clusters with different horizontal branch morphology. Unfortunately, careful consideration of the data leaves us with only one pair (M 3 and M 13) of clusters suitable for a quantitative discussion. For this pair we consider differences in age and helium content as possible causes for the difference in ΔV pump TO , and find more convincing support for the latter. A larger helium content in M 13 stars (Y ∼0.28 vs. Y ∼ 0.24) accounts for various CM diagram features, such as the difference in the luminosity level of RR Lyr variables and of the red giant bump with respect to the turnoff luminosity and the horizontal branch morphology. This enhanced helium can be tentatively understood in the framework of self-enrichment by massive asymptotic giant branch stars in the first ∼100 Myr of the cluster life. A modest self-enrichment can he present also in M 3 and can be the reason for the still unexplained presence of a not negligible number of luminous, Oosterhoff II type RR Lyr variables. The hypothesis that a larger helium content is the second parameter for clusters with very blue horizontal branch morphology could be checked by an accurate set of data for more clusters giving turnoff, RR Lyrs and bump magnitudes within a unique photometry.
Activation of a metabotropic glutamate receptor increases intracellular calcium concentrations in neurons of the avian cochlear nucleus

Metabotropic glutamate receptors have been shown to stimulate phosphatidylinositol metabolism, and subsequently liberate Ca2+ from intracellular stores, in a variety of tissue and cell types. We previously demonstrated that glutamate could stimulate phosphatidylinositol metabolism, generating inositol-1,4,5- trisphosphate (IP3), in isolated cochlear nucleus tissue from the chick. Using the calcium indicator dye fura-2 and ratiometric fluorescent imaging, this study examined the ability of glutamate and its analogs to liberate Ca2+ from intracellular stores of neurons of the avian cochlear nucleus, and qualitatively characterized the pharmacological profile of such an action. In normal, Ca(2+)-containing medium, glutamate, kainate (KA), alpha-amino-3-hydroxy-5-methyl- isoxazole-4-propionate (AMPA), NMDA, quisqualate (QUIS), and (+/-)- aminocyclopentane-trans-dicarboxylate (ACPD) elicited increases in intracellular calcium concentrations ([Ca2+]i). In the absence of external Ca2+, glutamate, quisqualate, and ACPD evoked increases in [Ca2+]i. In normal medium, the ionotropic glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the NMDA receptor antagonist 2-amino-5-phosphonovalerate (APV) attenuated but did not abolish the glutamate-evoked response and had no effect on the ACPD- evoked response. The putative metabotropic glutamate receptor antagonist 2-amino-3-phosphonopropionate (AP3) was without effect on the glutamate- and ACPD-evoked increases in [Ca2+]i in Ca(2+)-free medium. We conclude that a metabotropic glutamate receptor (mGluR) is present on cochlear nucleus neurons and is able to stimulate the phosphatidylinositol metabolism--Ca2+ signal transduction cascade.
Forgiveness or breakup: Sex differences in responses to a partner's infidelity

Infidelities--sexual, emotional, or both--afflict many long-term romantic relationships. When a person discovers a partner's betrayal, a major decision faced is to forgive the partner and remain together or to terminate the relationship. Because men and women have confronted different adaptive problems over evolutionary history associated with different forms of infidelity, we hypothesised the existence of sex differences in which aspects of infidelity would affect the likelihood of forgiveness or breakup. We tested this hypothesis using forced-choice dilemmas in which participants (N = 256) indicated how difficult it would be to forgive the partner and how likely they would be to break up with the partner, depending on the nature of the infidelity. Results support the hypothesis that men, relative to women: (a) find it more difficult to forgive a sexual infidelity than an emotional infidelity; and (b) are more likely to terminate a current relationship following a partner's sexual infidelity than an emotional infidelity. The Discussion provides directions for future work on the determinants of breakup and the psychology of forgiveness.
Value co-creation in service logic: A critical analysis

The underpinning logic of value co-creation in service logic is analysed. It is observed that some of the 10 foundational premises of the so-called service-dominant logic do not fully support an understanding of value creation and co-creation in a way that is meaningful for theoretical development and decision making in business and marketing practice. Without a thorough understanding of the interaction concept, the locus as well as nature and content of value co-creation cannot be identified. Value co-creation easily becomes a concept without substance. Based on the analysis in the present article, it is observed that the unique contribution of a service perspective on business (service logic) is not that customers always are co-creators of value, but rather that under certain circumstances the service provider gets opportunities to co-create value together with its customers. Finally, seven statements included in six of the foundational premises are reformulated accordingly.
Mineral Mapping Using Simulated Worldview-3 Short-Wave-Infrared Imagery

Abstract: WorldView commercial imaging satellites comprise a constellation developed by DigitalGlobe Inc. (Longmont, CO, USA). Worldview-3 (WV-3), currently planned for launch in 2014, will have 8 spectral bands in the Visible and Near-Infrared (VNIR), and an additional 8 bands in the Short-Wave-Infrared (SWIR); the approximately 1.0–2.5 μm spectral range. WV-3 will be the first commercial system with both high spatial resolution and multispectral SWIR capability. Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) data collected at 3 m spatial resolution with 86 SWIR bands having 10 nm spectral resolution were used to simulate the new WV-3 SWIR data. AVIRIS data were converted to reflectance, geographically registered, and resized to the proposed 3.7 and 7.5 m spatial resolutions. WV-3 SWIR band pass functions were used to spectrally resample the data to the proposed 8 SWIR bands. Characteristic reflectance signatures extracted from the data for known mineral locations (endmembers) were used to map spatial locations of specific minerals. The WV-3 results, when compared to spectral mapping using the full AVIRIS SWIR dataset, illustrate that the WV-3 spectral bands should permit identification and mapping of some key minerals, however, minerals with similar spectral features may be confused and will not be mapped with the same detail as using hyperspectral systems. The high spatial resolution should provide detailed mapping of complex alteration mineral patterns not achievable by current multispectral systems. The WV-3 simulation results are promising and indicate that this sensor will be a significant tool for geologic remote sensing.
Enhanced Nodulation and Nitrogen Fixation in the Abscisic Acid Low-Sensitive Mutant enhanced nitrogen fixation1 of Lotus japonicus1[OA]

The phytohormone abscisic acid (ABA) is known to be a negative regulator of legume root nodule formation. By screening Lotus japonicus seedlings for survival on an agar medium containing 70 μm ABA, we obtained mutants that not only showed increased root nodule number but also enhanced nitrogen fixation. The mutant was designated enhanced nitrogen fixation1 (enf1) and was confirmed to be monogenic and incompletely dominant. The low sensitivity to ABA phenotype was thought to result from either a decrease in the concentration of the plant's endogenous ABA or from a disruption in ABA signaling. We determined that the endogenous ABA concentration of enf1 was lower than that of wild-type seedlings, and furthermore, when wild-type plants were treated with abamine, a specific inhibitor of 9-cis-epoxycarotenoid dioxygenase, which results in reduced ABA content, the nitrogen fixation activity of abamine-treated plants was elevated to the same levels as enf1. We also determined that production of nitric oxide in enf1 nodules was decreased. We conclude that endogenous ABA concentration not only regulates nodulation but also nitrogen fixation activity by decreasing nitric oxide production in nodules.
Effect of macroscale formation of intraluminal thrombus on blood flow in abdominal aortic aneurysms

A mathematical approach of blood flow within an abdominal aortic aneurysm (AAA) with intraluminal thrombus (ILT) is presented. The macroscale formation of ILT is modeled as a growing porous medium with variable porosity and permeability according to values proposed in the literature. The model outlines the effect of a porous ILT on blood flow in AAAs. The numerical solution is obtained by employing a structured computational mesh of an idealized fusiform AAA geometry and applying the Galerkin weighted residual method in generalized curvilinear coordinates. Results on velocity and pressure fields of independent cases with and without ILT are presented and discussed. The vortices that develop within the aneurysmal cavity are studied and visualized as ILT becomes more condensed. From a mechanistic point of view, the reduction of bulge pressure, as ILT is thickening, supports the observation that ILT could protect the AAA from a possible rupture. The model also predicts a relocation of the maximum pressure region toward the zone proximal to the neck of the aneurysm. However, other mechanisms, such as the gradual wall weakening that usually accompany AAA and ILT formation, which are not included in this study, may offset this effect.
Educational design and online support for an innovative project-based course in engineering design

A new course in Engineering Design and Innovation used a project-based learning approach to facilitate learning the design process, the development of design thinking and the skills required to solve open-ended design problems. The course involved over 950 first year students, in the Faculty of Engineering at the University of New South Wales. Students were enrolled in nine schools of engineering in the faculty. A WebCT Vista course was used to support student learning in design teams and to integrate and manage the course. Online facilitation methods were used to support student learning during several phases of the design process. Online peer assessment and review processes were used to encourage reflective learning and be time-efficient for academic staff. The paper includes survey data from the first offering of the course.
Design and Implementation of a Mobile Radiological Emergency Unit Integrated in a Radiation Monitoring Network

The first hours elapsed after a radiological incident are critical to take appropriate actions to protect the population and to assess its impact on the environment. The development of mobile laboratories equipped with different radiation detectors, with robust communication systems, and with a highly autonomous uninterruptible power supply constitutes the spearhead of modern radiological warning networks. Their main function is to provide additional radiation information with acceptable accuracy in the shortest possible time. This paper describes our development of a mobile laboratory and the demonstration of its usefulness in various emergency drills conducted in the vicinity of a nuclear power plant.