Miracle-dz/UniFAR-FTUs huggingface dataset:
Retrograde signaling in plants: from simple to complex scenarios

The concept of retrograde signaling posits that signals originating from chloroplasts or mitochondria modulate the expression of nuclear genes. A popular scenario assumes that signaling factors are generated in, and exported from the organelles, then traverse the cytosol, and act in the nucleus. In this scenario, which is probably over-simplistic, it is tacitly assumed that the signal is transferred by passive diffusion and consequently that changes in nuclear gene expression (NGE) directly reflect changes in the total cellular abundance of putative retrograde signaling factors. Here, this notion is critically discussed, in particular in light of an alternative scenario in which a signaling factor is actively exported from the organelle. In this scenario, NGE can be altered without altering the total concentration of the signaling molecule in the cell as a whole. Moreover, the active transport scenario would include an additional level of complexity, because the rate of the export of the signaling molecule has to be controlled by another signal, which might be considered as the real retrograde signal. Additional alternative scenarios for retrograde signaling pathways are presented, in which the signaling molecules generated in the organelle and the factors that trigger NGE are not necessarily identical. Finally, the diverse consequences of signal integration within the organelle or at the level of NGE are discussed. Overall, regulation of NGE at the nuclear level by independent retrograde signals appears to allow for more complex regulation of NGE than signal integration within the organelle.
Dopamine Modulates the Function of Group II and Group III Metabotropic Glutamate Receptors in the Substantia Nigra Pars Reticulata

Recent findings have shown that dendritically released dopamine (DA) plays an important modulatory role in the substantia nigra pars reticulata (SNr). It is therefore possible that the loss of DA observed in Parkinson's disease (PD) could hold important consequences for nigral function. Previously, we have shown that activation of presynaptically localized group II metabotropic glutamate receptors (mGluRs) inhibits excitatory transmission at the subthalamic nucleus (STN)-SNr synapse and that activation of presynaptically localized group III mGluRs decreases excitatory and inhibitory transmission in the SNr. To test the hypothesis that nigral DA can modulate mGluR function in the SNr, we performed whole-cell patch-clamp recordings from γ-aminobutyric acidergic SNr neurons in slices obtained from rats that were acutely reserpinized. In slices obtained from reserpinized animals, the effect of group II mGluR activation by the selective agonist (+)-2-aminobicyclo[3·1·0]-hexane-2,6-dicarboxylate monohydrate (LY354740) (100 nM), but not group III mGluR activation [l-(+)-2-amino-4-phosphonobutyric acid, L-AP4, 500 μM], at STN-SNr synapses is significantly decreased. This effect could be mimicked in control slices by prior bath application of haloperidol (20 μM) andR-(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (SCH23390) (20 μM) but not sulpiride (50 μM). Furthermore, application of dopamine (100 μM) and (±)-6-chloro-7,8-dyhydroxy-3allyl-1-phenyl-2,3,4,5-tetra-hydro-1H-benzazepine (SKF82958) (1 μM) but not quinpirole (10 μM) could rescue the group II mGluR effect in reserpinized slices. The effect of group III mGluR activation (L-AP4, 100 μM) on inhibitory synaptic transmission was also significantly reduced in slices from reserpine-treated animals. This effect was mimicked by haloperidol (20 μM), SCH23390 (20 μM), and sulpiride (50 μM) in control slices. Thus, in a Parkinsonian state, the loss of nigral DA may add to the overall pathophysiological changes in basal ganglia output.
Affective Learning and Psychophysiological Reactivity in Dementia Patients

We examined the association of faces with biographical information that varied in emotional content in patients with Alzheimer's disease and a healthy control group. During two experimental sessions, participants rated neutral male faces on dimensions of hedonic valence and emotional arousal, later paired with fictitious biographical information. Both groups changed their ratings of the faces according to the biographical content. Free recall and recognition were tested in the second session. Patients neither recalled the biographical information nor recognized the faces, whereas the controls did. In addition, psychophysiological measures were taken in response to the face stimuli. Patients showed significant heart rate modulation as a function of their emotion ratings, whereas the controls did not. No correlation of rating changes with skin conductance was found in any group. Results suggest that psychophysiological reactions such as heart rate changes may indicate preserved affective associative learning in dementia patients despite impaired explicit memory.
In-Situ Formation of Reinforced Thermoplastic Composites

A process has been developed in our laboratories in which the reinforce ment of engineering thermoplastics is generated during melt processing. The process con sists of blending thermotropic liquid crystalline polymers with engineering thermoplastics in an extruder. On extruding these blends through a sheet die, films (or sheet) are formed in which the LCP phase exists in the form of thin fibrils. The formation of fibrils depends on the presence of certain hydrodynamic conditions defined by the viscosity ratio, capil lary number, and flow type. The sheets are then drawn to impart molecular orientation in the LCP phase. Polyphenylene sulfide (PPS) sheets reinforced with several different LCP's have been formed in which the properties are significantly enhanced. The modulus of these sheets represented almost a fourfold increase over that of the pure PPS sheet. The dynamic torsional properties of the sheet are also determined using a Rheometrics Mechanical Spectrometer and results are correlated with the structure and molecular ori entation.
Cerebral hemodynamics in CADASIL before and after acetazolamide challenge assessed with MRI bolus tracking.

BACKGROUND White matter lesions in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) are underlaid by severe ultrastructural changes of the arteriolar wall. Although chronic ischemia is presumed to cause the tissue lesions, the pattern of perfusion abnormalities and hemodynamic reserve in CADASIL, particularly within the white matter, remains unknown. METHODS We used the MRI bolus tracking method in 15 symptomatic patients with CADASIL (5 with dementia) and 10 age-matched control subjects before and 20 minutes after the intravenous injection of acetazolamide (ACZ, 17 mg/kg). Cerebral blood flow (CBF), blood volume (CBV), and mean transit time (MTT) were calculated both in the cortex and in the white matter according to the singular value decomposition technique. Perfusion parameters were obtained in regions of hyperintensities and within the normal-appearing white matter as observed on T2-weighted images. Analysis was performed with both absolute and relative (region/whole brain) values. RESULTS A significant reduction in absolute and relative CBF and CBV was found within areas of T2 hyperintensities in white matter in the absence of significant variations of MTT. This reduction was more severe in demented than in nondemented patients. No significant change in absolute CBF and CBV values was observed in the cortex of patients with CADASIL. A decrease in relative CBF and CBV values was detected in the occipital cortex. After ACZ administration, CBF and CBV increased significantly in both the cortex and white matter of affected subjects, but the increase in absolute CBF was lower within areas of increased signal on T2-weighted images in patients than in the white matter of control subjects. CONCLUSIONS In CADASIL, both basal perfusion and hemodynamic reserve are decreased in areas of T2 hyperintensities in the white matter. This hypoperfusion appears to be related to the clinical severity. The significant effect of ACZ on CBF and CBV suggests that cerebral perfusion might be increased using pharmacological vasodilation in CADASIL.
A non-coherent multi-user large scale SIMO system relaying on M-ary DPSK

A non-coherent detection assisted Differential Phase Shift Keying aided large-scale MIMO system is designed in a wireless uplink where multiple single-antenna users are transmitting to the base station's receiver equipped with a very large number of receive antennas. We show that the signal to interference plus noise ratio (SINR) scales with the number of receive antennas, which confirms the same scaling law found in coherent systems. We propose a range of constellation designs that allow us to separate the users' signals at the receiver by relying only on the knowledge of the average received power per user. We analyse the error probability and provide insights into the beneficial selection of the constellation parameters. Finally, we provide some numerical results showing that our proposals require a lower number of receive antennas to achieve a given error probability than other non-coherent benchmark schemes available in the literature, while they are not far from an equivalent coherent system relying on realistic channel estimation settings.
Natural climate solutions

Significance Most nations recently agreed to hold global average temperature rise to well below 2 °C. We examine how much climate mitigation nature can contribute to this goal with a comprehensive analysis of “natural climate solutions” (NCS): 20 conservation, restoration, and/or improved land management actions that increase carbon storage and/or avoid greenhouse gas emissions across global forests, wetlands, grasslands, and agricultural lands. We show that NCS can provide over one-third of the cost-effective climate mitigation needed between now and 2030 to stabilize warming to below 2 °C. Alongside aggressive fossil fuel emissions reductions, NCS offer a powerful set of options for nations to deliver on the Paris Climate Agreement while improving soil productivity, cleaning our air and water, and maintaining biodiversity. Better stewardship of land is needed to achieve the Paris Climate Agreement goal of holding warming to below 2 °C; however, confusion persists about the specific set of land stewardship options available and their mitigation potential. To address this, we identify and quantify “natural climate solutions” (NCS): 20 conservation, restoration, and improved land management actions that increase carbon storage and/or avoid greenhouse gas emissions across global forests, wetlands, grasslands, and agricultural lands. We find that the maximum potential of NCS—when constrained by food security, fiber security, and biodiversity conservation—is 23.8 petagrams of CO2 equivalent (PgCO2e) y−1 (95% CI 20.3–37.4). This is ≥30% higher than prior estimates, which did not include the full range of options and safeguards considered here. About half of this maximum (11.3 PgCO2e y−1) represents cost-effective climate mitigation, assuming the social cost of CO2 pollution is ≥100 USD MgCO2e−1 by 2030. Natural climate solutions can provide 37% of cost-effective CO2 mitigation needed through 2030 for a >66% chance of holding warming to below 2 °C. One-third of this cost-effective NCS mitigation can be delivered at or below 10 USD MgCO2−1. Most NCS actions—if effectively implemented—also offer water filtration, flood buffering, soil health, biodiversity habitat, and enhanced climate resilience. Work remains to better constrain uncertainty of NCS mitigation estimates. Nevertheless, existing knowledge reported here provides a robust basis for immediate global action to improve ecosystem stewardship as a major solution to climate change.
Linking Out-of-Body Experience and Self Processing to Mental Own-Body Imagery at the Temporoparietal Junction

The spatial unity of self and body is challenged by various philosophical considerations and several phenomena, perhaps most notoriously the “out-of-body experience” (OBE) during which one's visual perspective and one's self are experienced to have departed from their habitual position within one's body. Although researchers started examining isolated aspects of the self, the neurocognitive processes of OBEs have not been investigated experimentally to further our understanding of the self. With the use of evoked potential mapping, we show the selective activation of the temporoparietal junction (TPJ) at 330-400 ms after stimulus onset when healthy volunteers imagined themselves in the position and visual perspective that generally are reported by people experiencing spontaneous OBEs. Interference with the TPJ by transcranial magnetic stimulation (TMS) at this time impaired mental transformation of one's own body in healthy volunteers relative to TMS over a control site. No such TMS effect was observed for imagined spatial transformations of external objects, suggesting the selective implication of the TPJ in mental imagery of one's own body. Finally, in an epileptic patient with OBEs originating from the TPJ, we show partial activation of the seizure focus during mental transformations of her body and visual perspective mimicking her OBE perceptions. These results suggest that the TPJ is a crucial structure for the conscious experience of the normal self, mediating spatial unity of self and body, and also suggest that impaired processing at the TPJ may lead to pathological selves such as OBEs.
Identifying Dementia in the Primary Care Practice

Background: The purpose of this study was to evaluate the utility (i.e., positive and negative predictive value) of the 7 Minute Screen in identifying patients with probable Alzheimer's disease (AD) in a primary care practice. A second objective was to estimate the number of undiagnosed AD patients in a typical primary care practice. Methods: One hundred thirty-seven successive admissions (96%) of patients over the age of 60 to a primary care practice over a 53-day period who completed informed consent documents were administered the 7 Minute Screen. All patients who screened positive (n = 13) and a random sample of those who screened negative (n = 26) returned for full diagnostic evaluation. Positive predictive value (PPV) and negative predictive value (NPV) of the 7 Minute Screen were determined using the criterion standard of clinical diagnosis established by examination, history, and laboratory studies. Test-retest reliability and time for administration were also determined. Results: Of the 137 patients evaluated, 13 screened positive and 124 screened negative. Eleven of the 13 patients who screened positive were willing to return to the primary care practice for follow-up evaluation. A random sample of 26 patients who screened negative all agreed to return for follow-up evaluation. Of the 11 patients who screened positive who returned for evaluation, 10 were subsequently diagnosed with probable AD. The remaining patient was diagnosed with mixed dementia. The caregivers of the two patients who refused to return were contacted and both indicated that the patients were having significant cognitive problems as verified by an activities of daily living scale. Of the 26 patients who screened negative, 25 were judged to be cognitively normal and the 26th was judged to have mild cognitive impairment. Discussion: In successive admissions of patients over the age of 60 in a primary care practice, the 7 Minute Screen showed a PPV of 91% and an NPV of 96% in identifying patients who were subsequently identified with AD or other dementing disorder. These data suggest that this may be a useful instrument in identifying patients who should undergo diagnostic evaluation for AD and other dementing disorders. Additionally, extrapolation from the data in this practice suggests that there may be between 75 and 100 AD patients in the typical primary care practice, many of whom may not be diagnosed.
Change in Care Dependency and Nursing Care Problems in Nursing Home Residents with and without Dementia: A 2-Year Panel Study

Over time, chronic conditions like dementia can lead to care dependency and nursing care problems, often necessitating nursing home admission. This panel study (2012–2014) aims to explore changes in care dependency and nursing care problems (incontinence, malnutrition, decubitus, falls and restraints) in residents with and without dementia over time. In total, nine Austrian nursing homes participated, including 258 residents (178 with, 80 without dementia) who completed all five measurements. Data were collected with the International Prevalence Measurement of Care Problems questionnaire, the Care Dependency Scale and the Mini-Mental State Examination-2. Repeated measures ANOVA and crosstabs were used to analyse changes. The results showed that care dependency in dementia residents increased significantly for all 15 items of the Care Dependency Scale, with the highest increase being residents’ day-/night pattern, contact with others, sense of rules/values and communication. In contrast, care dependency in residents without dementia increased for four of the 15 items, with the highest increase being for continence, followed by getting (un)dressed. With respect to the assessed nursing care problems, residents with dementia and those without only differed significantly in terms of an increase in urinary- (12.3% vs. 14.2%), fecal- (17.4% vs. 10%), and double incontinence (16.7% vs. 11.9%). The results indicated that residents with dementia experienced increased care dependency in different areas than residents without dementia. Furthermore, residents with dementia experienced a lower increase in urinary incontinence but a higher increase in fecal- and double incontinence. These results help professionals to identify areas for improvement in dementia care.
MUTATION LOAD AND THE SURVIVAL OF SMALL POPULATIONS

Previous attempts to model the joint action of selection and mutation in finite populations have treated population size as being independent of the mutation load. However, the accumulation of deleterious mutations is expected to cause a gradual reduction in population size. Consequently, in small populations random genetic drift will progressively overpower selection making it easier to fix future mutations. This synergistic interaction, which we refer to as a mutational melt‐down, ultimately leads to population extinction. For many conditions, the coefficient of variation of extinction time is less than 0.1, and for species that reproduce by binary fission, the expected extinction time is quite insensitive to population carrying capacity. These results are consistent with observations that many cultures of ciliated protozoans and vertebrate fibroblasts have characteristic extinction times. The model also predicts that clonal lineages are unlikely to survive more than 104 to 105 generations, which is consistent with existing data on parthenogenetic animals. Contrary to the usual view that Muller's ratchet does more damage when selection is weak, we show that the mean extinction time declines as mutations become more deleterious. Although very small sexual populations, such as self‐fertilized lines, are subject to mutational meltdowns, recombination effectively eliminates the process when the effective population size exceeds a dozen or so. The concept of the effective mutation load is developed, and several procedures for estimating it are described. It is shown that this load can be reduced substantially when mutational effects are highly variable.
Treatment of acute ankle sprains: evidence on the use of an ankle brace is unclear

▸ Kemler E , van de Port I, Backx F, et al. A systematic review on the treatment of acute ankle sprain: brace versus other functional treatment types. Sports Med 2011; 41 :185–97. Ankle sprains are common, with an incidence of 60–70 per 10 000.1 Functional treatment (ie, brace, bandage or tape that allows some exercise or walking) appears more efficacious than cast immobilisation after ankle sprain,2 ,3 but the optimal type of functional treatment is unclear. A 2002 Cochrane systematic review reported a significantly shorter period in returning to work and sports if ankle braces rather than elastic bandages were used.3 However, this review included few studies and more studies are now available.4 The aim was to evaluate the effectiveness of ankle braces when compared with other functional treatment for acute ankle sprains. The search was conducted in four databases (PubMed, EMBASE, CINAHL and CENTRAL) and the reference lists of included studies. All randomised and quasi-randomised controlled trials, published between 1990 and April 2009, written in English, Dutch or …
THE 21 cm FOREST AS A PROBE OF THE REIONIZATION AND THE TEMPERATURE OF THE INTERGALACTIC MEDIUM

Using high-redshift radio sources as background, the 21 cm forest observations probe the neutral hydrogen absorption signatures of early structures along the lines of sight. Directly sensitive to the spin temperature of the hydrogen atoms, it complements the 21 cm tomography observations, and provides information on the temperature as well as the ionization state of the intergalactic medium (IGM). We use a radiative transfer simulation to investigate the 21 cm forest signals during the epoch of reionization. We first confirmed that the optical depth and equivalent width (EW) are good representations of the ionization and thermal state of the IGM. The features selected by their relative optical depth are excellent tracers of ionization fields, and the features selected by their absolute optical depth are very sensitive to the IGM temperature, so the IGM temperature information could potentially be extracted from 21 cm forest observation, thus breaking a degeneracy in 21 cm tomographic observation. With the EW statistics, we predict some observational consequences for 21 cm forest. From the distributions of EWs and the number evolution of absorbers and leakers with different EWs, we see clearly the cosmological evolution of ionization state of the IGM. The number density of potentially observable features decreases rapidly with increasing gas temperature. The sensitivity of the proposed EW statistic to the IGM temperature makes it a unique and potentially powerful probe of reionization. Missing small-scale structures, such as small filaments and minihalos that are unresolved in our current simulation, and lack of an accurate calculation of the IGM temperature, however, likely have rendered the presented signals quantitatively inaccurate. Finally, we discuss the requirements of the background radio sources for such observations, and find that signals with equivalent widths larger than 1 kHz are hopeful to be detected.