Miracle-dz/UniFAR-FTUs huggingface dataset:
Couple Play As A Predictor Of Couple Bonding, Physical Health And Emotional Health

Traditional couple counseling research focused on why people end relationships, with research only recently addressing what factors contribute to relationship satisfaction and stability. Yet, throughout this research, minimal attention has been paid to the role of play in couple counseling. The research available on play in couple counseling had varied definitions of couple play and was not based on current couple counseling theory. The research and anecdotal data on couple play proposed a strong relationship between couple play and the factors that predict successful, long-term couple relationships, individual physical health and emotional health. This study applied current couple counseling theory and research to define couple play and the relationship between couple play and couple bonding, physical health and emotional health. The hypotheses of the study were couple play would predict couple bonding; couple play would predict individual physical health; and couple play would predict individual emotional health. The results from a sample of 30 couples demonstrated couple play predicted measures of couple bonding, including relationship satisfaction, communication, conflict resolution, and the couple’s view of the relationship. Couple play demonstrated no relationship to individual physical or emotional health. Since couple play was predictive of successful, long-term couple relationship measures, the implications were discussed for using couple play in assessment and intervention in couple counseling and future research.
Long-Term Sphere Culture Cannot Maintain a High Ratio of Cancer Stem Cells: A Mathematical Model and Experiment

Acquiring abundant and high-purity cancer stem cells (CSCs) is an important prerequisite for CSC research. At present, researchers usually gain high-purity CSCs through flow cytometry sorting and expand them by short-term sphere culture. However, it is still uncertain whether we can amplify high-purity CSCs through long-term sphere culture. We have proposed a mathematical model using ordinary differential equations to derive the continuous variation of the CSC ratio in long-term sphere culture and estimated the model parameters based on a long-term sphere culture of MCF-7 stem cells. We found that the CSC ratio in long-term sphere culture presented as gradually decreased drift and might be stable at a lower level. Furthermore, we found that fitted model parameters could explain the main growth pattern of CSCs and differentiated cancer cells in long-term sphere culture.
Staying open to interpretation: engaging multiple meanings in design and evaluation

Human-Computer Interaction (HCI) often focuses on how designers can develop systems that convey a single, specific, clear interpretation of what they are for and how they should be used and experienced. New domains such as domestic and public environments, new influences from the arts and humanities, and new techniques in HCI itself are converging to suggest that multiple, potentially competing interpretations can fruitfully co-exist. In this paper, we lay out the contours of the new space opened by a focus on multiple interpretations, which may more fully address the complexity, dynamics and interplay of user, system, and designer interpretation. We document how design and evaluation strategies shift when we abandon the presumption that a specific, authoritative interpretation of the systems we build is necessary, possible or desirable.
Ocular ischemic syndrome – a systematic review

Summary Ocular ischemic syndrome is a rare condition, which is caused by ocular hypoperfusion due to stenosis or occlusion of the common or internal carotid arteries. Atherosclerosis is the major cause of changes in the carotid arteries. Ocular ischemic syndrome is manifested as visual loss, orbital pain and, frequently, changes of the visual field, and various anterior and posterior segment signs. Anterior segment signs include iris neovascularization and secondary neovascular glaucoma, iridocyclitis, asymmetric cataract, iris atrophy and sluggish reaction to light. Posterior eye segment changes are the most characteristic, such as narrowed retinal arteries, perifoveal telangiectasias, dilated retinal veins, mid-peripheral retinal hemorrhages, microaneurysms, neovascularization at the optic disk and in the retina, a cherry-red spot, cotton-wool spots, vitreous hemorrhage and normal-tension glaucoma. Differential diagnosis of ocular ischemic syndrome includes diabetic retinopathy and moderate central retinal vein occlusion. Carotid artery imaging and fundus fluorescein angiography help to establish the diagnosis of ocular ischemic syndrome. The treatment can be local, for example, ocular (conservative, laser and surgical) or systemic (conservative and surgical treatment of the carotid artery). Since the condition does not affect the eyes alone, patients with ocular ischemic syndrome should be referred for consultation to the neurologist, vascular surgeon and cardiologist.
Discussion: Enterochromaffin-Like Cell Pathobiology

The regulation of gastric acid secretion is a complex system, which has been elucidated over a number of years. The significance of the primary role of histamine as a stimulant of the parietal cell was proven conclusively by the demonstration of the efficacy of H2-receptor blockers [1]. The involvement of the vagal and neural system, primarily via muscarinic receptors, appears important, but its exact role in the secretion of acid remains to be determined fully. For some time, the role played by antral gastrin has been of primary interest to investigators of parietal cell function. While this role was for some considerable time proposed to be via direct action on the parietal cell, it has become obvious recently that this effect may be mediated predominantly via the ECL-cell system of the fundic mucosa. The biology of the enterochromaffin-like (ECL) cell has been described in considerable detail in this section. Recognition of its function and delineation of its secretory components have become a source of considerable interest to both physiologists and clinicians. A particular reason for this interest has been recognition of the fact that one of the consequences of long-term sustained acid inhibition is the generation of ECL hyperplasia [2]. In rodent species studied, this condition may develop from hyperplasia via a series of stages into neoplasia (carcinoid). Thus the question of ECL-cell hyperplasia has become an important biological issue and may be of some considerable clinical significance. The study of the interrelationship between the parietal cell and the ECL cell has thus become a focal issue in the area of gastric clinical and scientific endeavor. Although the ECL cell was first noted some 20 years ago, like many of the endocrine cells of the gut, no specific function was clearly definable. The identification of major amounts of histamine in the cell led to examination of the possibility that it might be the source of the histamine responsible for parietal cell stimulation [3]. Prior to this theory, it had been long held that the primary source of histamine in the gastric mucosa was chiefly from mast cells. Further examination of the question revealed that the ECL cell contained abundant histamine and histidine decarboxylase, and that stimulation by gastrin released amounts of histamine at levels capable of stimulating parietal cell secretion [4]. The resultant focus on the ECL cell as a primary regulator of gastric acid secretion and possibly even mucosal trophic regulation has resulted in a series of elegant studies in this area by a number of groups. To date, progress with the evaluation of this cell's function has, to a major extent, been limited by the unavailability of pure cell preparation. More recently, however, Prinz and his colleagues have generated a relatively pure ECL-cell preparation from rodents and been able to delineate certain aspects of the secretory functions of this cell [5]. Thus the ECL cell has been demonstrated to secrete histamine at physiological levels of gastrin stimulation and to respond to carbachol, forskolin, and epineph-
c‐jun N‐terminal kinase is involved in AUUUA‐mediated interleukin‐3 mRNA turnover in mast cells

Whereas signalling pathways involved in transcriptional control have been studied extensively, the pathways regulating mRNA turnover remain poorly understood. We are interested in the role of mRNA stability in cell activation and oncogenesis using PB‐3c mast cells as a model system. In these cells the short‐lived interleukin‐3 (IL‐3) mRNA is stabilized by ionomycin treatment and following oncogenesis. To identify the signalling pathways involved in these mechanisms, we analysed the effect of different kinase inhibitors. SB202190 and wortmannin were shown to antagonize ionomycin‐induced IL‐3 mRNA stabilization in PB‐3c cells in the presence of actinomycin D, and this effect coincided with their ability to inhibit c‐jun N‐terminal kinase (JNK) activation by ionomycin. Moreover, transfection of activated MEKK1 amplified ionomycin‐induced IL‐3 mRNA expression at the post‐transcriptional level, and a dominant‐negative mutant of JNK counteracted mRNA stabilization by ionomycin. Taken together, these data indicate that JNK is involved in the regulation of IL‐3 mRNA turnover in mast cells. In addition, transfection experiments revealed that the cis‐acting AU‐rich element in the 3′ untranslated region of IL‐3 mRNA is necessary and sufficient to confer JNK‐dependent mRNA stabilization in response to cell activation.
Na,K-ATPase Subunit b1 knock-in Prevents Lethality of b2 Deficiency in Mice

The b2 subunit of the Na,K-ATPase displays functional properties of both an integral constituent of an ion pump and an adhesion and neurite outgrowth-promoting molecule in vitro. To investigate whether the b1 subunit of the Na,K-ATPase can functionally substitute for the b2 isoform in vivo, we have generated b2/b1 knock-in mice by homologous recombination in embryonic stem cells. In b2/b1 knock-in mice, expression of b2 was abolished, whereas b1 mRNA expression from the mutated gene amounted to ;15% of the normal expression of b2 in the adult mouse brain and prevented the juvenile lethality observed for b2 null mutant mice. In contrast to b2 null mutant mice, the overall morphological structure of all analyzed brain regions was normal. By immunohistochemical analysis, b1 expression was detected in photoreceptor cells in the retina of knock-in mice at an age when expression of b1 and b2, respectively, is downregulated and persisting in the wild-type mice. Morphological analysis by light and electron microscopy revealed a progressive degeneration of photoreceptor cells. Apoptotic death of photoreceptor cells determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling analysis increased in b2/b1 knock-in mice with age. These observations suggest that the b1 subunit of the Na,K-ATPase can substitute sufficiently, at least in certain cell types, for the role of the b2 subunit as a component of a functional Na,K-ATPase, but they do not allow us to determine the possible role of the b2 subunit as an adhesion molecule in vivo.
DECONVOLUTION WITH UNKNOWN ERROR DISTRIBUTION

We consider the problem of estimating a density fX using a sample Y1, …, Yn from fY=fX⋆fe, where fe is an unknown density. We assume that an additional sample e1, …, em from fe is observed. Estimators of fX and its derivatives are constructed by using nonparametric estimators of fY and fe and by applying a spectral cut-off in the Fourier domain. We derive the rate of convergence of the estimators in case of a known and unknown error density fe, where it is assumed that fX satisfies a polynomial, logarithmic or general source condition. It is shown that the proposed estimators are asymptotically optimal in a minimax sense in the models with known or unknown error density, if the density fX belongs to a Sobolev space $H_{\mathcal{p}}$ and fe is ordinary smooth or supersmooth.
LUMOS - A Sensitive and Reliable Optode System for Measuring Dissolved Oxygen in the Nanomolar Range

Most commercially available optical oxygen sensors target the measuring range of 300 to 2 μmol L-1. However these are not suitable for investigating the nanomolar range which is relevant for many important environmental situations. We therefore developed a miniaturized phase fluorimeter based measurement system called the LUMOS (Luminescence Measuring Oxygen Sensor). It consists of a readout device and specialized “sensing chemistry” that relies on commercially available components. The sensor material is based on palladium(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorphenyl)-porphyrin embedded in a Hyflon AD 60 polymer matrix and has a KSV of 6.25 x 10-3 ppmv-1. The applicable measurement range is from 1000 nM down to a detection limit of 0.5 nM. A second sensor material based on the platinum(II) analogue of the porphyrin is spectrally compatible with the readout device and has a measurement range of 20 μM down to 10 nM. The LUMOS device is a dedicated system optimized for a high signal to noise ratio, but in principle any phase flourimeter can be adapted to act as a readout device for the highly sensitive and robust sensing chemistry. Vise versa, the LUMOS fluorimeter can be used for read out of less sensitive optical oxygen sensors based on the same or similar indicator dyes, for example for monitoring oxygen at physiological conditions. The presented sensor system exhibits lower noise, higher resolution and higher sensitivity than the electrochemical STOX sensor previously used to measure nanomolar oxygen concentrations. Oxygen contamination in common sample containers has been investigated and microbial or enzymatic oxygen consumption at nanomolar concentrations is presented.
Ischemic Preconditioning in the Liver Is Independent of Regulatory T Cell Activity

Ischemic preconditioning (IPC) protects organs from ischemia reperfusion injury (IRI) through unknown mechanisms. Effector T cell populations have been implicated in the pathogenesis of IRI, and T regulatory cells (Treg) have become a putative therapeutic target, with suggested involvement in IPC. We explored the role of Treg in hepatic IRI and IPC in detail. IPC significantly reduced injury following ischemia reperfusion insults. Treg were mobilized rapidly to the circulation and liver after IRI, but IPC did not further increase Treg numbers, nor was it associated with modulation of circulating pro-inflammatory chemokine or cytokine profiles. We used two techniques to deplete Treg from mice prior to IRI. Neither Treg depleted FoxP3.LuciDTR mice, nor wildtyoe mice depleted of Tregs with PC61, were more susceptible to IRI compared with controls. Despite successful enrichment of Treg in the liver, by adoptive transfer of both iTreg and nTreg or by in vivo expansion of Treg with IL-2/anti-IL-2 complexes, no protection against IRI was observed.We have explored the role of Treg in IRI and IPC using a variety of techniques to deplete and enrich them within both the liver and systemically. This work represents an important negative finding that Treg are not implicated in IPC and are unlikely to have translational potential in hepatic IRI.
Gas cooling within the diffuse ISM of late-type galaxies

We combine observations of spiral galaxies in the (CII) line at 158 m, made with the Long Wavelength Spectrometer aboard ISO, with previous data from the Kuiper Airborne Observatory to study the origin of this line, which is the main coolant of the interstellar medium at relatively low temperatures. We also use HI and CO(1 0) observations of these galaxies and estimate the respective line fluxes in the same beam as the (CII) observations. We conrm the existence of a linear relation between the (CII) line intensity and the CO(1 0) line intensity, that we extend to intrinsically fainter galaxies. The dispersion around this relation is signicant and due to variations in the far-UV flux, thus in the star formation rate. We nd that for the least active galaxies of our sample, in terms of star formation, the rate of (CII) line emission per interstellar hydrogen atom is similar to that in the Solar neighbourhood. For those galaxies, most of the (CII) line emission comes probably from the diuse cold atomic medium. In more active galaxies, considered globally, the average (CII) line emission is dominated by dense photodissociation regions and to some extent by the warm ionized diuse medium. This is true in the central regions of many spiral galaxies, and probably even in the interarm regions of the most actively star{forming ones.
The Presentation of Self in Everyday Life

hen an individual enters the presence of oth ers, they commonly seek to acquire information about him or to bring into play information about him already possessed. They will be interested in his general socio-economic status, his concep tion of self, his attitude toward them, his compe tence, his trustworthiness, etc. Although some of this information seems to be sought almost as an end in itself, there are usually quite practical reasons for acquiring it. Information about the individual helps to define the situation, enabling others to know in advance what he will expect of them and what they may expect of him. Informed in these ways, the others will know how best to act in order to call forth a desired response from him. For those present, many sources of information become accessible and many carriers (or “signvehicles”) become available for conveying this information. If unacquainted with the individual, observers can glean clues from his conduct and appearance which allow them to apply their previ ous experience with individuals roughly similar to the one before them or, more important, to apply untested stereotypes to him. They can also assume from past experience that only individuals of a par ticular kind are likely to be found in a given social setting. They can rely on what the individual says about himself or on documentary evidence he provides as to who and what he is. If they know, or know of, the individual by virtue of experience prior to the interaction, they can rely on assumptions as to the persistence and generality of psychological traits as a means of predicting his present and future behavior. However, during the period in which the indi vidual is in the immediate presence of the others, few events may occur which directly provide the others with the conclusive information they will need if they are to direct wisely their own activity . Many crucial facts lie beyond the time and place of interaction or lie concealed within it. For example, the “true” or “real” attitudes, beliefs, and emotions of the individual can be ascertained only indirectly , through his avowals or through what appears to be involuntary expressive behavior. Similarly , if the individual offers the others a product or service, they will often find that during the interaction there will be no time and place immediately available for eating the pudding that the proof can be found in. They will be forced to accept some events as con ventional or natural signs of something not directly available to the senses. In Ichheiser ’s terms, 1 the individual will have to act so that he intentionally or unintentionally expresses himself, and the others will in turn have to be impressed in some way by him. …
Galaxy And Mass Assembly (GAMA): galaxy close pairs, mergers and the future fate of stellar mass

We use a highly complete subset of the Galaxy And Mass Assembly II (GAMA-II) redshift sample to fully describe the stellar mass dependence of close pairs and mergers between 10 8 and 10 12 M� . Using the analytic form of this fit we investigate the total stellar mass accreting on tomoremassive galaxies across allmassratios.Depending onhow conservatively weselect our robust merging systems, the fraction of mass merging on to more massive companions is 2.0–5.6percent. Using the GAMA-II data we see no significant evidence for a change in the close pair fraction between redshift z = 0.05 and 0.2. However, we find a systematically higher fraction of galaxies in similar mass close pairs compared to published results over a similar redshift baseline. Using a compendium of data and the function γ M = A(1 + z) m to predict the major close pair fraction, we find fitting parameters of A = 0.021 ± 0.001 and m = 1.53 ± 0.08, which represents a higher low-redshift normalization and shallower power-law slope than recent literature values. We find that the relative importance of in situ star formation versus galaxy merging is inversely correlated, with star formation dominating the addition of stellar material below M ∗ and merger accretion events dominating beyond M ∗ . We find mergers have a measurable impact on the whole extent of the galaxy stellar mass function (GSMF), manifest as a deepening of the ‘dip’ in the GSMF over the next ∼Gyr and an increase in M ∗ by as much as 0.01–0.05 dex.
Coupled TCAD-SPICE simulation of parasitic BJT effect on SOI CMOS SRAM SEU

Single event upsets (SEU) produced by heavy ions in SOI CMOS SRAM cells were simulated using a mixed-mode approach, that is, two-dimensional semiconductor device simulation by TCAD tool coupled with circuit SPICE simulator. The effects of parasitic BJT and particle strike position on the SOI CMOS SRAM cells upset for transistor length scaling from 0.25 um to 65 nm are presented.
Time-Resolved Visualisation of Nearly-Native Influenza A Virus Progeny Ribonucleoproteins and Their Individual Components in Live Infected Cells

Influenza viruses are a global health concern because of the permanent threat of novel emerging strains potentially capable of causing pandemics. Viral ribonucleoproteins (vRNPs) containing genomic RNA segments, nucleoprotein oligomers, and the viral polymerase, play a central role in the viral replication cycle. Our knowledge about critical events such as vRNP assembly and interactions with other viral and cellular proteins is poor and could be substantially improved by time lapse imaging of the infected cells. However, such studies are limited by the difficulty to achieve live-cell compatible labeling of active vRNPs. Previously we designed the first unimpaired recombinant influenza WSN-PB2-GFP11 virus allowing fluorescent labeling of the PB2 subunit of the viral polymerase (Avilov et al., J.Virol. 2012). Here, we simultaneously labeled the viral PB2 protein using the above-mentioned strategy, and virus-encoded progeny RNPs through spontaneous incorporation of transiently expressed NP-mCherry fusion proteins during RNP assembly in live infected cells. This dual labeling enabled us to visualize progeny vRNPs throughout the infection cycle and to characterize independently the mobility, oligomerization status and interactions of vRNP components in the nuclei of live infected cells.
Source localization on solids using Kullback-Leibler discrimination information

We propose a new source localization algorithm for the development of a touch interface using surface-mounted sensors. This algorithm allows one to convert a hard surface into a touch pad. The proposed algorithm is based on triangulation using phase transit time-differences-of-arrival (TDOA) between the received signals from the sensors. In this inter-disciplinary research, we analyze the wave propagation of flexural vibration due to an impact on a surface which in turn allows one to gain insights to the frequency spectrum diversity of vibration signals and background noise. We then show how the Kullback-Leibler discrimination information can be used to determine the phase transit time of the vibration signal. The performance of the proposed algorithm is compared with existing TDOA approaches on real surfaces.