Miracle-dz/UniFAR-FTUs huggingface dataset:
Direct Evidence for Attention-Dependent Influences of the Frontal Eye-Fields on Feature-Responsive Visual Cortex

Voluntary selective attention can prioritize different features in a visual scene. The frontal eye-fields (FEF) are one potential source of such feature-specific top-down signals, but causal evidence for influences on visual cortex (as was shown for “spatial” attention) has remained elusive. Here, we show that transcranial magnetic stimulation (TMS) applied to right FEF increased the blood oxygen level–dependent (BOLD) signals in visual areas processing “target feature” but not in “distracter feature”–processing regions. TMS-induced BOLD signals increase in motion-responsive visual cortex (MT+) when motion was attended in a display with moving dots superimposed on face stimuli, but in face-responsive fusiform area (FFA) when faces were attended to. These TMS effects on BOLD signal in both regions were negatively related to performance (on the motion task), supporting the behavioral relevance of this pathway. Our findings provide new causal evidence for the human FEF in the control of nonspatial “feature”-based attention, mediated by dynamic influences on feature-specific visual cortex that vary with the currently attended property.
Intercalated chemotherapy and erlotinib for non-small cell lung cancer (NSCLC) with activating epidermal growth factor receptor (EGFR) mutations

ABSTRACT Among attempts to delay development of resistance to tyrosine kinase inhibitors (TKIs) in patients with advanced non-small cell lung cancer (NSCLC) with activating mutations of epidermal growth factor receptor (EGFR), intercalated therapy has not been properly evaluated. In a phase II trial, 38 patients with EGFR mutated NSCLC in advanced stage were treated with 4 to 6 3-weekly cycles of intercalated schedule with gemcitabine (1250 mg/m2, days 1 and 4), cisplatin (75 mg/m2, day 2) and erlotinib (150 mg, days 5 – 15), followed by continuous erlotinib as maintenance. In addition to standard radiologic evaluation according to RECIST, PET/CT was done prior to treatment and at 6 months, using PERCIST as a method for assessment of response. The primary endpoint was progression-free survival (PFS). In general, tolerance to treatment was good, even among 8 patients with performance status 2–3 and 13 patients with brain metastases; grade 4 toxicity included 2 cases of neutropenia and 4 thrombo-embolic events. Complete response (CR) or partial response (PR) were seen in 15 (39.5%) and 17 (44.7%) cases, respectively. All cases of CR were confirmed also by PET/CT. Median PFS was 23.4 months and median overall survival (OS) was 38.3 months. After a median follow-up of 35 months, 8 patients are still in CR and on maintenance erlotinib. In conclusion, intercalated treatment for treatment-naive patients with EGFR activating mutations leads to excellent response rate and prolonged PFS and survival. Comparison of the intercalated schedule to monotherapy with TKIs in a randomized trial is warranted.
Association of smoking status with prognosis in bladder cancer: A meta-analysis

There is considerable controversy regarding the association between smoking and prognosis in surgically treated bladder cancer. The present meta-analysis was performed to quantify the role of smoking status in bladder cancer recurrence, progression and patient survival by pooling the available previous data. Pubmed, Embase and the Cochrane Library databases were searched for eligible studies published prior to April 2016. Random and fixed effects models were used to calculate the summary relative risk estimates (SRRE). A total of 10,192 patients from 15 studies were included in the meta-analysis. There was evidence of positive associations between current smoking and the risk of recurrence (SRRE=1.23; 95% CI, 1.05–1.45) and mortality (SRRE=1.28; 95% CI, 1.07-1.52) in bladder cancer. Furthermore, former smoking had positive associations with bladder cancer recurrence (SRRE=1.22; 95% CI, 1.09-1.37) and mortality (SRRE=1.20; 95% CI, 1.03-1.41). However, there was no significant association between bladder cancer progression risk and current (SRRE=1.11; 95% CI, 0.71-1.75) or previous smoking (SRRE=1.16; 95% CI, 0.92-1.46). The findings indicate that current and former smoking increase the risk of recurrence and mortality in patients with bladder cancer. However, due to the nonrandomized and retrospective nature of the current study, patients may be prone to potential selection bias. Prospective and larger epidemiological studies with a longer follow-up are required to confirm these findings.
Expression of Collagen Genes in the Cones of Skin in the Duroc/Yorkshire Porcine Model of Fibroproliferative Scarring

During the past decades there has been minimal improvement in prevention and treatment of hypertrophic scarring. Reasons include the lack of a validated animal model, imprecise techniques to dissect scar into the histologic components, and limited methodology for measurement of gene expression. These problems have been addressed with the Duroc/Yorkshire model of healing, laser capture microdissection, and the Affymetrix Porcine GeneChip®. Here we compared collagen gene expression in fibroproliferative healing in the Duroc breed to nonfibroproliferative healing in the Yorkshires. We made shallow and deep dorsal wounds, biopsied at 1, 2, 3, 12, and 20 weeks. We sampled the dermal cones by laser capture microdissection, extracted and amplified the RNA, and hybridized Affymetrix Porcine GeneChips®. We also obtained samples of human hypertrophic scar approximately 20 weeks postinjury. Data were normalized and statistical analysis performed with mixed linear regression using the Bioconductor R/maanova package. Genes for further analysis were also restricted with four biologic criteria, including that the 20-week deep Duroc expression match the human samples. Eleven ollagen genes and seven collagen types were differentially over expressed in deep Duroc wounds including 1a1, 1a2, 3a1, 4a1, 4a2, 5a1, 5a2, 5a3, 6a3 (transcript variant 5), 14a1 and 15a1. COL7a1 gene was differentially under expressed in deep Duroc wounds. The results suggest that collagens I, III, IV, V, VI, VII, XIV, and XV1 are involved in the process of fibroproliferative scarring. With these clues, we will attempt to construct the regulatory pathway(s) of fibroproliferative healing.
Brain Structures Associated with Internet Addiction Tendency in Adolescent Online Game Players

With the development of the Internet, an increasing number of adolescents play online game excessively, which leads to adverse effects on individuals and society. Previous studies have demonstrated altered gray-matter volume (GMV) in individuals with Internet gaming disorder (IGD), but the relationship between the tendency to IGD and the GMV across whole brain is still unclear in adolescents. In the present study, anatomical imaging with high resolution was performed on 67 male adolescents who played online game; and Young’s Internet addiction test (IAT) was conducted to test the tendency to IGD. FMRIB Software Library (FSL) was used to calculate the voxel-based correlations between the GMV and the IAT score after controlling for the age and years of education. The GMVs of the bilateral postcentral gyri (postCG), the bilateral precentral gyri (preCG), the right precuneus, the left posterior midcingulate cortex (pMCC), the left inferior parietal lobe (IPL), and the right middle frontal gyrus (MFG) were negatively correlated with the IAT score. The correlation still existed between the IAT score and the GMVs of the bilateral postCG, the left preCG, the left pMCC, and the right MFG after controlling for the total time of playing online game. When the participants were divided into two groups according to the IAT score, the GMVs of these IAT-related brain regions were lower in high IAT score subgroup (IAT score >50) than in low IAT score subgroup (IAT score ≤50). Our results suggested that the GMVs of brain regions involved in sensorimotor process and cognitive control were associated with the IGD tendency. These findings may lead to new targets for preventing and treating the IGD.
Reliability of a single-session isokinetic and isometric strength measurement protocol in older men.

BACKGROUND The purposes of the current study were (a) to determine the test-retest reliability of a single-session isokinetic and isometric strength testing protocol in older healthy men, and (b) to compare the outcomes of the reliability measures derived from averaged torque scores with those derived from a single peak torque score. METHODS In 19 men (mean age, 72 +/- 5 years), both lower limbs were assessed independently on 2 separate test days using the Biodex System 3 dynamometer. After completing a 5-minute warm-up, each man performed three submaximal knee extensions followed by five maximal contractions at 90 degrees /s (CON), 0 degrees /s (ISO), and -90 degrees /s (ECC). Average (best 3 of 5) and peak CON, ISO, and ECC torque, and CON work and CON power were determined. Peak CON work and CON power were recorded from the highest peak torque concentric contraction (HPTCC). RESULTS Intraclass correlation coefficients ranging from 0.84 to 0.94 were found to have good reliability. The typical error as a coefficient of variation ranged from 8% to 10% for averaged measures and from 8% to 17% for peak torque and HPTCC. The ratio limits of agreement for average and peak CON, ISO, and ECC torque ranged from 23% to 33% and from 40% to 54% for average CON and HPTCC work and power. CONCLUSIONS The test-retest reliability of a single-session isokinetic and isometric strength testing protocol in this group of older healthy men displayed good relative reliability (intraclass correlation coefficient > 0.84); however, because the typical error as a coefficient of variation and ratio limits of agreement (absolute reliability) were large, single-session testing is not recommended.
Low-dose rituximab as an adjuvant therapy in pemphigus

Background: Pemphigus is a chronic autoimmune blistering disease where systemic steroids and immunosuppressants are the mainstay of therapy, but long-term treatment with these agents is associated with many side effects. Rituximab, a chimeric monoclonal anti-CD20 antibody, in low doses has shown efficacy as an adjuvant to reduce the dose of steroids. Aim: To study the clinical efficacy and safety of low-dose rituximab as an adjuvant therapy in pemphigus. Methods: Fifty patients with extensive pemphigus were selected, who either had recalcitrant pemphigus, were steroid dependent, had relapsed after pulse therapy, had anti-desmoglein levels >20, had contraindications to conventional treatment or wanted to avoid conventional treatment and its side effects. Two doses of rituximab (500 mg) were given 2 weeks apart and patients were regularly followed up every 2 weeks for 3 months and then monthly upto 2 years. Complete blood counts, liver function tests, renal function tests, skin biopsy, direct immunofluorescence and desmoglein levels were checked before and after rituximab administration. Pre-rituximab chest X-ray and electrocardiograph were also obtained. Results: At 3 months, 41 (82%) patients showed complete remission. Nine (18%) patients had partial remission. After 6–12 months, 20 (40% of enrolled patients) continued to be in remission and were off all systemic therapy and the remaining 19 (38%) were continuing to take low doses of steroids with or without other adjuvant immunosuppressants and 2 (4%) had to be given another 2 doses of rituximab and subsequently could be managed with low-dose steroids. Of the 9 patients in partial remission at 3 months, after 6–12 months 5 (10% of the total) were completely off treatment and went into complete remission and 4 (8%) were on additional treatment out of which 2 (4%) had to be given 2 additional doses of rituximab and were in partial remission with low-dose therapy at the end of 12 months. One patient developed urticaria as a side effect. Another developed herpes zoster. Conclusion: Our results show that low-dose rituximab is a well-tolerated and beneficial adjuvant therapy in recalcitrant pemphigus which helps reduce both the severity of disease as well as the dose of steroids and immunosuppressants.
Experimental measurements of partial discharge activity within LDPE/TiO2 nanocomposites

The presence of impurities, voids or gas bubbles during the manufacturing process of insulating materials of power cables leads to electrical degradation in the form of partial discharges (PDs). This paper aims to investigate the influence of incorporated titanium dioxide (TiO2) nanoparticles on the statistical parameters of PD activity within low density polyethylene (LDPE) matrix through using the solvent casting method. The surface morphology of synthesized LDPE/TiO2 nanocomposites was characterized by field emission scanning electron microscopy (FE-SEM). Then, the internal discharge measurements were performed using the traditional needle-plane configuration with the help of phase resolved partial discharge analyzer (PRPDA). This technique is used to analyze the statistical characteristics and feature of PDs with respect to the phase angle of applied voltage. It is found that the PD activity of all LDPE/TiO2 nanocomposite samples that represented by discharge magnitude, inception voltage, extinction voltage, and PD generation rate was enhanced compared to that of the pure LDPE. This was explained considering the effect of TiO2 nanoparticles in trapping process and initial electron generation inside the insulation material. Also, governing mechanisms for positive and negative cycle of PD activity were discussed.
Polymorphisms in four microRNAs and risk of oral squamous cell cancer: a meta-analysis

Objectives Single nucleotide polymorphisms in microRNAs (microRNA-196a2 rs11614913, microRNA-146a rs2910164, microRNA-149 rs2292832 and microRNA-499 rs3746444) have been inconsistently associated with risk for oral squamous cell cancer (OSCC). This meta-analysis aimed to assess the correlation between microRNA polymorphisms and susceptibility to OSCC. Materials and Methods Free words were used to search for the relevant studies without language limitations in electronic databases including PubMed, Embase, Web of Science and SCOPUS through June 15, 2017. Odds ratios (ORs) were calculated to investigate the effects of microRNA polymorphisms on oral cancer risk. Results Eleven studies were included. Analysis under the recessive model of microRNA-146a (CC vs GG+CG) showed significant differences (ORs = 0.874, P = 0.041). The G allele and the GG genotype of microRNA-499 were associated with OSCC risk (ORs >1, P < 0.05). MicroRNA-196a2 rs11614913 and microRNA-149 polymorphisms appeared to have no relationship with OSCC risk (P > 0.05). In the sensitivity analysis, there was a significant association between the TT genotype of microRNA-196a2 and OSCC risk (TT vs TC + CC, ORs < 1, P < 0.05). Conclusions There may be no significant relationship between microRNA-149 polymorphisms and OSCC risk, and the CC genotype of microRNA-146a may have protective effects against oral cancer. However, the G allele and the GG genotype of microRNA-499 may increase OSCC risk.
Study of electrical properties of biocomposites containing ferroelectric nanoparticles

Bio-ferroelectric composites represent an inexpensive and environmentally friendly electronic alternative for electrical applications such as capacitors, transistors, and actuators. The present research relates to the development of a biocomposite made of a chitosan–cellulose polymeric layer and bearing ferroelectric nanoparticles. The variables considered included the volume percentage of cellulose (15 v% and 25 v%) in the matrix and the amount of ferroelectric nanoparticles (0 wt.%, 10 wt.%, and 20 wt.%). Upon electrical characterization, the results indicated that the addition of the nanoparticles raised the capacitance and resistivity of the composite while the addition of cellulose lessened both electrical properties. The measured capacitance of the composites diminished as the applied voltage increased when contrasted with commercial capacitors where under similar testing conditions, as expected, the said capacity remained constant. Additionally, higher current flows were obtained for those capacitors than for a capacitor made with the nanocomposite. In general, it is proposed that capacitors made of this biopolymer reinforced with ferroelectric particles be suitable for radio frequency and microwave applications in which high electrical tunability and low dielectric loss are required.
On Information Metrics for Spatial Coding

The hippocampal formation is involved in navigation, and its neuronal activity exhibits a variety of spatial correlates (e.g., place cells, grid cells). The quantification of the information encoded by spikes has been standard procedure to identify which cells have spatial correlates. For place cells, most of the established metrics derive from Shannon’s mutual information (Shannon, 1948), and convey information rate in bits/sec or bits/spike (Skaggs et al., 1993; Skaggs et al., 1996). Despite their widespread use, the performance of these metrics in relation to the original mutual information metric has never been investigated. In this work, using simulated and real data, we find that the current information metrics correlate less with the accuracy of spatial decoding than the original mutual information metric. We also find that the top informative cells may differ among metrics, and show a surrogate-based normalization that yields comparable spatial information estimates. Since different information metrics may identify different neuronal populations, we discuss current and alternative definitions of spatially informative cells, which affect the metric choice.
In situ nano- to microscopic imaging and growth mechanism of electrochemical dissolution (e.g., corrosion) of a confined metal surface

Significance A surface forces apparatus was used with an electrochemical attachment to directly visualize electrochemical reactions, dissolution, and pitting on surfaces when confined in nanoscale gaps (or “crevices”) in real time. The results provide unique insights into how two closely apposed surfaces degrade under confinement, which is different from degradation of surfaces exposed to bulk solution. Degradation proceeds via local nucleation of “pits” at the periphery or center of the “contact area” that rapidly grow outward (in radius) and, more slowly, in depth, until the pits converge and the whole area has become one large pit. The results on a metal–inorganic interface are similar to previous observations on inorganic interfaces, and support a general mechanism of “pitting corrosion” at confined interfaces. Reactivity in confinement is central to a wide range of applications and systems, yet it is notoriously difficult to probe reactions in confined spaces in real time. Using a modified electrochemical surface forces apparatus (EC-SFA) on confined metallic surfaces, we observe in situ nano- to microscale dissolution and pit formation (qualitatively similar to previous observation on nonmetallic surfaces, e.g., silica) in well-defined geometries in environments relevant to corrosion processes. We follow “crevice corrosion” processes in real time in different pH-neutral NaCl solutions and applied surface potentials of nickel (vs. Ag|AgCl electrode in solution) for the mica–nickel confined interface of total area ∼0.03 mm2. The initial corrosion proceeds as self-catalyzed pitting, visualized by the sudden appearance of circular pits with uniform diameters of 6–7 μm and depth ∼2–3 nm. At concentrations above 10 mM NaCl, pitting is initiated at the outer rim of the confined zone, while below 10 mM NaCl, pitting is initiated inside the confined zone. We compare statistical analysis of growth kinetics and shape evolution of individual nanoscale deep pits with estimates from macroscopic experiments to study initial pit growth and propagation. Our data and experimental techniques reveal a mechanism that suggests initial corrosion results in formation of an aggressive interfacial electrolyte that rapidly accelerates pitting, similar to crack initiation and propagation within the confined area. These results support a general mechanism for nanoscale material degradation and dissolution (e.g., crevice corrosion) of polycrystalline nonnoble metals, alloys, and inorganic materials within confined interfaces.
Secondary Abdominal Compartment Syndrome in Children With Burns and Trauma: A Potentially Lethal Complication

Acute, rapid, and unimpeded increases in intra-abdominal pressure can lead to multiple organ dysfunction defined as the abdominal compartment syndrome (ACS). If this develops in the absence of obvious intra-abdominal injury, it has been termed secondary ACS (2° ACS). Massive fluid resuscitation in the presence of large burns or shock can lead to 2° ACS. The importance of early recognition and the need for urgent abdominal decompression have been recognized in adults; however, this has not been appreciated in the pediatric population. Medical records of four children diagnosed with 2°ACS were reviewed. Secondary ACS occurred in three children with burns and in one child with a traumatic brain injury. Three children underwent decompressive laparotomy, and one underwent successful percutaneous drainage. There were two survivors. Secondary ACS may be observed in burnt or traumatized children needing large volume resuscitation. Early recognition of 2° ACS by routine bladder pressure monitoring in this high-risk group of children may result in earlier decompression and a possible decrease in morbidity and mortality.
Insight into the evolution of microbial metabolism from the deep-branching bacterium, Thermovibrio ammonificans

Anaerobic thermophiles inhabit relic environments that resemble the early Earth. However, the lineage of these modern organisms co-evolved with our planet. Hence, these organisms carry both ancestral and acquired genes and serve as models to reconstruct early metabolism. Based on comparative genomic and proteomic analyses, we identified two distinct groups of genes in Thermovibrio ammonificans: the first codes for enzymes that do not require oxygen and use substrates of geothermal origin; the second appears to be a more recent acquisition, and may reflect adaptations to cope with the rise of oxygen on Earth. We propose that the ancestor of the Aquificae was originally a hydrogen oxidizing, sulfur reducing bacterium that used a hybrid pathway for CO2 fixation. With the gradual rise of oxygen in the atmosphere, more efficient terminal electron acceptors became available and this lineage acquired genes that increased its metabolic flexibility while retaining ancestral metabolic traits. DOI: http://dx.doi. org/10.7554/eLife.18990. 001