Miracle-dz/UniFAR-FTUs huggingface dataset:
Automating cross-layer diagnosis of enterprise wireless networks

Modern enterprise networks are of sufficient complexity that even simple faults can be difficult to diagnose - let alone transient outages or service degradations. Nowhere is this problem more apparent than in the 802.11-based wireless access networks now ubiquitous in the enterprise. In addition to the myriad complexities of the wired network, wireless networks face the additional challenges of shared spectrum, user mobility and authentication management. Not surprisingly, few organizations have the expertise, data or tools to decompose the underlying problems and interactions responsible for transient outages or performance degradations. In this paper, we present a set of modeling techniques for automatically characterizing the source of such problems. In particular, we focus on data transfer delays unique to 802.11 networks - media access dynamics and mobility management latency. Through a combination of measurement, inference and modeling we reconstruct sources of delay - from the physical layer to the transport - layer as well as the interactions among them. We demonstrate our approach using comprehensive traces of wireless activity in the UCSD Computer Science building.
Bacteriophage–Host Interactions and the Therapeutic Potential of Bacteriophages

Healthcare faces a major problem with the increased emergence of antimicrobial resistance due to over-prescribing antibiotics. Bacteriophages may provide a solution to the treatment of bacterial infections given their specificity. Enzymes such as endolysins, exolysins, endopeptidases, endosialidases, and depolymerases produced by phages interact with bacterial surfaces, cell wall components, and exopolysaccharides, and may even destroy biofilms. Enzymatic cleavage of the host cell envelope components exposes specific receptors required for phage adhesion. Gram-positive bacteria are susceptible to phage infiltration through their peptidoglycan, cell wall teichoic acid (WTA), lipoteichoic acids (LTAs), and flagella. In Gram-negative bacteria, lipopolysaccharides (LPSs), pili, and capsules serve as targets. Defense mechanisms used by bacteria differ and include physical barriers (e.g., capsules) or endogenous mechanisms such as clustered regularly interspaced palindromic repeat (CRISPR)-associated protein (Cas) systems. Phage proteins stimulate immune responses against specific pathogens and improve antibiotic susceptibility. This review discusses the attachment of phages to bacterial cells, the penetration of bacterial cells, the use of phages in the treatment of bacterial infections, and the limitations of phage therapy. The therapeutic potential of phage-derived proteins and the impact that genomically engineered phages may have in the treatment of infections are summarized.
Next-Generation Dengue Vaccines: Novel Strategies Currently Under Development

Dengue has become the most important arboviral infection worldwide with more than 30 million cases of dengue fever estimated to occur each year. The need for a dengue vaccine is great and several live attenuated dengue candidate vaccines are proceeding through clinical evaluation. The need to induce a balanced immune response against all four DENV serotypes with a single vaccine has been a challenge for dengue vaccine developers. A live attenuated DENV chimeric vaccine produced by Sanofi Pasteur has recently entered Phase III evaluation in numerous dengue-endemic regions of the world. Viral interference between serotypes contained in live vaccines has required up to three doses of the vaccine be given over a 12-month period of time. For this reason, novel DENV candidate vaccines are being developed with the goal of achieving a protective immune response with an immunization schedule that can be given over the course of a few months. These next-generation candidates include DNA vaccines, recombinant adenovirus vectored vaccines, alphavirus replicons, and sub-unit protein vaccines. Several of these novel candidates will be discussed.
Antisense transcriptional interference mediates condition-specific gene repression in budding yeast

Pervasive transcription generates many unstable non-coding transcripts in budding yeast. The transcription of such noncoding RNAs, in particular antisense RNAs (asRNAs), has been shown in a few examples to repress the expression of the associated mRNAs. Yet, such mechanism is not known to commonly contribute to the regulation of a given class of genes. Using a mutant context that stabilised pervasive transcripts, we observed that the least expressed mRNAs during the exponential phase were associated with high levels of asRNAs. These asRNAs also overlapped their corresponding gene promoters with a much higher frequency than average. Interrupting antisense transcription of a subset of genes corresponding to quiescence-enriched mRNAs restored their expression. The underlying mechanism acts in cis and involves several chromatin modifiers. Our results convey that transcription interference represses up to 30% of the 590 least expressed genes, which includes 163 genes with quiescence-enriched mRNAs. We also found that pervasive transcripts constitute a higher fraction of the transcriptome in quiescence relative to the exponential phase, consistent with gene expression itself playing an important role to suppress pervasive transcription. Accordingly, the HIS1 asRNA, normally only present in quiescence, is expressed in exponential phase upon HIS1 mRNA transcription interruption.
A Conceptual Framework on Factors Influencing Nurses’ Job Performance

The nurses' job performance can be regarded as a reflection of healthcare organizations, given that they comprise the largest group of human resource professionals who are responsible for providing high-quality care to patients. However, previous studies have emphasized that nurses in Malaysia are underperformed because of shortages in the workforce and excessive workload. Hence, understanding factors influencing the job performance of nurses will empower decision-makers to develop effective strategies aimed at enhancing nurses' performance. In Malaysia, only a few research have been performed on job performance, especially among nurses. Furthermore, limited research has delved into the specific factors influencing nurses’ job performance in Malaysia’s public hospital system. This study contributes to the existing knowledge by investigating the impact of spirituality at work and HRM practices on the nurses’ job performance in Malaysian public hospitals. This study employs a quantitative survey method using self-administered questionnaires, and the data collected will be analyzed using SPSS software Version 26. A total of 375 nurses from a population of 12, 514 nurses who worked at public hospitals in Malaysia will be selected as a sample of the study. This study offers potential benefits to authorities in Malaysian healthcare organizations by providing valuable insights for the development of compelling effective spirituality at work values and human resource management strategies aimed at enhancing the job performance of nurses.
Treatment results in advanced stage Hodgkin's lymphoma: A retrospective study

Background: Hodgkin′s lymphoma displays distinct epidemiological attributes in Asian population thus making it relevant to study whether there are any differences in treatment outcomes too when treated with current standard of care. Aim: To evaluate the treatment outcomes of de-novo advanced stage HL in adults. Materials and Methods: This retrospective study included de-novo advanced stage HL patients (≥15 years) registered at our center from January 2004 to December 2007. Treatment outcomes were measured in terms of response rates, overall survival (OS) and progression-free survival (PFS). Overall and PFS were calculated with Kaplan-Meier methodology and Cox-proportional hazards model was used for multivariate analysis to identify prognostic factors. Results: There were 125 patients (males 77%) who received minimum one cycle of chemotherapy with median age of 32 years (Range 15-65 years). Stage IV disease was seen in (46 patients) 37%; 75% (94 patients) patients had B symptoms. International prognostic score (IPS) ≤4 was seen in 95/112 (85%) patients. ABVD (adriamycin, bleomycin, vinblastine, dacarbazine) chemotherapy was given to 94%. Radiation to residual/bulky sites was given to 36% (45 patients). Response data was available for 112 patients; complete response in 76%; partial response in 10 % and progressive disease in 3 patients. Nineteen deaths (progressive disease-7, toxicity-8, unrelated cause-4) were observed. At median follow-up of 28 months, estimated 5-year OS and PFS were 60% and 58%, respectively. On multivariate analysis, IPS and response to treatment were significant factors for both OS and PFS. Conclusions: The treatment outcomes in this study are comparable with the published literature with limited follow-up data.
The Real-Time Publisher / Subscriber Inter-Process Communication Model for Distributed Real-Time Systems : Design and Implementation

Distributed real-time systems1 are becoming more pervasive in many domains including process control, discrete manufacturing, defense systems, air traffic control, and online monitoring systems in medicine. The construction of such systems, however, is impeded by the lack of simple yet powerful programming models and the lack of efficient, scalable, dependable and analyzable interfaces and their implementations. We argue that these issues need to be resolved with powerful application-level toolkits similar to that provided by ISIS [2]. In this paper, we consider the inter-process communication requirements which form a fundamental block in the construction of distributed realtime systems. We propose the real-time publisher/subscriber model, a variation of group-based programming and anonmyous communication techniques, as a model for distributed real-time inter-process communication which can address issues of programming ease, portability, scalability and analyzability. The model has been used successfully in building a software architecture for building upgradable real-time systems. We provide the programming interface, a detailed design and implementation details of this model along with some preliminary performance benchmarks. The results are encouraging in that the goals we seek look achievable.
Estimating Compressive and Shear Forces at L5-S1: Exploring the Effects of Load Weight, Asymmetry, and Height Using Optical and Inertial Motion Capture Systems

This study assesses the agreement of compressive and shear force estimates at the L5-S1 joint using inertial motion capture (IMC) within a musculoskeletal simulation model during manual lifting tasks, compared against a top-down optical motion capture (OMC)-based model. Thirty-six participants completed lifting and lowering tasks while wearing a modified Plug-in Gait marker set for the OMC and a full-body IMC set-up consisting of 17 sensors. The study focused on tasks with variable load weights, lifting heights, and trunk rotation angles. It was found that the IMC system consistently underestimated the compressive forces by an average of 34% (975.16 N) and the shear forces by 30% (291.77 N) compared with the OMC system. A critical observation was the discrepancy in joint angle measurements, particularly in trunk flexion, where the IMC-based model underestimated the angles by 10.92–11.19 degrees on average, with the extremes reaching up to 28 degrees. This underestimation was more pronounced in tasks involving greater flexion, notably impacting the force estimates. Additionally, this study highlights significant differences in the distance from the spine to the box during these tasks. On average, the IMC system showed an 8 cm shorter distance on the X axis and a 12–13 cm shorter distance on the Z axis during lifting and lowering, respectively, indicating a consistent underestimation of the segment length compared with the OMC system. These discrepancies in the joint angles and distances suggest potential limitations of the IMC system’s sensor placement and model scaling. The load weight emerged as the most significant factor affecting force estimates, particularly at lower lifting heights, which involved more pronounced flexion movements. This study concludes that while the IMC system offers utility in ergonomic assessments, sensor placement and anthropometric modeling accuracy enhancements are imperative for more reliable force and kinematic estimations in occupational settings.
Susceptibility of human Th17 cells to human immunodeficiency virus and their perturbation during infection.

BACKGROUND Identification of the Th17 T cell subset as important mediators of host defense and pathology prompted us to determine their susceptibility to human immunodeficiency virus (HIV) infection. METHODS AND RESULTS We found that a sizeable portion of Th17 cells express HIV coreceptor CCR5 and produce very low levels of CCR5 ligands macrophage inflammatory protein (MIP)-1alpha and MIP-1beta. Accordingly, CCR5(+) Th17 cells were efficiently infected with CCR5-tropic HIV and were depleted during viral replication in vitro. Remarkably, HIV-infected individuals receiving treatment had significantly reduced Th17 cell counts, compared with HIV-uninfected subjects, regardless of viral load or CD4 cell count, whereas treatment-naive subjects had normal levels. However, there was a preferential reduction in CCR5(+) T cells that were also CCR6 positive, which is expressed on all Th17 cells, compared with CCR6(-)CCR5(+) cells, in both treated and untreated HIV-infected subjects. This observation suggests preferential targeting of CCR6(+)CCR5(+) Th17 cells by CCR5-tropic viruses in vivo. Th17 cell levels also inversely correlated with activated CD4(+) T cells in HIV-infected individuals who are receiving treatment. CONCLUSIONS Our findings suggest a complex perturbation of Th17 subsets during the course of HIV disease potentially through both direct viral infection and virus indirect mechanisms, such as immune activation.
Role of soil quality in declining rooibos (Aspalathus linearis) tea yields in the Clanwilliam area, South Africa

Global demand for rooibos tea is increasing whereas yields are decreasing in the primary production area of Clanwilliam, South Africa. Commercial rooibos producers report that tea yields decline over time following the initial clearing of the natural fynbos veld. Therefore, the aim of this study was to investigate soil and plant quality in cultivated rooibos plantations of various ages (1–60 years) and adjacent, wild rooibos stands in pristine fynbos in the Clanwilliam area. Soil chemical and physical properties, plant total biomass, tea yields, foliar elemental contents, extent of root nitrogen (N) nodulation and mycorrhizal colonisation were assessed. The most prominent soil quality changes at the oldest cultivated sites compared with pristine fynbos soils were an increase in soil phosphorus (P) from 1.3–1.7 mg kg–1 to 4.0–17.0 mg kg–1, a general decline in total carbon from 0.14–0.29% to 0.09–0.10% and decline in total exchangeable basic cations from 0.64–0.78 cmolc kg–1 to 0.34–0.51 cmolc kg–1, and a concomitant increase in exchangeable aluminium (Al) from 0.10–0.30 cmolc kg–1 to 1.03–1.83 cmolc kg–1. Foliar N : P ratios notably declined at the cultivated sites (12 : 0 – 20 : 1) compared with pristine fynbos sites (27 : 1 – 33 : 1), indicating foliar P accumulation and lack of N. Soil P was strongly negatively correlated with rooibos root mycorrhizal colonisation. The decline in soil organic matter and basic cations, especially potassium (K), was most strongly correlated with the decline in rooibos shoot biomass yields at cultivated sites. These findings highlight the significant role of soil quality in declining yields of rooibos tea in the Clanwilliam area. Management practices should be implemented that increase soil organic matter and essential basic cations such as K, and soil P and exchangeable Al levels should be monitored.
Experimental Determination of Frequency of Laminar-Boundary Layer Instability Noise of NACA 0012

This paper presents simple method and new results of experimental investigation of laminar boundary-layer instability noise of an airfoil. The experimental method is based on small electret microphones, which are built in to the surface of the measured airfoil segment in pinholes. The paper discusses evaluation of the experimentally obtained data and presents new results for laminar boundary-layer vortex-shedding tonal sound frequency of NACA 0012. The main attribute of the proposed experimental method is the possibility of its usage in a non-aeroacoustic facility.
Cycling progenitors maintain epithelia while diverse cell types contribute to repair

It has recently been shown that stem and progenitor cells undergo population self‐renewal to maintain epithelial homeostasis. The fate of individual cells is stochastic but the production of proliferating and differentiating cells is balanced across the population. This new paradigm, originating in mouse epidermis and since extended to mouse oesophagus and mouse and Drosophila intestine, is in contrast to the long held model of epithelial maintenance by exclusively asymmetric division of stem cells. Recent lineage tracing studies have now shown that wound responses vary between tissues, and that a stem cell reserve is not essential as cycling progenitors and even differentiating cells contribute to regeneration.
Responses of Aspergillus flavus to Oxidative Stress Are Related to Fungal Development Regulator, Antioxidant Enzyme, and Secondary Metabolite Biosynthetic Gene Expression

The infection of maize and peanut with Aspergillus flavus and subsequent contamination with aflatoxin pose a threat to global food safety and human health, and is exacerbated by drought stress. Drought stress-responding compounds such as reactive oxygen species (ROS) are associated with fungal stress responsive signaling and secondary metabolite production, and can stimulate the production of aflatoxin by A. flavus in vitro. These secondary metabolites have been shown to possess diverse functions in soil-borne fungi including antibiosis, competitive inhibition of other microbes, and abiotic stress alleviation. Previously, we observed that isolates of A. flavus showed differences in oxidative stress tolerance which correlated with their aflatoxin production capabilities. In order to better understand these isolate-specific oxidative stress responses, we examined the transcriptional responses of field isolates of A. flavus with varying levels of aflatoxin production (NRRL3357, AF13, and Tox4) to H2O2-induced oxidative stress using an RNA sequencing approach. These isolates were cultured in an aflatoxin-production conducive medium amended with various levels of H2O2. Whole transcriptomes were sequenced using an Illumina HiSeq platform with an average of 40.43 million filtered paired-end reads generated for each sample. The obtained transcriptomes were then used for differential expression, gene ontology, pathway, and co-expression analyses. Isolates which produced higher levels of aflatoxin tended to exhibit fewer differentially expressed genes than isolates with lower levels of production. Genes found to be differentially expressed in response to increasing oxidative stress included antioxidant enzymes, primary metabolism components, antibiosis-related genes, and secondary metabolite biosynthetic components specifically for aflatoxin, aflatrem, and kojic acid. The expression of fungal development-related genes including aminobenzoate degradation genes and conidiation regulators were found to be regulated in response to increasing stress. Aflatoxin biosynthetic genes and antioxidant enzyme genes were also found to be co-expressed and highly correlated with fungal biomass under stress. This suggests that these secondary metabolites may be produced as part of coordinated oxidative stress responses in A. flavus along with antioxidant enzyme gene expression and developmental regulation.
On scheduling in map-reduce and flow-shops

The map-reduce paradigm is now standard in industry and academia for processing large-scale data. In this work, we formalize job scheduling in map-reduce as a novel generalization of the two-stage classical flexible flow shop (FFS) problem: instead of a single task at each stage, a job now consists of a set of tasks per stage. For this generalization, we consider the problem of minimizing the total flowtime and give an efficient 12-approximation in the offline setting and an online (1+µ)-speed O(1/µ2)-competitive algorithm. Motivated by map-reduce, we revisit the two-stage flow shop problem, where we give a dynamic program for minimizing the total flowtime when all jobs arrive at the same time. If there are fixed number of job-types the dynamic program yields a PTAS; it is also a QPTAS when the processing times of jobs are polynomially bounded. This gives the first improvement in approximation of flowtime for the two-stage flow shop problem since the trivial 2-approximation algorithm of Gonzalez and Sahni [29] in 1978, and the first known approximation for the FFS problem. We then consider the generalization of the two-stage FFS problem to the unrelated machines case, where we give an offline 6-approximation and an online (1+µ)-speed O(1/µ4)-competitive algorithm.
Ouabain binding to renal tubules of the rabbit

It is well known that ouabain, a specific inhibitor of Na-K ATPase-dependent transport, interferes with renal tubular salt reabsorption. In this study, we employed radiochemical methods to measure the kinetics of [3H]ouabain binding to slices of rabbit renal medulla and high resolution quantitative autoradiography to determine the location and number of cellular binding sites. The kinetics obeyed a simple bimolecular reaction with an association constant of 2.86 +/- 0.63 SD x 10(3) M-1 min-1 and a dissociation constant of 1.46 x 10(-3) min-1, yielding an equilibrium binding constant of 0.51 x 10(-6) M. Binding was highly dependent upon temperature. At a concentration of 10(-6) M, the rate of accumulation between 25 degrees C and 35 degrees C exhibited a Q10 of 1.8. At 0 degree C the rate of ouabain dissociation was negligible. The specificity of binding was demonstrated with increasing potassium concentrations. At a concentration of 1 microM, 6 mM, and 50 mM K+ produced a 2.5- and 7-fold decrease, respectively, in the rate of ouabain accumulation observed at zero K+. Binding was completely inhibited by 1 mM strophanthin K. The major site of ouabain binding was the thick ascending limb; little or no binding was observed in thin limbs and collecting ducts. Moreover, binding was confined to the basolateral membranes. From autoradiographic grain density measurements, it was estimated that each cell contains over 4 x 10(6) ouabain binding sites or Na-K ATPase molecules. These results taken together with physiological and biochemical observations suggest that Na-K ATPase plays a key role in salt reabsorption by this segment.
A flexible template for H.264/AVC block matching motion estimation architectures

Motion estimation architectures play a fundamental role in nowadays real time video encoding systems. However, in spite of their relevance, the influence of the allocation of the computing resources in terms of the final area, power dissipation and processing speed of such architectures has not been studied in depth in the recent literature. In this sense, a new approach for exploring different allocation alternatives of the computational resources within H.264/AVC block matching motion estimation architectures is presented in this paper. In particular, a novel architectural template is introduced allowing the establishment, in a highly flexible way, of different groups of processing elements (PEs) within one-dimensional motion estimation arrays. The use of this methodology has enabled the evaluation of different design tradeoffs, motivating the introduction of a new architecture composed of four independents groups with four PEs each, able to process CIF@60fps sequences with a reduced equivalent gate count and dynamic power savings.