Miracle-dz/UniFAR-FTUs huggingface dataset:
The structural basis for recognition of base J containing DNA by a novel DNA binding domain in JBP1

The J-binding protein 1 (JBP1) is essential for biosynthesis and maintenance of DNA base-J (β-d-glucosyl-hydroxymethyluracil). Base-J and JBP1 are confined to some pathogenic protozoa and are absent from higher eukaryotes, prokaryotes and viruses. We show that JBP1 recognizes J-containing DNA (J-DNA) through a 160-residue domain, DB-JBP1, with 10 000-fold preference over normal DNA. The crystal structure of DB-JBP1 revealed a helix-turn-helix variant fold, a ‘helical bouquet’ with a ‘ribbon’ helix encompassing the amino acids responsible for DNA binding. Mutation of a single residue (Asp525) in the ribbon helix abrogates specificity toward J-DNA. The same mutation renders JBP1 unable to rescue the targeted deletion of endogenous JBP1 genes in Leishmania and changes its distribution in the nucleus. Based on mutational analysis and hydrogen/deuterium-exchange mass-spectrometry data, a model of JBP1 bound to J-DNA was constructed and validated by small-angle X-ray scattering data. Our results open new possibilities for targeted prevention of J-DNA recognition as a therapeutic intervention for parasitic diseases.
Feedback generation of quantum Fock states by discrete QND measures

A feedback scheme for preparation of photon number states in a microwave cavity is proposed. Quantum Non Demolition (QND) measurement of the cavity field provides information on its actual state. The control consists in injecting into the cavity mode a microwave pulse adjusted to increase the population of the desired target photon number. In the ideal case (perfect cavity and measures), we present the feedback scheme and its detailed convergence proof through stochastic Lyapunov techniques based on super-martingales and other probabilistic arguments. Quantum Monte-Carlo simulations performed with experimental parameters illustrate convergence and robustness of such feedback scheme.
Gesture-based telemanipulation of a humanoid robot for home service tasks

Humanoid robots can be of great assistance to accomplish dexterous manipulation tasks for the impaired and elderly people, but diversity of the environments and complexity of autonomous algorithms still keep as challenging obstacles. In this paper, we proposed a gesture-based telemanipulation scheme to control the NAO humanoid robot. Taking advantage of the Leap Motion Controller, an intuitive and straightforward way for human-robot interaction has been realized. The NAO robot is telemanipulated to accomplish locomotion, dexterous manipulation and composite tasks, and validated by various experiments. This provides a promising technique for service robotics to deliver assistance for activities of daily living at home or in caring agencies, which could be of greatly help for the impaired and elderly people in an aging society.
Age-Associated Changes in the Spectral and Statistical Parameters of Surface Electromyogram of Tibialis Anterior

Age-related neuromuscular change of Tibialis Anterior (TA) is a leading cause of muscle strength decline among the elderly. This study has established the baseline for age-associated changes in sEMG of TA at different levels of voluntary contraction. We have investigated the use of Gaussianity and maximal power of the power spectral density (PSD) as suitable features to identify age-associated changes in the surface electromyogram (sEMG). Eighteen younger (20–30 years) and 18 older (60–85 years) cohorts completed two trials of isometric dorsiflexion at four different force levels between 10% and 50% of the maximal voluntary contraction. Gaussianity and maximal power of the PSD of sEMG were determined. Results show a significant increase in sEMG's maximal power of the PSD and Gaussianity with increase in force for both cohorts. It was also observed that older cohorts had higher maximal power of the PSD and lower Gaussianity. These age-related differences observed in the PSD and Gaussianity could be due to motor unit remodelling. This can be useful for noninvasive tracking of age-associated neuromuscular changes.
Hepatic Parenchymal Changes following Transcatheter Embolization and Chemoembolization in a Rabbit Tumor Model

Objective To compare the effects of transcatheter arterial chemoembolization (TACE) with transcatheter arterial embolization (TAE) on liver function, hepatic damage, and hepatic fibrogenesis in a rabbit tumor model. Materials and Methods Thirty-nine New Zealand white rabbits implanted with VX2 tumors in the left liver lobes were randomly divided into three groups: TAE, TACE, and control group. In the TAE group (n = 15), polyvinyl alcohol particles (PVAs) were used for left hepatic artery embolization. In the TACE group (n = 15), the tumors were treated with left hepatic arterial infusions of a suspension of 10-hydroxycamptothecin and lipiodol, followed by embolization with PVAs. In the control group (n = 9), the animals received sham treatment with distilled water. Serum and liver samples were collected at 6 hours, 3 days and 7 days after treatment. Liver damage was measured using a liver function test and histological analyses. Liver fibrogenesis and hepatic stellate cell (HSC) activation were evaluated using Sirius Red and anti-alpha-smooth muscle actin (α-SMA) immunohistochemical stains. Results TACE caused liver injury with greater increases in serum alanine aminotransferase and aspartate aminotransferase levels on day 3 (P<0.05). Histological analyses revealed increased hepatic necrosis in adjacent non-tumorous liver tissue from day 3 compared to the TAE group (Suzuki score of 2.33±1.29 versus 1.13±1.18, P = 0.001). HSC activation and proliferation were significantly increased in the TACE group compared to the control group at 3 and 7 days after treatment (0.074±0.014 vs. 0.010±0.006, and 0.088±0.023 vs. 0.017±0.009, P<0.05). Sirius Red staining demonstrated a statistically significant increase in collagen deposition in the livers in the TACE group 7 days after embolization compared to the control group (0.118±0.012 vs. 0.060±0.017, P = 0.05). Conclusion The results of this animal study revealed that TACE induced prominent hepatocellular damage and hepatic fibrogenesis, which compromised liver function and may be responsible for chronic liver decompensation.
Characterizing Tissue Fate After Transient Cerebral Ischemia of Varying Duration Using Quantitative Diffusion and Perfusion Imaging

Background and Purpose— The purpose of this study was to investigate the effects of reperfusion on ischemic lesion evolution and pixel-by-pixel apparent diffusion coefficient–cerebral blood flow (ADC-CBF) dynamics of core and mismatch tissues after 35, 60, and 95 minutes of transient focal ischemia in rats (n=28). Methods— Serial diffusion-, perfusion-, and T2-weighted imaging were performed up to 24 hours. The evolution of the magnetic resonance image-derived lesion volume was investigated and ADC-CBF scatterplots were performed to prospectively characterize the ADC and CBF dynamics of core and mismatch tissues with different fates. For comparison, similar analysis was performed on a historical 60-minute transient ischemia and permanent ischemia group. Results— ADC-derived lesions markedly decreased on reperfusion at 35 minutes to an average of 15±5% of prereperfusion lesion size (P<0.00001). At 24 hours, lesion volume as determined by T2 imaging increased again to 51±10% of prereperfusion lesion size. In the 95-minute group, ADC lesions only briefly decreased on reperfusion and then secondarily enlarged at 180 minutes, almost reaching prereperfusion lesion volume. Pixel-based analysis demonstrated that >85% of mismatch pixels were salvaged by reperfusion independent of ischemia duration. Recanalization at 35, 60, and 95 minutes resulted in recovery of 46%, 28%, and 9% of core pixels, respectively. Core and mismatch pixels that were ultimately salvaged had persistently higher (P<0.001) CBF values during ischemia in all reperfusion groups, associated with higher (P<0.05) ADC values. Conclusion— This study demonstrated substantial salvage of mismatch tissue after reperfusion independent of ischemia duration and substantial permanent recovery of initial core pixels with early reperfusion. Severity of CBF reduction during ischemia seems to be the main factor determining tissue fate.
Kualitas Hidup Pasien Skizofrenia Dipersepsikan Melalui Stigma Diri

Skizofrenia merupakan sekumpulan gejala atau sindrom yang dapat menyebabkan masalah kejiwaan yang sangat serius. Stigma diri muncul akibat efek negatif penilaian orang lain terhadap pasien Skizofrenia sehingga mengakibatkan penurunan kemampuan kerja, fungsi sosial, harga diri, dan harapan. Kualitas hidup pasien Skizofrenia erat kaitannya dengan disabilitas yang dialaminya berupa perubahan kognitif dan persepsi dalam menjalani kehidupan. Penelitian ini bertujuan untuk mengidentifikasi kualitas hidup pasien skizofrenia melalui stigma diri. Desain penelitian ini adalah descriptive corelative dengan pendekatan cross sectional, sampel 92 responden yang dipilih menggunakan teknik consecutive sampling . Analisis uji statistik menggunakan correlation test . Hasil dari penelitian adalah ada hubungan antara stigma diri dengan kualitas hidup pasien Skizofrenia dengan korelasi negatif (r= -0,568, p= 0,00). Level stigma diri termasuk kedalam klasifikasi stigma tinggi dan klasifikasi kualitas hidup yang rendah. Penelitian ini merekomen-dasikan pelaksanaan intervensi keperawatan yang berorientasi pada pasien berupa pencegahan stigma negatif dan peningkatan kualitas hidup. Abstract Quality of Life Schizophrenia Patients Treated Through Self Stigma . Schizophrenia is a syndrome that could induce seriously psychology problems. Self stigma can induce because of consequence of negative stereotype in patients with Schizophrenia with the result that diminished ability to work, social function, self-esteem and hope. Quality of life in patients with Schizophrenia related to disability because of impaired cognitive and perception in their life. This study was conducted to correlation between self stigmawith quality of life in patients Schizophrenia. Methodology use descriptive correlative with cross sectional study, used 92 patients with a diagnosis of Schizophrenia and used consecutive sampling. Statistical analysis test using correlation test. Result this study, there is significance correlation between self stigma and quality of life (p= 0.00 < 0.005) (r= -0.865). The level of self stigma of patients with schizophrenia was determined to be high, and the self stigma had a negative impact on the quality of life. This study recommends the implementation of patient oriented nursing interventions in the form of prevention of negative stigma and improvement of quality of life Keywords : internalized stigma, quality of life, Schizophrenia
Physical, Spectroscopic and Thermal Characterization of Biofield Treated Fish Peptone

The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. The fish peptone sample was divided into two parts; one part was subjected to Mr. Trivedi’s biofield treatment, coded as the treated sample and another part was coded as the control. The impact of biofield treatment was analysed through various analytical techniques and results were compared with the control sample. The particle size data revealed 4.61% increase in the average particle size (d50) along with 2.66% reduction in the surface area of the treated sample as compared to the control. The X-ray diffraction studies revealed the amorphous nature of the fish peptone sample; however no alteration was found in the diffractogram of the treated sample with respect to the control. The Fourier transform infrared studies showed the alterations in the frequency of peaks corresponding to N-H, C-H, C=O, C-N, and C-OH, functional groups in the treated sample as compared to the control. The differential scanning calorimetry data revealed the increase in transition enthalpy (ΔH) from -71.14 J/g (control) to -105.32 J/g in the treated sample. The thermal gravimetric analysis data showed the increase in maximum thermal degradation temperature (Tmax) from 213.31°C (control) to 221.38°C along with a reduction in the percent weight loss of the treated sample during the thermal degradation event. These data revealed the increase in thermal stability of the treated fish peptone and suggested that the biofield energy treatment may be used to improve the thermal stability of the heat sensitive compounds.
Pengaruh Integritas, Kompetensi, Objektivitas, Profesionalisme, Dan Due Professional Care Terhadap Kualitas Audit (Studi Empiris Persepsi Auditor pada KAP di Surakarta dan Yogyakarta)

The study aims to analyze the influence integrity, competence, objectivity, professionalism, and due pofessional care to the audit quality.Kind of this research is quantitative research. The population in this research islaboring auditor at Public Accountant Office in Surakarta and Yogyakarta. Determination of sample using convenience sampling and get 42 respondents. The data collected was primery data. Data analysis technique using double regresssion analysis. The result of this study indicate objectivity, professionlism, and due professional care have influence on the auditquality, while the integrity and competence has no influence on the auditquality.
Technology-enhanced learning in sports education using clickers : Satisfaction , performance and immediacy Vaso Constantinou and Andri Ioannou Cyprus

The article addresses ICT in Education by describing an empirical investigation of technologyenhanced sports education. The study examines the use of clickers by 162 Judo athletes during seminars on the rules and regulations of the sport. Results are based on quantitative data collected on athletes’ performances and attitudes and qualitative data from a focus group with the trainers. Findings suggest that the use of clickers is linked to athletes’ better satisfaction and performance and enables the feedback loop between athletes and trainers. More importantly, the implementation with the use of clickers can simulate the fast reaction time and decision making required during a Judo battle, which is difficult to achieve without the means of technology. Research of technology in the field of sport/physical education is limited. This study provides an interesting presentation of the potential of clickers to improve training quality for both athletes and trainers.
Behavioural Disturbances in a Temperate Fish Exposed to Sustained High-CO2 Levels

As atmospheric CO2 levels rise, the CO2 concentration in ocean surface waters increases through a process commonly referred to as ocean acidification. Recently, surprising behavioural modifications has been detected in the early life stages of tropical coral reef fish exposed to ocean acidification-relevant CO2 concentrations, but it has been unclear if this effect could occur in temperate waters. Here we show several severe behavioural disturbances, including effects on boldness, exploratory behaviour, lateralisation, and learning in a temperate fish, the three-spined stickleback (Gasterosteus aculeatus). The behavioural effects were consistent throughout the exposure period and increased in effect size with exposure time. We observed the effects on adult sticklebacks, a species known to be tolerant to other environmental stressors. Our findings suggest that behavioural abnormalities that stem from CO2 exposure are not restricted to sensitive tropical species or early life stages and may therefore affect fish on a global scale. The severity of disturbances and the possibility of a serious behavioural problem for fish across the globe is cause for concern.
Initial conditions for supersymmetric inflation

We perform a numerical investigation of the field evolution in the supersymmetric inflationary model based on radiative corrections. Supergravity corrections are also included. We find that, out of all the examined initial data, only about 10{percent} give an adequate amount of inflation and can be considered as {open_quotes}natural. {close_quotes} Moreover, these successful initial conditions appear scattered and more or less isolated. {copyright} {ital 1997} {ital The American Physical Society}
Dynamics of a coplanar-electrode plasma display panel cell. I. Basic operation

Plasma display panels (PDPs) are a technology for large-area high-brightness flat panel displays. There is considerable interest in improving PDP efficiency by optimizing the cell design, input voltage characteristics, operating conditions and gas mixture. In this article, we report on a two-dimensional computer model for PDPs which has been used to investigate the operation of a coplanar-electrode PDP cell sustained in He/Ne/Xe gas mixtures. The plasma transport equations are implicitly integrated in time to enable simulation of complex gas mixtures and PDP cell designs. To resolve the details of the electron dynamics, the electron temperature is computed by solving the electron energy equation. A Monte Carlo simulation for secondary electrons and a radiation transport model for visible light emission are also employed. The basic operation of the PDP cell is described in this article. The first pulse was usually found to initiate a discharge between the top electrodes and the bottom address electrode, wh.
Sea snakes rarely venture far from home

The extent to which populations are connected by dispersal influences all aspects of their biology and informs the spatial scale of optimal conservation strategies. Obtaining direct estimates of dispersal is challenging, particularly in marine systems, with studies typically relying on indirect approaches to evaluate connectivity. To overcome this challenge, we combine information from an eight-year mark-recapture study with high-resolution genetic data to demonstrate extremely low dispersal and restricted gene flow at small spatial scales for a large, potentially mobile marine vertebrate, the turtleheaded sea snake (Emydocephalus annulatus). Our mark-recapture study indicated that adjacent bays in New Caledonia (<1.15 km apart) contain virtually separate sea snake populations. Sea snakes could easily swim between bays but rarely do so. Of 817 recaptures of marked snakes, only two snakes had moved between bays. We genotyped 136 snakes for 11 polymorphic microsatellite loci and found statistically significant genetic divergence between the two bays (FST= 0.008, P < 0.01). Bayesian clustering analyses detected low mixed ancestry within bays and genetic relatedness coefficients were higher, on average, within than between bays. Our results indicate that turtleheaded sea snakes rarely venture far from home, which has strong implications for their ecology, evolution, and conservation.
Pengaruh Harapan Orang Tua dan Self-Efficacy Akademik terhadap Kecenderungan Fear of Failure pada Siswa: Analisis Perbandingan antara Siswa Kelas Unggulan dan Siswa Kelas Reguler

This study aims to examine the influence of parent expectation and academic self-efficacy on student’s fear of failure tendencies. In addition, this study also aims to examine the average comparison of parent expectation, academic self-efficacy, and student’s fear of failure tendencies between students of excellent class program and students of reguler class program. This study uses a quantitative approach with survey methods. The subjects of this study amounted to 271 students, 134 students of excellent class program and 137 students of reguler class program. The results showed that there was a significant influence of parent expectation and academic self-efficacy on student’s fear of failure tendencies, howeverthe results showed no significant differences the average comparison of parent expectation, academic self-efficacy, and student’s fear of failure tendencies between students of excellent class program and students of reguler class program
Responses of Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus to Simulated Food Processing Treatments, Determined Using Fluorescence-Activated Cell Sorting and Plate Counting

ABSTRACT Three common food pathogenic microorganisms were exposed to treatments simulating those used in food processing. Treated cell suspensions were then analyzed for reduction in growth by plate counting. Flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) were carried out on treated cells stained for membrane integrity (Syto 9/propidium iodide) or the presence of membrane potential [DiOC2(3)]. For each microbial species, representative cells from various subpopulations detected by FCM were sorted onto selective and nonselective agar and evaluated for growth and recovery rates. In general, treatments giving rise to the highest reductions in counts also had the greatest effects on cell membrane integrity and membrane potential. Overall, treatments that impacted cell membrane permeability did not necessarily have a comparable effect on membrane potential. In addition, some bacterial species with extensively damaged membranes, as detected by FCM, appeared to be able to replicate and grow after sorting. Growth of sorted cells from various subpopulations was not always reflected in plate counts, and in some cases the staining protocol may have rendered cells unculturable. Optimized FCM protocols generated a greater insight into the extent of the heterogeneous bacterial population responses to food control measures than did plate counts. This study underlined the requirement to use FACS to relate various cytometric profiles generated by various staining protocols with the ability of cells to grow on microbial agar plates. Such information is a prerequisite for more-widespread adoption of FCM as a routine microbiological analytical technique.
Improving RFID-based indoor positioning accuracy using Gaussian processes

The received signal strength (RSS) of radiofrequency signals emitted from beacons placed at known locations in an environment, can be used by a local positioning system (LPS) to estimate the location of a person or a mobile object. In indoor environments, interference, multipath propagation of RF signals, and the presence of obstacles and people, lead to a complex spatial distribution of the RSS, which is inaccurately described by simple parametric models. In this work, we present a Bayesian method for an indoor RFID location system which uses an observation model based in Gaussian processes (GPs) nonparametric regression to represent the environment-specific RSS distributions for the individual RFID tags. The experimental results in an indoor environment demonstrate the effectiveness of GPs in order to increase positioning accuracy.
Farnesoid X receptor associates with β-catenin and inhibits its activity in hepatocellular carcinoma

The association between the temporal activation of Wnt/β-catenin pathway and the spontaneous hepatocellular carcinoma (HCC) development in Farnesoid X receptor (FXR) knockout mice is not well understood. We found that Huh7 cells depleted with FXR by RNAi showed enhanced cell growth, migration and invasion in vitro and accelerated tumor xenografts formation in nude mice. And these phenotypes were attenuated by simultaneous knockdown of β-catenin with RNAi. Furthermore, we identified that FXR could bind with β-Catenin through AF1 domain, and disrupt the assembly of the core β-Catenin/TCF4 complex. Activation of FXR attenuated the DNA-binding activity of β-Catenin/TCF4, and subsequently, its targeting gene-cyclin D1 expression. Importantly, FXR expression was markedly reduced in human HCC, an event which correlated with aberrant activation of β-Catenin. These data identified FXR as a negative regulator of HCC development through direct suppression of Wnt/β-catenin pathway.