Miracle-dz/UniFAR-FTUs huggingface dataset:
Cervical cancer screening with HPV testing in the Valcamonica (Italy) screening programme

Objective We present the results of the first screening round and the first year of the second round of the Valcamonica Human Papillomavirus (HPV) pilot screening project. Setting From 2010 to 2012, the entire target female population (aged 25–64) was invited to the first HPV screening round in an area where Pap test screening had been active since 2002. Methods For HPV-negative women, the interval was three years. For HPV-positive women, a cytological smear was stained and interpreted. Positive cytologies were referred to colposcopy; negatives were referred to repeat HPV after one year. If HPV was persistently positive, women were referred to colposcopy; if negative, to normal screening. Results In 2010–12 18728 women were screened, slightly higher participation than with Pap test (18233 64.7%); 1633 were HPV-positive (8.7%); 843 were positive at cytology triage (referral rate at baseline 4.5%). Of those referred at the one year HPV test, 84% complied (660/780); 356 were persistently positive (1.9%). The total referral rate was 6.4% compared with 3.7% for the Pap test. The detection rate was 9.2/1000 compared with 5.0% for the Pap test. The HPV positivity rate during the second round in women previously negative was 3.9% and the detection rate in HPV-positive cytology-positive women was 0.8/1000. Conclusions HPV-based screening increases colposcopies at the first round, but also strongly increases the detection rate. At the second round, HPV prevalence was much lower and the detection rate also fell, corroborating the need for longer screening intervals in HPV-negative women.
Designing and evaluating touchless playful interaction for ASD children

Limited studies exist that explore motionbased touchless applications for children with ASD (Autism Spectrum Disorder) and investigate their design issues and the benefits they can bring to this target group. The paper reports a structured set of design guidelines that distill our experience gained from empirical studies and collaborations with therapeutic centers. These heuristics informed the design of three touchless games that were evaluated in a controlled study involving medium functioning ASD children at a therapeutic center. Our findings confirm the potential of motionbased touchless applications games for technologyenhanced interventions for this target group.
Quantitative analysis of the efficiency and mutagenic spectra of abasic lesion bypass catalyzed by human Y-family DNA polymerases

Higher eukaryotes encode various Y-family DNA polymerases to perform global DNA lesion bypass. To provide complete mutation spectra for abasic lesion bypass, we employed short oligonucleotide sequencing assays to determine the sequences of abasic lesion bypass products synthesized by human Y-family DNA polymerases eta (hPolη), iota (hPolι) and kappa (hPolκ). The fourth human Y-family DNA polymerase, Rev1, failed to generate full-length lesion bypass products after 3 h. The results indicate that hPolι generates mutations with a frequency from 10 to 80% during each nucleotide incorporation event. In contrast, hPolη is the least error prone, generating the fewest mutations in the vicinity of the abasic lesion and inserting dAMP with a frequency of 67% opposite the abasic site. While the error frequency of hPolκ is intermediate to those of hPolη and hPolι, hPolκ has the highest potential to create frameshift mutations opposite the abasic site. Moreover, the time (t50bypass) required to bypass 50% of the abasic lesions encountered by hPolη, hPolι and hPolκ was 4.6, 112 and 1 823 s, respectively. These t50bypass values indicate that, among the enzymes, hPolη has the highest abasic lesion bypass efficiency. Together, our data suggest that hPolη is best suited to perform abasic lesion bypass in vivo.
Tactile Responses in the Granule Cell Layer of Cerebellar Folium Crus IIa of Freely Behaving Rats

We recorded activity from the granule cell layer (GCL) of cerebellar folium Crus IIa as freely moving rats engaged in a variety of natural behaviors, including grooming, eating, and free tactile exploration. Multiunit responses in the 1000–4500 Hz range were found to be strongly correlated with tactile stimulation of lip and whisker (perioral) regions. These responses occurred regardless of whether the stimulus was externally or self-generated and during both active and passive touch. In contrast, perioral movements that did not tactually stimulate this region of the face (e.g., chewing) produced no detectable increases in GCL activity. In addition, GCL responses were not correlated with movement extremes. When rats used their lips actively for palpation and exploration, the tactile responses in the GCL were not detectably modulated by ongoing jaw movements. However, active palpation and exploratory behaviors did result in the largest and most continuous bursts of GCL activity: responses were on average 10% larger and 50% longer during palpation and exploration than during grooming or passive stimulation. Although activity levels differed between behaviors, the position and spatial extent of the peripheral receptive field was similar over all behaviors that resulted in tactile input. Overall, our data suggest that the 1000–4500 Hz multiunit responses in the Crus IIa GCL of awake rats are correlated with tactile input rather than with movement or any movement parameter and that these responses are likely to be of particular importance during the acquisition of sensory information by perioral structures.
Two closely‐related left‐right asymmetrically expressed genes, lefty‐1 and lefty‐2: their distinct expression domains, chromosomal linkage and direct neuralizing activity in Xenopus embryos

Vertebrates have numerous lateral asymmetries in the position of their organs, but the molecular basis for the determination of left–right (L‐R) asymmetries remains largely unknown. TGFβ‐related genes such as lefty and nodal are L‐R asymmetrically expressed in developing mouse embryos, and may be involved in L‐R determination.
Strong selection on mandible and nest features in a carpenter bee that nests in two sympatric host plants

Host plants are used by herbivorous insects as feeding or nesting resources. In wood-boring insects, host plants features may impose selective forces leading to phenotypic differentiation on traits related to nest construction. Carpenter bees build their nests in dead stems or dry twigs of shrubs and trees; thus, mandibles are essential for the nesting process, and the nest is required for egg laying and offspring survival. We explored the shape and intensity of natural selection on phenotypic variation on three size measures of the bees (intertegular width, wing length, and mandible area) and two nest architecture measures (tunnel length and diameter) on bees using the native species Chusquea quila (Poaceae), and the alloctonous species Rubus ulmifolius (Rosaceae), in central Chile. Our results showed significant and positive linear selection gradients for tunnel length on both hosts, indicating that bees building long nests have more offspring. Bees with broader mandibles show greater fitness on C. quila but not on R. ulmifolius. Considering that C. quila represents a selective force on mandible area, we hypothesized a high adaptive value of this trait, resulting in higher fitness values when nesting on this host, despite its wood is denser and hence more difficult to be bored.
Effect of process parameters settings and thickness on surface roughness of EBM produced Ti‐6Al‐4V

Purpose – Ti‐6Al‐4V is one of the most attractive materials being used in aerospace, automotive and medical implant industries. Electron beam melting (EBM) is one of the direct digital manufacturing methods to produce complex geometries of fully dense and near net shape parts. The EBM system provides an opportunity to built metallic objects with different processing parameter settings like beam current, scan speed, probe size on powder, etc. The purpose of this paper is to determine and understand the effect of part's thickness and variation in process parameter settings of the EBM system on surface roughness/topography of EBM fabricated Ti‐6Al‐4V metallic parts.Design/methodology/approach – A mathematical model based upon response surface methodology (RSM) is developed to study the variation of surface roughness with changing process parameter settings. Surface roughness of the test slabs produced with different parameter settings and thickness has been studied under confocal microscope. Response surface.
Negative Affect Reduces Performance in Implicit Sequence Learning

Background It is well documented that positive rather than negative moods encourage integrative processing of conscious information. However, the extent to which implicit or unconscious learning can be influenced by affective states remains unclear. Methodology/Principal Findings A Serial Reaction Time (SRT) task with sequence structures requiring integration over past trials was adopted to examine the effect of affective states on implicit learning. Music was used to induce and maintain positive and negative affective states. The present study showed that participants in negative rather than positive states learned less of the regularity. Moreover, the knowledge was shown by a Bayesian analysis to be largely unconscious as participants were poor at recognizing the regularity. Conclusions/Significance The results demonstrated that negative rather than positive affect inhibited implicit learning of complex structures. Our findings help to understand the effects of affective states on unconscious or implicit processing.
Increased Expression of GAP43 in Interneurons in a Rat Model of Experimental Polymicrogyria

To investigate seizure susceptibility in polymicrogyria, the seizure threshold and growth-associated protein GAP43 expression were analyzed in a rat experimental model of polymicrogyria induced by intracerebral injection of ibotenate. A total of 72 neonates from 9 pregnant rats were used. Intraperitoneal pentylenetetrazole injection did not induce any seizure activity in the control rats, although it elicited seizures of variable severity in the polymicrogyria rats. Fluoro-Jade B–positive degenerating interneurons were found in the polymicrogyria brains; however, no such neurons were detected in the control brains. In the polymicrogyria rats, the GAP43 expression was significantly and widely distributed in the brain, and the percentage of parvalbumin-positive interneurons in the GAP43-positive cells was significantly higher than that observed in the nonphosphorylated neurofilament-positive pyramidal cells. We conclude that the relatively selective vulnerability of inhibitory interneurons constitutes the basis for the decreased seizure threshold observed in this model of polymicrogyria.
The Hardest X-Ray Source in the ASCA Large Sky Survey: Discovery of a New Type 2 Seyfert

We present results of the ASCA deep-exposure observations of the hardest X-ray source discovered in the ASCA Large Sky Survey (LSS) project, designated as AX J131501+3141. We extract its accurate X-ray spectrum, taking into account the contamination from a nearby soft source (AX J131502+3142) separated from it by only 1'. AX J131501+3141 exhibits a large absorption of N H =6+ 4−2×1022 H cm-2, with a photon index Γ=1.5−0.6+0.7. The 2-10 keV flux was about 5 × 10-13 ergs cm-2 s-1, and was time variable by a factor of 30% in 0.5 yr. From the highly absorbed X-ray spectrum and the time variability, as well as the results of the optical follow-up observations, we conclude that AX J131501+3141 is a type 2 Seyfert galaxy. Discovery of such a low-flux and highly absorbed X-ray source could have a significant impact on our understanding of the origin of the cosmic X-ray background.
Acetylation modulates prolactin receptor dimerization

Cytokine-activated receptors undergo extracellular domain dimerization, which is necessary to activate intracellular signaling pathways. Here, we report that in prolactin (PRL)-treated cells, PRL receptor (PRLR) undergoes cytoplasmic loop dimerization that is acetylation-dependent. PRLR-recruited CREB-binding protein (CBP) acetylates multiple lysine sites randomly distributed along the cytoplasmic loop of PRLR. Two PRLR monomers appear to interact with each other at multiple parts from the membrane-proximal region to the membrane-distal region, relying on the coordination among multiple lysine sites neutralized via acetylation. Cytoplasmic loop-dimerized PRLR activates STAT5, which is also acetylated by CBP and undergoes acetylation-dependent dimerization. PRLR dimerization and subsequent signaling are enhanced by treating the cells with deacetylase sirtuin (SIRT) inhibitor nicotinamide or histone deacetylase (HDAC) inhibitor trichostatin A but inhibited by expressing exogenous deacetylase SIRT2 or HDAC6. Our results suggest that acetylation and deacetylation provide the rheostat-like regulation for the cytokine receptor PRLR in its cytoplasmic loop dimerization and subsequent STAT5 activation.
A scene-based imitation framework for RoboCup clients

We describe an effort to train a RoboCup soccer-playing agent playing in the Simulation League by capturing data from existing players, and using it in a real-time scene recognition system. When observing a simple rule-based, stateless agent, the trained player appears to imitate the behaviour of the original. Apart from some parameter selections, the process requires little human intervention.
Tunnel Enlargement and Coalition After Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction With Hamstring Tendon Autografts

Background: Tunnel enlargement and coalition following double-bundle anterior cruciate ligament (ACL) reconstruction with hamstring tendon autografts has not yet been sufficiently studied. Hypothesis: The incidence and the degree of femoral tunnel enlargement will be significantly greater than those for tibial tunnel enlargement after anatomic double-bundle ACL reconstruction using hamstring tendon autografts. There will be no significant correlation between tunnel enlargement and coalition and the postoperative knee laxity. Study Design: Case series; Level of evidence, 4. Methods: Thirty-nine patients who underwent anatomic double-bundle ACL reconstruction using semitendinosus and gracilis tendon autografts were followed up for 1 year after surgery. The grafts were simultaneously fixed at 10° of knee flexion with EndoButtons and spiked staples. All patients were examined with computed tomography and the standard clinical evaluation methods at 2 weeks and 1 year after surgery. Results: The degree of tunnel enlargement of the femoral anteromedial and posterolateral tunnels averaged 10% to 11% and 7% to 9%, respectively, while that of the tibial anteromedial and posterolateral tunnels averaged 3% to 7% and 1% to 6%. The degree and incidence of the anteromedial and posterolateral tunnel enlargement were significantly greater in the femur than in the tibia (P < .0335 and P < .0405, respectively). On the femoral and tibial intra-articular surface, tunnel outlet coalition was found in 5% and 77% of the knees, respectively, at 1 year after surgery. There was no significant correlation between tunnel enlargement and coalition and the clinical outcome. Conclusion: The incidence and the degree of each tunnel enlargement in the femur were significantly greater than that in the tibia. However, the incidence of tunnel coalition in the femur was significantly less than that in the tibia after double-bundle ACL reconstruction with a transtibial technique. There was no significant correlation between tunnel enlargement and coalition and the clinical outcome. Clinical Relevance: The present study provides orthopaedic surgeons with important information on double-bundle ACL reconstruction with hamstring tendons.
A scenario-based approach for direct interruptability prediction on wearable devices

Purpose – People are subjected to a multitude of interruptions. In order to manage these interruptions it is imperative to predict a person's interruptability – his/her current readiness or inclination to be interrupted. This paper aims to introduce the approach of direct interruptability inference from sensor streams (accelerometer and audio data) in a ubiquitous computing setup and to show that it provides highly accurate and robust predictions.Design/methodology/approach – The authors argue that scenarios are central for evaluating the performance of ubiquitous computing devices (and interruptability predicting devices in particular) and prove this on the setup employed, which was based on that of Kern and Schiele.Findings – The paper demonstrates that scenarios provide the foundation for avoiding misleading results, and provide the basis for a stratified scenario‐based learning model, which greatly speeds up the training of such devices.Practical implications – The direct prediction seems to be compet.
Component Based Design of Multitolerant Systems

The concept of multitolerance abstracts problems in system dependability and provides a basis for improved design of dependable systems. In the abstraction, each source of undependability in the system is represented as a class of faults, and the corresponding ability of the system to deal with that undependability source is represented as a type of tolerance. Multitolerance thus refers to the ability of the system to tolerate multiple fault classes, each in a possibly different way. We present a component based method for designing multitolerance. Two types of components are employed by the method, namely detectors and correctors. A theory of detectors, correctors, and their interference free composition with intolerant programs is developed, which enables stepwise addition of components to provide tolerance to a new fault class while preserving the tolerances to the previously added fault classes. We illustrate the method by designing a fully distributed multitolerant program for a token ring.
Predawn and high intensity application of supplemental blue light decreases the quantum yield of PSII and enhances the amount of phenolic acids, flavonoids, and pigments in Lactuca sativa

To evaluate the effect of blue light intensity and timing, two cultivars of lettuce [Lactuca sativa cv. “Batavia” (green) and cv. “Lollo Rossa” (red)] were grown in a greenhouse compartment in late winter under natural light and supplemental high pressure sodium (SON-T) lamps yielding 90 (±10) μmol m−2 s−1 for up to 20 h, but never between 17:00 and 21:00. The temperature in the greenhouse compartments was 22/11°C day/night, respectively. The five light-emitting diode (LED) light treatments were Control (no blue addition), 1B 06-08 (Blue light at 45 μmol m−2 s−1 from 06:00 to 08:00), 1B 21-08 (Blue light at 45 μmol m−2 s−1 from 21:00 to 08:00), 2B 17-19 (Blue at 80 μmol m−2 s−1 from 17:00 to 19:00), and 1B 17-19 (Blue at 45 μmol m−2 s−1 from 17:00 to 19:00). Total fresh and dry weight was not affected with additional blue light; however, plants treated with additional blue light were more compact. The stomatal conductance in the green lettuce cultivar was higher for all treatments with blue light compared to the Control. Photosynthetic yields measured with chlorophyll fluorescence showed different response between the cultivars; in red lettuce, the quantum yield of PSII decreased and the yield of non-photochemical quenching increased with increasing blue light, whereas in green lettuce no difference was observed. Quantification of secondary metabolites showed that all four treatments with additional blue light had higher amount of pigments, phenolic acids, and flavonoids compared to the Control. The effect was more prominent in red lettuce, highlighting that the results vary among treatments and compounds. Our results indicate that not only high light level triggers photoprotective heat dissipation in the plant, but also the specific spectral composition of the light itself at low intensities. However, these plant responses to light are cultivar dependent.
Hoxc Gene Collinear Expression and Epigenetic Modifications Established during Embryogenesis Are Maintained until after Birth

The Hox genes, which are organized into clusters on different chromosomes, are key regulators of embryonic anterior-posterior (A-P) body pattern formation and are expressed at specific times and in specific positions in developing vertebrate embryos. Previously, we have shown that histone methylation patterns are closely correlated with collinear Hox gene expression patterns along the A-P axis of E14.5 mouse embryos. Since histone modification is thought to play a crucial mechanistic role in the highly coordinated pattern of collinear Hox gene expression, we examined the maintenance of the spatial collinear expression pattern of Hoxc genes and the corresponding histone modifications during embryogenesis and in early postnatal mice. Hox expression patterns and histone modifications were analyzed by semi-quantitative RT-PCR and chromatin immunoprecipitation (ChIP)-PCR analyses, respectively. The spatiotemporal expression patterns of Hoxc genes in a cluster were maintained until the early postnatal stage (from E8.5 through P5). Examination of histone modifications in E14.5 and P5 tissues revealed that level of H3K27me3 is only a weak correlation with collinear Hoxc gene expression in the trunk regions although diminished in general, however the enrichment of H3K4me3 is strongly correlated with the gene expression in both stages. In summary, the initial spatiotemporal collinear expression pattern of Hoxc genes and epigenetic modifications are maintained after birth, likely contributing to the establishment of the gene expression code for position in the anatomic body axis throughout the entire life of the organism.
Coffee, tea, caffeine and risk of depression: A systematic review and dose-response meta-analysis of observational studies.

SCOPE The aim of the study was to systematically review and analyze results from observational studies on coffee, caffeine, and tea consumption and association or risk of depression. METHODS AND RESULTS Embase and PubMed databases were searched from inception to June 2015 for observational studies reporting the odds ratios or relative risks (RRs) and 95% confidence intervals (CI) of depression by coffee/tea/caffeine consumption. Random effects models, subgroup analyses, and dose-response analyses were performed. Twelve studies with 23 datasets were included in the meta-analysis, accounting for a total of 346 913 individuals and 8146 cases of depression. Compared to individuals with lower coffee consumption, those with higher intakes had pooled RR of depression of 0.76 (95% CI: 0.64, 0.91). Dose-response effect suggests a nonlinear J-shaped relation between coffee consumption and risk of depression with a peak of protective effect for 400 mL/day. A borderline nonsignificant association between tea consumption and risk of depression was found (RR 0.70, 95% CI: 0.48, 1.01), while significant results were found only for analysis of prospective studies regarding caffeine consumption (RR = 0.84, 95% CI: 0.75, 0.93). CONCLUSION This study suggests a protective effect of coffee and, partially, of tea and caffeine on risk of depression.