Miracle-dz/UniFAR-FTUs huggingface dataset:
Ultrafast energy relaxation in single light-harvesting complexes

Significance The excitation energy transfer in light-harvesting complexes is usually studied either by ultrafast bulk spectroscopy or by single-molecule spectroscopy. These methods are to a high degree complementary: Bulk spectroscopy measures ultrafast processes averaged over thousands of complexes, whereas single-molecule spectroscopy observes much slower dynamics in individual complexes. In this work, we combine these approaches using a recently developed ultrafast single-molecule spectroscopy technique. This enables us, for the first time (to our knowledge), to observe ultrafast energy relaxation with a rate around 100 fs in individual light-harvesting complexes. We determine the distribution of the relaxation times, observe changes of the relaxation time in one complex, and find how the relaxation depends on excitation wavelength. Energy relaxation in light-harvesting complexes has been extensively studied by various ultrafast spectroscopic techniques, the fastest processes being in the sub–100-fs range. At the same time, much slower dynamics have been observed in individual complexes by single-molecule fluorescence spectroscopy (SMS). In this work, we use a pump–probe-type SMS technique to observe the ultrafast energy relaxation in single light-harvesting complexes LH2 of purple bacteria. After excitation at 800 nm, the measured relaxation time distribution of multiple complexes has a peak at 95 fs and is asymmetric, with a tail at slower relaxation times. When tuning the excitation wavelength, the distribution changes in both its shape and position. The observed behavior agrees with what is to be expected from the LH2 excited states structure. As we show by a Redfield theory calculation of the relaxation times, the distribution shape corresponds to the expected effect of Gaussian disorder of the pigment transition energies. By repeatedly measuring few individual complexes for minutes, we find that complexes sample the relaxation time distribution on a timescale of seconds. Furthermore, by comparing the distribution from a single long-lived complex with the whole ensemble, we demonstrate that, regarding the relaxation times, the ensemble can be considered ergodic. Our findings thus agree with the commonly used notion of an ensemble of identical LH2 complexes experiencing slow random fluctuations.
Targeting cytosolic phospholipase A2 α in colorectal cancer cells inhibits constitutively activated protein kinase B (AKT) and cell proliferation

A constitutive activation of protein kinase B (AKT) in a hyper-phosphorylated status at Ser473 is one of the hallmarks of anti-EGFR therapy-resistant colorectal cancer (CRC). The aim of this study was to examine the role of cytosolic phospholipase A2α (cPLA2α) on AKT phosphorylation at Ser473 and cell proliferation in CRC cells with mutation in phosphoinositide 3-kinase (PI3K). AKT phosphorylation at Ser473 was resistant to EGF stimulation in CRC cell lines of DLD-1 (PIK3CAE545K mutation) and HT-29 (PIK3CAP499T mutation). Over-expression of cPLA2α by stable transfection increased basal and EGF-stimulated AKT phosphorylation and proliferation in DLD-1 cells. In contrast, silencing of cPLA2α with siRNA or inhibition with Efipladib decreased basal and EGF-stimulated AKT phosphorylation and proliferation in HT-29. Treating animals transplanted with DLD-1 with Efipladib (10 mg/kg, i.p. daily) over 14 days reduced xenograft growth by >90% with a concomitant decrease in AKT phosphorylation. In human CRC tissue, cPLA2α expression and phosphorylation were increased in 63% (77/120) compared with adjacent normal mucosa determined by immunohistochemistry. We conclude that cPLA2α is required for sustaining AKT phosphorylation at Ser473 and cell proliferation in CRC cells with PI3K mutation, and may serve as a potential therapeutic target for treatment of CRC resistant to anti-EGFR therapy.
An Evolutionary Game for Distributed Resource Allocation in Self-Organizing Small Cells

We propose an evolutionary game theory (EGT)-based distributed resource allocation scheme for small cells underlaying a macro cellular network. EGT is a suitable tool to address the problem of resource allocation in self-organizing small cells since it allows the players with bounded-rationality to learn from the environment and take individual decisions for attaining the equilibrium with minimum information exchange. EGT-based resource allocation can also provide fairness among users. We show how EGT can be used for distributed subcarrier and power allocation in orthogonal frequency-division multiple access (OFDMA)-based small cell networks while limiting interference to the macrocell users below given thresholds. Two game models are considered, where the utility of each small cell depends on average achievable signal-to-interference-plus-noise ratio (SINR) and data rate, respectively. Forthe proposed distributed resource allocation method, the average SINR and data rate are obtained based on a stochastic geometry analysis. Replicator dynamics is used to model the strategy adaptation process of the small cell base stations and an evolutionary equilibrium is obtained as the solution. Based on the results obtained using stochastic geometry, the stability of the equilibrium is analyzed. We also extend the formulation by considering information exchange delay and investigate its impact on the convergence of the algorithm. Numerical results are presented to validate ourtheoretical findings and to show the effectiveness of the proposed scheme in comparison to a centralized resource allocation scheme.
Men's wilderness experience and spirituality: a qualitative study

Previous research on wilderness experience and spirituality focuses on participants in women-only or mixed male and female groups. This qualitative research study investigated the spiritual impact of participation in a men-only canoe journey into a remote wilderness area. In-depth interviews were conducted with six participants after the trip. Interpretive analysis was used to identify themes in the interview data. The results indicated that the short-term impact of participation in the wilderness trip was a sense of peacefulness while the long-term impact was characterized by recollection. The wilderness setting, along with the diverse social settings during the trip, was viewed as important to spiritual well-being. Most of the participants did not identify the scheduled spiritual activities as having a large impact upon their spiritual well-being. A few, but not all, of the participants viewed participation in a men-only group as important to their spiritual well-being.
A multilevel framework for recruiting and supporting graduate students from culturally diverse backgrounds in school psychology programs

The lack of cultural diversity among practitioners and trainers in the field of school psychology has been recognized as a longstanding problem. In particular, individuals from racial, ethnic, and linguistic minority and international backgrounds often encounter a range of barriers to pursuing graduate study in school psychology. Given the urgent need to increase diversity among school psychologists, faculty and institutions must take proactive measures to deconstruct these barriers and to support the success of all students. This article outlines a multilevel framework for recruiting and supporting graduate students from culturally diverse backgrounds in school psychology programs. Within this framework, research-based strategies are presented at the primary, secondary, and tertiary levels of support. Moreover, considerations for assessing program and student outcomes are discussed, and applications to school psychology programs internationally are considered.
Inactivation of Intergenic Enhancers by EBNA3A Initiates and Maintains Polycomb Signatures across a Chromatin Domain Encoding CXCL10 and CXCL9

Epstein-Barr virus (EBV) causes a persistent infection in human B cells by establishing specific transcription programs to control B cell activation and differentiation. Transcriptional reprogramming of EBV infected B cells is predominantly driven by the action of EBV nuclear antigens, among them the transcriptional repressor EBNA3A. By comparing gene expression profiles of wt and EBNA3A negative EBV infected B cells, we have previously identified a broad array of cellular genes controlled by EBNA3A. We now find that genes repressed by EBNA3A in these cells are significantly enriched for the repressive histone mark H3K27me3, which is installed by Polycomb group (PcG) proteins. This PcG-controlled subset of genes also carries H3K27me3 marks in a variety of other tissues, suggesting that the commitment to PcG silencing is an intrinsic feature of these gene loci that can be used by EBNA3A. In addition, EBNA3A targets frequently reside in co-regulated gene clusters. To study the mechanism of gene repression by EBNA3A and to evaluate the relative contribution of PcG proteins during this process, we have selected the genomic neighbors CXCL10 and CXCL9 as a model for co-repressed and PcG-controlled genes. We show that EBNA3A binds to CBF1 occupied intergenic enhancers located between CXCL10 and CXCL9 and displaces the transactivator EBNA2. This impairs enhancer activity, resulting in a rapid transcriptional shut-down of both genes in a CBF1-dependent manner and initiation of a delayed gain of H3K27me3 marks covering an extended chromatin domain. H3K27me3 marks increase gradually and are maintained by EBNA3A. Our study provides direct evidence that repression by EBNA3A requires CBF1 and that EBNA3A and EBNA2 compete for access to CBF1 at identical genomic sites. Most importantly, our results demonstrate that transcriptional silencing by EBNA3A precedes the appearance of repressive PcG marks and indicate that both events are triggered by loss of enhancer activity.
Acute Kidney Injury Following Cardiopulmonary Bypass in Children　- Risk Factors and Outcomes.

BACKGROUND Acute kidney injury (AKI) is the most common and most serious complication following heart surgery. We aimed to determine the prevalence of, and risk factors for, AKI following pediatric cardiac surgery.Methods and Results:We retrospectively analyzed 135 patients aged ≤18 years who underwent cardiac surgery for congenital heart defects; by RACHS-1 category, 58 patients (43%) had an operative risk score ≥3. AKI was defined and classified using the pediatric pRIFLE criteria (Pediatric Risk, Injury, Failure, Loss, and End-stage Kidney Disease); 19 patients (14.1%) developed AKI: 17 had AKI with a severity classified as risk (R) and 2 had AKI classified as injury (I). Body weight, height, body surface area, and preoperative mechanical ventilation were all independently associated with AKI development (P=0.038, 0.040, 0.033 and 0.008, respectively). Preoperative ventilation strongly correlated with AKI severity. Higher pRIFLE classification positively correlated with increased incidence of peritoneal dialysis, increased postoperative mechanical ventilation duration, and longer hospital stay (P=0.009, 0.039 and 0.042, respectively). CONCLUSIONS In this study, we found a low prevalence of postoperative AKI in pediatric patients undergoing severe cardiac surgery. AKI was associated with worse early postoperative outcomes. Early prediction and appropriate treatment of AKI during the postoperative period are emphasized.
Bodies and language: process drama and intercultural language learning in a beginner language classroom

In this article the author draws on classroom video recordings and student commentary to explore ways in which the kinaesthetic elements of a process drama provided the context and the space for beginner additional language learners to engage with intercultural language learning. In the light of student comments in interviews and questionnaires, she justifies the need for learners to experience the bodily aspect of communication as part of authentic, multi-modal interaction and hence a crucial element in intercultural literacy. Drawing on Kress's concept of interest as a motivation to make meaning, she suggests that learner interests are aroused by dramatic conventions whose multi-modal nature affords students access to multiple ways of participating in the narrative. She argues that a more conscious integration of the kinaesthetic mode into the additional language classroom can stimulate, scaffold and authenticate the verbal participation of beginner learners. Finally, given the students’ overwhelmingly positive response to the kinaesthetic elements of the work, she suggests that, despite the challenge of balancing drama and language imperatives, encouraging language teachers to venture into imaginative worlds and include a kinaesthetic dimension to their communicative practice could improve engagement and outcomes for beginner learners.
France in Indochina: Colonial Encounters

Valorized as 'la perle de l'Extreme Orient', Indochina was France's rival to Britain's 'jewel in the crown'. Advanced, worthy, and accorded special status, it was a showcase of success, but also a site of disaster. Given the current scholarly interest in reassessing colonial attitudes and in francophone culture, this book fills an important gap by focusing upon the neglected French colonial discourses at the height of the French imperial encounter with Indochina. The period of French colonial rule in Indochina spanned some ninety years and not only did it witness France's Fourth Republic's first experience (and loss) of colonial war, it also exemplified the often contradictory representations and perceptions of imperial identity, colonialism and the legacy of the 1789 Revolution. Framed by political, ideological and historical developments and debates, each chapter develops an intriguing socio-cultural account of France's own understanding of its role in Indochina and its relationship with the colony. The author brings together striking images from colonial expositions, metropolitan fiction, travel journalism, world exhibitions, popular song, gendered and familial representations as well as film to reveal the confusion over imperial identity that prevailed in France until the eve of the Second World War. This authoritative work provides an important re-evaluation of French Indochina and its legacy. Its interdisciplinary approach will be of interest to a broad readership: students of French history, colonial and postcolonial studies, cultural studies, literature, sociology and race.
Axonogenesis in the medaka embryonic brain

In order to know the general pattern of axonogenesis in vertebrates, we examined axonogenesis in the embryonic brain of a teleost fish, medaka (Oryzias latipes), and the results were compared with previous studies in zebrafish and mouse. The axons and somata were stained immunocytochemically using antibodies to a cell surface marker (HNK‐1) and acetylated tubulin and visualized by retrograde and anterograde labeling with a lipophilic dye. The fiber systems developed correlating with the organization of the longitudinal and transverse subdivisions of the embryonic brain. The first axons extended from the synencephalic tegmentum, forming the first fiber tract (fasciculus longitudinalis medialis) in the ventral longitudinal zone of the neural rod, 38 hours after fertilization. In the neural tube, throughout the entire brain two pairs of longitudinal fiber systems, one ventral series and one dorsal or intermediate series, and four pairs of transverse fiber tracts in the rostral brain were formed sequentially during the first 16 hours of axon production. In one of the dorsal longitudinal tracts, its branch retracted and disappeared at later stages. One of the transverse tracts was found to course in the telencephalon and hypothalamus. The overall pattern of the longitudinal fiber systems in medaka brain is similar to that in mouse, but apparently different from that in zebrafish. We propose that a ventral tract reported in zebrafish partially belongs to the dorsal fiber system, and that the longitudinal fiber systems in all vertebrate brains pass through a common layout defined by conserved genetic and developmental programs. J. Comp. Neurol. 476:240–253, 2004. © 2004 Wiley‐Liss, Inc.
Enhanced Biologically Inspired Model for Object Recognition

The biologically inspired model (BIM) proposed by Serre presents a promising solution to object categorization. It emulates the process of object recognition in primates' visual cortex by constructing a set of scale- and position-tolerant features whose properties are similar to those of the cells along the ventral stream of visual cortex. However, BIM has potential to be further improved in two aspects: mismatch by dense input and randomly feature selection due to the feedforward framework. To solve or alleviate these limitations, we develop an enhanced BIM (EBIM) in terms of the following two aspects: 1) removing uninformative inputs by imposing sparsity constraints, 2) apply a feedback loop to middle level feature selection. Each aspect is motivated by relevant psychophysical research findings. To show the effectiveness of the EBIM, we apply it to object categorization and conduct empirical studies on four computer vision data sets. Experimental results demonstrate that the EBIM outperforms the BIM and is comparable to state-of-the-art approaches in terms of accuracy. Moreover, the new system is about 20 times faster than the BIM.
Evaluation of Leakage Reduction Alternatives for Deep Submicron Dynamic Nonuniform Cache Architecture Caches

Wire delays and leakage energy consumption are both growing problems in designing large on-chip caches. Nonuniform cache architecture (NUCA) is a wire-delay aware design paradigm based on the sub-banking of a cache, which allows the banks closer to the controller to be accessed with reduced latencies with respect to the other banks. This feature is leveraged by dynamic NUCA (D-NUCA) caches via a migration mechanism which speeds up frequently used data access, further reducing the effect wire delays have on performance. To reduce leakage power consumption of static random access memory caches, various micro-architectural techniques have been proposed. In this brief, we compare the benefits and limits of the application of some of these techniques to a D-NUCA cache memory, and propose a novel hybrid scheme based on the Drowsy and Way Adaptable techniques. Such a scheme allows further improvement in leakage reduction and limits the impact of process variation on the effectiveness of the Drowsy technique.
Availability of nutritional support services in HIV care and treatment sites in sub-Saharan African countries

Abstract Objective To examine the availability of nutritional support services in HIV care and treatment sites across sub-Saharan Africa. Design In 2008, we conducted a cross-sectional survey of sites providing antiretroviral therapy (ART) in nine sub-Saharan African countries. Outcomes included availability of: (i) nutritional counselling; (ii) micronutrient supplementation; (iii) treatment for severe malnutrition; and (iv) food rations. Associations with health system indicators were explored using bivariate and multivariate methods. Setting President's Emergency Plan for AIDS Relief-supported HIV treatment and care sites across nine sub-Saharan African countries. Subjects A total of 336 HIV care and treatment sites, serving 467 175 enrolled patients. Results Of the sites under study, 303 (90 %) offered some form of nutritional support service. Nutritional counselling, micronutrient supplementation, treatment for severe acute malnutrition and food rations were available at 98 %, 64 %, 36 % and 31 % of sites, respectively. In multivariate analysis, secondary or tertiary care sites were more likely to offer nutritional counselling (adjusted OR (AOR): 2·2, 95 % CI 1·1, 4·5). Rural sites (AOR: 2·3, 95 % CI 1·4, 3·8) had increased odds of micronutrient supplementation availability. Sites providing ART for >2 years had higher odds of availability of treatment for severe malnutrition (AOR: 2·4, 95 % CI 1·4, 4·1). Sites providing ART for >2 years (AOR: 1·6, 95 % CI 1·3, 1·9) and rural sites (AOR: 2·4, 95 % CI 1·4, 4·4) had greater odds of food ration availability. Conclusions Availability of nutritional support services was high in this large sample of HIV care and treatment sites in sub-Saharan Africa. Further efforts are needed to determine the uptake, quality and effectiveness of these services and their impact on patient and programme outcomes.
Time-Dependent Relationship between the Dorsal Hippocampus and the Prefrontal Cortex in Spatial Memory

The prefrontal cortex and the dorsal hippocampus have been studied extensively for their significant roles in spatial working memory. A possible time-dependent functional relationship between the prefrontal cortex and the dorsal hippocampus in spatial working memory was tested. A combined lesion and pharmacological inactivation technique targeting both the dorsal hippocampus and the medial prefrontal cortex was used (i.e., axon-sparing lesions of the dorsal hippocampus combined with reversible inactivation of the medial prefrontal cortex, or vice versa, within a subject). A delayed nonmatching-to-place task on a radial eight-arm maze with short-term (i.e., 10 sec) versus intermediate-term (i.e., 5 min) delays was used as a behavioral paradigm. Here we report that the dorsal hippocampus and the medial prefrontal cortex process short-term spatial memory in parallel, serving as a compensatory mechanism for each other. The role of the dorsal hippocampus, however, becomes highlighted as the time-window for memory (i.e., delay) shifts from short-term to a delay period (i.e., intermediate-term) exceeding the short-term range. The results indicate that the time window of memory is a key factor in dissociating multiple memory systems.
A New Technique for the Estimation of Jitter and Shimmer of Voiced Speech Signal

This paper presents a new technique for the estimation of jitter and shimmer exploiting the detected value of the pitch-period and amplitude of voiced speech signal degraded by white noise. Perturbations of the glottis cycle duration (jitter) and intonation (shimmer) are, respectively, the irregularities of pitch-period and energy. We propose a modified circular autocorrelation function (MCACF) of the normalized hamming-windowed pre-filtered speech. A weighted and pitch-harmonic MCACF is utilized to effectively extract pitch-period for jitter computation. To carry out shimmer analysis, peak-to-peak amplitude of signal around the estimated pitch-period is optimally obtained by matching the windowed pre-filtered speech frame with a shifted impulse train. For performance evaluation vowels, such as "a, e, i, o, u", are used from the TIMIT database. The conventional algorithms manifest not only the distortion of pitch contours but also provides elevated values of the jitter and shimmer that can be interpreted as the presence of anomalies of the vocal tract, the glottis or the neuro-mechanism. On the contrary, for both clean and noisy speech, the proposed method results in a comparatively smooth pitch contour as well as small values of the jitter and shimmer confirming the presence of a normal voice with good stability and periodicity