Miracle-dz/UniFAR-FTUs huggingface dataset:
Proteome Analysis Reveals Extensive Light Stress-Response Reprogramming in the Seagrass Zostera muelleri (Alismatales, Zosteraceae) Metabolism

Seagrasses are marine ecosystem engineers that are currently declining in abundance at an alarming rate due to both natural and anthropogenic disturbances in ecological niches. Despite reports on the morphological and physiological adaptations of seagrasses to extreme environments, little is known of the molecular mechanisms underlying photo-acclimation, and/or tolerance in these marine plants. This study applies the two-dimensional isoelectric focusing (2D-IEF) proteomics approach to identify photo-acclimation/tolerance proteins in the marine seagrass Zostera muelleri. For this, Z. muelleri was exposed for 10 days in laboratory mesocosms to saturating (control, 200 μmol photons m−2 s−1), super-saturating (SSL, 600 μmol photons m−2 s−1), and limited light (LL, 20 μmol photons m−2 s−1) irradiance conditions. Using LC-MS/MS analysis, 93 and 40 protein spots were differentially regulated under SSL and LL conditions, respectively, when compared to the control. In contrast to the LL condition, Z. muelleri robustly tolerated super-saturation light than control conditions, evidenced by their higher relative maximum electron transport rate and minimum saturating irradiance values. Proteomic analyses revealed up-regulation and/or appearances of proteins belonging to the Calvin-Benson and Krebs cycle, glycolysis, the glycine cleavage system of photorespiration, and the antioxidant system. These proteins, together with those from the inter-connected glutamate-proline-GABA pathway, shaped Z. muelleri photosynthesis and growth under SSL conditions. In contrast, the LL condition negatively impacted the metabolic activities of Z. muelleri by down-regulating key metabolic enzymes for photosynthesis and the metabolism of carbohydrates and amino acids, which is consistent with the observation with lower photosynthetic performance under LL condition. This study provides novel insights into the underlying molecular photo-acclimation mechanisms in Z. muelleri, in addition to identifying protein-based biomarkers that could be used as early indicators to detect acute/chronic light stress in seagrasses to monitor seagrass health.
Estimation of human corneal oxygen consumption by noninvasive measurement of tear oxygen tension while wearing hydrogel lenses.

PURPOSE To devise a procedure for direct estimation of corneal oxygen consumption in human subjects. METHODS Tear oxygen tension (PO2) was measured at the posterior surface of two standard hydrogel contact lenses (38% water, 0.2 and 0.06 mm thick, oxygen transmissibility [Dk/t] = 4.2 and 14 x 10(-9) cm x mL O2/mL x sec x torr) and one newly available hydrogel-silicone polymer lens (Dk/t = 99 x 10(-9)). The oxygen-sensitive dye, Pd-meso-tetra (4-carboxyphenyl) porphine, bound to bovine serum albumin, was incubated with the lenses overnight. The lenses, coated with the protein-dye complex, were placed on four subjects' eyes, and tear PO2 was measured in the open eye and after 5 minutes of eye closure, using a time-domain phosphorescence measurement system. Given the tear PO2, lens Dk/t, and corneal thickness, oxygen consumption (Q(C), in mL O2/cm(3) x sec) could be calculated from established oxygen diffusion models. RESULTS Protein-dye complex bound to the lens surface enabled reporting of tear PO2 for long periods. As expected, estimated tear PO2 was higher in subjects wearing lenses with higher Dk/t: mean open-eye PO2 = 30.6 +/- 3.1 and 8.1 +/- 1.3 torr for the thin and thick hydrogel lenses, respectively, and 97.6 +/- 22.9 torr for the hydrogel-silicone lens. After 5 minutes of eye closure, tear PO2 was significantly reduced and reached a new steady state in approximately 20 seconds after eye opening. Fitting a single exponential model to the data and extrapolating to t = 0 provided an estimate of PO2 under the closed lid for the thin hydrogel (PO2 = 7 +/- 2.3 torr) and the hydrogel-silicone lens (PO2 = 22.6 +/- 4 torr). After 5 minutes of eye closure with the thick hydrogel lens, tear PO2 remained constant for approximately 10 seconds after eye opening (mean PO2 = 3.9 +/- 0.7) before increasing to a new steady state. This delay could be accounted for by the time needed for oxygen to diffuse to the posterior surface of the lens. Calculated Q(C) ranged from 2.2 x 10(-4) to 3.7 x 10(-6) mL O2/cm(3) x sec) at the highest and lowest PO2s, respectively, and is comparable to previous in vitro and in vivo estimates. CONCLUSIONS Tear PO2 behind hydrogel lenses can be measured in human subjects using the phosphorescence of the porphyrin-protein complex bound to the lens surface. The method is simple, fast, reliable, and noninvasive, allowing quick and direct estimates of Q(C). In addition to contact lens wear, this method should be useful for examining the effects of disease, surgery, or topical drugs on the corneal oxygen consumption rate.
Alterations of Cardiac Structure in Patients With Isolated Office, Ambulatory, or Home Hypertension: Data From the General Population (Pressione Arteriose Monitorate E Loro Associazioni [PAMELA] Study)

Background—The prevalence and clinical significance of isolated office (or white coat) hypertension is controversial, and population data are limited. We studied the prevalence of this condition and its association with echocardiographic left ventricular mass in the general population of the PAMELA (Pressione Arteriose Monitorate E Loro Associazioni) Study. Methods and Results—The study involved a large, randomized sample (n=3200) representative of the Monza (Milan) population, 25 to 74 years of age. Participants in the study (64% of the sample) underwent measurements of office, home, 24-hour ambulatory blood pressure, and echocardiography. Isolated office hypertension was defined as systolic or diastolic values ≥140 mm Hg or ≥90 mm Hg, respectively. Home and ambulatory normotension were defined according to criteria previously established from the PAMELA Study, for example, <132/83 mm Hg (systolic/diastolic) for home and 125/79 mm Hg for 24-hour average blood pressure. Treated hypertensive subjects were excluded from analysis that was made on a total of 1637 subjects. Depending on normotension being established on systolic or diastolic blood pressure measured at home or over 24 hours, the prevalence of isolated office hypertension ranged from 9% to 12%. In these subjects, left ventricular mass index was greater (P <0.01) than in subjects with normotension both in and outside the office. This was the case also for prevalence of left ventricular hypertrophy. Left ventricular mass index and hypertrophy were similarly greater in subjects found to have normal office but elevated home or ambulatory blood pressure (≈10% of the population). Conclusions—Isolated office hypertension has a noticeable prevalence in the population and is accompanied by structural cardiac alterations, suggesting that it is not an entirely harmless phenomenon. This is the case also for the opposite condition, that is, normal office but elevated home or ambulatory blood pressure, which implies that limiting blood pressure measurements to office values may not suffice in identification of subjects at risk.
When Free Is Not for Me: Confronting the Barriers to Use of Free Quitline Telephone Counseling for Tobacco Dependence

Remarkable disparities in smoking rates in the United States contribute significantly to socioeconomic and minority health disparities. Access to treatment for tobacco use can help address these disparities, but quitlines, our most ubiquitous treatment resource, reach just 1%–2% of smokers. We used community-based participatory methods to develop a survey instrument to assess barriers to use of the quitline in the Arkansas Mississippi delta. Barriers were quitline specific and barriers to cessation more broadly. Over one-third (34.9%) of respondents (n = 799) did not have access to a telephone that they could use for the quitline. Respondents reported low levels of knowledge about the quitline, quitting, and trust in tobacco treatment programs as well as considerable ambivalence about quitting including significant concerns about getting sick if they quit and strong faith-based beliefs about quitting. These findings suggest quitlines are not accessible to all lower socioeconomic groups and that significant barriers to use include barriers to cessation. These findings suggest targets for providing accessible tobacco use treatment services and addressing concerns about cessation among lower income, ethnic minority, and rural groups.
Facts and numbers on epidemiology and pharmacological treatment of heart failure with preserved ejection fraction

Heart failure with preserved ejection fraction (HFpEF) is a major and growing public health problem. Epidemiologic studies demonstrated that heart failure (HF) can be clinically diagnosed in patients with normal or preserved left ventricular ejection fraction. These patients are therefore termed as having HFpEF. In the past, this was often called diastolic HF. Because of the permanent increase of the prevalence of HFpEF during the past decades, HFpEF now accounts for more than 50% of the total HF population. There are uncertainties and debates regarding the definition, diagnosis, and pathophysiology with the consequence that all outcome trials performed so far used criteria for inclusion and exclusion that were not consistent. These trials also failed to document improved prognosis. Recent smaller proof‐of‐concept or Phase II clinical trials investigating different pathophysiological approaches with substances such as the neprilysin inhibitor–angiotensin receptor blocker− combination (LCZ 696), ranolazine, or ivabradine were successful to improve biomarkers, haemodynamics, or functional capacity. Future trials will need to document whether also prognosis can be improved.
Chemoreceptor Evolution in Hymenoptera and Its Implications for the Evolution of Eusociality

Eusocial insects, mostly Hymenoptera, have evolved unique colonial lifestyles that rely on the perception of social context mainly through pheromones, and chemoreceptors are hypothesized to have played important adaptive roles in the evolution of sociality. However, because chemoreceptor repertoires have been characterized in few social insects and their solitary relatives, a comprehensive examination of this hypothesis has not been possible. Here, we annotate ∼3,000 odorant and gustatory receptors in recently sequenced Hymenoptera genomes and systematically compare >4,000 chemoreceptors from 13 hymenopterans, representing one solitary lineage (wasps) and three independently evolved eusocial lineages (ants and two bees). We observe a strong general tendency for chemoreceptors to expand in Hymenoptera, whereas the specifics of gene gains/losses are highly diverse between lineages. We also find more frequent positive selection on chemoreceptors in a facultative eusocial bee and in the common ancestor of ants compared with solitary wasps. Our results suggest that the frequent expansions of chemoreceptors have facilitated the transition to eusociality. Divergent expression patterns of odorant receptors between honeybee and ants further indicate differential roles of chemoreceptors in parallel trajectories of social evolution.
Physical model study of selective slag splashing in the BOF

Steelmaking converter vessels do not usually wear evenly. Often, the higher wear rate in the trunnion area determines the lining life of the vessel. On the other hand, major skull growth may occur in other parts. In order to ensure consistent BOF performance, maintaining the vessel geometry by selective slag coating is important. Since selective slag splashing is difficult to perform by conventional oxygen lance, the effect of e.g. plugging one or two lance nozzles on the amount and direction of splashing was investigated by physical model experiments. Furthermore, the model was used to predict the influence of lance height, lance position, bottom blowing configuration and liquid viscosity on splashing behaviour. Lowering the lance increased the rate of splashing to a certain lance height beyond which it decreased. Similar behaviour was found to apply also to the slag wash coating mechanism. At low lance distance, plugging one lance nozzle increased the amount of splashing on charge pad and trunnion areas and decreased on slag hole side. Generally, bottom blowing increased the amount of slag drops considerably. Positioning of bottom stirring plugs had a clear effect on the direction of splashes.
Estimating Primary Production of Picophytoplankton Using the Carbon-Based Ocean Productivity Model: A Preliminary Study

Picophytoplankton are acknowledged to contribute significantly to primary production (PP) in the ocean while now the method to measure PP of picophytoplankton (PPPico) at large scales is not yet well established. Although the traditional 14C method and new technologies based on the use of stable isotopes (e.g., 13C) can be employed to accurately measure in situ PPPico, the time-consuming and labor-intensive shortage of these methods constrain their application in a survey on large spatiotemporal scales. To overcome this shortage, a modified carbon-based ocean productivity model (CbPM) is proposed for estimating the PPPico whose principle is based on the group-specific abundance, cellular carbon conversion factor (CCF), and temperature-derived growth rate of picophytoplankton. Comparative analysis showed that the estimated PPPico using CbPM method is significantly and positively related (r2 = 0.53, P < 0.001, n = 171) to the measured 14C uptake. This significant relationship suggests that CbPM has the potential to estimate the PPPico over large spatial and temporal scales. Currently this model application may be limited by the use of invariant cellular CCF and the relatively small data sets to validate the model which may introduce some uncertainties and biases. Model performance will be improved by the use of variable conversion factors and the larger data sets representing diverse growth conditions. Finally, we apply the CbPM-based model on the collected data during four cruises in the Bohai Sea in 2005. Model-estimated PPPico ranged from 0.1 to 11.9, 29.9 to 432.8, 5.5 to 214.9, and 2.4 to 65.8 mg C m-2 d-1 during March, June, September, and December, respectively. This study shed light on the estimation of global PPPico using carbon-based production model.
Health-related quality of life in veterans with prevalent total knee arthroplasty and total hip arthroplasty.

OBJECTIVE To study the HRQOL in veterans with prevalent total knee arthroplasty (TKA) or total hip arthroplasty (THA) and compare them with age- and gender-matched US population and control veteran population without these procedures. METHODS A cohort study and cross-sectional survey on veterans obtained demographics and HRQOL with Short-Form 36 for veterans (SF-36V). Veterans were categorized into: primary TKA; primary THA; combination group (>/=1 primary and/or any revision TKA/THA); and control population (no THA/TKA). Multivariable regression compared the physical and mental component summary scores (PCS and MCS scores, respectively) in each group. RESULTS Response rate was 58% (40 508/70 334): 531 with TKA, 254 with THA, 461 constituted the combination and 39 262, the control group. Mean PCS scores in veterans with THA, TKA, and combination group were 2 s.d. lower than the US mean (29.5 +/- 0.8; 30.1 +/- 1.1 and 27.1 +/- 0.8). MCS scores were similar to the US mean (47.3 +/- 0.9; 49.1 +/- 1.2 and 45.6 +/- 0.9). Compared with controls, significantly more veterans in TKA, THA or combination groups had multivariable-adjusted PCS </= 30 (55, 64, 71 and 76%; P < 0.0001); similar proportion had MCS </= 30 (15, 12, 8 and 16%; P = 0.29); and mean scores on SF-36 physical domains (P </= 0.0011), but not mental/emotional domains (P >/= 0.01) were statistically and clinically lower. CONCLUSIONS Profound physical HRQOL deficits exist in veterans with TKA/THA and in combination group compared with age- and gender-matched general US population and with veteran controls. In these groups, these deficits are not attributable to differences in sociodemographics, comorbidity and healthcare access/utilization. Arthroplasty status may be a surrogate for poorer HRQOL and worse outcomes. Future studies are indicated to determine HRQOL deficit causes and interventions to improve HRQOL in patients with arthroplasty.
A Theory of Neurolinguistic Development

This article offers a developmental theory of language and the neural systems that lead to and subserve linguistic capabilities. Early perceptual experience and discontinuities in linguistic development suggest that language develops in four phases that occur in a fixed, interdependent sequence. In each phase of language, a unique ontogenetic function is accomplished. These functions have proprietary neural systems that vary in their degree of specialization. Of particular interest is an analytical mechanism that is responsible for linguistic grammar. This mechanism is time-locked and can only be turned on in the third phase. Confirming evidence is provided by children who are delayed in the second phase of the language learning process. These children store insufficient lexical material to activate their analytic mechanism. Inactivation behaves like damage, shifting language functions to homologous mechanisms in the nondominant hemisphere, thereby increasing functional and anatomical symmetry across the hemispheres. This atypical assembly of neurolinguistic resources produces functional but imperfect command of spoken language and may complicate learning of written language. The theory thus offers a different role for genetics and early experience, and a different interpretation of neuroanatomic findings, from those entertained in most other proposals on developmental language disorders.
Curvature of n-dimensional space curves in grey-value images

Local curvature represents an important shape parameter of space curves which are well described by differential geometry. We have developed an estimator for local curvature of space curves embedded in n-dimensional (n-D) grey-value images. There is neither a segmentation of the curve needed nor a parametric model assumed. Our estimator works on the orientation field of the space curve. This orientation field and a description of local structure is obtained by the gradient structure tensor. The orientation field has discontinuities; walking around a closed contour yields two such discontinuities in orientation. This field is mapped via the Knutsson mapping to a continuous representation; from a n-D vector to a symmetric n2-D tensor field. The curvature of a space curve, a coordinate invariant property, is computed in this tensor field representation. An extensive evaluation shows that our curvature estimation is unbiased even in the presence of noise, independent of the scale of the object and furthermore the relative error stays small.
Pain assessment and management practices in children following surgery of the lower limb.

AIMS To examine paediatric nurses' pain assessment and management practices in relation to postoperative care for children following surgery of a fractured lower limb and to compare these practices with evidence-based guidelines. BACKGROUND Managing pain is one of the most challenging issues in current paediatric practice. The incidence of lower limb fractures is high in children, which often leads to pain and related complications in the postoperative period. DESIGN A retrospective clinical audit study. METHODS A retrospective audit of all medical records (n = 106) was undertaken over two years of children aged 5-15 years who were admitted for surgical procedure for a fractured lower limb. An audit tool was developed to collect data related to children's postoperative pain assessment and management on the day of operation to the third postoperative day. The study was undertaken in a tertiary paediatric hospital in Australia. RESULTS The retrospective audit revealed that assessment and management of children's postoperative pain was inadequate. On average, 75% of children experienced some degree of pain; 50% had moderate to severe pain. Nurses assessed pain less frequently compared to the number of times they were expected to assess pain postoperatively. Most analgesics were prescribed on an 'as needed' basis and patients received significantly lower amounts of analgesics than prescribed amounts. CONCLUSION The clinical audit revealed that addressing children's postoperative analgesic needs was not consistent with evidence-based guidelines. RELEVANCE TO CLINICAL PRACTICE While this study was undertaken in only one hospital, the results are likely to be applicable to other children's hospital settings. Nurses need to be proactive in promoting effective assessment and management of pain in children. The results of this study provide a useful guide for planning and implementing future strategies to improve postoperative pain management in children.
How and why do student teachers use ICT?

This paper examines how and why student teachers made use of information and communication technology (ICT) during a 1-year initial teacher education programme from 2008 to 2009. This is a mixed methods study involving a survey (N = 340) of the entire cohort and a series of semi-structured interviews with a sample of student teachers within the cohort (N = 21). The study explored several themes, including the nature of student teachers' use of ICT; variation in the use of ICT; support for, and constraints on, using ICT; attitudes to ICT and to teaching and learning more generally. It was found that nearly all teachers were receptive to using ICT – more so than their in-service counterparts – and made frequent use of it during their placement (internship) experience. The Interactive Whiteboard (IWB) was central to nearly all student teachers' use of ICT, in good part, because it was already used by their mentors and was widely accessible. Student teachers' use of ICT was categorized in three levels. Routine users focused mostly on the use of the IWB for whole class teaching; extended users gave greater opportunities for pupils to use ICT for themselves; innovative student teachers used ICT in a greater range of contexts and made more effort to overcome barriers such as access. ICT use was seen as emerging from a mix of factors: chiefly student teachers' access to ICT; their feeling of ‘self-efficacy’ when using ICT; and their belief that ICT had a positive impact on learning – in particular, the impact on pupils' behavioural and affective engagement. Factors which influenced ICT use included mentoring, training and support. Limitations on student teachers' use of ICT are explored and it is suggested that new teachers need to be supported in developing a more discerning use as they begin their teaching careers.