Miracle-dz/UniFAR-FTUs huggingface dataset:
A Phosphate Transporter from Medicago truncatula Involved in the Acquisition of Phosphate Released by Arbuscular Mycorrhizal Fungi Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.004861.

Many plants have the capacity to obtain phosphate via a symbiotic association with arbuscular mycorrhizal (AM) fungi. In AM associations, the fungi release phosphate from differentiated hyphae called arbuscules, that develop within the cortical cells, and the plant transports the phosphate across a symbiotic membrane, called the periarbuscular membrane, into the cortical cell. In Medicago truncatula, a model legume used widely for studies of root symbioses, it is apparent that the phosphate transporters known to operate at the root–soil interface do not participate in symbiotic phosphate transport. EST database searches with short sequence motifs shared by known phosphate transporters enabled the identification of a novel phosphate transporter from M. truncatula, MtPT4. MtPT4 is significantly different from the plant root phosphate transporters cloned to date. Complementation of yeast phosphate transport mutants indicated that MtPT4 functions as a phosphate transporter, and estimates of the Km suggest a relatively low affinity for phosphate. MtPT4 is expressed only in mycorrhizal roots, and the MtPT4 promoter directs expression exclusively in cells containing arbuscules. MtPT4 is located in the membrane fraction of mycorrhizal roots, and immunolocalization revealed that MtPT4 colocalizes with the arbuscules, consistent with a location on the periarbuscular membrane. The transport properties and spatial expression patterns of MtPT4 are consistent with a role in the acquisition of phosphate released by the fungus in the AM symbiosis.
Outcome of antenatally suspected congenital cystic adenomatoid malformation of the lung: 10 years’ experience 1991–2001

Objective: To determine the outcome of antenatally suspected congenital cystic adenomatoid malformation of the lung (CCAM) over a 10 year period. Methods: This is a retrospective study of all babies diagnosed antenatally in the Prenatal Diagnosis Unit and delivered in Oxford between 1991 and 2001. Data were obtained from the Oxford Congenital Anomaly Register, theatre records, and histopathology reports. Results: Twenty eight cases of CCAM were diagnosed antenatally. Five pregnancies were terminated. Data are available on all 23 of the pregnancies that continued and resulted in two neonatal deaths and 21 surviving babies. Eleven of the 23 cases (48%) showed some regression of the lesion antenatally, and four of these cases appeared to resolve completely on prenatal ultrasound. Three of the 23 babies (13%) were symptomatic in the early neonatal period, and three developed symptoms shortly afterwards. Seventeen of the 23 babies (74%) were asymptomatic, of whom 12 had abnormalities on chest radiograph or computed tomography scan and had elective surgery. Two babies (8%) had completely normal postnatal imaging, and three had abnormalities which resolved in the first year of life. Seventeen of the 23 babies (74%) had surgery. Histology at surgery was heterogeneous. Of the 23 live births, all 21 survivors (91%) are well at follow up or have been discharged. Conclusions: All babies diagnosed antenatally with CCAM require postnatal imaging with computed tomography irrespective of signs of antenatal resolution. In asymptomatic infants, the recommendations are close follow up and elective surgery for persistent lesions within the first year of life. Histology at surgery was heterogeneous, and this should be considered when counselling parents.
Overview of groundnuts research in Uganda: Past, present and future

The Groundnut Department at National Semi-Arid Research Resources Institute (NaSARRI) is mandated to conduct research on groundnuts ( Arachis hypogaea L) in Uganda. It undertakes research aimed at cultivar development, maintenance and conservation through germplasm collection, characterization, evaluation, breeding, maintenance and generation of appropriate crop management technologies for sustained production. Most of the varieties traditionally grown by farmers in Uganda are landraces adapted more for survival than yield. Yields from such varieties average 800 kg/ha of dry pods yet yields of 3,000kg/ha have been achieved from on-station plots. Yields per hectare are low, because of a combination of factors such as unreliable rains, mostly non-irrigated cultures, traditional small-scale farming with little mechanization, outbreaks of pest infestations and diseases, the use of low-yielding seed varieties and increased and/or continued cultivation on marginal land. Political instability and the frequently unsupportive oilseed policies have also played their role in low groundnut productivity. Therefore, there is excellent potential for yield improvement. Research efforts have, since the 1920s, endeavored to breed varieties that are high yielding, resistant to major pests and diseases, tolerant to drought, high in oil content and have a short to medium maturity period, as well as to develop appropriate production packages. These efforts have resulted in the release of 14 varieties, the most recent being Igola-1, Serenut 1R, Serenut 2T, Serenut 3R and Serenut 4T. These varieties have helped to alleviate some of the production problems listed above. However, the market and field stability of those varieties, in light of emerging stresses, calls for continuous research while at the same time keeping crop improvement, quality and safety linked to practical applications. Emerging issues like aflatoxin, leafminers and biotechnology need to be addressed and incorporated into the research agenda. Deployment of novel breeding approaches like molecular breeding to complement conventional breeding would increase the efficiency of cultivar development. Additionally, to maintain or increase market share, producers and exporters need to adapt the type of groundnuts being cultivated to consumer requirements. Key words: Arachis, Breeding NaSARRI, Uganda,
Kinetic characterization of glucose aerodehydrogenase from Aspergillus niger EMS-150-F after optimizing the dose of mutagen for enhanced production of enzyme

In the present study enhanced production of glucose aerodehydrogenase from Aspergillus niger has been achieved after optimizing the dose of chemical mutagen ethyl methane sulfonate (EMS) that has not been reported earlier. Different doses of mutagen were applied and a strain was developed basing upon the best production. The selected strain Aspergillus niger EMS-150-F was optimized for nutrient requirements in order to produce enzyme through fermentation and the results showed the best yield at 2% corn steep liquor (CSL), 36 hours fermentation time, pH 5, 30°C temperature, 0.3% KH2PO4, 0.3% urea and 0.06% CaCO3. The enzyme was then purified and resulted in 57.88 fold purification with 52.12% recovery. On kinetic characterization, the enzyme showed optimum activity at pH 6 and temperature 30°C. The Michaelis-Menton constants (Km, Vmax, Kcat and Kcat/Km) were 20 mM, 45.87 U mL−1, 1118.81 s−1 and 55.94 s−1 mM−1, respectively. The enzyme was found to be thermaly stable and the enthalpy and free energy showed an increase with increase in temperature and ΔS* was highly negative proving the enzyme from A. niger EMS-150-F resistant to temperature and showing a very little disorderliness.
Bifurcation analysis in models of tumor and immune system interactions

The purpose of this paper is to present qualitative and bifurcation analysis near the degenerate equilibrium in models of interactions between lymphocyte cells and solid tumor and to understand the development of tumor growth. Theoretical analysis shows that these cancer models can exhibit Bogdanov-Takens bifurcation under sufficiently small perturbation of the system parameters whether it is vascularized or not. Periodic oscillation behavior and coexistence of the immune system and the tumor in the host are found to be influenced significantly by the choice of bifurcation parameters. It is also confirmed that bifurcations of codimension higher than 2 cannot occur at this equilibrium in both cases. The analytic bifurcation diagrams and numerical simulations are given. Some anomalous properties are discovered from comparing the vascularized case with the avascular case.
Minimizing Latency of Critical Traffic through SDN

Internet of Things becomes one of the main trends in development of future networks. It introduces new challenges to network operator, like exponential growth of the traffic, large amount of network nodes to manage or variety of services. Some IoT services include the control of critical processes such as remote manipulation of a robotic arm in tele-surgery. In such processes, keeping very low end-to-end latency in the communication is crucial. The critical traffic generated becomes important and thus it needs special treatment, especially in situations when latency may be affected (e.g. network congestion or network node malfunctioning). In this work we analyzed what are the technical possibilities to manage traffic delay for important data. We selected Software Defined Networking technology to manage the end-to-end IoT traffic. An implementation based on OpenDayLight SDN controller allowed to build a solution that is able to identify in real-time the routing path with minimum delay and to route the important data traffic to this path. In order to monitor the latency, a probe packet is sent over each path and its trip time is measured. Simulations based on changes of the physical delay on links forming paths confirmed the effectiveness of our approach. They also showed the conditions, where the solution can be applied.
In Vivo Responses Age-Related Defects in CD4 T Cell Inflammatory Cytokines Overcome

Age-related decreases in immune function are thought to contribute to the reduced efﬁcacy of vaccinations seen in elderly populations. Our previous in vitro studies demonstrated that naive CD4 T cells from aged TCR-transgenic mice proliferate less than young cells and generate poorly functioning effectors due to decreased IL-2 production. In this current study, we show that this age-related defect in CD4 T cell response also occurs in vivo and that it is correlated with reduced NF- (cid:1) B activation. After transfer to young hosts, CD4 T cells from aged transgenic mice proliferate less and produce reduced levels of IL-2 upon immunization with Ag and alum. Introducing a combination of the inﬂammatory cytokines TNF- (cid:2) , IL-1, and IL-6, or the use of an adjuvant such as CFA that induces these cytokines, markedly enhanced responses of these aged CD4 T cells, so that they proliferated and produced IL-2 similar to young cells. This enhancement is correlated with the enhanced activation of the transcription factor NF- (cid:1) B in aged cells. We suggest that induction of inﬂammatory cytokines via adjuvants may enhance the efﬁcacy of vaccinations in elderly populations. The Journal of Immunology, 2004, 172: 5194–5199.
Peroxisome proliferator‐activated receptors in tumorigenesis: Targets of tumour promotion and treatment

The peroxisome proliferator‐activated receptors (PPAR) are ligand‐activated transcription factors. There are three genes that code for the PPAR isoforms: PPARα, PPARβ and PPARγ. In the present review, studies characterizing the various PPAR isoforms are discussed. Peroxisome proliferator‐activated receptor α has been implicated in the lipid‐lowering effects of the fibrate drugs. Peroxisome proliferator‐activated receptor γ has a clear role in adipocyte differentiation and is therapeutically targeted by the thiazolidinedione drugs for the treatment of type II diabetes. The physiological role of PPARβ is less well understood but, as described in the present review, recent studies have implicated it with a role in colon cancer. In the present review, particular attention is focused on the role of PPAR in the regulation of expression of proteins associated with cell cycle control and tumorigenesis.
Adenosine Stimulates the Migration of Human Endothelial Progenitor Cells. Role ofCXCR4 and MicroRNA-150

Background Administration of endothelial progenitor cells (EPC) represents a promising option to regenerate the heart after myocardial infarction, but is limited because of low recruitment and engraftment in the myocardium. Mobilization and migration of EPC are mainly controlled by stromal cell-derived factor 1α (SDF-1α) and its receptor CXCR4. We hypothesized that adenosine, a cardioprotective molecule, may improve the recruitment of EPC to the heart. Methods EPC were obtained from peripheral blood mononuclear cells of healthy volunteers. Expression of chemokines and their receptors was evaluated using microarrays, quantitative PCR, and flow cytometry. A Boyden chamber assay was used to assess chemotaxis. Recruitment of EPC to the infarcted heart was evaluated in rats after permanent occlusion of the left anterior descending coronary artery. Results Microarray analysis revealed that adenosine modulates the expression of several members of the chemokine family in EPC. Among these, CXCR4 was up-regulated by adenosine, and this result was confirmed by quantitative PCR (3-fold increase, P<0.001). CXCR4 expression at the cell surface was also increased. This effect involved the A2B receptor. Pretreatment of EPC with adenosine amplified their migration towards recombinant SDF-1α or conditioned medium from cardiac fibroblasts. Both effects were abolished by CXCR4 blocking antibodies. Adenosine also increased CXCR4 under ischemic conditions, and decreased miR-150 expression. Binding of miR-150 to the 3′ untranslated region of CXCR4 was verified by luciferase assay. Addition of pre-miR-150 blunted the effect of adenosine on CXCR4. Administration of adenosine to rats after induction of myocardial infarction stimulated EPC recruitment to the heart and enhanced angiogenesis. Conclusion Adenosine increases the migration of EPC. The mechanism involves A2B receptor activation, decreased expression of miR-150 and increased expression of CXCR4. These results suggest that adenosine may be used to enhance the capacity of EPC to revascularize the ischemic heart.
Alexithymia predicts loss chasing for people at risk for problem gambling

Background and aims The aim of this research was to investigate the relationship between alexithymia and loss-chasing behavior in people at risk and not at risk for problem gambling. Methods An opportunity sample of 58 (50 males and 8 females) participants completed the Problem Gambling Severity Index and the Toronto Alexithymia Scale (TAS-20). They then completed the Cambridge Gambling Task from which a measure of loss-chasing behavior was derived. Results Alexithymia and problem gambling risk were significantly positively correlated. Subgroups of non-alexithymic and at or near caseness for alexithymia by low risk and at risk for problem gambling were identified. The results show a clear difference for loss-chasing behavior for the two alexithymia conditions, but there was no evidence that low and at-risk problem gamblers were more likely to loss chase. The emotion-processing components of the TAS-20 were shown to correlate with loss chasing. Discussion and conclusion These findings suggest that loss-chasing behavior may be particularly prevalent in a subgroup of problem gamblers those who are high in alexithymia.
De-Europeanisation of Civil Society and Public Debates in Turkey: The Kurdish Question Revisited

Abstract This study discusses the dynamics of de-Europeanisation and the changing impact of Europe on the politically mobilised civil society involved in the public debates concerning Turkey’s Kurdish question. The article first critically assesses how and in what ways the legal and constitutional reforms on the freedom of assembly required by the European Union (EU) changed the political structure in which civil society organisations (CSOs) operate in Turkey. It then examines the views of CSOs on the potential roles and limitations of the EU in the Kurdish question and the peace process which lasted between March 2013 and July 2015. It also delineates the reasons why the political context of Europeanisation is not seen as instrumental by these CSOs to framing and justifying their arguments.
A New Efficient Approach to Store Data in a Cloud Server

The present study aims at introducing a new efficient approach to store data in a cloud server. The approach is applicable to a group of annotated text files, which are supported by Microsoft Word (docx, doc, pdf, etc.). Instead of the original document, an intermediate file is produced and saved as a Microsoft Excel document for each user. The file contains edited information including highlighted and underlined parts, comments, etc. Whenever a user wants to access his/her document, the intermediate file is attached to the original document and appeared to him/her. The experimental results of 50 users show that a 2.5MB file and in total, 126MB files can be reduced in size to 3MB. It means a large amount of disk space can be saved by the cloud server.
Recent developments in high-level synthesis

We survey recent developments in high level synthesis technology for VLSI design. The need for higher-level design automation tools are discussed first. We then describe some basic techniques for various subtasks of high-level synthesis. Techniques that have been proposed in the past few years (since 1994) for various subtasks of high-level synthesis are surveyed. We also survey some new synthesis objectives including testability, power efficiency, and reliability.
SifA permits survival and replication of Salmonella typhimurium in murine macrophages

SifA was originally identified as a virulence factor required for formation of Salmonella‐induced filaments (Sifs), elongated tubules rich in lysosomal glycoproteins that extend from the Salmonella‐containing vacuole in infected epithelial cells. Here, we demonstrate that deletion mutants of ssaR, a component of the SPI‐2 type III secretion system, do not form Sifs in HeLa epithelial cells. This suggests that SifA is a translocated effector of this system, acting within host cells to form Sifs. In support of this hypothesis, transfection of HeLa cells with a vector encoding SifA fused to the green fluorescent protein caused extensive vacuolation of LAMP‐1‐positive compartments. Filamentous tubules that closely resembled Sifs were also observed in transfected cells, demonstrating that SifA is sufficient to initiate alteration of host cell endosomal structures. ΔsifA mutants were impaired in their ability to survive/replicate in RAW 264.7 murine macrophages, a phenotype similar to ssaR mutants. Our findings suggest that SifA is an effector of the SPI‐2 type III secretion system and allows colonization of murine macrophages, the host niche exploited during systemic phases of disease in these animals. A family of SifA‐related proteins and their importance to Salmonella pathogenesis is also discussed.
Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin

Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker's presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3+, CD8+, and CD68+ cells at the DEJ, and CD20+ and CD68+ cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process.
Paedophilia: A Review of the Evidence*

Objective: The aim of this study was to critically review the literature concerning the nature and prevalence of paedophilia. Method: The literature of the past 30 years was examined in relation to the author's clinical experience and with emphasis on methodologically appropriate empirical studies. Results: Concern and reporting of child-adult sexual activity has increased markedly in the last decade, although its prevalence has not increased at least since the 1960s. The prevalence in women can be as high as 60%, depending on the definition and method of enquiry used, and female compared to male victims report more negative effects, although a percentage of both men and women report the experience as positive. Validation of effects requires multivariate statistical analysis. Current pro-active procedures to identify paedophiles detect those who victimise boys but do not detect the much greater number of paedophiles who victimise girls. Perpetrators are known to the majority of their female and male victims, and those reported are almost all male; most boys do not consider their prepubertal experiences with older women abusive. Relapse prevention, the current most popular treatment, has been shown to be ineffective for incarcerated child molesters. Conclusions: Child-adult sexual activity should be opposed as an infringement of children's rights rather than requiring a false belief that it is invariably harmful; whether it should be mandatory for therapists to report it requires examination. Scientifically appropriate evaluation should be an essential component of current treatment programs.
Presentation of China in online West European media: More fairness and accuracy required

This article focuses on the presentation of China in online West European media and examines reporting on China in British, Dutch and Belgian online newspapers and weblogs. Through concrete case studies he uncovers the reasons for the predominantly negative coverage of China-related topics in the above-mentioned sources, in particular, to explain the inaccuracy, bias and partisanship. It further studies the complex relationship between journalism and activism and presents a number of analyses that shed light upon the process of construction of an unfavourable opinion about China that the attitudes of the media industry have contributed to. By showing the weaknesses in the coverage of China of the West European media such as distortion of the information or self-censorship, the article criticizes the absence of objectivity in reporting, and proposes a number of measures that both European and Chinese journalists could take so as to overcome the imbalance and prejudice in reporting and move towards more fairness and accuracy.
The problem of speaking for others

dian women. She writes them in first person and assumes a Native identity. At the 1988 International Feminist Book Fair in Montreal a group of Native Canadian writers decided to ask Cameron to, in their words, "move over" on the grounds that her writings are disempowering for Native authors. She agrees. ' 2. After the 1989 elections in Panama are overturned by Manuel Noriega, President Bush of the United States declares in a public address that Noriega's actions constitute an "outrageous fraud" and that "the voice of the Panamanian people has spoken. " "The Panamanian people," he tells us, "want democracy and not tyranny, and want Noriega out. " He proceeds to plan the invasion of Panama.