Miracle-dz/UniFAR-FTUs huggingface dataset:
Phosphorylation of Calcineurin B-like (CBL) Calcium Sensor Proteins by Their CBL-interacting Protein Kinases (CIPKs) Is Required for Full Activity of CBL-CIPK Complexes toward Their Target Proteins*

Calcineurin B-like proteins (CBLs) represent a family of calcium sensor proteins that interact with a group of serine/threonine kinases designated as CBL-interacting protein kinases (CIPKs). CBL-CIPK complexes are crucially involved in relaying plant responses to many environmental signals and in regulating ion fluxes. However, the biochemical characterization of CBL-CIPK complexes has so far been hampered by low activities of recombinant CIPKs. Here, we report on an efficient wheat germ extract-based in vitro transcription/translation protocol that yields active full-length wild-type CIPK proteins. We identified a conserved serine residue within the C terminus of CBLs as being phosphorylated by their interacting CIPKs. Remarkably, our studies revealed that CIPK-dependent CBL phosphorylation is strictly dependent on CBL-CIPK interaction via the CIPK NAF domain. The phosphorylation status of CBLs does not appear to influence the stability, localization, or CIPK interaction of these calcium sensor proteins in general. However, proper phosphorylation of CBL1 is absolutely required for the in vivo activation of the AKT1 K+ channel by CBL1-CIPK23 and CBL9-CIPK23 complexes in oocytes. Moreover, we show that by combining CBL1, CIPK23, and AKT1, we can faithfully reconstitute CBL-dependent enhancement of phosphorylation of target proteins by CIPKs in vitro. In addition, we report that phosphorylation of CBL1 by CIPK23 is also required for the CBL1-dependent enhancement of CIPK23 activity toward its substrate. Together, these data identify a novel general regulatory mechanism of CBL-CIPK complexes in that CBL phosphorylation at their flexible C terminus likely provokes conformational changes that enhance specificity and activity of CBL-CIPK complexes toward their target proteins.
Cultural and demographic correlates of self‐reported guardedness among East Asian immigrants in the US

The study examined guarded self‐disclosure or reticence to reveal personal information about oneself to others among East Asian immigrants in the United States, in terms of cultural (ethnic identity, self‐construal, and acculturation) and demographic (gender, age, number of years in the US, and English fluency) variables. Norms regarding appropriate amount, content, and type of self‐disclosure differ noticeably between Asian and American cultures. In general, Asians have been found to be more guarded in their self‐disclosure than their American counterparts. Such differences may hinder the formation of relationships between Asian immigrants and Americans. Thus, it is important for psychologists to examine culture‐specific and non‐culture‐specific correlates of guarded self‐disclosure among Asian immigrants in the United States. However, to date, there has been a dearth of studies examining this topic. This paucity of research is somewhat surprising given the large number of Asian immigrants in the United .
Minimization of memory access overhead for multidimensional DSP applications via multilevel partitioning and scheduling

Massive uniform nested loops are broadly used in multidimensional digital signal processing (DSP) applications. Due to the large amount of data handled by such applications, the optimization of data accesses by fully utilizing the local memory and minimizing communication overhead is important in order to improve the overall system performance. Most of the traditional partition strategies do not consider the effect of data access on the computational performance. In this paper, a multilevel partitioning method, based on a static data scheduling technique known as carrot-hole data scheduling, is proposed to control the data traffic between different levels of memory. Based on this data schedule, optimal partition vector, scheduling vector and the partition size are chosen in such a way to minimize communication overhead. Nonhomogeneous size partitions are the final result of the partition scheme which produces a significant performance improvement. Experiments show that by using this technique, local memory misses are significantly reduced as compared to results obtained from traditional methods. This method can be used in application specific DSP system design and compiler for DSP processors.
Learning from Errors: Cognition, Emotions and Safety Culture in the Italian Air Force

Learning from errors is essential to ensuring organizational safety and improving levels of performance. We consider the interaction between cognition, emotions, and safety culture in the context of a field study on learning from errors in the Italian Air Force. We find that errors often stem from sequential action chains that are concealed in habitual behavior and that become visible only when unforeseen circumstances occur. Furthermore, cognitive appraisal of risky situations triggers emotions of variable intensity that, when rationalized retrospectively, promote the internalization of lessons learned. Finally, cognitive and emotional experiences of errors are grounded in the broader safety culture of an organization, which provides a supportive context for error reporting and encourages the sharing of information and knowledge about error experiences. The analysis further suggests that cognition, emotion, and safety culture interact through sensemaking processes that inform the construction of errors and affect learning outcomes.
Pulmonary function in acute myocardial infarction.

cases of self-poisoning coming to hospital and not just with those cases somehow selected for psychiatric investigation. Those with no psychiatric illness tended to be younger ; those with organic illnesses were generally old. Apart from this the relation between diagnosis and age was not close. The principal diagnosis was related to the index of endangering life (Table VIII). The acts of patients with a formal psychiatric illness (excluding character disorder) were more life-endangering than those of patients with personality abnormality or those of patients without a psychiatric illness. Yet the majority of acts of even the more seriously ill people were clearly pointed towards survival.
'Between Democracy and Expertise? Citizens' Participation and Environmental Integrated Assessment in Venice (Italy) and St.Helens (UK)

Citizens' participation in integrated assessment of climate change highlights the uneasy relationship between democracy and expert knowledges as they have been generally understood in the West. Based on empirical material gathered through in-depth groups in Venice (Italy) and citizens' panels in St. Helens (UK), the paper explores: (1) some of the challenges and opportunities of qualitative and comparative research on climate change; and (2) the similarities and differences in citizens’ environmental assessment in two European cities facing economic, urban and social re-structuring. The authors research reveals a general willingness to participate, albeit conditional, despite initial scepticism and interrogation about the objectives. During the process, participants in both cities show: (a) a broad contextual understanding of uncertainties and complexities; (b) how important interpersonal skills between lay and expert publics are; and (c) that precaution should generally apply in the case of uncertainties.
Effects of Fluoride on the Interactions between Amelogenin and Apatite Crystals

Fluorosed enamel is more porous and less mineralized, possibly related to altered amelogenin-modulated crystal growth. The purpose of this study was to examine the role of fluoride in interactions between amelogenin and apatite crystals. Recombinant human amelogenin (rh174) was bound to carbonated hydroxyapatite containing various amounts of fluoride, and analyzed by protein assay, SDS PAGE, and AFM. Interactions between rh174 and fluoride were assayed by isothermal titration calorimetry (ITC). The initial binding rate of rh174, as well as total amount of rh174 bound to fluoride-containing carbonated hydroxyapatite, was greater than that in the control carbonated hydroxyapatite. Fluoride in solution at physiologic (5.3 micromolar, or 0.1 ppm) concentrations showed no significant effect on binding, but higher fluoride levels significantly decreased protein binding. ITC showed no interactions between fluoride and rh174. These results suggest that fluoride incorporation into the crystal lattice alters the crystal surface to enhance amelogenin binding, with no direct interactions between fluoride and amelogenin.
Global Resilience of Tropical Forest and Savanna to Critical Transitions

Tree distributions across continents indicate three distinct stable states in tree cover―forest, savanna, and treeless. It has been suggested that tropical forest and savanna could represent alternative stable states, implying critical transitions at tipping points in response to altered climate or other drivers. So far, evidence for this idea has remained elusive, and integrated climate models assume smooth vegetation responses. We analyzed data on the distribution of tree cover in Africa, Australia, and South America to reveal strong evidence for the existence of three distinct attractors: forest, savanna, and a treeless state. Empirical reconstruction of the basins of attraction indicates that the resilience of the states varies in a universal way with precipitation. These results allow the identification of regions where forest or savanna may most easily tip into an alternative state, and they pave the way to a new generation of coupled climate models.
The micro–macro link in deliberative polling: science or politics?

In this article, we critically examine the question of how to link the ‘micro’ of deliberative mini-publics with the ‘macro’ of the democratic system. To explore this puzzle, we relate to EuroPolis, a transnational deliberative experiment that took place one week ahead of the 2009 European Parliamentary elections. The main argument is that although the scientific design of deliberative polls is a necessary condition for the fulfilment of the criteria for equal participation and informed opinion-making of selected citizens (the micro-dimension), this does not necessarily translate into a democratically representative and legitimate proxy for the broader political constituency (the macro-dimension). This problem is potentially exacerbated in deliberative settings that cut across domestic political cultures and nationalized public spheres.
The Impact of Prison Growth on Homicide

The major crime-reduction strategy in this country is to imprison more and more criminals. Past studies of its effectiveness in reducing homicide have produced wildly divergent results. The authors analyze the homicide-prison relationship with a time series over 1930-1994, finding that a 10% increase in prison population is associated with roughly 13% fewer homicides. The authors also studied assault and robbery, two crime types linked to homicide, and again found negative associations with prison population.
Constructive Role of Interorganizational Conflict.

Conflict between business organizations may have constructive consequences. This article reports on conflict within the automobile distribution system based on a two-year exploratory study, with the relationship between General Motors and its dealers as the primary example. It distinguishes between constructive and destructive conflict by drawing on five premises about intergroup conflict from sociology and applying them to manufacturer-dealer relations. The economic, organizational, and political conditions that encourage a systematic and equitable resolution of conflict are considered.
Contact process on a graph with communities

We are interested in the spread of an epidemic between two communities that have higher connectivity within than between them. We model the two communities as independent Erdos-Renyi random graphs, each with n vertices and edge probability p = n^{a-1} (0 1 then the contact process on the Erdos-Renyi random graph is supercritical, and we show that it survives for exponentially long. Further, let \tau be the time to infect a positive fraction of vertices in the second community when the infection starts from a single vertex in the first community. We show that on the event that the contact process survives exponentially long, \tau |B|/(np) converges in distribution to an exponential random variable with a specified rate. These results generalize to a graph with N communities.
A Wernerian Developmental Analysis of Children's Descriptions of Siblings

constructs. -Constructs which included references to abstract qualities or attributes of the older sibling, for example, "He's very nice and kind," or "She's the worst pest. " These categories were arranged in a postulated developmental sequence (concrete to abstract, egocentric to nonegocentric). A construct was assigned to a higher-level category if doubt existed about to which of two categories it should be assigned. Only those relevant constructs indicative of the described person's interpersonal qualities were scored. Those relating to physical appearance and abilities were excluded from the analysis. Reliability of coding the constructs was determined by selecting 30 protocols from each age group for scoring the total number of the four types of constructs used to describe each of the four sibling types (likeddisliked brother, liked-disliked sister). Reliability was analyzed by counting the number of scores each of the two raters identically assigned to each category and by computing the correlation between the two raters' assignments for each category. Analyses indicated that the two raters were in agreement on 85% of their total assignments by category, and the mean within-cell correlation between the
Beyond birth-weight: early growth and adolescent blood pressure in a Peruvian population

Background. Longitudinal investigations into the origins of adult essential hypertension have found elevated blood pressure in children to accurately track into adulthood, however the direct causes of essential hypertension in adolescence and adulthood remains unclear. Methods. We revisited 152 Peruvian adolescents from a birth cohort tracked from 0 to 30 months of age, and evaluated growth via monthly anthropometric measurements between 1995 and 1998, and obtained anthropometric and blood pressure measurements 11–14 years later. We used multivariable regression models to study the effects of infantile and childhood growth trends on blood pressure and central obesity in early adolescence. Results. In regression models adjusted for interim changes in weight and height, each 0.1 SD increase in weight for length from 0 to 5 months of age, and 1 SD increase from 6 to 30 months of age, was associated with decreased adolescent systolic blood pressure by 1.3 mm Hg (95% CI −2.4 to −0.1) and 2.5 mm Hg (95% CI −4.9 to 0.0), and decreased waist circumference by 0.6 (95% CI −1.1 to 0.0) and 1.2 cm (95% CI −2.3 to −0.1), respectively. Growth in infancy and early childhood was not significantly associated with adolescent waist-to-hip ratio. Conclusions. Rapid compensatory growth in early life has been posited to increase the risk of long-term cardiovascular morbidities such that nutritional interventions may do more harm than good. However, we found increased weight growth during infancy and early childhood to be associated with decreased systolic blood pressure and central adiposity in adolescence.
Effect of alpha-pinene on nuclear translocation of NF-kappa B in THP-1 cells.

AIM To study the effects of alpha-pinene on nuclear translocation of nuclear factor-kappa B (NF-kappa B) and the expression of the inhibitor of NF-kappa B (I kappa B alpha) in human monocyte THP-1 cell line. METHODS THP-1 cells were incubated with alpha-pinene (1, 10, and 100 mg/L, for 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg/L, 30 min). The location of NF-kappa B p65 subunit (NF-kappa B/p65) in THP-1 cells was detected by immunofluorescence and laser scanning confocal microscope (LSCM). The expression of NF-kappa B/p65 in nuclei and that of I kappa B alpha in cytoplasm were measured by Western-blot analysis. RESULTS The majority of FITC-labelled NF-kappa B/p65 was located in the nuclei being stimulated with LPS. Whereas, no such fluorescence was seen in the nuclei of the groups pretreated with alpha-pinene or control cells. alpha-Pinene pretreatment decreased the NF-kappa B/p65 nuclear translocation in LPS-stimulated THP-1 cells, and this effect was dose-dependent, but there was no reaction in LPS-unstimulated THP-1 cells. alpha-Pinene pretreatment increased I kappa B alpha protein level in cytoplasm, compared with that in LPS-stimulated THP-1 cells. CONCLUSION In a dose-related fashion, alpha-pinene inhibits the nuclear translocation of NF-kappa B induced by LPS in THP-1 cells, and this effect is partly due to the upregulation of I kappa B alpha expression.
Effect of Consumption Emotion on Hotel and Resort Spa Experience

ABSTRACT Very few studies focus on the behavior of hotel and resort spa customers in China. Hence, this research proposes a model for testing the impact of spa service quality, positive and negative consumption emotions, and hedonic and utilitarian perceived value on the behavioral intentions to visit a spa. The valid sample comprised 487 customers of five-star hotel/resort spas in China. Data were obtained from the self-administered survey answered by respondents constituting the sample. This study identified the vital function of consumption emotion, especially positive emotion dimension. The results indicated the mediating influence of positive emotion on connecting service quality and creating the perceived value of consumers as well as behavioral intention in the hotel and resort spa context.
Structural characterization of PNA-DNA duplexes by NMR. Evidence for DNA in a B-like conformation.

The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
Commentary: Brain, Mind, World: Predictive Coding, Neo-Kantianism, and Transcendental Idealism

Zahavi claims that the predictive processing (PP) approach supports a radical neurorepresentationalism, “according to which the content of our conscious experiences is a neural construct, a brain-generated simulation” (Zahavi, 2017, p. 1). He claims that this is because, on the one hand, spontaneous human cognition becomes equated with the model of cognition developed by sciences, and on the other, the representational position challenges the belief concerning the objectivity of the world of experience. It is of key importance, therefore, to answer the question posed in the article: “Which position is the best able to accommodate our natural inclination for realism: Contemporary neuro-representationalism or Husserl’s transcendental idealism? ” (Zahavi, 2017, p. 2). The author defends the answer that refers back to Husserl’s thought. In this commentary, I will demonstrate that (1) the understanding of PP as suggested by Zahavi is not adequate for the different accounts offered in the literature; (2) we can identify such PP conceptions which do not continue neo-Kantism; and (3) the problem of the normativity of prediction enables us to treat PP and Husserl’s phenomenology as complementary rather than antagonistic approaches.
Environmental Tobacco Smoke and Pregnancy Outcome

Background: Recent reviews conclude that environmental tobacco smoke (ETS) leads to diminished birth weight. However, the threshold and magnitude of that effect is uncertain. We aimed to determine the magnitude and shape of the relations between ETS and various adverse pregnancy outcomes using a highly sensitive biochemical assay. Methods: Maternal serum specimens were collected from more than 3000 women enrolled in California's prenatal screening program in 1992 and analyzed for cotinine. Information on pregnancy outcomes was obtained from live birth/fetal death records and hospital questionnaires. We conducted analyses on 2777 woman–live birth pairs and 19 woman–fetal death pairs in which the mother was presumed to be a nonsmoker (midtrimester cotinine levels ≤10 ng/mL). Results: In multiple logistic regression analyses, the odds ratios of fetal death, preterm delivery, and term-low birth weight were 3.4, 1.8, and 1.8, respectively, in the highest cotinine quintile (0.236–10 ng/mL), compared with the lowest quintile (<0.026 ng/mL). In adjusted linear models, there was a linear dose-dependent effect of log cotinine on mean birth weight (−109 g) and mean infant length (−0.84 cm) over the range of cotinine values. Linear relations were not found with respect to infant head circumference or the ratio of brain weight to body weight. Infant's body mass index declined with exposures above approximately 0.5 ng/mL cotinine. We estimated that ETS levels at or above 0.05 ng/mL (experienced by 62% of the study population) accounted for 12% of all adverse outcomes. Conclusions: ETS exposure in pregnant women adversely affects pregnancy by increasing fetal mortality and preterm delivery at higher exposure levels and slowing fetal growth across all levels of ETS exposure.
Freedom of Expression and the Marketplace of Ideas Concept From Milton to Jefferson

Jeffery A. Smith is a doctoral student in the School of Journalism and Mass Communication at the University of Wisconsin&mdash;Madison. His present research is in the history and law of eighteenth-century American journalism. Nations do not give a priority to the process of discerning new truths. A crisis may pr~~~pt the expression of fresh views of reality, but stability-with its attendant myths-is valued over innovation. Uncommon is the theorist like ilt&reg;~ or Jefferson who the of the s~ ch for truth and envisions akin to permanent revolution. More typical is the intellectual strain of Plato, and Lenin which the use of deceit and regimentation to obtain the ends of society. the faith associated °th the Enlightenment-that error could and should be eliminated and that societies would progress as a result-was never
Feeling with the mind's eye

MENTAL imagery is thought to play a key role in certain aspects of visual perception and to depend on neural activity in visual cortex. We asked whether tactile discrimination of grating orientation, which appears to involve visual mental imagery, recruits visual cortical areas. H215O positron emission tomography was performed in humans during presentation of gratings to the right index fingerpad. Selective attention to grating orientation significantly increased regional cerebral blood flow, relative to a control task involving selective attention to grating dimensions, in a region located in left parieto-occipital cortex. We propose that this activation reflects the use of imagery-related visuo-spatial processes to enable the tactile discrimination of orientation.