Miracle-dz/UniFAR-FTUs huggingface dataset:
Tissue-resident natural killer (NK) cells are cell lineages distinct from thymic and conventional splenic NK cells

Natural killer (NK) cells belong to the innate immune system; they can control virus infections and developing tumors by cytotoxicity and producing inflammatory cytokines. Most studies of mouse NK cells, however, have focused on conventional NK (cNK) cells in the spleen. Recently, we described two populations of liver NK cells, tissue-resident NK (trNK) cells and those resembling splenic cNK cells. However, their lineage relationship was unclear; trNK cells could be developing cNK cells, related to thymic NK cells, or a lineage distinct from both cNK and thymic NK cells. Herein we used detailed transcriptomic, flow cytometric, and functional analysis and transcription factor-deficient mice to determine that liver trNK cells form a distinct lineage from cNK and thymic NK cells. Taken together with analysis of trNK cells in other tissues, there are at least four distinct lineages of NK cells: cNK, thymic, liver (and skin) trNK, and uterine trNK cells. DOI: http://dx.doi. org/10.7554/eLife.01659. 001
The Labilityof EstrogenReceptor : Correlationof EstrogenBindingand Immunoreactivity

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On the Physics of the Warm Water Volume and El Niño/La Niña Predictability

Previous work has shown that warm water volume (WWV), usually defined as the volume of equatorial Pacific warm water above the 20°C isotherm between 5°S and 5°N, leads El Niño. In contrast to previous discharge–recharge oscillator theory, here it is shown that anomalous zonal flow acceleration right at the equator and the movement of the equatorial warm pool are crucial to understanding WWV–El Niño dynamics and the ability of WWV to predict ENSO. Specifically, after westerly equatorial wind anomalies in a coupled ocean–atmosphere instability push the warm pool eastward during El Niño, the westerly anomalies follow the warmest water south of the equator in the Southern Hemisphere summer in December–February. With the wind forcing that causes El Niño in the eastern Pacific removed, the eastern equatorial Pacific sea level and thermocline anomalies decrease. Through long Rossby wave dynamics this decrease results in an anomalous westward equatorial flow that tends to push the warm pool westward and often results in the generation of a La Niña during March–June. The anomalously negative eastern equatorial Pacific sea level typically does not change as much during La Niña, the negative feedback is not as strong, and El Niños tend to not follow La Niñas the next year. This El Niño/La Niña asymmetry is seen in the WWV/El Niño phase diagram and decreased predictability during “La Niña–like” decades.
T-STL : Track-based Shingled Translation Layer for Autonomous SWD ∗

Shingled Magnetic Recording (SMR) is a promising technique for increasing hard disk drive (HDD) capacity by overlapping adjacent tracks, which enforces writes to be performed in a sequential manner in order not to destroy the valid data in the following neighboring tracks. In this paper we introduce T-STL, a Track-based Shingled Translation Layer for autonomous Shingled Write Disks (SWDs). T-STL minimizes the write amplification overhead by utilizing two unique properties of SWDs to handle workloads differently according to the SWD space utilization. First, when SWD space utilization is less than 50%, T-STL turns the SWD into a HDD-like device by using every other track 1. The unused tracks work as safety gaps to avoid data overwriting. Therefore, in-place updates are possible in this situation. The second property is a track-based mapping instead of a typical block-based mapping. When SWD utilization is over 50%, tracks that do not allow in-place updates are updated in a copy-on-write manner as out-of-place updates. In a track-based mapping, when a track is invalidated, it becomes free right away and can be immediately reused as long as the next track is free too, without triggering an explicit garbage collection (GC) operation. Only when the free SWD space becomes extremely fragmented, an explicit on-demand GC operation needs to be invoked to create big contiguous free space by migrating some valid tracks. Efficient track-level mapping and space management schemes are also designed to fully utilize these two properties. We implement the T-STL scheme and compare it with a regular HDD, an existing out-of-place update SWD design and an in-place update SWD. The experiments with several realistic traces and one synthetic trace demonstrate that the T-STL scheme can perform much better than the existing SWD designs and even nearly as good as regular HDDs when SWD space usage is less than 50%. ∗This work was partially supported by NSF Awards: 1217569 and 1439622. 1We assume the write head is 2-track wide in our discussions. However, our schemes can be adapted for bigger write head width.
Treatment of hypotony maculopathy after trabeculectomy.

BACKGROUND AND OBJECTIVE To determine if resuturing of the scleral flap to increase IOP in the post-trabeculectomy eye is an effective treatment of hypotony maculopathy. PATIENTS AND METHODS The medical records of nine consecutive patients who developed visual acuity loss after trabeculectomy related to hypotony maculopathy were reviewed to determine factors that contribute to successful restoration of visual acuity. The average age of the patients was 50 years and average refractive error was -4.25 diopters (D). Mean Snellen visual acuity dropped from 20/25 to 20/200 after the onset of hypotony. RESULTS Six of nine eyes recovered visual acuity to within one line of initial level after treatment of the hypotony. Four of these six eyes underwent resuturing of the scleral flap to reverse the excess filtration within 6 months of the onset of hypotony maculopathy. Five of these six patients had final vision of 20/30 or better. The mean phakic preoperative refractive error in these six eyes was -2.25 D, compared with a mean refractive error of -8.50 D in the three eyes with persistent visual loss (P = 0.002). After reversal of the hypotony, the average peak intraocular pressure in the six eyes with visual recovery was 33 mm Hg, compared with an average peak IOP of 7 mm Hg in the three eyes with persistent visual loss. (P = 0.015) CONCLUSION Although reversal of the hypotony maculopathy in six eyes was associated with a few weeks of higher than normal IOP, the final IOP was acceptably low. Five of six filtering blebs remained functional with a mean follow-up of 3 years. The final average IOP in the six eyes that were successfully treated was 12.7 mm Hg. In this series of patients, visual acuity was restored to eyes with hypotony maculopathy by increasing the IOP to higher than normal levels.
The Asymmetry of Galaxies: Physical Morphology for Nearby and High-Redshift Galaxies

We present a detailed study of rotational asymmetry in galaxies for both morphological and physical diagnostic purposes. An unambiguous method for computing asymmetry is developed, which is robust for both distant and nearby galaxies. By degrading real galaxy images, we test the reliability of this asymmetry measure over a range of observational conditions, e.g., spatial resolution and signal-to-noise ratio (S/N). Compared to previous methods, this new algorithm avoids the ambiguity associated with choosing a center by using a minimization method and successfully corrects for variations in S/N. There is, however, a strong relationship between the rotational asymmetry and physical resolution (distance at fixed spatial resolution): objects become more symmetric when less well-resolved. We further investigate asymmetry as a function of galactic radius and rotation. We find the asymmetry index has a strong radial dependence that differs vastly between Hubble types. As a result, a meaningful asymmetry index must be specified within a well-defined radius representative of the physical galaxy scale. We enumerate several viable alternatives, which exclude the use of isophotes. Asymmetry as a function of angle (Aϕ) is also a useful indicator of ellipticity and higher order azimuthal structure. In general, we show that the power of asymmetry as a morphological parameter lies in the strong correlation with B-V color for galaxies undergoing normal star formation spanning all Hubble types from ellipticals to irregular galaxies. The few interacting galaxies in our study do not fall on this asymmetry-color "fiducial sequence," as these galaxies are too asymmetric for their color. We suggest this fact can be used to distinguish between "normal" galaxies and galaxies undergoing an interaction or merger.
PERTURBATION THEORY RELOADED. II. NONLINEAR BIAS, BARYON ACOUSTIC OSCILLATIONS, AND MILLENNIUM SIMULATION IN REAL SPACE

We calculate the nonlinear galaxy power spectrum in real space, including nonlinear distortion of the baryon acoustic oscillations, using the standard third-order perturbation theory (PT). The calculation is based upon the assumption that the number density of galaxies is a local function of the underlying, nonlinear density field. The galaxy bias is allowed to be both nonlinear and stochastic. We show that the PT calculation agrees with the galaxy power spectrum estimated from the Millennium Simulation, in the weakly nonlinear regime (defined by the matter power spectrum) at high redshifts, 1 ⩽ z ⩽ 6. We also show that, once three free parameters characterizing galaxy bias are marginalized over, the PT power spectrum fit to the Millennium Simulation data yields unbiased estimates of the distance scale, D, to within the statistical error. This distance scale corresponds to the angular diameter distance, DA(z), and the expansion rate, H(z), in real galaxy surveys. Our results presented in this paper are still restricted to real space. The future work should include the effects of nonlinear redshift space distortion. Nevertheless, our results indicate that nonlinear galaxy bias in the weakly nonlinear regime at high redshifts is reasonably under control.
Perceptions of Leadership: An Analysis of College Students' Understandings of the Concept of Leadership

The purpose of this mixed-methods study was to examine how college students define the concept of leadership and to identify gender, racial, and age differences within these definitions. Participants were 1100 undergraduate students drawn from a national sample. Participants were asked to detail their definitions of leadership, which were analyzed using thematic content analysis. The definitional themes served as dependent variables in determining differences by gender, race, and age. Ten leadership themes describing the relationship between the leader and followers, leader characteristics, and leadership outcomes were identified, and the frequency of themes reflected fairly hierarchical perceptions of leadership. Findings by gender, race, and age emerged for nine of the ten themes. More contemporary understandings of leadership were demonstrated by women than by men, and the interaction of gender and age for three themes suggested more contemporary understandings of leadership for traditionally-aged students than non-traditionally aged students.
Histological Evaluation of Single and Double-visit Direct Pulp Capping with Different Materials on Sound Human Premolars: A Randomized Controlled Clinical Trial

Introduction: The aim of this study was to evaluate the clinical and histological status of the pulp in sound human premolars after direct pulp capping (DPC) with four different DPC methods/materials. Methods and Materials: This study was conducted on eight volunteers who had to extract four first premolars due to orthodontic treatment. Subsequent to tooth isolation, standardized class I occlusal cavities were prepared and the buccal pulp horns were exposed. Then four different protocols of DPC were applied randomly: group A (control); calcium hydroxide lining paste (Dycal), group B; ProRoot MTA (standard double-visit method), group C; ProRoot MTA (single-visit method) and group D; calcium hydroxide injectable paste (Multi-Cal). The cavities were then restored and the patients were put on a six-week clinical follow-up and by the end of this period the teeth were extracted for histological evaluation. Data were analyzed with the Kruskal Wallis test and the level of significance was set at 0.05. Results: In terms of clinical symptoms and formation of hard tissue bridge (HTB), no significant differences were found between groups A, B and C (P>0.05); however, group D’s results were significantly different as they exhibited minimal HTB formation and excessive sensitivity (P<0.05). Inflammation was significantly lower in group B (P>0.05). Conclusion: Application of MTA during a single-visit protocol of DPC was clinically and histologically as successful as the standard double-visit method but the routine use of Multi-Cal as pulp capping material is questionable and should be reconsidered.
Oral Administration of Gelatin Hydrolysate Reduces Clinical Signs of Canine Osteoarthritis in a Double-Blind, Placebo-Controlled Trial

Problem statement: There are indications that the intake of gelatin hydrolysate has a beneficial impact on the clinical signs of osteoarthritis in dogs. Data from a controlled trial were required to substantiate these indications. Approach: A double-blind, placebo-controlled trial with privately owned dogs was carried out to assess the efficacy of a preparation of gelatin hydrolysate in the treatment of osteoarthritis. With the use of a questionnaire, the clinical signs were evaluated by the owners. For a period of 8 weeks, the test dogs daily received 10 g of gelatin hydrolysate; as a placebo, soya protein isolate was used. The supplements were mixed with the diet; all dogs were fed on the same dry food. There were 15 dogs per treatment group. Results: The administration of gelatin hydrolysate significantly improved activity (vitality) and significantly reduced stiffness and lameness. Conclusion: Gelatin hydrolysate is commonly used as a component of human foods and is generally considered as safe. It is suggested that a dose of about 2.5% in a dry food would be beneficial for dogs with osteoarthritis.
Microparticle‐associated nucleic acids mediate trait dominance in cancer

Drug resistance is a major cause of cancer treatment failure, with multidrug resistance (MDR) being the most serious, whereby cancer cells display cross‐resistance to structurally and functionally unrelated drugs. MDR is caused by overexpression of the efflux transporters P‐glycoprotein (P‐gp) and multidrug resistance‐associated protein 1 (MRP1). These transporters act to maintain sublethal intracellular drug concentrations within the cancer cell, making the population treatment unresponsive. Recently, we discovered a novel nongenetic basis to MDR whereby microparticles (MPs) transfer P‐gp intercellularly from MDR donor cells to drug‐sensitive recipient cells. MPs isolated from MDR leukemia and breast cancer cells were cocultured with their drug‐sensitive counterparts. P‐gp transfer was assessed by direct immunolabeling, and acquired transcripts and regulatory microRNAs by quantitative real‐time PCR. We show that MDR MPs incorporate nucleic acids; MPs change recipient cells' transcriptional environment to reflect donor MDR phenotype, and distinct pathways exist among cancers of different origin that may be dependent on donor cells' ABCB1 overexpression. We demonstrate that this pathway exists for both hematological and nonhematological malignancies. By conferring MDR and “retemplating” the transcriptional landscape of recipient cells, MPs provide a novel pathway, having implications in the dissemination and acquisition of deleterious traits in clinical oncology. —Jaiswal, R. , Gong, J., Sambasivam, S. , Combes, V. , Mathys, J.‐M. , Davey, R. , Grau, G. E. R. , Bebawy, M. Microparticle‐associated nucleic acids mediate trait dominance in cancer. FASEB J. 26, 420–429 (2012). www.fasebj. org
Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and susceptibility to diabetic nephropathy in Chinese type 2 diabetic patients: a meta-analysis

Abstract The association between methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and diabetic nephropathy (DN) or diabetes mellitus (DM) risk has been widely reported, but the results are still debatable. To investigate the role of MTHFR C677T polymorphism on DM or DN, 13 separate studies in the Chinese population on the relation between MTHFR C677T polymorphism and DM or DN were analyzed by a meta-analysis. Five genetic models were used to estimate the association between MTHFR C677T polymorphism and the risk of DM or DN. Overall, our meta-analysis for DN versus healthy controls produced significant results for all genetic contrasts except for the co-dominant model (allele contrast: OR = 2.24, 95%CI: 1.88–2.65, p < 0.00001, Pheterogeneity = 0.49). However, the meta-analysis for DM versus healthy controls produced non-significant results for all contrasts (allele contrast: OR = 1.12, 95%CI: 0.92–1.35, p = 0.25, Pheterogeneity = 0.07). In addition, the meta-analysis for DM versus DN produced significant results for all contrasts (allele contrast: OR = 1.88, 95%CI: 1.65–2.15, p < 0.00001, Pheterogeneity = 0.83). The current meta-analysis suggested that MTHFR C677T polymorphism might influence DN risk, but not for DM in the Chinese population.
Anterior pituitary type II thyroxine 5'-deiodinase activity is not affected by lesions of the hypothalamic paraventricular nucleus which profoundly depress pituitary thyrotropin secretion.

Bilateral destruction of the hypothalamic paraventricular nuclei (PVN) produced a profound depression of plasma TSH and the median eminence TRH concentration in hypothyroid rats. Anterior pituitary type II iodothyronine 5'-deiodinase (5'-D) activity was consistently lower but not significantly different in sham- and PVN-lesioned rats. Treatment with suboptimal replacement doses of 0.15 and 0.75 micrograms T4/100 g BW.day produced a graded depression of plasma TSH in the PVN (P less than 0.02), but not in the sham (P greater than 0.8) groups. Adenohypophyseal 5'-D was depressed in both sham and PVN groups by the highest T4 dose. Plasma T4 was much lower in PVN than in sham rats given comparable doses of T4 (P less than 0.001), but plasma T3 was not significantly different. This suggests that an increase in peripheral T4 metabolism was produced by PVN lesions. Our data indicate that changes in adenohypophyseal 5'-D activity are not responsible for the decrease in plasma TSH in PVN-lesioned rats and that neither the PVN nor endogenous TRH plays a significant role in the regulation of anterior pituitary 5'-D activity.
Multilevel Modelling with Spatial Interaction Effects with Application to an Emerging Land Market in Beijing, China

This paper develops a methodology for extending multilevel modelling to incorporate spatial interaction effects. The motivation is that classic multilevel models are not specifically spatial. Lower level units may be nested into higher level ones based on a geographical hierarchy (or a membership structure—for example, census zones into regions) but the actual locations of the units and the distances between them are not directly considered: what matters is the groupings but not how close together any two units are within those groupings. As a consequence, spatial interaction effects are neither modelled nor measured, confounding group effects (understood as some sort of contextual effect that acts ‘top down’ upon members of a group) with proximity effects (some sort of joint dependency that emerges between neighbours). To deal with this, we incorporate spatial simultaneous autoregressive processes into both the outcome variable and the higher level residuals. To assess the performance of the proposed method and the classic multilevel model, a series of Monte Carlo simulations are conducted. The results show that the proposed method performs well in retrieving the true model parameters whereas the classic multilevel model provides biased and inefficient parameter estimation in the presence of spatial interactions. An important implication of the study is to be cautious of an apparent neighbourhood effect in terms of both its magnitude and statistical significance if spatial interaction effects at a lower level are suspected. Applying the new approach to a two-level land price data set for Beijing, China, we find significant spatial interactions at both the land parcel and district levels.
What Students Say Versus What They Do Regarding Scientific Inquiry

We teach a course for elementary education undergraduates that gives students an opportunity to conduct open-ended scientific inquiry and pursue their own scientific questions in much the same way that practicing research scientists do. In this study, we compared what our students say declaratively about the nature of science (NOS) in surveys and interviews with what they do procedurally when engaged in authentic scientific practice. Initially, we were surprised when our students showed very little change on two different validated NOS questionnaires, adhering to seemingly memorized definitions of key NOS vocabulary such as “science” and “experiment. ” In contrast, on procedural measures of NOS understanding, students developed a decidedly sophisticated approach to answering scientific questions. Our data suggest that students’ declarative understandings about the NOS are not a reliable measure of students’ ability to engage productively in scientific practices and vice versa. We discuss why this might be and consider the implications of this disconnect on identifying the best approach to NOS instruction and on future science education research.
The temperature dependence and involvement of mitochondria permeability transition and caspase activation in damage to organotypic hippocampal slices following in vitro ischemia

The aggravating effect of hyperglycemia on ischemic brain injury can be mimicked in a model of in vitro ischemia (IVI) using murine hippocampal slice cultures. Using this model, we found that the damage in the CA1 region following IVI in the absence or presence of 40 mm glucose (hyperglycemia) is highly temperature dependent. Decreasing the temperature from 35 to 31°C during IVI prevented cell death, whereas increasing the temperature by 2°C markedly aggravated damage. As blockade of the mitochondrial permeability transition (MPT) is equally effective as hypothermia in preventing ischemic cell death in vivo, we investigated whether inhibition of MPT or of caspases was protective following IVI. In the absence of glucose, the MPT blockers cyclosporin A and MeIle4‐CsA but not the immunosuppressive compound FK506 diminished cell death. In contrast, following hyperglycemic IVI, MPT blockade was ineffective. Also, the pan‐caspase inhibitor Boc‐Asp(OMe)fluoromethyl ketone did not decrease cell death in the CA1 region following IVI or hyperglycemic IVI. We conclude that cell death in the CA1 region of organotypic murine hippocampal slices following IVI is highly temperature dependent and involves MPT. In contrast, cell death following hyperglycemic IVI, although completely prevented by hypothermia, is not mediated by mechanisms that involve MPT or caspase activation.