Miracle-dz/UniFAR-FTUs huggingface dataset:
Rapid and Specific Enrichment of Culturable Gram Negative Bacteria Using Non-Lethal Copper-Free Click Chemistry Coupled with Magnetic Beads Separation

Currently, identification of pathogenic bacteria present at very low concentration requires a preliminary culture-based enrichment step. Many research efforts focus on the possibility to shorten this pre-enrichment step which is needed to reach the minimal number of cells that allows efficient identification. Rapid microbiological controls are a real public health issue and are required in food processing, water quality assessment or clinical pathology. Thus, the development of new methods for faster detection and isolation of pathogenic culturable bacteria is necessary. Here we describe a specific enrichment technique for culturable Gram negative bacteria, based on non-lethal click chemistry and the use of magnetic beads that allows fast detection and isolation. The assimilation and incorporation of an analog of Kdo, an essential component of lipopolysaccharides, possessing a bio-orthogonal azido function (Kdo-N3), allow functionalization of almost all Gram negative bacteria at the membrane level. Detection can be realized through strain-promoted azide-cyclooctyne cycloaddition, an example of click chemistry, which interestingly does not affect bacterial growth. Using E. coli as an example of Gram negative bacterium, we demonstrate the excellent specificity of the technique to detect culturable E. coli among bacterial mixtures also containing either dead E. coli, or live B. subtilis (as a model of microorganism not containing Kdo). Finally, in order to specifically isolate and concentrate culturable E. coli cells, we performed separation using magnetic beads in combination with click chemistry. This work highlights the efficiency of our technique to rapidly enrich and concentrate culturable Gram negative bacteria among other microorganisms that do not possess Kdo within their cell envelope.
Automatic construction of domain and aspect specific sentiment lexicons for customer review mining

Automatically analyzing the opinions expressed in customer reviews is of high relevance in many application scenarios, e.g., market research, trend analysis, or reputation management. A great share of current sentiment analysis approaches makes use of special purpose lexicons that provide information about the polarity (e.g., positive or negative) of individual words and phrases. One major challenge is that the actual sentiment polarity of a specific expression is often context dependent (e.g., "long+ battery life" vs. "long- flash recycle time"). However, the vast majority of existing approaches focuses on creating general purpose lexicons. Especially in the context of mining customer review data, the use of such lexicons is rather suboptimal as they fail to adequately reflect the domain specific lexical usage. We propose a novel method that allows to automatically adapt and extend existing lexicons to a specific product domain. We follow a corpus-based approach and exploit the fact that many customer reviews exhibit some form of semi-structure. The method is fully automatic and thus scales well across different product domains. Our experiments show that the extracted lexicons are highly accurate and significantly improve the performance in a sentiment classification scenario.
INFLUENCE OF PERCEIVED USEFULNESS AND EASE OF USE ON STUDENTS’ CONTINUOUS INTENTION IN LEARNING ON-LINE ENGLISH LESSONS: AN EXTENDED TAM

There are huge numbers of English lessons available on-line and are ready to be used by TEFL and ELT teachers for teaching Listening, Speaking, Reading, Writing, Pronunciation, Grammar, etc. Some on-line English lessons may receive less attention from the students as the web pages are too complicated or the addresses are too difficult to be accessed. It can be caused by improper choice of learning template such as Moodle, Edmodo, the Blackboard system, etc or caused by type of application used such as Wondershare, PPT Macros, Aurora 3D, Zondle, Hotpotato, iMindmap, Drop Box, Edmodo, ToolBook, Smashwords, Amazon Kindle DX, PowToon, and so forth. Therefore, it is critical to understand students’ continuous intention in following on-line English lessons. This study was aimed at assessing the influence of Perceived Usefulness and Ease of Use on Students’ Continuous Intention in learning on-line English Lessons. There were 230 pre-service English students (4A-4E classes) who took compulsory course of Teaching Media/ Instructional Technology during the academic year of 2016/2017.The students were divided into several groups for joining on-line English lessons of Listening, Speaking, Reading, Writing, Pronunciation, Grammar, Vocabulary, and TOEFL. While joining those on-line lessons, the researcher measured their continuous attention from ease-of-use, and perceived usefulness of extended Technology Acceptance Model (TAM). The data were collected through questioners and analysed by using Structural Equation Modelling (SEM). The results indicate that the students’ intentions are influenced positively by the design of the lessons, lessons delivery/ease-of-use, and their perceived usefulness. Perceived usefulness (PU) influenced more on students’ motivation than ease-of-use (PEOU). It is recommended that designers of on-line English lessons should consider students’ prior knowledge of ICT, pedagogical principles, and technological barrier in the students’ location for receiving more positive enduring intention.
Hip Microfracture

Microfracture is a marrow-stimulating technique used in the hip to treat cartilage defects associated with femoro-acetabular impingement, instability, or traumatic hip injury. These defects have a low probability of healing spontaneously and therefore often require surgical intervention. Originally adapted from the knee, microfracture is part of a spectrum of cartilage repair options that include palliative procedures such as debridement and lavage, reparative procedures such as marrow-stimulating techniques (abrasion arthroplasty and microfracture), and restorative procedures such as autologous chondrocyte implantation and osteochondral allograft/autografts. The basic indications for microfracture of the hip include focal and contained lesions typically less than 4 cm in diameter, full-thickness (Outerbridge grade IV) defects in weightbearing areas, unstable lesions with intact subchondral bone, and focal lesions without evidence of surrounding chondromalacia. Although not extensively studied in the hip, there are some small clinical series with promising early outcomes. Although the widespread use of microfracture in the hip is hindered by difficulties in identifying lesions on preoperative imaging and instrumentation to circumvent the femoral head, this technique continues to gain acceptance as an initial treatment for small, focal cartilage defects.
Combined road prediction and target tracking in collision avoidance

Detection and tracking of other vehicles and lane geometry will be required for many future intelligent driver assistance systems. By integrating the estimation of these two features into a single filter, a more optimal utilization of the available information can be achieved. For example, it is possible to improve the lane curvature estimate during bad visibility by studying the motion of other vehicles. This paper derives and evaluates various approximations that are needed in order to deal with the non-linearities that are introduced by such an approach.
Exploring Co-presence Enabled Collaboration in Social Computing Inspired Enterprise Systems

This research describes the findings from an interpretive case study that explores the interplay between social computing (SC) and enterprise systems (ES). A fundamental shift is evident in how organisations become more effective through the adoption of SC capabilities. As process centric ES continues to pose challenges, an SC inspired, people-centric ES has become a medium for efficient interaction and collaboration across the divisions of an organisation. In this organisational reality, we explore the role of virtual co-presence of users on collaboration in ES. Our findings indicate that virtual co-presence enabled interactions, when focused and sustained over time, could facilitate collaboration for sharing of knowledge. An understanding of how users interact in mediated encounters contributes to our knowledge of how focused interactions may enable collaborations in ES. By drawing on the findings, the research seeks to outline some implications for the practice of a collaborative ES for the contemporary organisations.
A 9-bit Quadrature Direct Digital Synthesizer Implemented in 0.18-$\mu{\hbox {m}}$ SiGe BiCMOS Technology

This paper describes a 9-bit 6.2-GHz low power quadrature direct digital synthesizer (DDS) implemented in a 0.18-mum SiGe BiCMOS technology. With a 9-bit pipeline accumulator and two 8-bit sine-weighted current steering DACs, this DDS is capable of generating quadrature sinusoidal waveforms up to 3.15 GHz with a maximum clock frequency of 6.2 GHz. Packed with more than 13 500 transistors, the quadrature DDS occupies an active area of 2.3 times 2.5 mm2 and a total die area of 3.0 times 3.0 mm2. The measured spurious-free dynamic range is approximately 26 dBc at a clock frequency 6.2 GHz. At the maximum clock frequency, the power consumption of the DDS is 2.5 W with 3.3- and 4.0-V power supplies for the digital and analog parts, respectively. The DDS thus achieves a power efficiency figure-of-merit of 5.04 GHz/W/phase. The DDS chips were packaged with 48-pin ceramic leadless chip carriers and air cooling was used during the measurement.
Needs assessment of youths in residential child care in Portugal / Evaluación de necesidades de jóvenes en acogimiento residencial en Portugal / Evaluación de necesidades de jóvenes en acogimiento residencial en Portugal

Abstract Residential child care in Portugal is the most common protection measure in cases of family separation, since family foster care is still very rare. However, little is known about the needs of these children and young people. The purpose of this study is to describe the profile of adolescents close to legal adulthood who are in residential care, comparing it with a Spanish sample. The sample is composed of 345 young people aged 16 to 17 on whom information has been collected regarding their history of protection, family background, schooling, mental health and offending behaviour. A Spanish sample was selected as a comparison group (N = 384). The results reveal the complexity and special seriousness of the profile of young people in residential care in Portugal compared to a Spanish sample, highlighting a high presence of mental health problems and especially suicidal behaviours. Given these results, efforts should be focused on developing specialized residential care programmes adapted to the specific needs of these young people.
Task complexity in history textbooks: A multidisciplinary case study on triangulation in history education research

The purpose of history education in Austria has changed over at least the last decade. While the focus used to be to give students a master narrative of the national past based on positivist knowledge, the current objective of history education is to foster historical thinking processes that enable students to form transferable skills in the self-reflected handling and creation of history. A key factor in fostering historical thinking is the appropriation of learning tasks. This case study measures the complexity of learning tasks in Austrian history textbooks as one important aspect of their quality. It makes use of three different approaches to complexity to triangulate the notion: general task complexity (GTC), general linguistic complexity (GLC), and domain-specific task complexity (DTC). The question is which findings can be offered by the specific strengths and limitations of the different methodological approaches to give new insights into the study of task complexity in the domain of history education research. By pursuing multidisciplinary approaches in a triangulating way, the case study opens up new prospects for this field. Besides offering new insights on measuring the complexity of learning tasks, the study illustrates the need for further research in this field – not only related to the development of analytical frameworks, but also regarding the notion of complexity in the context of historical learning itself.
Neuronal Acetylcholine Receptors with α7 Subunits Are Concentrated on Somatic Spines for Synaptic Signaling in Embryonic Chick Ciliary Ganglia

Nicotinic acetylcholine receptors containing α7 subunits are widely distributed in the vertebrate nervous system. In the chick ciliary ganglion such receptors generate large synaptic currents but appear to be excluded from postsynaptic densities on the cells. We show here that α7-containing receptors are concentrated on somatic spines in close proximity to putative sites of presynaptic transmitter release. Intermediate voltage electron microscopy on thick sections, together with tomographic reconstruction, permitted three-dimensional analysis of finger-like projections emanating from cell bodies. The projections were identified as spines based on their morphology, cytoskeletal content, and proximity to presynaptic elements. Bothin situ and after ganglionic dissociation, the spines were grouped on the cell surface and tightly folded into mats. Immunogold labeling of receptors containing α7 subunits showed them to be preferentially concentrated on the somatic spines. Postsynaptic densities were present in vivo both on the soma near spines and occasionally on the spines themselves. Synaptic vesicle-filled projections from the presynaptic calyx were interdigitated among the spines. Moreover, the synaptic vesicles often abutted the membrane and sometimes included Ω profiles as if caught in an exocytotic event, even when no postsynaptic densities were juxtaposed on the spine. The results suggest several mechanisms for delivering transmitter to α7-containing receptors, and they support new ideas about synaptic signaling via spines. They also indicate that neurons must have specific mechanisms for targeting α7-containing receptors to desired locations.
THE ASSEMBLY OF MATTER IN GALAXY CLUSTERS

We study the merging history of dark matter haloes that end up in rich clusters, using N-body simulations of a scale-free universe. We compare the predictions of the extended Press &38; Schechter (P&38;S) formalism with several conditional statistics of the protocluster matter: the mass distribution and relative abundance of progenitor haloes at different redshifts, the infall rate of progenitors within the protocluster, the formation redshift of the most massive cluster progenitor, and the accretion rates of other haloes on to it. The high quality of our simulations allows an unprecedented resolution in the mass range of the studied distributions. We also present the global mass function for the same cosmological model. We find that the P&38;S formalism and its extensions cannot simultaneously describe the global evolution of clustering and its evolution in a protocluster environment. The best-fitting P&38;S model for the global mass function is a poor fit to the statistics of cluster progenitors. This discrepancy is in the sense of underpredicting the number of high-mass progenitors at high redshift. Although the P&38;S formalism can provide a good qualitative description of the global evolution of hierarchical clustering, particular attention is needed when applying the theory to the mass distribution of progenitor objects at high redshift.
Provider-Initiated HIV testing and counseling among patients with presumptive tuberculosis in Democratic Republic of Congo

Introduction Provider-initiated HIV testing and counseling (PITC) of patients with presumptive tuberculosis (TB) is not widely implemented and the burden of HIV among them is not well characterized. We assessed the uptake of PITC and prevalence of HIV among patients with presumptive TB in primary care settings in the Democratic Republic of Congo. Methods PITC was implemented in primary care TB clinics in Kinshasa and Kisangani, respectively. In each of the clinics, all patients presenting with cough lasting more than two weeks or any other symptom suggestive of TB were offered HIV testing and counseling and those found to be HIV+ were linked to HIV care and treatment. Results Between November 2011 and June 2013, 43,145 patients with presumptive TB were registered in 65 clinics in Kinshasa of whom 84.0% were counseled; 92.4% of those counseled were tested and 4,320 (12.9%) were found to be HIV+. Similarly, in Kisangani, of the 6,687 patients with presumptive TB were registered in 13 clinics, 80.5% were counseled; 99.3% were tested for HIV and 619 (11.6%) were found to be HIV+. Conclusion Implementation of PITC among patients with presumptive TB in primary care clinics was associated with high uptake of HIV testing and identification of high number of HIV+ patients.
The Uncertain Relationship between Board Composition and Firm Performance

We survey the evidence on the relationship between board composition and firm performance. Boards of directors of American public companies that have a majority of independent directors behave differently, in a number of ways, than boards without such a majority. Some of these differences appear to increase firm value; others may decrease firm value. Overall, within the range of board compositions present today in large public companies, there is no convincing evidence that greater board independence correlates with greater firm profitability or faster growth. In particular, there is no empirical support for current proposals that firms should have "supermajority-independent boards" with only one or two inside directors. To the contrary, there is some evidence that firms with supermajority-independent boards are less profitable than other firms. This suggests that it may be useful for firms to have a moderate number of inside directors (say three to five on an average-sized eleven member board). We offer some possible explanations for these results, based on board dynamics, the informational advantages possessed by inside (and, often, affiliated) directors, and the value of interaction between different types of directors who bring different strengths to the board.
Innovations in Performance Measurement: Trends and Research Implications

The objective of this paper is to foster research on recent innovations in performance measurement by providing a rich description of emerging measurement practices and suggesting directions for future research. Using survey data collected by consulting firms and government organizations, we examine three measurement trends: (1) economic value measures, (2) non-financial performance measures and the balanced scorecard, and (3) performance measurement initiatives in government agencies. Existing research on these topics is reviewed and research opportunities are highlighted.
Relation between grit, competitive levels, and athletic events in Japanese athletes

This study examined the relation between grit, competitive levels, and athletic events in Japanese athletes (N = 496, 428 men; mean age = 20.6 years, SD = 0.6). The results indicated that consistency of interest was marginally positively associated with competitive levels in closed-skills sports, but a negative association existed between the two in open-skills sports. In contrast, the interaction of athletic events and perseverance of effort were not significantly associated with competitive levels. These findings suggest that the combination of grit and participation in athletic events has a different relation to competitive levels.
Christian University Students’ Attitudes to Gender: Constructing Everyday Theologies in a Post-Feminist Climate

This article explores how religion shapes approaches to gender amongst university students in the United Kingdom, focusing on how attitudes about gender interact with their Christian identities. Drawing from 68 semi-structured interviews conducted at five universities, the article identifies three main approaches Christian students adopt when asked how faith affects their views on gender: the individualized approach, the egalitarian approach and the conservative approach. The article outlines the permutations of these approaches, showing their points of similarity and difference, and argues that feminism, biological essentialism and notions of reasonableness or “cultural common sense” feature in all three, being integral to the gender discourse of “post-feminist” UK society. The article argues that religion functions as a resource in Christian students’ gender attitudes, alongside other resources such as friends or family, and is deployed to justify both egalitarianism and gender conservatism. Christian students are constructing “everyday theologies” that integrate religious resources with other social resources, generating divergent egalitarian and conservative interpretations, mirroring patterns in “post-feminist” UK society more generally.
ORWELL´S ANIMAL FARM AND THE TRANSLATION CHALLENGES (ENGLISH-PORTUGUESE) REGARDING ANIMAL PERSONIFICATION

In this study, we conducted a comparative and contrastive analysis of the personification of the animals and their translation from English to Portuguese language. Animal personification is a likely source of cross-cultural misunderstanding, so that if translators do not contribute to remove this misinterpretation, communication efficiency will be jeopardized. We assumed that in translation of animal personification, where the personifications are different or opposite in the two languages involved in the translation, the translators should guide their efforts toward adopting the appropriate animal terms in the translation. In this study, the situation of translation was more complicated as the source text (Orwell´s Animal Farm) represents itself the image of some animals which are different from or contrary to the English readers´ expectations. It was assumed that representing the animals in the source text with different or opposite image to the expectations of the source text readers was the author´s intention; hence, this style should be also observed by the translators. Therefore, regarding the relationship between the source language and the target language in terms of the type of similarities and differences between the personification of the animals and the intention of the author, different cases of translation were pointed out and discussed. Afterwards, possible translation methods were proposed for each condition. In fact, we aimed to determine whether these methodologies are applicable to a work like Animal Farm. However, in translating Animal Farm, all the Portuguese translators kept all the same animal terms in the target text, and it seems that the readers do not prejudice with this. Apparently, the translators were not required to explain everything to the readers, as Orwell did not do. In fact, the author and the translators made use of the reader´s willing suspension of disbelief which helps them in temporarily accepting some unusual ideas and being absorbed in the text and so being entertained by this artistic creativity.
The SPHINX-II speech recognition system: an overview

In order for speech recognizers to deal with increased task perplexity, speaker variation, and environment variation, improved speech recognition is critical. Steady progress has been made along these three dimensions at Carnegie Mellon. In this paper, we review the SPHINX-II speech recognition system and summarize our recent efforts on improved speech recognition.