Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional magnetic resonance imaging of auditory cortex: with special reference to the side of aural stimulation.

PURPOSE To determine whether left- or right-side uniaural stimulation produces different fMRI activation patterns. METHODS Subjects were 12 volunteers (8 right-handed, 4 left-handed) with normal hearing. Functional imaging using FE-type multishot echo planar imaging was obtained in the axial plane during pure-tone and pseudoword tasks. Auditory stimuli were presented to each ear individually. In pure-tone tasks, subjects heard clustered sequences at 2000 Hz. In pseudoword tasks, subjects heard spoken Japanese syllables. The numbers of activated pixels in the auditory cortex were counted and compared for pure-tone and pseudoword tasks, as was activation according to the side of aural stimulation. RESULTS In right-handed subjects, prominent activation in pure-tone tasks was noted in the dominant hemisphere in 100% of cases and was unrelated to the side of the stimulation. In pseudoword tasks, prominent activation was noted on the side contralateral to the stimulus in 62.5-100% of cases. In left-handed subjects, prominent activation was noted on the side contralateral to the stimulus in both pure-tone and pseudoword tasks. CONCLUSION Left- and right-side stimulation produced differences in fMRI responses, especially between pure-tone and pseudoword tasks. Moreover, right-handedness and left-handedness affected results. This type of auditory fMRI may be a noninvasive indicator of language lateralization.
Perception of Affective Body Movements in HRI across Age Groups: Comparison between Results from Denmark and Japan

Social robots are envisioned to move into unrestricted environments where they will be interacting with naive users (in terms of their experience as robot operators). Thus, these robots are also envisioned to exploit interaction channels that are natural to humans like speech, gestures, or body movements. A specificity of these interaction channels is that humans do not only convey task-related information but also more subtle information like e.g. Emotions or personal stance through these channels. Thus, to be successful and not accidentally jeopardizing an interaction, robots need to be able to understand these implicit connotations of the signals (often called social signal processing) in order to generate appropriate signals in a given interaction context. One main application area that is envisioned for social robots is related to elder care, but little is known on how seniors will perceive robots and the signals they produce. In this paper we focus on affective connotations of body movements and investigate how the perception of body movements of robots is related to age. Inspired by a study from Japan, we introduce culture as a variable in the experiment and discuss the difficulties of cross-cultural comparisons. The results show that there are certain age-related differences in the perception of affective body movements, but not as strong as in the original study. A follow up experiment puts the affective body movements into context and shows that recognition rates deteriorate for older participants.
Attitudes Towards Foreign-born Settlers: Finland in a Comparative Perspective

The present paper focuses on the Finnish attitudinal climate towards foreign-born settlers, i.e. immigrants, refugees and asylum seekers in a comparative perspective. Two theoretical approaches are tested: 1) the socio-economic explanation in which prejudice is seen as an outcome of the original majority population fearing a decline of their socio-economic position, and 2) a culturally oriented explanation which refers to fears concerning the possible negative cultural effects of immigration. Using survey data from the ? rst round of the European Social Survey (ESS) on Finland and 18 other countries, we ? nd that at the most general level, Finns do hold comparatively negative attitudes towards increasing the number of immigrants in Finland. However, as we turn to more speci? c items on economic and especially cultural threat, we ? nd that Finns are as tolerant or even more tolerant than other Europeans. Multivariate analyses show that both socio-economic factors and values, ideologies and religiosity may generate prejudice. The two theoretical approaches should, thus, not be taken as alternatives but rather as complementary theories.
Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9

Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp. , the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use the RNA-guided Cas9 nuclease to efficiently generate knockouts without selection, and to introduce point mutations and epitope tags into the T. gondii genome. These methods will streamline the functional analysis of parasite genes and enable high-throughput engineering of their genomes.
Serum 25-hydroxy vitamin D levels in patients with acne vulgaris and its association with disease severity.

BACKGROUND Since vitamin D is a recent known immunoregulatory factor in some diseases which are addressed in immune system disorders such as SLE, [psoriasis] and others. OBJECTIVE To determine the serum levels of 25-hydroxy vitamin D3 [25(OH)D] in patients with acne vulgaris and its association with clinical features. METHODS This cross-sectional study was conducted over months. This study included 39 patients with acne vulgaris and 40 healthy controls. Subjects who did not use alcohol, vitamin D supplements, oral steroids or PUVA and/or NBUVB for more than three months were included. Serum 25(OH)D levels were measured. Baseline demographics, family history and comorbidities like PCO were recorded. Statistical analyses were performed using SPSS 16.0. 0. RESULTS The median concentration of 25(OH)D was 8.4 ng/mL (range: 1.4-99) in patients and 10.4 ng/mL (range: 3.1-56.7) in controls, with no statistical significant difference. PCOS was a significant predictor of the occurrence of acne vulgaris (OR=6.25; 95% CI: 1.52-25.66; p=0.01). There were no significant associations between severity of disease and serum 25(OH)D levels (rs=-0.12, p=0.45), age (rs=-0.28, p=0.09), BMI (rs=-0.12, p=0.46), age at onset of disease (rs=-0.08, p=0.63) and duration of disease (rs=-0.10, p=0.54). CONCLUSION Based on the previous studies this is highly suspected that vitamin D would be a prominent factor in acne patients and more performances with bigger sample size could be useful to get positive results.
Attachment behavior and health: relationships at work and home

This article examines the relationships between interpersonal attachment orientations, health, and social support. Four hypotheses specified relationships between three interpersonal attachment orientations (interdependent, counterdependent, and overdependent), physical and psychological symptoms, and social support. Data were collected from 297 students, the majority of whom were also full-time employees. An interdependent orientation was negatively related to social dysfunction. Counterdependent and overdependent orientations were negatively related to health. A counterdependent orientation was negatively related to social support, while an overdependent orientation was positively related to social support from co-workers and family. Copyright © 1999 John Wiley & Sons, Ltd.
An Analysis Of The Reasons Offered By Post-Graduate Diploma In Education Students In Botswana For Opting For A Teaching Career

The purpose of this paper was to investigate and analyze the reasons advanced by Post Grade Diploma in Education students (PGDE) for choosing teaching as a career. The subjects of the study were 102 students of which 58 were females and 44 were males. The average age for this group of students was 23. Data were collected using a questionnaire in which a quantitative approach was employed using closed and open-ended questions. The findings of the study revealed that, PGDE students choose the teaching profession mainly for extrinsic reasons such as job security and economic advancement. The paper also indicated that student teachers chose teaching as a career because they wanted to serve the community.
Human Genome Replication Proceeds through Four Chromatin States

Advances in genomic studies have led to significant progress in understanding the epigenetically controlled interplay between chromatin structure and nuclear functions. Epigenetic modifications were shown to play a key role in transcription regulation and genome activity during development and differentiation or in response to the environment. Paradoxically, the molecular mechanisms that regulate the initiation and the maintenance of the spatio-temporal replication program in higher eukaryotes, and in particular their links to epigenetic modifications, still remain elusive. By integrative analysis of the genome-wide distributions of thirteen epigenetic marks in the human cell line K562, at the 100 kb resolution of corresponding mean replication timing (MRT) data, we identify four major groups of chromatin marks with shared features. These states have different MRT, namely from early to late replicating, replication proceeds though a transcriptionally active euchromatin state (C1), a repressive type of chromatin (C2) associated with polycomb complexes, a silent state (C3) not enriched in any available marks, and a gene poor HP1-associated heterochromatin state (C4). When mapping these chromatin states inside the megabase-sized U-domains (U-shaped MRT profile) covering about 50% of the human genome, we reveal that the associated replication fork polarity gradient corresponds to a directional path across the four chromatin states, from C1 at U-domains borders followed by C2, C3 and C4 at centers. Analysis of the other genome half is consistent with early and late replication loci occurring in separate compartments, the former correspond to gene-rich, high-GC domains of intermingled chromatin states C1 and C2, whereas the latter correspond to gene-poor, low-GC domains of alternating chromatin states C3 and C4 or long C4 domains. This new segmentation sheds a new light on the epigenetic regulation of the spatio-temporal replication program in human and provides a framework for further studies in different cell types, in both health and disease.
Examination of phonological working memory in specifically language-impaired children

Abstract The present study examined the phonological memory capacity, rate of articulation, phonological-encoding, and perceptual-processing abilities of 13 well-defined, specifically language-impaired (SLI) children and 13 younger, language-matched normal (NL) children. The results of a nonsense word repetition task showed that SLI children repeated significantly fewer multisyllabic nonsense words than their NL peers. However, SLI and NL children were found to have comparable articulation rates, even when producing the longest nonsense word stimuli. Both SLI and NL children showed sensitivity to the phonological similarity effect, indicating that SLI children had intact phonological-encoding abilities. The results of a nonsense word discrimination task revealed that SLI children had greater difficulty perceptually processing 4-syllable nonsense words. Taken together, these findings were interpreted to be consistent with Gathercole and Baddeley's (1990) claim that SLI children have reduced phonological storage capacity. However, the capacity deficit account may require revision to include the possibility that the phonological storage deficit of some SLI children may have a perceptual basis (i.e., difficulty with processes related to item identification).
A prospective assessment of the role of bronchoscopy and bronchoalveolar lavage in evaluation of children with pulmonary tuberculosis.

OBJECTIVE To assess the diagnostic yield of fibreoptic bronchoscopy and bronchoalveolar lavage (BAL) compared to gastric aspiration (GA) in children with probable pulmonary tuberculosis (TB). METHODS Children with probable pulmonary TB were subjected to bronchoscopy, BAL and gastric aspiration. The samples were examined for acid fast bacilli (AFB) on smear. RESULTS A total of 52 children underwent bronchoscopy with BAL and GA. AFB on smear was identified in 19 (36.5%); BAL was positive in 16 (30.8%) and GA was positive in 11 (21.15%). GA alone was positive in 3 (5.67%) while BAL alone was positive in 8 (15.38%). Bronchoscopy detected airways' abnormality in 29 (56%) of patients. One GA and BAL detected AFB in majority of patients. CONCLUSION The diagnostic yield for AFB from BAL was better than that from GA in children with probable pulmonary TB. Combining one GA and bronchoscopy may improve AFB yield in children with pulmonary TB.
Women use voice parameters to assess men's characteristics

The purpose of this study was: (i) to provide additional evidence regarding the existence of human voice parameters, which could be reliable indicators of a speaker's physical characteristics and (ii) to examine the ability of listeners to judge voice pleasantness and a speaker's characteristics from speech samples. We recorded 26 men enunciating five vowels. Voices were played to 102 female judges who were asked to assess vocal attractiveness and speakers' age, height and weight. Statistical analyses were used to determine: (i) which physical component predicted which vocal component and (ii) which vocal component predicted which judgment. We found that men with low-frequency formants and small formant dispersion tended to be older, taller and tended to have a high level of testosterone. Female listeners were consistent in their pleasantness judgment and in their height, weight and age estimates. Pleasantness judgments were based mainly on intonation. Female listeners were able to correctly estimate age by using formant components. They were able to estimate weight but we could not explain which acoustic parameters they used. However, female listeners were not able to estimate height, possibly because they used intonation incorrectly. Our study confirms that in all mammal species examined thus far, including humans, formant components can provide a relatively accurate indication of a vocalizing individual's characteristics. Human listeners have the necessary information at their disposal; however, they do not necessarily use it.
Enhancement of Adeno-Associated Virus Infection by Mobilizing Capsids into and Out of the Nucleolus

ABSTRACT Adeno-associated virus (AAV) serotypes are being tailored for numerous therapeutic applications, but the parameters governing the subcellular fate of even the most highly characterized serotype, AAV2, remain unclear. To understand how cellular conditions control capsid trafficking, we have tracked the subcellular fate of recombinant AAV2 (rAAV2) vectors using confocal immunofluorescence, three-dimensional infection analysis, and subcellular fractionation. Here we report that a population of rAAV2 virions enters the nucleus and accumulates in the nucleolus after infection, whereas empty capsids are excluded from nuclear entry. Remarkably, after subcellular fractionation, virions accumulating in nucleoli were found to retain infectivity in secondary infections. Proteasome inhibitors known to enhance transduction were found to potentiate nucleolar accumulation. In contrast, hydroxyurea, which also increases transduction, mobilized virions into the nucleoplasm, suggesting that two separate pathways influence vector delivery in the nucleus. Using a small interfering RNA (siRNA) approach, we then evaluated whether nucleolar proteins B23/nucleophosmin and nucleolin, previously shown to interact with AAV2 capsids, affect trafficking and transduction efficiency. Similar to effects observed with proteasome inhibition, siRNA-mediated knockdown of nucleophosmin potentiated nucleolar accumulation and increased transduction 5- to 15-fold. Parallel to effects from hydroxyurea, knockdown of nucleolin mobilized capsids to the nucleoplasm and increased transduction 10- to 30-fold. Moreover, affecting both pathways simultaneously using drug and siRNA combinations was synergistic and increased transduction over 50-fold. Taken together, these results support the hypothesis that rAAV2 virions enter the nucleus intact and can be sequestered in the nucleolus in stable form. Mobilization from the nucleolus to nucleoplasmic sites likely permits uncoating and subsequent gene expression or genome degradation. In summary, with these studies we have refined our understanding of AAV2 trafficking dynamics and have identified cellular parameters that mobilize virions in the nucleus and significantly influence AAV infection.
Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina

Purpose The CERKL gene encodes for ceramide kinase-like, a novel protein of unknown function. CERKL mutations are associated with a severe retinal phenotype. The purpose of this work was to investigate alternative splicing, and the temporal and spatial expression pattern of CERKL in the mouse retina. Methods Reverse Transcription-Polymerase Chain Reaction (RT–PCR) analysis of mouse retina RNA was used to study the expression of Cerkl at various developmental time points, and to identify its various splice-isoforms. A specific anti-CERKL antibody was developed and used for immunostaining to study the localization of the endogenous CERKL protein in retina-derived cell lines and in the mouse retina. Results Cerkl is expressed in the mouse eye as early as embryonic day 14. A total of seven different Cerkl splice-isoforms were identified in the mouse retina. The subcellular localization of CERKL in retina-derived cell lines is variable: CERKL is diffusely distributed in the cytoplasm, and in many cells, it is highly concentrated in the perinuclear region. In most, but not all cells, CERKL is also highly concentrated in the nucleus. In the mouse retina, CERKL is located in the ganglion cell layer, in amacrine cells of the inner nuclear layer, and in photoreceptors. CERKL is highly expressed in cone photoreceptors; however, its expression level in rod photoreceptors is very low. In cultured cells, CERKL is detected in the nucleus, but in retinal cells in situ, it is mostly located in the cytoplasm. Conclusions The expression of Cerkl in both mature and embryonic mouse retina and the severe retinal phenotype associated with human CERKL mutations indicate that this gene plays a crucial role in retinal activity, and that it may be important for retinal development as well. The high expression level of CERKL in cones correlates with the CERKL-associated phenotype in humans. Whether nucleocytoplasmic transport of CERKL actually occurs in vivo under certain conditions and its functional significance remain to be discovered.
Effect of GA-sensitivity on wheat early vigor and yield components under deep sowing

Establishment of seedlings is a key factor in achievement of uniform field stands and, consequently, stable yields. Under Mediterranean conditions, soil moisture in the upper layer is limited and seedlings may be exposed to frequent dehydration events. The presence of the Reduced height (Rht)-B1b and Rht-D1b semi-dominant dwarfing alleles results in insensitivity to gibberellin (GAI) and, hence, poor emergence from deep sowing. Introduction of alternative dwarfing genes and, thereby, preservation of the gibberellin response (GAR) and coleoptile length, contributes to better emergence from deep sowing. Initially 47 wheat cultivars carrying different Rht alleles were screened for their ability to emerge from deep sowing, and then 17 of them were selected for detailed physiological characterization in the field. The modern wheat lines containing GAI alleles showed significantly lower percentages of emergence from deep sowing than the GAR lines, i.e., 52 and 74%, respectively. Differences in early developmental stages were associated with grain yield, as indicated by a reduction of 37.3% in the modern GAI cultivars. Our results demonstrate the potential of alternative dwarfing genes for improving seedling establishment and grain yields in Mediterranean-like environments.
Epostane and indomethacin actions on ovarian kallikrein and plasminogen activator activities during ovulation in the gonadotropin-primed immature rat.

Kallikrein and plasminogen activator (PA) are serine proteases that have been implicated in the ovulatory process. Epostane and indomethacin are anti-ovulatory agents that inhibit steroid and eicosanoid synthesis, respectively. This study examines the effects of these two anti-ovulatory agents on ovarian kallikrein and PA activities during ovulation. The proteases were assayed by their actions on chromogenic peptide substrates S-2266 and S-2251, respectively. The ovulatory process was induced in 25-day-old Wistar rats by giving them hCG (10 IU, s.c.) 2 days after the animals had been primed with eCG (10 IU, s.c.). Control animals ovulated approximately 60-70 ova/rat, with the first ova appearing in the oviducts at 10-12 h after hCG administration, and this was the same time ovarian kallikrein and PA activities reached a peak. When doses of epostane ranging from 0.1-5.0 mg/rat or doses of indomethacin ranging from 0.03 to 3.16 mg/rat were administered s.c. at 3 h after hCG, the two drugs inhibited ovulation and ovarian kallikrein and PA activities in a dose-dependent manner. However, the anti-ovulatory action of the two drugs was more closely correlated with suppression of kallikrein activity than with PA activity. Treatment of the animals with exogenous progesterone reversed the inhibitory action of epostane, but not of indomethacin. The results suggest that the increase in ovarian progesterone at the time of ovulation may influence ovarian kallikrein and PA activities.
Voronoi-based aggregate nearest neighbor query processing in road networks

Aggregate nearest neighbor (ANN) query returns a common interesting data object that minimizes an aggregate distance for multiple query points. In this paper, we propose a novel approach to efficiently process ANN queries in road networks. This approach includes two processes: initializing process and pruning process, which are both based on computing kNN of query points in network Voronoi diagram. Experimental results show that our approach outperforms existing approaches on both response time and page accesses.