Miracle-dz/UniFAR-FTUs huggingface dataset:
Scd 5 p and Clathrin Function Are Important for Cortical Actin Organization , Endocytosis , and Localization of Sla 2 p in Yeast

SCD5 was identified as a multicopy suppressor of clathrin HC-deficient yeast. SCD5 is essential, but an scd5338 mutant, expressing Scd5p with a C-terminal truncation of 338 amino acids, is temperature sensitive for growth. Further studies here demonstrate that scd5338 affects receptor-mediated and fluid-phase endocytosis and normal actin organization. The scd5338 mutant contains larger and depolarized cortical actin patches and a prevalence of G-actin bars. scd5338 also displays synthetic negative genetic interactions with mutations in several other proteins important for cortical actin organization and endocytosis. Moreover, Scd5p colocalizes with cortical actin. Analysis has revealed that clathrin-deficient yeast also have a major defect in cortical actin organization and accumulate G-actin. Overexpression of SCD5 partially suppresses the actin defect of clathrin mutants, whereas combining scd5338 with a clathrin mutation exacerbates the actin and endocytic phenotypes. Both Scd5p and yeast clathrin physically associate with Sla2p, a homologue of the mammalian huntingtin interacting protein HIP1 and the related HIP1R. Furthermore, Sla2p localization at the cell cortex is dependent on Scd5p and clathrin function. Therefore, Scd5p and clathrin are important for actin organization and endocytosis, and Sla2p may provide a critical link between clathrin and the actin cytoskeleton in yeast, similar to HIP1(R) in animal cells.
Sulphur Abundance in Very Metal-poor Stars

We have obtained high-resolution high signal-to-noise ratio spectra of the S I near-infrared doublet at 8694 Å in eight metal-poor stars with metallicities in the range -0.6 ≤ [Fe/H] ≤ -3.0. Elemental sulphur abundances were derived for six targets, and upper limits were set for two of the most metal-poor stars in the sample. The sulphur doublet at 8694.62 Å has been detected in three stars (HD 19445, HD 2665, and HD 2796) with [Fe/H] ≤ -1.9. Our observations, combined with those available in the literature, indicate a monotonic increase of the [S/Fe] ratio as [Fe/H] decreases, reaching values of [S/Fe] ~ 0.7-0.8 below [Fe/H] = -2. We discuss plausible scenarios for the interpretation of these results.
Amplifying-forward MIMO based mutual information analysis for correlated fading channel

The use of multiple antennas at source, relay and destination terminal promises huge increase in quality of services, capacity and high data rate. This paper presents the mutual information of Multiple-Input-Multiple-Output MIMO relay channels when perfect channel state Information (CSI) is assumed at relay and destination terminal, it may also include spatially correlated channels and correlated noise. The analytical expression of Mutual information (MI) generating function has been derived by implementing multiple copies of the Gaussian integration. When the number of antenna at the relay terminal is more compare to the source and destination terminal this significantly increase the mutual information of channel. It has been observed that there is significant increase in MI for the value of (Ms,Md,Mr) to be (3,3,4) as compared to (3,3,3). Whereas the response gets worse with (4,4,3) as compared to (4,4,4). The simulation result confirmed that analytical method produces accurate and improved result for antenna array in MIMO for two, three, four and more antenna combinations.
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine Retroperitoneal Packing as Part of Damage Control Surgery in a Danish Trauma Centre – Fast, Effective, and Cost-effective

Background: Retroperitoneal packing in patients with severe haemorrhage is a cornerstone of modern pelvic fracture management. However, few Danish trauma surgeons have experience with this procedure, and trauma audits show that many hesitate to perform the procedure, indicating a need for hands-on training for this simple and potentially lifesaving procedure.
Strategies to promote adherence to treatment by pulmonary tuberculosis patients: a systematic review

Objective:The objective of this study is to review and synthesise the best available research evidence that investigates the effectiveness of strategies to promote adherence to treatment by patients with newly diagnosed pulmonary tuberculosis (TB). Methods:The search sought to find published and unpublished studies. The search covered articles published from 1990 to 2010 in English and Thai. The database search included Cumulative Index to Nursing and Allied Health Literature (CINAHL), EMBASE, Cochrane Library, PubMed, Science Direct, Current Content Connect, Thai Nursing Research Database, Thai thesis database, Digital Library of Thailand Research Fund, Research of National Research Council of Thailand and Database of Office of Higher Education Commission. Studies were additionally identified from reference lists of all studies retrieved. Eligible studies were randomised controlled trials that explored different strategies to promote adherence to TB treatment of patients with newly diagnosed pulmonary TB and also included quasiexperimental studies. Two of the investigators independently assessed the studies and then extracted and summarised data from eligible studies. Extracted data were entered into Review Manager software and analysed. Results:A total of 7972 newly diagnosed pulmonary TB patients participated in 10 randomised controlled trials and eight quasiexperimental studies. The studies reported on the effectiveness of a number of specific interventions to improve adherence to TB treatment among newly diagnosed pulmonary TB patients. These interventions included directly observed treatment (DOT) coupled with alternative patient supervision options, case management with DOT, short-course directly observed treatment, the intensive triad-model programme and an intervention package aimed at improved counselling and communication, decentralisation of treatment, patient choice of a DOT supporter and reinforcement of supervision activities. Conclusion:This review found evidence of beneficial effects from the DOT with regard to the medication adherence among TB patients in terms of cure rate and success rate. However, no beneficial effect was found from DOT intervention with increasing completion rate. In addition, the combined interventions to improve adherence to tuberculosis treatment included case management with directly observed treatment short-course program, the intensive triad-model programme and intervention package. These interventions should be implemented by healthcare providers and tailored to local contexts and circumstances, wherever appropriate.
Clusterfile: a flexible physical layout parallel file system

This paper presents Clusterfile, a parallel file system that provides parallel file access on a cluster of computers. We introduce a file partitioning model that has been used in the design of Clusterfile. The model uses a data representation that is optimized for multidimensional array partitioning while allowing arbitrary partitions. The paper shows how the file model can be employed for file partitioning into both physical subfiles and logical views. We also present how the conversion between two partitions of the same file is implemented using a general memory redistribution algorithm. We show how we use the algorithm to optimize non‐contiguous read and write operations. The experimental results include performance comparisons with the Parallel Virtual File System (PVFS) and an MPI‐IO implementation for PVFS. Copyright © 2003 John Wiley & Sons, Ltd.
The Changing Landscape of Total Laryngectomy Surgery

Objective To determine if the number of total laryngectomies (TL) performed in California has changed over a 15-year period from 1996 to 2010. We also sought to investigate if the location of surgery, length of stay, use of rotational/free flaps, cost, and complications changed over this period. Study Design Cross-sectional. Setting California Hospital Inpatient Discharge Data sets. Subjects and Methods All patient records enlisting International Classification of Diseases, Ninth Revision, Clinical Modification procedural codes for total/radical laryngectomy were extracted from 1996 to 2010 data sets. Patients’ demographics were evaluated. Hospitals were coded as university medical centers (UMCs) vs others. Population-adjusted surgery rates, percentage of surgeries with rotational/free flap and/or percutaneous endoscopic gastrostomy (PEG), length of stay, total charges, and disposition status were then analyzed and compared between UMCs and non-UMCs. Results A total of 4145 TLs were performed in California during 1996 to 2010. The overall number of surgeries declined from 1.3 to 0.6 per 100,000 California residents (P < .001). The number of surgeries performed in non-UMCs dropped by 70% during this period (P < .001). The median length of stay increased from 10 to 12 days, and the median total charges increased from $45,000 to $192,000 over the 15-year period. The use of rotational/free flaps more than doubled, and the use of PEG tubes increased 7-fold. Conclusion The total number of TLs in California has declined and surgeries are currently being performed equally at UMCs and non-UMCs. The use of rotational/free flaps, costs, hospital stay length, and complications have increased over the past 15 years.
Evolution of MicroRNA Genes in Oryza sativa and Arabidopsis thaliana: An Update of the Inverted Duplication Model

The origin and evolution of microRNA (miRNA) genes, which are of significance in tuning and buffering gene expressions in a number of critical cellular processes, have long attracted evolutionary biologists. However, genome-wide perspectives on their origins, potential mechanisms of their de novo generation and subsequent evolution remain largely unsolved in flowering plants. Here, genome-wide analyses of Oryza sativa and Arabidopsis thaliana revealed apparently divergent patterns of miRNA gene origins. A large proportion of miRNA genes in O. sativa were TE-related and MITE-related miRNAs in particular, whereas the fraction of these miRNA genes much decreased in A. thaliana. Our results show that the majority of TE-related and pseudogene-related miRNA genes have originated through inverted duplication instead of segmental or tandem duplication events. Based on the presented findings, we hypothesize and illustrate the four likely molecular mechanisms to de novo generate novel miRNA genes from TEs and pseudogenes. Our rice genome analysis demonstrates that non-MITEs and MITEs mediated inverted duplications have played different roles in de novo generating miRNA genes. It is confirmed that the previously proposed inverted duplication model may give explanations for non-MITEs mediated duplication events. However, many other miRNA genes, known from the earlier proposed model, were rather arisen from MITE transpositions into target genes to yield binding sites. We further investigated evolutionary processes spawned from de novo generated to maturely-formed miRNA genes and their regulatory systems. We found that miRNAs increase the tunability of some gene regulatory systems with low gene copy numbers. The results also suggest that gene balance effects may have largely contributed to the evolution of miRNA regulatory systems.
Bidirectional touch interaction for immaterial displays

In this paper, we present an immaterial mid-air display with touchscreen capability and aerial tactile feedback. By augmenting a projected desktop fogscreen display with a 3D camera for touch detection and a focusable, modulated ultrasonic phased array for tactile feedback generation, very convincing yet completely immaterial user interfaces were constructed. A typical feedback response is delivered within 2 ms with a positional resolution of 2 mm and an active spot size of 10 mm at 100 mm distance. By combining a number of the tileable ultrasonic arrays, a tighter focus with much higher intensity can be produced.
Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water.

Cellular distribution of cystic fibrosis transmembrane conductance regulator (CFTR) immunofluorescence was detected by monoclonal antibody directed to the C terminus of killifish CFTR (kfCFTR) in chloride cells of fresh water (FW) adapted fish and animals transferred to sea water (SW) for 24h, 48h and 14+ days. Confocal microscopy allowed localization within mitochondria-rich (MR) cells to be determined as superficial (i.e. in the apical membrane) or deeper within the cytoplasm of the cells. In FW, 90 % of MR cells had diffuse kfCFTR immunofluorescence in the central part of the cytosol, with only 8.1 % having apical kfCFTR, which was 6.6+/-0.54 microm below the microridges of surrounding pavement cells. Curiously, FW but not SW pavement cells also had positive immunofluorescence to kfCFTR. After 24h in SW, a time when kfCFTR expression is elevated, a condensed punctate immunofluorescence appeared among 18.8 % of MR cells, 13.4+/-0.66 microm (mean +/- S.E.M.) below the surface of the cells. By 48h, a majority (76.3 %) of MR cells had punctate kfCFTR distribution and the distance from the surface was less (7.8+/-0.2 microm), a distribution approaching the SW-acclimated condition (i.e. all MR cells showing kfCFTR immunofluorescence, 6.1+/-0.04 microm below the surface). In contrast, NKCC immunofluorescence was condensed and localized in lateral parts of MR cell complexes in FW animals and then redistributed to the whole basal cytoplasm after acclimation to SW. CFTR, the anion channel responsible for Cl(-) secretion in marine teleosts, redistributes in MR cells during SW acclimation by condensation of a diffuse distribution below the apical crypt, followed by translocation and insertion in the apical membrane. NKCC, the cotransporter that translocates Cl(-) across the basolateral membrane, moves from an eccentric cytosolic location in FW to a diffuse basolateral localization in SW chloride cells.
Reliability of Power Output During Eccentric Sprint Cycling

Abstract Brughelli, M and Van Leemputte, M. Reliability of power output during eccentric sprint cycling. J Strength Cond Res 27(1): 76–82, 2013—The purpose of this study was to determine the reliability of power outputs during maximal intensity eccentric cycling over short durations (i.e., eccentric sprint cycling) on a “motor-driven” isokinetic ergometer. Fourteen physically active male subjects performed isokinetic eccentric cycling sprints at 40, 60, 80, 100, and 120 revolutions per minute (rpm) on 4 separate occasions (T1–T4). Each sprint lasted for 6 seconds, and absolute measures of mean power (MP) and peak power (PP) per revolution were recorded. Significant increases in MP and PP were observed between T1 and subsequent trials, but no significant differences were identified between T2, T3, and T4. The coefficient of variation (CV) and intraclass correlation coefficient (ICC) were calculated to reflect within-subject and between-session reliability of MP and PP at each cadence. The CV improved to below 10% for cadences of 60, 80, 100, and 120 rpm between T3 and T4, and the majority of ICC values improved to above 0.90. The remaining ICC values remained in the moderate range between T3 and T4 (i.e., 0.82–0.89). Coefficient of variation and ICC values for the 40 rpm cadence remained at unacceptable levels throughout the 4 trials and thus should be avoided in future investigations. The results of this study indicate that reliable power outputs may be obtained after 2 familiarization sessions during eccentric sprint cycling at cadences ranging from 60 to 120 rpm.
TSP1–CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction

Aims Thrombospondin-1 (TSP1) is a ligand for CD47 and TSP1−/− mice are protected from pulmonary hypertension (PH). We hypothesized the TSP1–CD47 axis is upregulated in human PH and promotes pulmonary arterial vasculopathy. Methods and results We analyzed the molecular signature and functional response of lung tissue and distal pulmonary arteries (PAs) from individuals with (n = 23) and without (n = 16) PH. Compared with controls, lungs and distal PAs from PH patients showed induction of TSP1–CD47 and endothelin-1/endothelin A receptor (ET-1/ETA) protein and mRNA. In control PAs, treatment with exogenous TSP1 inhibited vasodilation and potentiated vasoconstriction to ET-1. Treatment of diseased PAs from PH patients with a CD47 blocking antibody improved sensitivity to vasodilators. Hypoxic wild type (WT) mice developed PH and displayed upregulation of pulmonary TSP1, CD47, and ET-1/ETA concurrent with down regulation of the transcription factor cell homolog of the v-myc oncogene (cMyc). In contrast, PH was attenuated in hypoxic CD47−/− mice while pulmonary TSP1 and ET-1/ETA were unchanged and cMyc was overexpressed. In CD47−/− pulmonary endothelial cells cMyc was increased and ET-1 decreased. In CD47+/+ cells, forced induction of cMyc suppressed ET-1 transcript, whereas suppression of cMyc increased ET-1 signaling. Furthermore, disrupting TSP1–CD47 signaling in pulmonary smooth muscle cells abrogated ET-1-stimulated hypertrophy. Finally, a CD47 antibody given 2 weeks after monocrotaline challenge in rats upregulated pulmonary cMyc and improved aberrations in PH-associated cardiopulmonary parameters. Conclusions In pre-clinical models of PH CD47 targets cMyc to increase ET-1 signaling. In clinical PH TSP1–CD47 is upregulated, and in both, contributes to pulmonary arterial vasculopathy and dysfunction.
Low-Cost Concurrent Error Detection for Floating-Point Unit (FPU) Controllers

We present a nonintrusive concurrent error detection (CED) method for protecting the control logic of a contemporary floating-point unit (FPU). The proposed method is based on the observation that control logic errors lead to extensive data path corruption and affect, with high probability, the exponent part of the IEEE-754 floating-point representation. Thus, exponent monitoring can be utilized to detect errors in the control logic of the FPU. Predicting the exponent involves relatively simple operations; therefore, our method incurs significantly lower overhead than the classical approach of duplicating the control logic of the FPU. Indeed, experimental results on the openSPARC T1 processor using SPEC2006FP benchmarks show that as compared to control logic duplication, which incurs an area overhead of 17.9 percent of the FPU size, our method incurs an area overhead of only 5.8 percent yet still achieves detection of over 93 percent of transient errors in the FPU control logic. Moreover, the proposed method offers the ancillary benefit of also detecting 98.1 percent of the data path errors that affect the exponent, which cannot be detected via duplication of control logic. Finally, when combined with a classical residue code-based method for the fraction, our method leads to a complete CED solution for the entire FPU which provides a coverage of 94.1 percent of all errors at an area cost of 16.32 percent of the FPU size.
Topoisomerase Mutations Associated with In Vitro Selection of Resistance to Moxifloxacin in Streptococcus pneumoniae

ABSTRACT We analyzed the frequencies of selection, the order of acquisition, and the mutations selected on moxifloxacin in two wild-type pneumococcal strains, R6 and 5714. The first selection step showed either a single GyrA mutation or no mutation in any of the quinolone resistance-determining regions. Second-step mutants selected had either a second mutation in ParC or in ParE. Moxifloxacin could belong to these fluoroquinolones, which preferentially target GyrA though probably acting equally through both gyrase and topoisomerase IV.
Estimating Mean First Passage Time of Biased Random Walks with Short Relaxation Time on Complex Networks

Biased random walk has been studied extensively over the past decade especially in the transport and communication networks communities. The mean first passage time (MFPT) of a biased random walk is an important performance indicator in those domains. While the fundamental matrix approach gives precise solution to MFPT, the computation is expensive and the solution lacks interpretability. Other approaches based on the Mean Field Theory relate MFPT to the node degree alone. However, nodes with the same degree may have very different local weight distribution, which may result in vastly different MFPT. We derive an approximate bound to the MFPT of biased random walk with short relaxation time on complex network where the biases are controlled by arbitrarily assigned node weights. We show that the MFPT of a node in this general case is closely related to not only its node degree, but also its local weight distribution. The MFPTs obtained from computer simulations also agree with the new theoretical analysis. Our result enables fast estimation of MFPT, which is useful especially to differentiate between nodes that have very different local node weight distribution even though they share the same node degrees.
Dependency Between Error Variance of the a Priori Information and a Modified Channel Noise Variance in Turbo-Equalisation

In this paper, we propose a method based on the modification of the channel noise variance at the equaliser input in order to improve the performance of a turbo-equaliser. We show a relation between the modified channel noise variance and the a priori information statistics. The simulation are done for 2 types of turbo-equalisers
A Study of Ventricular Arrhythmias Associated with Acute Myocardial Infarction in the Canine Heart

A study was designed to correlate changes in bipolar epicardial electrograms recorded from normal and acutely infarcted myocardium with the onset of ventricular arrhythmias. Electrodes were sewn to selected sites on the left ventricle, and after control electrograms and ECG's were recorded, the left anterior descending coronary artery was doubly ligated close to its origin. Electrograms recorded from within the infarct initially manifested diminished amplitude and increased duration of the deflection which reflected depolarization. The appearance of ventricular arrhythmias during phase 1 of Harris was associated with the appearance in electrograms recorded from within the infarct of continuous electrical activity which extended beyond the T wave of the preceding beat and preceded the onset of the arrhythmia. During the continuous electrical activity, it was not possible to delineate which deflections of the electrogram reflected depolarization or repolarization and it was suggested that the continuous electrical activity was localized fibrillation. For three experiments in which no arrhythmias occurred during phase 1 of Harris and for all animals which survived into phase 2 of Harris, the absence of ventricular arrhythmias was correlated with the absence of continuous electrical activity or locallized fibrillation recorded from within the area of the infarct.
ATTITUDES AND SOCIAL COGNITION A Probabilistic Contrast Model of Causal Induction

Deviations from the predictions of covariational models of causal attribution have often been reported in the literature. These include a bias against using consensus information, a bias toward attributing effects to a person, and a tendency to make a variety of unpredicted conjunctive attributions. It is contended that these deviations, rather than representing irrational biases, could be due to (a) unspecified information over which causal inferences are computed and (b) the questionable normativeness of the models against which these deviations have been measured. A probabilistic extension of Kelley's analysis-of-variance analogy is proposed. An experiment was performed to assess the above biases and evaluate the proposed model against competing ones. The results indicate that the inference process is unbiased.