Miracle-dz/UniFAR-FTUs huggingface dataset:
Pretreatment Hematologic Findings as Novel Predictive Markers for Facial Palsy Prognosis

Objective To examine the relationship between prognosis of 2 different facial palsies and pretreatment hematologic laboratory values. Study Design Multicenter case series with chart review. Setting Three tertiary care hospitals. Subjects and Methods We examined the clinical records of 468 facial palsy patients who were treated with an antiviral drug in combination with either oral or intravenous corticosteroids in participating hospitals between 2010 and 2014. Patients were divided into a Bell’s palsy group or a Hunt’s palsy group. We used the Yanagihara facial nerve grading system to grade the severity of facial palsy. “Recovery” from facial palsy was defined as achieving a Yanagihara score ≥36 points within 6 months of onset and having no accompanying facial contracture or synkinesis. We collected information about pretreatment hematologic findings, demographic data, and electrophysiologic test results of the Bell and Hunt group patients who recovered and those who did not. We then compared these data across the 2 palsy groups. Results In the Bell’s palsy group, recovered and unrecovered patients differed significantly in age, sex, electroneuronography score, stapedial muscle reflex, neutrophil rate, lymphocyte rate, neutrophil-to-lymphocyte ratio, and initial Yanagihara score. In the Hunt’s palsy group, recovered and unrecovered patients differed in age, electroneuronography score, stapedial muscle reflex, monocyte rate, platelet count, mean corpuscular volume, and initial Yanagihara score. Conclusions Pretreatment hematologic findings, which reflect the severity of inflammation and bone marrow dysfunction caused by a virus infection, are useful for predicting the prognosis of facial palsy.
Tiotropium bromide inhibits TGF-β-induced MMP production from lung fibroblasts by interfering with Smad and MAPK pathways in vitro

Background: Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation and structural alterations (ie, tissue remodeling) throughout the conducting airways, parenchyma, and pulmonary vasculature. Matrix metalloproteinases (MMPs) are extracellular degrading enzymes that play a critical role in inflammatory cell infiltration and tissue remodeling, but the influence of the agents that are used for the treatment of COPD on the production of MMPs is not well understood. Purpose: The present study aimed to examine the influence of tiotropium bromide hydrate (TBH) on the production of MMPs from lung fibroblasts (LFs) induced by transforming growth factor (TGF)-β in vitro. Methods: LFs, at a concentration of 5 × 105 cells·mL−1, were stimulated with TGF-β in the presence of various concentrations of TBH. MMP-1 and MMP-2 levels in culture supernatants were examined by enzyme-linked immunosorbent assay (ELISA), and MMP messenger ribonucleic acid (mRNA) expression was examined by real-time polymerase chain reaction (RT-PCR). The influence of TBH on TGF-β signaling pathways was also analyzed by examining Smad activation and signaling protein phosphorylation by ELISA. Results: TBH at more than 15 pg·mL−1 inhibited the production of MMP-1 and MMP-2, but not tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2, from LFs, after TGF-β stimulation. TBH also suppressed MMP mRNA expression through the inhibition of Smad activation and signaling protein, extracellular-signal-regulated kinase (ERK) 1 and 2, and c-Jun N-terminal kinase (JNK), phosphorylation. Conclusion: These results may suggest that TBH suppresses MMP production from LFs, through interference of TGF-β-mediated signaling pathways and results in favorable modification of the clinical status of COPD.
The bending of sliding microtubules imaged by confocal light microscopy and negative stain electron microscopy

Summary Individual microtubules can be visualised by confocal microscopy in reflection mode; when associated with a glass surface, they show up as black lines against the bright reflection from the surface. The high contrast imaging allows details of the behaviour of sliding microtubules to be studied easily. Taxol-stabilised microtubules sliding over kinesin-coated surfaces are normally straight, but can bend into tight loops if the leading end sticks to the surface. Some remain curved after release and move in circles. In such cases, the microtubule lattice must have become stably deformed. Electron microscopy of microtubules fixed during sliding shows no gross rearrangement of the subunit lattice and indicates that microtubule bending is mainly achieved by increased twisting of the longitudinal protofilaments around the microtubule.
Neuromagnetic correlates of temporal gap detection in human auditory cortex

In this study, spatiotemporal profiles of brain responses to gaps in tones were investigated using magnetoencephalography (MEG). Participants listened passively to diotically presented stimuli consisting of leading and trailing markers with gaps of 0, 30, or 80 ms in duration that were inserted between the two markers. The leading and trailing markers were 300-ms pure tones of either 800 or 3200 Hz in frequency. For the withinfrequency condition, the leading and trailing markers were identical in frequency, whereas for the between-frequency condition, the leading marker and the trailing marker were presented at different frequencies from each other. Neuromagnetic brain activities were recorded from 6 participants. Auditory evoked fields (AEFs) were obtained from temporal areas in both hemispheres. Auditory MEG responses (N100m) appeared in response to the onset of the trailing markers as well as the leading markers. In the between-frequency conditions, N100m appeared for all trailing marker onsets regardless of the gap duration. In within-frequency conditions, the stimulus did not produce a clear N100m response when there was no gap. Even though N100m appeared with gap durations of 30 ms and 80 ms, the amplitudes were smaller than those observed in the between-frequency conditions. Minimum norm estimate (MNE) was applied to localize the activated areas for these conditions. The results indicated that the areas activated by an 800-Hz tone were distributed more posteriorly than those for a 3200-Hz tone.
Development of biocompatible materials for use in myringoplasty

Tympanic membrane (TM) perforations lead to significant hearing loss and may promote infection of the middle ear (ME). Myringoplasty is commonly performed to repair chronic perforations, with the primary goals of perforation closure and hearing restoration. Although various grafts and materials have been used to promote TM regeneration, all have associated limitations. Recent experimental studies have shown the potential of silk fibroin scaffold (SFS) and porcine-derived acellular collagen type I/III scaffold (ACS) as onlay graft materials for TM perforation repair. The aims of this study were to evaluate the in vivo safety and efficacy of SFS and ACS, compared with two commonly used graft materials (paper patch and Gelfoam) for the promotion of TM regeneration. In this thesis, a general overview of different types of biological and synthetic scaffolds for TM regeneration was provided, and evidence of scaffolds’ function in TM wound healing was described. Furthermore, a brief review of ME packing agents and a discussion on the potential benefits of no packing also was supplied. Firstly, the in vivo biocompatibility of SFS and ACS were characterised and compared with Gelfoam and paper in a rat model. The scaffolds were implanted in subcutaneous (SC) tissue and ME cavity followed by histological and otoscopic evaluation for up to 26 weeks. Our results revealed that SFS and ACS were well tolerated and compatible in rat SC and ME
Clonal Amniotic Fluid-Derived Stem Cells Express Characteristics of Both Mesenchymal and Neural Stem Cells1

Abstract Recent evidence has shown that amniotic fluid may be a novel source of fetal stem cells for therapeutic transplantation. We previously developed a two-stage culture protocol to isolate a population of amniotic fluid-derived mesenchymal stem cells (AFMSCs) from second-trimester amniocentesis. AFMSCs maintain the capacity to differentiate into multiple mesenchymal lineages and neuron-like cells. It is unclear whether amniotic fluid contains heterogeneous populations of stem cells or a subpopulation of primitive stem cells that are similar to marrow stromal cells showing the behavior of neural progenitors. In this study, we showed a subpopulation of amniotic fluid-derived stem cells (AF-SCs) at the single-cell level by limiting dilution. We found that NANOG- and POU5F1 (also known as OCT4)-expressing cells still existed in the expanded single cell-derived AF-SCs. Aside from the common mesenchymal characteristics, these clonal AF-SCs also exhibit multiple phenotypes of neural-derived cells such as NES, TUBB3, NEFH, NEUNA60, GALC, and GFAP expressions both before and after neural induction. Most importantly, HPLC analysis showed the evidence of dopamine release in the extract of dopaminergic-induced clonal AF-SCs. The results of this study suggest that besides being an easily accessible and expandable source of fetal stem cells, amniotic fluid will provide a promising source of neural progenitor cells that may be used in future cellular therapies for neurodegenerative diseases and nervous system injuries.
Determination of the Surface Roughness Values of Turkish Red Pine (Pinus brutia (Ten.)) Woods

The aim of this study was to determine the surface roughness values of Turkish red pine samples obtained from the seven natural growth areas in Turkey. The samples were cut with a circular saw, planed with a thickness machine, and sanded with a sanding machine (with No. 80 sandpaper). After the samples were processed as radial and tangential surfaces in the machines, their surface roughness values (Ra, Ry, and Rz) were measured in accordance with ISO 4288 (1996). According to the statistical results, the lowest surface roughness values were in the samples obtained from the Mugla and Samsun areas on the tangential surfaces that were processed with the thickness machine.
Is There a Curse of Dimensionality for Contraction Fixed Points in the Worst Case

This paper analyzes the complexity of the contraction fixed point problem: compute an e-approximation to the fixed point V * = Γ(V * ) of a contraction mapping r that maps a Banach space B d of continuous functions of d variables into itself. We focus on quasi linear contractions where Γ is a nonlinear functional of a finite number of conditional expectation operators. This class includes contractive Fredholm integral equations that arise in asset pricing applications and the contractive Bellman equation from dynamic programming. In the absence of further restrictions on the domain of Γ, the quasi linear fixed point problem is subject to the curse of dimensionality, i.e., in the worst case the minimal number of function evaluations and arithmetic operations required to compute an e-approximation to a fixed point V * e B d increases exponentially in d. We show that the curse of dimensionality disappears if the domain of Γ has additional special structure. We identify a particular type of special structure for which the problem is strongly tractable even in the worst case, i.e., the number of function evaluations and arithmetic operations needed to compute an e-approximation of V * is bounded by Ce -p where C and p are constants independent of d. We present examples of economic problems that have this type of special structure including a class of rational expectations asset pricing problems for which the optimal exponent p = 1 is nearly achieved.
High-Throughput Microplate-Based Assay to Monitor Plasma Membrane Wounding and Repair

The plasma membrane of mammalian cells is susceptible to disruption by mechanical and biochemical damages that frequently occur within tissues. Therefore, efficient and rapid repair of the plasma membrane is essential for maintaining cellular homeostasis and survival. Excessive damage of the plasma membrane and defects in its repair are associated with pathological conditions such as infections, muscular dystrophy, heart failure, diabetes, and lung and neurodegenerative diseases. The molecular events that remodel the plasma membrane during its repair remain poorly understood. In the present work, we report the development of a quantitative high-throughput assay that monitors the efficiency of the plasma membrane repair in real time using a sensitive microplate reader. In this assay, the plasma membrane of living cells is perforated by the bacterial pore-forming toxin listeriolysin O and the integrity and recovery of the membrane are monitored at 37°C by measuring the fluorescence intensity of the membrane impermeant dye propidium iodide. We demonstrate that listeriolysin O causes dose-dependent plasma membrane wounding and activation of the cell repair machinery. This assay was successfully applied to cell types from different origins including epithelial and muscle cells. In conclusion, this high-throughput assay provides a novel opportunity for the discovery of membrane repair effectors and the development of new therapeutic compounds that could target membrane repair in various pathological processes, from degenerative to infectious diseases.
Two Crystal Structures of Bombyx mori Lipoprotein 3 - Structural Characterization of a New 30-kDa Lipoprotein Family Member

The 30-kDa family of lipoproteins from insect hemolymph has been the focus of a number of studies over the last few years. Recently, four crystal structures of Bombyx mori lipoprotein 7 have been determined. Here we report two crystal structures of another member of the 30-kDa lipoprotein family, Bombyx mori lipoprotein 3 (Bmlp3). The protein was isolated from its natural source, mulberry silkworm hemolymph. It crystallized in two different crystal forms, Bmlp3-p21 (space group P21) and Bmlp3-c2 (space group C2). The crystal structures were solved by molecular replacement using the coordinates of Bmlp7 as a starting model. The crystals of Bmlp3-p21 diffracted X-rays to 2.4 Å resolution and of Bmlp3-c2 to 2.1 Å resolution. Bmlp3 has an overall fold characteristic of 30-kDa lipoproteins, with a VHS-type N-terminal domain and β-trefoil C-terminal domain. Structural comparison of Bmlp3 and Bmlp7 shows that the loops present in the C-terminal domain are flexible and participate in dimer formation. Additionally, new putative binding sites of Bmlp3 have been analyzed in detail and the electrostatic potential of the protein surface at physiological pH 7.4 conditions has been calculated. The results of these calculations are the starting point for an explanation of the recently reported cell-penetrating properties of the 30-kDa lipoproteins.
Catastrophic Die-Off of Peary Caribou on the Western Queen Elizabeth Islands, Canadian High Arctic

The Peary caribou ( Rangifer tarandus pearyi ) is an endangered species in Canada, having been in an overall decline since 1961. Sightings of Peary caribou were compared from two aerial searches, in 1993 and 1998, on Bathurst and its neighbouring islands, western Queen Elizabeth Islands in the Canadian High Arctic. The comparison indicated a near-total (98%) cataclysmic decline in the number of Peary caribou seen per unit of search effort. In summer 1993, 2400 caribou were counted during 33.8 h of low-level helicopter searches. In contrast, in summer 1998, only 43 caribou were seen within the same area during 35.2 h of low-level helicopter searches. The frequency of observation was markedly different: 118.3 caribou/100 min in 1993, but only 2.0 caribou/100 min in 1998. The number of carcasses indicated that the decline resulted from deaths and not from mass emigration. Males died at a disproportionately higher rate than females among all 1+ yr old caribou, and bulls (4+ yr) compared to cows (3+ yr) had died at an even greater rate. Widespread, prolonged, exceptionally severe snow and ice conditions from 1994-95 to 1996-97 caused the die-off. Trends in snowfall are consistent with predictions for global warming in the western Canadian High Arctic. Future climate change may increase the frequency of years with unfavorable snow and ice conditions, which could prevent or at least impede future recovery of Peary caribou populations on the western Queen Elizabeth Islands, particularly to sizes that would support subsistence harvesting.
Emotion Regulation Moderates the Association between Empathy and Prosocial Behavior

Theory and evidence suggest that empathy is an important motivating factor for prosocial behaviour and that emotion regulation, i.e. the capacity to exert control over an emotional response, may moderate the degree to which empathy is associated with prosocial behaviour. However, studies to date have not simultaneously explored the associations between different empathic processes and prosocial behaviour, nor whether different types of emotion regulation strategies (e.g. cognitive reappraisal and expressive suppression) moderate associations between empathy and prosocial behaviour. One hundred–and-ten healthy adults completed questionnaire measures of empathy, emotion regulation and prosocial tendencies. In this sample, both affective and cognitive empathy predicted self-reported prosocial tendencies. In addition, cognitive reappraisal moderated the association between affective empathy and prosocial tendencies. Specifically, there was a significant positive association between empathy and prosocial tendencies for individuals with a low or average tendency to reappraise but not for those with a high tendency to reappraise. Our findings suggest that, in general, empathy is positively associated with prosocial behaviour. However, this association is not significant for individuals with a high tendency for cognitive reappraisal.
Major Reduction in Anti-Malarial Drug Consumption in Senegal after Nation-Wide Introduction of Malaria Rapid Diagnostic Tests

Background While WHO recently recommended universal parasitological confirmation of suspected malaria prior to treatment, debate has continued as to whether wide-scale use of rapid diagnostic tests (RDTs) can achieve this goal. Adherence of health service personnel to RDT results has been poor in some settings, with little impact on anti-malarial drug consumption. The Senegal national malaria control programme introduced universal parasite-based diagnosis using malaria RDTs from late 2007 in all public health facilities. This paper assesses the impact of this programme on anti-malarial drug consumption and disease reporting. Methods and Findings Nationally-collated programme data from 2007 to 2009 including malaria diagnostic outcomes, prescription of artemisinin-based combination therapy (ACT) and consumption of RDTs in public health facilities, were reviewed and compared. Against a marked seasonal variation in all-cause out-patient visits, non-malarial fever and confirmed malaria, parasite-based diagnosis increased nationally from 3.9% of reported malaria-like febrile illness to 86.0% over a 3 year period. The prescription of ACT dropped throughout this period from 72.9% of malaria-like febrile illness to 31.5%, reaching close equivalence to confirmed malaria (29.9% of 584873 suspect fever cases). An estimated 516576 courses of inappropriate ACT prescription were averted. Conclusions The data indicate high adherence of anti-malarial prescribing practice to RDT results after an initial run-in period. The large reduction in ACT consumption enabled by the move from symptom-based to parasite-based diagnosis demonstrates that effective roll-out and use of malaria RDTs is achievable on a national scale through well planned and structured implementation. While more detailed information on management of parasite-negative cases is required at point of care level to assess overall cost-benefits to the health sector, considerable cost-savings were achieved in ACT procurement. Programmes need to be allowed flexibility in management of these funds to address increases in other programmatic costs that may accrue from improved diagnosis of febrile disease.
A new classifier-based strategy for in-silico ion-channel cardiac drug safety assessment

There is currently a strong interest in using high-throughput in-vitro ion-channel screening data to make predictions regarding the cardiac toxicity potential of a new compound in both animal and human studies. A recent FDA think tank encourages the use of biophysical mathematical models of cardiac myocytes for this prediction task. However, it remains unclear whether this approach is the most appropriate. Here we examine five literature data-sets that have been used to support the use of four different biophysical models and one statistical model for predicting cardiac toxicity in numerous species using various endpoints. We propose a simple model that represents the balance between repolarisation and depolarisation forces and compare the predictive power of the model against the original results (leave-one-out cross-validation). Our model showed equivalent performance when compared to the four biophysical models and one statistical model. We therefore conclude that this approach should be further investigated in the context of early cardiac safety screening when in-vitro potency data is generated.
Global Existence for a Translating Near-Circular Hele-Shaw Bubble with Surface Tension

This paper concerns global existence for arbitrary nonzero surface tension of bubbles in a Hele–Shaw cell that translate in the presence of a pressure gradient. When the cell width to bubble size is sufficiently large, we show that a unique steady translating near-circular bubble symmetric about the channel centerline exists, where the bubble translation speed in the laboratory frame is found as part of the solution. We prove global existence for symmetric sufficiently smooth initial conditions close to this shape and show that the steady translating bubble solution is an attractor within this class of disturbances. In the absence of side walls, we prove stability of the steady translating circular bubble without restriction on symmetry of initial conditions. These results hold for any nonzero surface tension despite the fact that a local planar approximation for the steady shape near the front of the bubble would suggest Saffman–Taylor instability. We exploit a boundary integral approach that is particul.