Miracle-dz/UniFAR-FTUs huggingface dataset:
Travellers’ intention to purchase travel online: integrating trust and risk to the theory of planned behaviour

Abstract This study aims to determine which factors influence travellers’ intentions to purchase travel online by proposing and empirically testing a new model grounded on the theory of planned behaviour. In order to validate the model, a web-based questionnaire was applied and a total of 1732 valid responses were obtained. The findings show that attitudes, perceived risk, and perceived behavioural control have significant effects on intentions to purchase travel online. However, contrary to what was expected, neither trust nor the influence of others seems to directly affect intentions to purchase travel online. Finally, the paper discusses the findings with the implications for theory and practice and makes several suggestions for future research.
Simulating cosmic rays in clusters of galaxies – III. Non-thermal scaling relations and comparison to observations

Complementary views of galaxy clusters in the radio synchrotron, hard X-ray inverse Compton and high-energy γ-ray regimes are critical in calibrating them as high-precision cosmological probes. We present predictions for scaling relations between cluster mass and these non-thermal observables. To this end, we use high-resolution simulations of a sample of galaxy clusters spanning a mass range of almost two orders of magnitudes, and follow self-consistent cosmic ray physics on top of the radiative hydrodynamics. We model relativistic electrons that are accelerated at cosmological structure formation shocks and those that are produced in hadronic interactions of cosmic rays with ambient gas protons. Calibrating the magnetic fields of our model with Faraday rotation measurements, the synchrotron emission of our relativistic electron populations matches the radio synchrotron luminosities and morphologies of observed giant radio haloes and minihaloes surprisingly well. Using the complete sample of the brightest X-ray clusters observed by ROSAT in combination with our γ-ray scaling relation, we predict GLAST to detect about ten clusters allowing for Eddington bias due to the scatter in the scaling relation. The expected brightest γ-ray clusters are Ophiuchus, Fornax, Coma, A3627, Perseus and Centaurus. The high-energy γ-ray emission above 100 MeV is dominated by pion decays resulting from hadronic cosmic ray interactions. We provide an absolute lower flux limit for the γ-ray emission of Coma in the hadronic model which can be made tighter for magnetic field values derived from rotation measurements to match the GLAST sensitivity, providing thus a unique test for the possible hadronic origin of radio haloes. Our predicted hard X-ray emission, due to inverse Compton emission of shock accelerated and hadronically produced relativistic electrons, falls short of the detections in Coma and Perseus by a factor of 50. This casts doubts on inverse Compton interpretation and reinforces the known discrepancy of magnetic field estimates from Faraday rotation measurements and those obtained by combining synchrotron and inverse Compton emission.
The Palliative Role of Green Space for Somali Bantu Women Refugees in Displacement and Resettlement

ABSTRACT This article investigates recollections of place attachments among a sample of Somali Bantu women refugees who have resettled in a town in New York State, USA. Using photo-elicitation and narrative interviews we explored their recollections of place attachments in Somalia, their home of origin, in Kenya, where they lived in refugee camps for more than a decade and in community gardens in New York State. We asked about memories of farms in Somalia, of gardens in the Kenyan camps and of the community gardens in resettlement because we were interested in the role of green space in their experiences of displacement and resettlement. Green spaces were important in all three locations but especially with respect to the therapeutic, restorative and spiritual capacities in the gardens in both the camps in Kenya and in resettlement in the United States. The results of this research suggest that emplacement and meaning making in green spaces through farms and gardens in Somalia, in camps, and in community gardens in resettlement served a variety of palliative functions.
The Female Pediatric Surgeon

Abstract In medical schools throughout Europe, women make up an increasing proportion of graduates entering the medical profession. Even though this phenomenon is also found in the surgical profession, women are still clearly underrepresented. However, it has been demonstrated that women are equally qualified as men, and are as eager as men to aim for a surgical career. In general, a career in surgery has significant lifestyle implications for both men and women. In particular, women meet challenges such as pregnancy, maternity, and responsibility for childcare that compete with pressures of time and expectations of productivity. Further complicating the situation, there is a huge complexity of national legislation dealing with maternity and parental leave within Europe. Owing to this legal inconsistency, a strong demand on targeted policies and guidelines has increased particularly among the surgical staff. The scarcity of female role models and mentors has also been discussed as a possible explanation for the underrepresentation of women in academic surgery. Even in the 21st century, the advancement of women into leading positions of academic surgery and major surgical societies is still limited. An updated view of leadership development, the promotion of female surgeons in academic surgery, and identifying barriers to women entering this field are crucial to correcting the existing gender inequities. This contribution aims to highlight the current situation of women in academic surgery, outline findings on gender disparities, and define persistent obstacles to the advancement of women in surgery. In addition, this review presents new possibilities and provides approaches to overcome the underrepresentation of female surgeons. In current literature, there is only little information concerning the situation of female pediatric surgeons. Therefore, this article mainly relies on available data on the female surgeon in general.
Prolotherapy: A Non-Invasive Approach to Lesions of the Glenoid Labrum; A Non-Controlled Questionnaire Based Study

Lesions of the glenoid labrum are a common cause of shoulder instability and a frequent finding in patients with shoulder pain. Management of these patients typically involves an attempt to avoid surgery through conservative treatment. However, there is currently a dearth of conservative options that promote labral healing. Regenerative injection therapies, including prolotherapy, have shown promise in the treatment of several musculoskeletal disorders, but have not previously been applied to glenoid labral tear. Here we review several important aspects of these lesions and present an initial case series of 33 patients with labral tear that were treated in our clinic with intra-articular injections of hypertonic dextrose. Patient-reported assessments were collected by questionnaire at a mean follow-up time of 16 months. Treated patients reported highly significant improvements with respect to pain, stiffness, range of motion, crunching, exercise and need for medication. All 31 patients who reported pain at baseline experienced pain relief, and all 31 who reported exercise impairment at baseline reported improved exercise capability. Patients reported complete relief of 69% of recorded symptoms. One patient reported worsening of some symptoms. Prolotherapy for glenoid labral tear appears to be a safe procedure that merits further investigation.
A Strong Regenerative Ability of Cardiac Stem Cells Derived From Neonatal Hearts

Background— Human cardiac stem cells (CSCs) promote myocardial regeneration in adult ischemic myocardium. The regenerative capacity of CSCs in very young patients with nonischemic congenital heart defects has not been explored. We hypothesized that isolated neonatal-derived CSCs may have a higher regenerative ability than adult-derived CSCs and might address the structural deficiencies of congenital heart disease. Methods and Results— Human specimens were obtained during routine cardiac surgical procedures from right atrial appendage tissue discarded from 2 age groups: neonates and adults patients. We developed a reproducible isolation method that generated cardiosphere-derived cells (CDCs), regardless of starting tissue weight or age. Neonatal-derived CDCs demonstrated increased number of cardiac progenitor cells expressing c-kit+, flk-1, and Islet-1 by flow cytometry and immunofluorescence. When transplanted into infarcted myocardium, neonatal-derived CDCs had a significantly higher ability to preserve myocardial function, prevent adverse remodeling, and enhance blood vessel preservation and/or formation when compared with adult-derived CDCs. Last, neonatal-derived CDCs were more cardiomyogenic than adult-derived CDCs when cocultured with neonatal cardiomyocytes and displayed enhanced angiogenic function compared with adult-derived CDCs. Conclusions— Neonatal-derived CDCs have a strong regenerative ability when compared with adult-derived CDCs that may depend on angiogenic cytokines and an increase prevalence of stem cells. This has important implications in the potential use of CDCs in future clinical trials.
A Slot Blot Immunoassay for Quantitative Detection of Plasmodium falciparum Circumsporozoite Protein in Mosquito Midgut Oocyst

There is still a need for sensitive and reproducible immunoassays for quantitative detection of malarial antigens in preclinical and clinical phases of vaccine development and in epidemiology and surveillance studies, particularly in the vector host. Here we report the results of sensitivity and reproducibility studies for a research-grade, quantitative enhanced chemiluminescent-based slot blot assay (ECL-SB) for detection of both recombinant Plasmodium falciparum circumsporozoite protein (rPfCSP) and native PfCSP from Oocysts (Pf Oocyst) developing in the midguts of Anopheles stephensi mosquitoes. The ECL-SB detects as little as 1.25 pg of rPfCSP (linear range of quantitation 2.5–20 pg; R2 = 0.9505). We also find the earliest detectable expression of native PfCSP in Pf Oocyst by ECL-SB occurs on day 7 post feeding with infected blood meal. The ECL-SB was able to detect approximately as few as 0.5 day 8 Pf Oocysts (linear quantitation range 1–4, R2 = 0.9795) and determined that one Pf Oocyst expressed approximately 2.0 pg (0.5–3 pg) of native PfCSP, suggesting a similar range of detection for recombinant and native forms of Pf CSP. The ECL-SB is highly reproducible; the Coefficient of Variation (CV) for inter-assay variability for rPf CSP and native PfCSP were 1.74% and 1.32%, respectively. The CVs for intra-assay variability performed on three days for rPf CSP were 2.41%, 0.82% and 2% and for native Pf CSP 1.52%, 0.57%, and 1.86%, respectively. In addition, the ECL-SB was comparable to microscopy in determining the P. falciparum prevalence in mosquito populations that distinctly contained either high and low midgut Pf Oocyst burden. In whole mosquito samples, estimations of positivity for P. falciparum in the high and low burden groups were 83.3% and 23.3% by ECL-SB and 85.7% and 27.6% by microscopy. Based on its performance characteristics, ECL-SB could be valuable in vaccine development and to measure the parasite prevalence in mosquitoes and transmission-blocking interventions in endemic areas.
Development of dietary soluble fibres by enzymatic synthesis and assessment of their digestibility in in vitro, animal and randomised clinical trial models

Abstract The aim was to develop novel fibres by enzymatic synthesis, to determine their total dietary fibre by AOAC method 2009.01 and to estimate their potential digestibility and assess their digestibility in vivo using glycaemic and insulinaemic responses as markers in mice and randomised clinical trial models. We found that fibre candidates to which α-(1,2) branching was added were resistant to digestion in the mouse model, depending on the amount of branching. These results show that in vivo models are needed to reliably assess the digestibility of α-glycosidic-linked oligomeric dietary fibre candidates, possibly due to absence of brush border α-glucosidase activity in the current in vitro assessment. α-(1,3)-linked and α-(1,6)-linked glucose oligomers were completely digested in humans and mice. In conclusion, it is possible to develop dietary soluble fibres by enzymatic synthesis. Adding α-(1,2) branching increases their resistance to digestion in vivo and can thus improve their suitability as potential fibre candidates. Clinical Trial Registry: ClinicalTrials.gov, NCT02701270
Robust Image Watermarking Using Independent Component Analysis

This paper proposes an image watermarking scheme for copyright protection based on Independent Component Analysis (ICA). In the suggested scheme, we divide the original image into blocks and use the ICA to project the image into a basis with its components as statistically independent as possible. The data embedding is carried out in cumulative form in the ICA basis and also the ICA is used for watermark extraction. The extraction process could be either non-blind, through a straightforward procedure, or blind, via a tricky method we propose. Experimental results show that the new method outperforms some well-known image watermarking methods [1], [2], [3] in spatial or frequency domain and is robust against various attacks, including noise addition, resizing, low pass filtering, multiple marks embedding, JPEG compression, gray-scale reduction, and cropping parts of image.
Predictive coding under the free-energy principle

This paper considers prediction and perceptual categorization as an inference problem that is solved by the brain. We assume that the brain models the world as a hierarchy or cascade of dynamical systems that encode causal structure in the sensorium. Perception is equated with the optimization or inversion of these internal models, to explain sensory data. Given a model of how sensory data are generated, we can invoke a generic approach to model inversion, based on a free energy bound on the model's evidence. The ensuing free-energy formulation furnishes equations that prescribe the process of recognition, i.e. the dynamics of neuronal activity that represent the causes of sensory input. Here, we focus on a very general model, whose hierarchical and dynamical structure enables simulated brains to recognize and predict trajectories or sequences of sensory states. We first review hierarchical dynamical models and their inversion. We then show that the brain has the necessary infrastructure to implement this inversion and illustrate this point using synthetic birds that can recognize and categorize birdsongs.
Enabling Reconfigurable Hardware Accelerators for the Grid

In this paper we describe developed hardware/software composition enabling field programmable logic devices (FPGAs) in the UNICORE grid environment. A Smith-Waterman algorithm for aminoacid and DNA sequences is implemented in FPGA PCI extension card, interfaced with Linux workstation running kernel 2.6 series through special low level module and is exposed to the final user with the UNICORE middleware. This approach gives remote, secure, uniform and easy to use graphical access to the very specialised hardware accelerated service. Additionally, great flexibility to the UNIX system is provided due to separation of a different hardware and software levels using UNICORE plugins. The approach is very effective in a BioGrid context, where we are interested in integration of sophisticate accelerated distributed computational resources and providing general grid functionality consisting of single login, job submission and control mechanism to the life sciences community
Preserved Dendritic Cell HLA-DR Expression and Reduced Regulatory T Cell Activation in Asymptomatic Plasmodium falciparum and P. vivax Infection

ABSTRACT Clinical illness with Plasmodium falciparum or Plasmodium vivax compromises the function of dendritic cells (DC) and expands regulatory T (Treg) cells. Individuals with asymptomatic parasitemia have clinical immunity, restricting parasite expansion and preventing clinical disease. The role of DC and Treg cells during asymptomatic Plasmodium infection is unclear. During a cross-sectional household survey in Papua, Indonesia, we examined the number and activation of blood plasmacytoid DC (pDC), CD141+, and CD1c+ myeloid DC (mDC) subsets and Treg cells using flow cytometry in 168 afebrile children (of whom 15 had P. falciparum and 36 had P. vivax infections) and 162 afebrile adults (of whom 20 had P. falciparum and 20 had P. vivax infections), alongside samples from 16 patients hospitalized with uncomplicated malaria. Unlike DC from malaria patients, DC from children and adults with asymptomatic, microscopy-positive P. vivax or P. falciparum infection increased or retained HLA-DR expression. Treg cells in asymptomatic adults and children exhibited reduced activation, suggesting increased immune responsiveness. The pDC and mDC subsets varied according to clinical immunity (asymptomatic or symptomatic Plasmodium infection) and, in asymptomatic infection, according to host age and parasite species. In conclusion, active control of asymptomatic infection was associated with and likely contingent upon functional DC and reduced Treg cell activation.
Analysis of Trafficking, Stability and Function of Human Connexin 26 Gap Junction Channels with Deafness-Causing Mutations in the Fourth Transmembrane Helix

Human Connexin26 gene mutations cause hearing loss. These hereditary mutations are the leading cause of childhood deafness worldwide. Mutations in gap junction proteins (connexins) can impair intercellular communication by eliminating protein synthesis, mis-trafficking, or inducing channels that fail to dock or have aberrant function. We previously identified a new class of mutants that form non-functional gap junction channels and hemichannels (connexons) by disrupting packing and inter-helix interactions. Here we analyzed fourteen point mutations in the fourth transmembrane helix of connexin26 (Cx26) that cause non-syndromic hearing loss. Eight mutations caused mis-trafficking (K188R, F191L, V198M, S199F, G200R, I203K, L205P, T208P). Of the remaining six that formed gap junctions in mammalian cells, M195T and A197S formed stable hemichannels after isolation with a baculovirus/Sf9 protein purification system, while C202F, I203T, L205V and N206S formed hemichannels with varying degrees of instability. The function of all six gap junction-forming mutants was further assessed through measurement of dye coupling in mammalian cells and junctional conductance in paired Xenopus oocytes. Dye coupling between cell pairs was reduced by varying degrees for all six mutants. In homotypic oocyte pairings, only A197S induced measurable conductance. In heterotypic pairings with wild-type Cx26, five of the six mutants formed functional gap junction channels, albeit with reduced efficiency. None of the mutants displayed significant alterations in sensitivity to transjunctional voltage or induced conductive hemichannels in single oocytes. Intra-hemichannel interactions between mutant and wild-type proteins were assessed in rescue experiments using baculovirus expression in Sf9 insect cells. Of the four unstable mutations (C202F, I203T, L205V, N206S) only C202F and N206S formed stable hemichannels when co-expressed with wild-type Cx26. Stable M195T hemichannels displayed an increased tendency to aggregate. Thus, mutations in TM4 cause a range of phenotypes of dysfunctional gap junction channels that are discussed within the context of the X-ray crystallographic structure.
Re-Constructing Historical Adélie Penguin Abundance Estimates by Retrospectively Accounting for Detection Bias

Seabirds and other land-breeding marine predators are considered to be useful and practical indicators of the state of marine ecosystems because of their dependence on marine prey and the accessibility of their populations at breeding colonies. Historical counts of breeding populations of these higher-order marine predators are one of few data sources available for inferring past change in marine ecosystems. However, historical abundance estimates derived from these population counts may be subject to unrecognised bias and uncertainty because of variable attendance of birds at breeding colonies and variable timing of past population surveys. We retrospectively accounted for detection bias in historical abundance estimates of the colonial, land-breeding Adélie penguin through an analysis of 222 historical abundance estimates from 81 breeding sites in east Antarctica. The published abundance estimates were de-constructed to retrieve the raw count data and then re-constructed by applying contemporary adjustment factors obtained from remotely operating time-lapse cameras. The re-construction process incorporated spatial and temporal variation in phenology and attendance by using data from cameras deployed at multiple sites over multiple years and propagating this uncertainty through to the final revised abundance estimates. Our re-constructed abundance estimates were consistently higher and more uncertain than published estimates. The re-constructed estimates alter the conclusions reached for some sites in east Antarctica in recent assessments of long-term Adélie penguin population change. Our approach is applicable to abundance data for a wide range of colonial, land-breeding marine species including other penguin species, flying seabirds and marine mammals.
Interactive region-based linear 3D face models

Linear models, particularly those based on principal component analysis (PCA), have been used successfully on a broad range of human face-related applications. Although PCA models achieve high compression, they have not been widely used for animation in a production environment because their bases lack a semantic interpretation. Their parameters are not an intuitive set for animators to work with. In this paper we present a linear face modelling approach that generalises to unseen data better than the traditional holistic approach while also allowing click-and-drag interaction for animation. Our model is composed of a collection of PCA sub-models that are independently trained but share boundaries. Boundary consistency and user-given constraints are enforced in a soft least mean squares sense to give flexibility to the model while maintaining coherence. Our results show that the region-based model generalises better than its holistic counterpart when describing previously unseen motion capture data from multiple subjects. The decomposition of the face into several regions, which we determine automatically from training data, gives the user localised manipulation control. This feature allows to use the model for face posing and animation in an intuitive style.
Predictors of Leadership Behavior in Early Career White-Collar Professionals

This research studies the leadership development of 243 working MBA students and finds personal characteristics and career context to be associated with development of leadership behavior in general, and with specific leadership styles. Specifically, personal characteristics of proactiveness, risk taking, and social skill, and the career contextual variables number of subordinates, level of interaction with others, and time pressure are found to be associated with increased development of leader behavior. Gender was not a differentiator in the development of leadership; however, socially skilled males with high interaction working under time pressure exhibit directive leadership, while personality variables are associated with egalitarian leadership.