Miracle-dz/UniFAR-FTUs huggingface dataset:
[Subcellular distribution of daunorubicin in the P-glycoprotein-mediated multidrug-resistant cell line K562/ADR].

OBJECTIVE To examine subcellular distribution of daunorubicin (DNR) in P-glycoprotein-mediated multidrug-resistant cell line K562/ADR and its relation to multidrug resistance. METHODS The subcellular distribution of DNR in K562/ADR was studied by confocal scanning laser microscopy (CSLM), fluorometry, RT-PCR. Rhodamine 123, NBD-ceramide and neutral red as fluorescent probes to stain the mitochondria, Golgi apparatus and lysosomes respectively were used to identify the subcellular compartments wherein DNR was sequestered. Effect of verapamil, chloroquine and brefeldin A on DNR distribution and accumulation was examined. RESULTS Compared with the drug-sensitive cell K562/S in which DNR fluorescence diffusely appeared in the nucleus and cytoplasm, DNR in K562/ADR cells was distributed to the perinuclear region and peripheral cytoplasm, It was scenty in the nucleus and other cytoplasmic regions, as suggested by the distribution of Rhodamine123. Only verapamil, but not chloroquine and brefeldin A, could markedly restore diffuse cytoplasmic and nuclear fluorescence distribution in the resistant cell line. CONCLUSION Altered subcellular distribution of DNR in drug resistant cell line may participate in the generation of multidrug resistance in which P-glycoprotein plays an important role.
Downregulation of intracellular nm23-H1 prevents cisplatin-induced DNA damage in oesophageal cancer cells: possible association with Na+, K+-ATPase

Previously, we showed that expression of nm23-H1 is associated inversely with sensitivity to cisplatin in human oesophageal squamous cell carcinoma (OSCC). The present study was undertaken to investigate the association of nm23-H1 expression with cisplatin-induced DNA damage in OSCC using antisense nm23-H1 transfectants. YES-2/AS-12, an antisense nm23-H1-transfected OSCC cell line, showed significantly reduced expression of intracellular nm23-H1 protein compared with that in parental YES-2 cells and YES-2/Neo transfectants. Surface expression of nm23-H1 protein was not observed in any of the three cell lines. PCR analysis for DNA damage demonstrated that YES-2/AS-12 cells were more resistant to nuclear and mitochondrial DNA damage by cisplatin than were YES-2/Neo cells. In addition, mitochondrial membrane potentials and DNA fragmentation assays confirmed that YES-2/AS-12 was more resistant than YES-2/Neo to apoptosis induced by cisplatin. In contrast, YES-2/AS-12 was more sensitive to ouabain, a selective inhibitor of Na+, K+-ATPase, than YES-2 and YES-2/Neo. Pre-treatment with ouabain resulted in no differences in cisplatin sensitivity between the three cell lines examined. Intracellular platinum level in YES-2/AS-12 was significantly lower than that in YES-2 and YES-2/Neo following incubation with cisplatin, whereas ouabain pre-treatment resulted in no differences in intracellular platinum accumulations between the three cell lines. Our data support the conclusion that reduced expression of intracellular nm23-H1 in OSCC cells is associated with cisplatin resistance via the prevention of both nuclear and mitochondrial DNA damage and suggest that it may be related to Na+, K+-ATPase activity, which is responsible for intracellular cisplatin accumulation. © 2000 Cancer Research Campaign
A Comparison of the Quality of Work Experience in Government and Private Organizations

A total of 5979 employees from a number of Australian government and private sector organizations participated in a survey measuring the quality of their work experience. This article examines the differences found between government and private sector employees' ratings of their work experience. The results of this study indicate that the government employees rate the quality of their work experience significantly below the level of their private sector counterparts. The most significant difference in the ratings was between high status government vs. high status private employees, with high status private employees rating their work experience well above the equivalent government group. An association was also found between the type of organization and employees' motivation for work. People working in public organizations were motivated more by factors such as providing a service or product that helps other people or self-development/self-fulfillment than private sector employees. Those in private organizations were motivated more by extrinsic factors such as money in order to obtain an enjoyable and pleasurable life. High status government employees were more intrinsically oriented than all other occupational groups. The lower rating of government employees' work experience may be related to the lack of fulfilling intrinsic factors in their work situations. The results of this study are similar to the results of previous studies which have compared work satisfaction in government vs. private organizations. The results suggest the need for well planned and coordinated organizational development programs in government departments.
After-hours general practice clinics are unlikely to reduce low acuity patient attendances to metropolitan Perth emergency departments.

OBJECTIVE To model the effectiveness of after-hours general practice (GP) in reducing metropolitan Perth emergency departments' (ED) low acuity patient (LAP) attendances and costs. METHODS We estimated LAP attendances by comparison of the product of (A) the difference between self-referred and GP-referred ED discharge rates and (B) total self-referred attendances (LAP attendances = A x B). We then compared after-hours ED LAP attendance rates and costs with inner metropolitan "working-week" ED LAP attendance rates and costs, when GP services are maximally available. RESULTS Working-week LAP attendances comprised 8.2% (95% CI, 8.0%-8.4%) of inner metropolitan ED attendances. Excess weekend and evening LAP attendances were estimated to comprise 16.5% (95%CI, 15.9%-17.0%) and 4.5% (95%CI, 4.1%-4.9%) of outer and inner metropolitan ED attendances respectively and totalled less than 3.0% of ED costs. CONCLUSIONS Low acuity patients form a relatively constant, inexpensive proportion of ED workloads. After-hours GP LAP services are unlikely to significantly reduce ED attendances or costs.
The interplay of self-interest and equity in coalition formation

In this paper we investigated the interplay of self-interest and equity concerns in coalition formation by manipulating the number of units in which the coalition payoff is made available, and by manipulating the way people are allowed to interact. Results of three experiments showed that when the coalition payoff was such that members of each possible coalition could obtain an equitable payoff share, the outcome tended to be coalitions that also maximized the payoff of its members. However, when the payoff was such that people had to make trade-offs between maximizing their payoff share and obtaining an equitable payoff share, it took longer to form a coalition and it was harder to maintain a coalition. Moreover, depending on the way people were allowed to interact, the final outcome was a coalition that maximized the payoff of its members or a coalition that provided them with an equitable payoff share. Copyright © 2004 John Wiley & Sons, Ltd.
Enabling Localized Peer-to-Peer Electricity Trading Among Plug-in Hybrid Electric Vehicles Using Consortium Blockchains

We propose a localized peer-to-peer (P2P) electricity trading model for locally buying and selling electricity among plug-in hybrid electric vehicles (PHEVs) in smart grids. Unlike traditional schemes, which transport electricity over long distances and through complex electricity transportation meshes, our proposed model achieves demand response by providing incentives to discharging PHEVs to balance local electricity demand out of their own self-interests. However, since transaction security and privacy protection issues present serious challenges, we explore a promising consortium blockchain technology to improve transaction security without reliance on a trusted third party. A localized P2P Electricity Trading system with COnsortium blockchaiN (PETCON) method is proposed to illustrate detailed operations of localized P2P electricity trading. Moreover, the electricity pricing and the amount of traded electricity among PHEVs are solved by an iterative double auction mechanism to maximize social welfare in this electricity trading. Security analysis shows that our proposed PETCON improves transaction security and privacy protection. Numerical results based on a real map of Texas indicate that the double auction mechanism can achieve social welfare maximization while protecting privacy of the PHEVs.
Knowledge, attitudes and practices vis-a-vis cervical cancer among registered nurses at the Faculty of Medicine, Khon Kaen University, Thailand.

BACKGROUND Cervical cancer is the most common cause of death from cancer among women in Thailand and in almost all patients, human papillomavirus (HPV) has been found. Various international studies indicate that the knowledge level vis-à-vis cervical cancer and HPV in the general population as well as healthcare professionals is low, but no such study has yet been done in Thailand. OBJECTIVES Our study's aim was to ascertain the level of knowledge, the attitudes and practices regarding cervical cancer among registered nurses working in Srinagarind (university) Hospital in an urban setting in Northeast Thailand. MATERIALS AND METHODS Systematic sampling was used and self-administered questionnaires were sent to 149 registered nurses; 133 (89.3%) of whom responded. Data were processed using descriptive statistics including frequency, percentages and the 95% CIs. RESULTS The respondents' averaged 34.6 years of age (median, 33; range, 21-56) while 54.6% had sexual partners and 61.4% had had normal deliveries. The respective median knowledge score, interquartile range and mean knowledge score and range for cervical cancer vs. HPV vs. cervical cancer prevention were: 11.00, 5.0, 10.14 (95%CI 9.6, 10.7), 0-15 vs. 4.00, 2.0, 3.58 (95%CI 3.5-4.2), 0-7 vs. 8, 3.00, 7.5 (95%CI 7.1-7.9), 1-11. 66.2% would like to have prophylactic HPV vaccines because they thought that it would prevent HPV infection (77.3%) or prevent cervical cancer (39.1%), which are major misunderstandings. CONCLUSION Almost all of the registered nurses working at Srinagarind Hospital have a moderate level of knowledge regarding cervical cancer and HPV but there are still some major misunderstandings; thus, educational pamphlets, notices and hospital announcements would be useful in increasing their knowledge.
Combining Deduction Modulo and Logics of Fixed-Point Definitions

Inductive and coinductive specifications are widely used in formalizing computational systems. Such specifications have a natural rendition in logics that support fixed-point definitions. Another useful formalization device is that of recursive specifications. These specifications are not directly complemented by fixed-point reasoning techniques and, correspondingly, do not have to satisfy strong monotonicity restrictions. We show how to incorporate a rewriting capability into logics of fixed-point definitions towards additionally supporting recursive specifications. Specifically, we describe a natural deduction calculus that adds a form of "closed-world'' equality - a key ingredient to supporting fixed-point definitions - to deduction modulo, a framework for extending a logic with a rewriting layer operating on formulas. We show that our calculus enjoys strong normalizability when the rewrite system satisfies general properties and we demonstrate its usefulness in specifying and reasoning about syntax-based descriptions. Our integration of closed-world equality into deduction modulo is based on an elimination principle for this form of equality that, for the first time, allows us to require finiteness of proofs without sacrificing stability under reduction.
Socialization of Student Teachers

In this study of organizational socialization, student teachers became more controlling in their perspectives as they completed their practice teaching. They became significantly more custodial in pupil-control orientation as well as more controlling in their orientation toward social problem solving. Student teachers also became less confident that they could overcome the limitations of home environment and family background. It is surprising that these same student teachers did not temper their optimistic belief that they had the personal ability to motivate and be effective with difficult students; in fact, their sense of personal teaching efficacy improved as their sense of general teaching efficacy declined. Similar students who were preparing to teach but had not yet enrolled in student teaching did not experience these changes in perspectives during the same time.
Rapid, Nongenomic Responses to Ecdysteroids and Catecholamines Mediated by a Novel Drosophila G-Protein-Coupled Receptor

Nongenomic response pathways mediate many of the rapid actions of steroid hormones, but the mechanisms underlying such responses remain controversial. In some cases, cell-surface expression of classical nuclear steroid receptors has been suggested to mediate these effects, but, in a few instances, specific G-protein-coupled receptors (GPCRs) have been reported to be responsible. Here, we describe the activation of a novel, neuronally expressed Drosophila GPCR by the insect ecdysteroids ecdysone (E) and 20-hydroxyecdysone (20E). This is the first report of an identified insect GPCR interacting with steroids. The Drosophila melanogaster dopamine/ecdysteroid receptor (DmDopEcR) shows sequence homology with vertebrate β-adrenergic receptors and is activated by dopamine (DA) to increase cAMP levels and to activate the phosphoinositide 3-kinase pathway. Conversely, E and 20E show high affinity for the receptor in binding studies and can inhibit the effects of DA, as well as coupling the receptor to a rapid activation of the mitogen-activated protein kinase pathway. The receptor may thus represent the Drosophila homolog of the vertebrate “γ-adrenergic receptors,” which are responsible for the modulation of various activities in brain, blood vessels, and pancreas. Thus, DmDopEcR can function as a cell-surface GPCR that may be responsible for some of the rapid, nongenomic actions of ecdysteroids, during both development and signaling in the mature adult nervous system.
E-parliament services as tools for anti-corruption and transparency

Literature is rife on the dissatisfaction of the Nigerian public on the poor performance of political institutions such as the National Assembly of Nigeria (NASS). However, e-parliaments mostly in countries of America and Europe show legislators have considerably used Information and Communication Technology (ICT) to form new relationships and engagement of the public to participate in the performance of legislative functions, fight corruption, and strengthen representative democracy. However, this does not appear to be the case in the NASS. This study explores the main factors of e-parliament adoption for anti-corruption and finds out the perceptions and attitude of legislators and citizens of the phenomenon. The theoretical lens is a modified Unified Theory of Acceptance and Use of Technology (UTAUT) by
The relationship between the work unit’s conflict management styles and bullying at work: Moderation by conflict frequency

This study investigates the relationship between the work unit’s conflict management styles and bullying at work. Inspired by the Dual Concern framework, the authors assume a positive relationship between avoiding, forcing and yielding and bullying and a negative relationship between problem-solving and bullying. Moreover, they expected these relationships to be intensified by conflict frequency. The results (N = 5062) reveal an unsatisfactory fit when not taking into account conflict frequency as a moderator. A multigroup SEM accounting for the moderating role of conflict frequency (five groups) does yield an acceptable fit: the ‘very rarely’ and ‘yearly’ group, and the ‘monthly’ and ‘weekly’ group show equal path coefficients. The ‘daily’ conflict group has its own parameters. Forcing and avoiding associate positively and problem-solving associates negatively with bullying; and these relationships intensify as a function of conflict frequency. There is no relationship between yielding and bullying at work within the various conflict frequency groups.
Has Racism Declined in America?

In recent years as public opinion polls have shown a decline in racist responses, white Americans have strongly resisted school desegregation and affirmative action programs. Hence, there has been a debate over the extent to which racism has really declined. The theory of modern racism addresses these issues, distinguishing between old-fashioned racial beliefs recognized by everyone as racism and a new set of beliefs arising from the conflicts of the civil rights movement. The theory proposes that antiblack feeling remains high and has been displaced from the socially undesirable old-fashioned beliefs onto the new beliefs where the racism is not recognized. Three experiments were performed; results showed that, regardless of context, the old-fashioned items were perceived as more likely to reveal prejudice. The results are discussed in terms of their significance for opinion polling and continuing racial conflict in America.
Mexican Parenting Questionnaire (MPQ)

The present study was conducted in four phases and constructed a self-report parenting instrument for use with Mexican immigrant mothers of children aged 6 to 10. The 14-item measure was based on semistructured qualitative interviews with Mexican immigrant mothers (N = 10), was refined by a focus group of Mexican immigrant mothers (N = 5), and was then completed by a larger sample of Mexican immigrant mothers (N = 168). Children’s socioemotional and behavioral competence was examined on a subset of children (N = 30) from family drawings and teacher reports of behavior. Items of the parenting instrument pertained to one of three general parenting constructs: Warmth, Monitoring, and Discipline. Using confirmatory factor analyses (CFA) and respecification procedures, the three models were found to be a good fit with the data. Subscales consisted of moderate levels of internal consistency and predicted several children’s behaviors.
Evidence for Cognitive Load Theory

Dixon (1991) and Goldman (1991) have provided thoughtful commentaries on Chandler and Sweller (1991). The general issue they raise concerns the scientific procedures we should use when conducting research in cognition and instruction. It is an issue of great importance, and we welcome the opportunity provided by their criticisms to discuss the techniques we use. To clarify the findings based on cognitive load theory, we begin by indicating the essential characteristics of a theory concerned with cognition and instruction, followed by a brief history of cognitive load theory to make clear that it has these characteristics. These statements will then be used to address specific comments made by Goldman and Dixon.
Physician judgement is a crucial adjunct to pneumonia severity scores in low-risk patients

This study investigates the reasons for hospitalisation in patients with low-risk (CURB-65 score 0–1) community-acquired pneumonia (CAP), with a view to identifying the potential for improving outpatient management. As part of a prospective observational study of CAP, we evaluated reasons for hospitalisation in these low-risk patients. 565 patients had low-risk CAP and 420 of these were admitted (for >12 h). 39.3% had additional markers of severity justifying admission, 29.5% of the admissions were required for further management that could not be provided rapidly in the community, 11.9% had unsafe social circumstances and 19.3% had no clinical reason justifying hospitalisation. 30-day mortality was increased in patients with additional severity markers (6.7%), which was significantly higher compared with 0% for patients awaiting investigations (p=0.009) and 0% without a clear indication for hospitalisation (p=0.04). In a logistic regression analysis, parameters associated with 30-day mortality were chronic cardiac comorbidity (adjusted odds ratio (aOR) 5.73, 95% CI 1.52–21.6; p=0.01), acidosis (aOR 5.14, 95% CI 1.44–18.3; p=0.01), hypoxia (aOR 9.86, 95% CI 2.39–40.7; p=0.002) and multilobar chest radiograph shadowing (aOR 4.54, 95% CI 1.21–17.1; p=0.03). This study supports recommendations from international guidelines that pneumonia severity scores should be used as an adjunct to clinical judgement, when deciding on hospitalisation.
Rad51 supports triple negative breast cancer metastasis

In contrast to extensive studies on familial breast cancer, it is currently unclear whether defects in DNA double strand break (DSB) repair genes play a role in sporadic breast cancer development and progression. We performed analysis of immunohistochemistry in an independent cohort of 235 were sporadic breast tumours. This analysis suggested that RAD51 expression is increased during breast cancer progression and metastasis and an oncogenic role for RAD51 when deregulated. Subsequent knockdown of RAD51 repressed cancer cell migration in vitro and reduced primary tumor growth in a syngeneic mouse model in vivo. Loss of RAD51 also inhibited associated metastasis not only in syngeneic mice but human xenografts and changed the metastatic gene expression profile of cancer cells, consistent with inhibition of distant metastasis. This demonstrates for the first time a new function of RAD51 that may underlie the proclivity of patients with RAD51 overexpression to develop distant metastasis. RAD51 is a potential biomarker and attractive drug target for metastatic triple negative breast cancer, with the capability to extend the survival of patients, which is less than 6 months.
Cenozoic exhumation of the northern Sierra Nevada, California, from (U-Th)/He thermochronology

Apatite and zircon (U-Th)/He ages from a 100-km-long range-perpendicular transect in the northern Sierra Nevada, California, are used to constrain the exhumation history of the range since ca. 90 Ma. (U-Th)/He ages in apatite decrease from 80 Ma along the low western range flanks to 46 Ma in the higher elevations to the east. (U-Th)/He ages in zircon also show a weak inverse correlation with elevation, decreasing from 91 Ma in the west to 66 Ma in the east. Rocks near the range crest, sampled at elevations of 2200–2500 m, yield the youngest apatite helium ages (46–55 Ma), whereas zircon helium ages are more uniform across the divide. These data reveal relatively rapid cooling rates between ca. 90 and 60 Ma, which are consistent with relatively rapid exhumation rates of 0.2–0.8 km/m.y. , followed by a long period of slower exhumation (0.02–0.04 km/m.y.) from the early Paleogene to today. This is reflected in the low-relief morphology of the northern Sierra Nevada, where an Eocene erosional surface has long been identified. A long period of slow exhumation is also consistent with well-documented, widespread lateritic paleosols at the base of Eocene depositional units. Laterites preserved in the northern Sierra Nevada are the product of intense weathering in a subtropical environment and suggest an enduring, soil-mantled topography. We interpret this exhumation history as recording a Late Cretaceous to early Cenozoic period of relatively rapid uplift and unroofing followed by tectonic quiescence and erosional smoothing of Sierran topography through the Neogene. Well-documented recent incision appears to have had little effect on (U-Th)/He ages, suggesting that less than ∼3 km has been eroded from the Sierra Nevada since the early Cenozoic.