Miracle-dz/UniFAR-FTUs huggingface dataset:
Blue Stragglers, Young White Dwarfs, and UV-Excess Stars in the Core of 47 Tucanae

We used a set of archived Hubble Space Telescope/WFPC2 images to probe the stellar population in the core of the nearby galactic globular cluster (GGC) 47 Tuc. From the ultraviolet (UV) color magnitude diagrams (CMDs) obtained for ~4000 stars detected within the Planetary Camera (PC) field of view we have pinpointed a number of interesting objects: (1) 43 blue stragglers stars (BSSs), including 20 new candidates; (2) 12 bright (young) cooling white dwarfs (WDs) at the extreme blue region of the UV-CMD; and (3) a large population of UV-excess (UVE) stars, lying between the BSS and the WD sequences. The colors of the WD candidates identified here define a clean pattern in the CMDs, which define the WD cooling sequence. Moreover, both the location on the UV-CMDs and the number of WDs are in excellent agreement with the theoretical expectations. The UVE stars discovered here represent the largest population of anomalous blue objects ever observed in a globular cluster—if the existence of such a large population is confirmed, we have finally found the long-searched-for population of interacting binaries predicted by the theory. Finally, we have investigated the feasibility of the optical identification of the companions of the binary X-ray sources recently detected by Chandra and of binary millisecond pulsars (MSPs) residing in the core of 47 Tuc. Unfortunately, the extreme faintness expected for the MSP companions, together with the huge stellar crowding in the cluster center, prevents statistically reliable identifications based only on positional coincidences.
Early Genital Naming

To evaluate the clinical impression that young girls are given little or confusing information about their genitals, a sample of 117 mothers with 1− to 4-year-old children were asked which words for genitals, if any, they used with their children. The ethnically and socioeconomically heterogeneous sample was composed of 63 girls and 54 boys, with the average ages of 26 and 29 months, respectively. Neither boys nor girls were likely to be given a standard anatomical genital term, although many children received colorful colloquial expressions. However, girls were less likely than boys to receive a term for their genitals. Receiving names for genitals was related to certain family circumstances, such as higher parental education, exposure to adult male nudity, having a sibling of the opposite sex, and cosleeping. Pediatric health professionals have the opportunity to contribute to early sex education by conveying accurate information regarding genital terms in the course of routine physical examinations.
Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis

The involvement of two R2R3-MYB genes from Pinus taeda L. , PtMYB1 and PtMYB8, in phenylpropanoid metabolism and secondary cell wall biogenesis was investigated in planta. These pine MYBs were constitutively overexpressed (OE) in Picea glauca (Moench) Voss, used as a heterologous conifer expression system. Morphological, histological, chemical (lignin and soluble phenols), and transcriptional analyses, i.e. microarray and reverse transcription quantitative PCR (RT-qPCR) were used for extensive phenotyping of MYB-overexpressing spruce plantlets. Upon germination of somatic embryos, root growth was reduced in both transgenics. Enhanced lignin deposition was also a common feature but ectopic secondary cell wall deposition was more strongly associated with PtMYB8-OE. Microarray and RT-qPCR data showed that overexpression of each MYB led to an overlapping up-regulation of many genes encoding phenylpropanoid enzymes involved in lignin monomer synthesis, while misregulation of several cell wall-related genes and other MYB transcription factors was specifically associated with PtMYB8-OE. Together, the results suggest that MYB1 and MYB8 may be part of a conserved transcriptional network involved in secondary cell wall deposition in conifers.
Co-occurrence of ADHD and autism spectrum disorders: phenomenology and treatment

The Diagnostic and Statistical Manual of Mental Disorders (4th Edition) prohibits the co-diagnosis of attention-deficit/hyperactivity disorder (ADHD) and an autism spectrum disorder (ASD). However, recent studies indicate that co-occurrence of clinically significant ADHD and autistic symptoms is common, and that some genes may influence both disorders. Children with the combination of ADHD and motor coordination problems are particularly likely to have an ASD. These co-occurrences of symptoms are important since children with ASD in addition to ADHD symptoms may respond poorly to standard ADHD treatments or have increased side effects. Such children may benefit from additional classes of pharmacologic agents (i.e., α-agonists, selective serotonin reuptake inhibitors and neuroleptics). They may also benefit from social skills therapy, individual and family psychotherapy, behavioral therapy and other nonpharmacologic interventions.
Cytological Visualization of DNA Transposons and Their Transposition Pattern in Somatic Cells of Maize

Global genomic analysis of transposable element distributions of both natural (En/Spm, Ac–Ds, and MuDR/Mu) and modified (RescueMu) types was performed by fluorescence in situ hybridization (FISH) on somatic chromosomes coupled with karyotyping of each chromosome. In lines without an active transposable element, the locations of silent En/Spm, Ac–Ds, and MuDR/Mu elements were visualized, revealing variation in copy number and position among lines but no apparent locational bias. The ability to detect single elements was validated by using previously mapped active Ac elements. Somatic transpositions were documented in plants containing an engineered Mutator element, RescueMu, via use of the karyotyping system. By analyzing the RescueMu lines, we found that transposition of RescueMu in root-tip cells follows the cut-and-paste type of transposition. This work demonstrates the utility of FISH and karyotyping in the study of transposon activity and its consequences.
Gating of Na channels. Inactivation modifiers discriminate among models

Macroscopic Na currents were recorded from N18 neuroblastoma cells by the whole-cell voltage-clamp technique. Inactivation of the Na currents was removed by intracellular application of proteolytic enzymes, trypsin, alpha-chymotrypsin, papain, or ficin, or bath application of N- bromoacetamide. Unlike what has been reported in squid giant axons and frog skeletal muscle fibers, these treatments often increased Na currents at all test pulse potentials. In addition, removal of inactivation gating shifted the midpoint of the peak Na conductance- voltage curve in the negative direction by 26 mV on average and greatly prolonged the rising phase of Na currents for small depolarizations. Polypeptide toxins from Leiurus quinquestriatus scorpion and Goniopora coral, which slow inactivation in adult nerve and muscle cells, also increase the peak Na conductance and shift the peak conductance curve in the negative direction by 7-10 mV in neuroblastoma cells. Control experiments argue against ascribing the shifts to series resistance artifacts or to spontaneous changes of the voltage dependence of Na channel kinetics. The negative shift of the peak conductance curve, the increase of peak Na currents, and the prolongation of the rise at small depolarization after removal of inactivation are consistent with gating kinetic models for neuroblastoma cell Na channels, where inactivation follows nearly irreversible activation with a relatively high, voltage- independent rate constant and Na channels open only once in a depolarization. As the same kind of experiment does not give apparent shifting of activation and prolongation of the rising phase of Na currents in adult axon and muscle membranes, the Na channels of these other membranes probably open more than once in a depolarization.
New developments in ordered mesoporous materials for drug delivery

Silica-based ordered mesoporous materials have experienced impressive consideration in drug delivery applications since they were first employed as drug delivery systems back in 2001. The possibility of tuning the physicochemical properties of the matrices together with the development of stimuli-responsive technologies are two of the main reasons for the great scientific attention on these bioceramics as conventional drug delivery systems. However, the properties of ordered mesoporous silicas have fuelled the possibility of covalently grafting certain biomolecules to stimulate selected target cells able to create new tissue, powering their employment in tissue engineering technologies, especially for bone tissue regeneration.
Disruption of Right Prefrontal Cortex by Low-Frequency Repetitive Transcranial Magnetic Stimulation Induces Risk-Taking Behavior

Decisions require careful weighing of the risks and benefits associated with a choice. Some people need to be offered large rewards to balance even minimal risks, whereas others take great risks in the hope for an only minimal benefit. We show here that risk-taking is a modifiable behavior that depends on right hemisphere prefrontal activity. We used low-frequency, repetitive transcranial magnetic stimulation to transiently disrupt left or right dorsolateral prefrontal cortex (DLPFC) function before applying a well known gambling paradigm that provides a measure of decision-making under risk. Individuals displayed significantly riskier decision-making after disruption of the right, but not the left, DLPFC. Our findings suggest that the right DLPFC plays a crucial role in the suppression of superficially seductive options. This confirms the asymmetric role of the prefrontal cortex in decision-making and reveals that this fundamental human capacity can be manipulated in normal subjects through cortical stimulation. The ability to modify risk-taking behavior may be translated into therapeutic interventions for disorders such as drug abuse or pathological gambling.
Analysis of setup error based on CTVision for nasopharyngeal carcinoma during IGRT

The aim of the present study was to investigate the role of CTVision in interfractional setup errors during intensity‐modulated radiation therapy (IMRT) in 12 nasopharyngeal carcinoma (NPC) patients. The trend of setup errors as a function of time during a fractionated radiotherapy course was investigated, and the influence of reconstructive thickness on image reconstruction for setup errors was analyzed. The appropriate planning target volume (PTV) margin and planning risk volume (PRV) margin were defined to provide a reference for the design of IMRT for NPC. Based on CTVision, online CT was performed weekly for each patient. Setup errors were measured by registration between the CT reconstructed image and reference image. Mean of setup errors, estimated population systematic (:), and population random (σ) errors were calculated using SPSS (v15.0). Optimum PTV and PRV margins were calculated. In the clinical data, for the LR (left–right), SI (superior–inferior), and AP (anterior–posterior) directions, : was 0.8, 0.8, and 1.0 mm, respectively, and σ was 1.0, 1.3, and 0.8 mm, respectively. In the LR, SI, and AP directions, PTV margins were at least 2.7, 2.9, and 3.0 mm, respectively, and PRV margins were at least 1.5, 1.7, and 1.7 mm, respectively. No significant differences in setup errors were observed during the fractionated radiotherapy course (p>0.05). However, CT image reconstruction with different thicknesses affected the accuracy of measurements for setup errors, particularly in the SI direction. The application of CTVision to correct setup errors is important and can provide reasonable margins to guarantee the coverage of PTVs and spare organs at risk. A thickness of 3 mm in the reconstructed image is appropriate for the measurement of setup errors by image registration. PACS number(s): 87.55. Qr
Diversity of Fungal Species Associated with Peanuts in Storage and the Levels of Aflatoxins in Infected Samples

The threat of aflatoxin contamination in food commodities and its association with health risks in both animals and humans continues to raise increasing concern over years. In this report, fungal species found in association with peanuts in storage and their potential to produce aflatoxin in collected samples was determined. About 60 to 70% of collected peanut samples were infected with various moulds including Rhizopus stolonifer, Fusarium sp. , Aspergilus flavus, other Aspergillus sp. , Penicillium sp. , Eurotium repens, Sclerotium sp. , Rhizoctonia sp. , and Aspergillus Parasiticus. Eurotium repens, Aspergillus Parasiticus, and A. flavus were found to be the most patent aflatoxigenic strains. The average levels of aflatoxins detected in the seed samples were far above 100 ppb. This level of toxicity is more than five times higher than the acceptable dosage (20 ppb: US Standards) in edible peanuts. This report points out the health risks associated with aflatoxin contamination in edible food commodities despite enormous efforts to control this mycotoxin. Current research efforts to control or minimize the intake of aflatoxins especially in warmer regions of the world are hereby included.
Detection of Radial Surface Brightness Fluctuations and Color Gradients in Elliptical Galaxies with the Advanced Camera for Surveys

We study surface brightness fluctuations (SBFs) in a sample of eight elliptical galaxies using the Advanced Camera for Surveys (ACS) Wide Field Channel (WFC) data drawn from the Hubble Space Telescope archive. SBF magnitudes in the F814W bandpass and galaxy colors from F814W, F435W, and F606W images, when available, are presented. Galaxy surface brightness profiles are determined as well. We present the first SBF-broadband color calibration for the ACS WFC F814W bandpass and (relative) distance moduli estimates for seven of our galaxies. We detect and study in detail the SBF variations within individual galaxies as a probe of possible changes in the underlying stellar populations. Inspecting both the SBF and color gradients in comparison to model predictions, we argue that SBFs and SBF gradients can in principle be used for unraveling the different evolutionary paths taken by galaxies, although a more comprehensive study of this issue would be required. We confirm that the radial variation of galaxy stellar population properties is mainly connected to the presence of radial chemical abundance gradients, with the outer galaxy regions being more metal-poor than the inner ones.
Interaction of a Cannabinoid-2 Agonist With Tramadol on Nociceptive Thresholds and Immune Responses in a Rat Model of Incisional Pain

The aim of this study was to elucidate the antinociceptive interaction between cannabinoids and tramadol and their impact on proinflammatory response, in terms of serum intereleukin-6 (IL-6) and interleukin-2 (IL-2) release, in a rat model of incisional pain. Prospective randomized trial assessing the individual or combined application of intraperitoneal tramadol (10 mg/kg) and the selective cannabinoid-2 (CB-2) agonist (R,S)-AM1241 (1 mg/kg) applied postsurgical stress stimulus. Pharmacological specificity was established by antagonizing tramadol with naloxone (0.3 mg/kg) and (R,S)-AM1241 with SR144528 (1 mg/kg). Thermal allodynia was assessed by hot plate test 30 (T30), 60 (T60), and 120 (T120) minutes after incision. Blood samples for plasma IL-6 and IL-2 level determination were obtained 2 hours after incision. Data from 42 rats were included in the final analyses. Significant augmentation of thermal threshold was observed at all time points, after administration of either tramadol or (R,S)-AM1241 compared with the control group (P = 0.004 and P = 0.015, respectively). The combination of (R,S)-AM1241 plus tramadol promoted the induced antinociception in an important manner compared with control (P = 0.002) and (R,S)-AM1241 (P = 0.022) groups. Although the antiallodynic effect produced by tramadol was partially reversed by naloxone 30 and 60 minutes after incision (P = 0.028 and P = 0.016, respectively), SR144528 blocked the effects of (R,S)-AM1241 administration in a significant manner (P = 0.001) at all time points. Similarly, naloxone plus SR144528 also blocked the effects of the combination of (R,S)-AM1241 with tramadol at all time points (P = 0.000). IL-6 level in (R,S)-AM1241 plus tramadol group was significantly attenuated compared with control group (P = 0.000). Nevertheless, IL-2 levels remained unchanged in all experimental groups. It seems that the concomitant administration of a selective CB-2 agonist with tramadol in incisional pain model may improve antinociceptive effects and immune responses of cannabinoids, but this effect does not seem to be superior to that of tramadol alone.
Preparation of Heteroenzyme Conjugates: Trypsin‐Chymotrypsin and Trypsin‐Alkaline Phosphatase

Hybrid enzymes which have two different enzyme activities linked together covalently may be useful reagents for various applications, such as the determination of complex biological structures. The present paper describes the preparation and purification of two such enzyme‐enzyme conjugates, namely, trypsin‐chymotrypsin and trypsin‐alkaline phosphatase. Whereas the former has been prepared by using the well‐known bifunctional reagent glutaraldehyde, the latter exploited the Schiff base formation between the oxidized carbohydrate moiety of alkaline phosphatase and the free amino groups of trypsin.
Measurements and Analysis of Polycyclic Aromatic Hydrocarbons near a Major Interstate

Polycyclic aromatic hydrocarbons (PAHs) were measured near Interstate 40, just east of Research Triangle Park, North Carolina, USA. The goals of this project were to ascertain whether a sufficient quantity of PAHs could be collected using low flow (16.7 L/minute) over 8-h periods and if so, do investigate how the PAHs correlate to local sources, atmospheric pollutants and meteorology. The 8-h integrated samples were collected on 20 sampling days over a two month period during fall 2014. The samples were collected using low flow (BGI Incorporated PQ200) fine particulate samplers analyzed using gas chromatography-mass spectrometry (GC-MS). Temporal distributions of the PAHs (average mean 9.2 nanogram/cubic meter ±9.0 std) were compared to traffic count, and meteorological and pollutant data collected at the near roadway station. Using the meteorological data (i.e., wind speed and direction vector data), wind roses were created illustrating the local sources of the PAHs. In terms of correlation to atmospheric oxidants, (i.e., ozone, nitrogen dioxide and nitric oxide) wind rose analysis illustrated the morning hours which were predominantly southern winds, while the afternoon hours illustrated southerly and easterly winds, which suggests that the automobile traffic is the main source of PAHs. The nighttime hours wind rose shows winds from the northerly and easterly direction, which are predominantly from the RDU International Airport. Since the wind direction vectors illustrated that the afternoon hours (i.e., 12 p.m. to 8 p.m.) were from the interstate, comparisons were performed on the samples collected in this time period for both the traffic and pollutant data. The comparison of the traffic data showed a correlation with the number of vehicles (>60 feet i.e., heavy duty diesel engine vehicles). In addition, with the ozone, nitrogen dioxide and nitric oxide) there is a significant linear correlation between the sum of the measured PAHs with nitric oxide (NO), nitrogen dioxide (NO2) and ozone (O3) with the R2 values being 0.1, 0.04 and 0.07 respectively. An analysis of variance (ANOVA) statistical regression was performed on the pollutant data versus the measured sum of the PAHs. With the alpha set at 0.05, (α = 0.05) the p-values for O3, NO2 and NO were 0.00613, 0.000496 and 0.000264, respectively, which are significant. In addition, the PAH concentration found in this study compare favorably to other published studies (0.1 to 193.6 ng/m3) both nationally and internationally.
Extragalactic neutrino background from very young pulsars surrounded by supernova envelopes

We estimate the extragalactic muon neutrino background which is produced by hadrons injected by very young pulsars at an early phase after supernova explosion. It is assumed that hadrons are accelerated in the pulsar wind zone which is lled with thermal photons captured below the expanding supernova envelope. In collisions with those thermal photons hadrons produce pions which decay into muon neutrinos. At a later time, muon neutrinos are also produced by the hadrons in collisions with matter of the expanding envelope. We show that extragalactic neutrino background predicted by such a model should be detectable by the planned 1 km 2 neutrino detector if a signicant part of pulsars is born with periods shorter than10 ms. Since such population of pulsars is postulated by the recent models of production of extremely high energy cosmic rays, detection of neutrinos with predicted fluxes can be used as their observational test.
Dissecting a Hidden Gene Duplication: The Arabidopsis thaliana SEC10 Locus

Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst tethering complex, we examined whether this could be explained by a hidden gene duplication. Re-sequencing and manual assembly of the Arabidopsis thaliana SEC10 (At5g12370) locus revealed that this locus, comprising a single gene in the reference genome assembly, indeed contains two paralogous genes in tandem, SEC10a and SEC10b, and that a sequence segment of 7 kb in length is missing from the reference genome sequence. Differences between the two paralogs are concentrated in non-coding regions, while the predicted protein sequences exhibit 99% identity, differing only by substitution of five amino acid residues and an indel of four residues. Both SEC10 genes are expressed, although varying transcript levels suggest differential regulation. Homozygous T-DNA insertion mutants in either paralog exhibit a wild-type phenotype, consistent with proposed extensive functional redundancy of the two genes. By these observations we demonstrate that recently duplicated genes may remain hidden even in well-characterized genomes, such as that of A. thaliana. Moreover, we show that the use of the existing A. thaliana reference genome sequence as a guide for sequence assembly of new Arabidopsis accessions or related species has at least in some cases led to error propagation.
Using the Hough transform for HOLZ line identification in convergent beam electron diffraction

The Hough transform as a means for automatic line detection is applied for higher order Laue zone (HOLZ) line identification in convergent beam electron diffraction. The HOLZ line positions are commonly used to measure the strain state in the investigated material. Besides the automation, a very important aspect is the accuracy of the detected line positions. The limits are determined by the amount of noise in the pattern relative to the contrast of the HOLZ lines and the line width. Our investigations show that sub‐pixel resolution can be achieved routinely. We have also developed a new strain analysis procedure in which the dynamical shift of each individual line can be taken into account using a model based on several Ewald spheres with different radii. In comparison to the effective high voltage method, finer details of the dispersion surface can be considered, which increases the accuracy of the strain analysis. A measurement of the thermal expansion of aluminium is presented as a model experiment. The lattice constants were determined with an accuracy of about 10−4.