Miracle-dz/UniFAR-FTUs huggingface dataset:
Derricin and Derricidin Inhibit Wnt/β-Catenin Signaling and Suppress Colon Cancer Cell Growth In Vitro

Overactivation of the Wnt/β-catenin pathway in adult tissues has been implicated in many diseases, such as colorectal cancer. Finding chemical substances that can prevent this phenomenon is an emerging problem. Recently, several natural compounds have been described as Wnt/β-catenin inhibitors and might be promising agents for the control of carcinogenesis. Here, we describe two natural substances, derricin and derricidin, belonging to the chalcone subclass, that show potent transcriptional inhibition of the Wnt/β-catenin pathway. Both chalcones are able to affect the cell distribution of β-catenin, and inhibit Wnt-specific reporter activity in HCT116 cells and in Xenopus embryos. Derricin and derricidin also strongly inhibited canonical Wnt activity in vitro, and rescued the Wnt-induced double axis phenotype in Xenopus embryos. As a consequence of Wnt/β-catenin inhibition, derricin and derricidin treatments reduce cell viability and lead to cell cycle arrest in colorectal cancer cell lines. Taken together, our results strongly support these chalcones as novel negative modulators of the Wnt/β-catenin pathway and colon cancer cell growth in vitro.
A kuratowski-type theorem for planarity of partially embedded graphs

A partially embedded graph (or PEG) is a triple (G,H,EH), where G is a graph, H is a subgraph of G, and EH is a planar embedding of H. We say that a PEG (G,H,EH) is planar if the graph G has a planar embedding that extends the embedding EH. We introduce a containment relation of PEGs analogous to graph minor containment, and characterize the minimal non-planar PEGs with respect to this relation. We show that all the minimal non-planar PEGs except for finitely many belong to a single easily recognizable and explicitly described infinite family. We also describe a more complicated containment relation which only has a finite number of minimal non-planar PEGs. Furthermore, by extending an existing planarity test for PEGs, we obtain a polynomial-time algorithm which, for a given PEG, either produces a planar embedding or identifies a minimal obstruction.
GUIDING COMPUTATIONAL DESIGN CREATIVITY RESEARCH

As the existence of creativity is a judgment, relative to personal or group norms it is helpful to understand what factors inform that judgment. Creativity can be assessed using either the designed product or the design process. Judging the process is much more problematic than judging the product. Besemer’s Creative Product Semantic Scale for product assessment provides three dimensions, with nine attributes that can be used for judgments. As expected, “novelty” is one of the dimensions, but its three attributes provide more subtlety. This paper discusses how creative product assessment scales might provide important clues about how computational systems can be built or controlled in order to produce creative products.
Effects of Repetitive Transcranial Magnetic Stimulation on Motor Functions in Patients With Stroke: A Meta-Analysis

Background and Purpose— The purpose of this study was to perform a meta-analysis of studies that investigated the effects of repetitive transcranial magnetic stimulation (rTMS) on upper limb motor function in patients with stroke. Methods— We searched for randomized controlled trials published between January 1990 and October 2011 in PubMed, Medline, Cochrane, and CINAHL using the following key words: stroke, cerebrovascular accident, and repetitive transcranial magnetic stimulation. The mean effect size and a 95% CI were estimated for the motor outcome and motor threshold using fixed and random effect models. Results— Eighteen of the 34 candidate articles were included in this analysis. The selected studies involved a total of 392 patients. A significant effect size of 0.55 was found for motor outcome (95% CI, 0.37–0.72). Further subgroup analyses demonstrated more prominent effects for subcortical stroke (mean effect size, 0.73; 95% CI, 0.44–1.02) or studies applying low-frequency rTMS (mean effect size, 0.69; 95% CI, 0.42–0.95). Only 4 patients of the 18 articles included in this analysis reported adverse effects from rTMS. Conclusions— rTMS has a positive effect on motor recovery in patients with stroke, especially for those with subcortical stroke. Low-frequency rTMS over the unaffected hemisphere may be more beneficial than high-frequency rTMS over the affected hemisphere. Recent limited data suggest that intermittent theta-burst stimulation over the affected hemisphere might be a useful intervention. Further well-designed studies in a larger population are required to better elucidate the differential roles of various rTMS protocols in stroke treatment.
Effect of finishing technique on the occurrence and length of microcracks in resin-based materials.

PURPOSE To evaluate the presence and length of microcracks in resin-based materials finished with different techniques, using optical coherence tomography (OCT). METHODS Standardized Class V preparations (3x2x2mm) were made in the facial and lingual surfaces of 20 recently-extracted human third molars. 20 preparations were restored with a resin-based composite material (RBC; Filtek Supreme Ultra) and the other 20 with a resin-modified glass-ionomer material (RMGI; Ketac Nano). After final polymerization, specimens were further stratified by finishing system: aluminum oxide discs (Sof-Lex) or spiral fluted carbide bur series (H48L). By random allocation, each extracted tooth therefore received one RBC and one RMGI restoration, and equal numbers of restorations from each material were finished using each finishing system (n= 10). After 24 hours of storage in 100% humidity at room temperature, the specimens were evaluated at x20 to x600 under environmental SEM. Cross-sectional occlusal-cervical B-mode images were obtained in increments of 25 mm from the mesial margin to the distal margin of the restoration using a spectral-domain (SD) OCT system and analyzed using Image J software to identify and measure microcrack penetration into each restoration. The total length (mm) at the point of the deepest microcrack penetration in each specimen was recorded. Data were statistically analyzed using a t-test. RESULTS No microcracks were observed in the RBC samples. However, microcrack presence was identified in all of the RMGI specimens. The t-test showed a statistically significant difference (P< 0.05) in mean microcrack length values based on the finishing technique used for the RMGI samples. [SofLex: 0.67 (± 0.28) mm; carbide: 1.26 (± 0.30)] mm. Two-way ANOVA showed significant differences in the factors "finishing technique" and "restorative material" (P< 0.001). The interaction of these two factors was also statistically significant (P< 0.001). For the tested RMGI, Tukey post-hoc test revealed that the finishing with aluminum oxide groups resulted in statistically significant lower mean microcrack length when compared to spiral fluted carbide burs (P< 0.001). CLINICAL SIGNIFICANCE Resin-modified glass-ionomer (RMGI) is more susceptible to microcrack presence than resin-based composites. Also, aluminum oxide discs produced lower values of mean microcrack length than spiral fluted carbide burs after the finishing procedure of RMGI restorations.
Phylogeography, genetic structure and diversity in the endangered bearded vulture (Gypaetus barbatus, L.) as revealed by mitochondrial DNA

Bearded vulture populations in the Western Palearctic have experienced a severe decline during the last two centuries that has led to the near extinction of the species in Europe. In this study we analyse the sequence variation at the mitochondrial control region throughout the species range to infer its recent evolutionary history and to evaluate the current genetic status of the species. This study became possible through the extensive use of museum specimens to study populations now extinct. Phylogenetic analysis revealed the existence of two divergent mitochondrial lineages, lineage A occurring mainly in Western European populations and lineage B in African, Eastern European and Central Asian populations. The relative frequencies of haplotypes belonging to each lineage in the different populations show a steep East–West clinal distribution with maximal mixture of the two lineages in the Alps and Greece populations. A genealogical signature for population growth was found for lineage B, but not for lineage A; futhermore the Clade B haplotypes in western populations and clade A haplo‐types in eastern populations are recently derived, as revealed by their peripheral location in median‐joining haplotype networks. This phylogeographical pattern suggests allopatric differentiation of the two lineages in separate Mediterranean and African or Asian glacial refugia, followed by range expansion from the latter leading to two secondary contact suture zones in Central Europe and North Africa. High levels of among‐population differentiation were observed, although these were not correlated with geographical distance. Due to the marked genetic structure, extinction of Central European populations in the last century re‐sulted in the loss of a major portion of the genetic diversity of the species. We also found direct evidence for the effect of drift altering the genetic composition of the remnant Pyrenean population after the demographic bottleneck of the last century. Our results argue for the management of the species as a single population, given the apparent ecological exchangeability of extant stocks, and support the ongoing reintroduction of mixed ancestry birds in the Alps and planned reintroductions in Southern Spain.
Invertebrate and Microbial Colonisation in Native and Exotic Leaf Litter Species in a Mountain Stream

Decomposition of three leaf species (Alnus glutinosa, Eucalyptus globulus and Quercus robur) were examined in a headstream. During two months decomposing leaves were periodically analysed for nutrient content, soluble sugars, phenols, protein precipitation capacity, total fiber, weight loss, microbial and macroinvertebrate colonisation. The leaves of the three species showed similar patterns in dynamics of soluble sugars, tannins and phenols. Bacteria numbers per foliar dry mass were constant in oak during the experiment, but increased linearly in eucalyptus. Total heterotrophic colony forming units (bacteria and fungi) were similar in eucalyptus and oak and constant during incubation, whereas in alder, they became more abundant. The analysis of invertebrate assemblages revealed differences between alder and the other two species related to nitrogen and microbial abundance.
Supersymmetric Index In Four-Dimensional Gauge Theories

This paper is devoted to a systematic discussion of the supersymmetric index Tr (-1)^F for the minimal supersymmetric Yang-Mills theory -- with any simple gauge group G -- primarily in four spacetime dimensions. The index has refinements that probe confinement and oblique confinement and the possible spontaneous breaking of chiral symmetry and of global symmetries, such as charge conjugation, that are derived from outer automorphisms of the gauge group. Predictions for the index and its refinements are obtained on the basis of standard hypotheses about the infrared behavior of gauge theories. The predictions are confirmed via microscopic calculations which involve a Born-Oppenheimer computation of the spectrum as well as mathematical formulas involving triples of commuting elements of G and the Chern-Simons invariants of flat bundles on the three-torus.
Global increase in replication fork speed during a p57KIP2-regulated erythroid cell fate switch

The switch from self-renewal to differentiation coincides with a shorter S phase in which replication forks are faster. Cell cycle regulators are increasingly implicated in cell fate decisions, such as the acquisition or loss of pluripotency and self-renewal potential. The cell cycle mechanisms that regulate these cell fate decisions are largely unknown. We studied an S phase–dependent cell fate switch, in which murine early erythroid progenitors transition in vivo from a self-renewal state into a phase of active erythroid gene transcription and concurrent maturational cell divisions. We found that progenitors are dependent on p57KIP2-mediated slowing of replication forks for self-renewal, a novel function for cyclin-dependent kinase inhibitors. The switch to differentiation entails rapid down-regulation of p57KIP2 with a consequent global increase in replication fork speed and an abruptly shorter S phase. Our work suggests that cell cycles with specialized global DNA replication dynamics are integral to the maintenance of specific cell states and to cell fate decisions.
Evolution of locomotion in Anthropoidea: the semicircular canal evidence

Our understanding of locomotor evolution in anthropoid primates has been limited to those taxa for which good postcranial fossil material and appropriate modern analogues are available. We report the results of an analysis of semicircular canal size variation in 16 fossil anthropoid species dating from the Late Eocene to the Late Miocene, and use these data to reconstruct evolutionary changes in locomotor adaptations in anthropoid primates over the last 35 Ma. Phylogenetically informed regression analyses of semicircular canal size reveal three important aspects of anthropoid locomotor evolution: (i) the earliest anthropoid primates engaged in relatively slow locomotor behaviours, suggesting that this was the basal anthropoid pattern; (ii) platyrrhines from the Miocene of South America were relatively agile compared with earlier anthropoids; and (iii) while the last common ancestor of cercopithecoids and hominoids likely was relatively slow like earlier stem catarrhines, the results suggest that the basal crown catarrhine may have been a relatively agile animal. The latter scenario would indicate that hominoids of the later Miocene secondarily derived their relatively slow locomotor repertoires.
Does severity of HIV disease in HIV-infected mothers affect mortality and morbidity among their uninfected infants?

BACKGROUND Rates of perinatal human immunodeficiency virus (HIV) transmission are higher among HIV-infected mothers with more advanced disease, but effects of maternal disease on HIV-uninfected offspring are unclear. We investigated the hypothesis that the severity of HIV disease and immune dysfunction among mothers is associated with increased morbidity and mortality among their uninfected infants. METHODS In a birth cohort of 620 HIV-uninfected infants born to HIV-infected mothers in Lusaka, Zambia, we investigated associations between markers of more advanced maternal HIV disease and child mortality, hospital admissions, and infant weight through 4 months of age. RESULTS Mortality in the cohort of uninfected infants was 4.6% (95% confidence interval [CI], 2.8-6.3) through 4 months of age. Infants of mothers with CD4+ T cell counts of <350 cells/microL were more likely to die (hazard ratio [HR], 2.87; 95% CI, 1.03-8.03) and were more likely to be hospitalized (HR, 2.28; 95% CI, 1.17-4.45), after adjusting for other factors, including maternal death and low birth weight. The most common cause of infant death and hospitalization was pneumonia and/or sepsis. A maternal viral load of >100,000 copies/mL was associated with significantly lower child weight through 4 months of age. CONCLUSION Children born to HIV-infected mothers with advanced disease who escaped perinatal or early breastfeeding-related HIV infection are nonetheless at high risk of mortality and morbidity during the first few months of life. HIV-related immunosuppression appears to have adverse consequences for the health of infants, in addition to risks of vertical transmission.
The ScreeLing: occurrence of linguistic deficits in acute aphasia post-stroke.

OBJECTIVE To investigate the occurrence of semantic, phonological and syntactic deficits in acute aphasia with the ScreeLing after the establishment of its psychometric properties. To examine the relationship between these deficits and: (i) overall aphasia severity; and (ii) quality of Spontaneous Speech. METHODS The reliability and validity of the ScreeLing was established by investigating 141 subjects with acute aphasia (2 weeks after stroke), 23 with chronic aphasia, and 138 healthy controls. In addition, the acute patients were assessed with the Token Test and a Spontaneous Speech rating (Aphasia Severity Rating Scale). RESULTS The ScreeLing was found to be valid and reliable for assessing the presence and severity of aphasia and linguistic deficits at 12 days after stroke. In 22.4% of the patients deficits were found in only 1 of the 3 linguistic levels; phonology was most frequently disturbed (16.3%), compared with semantics (2.7%), and syntax (3.4%). The number of impaired linguistic levels was related to aphasia severity: patients with a 3-level disorder had the lowest Token Test scores; patients with a selective phonological disorder had the highest Spontaneous Speech ratings. Phonology alone explained 54.6% of the variance in the Spontaneous Speech rating. CONCLUSION In the acute stage, linguistic-level deficits are already present independently of each other, with phonology affected most frequently.
Sexual risk reduction among Zambian couples

Zambia has over 1 million HIV infections nationwide and an urban prevalence rate of 23%. This study compared the impact of male involvement in multiple and single session risk reduction interventions among inconsistent condom users in Zambia and the role of serostatus among HIV-seropositive and serodiscordant couples. Couples (N=392) were randomised into intervention arms. Among inconsistent condom users at baseline (N=83), condom use increased in both conditions and this increase was maintained over a 12-month period. At 12 months, seronegative men in the multiple session condition increased sexual barrier (male and female condoms) use in comparison with those in the single session condition (F=16.13, p=0.001) while seropositive individuals increased sexual barrier use regardless of condition. Results illustrate the importance of both single and multiple session risk reduction counselling among seronegative men in serodiscordant couples in Zambia, and highlight the differing perception of risk between seropositive and serodiscordant persons.
Increased P‐selectin gene expression in the liver vasculature and its role in the pathophysiology of neutrophil‐induced liver injury in murine endotoxin shock

We studied the role of P‐selectin, an adhesion molecule known to be important for neutrophil localization to sites of inflammation, in a model of inflammatory liver injury. Male C3Heb/ FeJ (ET‐sensitive) mice treated with 700 mg/kg galactosamine and 100 μg/kg Salmonella abortus equi endotoxin (Gal/ET), murine tumor necrosis factor α (TNF‐α,15 μg/kg), or interleukin‐1 (IL‐1, 13–23 μg/kg), showed increased P‐selectin mRNA levels in the liver. In contrast, C3H/HeJ (ET‐resistant) mice responded only to cytokines with P‐selectin mRNA formation. Whereas no P‐selectin expression was detectable in control livers, there was temporary staining of endothelium in large blood vessels but not in sinusoids between 3 and 5 h after ET, TNF‐α, or IL‐1 treatment. Severe liver injury induced by Gal/ET at 7 h was not inhibited by an anti‐P‐selectin antibody in C3Heb/FeJ mice or in P‐selectin‐deficient animals. Sequestration of neutrophils in sinusoids, i.e. those neutrophils that have been identified as critical for the injury, was not affected by the antibody or in P‐selectin‐deficient mice. However, the temporary margination in portal and postsinusoidal venules was reduced by 75% in anti‐P‐selectin antibody‐treated animals and by 51% in P‐selectin‐deficient mice. We conclude that hepatic P‐selectin gene transcription in vivo involves cytokines. However, blocking P‐selectin neither attenuated sinusoidal neutrophil sequestration nor prevented neutrophil‐induced liver injury during endotoxin shock but attenuated neutrophil margination in larger vessels. Thus, our data demonstrate similarities and fundamental differences in the requirement for adhesion molecules to localize neutrophils in the liver vasculature compared to other organs during an inflammatory response. J. Leukoc. Biol. 63: 288–296; 1998.
A 20 mV Input Boost Converter With Efficient Digital Control for Thermoelectric Energy Harvesting

This paper presents a low power boost converter for thermoelectric energy harvesting that demonstrates an efficiency that is 15% higher than the state-of-the-art for voltage conversion ratios above 20. This is achieved by utilizing a technique allowing synchronous rectification in the discontinuous conduction mode. A low-power method for input voltage monitoring is presented. The low input voltage requirements allow operation from a thermoelectric generator powered by body heat. The converter, fabricated in a 0.13 ¿m CMOS process, operates from input voltages ranging from 20 mV to 250 mV while supplying a regulated 1 V output. The converter consumes 1.6 (1.1) ¿W of quiescent power, delivers up to 25 (175) ¿W of output power, and is 46 (75)% efficient for a 20 mV and 100 mV input, respectively.
Dielectric and energy storage properties of PVDF films with large area prepared by solution tape casting process

A pilot-scale poly(vinylidene fluoride) (PVDF) film with a thickness of 2–20 μm, a width of 15 cm and a length of above 250 m was fabricated by using solution tape casting process. The crystallinity, dielectric and energy storage properties of the PVDF film were studied. The results displayed that there were predominant β phase in the PVDF film prepared at 60 °C, and coexistence of α and β phases at 80 °C. The PVDF film fabricated at 80 °C exhibited a dielectric constant of 8.7 and a dielectric loss of 3.8% at 1 kHz and room temperature. The discharged energy density calculated from D-E hysteresis loop reached to 10.1 J/cm3 with charge-discharge efficiency of 37.9% at 550 MV/m.
Ginkgo biloba Responds to Herbivory by Activating Early Signaling and Direct Defenses

Background Ginkgo biloba (Ginkgoaceae) is one of the most ancient living seed plants and is regarded as a living fossil. G. biloba has a broad spectrum of resistance or tolerance to many pathogens and herbivores because of the presence of toxic leaf compounds. Little is known about early and late events occurring in G. biloba upon herbivory. The aim of this study was to assess whether herbivory by the generalist Spodoptera littoralis was able to induce early signaling and direct defense in G. biloba by evaluating early and late responses. Methodology/Principal Findings Early and late responses in mechanically wounded leaves and in leaves damaged by S. littoralis included plasma transmembrane potential (Vm) variations, time-course changes in both cytosolic calcium concentration ([Ca2+]cyt) and H2O2 production, the regulation of genes correlated to terpenoid and flavonoid biosynthesis, the induction of direct defense compounds, and the release of volatile organic compounds (VOCs). The results show that G. biloba responded to hebivory with a significant Vm depolarization which was associated to significant increases in both [Ca2+]cyt and H2O2. Several defense genes were regulated by herbivory, including those coding for ROS scavenging enzymes and the synthesis of terpenoids and flavonoids. Metabolomic analyses revealed the herbivore-induced production of several flavonoids and VOCs. Surprisingly, no significant induction by herbivory was found for two of the most characteristic G. biloba classes of bioactive compounds; ginkgolides and bilobalides. Conclusions/Significance By studying early and late responses of G. biloba to herbivory, we provided the first evidence that this “living fossil” plant responds to herbivory with the same defense mechanisms adopted by the most recent angiosperms.