Miracle-dz/UniFAR-FTUs huggingface dataset:
Erythropoiesis in CML--immunomorphometric quantification, PCNA-reactivity, and influence on survival.

An immunohistochemical and morphometric analysis was performed on trephine biopsy specimens in 60 patients with chronic myeloid leukemia (CML) to quantify erythropoiesis and its proliferation capacity and to assess the stainable marrow iron (hemosiderin). For this purpose, an elaborate double-immunostaining technique was applied. This included a monoclonal antibody (PC10) that is directed against proliferating cell nuclear antigen (PCNA), followed by an antibody against glycophorin C (Ret40f), to identify all nucleated erythroid precursor cells. Additionally, morphometric data were derived from immunostaining of megakaryocytes (CD61) and macrophages (PG-M1), including its hemosiderin-laden subpopulation. Finally the determination of argyrophilic (reticulin) fiber density was carried out. In comparison with a control group (15 patients) without any hematologic disorder, in CML patients morphometric evaluation showed a significant reduction in the number of erythroblasts and normoblasts. This feature was associated with a PCNA-labeling index within the normal range and a decreased stainable marrow iron (number of hemosiderin-storaging macrophages). Several parameters were established to exert a predictive value on survival. A worsening of prognosis was associated with a decrease in the number of erythroid precursors (< 460/mm2), a low hemoglobin level (< 10 g/dl), a high megakaryocyte count (> 50 cells/mm2), an increased density of reticulin fibers (> 30 i x 10(2)/mm2) and splenomegaly (> 15 cm below costal margin). Our findings are in keeping with results obtained from in vitro studies of cell proliferation in CML, which is not significantly altered in comparison with the normal bone marrow. Finally, the present data, although derived from a small group of patients, emphasize the impact of histologic variables to be included in one of the major clinical trials on prognosis in CML.
Notes on Linear Approximations , Equilibrium Multiplicity and E-learnability in the Analysis of the Zero Lower Bound

We study the properties of the zero lower bound model in Eggertson andWoodford (2003) (EW). EWs analysis is based on the equilibrium conditions after linearization. Working with the actual nonlinear equilibrium conditions and consistent with Braun, Körber and Waki (2012) and Mertens and Ravn (2011), we nd the existence of equilibria that are not visible to analyses based on linearization, as well as sunspot equilibria. These ndings challenge the ndings about the properties of the zlb reported in Christiano, Eichenbaum and Rebelo (2011), Eggertson (2011), EW and others. However, we nd that the equilibria that are invisibleto analyses using linearization are not E-learnable and so those equilibria may perhaps be treated as mathematical curiosities. In addition, evidence that the quality of linear approximations is poor rests on examples where output deviates by more than 20 percent from its steady state, cases where no one would expect linear approximations to work well. For perturbations of reasonable size, the conclusions arrived at in the zlb analysis that use linear approximations appear to be robust.
Molecular Basis of Urinary Bladder Cancer

Bladder cancer is a relatively common and potentially life-threatening neoplasm. The diagnosis of urothelial carcinoma usually entails a lifelong surveillance to detect recurrent disease. In recent years, significant progress has been made in understanding the molecular mechanisms of carcinogenesis in urinary bladder. An early step in the process of carcinoma development is establishment of a premalignant abnormal urothelial patch that may give rise to various types of urothelial carcinoma and may provide a fertile ground for development of multifocal synchronous and metachronous tumors. Two distinct molecular pathways are involved. Low-grade papillary carcinoma is associated with mutation in the FGFR3 or in some cases mutations in RAS genes. High-grade in situ/muscle-invasive carcinoma on the other hand is characterized by alteration of p53 and pRB. Loss of function of these key genes, which play a crucial role in the control of cell cycle, leads to accumulation of additional mutations and deletions of genes resulting in an aggressive phenotype. It is hoped that a thorough understanding of the molecular basis of urothelial cancer will facilitate early diagnosis and will lead to development of new modalities for the management and treatment of these carcinomas.
Journal Writing Intervention to Reduce Distress Among UW -Stout Students

This study examines the effectiveness of a journal writing intervention to reduce distress levels among University of Wisconsin-Stout students. A secondary purpose of the study is to understand the impact of 'the Big Five personality traits' in moderating emotional disclosure intervention effectiveness. Information was collected from sixty-three students via self-report distress and personality measures. Students completed pre-assessment General Health 2 Questionnaire-12 (GHQ-12) and International Personality Item Pool measures. Participants were randomly assigned to either an experimental or control condition. Students within the experimental condition were required to journal regarding their recent stresses, emotions, thoughts, sources of stress, and plans to deal with their stress. Students within the control condition were required to journal concerning trivial topics. All participants were required to journal for six consecutive days, for fifteen minutes each day. Two weeks following completion
SOSYAL BILGILER DERSLERINDE ÖĞRETMEN VE ÖĞRENCI SORULARI (KIRŞEHİR ÖRNEĞİ)

Ogretim surecinde ogretmen ve ogrenciler tarafindan en cok yararlanilan ogrenme-ogretme vasitalarindan biri sorulardir. Sorulardan etkin yararlanabilmek, ogretmen ve ogrenci sorularinin nitelikleri ile iliskilidir. Bu arastirma, ogretmen ve ogrenci sorularinin nitelikleri ile aralarindaki benzerlik ve farkliliklari belirlemek uzere yapilmistir. Bu amacla, ilkogretim II. kademe sosyal bilgiler dersine giren ogretmen ve ogrencilerin ders surecinde sorduklari sorular, nitelikleri yonuyle irdelenmis; aralarindaki iliski ve farkliliklar belirlenmeye calisilmistir. Veriler, Sosyal Bilgiler Ogretmenligi Programi son sinif ogrencilerinin ilkogretim II. kademe sosyal bilgiler dersinde yaptiklari gozlemlerle toplanmistir. Arastirmanin amaci cercevesinde veriler uzerinde aritmetik ortalama, t testi, korelasyon testi, regresyon testi, anova ve LSD testi uygulanmistir. Yapilan analizler sonunda; ogretmen ve ogrenci sorularinin onemli nitelik sorunlarinin bulundugu, aralarinda anlamli farklarin ve iliskilerin bulundugu, ogrenci sorularinin ogretmen sorularindan anlamli duzeyde etkilendigi, ogretmenlerin hizmet surelerinin, sorularinin niteligi uzerinde anlamli farklilasmalara neden oldugu belirlenmistir
Cross-Cultural Differences in Social Desirability Scales: Influence of Cognitive Ability

Orientation: The use of personality tests for selection and screening has been consistently criticised resulting from the risk of socially desirable responding amongst job applicants. Research purpose: This study examined the magnitude of culture and language group meanscore differences amongst job applicants and the moderating effect of race on the relationship between social desirability and cognitive ability. Motivation for the study: The influence of cognitive ability and potential race and ethnic group differences in social desirability scale scores, which can lead to disproportional selection ratios, has not been extensively researched in South Africa. Research design, approach and method: A quantitative, cross-sectional research design, based on secondary datasets obtained from the test publisher, was employed. The dataset consisted of 1640 job applicants across industry sectors. Main findings: Moderated multiple regression analyses revealed that the relationship between social desirability and general reasoning was moderated by culture and language, with group differences in social desirability being more pronounced at the low general reasoning level. This suggests that social desirability scales may be an ambiguous indicator of faking as the scales may indicate tendency to fake, but not the ability to fake, that is likely to be connected to the level of cognitive ability of the respondent. Practical/managerial implications: Individual differences in social desirability are not fully explained by cognitive ability as cultural differences also played a role. Responding in a certain manner, reflects a level of psychological sophistication that is informed by the level of education and socio-economic status. In relation to selection practice, this study provided evidence of the potentially adverse consequences of using social desirability scales to detect response distortion. Contribution/value-add: The exploration of cross-cultural differences in the application of social desirability scales and the influence of cognitive ability is seen as a major contribution, supported by possible explanations for the differences observed and recommendations regarding the practice of universal corrections and adjustments.
Engaging Leaders in the Eyes of the Beholder

This study investigates whether work engagement mediates the relationship between transformational leadership and service climate. We also examine whether self–other agreement on transformational leadership act as a contingency to the expected relationships. Data were collected from two separate samples. The first sample consists of 1,226 employees of a financial services company while the second sample consists of 291 followers and 30 leaders from an audit company. The results support the view that work engagement mediates the relationship between transformational leadership and service climate. Polynomial regression and interaction analyses show that the relationship between transformational leadership and service climate is moderated by self–other agreement.
Two Novel doubletime Mutants Alter Circadian Properties and Eliminate the Delay between RNA and Protein inDrosophila

Phosphorylation is an important feature of pacemaker organization in Drosophila. Genetic and biochemical evidence suggests involvement of the casein kinase I homolog doubletime(dbt) in the Drosophila circadian pacemaker. We have characterized two novel dbt mutants. Both cause a lengthening of behavioral period and profoundly alterperiod (per) andtimeless (tim) transcript and protein profiles. The PER profile shows a major difference from the wild-type program only during the morning hours, consistent with a prominent role for DBT during the PER monomer degradation phase. The transcript profiles are delayed, but there is little effect on the protein accumulation profiles, resulting in the elimination of the characteristic lag between the mRNA and protein profiles. These results and others indicate that light and post-transcriptional regulation play major roles in defining the temporal properties of the protein curves and suggest that this lag is unnecessary for the feedback regulation ofper and tim protein on perand tim transcription.
Plant-Mediated Changes in the Feeding Behavior of an Invasive Whitefly

ABSTRACT The invasive whitefly Bemisia tabaci (Gennadius) is a worldwide pest of agricultural crops that feeds on a wide variety of host plants. Although host plant preference is known to vary among B. tabaci biotypes, far less is known about the potential for intraspecific divergence caused by long-term isolation on a single species of host plant. We tested the hypothesis that multigenerational isolation of B. tabaci B, a biotype that has been well-established in China for nearly two decades, on three different host plants would lead to population-level divergence in feeding behaviors. We used individuals from a cabbage-feeding (Brassica oleracea L.) population of B. tabaci B to create three populations reared exclusively on B. oleracea, cucumber (Cucumis sativus L.), or tomato (Lycopersicon esculentum Mill.) for >80 generations. We then used electrical penetration graph techniques to investigate the feeding behavior of the three B. tabaci populations on each of the three host plants (nine total treatments). Across all three host plants, the cabbage-specific population equaled or exceeded the performance of the cucumber-specific (CuSP) and tomato-specific (ToSP) populations. Strikingly, neither CuSP nor ToSP ever had the best feeding performance on their natal hosts. Our results support the hypothesis that feeding differentiation has occurred, but we found no evidence that these changes increased the feeding performance of either CuSP or ToSP. Although confirming that rapid interpopulation divergence is possible, our findings nonetheless suggest that this differentiation did not yield highly adapted populations that might pose problems for future efforts at pest management.
Malignancy rates in patients with rheumatoid arthritis treated with tocilizumab

Objective To analyse malignancy rates in patients with rheumatoid arthritis (RA) treated with tocilizumab. Methods Patients who received tocilizumab or placebo+methotrexate/disease-modifying antirheumatic drugs in the double-blind phases of 5-phase three trials or who received at least 1 dose of tocilizumab in the long-term extension studies were analysed up to the 2 May 2012 cut-off date. Malignancies were monitored throughout the studies, analysed and adjudicated as malignant by medical review. Risk was compared with that in the general population using standardised incidence ratios (SIRs) based on data from the Surveillance Epidemiology and End Results SEER (US general population) and GLOBOCAN (non-US general population) databases. Results In total, 4009 patients in the tocilizumab all-exposure population were included. Mean treatment duration was 4.0 years (mean 5.1 (range 0.0–6.8); total observation time was 16 120.1 patient-years (PY). The adjudicated malignancy rate (95% CI) was 1.26/100 PY (1.09 to 1.44) and remained constant over time. The SIR (95% CI) for all malignancies combined, excluding non-melanoma skin cancer, was 1.36 (1.01 to 1.80) for US and 1.81 (1.44 to 2.23) for non-US populations, driven primarily by higher rates in lung and bronchus (US/non-US) malignancies and prostate cancer and non-Hodgkin lymphoma (non-US), in contrast to those for the general populations; these higher rates are in line with those expected in patients with RA or in the geographic regions studied. Conclusions Malignancy rates remained stable with long-term tocilizumab treatment, and malignancy types and rates were consistent with those expected in patients with RA.
Seasonal Changes of Flavonoid Content in Melittis melissophyllum L. (Lamiaceae)

Melittis melissophyllum L. , a medicinal plant currently used in the folk medicine, was analyzed for the content of flavonoid compounds. The plants were collected in two locations in Poland in May and September. MeOH Extracts from the leaves and flowers (separately) were analyzed by HPLC‐DAD. Eight compounds were identified in all the samples and quantitatively analyzed as cinaroside (=luteolin 7‐O‐glucoside), rutin, myricetin, quercitrin, quercetin, luteolin, kaempferol, and apigenin. M. melissophyllum accumulated the highest total amounts of flavonoids in May (flowers: from 258 to 271 mg/100 g dry weight (dw); leaves: from 143 to 155 mg/100 g dw) and significantly lower ones in September (leaves: from 83 to 92 mg/100 g dw). The main compound was cinaroside (May: up to 249 mg/100 g dw; September: up to 43 mg/100 g dw). Advanced multivariate statistical techniques (cluster analysis (CA) and principal component analysis (PCA)) were used to characterize the sample populations and to analyze the data. We report, for the first time, the results of the quantitative analysis of M. melissophyllum flavonoids and seasonal changes in their accumulation. Our results show that the time of harvesting has a significant influence on the flavonoid content in M. melissophyllum, while the geographical location does not have such an effect.
The press crisis and its impact on Swedish arts journalism: Autonomy loss, a shifting paradigm and a ‘journalistification’ of the profession

Research indicates a crisis in the journalistic field due to economic, technological, institutional and cultural shifts. This seems to have led to a paradigm shift in arts journalism, from a cultural to a journalistic focus, and a loss of autonomy for culture departments in the Swedish press. This article investigates how arts journalists’ professional values are affected by this presumed autonomy loss, the paradigm shift and insecure working conditions due to managerial control and economic pressure. The research material consists of interviews and articles by editors and critics debating their profession. The results show that the ideal-typical values traditionally associated with journalism in Western democratic society are under pressure. This prompts a redefinition of the profession and debate about the future of arts journalism and its threatened role of promoting cultural heritage and participation in an autonomous cultural public sphere.
Structural investigations into the interaction of hemoglobin and part structures with bacterial endotoxins

An understanding of details of the interaction mechanisms of bacterial endotoxins (lipopolysaccharide, LPS) with the oxygen transport protein hemoglobin is still lacking, despite its high biological relevance. Here, a biophysical investigation into the endotoxin:hemoglobin interaction is presented which comprises the use of various rough mutant LPS as well as free lipid A; in addition to the complete hemoglobin molecule from fetal sheep extract, also the partial structure α-chain and the heme-free sample are studied. The investigations comprise the determination of the gel-to-liquid crystalline phase behaviour of the acyl chains of LPS, the ultrastructure (type of aggregate structure and morphology) of the endotoxins, and the incorporation of the hemoglobins into artificial immune cell membranes and into LPS. Our data suggest a model for the interaction between Hb and LPS in which hemoglobins do not react strongly with the hydrophilic or with the hydrophobic moiety of LPS, but with the complete endotoxin aggregate. Hb is able to incorporate into LPS with the longitudinal direction parallel to the lipid A double-layer. Although this does not lead to a strong disturbance of the LPS acyl chain packing, the change of the curvature leads to a slightly conical molecular shape with a change of the three-dimensional arrangement from unilamellar into cubic LPS aggregates. Our previous results show that cubic LPS structures exhibit strong endotoxic activity. The property of Hb on the physical state of LPS described here may explain the observation of an increase in LPS-mediating endotoxicity due to the action of Hb.
Impact of increased penetration of large-scale wind farms on power system dynamic stability - A review

Increased penetration of wind farms has created a new security concern for transmission system operators (TSOs) regarding the dynamic stability of the system. The renewable energy sources (RES) with more intermittent characteristics and lower inertial response can have considerable effect on the dynamic behavior of the conventional power systems, and as a result the characteristics of the oscillations may be significantly impacted. There are many research in literature that investigate the impact of wind penetration on dynamic stability of the power system, with even contradictory findings reported by different authors. This paper, based on most recent literature, presents a comprehensive survey on the impact of high penetration of wind farms on dynamic stability of power systems in particular on the small-signal and transient rotor angle stability. Even though, there cannot be a general statement made as an answer to the problem under consideration, however, this paper has drawn several important conclusions that must be carefully taken into account when integrating wind power into existing power systems.
SRC-family tyrosine kinases in wound- and ligand-induced epidermal growth factor receptor activation in human corneal epithelial cells.

PURPOSE The authors have previously demonstrated that wounding of human corneal epithelial cells (HCECs) transactivates epidermal growth factor (EGF) receptor (EGFR) and its downstream signaling pathways and that this EGFR signaling is required for epithelial wound healing. In this study, the authors sought to identify the underlying mechanisms for EGFR transactivation in response to wounding in HCECs. METHODS SV40-immortalized HCEC (THCE) monolayer was wounded and allowed to heal in the presence or absence of a selective inhibitor of the Src family kinases PP2 and EGFR ligand heparin-binding EGF-like growth factor (HB-EGF). Wound closure was monitored by photographing of the injury immediately or 24 hours after wounding. Activation of EGFR in THCE cells and in primary HCECs was analyzed by immunoprecipitation of EGFR, followed by Western blotting with phosphotyrosine antibody. Phosphorylation of extracellular signal-regulated kinase (ERK), AKT (a major substrate of phosphatidylinositol 3'-kinase [PI3K]), Src at tyrosine Y416, and EGFR at Y845 was analyzed by Western blotting with antibodies specific to phosphorylated proteins. Effects of PP2 on THCE cell migration were determined by Boyden chamber migration assay. RESULTS Among several inhibitors tested, PP2 blocked wound-induced EGFR phosphorylation in THCE cells. PP2 at 12.5 microM effectively inhibited EGFR transactivation in response to wounding and to the phosphorylation of ERK and AKT in THCE cells and primary HCECs. Consistent with the inhibition of EGFR transactivation, PP2 also attenuated epithelial migration and wound closure with or without exogenously added HB-EGF. PP2 at a concentration as high as 50 microM exhibited no effects on HB-EGF induced ERK phosphorylation. On the other hand, AKT phosphorylation was much more sensitive to PP2 than ERK or EGFR phosphorylation because 3.13 microM PP2 effectively inhibited wound- or HB-EGF-induced AKT phosphorylation. CONCLUSIONS These results suggest that Src kinase mediates wound-induced EGFR transactivation and participates in a pathway to activate the PI3K-AKT pathway downstream of EGFR in HCECs.
Good Guys With Guns

In most states in the U.S. it is legal to carry a concealed handgun in public, but little is known about why people want to do this. While the existing literature argues that guns symbolize masculinity, most research on the actual use of guns has focused on marginalized men. The issue of concealed handguns is interesting because they must remain concealed and because relatively privileged men are most likely to have a license to carry one. Using in-depth interviews with 20 men, this article explores how they draw on discourses of masculinity to explain their use of concealed handguns. These men claim that they are motivated by a desire to protect their wives and children, to compensate for lost strength as they age, and to defend themselves against people and places they perceive as dangerous, especially those involving racial/ethnic minority men. These findings suggest that part of the appeal of carrying a concealed firearm is that it allows men to identify with hegemonic masculinity through fantasies of violence and self-defense.