Miracle-dz/UniFAR-FTUs huggingface dataset:
The Nanos3-3′UTR Is Required for Germ Cell Specific NANOS3 Expression in Mouse Embryos

Background The regulation of gene expression via a 3′ untranslated region (UTR) plays essential roles in the discrimination of the germ cell lineage from somatic cells during embryogenesis. This is fundamental to the continuation of a species. Mouse NANOS3 is an essential protein required for the germ cell maintenance and is specifically expressed in these cells. However, the regulatory mechanisms that restrict the expression of this gene in the germ cells is largely unknown at present. Methodology/Principal Findings In our current study, we show that differences in the stability of Nanos3 mRNA between germ cells and somatic cells is brought about in a 3′UTR-dependent manner in mouse embryos. Although Nanos3 is transcribed in both cell lineages, it is efficiently translated only in the germ lineage. We also find that the translational suppression of NANOS3 in somatic cells is caused by a 3′UTR-mediated mRNA destabilizing mechanism. Surprisingly, even when under the control of the CAG promoter which induces strong ubiquitous transcription in both germ cells and somatic cells, the addition of the Nanos3-3′UTR sequence to the coding region of exogenous gene was effective in restricting protein expression in germ cells. Conclusions/Significance Our current study thus suggests that Nanos3-3′UTR has an essential role in translational control in the mouse embryo.
Research in Education ( CPRE ) 7-2012 Measuring School Capacity , Maximizing School Improvement

Given the nearly ubiquitous use of the term "capacity" in education policy discourse, this policy brief offers a common framework for analyzing capacity that educators, policymakers, and researchers alike can apply and understand with consistency. Drawing data from a larger three-year CPRE study of school responses to accountability in Pennsylvania, the authors' goal is not to provide an easy, new, one-sentence definition, but rather to create a shared language that can be applied to research and improvement efforts in schools. To accomplish this, the authors break capacity down into component parts, explaining how each one builds off the next and contributes to theoverall concept. Disciplines Educational Assessment, Evaluation, and Research | Education Policy Comments View on the CPRE website. This policy brief is available at ScholarlyCommons: http://repository.upenn. edu/cpre_policybriefs/41
Human Leukocyte Antigen Class I and II Alleles and Overall Survival in Diffuse Large B-Cell Lymphoma and Follicular Lymphoma

Genetic variation in the 6p21 chromosomal region, including human leukocyte antigen (HLA) genes and tumor necrosis factor (TNF), has been linked to both etiology and clinical outcomes of lymphomas. We estimated the effects of HLA class I (A, B, and C), class II DRB1 alleles, and the ancestral haplotype (AH) 8.1 (HLAA*01-B*08-DRB1*03-TNF-308A) on overall survival (OS) among patients with diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) in a population-based study of non-Hodgkin lymphoma. During a median followup of 89 months, 31% (52 of 166) DLBCL and 28% (46 of 165) FL patients died. Using multivariate Cox regression models, we observed statistically significant associations between genetic variants and survival: HLA-Cw*07:01 was associated with poorer OS among DLBCL patients (Hazard ratio [HR] = 1.76, 95% confidence interval [CI] = 1.01–3.05); HLA-A*01:01 was associated with poorer OS (HR = 2.23, 95% CI = 1.24–4.01), and HLA-DRB1*13 (HR = 0.12, 95% CI = 0.02–0.90) and HLA-B Bw4 (HR = 0.36, 95% CI = 0.20–0.63) with better OS among FL patients. These results support a role for HLA in the prognosis of DLBCL and FL and represent a promising class of prognostic factors that warrants further evaluation.
The influence of mood and cognitive load on driver performance: using multiple measures to assess safety

Emotions and moods are an inevitable part of human life. Previous research suggests that positive and negative moods affect human performance in many aspects: decision making, perception, reasoning and memory. The influence of mood on drivers’ behaviour has been studied to a less extent and mainly with respect to negative emotions. The studies reported in this thesis are based on psychological theories regarding the differences in the effects of positive and negative moods on information processing and mind wandering. The thesis describes two studies: a desktop study and a driving simulator study, which measure drivers’ responses to the actions of other traffic, their observational patterns and driving behaviours in a variety of scenarios. The effects of neutral, happy, sad and angry moods were studied. The simulator study also investigated possible ways to disconnect drivers’ minds from mood induced mind wandering by using different types of cognitive load. The results suggest that mood valence and arousal have different effects on driving safety, with negative moods resulting in the most dangerous driving. In order to draw conclusions about the effect of mood, a combination of multiple measures (e.g. glance patterns, driving performance and drivers’ physiological measures) should be analysed. The results also suggest that some amount of cognitive load, applied while driving, can have a positive effect on drivers’ attention. Further research is needed to establish the amount and type of the cognitive load necessary to improve drivers’ ability to maintain their attention on the driving task. Studies with a larger number of participants and field studies are needed to validate the findings. It is suggested that the findings are used to improve in-car assistance systems able to both detect the harmful effects of a driver’s emotional state and re-direct their attention to the primary task of driving.
The free-rider problem and the theory of the corporation

A spray gun especially adapted for dispensing foam type plastics or other coating materials which is characterized by two feed lines for the coating materials which are encased in return lines with valves which are selectively positioned to control the flow of the material so as to provide, selectively, for spray operation or for continuous return flow to the supply source without passage through the discharge control valves of the gun. The discharge control valve assemblies are readily removable as complete units for cleaning and repair. A solvent flush valve is arranged relative to the discharge control valves to enable the valves, the mixing chamber and passageways in the head to be flushed out with solvent while the valves are in place and while the coating material is recirculated through the supply lines.
The redshift-space two-point correlation function of ELAIS-S1 galaxies

We investigate the clustering properties of galaxies in the recently completed ELAIS-S 1 redshift survey through their spatial two-point autocorrelation function. We used a subsample of the ELAIS-S 1 catalogue covering approximately 4 deg 2 and consisting of 148 objects selected at 15 μm with a flux >0.5 mJy and a redshift z < 0.5. We detected a positive signal in the correlation function that in the range of separations 1-10 h -1 Mpc is well approximated by a power law with a slope y = 1.4 ± 0.25 and a correlation length s 0 = 5.4 ± 1.2 h -1 Mpc, at the 90 per cent significance level. This result is in good agreement with the redshift-space correlation function measured in more local samples of mid-infrared-selected galaxies such as the IRAS Point Source Catalog (PSCz) redshift survey. This suggests a lack of significant clustering evolution of infrared-selected objects out to z = 0.5 that is further confirmed by the consistency found between the correlation functions measured in a local (z < 0.2) and a distant (0.2 < z < 0.5) subsample of ELAIS-S 1 galaxies. We also confirm that optically selected galaxies in the local redshift surveys, especially those of the SDSS sample, are significantly more clustered than infrared objects.
A Review of Temperature, pH, and Other Factors that Influence the Survival of Salmonella in Mayonnaise and Other Raw Egg Products

Salmonellosis is one of the main causes of foodborne illnesses worldwide, with outbreaks predominately linked to contamination of eggs and raw egg products, such as mayonnaise. This review explores previous studies that have investigated Salmonella control mechanisms utilized in the production of raw egg mayonnaise and other food products. Apart from the use of pasteurized eggs, the main control mechanism identified is the pH of the raw egg products, which plays an important role in the consistency and stability while affecting the survival of Salmonella spp. However, currently there is no consensus regarding the critical pH limit for the control of Salmonella. The effectiveness of pH as a control mechanism is influenced by the type of acid used, with the effectiveness of lemon juice compared with vinegar highly debated. Additionally, Salmonella susceptibility to pH stresses may also be influenced by storage temperature (in some studies refrigeration temperatures protected Salmonella spp. from acidulants) and is further complicated by the development of Salmonella cross-tolerance-induced responses, pH homeostasis achieved by the cellular antiport and symport systems, and acid tolerance response (ATR). These mechanisms all provide Salmonella with an added advantage to ensure survival under various pH conditions. Other confounding factors include the fat content, and the addition of NaCl, garlic and plant essential oils (PEOs) from mint, cinnamon, cardamom and clove.
Ipsilesional High Frequency Repetitive Transcranial Magnetic Stimulation Add-On Therapy Improved Diffusion Parameters of Stroke Patients with Motor Dysfunction: A Preliminary DTI Study

Purpose. The aim of this study was to evaluate the effects of high frequency repetitive transcranial magnetic stimulation (HF-rTMS) on stroke patients with motor dysfunction and to investigate the underlying neural mechanism. Methods. Fifteen stroke patients were assigned to the rTMS treatment (RT) group and conventional treatment (CT) group. Patients in the RT received 10 Hz rTMS stimulation on the ipsilesional primary motor cortex for 10 days plus conventional treatment of CT, which consisted of acupuncture and antiplatelet aggregation medication. Difference in fractional anisotropy (FA) between pretreatment and posttreatment and between two groups was determined. Correlations between FA values and neurological assessments were also calculated. Results. Both groups significantly improved the neurological function after treatment. rTMS-treated patients showed better improvement in Fugl-Meyer Assessment (FMA) score and increased FA value in motor-related white matter and gray matter cortices compared with CT-treated patients and pretreatment status. Besides, the increased FA value in the ipsilesional posterior limb of the internal capsule in RT group was significantly correlated with the improved FMA score. Significance. HF-rTMS could be a supplement therapy to CT in improving motor recovery in patients with stroke. And this benefit effect may be achieved through modulating the ipsilesional corticospinal tracts and motor-related gray matter cortices.
Auditory event-related potentials as a function of abstract change magnitude

Event-related potentials were recorded in healthy volunteers to test the accuracy of the human brain to extract, preattentively, auditory abstract rules. The abstract rule was determined by the frequency relationship between two pure tones forming a pair. The standard pairs had identical tone frequency, whereas the deviant pairs had the second tone two, four, six or eight musical steps higher or lower in frequency than the first one. All abstract changes elicited mismatch negativity, which was not affected by the magnitude of change. However, the subsequent P3a increased as a function of the magnitude of the abstract change. These results suggest that mismatch negativity detects violations of abstract rules, and the amount of violation is analyzed in subsequent stages of auditory processing.
A Two-Stage Dynamic Model for Visual Tracking

We propose a new dynamic model which can be used within blob trackers to track the target's center of gravity. A strong point of the model is that it is designed to track a variety of motions which are usually encountered in applications such as pedestrian tracking, hand tracking, and sports. We call the dynamic model a two-stage dynamic model due to its particular structure, which is a composition of two models: a liberal model and a conservative model. The liberal model allows larger perturbations in the target's dynamics and is able to account for motions in between the random-walk dynamics and the nearly constant-velocity dynamics. On the other hand, the conservative model assumes smaller perturbations and is used to further constrain the liberal model to the target's current dynamics. We implement the two-stage dynamic model in a two-stage probabilistic tracker based on the particle filter and apply it to two separate examples of blob tracking: 1) tracking entire persons and 2) tracking of a person's hands. Experiments show that, in comparison to the widely used models, the proposed two-stage dynamic model allows tracking with smaller number of particles in the particle filter (e.g., 25 particles), while achieving smaller errors in the state estimation and a smaller failure rate. The results suggest that the improved performance comes from the model's ability to actively adapt to the target's motion during tracking.
Failure to Protect: America's Sexual Predator Laws and the Rise of the Preventive State

This posting contains the introduction and one chapter from my new book 'Failure to Protect: America's Sexual Predator Laws and the Rise of the Preventive State' (Cornell University Press 2006). Most crimes of sexual violence are committed by people known to the victim - acquaintances and family members. Yet politicians and the media overemphasize predatory strangers when legislating against and reporting on sexual violence. In this book, I seek to look beyond sensational headlines to expose the reality of the laws designed to prevent sexual crimes. I show that sexual predatory laws, which have intense public and political support, are counterproductive. I contend that measures such as civil commitment and Megan's law, which are designed to restrain sex offenders before they commit another crime, are bad policy and do little to reduce sexual violence. Further, these new laws make use of approaches such as preventative detention and actuarial profiling that violate important principles of liberty. I argue that to prevent sexual violence, policymakers must address the deep-seated societal problems that allow it to flourish. In addition to criminal sanctions, I endorse the specific efforts of some advocates, organizations, and social scientists to stop sexual violence by, for example, taking steps to change the attitudes and behaviors of school-age children and adolescents, improving public education and promoting community treatment and supervision of previous offenders. I also warn that the principles underlying the predator laws may be the harbingers of a preventive state in which the government casts nets of surveillance and intervenes to curtail liberty before crimes occur. I also show that the architecture of sexual violence that these new legal approaches nurture is at odds with the advances promoted by feminist thinkers during the last several decades. More than a critique of the status quo, this book discusses alternatives and how best to overcome the political obstacles to achieving rational policy.
T cell traffic and the inflammatory response in experimental autoimmune uveoretinitis.

PURPOSE To quantify S-antigen-specific (S-Ag) T cells in the retina after adoptive transfer, and to evaluate their role in the initiation and progress of destructive ocular inflammation in experimental autoimmune uveoretinitis (EAU). METHODS Lewis rats were administered 10 x 10(6) S-Ag-specific T cells from the SP35 cell line or 10 x 10(6) concanavalin A-stimulated syngeneic spleen cell lymphoblasts labeled with lipophilic PKH26 fluorescent dye immediately before intravenous inoculation. Labeled cells in each retina were counted at various times from 4 to 120 hours after cell transfer by fluorescence microscopic analysis of each dissociated retina. Recipient eyes were examined within the same period by light and confocal microscope. RESULTS SP35 T cells showed a biphasic distribution in the retina. The first peak of 160 cells/retina was noted at 24 hours. A steady decline of labeled cells at 48 and 72 hours was followed by a rapid increase at 96 and 120 hours. Concanavalin A-stimulated, control-labeled cell populations showed an identical peak at 24 hours but a persistent decline thereafter; only two or three T cells were present in each retina at 120 hours. Concurrent inoculation of SP35 cells and nonspecific T cell blasts did not produce more SP35 cells than control cells in the retina at any time. Microscopic analysis showed mononuclear cell infiltration of the iris, ciliary body, and aqueous humor at 48 hours, which intensified rapidly and persisted through 120 hours. Retinal inflammation did not begin until 80 hours. Mononuclear cell adherence to vascular endothelium and perivascular macrophage infiltration of the innermost layers progressed to edema, and profound destructive inflammation and loss of retinal stratification were observed at 120 hours. CONCLUSIONS There is no evidence of a blood-ocular or blood-retinal barrier to activated T cell blasts. Autologous S-Ag does not provoke a more rapid entry of specific T cells at that site. The data confirm that anterior segment inflammation precedes retinal inflammation, even though S-Ag-specific T cells were present in the retina within a few hours after cell transfer. Because S-Ag is clearly present in the retina, delay in antigen presentation at that site may account for the temporal difference between retinal and anterior segment inflammation.
Hostility and Physiological Responses to Acute Stress in People With Type 2 Diabetes

Objective Hostility is associated with cardiovascular mortality and morbidity, and one of the mechanisms may involve heightened reactivity to mental stress. However, little research has been conducted in populations at high risk for cardiovascular disease. The aim of the present study was to assess the relationship between hostility and acute stress responsivity in individuals with Type 2 diabetes. Methods A total of 140 individuals (median age [standard deviation] 63.71 [7.00] years) with Type 2 diabetes took part in laboratory-based experimental stress testing. Systolic blood pressure, diastolic blood pressure, heart rate, plasma interleukin-6 (IL-6), and salivary cortisol were assessed at baseline, during two stress tasks, and 45 and 75 minutes later. Cynical hostility was assessed using the Cook Medley Cynical Hostility Scale. Results Participants with greater hostility scores had heightened increases in IL-6 induced by the acute stress tasks (B = 0.082, p = .002), independent of age, sex, body mass index, smoking, household income, time of testing, medication, and baseline IL-6. Hostility was inversely associated with cortisol output poststress (B = −0.017, p = .002), independent of covariates. No associations between hostility and blood pressure or heart rate responses were observed. Conclusions Hostile individuals with Type 2 diabetes may be susceptible to stress-induced increases in inflammation. Further research is needed to understand if such changes increase the risk of cardiovascular disease in this population.
Interaction of Kinase-Interaction-Motif Protein Tyrosine Phosphatases with the Mitogen-Activated Protein Kinase ERK2

The mitogen-activation protein kinase ERK2 is tightly regulated by multiple phosphatases, including those of the kinase interaction motif (KIM) PTP family (STEP, PTPSL and HePTP). Here, we use small angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC) to show that the ERK2:STEP complex is compact and that residues outside the canonical KIM motif of STEP contribute to ERK2 binding. Furthermore, we analyzed the interaction of PTPSL with ERK2 showing that residues outside of the canonical KIM motif also contribute to ERK2 binding. The integration of this work with previous studies provides a quantitative and structural map of how the members of a single family of regulators, the KIM-PTPs, differentially interact with their corresponding MAPKs, ERK2 and p38α.
Rolling-Circle Transposons Catalyze Genomic Innovation in a Mammalian Lineage

Rolling-circle transposons (Helitrons) are a newly discovered group of mobile DNA widespread in plant and invertebrate genomes but limited to the bat family Vespertilionidae among mammals. Little is known about the long-term impact of Helitron activity because the genomes where Helitron activity has been extensively studied are predominated by young families. Here, we report a comprehensive catalog of vetted Helitrons from the 7× Myotis lucifugus genome assembly. To estimate the timing of transposition, we scored presence/absence across related vespertilionid genome sequences with estimated divergence times. This analysis revealed that the Helibat family has been a persistent source of genomic innovation throughout the vespertilionid diversification from approximately 30–36 Ma to as recently as approximately 1.8–6 Ma. This is the first report of persistent Helitron transposition over an extended evolutionary timeframe. These findings illustrate that the pattern of Helitron activity is akin to the vertical persistence of LINE retrotransposons in primates and other mammalian lineages. Like retrotransposition in primates, rolling-circle transposition has generated lineage-specific variation and accounts for approximately 110 Mb, approximately 6% of the genome of M. lucifugus. The Helitrons carry a heterogeneous assortment of host sequence including retroposed messenger RNAs, retrotransposons, DNA transposons, as well as introns, exons and regulatory regions (promoters, 5′-untranslated regions [UTRs], and 3′-UTRs) of which some are evolving in a pattern suggestive of purifying selection. Evidence that Helitrons have contributed putative promoters, exons, splice sites, polyadenylation sites, and microRNA-binding sites to transcripts otherwise conserved across mammals is presented, and the implication of Helitron activity to innovation in these unique mammals is discussed.