Miracle-dz/UniFAR-FTUs huggingface dataset:
Alanine-glyoxylate aminotransferase 2 (AGXT2) Polymorphisms Have Considerable Impact on Methylarginine and β-aminoisobutyrate Metabolism in Healthy Volunteers

Elevated plasma concentrations of asymmetric (ADMA) and symmetric (SDMA) dimethylarginine have repeatedly been linked to adverse clinical outcomes. Both methylarginines are substrates of alanine-glyoxylate aminotransferase 2 (AGXT2). It was the aim of the present study to simultaneously investigate the functional relevance and relative contributions of common AGXT2 single nucleotide polymorphisms (SNPs) to plasma and urinary concentrations of methylarginines as well as β-aminoisobutyrate (BAIB), a prototypic substrate of AGXT2. In a cohort of 400 healthy volunteers ADMA, SDMA and BAIB concentrations were determined in plasma and urine using HPLC-MS/MS and were related to the coding AGXT2 SNPs rs37369 (p.Val140Ile) and rs16899974 (p.Val498Leu). Volunteers heterozygous or homozygous for the AGXT2 SNP rs37369 had higher SDMA plasma concentrations by 5% and 20% (p = 0.002) as well as higher BAIB concentrations by 54% and 146%, respectively, in plasma and 237% and 1661%, respectively, in urine (both p<0.001). ADMA concentrations were not affected by both SNPs. A haplotype analysis revealed that the second investigated AGXT2 SNP rs16899974, which was not significantly linked to the other AGXT2 SNP, further aggravates the effect of rs37369 with respect to BAIB concentrations in plasma and urine. To investigate the impact of the amino acid exchange p.Val140Ile, we established human embryonic kidney cell lines stably overexpressing wild-type or mutant (p.Val140Ile) AGXT2 protein and assessed enzyme activity using BAIB and stable-isotope labeled [2H6]-SDMA as substrate. In vitro, the amino acid exchange of the mutant protein resulted in a significantly lower enzyme activity compared to wild-type AGXT2 (p<0.05). In silico modeling of the SNPs indicated reduced enzyme stability and substrate binding. In conclusion, SNPs of AGXT2 affect plasma as well as urinary BAIB and SDMA concentrations linking methylarginine metabolism to the common genetic trait of hyper-β-aminoisobutyric aciduria.
SPECIES DIVERSITY AND SEASONAL ABUNDANCE OF SCARABAEOID DUNG BEETLES (COLEOPTERA: SCARABAEIDAE, GEOTRUPIDAE AND TROGIDAE) ATTRACTED TO COW DUNG IN CENTRAL NEW JERSEY

Abstract Species diversity and abundance of scarabaeoid dung beetles (Coleoptera) attracted to fresh cow dung were studied in three habitats of New Jersey: Hutcheson Memorial Forest (HMF) disturbed field, HMF old growth forest, and Rutgers University Bovine Farm. Over a one year period, baited pitfall traps yielded a total of 15,206 beetles representing at least 26 species. Onthophagus hecate was a dominant species in all three sites, accounting for 55.1% of all individuals collected. Onthophagus pennsylvanicus and Copris minutus were present in high numbers in the field, comprising 25.1% and 3.8%, respectively, of specimens collected in that habitat, while O. orpheus and C. minutus were numerous in the forest (20.8% and 13.3%, respectively). Two introduced species, Aphodius lividus (68.5%) and O. taurus (9.6%), were the most numerous species on the farm. Nine species accounted for more than 96% of all scarabaeoid dung beetles collected during the year-long study. The majority of the beetles were collected during the warmer months (May–September), with general peaks appearing to be correlated with temperature. A total of five introduced species were collected: five in the farm site, two in the field site, but none in the forest; 80% of the individuals collected on the farm were introduced.
Physical and Psychological Health Status of North Korean Defector Children

The purpose of the study was to evaluate the physical and psychological health status of North Korean defector children and identify associated factors. The participants in the study were 103 children ranging in age from 7 to 14 years old. Physical health status was evaluated through height and weight measurements as well as clinical laboratory results. Psychological health status was assessed using structured survey interviews. Data were analyzed utilizing Pearson correlation coefficients, t-test, ANOVA, and descriptive statistics. On arrival in South Korea, 19% of the children did not meet the child growth standards of South Koreans for height and 15% for weight. However, twelve weeks after, these percentages had decreased significantly. It found that 38.5% of children presented mild posttraumatic reaction and 40.4% presented moderate reaction. Scores for externalizing and internalizing behavior problems were 0.38±0.30 and 0.59±0.36 respectively. Posttraumatic reaction was higher in girls (t=0.41, p=.03), and boys showed higher externalizing problems (t=2.04, p=.04). Externalizing problems were correlated with gender and internalizing problems were associated with posttraumatic reactions and externalizing problems. The findings indicate that North Korean defector children need timely developmental assessments and tailored intervention programs to meet child growth standards need to be developed.
Suggestion of concomitant changes of electric power consumption and childhood leukemia in Greece

Over a 14-year period (1976–89) 679 deaths from childhood leukemia were registered in Greece and the corresponding mortality over this period declined by almost 70%, with no evidence of differential reduction by gender or population type. For each of the nine geographical regions of the country, slopes of decreasing mortality from childhood leukemia over the study period were calculated and correlated with the corresponding slopes of increasing electric power consumption over the 16-year period 1970–85 (allowing for a postulated latency of about 5 years). A positive association was noted, which however was not statistically significant (p∼0.26). Studies of similar nature conducted in larger countries over more extended periods could contribute to the resolution of the controversy surrounding the role of electric power-generated extremely low frequency electric and magnetic fields in the etiology of childhood leukemia.
Case‐relevant vs. case‐irrelevant questioning in experimental lie detection

Observers (N = 121) judged the veracity of four communicators. Before making veracity judgments, observers viewed one of three baseline interview segments for each communicator: (1) a case‐relevant segment, (2) a case‐irrelevant segment, or (3) both case‐relevant and irrelevant segments. Observers were also cued to use one of three judgmental priming cues while making their veracity attribution. Specifically, observers were instructed to pay close attention to another's: (1) verbal message (plausibility), (2) nonverbal message, or (3) nervousness. A fourth control group of observers were not cued to use any specific cue while watching the videotapes. It was predicted that observers watching a case‐relevant baseline interview would detect deception more accurately than observers watching a case‐irrelevant baseline interview. Results indicated that individuals who viewed a case‐relevant baseline segment before judging deception were more accurate than individuals who viewed a case‐irrelevant baseline segmen.
Adapting the Cognitive Test Anxiety Scale for use with Argentinean University Students

A new Spanish version of the Cognitive Test Anxiety Scale (CTAS) was created to be used explicitly with Argentinean university students. The scale was translated and verified through blind back translation and given to a large sample of students majoring in psychology or chemistry (N = 752). Exploratory Factor Analysis (N = 376) showed an internal structure of two factors that differed from the established English version of the CTAS. Examination of the items revealed that the factors were likely influenced by the phrasing of items that were originally designed to have several items require endorsement of low anxiety. Confirmatory factor analyses (N = 376) were conducted to compare the fit of three models for the scale. The results demonstrated that a 16-item single-factor solution was the preferable model. Further analyses demonstrated strong internal consistency, and test-retest stability of the short Spanish version. Results support the utility of the scale in future transcultural research on test anxiety with American and Argentinean learners.
Effects of Stem Orientation and Completeness of Multiple-Choice Items on Item Difficulty and Discrimination

The effects of stem orientation (positively stated stem or negatively stated stem) and completeness (complete stem or incomplete stem) of multiple-choice items were studied experimentally. Provisions were also made for determining possible interactions between orientation, completeness, and achievement. Thus, subjects (142 senior education students) were classified into three achievement groups (Low, Medium, High). A three-way factorial design (orientation x completeness x achievement) was used as the experimental model. Results indicated that incomplete versus complete stems increased item difficulty but had no effect on discrimination. Stem orientation had no effect on either difficulty or discrimination. Neither orientation nor completeness interacted with achievement or with each other. The implications of the results are discussed.
Metric Tests for Curvature from Weak Lensing and Baryon Acoustic Oscillations

We describe a practical measurement of the curvature of the universe which, unlike current constraints, relies purely on the properties of the Robertson-Walker metric rather than any assumed model for the dynamics and content of the universe. The observable quantity is the cross-correlation between foreground mass and gravitational shear of background galaxies, which depends on the angular diameter distances dA(zl), dA(zs), and dA(zs,zl) on the degenerate triangle formed by observer, source, and lens. In a flat universe, dA(zl,zs) = dA(zs) - dA(zl), but in curved universes an additional term ∝Ωk appears and alters the lensing observables even if dA(z) is fixed. We describe a method whereby weak-lensing data can be used to solve simultaneously for dA and the curvature. This method is completely insensitive to the equation of state of the contents of the universe, or amendments to general relativity that alter the gravitational deflection of light or the growth of structure. The curvature estimate is also independent of biases in the photometric redshift scale. This measurement is shown to be subject to a degeneracy among dA, Ωk, and the galaxy bias factors that may be broken by using the same imaging data to measure the angular scale of baryon acoustic oscillations. Simplified estimates of the accuracy attainable by this method indicate that ambitious weak-lensing + baryon-oscillation surveys would measure Ωk to an accuracy ≈0.04f(σln z/0.04)1/2, where σln z is the photometric redshift error. The Fisher-matrix formalism developed here is also useful for predicting bounds on curvature and other characteristics of parametric dark energy models. We forecast some representative error levels and compare ours to other analyses of the weak-lensing cross-correlation method. We find both curvature and parametric constraints to be surprisingly insensitive to the systematic shear calibration errors.
Inhibition of the cAMP/PKA/CREB Pathway Contributes to the Analgesic Effects of Electroacupuncture in the Anterior Cingulate Cortex in a Rat Pain Memory Model

Pain memory is considered as endopathic factor underlying stubborn chronic pain. Our previous study demonstrated that electroacupuncture (EA) can alleviate retrieval of pain memory. This study was designed to observe the different effects between EA and indomethacin (a kind of nonsteroid anti-inflammatory drugs, NSAIDs) in a rat pain memory model. To explore the critical role of protein kinase A (PKA) in pain memory, a PKA inhibitor was microinjected into anterior cingulate cortex (ACC) in model rats. We further investigated the roles of the cyclic adenosine monophosphate (cAMP), PKA, cAMP response element-binding protein (CREB), and cAMP/PKA/CREB pathway in pain memory to explore the potential molecular mechanism. The results showed that EA alleviates the retrieval of pain memory while indomethacin failed. Intra-ACC microinjection of a PKA inhibitor blocked the occurrence of pain memory. EA reduced the activation of cAMP, PKA, and CREB and the coexpression levels of cAMP/PKA and PKA/CREB in the ACC of pain memory model rats, but indomethacin failed. The present findings identified a critical role of PKA in ACC in retrieval of pain memory. We propose that the proper mechanism of EA on pain memory is possibly due to the partial inhibition of cAMP/PKA/CREB signaling pathway by EA.
Does treating Alzheimer's disease early, delay institutionalisation?

Birmes P, Brunet A, Benoit M, et al. 2005. Validation of the Peritraumatic Dissociative Experiences Questionnaire self-report version in two samples of French-speaking individuals exposed to trauma. Eur Psychiatry 20: 145–151. Blake DD, Weathers FW, Nagy LM, et al. 1995. The development of a Clinician-Administered PTSD Scale. J Trauma Stress 8: 75–90. Brunet A, Weiss DS, Metzler TJ, et al. 2001. The Peritraumatic Distress Inventory: a proposed measure of PTSD criterion A2. Am J Psychiatry 158: 1480–1485. Chung MC, McKee KJ, Austin C, et al. 2009. Posttraumatic stress disorder in older people after a fall. Int J Geriatr Psychiatry 24: 955–964. Lensvelt-Mulders G, van der Hart O, van Ochten JM, et al. 2008. Relations among peritraumatic dissociation and posttraumatic stress: a metaanalysis. Clin Psychol Rev 28: 1138–1151.
Comparing the Effects of Traditional vs. Misconceptions-Based Instruction on Student Understanding of the Greenhouse Effect

ABSTRACT Conceptual change theory holds that pre-existing misconceptions persist under regular instruction and interfere with student acquisition of correct concepts. This implies that effective instruction should address both concepts and common misconceptions. Here, we investigate the efficacy of incorporating corrective information regarding well-documented misconceptions on the greenhouse effect and how the climate is affected by human activities into instruction on the greenhouse effect. Specifically, we compare student acquisition of fundamental concepts related to the greenhouse effect and climate change via traditional reading-based instruction (TRI) to misconceptions reading-based instruction (MRI). Lab sections from a first year Atmospheric Sciences course at a large public university were assigned to either a TRI or MRI treatment group, yielding a total of 197 subjects who successfully completed a pretest, the treatment, an immediate post-test, and a 2-week delayed post-test. Repeated-measures analysis of variance (ANOVA) with contrasts reveals that while both treatments produced significant gains in the post-test and delayed post-test overall, only the MRI treatment produced long-term gains on misconception-related questions. These results support the conceptual change model's claims that misconceptions may persist through concept-based instruction, but may be uprooted by instruction that addresses them directly. Based on these findings, it appears that a relatively brief reading passage focused on the basic science underlying a complex phenomenon, such as the greenhouse effect and human impact on climate, can be effective in improving student understanding. In addition, specifically addressing and instructing against misconceptions may improve learner acquisition of key concepts on which misconceptions exist.
Depletion of the Signal Recognition Particle Receptor Inactivates Ribosomes in Escherichia coli

ABSTRACT The signal recognition particle (SRP)-dependent cotranslational targeting of proteins to the cytoplasmic membrane in bacteria or the endoplasmic reticulum membrane in eukaryotes is an essential process in most living organisms. Eukaryotic cells have been shown to respond to an impairment of the SRP pathway by (i) repressing ribosome biogenesis, resulting in decreased protein synthesis, and (ii) by increasing the expression of protein quality control mechanisms, such as chaperones and proteases. In the current study, we have analyzed how bacteria like Escherichia coli respond to a gradual depletion of FtsY, the bacterial SRP receptor. Our analyses using cell-free transcription/translation systems showed that FtsY depletion inhibits the translation of both SRP-dependent and SRP-independent proteins. This synthesis defect is the result of a multifaceted response that includes the upregulation of the ribosome-inactivating protein ribosome modulation factor (RMF). Although the consequences of these responses in E. coli are very similar to some of the effects also observed in eukaryotic cells, one striking difference is that E. coli obviously does not reduce the rate of protein synthesis by downregulating ribosome biogenesis. Instead, the upregulation of RMF leads to a direct and reversible inhibition of translation.
Kenyan civil society: bridging the urban-rural divide?

While African civil society seemed a beacon of hope for democracy in the early 1990s, by the end of the decade many scholars had come to view it as extremely weak, lacking a domestic constituency and therefore any significant political or civic impact. Critics have been particularly concerned about urban-based 'democracy and governance' NGOs' limited influence on and connection with the rural majority of the citizenry. This article examines this question in Kenya, looking at four NGOs that have used civic education and paralegal programmes to establish a rural presence. Based on a survey of participants, it concludes that although the programmes are relatively new, they have begun to have a measurable impact on citizen understanding of politics, and have given the NGOs a noticeable rural presence. They have done so, however, by relying on ethnic, clan, partisan and other 'non-civil' networks to build supporters.
iSPY: Detecting IP Prefix Hijacking on My Own

IP prefix hijacking remains a major threat to the security of the Internet routing system due to a lack of authoritative prefix ownership information. Despite many efforts in designing IP prefix hijack detection schemes, no existing design can satisfy all the critical requirements of a truly effective system: real-time, accurate, lightweight, easily and incrementally deployable, as well as robust in victim notification. In this paper, we present a novel approach that fulfills all these goals by monitoring network reachability from key external transit networks to one's own network through lightweight prefix-owner-based active probing. Using the prefix-owner's view of reachability, our detection system, iSPY, can differentiate between IP prefix hijacking and network failures based on the observation that hijacking is likely to result in topologically more diverse polluted networks and unreachability. Through detailed simulations of Internet routing, 25-day deployment in 88 autonomous systems (ASs) (108 prefixes), and experiments with hijacking events of our own prefix from multiple locations, we demonstrate that iSPY is accurate with false negative ratio below 0.45% and false positive ratio below 0.17%. Furthermore, iSPY is truly real-time; it can detect hijacking events within a few minutes.
Syllable structure and syllable-related processes in German

Over the past few decades, the book series Linguistische Arbeiten [Linguistic Studies], comprising over 500 volumes, has made a significant contribution to the development of linguistic theory both in Germany and internationally. The series will continue to deliver new impulses for research and maintain the central insight of linguistics that progress can only be made in acquiring new knowledge about human languages both synchronically and diachronically by closely combining empirical and theoretical analyses. To this end, we invite submission of high-quality linguistic studies from all the central areas of general linguistics and the linguistics of individual languages which address topical questions, discuss new data and advance the development of linguistic theory.
On the dependence between base stations deployment and traffic spatial distribution in cellular networks

Base stations (BSs) deployment and traffic spatial distribution play crucial roles in network design and resource management. Actually, the BSs deployment and traffic spatial distribution are dually coupled, since BSs are built up to fulfill the traffic demand while data traffic is transmitted to mobile users through BSs. In this paper, basing on large-scale measurement data, we firstly show that both BSs deployment and traffic spatial distribution better follow an α-Stable distribution. Specifically, we study the statistical relationship between BSs density and traffic spatial density, which exhibits strong linear dependence for each cellular network generation. Furthermore, according to the slope parameter embedded in the linear regression model, we make a bold but reasonable assumption about the real network configuration evolution pattern, which is concretely manifested by the real transition from 2G to 3G and even 4G.
Annexin II and bleeding in acute promyelocytic leukemia.

BACKGROUND Acute promyelocytic leukemia (APL) is associated with a hemorrhagic disorder of unknown cause that responds to treatment with all-trans-retinoic acid. METHODS We studied a newly described receptor for fibrinolytic proteins, annexin II, in cells from patients with APL or other leukemias. We examined initial rates of in vitro generation of plasmin by tissue plasminogen activator (t-PA) in the presence of APL cells that did or did not have the characteristic translocation of APL, t(15;17). We also determined the effect of all-trans-retinoic acid on the expression of annexin II and the generation of cell-surface plasmin. RESULTS The expression of annexin II, as detected by a fluorescein-tagged antibody, was greater on leukemic cells from patients with APL than on other types of leukemic cells (mean fluorescence intensity, 6.9 and 2.9, respectively; P<0.01). The t(15;17)-positive APL cells stimulated the generation of cell-surface, t-PA-dependent plasmin twice as efficiently as the t(15;17)-negative cells. This increase in plasmin was blocked by an anti-annexin II antibody and was induced by transfection of t(15;17)-negative cells with annexin II complementary DNA. The t(15;17)-positive APL cells contained abundant messenger RNA for annexin II, which disappeared through a transcriptional mechanism after treatment with all-trans-retinoic acid. CONCLUSIONS Abnormally high levels of expression of annexin II on APL cells increase the production of plasmin, a fibrinolytic protein. Overexpression of annexin II may be a mechanism for the hemorrhagic complications of APL.