Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Prostaglandin H2 on Perinatal Pulmonary Circulation

ABSTRACT. A pivotal intermediate in prostaglandin (PG) biosynthesis is the endoperoxide PGH2. This endoperoxide is capable of eliciting direct responses in biological systems without undergoing conversion to other PGs. Effects of PGH2 include stimulation of platelet aggregation and vascular smooth muscle contraction in vitro; injections of PGH2 in vivo cause increases in pulmonary arterial pressure. The response of the pulmonary vasculature of perinatal lambs to PGH2 was measured using an in situ pumpperfused left lower lung preparation. Intrapulmonary injections of PGH2 (0.24-0.61 μg/kg) into six unventilated fetal lambs (0.93-0.97 gestation) produced decreases in pulmonary vascular resistance (PVR) of 10-21%. The fall in PVR was rapid in onset, reached a peak at 10 s after injection, and returned to baseline within 35 s. Following ventilation (FIO2=0.21) of fetal lambs, injections of PGH2 (0.24-0.61 μg/kg) caused increases in PVR (ave increase=50% over control PVR). The pulmonary pressor re-sponse to PGH2 in ventilated fetal lambs was depressed almost 50% by inhibition of thromboxane synthetase. Injections of a “heat-inactivated” PGH2 did not affect PVR in ventilated fetuses. We did not observe any effects on systemic blood pressure or heart rate of intrapulmonary arterial injections of PGH2. These findings suggest a me-tabolism of PGH2 to dilator PGs before ventilation and constrictor PGs and thromboxanes after ventilation, and or direct effects of PGH2 on vascular smooth muscle that are dependent on existing vascular tone.
Hyaluronic acid hydrogel loaded by adipose stem cells enhances wound healing by modulating IL-1β, TGF-β1, and bFGF in burn wound model in rat.

Application of hydrogels can be an effective technique in transferring the adipose-derived stem cells (ASCs) to injured tissue and their protection from further complications. Besides, acellular dermal matrix (ADM) has successfully been used in treatment of wounds. In this study, a combination of hylauronic acid (HA) and ASCs (HA/ASCs) was applied on burn wounds and the injured area was then covered by an ADM dressing in a rat model (ADM-HA/ASCs). Wound healing was evaluated by histopathological, histomorphometrical, molecular, biochemical, and scanning electron microscopy assessments on days 7, 14, and 28 post-wounding. ADM-HA/ASCs stimulated healing significantly more than the ADM-HA and ADM treated wounds, as it led to reduced inflammation, and improved angiogenesis and enhanced granulation tissue formation. Expression of interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) was lower in the ADM-HA/ASCs treated wounds than the ADM-HA and ADM groups, at the seventh post-wounding day. ADM-HA/ASCs also enhanced the expression level of TGF-β1 mRNA at 14 day post-wounding that was parallel to the experimental data from histological and biochemical assessments and confirmed the positive role of ASCs in repair of burn wounds. Additionally, increase in basic fibroblast growth factor (bFGF) expression and decreased TGF-β1 level on the 28th post-wounding day indicated the anti-scarring activity of ASCs. HA loaded by adipose stem cells can represent a promising strategy in accelerating burn wound healing.
National Surveillance of Methicillin-Resistant Staphylococcus aureus in China Highlights a Still-Evolving Epidemiology with 15 Novel Emerging Multilocus Sequence Types

ABSTRACT The global spread of methicillin-resistant Staphylococcus aureus (MRSA) is a serious problem, particularly in mainland China. In order to better understand the national molecular epidemiology and resistance profiles of hospital-associated MRSA (HA-MRSA) in China, a laboratory-based multicenter surveillance study was conducted. Sixty-nine hospitals in 45 large cities in 27 provinces were involved, and a total of 1,141 HA-MRSA isolates were collected during the 6-month study period in 2011. All MRSA isolates were characterized by multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) typing, spa typing, detection of the Panton-Valentine leukocidin (PVL) locus (lukS-PV and lukF-PV), and antibiogram analysis. ST239-III-t030, ST239-III-t037, and ST5-II-t002 were the predominant HA-MRSA clones (overall prevalence rates, 57.1%, 12.9%, and 8.1%, respectively), although the prevalence rates of these major clones varied markedly in different administrative regions. Of note, 6.6% of the HA-MRSA isolates were found to belong to ST59, which had typical community-associated MRSA (CA-MRSA) features, including carriage of SCCmec type IV or V and PVL and less antimicrobial resistance than other major HA-MRSA clones. Moreover, among 36 MLST sequence types (STs) identified, 15 STs, accounting for 3.5% of total isolates, were novel. A novel ST designated ST2590, which is a single-locus variant of ST5-II-t002, was identified in three hospitals in two large cities, with a total of 17 isolates. To further monitor trends in HA-MRSA prevalence, epidemic clonal shifts, clone emergence, and transmission between community and health care settings, longitudinal national MRSA surveillance is required.
Deubiquitinating c-Myc: USP36 steps up in the nucleolus

Ubiquitination plays a key and complex role in the regulation of c-Myc stability, transactivation, and oncogenic activity. c-Myc is ubiquitinated by a number of ubiquitin ligases (E3s), such as SCFFbw7 and SCFSkp2. Depending on the E3s, ubiquitination can either positively or negatively regulate c-Myc levels and activity. Meanwhile, c-Myc ubiquitination can be reversed by deubiquitination. An early study showed that USP28 deubiquitinates c-Myc via interacting with Fbw7α whereas a recent study reveals that USP37 deubiquitinates c-Myc independently of Fbw7 and c-Myc phosphorylation. Consequently, both USP28 and USP37 stabilize c-Myc and enhance its activity. We recently found the nucleolar USP36 as a novel c-Myc deubiquitinase that controls the end-point of c-Myc degradation pathway in the nucleolus. Here we briefly review the current understanding of ubiquitination and deubiquitination regulation of c-Myc and further discuss the USP36-c-Myc regulatory pathway.
Transfer of Expert Visual Anticipation to a Similar Domain

The experiment reported in this paper examined the capability of expert and near-expert baseball batters and novices to transfer anticipatory skill to a cricket batting prediction task. A video-simulation temporal occlusion paradigm was used to first assess the anticipatory skill of expertise groups in a baseball batting prediction task (learning sport) and, second, to assess the capability of expertise groups to transfer anticipation skill to a cricket batting prediction task (transfer sport). Results showed that expert and near-expert baseball batters were superior to novices at anticipating pitch type based upon pre-ball-flight advance information. Only expert baseball batters were capable of transferring their anticipatory skill to predict delivery type based upon advance information in the bowler's action, whilst near-experts and novices relied upon ball-flight information. The findings extend understanding of transfer of learning in the motor domain and some theoretical/empirical concepts of transfer.
Microwave Emission and Scattering From Deserts: Theory Compared With Satellite Measurements

The emission and scattering from desert surfaces are analyzed using simulations and measurements from the Special Sensor Microwave/Imager (SSM/I) and the Advanced Microwave Sounding Unit (AMSU) microwave satellite instruments. Deserts are virtually free of vegetation, so the satellite radiometers are able to observe the emissivities of different minerals, such as limestone and quartz. Moreover, since deserts contain little moisture, the thermal emission originates below the surface at a depth of many wavelengths. At high frequencies, where the penetration depth of radiation is smallest, the radiometric measurements display the large diurnal variation in surface temperature, which reaches its maximum at around 1 P.M. Conversely, at low frequencies, where the penetration depth is largest, the radiation measurements display the small diurnal variation of subsurface temperature, which reaches a minimum at around 6 A.M. In addition to these emission signals, sand particles also scatter microwave radiation. Volume scattering causes the measurements to decrease as the frequency increases; although compared to other scattering media (snow cover and precipitation), the larger absorption and fractional volume (i.e., solidity) of sand reduce the scattering. Although the scattering effect is small, SSM/I measurements between 19 and 85 GHz show that deserts scatter the upwelling microwave radiation in a manner similar to light precipitation, which makes it difficult to uniquely identify precipitation over arid regions. Interestingly, the higher frequency AMSU measurement at 150 GHz is nearly the same as at 89 GHz for deserts, whereas the 150-GHz measurement is much lower than at 89 GHz for precipitation. These different spectral features at high frequencies can provide a means of separating the scattering from desert surfaces from that of precipitation.
Economic Profiling of Physician Specialists: Use of Outlier Treatment and Episode Attribution Rules

This paper examines the influence of episode attribution methodology and cost outlier methodology on the accuracy of physicians' economic profiles. Four years of claims data from a mixed model HMO were processed using the leading episode grouper software. Episode grouped results then were applied to construct input distributions for a simulation model. For each of four specialties (cardiology, family practice, general surgery, and neurology), we employed sets of 18 simulations to investigate the effects of three alternative episode attribution methodologies and six alternative cost outlier methodologies on sensitivity, specificity, and positive predictive error in classifying cost-efficient and cost-inefficient physicians. For identification of cost-efficient physicians, the most accurate profiling results were obtained when Winsorizing outliers at 2% and 98% of episode-type cost distributions, and attributing responsibility for episode costs to physicians who accounted for at least 30% of associated professional and prescribing fees. No consistent combination of outlier methodology and episode attribution rule was found to be superior for identifying cost-inefficient physicians.
Efficiency and ergonomic benefits of using radio controlled chokers in cable yarding.

Using radio controlled chokers in cable yarding can improve both productivity and safety during the unhooking phase part of the extraction cycle. However, the additional weight of the radio controlled chokers may also increase the work load of the choker-setters on the slope. A study has been completed in Austria on the Wanderfalke yarder. The factorial study design alternated extraction corridors with and without the use of radio controlled chokers. A standard choker for this system weighs 0.34 kg and cost 11 Euro each, whereas the radio controlled chokers weighs 1.6 kg and cost 9,000 Euros for the set of four chokers. Work-load was measured by continuously monitoring the heart-rate of the workers. Results showed that was a slight productivity gain, but also an increased work-load for the choker-setter. This is reflected in the comments from the yarder crew; the yarder operator thinks it great not having to get down to unhook the turn, the choker-setter is not impressed with the extra weight and work load.
The immunological mechanisms that control pneumococcal carriage

Colonization of the human nasopharynx by pneumococcus is extremely common and is both the primary reservoir for transmission and a prerequisite for disease. Current vaccines targeting the polysaccharide capsule effectively prevent colonization, conferring herd protection within vaccinated communities. However, these vaccines cover only a subset of all circulating pneumococcal strains, and serotype replacement has been observed. Given the success of pneumococcal conjugate vaccine (PCV) in preventing colonization in unvaccinated adults within vaccinated communities, reducing nasopharyngeal colonization has become an outcome of interest for novel vaccines. Here, we discuss the immunological mechanisms that control nasopharyngeal colonization, with an emphasis on findings from human studies. Increased understanding of these immunological mechanisms is required to identify correlates of protection against colonization that will facilitate the early testing and design of novel vaccines.
Monitoring and evaluation of information and communication technologies (ICT) in education projects : a handbook for developing countries

The increasing profile and importance of the use of information and communication technologies (ICTs) in the education sector are visible in many developing countries. This paper includes the following content: chapter 1. monitoring and evaluation of ICT for education: an introduction; chapter 2. monitoring and evaluation of ICT for education impact: a review; chapter 3. core indicators for monitoring and evaluation studies for ICT in education; chapter 4. developing a monitoring and evaluation plan for ICT in education; chapter 5. capacity building and management in ICT for education; chapter 6. pro-equity approaches to monitoring and evaluation - gender, marginalized groups and special needs populations; and chapter 7. do's and don'ts in monitoring and evaluation.
An analysis of dynamic forces transmitted through the foot in diabetic neuropathy.

OBJECTIVE Biomechanical studies in diabetic neuropathy have clearly demonstrated abnormal foot pressures, but information on other aspects of gait is limited. This study aimed to investigate and describe the forces transmitted through the foot during walking in diabetic subjects with varying degrees of peripheral neuropathy and to determine if abnormalities in these forces might contribute to the risk of plantar ulceration. RESEARCH DESIGN AND METHODS Subjects from the following groups were included: healthy control subjects (C); diabetic control subjects (D); subjects with diabetic neuropathy (DN); subjects with previous neuropathic ulceration (DNU); and subjects with Charcot neuro-arthropathy (CH). Gait analysis was performed as subjects walked over a Kistler force plate. Peak forces were measured (as percent body weight) in the vertical and horizontal planes. Comparisons were made between all of the groups and between each diabetic group and a healthy control group matched for walking speed. RESULTS There were 181 subjects studied. In comparison with that of the speed-matched controls, the mean peak vertical force was higher in each of the diabetic groups, especially in the most neuropathic subjects (DNU, 113 vs. 110%, P < 0.01). This increase was entirely due to higher forces during heel contact (DNU, 111 vs. 106%, P < 0.001). The single peak force occurred during heel strike (rather than during foot push-off) in 23-38% of footsteps of healthy and diabetic control subjects but in 53-73% of footsteps of neuropathic subjects. There was also a trend for higher peak medial forces (CH, 6.2 vs. 5.5%, P < 0.05). CONCLUSIONS Diabetic neuropathy is associated with a change in the time pattern of the forces transmitted through the foot and an increase in the vertical forces through the heel. The magnitude of the changes is small in absolute terms, but these changes may contribute to the risk of plantar foot ulceration.
An Economic Assessment of Tax Policy in the Bush Administration, 2001-2004

: This Article analyzes the economic effects of the George W. Bush administration's tax policies. It describes the 2001, 2002, and 2003 tax cuts and the proposals to make them permanent, and then explores the consequences of making the tax cuts permanent on the fiscal status of the government, the distribution of after-tax income, long-term economic growth, and the prospects for fundamental tax reform. This article also examines the role of the tax cuts as a short-term stimulus over the past few years.
Cofilin 1-Mediated Biphasic F-Actin Dynamics of Neuronal Cells Affect Herpes Simplex Virus 1 Infection and Replication

ABSTRACT Herpes simplex virus 1 (HSV-1) invades the nervous system and causes pathological changes. In this study, we defined the remodeling of F-actin and its possible mechanisms during HSV-1 infection of neuronal cells. HSV-1 infection enhanced the formation of F-actin-based structures in the early stage of infection, which was followed by a continuous decrease in F-actin during the later stages of infection. The disruption of F-actin dynamics by chemical inhibitors significantly reduced the efficiency of viral infection and intracellular HSV-1 replication. The active form of the actin-depolymerizing factor cofilin 1 was found to increase at an early stage of infection and then to continuously decrease in a manner that corresponded to the remodeling pattern of F-actin, suggesting that cofilin 1 may be involved in the biphasic F-actin dynamics induced by HSV-1 infection. Knockdown of cofilin 1 impaired HSV-1-induced F-actin assembly during early infection and inhibited viral entry; however, overexpression of cofilin 1 did not affect F-actin assembly or viral entry during early infection but decreased intracellular viral reproduction efficiently. Our results, for the first time, demonstrated the biphasic F-actin dynamics in HSV-1 neuronal infection and confirmed the association of F-actin with the changes in the expression and activity of cofilin 1. These results may provide insight into the mechanism by which HSV-1 productively infects neuronal cells and causes pathogenesis.
Phylogenetic studies of four Anser cygnoides (Anserini: Anserinae) in Hunan province of China based on complete mitochondrial DNA sequences

Abstract In this study, we cloned and sequenced the complete mitochondrial DNAs of Chinese goose, Anser cygnoides populations from three different areas of Hunan province in China. The Anser cygnoides breed Wugangtong white goose (WGTW) sample and Wugangtong grey goose sample (WGTG) were taken from the Wugang county of Shaoyang city, the Anser cygnoides breed Xupu goose (XP) sample was taken from the Xupu county of Huaihua city, and the Anser cygnoides breed Yanling white goose (YLW) sample was taken from the Yanling county of Zhuzhou city. The organization of the four Anser cygnoides breeds mitochondrial genomes was similar. Phylogenetic analyses using N-J computational algorithms showed that the analyzed species are divided into four major clades: Anatinae, Anserinae, Dendrocygninae and Anseranatidae. It was noted that Wugangtong white goose, Yanling white goose and Xupu goose have highly similar phylogenetic relationship.
The European External Action Service: Torn Apart Between Several Principals or Acting as a Smart ‘Double-agent’?

The European External Action Service (EEAS) is a hybrid and compound institutional actor in the EU’s multi-level administration with delegated authority from the member states (MS) to conduct the EU’s external action. Substantial competences, notably in the field of Neighbourhood and Trade policies, as well as Development and Cooperation remain under the control of the European Commission (Commission). At the same time, also Members of the European Parliament (EP) are more clearly voicing their interests and ownership in the EU’s representation in the world. This article tests the notion of 'double-agent' – or in fact “triple-agent” – as a way of characterizing the position of the EEAS, and in particular of the EU Delegations (as the ‘EU field-level bureaucracies’) vis-à-vis the MS, the Commission and the EP, as an expression of complex and interrelated chains of delegation, where the EU ‘embassies‘ have to interact with and to answer to (but not in a clear line of delegation) different (sets of) principals, namely the MS, the Commission and the EP. Based on the findings from a series of elite interviews with 47 EEAS and Commission officials and on a survey among 184 EU diplomats, the paper seeks to examine this fuzzy principal-agent relationship and uses the review process of the EEAS as an opportunity to assess the level of autonomy of the new EU foreign policy apparatus.
Tumor Heterogeneity in Pancreatic Adenocarcinoma

Pancreatic adenocarcinoma is one of the deadliest malignancies worldwide, mainly due to frequent diagnosis at an advanced stage and its strong chemoresistance. Tumor heterogeneity is evident at the histological level, both between tumors and even within a tumor. Recent high-throughput analyses have confirmed that intertumor heterogeneity is greater than intratumor heterogeneity that is mostly driven by successive catastrophic genetic events in the early stage and by epigenetic modifications in the metastatic stage. While this heterogeneity may complicate the search for a universal cure, these analyses have distinguished several subtypes at the genomic, transcriptomic, and metabolomic levels that offer, for some, new therapeutic opportunities.
On the Public-Private School Achievement Debate

On July 14, 2006, the U. S. Department of Education’s National Center for Education Statistics (NCES) released a study that compared the performance in reading and math of 4th and 8th-graders attending private and public schools. Using information from a nationwide, representative sample of public and private school students collected in 2003 as part of the ongoing National Assessment of Educational Progress (NAEP), the NCES study reported that the performance of students attending private schools was superior to that of students attending public schools. But after statistical adjustments were made for student characteristics, the private school advantage among 4th-graders was reported to give way to a 4.5-point public school advantage in math and school-sector parity in reading. After the same adjustments were made for 8th-graders, private schools retained a 7-point advantage in reading but achieved only parity in math. In this paper, we argue that NCES’s measures of student characteristics were flawed by inconsistent classification across the public and private sectors and by the inclusion of factors open to school influence. Utilizing the same data as the original study but substituting better measures of student characteristics, improved, alternative models identify a private school advantage in 11 out of 12 public-private comparisons. In 8th-grade math, the private school advantage varies between 3 and 6.5 test points; in reading, it varies between 9 and 12.5 points. Among 4th graders, in math, parity is observed in one model, but private schools outperform public schools by 2 and 3 points in the other two models; in 4th-grade reading, private schools have an advantage that ranges from 7 to 10 points. However, although the alternative models constitute an improvement on the NCES model, no conclusions should be drawn as to causal relationships from these or any other results based on NAEP test scores, because they are too fragile to be used for such purposes. Inferring causality from observations at one point in time is highly problematic.
Opioid analgesic dose and the risk of misuse, overdose, and death: A narrative review

Despite the rise in serious adverse events paralleling increased prescription opioid analgesic use in the United States over the past 2 decades, the association between opioid analgesic dose and the risk of serious adverse health outcomes is incompletely characterized. We sought to synthesize the medical literature for observational studies examining the association between opioid analgesic dose and the risk of serious adverse health outcomes, with particular attention to the outcomes of misuse, abuse, addiction, overdose, and death.
Improving analysis of the size and dynamics of AIDS epidemics

Supplement editors Peter D Ghys and Neff Walker and the Chair of the UNAIDS Reference Group on Estimates, Modelling and Projections, Geoff P Garnett, introduce 13 papers describing the data, methods, and tools used to produce the 2005 UNAIDS/WHO HIV and AIDS estimates