Miracle-dz/UniFAR-FTUs huggingface dataset:
Perceived Concerns of Patients at Risk for Lower Extremity Amputation.

Despite a good understanding of risk factors for amputation and the development of multidisciplinary amputation prevention teams, thousands of lower limb amputations are performed on a daily basis. These amputations are often transformative events in the lives of patients with functional, psychological, social, and economic implications. OBJECTIVE The objective of this investigation is to qualitatively and quantitatively explore the perceived concerns of patients with chronic wounds at risk for lower extremity amputation. MATERIALS AND METHODS A guided, physician-administered survey was completed by consenting participants. The survey consisted of both open-ended questions and a 10-point scale for specific questions on a variety of potential patient concerns. RESULTS Although some questions resulted in relatively high and low mean scores, 9 of the 13 specific questions produced a range of responses on a 10-point modified Likert scale. This indicates that there are not necessarily universal patient concerns and that every patient is different and should be treated as such. With that being said, however, the highest levels of concern (mean measurements ≥ 7/10) had to do with recurrence, function, walking, and self-sufficiency. The lowest levels of concern (mean measurement ≤ 5/10) had to do with pain, shoe gear considerations, cost, and cosmetic appearance of an amputation. CONCLUSIONS It is the authors' hope that this investigation provides wound care professionals with information that will benefit their approach to the education and treatment of patients at risk for amputation as well as lead to future investigations into the emotional and psychological state of patients with chronic lower extremity wounds.
Identification of a novel gene in ROD9 island of Salmonella Enteritidis involved in the alteration of virulence-associated genes expression

ABSTRACT Salmonella enterica subsp. I serovar Enteritidis (S. Enteritidis), one of the causative agents for non-typhoidal gastrointestinal diseases in humans is an intracellular bacterium and mechanism for its invasion into host cells is critical to cause infection. The virulence of the pathogen is explained by the expression of genes located on its pathogenicity islands, mostly encoded under SPI-1 and SPI-2. However, S. Typhimurium SL1344, despite sharing ∼98% of its genome with S. Enteritidis P125109, lacks few regions of differences (ROD) that are hypothesized to impart virulence potential to S. Enteritidis. In this study, we created different mutants in the ROD9 island of S. Enteritidis, also referred as SPI-19 and identified a novel locus, SEN1005, encoding a hypothetical protein that is involved in its pathogenesis. ΔSEN1005 displayed significantly reduced entry into cultured epithelial cells as well as uptake by macrophages and failed to cause acute colitis in C57BL/6 mice at day 3 post-infection (p.i.). Additionally, the global transcriptome analysis revealed a highly repressed SPI-1 and other down-regulated genes responsible for flagellar assembly, chemotaxis and motility in the mutant which correlated with decreased invasion and abated inflammation as compared to the wild-type. Therefore, our findings revealed that ΔSEN1005 was attenuated in vitro as well as in vivo and we propose this hypothetical protein to play a role in altering the expression of genes involved in Salmonella virulence.
Electrophysiological and Morphological Characterization of Chrna2 Cells in the Subiculum and CA1 of the Hippocampus: An Optogenetic Investigation

The nicotinic acetylcholine receptor alpha2 subunit (Chrna2) is a specific marker for oriens lacunosum-moleculare (OLM) interneurons in the dorsal CA1 region of the hippocampus. It was recently shown using a Chrna2-cre mice line that OLM interneurons can modulate entorhinal cortex and CA3 inputs and may therefore have an important role in gating, encoding, and recall of memory. In this study, we have used a combination of electrophysiology and optogenetics using Chrna2-cre mice to determine the role of Chrna2 interneurons in the subiculum area, the main output region of the hippocampus. We aimed to assess the similarities between Chrna2 subiculum and CA1 neurons in terms of the expression of interneuron markers, their membrane properties, and their inhibitory input to pyramidal neurons. We found that subiculum and CA1 dorsal Chrna2 cells similarly expressed the marker somatostatin and had comparable membrane and firing properties. The somas of Chrna2 cells in both regions were found in the deepest layer with axons projecting superficially. However, subiculum Chrna2 cells displayed more extensive projections with dendrites which occupied a significantly larger area than in CA1. The post-synaptic responses elicited by Chrna2 cells in pyramidal cells of both regions revealed comparable inhibitory responses elicited by GABAA receptors and, interestingly, GABAB receptor mediated components. This study provides the first in-depth characterization of Chrna2 cells in the subiculum, and suggests that subiculum and CA1 Chrna2 cells are generally similar and may play comparable roles in both sub-regions.
A comparative study of kinematic and acoustic age- related variability in speech

In this study, we compared age-related lip movement variability with acoustic variability across repetitions using Functional Data Analysis (FDA). Lip movement and acoustic data of 1520 repetitions of a short Swedish phrase from 50 Swedish speakers were collected. Results showed weak to moderate negative correlations for age and variability in lip movements, phrase duration and sound pressure level, which confirm previous studies of decreasing variability in speech with age. Variability in F 0 did not vary with age, and this may be because we tend to vary our intonation in speech both unconciously when we are learning to speak, and conciously as adult speakers. This will be explored further in a follow-up study.
Cladogenetic and Anagenetic Models of Chromosome Number Evolution: a Bayesian Model Averaging Approach

Chromosome number is a key feature of the higher-order organization of the genome, and changes in chromosome number play a fundamental role in evolution. Dysploid gains and losses in chromosome number, as well as polyploidization events, may drive reproductive isolation and lineage diversification. The recent development of probabilistic models of chromosome number evolution in the groundbreaking work by Mayrose et al. (2010, ChromEvol) have enabled the inference of ancestral chromosome numbers over molecular phylogenies and generated new interest in studying the role of chromosome changes in evolution. However, the ChromEvol approach assumes all changes occur anagenetically (along branches), and does not model events that are specifically cladogenetic. Cladogenetic changes may be expected if chromosome changes result in reproductive isolation. Here we present a new class of models of chromosome number evolution (called ChromoSSE) that incorporate both anagenetic and cladogenetic change. The ChromoSSE models allow us to determine the mode of chromosome number evolution; is chromosome evolution occurring primarily within lineages, primarily at lineage splitting, or in clade-specific combinations of both? Furthermore, we can estimate the location and timing of possible chromosome speciation events over the phylogeny. We implemented ChromoSSE in a Bayesian statistical framework, specifically in the software RevBayes, to accommodate uncertainty in parameter estimates while leveraging the full power of likelihood based methods. We tested ChromoSSE’s accuracy with simulations and re-examined chromosomal evolution in Aristolochia, Carex section Spirostachyae, Helianthus, Mimulus sensu lato (s.l.), and Primula section Aleuritia, finding evidence for clade-specific combinations of anagenetic and cladogenetic dysploid and polyploid modes of chromosome evolution.
Closed-Loop Targeted Memory Reactivation during Sleep Improves Spatial Navigation

Sounds associated with newly learned information that are replayed during non-rapid eye movement (NREM) sleep can improve recall in simple tasks. The mechanism for this improvement is presumed to be reactivation of the newly learned memory during sleep when consolidation takes place. We have developed an EEG-based closed-loop system to precisely deliver sensory stimulation at the time of down-state to up-state transitions during NREM sleep. Here, we demonstrate that applying this technology to participants performing a realistic navigation task in virtual reality results in a significant improvement in navigation efficiency after sleep that is accompanied by increases in the spectral power especially in the fast (12–15 Hz) sleep spindle band. Our results show promise for the application of sleep-based interventions to drive improvement in real-world tasks.
Sporulation capability and amylosome conservation among diverse human colonic and rumen isolates of the keystone starch‐degrader Ruminococcus bromii

Summary Ruminococcus bromii is a dominant member of the human colonic microbiota that plays a ‘keystone’ role in degrading dietary resistant starch. Recent evidence from one strain has uncovered a unique cell surface ‘amylosome’ complex that organizes starch‐degrading enzymes. New genome analysis presented here reveals further features of this complex and shows remarkable conservation of amylosome components between human colonic strains from three different continents and a R. bromii strain from the rumen of Australian cattle. These R. bromii strains encode a narrow spectrum of carbohydrate active enzymes (CAZymes) that reflect extreme specialization in starch utilization. Starch hydrolysis products are taken up mainly as oligosaccharides, with only one strain able to grow on glucose. The human strains, but not the rumen strain, also possess transporters that allow growth on galactose and fructose. R. bromii strains possess a full complement of sporulation and spore germination genes and we demonstrate the ability to form spores that survive exposure to air. Spore formation is likely to be a critical factor in the ecology of this nutritionally highly specialized bacterium, which was previously regarded as ‘non‐sporing’, helping to explain its widespread occurrence in the gut microbiota through the ability to transmit between hosts.
Effectiveness of polyol blends as cryoprotectants in surimi and natural actomyosin from ling cod (ophiodon elongatus)

Cryoprotectants with decreased sweetness are desirable for products like surimi because of consumer preference for less sweet foods. Physical, biochemical and Raman spectroscopic techniques were used to evaluate such cryoprotectants in ling cod surimi and natural actomyosin (NAM) model systems. Cryoprotectant blends with decreased sweetness were first investigated for their potential to stabilize ling cod surimi during frozen storage at -18°C for 4 months. A central composite rotatable design (CCRD) was used to formulate 25 blends containing lactitol, LitesseTM, sucrose, and sorbitol, at final total concentrations of 4-12%. Although decreases in % salt extractable proteins, apparent viscosity and water binding capacity were observed, the gel strength, colour, pH and myosin heavy chain.actin ratio did not change significantly after frozen storage in surimi containing cryoprotectant blends. Differences for surimi with and without cryoprotectants were significant (P > 0.05). All 25 blends gave surimi and cooked gels comparable to the commercial blend (4% sucrose, 4%> sorbitol). For optimization of cryoprotectant blend composition, a CCRD was used to formulate 25 blends containing lactitol, LitesseTM, sucrose and sorbitol at final total concentrations of 2-6%>. Highly significant regression models (P<0.001) were obtained for gel strength, firmness, fold test, total and specific ATPase activities. Sensory evaluation indicated that the commercial blend was perceived to be sweeter than the 5.5 and 6%> optimal blends (P<0.05). Individual cryoprotectants at 8%, optimal blends at 4, 5.5, 6 and 8%) and the commercial blend were all effective in maintaining gel strength of N A M and surimi gels. Hydrogen, ionic and hydrophobic interactions were the main forces contributing to
Multi-Task Learning of Pairwise Sequence Classification Tasks over Disparate Label Spaces

We combine multi-task learning and semi-supervised learning by inducing a joint embedding space between disparate label spaces and learning transfer functions between label embeddings, enabling us to jointly leverage unlabelled data and auxiliary, annotated datasets. We evaluate our approach on a variety of tasks with disparate label spaces. We outperform strong single and multi-task baselines and achieve a new state of the art for aspect-based and topic-based sentiment analysis.
Hyperphosphatemia is associated with high mortality in severe burns

Introduction Phosphate level is often deranged during acute illness, regardless of the presence of kidney injury or not. A few studies described that hypophosphatemia may associated with outcome in patients admitted to the burn unit, but the literatures for hyperphosphatemia is limited. Our study aims to evaluate if hyperphosphatemia, one of the sign of severe tissue damage or kidney injury, will associate with mortality of patients with severe burns. Materials and methods The study was a post hoc analysis of prospectively collected data from patients admitted to the burn unit between September 2006 and December 2011. Patients were stratified into normophosphatemic or hyperphosphatemic group by baseline plasma phosphate level. The primary endpoint is 90-day mortality. Results Total 301 patients were included (hyperphosphatemia: n = 52; normophosphatemia: n = 249). The hyperphosphatemic group had lower Glasgow Coma Scale, mean arterial blood pressure, hemoglobin level, albumin, and higher TBSA of burns, APACHE II score, ABSI score, Acute kidney injury (AKI), and creatinine. The 90-day mortality was higher in the hyperphosphatemic group than in the normal group (53.8% vs 18.1%, P < .001) and this difference was still significant when adjusting for several confounding factors (hazard ratio, 2.05; 95% CI, 1.17–3.59). Multivariable Cox analysis showed risk factors of mortality included TBSA of burns, hyperphosphatemia, reduced urine output, and APACHE II score. Conclusions Our study found in addition to TBSA of burns and inhalation injury, baseline hyperphosphatemia in patients with severe burns is also associated with higher mortality.
Mutations of charged amino acids at the cytoplasmic end of transmembrane helix 2 affect transport activity of the budding yeast multidrug resistance protein Pdr5p.

Pdr5p is a major ATP-binding cassette (ABC) transporter in Saccharomyces cerevisiae. It displays a sequence and functional homology to the pathogenic Candida albicans multidrug resistance protein Cdr1p. The transmembrane helices of Pdr5p act in substrate recognition, binding, translocation and eventual removal of toxic substances out of the plasma membrane via the formation of a binding pocket. In this study, we identify two novel Pdr5 mutants (E574K and E580K), which exhibit impaired substrate efflux functions. Both mutants remained hypersensitive to all tested Pdr5p substrates without affecting their protein expression levels, localization or ATPase activities. As E574 and E580 are both located adjacent to the predicted cytoplasmic end of transmembrane helix 2, this implies that such charged residues are functionally essential for Pdr5p. Molecular docking studies suggest the possibility that oppositely charged substitution at residue E574 may disturb the interaction between the substrates and Pdr5p, resulting in impaired transport activity. Our results present new evidence, suggesting that transmembrane helix 2 plays an important role for the efflux function of Pdr5p.
Application of a Hybrid Model Based on a Convolutional Auto-Encoder and Convolutional Neural Network in Object-Oriented Remote Sensing Classification

Variation in the format and classification requirements for remote sensing data makes establishing a standard remote sensing sample dataset difficult. As a result, few remote sensing deep neural network models have been widely accepted. We propose a hybrid deep neural network model based on a convolutional auto-encoder and a complementary convolutional neural network to solve this problem. The convolutional auto-encoder supports feature extraction and data dimension reduction of remote sensing data. The extracted features are input into the convolutional neural network and subsequently classified. Experimental results show that in the proposed model, the classification accuracy increases from 0.916 to 0.944, compared to a traditional convolutional neural network model; furthermore, the number of training runs is reduced from 40,000 to 22,000, and the number of labelled samples can be reduced by more than half, all while ensuring a classification accuracy of no less than 0.9, which suggests the effectiveness and feasibility of the proposed model.
An RW inspired research trajectory : Modeling and ( Modular and Hierarchical ) Control Aspects

This note presents a local approach for the modular supervisory control of timed discrete-event systems. Our modular supervisors are designed over local models, which are obtained by aggregating only the subsystems affected by each control specification. We establish necessary and sufficient conditions for nonblocking optimal global behavior. We also present a comparative analysis of the computational advantages and limitations of the proposed strategy.
EFFECT OF BORAX ON MECHANICAL PROPERTIES AND BIODEGRADABILITY OF SAGO STARCH – POLY(VINYL ALCOHOL) BLEND FILMS

Biodegradable films of sago starch and poly(vinyl alcohol) (PVA) blends have been prepared by solution casting method. Glycerol was used as a plasticizer. Sodium tetraborate (borax) was used to increase the compatibility of sago starch and PVA. The optimum amount of glycerol is 20% (w/w). The optimum values of tensile strength and elongation at break of the films are obtained with an addition of 8% (w/w) borax. Both pure PVA and sago starch films are apparently transparent. However, their blend films are slightly opaque probably due to phase separation. Increase the amount of PVA in the film leads to an increase of the tensile strength and reduces the elongation of films. Borax increases the elongation at break of the sago starch – PVA blend films and also improves the tensile strength of the films. Biodegradation of original and modified sago starch – PVA blend films have been studied by soil burial method. Percentage of weight loss of SPB films increase with an increase of sago starch content. Keywords: biodegradable films, sago starch, poly(vinyl alcohol), sodium tetraborate
The Positive Effect of Resilience on Stress and Business Outcomes in Difficult Work Environments

Objective: To examine whether resilience has a protective effect in difficult work environments. Methods: A survey of 2063 individuals measured individual resilience, stress, burnout, sleep problems, likelihood of depression, job satisfaction, intent to quit, absences, and productivity. It also measured work characteristics: job demands, job influence, and social support. Multivariate and logistic regression models examined the main effects and interactions of resilience and job characteristics. Results: High strain work environments (high demand, low influence, and low support) have an unfavorable effect on all outcomes. Resilience has a protective effect on all outcomes. For stress, burnout, and sleep, higher resilience has a more protective effect under low-strain conditions. For depression, absence and productivity, resilience has a more protective effect when job strain is high. Conclusions: Workers with high resilience have better outcomes in difficult work environments.
Strategy combination during execution of memory strategies in young and older adults

ABSTRACT The present study investigated whether people can combine two memory strategies to encode pairs of words more efficiently than with a single strategy, and age-related differences in such strategy combination. Young and older adults were asked to encode pairs of words (e.g., satellite-tunnel). For each item, participants were told to use either the interactive-imagery strategy (e.g., mentally visualising the two words and making them interact), the sentence-generation strategy (i.e., generate a sentence linking the two words), or with strategy combination (i.e., generating a sentence while mentally visualising it). Participants obtained better recall performance on items encoded with strategy combination than on items encoded with interactive-imagery or sentence-generation strategies. Moreover, we found age-related decline in such strategy combination. These findings have important implications to further our understanding of execution of memory strategies, and suggest that strategy combination occurs in a variety of cognitive domains.
Assessing the Contributions of Local and East Pacific Warming to the 2015 Droughts in Ethiopia and Southern Africa

Introduction. In northern Ethiopia (7°–14°N, 36.5°– 40.5°E, NE) during June–September (JJAS) of 2015 and in southern Africa (13.5°–27°S, 26.5°–36°E, SA) during December–February (DJF) of 2015/16, main growing seasons rains were extremely poor. In Ethiopia, Climate Hazards Group Infrared Precipitation with Stations (CHIRPS) (Funk et al. 2015c) and Centennial Trends (Funk et al. 2015b) data indicated one of the worst droughts in more than 50 years (FEWSNET 2015). More than ten million people currently require humanitarian relief (FEWSNET 2016a). SA rains were also extremely poor (FEWSNET 2016b); in Mozambique and Malawi, February maize prices were more than twice the five-year average, and in Zimbabwe the president has declared a national disaster in view of the El Niño–induced poor rains and the escalating food insecurity situation. NE has been experiencing long-term rainfall declines (Funk et al. 2008; Funk et al. 2005; Jury and Funk 2013; Viste et al. 2012; Williams et al. 2012). The eastern Ethiopian highlands have exhibited recurrent soil moisture and runoff deficits since the 1990s (Funk et al. 2015c). NE rains in 2015 were the driest on record, but station data density prior to 1950 is very sparse for Ethiopia (Funk et al. 2015b). SA rainfall does not exhibit a decline, but the 2015–16 drought was severe. The impact of ENSO on Ethiopian rainfall is well documented (Fig. S15.1; Camberlin 1997; Degefu 1987; Diro et al. 2011; Gissila et al. 2004; Korecha and Barnston 2007; Korecha and Sorteberg 2013; Segele and Lamb 2005): the warm phase of ENSO is associated with suppressed rains during the main wet season (JJAS) over north and central Ethiopia. There have also been numerous papers documenting a negative teleconnection between El Niño and SA rainfall (Supplemental Fig. S15.1; Hoell et al. 2015; Jury et al. 1994; Lindesay 1988; Misra 2003; Nicholson and Entekhabi 1986; Nicholson and Kim 1997; Reason et al. 2000; Rocha and Simmonds 1997).