Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of aortic extracellular matrix synthesis via noradrenergic system and angiotensin II in juvenile rats

Context: Extracellular matrix (ECM) synthesis regulation by sympathetic nervous system (SNS) or angiotensin II (ANG II) was widely reported, but interaction between the two systems on ECM synthesis needs further investigation. Objective: We tested implication of SNS and ANG II on ECM synthesis in juvenile rat aorta. Materials and methods: Sympathectomy with guanethidine (50 mg/kg, subcutaneous) and blockade of the ANG II AT1 receptors (AT1R) blocker with losartan (20 mg/kg/day in drinking water) were performed alone or in combination in rats. mRNA and protein synthesis of collagen and elastin were examined by Q-RT-PCR and immunoblotting. Results: Collagen type I and III mRNA were increased respectively by 62 and 43% after sympathectomy and decreased respectively by 31 and 60% after AT1R blockade. Combined treatment increased collagen type III by 36% but not collagen type I. The same tendency of collagen expression was observed at mRNA and protein levels after the three treatments. mRNA and protein level of elastin was decreased respectively by 63 and 39% and increased by 158 and 15% after losartan treatment. Combined treatment abrogates changes induced by single treatments. Discussion and conclusion: The two systems act as antagonists on ECM expression in the aorta and combined inhibition of the two systems prevents imbalance of mRNA and protein level of collagen I and elastin induced by single treatment. Combined inhibition of the two systems prevents deposit or excessive reduction of ECM and can more prevent cardiovascular disorders.
Positive selection of a duplicated UV-sensitive visual pigment coincides with wing pigment evolution in Heliconius butterflies

The butterfly Heliconius erato can see from the UV to the red part of the light spectrum with color vision proven from 440 to 640 nm. Its eye is known to contain three visual pigments, rhodopsins, produced by an 11-cis-3-hydroxyretinal chromophore together with long wavelength (LWRh), blue (BRh) and UV (UVRh1) opsins. We now find that H. erato has a second UV opsin mRNA (UVRh2)—a previously undescribed duplication of this gene among Lepidoptera. To investigate its evolutionary origin, we screened eye cDNAs from 14 butterfly species in the subfamily Heliconiinae and found both copies only among Heliconius. Phylogeny-based tests of selection indicate positive selection of UVRh2 following duplication, and some of the positively selected sites correspond to vertebrate visual pigment spectral tuning residues. Epi-microspectrophotometry reveals two UV-absorbing rhodopsins in the H. erato eye with λmax = 355 nm and 398 nm. Along with the additional UV opsin, Heliconius have also evolved 3-hydroxy-DL-kynurenine (3-OHK)-based yellow wing pigments not found in close relatives. Visual models of how butterflies perceive wing color variation indicate this has resulted in an expansion of the number of distinguishable yellow colors on Heliconius wings. Functional diversification of the UV-sensitive visual pigments may help explain why the yellow wing pigments of Heliconius are so colorful in the UV range compared to the yellow pigments of close relatives lacking the UV opsin duplicate.
Laparoscopic Management of Tubal Ectopic Pregnancy

Objectives: To study the surgical morbidity associated with laparoscopic management of tubal ectopic pregnancy compared with that of open laparotomy. Methods: A retrospective study in an academic tertiary obstetrics and gynecology referral center was conducted from 2005 through 2007. Forty-nine patients who had pathology-confirmed tubal ectopic pregnancies were divided into 2 groups, laparoscopy (n=38) and laparotomy (n=11). The main outcome measures included operative time, blood loss, and complications. Results: No significant differences existed in gestational age, beta-hCG level, history of previous surgeries, pelvic inflammatory disease, or endometriosis. The laparotomy group included more patients with a history of previous ectopic pregnancy. The length of hospital stay following laparoscopic management was significantly less than that in the laparotomy group. Conclusion: Laparoscopic management of ectopic pregnancy can be the most beneficial procedure with maximum safety and efficacy.
Cross-Cultural Analysis: The Science and Art of Comparing the World's Modern Societies and Their Cultures

Contents Foreword Introduction PART 1: Understanding "Culture" The Concept of Culture Main characteristics of culture The elements of culture PART 2: Studying Culture Types of cross-cultural studies: merits and pitfalls Theoretical versus empirical perspectives Cross-cultural comparability Paper-and-pencil studies Data Analysis PART 3: Major Cross-Cultural Studies Cultural dimensions across modern nations PART 4: A Summary of the Observed Cultural Differences Across the Globe Appendix References
Evaluation of Mindful Sport Performance Enhancement (MSPE): A New Approach to Promote Flow in Athletes

The construct of mindfulness appears to be compatible with theories of flow and peak performance in sport. The present study assessed how Mindful Sport Performance Enhancement (MSPE), a new 4-week program, affected flow states, performance, and psychological characteristics of 11 archers and 21 golfers from the community. Participants completed trait measures of anxiety, perfectionism, thought disruption, confidence, mindfulness, and flow. They additionally provided data on their performances and state levels of mindfulness and flow. Analyses revealed that some significant changes in dimensions of the trait variables occurred during the training. Levels of state flow attained by the athletes also increased between the first and final sessions. The findings suggest that MSPE is a promising intervention to enhance flow, mindfulness, and aspects of sport confidence. An expanded workshop to allot more time for mindfulness practice is recommended for future studies.
Concise Review: Wharton's Jelly‐Derived Cells Are a Primitive Stromal Cell Population

Here, the literature was reviewed to evaluate whether a population of mesenchymal stromal cells derived from Wharton's jelly cells (WJCs) is a primitive stromal population. A clear case can be made for WJCs as a stromal population since they display the characteristics of MSCs as defined by the International Society for Cellular Therapy; for example, they grow as adherent cells with mesenchymal morphology, they are self‐renewing, they express cell surface markers displayed by MSCs, and they may be differentiated into bone, cartilage, adipose, muscle, and neural cells. Like other stromal cells, WJCs support the expansion of other stem cells, such as hematopoietic stem cells, are well‐tolerated by the immune system, and they have the ability to home to tumors. In contrast to bone marrow MSCs, WJCs have greater expansion capability, faster growth in vitro, and may synthesize different cytokines. WJCs are therapeutic in several different pre‐clinical animal models of human disease such as neurodegenerative disease, cancer, heart disease, etc. The preclinical work suggests that the WJCs are therapeutic via trophic rescue and immune modulation. In summary, WJCs meet the definition of MSCs. Since WJCs expand faster and to a greater extent than adult‐derived MSCs, these findings suggest that WJCs are a primitive stromal cell population with therapeutic potential. Further work is needed to determine whether WJCs engraft long‐term and display self‐renewal and multipotency in vivo and, as such, demonstrate whether Wharton's jelly cells are a true stem cell population.
Self-harm in relation to attachment theory and the cry of pain model: attachment insecurities and feelings of entrapment as vulnerability factors

Self-harm thoughts and behaviour have been found to be associated with a wide variety of distal and proximal factors; however, few studies have examined how these factors work together to increase the risk of self-harm. A key distal factor is a history of child and family adversity, which attachment theory views as a precursor of attachment insecurities that may increase the risk of later self-harm. A key proximal factor is the desire to escape from overwhelming distress, and Williams (2001) cry of pain model describes a process that could help better understand the reasons behind seeking escape via self-harm. This research investigated whether insecurely attached individuals tend to feel trapped and whether entrapment leads to self-harm thoughts (suicide ideation [Chapter 2] and thoughts about non-suicidal self-injury [Chapter 3]). This research also investigated whether feelings of entrapment among insecurely attached individuals varied as a function of problem-solving (as assessed with the Means-Ends Problem-Solving [MEPS] procedure [Chapter 4] and a diary study [Chapter 5]). The effect of stressful events on subsequent feelings of defeat and entrapment, and the role of attachment, was also examined using an experimental design (Chapter 6). Self-harm thoughts were common among insecurely attached individuals and among those who felt trapped. Entrapment was reported by insecurely attached individuals, but this feeling did not explain their self-harm thoughts nor did it vary as a function of problem-solving. In response to a laboratory stressor, attachment insecurities seem to exacerbate negative emotions. The findings suggest that assessment of attachment styles could help to identify individuals at risk of self-harm. Moreover, interventions aimed at reducing feelings of entrapment could decrease the risk of self-harm. Still, studies are needed to clarify the direction of the relationships between attachment, entrapment and self-harm, and the psychological mechanisms that might underlie these relationships.
Dopaminergic Neurons Exhibit an Age-Dependent Decline in Electrophysiological Parameters in the MitoPark Mouse Model of Parkinson's Disease

Dopaminergic neurons of the substantia nigra (SN) play a vital role in everyday tasks, such as reward-related behavior and voluntary movement, and excessive loss of these neurons is a primary hallmark of Parkinson's disease (PD). Mitochondrial dysfunction has long been implicated in PD and many animal models induce parkinsonian features by disrupting mitochondrial function. MitoPark mice are a recently developed genetic model of PD that lacks the gene for mitochondrial transcription factor A specifically in dopaminergic neurons. This model mimics many distinct characteristics of PD including progressive and selective loss of SN dopamine neurons, motor deficits that are improved by l-DOPA, and development of inclusion bodies. Here, we used brain slice electrophysiology to construct a timeline of functional decline in SN dopaminergic neurons from MitoPark mice. Dopaminergic neurons from MitoPark mice exhibited decreased cell capacitance and increased input resistance that became more severe with age. Pacemaker firing regularity was disrupted in MitoPark mice and ion channel conductances associated with firing were decreased. Additionally, dopaminergic neurons from MitoPark mice showed a progressive decrease of endogenous dopamine levels, decreased dopamine release, and smaller D2 dopamine receptor-mediated outward currents. Interestingly, expression of ion channel subunits associated with impulse activity (Cav1.2, Cav1.3, HCN1, Nav1.2, and NavB3) was upregulated in older MitoPark mice. The results describe alterations in intrinsic and synaptic properties of dopaminergic neurons in MitoPark mice occurring at ages both before and concurrent with motor impairment. These findings may help inform future investigations into treatment targets for prodromal PD. SIGNIFICANCE STATEMENT Parkinson's disease (PD) is the second most diagnosed neurodegenerative disorder, and the classic motor symptoms of the disease are attributed to selective loss of dopaminergic neurons of the substantia nigra. The MitoPark mouse is a genetic model of PD that mimics many of the key characteristics of the disease and enables the study of progressive neurodegeneration in parkinsonism. Here we have identified functional deficits in the ion channel physiology of dopaminergic neurons from MitoPark mice that both precede and are concurrent with the time course of behavioral symptomatology. Because PD is a progressive disease with a long asymptomatic phase, identification of early functional adaptations could lay the groundwork to test therapeutic interventions that halt or reverse disease progression.
An evaluation of traffic control devices and driver distraction on driver behavior at railway-highway grade crossings

The U.S. railroad system is made up of over 700 railroads operating on 140,000 miles of track, with more than 130,000 at-grade public railway-highway crossings, or intersections in which any part of a roadway intersects with railroad tracks. Safety at these railroad-highway grade crossings and the related traffic control devices used to communicate with drivers is of major concern. There are three factors that influence a driver’s behavior at a given crossing. First, traffic control devices, including warning devices at the railway-highway at-grade crossings, provide the driver with information. Second, assuming that the driver identifies the warning, the driver’s prior knowledge influences his or her expectancy regarding various railroad-highway grade crossing situations and, therefore, the way in which the driver responds to the hazard presented by the crossing. Finally, the driver’s own physiological (e.g., impaired) and psychological (e.g., distracted) state will modify the role that conspicuity and expectancy have on the driver’s behavior In order to address the gap that exists in our understanding of driver distraction at railway-highway at-grade crossings, two driving simulator experiments were conducted to determine the role that distraction has on the effectiveness of warning devices at grade crossings. In the first experiment, the role of distraction in reducing the benefit of typical crossbucks and flashing lights is evaluated. Participants were either engaged or not engaged in a distracting task, which included either a mock cell phone conversation or an in-vehicle task. Based upon the fact that distractions reduce the advantage of flashing lights and crossbucks, an evaluation of an alternative treatment that might be used to warn drivers of an approaching train was conducted. The question being addressed is whether these alternative markings, known as dynamic envelope pavement markings are an effective application for mitigating the effects of driver distraction at at-grade railway-highway grade crossings. Initial findings point to the fact that even when flashing lights and its respective signage and markings are properly working, a distracted driver is more likely to ignore the cues.
Genome-wide gene expression profiling in Arabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant

The phytohormone abscisic acid (ABA) plays important regulatory roles in many plant developmental processes including seed dormancy, germination, growth, and stomatal movements. These physiological responses to ABA are in large part brought about by changes in gene expression. To study genome-wide ABA-responsive gene expression we applied massively parallel signature sequencing (MPSS) to samples from Arabidopsis thaliana wildtype (WT) and abi1-1 mutant seedlings. We identified 1354 genes that are either up- or downregulated following ABA treatment of WT seedlings. Among these ABA-responsive genes, many encode signal transduction components. In addition, we identified novel ABA-responsive gene families including those encoding ribosomal proteins and proteins involved in regulated proteolysis. In the ABA-insensitive mutant abi1-1, ABA regulation of about 84.5% and 6.9% of the identified genes was impaired or strongly diminished, respectively; however, 8.6% of the genes remained appropriately regulated. Compared to other methods of gene expression analysis, the high sensitivity and specificity of MPSS allowed us to identify a large number of ABA-responsive genes in WT Arabidopsis thaliana. The database given in our supplementary material (http://jcs.biologists.org/supplemental) provides researchers with the opportunity to rapidly assess whether genes of interest may be regulated by ABA. Regulation of the majority of the genes by ABA was impaired in the ABA-insensitive mutant abi1-1. However, a subset of genes continued to be appropriately regulated by ABA, which suggests the presence of at least two ABA signaling pathways, only one of which is blocked in abi1-1.
Biased Beliefs, Asset Prices, and Investment: A Structural Approach

type="main"> We structurally estimate a model in which agents’ information processing biases can cause predictability in firms’ asset returns and investment inefficiencies. We generalize the neoclassical investment model by allowing for two biases—overconfidence and overextrapolation of trends—that distort agents’ expectations of firm productivity. Our model's predictions closely match empirical data on asset pricing and firm behavior. The estimated bias parameters are well identified and exhibit plausible magnitudes. Alternative models without either bias or with efficient investment fail to match observed return predictability and firm behavior. These results suggest that biases affect firm behavior, which in turn affects return anomalies.
Cation-independent Mannose 6-Phosphate Receptor

The 300-kDa cation-independent mannose 6-phosphate receptor (CI-MPR), which contains multiple mannose 6-phosphate (Man-6-P) binding sites that map to domains 3, 5, and 9 within its 15-domain extracytoplasmic region, functions as an efficient carrier of Man-6-P-containing lysosomal enzymes. To determine the types of phosphorylated N-glycans recognized by each of the three carbohydrate binding sites of the CI-MPR, a phosphorylated glycan microarray was probed with truncated forms of the CI-MPR. Surface plasmon resonance analyses using lysosomal enzymes with defined N-glycans were performed to evaluate whether multiple domains are needed to form a stable, high affinity carbohydrate binding pocket. Like domain 3, adjacent domains increase the affinity of domain 5 for phosphomannosyl residues, with domain 5 exhibiting ∼60-fold higher affinity for lysosomal enzymes containing the phosphodiester Man-P-GlcNAc when in the context of a construct encoding domains 5–9. In contrast, domain 9 does not require additional domains for high affinity binding. The three sites differ in their glycan specificity, with only domain 5 being capable of recognizing Man-P-GlcNAc. In addition, domain 9, unlike domains 1–3, interacts with Man8GlcNAc2 and Man9GlcNAc2 oligosaccharides containing a single phosphomonoester. Together, these data indicate that the assembly of three unique carbohydrate binding sites allows the CI-MPR to interact with the structurally diverse phosphorylated N-glycans it encounters on newly synthesized lysosomal enzymes.
Experimental and Analytical Studies on Lightning Surge Response of 500-kV Transmission Tower

This paper presents experimental and analytical studies on the lightning surge response of a 500-kV transmission tower without overhead lines. A number of experimental studies on the lightning surge response of transmission towers have been carried out. However, in these studies, the influence of the current injection angle was ignored. In this paper, an experimental investigation is conducted to assess the influences of the angle and direction of current injection. It is clearly shown that the lightning surge response of the tower depends on the angle and direction of current injection. These experimental results are compared with results calculated using numerical electromagnetic (EM)-field analysis. These calculated results agree with the experimental results. This suggests that numerical EM-field analysis can be employed for evaluating the surge response of a tower. Based on this, the surge response of a tower under a vertical lightning stroke is estimated. It is shown that the surge response under vertical current injection is approximately 30% higher than that under horizontal current injection. It is also shown that the angle and direction of the return stroke are important factors in determining the lightning surge response of a transmission tower.
Compound C Inhibits Vascular Smooth Muscle Cell Proliferation and Migration in an AMP-Activated Protein Kinase-Independent Fashion

6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrazolo[1,5-a] pyrimidine (compound C) is a cell-permeable pyrrazolopyrimidine derivative that acts as a potent inhibitor of AMP-activated protein kinase (AMPK). Although compound C is often used to determine the role of AMPK in various physiological processes, it also evokes AMPK-independent actions. In the present study, we investigated whether compound C influences vascular smooth muscle cell (SMC) function through the AMPK pathway. Treatment of rat aortic SMCs with compound C (0.02–10 μM) inhibited vascular SMC proliferation and migration in a concentration-dependent fashion. These actions of compound C were not mimicked or affected by silencing AMPKα expression or infecting SMCs with an adenovirus expressing a dominant-negative mutant of AMPK. In contrast, the pharmacological activator of AMPK 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside inhibited the proliferation and migration of SMCs in a manner that was strictly dependent on AMPK activity. Flow cytometry experiments revealed that compound C arrested SMCs in the G0/G1 phase of the cell cycle, and this was associated with a decrease in cyclin D1 and cyclin A protein expression and retinoblastoma protein phosphorylation and an increase in p21 protein expression. Finally, local perivascular delivery of compound C immediately after balloon injury of rat carotid arteries markedly attenuated neointima formation. These studies identify compound C as a novel AMPK-independent regulator of vascular SMC function that exerts inhibitory effects on SMC proliferation and migration and neointima formation after arterial injury. Compound C represents a potentially new therapeutic agent in treating and preventing occlusive vascular disease.
Not Trauma Alone: Therapy for Child Abuse Survivors in Family and Social Context

Forward. Preface. Part I: Abuse in Context: The Conceptual Framework. Abuse: The Trauma Model. Family: Beyond the Trauma Model. Alone: Growing Up in an Ineffective Family. Unprepared: The Legacy of an Ineffective Family Background. Impact: Intersecting Varieties of Abuse and Deficient Family Context. Society: Beyond Family Context. Part II: Treatment in Context: Foundations of the Therapeutic Model. Collaboration: Forming a Therapeutic Alliance. Conceptualization: Constructing Order from Chaos. Planning: Prioritizing Treatment Goals. Part III: Skills-Based Intervention: Implementation of the Therapeutic Model. Security: Managing and Modulating Distress. Focus: Fostering Experiential Presence and Continuity. Reasoning: Learning to Exercise Critical Thinking and Judgment. Coping: Breaking and Replacing Maladaptive Patterns. Liberation: Resolving the Trauma of Abuse. Transformation: The Miracle of Living Well. Part IV: Conclusion. Epilogue: The Inextricable Tie. References.