Miracle-dz/UniFAR-FTUs huggingface dataset:
Investigation of Global and Regional Myocardial Mechanics With 3-Dimensional Speckle Tracking Echocardiography and Relations to Hypertrophy and Fibrosis in Hypertrophic Cardiomyopathy

Background—In hypertrophic cardiomyopathy (HCM), heterogeneous myocardial hypertrophy and fibrosis are responsible for abnormalities of left ventricular (LV) function. We aimed to characterize LV global and regional myocardial mechanics in HCM, according to segmental hypertrophy and fibrosis. Methods and Results—Fifty-nine patients with HCM underwent standard echocardiography, 3-dimensional speckle tracking echocardiography, and cardiac magnetic resonance with late gadolinium enhancement (LGE); all 3 tests were <24 hours apart. Longitudinal, circumferential, and area strains were investigated according to the extent of LGE (no LGE, LGE<10%, and LGE≥10%), segmental thickness (≥15 versus <15 mm), and segmental LGE (LGE versus non-LGE). Attenuated global longitudinal strain showed association with extent of hypertrophy (indexed LV mass, r=0.32, P=0.01; maximum LV wall thickness, r=0.34, P=0.009; number of segments ≥15 mm, r=0.44, P<0.001), whereas enhanced global circumferential strain was correlated to LV global functional parameters (indexed end-systolic volume, r=0.47, P<0.001; ejection fraction, r=−0.75, P<0.001). Parameters of global myocardial mechanics showed no association with the extent of LGE; in contrast, the extent of LGE was associated with the extent of hypertrophy. All 3 deformation parameters were attenuated both in segments ≥15 mm in thickness and in those with LGE; adjusted analysis demonstrated that segmental presence of LGE was associated with additional attenuation in myocardial deformation. Conclusions—Both hypertrophy and fibrosis contribute to regional impairment of myocardial shortening in HCM. The extent of hypertrophy is the primary factor altering global myocardial mechanics. Circumferential myocardial shortening seems to be directly involved in preservation of LV systolic performance in HCM.
Marketing murderball: the influence of spectator motivation factors on sports consumption behaviours of wheelchair rugby spectators

This study examines the relationship between spectator motivation and sports consumption behaviours in the context of an adaptive sport. Respondents were spectators from five matches held in the Midwest United States involving registered United States Quad Rugby Association teams. The Motivation Scale for Sport Consumption (MSSC; Trail & James, 2001) was adapted to measure spectator motivation and predict repatronage intentions and online media consumption among wheelchair rugby spectators. Results indicated that two spectator motivation factors, physical skill and knowledge, were related to repatronage intentions. In addition, knowledge and vicarious achievement were found to be related to online media consumption.
Emergence and survival of Populus tremula seedlings under varying moisture conditions

Aspen produces large numbers of seeds, even though it mainly reproduces asexually with root suckers. The aim of this study was to find out how different moisture conditions affect emergence and survival of Populus tremula L. seedlings. This was studied with a sowing experiment (totally randomized factorial design). There were altogether 10 blocks, each containing 16 microsites and three treatments (sowing time, watering, sowing shelter) replicated twice in each block. Seedlings emerged on 56% of microsites. Sowing time affected seedling emergence. Both the proportion of microsites with seedlings and the number of seedlings per microsite were lower after first than after second sowing, when the weather was rainier. Watering increased the number of seedlings per microsite, but the proportion of micro sites with at least one seedling was not affected. Sowing shelter had a negative effect on the seedling emergence, especially after second sowing. The survival of seedlings was low (10%) and strongly dependent .
Age-related changes of protein SUMOylation balance in the AβPP Tg2576 mouse model of Alzheimer's disease

Alzheimer's disease (AD) is a complex disorder that affects the central nervous system causing a severe neurodegeneration. This pathology affects an increasing number of people worldwide due to the overall aging of the human population. In recent years SUMO protein modification has emerged as a possible cellular mechanism involved in AD. Some of the proteins engaged in the physiopathological process of AD, like BACE1, GSK3-β tau, AβPP, and JNK, are in fact subject to protein SUMO modifications or interactions. Here, we have investigated the SUMO/deSUMOylation balance and SUMO-related proteins during the onset and progression of the pathology in the Tg2576 mouse model of AD. We examined four age-stages (1.5, 3, 6, 17 months old) and observed shows an increase in SUMO-1 protein conjugation at 3 and 6 months in transgenic mice with respect to WT in both cortex and hippocampus. Interestingly this is paralleled by increased expression levels of Ubc9 and SENP1 in both brain regions. At 6 months of age also the SUMO-1 mRNA resulted augmented. SUMO-2-ylation was surprisingly decreased in old transgenic mice and was unaltered in the other time windows. The fact that alterations in SUMO/deSUMOylation equilibrium occur from the early phases of AD suggests that global posttranslational modifications may play an important role in the mechanisms underlying disease pathogenesis, thus providing potential targets for pharmacological interventions.
On our feet: feasibility trial of an intervention to reduce sedentary behavior and increase physical activity

Sedentary behavior (SB) is emerging as an independent risk factor for cardiovascular disease and type 2 diabetes (Katzmarzyk, 2010, Owen et al., 2010), and is a target for intervention. This study examined effects of a Social Cognitive intervention to increase self-efficacy (SE) for reducing SB and increasing PA. Female members (Mage=58.5, SD=12.5 yrs) from seven weight loss support clubs were enrolled in a 6-week intervention (n=40) or waitlisted (n=24) based on club randomization. The intervention, delivered via group sessions and email, used mastery feedback from goal-setting activities along with behavioral cues and modeling to reduce SB and increase steps. Quantitative and qualitative process evaluation data were collected throughout. PA and SB were measured by accelerometers. SE (to reduce SB, to increase light & moderate PA) were measured pre, mid, and post. Repeated-measures MANOVA found no significant change over time or Group x Time interaction for behavior. A significant effect for time was noted for SE to reduce SB (F=3.34, p<.05) and the Group x Time interaction approached significance. SE decreased at mid-point, but increased for the intervention group while the waitlist group continued to fall. Differences between rural and urban women in SB (F=4.69, p<0.04) and SE to reduce SB (F=4.75, p<0.05), were significant, with rural participants having less SB and lower SE to reduce SB than urban peers. Participants' perceptions were examined to provide a contextualized understanding of SB and methods to change behavior. Analysis identified compliance barriers, including required sitting, accuracy of self-monitoring, work and family responsibilities and questions of relevance. Significant differences in SE to reduce SB were seen when compliant participants were compared to non-compliant participants (F=2.44, p<0.05). Behavioral cues and modeled behaviors were less impactful than anticipated. Participants were receptive to monitoring PA via pedometer, though challenges in self-monitoring SB were noted. The role of SE in changing SB needs further study. Findings suggest that interventions must consider contextual factors, such as location and occupation as well as individual factors (SE) and barriers. Future studies should consider the effect of modifying the built-environment on SE to reduce SB and improve methods for self-monitoring.
Assisted conception and the risk of CHD: a case–control study

Abstract Epidemiological studies suggest a higher prevalence of congenital malformations in children conceived through assisted reproductive technologies. There are a few studies that address CHD specifically and most have examined data from registries. We examined the relationship between CHD and assisted conception using data collected in a specialist paediatric cardiac service in the United Kingdom. Between April, 2010 and July, 2011, the parents of children attending paediatric cardiology clinics at the Royal Brompton Hospital, London, were invited to complete a questionnaire that enquired about the nature of their child’s conception, the route for their original referral, and a number of potential confounding exposures. “Cases” were defined as children diagnosed with one or more carefully defined CHDs and “controls” as those with normal hearts. Of 894 new attendees with complete data, half of them were cases (n=410, 45.9%). The overall prevalence of assisted conception was 5.4% (n=44). Logistic regression analysis demonstrated a non-significant increase in the crude odds for the use of assisted reproduction (odds ratio 1.21, 95% confidence interval 0.66–2.22) in this group. After adjustment for gestation, parity, year of birth, and maternal age, the odds ratio reduced (odds ratio 0.95, 95% confidence interval 0.48–1.88). Increased rates of assisted conception were observed in a number of CHD subgroups, although no significant differences were found. These findings do not suggest an overall association between CHD and assisted reproduction in this population.
Chemical characteristics and pasting properties of commercial Slovak common and spelt wheat flours.

Th e objective of this paper was to show diff erences among qualitative parameters of commercially produced Slovak fi ne and wholemeal wheat fl ours prepared from Triticum aestivum L. (common wheat) and Triticum aestivum ssp. spelta L. (spelt wheat). Th e selected chemical parameters (protein, starch, ash, glucose, fructose, sucrose, maltose, pH, lactic acid, acetic acid, citric acid, succinic acid, calcium, iron and zinc), gluten characteristics (wet and dry gluten, extensibility of gluten and swelling of gluten) and pasting properties (pasting temperature, peak viscosity, hot paste viscosity, peak viscosity temperature and breakdown value) of fl ours were analysed. Spelt wheat wholemeal fl our produced in ecological system of farming diff ered from other commercially produced wheat fl ours mainly in ash (1.82 %), proteins (16.5 %), wet gluten (43.2 % in dry matter), minerals (Ca, Fe, Zn) and organic acids content. Maltose was found as the most predominate saccharide in the common wheat fi ne fl our (0.46 g/100g) and sucrose was prevalent in common and spelt wheat wholemeal fl ours (0.68 and 0.76 g/100g). It was also stated that commercially produced common wheat fi ne fl our was characterised by lower pasting temperature (59 °C), peak viscosity (575 BU) and breakdown value (210 BU) than common and spelt wheat wholemeal fl ours.
The impact of electronic word of mouth on a tourism destination choice: Testing the theory of planned behavior (TBP)

Purpose – In an environment in which there has been a reduction in consumer trust of both organizations and advertising, as well as a decrease in television advertising, word of mouth (WOM) offers a way to obtain a significant competitive advantage. WOM is especially important in the hospitality and tourism industry, whose intangible products are difficult to evaluate prior to their consumption. When WOM becomes digital, the large‐scale, anonymous, ephemeral nature of the Internet induces new ways of capturing, analyzing, interpreting, and managing the influence that one consumer may have on another. The purpose of this paper is to investigate the impact of electronic WOM (eWOM) on a tourism destination choice, using the theory of planned behaviour (TPB). Further, an effort was employed to determine the effect of past travel experience on using eWOM and TPB constructs.Design/methodology/approach – A structural equation modeling procedure was applied to the examination of the influences of eWOM on attitude.
Center for Policy Research Working Paper No . 130 MEDICAL TECHNOLOGY AND THE PRODUCTION OF HEALTH CARE

This paper investigates the factors that determine differences across OECD countries in health outcomes, using data on life expectancy at age 65, over the period 1960 to 2007. We estimate a production function where life expectancy depends on health and social spending, lifestyle variables, and medical innovation. Our first set of regressions includes a set of observed medical technologies by country. Our second set of regressions proxy technology using a spatial process. The paper also tests whether in the long-run countries tend to achieve similar levels of health outcomes. Our results show that health spending has a significant and mild effect on health outcomes, even after controlling for medical innovation. However, its short-run adjustments do not seem to have an impact on health care productivity. Spatial spill overs in life expectancy are significant and point to the existence of interdependence across countries in technology adoption. Furthermore, nations with initial low levels of life expectancy tend to catch up with those with longer-lived populations. JEL No. C31, C33, H51
'(Naughty) Departures': Expertise, Orthodoxy and the Role of Theory in the Practice of Mediation

This paper discusses the nature of mediator expertise, and whether skilled practitioners’ implicit knowledge can be elicited and made available to others. Regardless of setting, a considerable body of research suggests that experts do not necessarily behave according to the conventions of their field, or 'by the book'. Although expertise is generally acknowledged as domain–specific, some authorities trace sufficient similarities across disciplines in order to propose a general theory of expertise and expert performance. This general theory may be extrapolated to provide valuable insights into understanding and supporting the practice of experienced mediators, as well as training new practitioners. Also touched upon in this paper are mediation orthodoxy and ideologies, the findings of research programmes involving mediators considered by others (i.e. case referrers) to be experts, and the notorious theory/practice divide. The paper concludes with a short discussion of the benefits and disadvantages of expertise models.
Pausing during Reverse Transcription Increases the Rate of Retroviral Recombination

ABSTRACT Retroviruses package two copies of genomic RNA into viral particles. During the minus-sense DNA synthesis step of reverse transcription, the nascent DNA can transfer multiple times between the two copies of the genome, resulting in recombination. The mechanism for this process is similar to the process of obligate strand transfers mediated by the repeat and primer binding site sequences. The location at which the DNA 3′ terminus completely transfers to the second RNA strand defines the point of crossover. Previous work in vitro demonstrated that reverse transcriptase pausing has a significant impact on the location of the crossover, with a proportion of complete transfer events occurring very close to pause sites. The role of pausing in vivo, however, is not clearly understood. By employing a murine leukemia virus-based single-cycle infection assay, strong pausing was shown to increase the probability of recombination, as reflected in the reconstitution of green fluorescent protein expression. The infection assay results were directly correlated with the presence of strong pause sites in reverse transcriptase primer extension assays in vitro. Conversely, when pausing was diminished in vitro, without changing the sequence of the RNA template involved in recombination, there was a significant reduction in recombination in vivo. Together, these data demonstrate that reverse transcriptase pausing, as observed in vitro, directly correlates with recombination during minus-sense DNA synthesis in vivo.
Determinant roles of environmental contamination and noncompliance with standard precautions in the risk of hepatitis C virus transmission in a hemodialysis unit.

BACKGROUND Nosocomial transmission is the second most frequent cause of hepatitis C virus (HCV) infection. A prospective observational study was conducted to assess the roles of environmental contamination and noncompliance with standard precautions in HCV cross-transmission in a hemodialysis unit. METHODS Patients undergoing chronic hemodialysis in a French university hospital unit were systematically screened, revealing 2 sporadic cases of HCV transmission. An investigation was launched to determine whether the patients were infected in the hemodialysis unit and the possible roles of environmental contamination and noncompliance with standard precautions. We examined possible relationships among new cases of HCV infection, environmental contamination by blood and HCV RNA, and compliance with guidelines on hand hygiene and glove use. RESULTS Two patients experienced seroconversion to HCV during the study period. Phylogenetic analyses showed that 1 of these patients was infected with the same strain as that affecting a chronically infected patient also treated in the unit. Of 740 environmental surface samples, 82 (11%) contained hemoglobin; 6 (7%) of those contained HCV RNA. The rate of compliance with hand hygiene was 37% (95% confidence interval, 35%-39%), and gloves were immediately removed after patient care in 33% (95% confidence interval, 29%-37%) of cases. A low ratio of nurses to patients and poor hand hygiene were independent predictors of the presence of hemoglobin on environmental surfaces. CONCLUSION Blood-contaminated surfaces may be a source of HCV cross-transmission in a hemodialysis unit. Strict compliance with hand hygiene and glove use and strict organization of care procedures are needed to reduce the risk of HCV cross-transmission among patients undergoing hemodialysis.
Oral glucose solution to alleviate pain induced by intramuscular injections in preterm infants.

PURPOSE The purpose was to assess the effectiveness of 5% oral glucose solution in reducing pain in preterm infants during intramuscular injection. DESIGN AND METHODS We conducted a prospective, randomized, controlled, unblinded trial to investigate the effect of glucose solution on the pain of intramuscular injection in preterm infants (N = 80). RESULTS Infants who received oral glucose had significantly lower pain scores (p < .001), less crying time (p < .001), higher oxygen saturation (p < .001), and lower heart rate after compared with during the procedure (p = .02). PRACTICE IMPLICATIONS Our results suggest that oral glucose, even if used in the lowest dose, may have a pain-relieving effect in preterm infants if administered pre-procedure.
ZHOUPI controls embryonic cuticle formation via a signalling pathway involving the subtilisin protease ABNORMAL LEAF-SHAPE1 and the receptor kinases GASSHO1 and GASSHO2

Seed production in angiosperms requires tight coordination of the development of the embryo and the endosperm. The endosperm-specific transcription factor ZHOUPI has previously been shown to play a key role in this process, by regulating both endosperm breakdown and the formation of the embryonic cuticle. To what extent these processes are functionally linked is, however, unclear. In order to address this issue we have concentrated on the subtilisin-like serine protease encoding gene ABNORMAL LEAF-SHAPE1. Expression of ABNORMAL LEAF-SHAPE1 is endosperm specific, and dramatically decreased in zhoupi mutants. We show that, although ABNORMAL LEAF-SHAPE1 is required for normal embryonic cuticle formation, it plays no role in regulating endosperm breakdown. Furthermore, we show that re-introducing ABNORMAL LEAF-SHAPE1 expression in the endosperm of zhoupi mutants partially rescues embryonic cuticle formation without rescuing their persistent endosperm phenotype. Thus, we conclude that ALE1 can normalize cuticle formation in the absence of endosperm breakdown, and that ZHOUPI thus controls two genetically separable developmental processes. Finally, our genetic study shows that ZHOUPI and ABNORMAL LEAF-SHAPE1 promotes formation of embryonic cuticle via a pathway involving embryonically expressed receptor kinases GASSHO1 and GASSHO2. We therefore provide a molecular framework of inter-tissue communication for embryo-specific cuticle formation during embryogenesis.
Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing

The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTL) shows promise in the treatment of cancer and infectious diseases. We utilize adeno-associated virus-(AAV-) based antigen gene-loaded dendritic cells (DCs) to stimulate such antigen-specific CTL. Yet further improvements in CTL stimulation and killing may result by gene delivery of various Th1-response interferons/cytokines, such as interferon γ (IFN-γ), as the delivered gene can continuously produce that interferon. However which immune cell type should optimally express IFN-γ is unclear as the phenotypes of both DC and T cells are enhanced by it. Here, we used AAV to compare and contrast IFN-γ gene delivery into DC or T cells, and versus the addition of exogenous IFN-γ, for stimulating carcinoembryonic antigen-(CEA-) specific CTL. It was found that AAV/IFN-γ delivery into T cells (autocrine) resulted in T cell populations with the highest CD8(+)/CD4(+) ratio, highest IFN-γ(+)/IL-4(+) ratio, highest CD69(+),CD8(+) levels, and lowest CD4(+)/CD25(+) levels, all consistent with the strongest Th1 response. Most importantly, AAV/IFN-γ transduction of T cells resulted in antigen-specific T cell populations with the highest killing capabilities, 49% above other treatments. These data strongly suggest that AAV/IFN-γ autocrine gene delivery into T cells is worthy of further study towards maximizing the generation of antigen-specific anticancer CTL killers.
Robotic etiquette: Results from user studies involving a fetch and carry task

This paper presents results, outcomes and conclusions from a series of Human Robot Interaction (HRI) trials which investigated how a robot should approach a human in a fetch and carry task. Two pilot trials were carried out, aiding the development of a main HRI trial with four different approach contexts under controlled experimental conditions. The findings from the pilot trials were confirmed and expanded upon. Most subjects disliked a frontal approach when seated. In general, seated humans do not like to be approached by a robot directly from the front even when seated behind a table. A frontal approach is more acceptable when a human is standing in an open area. Most subjects preferred to be approached from either the left or right side, with a small overall preference for a right approach by the robot. However, this is not a strong preference and it may be disregarded if it is more physically convenient to approach from a left front direction. Handedness and occupation were not related to these preferences. Subjects do not usually like the robot to move or approach from directly behind them, preferring the robot to be in view even if this means the robot taking a physically non-optimum path. The subjects for the main HRI trials had no previous experience of interacting with robots. Future research aims are outlined and include the necessity of carrying out longitudinal trials to see if these findings hold over a longer period of exposure to robots.