Miracle-dz/UniFAR-FTUs huggingface dataset:
Studies with Lysine N6-Hydroxylase. Effect of a Mutation in the Assumed FAD Binding Site on Coenzyme Affinities and on Lysine Hydroxylating Activity

Abstract The proposed FAD binding site of L-lysine N6-hydroxylase (EC 1.14.13.99) exhibits an unusual proline in a position where a highly conserved glycine is found in other FAD dependent hydroxylases. We have studied the role of this proline by mutating it to glycine in [P14G]aerA, which was expressed in Escherichia coli M15-2 and purified to homogeneity. The mutation has marked effects on the affinities of the cofactors FAD and NADPH as well as the substrate, lysine. Compared to the wild-type enzyme, the activity vs. pH profile of the mutant protein indicates a shift of the apparent pK'as (7.8 and 8.7 for wild-type and 6.8 and 7.7 for the P14G-mutant enzyme) and of the activity maximum (pH 8 for wild-type and pH 7 for the P14G-mutant enzyme). While the activity of the mutant enzyme is much lower under conditions found to be optimal for the wild-type enzyme, adjustment of substrate and cofactor concentrations and pH leads to comparable activities for the mutant enzyme. These results suggest that the proline fulfils an important structural role in the proposed FAD binding site.
Physical aging of a polyetherimide: Creep and DSC measurements

Creep and differential scanning calorimetry (DSC) measurements have been used to study the physical aging behavior of a polyetherimide. Isothermal aging temperatures ranged from 160°C to T g with aging times ranging from 10 min to 8 days. The only measurable effect of physical aging on the short-time creep curves is a shift of the creep compliance to longer times. Andrade plots of the compliance versus the cube root of time are linear at short times with the slope β decreasing with increasing aging time to a constant value once equilibrium is reached. Log β 3 is related directly to the degree to which the creep curves shift to longer times with physical aging, and is used in this work as a measure of physical aging. A reduced curve of log β 3 versus log aging time is obtained for the aging temperatures investigated by appropriate vertical and horizontal shifts. The enthalpy change during aging increases linearly with the logarithm of the aging time, t a , leveling off at equilibrium at values which increase with decreasing aging temperature. Hence, both nonequilibrium and equilibrium temperature shift factors can be calculated from the DSC data. Good agreement is observed between the equilibrium temperature shift factors obtained from the creep and DSC data. The temperature dependence of the nonequilibrium temperature shift factors is found to be an order of magnitude smaller than that of the equilibrium shift factors. The time scales to reach equilibrium for enthalpy and for mechanical measurements are found to be the same within experimental error.
Cesarean Section Is Associated with Increased Peripheral and Central Adiposity in Young Adulthood: Cohort Study

Background Cesarean section (CS) has been associated with obesity, measured by body mass index (BMI), in some studies. It has been hypothesized that this association, if causal, might be explained by changes in gut microbiota. However, little is known about whether CS is also associated with increased adiposity as measured by indicators other than BMI. Objective: To assess the association between CS and indicators of peripheral and central adiposity in young adults. Methods The study was conducted on 2,063 young adults aged 23 to 25 years from the 1978/79Ribeirão Preto birth cohort, São Paulo, Brazil. CS was the independent variable. The anthropometric indicators of adiposity were: waist circumference (WC), waist-height ratio (WHtR), waist-hip ratio (WHR), tricipital skinfold (TSF), and subscapular skinfold (SSF). The association between CS and indicators of adiposity was investigated using a Poisson model, with robust adjustment of variance and calculation of incidence rate ratio (IRR) with 95% confidence interval (95%CI), and adjustment for birth variables. Results Follow-up rate was 31.8%. The CS rate was 32%. Prevalences of increased WC, WHtR, WHR were 32.1%, 33.0% and 15.2%, respectively. After adjustment for birth variables, CS was associated with increased risk of adiposity when compared to vaginal delivery: 1.22 (95%CI 1.07; 1.39) for WC, 1.25 (95%CI 1.10;1.42) for WHtR, 1.45 (95%CI 1.18;1.79) for WHR, 1.36 (95%CI 1.04;1.78) for TSF, and 1.43 (95%CI 1.08;1.91) for SSF. Conclusion Subjects born by CS had a higher risk for increased peripheral and central adiposity during young adult age compared to those born by vaginal delivery. The association of CS with adiposity was consistently observed for all indicators and was robust after adjustment for a variety of early life confounders.
Bar Evolution over the Last 8 Billion Years: A Constant Fraction of Strong Bars in the GEMS Survey

One-third of present-day spirals host optically visible strong bars that drive their dynamical evolution. However, the fundamental question of how bars evolve over cosmological times has yet to be resolved, and even the frequency of bars at intermediate redshifts remains controversial. We investigate the frequency of bars out to z ~ 1 drawing on a sample of 1590 galaxies from the Galaxy Evolution from Morphologies and SEDs survey, which provides morphologies from Hubble Space Telescope Advanced Camera for Surveys (ACS) two-band images and accurate redshifts from the COMBO-17 survey. We identify spiral galaxies using three independent techniques based on the Sersic index, concentration parameter, and rest-frame color. We characterize bar and disk features by fitting ellipses to F606W and F850LP images, using the two bands to minimize shifts in the rest-frame bandpass. We exclude highly inclined (i > 60°) galaxies to ensure reliable morphological classifications and apply different completeness cuts of MV ≤ -19.3 and -20.6. More than 40% of the bars that we detect have semimajor axes a < 0. ″5 and would be easily missed in earlier surveys without the small point-spread function of ACS. The bars that we can reliably detect are fairly strong (with ellipticities e ≥ 0.4) and have a in the range ~1.2-13 kpc. We find that the optical fraction of such strong bars remains at ~30% ± 6% from the present day out to look-back times of 2-6 Gyr (z ~ 0.2-0.7) and 6-8 Gyr (z ~ 0.7-1.0); it certainly shows no sign of a drastic decline at z > 0.7. Our findings of a large and similar bar fraction at these three epochs favor scenarios in which cold gravitationally unstable disks are already in place by z ~ 1 and where on average bars have a long lifetime (well in excess of 2 Gyr). The distributions of structural bar properties in the two slices are, however, not statistically identical and therefore allow for the possibility that the bar strengths and sizes may evolve over time.
Maximum boost control of the Z-source inverter

This paper explores control methods for the Z-source inverter and their relationships of voltage boost versus modulation index. A maximum boost control is presented to produce the maximum voltage boost (or voltage gain) under a given modulation index. The control method, relationships of voltage gain versus modulation index, and voltage stress versus voltage gain are analyzed in detail and verified by simulation and experiment.
Peroxynitrite Inactivation of Tyrosine Hydroxylase: Mediation by Sulfhydryl Oxidation, not Tyrosine Nitration

Tyrosine hydroxylase (TH) is the initial and rate-limiting enzyme in the biosynthesis of dopamine (DA). TH activity is significantly diminished in Parkinson's disease (PD) and by the neurotoxic amphetamines, thereby accentuating the reductions in DA associated with these conditions. Reactive oxygen and nitrogen species have been implicated in the damage to DA neurons seen in PD and in reaction to amphetamine drugs of abuse, so we investigated the hypothesis that peroxynitrite (ONOO−) could interfere with TH catalytic function. ONOO− caused a concentration-dependent inactivation of TH. The inactivation was associated with tyrosine nitration (maximum of four tyrosine residues nitrated per TH monomer) and extensive sulfhydryl oxidation. Tetranitromethane, which causes sulfhydryl oxidation at pH 6 and 8 but which nitrates tyrosines only at pH 8, inactivated TH equally at either pH. Bicarbonate protected TH from ONOO−-induced inactivation and sulfhydryl oxidation but increased significantly tyrosine nitration. PNU-101033 blocked ONOO−-induced tyrosine nitration in TH but could not prevent enzyme inactivation or sulfhydryl oxidation. Together, these results indicate that the inactivation of TH by ONOO− is mediated by sulfhydryl oxidation. The coincident nitration of tyrosine residues appears to exert little influence over TH catalytic function.
The role of practice in fact retrieval.

Four experiments examined the relationship among massive practice, speed-up of memory retrieval, and the reduction of long-term memory interference. All experiments employed a multiday fact recognition procedure. Interference was varied by a fan manipulation: Concepts could appear in more than one fact (fan) or only one fact (no fan). We found that fact retrieval speeds up as a power function of days of practice but that the number of daily repetitions beyond four produced little or no impact on reaction time. In addition, interference decreased in proportion to the degree of practice but did not disappear even with 25 days of practice. Practice on specific facts and practice on the general task had multiplicative effects in reducing recognition time. Further, general task practice was found to decrease interference, suggesting that general central processes were speeding up. Most but not all of these practice effects and their interactions with interference are predicted by the ACT* model of fact retrieval.
Cultural differences in the dialectical and non-dialectical emotional styles and their implications for health

Previous cross-cultural studies have repeatedly demonstrated that East Asians are more likely to show a dialectical emotional style than Americans, but do not distinguish between specific types of dialectical emotional styles. Using an age diverse sample, we found that compared to Americans, Japanese are more likely to experience both positive and negative emotions moderately frequently (i.e., moderate dialectical), but are no more likely to experience them frequently (i.e., high dialectical). Thus, dialectical emotions prevalent in East Asia may be characterised by a “middle way” rather than by emotional extremes. Furthermore, we explored whether dialectical emotion types are associated with better health profiles depending on cultural background. Our results show that the moderate dialectical type is associated with fewer physical symptoms in Japan than in the USA. Together, these findings show the cultural differences in the experience of balanced positive and negative emotions and their health correlates.
Intermittent Hypoxia-Induced Carotid Body Chemosensory Potentiation and Hypertension Are Critically Dependent on Peroxynitrite Formation

Oxidative stress is involved in the development of carotid body (CB) chemosensory potentiation and systemic hypertension induced by chronic intermittent hypoxia (CIH), the main feature of obstructive sleep apnea. We tested whether peroxynitrite (ONOO−), a highly reactive nitrogen species, is involved in the enhanced CB oxygen chemosensitivity and the hypertension during CIH. Accordingly, we studied effects of Ebselen, an ONOO− scavenger, on 3-nitrotyrosine immunoreactivity (3-NT-ir) in the CB, the CB chemosensory discharge, and arterial blood pressure (BP) in rats exposed to CIH. Male Sprague-Dawley rats were exposed to CIH (5% O2, 12 times/h, 8 h/day) for 7 days. Ebselen (10 mg/kg/day) was administrated using osmotic minipumps and BP measured with radiotelemetry. Compared to the sham animals, CIH-treated rats showed increased 3-NT-ir within the CB, enhanced CB chemosensory responses to hypoxia, increased BP response to acute hypoxia, and hypertension. Rats treated with Ebselen and exposed to CIH displayed a significant reduction in 3-NT-ir levels (60.8 ± 14.9 versus 22.9 ± 4.2 a.u.), reduced CB chemosensory response to 5% O2 (266.5 ± 13.4 versus 168.6 ± 16.8 Hz), and decreased mean BP (116.9 ± 13.2 versus 82.1 ± 5.1 mmHg). Our results suggest that CIH-induced CB chemosensory potentiation and hypertension are critically dependent on ONOO− formation.
Abdominal Obesity Is Characterized by Higher Pulse Pressure: Possible Role of Free Triiodothyronine

Objective. This study examined whether obesity is characterized by higher 24 h mean pulse pressure (24 h mean SBP-24 h mean DBP) and whether free thyroid hormones (FT3 and FT4) have a relationship with 24 h mean pulse pressure. Methods. A total of 231 euthyroid overweight and obese patients, 103 women and 128 men, aged 18–68 yrs, normotensive (n = 69) or with recently developed hypertension (n = 162), never treated with antihypertensive drugs, were investigated. Fasting insulin, TSH, FT3, FT4, glucose, and lipid serum concentrations were measured. Waist circumference was measured as an indirect parameter of central fat accumulation. Ambulatory blood pressure monitoring (ABPM) was performed. Results. 24 h mean pulse pressure (PP) showed a significant positive correlation with BMI (P < 0.001), waist circumference (P < 0.001), and FT3 (P < 0.001) and insulin serum levels (P < 0.05). When a multivariate analysis was performed, and 24 h PP was considered as the dependent variable, and waist circumference, FT3, insulin, male sex, and age as independent parameters, 24 h mean PP maintained a significant association only with waist circumference (P < 0.001) and FT3 levels (P < 0.05). Conclusion. Our results suggest that FT3 per se may contribute to higher pulse pressure in obese subjects.
A pulsation analysis of K2 observations of the subdwarf B star PG 1142-037 during Campaign 1: A subsynchronously rotating ellipsoidal variable

We report a new subdwarf B (sdB) pulsator, PG1142-037, discovered during the first full-length campaign of K2, the two-gyro mission of the Kepler space telescope. Fourteen periodicities have been detected between 0.9 and 2.5 hours with amplitudes below 0.35 ppt. We have been able to associate all of the pulsations with low-degree, ell<=2 modes. Follow-up spectroscopy of PG1142 has revealed it to be in a binary with a period of 0.54 days. Phase-folding the K2 photometry reveals a two-component variation including both Doppler boosting and ellipsoidal deformation. Perhaps the most surprising and interesting result is the detection of an ellipsoidal, tidally distorted variable with no indication of rotationally-induced pulsation multiplets. This indicates that the rotation period is longer than 45 days, even though the binary period is near 13 hours.
Occupational human immunodeficiency virus infection in health care workers: worldwide cases through September 1997.

The average estimated risk of human immunodeficiency virus (HIV) infection for health care workers following a percutaneous or mucous exposure is <0.5% in incidence studies, although a case-control study suggests it is much higher for highest-risk percutaneous exposure. To characterize exposures resulting in HIV transmission, we reviewed available data on occupational cases reported worldwide, identifying 94 documented and 170 possible cases. The majority of documented infections occurred in nurses, after contact with the blood of a patient with AIDS by means of percutaneous exposure, with a device placed in an artery or vein. High-exposure job categories, e.g., midwives and surgeons, are represented mostly among possible cases. Transmission occurred also through splashes, cuts, and skin contaminations, and in some cases despite postexposure prophylaxis with zidovudine. Health care workers could benefit if these data were incorporated in educational programs designed to prevent occupational bloodborne infections.
Tensile properties and morphology of the dynamically cured EPDM and PP/HDPE ternary blends

The tensile properties and morphology of the polyolefin ternary blends of ethylenepropylene–diene terpolymer (EPDM), polypropylene and high density polyethylene were studied. Blends were prepared in a laboratory internal mixer where EPDM was cured in the presence of PP and HDPE under shear with dicumyl peroxide (DCP). For comparison, blends were also prepared from EPDM which was dynamically cured alone and blended with PP and HDPE later (cure–blend). The effect of DCP concentration, intensity of the shear mixing, and rubber/plastics composition was studied. The tensile strength and modulus increased with increasing DCP concentration in the blends of EPDM-rich compositions but decreased with increasing DCP concentration in blends of PP-rich compositions. In the morphological analysis by scanning electron microscopy (SEM), the small amount of EPDM acted as a compatibilizer to HDPE and PP. It was also revealed that the dynamic curing process could reduce the domain size of the crosslinked EPDM phase. When the EPDM forms the matrix, the phase separation effect becomes dominant between the EPDM matrix and PP or HDPE domain due to the crosslinking in the matrix.
PlanHab (Planetary Habitat Simulation): the combined and separate effects of 21 days bed rest and hypoxic confinement on human skeletal muscle miRNA expression

The study concerns effects of 21 days of sustained bedrest and hypoxia, alone and in combination, on skeletal muscle microRNA (miRNA) expression. It is expected that astronauts undertaking long‐duration missions will be exposed not only to microgravity but also to a hypoxic environment. The molecular machinery underlying microgravity‐induced alterations in skeletal muscle structure and function is still largely unknown. One possible regulatory mechanism is altered expression of miRNAs, a group of noncoding RNAs which down‐regulate many different target genes through increased degradation or translation of their messenger RNA. Thirteen healthy men underwent three 21‐day interventions, interspersed by 4‐month washout periods: horizontal bedrest in normoxia, bedrest in hypoxia, ambulation in hypoxia. The level of hypoxia corresponded to 4000 m altitude. miRNAs from v. lateralis muscle biopsies were analyzed using a microarray covering ≈4000 human miRNAs. Sixteen mature miRNAs were up‐regulated and three down‐regulated after bedrest. The magnitudes of these changes were small and a large portion of the miRNAs affected by bedrest was also differentially expressed after washout periods. In fact, the number of differentially expressed probe sets over time was substantially larger than what could be detected after bedrest. Still, the majority of the miRNAs (let‐7, miR‐15, miR‐25, miR‐199, miR‐133) that were differentially expressed following bedrest, belong to miRNA families previously reported in the context of muscle physiology, in particular to respond to changes in mechanical loading. Since only minor changes in miRNA expression could be detected after bedrest, our data indicate miRNA to play only a minor role in the substantial change in muscle phenotype seen with unloading.
Organ manifestations and long-term outcome of Fabry disease in patients with the GLA haplotype D313Y

Objectives The severity of Fabry disease is dependent on the type of mutation in the α-galactosidase A (AgalA) encoding gene (GLA). This study focused on the impact of the GLA haplotype D313Y on long-term organ involvement and function. Setting and participants In this monocentric study, all participants presenting with the D313Y haplotype between 2001 and 2015 were comprehensively clinically investigated at baseline and during a 4-year follow-up if available. Five females and one male were included. Primary and secondary outcome measures Cardiac, nephrological, neurological, laboratory and quality of life data. Results AgalA enzyme activity in leucocytes (0.3±0.9 nmol/min/mg protein (mean±SD)) and serum lyso-Gb3 (0.6±0.3 ng/mL at baseline) were in normal range in all patients. Cardiac morphology and function were normal (left-ventricular (LV) ejection fraction 66±8%; interventricular septum 7.7±1.4 mm; LV posterior wall 7.5±1.4 mm; normalised LV mass in MRI 52±9 g/m2; LV global longitudinal strain −21.6±1.9%) and there were no signs of myocardial fibrosis in cardiac MRI. Cardiospecific biomarkers were also in normal range. Renal function was not impaired (estimated glomerular filtration rate MDRD 103±15 mL/min; serum-creatinine 0.75±0.07 mg/dL; cystatin-c 0.71±0.12 mg/L). One female patient (also carrying a Factor V Leiden mutation) had a transitory ischaemic attack. One patient showed white matter lesions in brain MRI, but none had Fabry-associated pain attacks, pain crises, evoked pain or permanent pain. Health-related quality of life analysis revealed a reduction in individual well-being. At long-term follow-up after 4 years, no significant change was seen in any parameter. Conclusions The results of the current study suggest that the D313Y genotype does not lead to severe organ manifestations as seen in genotypes known to be causal for classical FD.
Forced air plasma treatment for enhanced adhesion of polypropylene and polyethylene

This paper describes our investigation of the effects of forced air plasma treatment on polypropylene and polyethylene. The morphology of the treated surfaces has been carefully examined using a variety of tools including optical profiling. The complex surface morphology was observed to change with increasing treatment and varying intensity of the treatment over the surface. Optimum treatment conditions have been deduced using surface energy determinations and can be compared with the morphological changes. Determinations of surface energy, both the polar and non-polar components, have been made after exposure to varying moisture conditions for varying times. Different results are obtained for different environments and from different materials. These results demonstrate that forced air plasma treatment is a highly effective means of increasing the surface energy of polymers, which can be long-lasting, provided the treated surfaces are kept in dry conditions.
Amyotrophic Lateral Sclerosis, Parkinson’s Disease and Alzheimer’s Disease: Phylogenetic Disorders of the Human Neocortex Sharing Many Characteristics

ABSTRACT: Features common to amyotrophic lateral sclerosis (ALS), Parkinson’s disease (PD) and Alzheimer’s disease (AD) are reviewed. Shared epidemiological aspects include an increasing frequency which is proportional for each disease. We draw attention to geographic non-uniform distribution which, for ALS and PD, correlates positively with latitude. Clinical and pathological overlap occurs in the same patients, and in members of the same family. A high early morning plasma cysteine/sulphate ratio possibly related to the development of proteinacious inclusions, as well as ubiquinated neuronal inclusions, characterize ALS, PD and AD. HLA-DR (the human group II major histocompatibility class) staining is marked in ALS, PD and AD and may represent autoimmunity-incited by-products of neuronal degeneration. Based upon demonstrated glutaminergic connections between the neocortex and anterior horn cells, the entorhinal cortex and the basal ganglia we hypothesize that ALS, AD and PD are phylogenetic disturbances of the neocortical cell. The postsynaptic neuron may degenerate secondarily to anterograde effects of deranged glutamate metabolism. Future therapeutic strategies should be directed to agents that decrease transmission induced by excitatory amino-acids.