Miracle-dz/UniFAR-FTUs huggingface dataset:
Intracellular convection, homeostasis and metabolic regulation

SUMMARY Two views currently dominate experimental approaches to metabolic regulation. The first, let us call it Model 1, assumes that cells behave like a watery bag of enzymes. The alternative Model 2, however, assumes that 3-dimensional order and structure constrain metabolite behavior. A major problem in cell metabolism is determining why essentially all metabolite concentrations are remarkably stable (homeostatic) over large changes in pathway fluxes–for convenience, this is termed the [s] stability paradox. During large-scale transitions from maintenance metabolic rates to maximally activated work, contrasting demands of intracellular homeostasis versus metabolic regulation obviously arise. Data accumulated over the last 3–4 decades now make it clear that the demands of homeostasis prevail: during rest–work transitions, metabolites such as ATP and O2 are notably and rigorously homeostatic; other intermediates usually do not vary by more than 0.5- to threefold over the resting condition. This impressive homeostasis is maintained despite changes in pathway fluxes that can exceed two orders of magnitude. Classical or Model 1 approaches to this problem can explain metabolite homeostasis, but the mechanisms for each metabolite, each enzyme locus, are necessarily specific. Thus Model 1 approaches basically do not provide a global explanation for the [s] stability paradox. Model 2 takes a different tack and assumes that an intracellular convection system acts as an over-riding `assist' mechanism for facilitating enzyme–substrate encounter. Model 2 postulates that intracellular movement and convection are powered by macromolecular motors (unconventional myosins, dyneins, kinesin) running on actin or tubulin tracks. For fast and slow muscle fibers, microfilaments are concentrated near the periphery (where convection may be most important), but also extend throughout the actomyosin contractile apparatus both in horizontal and vertical dimensions. To this point in the development of the field, Model 1 and Model 2 approaches have operated as `two solitudes', each considering the other incompatible with its own experimental modus operandi. In order to finally assemble a model that can sensibly explain a realistic working range of metabolic systems, opening of channels of communication between the above two very differing views of metabolic regulation would seem to be the requirement for the future.
Parental reactions following the diagnosis of cerebral palsy in their young child.

OBJECTIVE To investigate parental reactions following the diagnosis of cerebral palsy (CP) in their young children. METHODS In this cross-sectional study, 51 parents of children with CP (mean age = 18.5 months, SD = 1.5) completed the Reaction to Diagnosis Interview, assessing their personal reactions to their children's diagnosis. Relationships between these reactions and the severity of CP in terms of motor abilities and the child's cognitive functioning, as well as coping of parents and perceived social support, were investigated using univariate and multivariate logistic regression analyses. RESULTS Thirty-nine parents (77%) were classified as "resolved. " Multivariate regression analysis revealed that severity of CP was associated with an "unresolved" status. CONCLUSION The majority of parents of children with CP have come to terms with this diagnosis by the time their children is 18 months old. The child's gross motor limitations seem to represent an important factor explaining negative parental reactions at this time.
Morphological typology of languages for IR

This paper presents a morphological classification of languages from the IR perspective. Linguistic typology research has shown that the morphological complexity of every language in the world can be described by two variables, index of synthesis and index of fusion. These variables provide a theoretical basis for IR research handling morphological issues. A common theoretical framework is needed in particular because of the increasing significance of cross‐language retrieval research and CLIR systems processing different languages. The paper elaborates the linguistic morphological typology for the purposes of IR research. It studies how the indexes of synthesis and fusion could be used as practical tools in mono‐ and cross‐lingual IR research. The need for semantic and syntactic typologies is discussed. The paper also reviews studies made in different languages on the effects of morphology and stemming in IR.
VLBA Determination of the Distance to Nearby Star-forming Regions. II. Hubble 4 and HDE 283572 in Taurus

The nonthermal 3.6 cm radio continuum emission from the naked T Tauri stars Hubble 4 and HDE 283572 in Taurus has been observed with the Very Long Baseline Array (VLBA) at six epochs between 2004 September and 2005 December with a typical separation between successive observations of 3 months. Thanks to the remarkably accurate astrometry delivered by the VLBA, the trajectory described by both stars on the plane of the sky could be traced very precisely and modeled as the superposition of their trigonometric parallax and uniform proper motion. The best fits yield distances to Hubble 4 and HDE 283572 of 132.8 ± 0.5 and 128.5 ± 0.6 pc, respectively. Combining these results with the other two existing VLBI distance determinations in Taurus, we estimate the mean distance to the Taurus association to be 137 pc with a dispersion (most probably reflecting the depth of the complex) of about 20 pc.
Genes affecting novel seed constituents in Limnanthes alba Benth: transcriptome analysis of developing embryos and a new genetic map of meadowfoam

The seed oil of meadowfoam, a new crop in the Limnanthaceae family, is highly enriched in very long chain fatty acids that are desaturated at the Δ5 position. The unusual oil is desirable for cosmetics and innovative industrial applications and the seed meal remaining after oil extraction contains glucolimnanthin, a methoxylated benzylglucosinolate whose degradation products are herbicidal and anti-microbial. Here we describe EST analysis of the developing seed transcriptome that identified major genes involved in biosynthesis and assembly of the seed oil and in glucosinolate metabolic pathways. mRNAs encoding acyl-CoA Δ5 desaturase were notably abundant. The library was searched for simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). Fifty-four new SSR markers and eight candidate gene markers were developed and combined with previously developed SSRs to construct a new genetic map for Limnanthes alba. Mapped genes in the lipid biosynthetic pathway encode 3-ketoacyl-CoA synthase (KCS), Δ5 desaturase (Δ5DS), lysophosphatidylacyl-acyl transferase (LPAT), and acyl-CoA diacylglycerol acyl transferase (DGAT). Mapped genes in glucosinolate biosynthetic and degradation pathways encode CYP79A, myrosinase (TGG), and epithiospecifier modifier protein (ESM). The resources developed in this study will further the domestication and improvement of meadowfoam as an oilseed crop.
Antimicrobial-resistant Shigella infections from Iran: an overlooked problem?

OBJECTIVES In this study, we wanted to assess the level of antimicrobial resistance, the presence of genes encoding resistance to cephalosporins and plasmid-mediated quinolone resistance (PMQR), and genetic relatedness among Shigella isolates obtained from Iranian patients. METHODS A total of 44 Shigella isolates were collected from Iranian patients admitted to Milad Hospital, Tehran, Iran, during 2008-10. Of these, 37 were serotyped and characterized by MIC determination. A subset of eight suspected extended-spectrum β-lactamase (ESBL) producers (six Shigella sonnei phase II and two Shigella flexneri type 1b) were examined for the presence of genes encoding cephalosporin resistance. The presence of PMQR was assessed in one S. flexneri isolate exhibiting low-level resistance to ciprofloxacin and susceptibility to nalidixic acid. PFGE was performed on 25 S. sonnei phase II isolates. RESULTS Of the isolates, 25 (68%) were S. sonnei phase II, with 5 (14%) S. flexneri, 5 (14%) Shigella dysenteriae type 2, and 2 (5%) Shigella boydii type 2. Resistance to at least threeclasses of antimicrobials was detected in all species. The presence of bla(CTX-M-15) and the AmpC β-lactamase producer bla(CMY-2) was confirmed in five and one S. sonnei phase II isolates, respectively. One of the two S. flexneri type 1b that contained bla(CTX-M-15) also harboured a qnrS1 gene. PFGE identified sevenPFGE profiles; the main cluster included 15 of the strains, suggesting low genetic diversity between isolates or the presence of an endemic clone in Iran. CONCLUSIONS This is the first known description of ESBL-producing and AmpC β-lactamase-producing Shigella and of PMQR Shigella in Iran. The emergence of CTX-15, CMY-2 and qnrS1 genes may compromise the treatment of shigellosis. Strategies to minimize the spread of ESBL-producing and AmpC-β-lactamase-producing Shigella should be implemented.
A Model of Packet Traffic Using a Random Wall Model

We consider the problem of modelling self-similar bursty packet traac using chaotic maps. In particular, we introduce a family of maps to overcome the diiculties of describing the self-similar statistics of the packet traac as a function of the maps' parameters. The packet traac that can be modelled with these maps varies from Poisson to bursty traac with diierent decay tails in the ON and OFF regions. In contrast with Fractional Brownian Models, these maps have a multifractal spectrum so they can generate packet traac with the same mean, variance and self-similar parameter but diierent higher order statistics.
Independent-of-delay stability of nonlinear networked control systems by scattering transformation

In this paper a novel stability condition is derived for nonlinear networked control systems (NCS) with arbitrarily large constant time delay. The proposed approach is based on input-output models of the subsystems and the scattering transformation, well-known from teleoperation systems analysis with time delay. Passivity of the subsystems has been a prerequisite for guaranteed stability in systems using the scattering transformation. In this paper, the approach is extended to non-passive, non-linear subsystems. The main idea is the compensation of the non-passivity of the plant by excess of passivity of the controller and vice-versa. A numerical example demonstrates that even unstable plants can independently of the constant delay be stabilized over the network
Differential Prediction of Immigrant Versus Majority Group Training Performance Using Cognitive Ability and Personality Measures

There are very few studies of the differential prediction of work and training performance for immigrant groups in the Netherlands. Scores on the General Aptitude Test Battery, an intelligence test, and the Amsterdamse Biografische Vragenlijst (ABV), a frequently used Dutch personality test, and training data for 78 immigrant and 78 Dutch trainee truck drivers were collected. Lautenschlager and Mendoza's (1986) method of step-down hierarchical regression analysis was used to check for bias in the prediction of training results. Some predictor-criterion combinations showed differential prediction, especially with less cognitive and less objective criteria. The results with respect to the intelligence test appear to be in line with the findings of the US literature.
Extra-Nuclear Signaling Pathway Involved in Progesterone-Induced Up-Regulations of p21cip1 and p27kip1 in Male Rat Aortic Smooth Muscle Cells

Previously, we demonstrated that progesterone (P4) at physiologic levels (5-500 nM) inhibited proliferation in cultured rat aortic smooth muscle cells (RASMCs) through a P4 receptor (PR)-dependent pathway. We also showed that P4-induced cell cycle arrest in RASMCs occurs when the cyclin-CDK2 system is inhibited just as p21cip1 and p27kip1 protein levels are augmented. In the present study, we further investigated the molecular mechanism underlying P4-induced up-regulations of p21cip1 and p27kip1 in RASMCs. We used pharmacological inhibitors as well as dominant negative constructs and conducted Western blot analyses to delineate the signaling pathway involved. Our data suggest that P4 up-regulated the expression of p21cip1 and p27kip1 in RASMCs through increasing the level of p53 protein mediated by activating the cSrc/Kras/Raf-1/AKT/ERK/p38/IκBα/NFκB pathway. The findings of the present study highlight the molecular mechanism underlying P4-induced up-regulations in p21cip1 and p27kip1 in RASMCs.
Gaze contingent joint attention with an avatar in children with and without ASD

Problems with joint attention (JA) are core features of Autism Spectrum Disorders (ASD). Here, we investigate how typically developing (TD) children and children with ASD respond to joint attention (RJA) and initiate joint attention (IJA) with a gaze contingent avatar. Thirty-one participants with ASD and 33 TD matched controls followed and directed the avatar's gaze to a series of referent images. Viewing times and recognition memory for the referent images were measured and compared between RJA and IJA conditions. Analysis of correctly identified target images suggests comparable target recognition between IJA and RJA conditions for both groups, but poorer overall recognition memory in the ASD group. Eye tracking data suggests different viewing strategies between the ASD and TD groups. These findings demonstrate the importance of considering processing time and saliency of referent objects when creating interactive social technology for children with ASD and further highlights the potential of interactive, gaze contingent social characters for enhancing our knowledge of joint attention.
A Developmental Grammar for Syllable Structure in the Production of Child Language

The order of acquisition of Dutch syllable types by first language learners is analyzed as following from an initial ranking and subsequent rerankings of constraints in an optimality theoretic grammar. Initially, structural constraints are all ranked above faithfulness constraints, leading to core syllable (CV) productions only. Subsequently, faithfulness gradually rises to the highest position in the ranking, allowing more and more marked syllable types to appear in production. Local conjunctions of Structural constraints allow for a more detailed analysis.
Using Regional Variation in Wages to Measure the Effects of the Federal Minimum Wage

The imposition of a national minimum wage standard provides a natural experiment in which the “treatment effect” varies across states depending on the fraction of workers initially earning less than the new minimum. The author exploits this fact to evaluate the effect of the April 1990 increase in the federal minimum wage on teenagers' wages, employment, and school enrollment. Comparisons of grouped and individual state data confirm that the rise in the minimum wage increased teenagers' wages. There is no evidence of corresponding losses in teenage employment or changes in teenage school enrollment.
Characterization and correction of Global Ozone Monitoring Experiment 2 ultraviolet measurements and application to ozone profile retrievals

[1] We present an assessment study of the Global Ozone Monitoring Experiment 2 (GOME-2) reflectance for the wavelength range 270–350 nm by comparing measurements with simulations calculated using the vector linearized discrete ordinate radiative transfer model (VLIDORT) and Microwave Limb Sounder (MLS) ozone profiles. The results indicate wavelength- and cross-track-position-dependent biases. GOME-2 reflectance is overestimated by 10% near 300 nm and by 15%–20% around 270 nm. Stokes fraction measurements made by onboard polarization measurement devices are also validated directly using the VLIDORT model. GOME-2 measurements agree well with the simulated Stokes fractions, with mean biases ranging from −1.0% to ∼2.9%; the absolute differences are less than 0.05. Cloudiness-dependent biases suggest the existence of uncorrected stray-light errors that vary seasonally and latitudinally. Temporal analysis indicates that reflectance degradation began at the beginning of the mission; the reflectance degrades by 15% around 290 nm and by 2.2% around 325 nm from 2007 through 2009. Degradation shows wavelength- and viewing-angle-dependent features. Preliminary validation of ozone profile retrievals with MLS, Michelson Interferometer for Passive Atmospheric Sounding, and ozonesonde reveals that the application of radiometric recalibration improves the ozone profile retrievals as well as reduces fitting residuals by 30% in band 2b.
Nonconscious processes and health.

OBJECTIVES Health behavior theories focus on the role of conscious, reflective factors (e.g., behavioral intentions, risk perceptions) in predicting and changing behavior. Dual-process models, on the other hand, propose that health actions are guided not only by a conscious, reflective, rule-based system but also by a nonconscious, impulsive, associative system. This article argues that research on health decisions, actions, and outcomes will be enriched by greater consideration of nonconscious processes. METHODS A narrative review is presented that delineates research on implicit cognition, implicit affect, and implicit motivation. In each case, we describe the key ideas, how they have been taken up in health psychology, and the possibilities for behavior change interventions, before outlining directions that might profitably be taken in future research. RESULTS Correlational research on implicit cognitive and affective processes (attentional bias and implicit attitudes) has recently been supplemented by intervention studies using implementation intentions and practice-based training that show promising effects. Studies of implicit motivation (health goal priming) have also observed encouraging findings. There is considerable scope for further investigations of implicit affect control, unconscious thought, and the automatization of striving for health goals. CONCLUSION Research on nonconscious processes holds significant potential that can and should be developed by health psychologists. Consideration of impulsive as well as reflective processes will engender new targets for intervention and should ultimately enhance the effectiveness of behavior change efforts.
Self-Repairing Disk Arrays

As the prices of magnetic storage continue to decrease, the cost of replacing failed disks becomes increasingly dominated by the cost of the service call itself. We propose to eliminate these calls by building disk arrays that contain enough spare disks to operate without any human intervention during their whole lifetime. To evaluate the feasibility of this approach, we have simulated the behavior of two-dimensional disk arrays with n parity disks and n(n-1)/2 data disks under realistic failure and repair assumptions. Our conclusion is that having n(n+1)/2 spare disks is more than enough to achieve a 99.999 percent probability of not losing data over four years. We observe that the same objectives cannot be reached with RAID level 6 organizations and would require RAID stripes that could tolerate triple disk failures.
Genetic Solutions to the Load Balancing Problem in ParallelComputers

EEcient use of resources in a parallel machine often requires the redistribution of tasks during the execution of applications. This problem of load balancing is a formidable one because of the unpredictable and dynamic nature of many user programs. In this paper, we present two load balancing methods that employ the use of genetic algorithms. While genetic algorithms and classiier systems have been used in a variety of optimization problems , this paper demonstrates their power in a domain that has been untapped. This paper also represents a departure from traditional heuristic-based load balancing schemes in that the genetic algorithm provides the primary decision-making authority. The beneets of the two approaches to genetic algorithm-based load balancing are illustrated in this paper using performance results based on a task processing simulator. In addition, the adaptability of the classiier-based system is demonstrated on two actual parallel processing machines: the Connection Machine 5 and the Intel Hypercube.
Gene expression profile of human endometrial receptivity: comparison between natural and stimulated cycles for the same patients.

BACKGROUND The adjunction of exogenous hormones for controlled ovarian stimulation (COS) may alter endometrial receptiveness. In order to identify the genes misregulated under COS, we compared the endometrium gene expression profiles, from the same patients, in a natural cycle and in a subsequent COS cycle. METHODS For the same normal-responder patients (n = 21), endometrial biopsies (n = 84) were collected during the pre-receptive (LH + 2) and receptive stages (LH + 7) of a natural cycle and, subsequently, on oocyte retrieval day (hCG + 2) and on transfer day (hCG + 5) of a stimulated cycle. Samples were analyzed using DNA microarrays. Gene expression profiles and biological pathways involved in endometrial receptivity were analyzed. RESULTS Although endometrium transition profiles from pre-receptive to receptive phases are similar between patients, COS regimens alter endometrial receptivity in comparison with natural cycle. Under COS conditions, two endometrial profiles were identified and were associated either with a moderately altered receptivity profile for the majority of the patients or a strongly altered profile for a sub-category of patients. The receptive endometrium transcription profile under COS was defective for biological functions such as TGFbeta signaling, leukocyte transendothelial migration and the cell cycle. CONCLUSIONS Gonadotrophin treatments in COS cycles led to disruptions of the transcriptional activation of genes involved in normal endometrial receptivity. We propose that when the receptiveness of the endometrium is seriously compromised by the COS protocol, fresh embryo replacement should be cancelled, the embryo frozen and thawed embryo replacement should be performed under natural cycles.
Cortical radial glia: identification in tissue culture and evidence for their transformation to astrocytes

Radial glia are transiently present in the developing cerebral cortex, where they are thought to guide the migration of neurons from the proliferative zone to the forming cortical plate. To provide a framework for experimental studies of radial glia, we have defined morphological and immunocytochemical criteria to identify them in primary cultures of cortical cells obtained at embryonic day 13 in the mouse. Cortical radial glia in culture for 1–2 d resemble radial glia in vivo: they have a long, thin, unbranched process extending from one or both ends of the elongated cell body and are labeled with the monoclonal antibody RC1 but not with antibodies to glial fibrillary acidic protein (abGFAP). We tested the specificity of RC1 by double- labeling with a panel of cell-type specific antibodies, and found that it labels radial glia, astrocytes, and fibroblast-like cells, but not neurons. Fibroblasts are easily distinguished from glia by morphology and by labeling with antibodies to fibronectin. To test the hypothesis that radial glia become astrocytes when their developmental role is complete, we examined their morphological and immunocytochemical development in culture. After 3–4 d in vitro radial glia develop several branched processes; in this transitional stage they are labeled by both RC1 and abGFAP. Many radial glia lose RC1 immunoreactivity as they become increasingly branched and immunoreactive to abGFAP. In areas of the cultures that have few neurons and in cultures depleted of neurons by washing, flat, nonprocess-bearing glia predominate. These cells do not lose immunoreactivity to RC1 during the 9-d period of observation even though they acquire GFAP. (ABSTRACT TRUNCATED AT 250 WORDS)