Miracle-dz/UniFAR-FTUs huggingface dataset:
Consort-ehealth Checklist V1.6.2 Report

Client perceptions of the Mental Health Engagement Network: A secondary analysis of a mobile and web-based intervention for individuals experiencing severe mental illness TITLE 1a-i) Identify the mode of delivery in the title "A secondary analysis of a mobile and web-based intervention for individuals experiencing severe mental illness " 1a-ii) Non-web-based components or important co-interventions in title 1a-iii) Primary condition or target group in the title "for individuals experiencing severe mental illness" ABSTRACT 1b-i) Key features/functionalities/components of the intervention and comparator in the METHODS section of the ABSTRACT "Mentalhealth clients aged 18 to 80 (n = 400) and diagnosed with a mood or psychotic disorder were provided with a smartphone (iPhone 4S) and participating care providers (n = 52) were provided with a tablet (iPad) in order to access and engage with the [Lawson SMART Record]. A delayed implementation design with mixed methods was used. Survey and interview data was collected over the course of 18 months through semi-structured interviews conducted every six months post-implementation of the intervention. " 1b-ii) Level of human involvement in the METHODS section of the ABSTRACT ". and participating care providers (n = 52) were provided with a tablet (iPad) in order to access and engage with the [Lawson SMART Record]. " 1b-iii) Open vs. closed, web-based (self-assessment) vs. face-to-face assessments in the METHODS section of the ABSTRACT "Survey and interview data was collected over the course of 18 months through semi-structured interviews conducted by experienced research assistants every six months post-implementation of the intervention. " 1b-iv) RESULTS section in abstract must contain use data "Due to drop out or loss of contact, 394 out of 400 individuals completed the study. At the end of the study 52 devices were lost or unusable. " This study involves a secondary analysis to assess clients' perceptions of using the technologies provided. Web metrics and use data (e.g., number of logins) have been reported in previous publications. 1b-v) CONCLUSIONS/DISCUSSION in abstract for negative trials "Quantitative and qualitative findings from this analysis demonstrated that these technologies positively impacted the lives of individuals experiencing severe mental illnesses and dispelled some of the myths regarding retention of technology among marginalized populations. This secondary analysis supported the acceptability and applicability of using mental health technologies within this population and provided considerations for future development. " INTRODUCTION 2a-i) Problem and the type of system/solution "Healthcare systems and agencies have increasingly invested in information technology to improve the quality and …
Deciphering the reading of the genetic code by near-cognate tRNA

Significance Protein translation is a key cellular process in which each codon of mRNAs has to be accurately and efficiently recognized by cognate tRNAs of a large repertoire of noncognate tRNAs. A successful decoding process is largely dependent on the presence of modified nucleotides within the anticodon loop, especially of tRNAs having to read A/U-rich codons. In this latter case, their roles appear to stabilize the codon–anticodon interaction, allowing them to reach an optimal energetic value close to that of other interacting tRNAs involving G/C-rich anticodons. In this work we demonstrate that, while helping an efficient translation of A/U-rich codons, modified nucleotides also allow certain unconventional base pairing to occur, as evidenced in the case of stop codon suppression. Some codons of the genetic code can be read not only by cognate, but also by near-cognate tRNAs. This flexibility is thought to be conferred mainly by a mismatch between the third base of the codon and the first of the anticodon (the so-called “wobble” position). However, this simplistic explanation underestimates the importance of nucleotide modifications in the decoding process. Using a system in which only near-cognate tRNAs can decode a specific codon, we investigated the role of six modifications of the anticodon, or adjacent nucleotides, of the tRNAs specific for Tyr, Gln, Lys, Trp, Cys, and Arg in Saccharomyces cerevisiae. Modifications almost systematically rendered these tRNAs able to act as near-cognate tRNAs at stop codons, even though they involve noncanonical base pairs, without markedly affecting their ability to decode cognate or near-cognate sense codons. These findings reveal an important effect of modifications to tRNA decoding with implications for understanding the flexibility of the genetic code.
Mediterranean Diet Supplemented With Coenzyme Q10 Modulates the Postprandial Metabolism of Advanced Glycation End Products in Elderly Men and Women

Advanced glycation end products (AGEs) and oxidative stress are elevated with aging and dysmetabolic conditions. Because a Mediterranean (Med) diet reduces oxidative stress, serum AGEs levels, and gene expression related to AGEs metabolism in healthy elderly people, we studied whether supplementation with coenzyme Q10 (CoQ) was of further benefit. Twenty participants aged ≥ 65 (10 men and 10 women) were randomly assigned to each of three isocaloric diets for successive periods of 4 weeks in a crossover design: Med diet, Med + CoQ, and a Western high-saturated-fat diet (SFA diet). After a 12-hour fast, volunteers consumed a breakfast with a fat composition similar to the previous diet period. Analyses included dietary AGEs consumed, serum AGEs and AGE receptor-1 (AGER1), receptor for AGEs (RAGE), glyoxalase I (GloxI), and estrogen receptor α (ERα) mRNA levels. Med diet modulated redox-state parameters, reducing AGEs levels and increasing AGER1 and GloxI mRNA levels compared with the SFA diet. This benefit was accentuated by adding CoQ, in particular, in the postprandial state. Because elevated oxidative stress/inflammation and AGEs are associated with clinical disease in aging, the enhanced protection of a Med diet supplemented with CoQ should be assessed in a larger clinical trial in which clinical conditions in aging are measured.
Teaching Language to Deaf Infants with a Robot and a Virtual Human

Children with insufficient exposure to language during critical developmental periods in infancy are at risk for cognitive, language, and social deficits [55]. This is especially difficult for deaf infants, as more than 90% are born to hearing parents with little sign language experience [48]. We created an integrated multi-agent system involving a robot and virtual human designed to augment language exposure for 6-12 month old infants. Human-machine design for infants is challenging, as most screen-based media are unlikely to support learning in [33]. While presently, robots are incapable of the dexterity and expressiveness required for signing, even if it existed, developmental questions remain about the capacity for language from artificial agents to engage infants. Here we engineered the robot and avatar to provide visual language to effect socially contingent human conversational exchange. We demonstrate the successful engagement of our technology through case studies of deaf and hearing infants.
Metallothionein Prevents Age-Associated Cardiomyopathy via Inhibiting NF-κB Pathway Activation and Associated Nitrative Damage to 2-OGD.

AIMS Cardiac-specific metallothionein (MT) overexpression extends lifespan, but the mechanism underlying the effect of MT protection against age-associated cardiovascular diseases (CVD) remains elusive. To elucidate this, male wild-type and two lines of MT-transgenic (MT-TG) mice, MM and MT-1 (cardiac-specific overexpressing MT about 10- and 80-fold, respectively) at three representative ages (2-3, 9-10, and 18-20 months), were utilized. A stable human MT2A overexpressing cardiomyocytes (H9c2MT7) was also introduced. RESULTS Histomorphology and echocardiographic analysis revealed that age-associated cardiac hypertrophy, remodeling, and dysfunction were ameliorated in MT-TG mice. Also, aging-accompanied NF-κB activation, characterized by increased nuclear p65 translocation, elevated DNA-binding activity, and upregulation of inflammatory cytokines, was largely attenuated by MT overexpression. Treatment of H9c2 cardiomyocytes with tumor necrosis factor-α (TNF-α), which mimicked an inflammatory environment, significantly increased NF-κB activity, and some age-related phenotypes appeared. The NF-κB activation was further proved to be pivotal for both age-associated and TNF-α-induced nitrative damage to cardiac 2-oxoglutarate dehydrogenase (2-OGD) by virtue of NF-κB p65 gene silencing. MT inhibited NF-κB activation and associated nitrative damage to cardiac 2-OGD in both old MT-TG hearts and TNF-α-treated H9c2MT7 cardiomyocytes; these protective effects were abolished in H9c2MT7 cardiomyocytes by MT-specific gene silencing. Innovation and Conclusion: Together, these findings indicate that the protective effects of MT against age-associated CVD can be attributed mainly to its role in NF-κB inhibition and resultant alleviation of nitrative damage to 2-OGD. Antioxid. Redox Signal. 25, 936-952.
Dual synchronization of chaotic and hyperchaotic systems

The existence of the dual synchronization behavior between a pair of chaotic and hyperchaotic systems is investigated via a nonlinear controller, in which the nonlinear functions of the system are used as a nonlinear feedback term. The sufficient conditions for achieving the dual synchronization behavior between a pair of chaotic and hyperchaotic systems using a nonlinear feedback controller are derived by using the Lyapunov stability theorem. The dual synchronization behavior between a pair of chaotic systems (Chen and Lorenz system) and a pair of hyperchaotic systems hyperchaotic Chen system and hyperchaotic Lü system are taken as two illustrative examples to show the effectiveness of the proposed method. Theoretical analysis and numerical simulations are performed to verify the results. c ©2016 All rights reserved.
Effectiveness, Safety, and Costs of a Treatment Switch to Dolutegravir Plus Rilpivirine Dual Therapy in Treatment-Experienced HIV Patients

Background: Evidence about the use of dolutegravir (DTG) and rilpivirine (RPV) as an antiretroviral therapy (ART) in treatment-experienced patients is scarce. Objective: To explore the effectiveness, safety, and costs of switching to a DTG plus RPV regimen in this population. Methods: This observational, prospective study included all treatment-experienced patients who switched to DTG plus RPV between November 2014 and July 2016. Patients were excluded if resistance mutations to integrase inhibitors or RPV were found. The effectiveness endpoint was the proportion of patients who achieved virological suppression (viral load [VL] <50 copies/mL) at week 48 (W48). Safety (incidence of adverse events leading to discontinuation and laboratory abnormalities), adherence, and costs were analyzed. Results: A total of 35 patients were included, and 91.4% were virologically suppressed at baseline. Patients were treated with ART for a median of 14 years (interquartile range = 7-20). At W48, 91.4% of patients were virologically suppressed (95% CI = 77.0-98.2). Two of the 3 patients not suppressed at baseline achieved undetectable VL at W48, and 2 patients discontinued DTG plus RPV (intolerance and a drug-drug interaction). None of the virologically suppressed patients at baseline showed virological rebound through W48. There were no significant changes in lipid, liver, and renal profiles. The proportion of patients with an ART adherence >90% increased from 65.6% to 93.8% (P = 0.004). The annual per-patient ART costs dropped by €665 (P = 0.265). Conclusions: Switching to DTG plus RPV seems to be an effective and safe strategy. Significant improvements in patients’ adherence and costs were achieved.
Holocene elephant seal distribution implies warmer-than-present climate in the Ross Sea

We show that southern elephant seal (Mirounga leonina) colonies existed proximate to the Ross Ice Shelf during the Holocene, well south of their core sub-Antarctic breeding and molting grounds. We propose that this was due to warming (including a previously unrecognized period from ≈1,100 to 2,300 14C yr B.P.) that decreased coastal sea ice and allowed penetration of warmer-than-present climate conditions into the Ross Embayment. If, as proposed in the literature, the ice shelf survived this period, it would have been exposed to environments substantially warmer than present.
Encephalitis after influenza and vaccination: a nationwide population-based registry study from Norway

Abstract Background Influenza is known to be associated with various neurological complications, including encephalitis. We conducted a registry-based study to assess the risk of encephalitis after influenza and A(H1N1)pdm09 vaccine. Methods Data from Norwegian national health registries during 2008–14 were linked using the unique personal identifiers given to all Norwegian residents (N = 5 210 519). Cox proportional-hazard models with time-varying variables were fitted to estimate hazard ratios (HRs) of encephalitis after influenza and A(H1N1)pdm09 vaccine, using the risk windows 0–7, 0–14, 0–30, 0–60, 0–90 and 0–180 days. Results In Norway, 684 172 individuals received an influenza diagnosis and 2793 patients were hospitalized with encephalitis during 2008–14. The risk of encephalitis increased after influenza: HR, 7-day risk window: 47.8 (95% confidence interval (CI): 35.8–63.8), and the HR decreased for longer risk windows; HR, 180-day risk window: 3.8 (95% CI: 3.1–4.7). HR of encephalitis after influenza during the 2009 main pandemic wave using a 7-day risk window was 30.0 (95% CI: 10.8–83.2). We found no differences in the risk of encephalitis after the seasonal influenza compared with influenza during the 2009 main pandemic wave; HR, 7-day risk window: 1.3 (95% CI: 0.4–4.3). A(H1N1)pdm09 vaccine was not associated with the risk of encephalitis: HR, 14-day risk window: 0.6 (95% CI: 0.2–2.1). Conclusions There was an increased risk of encephalitis following influenza but not after A(H1N1)pdm09 vaccine. The risk of encephalitis was highest in the first few weeks after influenza.
Cathepsin S contributes to microglia‐mediated olfactory dysfunction through the regulation of Cx3cl1–Cx3cr1 axis in a Niemann–Pick disease type C1 model

Microglia can aggravate olfactory dysfunction by mediating neuronal death in the olfactory bulb (OB) of a murine model of Niemann–Pick disease type C1 (NPC1), a fatal neurodegenerative disorder accompanied by lipid trafficking defects. In this study, we focused on the crosstalk between neurons and microglia to elucidate the mechanisms underlying extensive microgliosis in the NPC1‐affected brain. Microglia in the OB of NPC1 mice strongly expressed CX3C chemokine receptor 1 (Cx3cr1), a specific receptor for the neural chemokine C‐X3‐C motif ligand 1 (Cx3cl1). In addition, a high level of Cx3cl1 was detected in NPC1 mouse‐derived CSF due to enhanced catalytic activity of Cathepsin S (Ctss), which is responsible for Cx3cl1 secretion. Notably, nasal delivery of Cx3cl1 neutralizing antibody or Ctss inhibitor could inhibit the Cx3cl1–Cx3cr1 interaction and support neuronal survival through the suppression of microglial activation, leading to an improvement in the olfactory function in NPC1 mice. Relevant in vitro experiments revealed that intracellular cholesterol accumulation could act as a strong inducer of abnormal Ctss activation and, in turn, stimulated the Cx3cl1–Cx3cr1 axis in microglia via p38 mitogen‐activated protein kinase signaling. Our data address the significance of Cx3cl1–Cx3cr1 interaction in the development of microglial neurotoxicity and suggest that Ctss is a key upstream regulator. Therefore, this study contributes to a better understanding of the crosstalk between neurons and microglia in the development of the neurodegeneration and provides a new perspective for the management of olfactory deficits and other microglia‐dependent neuropathies. GLIA 2016;64:2291–2305
Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

In vivo corneal confocal microscopy allows the examination of each layer of the cornea in detail and the identification of pathological changes at the cellular level. The purpose of this study was to identify the possible effects of a three‐month treatment with autologous serum eye‐drops in different corneal layers of patients with severe dry eye disease using corneal confocal microscopy.
Communicating the nutritional value of sugar in Drosophila

Significance Fruit flies release a previously unreported signal when they consume nutritive food. This signal communicates the presence of nutritive food to other flies and guides them to aggregate around the nutritive food. The nutritional value of food, rather than its taste, is critical for the release of calorie-induced secreted factor (CIF) from the gut end. This signal is not detected by the olfactory system and is not limited to one species in Drosophila. We propose that CIF acts as an ethologically important pheromone that informs nutritional food sources to other flies in the wild. Sweet-insensitive Drosophila mutants are unable to readily identify sugar. In presence of wild-type (WT) flies, however, these mutant flies demonstrated a marked increase in their preference for nutritive sugar. Real-time recordings of starved WT flies revealed that these flies discharge a drop from their gut end after consuming nutritive sugars, but not nonnutritive sugars. We proposed that the drop may contain a molecule(s) named calorie-induced secreted factor (CIF), which serves as a signal to inform other flies about its nutritional value. Consistent with this, we observed a robust preference of flies for nutritive sugar containing CIF over nutritive sugar without CIF. Feeding appears to be a prerequisite for the release of CIF, given that fed flies did not produce it. Additionally, correlation analyses and pharmacological approaches suggest that the nutritional value, rather than the taste, of the consumed sugar correlates strongly with the amount (or intensity) of the released CIF. We observed that the release of this attractant signal requires the consumption of macronutrients, specifically nutritive sugars and l-enantiomer essential amino acids (l-eAAs), but it is negligibly released when flies are fed nonnutritive sugars, unnatural d-enantiomer essential amino acids (d-eAAs), fatty acids, alcohol, or salts. Finally, CIF (i) is not detected by the olfactory system, (ii) is not influenced by the sex of the fly, and (iii) is not limited to one species of Drosophila.
Using the Wild Bootstrap to Quantify Uncertainty in DTI

Estimation of noise-induced variability in diffusion tensor imaging (DTI) is needed to objectively follow disease progression, in therapeutic monitoring and to provide consistent readouts of pathophysiology. The noise variability of non-linear quantities of the diffusion tensor (e.g., fractional anisotropy, fiber orientation, etc.) have been quantified using the bootstrap, in which the data are resampled from the experimental averages, yet this approach is only applicable to DTI scans that contain multiple averages from the same sampling direction. It has been shown that DTI acquisitions with a modest to large number of directions, in which each direction is only sampled once, outperform the multiple averages approach. These acquisitions resist the traditional (regular) bootstrap analysis though. In contrast to the regular bootstrap, the wild bootstrap method can be applied to such protocols in which there is only one observation per direction. Here, we compare and contrast the wild bootstrap with the regular bootstrap using Monte Carlo numerical simulations for a number of diffusion scenarios. The regular and wild bootstrap methods are applied to human DTI data and empirical distributions are obtained for fractional anisotropy and the diffusion tensor eigensystem. Spatial maps of the estimated variability in the diffusion tensor principal eigenvector are provided. The wild bootstrap method can provide empirical distributions for tensor-derived quantities, such as fractional anisotropy and principal eigenvector direction, even when the exact distributions are not easily derived.
Testing QoE in Different 3D HDTV Technologies

The three dimensional (3D) display technology has started flooding the consumer television market. There are a number of different systems available with different marketing strategies and different advertised advantages. The main goal of the experiment described in this paper is to compare the systems in terms of achievable Quality of Experience (QoE) in different situations. The display systems considered are the liquid crystal display using polarized light and passive lightweight glasses for the separation of the leftand right-eye images, a plasma display with time multiplexed images and active shutter glasses and a projection system with time multiplexed images and active shutter glasses. As no standardized test methodology has been defined for testing of stereoscopic systems, we develop our own approach to testing different aspects of QoE on different systems without reference using semantic differential scales. We present an analysis of scores with respect to different phenomena under study and define which of the tested aspects can really express a difference in the performance of the considered display technologies.
Celiac antibodies in children with type 1 diabetes – A diagnostic validation study

Abstract Introduction: Autoimmune diseases, such as celiac disease (CD) and diabetes mellitus type 1, tend to co-occur within the same patient. The prevalence of CD in diabetic children is higher than in the general population, and is estimated to be 0.6–16.4%. The diagnosis of CD is based on histopathological examination and serological testing, however, these methods are still imperfect and new diagnostic algorithms should be considered. Aim: The aim of the study was to assess the diagnostic value of serological tests detecting antibodies against deamidated gliadin peptide, endomysium, tissue transglutaminase, neo-epitope tissue transglutaminase and to identify HLA-related genetic predisposition to CD in patients with type 1 diabetes mellitus (DM1). Methods: Autoantibodies were measured in the sera of 392 children suffering from DM1 aged 1–19 years old (mean 11.76 ± 4.14 years old). Additionally, PCR-based assessment of HLA DQ2/DQ8 genotyping was performed. Results: A positive result of at least one serological test was obtained from 81 children (20.66%). The sensitivity and specificity were 76.47% and 91.67% for anti-DGP IgA, 70.59% and 58.33% for IgG anti-DGP, respectively. A positive predictive value was 100% for the anti-TG IgA at cutoff levels of 5 and 10 times higher than upper limit of reference values. HLA DQ2 and/or DQ8 were found in 97.6% of examined children. Conclusions: Tests based on anti-TG IgA are more accurate for detecting CD in children with type 1 diabetes than anti-DGP IgA. A high percentage of diabetic children carry HLA alleles predisposing to CD, which indicates that genetic screening in this group of patients is not obligated.