Miracle-dz/UniFAR-FTUs huggingface dataset:
Caenorhabditis elegans Genes Required for the Engulfment of Apoptotic Corpses Function in the Cytotoxic Cell Deaths Induced by Mutations in lin-24 and lin-33

Two types of cell death have been studied extensively in Caenorhabditis elegans, programmed cell death and necrosis. We describe a novel type of cell death that occurs in animals containing mutations in either of two genes, lin-24 and lin-33. Gain-of-function mutations in lin-24 and lin-33 cause the inappropriate deaths of many of the Pn.p hypodermal blast cells and prevent the surviving Pn.p cells from expressing their normal developmental fates. The abnormal Pn.p cells in lin-24 and lin-33 mutant animals are morphologically distinct from the dying cells characteristic of C. elegans programmed cell deaths and necrotic cell deaths. lin-24 encodes a protein with homology to bacterial toxins. lin-33 encodes a novel protein. The cytotoxicity caused by mutation of either gene requires the function of the other. An evolutionarily conserved set of genes required for the efficient engulfment and removal of both apoptotic and necrotic cell corpses is required for the full cell-killing effect of mutant lin-24 and lin-33 genes, suggesting that engulfment promotes these cytotoxic cell deaths.
Time Domain Formulation of Pulse-Doppler Ultrasound and Blood Velocity Estimation by Cross Correlation

The Doppler effect is usually described as a frequency shift of the backscattered signals from moving targets with respect to the frequency transmitted. Recently, real-time blood flow imaging has become possible thanks to the development of a new velocity estimator based on phase-shift measurements of successive echoes. However, this method suffers from the well-known limitations of pulse-Doppler instruments. A new formulation is presented which describes the pulse-Doppler effect on the successive echoes from a cloud of moving targets as a progressive translation in time due to the displacement of the scatterers between two excitations. This approach allows us to generate efficiently computer-simulated data in order to evaluate accurately the various processing techniques. Furthermore, it leads to a novel class of velocity estimators in the time domain which measure the time shifts which are proportional to the local blood velocity. Among them, the cross correlation of the received rf signals turns out to be well suited. A local cross-correlation function is first calculated from a consecutive pair of range-gated echoes and the time shift is then determined by searching for the time position with the maximum correlation. The time-correlation technique is shown to provide accurate velocity profiles with broadband transducers. Moreover, the classical velocity limitation of pulse-Doppler is overcome because there is no ambiguity in measuring a time shift instead of a phase shift. These major advantages should make quantitative flow mapping possible and more reliable.
Recanalization and reperfusion in acute ischemic stroke

Revascularization remains the most intuitive strategy to reverse ischemic injury associated with arterial occlusion in acute stroke. Revascularization may lead to opening of an occluded artery, or recanalization, yet restoration of downstream flow, or reperfusion, may not ensue. Revascularization strategies and novel devices continue to broaden options for the treatment of acute stroke, but it is increasingly apparent that selection criteria to identify ideal cases are needed to refine triage and minimize adverse events. The results of recent work on reperfusion may rapidly alter routine clinical practice for evolving ischemia in the brain.
Inverted Meckel’s Diverticulum: Rare Etiology of an Intestinal Obstruction

Acute gastrointestinal obstruction can have a varied spectrum of clinical presentation and etiologies. It has been studied in detail and the management criteria have been well defined for the most part in our era. The etiologies are usually well defined. However, acute small bowel obstruction (SBO) due to intussusception caused by an inverted Meckel's diverticulum is a rare phenomenon that is often times missed on initial presentation and/or consequently until resected and visualized on pathological examination. We present a case of a 34-year-old presenting with symptoms and signs of acute intestinal obstruction and radiographic exam showing ileo-ileal intussusception. The patient failed to improve initially following conservative management and was taken to the operating room for small bowel resection which then revealed an inverted Meckel’s diverticulum.
Distribution of flourescently labeled α-actinin in living and fixed fibroblasts

The distribution of flourescently labeled α-actinin after microinjection into fibroblasts has been determined in both living and fixed cells. We have found that the distribution of the injected tetramethylrhodamine isthiocyanate-labeled protein (TMRITC-α-actinin) in living cells, which is in ruffling membranes, actin microfilament bundles, and polygonal microfilament networks (Feramisco, 1979, Proc. Natl. Acad. Sci. U. S. A. 76:3967-3971), was virtually unaffected by the fixation (3.5 percent formaldehyde) and extraction (absolute acetone) used for the preparation of the cells for immunoflourescence. Also, these patterns were found to coincide with the α-actinin revealed by immunoflourescence. Also, these patterns were found to coincide with the α-actinin revealed by immunoflourescence. These findings offer, for the first time, evidence indicating the validity of the immunoflourescence technique in the localization of α-actinin in cultured cells. With the combination of the injection procedure and the immunoflourescence localization of endogenous structural proteins, it was determined that nearly all of the actin stress fibers were decorated in a periodic manner with the injected α-actinin. Endogenous tropomyosin in the injected cells was found to be distributed with a periodic pattern along the stress fibers that was antiperiodic to the pattern observed for the microinjected α-actinin. The tropomyosin antibody stained the polygonal microfilament networks and was excluded from the foci, whereas the microinjected α-actinin was incorporated into the foci of the networks. Thus, the microinjected fluorescent derivative of α-actinin appears to be incorporated into the functional pools of α-actinin within the living cell and to be utilized by the cell with fidelity.
Evaluation of the Effectiveness of Peer Education in Improving HIV Knowledge, Attitude, and Sexual Behaviours among In-School Adolescents in Osun State, Nigeria

Introduction. Young people are at the centre of the global HIV/AIDS epidemic. This study therefore aimed to evaluate the effectiveness of peer education in improving HIV knowledge, attitude, and preventive practices among in-school adolescents in Osun State, Nigeria. Methods. This was an intervention study that was carried out among in-school adolescents attending mixed secondary schools in Osun State, Nigeria. The study was in three stages: before intervention, intervention, and after intervention. The impact of peer education was evaluated twelve weeks after intervention. Data were collected using pretested semistructured questionnaires and data analysis was done with SPSS version 16. Results. At the preintervention stage, the study and control groups were similar in their sociodemographic characteristics, HIV knowledge, attitude, and preventive practices, including high risk behaviours for HIV/AIDS transmission. After the peer education intervention, those with good knowledge and positive attitudes towards HIV/AIDS increased significantly from 50.0% to 86.7% and from 49.0% to 85.6%, respectively (P < 0.05). Conclusion. The study showed that peer education is effective in improving knowledge, attitude, and some preventive practices towards HIV/AIDS among in-school adolescents. Educational programmes about HIV/AIDS should therefore be designed to target this age group putting into consideration their unique characteristics.
Affect Convergence in Groups: The Role of Group Composition

The study of emotions is an important area to consider within a workplace context, as the experience of emotions has been linked to employees' psychological outcomes and performance. This is especially important in a group context, as individuals working within a group can converge emotionally, producing a group affective tone. Models describing the emergence of group affective tone have generally ignored the role of group diversity. We develop a model which considers how the diversity of a group (in terms of personality) influences the development of groups' affective tone, through its impact on group identification, and also specify the impact of group affective tone on group performance. Practical applications of the model for a workplace context are discussed.
Imaging evalution of the gingival fibromatosis and dental abnormalities syndrome.

OBJECTIVE The purpose of this study was to evaluate the dentomaxillofacial imaging features of one family affected by the gingival fibromatosis (GF) and dental abnormalities (DA) syndrome. METHODS Conventional radiographs (periapical and panoramic) and cone beam CT (CBCT) were performed in nine members of this family: four were affected by the syndrome and five were not. RESULTS The four affected members demonstrated mild generalized GF in association with DA, including hypoplastic amelogenesis imperfecta, intrapulpal calcifications, delay on tooth eruption and pericoronal radiolucencies in unerupted teeth. None of these oral changes were identified in the five unaffected members. All nine members presented alterations in the paranasal sinuses and mucosal thickening of the maxillary sinus was the most common finding. CONCLUSION Family members not affected by the syndrome showed similar alterations in the paranasal sinuses and CBCT was useful to characterize the dentomaxillofacial features of this new syndrome associating GF and DA.
Evidence for metallicity spreads in three massive M 31 globular clusters

Aims. We quantify the intrinsic width of the red giant branches of three massive globular clusters in M 31 in a search for metallicity spreads within these objects. Methods. We present HST/ACS observations of three massive clusters in M 31, G78, G213, and G280. A thorough description of the photometry extraction and calibration is presented. After derivation of the color-magnitude diagrams, we quantify the intrinsic width of the red giant branch of each cluster. Results. This width translates into a metallicity dispersion that indicates a complex star formation history for this type of system. For G78, σ[Fe/H] = 0.86 ± 0.37; for G213, 0.89 ± 0.20; and for G280, 1.03 ± 0.26. We find that the metallicity dispersion of the clusters does not scale with mean metallicity. We also find no trend with the cluster mass. We discuss some possible formation scenarios that would explain our results.
The dynamic consultation

This book introduces a unique model of medical discourse that identifies the forms of talk – voices – that doctors and patients use during the consultation, and studies the dynamic interaction as it unfolds particularly in follow-up visits. Natural recordings, semi-structured interviews, questionnaires and ethnographic observations provide the data for the research, which was carried out in an Outpatient Clinic in Santiago, Chile. Using an interactional sociolinguistic approach, analysis of the data identifies doctor–patient communication as a micro-performance of broader socio-cultural realities, in which social status, power, knowledge and personal beliefs and values all find expression in the consultative setting. Importantly, while both doctor and patient voices are shown to contribute to an essentially asymmetrical exchange, the study also identifies the holistic and empathic Fellow Human voice , which places doctors and patients on a more equal footing. In connection with this voice , the Spanish concept of simpatia is also discussed.While the model in this study was developed within a specific socio-cultural framework, it is hoped that it will be adapted and modified more widely and contribute to a better understanding between doctors and their patients.
Diagnosis and treatment of worker-related musculoskeletal disorders of the upper extremity.

ion forms with similar design were created to contain information on treatment outcomes. Separate abstraction forms were needed for dichotomous, categorical and continuous outcome data. These forms contained entries for the patient group identification number, number of patients reporting the outcomes, and time the outcome was measured. A separate record was entered for each patient group and each follow up time for which an outcome was reported. Special forms were designed for symptoms, comorbidities, complications, and results of diagnostic tests. Because diagnostic trials differ from treatment trials in many important ways, several special forms were used in the abstraction of diagnostic data, and irrelevant sections of the other data abstraction forms were not completed. One clinical trial information form and one diagnostic clinical trial information form were completed for each study; not all of the fields in the clinical trial information form were relevant to the diagnostic studies. One patient groups—diagnostics and characteristics form was completed for each patient group or subgroup in each study. Most articles from which we abstracted data reported on two groups; some reported more. One diagnostic test information form was completed for each diagnostic test result
Call centre employees and tobacco dependence: making a difference.

CONTEXT India is known as the Business Process Outsourcing (BPO) capital of the world. Safeguarding health of millions of youngsters employed in this new growing economy is an occupational health challenge. AIMS This study was initiated in June 2007 in India with the objectives to assess the prevalence of tobacco use and study the factors responsible for initiating and continuing its use. The main aim, however, was to assess the effect of different tobacco cessation intervention strategies, thus identifying effective methods to assist these employees to quit tobacco. MATERIALS AND METHODS This is a 4-arm cluster randomized trial of 18 months duration among 646 BPO employees, working in 4 different BPO units. The employees were invited to participate in interviews following which tobacco users of each BPO were offered specific tobacco cessation interventions to assist them to quit tobacco use. RESULTS The prevalence of tobacco dependence is 41%, mainly cigarette smoking. The tobacco quit rate is similar (nearly 20%) in the 3 intervention arms. Significantly higher reduction in tobacco consumption of 45% is seen in Arm 4 with the use of pharmacotherapy. BPO employees change jobs frequently, hence follow-up remains a major challenge. CONCLUSION Inaccessibility of pharmacotherapy in the developing countries should not deter tobacco cessation efforts as good tobacco quit rates can be achieved with health education and behavioral therapy. Tobacco cessation should be an integral activity in all BPOs, so that the employees receive this service continuously and millions of our youths are protected from the hazards of tobacco.
Valois, a component of the nuage and pole plasm, is involved in assembly of these structures, and binds to Tudor and the methyltransferase Capsuléen

Using the Capsuléen (Csul) methyltransferase as bait in the yeast two-hybrid system, we have identified a novel Drosophila protein containing multiple WD repeats and encoded by the valois (vsl) gene, which acts in pole plasm function. Vls is homologous to human MEP50, which forms a complex with the PRMT5 methyltransferase – the human homologue of Csul. We found that Vls localizes to the nuage in the nurse cells and to the pole plasm in the oocyte. Moreover vls is required for the synthesis and/or stability of Oskar and the localization of Tudor (Tud) in both the nuage and at the posterior pole of the oocyte. Furthermore, we show that Vls and a fragment of Tud interact directly in binding assay. As the PMRT5/MEP50 complex is involved in ribonucleoprotein complex assembly, we hypothesize that the Vls complex may play a similar function in assembling the nuage in nurse cells and the polar granules in the oocyte.
Children's Play and Early Childhood Education: Insights from History and Theory

Play has long been a significant part of early childhood education programs. How play is used in programs today, as well as how it is regarded, often relates to how play has been conceived at various times in the past. Understanding how play has been interpreted throughout history and how educators and psychologists view play today can help teachers of young children better understand the nature of play and how to use it in early childhood programs. This article reviews the history of the portrayal of play in art and literature, and both the classical and contemporary theories of play.
Cloning and expression of the gene encoding the diapause hormone and pheromone biosynthesis activating neuropeptide of the beet armyworm, Spodoptera exigua

Diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN), two important insect neuropeptides, regulate insect development and sex pheromone biosynthesis, respectively. DH-like immunoreactivity has been detected in the suboesophageal ganglion (SG) of pharate adult of Spodoptera exigua (Spe) by using an antiserum against Helicoverpa armigera DH. A full-length of Spe-DH-PBAN cDNA was obtained based on reverse transcription-PCR and rapid amplification of cDNA ends strategies. The open reading frame of this cDNA encodes a 197-amino acid precursor protein that contains DH, PBAN, and three other SG neuropeptides, all of which share a conservative C-terminal pentapeptide motif FXPR/KL (X = G, T or S). Northern blot analysis demonstrates the presence of an 800 bp transcript in the SG. The Spe-DH-PBAN mRNA is detectable at high levels at larval and adult stages, suggesting that Spe-DH-PBAN gene might be correlated with larval development and sex pheromone biosynthesis in moths.
Functional Genomic Analysis of Candida albicans Adherence Reveals a Key Role for the Arp2/3 Complex in Cell Wall Remodelling and Biofilm Formation

Fungal biofilms are complex, structured communities that can form on surfaces such as catheters and other indwelling medical devices. Biofilms are of particular concern with Candida albicans, one of the leading opportunistic fungal pathogens of humans. C. albicans biofilms include yeast and filamentous cells that are surrounded by an extracellular matrix, and they are intrinsically resistant to antifungal drugs such that resolving biofilm infections often requires surgery to remove the contaminated device. C. albicans biofilms form through a regulated process of adhesion to surfaces, filamentation, maturation, and ultimately dispersion. To uncover new strategies to block the initial stages of biofilm formation, we utilized a functional genomic approach to identify genes that modulate C. albicans adherence. We screened a library of 1,481 double barcoded doxycycline-repressible conditional gene expression strains covering ~25% of the C. albicans genome. We identified five genes for which transcriptional repression impaired adherence, including: ARC18, PMT1, MNN9, SPT7, and orf19.831. The most severe adherence defect was observed upon transcriptional repression of ARC18, which encodes a member of the Arp2/3 complex that is involved in regulation of the actin cytoskeleton and endocytosis. Depletion of components of the Arp2/3 complex not only impaired adherence, but also caused reduced biofilm formation, increased cell surface hydrophobicity, and increased exposure of cell wall chitin and β-glucans. Reduced function of the Arp2/3 complex led to impaired cell wall integrity and activation of Rho1-mediated cell wall stress responses, thereby causing cell wall remodelling and reduced adherence. Thus, we identify important functional relationships between cell wall stress responses and a novel mechanism that controls adherence and biofilm formation, thereby illuminating novel strategies to cripple a leading fungal pathogen of humans.
Simulating Retrieval from a Highly Clustered Network: Implications for Spoken Word Recognition

Network science describes how entities in complex systems interact, and argues that the structure of the network influences processing. Clustering coefficient, C – one measure of network structure – refers to the extent to which neighbors of a node are also neighbors of each other. Previous simulations suggest that networks with low C dissipate information (or disease) to a large portion of the network, whereas in networks with high C information (or disease) tends to be constrained to a smaller portion of the network (Newman, 2003). In the present simulation we examined how C influenced the spread of activation to a specific node, simulating retrieval of a specific lexical item in a phonological network. The results of the network simulation showed that words with lower C had higher activation values (indicating faster or more accurate retrieval from the lexicon) than words with higher C. These results suggest that a simple mechanism for lexical retrieval can account for the observations made in Chan and Vitevitch (2009), and have implications for diffusion dynamics in other fields.
Epithelial Markers to Detect Occult Microinvasion in Serous Ovarian Tumors

Summary:Ovarian serous lesions with severe cytological and architectural atypia without obvious destructive stromal invasion may be diagnosed as serous borderline malignant tumor or as serous cystadenocarcinoma, depending on the criteria that individual pathologists use. The recent introduction of the diagnosis “serous borderline tumors with stromal microinvasion” seems an important improvement for the accuracy of the diagnosis of serous ovarian tumors. The aim of this study was to determine if immunohistochemical epithelial markers could help to detect stromal microinvasion in serous ovarian tumors and to compare these findings with the occurrence of “eosinophilic metaplastic” cells. Therefore, we studied the presence of eosinophilic metaplastic cells. Three immunohistochemical epithelial markers were applied in a group of 42 borderline and invasive serous tumors. The histopathologic diagnosis of the tumors was established by a reference center for gynecologic pathology in the Netherlands. We found that “eosinophilic metaplastic” cells were a constant feature in the serous borderline tumor lesions both with and without microinvasion. The presence of these cells should therefore not be considered as pathognomonic for microinvasion. The three investigated antibodies against epithelial epitopes helped to detect microinvasion, with the monoclonal antikeratin (CAM5.2) the best of these antibodies. Serous tumors diagnosed as carcinoma with dubious invasion showed no evidence of micro- invasion in 83% of cases. In 13% of serous borderline malignant tumors, microinvasion was detected by the antibodies. It is proposed that, in cases of atypical serous ovarian tumors, a panel of CAMS.2 with other antibodies such as epithelial membrane antigen (EMA) and/or polyclonal keratin (pKer) be applied on tissue sections with the highest degree of cytological and architectural atypia to detect microinvasion. Such a panel could increase the accuracy in the diagnosis of microinvasive lesions and as such contribute to a more objective and reproducible diagnosis, and thus treatment, of serous ovarian tumors.