Miracle-dz/UniFAR-FTUs huggingface dataset:
Importance of Bacillithiol in the Oxidative Stress Response of Staphylococcus aureus

ABSTRACT In Staphylococcus aureus, the low-molecular-weight thiol called bacillithiol (BSH), together with cognate S-transferases, is believed to be the counterpart to the glutathione system of other organisms. To explore the physiological role of BSH in S. aureus, we constructed mutants with the deletion of bshA (sa1291), which encodes the glycosyltransferase that catalyzes the first step of BSH biosynthesis, and fosB (sa2124), which encodes a BSH-S-transferase that confers fosfomycin resistance, in several S. aureus strains, including clinical isolates. Mutation of fosB or bshA caused a 16- to 60-fold reduction in fosfomycin resistance in these S. aureus strains. High-pressure liquid chromatography analysis, which quantified thiol extracts, revealed some variability in the amounts of BSH present across S. aureus strains. Deletion of fosB led to a decrease in BSH levels. The fosB and bshA mutants of strain COL and a USA300 isolate, upon further characterization, were found to be sensitive to H2O2 and exhibited decreased NADPH levels compared with those in the isogenic parents. Microarray analyses of COL and the isogenic bshA mutant revealed increased expression of genes involved in staphyloxanthin synthesis in the bshA mutant relative to that in COL under thiol stress conditions. However, the bshA mutant of COL demonstrated decreased survival compared to that of the parent in human whole-blood survival assays; likewise, the naturally BSH-deficient strain SH1000 survived less well than its BSH-producing isogenic counterpart. Thus, the survival of S. aureus under oxidative stress is facilitated by BSH, possibly via a FosB-mediated mechanism, independently of its capability to produce staphyloxanthin.
Invisible marker tracking for AR

We introduce a novel tracking system based on invisible markers which are created/drawn with an IR fluorescent pen. The tracking system consists of one scene camera, one IR camera, and one half mirror. The two cameras are positioned in each side of half mirror so that their optical centers coincide with each other. We track the invisible markers using the IR camera and visualize AR in the view of the scene camera. Thus, it works as a robust marker-less tracking system. Experimental results are given to demonstrate the viability of the proposed system.
Cognitive Map Formation Through Sequence Encoding by Theta Phase Precession

The rodent hippocampus has been thought to represent the spatial environment as a cognitive map. The associative connections in the hippocampus imply that a neural entity represents the map as a geometrical network of hippocampal cells in terms of a chart. According to recent experimental observations, the cells fire successively relative to the theta oscillation of the local field potential, called theta phase precession, when the animal is running. This observation suggests the learning of temporal sequences with asymmetric connections in the hippocampus, but it also gives rather inconsistent implications on the formation of the chart that should consist of symmetric connections for space coding. In this study, we hypothesize that the chart is generated with theta phase coding through the integration of asymmetric connections. Our computer experiments use a hippocampal network model to demonstrate that a geometrical network is formed through running experiences in a few minutes. Asymmetric connections are found to remain and distribute heterogeneously in the network. The obtained network exhibits the spatial localization of activities at each instance as the chart does and their propagation that represents behavioral motions with multidirectional properties. We conclude that theta phase precession and the Hebbian rule with a time delay can provide the neural principles for learning the cognitive map.
MR1 antigen presentation to mucosal-associated invariant T cells was highly conserved in evolution

Several nonclassical major histocompatibilty antigens (class Ib molecules) have emerged as key players in the early immune response to pathogens or stress. Class Ib molecules activate subsets of T cells that mount effector responses before the adaptive immune system, and thus are called innate T cells. MR1 is a novel class Ib molecule with properties highly suggestive of its regulation of mucosal immunity. The Mr1 gene is evolutionarily conserved, is non-Mhc linked, and controls the development of mucosal-associated invariant T (MAIT) cells. MAIT cells preferentially reside in the gut, and their development is dependent on commensal microbiota. Although these properties suggest that MAIT cells function as innate T cells in the mucosa, this has been difficult to test, due to the (i) paucity of MAIT cells that display MR1-specific activation in vitro and (ii) lack of knowledge of whether or not MR1 presents antigen. Here we show that both mouse and human MAIT cells display a high level of cross-reactivity on mammalian MR1 orthologs, but with differences consistent with limited ligand discrimination. Furthermore, acid eluates from recombinant or cellular MR1 proteins enhance MAIT cell activation in an MR1-specific and cross-species manner. Our findings demonstrate that the presentation pathway of MR1 to MAIT cells is highly evolutionarily conserved.
Locating internet routing instabilities

This paper presents a methodology for identifying the autonomous system (or systems) responsible when a routing change is observed and propagated by BGP. The origin of such a routing instability is deduced by examining and correlating BGP updates for many prefixes gathered at many observation points. Although interpreting BGP updates can be perplexing, we find that we can pinpoint the origin to either a single AS or a session between two ASes in most cases. We verify our methodology in two phases. First, we perform simulations on an AS topology derived from actual BGP updates using routing policies that are compatible with inferred peering/customer/provider relationships. In these simulations, in which network and router behavior are "ideal", we inject inter-AS link failures and demonstrate that our methodology can effectively identify most origins of instability. We then develop several heuristics to cope with the limitations of the actual BGP update propagation process and monitoring infrastructure, and apply our methodology and evaluation techniques to actual BGP updates gathered at hundreds of observation points. This approach of relying on data from BGP simulations as well as from measurements enables us to evaluate the inference quality achieved by our approach under ideal situations and how it is correlated with the actual quality and the number of observation points.
Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2.

A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way DNA junctions. The activity cuts with high specificity a synthetic four-way junction containing a 12 bp core of homologous sequences but has no activity on another four-way junction (with a fixed crossover point), a three-way junction, linear duplex DNA or duplex DNA containing six mismatched nucleotides in the centre. The major cleavage sites map as single nicks in the vicinity of the crossover point, 3' of a thymidine residue. These data indicate that the activity has a strong DNA structure selectivity as well as a limited sequence preference; features similar to the Holliday junction resolving enzymes RuvC of Escherichia coli and the mitochondrial CCE1 (cruciform-cuttingenzyme 1) of Saccharomyces cerevisiae. A putative homologue of CCE1 in S.pombe (YDC2_SCHPO) has been identified through a search of the sequence database. The open reading frame of this gene has been cloned and the encoded protein, YDC2, expressed in E.coli . The purified recombinant YDC2 exhibits Holliday junction resolvase activity and is, therefore, a functional S.pombe homologue of CCE1. The resolvase YDC2 shows the same substrate specificity and produces identical cleavage sites as the activity obtained from S. pombe cells. Both YDC2 and the cellular activity cleave Holliday junctions in both orientations to give nicks that can be ligated in vitro. The partially purified Holliday junction resolving enzyme in fission yeast is biochemically indistinguishable from recombinant YDC2 and appears to be the same protein.
Risk Factors of Delayed Surgical Intervention after Conservatively Treated Acute Traumatic Subdural Hematoma

Objective Acute subdural hematoma (ASDH) is generally considered a condition that should be managed surgically. However, some patients initially receive conservative treatment, a subset of whom require surgical intervention later. This study aimed to evaluate the predictors of delayed surgical intervention in ASDH patients who are initially managed conservatively. Methods From January 2007 to December 2015, 842 patients diagnosed with ASDH were treated at our institution. Among them, 158 patients with convexity ASDH were initially treated conservatively. Patients were divided into a delayed surgery group and a conservative group. Demographic characteristics, past medication and medical histories, and radiological and laboratory data were collected by retrospective chart review. Independent risk factors were identified with univariate and multivariate analyses. Results Twenty-eight patients (17.7%) underwent delayed surgical intervention. Their mean age was 69.0 years, and 82.1% were male. Hypertension, diabetes mellitus, and heart disease prevalence and use of anti-platelet agents did not significantly differ from the conservative group. However, age (p=0.024), previous cerebral infarction history (p=0.026), increased maximal hematoma thickness (p<0.001), midline shifting (p=0.001) and accompanying subarachnoid hemorrhage (p=0.022) on initial brain computed tomography (CT) scan, low hemoglobin level (p<0.001), high leukocyte count (p=0.004), and low glucose level (p=0.002) were significantly associated with delayed surgical intervention. In multivariate analysis, increased maximal hematoma thickness (odds ratio [OR]=1.279, 95% confidence interval [CI] 1.075–1.521; p=0.006), low hemoglobin level (OR=0.673, 95% CI 0.467–0.970; p=0.034), and high leukocyte count (OR=1.142, 95% CI 1.024–1.272; p=0.017) were independent risk factors for delayed surgical intervention. Conclusion Due to the high likelihood of delayed surgical intervention among minimal ASDH patients with a thicker hematoma on initial brain CT, lower hemoglobin level, and higher leukocyte count, these patients should receive more careful observation.
CYTOKINE EXPRESSION AND SPECIFIC LYMPHOCYTE PROLIFERATION IN TWO STRAINS OF CRYPTOSPORIDIUM PARVUM-INFECTED γ-INTERFERON KNOCKOUT MICE

Differences in the immune response between 2 strains of interferon-γ knockout mice (BALB/c-GKO and C57BL/6-GKO) infected with Cryptosporidium parvum were examined because the course of infection among these 2 strains is markedly different. Infection of the BALB/c-GKO with C. parvum (2 × 106 oocysts/mouse) resulted in slight weight loss, oocyst shedding, and recovery from infection by 2 wk postinfection (PI). Infection with 100 oocysts in the C57BL/6-GKO mice resulted in significant weight loss, oocyst shedding, and death by day 10 PI. Splenocytes from infected mice were able to proliferate in a dose-dependent manner to soluble C. parvum-sporozoite antigen (SAg). In vitro stimulation with SAg resulted in an increase in interleukin (IL)-2, IL-4, IL-5, and tumor necrosis factor-α mRNA cytokine expression from splenocytes of infected BALB/c-GKO mice. In contrast, only IL-5 mRNA expression was increased in the splenocytes from C. parvum-infected C57BL/6-GKO mice. Phenotypic analysis indicated no significant differences in the splenic cell populations. Previous studies indicated that susceptibility to C. parvum is dependent on CD4+ T cells and interferon-γ production. The present study indicates that although both of these strains of knockout mice become infected with C. parvum, resolution of infection may be in part dependent on the expression of Th2 cytokines.
Postech’s System Description for Medical Text Translation Task

This short paper presents a system description for intrinsic evaluation of the WMT 14’s medical text translation task. Our systems consist of phrase-based statistical machine translation system and query translation system between German-English language pairs. Our work focuses on the query translation task and we achieved the highest BLEU score among the all submitted systems for the English-German intrinsic query translation evaluation.
Effects of Acute Aerobic Exercise on Executive Function in Older Women

In the older adult population, there is a high rate of decline in cognitive functioning. It has been reported that improving fitness levels exhibits a positive effect on cognition (Colcombe & Kramer, 2003; Colcombe et al., 2004). Older adult females 60-75 years of age who were moderately to highly fit performed better on working memory tasks after an acute bout of moderate or high-intensity aerobic exercise than before exercise. Participants included healthy females (N = 11) who were not on medications for cognitive impairment and who did not show signs of dementia or Alzheimer's disease. A submaximal walking exercise test was administered to assess fitness levels, as well as modified flanker tasks and d2-tests, to measure executive functioning in the brain. Results indicated that older adults females, 60-75 years of age showed improved scores on the modified flanker task and d2-tests after a session of moderate or high-intensity aerobic exercise. These findings suggest that an acute bout of exercise, regardless of intensity, can improve tests of executive control.
Stress and the Mobility Training Process: A Literature Review

Stress is considered to be a major problem in the mobility training process. The effects of stress, potential stress reduction techniques, factors which impact independent mobility, and suggested areas of future research are discussed in this literature review. Practitioners should consider implementing some of these strategies to alleviate the anxiety of their visually impaired students and clients during mobility training. Hopefully, a greater number of professionals will become more involved in conducting formalized research projects in this area.
Population dynamics of Antarctophthirus microchir (Anoplura: Echinophthiriidae) in pups from South American sea lion, Otaria flavescens, in Northern Patagonia

SUMMARY We analysed population dynamics of the louse Antarctophthirus microchir in pups of the South American sea lion, Otaria flavescens, at the Punta León rookery (Argentina) over a period of 2 years. A total of 136 pups were aged and marked at the beginning of the lactation period ashore, then sampled for lice at different times within 30 days. Sampling was restricted to the chest and belly, two sites where lice were especially abundant. This concentration on ventral areas might protect lice from thermal stress in the austral summer. Infestation patterns in pups ⩽3 days old suggested that the potential for transmission increased from first nymphs to adults. Population trends of each instar with pup age, based on standardised values of abundance, were conserved between years, reflecting the basic dynamics of recruitment and reproduction. However, trends based on log-transformed abundances varied significantly between years; apparently, environmental conditions affected growth of lice populations differently each year. Stage-based deterministic models for population growth of A. microchir suggested generation times from 18 to 23 days. Accordingly, only 2 lice generations might be produced before pups start going to the sea. Shortening the cycle to accommodate a third generation might be risky, whereas a 2-generation cycle might at least result in larger females producing higher numbers of viable offspring.
Characteristics Associated With Maintenance of Mean A1C <6.5% in People With Dysglycemia in the ORIGIN Trial

OBJECTIVE To assess the success and baseline predictors of maintaining glycemic control for up to 5 years of therapy using basal insulin glargine or standard glycemic care in people with dysglycemia treated with zero or one oral glucose-lowering agents. RESEARCH DESIGN AND METHODS Data from 12,537 participants in the Outcome Reduction with Initial Glargine Intervention (ORIGIN) trial were examined by baseline glycemic status (with or without type 2 diabetes) and by therapeutic approach (titrated insulin glargine or standard therapy) using an intention-to-treat analysis. Median values for fasting plasma glucose (FPG) and A1C and percentages with A1C <6.5% (48 mmol/mol) during randomized treatment were calculated. Factors independently associated with maintaining updated mean A1C <6.5% were analyzed with linear regression models. RESULTS Median A1C in the whole population was 6.4% at baseline; at 5 years, it was 6.2% with glargine treatment and 6.5% with standard care. Of those with diabetes at baseline, 60% using glargine and 45% using standard care had A1C <6.5% at 5 years. Lack of diabetes and lower baseline A1C were independently associated with 5-year mean A1C <6.5%. Maintaining mean A1C <6.5% was more likely with glargine (odds ratio [OR] 2.98 [95% CI 2.67–3.32], P < 0.001) than standard care after adjustment for other independent predictors. CONCLUSIONS Systematic intervention with basal insulin glargine or standard care early in the natural history of dysglycemia can maintain glycemic control near baseline levels for at least 5 years, whether diabetes is present at baseline or not. Keeping mean A1C <6.5% is more likely in people with lower baseline A1C and with the glargine-based regimen.
MoNbTaV Medium-Entropy Alloy

Guided by CALPHAD (Calculation of Phase Diagrams) modeling, the refractory medium-entropy alloy MoNbTaV was synthesized by vacuum arc melting under a high-purity argon atmosphere. A body-centered cubic solid solution phase was experimentally confirmed in the as-cast ingot using X-ray diffraction and scanning electron microscopy. The measured lattice parameter of the alloy (3.208 A) obeys the rule of mixtures (ROM), but the Vickers microhardness (4.95 GPa) and the yield strength (1.5 GPa) are about 4.5 and 4.6 times those estimated from the ROM, respectively. Using a simple model on solid solution strengthening predicts a yield strength of approximately 1.5 GPa. Thermodynamic analysis shows that the total entropy of the alloy is more than three times the configurational entropy at room temperature, and the entropy of mixing exhibits a small negative departure from ideal mixing.
Social Media and the Science of Health Behavior

Social influences are a primary factor in the adoption of health behaviors.1,2 Compliance with diet and nutrition programs, adherence to preventive screening recommendations, and maintenance of exercise routines all can depend on having contact with friends and family who also engage in these behaviors. In addition to a great deal of literature on peer effects,3 recent studies of large network data sets have made important advances in our understanding of how social networks influence the collective dynamics of health behavior.4,5 Research has shown that social influences can affect collective health outcomes ranging from epidemic obesity to smoking behaviors, which have important consequences both for theoretical models of social epidemiology and for the practical design of interventions and treatment strategies.6,7 These findings have direct implications for research aimed at understanding how social influences on dieting, exercising, medication use, and getting screenings can impact behavior change affecting cardiovascular disease. The large number of health domains affected by recent research on the spread of behaviors has made social diffusion a topic of growing interest for an increasing variety of researchers and practitioners who are concerned with understanding the social dimensions of health. This article discusses the development of new methods that use social media to study these health dynamics. Although there is widespread theoretical and practical interest in understanding how social influences affect health-related behaviors, empirical studies of the social dynamics of health face important methodological challenges. Large observational studies of population health have faced the limitation that they are unable to address problems of causal identification.8,9 Extant studies have been able to show conclusively that health-related traits such as smoking5 and weight gain4 correlate with social ties in a network, yet the data do not provide a …
The Issue of Generalization in Q Methodology: "Reliable Schematics" Revisited

Research employing Q technique and its attendant methodology has long encountered criticism targeted on the allegedly specious, "non-generalizable" nature of such findings. Drawn typically from small-sample investigations of human subjectivity, wherein respondents supply data through Q sorts composed of items of unknown reliability, findings of Q studies are considered by many to fall far short of the minimal criteria for scientific measurement. Issues of generalization in Q methodology, it is argued, are amenable to examination in terms of the notion of "reliable schematics. " Findings from two pairs of "tandem-study" explorations bear strong witness to the schematically reliable character of Q-study results produced from differing probes of the same subjective phenomenon. Viewed against this backdrop, the frequently voiced concerns over reliability issues stemming from the use of Q would appear quite exaggerated if not altogether unfounded.
Intergenerational Closure and Academic Achievement in High School: A New Evaluation of Coleman's Conjecture

This article reexamines the conjecture of James S. Coleman that intergenerational social closure promotes student achievement in high schools, analyzing the best national data on academic achievement and social networks: the 2002 and 2004 waves of the Education Longitudinal Study. The results show that within the Catholic school sector, schools that are characterized by dense parental networks have substantially higher average student achievement. This association can be reduced but not eliminated by conditioning on available measures of student network structure and standard measures of family background. In contrast, in the public school sector, a similarly strong bivariate association between dense parental networks and student achievement can be attributed almost entirely to these basic conditioning variables. These results represent, at best, a mixed verdict for Coleman's predictions. Intergenerational closure in its currently observed form does not increase achievement in public schools, suggesting that parental monitoring of discipline does not outweigh some of the costs of parental closure. However, intergenerational closure may increase achievement in Catholic schools to a modest degree because Catholic schools are affiliated with religious communities that have appropriable norms.