Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect and Mechanism of Thrombospondin-1 on the Angiogenesis Potential in Human Endothelial Progenitor Cells: An In Vitro Study

Objective Coronary collateral circulation plays a protective role in patients with coronary artery disease (CAD). We investigated whether thrombospondin-1(TSP-1) has an inhibitory effect on angiogenesis potential in endothelial progenitor cells(EPCs) and tested whether TSP-1 are altered in plasma of patients who had chronic total occlusion (CTO) in at least one coronary artery and with different collateral stages(according to Rentrop grading system). Methods and Results We isolated early and late EPCs from human cord blood and investigated a dose-dependent effect of TSP-1 on their angiogenesis potential by Matrigel angiogenesis assay. We found that TSP-1 (5 µg/ml) inhibited early EPCs incorporation into tubules after pretreatment for 1, 6 and 12 hours, respectively (83.3±11.9 versus 50.0±10.1 per field for 1 hour,161.7±12.6 versus 124.0±14.4 for 6 hours, 118.3±12.6 versus 68.0±20.1 for 12 hours, p<0.05). TSP-1 also inhibited late EPCs tubule formation at 1 µg/ml (6653.4±422.0 µm/HPFversus 5552.8±136.0 µm/HPF, p<0.05), and the inhibition was further enhanced at 5 µg/ml (6653.4±422.0 µm/HPF versus 2118.6±915.0 µm/HPF p<0.01). To explore the mechanism involved, a small interfering RNA was used. In vitro, CD47 siRNA significantly attenuated TSP-1's inhibition of angiogenesis on late EPCs and similar results were obtained after functional blocking by anti-CD47 antibody. Then we investigated pathways downstream of CD47 and found TSP-1 regulated VEGF-induced VEGFR2 phosphorylation via CD47. Furthermore, we examined plasma TSP-1 levels in patients with CTO who developed different stages of collaterals and found a paradoxical higher level of TSP-1 in patients with good collaterals compared with bad ones (612.9±554.0 ng/ml versus 224.4±132.4 ng/ml, p<0.05). Conclusion TSP-1 inhibited angiogenesis potential of early and late EPCs in vitro. This inhibition may be regulated by TSP-1's interaction with CD47, resulting in down regulation of VEGFR2 phosphorylation. In patients with CTO, there may be a self-adjustment mechanism in bad collaterals which is shown as low level of angiogenesis inhibitor TSP-1, and thus favoring collateral formation.
ACTIONS OF SOME AUTACOIDS AND PEPTIDES, INCLUDING RELAXIN, ON COSTO‐UTERINE MUSCLE FROM RATS

1. The actions of angiotensin II, bradykinin, oxytocin, arginine vasopressin, relaxin, serotonin and the prostaglandins E2 and F2α were examined on preparations of costo‐uterine muscle from stilboestrol‐treated rats.
Assessing Optimal Illumination for Visual Response Accuracy in Visually Impaired Adults

The currently accepted position concerning recommended lighting for visually impaired individuals is that individually identified and specified lighting is required for these persons to attain optimal visual performance. However, there is presently no formula for predicting the placement or intensity of a light source needed for any given patient, nor are there any specific rules for determining optimal illumination for visually impaired individuals. The purpose of the present study was to determine (a) if individual levels of optimal illumination could be reliably determined for visually impaired adults, and (b) if the level identified in assessment had validity in relation to a practical reading task. Visual response accuracy was assessed across 10 levels of illumination which were all greater than normal room lighting. Each level was presented 4 times for a total of 40 experimental trials.
A Biomechanical Analysis by Skill Level of Free Throw Shooting in Basketball

Free throw shooting in basketball is a task which falls into two broad categories of sports skills. First. it is a task of accuracy_ Second. it requires submaximal velocity for most populations of players. Because of the submaximal velocity demands. there are endless combinations of segmental contribu­ tions in conjunction with numerous projection angles and velo­ cities which can result in shots which directly or indirectly fall through the basket. In addition to the segmental actions which contribute to shooting performance. there may be other actions which are extraneous to performance. These non-related characteristics can be termed style. Since the identification of characteristics which are consistently employed by skilled per­ formers and conspicuously absent in poor performers may lead to improved teaching and coaching. this study was conducted to ana­ lyze selected biomechanical parameters of free throw shooting by players of varying skill.
A new method for the diagnosis of systemic amyloidosis.

Twenty-eight patients were clinically suspected of secondary amyloidosis; eight of them had abnormal results from fine-needle biopsy of subcutaneous abdominal fat. The abnormal results were later confirmed after rectal biopsy or autopsy. In addition, seven of the 19 patients with normal results also had normal results from rectal biopsies. Fine-needle biopsy of subcutaneous fat is a simple and reliable method for diagnosing secondary systemic amyloidosis.
The ribosomal RNA gene cluster in aneuploid chickens: evidence for increased gene dosage and regulation of gene expression

In the chicken, the nucleolus organizer regions, or sites of the genes encoding 18S, 5.8S, and 28S ribosomal RNA (rRNA), map to one pair of microchromosomes that can be identified by silver nitrate cytochemistry. This nucleolar organizer chromosome also contains the major histocompatibility complex. Chickens aneuploid for this chromosome have been identified and reproduced for over seven generations. Crossing two trisomic parents results in the production of viable disomic, trisomic, and tetrasomic progeny, showing two, three, and four nucleoli and nucleolar organizers per cell, respectively. A molecular analysis of rRNA genes was undertaken to establish the gene copy numbers in the aneuploid genotypes, and to determine if elevated numbers of rRNA genes are stably maintained and inherited over multiple generations. Gene copy numbers were determined using hybridization analysis of erythrocyte DNA obtained from individuals comprising a family which segregated disomic, trisomic, and tetrasomic genotypes. The values obtained were 290, 420, and 570 rDNA repeats per cell for disomic, trisomic, and tetrasomic animals, respectively. These results provide molecular confirmation of the two aneuploid states and show that elevated gene copy numbers have been maintained over multiple generations. Fibroblasts derived from disomic and tetrasomic embryos were found to grow at similar rates in culture, and mature rRNA levels in chicken embryo fibroblasts from disomic, trisomic and tetrasomic embryos were also found to have similar levels of mature rRNA. Therefore, despite the increase in rDNA content, the level of rRNA is regulated to diploid amounts in aneuploid fibroblasts.
Towards a Definition of Knowledge Graphs

Recently, the term knowledge graph has been used frequently in research and business, usually in close association with Semantic Web technologies, linked data, large-scale data analytics and cloud computing. Its popularity is clearly in-ﬂuenced by the introduction of Google’s Knowledge Graph in 2012, and since then the term has been widely used without a deﬁnition. A large variety of interpretations has hampered the evolution of a common understanding of knowledge graphs. Numerous research papers refer to Google’s Knowledge Graph, although no oﬃcial documentation about the used methods exists. The prerequisite for widespread academic and commercial adoption of a concept or technology is a common understanding, based ideally on a deﬁnition that is free from ambiguity. We tackle this issue by discussing and deﬁning the term knowledge graph, considering its history and diversity in interpretations and use. Our goal is to propose a deﬁnition of knowledge graphs that serves as basis for discussions on this topic and contributes to a common vision.
Concentrations of serum amyloid A and plasma fibrinogen in horses undergoing emergency abdominal surgery.

OBJECTIVE To compare the perioperative response of serum amyloid A (SAA) to fibrinogen in horses requiring exploratory celiotomy for colic and to determine if SAA could be used to predict complications and outcome. DESIGN Prospective observational clinical study. SETTING University teaching hospital. ANIMALS Eighteen horses undergoing exploratory celiotomy for colic. Inclusion criteria for the study included survival and anesthetic recovery from exploratory celiotomy, no history of surgery within the past year. INTERVENTIONS Blood was obtained via jugular venipuncture before surgery (time 0) and at 24, 48, 72, and 96 hours after recovery from anesthesia. MEASUREMENTS AND MAIN RESULTS Quantitative and semiquantitative fibrinogen, SAA, total nucleated cell counts, and total protein were evaluated at each time point. Multivariable linear regression was used to assess differences at each time point and after grouping horses according to duration of colic prior to surgery, strangulating surgical lesion or not, presence of systemic inflammatory response syndrome (SIRS) on admission, and postsurgical complications. Significant (P < 0.05) increases in SAA concentrations occurred in all cases after surgery compared to fibrinogen concentration, which only demonstrated a mild, clinically insignificant increase postsurgery. SAA concentrations were also significantly increased (P < 0.05) in cases identified with SIRS prior to surgery and postoperatively at 48 (P = 0.05) and 72 hours (P = 0.02) in horses that developed complications. CONCLUSIONS Measurement of SAA is a more sensitive indicator of inflammation than fibrinogen in the perioperative period of horses requiring exploratory celiotomy for colic. Serial measurement of SAA at 48, 72, and 96 hours after surgery may be helpful to determine risk of complications and guide postoperative management. Measurement of SAA on admission also allows for quantification of SIRS when it is detected clinically.
Where is the effect of frequency in word production? Insights from aphasic picture-naming errors

Some theories of lexical access in production locate the effect of lexical frequency at the retrieval of a word's phonological characteristics, as opposed to the prior retrieval of a holistic representation of the word from its meaning. Yet there is evidence from both normal and aphasic individuals that frequency may influence both of these retrieval processes. This inconsistency is especially relevant in light of recent attempts to determine the representation of another lexical property—age of acquisition or AoA—whose effect is similar to that of frequency. To further explore the representations of these lexical variables in the word retrieval system, we performed hierarchical, multinomial logistic regression analyses of 50 aphasic patients’ picture-naming responses. While both log frequency and AoA had a significant influence on patient accuracy and led to fewer phonologically related errors and omissions, only log frequency had an effect on semantically related errors. These results provide evidence for a lexical access process sensitive to frequency at all stages, but with AoA having a more limited effect.
Women’s Land Claims in the Acholi Region of Northern Uganda: What Can Be Learned from What Is Contested

Women are often understood to be highly marginalised in typical African customary land regimes. The research presented in this article found that in the Acholi region of northern Uganda this is not the case. The crisis of land conflict that followed the twenty-year lra insurgency and mass rural displacement has seemingly passed, notwithstanding a minimal contribution from the formal justice, law and order sector: local state actors as well as clan elders are mediating and adjudicating disputes on the basis of custom. However some social institutions, in particular traditional marriage, have been deeply affected by displacement and the consequent poverty. In this context, custom appears to be becoming more responsive to the needs of women, including those who are divorced or separated. While women’s customary land claims are often challenged, they appear to be generally respected and supported by communities and those with responsibilities for settling disputes.
Genomic Selection for Drought Tolerance Using Genome-Wide SNPs in Maize

Traditional breeding strategies for selecting superior genotypes depending on phenotypic traits have proven to be of limited success, as this direct selection is hindered by low heritability, genetic interactions such as epistasis, environmental-genotype interactions, and polygenic effects. With the advent of new genomic tools, breeders have paved a way for selecting superior breeds. Genomic selection (GS) has emerged as one of the most important approaches for predicting genotype performance. Here, we tested the breeding values of 240 maize subtropical lines phenotyped for drought at different environments using 29,619 cured SNPs. Prediction accuracies of seven genomic selection models (ridge regression, LASSO, elastic net, random forest, reproducing kernel Hilbert space, Bayes A and Bayes B) were tested for their agronomic traits. Though prediction accuracies of Bayes B, Bayes A and RKHS were comparable, Bayes B outperformed the other models by predicting highest Pearson correlation coefficient in all three environments. From Bayes B, a set of the top 1053 significant SNPs with higher marker effects was selected across all datasets to validate the genes and QTLs. Out of these 1053 SNPs, 77 SNPs associated with 10 drought-responsive transcription factors. These transcription factors were associated with different physiological and molecular functions (stomatal closure, root development, hormonal signaling and photosynthesis). Of several models, Bayes B has been shown to have the highest level of prediction accuracy for our data sets. Our experiments also highlighted several SNPs based on their performance and relative importance to drought tolerance. The result of our experiments is important for the selection of superior genotypes and candidate genes for breeding drought-tolerant maize hybrids.
The (N)Ever-Changing World: Stability and Change in Organizational Routines

This paper uses data on invoice processing in four organizations to distinguish empirically between two competing theories of organizational routines. One theory predicts that routines should generate patterns of action that are few in number and stable over time, and that atypical patterns of action are driven primarily by exceptional inputs. The competing theory predicts the opposite. By modeling the routines as networks of action and using a first-order Markov model to test for stationarity, we find support for the competing theory. The routines generated hundreds of unique patterns that changed significantly during a five-month period without any apparent external intervention. Changes did not appear to reflect improved performance or learning. Furthermore, we found that exogenous factors (such as large invoices from unusual vendors) are not associated with atypical patterns of action, but endogenous factors (such as the experience of the participants) are. We also found that increased automation can increase variation under some circumstances. These findings offer empirical support for endogenous change in organizational routines and underscore the importance of the sociomaterial context in understanding stability and change.
DR-nm23 gene expression in neuroblastoma cells: relationship to integrin expression, adhesion characteristics, and differentiation.

BACKGROUND Neuroblastoma, a childhood tumor originating from cells of the embryonic neural crest, retains the ability to differentiate, yielding cells with epithelial-Schwann-like, neuronal, or melanocytic characteristics. Since nm23 gene family members have been proposed to play a role in cellular differentiation, as well as in metastasis suppression, we investigated whether and how DR-nm23, a recently identified third member of the human nm23 gene family, might be involved in neuroblastoma differentiation. METHODS Three neuroblastoma cell lines (human LAN-5, human SK-N-SH, and murine N1E-115) were used in these experiments; cells from two of the lines (SK-N-SH and N1E-115) were also studied after being stably transfected with a plasmid containing a full-length DR-nm23 complementary DNA. Cellular expression of specific messenger RNAs and proteins was assessed by use of standard techniques. Cellular adhesion to a variety of protein substrates was also evaluated. RESULTS DR-nm23 messenger RNA levels in nontransfected LAN-5 and SK-N-SH cells generally increased with time after exposure to differentiation-inducing conditions; levels of the other two human nm23 messenger RNAs (nm23-H1 and nm23-H2) remained essentially constant. Transfected SK-N-SH cells overexpressing DR-nm23 exhibited some characteristics of differentiated cells (increased vimentin and collagen type IV expression) even in the absence of differentiation-inducing conditions. Compared with control cells, DR-nm23-transfected cells exposed to differentiation-inducing conditions showed a greater degree of growth arrest (SK-N-SH cells) and greater increases in integrin protein expression, especially of integrin beta1 (N1E-115 cells). DR-nm23-transfected N1E-115 cells also showed a marked increase in adhesion to collagen type I-coated tissue culture plates that was inhibited by preincubation with an anti-integrin beta1 antibody. CONCLUSIONS DR-nm23 gene expression appears to be associated with differentiation in neuroblastoma cells and may affect cellular adhesion through regulation of integrin protein expression.
A fast MAC layer handoff protocol for WiFi-based wireless networks

In next generation mobile heterogeneous networks, WiFi-based wireless networks are becoming important components, because WiFi devices are widely used in laptops, PDAs and other mobile computing machines. In a WiFi-based wireless network, handoff management is a key service, as the radio range of the WiFi device is limited. Moreover, providing seamless roaming in wireless networks is mandatory for supporting realtime applications in a mobile environment, such as VoIP, online games, and eConference. Recently, many solutions have been introduced to reduce MAC layer handoff latency; however, these solutions consider every mobile node separately, which would not be suitable for a large-scale environment. This paper proposes a novel MAC layer handoff protocol for large-scale WiFi-based wireless networks to support seamless real-time applications. In our scheme, before the mobile node starts initiating the MAC layer handoff process, it selects several neighboring nodes to help it scan available channels. All channels are divided into groups and scanned by these neighbors separately. Therefore, the number of scanning channels in each node is reduced, and scanning latency is minimized. According to simulation results in ns2, we conclude that our scheme can shorten MAC layer handoff latency greatly, and also achieve seamless handoff in terms of loss ratio of data packets.
Suppression of a cold-sensitive mutation in ribosomal protein S5 reveals a role for RimJ in ribosome biogenesis

A specific mutation of Escherichia coli ribosomal protein S5, in which glycine is changed to aspartate at position 28 [S5(G28D)], results in cold sensitivity and defects in ribosome biogenesis and translational fidelity. In an attempt to understand the roles of S5 in these essential cellular functions, we selected extragenic suppressors and identified rimJ as a high‐copy suppressor of the cold‐sensitive phenotype associated with the S5(G28D) mutation. Our studies indicate that RimJ overexpression suppresses the growth defects, anomalous ribosome profiles and mRNA misreading exhibited by the S5(G28D) mutant strain. Although previously characterized as the N‐acetyltransferase of S5, our data indicate that RimJ, when devoid of acetyltransferase activity, can suppress S5(G28D) defects thus indicating that the suppression activity of RimJ is not dependent on its acetyltransferase activity. Additionally, RimJ appears to associate with pre‐30S subunits indicating that it acts on the ribonucleoprotein particle. These findings suggest that RimJ has evolved dual functionality; it functions in r‐protein acetylation and as a ribosome assembly factor in E. coli.
Endothelial integrin α3β1 stabilizes carbohydrate-mediated tumor/endothelial cell adhesion and induces macromolecular signaling complex formation at the endothelial cell membrane

Blood borne metastatic tumor cell adhesion to endothelial cells constitutes a critical rate-limiting step in hematogenous cancer metastasis. Interactions between cancer associated carbohydrate Thomsen-Friedenreich antigen (TF-Ag) and endothelium-expressed galectin-3 (Gal-3) have been identified as the leading molecular mechanism initiating tumor/endothelial cell adhesion in several types of cancer. However, it is unknown how these rather weak and transient carbohydrate/lectin mediated interactions are stabilized. Here, using Western blot and LC tandem mass spectrometry analyses of pull-downs utilizing TF-Ag loaded gold nanoparticles, we identified Gal-3, endothelial integrin α3β1, Src kinase, as well as 5 additional molecules mapping onto focal adhesion pathway as parts of the macromolecular complexes formed at the endothelial cell membranes downstream of TF-Ag/Gal-3 interactions. In a modified parallel flow chamber assay, inhibiting α3β1 integrin greatly reduced the strength of tumor/endothelial cell interactions without affecting the initial cancer cell adhesion. Further, the macromolecular complex induced by TF-Ag/Gal-3/α3β1 interactions activates Src kinase, p38, and ERK1/2, pathways in endothelial cells in a time- and α3β1-dependent manner. We conclude that, following the initial metastatic cell attachment to endothelial cells mediated by TF-Ag/Gal-3 interactions, endothelial integrin α3β1 stabilizes tumor/endothelial cell adhesion and induces the formation of macromolecular signaling complex activating several major signaling pathways in endothelial cells.
Application of a null-beamformer to source localisation in MEG data of deep brain stimulation

In this paper, we present an analysis of magnetoencephalography (MEG) signals from a patient with whole-body chronic pain in order to investigate changes in neural activity induced by DBS. The patient is one of the few cases treated using DBS of the anterior cingulate cortex (ACC). Using MEG to reconstruct the neural activity of interest is challenging because of interference to the signal from the DBS device. We demonstrate that a null-beamformer can be used to localise neural activity despite artefacts caused by the presence of DBS electrodes and stimulus pulses. We subsequently verified the accuracy of our source localisation by correlating the predicted DBS electrode positions with their actual positions, previously identified using anatomical imaging. We also demonstrated increased activity in pain-related regions including the pre-supplementary motor area, brainstem periaqueductal gray and medial prefrontal areas when the patient was in pain compared to when the patient experienced pain relief.