Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of stearoyl-coenzyme A desaturase in regulating lipid metabolism

Purpose of review Stearoyl-coenzyme A desaturase 1 is a δ-9 fatty acid desaturase that catalyzes the synthesis of monounsaturated fatty acids and has emerged as a key regulator of metabolism. This review evaluates the latest advances in our understanding of the pivotal role of stearoyl-coenzyme A desaturase 1 in health and disease. Recent findings Scd1-deficient mice have reduced lipid synthesis and enhanced lipid oxidation, thermogenesis and insulin sensitivity in various tissues including liver, muscle and adipose tissue due to transcriptional and posttranscriptional effects. These metabolic changes protect Scd1-deficient mice from a variety of dietary, pharmacological and genetic conditions that promote obesity, insulin resistance and hepatic steatosis. Stearoyl-coenzyme A desaturase 1 is required to guard against dietary unsaturated fat deficiency, leptin deficiency-induced diabetes, and palmitate-induced lipotoxic insults in muscle and pancreatic β-cells. Paradoxical observations of increased muscle stearoyl-coenzyme A desaturase 1 during obesity, starvation and exercise raise questions as to the role of stearoyl-coenzyme A desaturase 1 in this tissue. Mice with a liver-specific loss of stearoyl-coenzyme A desaturase 1, and inhibition of stearoyl-coenzyme A desaturase 1 via antisense or RNA interference, recapitulate only a subset of the phenotypes observed in global Scd1 deficiency, indicating the involvement of multiple tissues. Summary Recent studies in humans and animal models have highlighted that modulation of stearoyl-coenzyme A desaturase 1 activity by dietary intervention or genetic manipulation strongly influences several facets of energy metabolism to affect susceptibility to obesity, insulin resistance, diabetes and hyperlipidemia.
Using the Image Analysis Method for Describing Soil Detachment by a Single Water Drop Impact

The aim of the present work was to develop a method based on image analysis for describing soil detachment caused by the impact of a single water drop. The method consisted of recording tracks made by splashed particles on blotting paper under an optical microscope. The analysis facilitated division of the recorded particle tracks on the paper into drops, “comets” and single particles. Additionally, the following relationships were determined: (i) the distances of splash; (ii) the surface areas of splash tracks into relation to distance; (iii) the surface areas of the solid phase transported over a given distance; and (iv) the ratio of the solid phase to the splash track area in relation to distance. Furthermore, the proposed method allowed estimation of the weight of soil transported by a single water drop splash in relation to the distance of the water drop impact. It was concluded that the method of image analysis of splashed particles facilitated analysing the results at very low water drop energy and generated by single water drops.
Outcome of Adult Horses with Botulism Treated at a Veterinary Hospital: 92 Cases (1989–2013)

Background There are no studies evaluating a large population of adult horses treated for botulism. Reported survival rates in outbreak situations are low; however, many horses in outbreaks do not receive treatment. Hypothesis/Objectives That adult horses treated at a veterinary hospital would have improved survival compared to outbreak situations. Additional aims included identification of predictors of nonsurvival. Animals All horses greater than 6 months of age with a final diagnosis of botulism admitted to a veterinary teaching hospital between 1989 and 2013 were included. Methods Retrospective study. Historical, admission, and hospitalization data were retrieved from medical records and associations between variables and nonsurvival were identified using logistic regression. Two multivariable models were developed pertaining to (1) information available at admission and (2) clinical findings during hospitalization. Results Ninety‐two records met inclusion criteria. Retained variables for the two models indicated that higher rectal temperature (OR, 1.94; CI, 1.19–3.17) and dysphagia (OR, 4.04; CI, 1.01–16.17) observed at admission increased the odds of survival, as did treatment with antitoxin (OR, 121.30; CI, 9.94–1,480.65). Horses with abnormal respiratory effort or inability to stand had decreased odds of survival. Overall survival was 48% but was significantly higher (67%, P = .011) for horses that arrived standing, and even higher (95%, P < .001) for horses that remained able to stand throughout hospitalization. Complications occurred in 62% of horses but were not associated with nonsurvival. Conclusions and Clinical Importance Horses that lose the ability to stand have a poor chance of survival. Complications are common in treated horses but do not reduce survival.
The Millennium Galaxy Catalogue: the photometric accuracy, completeness and contamination of the 2dFGRS and SDSS-EDR/DR1 data sets

The Millennium Galaxy Catalogue (MGC) is a deep (μ B,lim = 26 mag arcsec -2 ), wide-field, charge-coupled device imaging survey, covering 37.5 deg 2 . The MGC survey region is completely contained within the Two-Degree Field Galaxy Redshift Survey (2dFGRS) and the Sloan Digital Sky Survey Early Data Release (SDSS-EDR). We compare the photometry and completeness of the 2dFGRS and the SDSS-EDR with the MGC over the range 16 < B < 20 mag. We have also undertaken a photometric comparison to SuperCosmos (SCOS) and the Sloan Digital Sky Survey First Data Release (SDSS-DR1). We find that B MGC - B 2dF = (0.035 ± 0.005) mag with an uncertainty of 0.142 mag per galaxy; B MGC - B SCOS = (0.032 ± 0.005) mag with an uncertainty of 0.108 mag; B MGC -B SDSS-EDR = (0.032 ± 0.005) mag with an uncertainty of 0.094 mag; and B MGC - B SDSS-DR1 = (0.039 ± 0.005) mag with an uncertainty of 0.086 mag. We find that high surface brightness 2dFGRS galaxies are systematically too faint, which leads to a significant scale error in magnitude. This effect is significantly reduced with the SCOS photometry. In the SDSS there is a weak non-linear scale error, which is negligible for faint galaxies. Low surface brightness galaxies in the SDSS are systematically fainter, consistent with the relative shallowness of this survey. We find that the 2dFGRS catalogue has (5.2 ± 0.3) per cent stellar contamination, (7.0 ± 0.4) per cent of objects resolved into two or more by the MGC, and is (8.7 ± 0.6) per cent incomplete compared to the MGC. From our all-object spectroscopic survey we find that the MGC is itself misclassifying (5.6 ± 1.3) per cent of galaxies as stars, and hence the 2dFGRS misses (14.3 ± 1.4) per cent of the galaxy population. The SDSS-EDR galaxy catalogue has (1.3 ± 0.1) per cent stellar contamination and (5.3 ± 1.0) per cent of galaxies misclassified as stars, with (0.18 ± 0.04) per cent of objects resolved into two or more by the MGC, and is (1.8 ± 0.1) per cent incomplete compared to the MGC. The total fraction of galaxies missing from the SDSS-EDR galaxy catalogue to B MGC = 20 mag, from incompleteness and misclassification, is (7.1 ± 1.0) per cent.
Nmap: A Novel Neighborhood Preservation Space-filling Algorithm

Space-filling techniques seek to use as much as possible the visual space to represent a dataset, splitting it into regions that represent the data elements. Amongst those techniques, Treemaps have received wide attention due to its simplicity, reduced visual complexity, and compact use of the available space. Several different Treemap algorithms have been proposed, however the core idea is the same, to divide the visual space into rectangles with areas proportional to some data attribute or weight. Although pleasant layouts can be effectively produced by the existing techniques, most of them do not take into account relationships that might exist between different data elements when partitioning the visual space. This violates the distance-similarity metaphor, that is, close rectangles do not necessarily represent similar data elements. In this paper, we propose a novel approach, called Neighborhood Treemap (Nmap), that seeks to solve this limitation by employing a slice and scale strategy where the visual space is successively bisected on the horizontal or vertical directions and the bisections are scaled until one rectangle is defined per data element. Compared to the current techniques with the same similarity preservation goal, our approach presents the best results while being two to three orders of magnitude faster. The usefulness of Nmap is shown by two applications involving the organization of document collections and the construction of cartograms illustrating its effectiveness on different scenarios.
Review and current status of emulsion/dispersion technology using an internal gelation process for the design of alginate particles

Emulsification/internal gelation has been suggested as an alternative to extrusion/external gelation in the encapsulation of several compounds including sensitive biologicals such as protein drugs. Protein-loaded microparticles offer an inert environment within the matrix and encapsulation is conducted at room temperature in a media free of organic solvents. Recently, the concept of internal gelation has been applied to formulating nanoparticles as drug delivery systems. Emulsification/internal gelation technologies available for microparticles preparation, particularly that involving alginate polymer, are described as well as recent advances towards applications in nanotechnology. Those methods show great promise as a tool for the development of encapsulation processes, especially for the new field of nanotechnology using natural polymers.
Antibiotic Overproduction in Streptomyces coelicolor A3(2) Mediated by Phosphofructokinase Deletion*

Streptomycetes are exploited for production of a wide range of secondary metabolites, and there is much interest in enhancing the level of production of these metabolites. Secondary metabolites are synthesized in dedicated biosynthetic routes, but precursors and co-factors are derived from the primary metabolism. High level production of antibiotics in streptomycetes therefore requires engineering of the primary metabolism. Here we demonstrate this by targeting a key enzyme in glycolysis, phosphofructokinase, leading to improved antibiotic production in Streptomyces coelicolor A3(2). Deletion of pfkA2 (SCO5426), one of three annotated pfkA homologues in S. coelicolor A3(2), resulted in a higher production of the pigmented antibiotics actinorhodin and undecylprodigiosin. The pfkA2 deletion strain had an increased carbon flux through the pentose phosphate pathway, as measured by 13C metabolic flux analysis, establishing the ATP-dependent PfkA2 as a key player in determining the carbon flux distribution. The increased pentose phosphate pathway flux appeared largely because of accumulation of glucose 6-phosphate and fructose 6-phosphate, as experimentally observed in the mutant strain. Through genome-scale metabolic model simulations, we predicted that decreased phosphofructokinase activity leads to an increase in pentose phosphate pathway flux and in flux to pigmented antibiotics and pyruvate. Integrated analysis of gene expression data using a genome-scale metabolic model further revealed transcriptional changes in genes encoding redox co-factor-dependent enzymes as well as those encoding pentose phosphate pathway enzymes and enzymes involved in storage carbohydrate biosynthesis.
Oligomerization of the Fes Tyrosine Kinase

The c-fes proto-oncogene encodes a non-receptor tyrosine kinase (Fes) that has been implicated in cytokine receptor signal transduction and myeloid differentiation. Previous work from our laboratory has shown that Fes autophosphorylates via an intermolecular mechanism more commonly associated with growth factor receptor tyrosine kinases. Analysis of the Fes amino acid sequence with the COILS algorithm indicates that the N-terminal region of the protein has a very high probability of forming coiled-coil structures often associated with oligomeric proteins. These findings suggest that oligomerization may be a prerequisite for trans-autophosphorylation and activation of Fes. To establish whether the active form of Fes is oligomeric, we performed gel-filtration experiments with recombinant Fes and found that it eluted as a single symmetrical peak of approximately 500 kDa. No evidence of the monomeric, 93-kDa form of the protein was observed. Deletion of the unique N-terminal domain (amino acids 1–450, including the coiled-coil homology region) completely abolished the formation of oligomers. Furthermore, co-precipitation assays demonstrated that an immobilized glutathioneS-transferase fusion protein containing the Fes N-terminal region bound to full-length Fes but not to a mutant lacking the N-terminal region. Similarly, a recombinant Fes N-terminal domain protein was readily cross-linked in vitro, whereas the SH2 and kinase domains were refractory to cross-linking. Incubation of wild-type Fes with a kinase-inactive Fes mutant or with the isolated N-terminal region suppressed Fes autophosphorylation in vitro, suggesting that oligomerization may be essential for autophosphorylation of full-length Fes. The presence of an oligomerization function in the Fes family of tyrosine kinases suggests a novel mechanism for non-receptor protein-tyrosine kinase regulation.
An Investigation into the Antecedents of Organizational Citizenship Behaviors in a Personal Selling Context

The authors report the results of two studies that attempt to model antecedents of organizational citizenship behaviors in a personal selling context. They draw the antecedents from extant research and propose that the willingness to perform organizational citizenship behaviors is related to the job-related perceptions of the degree of organizational fit between the salesperson and his or her firm, level of leadership support, perceived fairness in reward allocation (i.e., distributive justice), and job satisfaction. They hypothesize and test direct and indirect relations with these constructs and organizational citizenship behaviors. Most of these relations were significant across the two studies.
Equality, diversity and corporate responsibility

Purpose – The purpose of this paper is to explore the triggers to the development of sexual orientation diversity policy and practice in the UK private sector, based on the perspectives of those “championing” sexual orientation diversity work.Design/methodology/approach – The paper is based on 22 in‐depth key informant interviews which can be broken down as follows: diversity specialists (5), management (6, of whom 3 were sexual orientation senior management “champions” in their organisations), trade union (3) and LGBT network group representatives (8), as well as access to company and trade union web sites and publications. Interviews sought to trace the history of sexual orientation equality and diversity work, development of structures, policies and practices as well as triggers and barriers to progress and areas of innovation.Findings – Recent literature on equality and diversity in the British national context has sought to explore the implications of a social justice versus a business case driven eq.
Governance of Intercollegiate Athletics: Perceptions of Faculty Members

This study examined faculty perceptions of (a) the impact of intercollegiate athletics on institutional goals and educational experiences, (b) the role and influence of faculty regarding athletics, and (c) the factors influencing their willingness (or unwillingness) to participate in the governance of intercollegiate athletics. The study also investigated differences in faculty reactions among subgroups defined by gender, faculty status, and previous athletic participation. A sample of 240 faculty from a Big Ten Conference university responded to a mailed questionnaire. Factor analyses yielded 2 factors in impact of intercollegiate athletics, 2 factors in role and influence, 3 factors in reasons for faculty involvement, and 6 factors in reasons for lack of faculty involvement. MANOVA results indicated that those who had participated in athletics perceived greater Educational Contribution of athletics and less Value Conflicts between athletics and academics than those who had not participated in athletics.
Erectile Dysfunction and Cardiovascular Events in Diabetic Men: A Meta-analysis of Observational Studies

Background Several studies have shown that erectile dysfunction (ED) influences the risk of cardiovascular events (CV events). However, a meta-analysis of the overall risk of CV events associated with ED in patients with diabetes has not been performed. Methodology/Principal Findings We searched MEDLINE and the Cochrane Library for pertinent articles (including references) published between 1951 and April 22, 2012. English language reports of original observational cohort studies and cross-sectional studies were included. Pooled effect estimates were obtained by random effects meta-analysis. A total of 3,791 CV events were reported in 3 cohort studies and 9 cross-sectional studies (covering 22,586 subjects). Across the cohort studies, the overall odds ratio (OR) of diabetic men with ED versus those without ED was 1.74 (95% confidence interval [CI]: 1.34–2.27; P<0.001) for CV events and 1.72 (95% CI: 1.5–1.98; P<0.001) for coronary heart disease (CHD). The funnel plot, Begg's test, and Egger's test did not show evidence of publication bias (all P>0.05). Moreover, meta-regression analysis found no relationship between the method used to assess ED (questionnaire or interview), mean age, mean hemoglobin A1c, mean body mass index, or mean duration of diabetes and the risk of CV events or CHD. In the cross-sectional studies, the OR of diabetic men with ED versus those without ED was 3.39 (95% CI: 2.58–4.44; P<0.001) for CV events (N = 9), 3.43 (95% CI: 2.46–4.77; P<0.001) for CHD (N = 7), and 2.63 (95% CI: 1.41–4.91; P = 0.002) for peripheral vascular disease (N = 5). Conclusion/Significance ED was associated with an increased risk of CV events in diabetic patients. Prevention and early detection of cardiovascular disease are important in the management of diabetes, especially in view of the rapid increase in its prevalence.
Referrals: Peer Screening and Enforcement in a Consumer Credit Field Experiment

Empirical evidence on peer intermediation lags behind many years of lending practice and a large body of theory in which lenders use peers to mitigate adverse selection and moral hazard. Using a simple referral incentive mechanism under individual liability, we develop and implement a two-stage field experiment that permits separate identification of peer screening and enforcement effects. We allow for borrower heterogeneity in both ex-ante repayment type and ex-post susceptibility to social pressure. Our key contribution is how we deal with the interaction between these two sources of asymmetric information. Our method allows us to identify selection on the likelihood of repayment, selection on the susceptibility to social pressure, and loan enforcement. We estimate peer effects on loan repayment in our setting, and find no evidence of screening (albeit with an imprecisely estimated zero) and large effects on enforcement. We then discuss the potential utility and portability of the methodological innovation, for both science and for practice.
Why Chunking Should be Considered as an Explanation for Developmental Change before Short-Term Memory Capacity and Processing Speed

The chunking hypothesis suggests that during the repeated exposure of stimulus material, information is organized into increasingly larger chunks. Many researchers have not considered the full power of the chunking hypothesis as both a learning mechanism and as an explanation of human behavior. Indeed, in developmental psychology there is relatively little mention of chunking and yet it can be the underlying cause of some of the mechanisms of development that have been proposed. This paper illustrates the chunking hypothesis in the domain of non-word repetition, a task that is a strong predictor of a child’s language learning. A computer simulation of non-word repetition that instantiates the chunking mechanism shows that: (1) chunking causes task behavior to improve over time, consistent with children’s performance; and (2) chunking causes perceived changes in areas such as short-term memory capacity and processing speed that are often cited as mechanisms of child development. Researchers should be cautious when considering explanations of developmental data, since chunking may be able to explain differences in performance without the need for additional mechanisms of development.
Investigating the dynamic role of fluctuations in ongoing activity in the human brain

Traditionally, the focus in cognitive neuroscience has been on so-called evoked neural activity in response to certain stimuli or experiences. However, most of the brain’s activity is actually spontaneous and therefore not ascribed to the processing of a certain task or stimulus – or in other words, uncoupled to overt stimuli or motor outputs. In this thesis I investigated the functional role of spontaneous activity with a focus on its role in contextual changes ranging from recent experiences of individuals to trial-by-trial variability in a certain task. I studied the nature of ongoing activity from two perspectives: One looking at changes in the ongoing activity due to learning, and the other one looking at the predictive role of prestimulus activity using different methodologies, i.e. EEG and fMRI. Finally, I ventured into the realm of inter-individual differences and mind-wandering to investigate the relationship between ongoing activity, certain behavioural traits and neuronal connectivity.
Evolution of the Borrelia burgdorferi outer surface protein OspC

The genes coding for outer surface protein OspC from 22 Borrelia burgdorferi strains isolated from patients with Lyme borreliosis were cloned and sequenced. For reference purposes, the 16S rRNA genes from 17 of these strains were sequenced after being cloned. The deduced OspC amino acid sequences were aligned with 12 published OspC sequences and revealed the presence of 48 conserved amino acids. On the basis of the alignment, OspC could be divided into an amino-terminal relatively conserved region and a relatively variable region in the central portion. The distance tree obtained divided the ospC sequences into three groups. The first group contained ospC alleles from all (n = 13) sensu stricto strains, the second group contained ospC alleles from seven Borrelia afzelii strains, and the third group contained ospC alleles from five B. afzelii and all (n = 9) Borrelia garinii strains. The ratio of the mean number of synonymous (dS) and nonsynonymous (dN) nucleotide substitutions per site calculated for B. burgdorferi sensu stricto, B. garinii, and B. afzelii ospC alleles suggested that the polymorphism of OspC is due to positive selection favoring diversity at the amino acid level in the relatively variable region. On the basis of the comparison of 16S rRNA gene sequences, Borrelia hermsii is more closely related to B. afzelii than to B. burgdorferi sensu stricto and B. garinii. In contrast, the phylogenetic tree obtained for the B. hermsii variable major protein, Vmp33, and 18 OspC amino acid sequences suggested that Vmp33 and OspC from B. burgdorferi sensu stricto strains share a common evolutionary origin.