Miracle-dz/UniFAR-FTUs huggingface dataset:
Recombinant TIGR/MYOC increases outflow resistance in the human anterior segment.

PURPOSE To determine the effect of human recombinant TIGR/myocilin (MYOC) protein on outflow resistance in the human anterior segment. METHODS A cDNA for MYOC was inserted into a bacterial expression system and purified with nickel ion affinity chromatography. The anterior segments of 12 pairs of human eyes were placed in perfusion organ culture. One eye received an anterior chamber exchange with partially purified recombinant MYOC (25 microgram), whereas the other eye received either heat-denatured recombinant MYOC (25 microgram), partially purified ss-galactosidase (25 or 250 microgram), or partially purified control proteins isolated from a null expression lysate (25 microgram). Eyes were fixed up to 72 hours after infusion, and immunohistochemistry was performed using anti-MYOC polyclonal antibody. RESULTS Recombinant MYOC caused an increase in IOP over 12 hours, increasing outflow resistance 94%, whereas the fellow eye infused with null expression sample increased 12% (n = 7; P = 0.0005). When compared with recombinant MYOC, neither heat-denatured MYOC, recombinant ss-galactosidase, bovine serum albumin, nor fetal calf serum caused an increase in outflow resistance. MYOC IOP remained above baseline levels for 48 to 72 hours. Immunohistochemistry results confirmed the presence of recombinant MYOC in the trabecular meshwork. CONCLUSIONS Recombinant MYOC increased outflow resistance in human anterior segments, whereas control proteins did not. MYOC may increase outflow resistance by specific interactions within the trabecular meshwork.
Seagrasses along the Sicilian coasts

All seagrass species known from the Mediterranean basin have been recorded along the Sicilian coast, where studies have been carried out at a very local scale and information is fragmented or confined to the grey literature. The objective of this article is to summarise and evaluate current knowledge on seagrass species on the Sicilian coasts, providing an overview of species distribution, genetic diversity, biology and ecology, based on the literature and unpublished data. Most literature studies have been carried out on Posidonia oceanica meadows because of their wide distribution, complexity and ecological importance. In this study, the analyses carried out on P. oceanica structural and functional features show that the Sicilian meadows are in good condition with respect to the Mediterranean average, probably because of relatively low anthropogenic pressure and favourable ecological conditions. The available data on this species summarised in this article represent an important starting point from which to build effective plans for understanding levels of environmental threats and for supporting conservation strategies for these important ecosystems. Conversely, the limited information available on other seagrasses only allows the description of some structural and functional features, and does not permit to drive overall conclusions on their general health status.
Biodegradable micelles enhance the antiglioma activity of curcumin in vitro and in vivo

Curcumin (Cur), a natural polyphenol of Curcuma longa, has been recently reported to possess antitumor activities. However, due to its poor aqueous solubility and low biological availability, the clinical application of Cur is quite limited. The encapsulation of hydrophobic drugs into nanoparticles is an effective way to improve their pharmaceutical activities. In this research, nanomicelles loaded with Cur were formulated by a self-assembly method with biodegradable monomethoxy poly(ethylene glycol)-poly(lactide) copolymers (MPEG-PLAs). After encapsulation, the cellular uptake was increased and Cur could be released from MPEG-PLA micelles in a sustained manner. The Cur-loaded MPEG-PLA micelles (Cur/MPEG-PLA micelles) exhibited an enhanced toxicity on C6 and U251 glioma cells and induced more apoptosis on C6 glioma cells compared with free Cur. Moreover, the therapy efficiency of Cur/MPEG-PLA micelles was evaluated at length on a nude mouse model bearing glioma. The Cur/MPEG-PLA micelles were more effective on suppressing tumor growth compared with free Cur, which indicated that Cur/MPEG-PLA micelles improved the antiglioma activity of Cur in vivo. The results of immunohistochemical and immunofluorescent analysis indicated that the induction of apoptosis, antiangiogenesis, and inhibition of cell proliferation may contribute to the improvement in antiglioma effects. Our data suggested that Cur/MPEG-PLA may have potential clinic applications in glioma therapy.
Behaviorist Thinking in Judgments of Wrongness, Punishment, and Blame

Moral judgment depends upon inferences about agents’ beliefs, desires, and intentions. Here, we argue that in addition to these factors, people take into account the moral optimality of an action. Three experiments show that even agents who are ignorant about the nature of their moral decisions are held accountable for the quality of their decision—a kind of behaviorist thinking, in that such reasoning bypasses the agent’s mental states. In particular, whereas optimal choices are seen as more praiseworthy than suboptimal choices, decision quality has no further effect on moral judgments—a highly suboptimal choice is seen as no worse than a marginally suboptimal choice. These effects held up for judgments of wrongness and punishment (Experiment 1), positive and negative outcomes (Experiment 2), and agents with positive and negative intentions (Experiment 3). We argue that these results reflect a broader tendency to irresistibly apply the Efficiency Principle when explaining behavior.
Preschoolers' and Chimpanzees' Use of Source Reliability on Action-Based Tasks

One way to optimize social learning is to be selective when choosing from what sources to accept information. Preschoolers prefer to learn from previously accurate or competent sources, rather than from unreliable ones (e.g., Koenig, Clement, & Harris, 2004). The current study extends this work by comparing the ability to monitor an actor’s success in two species: children and chimpanzees. Members of both species saw two actors try to open containers, with different outcomes. Then, a forced-choice response was used to determine whether participants would pair the container with the previously successful actor. While preschoolers correctly elicited help from a previously successful actor, chimpanzees did not reliably select the type of object the actor could open. The current findings suggest a difference between humans and chimpanzees’ use of past source reliability, which may reflect or result from differences in their use of social learning.
Evaluation of ELISA and haemagglutination inhibition as screening tests in serosurveillance for H5/H7 avian influenza in commercial chicken flocks

Abstract Avian influenza virus (AIV) subtypes H5 and H7 can infect poultry causing low pathogenicity (LP) AI, but these LPAIVs may mutate to highly pathogenic AIV in chickens or turkeys causing high mortality, hence H5/H7 subtypes demand statutory intervention. Serological surveillance in the European Union provides evidence of H5/H7 AIV exposure in apparently healthy poultry. To identify the most sensitive screening method as the first step in an algorithm to provide evidence of H5/H7 AIV infection, the standard approach of H5/H7 antibody testing by haemagglutination inhibition (HI) was compared with an ELISA, which detects antibodies to all subtypes. Sera (n = 1055) from 74 commercial chicken flocks were tested by both methods. A Bayesian approach served to estimate diagnostic test sensitivities and specificities, without assuming any ‘gold standard’. Sensitivity and specificity of the ELISA was 97% and 99.8%, and for H5/H7 HI 43% and 99.8%, respectively, although H5/H7 HI sensitivity varied considerably between infected flocks. ELISA therefore provides superior sensitivity for the screening of chicken flocks as part of an algorithm, which subsequently utilises H5/H7 HI to identify infection by these two subtypes. With the calculated sensitivity and specificity, testing nine sera per flock is sufficient to detect a flock seroprevalence of 30% with 95% probability.
How to Select for Preoperative Short-course Radiotherapy, While Considering Long-course Chemoradiotherapy or Immediate Surgery, and Who Benefits?

© TOUCH MEDICAL MEDIA 2014 17 Abstract The management of patients with locally advanced rectal cancer (LARC) has evolved with the aim of reducing local recurrence and improving survival. Current practice has developed from refinements in surgical technique, the availability of different types of preoperative imaging, the selective or blanket use of neoadjuvant treatment (usually radiation) and sophisticated efforts exploring multimodality treatments to achieve organ preservation. Both short-course preoperative radiotherapy (SCPRT) and long-course chemoradiation (CRT) are considered standard neoadjuvant strategies, which are advocated in different parts of the world. New techniques in the delivery of radiotherapy, such as intensity-modulated radiotherapy (IMRT), may allow more precise dosing to the target volume (tumour and/or locoregional lymph nodes) and limit radiation doses to critical normal structures; however, current schedules of SCPRT and CRT impact on late function, and if they do not improve survival in resectable cancers, can they be omitted in selected cases?
Detection of Diffuse Interstellar [O II] Emission from the Milky Way Using Spatial Heterodyne Spectroscopy

Using a newly developed spatial heterodyne spectrometer (SHS), we have obtained the first radial velocity resolved emission-line profiles of diffuse [O II] 3726 and 3729 Å emission lines from the warm (104 K) ionized component of our Galaxy's interstellar medium. These [O II] lines are a principal coolant for this widespread, photoionized gas and are a potential tracer of variations in the gas temperature resulting from unidentified heating processes that appear to be acting within the Galaxy's disk and halo. By spectrally isolating for the first time Galactic [O II] from atmospheric [O II] emission, we were able to detect interstellar [O II] out to 20° from the Galactic equator with intensities that range from tens of rayleighs near the Galactic plane to less than 1 rayleigh at high Galactic latitudes. The [O II] line profiles clearly show structure indicating emission along the lines of sight from both local and more distant interstellar gas. Comparisons of the [O II] intensities with the intensities of [N II] 6584 Å and Hα 6563 Å observed with WHAM indicate that the observed variations in [N II]/Hα and [O II]/Hα in the diffuse interstellar gas are consistent with variations in temperature and confirm the value of the [O II] observations as a temperature diagnostic for the WIM.
Competition Policy and Exit Rates: Evidence from Switzerland

Abstract This paper provides evidence on the relation between the intensity of product-market competition and the probability of exit. We adopt a natural experiment approach to analyze the impact of a tightening of Swiss antitrust legislation on exit probabilities. Based on a sample of more than 68,000 firms from all major sectors of the Swiss economy, we find that the exit probability of non-exporting firms increased significantly, whereas the exit probability of exporting firms remained largely unaffected. Our results support the notion that there is a positive relationship between the intensity of product-market competition and the probability of exit.
Prenatal exposure to selective serotonin reuptake inhibitors and social responsiveness symptoms of autism: population-based study of young children

Background Selective serotonin reuptake inhibitors (SSRIs) are considered safe and are frequently used during pregnancy. However, two case–control studies suggested an association between prenatal SSRI exposure with childhood autism. Aims To prospectively determine whether intra-uterine SSSRI exposure is associated with childhood autistic symptoms in a population-based study. Method A total of 376 children prenatally exposed to maternal depressive symptoms (no SSRI exposure), 69 children prenatally exposed to SSRIs and 5531 unexposed children were included. Child pervasive developmental and affective problems were assessed by parental report with the Child Behavior Checklist at ages 1.5, 3 and 6. At age 6, we assessed autistic traits using the Social Responsiveness Scale (n = 4264). Results Prenatal exposure to maternal depressive symptoms without SSRIs was related to both pervasive developmental (odds ratio (OR) = 1.44, 95% CI 1.07–1.93) and affective problems (OR = 1.44, 95% CI 1.15–1.81). Compared with unexposed children, those prenatally exposed to SSRIs also were at higher risk for developing pervasive developmental problems (OR = 1.91, 95% CI 1.13–3.47), but not for affective problems. Children prenatally exposed to SSRIs also had more autistic traits (B = 0.15, 95% CI 0.08–0.22) compared with those exposed to depressive symptoms only. Conclusions Our results suggest an association between prenatal SSRI exposure and autistic traits in children. Prenatal depressive symptoms without SSRI use were also associated with autistic traits, albeit this was weaker and less specific. Long-term drug safety trials are needed before evidence-based recommendations are possible.
Optimal Taxation with Endogenous Insurance Markets

We study optimal tax policy in a dynamic private information economy with endogenous private markets. We characterize efficient allocations and competitive equilibria. A standard assumption in the literature is that trades are observable by all agents. We show that in such an environment the competitive equilibrium is efficient. The only effect of government interventions is crowding out of private insurance. We then relax the assumption of observability of consumption and consider an environment with unobservable trades in competitive markets. We show that efficient allocations have the property that the marginal product of capital is different from the market interest rate associated with unobservable trades. In any competitive equilibrium without taxation, the marginal product of capital and the market interest rate are equated, so that competitive equilibria are not efficient. Taxation of capital income can be welfare-improving because such taxation introduces a wedge between market interest rates and the marginal product of capital and allows agents to obtain better insurance in private markets. Finally, we use plausibly calibrated numerical examples to compute optimal taxes and welfare gains and compare results to an economy with a restricted set of tax instruments, and to an economy with observable trades.
Optimal Near-End Speech Intelligibility Improvement Incorporating Additive Noise and Late Reverberation Under an Approximation of the Short-Time SII

The presence of environmental additive noise in the vicinity of the user typically degrades the speech intelligibility of speech processing applications. This intelligibility loss can be compensated by properly preprocessing the speech signal prior to play-out, often referred to as near-end speech enhancement. Although the majority of such algorithms focus primarily on the presence of additive noise, reverberation can also severely degrade intelligibility. In this paper we investigate how late reverberation and additive noise can be jointly taken into account in the near-end speech enhancement process. For this effort we use a recently presented approximation of the speech intelligibility index under a power constraint, which we optimize for speech degraded by both additive noise and late reverberation. The algorithm results in time-frequency dependent amplification factors that depend on both the additive noise power spectral density as well as the late reverberation energy. These amplification factors redistribute speech energy across frequency and perform a dynamic range compression. Experimental results using both instrumental intelligibility measures as well as intelligibility listening tests show that the proposed approach improves speech intelligibility over state-of-the-art reference methods when speech signals are degraded simultaneously by additive noise and reverberation. Speech intelligibility improvements in the order of 20% are observed.
Mobile Health Apps to Facilitate Self-Care: A Qualitative Study of User Experiences

Objective Consumers are living longer, creating more pressure on the health system and increasing their requirement for self-care of chronic conditions. Despite rapidly-increasing numbers of mobile health applications (‘apps’) for consumers’ self-care, there is a paucity of research into consumer engagement with electronic self-monitoring. This paper presents a qualitative exploration of how health consumers use apps for health monitoring, their perceived benefits from use of health apps, and suggestions for improvement of health apps. Materials and Methods ‘Health app’ was defined as any commercially-available health or fitness app with capacity for self-monitoring. English-speaking consumers aged 18 years and older using any health app for self-monitoring were recruited for interview from the metropolitan area of Perth, Australia. The semi-structured interview guide comprised questions based on the Technology Acceptance Model, Health Information Technology Acceptance Model, and the Mobile Application Rating Scale, and is the only study to do so. These models also facilitated deductive thematic analysis of interview transcripts. Implicit and explicit responses not aligned to these models were analyzed inductively. Results Twenty-two consumers (15 female, seven male) participated, 13 of whom were aged 26–35 years. Eighteen participants reported on apps used on iPhones. Apps were used to monitor diabetes, asthma, depression, celiac disease, blood pressure, chronic migraine, pain management, menstrual cycle irregularity, and fitness. Most were used approximately weekly for several minutes per session, and prior to meeting initial milestones, with significantly decreased usage thereafter. Deductive and inductive thematic analysis reduced the data to four dominant themes: engagement in use of the app; technical functionality of the app; ease of use and design features; and management of consumers’ data. Conclusions The semi-structured interviews provided insight into usage, benefits and challenges of health monitoring using apps. Understanding the range of consumer experiences and expectations can inform design of health apps to encourage persistence in self-monitoring.
Comparison of Tongue and Lip Trills with Phonation of the Sustained Vowel /ε/ Regarding the Periodicity of the Electroglottographic Waveform and the Amplitude of the Electroglottographic Signal

Aim: The aim is to compare the vocal fold vibration seen during lip and tongue trills with that seen during phonation of the sustained vowel /e/, in terms of the periodicity of the EGG waveform and the amplitude of the EGG signal, in professional voice users. Study design: This was a quasi-experimental study. Methods: We used electroglottography (EGG) to compare the vocal fold vibration seen during tongue and lip trills with that seen during phonation of the sustained vowel /e/, in terms of the EGG waveform periodicity and signal amplitude, in 10 classically trained, professional singers. The participants produced the sustained vowel /e/ and performed tongue and lip trills at the same frequency and intensity. The periodicity of the waveform and the amplitude of the signal were visually analyzed by three blinded, experienced readers. To confirm the visual analysis results, we measured the jitter and shimmer of the signal and the frequency of variation in vocal fold vibration during the trill exercises. Results: The EGG waveform was classified as periodic for the sustained vowel phonation task and as quasi-periodic for the trill exercises, the vibration pattern repeating at approximately 24 Hz. Conclusion: The vibration of the vocal folds was modified according to the supraglottic movement in trills exercises.
Human caliciviruses in symptomatic and asymptomatic infections in children in Vellore, South India

Pediatric gastroenteritis is a major cause of childhood morbidity and mortality worldwide, especially in developing countries. It has been increasingly recognised that human caliciviruses (HuCV), comprising noroviruses (NoV), and sapoviruses (SaV), are important in both outbreak and non‐outbreak settings. This study aimed to characterise caliciviruses detected in the faeces of hospitalized children and children in the community in India. This study examined 350 faecal samples from children presenting to the hospital with acute gastroenteritis and 673 samples collected from children in the community, 500 from children with diarrhea, and 173 samples from children without diarrhea. Strain characterisation was performed by reverse transcription‐polymerase chain reaction (RT‐PCR) and partial sequencing of the gene encoding the RNA‐dependent RNA polymerase (RdRp) and/or a region spanning the open reading frames (ORFs) 1 and 2 (ORF1/ORF2) junction. A total of 68 of 350 specimens (19.4%) from hospitalized children were positive, and SaV and NoV accounted for 5.1 and 15.1% of the infections, respectively. Mixed infections of HuCVs with other enteric pathogens were seen in 9.4% of the total children tested. Sixty‐eight out of 673 (10.1%) samples collected from children in the community were positive for caliciviruses, and SaV and NoV accounted for 3.4 and 6.6% of the infections. In the community cohort 55/500 (11%) and 13/173 (7.5%) were from symptomatic and asymptomatic children, respectively, and SaVs accounted for 17/500 (3.4%) and NoVs for 38/500 (7.6%) of the symptomatic infections. This is the first report of genotyping of circulating caliciviruses in both hospital and community in India and has increased the evidence for the role of these viruses in pediatric gastroenteritis in India. J. Med. Virol. 79:544–551, 2007. © 2007 Wiley‐Liss, Inc.
Retaining biodiversity in intensive farmland: epiphyte removal in oil palm plantations does not affect yield

The expansion of agriculture into tropical forest frontiers is one of the primary drivers of the global extinction crisis, resulting in calls to intensify tropical agriculture to reduce demand for more forest land and thus spare land for nature. Intensification is likely to reduce habitat complexity, with profound consequences for biodiversity within agricultural landscapes. Understanding which features of habitat complexity are essential for maintaining biodiversity and associated ecosystem services within agricultural landscapes without compromising productivity is therefore key to limiting the environmental damage associated with producing food intensively. Here, we focus on oil palm, a rapidly expanding crop in the tropics and subject to frequent calls for increased intensification. One promoted strategy is to remove epiphytes that cover the trunks of oil palms, and we ask whether this treatment affects either biodiversity or yield. We experimentally tested this by removing epiphytes from four-hectare plots and seeing if the biodiversity and production of fruit bunches 2 months and 16 months later differed from equivalent control plots where epiphytes were left uncut. We found a species-rich and taxonomically diverse epiphyte community of 58 species from 31 families. Epiphyte removal did not affect the production of fresh fruit bunches, or the species richness and community composition of birds and ants, although the impact on other components of biodiversity remains unknown. We conclude that as they do not adversely affect palm oil production, the diverse epiphyte flora should be left uncut. Our results underscore the importance of experimentally determining the effects of habitat complexity on yield before introducing intensive methods with no discernible benefits.