Miracle-dz/UniFAR-FTUs huggingface dataset:
Inefficient HIV-1 trans Infection of CD4+ T Cells by Macrophages from HIV-1 Nonprogressors Is Associated with Altered Membrane Cholesterol and DC-SIGN

ABSTRACT Professional antigen-presenting cells (APC; myeloid dendritic cells [DC] and macrophages [MΦ]; B lymphocytes) mediate highly efficient HIV-1 infection of CD4+ T cells, termed trans infection, that could contribute to HIV-1 pathogenesis. We have previously shown that lower cholesterol content in DC and B lymphocytes is associated with a lack of HIV-1 trans infection in HIV-1-infected nonprogressors (NP). Here, we assessed whether HIV-1 trans infection mediated by another major APC, MΦ, is deficient in NP due to altered cholesterol metabolism. When comparing healthy HIV-1 seronegatives (SN), rapid progressors (PR), and NP, we found that monocyte-derived MΦ from NP did not mediate HIV-1 trans infection of autologous CD4+ T cells, in contrast to efficient trans infection mediated by SN and PR MΦ. MΦ trans infection efficiency was directly associated with the number of DC-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN)-expressing MΦ. Significantly fewer NP MΦ expressed DC-SIGN. Unesterified (free) cholesterol in MΦ cell membranes and lipid rafting was significantly lower in NP than PR, as was virus internalization in early endosomes. Furthermore, simvastatin (SIMV) decreased the subpopulation of DC-SIGN+ MΦ as well as cis and trans infection. Notably, SIMV decreased cell membrane cholesterol and led to lipid raft dissociation, effectively mimicking the incompetent APC trans infection environment characteristic of NP. Our data support that DC-SIGN and membrane cholesterol are central to MΦ trans infection, and a lack of these limits HIV-1 disease progression. Targeting the ability of MΦ to drive HIV-1 dissemination in trans could enhance HIV-1 therapeutic strategies. IMPORTANCE Despite the success of combination antiretroviral therapy, neither a vaccine nor a cure for HIV infection has been developed, demonstrating a need for novel prophylactic and therapeutic strategies. Here, we show that efficiency of MΦ-mediated HIV trans infection of CD4+ T cells is a unique characteristic associated with control of disease progression, and it is impaired in HIV-infected NP. In vitro treatment of MΦ from healthy donors with SIMV lowers their cholesterol content, which results in a strongly reduced trans infection ability, similar to the levels of MΦ from NP. Taken together, our data support the hypothesis that MΦ-mediated HIV-1 trans infection plays a role in HIV infection and disease progression and demonstrate that the use of SIMV to decrease this mechanism of virus transfer should be considered for future HIV therapeutic development.
Impact of Socioeconomic Status on Pretreatment Weight Loss and Survival in Non-Small-Cell Lung Cancer.

PURPOSE Socioeconomic status (SES) influences health care outcomes, but the influence of primary payer on cancer-associated wasting is unknown. We hypothesized that primary payer as an indicator of SES would influence pretreatment cancer-associated weight loss and treatment outcomes. MATERIALS AND METHODS Retrospective review of medical records identified 1,366 patients with non-small-cell lung cancer (NSCLC) consecutively treated at a tertiary care health system between January 1, 2006 and December 31, 2013. Insurance status was obtained from an institutional tumor registry. Cancer-associated weight loss was based on the validated international consensus definition of cachexia. Multivariable regression analyses were used to identify prognostic factors of pretreatment cancer-associated weight loss and survival. RESULTS The cohort included a representative group of patients with a median age at diagnosis of 64 years, 47% females, and 33% patients of nonwhite race. Pretreatment cancer-associated weight loss was present at the time of NSCLC diagnosis in 17%, 14%, 32%, and 38% of patients with stage I, II, III, and IV disease, respectively. Pretreatment cancer-associated weight loss was associated with increasing age at diagnosis, black race, single marital status, tobacco use, and disease stage. Compared with private insurance, Medicaid insurance (odds ratio, 2.17; 95% CI, 1.42 to 3.30) and lack of insurance (odds ratio, 2.32; 95% CI, 1.50 to 3.58) were associated with pretreatment cancer-associated weight loss. Among cachectic patients, comorbidity, histology, tumor grade, and disease stage were prognostic of survival on multivariable analysis; however, primary payer was not. CONCLUSION Pretreatment cancer-associated weight loss is common in patients with NSCLC, and its presence is significantly associated with lower SES. However, among patients with pretreatment cancer-associated weight loss, SES was not predictive of survival. Early use of cancer cachexia-directed therapies may improve outcomes, and further study on the biologic mechanisms of cancer cachexia will provide novel therapeutic avenues.
The effect of fatigability on Expanded Disability Status Scale components in multiple sclerosis.

Introduction The Expanded Disability Status Scale (EDSS) is the most widely used disability measure in multiple sclerosis (MS). The effect of fatigability on EDSS components has been underreported to date. Objective We investigated daytime variability in EDSS score and EDSS components – functional scores (FS) and walking distance (WD) up to 500 m, in MS patients who underwent a standardized fatiguing exercise. Methods Twenty-four patients with relapsing-remitting MS (n = 7), secondary-progressive MS (n = 8) and primary-progressive MS (n = 9) were included. Exclusion criteria were as follows: current MS relapse, infection/fever/flu-like symptoms, conditions prohibiting safe exercise testing, current medication affecting fatigue. One trained examiner performed baseline (BL) and follow-up (FU) assessments (FU1 after a standardized fatiguing exercise, FU2 after rest) over a single day. EDSS score change of ≥1 point if BL EDSS score was <5.5 or of ≥0.5 point if BL EDSS score was ≥5.5 were considered clinically meaningful. Results In progressive MS subtypes, WD decreased at FU1, but recovered at FU2, more so in secondary progressive MS subgroup with the highest BL EDSS score. Although BL EDSS scores (median, 5.0; range 4.0–6.5) and FS remained relatively stable over repeated assessments in the total group, a clinically meaningful transitory post-exercise EDSS score increase was observed in three patients with progressive MS. Conclusion WD seems to be more influenced by fatigability than the total EDSS score, more so in patients with progressive MS and higher disability. WD should be assessed after rest and this strategy should be implemented into protocols of clinical trials recruiting patients with progressive MS phenotypes.
Understanding Interleukin 33 and Its Roles in Eosinophil Development

Over the last decade, significant interest in the contribution of three “epithelial-derived cytokines,” such as thymic stromal lymphopoietin, interleukin 25, and interleukin 33 (IL-33), has developed. These cytokines have been strongly linked to the early events that occur during allergen exposures and how they contribute to the subsequent type 2 immune response. Of these three cytokines, IL-33 has proven particularly interesting because of the strong associations found between both it and its receptor, ST2, in several genome-wide association studies of allergic diseases. Further work has demonstrated clear mechanisms through which this cytokine might orchestrate allergic inflammation, including activation of several key effector cells that possess high ST2 levels, including mast cells, basophils, innate lymphoid cells, and eosinophils. Despite this, controversies surrounding IL-33 seem to suggest the biology of this cytokine might not be as simple as current dogmas suggest including: the relevant cellular sources of IL-33, with significant evidence for inducible expression in some hematopoietic cells; the mechanistic contributions of nuclear localization vs secretion; secretion and processing mechanisms; and the biological consequences of IL-33 exposure on different cell types. In this review, we will address the evidence for IL-33 and ST2 regulation over eosinophils and how this may contribute to allergic diseases. In particular, we focus on the accumulating evidence for a role of IL-33 in regulating hematopoiesis and how this relates to eosinophils as well as how this may provide new concepts for how the progression of allergy is regulated.
Improved Reversible Mapping from Color to Gray

In the reversible conversion of color images to gray ones, colors are mapped to textures and from the textures the receiver can recover the colors, i.e. one may print a color image with a black and white printer and, at a later time, recover colors. Such a method was originally devised using the wavelet transform and replacing high-frequency subbands by subsampled chrominance planes. Here, we propose to improve the original method thorough the use of a largely redundant representation of the chrominance, with multiple embedding into subbands of a general subband transform. Our results point to minimization of the variance of the error caused by noise when the chrominance is replicated into many subbands and not linearly combined. Derived noise bounds guide us on how many subbands to embed the chrominance. Experimental results were carried for multiple noise levels, models, transform sizes, and number of embedding subbands, demonstrating the theoretical analysis and the method's potential.
Foreign Ownership of U.S. Safe Assets: Good or Bad?

The last 20 years have been marked by a sharp rise in international demand for U.S. reserve assets, or safe stores-of-value. What are the welfare consequences to U.S. households of these trends, or of a reversal? In a lifecycle model with aggregate and idiosyncratic risks, the young and oldest households may benefit substantially from such capital inflows, but middle-aged savers may suffer from greater exposure to systematic risk in equity and housing markets. Under the veil of ignorance, a newborn in the lowest wealth quantile is willing to forego 2.7% of lifetime consumption to avoid a large capital outflow.
Put the Phone Down

If there ever was a key to happiness, this key would open a door that leads straight to a rich social life. And in the era of smartphones, this key to social connection is in our pockets anytime and anywhere. Or is it? Using the experience sampling method (ESM), we explore the use of computer-mediated communication (CMC) in the context of face-to-face (FtF) social interactions, testing two competing hypotheses: (1) a complementarity hypothesis stating that more channels of communication should be associated with higher well-being and (2) an interference hypothesis stating that FtF interactions could be impoverished by adding computer-mediated channels of communication. We surveyed 174 millennials (M age = 19.28; range: 17–22) 5 times a day over a period of a week (4,508 episodes). When participants reported a mix of CMC and FtF socializing in the same episode, they felt worse and less connected than when solely interacting FtF.
Healthicization and Lay Knowledge About Eating Practices in Two African American Communities

In this manuscript, we expand upon sociological research in lay knowledge about health and healthicization by examining socially mediated ways in which 40 African American adults in two communities acquired information about eating practices. Participants employed a variety of socially informed information-seeking strategies. Many, but not all, used socially prescribed sources exhorting them to maximize their own health and reported an amalgam of experiences concerning their interpretation of healthist messages. Participants variously accepted messages about healthy eating or engaged in strategies of micro-resistance that decentered and/or reinterpreted health promotion discourse. Furthermore, participants used emic community-based resources including those that prioritized familial engagement over individual responsibility in eating practices or that drew upon alternative health practices. We discuss the implications our work has for further research on healthicization and lay knowledge about eating practices, in which community members are actively engaged in meaning-making within local socio-structural contexts.
Utility of Thromboelastography versus Routine Coagulation Tests for Assessment of Hypocoagulable State in Patients Undergoing Cardiac Bypass Surgery

Introduction: Peri-operative monitoring of coagulation is important to diagnose potential cause of hemorrhage, to manage coagulopathy and guide treatment with blood products in patients undergoing cardiac surgery with cardiopulmonary bypass. This study was done to evaluate usefulness of Thromboelastography (TEG) and routine coagulation tests (RCT) in assessing hemostatic changes and predicting postoperative bleeding in patients undergoing cardiac surgery with cardiopulmonary bypass. Methods: Fifty adult patients undergoing cardiac surgery with cardiopulmonary bypass were enrolled in this prospective study. Preoperative and post-operative samples were collected for routine coagulation tests and TEG. Regression analysis and test of significance using Pearson's correlation coefficient was performed to assess correlation between routine coagulation tests and corresponding TEG parameters . Regression analysis was done to study relation between blood loss at 24 hours and various coagulation parameters. Results: The Routine coagulation test i.e. PT, INR, APTT showed no significant correlation with corresponding TEG parameters in pre-operative samples. However platelet count significantly correlated (p = 0.004) with MA values in postoperative samples. A significant correlation (p = 0.001) was seen between fibrinogen levels and alpha angles as well as with MA in both baseline preoperative and postoperative samples. TEG parameters R time and MA in postoperative samples were the only parameters that predicted bleeders with fair accuracy. Conclusion: Though the techniques of RCT and TEG are different, a few RCT e.g. platelet count and fibrinogen correlated with corresponding TEG parameters i.e. MA and Alpha angle. TEG parameters (R time and MA in postoperative samples) were able to predict blood loss better than RCT.
Children with two homes: Psychological problems in relation to living arrangements in Nordic 2- to 9-year-olds

Aim: Joint physical custody, children spending equal time in each parents’ respective home after a parental divorce, is particularly common in Nordic compared with other Western countries. Older children have been shown to fare well in this practice but for young children there are few existing studies. The aim of this paper is to study psychological problems in 2- to 9-year-old Nordic children in different family forms. Methods: Total symptom score according to the Strengths and Difficulties Questionnaire as well as scores showing externalizing problems were compared among 152 children in joint physical custody, 303 in single care and 3207 in nuclear families through multiple linear regression analyses. Results: Children in single care had more psychological symptoms than those in joint physical custody (B = 1.08; 95% CI 0.48 to 1.67) and those in nuclear families had the least reported symptoms (B = −0.53; 95% CI −0.89 to −0.17). Externalizing problems were also lower in nuclear families (B = −0.28, 95% CI −0.52 to −0.04) compared with joint physical custody after adjusting for covariates. Conclusions: Young children with non-cohabiting parents suffered from more psychological problems than those in intact families. Children in joint physical custody had a lower total problem score than those in single care after adjusting for covariates. Longitudinal studies with information on family factors before the separation are needed to inform policy of young children’s post-separation living arrangements.
Kinetics of Manganese and Iron Oxidation by Potassium Permanganate and Chlorine Dioxide

This article describes research to quantify the kinetics of Mn(II) and Fe(II) oxidation by potassium permanganate and chlorine dioxide; illustrates the effects of reduced metal ion concentration, pH, temperature, and the presence of dissolved organic carbon (DOC) on these kinetics; an investigates aspects of oxidation through modeling analyses
Estimation of wildfire size and risk changes due to fuels treatments

Human land use practices, altered climates, and shifting forest and fire management policies have increased thefrequencyoflargewildfiresseveral-fold.Mitigationofpotentialfirebehaviourandfireseverityhaveincreasinglybeen attempted through pre-fire alteration of wildland fuels using mechanical treatments and prescribed fires. Despite annual treatment of more than a million hectares of land, quantitative assessments of the effectiveness of existing fuel treatments at reducing the size of actual wildfires or how they might alter the risk of burning across landscapes are currently lacking. Here, we present a method for estimating spatial probabilities of burning as a function of extant fuels treatments for any wildland fire-affected landscape. We examined the landscape effects of more than 72000ha of wildland fuel treatments involved in 14 large wildfires that burned 314000ha of forests in nineUS states between 2002 and 2010. Fuels treatments altered the probability of fire occurrence both positively and negatively across landscapes, effectively redistributing fire risk by changing surface fire spread rates and reducing the likelihood of crowning behaviour. Trade offs are created between formation of large areas with low probabilities of increased burning and smaller, well-defined regions with reduced fire risk. Additional keywords: FARSITE, fire behaviour, fire extent, fire management, fire modelling, fire risk, fire spread.
Co-inoculation with Yeast and LAB Under Winery Conditions: Modification of the Aromatic Profile of Merlot Wines

The present work reports the impact of yeast/LAB co-inoculation on the aromatic profile of Merlot wines made in wineries. This study was carried out over two consecutive years on five Merlot wines in Bordeaux and Swiss wineries, using Saccharomyces cerevisiae and Oenococcus oeni starter cultures. Seventy aromatic compounds were quantified and, in addition, the sensory profiles of two wines were determined in order to compare the aromatic notes of the sequential and co-inoculated wines. The influence of the timing of inoculation with lactic acid bacteria (LAB) on the metabolic profile of wines was observed. It confirmed previous work carried out on a micro-scale but, for the first time, the impact of yeast/LAB co-inoculation was significantly demonstrated from a sensory point of view under winery conditions. In particular, the fruity and lactic notes, as well as the markers associated with these descriptors, such as esters and diacetyl, were altered. Co-inoculation does not always favour fruity expression, nor does it reduce the diacetyl content and lactic aroma intensity. All of the trends were observed either in the production and degradation of metabolites, or by the development of an aromatic mask over the short and long term.
Prenatal fish oil supplementation and early childhood development in the Upstate KIDS Study

Fish oil contains omega-3 fatty acids, which play a vital role in fetal growth and development. In utero exposure to omega-3 fatty acids is exclusively dependent on maternal nutrition. Previous studies have suggested that prenatal fish oil supplementation has positive impacts on child neurodevelopment later in life. This study examines the associations between fish oil supplementation both before pregnancy and throughout pregnancy and subsequent child development. Mother–child pairs from the Upstate KIDS Study, a birth cohort consisting of children born between 2008 and 2010, were included. Self-reported prenatal fish oil supplementation data were available for 5845 children (3807 singletons and 2038 twins). At multiple time points, from 4 months to 3 years of age, child development was reported by the parents on the Ages and Stages Questionnaire (ASQ). Five developmental domains were assessed: fine motor, gross motor, communication, personal–social functioning and problem solving. Generalized linear mixed models were used to estimate odds ratios (OR) while adjusting for covariates. Primary analyses showed that the risk of failing the ASQ problem-solving domain was significantly lower among children of women who took fish oil before pregnancy (OR 0.40, 95% confidence intervals (CI) 0.18–0.89) and during pregnancy (OR 0.43, 95% CI 0.22–0.83). Gender interaction was not statistically significant, although stratified results indicated stronger associations among girls. Similarly, associations were primarily among singletons. Prenatal fish oil supplementation may be beneficial in regards to neurodevelopment. Specifically, it is associated with a lower risk of failing the problem-solving domain up to 3 years of age.
Dispersal limitation in epiphytic bromeliad communities in a Costa Rican fragmented montane landscape

Abstract: Transformation of tropical forests is likely to affect seed-dispersal patterns and influence the composition of epiphytic plant communities in human-altered habitats. We tested this hypothesis by carrying out a comparative study of seed influx, survival and growth of transplanted seedlings of epiphytic bromeliads among isolated trees in six pasture areas, six forest edges and six forest sites in a montane area in Costa Rica. In total, 72 traps trapped 1285 seeds over a 2-mo period in the dry season of 2003. For all four investigated bromeliad genera, Catopsis, Guzmania, Tillandsia and Werauhia, the number of trapped seeds in each habitat followed a pattern similar to the number of fruiting individuals in the vicinity of the traps. Traps in forest edges (30) were 1.9 times more likely to collect seeds than traps at forest interiors (30) and pasture trees (12), the latter showing similar probabilities of catching seeds. After 1 y, survival and growth of 3660 transplanted seedlings from three bromeliad species was significantly higher in forest interiors, providing no explanation for the lower abundance of fruiting adults in that habitat. These results suggest that the successful establishment of epiphytic bromeliads in forest interiors is mainly dispersal-limited. If corroborated, differences in abundance among species at each habitat are likely related to differences in growth rates and reproductive success. Further studies on the growth and mortality of seedlings up to the flowering stage, however, are needed.
Experimental validation of curvature tracking with a programmable bevel-tip steerable needle

Needle steering systems are a topic of increasing research interest due to the many potential advantages associated with the ability to reach deep-seated targets while avoiding obstacles. Existing embodiments, such as those designed around a fixed bevel tip, are necessarily disruptive to the substrate, with the potential to cause a target to move away from the insertion trajectory, as well as potentially increasing the extent of tissue trauma at the needle interface, when compared to straight needles. To alleviate these issues, we proposed a biologically inspired design, which can steer without the need for duty-cycle spinning along the insertion axis or any active mechanisms at the tip. In this work, we demonstrate for the first time that our needle is able to steer within a deformable substrate, along with a user-defined trajectory in three-dimensional space. A simplified kinematic model is reported, which is subsequently used to design an adaptive strategy enabling the tracking of arbitrary curvatures along any given reference plane. Experimental results in gelatin are used to validate our model, as well as the performance of the controller under laboratory conditions.