Miracle-dz/UniFAR-FTUs huggingface dataset:
Presynaptic Membrane Retrieval and Endosome Biology: Defining Molecularly Heterogeneous Synaptic Vesicles Not All Voices Are Created Equal: Molecular Diversity of Synaptic Vesicles Endosomes and Synaptic Vesicle Recycling: Revision of a " Controversy " after Vesicle Internalization, a Growing Body o

The release and uptake of neurotransmitters by synaptic vesicles is a tightly controlled process that occurs in response to diverse stimuli at morphologically disparate synapses. To meet these architectural and functional synaptic demands, it follows that there should be diversity in the mechanisms that control their secretion and retrieval and possibly in the composition of synaptic vesicles within the same terminal. Here we pay particular attention to areas where such diversity is generated, such as the variance in exocytosis/endocytosis coupling, SNAREs defining functionally diverse synaptic vesicle populations and the adaptor-dependent sorting machineries capable of generating vesicle diversity. We argue that there are various synaptic vesicle recycling pathways at any given synapse and discuss several lines of evidence that support the role of the endosome in synaptic vesicle recycling. C hemical synapses contain discrete numbers of synaptic vesicles, which are capable of sustaining neurotransmitter release. Sustained neurotransmission occurs despite the secretory demands imposed by persistent and diverse patterns of neuronal electrical activity. Maintaining synaptic vesicle numbers requires local mechanisms to regenerate these vesicles to prevent their exhaustion, preserve plasma membrane surface area, and to maintain the molecularly distinct identity of a vesicle versus plasma membrane. Rizzoli and Betz (2005) eloquently draw a parallel between chemical neurotransmission with synapse chatter saying that some synapses " whisper, " whereas others " shout. " The " louder " the synapse, the more synaptic vesicles are required, extending from a few hundred vesicles (whisperers) to nearly thousands (shouters). This beautiful analogy implies that every syn-apse has just one " voice " or species of vesicle. Here we will present the case that synapses are more like choirs in which multiple vesicle species or " voices " contribute to the " pianissimo " or " fortissimo " parts of chemical neurotrans-mission. Synaptic terminals show a range of structural and functional differences in distinct regions of the brain, suggesting that the mechanisms for exocytosis/endocytosis coupling, as well as local vesicle recycling, may also be diverse. On one side, the Calyx of Held nerve terminal participates in fast and sustained synaptic transmission at high frequency (800 Hz), which is crucial for sound localization in the auditory brainstem (Taschenberger and von Gersdorff 2000; Borst and Soria van Hoeve 2012). The Ca-lyx of Held houses 70,000 synaptic vesicles with nearly 3000 vesicles docked per Calyx terminal. These docked vesicles are distributed across the 500 active zones that exist per Calyx …
Data is more than knowledge: implications of the reversed knowledge hierarchy for knowledge management and organizational memory

In knowledge management literature it is often pointed out that it is important to distinguish between data, information and knowledge. The generally accepted view sees data as simple facts that become information as data is combined into meaningful structures, which subsequently become knowledge as meaningful information is put into a context and when it can be used to make predictions. This view sees data as a prerequisite for information, and information as a prerequisite for knowledge. I explore the conceptual hierarchy of data, information and knowledge, showing that data emerges only after we have information, and that information emerges only after we already have knowledge. The reversed hierarchy of knowledge is shown to lead to a different approach in developing information systems that support knowledge management and organizational memory. It is also argued that this difference may have major implications for organizational flexibility and renewal.
Validation of a Prediction Rule for the Diagnosis of Rheumatoid Arthritis in Patients with Recent Onset Undifferentiated Arthritis

Objectives. To validate van der Helm-van Mil score (vHvM) and new ACR/EULAR criteria for the diagnosis of rheumatoid arthritis (RA) in patients with undifferentiated arthritis (UA). Patients and Methods. Adult patients with UA (swelling ≥2 joints of less than 6 months duration, without diagnosis, and never treated with disease modifying drugs). Results. Ninety-one patients were included. Mean age: 55.6 years (SD: 17.4), 74% females. Median symptoms duration was 2 months (IR: 1–4 months). Mean van der Helm-van Mil score was 6.9 (SD: 2). After a mean followup of 6.2 months (SD: 6), 40.7% patients fulfilled ACR 1987 RA classification criteria, 28.6% fulfilled other diagnostic criteria, and 31% remained as UA. Receiver operator characteristic curve's (ROC's) area under the curve (AUC) for the vHvM score for diagnosis of RA was 0.83. A cutoff value of 6.94 showed sensitivity of 81% and 79.7% specificity. For the new ACR/EULAR criteria, the ROC AUC was 0.93, and a value equal to or greater than 6 showed 86.5% sensitivity and 87% specificity. Conclusion. van der Helm-van Mil prediction score and the new ACR/EULAR criteria proved to be valuable for the diagnosis of RA in patients with early UA.
Minimum Wages and Aggregate Job Growth: Causal Effect or Statistical Artifact?

A recent paper by Meer and West argues that minimum wages reduce aggregate employment growth, and that this relationship is masked by looking at employment levels. I also find a negative association between minimum wages and aggregate employment growth using both the Business Dynamics Statistics and the Quarterly Census of Employment and Wages datasets, and it is sizable for some time periods. However, I show that this negative association is present in exactly the wrong sectors. It is particularly strong in manufacturing which hires very few minimum wage workers. At the same time, there is no such association in retail, or in accommodation and food services – which together hire nearly 2/3 of all minimum wage workers. These results indicate that the negative association between minimum wages and aggregate employment growth does not represent a causal relationship. Rather the association stems from an inability to account for differences between high and low minimum wage states and the timing of minimum wage increases. Consistent with that interpretation, when I use bordering counties to construct more credible control groups, I find no such negative correlation between minimum wages and overall employment growth.
Exploring perceptual matters : a textile-based approach

This research takes a practice-based approach to exploring perceptual matters that often go unnoticed in the context of everyday lived experience. My approach focuses on the experiential possibilities of knowledge emerging through artistic enquiry, and uses a variety of modes (like textiles, sound, physical computing, programming, video and text) to be conducted and communicated. It examines scholarship in line with the ecological theory of perception, and is particularly informed by neurobiological research on sensory integration as well as by cultural theories that examine the role of sensory appreciation in perception. Different processes contributing to our perceptual experience are examined through the development of a touch-sensitive, sound-generating rug and its application in an experimental context. Participants’ interaction with the rug and its sonic output allows an insight into how they make sense of multisensory information via observation of how they physically respond to it. In creating possibilities for observing the two ends of the perceptual process (sensory input and behavioural output), the rug provides a platform for the study of what is intangible to the observer (perceptual activity) through what can actually be observed (physical activity). My analysis focuses on video recordings of the experimental process and data reports obtained from the software used for the sound generating performance of the rug. Its findings suggest that attentional focus, active exploration, and past experience actively affect the ability to integrate multisensory information and are crucial parameters for the formation of a meaningful percept upon which to act. Although relational to the set experimental conditions and the specificities of the experimental group, these findings are in resonance with current cross-disciplinary discourse on perception, and indicate that art research can be incorporated into the wider arena of neurophysiological and behavioural research to expand its span of resources and methods.
Swedish active labour market programmes in the 1990s: Overall effectiveness and differential performance

The ? wedish model' of active labour market programmes is investigated in relation to some crucial institutional features with two aims: examining how successful it has been in the context of the high unemployment atypically experienced by Sweden in the 1990s and trying to derive some general lessons as to which type of programme works best. The effectiveness of the programmes in improving the labour market prospects of unemployed participants is assessed in terms of their impact on individual employment probability and collection of unemployment benefits over time. The evidence as to the overall effectiveness of the programmes is rather mixed, with individuals joining a programme subsequently enjoying higher employment rates but also a higher probability of drawing unemployment benefits over time than if they had searched longer in open unemployment. The renewed eligibility to unemployment compensation following participation in a programme appears to be a most critical driving force behind these results. In fact, when comparing the programme effects for individuals entitled to unemployment benefits to the programme effects for non-entitled individuals, the positive effect on participants' employment prospects disappears, being instead replaced by a much higher probability of benefit collection. Still, the various programmes may have differential effects, making it interesting to quantify the relative performance of the six main types of Swedish programmes that were available to adult unemployed workers en Titled to unemployment benefits in the 1990s: labour market training, workplace introduction, work experience placement, relief work, trainee replacement and employment subsidies. The best performer is by far employment subsidies, followed by trainee replacement. The main finding that those programmes most similar to regular employment rank unambiguously highest has however to be appraised in the light of the macroeconomic literature, which has documented large and negative displacement and dead-weight effects exactly for these types of programme.
Investigation of Lifetime-Limiting Defects After High-Temperature Phosphorus Diffusion in High-Iron-Content Multicrystalline Silicon

Phosphorus diffusion gettering of multicrystalline silicon solar cell materials generally fails to produce material with minority-carrier lifetimes that approach that of gettered monocrystalline wafers, due largely to higher levels of contamination with metal impurities and a higher density of structural defects. Higher gettering temperatures should speed the dissolution of precipitated metals by increasing their diffusivity and solubility in the bulk, potentially allowing for improved gettering. In this paper, we investigate the impact of gettering at higher temperatures on low-purity multicrystalline samples. To analyze the gettering response, we measure the spatially resolved lifetime and interstitial iron concentration by microwave photoconductance decay and photoluminescence imaging, and the structural defect density by Sopori etching and large-area automated quantification. Higher temperature phosphorus diffusion gettering is seen to improve metal-limited multicrystalline materials dramatically, especially in areas of low etch pit density. In areas of high as-grown dislocation density in the multicrystalline materials, it appears that higher temperature phosphorus diffusion gettering reduces the etch pit density, but leaves higher local concentrations of interstitial iron, which degrade lifetime.
Beyond millisecond latency kNN search on commodity machine

To the best of our knowledge, this is the first work that offers very low latency (0.1ms) per kNN query on 10-million-node networks on a commodity machine. This translates to a query throughput of 10,000 queries per second per commodity machine. Our experimental studies on large scale road networks show that our solutions are 1–3 orders of magnitudes faster than existing methods while our indexes are compact and can fit into main memory. In the future, we plan to further enhance the performance of kNN search with keywords.
Treatment outcome in adults with chronic fatigue syndrome: a prospective study in England based on the CFS/ME National Outcomes Database

Background: Chronic fatigue syndrome (CFS) is relatively common and disabling. Over 8000 patients attend adult services each year, yet little is known about the outcome of patients attending NHS services. Aim: Investigate the outcome of patients with CFS and what factors predict outcome. Design: Longitudinal patient cohort. Methods: We used data from six CFS/ME (myalgic encephalomyelitis) specialist services to measure changes in fatigue (Chalder Fatigue Scale), physical function (SF-36), anxiety and depression (Hospital Anxiety and Depression Scale) and pain (visual analogue pain rating scale) between clinical assessment and 8–20 months of follow-up. We used multivariable linear regression to investigate baseline factors associated with outcomes at follow-up. Results: Baseline data obtained at clinical assessment were available for 1643 patients, of whom 834 (51%) had complete follow-up data. There were improvements in fatigue [mean difference from assessment to outcome: −6.8; 95% confidence interval (CI) −7.4 to −6.2; P < 0.001]; physical function (4.4; 95% CI 3.0–5.8; P < 0.001), anxiety (−0.6; 95% CI −0.9 to −0.3; P < 0.001), depression (−1.6; 95% CI −1.9 to −1.4; P < 0.001) and pain (−5.3; 95% CI −7.0 to −3.6; P < 0.001). Worse fatigue, physical function and pain at clinical assessment predicted a worse outcome for fatigue at follow-up. Older age, increased pain and physical function at assessment were associated with poorer physical function at follow-up. Conclusions: Patients who attend NHS specialist CFS/ME services can expect similar improvements in fatigue, anxiety and depression to participants receiving cognitive behavioural therapy and graded exercise therapy in a recent trial, but are likely to experience less improvement in physical function. Outcomes were predicted by fatigue, disability and pain at assessment.
Ex Vivo-Generated CD36+ Erythroid Progenitors Are Highly Permissive to Human Parvovirus B19 Replication

ABSTRACT The pathogenic parvovirus B19 (B19V) has an extreme tropism for human erythroid progenitor cells. In vitro, only a few erythroid leukemic cell lines (JK-1 and KU812Ep6) or megakaryoblastoid cell lines (UT7/Epo and UT7/Epo-S1) with erythroid characteristics support B19V replication, but these cells are only semipermissive. By using recent advances in generating large numbers of human erythroid progenitor cells (EPCs) ex vivo from hematopoietic stem cells (HSCs), we produced a pure population of CD36+ EPCs expanded and differentiated from CD34+ HSCs and assessed the CD36+ EPCs for their permissiveness to B19V infection. Over more than 3 weeks, cells grown in serum-free medium expanded more than 800,000-fold, and 87 to 96% of the CD36+ EPCs were positive for globoside, the cellular receptor for B19V. Immunofluorescence (IF) staining showed that about 77% of the CD36+ EPCs were positive for B19V infection, while about 9% of UT7/Epo-S1 cells were B19V positive. Viral DNA detected by real-time PCR increased by more than 3 logs in CD36+ EPCs; the increase was 1 log in UT7/Epo-S1 cells. Due to the extensive permissivity of CD36+ EPCs, we significantly improved the sensitivity of detection of infectious B19V by real-time reverse transcription-PCR and IF staining 100- and 1,000-fold, respectively, which is greater than the sensitivity of UT7/Epo-S1 cell-based methods. This is the first description of an ex vivo method to produce large numbers of EPCs that are highly permissive to B19V infection and replication, offering a cellular system that mimics in vivo infection with this pathogenic human virus.
Multilingual Sentiment Analysis using Machine Translation?

The past years have shown a steady growth in interest in the Natural Language Processing task of sentiment analysis. The research community in this field has actively proposed and improved methods to detect and classify the opinions and sentiments expressed in different types of text - from traditional press articles, to blogs, reviews, fora or tweets. A less explored aspect has remained, however, the issue of dealing with sentiment expressed in texts in languages other than English. To this aim, the present article deals with the problem of sentiment detection in three different languages - French, German and Spanish - using three distinct Machine Translation (MT) systems - Bing, Google and Moses. Our extensive evaluation scenarios show that SMT systems are mature enough to be reliably employed to obtain training data for languages other than English and that sentiment analysis systems can obtain comparable performances to the one obtained for English.
Horizontal and Vertical Distribution of Marine Virioplankton: A Basin Scale Investigation Based on a Global Cruise

Despite the fact that marine viruses have been increasingly studied in the last decade, there is little information on viral abundance and distribution on a global scale. In this study, we report on a global-scale survey covering the Pacific, Atlantic, and Indian Oceans on viral distribution using flow cytometry. Viruses were stained with the SYBR Green I, which targets only dsDNA viruses. The average viral abundance was 1.10±0.73×107 ml−1 in global surface oceans and decreased from the areas with high chlorophyll concentration (on average, 1.47±0.78×107 ml−1) to the oligotrophic subtropical gyres (on average, 6.34±2.18×106 ml−1). On a large-spatial-scale, viruses displayed significant relationships with both heterotrophic and autotrophic picoplankton abundance, suggesting that viral distribution is dependent on their host cell abundance. Our study provided a basin scale pattern of marine viral distributions and their relationship with major host cells, indicating that viruses play a significant role in the global marine ecosystem.
New Clathrin-Based Nanoplatforms for Magnetic Resonance Imaging

Background Magnetic Resonance Imaging (MRI) has high spatial resolution, but low sensitivity for visualization of molecular targets in the central nervous system (CNS). Our goal was to develop a new MRI method with the potential for non-invasive molecular brain imaging. We herein introduce new bio-nanotechnology approaches for designing CNS contrast media based on the ubiquitous clathrin cell protein. Methodology/Principal Findings The first approach utilizes three-legged clathrin triskelia modified to carry 81 gadolinium chelates. The second approach uses clathrin cages self-assembled from triskelia and designed to carry 432 gadolinium chelates. Clathrin triskelia and cages were characterized by size, structure, protein concentration, and chelate and gadolinium contents. Relaxivity was evaluated at 0.47 T. A series of studies were conducted to ascertain whether fluorescent-tagged clathrin nanoplatforms could cross the blood brain barriers (BBB) unaided following intranasal, intravenous, and intraperitoneal routes of administration. Clathrin nanoparticles can be constituted as triskelia (18.5 nm in size), and as cages assembled from them (55 nm). The mean chelate: clathrin heavy chain molar ratio was 27.04±4.8: 1 for triskelia, and 4.2±1.04: 1 for cages. Triskelia had ionic relaxivity of 16 mM−1s−1, and molecular relaxivity of 1,166 mM−1s−1, while cages had ionic relaxivity of 81 mM−1s−1 and molecular relaxivity of 31,512 mM−1s−1. Thus, cages exhibited 20 times higher ionic relaxivity and 8,000-fold greater molecular relaxivity than gadopentetate dimeglumine. Clathrin nanoplatforms modified with fluorescent tags were able to cross or bypass the BBB without enhancements following intravenous, intraperitoneal and intranasal administration in rats. Conclusions/Significance Use of clathrin triskelia and cages as carriers of CNS contrast media represents a new approach. This new biocompatible protein-based nanotechnology demonstrated suitable physicochemical properties to warrant further in vivo imaging and drug delivery studies. Significantly, both nanotransporters crossed and/or bypassed the BBB without enhancers. Thus, clathrin nanoplatforms could be an appealing alternative to existing CNS bio-nanotechnologies.
Fault-tolerant, Scalable, Wide-Area Internet Service Composition

As the Internet rapidly evolves, a growing number of Web services begins to emerge, and the demand for accessing them through extremely diverse end devices increases. Furthermore, a need to create novel functionality (e.g., personalized speech-enabled mailbox) by composing existing services is growing. This presents an emerging opportunity for a middleware service that, given end-point specifications and desired QoS metrics, automatically composes services in a fashion similar to plugging together tinkertoy-like elements to allow service access through heterogeneous devices and networks. Services in our context are any strongly-typed, network-, and programmatically accessible applications. In this report, we present the architectural design of such a system, the implementation and performance evaluation of a prototype. We identify the key requirements of a service composition platform: automation, scalability, and fault-tolerance. We achieve these goals through use of cluster computing platforms, identification of common patterns of ad-hoc, application-specific compositions, classification of services into a strongly typed system, redundant control mechanisms for monitoring and recovery, and a continuous optimization process with feedback. One important contribution of our work is to enable creation of new functionality from existing services with minimal effort. In addition to service reuse, our system also achieves good resource utilization in the wide area by strategically placing and locating services and dynamically adapting to resource variations. We prove its usability by demonstrating the ease with which novel functionality results from existing services and good scaling performance of the system.
Short- and medium-term plasticity for speaker adaptation seem to be independent

In a classic paper, Ladefoged and Broadbent [1] showed that listeners adapt to speakers based on short-term exposure of a single phrase. Recently, Norris, McQueen, and Cutler [2] presented evidence for a lexically conditioned medium-term adaptation to a particular speaker based on an exposure of 40 critical words among 200 items. In two experiments, I investigated whether there is a connection between the two findings. To this end, a vowel-normalization paradigm (similar to [1]) was used with a carrier phrase that consisted of either words or nonwords. The range of the second formant was manipulated and this affected the perception of a target vowel in a compensatory fashion: A low F2-range made it more likely that a target vowel was perceived as a front vowel, that is, with an inherently high F2. Manipulation of the lexical status of the carrier phrase, however, did not affect vowel normalization. In contrast, the range of vowels in the carrier phrase did influence vowel normalization. If the carrier phrase consisted of high-front vowels only, vowel categories shifted only for high-front vowels. This may indicate that the short-term and medium-term adaptations are brought about by different mechanisms.