Miracle-dz/UniFAR-FTUs huggingface dataset:
MOLECULAR CHARACTERIZATION OF LARVAL ANISAKID NEMATODES FROM MARINE FISHES OF MADEIRA BY A PCR-BASED APPROACH, WITH EVIDENCE FOR A NEW SPECIES

One-hundred and fifteen anisakid larvae from 3 different fish hosts, Aphanopus carbo, Scomber japonicus, and Trachurus picturatus, caught in Madeiran waters, were identified by PCR-RFLP. Three distinct species were identified in A. carbo, namely Anisakis simplex sensu stricto, Anisakis pegreffii, and Anisakis ziphidarum; 5 in S. japonicus, i.e., A. simplex s.s. , A. pegreffii, Anisakis physeteris, Anisakis typica, and A. ziphidarum; and 3 in T. picturatus, i.e., A. simplex s.s. , A. pegreffii, and A. typica. Anisakis simplex s.s. was the most frequent species in both A. carbo and S. japonicus (54% and 23.5%, respectively). Anisakis pegreffii and A. physeteris occurred with a frequency of 20.6% in S. japonicus, whereas in T. picturatus the most frequent species was A. typica (41.9%), followed by A. simplex s.s. (32.3%). Furthermore, A. carbo and S. japonicus were infected by an apparently undescribed taxon, provisionally named Anisakis sp. A. Based on estimations of the genetic distance, this new taxon seems to be more similar to A. ziphidarum (0.0335) than to other species of the genus.
NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes

In vertebrates, the nuclear envelope (NE) assembles and disassembles during mitosis. As the NE is a complex structure consisting of inner and outer membranes, nuclear pore complexes (NPCs) and the nuclear lamina, NE assembly must be a controlled and systematic process. In Xenopus egg extracts, NE assembly is mediated by two distinct membrane vesicle populations, termed NEP-A and NEP-B. Here, we re-investigate how these two membrane populations contribute to NPC assembly. In growing stage III Xenopus oocytes, NPC assembly intermediates are frequently observed. High concentrations of NPC assembly intermediates always correlate with fusion of vesicles into preformed membranes. In Xenopus egg extracts, two integral membrane proteins essential for NPC assembly, POM121 and NDC1, are exclusively associated with NEP-B membranes. By contrast, a third integral membrane protein associated with the NPCs, gp210, associates only with NEP-A membranes. During NE assembly, fusion between NEP-A and NEP-B led to the formation of fusion junctions at which >65% of assembling NPCs were located. To investigate how each membrane type contributes to NPC assembly, we preferentially limited NEP-A in NE assembly assays. We found that, by limiting the NEP-A contribution to the NE, partially formed NPCs were assembled in which protein components of the nucleoplasmic face were depleted or absent. Our data suggest that fusion between NEP-A and NEP-B membranes is essential for NPC assembly and that, in contrast to previous reports, both membranes contribute to NPC assembly.
Introduction: Why Does Change Fail, and What Can We Do About It?

In an era when politicians can only get elected by promising change, it seems strange, as the above quote shows, that there was time when their inclination was to resist, rather than promote, change. However, the above quotation, though over 100 years old, neatly sums up many people’s attitude to organizational change: ‘we don’t like it; it’ll just make things worse’. Yet, even though many people are doubtful that change will be for the better, we live in an era where change is seen as essential if organizations and, indeed, the human race are to survive (Dunphy et al., 2007; Kanter, 2008; Sackmann et al., 2009). Such is the importance now given to change that it is seen as the prime responsibility of those who lead organizations, as the rise of the transformational leader shows (Burns, 1978; Bass, 1995; Yukl, 2010). A global survey by McKinsey & Company (2008) concluded that only by changing constantly could organizations hope to survive. There is nothing remarkable in this finding; it echoes what most writers and commentators have been saying for the past two decades (Kanter et al., 1992; Kotter, 1996; Beer and Nohria, 2000; Stacey, 2007). However, the McKinsey survey also claimed that some twothirds of all change initiatives failed, which may account for why so many people appear to have a negative perception of change. Whilst two-thirds seems Journal of Change Management Vol. 11, No. 4, 445–450, December 2011
Lives in the Making: Power, Academia and the Everyday

There has been significant attention paid to the corporatization and neoliberalization of the University as an institution in North America. In this article we examine the everyday and corporeal experiences of these processes on the lives of several graduate students in Geography PhD programs across North America. From a feminist perspective, we explore how these students become academic geographers through particular articulations of work/life embedded in complex power dynamics within the neoliberalized university. Using information gathered from in-depth interviews and open-ended survey questions we explore the contradictions inherent in academics’ efforts of finding ‘work-life balance’. Through the stories recounted, we add to the recent theorizing about neoliberalism and the academy by highlighting the ways that the neoliberalized university plays out on the everyday and bodily scale in the lives of graduate students. We seek to interrogate the making of professional geographers within these neoliberalized institutions due to a concern about the effects of this particular form of discipline and subject formation on the current and future politics of knowledge production in the field. This article is part of a multi-year project following these research participants that seeks to investigate the process of becoming geographers over time.
The Assessment of Emotional Awareness in Children Validation of the Levels of Emotional Awareness Scale for Children

The Levels of Emotional Awareness Scale (LEAS) is a widely used scenario-based instrument that has been developed for the measurement of emotional awareness in adults. Although the LEAS has been validated in numerous studies, published validity research on the recently developed child version (LEAS-C) is scarce. The objective of the present study was to evaluate the construct validity of the Dutch LEAS-C in a sample of 318 children, aged 10 to 17 years. Outcomes revealed novel structural evidence in favor of alternative design-driven modeling. Further, the pattern of relationships with ability- and trait-oriented emotional intelligence, intelligence, person- ality, social and emotional impairment, and gender was generally consistent with previous theorizing and adult studies on the LEAS. Reasons for absence of age differences are discussed. In conclusion, this study corroborates the construct validity of the LEAS-C and highlights the importance of fully exploring the LEAS-C in its potential. Directions for future research are proposed.
Combining decision trees classifiers: a case study of automatic sleep stage scoring

This paper presents a new approach of classification in which multiple decision trees are combined together for achieving better accuracy compared to that achieved by each of the individual constituent decision trees. A major unit of the proposed system is the combination unit for which we present two algorithms; one is based on pre-pruning and true positive rate and the other is based on maximum probability voting. In presenting this new method, we use the case study of sleep stage scoring as a basis of demonstration. For such a task, two tree classifications are utilised. We performed a tree classification based on the training data and then combined the resulting model with another classification tree supplemented by the expert according to Rechtschaffen and Kale's sleep scoring rules. We applied this method to nine recordings, six of which were used to construct the training tree and the remaining three were used for testing. The experiments showed that the combination method has a 7% better accuracy over a single model.
Complex and Collaborative Problem Solving: New, Unique, and Useful?

Neubert, Mainert, Kretzschmar, and Greiff (2015) suggest two individual differences that have been overlooked in the industrial–organizational (I-O) literature (i.e., complex and collaborative problem solving) will be critical in the 21st century workplace. Below, I will briefly summarize some history and empirical findings at odds with Neubert et al.’s (2015) assertions.
A Hybrid Algorithm for the University Course Timetabling Problem

In this paper, we introduce a new mathematical programming model that combines teacher assignment and course scheduling problems simultaneously. Due to the limitation of a mathematical programming approach to solve large problem instances, we propose a hybrid algorithm that combines two well known metaheuristics, Simulated Annealing (SA) and Tabu Search (TS). In the proposed algorithm, useful features of each metaheuristic are exploited to obtain better solutions. Several randomly generated problem instances are used to evaluate the performance of the proposed algorithm. The computational results illustrate the ability of the hybrid algorithm to provide good quality solutions to the problem instances within reasonable computation time.
Synonym Extraction of Medical Terms from Clinical Text Using Combinations of Word Space Models

In information extraction, it is useful to know if two signiﬁers have the same or very similar semantic content. Maintaining such information in a controlled vocabulary is, however, costly. Here it is demonstrated how synonyms of medical terms can be extracted automatically from a large corpus of clinical text using distributional semantics. By combining Random Indexing and Random Permutation, different lexical semantic aspects are captured, effectively increasing our ability to identify synonymic relations between terms. 44% of 340 synonym pairs from MeSH are successfully extracted in a list of ten suggestions. The models can also be used to map abbrevia-tions to their full-length forms; simple pattern-based ﬁltering of the suggestions yields substantial improvements.
Increased Dopamine Level Enhances Male–Male Courtship in Drosophila

Sexual behavior between males is observed in many species, but the biological factors involved are poorly known. In mammals, manipulation of dopamine has revealed the role of this neuromodulator on male sexual behavior. We used genetic and pharmacological approaches to manipulate the dopamine level in dopaminergic cells in Drosophila and investigated the consequence of this manipulation on male–male courtship behavior. Males with increased dopamine level showed enhanced propensity to court other males but did not change their courtship toward virgin females, general olfactory response, general gustatory response, or locomotor activity. Our results indicate that the high intensity of male–male interaction shown by these manipulated males was related to their altered sensory perception of other males.
Contrasting the Theory of Planned Behavior With the Value‐Belief‐Norm Model in Explaining Conservation Behavior1

In this paper, we contrast the value-belief-norm (VBN) model and the theory of planned behavior (TPB) for the first time regarding their ability to explain conservation behavior. The participants represent a convenience sample of 468 university students. Using survey data and adopting previously established compound measures, structural equation analyses revealed a remarkable explanatory power for both theories: TPB's intention accounted for 95% of people's conservation behavior and VBN's personal norms accounted for 64%. Compared to the VBN model, the TPB covered its concepts more fully in terms of proportions of explained variance. More importantly, the fit statistics revealed that only the TPB depicts the relations among its concepts appropriately, whereas the VBN model does not.
Developing interpretive power in science teaching

Early career teachers rarely receive sustained support for addressing issues of diversity and equity in their science teaching. This paper reports on design research to create a 30 hour professional development seminar focused on cultivating the interpretive power of early career teachers who teach science to students from historically non-dominant communities. Interpretive power refers to teachers’ attunement to (a) students’ diverse sense-making repertoires as intellectually generative in science and (b) expansive pedagogical practices that encourage, make visible, and intentionally build on students’ ideas, experiences, and perspectives on scientific phenomena. The seminar sought to integrate student sense-making, scientific subject matter, teaching practice, and matters of equity and diversity on the same plane of professional inquiry by engaging participants in: (a) learning plant science; (b) analyzing classroom cases; (c) experimenting with expansive discourse practices in their classrooms; and (d) analyzing their classroom experiments in relation to student sense-making and expansive pedagogy. Twenty-eight teachers participated in two cycles of design research. An interview-based transcript analysis task captured shifts in teachers’ interpretive power through their participation in the seminar. Findings showed that the teachers developed greater attunement to: complexity in students’ scientific ideas; the intellectual generativity of students’ sense-making; student talk as evidence of in-process, emergent thinking; and co-construction of meaning in classroom discussions. Findings also showed that participants developed deeper understanding of the functions of expansive teaching practices in fostering student sense-making in science and greater commitment to engaging in expansive practices in their classroom science discussions. © 2015 Wiley Periodicals, Inc. J Res Sci Teach XX:XX-XX, 2015.
Navigating the demands of the English schooling context: problematics and possibilities for social equity

The English schooling context has seen radical and rapid reform in recent times with the processes of devolution or deconcentration of centralised school governance, on the one hand, and the instating of ever-increasing and rigid external accountabilities, on the other. These reforms driven as they are by neo-liberal and neoconservative ideologies have created a new kind of ‘system’ of schooling in England, one that is ‘heterarchical’ in governance, increasingly complex in its overlap, multiplicity and asymmetric power dynamics, but one that remains strongly tied to and regulated by the reductive and narrow measures of ‘success’ imposed by the state. Against this complex and changing backdrop, what constitutes quality and equitable schooling has been transformed. This special issue explores these concerns and, in particular, focuses on how the current demands of the English schooling context construct student achievement and identity, teachers' work, conceptualisations of knowledge and pedagogy, and school organisation and collaboration. The issue has a strong equity focus. Many of the papers to this end focus on how teachers and schools are navigating through the demands of current policy reform to mobilise spaces of possibility for equity and good schooling. In this paper, we provide a context and framework to set the scene for the subsequent papers in the issue.
Pemetrexed as a component of first-, second- and third- line chemotherapy in treating patients with metastatic lung adenocarcinoma.

PURPOSE The current research was conducted to investigate the efficacy and safety of pemetrexed given continuously as a basement agent for first-, second- to third line chemotherapy of patients with metastatic lung adenocarcinoma. PATIENTS AND METHODS Patients with metastatic lung adenocarcinoma who were diagnosed in Jiangsu Cancer Hospital and Research Insitute, were enrolled. All received pemetrexed 500 mg/m2 (intravenous; on day 1), and another chemotherapieutic agent every 3 weeks until disease progression, or intolerable toxicity. Then the patients were changed to a second line chemotherapy that was still based on pemetrexed 500 mg/m2 and another chemotherapeutic agent differing from the first line example, until disease progression, or intolerable toxicity. When third line chemotherapy was needed, pemetrexed 500 mg/m2 and another new chemotherapeutic agent were combined until disease progression. Evaluation of efficacy was conducted after two cycles of chemotherapy using the Response Evaluation Criteria for Solid Tumors. Toxicity was recorded according to NCI Criteria for Adverse Events version 3.0. RESULTS From January 2010 to September 2013, 15 patients were enrolled. Their median age was 56 years (range 43 to 77 years). Eight patients were male and 7 female. Five patients (33.3%) achieved PR, while 6 patients (40.0%) remained stable, no CR on first line; and 1 PR (7.7%), 5 stable (38.5%) were recorded when pemetrexed was ordered in second line; 5 patients (41.7%) were stable after pemetrexed was combined in third line; no complete response was observed. Main side effects were grade 1 to 2 neutrophil suppression and thrombocytopenia. Other toxicities included elevated transaminase and oral mucositis, but no treatment related death occurred. CONCLUSIONS Pemetrexed continuously as a basement agent from first-, second- to third line chemotherapy is mildly effective in treating patients with metastatic lung adenocarcinoma with tolerable toxicity.
MicroRNA signatures and treatment response in patients with advanced classical Hodgkin lymphoma

Although specific microRNA (miRNA) signatures in classical Hodgkin lymphoma (cHL) have been proposed, their relationship with clinical outcome remains unclear. Despite treatment advances, a substantial subset of patients with advanced cHL are refractory to standard therapies based on adriamycin and its variants. Global miRNA expression data of 29 advanced cHL patients and five cHL‐derived cell lines were used to identify profiles from Hodgkin‐Reed‐Sternberg (HRS) cells and their non‐tumoural microenvironment. A cHL‐miRNA signature was identified with 234 miRNAs differentially expressed. A subset of these miRNAs was associated with outcome and selected for study in an independent set of 168 cHL samples using quantitative reverse transcription polymerase chain reaction. Multivariate Cox regression analyses including cross‐validation with failure‐free survival (FFS) as clinical endpoint revealed a miRNA signature with MIR21, MIR30E, MIR30D and MIR92B* that identified two risk‐groups with significant differences in 5‐year FFS (81% vs. 35·7%; P < 0·001). Additionally, functional silencing of MIR21 and MIR30D in L428 cells showed increased sensitivity to doxorubicin‐induced apoptosis, pointing towards abnormalities of mitochondrial intrinsic and TP53‐CDKN1A pathways as related to miRNA deregulation in cHL. These results suggest that clinical outcome in cHL is associated with a specific miRNA signature. Moreover, functional analyses suggest a role for MIR21 and MIR30D in cHL pathogenesis and therapeutic resistance.
Effects of perceived behavioral control on the consumer usage intention of e-coupons

To investigate changes that e-coupons bring to consumers' coupon usage, the authors of this article developed and estimated models of coupon-usage intention. The models are based on the theory of reasoned action or the theory of planned behavior. Results show that the theory of planned behavior explains e-coupon usage intention better than the theory of reasoned action. On the other hand, the intention to use traditional coupons is effectively explained by the theory of reasoned action. Both perceived behavioral control and attitude toward Internet searching have significant effects on the intention to use e-coupons. Also, heavy users of e-coupons are different from those of traditional coupons. Light users of traditional coupons have relatively high intention to use e-coupons if they have more access to e-coupons. On the other hand, heavy users of traditional coupons have relatively low intention to use e-coupons if they have less access to them. © 2006 Wiley Periodicals, Inc.
A Ribonuclease Isolated from Wild Ganoderma Lucidum Suppressed Autophagy and Triggered Apoptosis in Colorectal Cancer Cells

The mushroom Ganoderma lucidum (G. lucidum) has been consumed in China as a medicine for promoting health and longevity for thousands of years. Due to its paramount and multiple pharmaceutical effects, G. lucidum has received considerable attention from researchers and its chemical constituents as well as their respective functions were gradually unveiled by using modern research methods. Herein, we reported the isolation of a protein (Ganoderma lucidum ribonuclease, GLR) with anti-colorectal cancer activities from G. lucidum. This protein is a 17.4-kDa RNA degrading enzyme (ribonuclease) and was purified by using liquid chromatography procedures. GLR manifested potent anti-proliferative and anti-colony formation activities on HT29 and HCT116 colorectal cancer cells by inducing cell cycle arrest in G1 phase through the regulation of cyclin D1 and P53 expression. GLR was demonstrated to induce cell apoptosis in HCT116 cells by activating unfolded protein response and caspase-9 regulated pathways. Besides, the ability to undergo autophagy which is a stress adaption mechanism to cope with metabolic crisis was significantly suppressed by GLR treatment in HCT116 cells. The activation of apoptosis in GLR-treated HT29 cells was, however, independent of caspase-9 and the suppression of autophagy was also relatively minor. Thus the apoptosis of HT29 cells triggered by GLR was much milder than that in HCT116 cells. Our findings show that the RNase from G. lucidum may be one of the bioactive components that contribute to the anti-colorectal cancer activity of G. lucidum.
Role of DNA Methylation in Stable Gene Repression*

A large fraction of the animal genome is maintained in a transcriptionally repressed state throughout development. By generating viable Dnmt1-/- mouse cells we have been able to study the effect of DNA methylation on both gene expression and chromatin structure. Our results confirm that the underlying methylation pattern has a profound effect on histone acetylation and is the major effector of me-H3(K4) in the animal genome. We demonstrate that many methylated genes are subject to additional repression mechanisms that also impact on histone acetylation, and the data suggest that late replication timing may play an important role in this process.
THE ROLE OF AFFECT AND LEADERSHIP DURING ORGANIZATIONAL CHANGE

Based on multilevel data collected at 2 points in time, we examine the role of employees’ affective experiences in shaping their commitment and behavioral responses to both the initial (Time 1) and later (Time 2) phases of organizational change (12 months later). We also test the cross-level effect of workgroup managers’ transformational leadership on their employees’ responses to change. We find strong support for predicted longitudinal relationships between employees’ affective experiences and their commitment and behavioral responses to change. In particular, employees’ positive and negative affect (NA) at Time 1 significantly predict both their commitment to change and the 3 dimensions (supportive, resistant, and creative) of behavioral responses at Time 2. Further, the effects of NA directly influence employee change commitment and behaviors at Time 2, whereas the long-term effects of positive affect occur both directly and indirectly through commitment to change at Time 1. Finally, our results support the hypothesized role of workgroup managers’ transformational leadership in shaping employees’ affective reactions and commitment to change at the initial phase of change and thereby, their subsequent behavioral responses in the later phase. We discuss the implications for theory and practice in organizational change.