Miracle-dz/UniFAR-FTUs huggingface dataset:
CD44 standard and variant isoform expression in human epidermal skin tumors is not correlated with tumor aggressiveness but down‐regulated during proliferation and tumor de‐differentiation

CD44 isoforms have been reported to be involved in tumor invasion and metastasis formation. Normal human skin expresses high levels of CD44 isoforms, but little is known about their expression in epidermal skin tumors. Expression of CD44 standard (CD44s) and variant exon (CD44v3, ‐v4, ‐v5, ‐v6, ‐v9)‐encoded gene products has been studied in 74 benign, semi‐malignant and malignant human epithelial skin tumors using a panel of well‐characterized, variant exon‐specific monoclonal antibodies (MAbs). Sensitivity and resolution of the immunohistochemical staining in paraffin sections was substantially improved by using microwave‐based antigen retrieval and an optimized streptavidin‐biotin‐peroxidase technique. Immunostaining was evaluated semi‐quantitatively and correlated with tumor type and degree of histological differentiation by non‐parametric statistical tests. Furthermore, the relationship between CD44 expression and cellular proliferation rate as defined by the Ki‐67 antigen was analyzed in basal cell carcinomas. We found a significant correlation between tumor type and CD44 isoform expression. Basal cell carcinomas exhibited the weakest staining and keratoacanthomas the strongest. Squamous cell carcinomas ranged in between, with a tendency to down‐regulate CD44 expression upon de‐differentiation. In basal cell carcinomas, an inverse relationship between CD44 expression and proliferation rate was directly demonstrated at the cellular level using double immunolabeling. Our data indicate that qualitative and quantitative changes in CD44 splice‐variant expression in human skin tumors do not correlate with invasive and metastatic potential but are rather related to the degree of tumor differentiation. © 1996 Wiley‐Liss, Inc.
The Hungry Stomach: Physiology, Disease, and Drug Development Opportunities

During hunger, a series of high-amplitude contractions of the stomach and small intestine (phase III), which form part of a cycle of quiescence and contractions (known as the migrating motor complex, MMC), play a “housekeeping” role prior to the next meal, and may contribute toward the development of hunger. Several gastrointestinal (GI) hormones are associated with phase III MMC activity, but currently the most prominent is motilin, thought to at least partly mediate phase III contractions of the gastric MMC. Additional GI endocrine and neuronal systems play even more powerful roles in the development of hunger. In particular, the ghrelin-precursor gene is proving to have a complex physiology, giving rise to three different products: ghrelin itself, which is formed from a post-translational modification of des-acyl-ghrelin, and obestatin. The receptors acted on by des-acyl-ghrelin and by obestatin are currently unknown but both these peptides seem able to exert actions which oppose that of ghrelin, either indirectly or directly. An increased understanding of the actions of these peptides is helping to unravel a number of different eating disorders and providing opportunities for the discovery of new drugs to regulate dysfunctional gastric behaviors and appetite. To date, ghrelin and motilin receptor agonists and antagonists have been described. The most advanced are compounds which activate the ghrelin and motilin receptors which are being progressed for disorders associated with gastric hypomotility.
Article in Press G Model the Journal of Systems and Software Using Spin for Automated Debugging of Infinite Executions of Java Programs

This paper presents an approach for the automated debugging of reactive and concurrent Java programs, combining model checking and runtime monitoring. Runtime monitoring is used to transform the Java execution traces into the input for the model checker, the purpose of which is twofold. First, it checks these execution traces against properties written in linear temporal logic (LTL), which represent desirable or undesirable behaviors. Second, it produces several execution traces for a single Java program by generating test inputs and exploring different schedulings in multithreaded programs. As state explosion is the main drawback to model checking, we propose two abstraction approaches to reduce the memory requirements when storing Java states. We also present the formal framework to clarify which kinds of LTL safety and liveness formulas can be correctly analysed with each abstraction for both finite and infinite program executions. A major advantage of our approach comes from the model checker, which stores the trace of each failed execution, allowing the programmer to replay these executions to locate the bugs. Our current implementation, the tool TJT, uses Spin as the model checker and the Java Debug Interface (JDI) for runtime monitoring. TJT is presented as an Eclipse plug-in and it has been successfully applied to debug complex public Java programs.
Increasing Learning and Time Efficiency in Interorganizational New Product Development Teams

Despite the growing popularity of new product development across organizational boundaries, the processes, mechanisms, or dynamics that leverage performance in interorganizational (I-O) product development teams are not well understood. Such teams are staffed with individuals drawn from the partnering firms and are relied on to develop successful new products while at the same time enhancing mutual learning and reducing development time. However, these collaborations can encounter difficulties when partners from different corporate cultures and thought worlds must coordinate and depend on one another and often lead to disappointing performance. To facilitate collaboration, the creation of a safe, supportive, challenging, and engaging environment is particularly important for enabling productive collaborative I-O teamwork and is essential for learning and time efficient product development. This research develops and tests a model of proposed factors to increase both learning and time efficiency on I-O new product teams. It is argued that specific behaviors (caring), beliefs (psychological safety), task-related processes (shared problem solving), and governance mechanisms (clear management direction) create a positive climate that increases learning and time efficiency on I-O teams. Results of an empirical study of 50 collaborative new product development projects indicate that (1) shared problem solving and caring behavior support both learning and time efficiency on I-O teams, (2) team psychological safety is positively related to learning, (3) management direction is positively associated with time efficiency, and (4) shared problem solving is more strongly related to both performance dimensions than are the other factors. The factors supporting time efficiency are slightly different from those that foster learning. The relative importance of these factors also differs considerably for both performance aspects. Overall, this study contributes to a better understanding of the factors that facilitate a favorable environment for productive collaboration on I-O teams, which go beyond contracts or top-management supervision. Establishing such an environment can help to balance management concerns and promote the success of I-O teams. The significance of the results is elevated by the fragility of collaborative ventures and their potential for failure, when firms with different organizational cultures, thought worlds, objectives, and intentions increasingly decide to work across organizational boundaries for the development of new products.
A PROPOSED MECHANISM OF MONENSIN ACTION IN INHIBITING RUMINAL BACTERIAL GROWTH : EFFECTS ON ION FLUX AND PROTONMOTIVE FORCE

Streptococcus bovis, a Gram-positive ruminal bacterium, was unable to grow in the presence of monensin. When monensin (5 mg/liter) was added to actively growing cultures, there was an immediate decrease in growth rate, and within 3 h no further growth was observed. Glucose utilization and lactate production continued for another 8 h even though growth had ceased. Monensin caused a decrease (P<.05) in intracellular K +, a decrease (P<.05) in intracellular pH and an increase (P<.05) in intracellular Na +. The net exchange of K + for Na + and H + via monensin was driven by the difference in concentration of K + and Na + across the cell membrane. Non-treated cells maintained a 70-fold gradient (inside higher) for K +, while the Na + gradient was only 2.7-fold (inside higher). Previous models were based on a reverse mechanism whereby monensin would drive an efflux of Na + from ruminal bacteria, and were not supported by experimental measurements of intracellular Na + and K +, (
Resource Windfalls, Political Regimes, and Political Stability

Abstract We study theoretically and empirically whether natural resource windfalls affect political regimes. We show that windfalls have no effect on democracies, while they have heterogeneous political consequences in autocracies. In deeply entrenched autocracies, the effect of windfalls is virtually nil, while in moderately entrenched autocracies, windfalls significantly exacerbate the autocratic nature of the political system. To frame the empirical work, we present a simple model in which political incumbents choose the degree of political contestability and potential challengers decide whether to try to unseat the incumbents. The model uncovers a mechanism for the asymmetric impact of resource windfalls on democracies and autocracies, as well as the the differential impact within autocracies.
Design and Performance of a Double-Layer Miniaturized-Element Frequency Selective Surface

In this letter, a novel bandpass frequency selective surface (FSS) is proposed. Unlike the traditional FSSs, our building block makes use of a periodic cell with much smaller size than the resonant wavelength. The proposed FSS is composed by two layers of metallic unit cells printed on the substrate. The coupling between parallel patches in different metallic layers can produce the significant distributed capacitance of the FSS; as a result, the size of the FSS unit cell can be decreased to λ/16. Our simulated result shows that the transmission characteristic of the miniaturized FSS is very stable since the relative change rate of resonant frequency is less than 2% with respect to different incident angles from 0° to 45° . The measured results show good agreements with the simulated ones. In addition, our result also implies that the structure has wide application space, based on analysis of three other variations on the periodic structure.
Proteomics and bioinformatics approaches for identification of serum biomarkers to detect breast cancer.

BACKGROUND Surface-enhanced laser desorption/ionization (SELDI) is an affinity-based mass spectrometric method in which proteins of interest are selectively adsorbed to a chemically modified surface on a biochip, whereas impurities are removed by washing with buffer. This technology allows sensitive and high-throughput protein profiling of complex biological specimens. METHODS We screened for potential tumor biomarkers in 169 serum samples, including samples from a cancer group of 103 breast cancer patients at different clinical stages [stage 0 (n = 4), stage I (n = 38), stage II (n = 37), and stage III (n = 24)], from a control group of 41 healthy women, and from 25 patients with benign breast diseases. Diluted serum samples were applied to immobilized metal affinity capture Ciphergen ProteinChip Arrays previously activated with Ni2+. Proteins bound to the chelated metal were analyzed on a ProteinChip Reader Model PBS II. Complex protein profiles of different diagnostic groups were compared and analyzed using the ProPeak software package. RESULTS A panel of three biomarkers was selected based on their collective contribution to the optimal separation between stage 0-I breast cancer patients and noncancer controls. The same separation was observed using independent test data from stage II-III breast cancer patients. Bootstrap cross-validation demonstrated that a sensitivity of 93% for all cancer patients and a specificity of 91% for all controls were achieved by a composite index derived by multivariate logistic regression using the three selected biomarkers. CONCLUSIONS Proteomics approaches such as SELDI mass spectrometry, in conjunction with bioinformatics tools, could greatly facilitate the discovery of new and better biomarkers. The high sensitivity and specificity achieved by the combined use of the selected biomarkers show great potential for the early detection of breast cancer.
The Effect of a High Al Content on the Variation of the Total Oxygen Content in the Steel Melt during a Secondary Refining Process

The aim of this study is to clarify the mechanism of low total oxygen (T.O) contents in high‐Al containing steel grades. Steel samples are taken from a ladle during an LF‐RH process, and the compositions of both the steels and inclusions are determined. According to thermodynamic considerations, the low T.O contents of high Al steel grades are due to the low insoluble oxygen contents. Due to the high Al contents in a steel melt, thermodynamic driving forces of the Al2O3 modification are lower than those in ordinary Al‐killed steels. Both the low thermodynamic driving force of the Al2O3 modification and the inclusion removal from the melts contribute to the low CaO contents in inclusions in high‐Al steel melts. The contact angles of inclusions in high Al steel melts are higher than 90° due to the low CaO content in inclusions. Therefore, the removal tendency of inclusions in high Al steel melts is kept high throughout an LF‐RH process. Due to this high removal tendency, the T.O contents in high Al steel melts decreases remarkably during an LF refining process. Thereafter, they decrease further during the following RH treatment.
Mast cells are critically involved in serum‐mediated vascular leakage in chronic urticaria beyond high‐affinity IgE receptor stimulation

To cite this article: Bossi F, Frossi B, Radillo O, Cugno M, Tedeschi A, Riboldi P, Asero R, Tedesco F, Pucillo C. Mast cells are critically involved in serum‐mediated vascular leakage in chronic urticaria beyond high‐affinity IgE receptor stimulation. Allergy 2011; 66: 1538–1545.
Self-employment of immigrants and natives in Sweden – a multilevel analysis

Recent research suggests that self-employment among immigrants is due to a combination of multiple situational, cultural and institutional factors, all acting together. Using multilevel regression and unique data on the entire population of Sweden for the year 2007, this study attempts to quantify the relative importance for the self-employed of embeddedness in ethnic contexts (country of birth) and regional business and public regulatory frameworks (labour market areas). This information indicates whether the layers under consideration are valid constructs of the surroundings that influence individual self-employment. The results show that 10% (women) and 8% (men) of the total variation in individual differences in self-employment can be attributed to the country of birth. When labour market areas are included in the analyses, the share of the total variation increases to 14% for women and 12% for men. The results show that the ethnic context and the economic environment play a minor role in understanding individual differences in self-employment levels. The results can have important implications when planning interventions or other actions focusing on self-employment as public measures to promote self-employment often are based on geographic areas and ethnic contexts.
An Electronic Publication Dedicated to Early Stellar Evolution and Molecular Clouds Abstracts of Recently Accepted Papers the H Ii Region/pdr Connection: Self-consistent Calculations of Physical Conditions in Star-forming Regions the Onset of Planet Formation in Brown Dwarf Disks

We have performed a series of calculations designed to reproduce infrared diagnostics used to determine physical conditions in star-forming regions. We self-consistently calculate the thermal and chemical structure of an H II region and photodissociation region (PDR) that are in pressure equilibrium. This differs from previous work, which used separate calculations for each gas phase. Our calculations span a wide range of stellar temperatures, gas densities, and ionization parameters. We describe improvements made to the spectral synthesis code Cloudy that made these calculations possible. These include the addition of a molecular network with ∼1000 reactions involving 68 molecular species and improved treatment of the grain physics. Data from the Spitzer First Look Survey, along with other archives, are used to derive important physical characteristics of the H II region and PDR. These include stellar temperatures, electron densities, ionization parameters, UV radiation flux (G 0), and PDR density. Finally, we calculate the contribution of the H II region to PDR emission line diagnostics, which allows for a more accurate determination of physical conditions in the PDR. The onset of planet formation in protoplanetary disks is marked by the growth and crystallization of sub-micrometer-sized dust grains accompanied by dust settling toward the disk mid-plane. Here, we present infrared spectra of disks around brown dwarfs and brown dwarf candidates. We show that all three processes occur in such cool disks in a way similar or identical to that in disks around low-and intermediate-mass stars. These results indicate that the onset of planet formation extends to disks around brown dwarfs, suggesting that planet formation is a robust process occurring in most young circumstellar disks.
Biological and Biomaterial Approaches for Improved Islet Transplantation

Islet transplantation may be used to treat type I diabetes. Despite tremendous progress in islet isolation, culture, and preservation, the clinical use of this modality of treatment is limited due to post-transplantation challenges to the islets such as the failure to revascularize and immune destruction of the islet graft. In addition, the need for lifelong strong immunosuppressing agents restricts the use of this option to a limited subset of patients, which is further restricted by the unmet need for large numbers of islets. Inadequate islet supply issues are being addressed by regeneration therapy and xenotransplantation. Various strategies are being tried to prevent β-cell death, including immunoisolation using semipermeable biocompatible polymeric capsules and induction of immune tolerance. Genetic modification of islets promises to complement all these strategies toward the success of islet transplantation. Furthermore, synergistic application of more than one strategy is required for improving the success of islet transplantation. This review will critically address various insights developed in each individual strategy and for multipronged approaches, which will be helpful in achieving better outcomes.
Anxiety within families: interrelations, consistency, and change.

In a community sample of mothers (N = 763), each with a focal child aged 4.5 years, anxiety levels were high. Only 54% of mothers had anxiety scores within the "normal" Hospital Anxiety and Depression Scale (HADS) range, compared with 85% for depression. A subsample (N = 116) was selected for two-parent families, one to three children, and mothers spread over low, medium, and high anxiety levels. Mothers' anxiety was not significantly related to age, education, or work status, but rather to mothers' and fathers' independent ratings of marital satisfaction and family functioning, and to fathers' own anxiety and depression. Fathers' anxiety was related not to their own views of marital satisfaction and family functioning, but rather to mothers' views and to maternal anxiety. Assessments 8 years later--of anxiety, depression, and family functioning--showed high consistency over time, particularly maternal anxiety (r = .70) and paternal depression (r = .81). Although means did not change significantly over time for fathers, mothers' anxiety, depression, and perceptions of family functioning all improved (p < .001). For parents who were later to separate (compared with the others), initial family functioning, dyadic adjustment, and maternal anxiety were significantly "worse. " The strongest predictor of later break-up was fathers' dyadic adjustment.
A second Epstein-Barr virus membrane protein (LMP2) is expressed in latent infection and colocalizes with LMP1

Recent cDNA cloning and sequencing of two Epstein-Barr virus (EBV)-specific mRNAs from latently infected cultures revealed that these RNAs are encoded across the fused terminal repeats of the viral genome and that they are likely to encode two nearly identical proteins with the same transmembrane domains. The smaller predicted protein (LMP2B) lacks 119 amino-terminal amino acids found in the larger one (LMP2A). To test whether these proteins are expressed in latently infected lymphocytes, antibodies to the LMP2 proteins were derived by immunizing rabbits with TrpE-LMP2A fusion proteins. Affinity-purified LMP2-specific antibodies recognized 54- and 40-kilodalton proteins, corresponding to LMP2A and LMP2B, in immunoblots of rodent fibroblasts stably transfected with eucaryotic expression plasmids containing either the LMP2A or LMP2B cDNA. Similar-size proteins were also identified in immunoblots of latently infected lymphocytes. LMP2A localized to membranes in cellular fractionation studies. In immunofluorescent studies, LMP2 localized in the plasma membrane of EBV-infected lymphocytes, with the majority of reactivity confined to the region of the LMP1 patch. This reactivity was detected in almost all lymphoblastoid cells latently infected with EBV.
Individual Attitudes Towards Skilled Migration: An Empirical Analysis Across Countries

It is commonly argued that skilled immigration benefits the destination country through several channels. Yet, only a small group of countries reports to have policies in place aimed at increasing the intake of skilled immigrants. Why? In this paper we analyze the factors that affect a direct measure of individual attitudes towards skilled migration, focusing on two main channels: the labor market and the welfare state. We find that more educated natives are less likely to favor skilled immigration - consistent with the labor-market channel - while richer people are more likely to do so - in accordance with the welfare state channel under the tax adjustment model. Our findings thus suggest that the labor market competition threat perceived by skilled natives in the host countries might be driving the observed cautious policies.
High prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae infections in anal and pharyngeal sites among a community-based sample of men who have sex with men and transgender women in Lima, Peru

Objectives This study aimed to characterise the epidemiology of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections among men who have sex with men (MSM) and transgender women (TW) in Lima, Peru. Setting Cross-sectional study in Lima, Peru. Participants We recruited a group of 510 MSM and 208 TW for a subsequent community-based randomised controlled trial. The presence of CT and NG were evaluated using Aptima Combo2 in pharyngeal and anal swabs. We also explored correlates of these infections. Primary and secondary outcome measures: Study end points included overall prevalence of C. trachomatis and N. gonorrhoeae in anal and pharyngeal sites. Results Overall prevalence of CT was 19% (95% CI 16.1% to 22.1%) and 4.8% (95% CI 3.3% to 6.6%) in anal and pharyngeal sites, respectively, while prevalence of NG was 9.6% (95% CI 7.5% to 12.0%) and 6.5% (95% CI 4.8% to 8.5%) in anal and pharyngeal sites, respectively. Conclusions The prevalence of each infection declined significantly among participants older than 34 years (p<0.05). Efforts towards prevention and treatment of extraurogenital chlamydial and gonococcal infections in high-risk populations like MSM and TW in Lima, Peru, are warranted. Trial registration number NCT00670163; Results.