Miracle-dz/UniFAR-FTUs huggingface dataset:
A dominant function of p38 mitogen-activated protein kinase signaling in receptor activator of nuclear factor-kappaB ligand expression and osteoclastogenesis induction by Aggregatibacter actinomycetemcomitans and Escherichia coli lipopolysaccharide.

BACKGROUND AND OBJECTIVE Lipopolysaccharide from gram-negative bacteria is one of the microbial-associated molecular patterns that initiate the immune/inflammatory response, leading to the tissue destruction observed in periodontitis. The aim of this study was to evaluate the role of the p38 mitogen-activated protein kinase (MAPK) signaling pathway in lipopolysaccharide-induced receptor activator of nuclear factor-kappaB ligand (RANKL) expression by murine periodontal ligament cells. MATERIAL AND METHODS Expression of RANKL and osteoprotegerin mRNA was studied by reverse transcription-polymerase chain reaction upon stimulation with lipopolysaccharide from Escherichia coli and Aggregatibacter actinomycetemcomitans. The biochemical inhibitor SB203580 was used to evaluate the contribution of the p38 MAPK signaling pathway to lipopolysaccharide-induced RANKL and osteoprotegerin expression. Stable cell lines expressing dominant-negative forms of MAPK kinase (MKK)-3 and MKK6 were generated to confirm the role of the p38 MAPK pathway. An osteoclastogenesis assay using a coculture model of the murine monocytic cell line RAW 264.7 was used to determine if osteoclast differentiation induced by lipopolysaccharide-stimulated periodontal ligament was correlated with RANKL expression. RESULTS Inhibiting p38 MAPK prior to lipopolysaccharide stimulation resulted in a significant decrease of RANKL mRNA expression. Osteoprotegerin mRNA expression was not affected by lipopolysaccharide or p38 MAPK. Lipopolysaccharide-stimulated periodontal ligament cells increased osteoclast differentiation, an effect that was completely blocked by osteoprotegerin and significantly decreased by inhibition of MKK3 and MKK6, upstream activators of p38 MAPK. Conditioned medium from murine periodontal ligament cultures did not increase osteoclast differentiation, indicating that periodontal ligament cells produced membrane-bound RANKL. CONCLUSION Lipopolysaccharide resulted in a significant increase of RANKL in periodontal ligament cells. The p38 MAPK pathway is required for lipopolysaccharide-induced membrane-bound RANKL expression in these cells.
A Lightweight and Efficient Tool for Cleaning Web Pages

Originally conceived as a naïve baseline experiment using traditional n-gram language models as classifiers, the NCleaner system has turned out to be a fast and lightweight tool for cleaning Web pages with state-of-the-art accuracy (based on results from the CLEANEVAL competition held in 2007). Despite its simplicity, the algorithm achieves a significant improvement over the baseline (i.e. plain, uncleaned text dumps), trading off recall for substantially higher precision. NCleaner is available as an open-source software package. It is pre-configured for English Web pages, but can be adapted to other languages with minimal amounts of manually cleaned training data. Since NCleaner does not make use of HTML structure, it can also be applied to existing Web corpora that are only available in plain text format, with a minor loss in classfication accuracy.
An empirical investigation into the determinants of life satisfaction in New Zealand

The past decade has seen an increasing body of evidence emerge that subjective wellbeing can be empirically measured through relatively straightforward questions on self-reported happiness or life satisfaction carried in sample surveys. This paper analyses the determinants of subjective wellbeing using data from the 2008 New Zealand General Social Survey. The paper first provides a brief summary of what is known about the determinants of subjective wellbeing from the international literature. A simple model of the determinants of subjective wellbeing in New Zealand is described, and coefficients for the model are estimated using ordinary least squares. The model is re-estimated using an ordered Probit to confirm that the results are not biased due to the ordinal nature of the data. The paper finds that demographic factors are largely not significant drivers of wellbeing in New Zealand and conforms to the international literature in identifying income, unemployment, health status, and social contact as the four main factors affecting subjective wellbeing.
What clears burglary offenses? Estimating the influences of multiple perspectives of burglary clearance in Philadelphia

Purpose – Although many studies have examined the correlates of homicide clearance rates, few analyses have examined the factors related to the clearance of burglary offenses. The purpose of this paper is to address several gaps in the literature to determine if burglary clearance rates are due to discretionary, non-discretionary, and/or neighborhood contextual factors. Design/methodology/approach – Data are analyzed from more than 10,000 burglary incidents in Philadelphia from 2010 using multilevel models to simultaneously test for the influence of multiple perspectives of the factors of crime clearance. Findings – The results indicate that variables representing broken windows enforcement, discretionary factors, and non-discretionary factors are related to the increased likelihood that burglaries are cleared, but processes associated with social disorganization within communities is not. Research limitations/implications – The findings contribute to the literature by showing that future examinations of .
Anger, Fear, or Sadness: Faculty Members' Emotional Reactions to Collective Pay Disadvantage

As part of a mail survey about their work experiences, university faculty members reported their specific emotional reactions to group inequities in faculty pay and benefits. The results indicate that sadness, fear; and anger are distinct emotional responses to a collective disadvantage. Group-based anger mediated the relationship between collective disadvan tage and willingness to protest whereas group-based sadness mediated the relationship between collective disadvantage and organizational loyalty. Based on an integration of cognitive appraisal models of emotion with RD theory, four other predictors of intergroup emotions-(]) the legitimacy of the process that produced the deprivation, (2) whether another agent was responsible, (3) group efficacy, and (4) whether the situation would improve or become worse-were identified and tested. The measurement of specific emo tional reactions to perceived collective disadvantage extends and refines RD approaches to collective action and organizational loyalty.
Evaluation of the Titertek-NF system for identification of gram-negative nonfermentative and oxidase-positive fermentative bacteria

The Titertek-NF (TT-NF) system (Flow Laboratories GmbH, Meckenheim, Federal Republic of Germany) was evaluated for the identification of 1,289 strains of gram-negative, nonfermentative bacteria and some gram-negative, oxidase-positive bacteria. The oxidase test was also performed. Identifications were classified as correct, not identified (two or more taxa possible and identification score of less than 80%; supplementary tests for furthering the identification were not performed), and incorrect. Correct identification results were further subdivided by the correct level of species or biotype identification as greater than or equal to 98% (category a), 90 to 97% (category b), and 80 to 89% (category c). When compared with conventional identification results, the TT-NF system correctly identified 90.3% of strains (1,164 of 1,289 strains), with 72.5% (935 strains) belonging to category a, 14.7% of strains (189 strains) belonging to category b, and 3.1% of strains (40 strains) belonging to category c. Among the remaining strains, 104 (8.1%) were not identified and 14 (1.1%) were misidentified, and the system failed to generate identification results for 7 strains (0.5%). Reactions within the TT-NF system were reproducible, with an estimated probability of erroneous test results of 0.2%.
The Career Transitions Inventory: Measuring Internal Resources in Adulthood

The purpose of this article is to describe the development and application of the Career Transition Inventory (CTI; Heppner, 1991). The CTI is designed to assess psychological resources, or conversely barriers, that adults may experience during a career transition. This article discusses the CTI scale construction, including its theoretical base, factor analysis, reliability, and validity estimates. In addition, information is provided on how to interpret the various scales to adults in career transition.
Integrin-associated Protein Is a Receptor for the C-terminal Domain of Thrombospondin (*)

The C-terminal “cell-binding domain” (CBD) of thrombospondin-1 (TS1) is a binding site for many cell types. Cell-binding peptides based on the sequence RFYVVM from the CBD of TS1 affinity label a 52-kDa cell surface glycoprotein, which we show is integrin-associated protein (IAP or CD47). IAP associates with αβ and thereby modulates the activity of several integrins. Cells that express IAP bind strongly to TS1, the CBD, and its active cell-binding peptides while IAP negative cells do not. The 52-kDa protein is affinity labeled on IAP-positive but not IAP-negative cells, and monoclonal antibodies against IAP specifically immunoprecipitate the affinity-labeled 52-kDa protein from lysates of IAP-positive cells. Consistent with the association of IAP with αβ integrin, the labeled 52-kDa protein is immunoprecipitated by an anti-αβ antibody. Endothelial cells exhibit chemotaxis toward TS1 (at concentrations above 10 nM) and RFYVVM peptides. Chemotaxis to both agents is specifically inhibited by a function blocking anti-IAP monoclonal antibody. These data establish IAP (CD47) as a receptor for the CBD of TS1 and suggest a mechanism for the well established effects of the CBD on cell motility.
Whole transcriptome organisation in the dehydrated supraoptic nucleus

The supraoptic nucleus (SON) is part of the central osmotic circuitry that synthesises the hormone vasopressin (Avp) and transports it to terminals in the posterior lobe of the pituitary. Following osmotic stress such as dehydration, this tissue undergoes morphological, electrical and transcriptional changes to facilitate the appropriate regulation and release of Avp into the circulation where it conserves water at the level of the kidney. Here, the organisation of the whole transcriptome following dehydration is modelled to fit Zipf's law, a natural power law that holds true for all natural languages, that states if the frequency of word usage is plotted against its rank, then the log linear regression of this is -1. We have applied this model to our previously published euhydrated and dehydrated SON data to observe this trend and how it changes following dehydration. In accordance with other studies, our whole transcriptome data fit well with this model in the euhydrated SON microarrays, but interestingly, fit better in the dehydrated arrays. This trend was observed in a subset of differentially regulated genes and also following network reconstruction using a third-party database that mines public data. We make use of language as a metaphor that helps us philosophise about the role of the whole transcriptome in providing a suitable environment for the delivery of Avp following a survival threat like dehydration.
Quantitative Detection of Active Vibrios Associated with White Plague Disease in Mussismilia braziliensis Corals

Over recent decades several coral diseases have been reported as a significant threat to coral reef ecosystems causing the decline of corals cover and diversity around the world. The development of techniques that improve the ability to detect and quantify microbial agents involved in coral disease will aid in the elucidation of disease cause, facilitating coral disease detection and diagnosis, identification and pathogen monitoring, pathogen sources, vectors, and reservoirs. The genus Vibrio is known to harbor pathogenic strains to marine organisms. One of the best-characterized coral pathogens is Vibrio coralliilyticus, an aetilogic agent of White Plague Disease (WPD). We used Mussismilia coral tissue (healthy and diseased specimens) to develop a rapid reproducible detection system for vibrios based on RT-QPCR and SYBR chemistry. We were able to detect total vibrios in expressed RNA targeting the 16S rRNA gene at 5.23 × 106 copies/μg RNA and V. coralliilyticus targeting the pyrH gene at 5.10 × 103 copies/μg RNA in coral tissue. Detection of V. coralliilyticus in diseased and in healthy samples suggests that WPD in the Abrolhos Bank may be caused by a consortium of microorganism and not only a single pathogen. We developed a more practical and economic system compared with probe uses for the real-time detection and quantification of vibrios from coral tissues by using the 16S rRNA and pyrH gene. This qPCR assay is a reliable tool for the monitoring of coral pathogens, and can be useful to prevent, control, or reduce impacts in this ecosystem.
Cardiovascular and affective responses to social stress in adolescents with internalizing and externalizing problems

Behavioral responses to stress and challenge are based in emotional and physiological arousal reactions. Adolescents with maladaptive or problematic behavior patterns, such as internalizing or externalizing problems, are likely to show atypical emotional and physiological reactions to stress. Relations between problems and reactions to stress were examined in a sample of 55 young adolescents with normal to clinical levels of internalizing and externalizing problems. Youth had their self-reported emotional states, heart rate, and blood pressure levels measured before and after engaging in two socially challenging, evaluative manipulations. Internalizing problems were associated with less positive affect but greater anxiety and cardiovascular arousal, whereas externalizing problems predicted greater hostility and positive affect but less cardiovascular arousal. The necessity of recognizing and incorporating comorbid characteristics and multiple response systems into studies of the links between problems and reactivity is emphasized in the discussion.
Low level Visual Processing Skills of Adults and Children with Dyslexia

Previous work by Lovegrove and colleagues Lovegrove Martin Slaghuis 1986 suggested that dyslexic children show visual deficits specific to transient processing We designed and examined a number of threshold and suprathreshold tasks to test the generality of their claims We first compared the performance of adult dyslexics and child dyslexics to the performance of age matched normal readers on a series of threshold flicker tasks In contrast to the earlier results dyslexics and normal readers did not differ in their contrast thresholds for flickering sinewave gratings Dyslexic children and normal readers also showed similar performance on two suprathreshold visual search tasks that evaluated transient processing The evidence suggests that a transient processing deficit is not a general characteristic of developmental dyslexia Claims that visual factors play a role in dyslexia must address the confounding role of performance and attentional factors
Bovine Staphylococcus aureus Secretes the Leukocidin LukMF′ To Kill Migrating Neutrophils through CCR1

ABSTRACT Although Staphylococcus aureus is best known for infecting humans, bovine-specific strains are a major cause of mastitis in dairy cattle. The bicomponent leukocidin LukMF′, exclusively harbored by S. aureus of ruminant origin, is a virulence factor associated with bovine infections. In this study, the molecular basis of the host specificity of LukMF′ is elucidated by identification of chemokine receptor CCR1 as its target. Bovine neutrophils, the major effector cells in the defense against staphylococci, express significant cell surface levels of CCR1, whereas human neutrophils do not. This causes the particular susceptibility of bovine neutrophils to pore formation induced by LukMF′. Bovine S. aureus strains produce high levels of LukMF′ in vitro. In culture supernatant of the mastitis field isolate S1444, LukMF′ was the most important cytotoxic agent for bovine neutrophils. In a fibrin gel matrix, the effects of the in situ secreted toxins on neutrophils migrating toward S. aureus were visualized. Under these physiological ex vivo conditions, bovine S. aureus S1444 efficiently killed approaching neutrophils at a distance through secretion of LukMF′. Altogether, our findings illustrate the coevolution of pathogen and host, provide new targets for therapeutic and vaccine approaches to treat staphylococcal diseases in the cow, and emphasize the importance of staphylococcal toxins in general. IMPORTANCE This study explains the mechanism of action of LukMF′, a bicomponent toxin found in bovine lineages of S. aureus that is associated with mastitis in cattle. At a molecular level, we describe how LukMF′ can specifically kill bovine neutrophils. Here, we demonstrate the contribution of toxins in the determination of host specificity and contribute to the understanding of mechanisms of coevolution of pathogen and host. Our study provides new targets that can be used in therapeutic and vaccine approaches to treat staphylococcal diseases in the cow. We also demonstrate the importance of toxins in specific elimination of immune cells, which has broader implications, especially in human infections. This study explains the mechanism of action of LukMF′, a bicomponent toxin found in bovine lineages of S. aureus that is associated with mastitis in cattle. At a molecular level, we describe how LukMF′ can specifically kill bovine neutrophils. Here, we demonstrate the contribution of toxins in the determination of host specificity and contribute to the understanding of mechanisms of coevolution of pathogen and host. Our study provides new targets that can be used in therapeutic and vaccine approaches to treat staphylococcal diseases in the cow. We also demonstrate the importance of toxins in specific elimination of immune cells, which has broader implications, especially in human infections.
Spontaneity of augmentative and alternative communication in persons with intellectual disabilities: critical review

This paper examines the issue of communicative spontaneity in natural environments of persons with intellectual disabilities who rely on augmentative or alternative communication (AAC) systems. A number of data-based studies are examined and their findings discussed. Unfortunately, interpretation of existing research is complicated by a number of factors including outdated conceptualizations and definitions of spontaneity. It is argued that future research needs to adopt a more sophisticated model of spontaneity and systematically investigate a broad range of environmental variables that may occasion communication in persons with severe disabilities. Such fundamental research may well provide a foundation for the development of more effective strategies to enhance spontaneity.
Maternal Diabetic Control and Hypertrophic Cardiomyopathy in Infants of Diabetic Mothers

Hypertrophic cardiomyopathy has been well documented in infants of diabetic mothers (IDMs). If this asymmetric septal enlargement is an anabolic result of fetal hyperinsulinemia triggered by maternal hyperglycemia during the third trimester, maternal glycosylated hemoglobin (HbA1) levels, an indicator of glycemic control, should then correlate positively at delivery with newborn ventricular septal thickness. In this study of 20 infants of well-controlled diabetic mothers, no relationship was observed between echocardiographic evidence of hypertrophic cardiomyopathy and maternal HbA1 levels. Seven babies (35%) exhibited exaggerated septal thickening, but none had cardiac-specific symptoms. Although 60 percent of the IDMs were large for gestational age and 45 percent demonstrated neonatal hypoglycemia, neither of these complications correlated with maternal HbA 1. In this group of babies of well-controlled diabetic women, echocardiographic indicators of cardiomyopathy were common, but clinical evidence of cardiac embarrassment was not observed. Moreover, these data do not support third trimester maternal hyperglycemia as instrumental in the etiology of cardiomyopathy and other complications of IDMs.
Lipid Peroxidation and Nitric Oxide Levels in Male Smokers' Spermatozoa and their Relation with Sperm Motility

Background Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as nitric oxide synthases. Low concentrations of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. Moreover, sperm membrane contains high concentrations of polyunsaturated fatty acids that are highly susceptible to oxidative damage that interferes with fertilization ability. Therefore, we investigated the correlation between levels of sperm malondialdehyde (MDA) and NO with sperm motility in male smokers. Methods Semen samples were collected from normozoospermic smoker (n=64) and nonsmoker (n=83) men. The content of sperm lipid peroxidation was determined by measuring malondialdehyde (MDA). The sperm NO were also measured using Griess reagent. Data was analyzed by SPSS, (version 15.0), using independent t-test and Pearson analysis. Results The mean MDA and NO concentrations in the sperm of normozoospermic male smokers were significantly higher than the control group or normozoospermic nonsmokers, (p <0.001). A significant negative relationship was noted between sperm motility and sperm MDA levels (r=−0.32, p=0.01); and sperm motility and sperm NO concentration (for nitrite, r=−0.34, p=0.006 and for nitrate, r=−0.38, p=0.002). Conclusion It was concluded that the increase in MDA and NO production in sperm can influence sperm motility in normozoospermic smokers. Therefore, it seems that cigarette smoking may affect the fertility of male smokers via increasing the amount of sperm MDA/lipid peroxidation and NO concentrations.
One Hundred Questions of Importance to the Conservation of Global Biological Diversity

Abstract: We identified 100 scientific questions that, if answered, would have the greatest impact on conservation practice and policy. Representatives from 21 international organizations, regional sections and working groups of the Society for Conservation Biology, and 12 academics, from all continents except Antarctica, compiled 2291 questions of relevance to conservation of biological diversity worldwide. The questions were gathered from 761 individuals through workshops, email requests, and discussions. Voting by email to short‐list questions, followed by a 2‐day workshop, was used to derive the final list of 100 questions. Most of the final questions were derived through a process of modification and combination as the workshop progressed. The questions are divided into 12 sections: ecosystem functions and services, climate change, technological change, protected areas, ecosystem management and restoration, terrestrial ecosystems, marine ecosystems, freshwater ecosystems, species management, organizational systems and processes, societal context and change, and impacts of conservation interventions. We anticipate that these questions will help identify new directions for researchers and assist funders in directing funds.