Miracle-dz/UniFAR-FTUs huggingface dataset:
Carbapenem Heteroresistance in VIM-1-Producing Klebsiella pneumoniae Isolates Belonging to the Same Clone: Consequences for Routine Susceptibility Testing

ABSTRACT Susceptibility results with low reproducibility by the same or different methods have been observed for metallo-beta-lactamase (MBL)-producing Enterobacteriaceae. Eighteen VIM-1-producing Klebsiella pneumoniae isolates (one per patient) belonging to a single epidemic clone in our hospital (2005 to 2008) but with different susceptibilities to carbapenems were studied. Imipenem MICs ranged from 8 to >128 mg/liter by standard CLSI microdilution, from ≤1 to >8 mg/liter by the semiautomatic Wider system, and from 0.75 to >32 mg/liter by Etest. Meropenem MICs ranged from 0.5 to 128, ≤1 to >8, and 0.38 to >32 mg/liter, respectively. Ertapenem MICs by CLSI microdilution and Etest ranged from 1 to 64 and 0.75 to >32 mg/liter, respectively. The rates of essential agreement (±1 log2 dilution) for imipenem and meropenem MICs between the Wider system and the reference microdilution method were 45% and 49%, respectively. Those between Etest and the reference microdilution method for imipenem, meropenem, and ertapenem MICs were 33%, 67%, and 84%. The rates of very major errors for the Wider system and Etest were 33% and 28% for imipenem and 25% and 75% for meropenem, respectively. Low MIC reproducibility was observed even when the same inoculum was used (differences up to 4-fold dilutions). Heteroresistance was suspected due to the presence of colonies in the Etest inhibition zone. It was confirmed by population analysis profiles of 4 isolates displaying different imipenem MICs, with the exception of an OmpK36-porin-deficient isolate that homogeneously expressed carbapenem resistance (MIC, >128 mg/liter). Low carbapenem MIC reproducibility could be due to the presence of resistant subpopulations and variable expression of the resistance mechanisms. Since carbapenem MICs are not good markers of MBL production, reliable and reproducible phenotypic methods are needed to detect the presence of this mechanism.
Initial state of the Drosophila eye before dorsoventral specification is equivalent to ventral

Dorsoventral (DV) patterning is crucial for eye development in invertebrates and higher animals. DV lineage restriction is the primary event in undifferentiated early eye primordia of Drosophila. In Drosophila eye disc, a dorsal-specific GATA family transcription factor pannier (pnr) controls Iroquois-Complex (Iro-C) genes to establish the dorsal eye fate whereas Lobe (L), which is involved in controlling a Notch ligand Serrate (Ser), is specifically required for ventral growth. However, fate of eye disc cells before the onset of dorsal expression of pnr and Iro-C is not known. We show that L/Ser are expressed in entire early eye disc before the expression of pnr and Iro-C is initiated in late first instar dorsal eye margin cells. Our evidence suggests that during embryogenesis pnr activity is not essential for eye development. We present evidence that loss of L or Ser function prior to initiation of pnr expression results in elimination of the entire eye, whereas after the onset of pnr expression it results only in preferential loss of ventral half of eye. We demonstrate that dorsal eye disc cells also become L or Ser dependent when they are ventralized by removal of pnr or Iro-C gene function. Therefore, we propose that early state of the eye prior to DV lineage restriction is equivalent to ventral and requires L and Ser gene function.
Sexual dimorphism in 11 β hydroxysteroid dehydrogenase activity and its relation to fat distribution and insulin sensitivity; a study in hypopituitary subjects

Sexual dimorphism of 11 β hydroxysteroid dehydrogenase activity (11 β HSD) as measured by the urinary 11‐OH/11‐oxo cortisol metabolite ratio has been documented in healthy subjects. Since body composition, fat distribution and insulin sensitivity vary between the sexes we have investigated whether these factors may account for the observed difference. Studies were performed in ACTH deficient hypopituitary subjects to eliminate the effect of feedback modulation of cortisol secretion.
Determinants of Appropriate Child Health and Nutrition Practices among Women in Rural Gambia

Health education and awareness involves providing knowledge about causes of illness and choices to promote a change in individual behaviour and, thus, improves survival of individuals. Studies have, however, shown that improved knowledge and awareness is not always translated into appropriate actions. This study aimed at exploring the factors determining mothers’ choices of appropriate child health and nutrition practices in the Gambia. Eight focus-group discussions (FGDs) were held with 63 women whose children had been seen at the Keneba MRC Clinic within the 12 months preceding the study. The FGDs were analyzed using a thematic framework. Gender inequality, presence or absence of support networks, alternative explanatory models of malnutrition, and poverty were identified as the main factors that would determine the ability of a mother to practise what she knows about child health and nutrition. The findings highlight the need to consider the broader social, cultural and economic factors, including the value of involving men in childcare, when designing nutritional interventions.
Blink detection for real-time eye tracking

This work is motivated by our goal of providing non-contact head and eye based control of computer systems for people with motor difficulties. The system described here uses spatio-temporal filtering and variance maps to locate the head and find the eye-feature points, respectively. These feature points are accurately tracked in the succeeding frames by using a modified version of the Lucas? Kanade tracking algorithm with pyramidal implementation. Accurate head and eye tracking results are obtained at a processing rate of more than 30 frames per second (fps) in more than 90% cases with a low false positive blink detection rate of 0.01%. This is achieved under varying lighting conditions for people of different ethnicity, with and without wearing glasses.
Actin‐based motility of Burkholderia pseudomallei involves the Arp 2/3 complex, but not N‐WASP and Ena/VASP proteins

The facultative intracellular bacterium Burkholderia pseudomallei induces actin rearrangement within infected host cells leading to formation of actin tails and membrane protrusions. To investigate the underlying mechanism we analysed the contribution of cytoskeletal proteins to B. pseudomallei‐induced actin tail assembly. By using green fluorescent protein (GFP)‐fusion constructs, the recruitment of the Arp2/3 complex, vasodilator‐stimulated phosphoprotein (VASP), Neural Wiskott–Aldrich syndrome protein (N‐WASP), zyxin, vinculin, paxillin and α‐actinin to the surface of B. pseudomallei and into corresponding actin tails was studied. In addition, antibodies against the same panel of proteins were used for immunolocalization. Whereas the Arp2/3 complex and α‐actinin were incorporated into B. pseudomallei‐induced actin tails, none of the other proteins were detected in these structures. The overexpression of an Arp2/3 binding fragment of the Scar1 protein, shown previously to block actin‐based motility of Listeria, had no effect on B. pseudomallei tail formation. Infections of either N‐WASP‐ or Ena/VASP‐defective cells showed that these proteins are not essential for B. pseudomallei‐induced actin polymerization. In conclusion, our results suggest that B. pseudomallei induces actin polymerization through a mechanism that differs from those evolved by Listeria, Shigella, Rickettsia or vaccinia virus.
Inhibition of Mitogenesis in Balb/c-3T3 Cells by Trichostatin A

Trichostatin A (TSA), a global repressor of histone deacetylase activity, inhibits the proliferation of a number of cell types. However, the identification of the mechanisms underlying TSA-mediated growth arrests has remained elusive. In order to resolve in more detail the cellular process modulated during the growth inhibition induced by TSA, we studied the effect of the drug on G0/G1 traverse in mitogen-stimulated quiescent Balb/c-3T3 cells. Cyclin D1 and retinoblastoma proteins were induced following the mitogenic stimulation of both control and TSA-treated cells, and cyclin D1 formed complexes with CDK4 under both conditions. However, cyclin D1-associated kinase was not increased in growth-arrested cells. The lack of cyclin D-associated kinase was paralleled by an accumulation of RB in a hypophosphorylated form, as would be expected. In contrast, p130 became partially phosphorylated, accompanied by a marked increase in p130-dependent E2F DNA binding activity and a partial release of free E2F-4. Despite the presence of E2F complexes not bound to pocket proteins, late G1 E2F-dependent gene expression was not observed. The lack of cyclin D1-associated kinase in TSA-treated cultures was potentially due to high levels of the cyclin-dependent inhibitor p27 kip1 . However, the modulation of p27 kip1 levels by the deacetylase inhibitor cannot be responsible for the induction of the cell cycle arrest, since the growth of murine embryo fibroblasts deficient in both p27 kip1 and p21 cip1 was also inhibited by TSA. These data support a model in which TSA inhibits very early cell cycle traverse, which, in turn, leads to a decrease in cyclin D1-associated kinase activation and a repression of late cell cycle-dependent events. Alterations in early G0/G1 gene expression accompany the TSA-mediated growth arrest.
Arginine Decarboxylase and Putrescine Oxidase in Ovaries of Pisum sativum L. (Changes during Ovary Senescence and Early Stages of Fruit Development)

Enzymatic activities involved in putrescine metabolism in ovaries of Pisum sativum L. during ovary senescence and fruit set were investigated. Accumulation of putrescine was observed during incubation of extracts from gibberellic acid-treated unpollinated ovaries (young developing fruits) but not in extracts from untreated ovaries (senescent ovaries). Extracts from pea ovaries showed arginine decarboxylase (ADC) activity, but ornithine decarboxylase and arginase activity were not detected. ADC activity decreased in presenescent ovaries and increased markedly after induction of fruit set with gibberellic acid. Increases in ADC activity were also observed with application of other plant growth substances (benzy-ladenine and 2,4-dichlorophenoxyacetic acid), after pollination, and in the slender (la crys) pea mutant. By contrast, putrescine oxidase activity increased in presenescent ovaries but did not increase during early fruit development. All of these results suggest that ADC and putrescine oxidase are involved in the control of putrescine metabolism. Ovary senescence is characterized by the absence of putrescine biosynthesis enzymes and increased levels of putrescine oxidase and fruit development by an increase in ADC and a constant level of putrescine oxidase.
Modifying Anti-Bullying Programs to Include Students with Disabilities

Bullying is identified as one of the most predominant problems faced by children in the United States education system . 'J as well as one of the most significant health risks to children. Most of us have seen or experienced bullying at some point in our schooling and we know that some students are more at risk of being targeted. Bullying is defined as any aggressive behavior with the intent to harm that involves a real or perceived power imbalance (Olweus, 1993). Bullying is identified as one of the most predominant problems faced by children in the United States education system (Cantu & Heumann, 2000), as well as one of the most significant health risks to children (Cantu & Heumann, 2000; Espelage & Swearer, 2003; Rigby, Smith, & Pepler, 2004). Exactly how prevalent this issue is among students with disabilities is unclear because research focusing on this cohort is limited. However, most experts agree that children with disabilities are harassed by peers at higher rates than their peers without disabilities (Modell, 2005; Modell, Mak, & Jackson, 2004; Rose, Espelage, Stein, & Elliot, 2009; Sullivan & Knutson, 2000). Bullying can have a profound impact on students' performance, emotional health,
Calcium Supplementation Improves Na+/K+ Ratio, Antioxidant Defense and Glyoxalase Systems in Salt-Stressed Rice Seedlings

The present study investigates the regulatory role of exogenous calcium (Ca) in developing salt stress tolerance in rice seedlings. Hydroponically grown 13-day-old rice (Oryza sativa L. cv. BRRI dhan47) seedlings were exposed to 200 mM NaCl alone and combined with 2 mM CaCl2 and 2 mM ethylene glycol tetraacetic acid (EGTA, a Ca scavenger) for 3 days. The salt stress caused growth inhibition, chlorosis and water shortage in the rice seedlings. The salt-induced stress disrupted ion homeostasis through Na+ influx and K+ efflux, and decreased other mineral nutrient uptake. Salt stress caused oxidative stress in seedlings through lipid peroxidation, loss of plasma membrane integrity, higher reactive oxygen species (ROS) production and methylglyoxal (MG) formation. The salt-stressed seedlings supplemented with exogenous Ca recovered from water loss, chlorosis and growth inhibition. Calcium supplementation in the salt-stressed rice seedlings improved ion homeostasis by inhibition of Na+ influx and K+ leakage. Exogenous Ca also improved ROS and MG detoxification by improving the antioxidant defense and glyoxalase systems, respectively. On the other hand, applying EGTA along with salt and Ca again negatively affected the seedlings as EGTA negated Ca activity. It confirms that, the positive responses in salt-stressed rice seedlings to exogenous Ca were for Ca mediated improvement of ion homeostasis, antioxidant defense and glyoxalase system.
In situ expression of transforming growth factor-beta1-3, latent transforming growth factor-beta binding protein and tumor necrosis factor-alpha in liver tissue from patients with chronic hepatitis C.

BACKGROUND The mechanisms determining liver damage in chronic hepatitis C remain unclear. The aim was to evaluate the in situ expression of transforming growth factor beta (TGF-beta) and tumor necrosis factor-alpha (TNF-alpha), two key cytokines implicated as important pathogenic mediators in the development of liver fibrosis. METHODS In situ expression of TNF-alpha and of TGF-beta isoforms 1-3, and its transport protein latent TGF-beta binding protein (LTBP), was determined by immunohistochemistry in 9 untreated patients with chronic hepatitis C infection and in 6 controls without liver disease. In addition, TGF-beta1 expression was analyzed in 10 HCV patients before and after treatment with interferon-alpha alone, or in combination with ribavirin. RESULTS Liver biopsies from HCV patients showed positive staining for TGF-beta1-3 isoforms and LTBP, and to a lesser degree for TNF-alpha, in areas with inflammation and fibrosis. Normal control liver showed no positive staining. TGF-beta1 expression before treatment, quantified by morphometric analysis, did not differ between non-responders and sustained responders. In patients responding to therapy, TGF-beta1 expression decreased in parallel with histological improvement, while no difference in TGF-beta1 expression was seen before and after treatment in non-responders. CONCLUSION These results suggest that TNF-alpha and all three isoforms of TGF-beta are involved in the pathogenesis of HCV related liver disease, and that treatment leading to eradication of the virus affects the expression of TGF-beta1.
Integrin signaling in inflammatory and neuropathic pain in the rat

Many painful conditions are associated with alterations in the extracellular matrix (ECM) of affected tissues. While several integrins, the receptors for ECM proteins, are present on sensory neurons that mediate pain, the possible role of these cell adhesion molecules in inflammatory or neuropathic pain has not been explored. We found that the intradermal injection of peptide fragments of domains of laminin and fibronectin important for adhesive signaling selectively inhibited the hyperalgesia caused by prostaglandin E2 (PGE2) and epinephrine (EPI), respectively. The block of EPI hyperalgesia was mimicked by other peptides containing the RGD integrin‐binding sequence. Monoclonal antibodies (mAbs) against the α1 or α3 integrin subunits, which participate in laminin binding, selectively blocked PGE2 hyperalgesia, while a mAb against the α5 subunit, which participates in fibronectin binding, blocked only EPI‐induced hyperalgesia. A mAb against the β1 integrin subunit, common to receptors for both laminin and fibronectin, inhibited hyperalgesia caused by both agents, as did the knockdown of β1 integrin expression by intrathecal injection of antisense oligodeoxynucleotides. The laminin peptide, but not the fibronectin peptides, also reversibly abolished the longer lasting inflammatory hyperalgesia induced by carrageenan. Finally, the neuropathic hyperalgesia caused by systemic administration of the cancer chemotherapy agent taxol was reversibly inhibited by antisense knockdown of β1 integrin. These results strongly implicate specific integrins in the maintenance of inflammatory and neuropathic hyperalgesia.
WFPC2 Images of the Central Regions of Early-Type Galaxies. I. The Data

We present high-resolution R-band images of the central regions of 67 early-type galaxies obtained with the Wide Field and Planetary Camera 2 (WFPC2) aboard the Hubble Space Telescope (HST). This homogeneously selected sample roughly doubles the number of early-type galaxies that have now been imaged at HST resolution and complements similar data on the central regions of radio galaxies and the bulges of spiral galaxies. Our sample strikingly confirms the complex morphologies of the central regions of early-type galaxies which have become apparent from previous studies with HST. In particular, we detect dust, either in the form of nuclear disks or with a filamentary distribution, in 43% of all galaxies, in good agreement with previous estimates. In addition, we find evidence for embedded stellar disks in a remarkably large fraction of 51%. In 14 of those galaxies the disklike structures are misaligned with the main galaxy, suggesting that they correspond to stellar bars in S0 galaxies. We analyze the luminosity profiles of the galaxies in our sample and classify galaxies according to their central cusp slope. To a large extent we confirm the results from previous HST surveys in that early-type galaxies reveal a clear dichotomy: the bright ellipticals (MB ≲ -20.5) are generally boxy and have luminosity profiles that break from steep outer power laws to shallow inner cusps (referred to as "core" galaxies). The fainter ellipticals, on the other hand, typically have disky isophotes and luminosity profiles that lack a clear break and have a steep central cusp (referred to as "power-law" galaxies). The advantages and shortcomings of classification schemes utilizing the extrapolated central cusp slope γ are discussed, and it is shown that γ might be an inadequate representation for galaxies whose luminosity profile slope changes smoothly with radius rather than resembling a broken power law. Thus, we introduce a new, alternative parameter and show how this affects the classification. In fact, we find evidence for an "intermediate" class of galaxies that cannot unambiguously be classified as either core or power-law galaxies and that have central cusp slopes and absolute magnitudes intermediate between those of core and power-law galaxies. It is unclear at present, however, whether these galaxies make up a physically distinct class or whether distance and/or resolution effects cause them to lose their distinct core or power-law characteristics.
Is There a Relationship between the Stratum Corneum Thickness and That of the Viable Parts of Tumour Cells in Basal Cell Carcinoma?

Basal cell carcinoma (BCC) is an invasive epithelial skin tumour. The thickness of the outermost epidermal layer of the skin, the stratum corneum (SC), influences drug uptake and penetration into tumour and may thereby affect the response of BCC to topical treatment. The aim was to investigate a possible relationship between the thickness of the SC and that of the viable part of BCC. Histopathological evaluations of the corresponding SC and viable tumour thickness measurements of individual BCCs of different subtypes were explored. A total of 53 BCCs from 46 patients were studied. The median tumour thickness was 1.7 mm (0.8–3.0 mm), with a significant difference between subtypes (p < 0.001). The SC had a median thickness of 0.3 mm (0.2–0.4 mm), with no difference between tumour subtypes (p = 0.415). Additionally, no significant association between the thickness of the SC and that of the viable part of the tumour was demonstrated (p = 0.381). In conclusion our results indicate that SC thickness is relatively constant in BCC.
The impact of parents' demographic and psychological characteristics and parent involvement on young children's reading and math outcomes

Parents' involvement in children's education as a means to increase children's academic achievement has received national attention due to findings from studies and current educational legislation. The current study explores the impact of parents' demographic and psychological characteristics and their involvement in activities both school and at home on children's reading and math outcomes within the framework of Bronfenbrenner's bioecological theory. Using data from the Early Childhood Longitudinal Study, Kindergarten Class of 1998-99 (ECLS-K), a nationally representative sample of children and families, this study addresses the following research questions: what are the effects of parents' characteristics on child outcomes and on parent involvement, what is the effect of parent involvement on child outcomes, and to what extent does parent involvement mediate the association between parent characteristics and child outcomes. Results from the study revealed that parents' level of education and income were associated with both parent involvement and children's reading and math outcomes. In addition, parents' beliefs about their children's academic abilities also were strongly predictive of children's outcomes. Parent involvement in school was positively associated with children's reading and math outcomes, whereas parent involvement at home was negatively associated with children's outcomes. Lastly, parent involvement in school was found to partially mediate the association between parents' education and children's reading and math outcomes. This study highlights the impact parents have on their children's academic outcomes and findings suggest that programs aimed at helping parents build human capital is an important way to increase parent involvement at school and help children to succeed.
WRN at telomeres: implications for aging and cancer

Werner Syndrome (WS) is a premature aging syndrome characterized by early onset of age-related pathologies and cancer. Since WS is due to a single gene defect, it has attracted much interest from researchers seeking to understand pathways that contribute to cancer and aging at cellular and molecular levels. The protein mutated in WS, WRN, appears to play a major role in genome stability, particularly during DNA replication and telomere metabolism. Much of the pathophysiology associated with WS, including the rapid onset of cellular senescence, early cancer onset and premature aging, can be attributed to a defect in telomere maintenance. Recent genetic evidence from the mTerc–/– Wrn–/– mouse demonstrates that mice with critically shortened telomeres display aging phenotypes reminiscent of human WS, further reinforcing the notion that telomere dysfunction is required for the manifestation of aging pathophysiologies in the setting of WRN deficiency.