Miracle-dz/UniFAR-FTUs huggingface dataset:
CD133 positive progenitor endothelial cell lines from human cord blood

Endothelial progenitor cells (EPCs) modulate postnatal vascularization and contribute to vessel regeneration in adults. Stem cells and progenitor cells were found in umbilical cord blood, bone marrow, and mobilized peripheral blood cells, from where they were isolated and cultured. However, the yield of progenitor cells is usually not sufficient for clinical application and the quality of progenitor cells varies. The aim of the study was the immortalization of early progenitor cells with high proliferative potential, capable to differentiate to EPCs and, further, toward endothelial cells. Two cell lines, namely HEPC‐CB.1 and HEPC‐CB.2 (human endothelial progenitor cells—cord blood) were isolated. As assessed by specific antibody labeling and flow cytometric analysis, they express a panel of stem cell markers: CD133, CD13, CD271, CD90 and also endothelial cell markers: CD202b, CD309 (VEGFR2), CD146, CD105, and CD143 but they do not present markers of finally differentiated endothelial cells: CD31, vWf, nor CD45 which is a specific hematopoietic cell marker. Using the multiplex Cytometric Bead Assay, the simultaneous production of proangiogenic cytokines IL8, angiogenin, and VEGF was demonstrated in normoxia and was shown to be increased by hypoxia. Both cell lines, similarly as mature endothelial cells, underwent in vitro pre‐angiogenic process, formed pseudovessel structures and present an accelerated angiogenesis in hypoxic conditions. To date, these are the first CD133 positive established cell lines from human cord blood cells. © 2011 International Society for Advancement of Cytometry
Sinistrality revisited: a perceptual-motor approach.

This study reports the relation between perceptual-motor functioning and hand-eye dominance on 237 female and 216 male Negro 48-month-old Ss. Performances of Ss falling into five hand-eye-dominance categories were compared on two perceptual-motor tasks and Stanford-Binet IQ. Results showed that left-hand-left-eye-dominant Ss gave significantly poorer performances on perceptual-motor and intellectual measures than all other hand-eye-dominant Ss except left-hand-right-eye-dominant Ss. There were no significant differences among comparisons of other hand-eye-dominance groups on these measures. Since the present investigation represents a relatively new approach to sinistrality, there is no direct empirical evidence for comparison; however, support was obtained for a previous study finding left-hand-left-eye-dominant Ss deficient in intellectual functioning. Although expressions of hand-eye dominance appear to be under neurological control, the underlying mechanism seems obscure. A neurological frame of reference is proposed, and further research on etiological factors is suggested.
MIMO Communications over Multi-Mode Optical Fibers: Capacity Analysis and Input-Output Coupling Schemes

We consider multi-input multi-output (MIMO) communications over multi-mode fibers (MMFs). Current MMF standards, such as OM3 and OM4, use fibers with core radii of 50 \mu m, allowing hundreds of modes to propagate. Unfortunately, due to physical and computational complexity limitations, we cannot couple and detect hundreds of data streams into and out of the fiber. In order to circumvent this issue, we present input-output coupling schemes that allow the user to couple and extract a reasonable number of signals from a fiber with many modes. This approach is particularly attractive as it is scalable; i.e., the fibers do not have to be replaced every time the number of transmitters or receivers is increased, a phenomenon that is likely to happen in the near future. We present a statistical channel model that incorporates intermodal dispersion, chromatic dispersion, mode dependent losses, mode coupling, and input-output coupling. We show that the statistics of the fiber's frequency response are independent of frequency. This simplifies the computation of the average Shannon capacity of the fiber. We also provide an input-output coupling strategy that leads to an increase in the overall capacity. This strategy can be used whenever channel state information (CSI) is available at the transmitter. We show that the capacity of an Nt by Nt MIMO system over a fiber with M>>Nt modes can approach the capacity of an Nt-mode fiber with no mode-dependent losses. We finally present a statistical input-output coupling model in order to quantify the loss in capacity when CSI is not available at the transmitter. It turns out that the loss, relative to Nt-mode fibers, is minimal (less than 0.5 dB) for a wide range of signal-to-noise ratios (SNRs) and a reasonable range of MDLs.
Tenofovir during pregnancy in rats: a novel pathway for programmed hypertension in the offspring.

OBJECTIVES To evaluate the occurrence of systemic and renal abnormalities in the offspring of Wistar rats exposed to tenofovir disoproxil fumarate (DF) during pregnancy. METHODS Female Wistar rats received a standard diet, with or without addition of tenofovir DF (100 mg/kg diet), 1 week before mating and during pregnancy. Offspring from the tenofovir DF group were placed with an untreated foster mother during breastfeeding and compared with offspring from rats maintained on a standard diet during mating and pregnancy (control). Control and tenofovir DF were followed up at 3 and 6 months of age. Monthly body weight and systolic blood pressure (SBP), glomerular counts, renal function, biochemical parameters, angiotensin II, renal renin angiotensin aldosterone system (RAAS) and renal sodium transporters were analysed. RESULTS Tenofovir DF offspring showed lower birth weight compared with the control group. After the third month, growth among the tenofovir DF group experienced a rapid catch-up. SBP increased progressively after the second month of age in the tenofovir DF group. Nephron number did not differ between the groups; however, the tenofovir DF group showed glomerular structural changes. Plasma aldosterone was higher in the tenofovir DF group, associated with a significant increase in renal expression of RAAS. The tenofovir DF rats showed up-regulation of renal sodium transporters and consequently lower urinary sodium excretion. CONCLUSIONS This is the first demonstration using an experimental model that maternal exposure to tenofovir DF during gestation results in overactivation of RAAS, up-regulation of renal sodium transporters and hypertension in the offspring.
Proteomic Analysis of the Rat Canalicular Membrane Reveals Expression of a Complex System of P4-ATPases in Liver

Transport processes in the canalicular membrane are key elements in bile formation and are the driving force of the enterohepatic circulation of bile salts. The canalicular membrane is constantly exposed to the detergent action of bile salts. One potential element protecting the canalicular membrane from the high canalicular bile salt concentrations may be bile salt resistant microdomains, however additional factors are likely to play a role. To obtain more insights into the molecular composition of the canalicular membrane, the proteome of highly purified rat canalicular membrane vesicles was determined. Isolated rat canalicular membrane vesicles were stripped from adhering proteins, deglycosylated and protease digested before subjecting the samples to shot gun proteomic analysis. The expression of individual candidates was studied by PCR, Western blotting and immunohistochemistry. A total of 2449 proteins were identified, of which 1282 were predicted to be membrane proteins. About 50% of the proteins identified here were absent from previously published liver proteomes. In addition to ATP8B1, four more P4-ATPases were identified. ATP8A1 and ATP9A showed expression specific to the canalicular membrane, ATP11C at the bLPM and ATP11A in an intracellular vesicular compartment partially colocalizing with RAB7A and EEA1 as markers of the endosomal compartment. This study helped to identify additional P4-ATPases from rat liver particularly in the canalicular membrane, previously not known to be expressed in liver. These P4-ATPases might be contributing for maintaining transmembrane lipid homeostasis in hepatocytes.
Current and future perspectives on lumbar degenerative disc disease: a UK survey exploring specialist multidisciplinary clinical opinion

Objectives Despite lumbar degenerative disc disease (LDDD) being significantly associated with non-specific low back pain and effective treatment remaining elusive, specialist multidisciplinary clinical stakeholder opinion remains unexplored. The present study examines the views of such experts. Design A reliable and valid electronic survey was designed to establish trends using theoretical constructs relating to current assessment and management practices. Clinicians from the Society of Back Pain Research (SBPR) UK were invited to take part. Quantitative data were collated and coded using Bristol Online Surveys (BOS) software, and content analysis was used to systematically code and categorise qualitative data. Setting Specialist multidisciplinary spinal interest group in the UK. Participants 38/141 clinically active, multidisciplinary SBPR members with specialist spinal interest participated. Among them, 84% had >9 years postgraduate clinical experience. Interventions None. Outcome measures Frequency distributions were used to establish general trends in quantitative data. Qualitative responses were coded and categorised in relation to each theme and percentage responses were calculated. Results LDDD symptom recurrence, in the absence of psychosocial influence, was associated with physical signs of joint stiffness (26%), weakness (17%) and joint hypermobility (6%), while physical factors (21%) and the ability to adapt (11%) were postulated as reasons why some experience pain and others do not. No one management strategy was supported exclusively or with consensus. Regarding effective modalities, there was no significant difference between allied health professional and medic responses (p=0.1–0.8). The future of LDDD care was expressed in terms of improvements in patient communication (35%), patient education (38%) and treatment stratification (24%). Conclusions Results suggest that multidisciplinary expert spinal clinicians appear to follow UK-based assessment guidelines with regard to recurrent LDDD; there are, however, inconsistencies in the management approaches supported. This reflects the current literature and the lack of specific, formalised guidance. LDDD treatment stratification and further research are explicitly supported.
Observation of isotropic critical spin fluctuations in Gd.

Perturbed . gamma gamma. angular-correlation experiments on oriented single crystals of Gd, containing 1 ppb of /sup 111/In, demonstrate that critical spin fluctuations are isotropic to within about 1 K above the Curie temperature. Closer to T/sub C/ we find evidence that the critical spin fluctuations fall increasingly along the c axis. Analysis of nuclear relaxation times in the isotropic region leads to spin autocorrelation times that diverge with an exponent w = 0.53(8). This value is not consistent with either spin-nonconserved Heisenberg behavior or Ising behavior, but agrees with Moessbauer data for Gd /sup 161/Dy.
Origin and Length Distribution of Unidirectional Prokaryotic Overlapping Genes

Prokaryotic unidirectional overlapping genes can be originated by disrupting and replacing of the start or stop codon of one protein-coding gene with another start or stop codon within the adjacent gene. However, the probability of disruption and replacement of a start or stop codon may differ significantly depending on the number and redundancy of the start and stop codons sets. Here, we performed a simulation study of the formation of unidirectional overlapping genes using a simple model of nucleotide change and contrasted it with empirical data. Our results suggest that overlaps originated by an elongation of the 3′-end of the upstream gene are significantly more frequent than those originated by an elongation of the 5′-end of the downstream gene. According to this, we propose a model for the creation of unidirectional overlaps that is based on the disruption probabilities of start codon and stop codon sets and on the different probabilities of phase 1 and phase 2 overlaps. Additionally, our results suggest that phase 2 overlaps are formed at higher rates than phase 1 overlaps, given the same evolutionary time. Finally, we propose that there is no need to invoke selection to explain the prevalence of long phase 1 unidirectional overlaps. Rather, the overrepresentation of long phase 1 relative to long phase 2 overlaps might occur because it is highly probable that phase 2 overlaps are retained as short overlaps by chance. Such a pattern is stronger if selection against very long overlaps is included in the model. Our model as a whole is able to explain to a large extent the empirical length distribution of unidirectional overlaps in prokaryotic genomes.
“Not Out to Start a Revolution”

In this article, I use in-depth interviews with black university men to investigate race, gender, and emotions. Participation in dominant institutions requires African American men to exhibit extraordinary emotional restraint. Because anger is culturally associated with men, however, black men’s suppression of anger violates masculine expectations. Thus, racial subordination not only creates difficult emotional expectations but may also create emotional dilemmas in which expected emotional displays undermine other identity expectations. In this article, I examine both how a group of black university men achieve emotional restraint and how they use their emotions to craft and manage their identities as black middle-class men. I argue that black men distance themselves from the controlling image of the angry black man by developing a shared identity I call moderate blackness. Moderate blackness entails emotional restraint, a moderate approach to campus racial politics, and the ability to get along with white people. These strategies work together to produce positive, restrained emotions and to manage anger and agitation, but they require black university men to “not see” racism. Black men use defensive othering to push the stereotype of the angry black onto black women. In doing so, they shore up their masculinity but leave women responsible for combating racial inequality.
Mobility, fixity, agency: Australia’s working holiday programme

Recent writings on transnational corporeal mobility have been dominated by at least two concerns: mobility as ever-expanding or even complete; and mobility as determined by economic restructuring. In this paper, I confront such writings with empirical material from primarily qualitative research into Australia’s working holiday programme, which was established in 1975 to allow British citizens between the ages of 18 and 26 to work and holiday in Australia for a period of up to 12 months. And I confront such writings with M. P. Smith’s (2001) agency-oriented approach to transnational urbanism, which I extend with two arguments. Firstly, since agency is not a simple possession of intent or motivated human beings, the achievement of mobility rests on both human actors and non-human actants. Secondly, since mobility is not simply increasing, the achievement of mobility rests alongside what we might call relative contingent fixity.
Acral myxoinflammatory fibroblastic sarcoma: case series and immunohistochemical analysis

Background: Acral myxoinflammatory fibroblastic sarcoma (AMFS) is a rare, low‐grade neoplasm most often occurring on the extremities of adults. It consists of mixed inflammatory infiltrates with nodules of epithelioid, spindled and bizarre‐appearing cells within a fibrosclerotic‐to‐myxoid stroma. AMFS frequently recurs, but only rarely metastasizes.
A Randomized Controlled Trial of Psychoanalytic Psychotherapy or Cognitive-Behavioral Therapy for Bulimia Nervosa

Objective:The authors compared psychoanalytic psychotherapy and cognitive-behavioral therapy (CBT) in the treatment of bulimia nervosa.Method:A randomized controlled trial was conducted in which 70 patients with bulimia nervosa received either 2 years of weekly psychoanalytic psychotherapy or 20 sessions of CBT over 5 months. The main outcome measure was the Eating Disorder Examination interview, which was administered blind to treatment condition at baseline, after 5 months, and after 2 years. The primary outcome analyses were conducted using logistic regression analysis.Results:Both treatments resulted in improvement, but a marked difference was observed between CBT and psychoanalytic psychotherapy. After 5 months, 42% of patients in CBT (N=36) and 6% of patients in psychoanalytic psychotherapy (N=34) had stopped binge eating and purging (odds ratio=13.40, 95% confidence interval [CI]=2.45–73.42; p<0.01). At 2 years, 44% in the CBT group and 15% in the psychoanalytic psychotherapy group had stopped bing.
Massilia timonae gen. nov., sp. nov., Isolated from Blood of an Immunocompromised Patient with Cerebellar Lesions

ABSTRACT A fastidious, slowly growing, strictly aerobic, gram-negative bacterium was isolated from a culture of blood from a 25-year-old man with common variable immunodeficiency. The man had been admitted to hospital with febrile progressive cerebellar ataxia. The use of standard phenotypic schemes did not lead to identification, but sequence analysis demonstrated that the 16S rRNA gene of the isolate was most similar to those of the environmental bacteriaDuganella zoogloeoides (formerly Zoogloea ramigera 115) and Telluria mixta. Further characterization of the bacterium by biochemical analysis, electron microscopy, G+C content estimation, and fatty acid analysis demonstrated significant differences between the bacterium and D. zoogloeoides and Telluria species; thus, we propose it as a new taxon with the name Massilia timonae gen. nov., sp. nov.
Developmental Dynamics of X-Chromosome Dosage Compensation by the DCC and H4K20me1 in C. elegans

In Caenorhabditis elegans, the dosage compensation complex (DCC) specifically binds to and represses transcription from both X chromosomes in hermaphrodites. The DCC is composed of an X-specific condensin complex that interacts with several proteins. During embryogenesis, DCC starts localizing to the X chromosomes around the 40-cell stage, and is followed by X-enrichment of H4K20me1 between 100-cell to comma stage. Here, we analyzed dosage compensation of the X chromosome between sexes, and the roles of dpy-27 (condensin subunit), dpy-21 (non-condensin DCC member), set-1 (H4K20 monomethylase) and set-4 (H4K20 di-/tri-methylase) in X chromosome repression using mRNA-seq and ChIP-seq analyses across several developmental time points. We found that the DCC starts repressing the X chromosomes by the 40-cell stage, but X-linked transcript levels remain significantly higher in hermaphrodites compared to males through the comma stage of embryogenesis. Dpy-27 and dpy-21 are required for X chromosome repression throughout development, but particularly in early embryos dpy-27 and dpy-21 mutations produced distinct expression changes, suggesting a DCC independent role for dpy-21. We previously hypothesized that the DCC increases H4K20me1 by reducing set-4 activity on the X chromosomes. Accordingly, in the set-4 mutant, H4K20me1 increased more from the autosomes compared to the X, equalizing H4K20me1 level between X and autosomes. H4K20me1 increase on the autosomes led to a slight repression, resulting in a relative effect of X derepression. H4K20me1 depletion in the set-1 mutant showed greater X derepression compared to equalization of H4K20me1 levels between X and autosomes in the set-4 mutant, indicating that H4K20me1 level is important, but X to autosomal balance of H4K20me1 contributes only slightly to X-repression. Thus H4K20me1 by itself is not a downstream effector of the DCC. In summary, X chromosome dosage compensation starts in early embryos as the DCC localizes to the X, and is strengthened in later embryogenesis by H4K20me1.
Differentiated B lymphocytes. Potential to express particular antibody variable and constant regions depends on site of lymphoid tissue and antigen load

B cells have the potential to respond to an antigen by producing antibodies with a variety of variable and constant regions. We have quantitatively analyzed B-cell potential at the single cell level to determine the effect of lymphoid tissue site and antigen load on the expression of variable and constant regions. Concerning variable region expression, although the total frequency of B-cell precursors for phosphorylcholine is similar between nonimmune spleen and gut- associated Peyer's patch tissues, the proportion of cells producing non- TEPC 15 idiotypes is greater from Peyer's patch than from spleen. Oral immunization with phosphorylcholine-containing Ascaris suum increased the frequency of non-TEPC 15 B cells. Thus variation in the proportion of cells bearing different variable regions may be related to the distinct antigenic environment of cells in Peyer's patches compared to that of cells in spleen. Regarding constant region expression, although B cells from both spleen and Peyer's patches generate clones producing IgM, IgGl, and IgA singly and in all combinations, cells from Peyer's patches generate more clones secreting only IgA than cells from spleen. B cells specific for phosphorylcholine and inulin, which are found on intestinal bacteria, produce more IgA-only clones than B cells specific for the dinitrophenyl determinant. This striking correlation between IgA expression and variable region specificity for antigen implies that environmental antigens have expanded certain B cells in Peyer's patches which then have the ability to generate progeny that express only IgA. Evidence supporting the secondary nature of precursors for IgA-only clones is obtained by their ability to produce this isotype after stimulation with histoincompatible T cells. The role of gut antigens may be to clonally expand IgA precursors and perhaps to stimulate the proliferation of less differentiated cells within the unique microenvironment of the Peyer's patches, allowing them to differentiate to IgA precursors.
A COMPARATIVE STUDY OF KNOTS OF STAR FORMATION IN INTERACTING VERSUS SPIRAL GALAXIES

Interacting galaxies are known to have higher global rates of star formation on average than normal galaxies, relative to their stellar masses. Using UV and IR photometry combined with new and published Hα images, we have compared the star formation rates (SFRs) of ∼700 star forming complexes in 46 nearby interacting galaxy pairs with those of regions in 39 normal spiral galaxies. The interacting galaxies have proportionally more regions with high SFRs than the spirals. The most extreme regions in the interacting systems lie at the intersections of spiral/tidal structures, where gas is expected to pile up and trigger star formation. Published Hubble Space Telescope images show unusually large and luminous star clusters in the highest luminosity regions. The SFRs of the clumps correlate with measures of the dust attenuation, consistent with the idea that regions with more interstellar gas have more star formation. For the clumps with the highest SFRs, the apparent dust attenuation is consistent with the Calzetti starburst dust attenuation law. This suggests that the high luminosity regions are dominated by a central group of young stars surrounded by a shell of clumpy interstellar gas. In contrast, the lower luminosity clumps are bright in the UV relative to Hα, suggesting either a high differential attenuation between the ionized gas and the stars, or a post-starburst population bright in the UV but faded in Hα. The fraction of the global light of the galaxies in the clumps is higher on average for the interacting galaxies than for the spirals. Thus either star formation in interacting galaxies is “clumpier” on average, or the star forming regions in interacting galaxies are more luminous, dustier, or younger on average.