Miracle-dz/UniFAR-FTUs huggingface dataset:
Photosynthetic pigment-protein complexes as highly connected networks: implications for robust energy transport

Photosynthetic pigment-protein complexes (PPCs) are a vital component of the light-harvesting machinery of all plants and photosynthesizing bacteria, enabling efficient transport of the energy of absorbed light towards the reaction centre, where chemical energy storage is initiated. PPCs comprise a set of chromophore molecules, typically bacteriochlorophyll species, held in a well-defined arrangement by a protein scaffold; this relatively rigid distribution leads to a viewpoint in which the chromophore subsystem is treated as a network, where chromophores represent vertices and inter-chromophore electronic couplings represent edges. This graph-based view can then be used as a framework within which to interrogate the role of structural and electronic organization in PPCs. Here, we use this network-based viewpoint to compare excitation energy transfer (EET) dynamics in the light-harvesting complex II (LHC-II) system commonly found in higher plants and the Fenna-Matthews-Olson (FMO) complex found in green sulfur bacteria. The results of our simple network-based investigations clearly demonstrate the role of network connectivity and multiple EET pathways on the efficient and robust EET dynamics in these PPCs, and highlight a role for such considerations in the development of new artificial light-harvesting systems.
Very low frequency oscillations in the power spectra of heart rate variability during dry supine immersion and exposure to non-hypoxic hypobaria

The origin of very low frequency (VLF) oscillations in the power spectra of heart rate variability (HRV) is controversial with possible mechanisms involving thermoregulation and/or renin-angiotensin-aldosterone system. Recently, a major contribution from vagal influences has been suggested. The present study investigated the behaviour of VLF (0.004–0.040 Hz) components of HRV power spectra in a group of healthy male volunteers during their exposure to (1) dry, supine, immersion in thermo-neutral water for 6 h (n = 7) and (2) non-hypoxic hypobaria (breathing 40–60% oxygen at 15 000′ simulated in a decompression chamber) for 5 h (n = 15). The two manoeuvres are established to increase vagal outflow. During both the manoeuvres, all the frequency domain indices of HRV exhibited a significant increase. Increase in HRV was much more than that in the R-R interval. At 6 h of immersion, the R-R interval increased by ∼15% but the total power increased ∼fourfold. Similarly, at 5 h of exposure to hypobaria, total power increased ∼twofold with a very modest increase in an R-R of ∼9%. Increase in spectral power was appreciable even after normalization with mean R-R2. Increase in VLF during immersion was more than reported during enalaprilat blockade of angiotensin convertase enzyme. Plasma renin activity did not vary during hypobaria. There was a significant increase in pNN50, an established marker of cardiac vagal activity. Centre frequencies of the spectra and slope (β) of the relation between log(PSD) and log(frequency) did not change. Results were supportive of the notion that the parasympathetic system is pre-potent to influence slower (than respiratory) frequency components in HRV spectrum. Additionally, such an effect was without a change in the time constant of effector responses or pacemaker frequencies of VLF and LF periodicities and HRV was not a simple linear surrogate for cardiac vagal effects. An invariance of spectral exponent (β) ruled out contamination of VLF and LF spectra from fractal power as an alternate explanation.
Interference of contaminated sediment extracts and environmental pollutants with retinoid signaling

Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis. Some effects, such as malformations in frogs or changes in metabolism of birds, could be related to disruption of the retinoid signaling pathway by exposure to organic contaminants. A new reporter gene assay has been established for evaluation of the modulation of retinoid signaling by individual chemicals or environmental samples. The bioassay is based on the pluripotent embryonic carcinoma cell line P19 stably transfected with the firefly luciferase gene under the control of a retinoic acid‐responsive element (clone P19/A15). The cell line was used to characterize the effects of individual chemicals and sediments extracts on retinoid signaling pathways. The extracts of sediments from the River Kymi, Finland, which contained polychlorinated dioxins and furans and polycyclic aromatic hydrocarbons (PAHs), significantly increased the potency of all‐trans retinoic acid (ATRA), while no effect was observed with the extract of the sediment from reference locality. Considerable part of the effect was caused by the labile fraction of the sediment extracts. Also, several individual PAHs potentiated the effect of ATRA; on the other hand, 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin and several phthalates showed slightly inhibiting effect. These results suggest that PAHs could be able to modulate the retinoid signaling pathway and that they could be responsible for a part of the proretinoid activity observed in the sediment extracts. However, the effects of PAHs on the retinoic acid signaling pathways do not seem to be mediated directly by crosstalk with aryl hydrocarbon receptor.
Maternal HIV‐1 Antibody and Vertical Transmission in Subtype C Virus Infection

Summary: The role of maternal humoral immune response and viral load was analyzed in relation to the incidence of mother‐to‐child transmission (MTCT) of infants born to HIV‐1 subtype C infected mothers. High levels of viral RNA in the serum correlated with MTCT as did high titers of subtype C consensus V3 peptide binding antibodies (BA) and neutralizing antibody (NA) to subtype B HIV‐1MN. Logistic regression analysis showed that maternal viral load and V3 peptide subtype C BA were independent predictors for MTCT, odds ratio (OR) = 2.22 and OR = 2.52, respectively. No correlation between NA to homologous HIV‐1 subtype C virus and MTCT was found. BA to V3 peptides may provide a rapid inexpensive method that can be used to determine the risk of HIV‐1 MTCT.
Unintended consequences of lowering disclosure thresholds : proposed changes to SFAS no. 5

In recent years, regulators have considered several initiatives to lower the threshold for disclosing risks to investors. We examine two ways in which disclosing more risks can actually lower investors’ perceptions of risk. Utilizing an experiment, we find evidence of two unintended consequences on different types of investors. First, we demonstrate that the addition of low-probability risks to a disclosure can dilute (rather than add to) more probable losses, leading certain investors to lower their perceptions of overall risk. Second, since lowering the threshold changes the overall composition of the disclosure by adding low-probability losses, firms could adopt a tactic of minimization that characterizes the entire disclosure as unimportant, presenting the lowest risks most saliently, using compliance with the low threshold as a plausible reason for giving a lengthy disclosure of generally unimportant risks. Our findings suggest that such a tactic can be persuasive.
LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing

In recent years there have been major advances with respect to the identification of the protein components and mechanisms of microRNA (miRNA) mediated silencing. However, the complete and precise repertoire of components and mechanism(s) of action remain to be fully elucidated. Herein we reveal the identification of a family of three LIM domain-containing proteins, LIMD1, Ajuba and WTIP (Ajuba LIM proteins) as novel mammalian processing body (P-body) components, which highlight a novel mechanism of miRNA-mediated gene silencing. Furthermore, we reveal that LIMD1, Ajuba, and WTIP bind to Ago1/2, RCK, Dcp2, and eIF4E in vivo, that they are required for miRNA-mediated, but not siRNA-mediated gene silencing and that all three proteins bind to the mRNA 5′ m7GTP cap–protein complex. Mechanistically, we propose the Ajuba LIM proteins interact with the m7GTP cap structure via a specific interaction with eIF4E that prevents 4EBP1 and eIF4G interaction. In addition, these LIM-domain proteins facilitate miRNA-mediated gene silencing by acting as an essential molecular link between the translationally inhibited eIF4E-m7GTP-5′cap and Ago1/2 within the miRISC complex attached to the 3′-UTR of mRNA, creating an inhibitory closed-loop complex.
The impact of Acceptance and Commitment Therapy versus education on stigma toward people with psychological disorders

Acceptance and Commitment Therapy (ACT) has previously been shown to alter stigmatizing attitudes and to be relatively useful for psychologically inflexible participants. The present study is the first to bring those two findings together by comparing ACT to an education intervention for reducing stigma toward people with psychological disorders, and examining whether results differ for psychologically inflexible versus flexible individuals. A sample of college students (N = 95) was randomly assigned to a 2 1⁄2 hour ACT or educational workshop. Measures were taken before and after the workshop and at a one-month follow-up. ACT reduced mental health stigma significantly regardless of participants’ pre-treatment levels of psychological flexibility, but education reduced stigma only among participants who were relatively flexible and non-avoidant to begin with. Acceptance could be an important avenue of exploration for stigma researchers.
An Analysis of Synaptic Normalization in a General Class of Hebbian Models

In standard Hebbian models of developmental synaptic plasticity, synaptic normalization must be introduced in order to constrain synaptic growth and ensure the presence of activity-dependent, competitive dynamics. In such models, multiplicative normalization cannot segregate afferents whose patterns of electrical activity are positively correlated, while subtractive normalization can. It is now widely believed that multiplicative normalization cannot segregate positively correlated afferents in any Hebbian model. However, we recently provided a counterexample to this belief by demonstrating that our own neurotrophic model of synaptic plasticity, which can segregate positively correlated afferents, can be reformulated as a nonlinear Hebbian model with competition implemented through multiplicative normalization. We now perform an analysis of a general class of Hebbian models under general forms of synaptic normalization. In particular, we extract conditions on the forms of these rules that guarantee that such models possess a fixed-point structure permitting the segregation of all but perfectly correlated afferents. We find that the failure of multiplicative normalization to segregate positively correlated afferents in a standard Hebbian model is quite atypical.
Pilot clinical study of the effects of ginger root extract on eicosanoids in colonic mucosa of subjects at increased risk for colorectal cancer

Colorectal cancer (CRC) remains a significant cause of mortality. Inhibitors of cyclooxygenase (COX) and thus prostaglandin E2, are promising CRC preventives, but have significant toxicities. Ginger has been shown to inhibit COX, to decrease the incidence and multiplicity of adenomas, and decrease PGE2 concentrations in subjects at normal risk for CRC. This study was conducted to determine the effects of 2.0 g/d of ginger given orally on the levels of PGE2, leukotriene B4 (LTB4), 13‐hydroxy‐octadecadienoic acids, and 5‐, 12‐, & 15‐hydroxyeicosatetraenoic acid, in the colonic mucosa of subjects at increased risk for CRC. We randomized 20 subjects to 2.0 g/d ginger or placebo for 28 d. At baseline and Day 28, a flexible sigmoidoscopy was used to obtain colon biopsies. A liquid chromatography mass spectrometry method was used to determine eicosanoid levels in the biopsies, and levels were expressed per amount of protein or free arachidonic acid (AA). There was a significant decrease in AA between baseline and Day 28 (P = 0.05) and significant increase in LTB4 (P = 0.04) when normalized to protein, in subjects treated with ginger versus placebo. No other changes in eicosanoids were observed. There was no difference between the groups in total adverse events (AE; P = 0.06). Ginger lacks the ability to decrease eicosanoid levels in people at increased risk for CRC. Ginger did appear to be both tolerable and safe; and could have chemopreventive effects through other mechanisms. Further investigation should focus on other markers of CRC risk in those at increased CRC risk. © 2014 Wiley Periodicals, Inc.
Risk factors for high-order multiple implantation after ovarian stimulation with gonadotrophins: evidence from a large series of 1878 consecutive pregnancies in a single centre.

BACKGROUND High-order multiple pregnancies (triplets or more) have a large adverse impact on perinatal morbidity and mortality as well as important economic consequences. Most triplets and higher births are due to ovulation induction alone or in combination with intrauterine insemination (IUI) rather than to in-vitro fertilization (IVF). The present investigation was undertaken to determine whether there were specific variables that related to patient clinical characteristics (age of the woman, duration of infertility, type of infertility, body mass index, basal FSH and LH concentrations), treatment characteristics (initial dose of gonadotrophins, total dose of gonadotrophins administered, number of days of ovarian stimulation, insemination procedure, number of spermatozoa inseminated in patients undergoing IUI, type of luteal support), and ovarian response (oestradiol serum concentrations, number and size of follicles) that might be associated with the occurrence of high-order multiple implantation in order to develop a prediction model. METHODS This study employed univariate, multivariate and receiver-operating characteristic (ROC) analysis of a large series of 1878 consecutive pregnancies obtained in cycles stimulated with gonadotrophins. Of them, 1771 (94.3%) were low-order pregnancies (1477 singletons and 294 pairs of twins) and 107 (5.7%) were high-order pregnancies. RESULTS Predictive variables in the multivariate analysis were age of the woman, serum oestradiol concentrations and number of follicles >10 mm on the day of HCG injection. Stratification of the number of follicles into three categories (1 to 3, 4 to 5, and >5 follicles respectively), peak serum oestradiol and woman's age according to the ROC curves, showed that the risk of high-order multiple implantation correlated significantly with increasing total number of follicles and was significantly increased in women with a serum oestradiol >862 pg/ml and aged < or =32 years. CONCLUSIONS This three-variable model can help to identify patients at high-risk for high-order multiple pregnancy in ovulation induction cycles.
Mapping the U.S. West Coast surface circulation: A multiyear analysis of high‐frequency radar observations

signals with phase speeds of O(10) and O(100 to 300) km day −1 and time scales of 2 to 3 weeks. The signals with slow phase speed are only observed in southern California. It is hypothesized that they are scattered and reflected by shoreline curvature and bathymetry change and do not penetrate north of Point Conception. The seasonal transition of alongshore surfacecirculationforcedbyupwelling‐favorablewindsandtheirrelaxationiscapturedinfine detail.Submesoscaleeddies,identifiedusingflowgeometry,haveRossbynumbersof0.1to3, diameters in the range of 10 to 60 km, and persistence for 2 to 12 days. The HFR surface currents resolve coastal surface ocean variability continuously across scales from
Children With Attention Deficit Hyperactivity Disorder: Are There Gender Differences in School Functioning?

Few studies have comprehensively examined possible gender differences in the school functioning of children with attention deficit hyperactivity disorder (ADHD). This study investigated differences in academic, social, and emotional and behavioral functioning between 133 male and 42 female elementary school students who met research diagnostic criteria for ADHD. School functioning was assessed using teacher ratings, direct observations of classroom behavior, and a standardized, norm-referenced achievement test. Results indicated that participants, regardless of gender, experienced impairment across all functioning domains. The few gender differences obtained varied across areas of functioning and were dependent, in part, on the type of score and comparison group used. Specifically, although girls were less likely to have ADHD than were boys, when they did exhibit this disorder, their impairments were as severe, or possibly more severe, than for boys relative to non-ADHD peers of the same gender. Implications of these findings for school-based practice and research are discussed.
Intestinal parasites among presumably healthy individuals in Lebanon.

OBJECTIVE To determine the prevalence period of intestinal parasites among presumably healthy subjects in Lebanon. METHODS One stool specimen from 2634 presumably healthy Lebanese subjects, mean age 32.1 years with a range of 14-71 years, resident of different areas in Lebanon was examined for the presence of parasites. The analysis took place in the Clinical Microbiology Laboratory, Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon, over 25-months between 1995-1997. RESULTS The prevalence of intestinal parasites was 12.4%. The most common parasites identified were Entamoeba coli (38%), Giardia lamblia (3.1%) and Entamoeba histolytica (2.3%) [corrected]. CONCLUSION The data presented shows the need to improve hygienic conditions to contain the problem of intestinal infections with parasites in Lebanon.
Genomics and Proteomics

Abstract: Advances in genomics and proteomics increasingly contribute to the understanding of signal transduction pathways that control growth, differentiation, and death of cells. Since defects in these processes may result in the expression of inherited and or acquired disease, the identification of candidate disease genes and modifier genes by parallel use of genotyping together with an integrated study of gene expression and metabolite levels is instrumental for future health care. This approach, called systems biology, aims to recognize early onset of disease, institute preventive treatment, and identify new molecular targets for novel drugs in cancer, cardiovascular and metabolomic disease (e.g., diabetes), and neurodegenerative disorders. Gene interaction networks have recently been demonstrated, in which hub genes, that is, genes that show the highest level of interactions with other genes, play a special role. Hub genes, often chromatin regulators, may act as modifier genes (genes that modify the effect of other genes) in multiple mechanistically unrelated genetic diseases in humans. In addition, it has been shown that small metabolites such as hormones and cytokines, or proteins/enzymes such as C reactive protein (C‐RP) and matrix metaloproteinase (MMP), reflect disease status in case of oral cancer, asthma, or periodontal and cardiac disease. Many of these molecular targets, as well as pathogen‐specific DNA and RNA sequences, can be measured in oral fluids, providing a unique opportunity to develop novel noninvasive diagnostic tests. Efforts so far concentrate on the use of lab‐on‐a‐chip technology in combination with novel reporters and microsensor arrays to measure multianalytes in oral fluids. Handheld devices that perform sensitive detection of multiple analytes in oral fluid will be obtainable in the near future.
Emotional modulation of memory and cortical reactivation

During memory retrieval a subset of the brain regions active during encoding reactivate. The specificity of this reactivation and its interaction with emotion are still unclear. In four original studies we explored content-specificity, emotional modulation of memory reactivation in the cortex during conscious retrieval, as well as the impact of brain lesions on reactivation. The results show that reactivation reflects retrieved content (faces vs words) and is modulated by emotion. Lesions in the medial temporal lobe can impair such reactivation and disturb the emotional modulation. Our results also show that the reactivation follows the subjective memory experience rather than the activation related to the actual stimuli present at encoding. Finally we also measured reactivation for faces after emotional induction and found enhanced reactivation when the emotion of the induction and face expression were congruent. We conclude that cortical reactivations during conscious memory retrieval are content-specific and modulated by emotional contexts.
Nitric Oxide Interacts with Salicylate to Regulate Biphasic Ethylene Production during the Hypersensitive Response1[W]

C2H4 is associated with plant defense, but its role during the hypersensitive response (HR) remains largely uncharacterized. C2H4 production in tobacco (Nicotiana tabacum) following inoculation with HR-eliciting Pseudomonas syringae pathovars measured by laser photoacoustic detection was biphasic. A first transient rise (C2H4-I) occurred 1 to 4 h following inoculation with HR-eliciting, disease-forming, and nonpathogenic strains and also with flagellin (flg22). A second (avirulence-dependent) rise, at approximately 6 h (C2H4-II), was only seen with HR-eliciting strains. Tobacco leaves treated with the C2H4 biosynthesis inhibitor, aminoethoxyvinylglycine, suggested that C2H4 influenced the kinetics of a HR. Challenging salicylate hydroxylase-expressing tobacco lines and tissues exhibiting systemic acquired resistance suggested that C2H4 production was influenced by salicylic acid (SA). Disrupted expression of a C2H4 biosynthesis gene in salicylate hydroxylase tobacco plants implicated transcriptional control as a mechanism through which SA regulates C2H4 production. Treating leaves to increase oxidative stress or injecting with SA initiated monophasic C2H4 generation, but the nitric oxide (NO) donor sodium nitroprusside initiated biphasic rises. To test whether NO influenced biphasic C2H4 production during the HR, the NO synthase inhibitor NG-nitro-l-arginine methyl ester was coinoculated with the avirulent strain of P. syringae pv phaseolicola into tobacco leaves. The first transient C2H4 rise appeared to be unaffected by NG-nitro-l-arginine methyl ester, but the second rise was reduced. These data suggest that NO and SA are required to generate the biphasic pattern of C2H4 production during the HR and may influence the kinetics of HR formation.
Criminogene en beschermende factoren bij jongeren die een basisonderzoek ondergaan

De volgende onderzoeksvragen worden in deze rapportage beantwoord:In welke mate signaleren raadsonderzoekers aanwijzingen voor mogelijke psychosociale problemen bij jongeren die een basisraadsonderzoek ondergaan omdat ze door de politie verdacht worden van een strafbaar feit? Welke specifieke risico- en beschermende factoren komen voor bij jongeren die een basisraadsonderzoek ondergaan omdat ze door de politie verdacht worden van een strafbaar feit? Welke aanwijzingen zijn er voor mogelijke psychosociale problemen en risico- en beschermende factoren bij jongeren die een basisraadsonderzoek ondergaan vanwege een overtreding van de Leerplichtwet?