Miracle-dz/UniFAR-FTUs huggingface dataset:
Escherichia coli periplasmic chaperone FaeE is a homodimer and the chaperone‐K88 subunit complex is a heterotrimer

The interaction of FaeE, a periplasmic chaperone involved in K8B biosynthesis, and the major fimbrial subunit FaeG was Investigated. The genes encoding the two proteins were subcloned together in the expression vector pINIIIA1, Cells expressing the sub‐cloned genes accumulated in their periplasm a complex of FaeE and FaeG. This complex was purified by isoelectric focusing and anion‐exchange fast‐protein liquid chromatography. SOS‐PAGE, native gel etectrophoresis, immunoblotting and determination of the N‐terminal amino acid sequences and the molar ratio of the W‐terminal amino acid residues revealed that the complex is a heterotrimer consisting of two molecules of FaeE and one molecule of FaeG. The periplasmic chaperone FaeE was purified from the periplasm of cells expressing only the subcloned faeE gene. Gel filtration, protein cross‐linking analysis and a biophysical approach in which the rotation diffusion coefficient of the purified FaeE was determined led to the conclusion that the native FaeE chaperone is a homodimer.
Sintomatologia de depressão e suporte familiar em adolescentes: um estudo de correlação

In adolescence, there are many changes such as: physical, hormonal and psychological that could have a bearing on the development of an assortment of disorders and mental symptoms, for example depression symptomology, which the family can have an influence over by starting and maintaining the development of these symptoms. The objective of the research is to evaluate the correlation that exists between the depression symptomology and the perception of family support in high school students. One hundred and fifty-four (154) students from a public high school participated in the research. The materiais used were an identification questionnaire (with several risk factors in depression), the Children’s Depression Inventory (CDI) Scale, and the Parental Bonding Instrument (PBI). The questionnaires were given collectively in the classroom. The main results showed that the more an adolescent exhibited a depressive symptomology, the more inadequate the adolescent’s family support is, and thus a greater risk factor. In this sample of adolescents, the research can conclude that family support is negatively related to depressive symptomology.
RNPC1 modulates the RNA-binding activity of, and cooperates with, HuR to regulate p21 mRNA stability

P21, a cyclin-dependent kinase inhibitor, plays a pivotal role in the cell-cycle regulation in response to stress stimuli. P21 expression is highly regulated through transcriptional, post-transcriptional and post-translational mechanisms. Previously, we and others showed that p21 expression is regulated through p21 mRNA stability by RNPC1, a target of the p53 family and HuR, a member of the ELAV family RNA-binding proteins. HuR carries three highly conserved RNA recognition motifs (RRMs) whereas RNPC1 carries one. Here we found that the ability of RNPC1 to regulate p21 mRNA stability is dependent on HuR. We also found that RNPC1 and HuR physically interact, and the RRM domain in RNPC1 and RRM3 in HuR are necessary for their interaction. Interestingly, we found that RNPC1 and HuR, both of which can bind AU-rich elements (AREs) in p21 3′-UTR, preferentially bind the upstream and downstream AREs, respectively. Finally, we showed that the RNA-binding activity of HuR to p21 transcript was enhanced by RNPC1 in vitro and in vivo. Together, we hypothesize that RNPC1 modulates the RNA-binding activity of, and cooperates with, HuR to regulate p21 mRNA stability.
Effects of acute caffeine ingestion on resistance training performance and perceptual responses during repeated sets to failure.

AIM The aim of the present study was to evaluate the effect of oral caffeine ingestion during repeated sets of resistance. METHODS Fourteen moderately resistance-trained men (20.9 ± 0.36 years and 77.62 ± 2.07 kg of body weight) ingested a dose of caffeine (5 mg.kg-1) or placebo prior to 3 sets of bench press and 3 sets of leg press exercises, respectively. The study used a double-blind, counterbalanced, crossover design. Repetitions completed and total weight lifted were recorded in each set. Readiness to invest in both physical (RTIPE) and mental (RTIME) effort were assessed prior each set, and rating of perceived exertion (RPE) was recorded after each set. Rest and peak heart rates were determined via telemetry. RESULTS Caffeine ingestion result in increased number of repetitions to failure in bench press (F[1,13]=6.16, P=0.027) and leg press (F[1,13]=9.33, P=0.009) compared to placebo. The sum of repetitions performed in the 3 sets was 11.60% higher in bench press (26.86 ± 1.74; caffeine: 30.00 ± 1.87; P=0.027) and 19.10% in leg press (placebo: 40.0 ± 4.22; caffeine: 47.64 ± 4.69; P=0.009). Also, RTIME was increased in the caffeine condition both in bench press (F[1,13]=7.02, P=0.02) and in leg press (F[1,13]=5.41, P=0.03). There were no differences in RPE, RTIPE and HR (P>0.05) across conditions. CONCLUSION Acute caffeine ingestion can improve performance in repeated sets to failure and increase RTIME in resistance-trained men.
Aortic Remodelling Is Improved by 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside Involving the Smad3 Pathway in Spontaneously Hypertensive Rats

Hypertension is a common health problem that substantially increases the risk of cardiovascular disease. The condition increases blood pressure, which causes alterations in vascular structure and leads to the development of vascular pathologies. 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG), a resveratrol analogue extracted from a Chinese medicinal plant, has been proven to have numerous vascular protection functions. This study investigated whether THSG can improve vascular remodeling, which has thus far remained unclear. Orally administering THSG to spontaneously hypertensive rats (SHRs) aged 12 weeks for 14 weeks significantly inhibited intima-media thickness in the lower parts of the aortic arch, increased the vascular diastolic rate in response to acetylcholine, and reduced remodelling-related mRNA expression, such as that of ACTA2, CCL3, COL1A2, COL3A1, TIMP1 WISP2, IGFBP1, ECE1, KLF5, MYL1 BMP4, FN1, and PAI-1. Immunofluorescence staining also showed an inhibitory effect similar to that of THSG on PAI-1 protein expression in rat aortas. Results from immunoprecipitation and a Western blot assay showed that THSG inhibited the acetylation of Smad3. A chromatin immunoprecipitation assay showed that THSG prevented Smad3 binding to the PAI-1 proximal promoter in SHR aortas. In conclusion, our results demonstrated that the inhibitory effect of THSG on aortic remodelling involved the deacetylating role of Smad3 with increasing blood flow and with constant blood pressure.
Molecular rationale delineating the role of lycopene as a potent HMG-CoA reductase inhibitor: in vitro and in silico study

Abstract This study initially aimed to depict the molecular rationale evolving the role of lycopene in inhibiting the enzymatic activity of β-hydroxy-β-methylglutaryl-CoA (HMG-CoA) reductase via in vitro and in silico analysis. Our results illustrated that lycopene exhibited strong HMG-CoA reductase inhibitory activity (IC50 value of 36 ng/ml) quite better than pravastatin (IC50 = 42 ng/ml) and strong DPPH free radical scavenging activity (IC50 value = 4.57 ± 0.23 μg/ml) as compared to ascorbic acid (IC50 value = 9.82 ± 0.42 μg/ml). Moreover, the Ki value of lycopene (36 ng/ml) depicted via Dixon plot was well concurred with an IC50 value of 36 ± 1.8 ng/ml. Moreover, molecular informatics study showed that lycopene exhibited binding energy of −5.62 kcal/mol indicating high affinity for HMG-CoA reductase than HMG-CoA (ΔG: −5.34 kcal/mol). Thus, in silico data clearly demonstrate and support the in vitro results that lycopene competitively inhibit HMG-CoA reductase activity by binding at the hydrophobic portion of HMG-CoA reductase.
On the Temporal Characteristics of Performance Variability in Attention Deficit Hyperactivity Disorder (ADHD)

Increased intra-subject variability of reaction times (ISV-RT) is one of the most consistent findings in attention-deficit/hyperactivity disorder (ADHD). Although the nature of this phenomenon is still unclear, it has been hypothesised to reflect interference from the Default Mode Network (DMN). So far, ISV-RT has been operationally defined either as a frequency spectrum of the underlying RT time series, or as a measure of dispersion of the RT scores distribution. Here, we use a novel RT analysis framework to link these hitherto unconnected facets of ISV-RT by determining the sensitivity of different measures of RT dispersion to the frequency content of the underlying RT time series. N=27 patients with ADHD and N=26 healthy controls performed several visual N-back tasks. Different measures of RT dispersion were repeatedly modelled after individual frequency bands of the underlying RT time series had been either extracted or suppressed using frequency-domain filtering. We found that the intra-subject standard deviation of RT preserves the “1/f noise” characteristic typical of human RT data. Furthermore and most importantly, we found that the ex-Gaussian parameter τ is rather exclusively sensitive to frequencies below 0.025 Hz in the underlying RT time series and that the particularly slow RTs, which nourish τ, occur regularly as part of an quasi-periodic, ultra-slow RT fluctuation. Overall, our results are compatible with the idea that ISV-RT is modulated by an endogenous, slowly fluctuating process that may reflect DMN interference.
‘Tick the Box Please’: A Reflexive Approach to Doing Quantitative Social Research

This article considers the value of reflexivity in relation to a quantitative research project which we worked on as contract researchers. Reflexivity in the research process has been discussed by social scientists for over three decades. However, many quantitative researchers continue to avoid explicit forms of reflexivity, especially in relation to data collection. We discuss our specific experiences and also raise general questions about the application of reflexivity to quantitative methods. In addition, we outline the difficulties of being reflexive given the financial and time constraints of contract research. We consider this article a timely contribution to current discussions about the continuing growth of contract research and debates about the relevance of quantitative research methodologies for social scientists.
Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin‐producing Escherichia coli isolated from raw products in Argentina

A total of 73 Shiga toxin‐producing Escherichia coli (STEC) isolates, belonging to 25 serotypes and isolated from raw products in Argentina, were examined for the occurrence of genes responsible for bacterial adhesions to intestine, ehaA (EHEC autotransporter), lpfAO113 (long polar fimbriae), sab (STEC autotransporter [AT] contributing to biofilm formation), ecpA (E. coli common pilus), hcpA (haemorrhagic coli pilus), elfA (E. coli laminin‐binding fimbriae), sfpA (sorbitol‐fermenting EHEC O157 fimbriae plasmid‐encoded) and of the toxigenic gene cdt‐V (cytolethal distending toxin). Our study showed different adhesin profiles that are not linked to one specific serotype and that all analysed isolates possess, besides stx genes, some adherence genes. Several of the isolates contained also multiple toxin genes. The results of the present work alert the presence of genes coding for additional adhesins and cdt‐V toxin in LEE‐negative STEC strains that occur in foods, and this traits could increase their pathogenic potential.
Abatacept Therapy for Rheumatoid Arthritis in the Setting of Hepatitis C Infection

Chronic hepatitis C in the setting of rheumatoid arthritis may be an obstacle to optimal management because of potential hepatotoxicity and adverse effects from immune suppression in these patients with a chronic infection. There are no reports in the literature discussing the use of abatacept, a T-cell costimulatory blocker, in patients with chronic hepatitis C. This article is the first report to describe the use of abatacept in 2 patients with rheumatoid arthritis and concomitant hepatitis C with favorable outcome.
Bicultural Social Identity: Do Biculturals Assess Workplace Deviance Differently Across Different Ethnicities?

BICULTURAL SOCIAL IDENTITY: DO BICULTURALS ASSESS WORKPLACE DEVIANCE DIFFERENTLY ACROSS DIFFERENT ETHNICITIES? by Andy Y. Chiou Adviser: Professor Stephan Dilchert Research on biculturals has informed us much regard ing their cognition and identity integration, however little as to the bicultural perception of inand out-groups, and whether it can be primed. I examined this question in a workplace setting using the black sheep framework, specifically: Given the activation of one of a bicultural’s specific cultural identities, will he or she allocate punishment to d eviant employees of different cultural backgrounds differently based on whether he or she views them as an in-group or outgroup member? In Study 1, I show that Asian-American biculturals do assess deviant employees of different cultural backgrounds differently depending on the cultural prime, and that this effect is moderated by their level of bicultural identity integration. In Study 2, I show that Hispanic-American biculturals exhibit a contrastive response to cultural primes, which still supports the main hypotheses (S tudy 2). The reason for this difference between the two study samples, implications for bic ultural research, and organizational behavior research are discussed.
ReWiFlow: Restricted Wildcard OpenFlow Rules

The ability to manage individual flows is a major benefit of Software-Defined Networking. The overheads of this fine-grained control, e.g. initial flow setup delay, can overcome the benefits, for example when we have many time-sensitive short flows. Coarse-grained control of groups of flows, on the other hand, can be very complex: each packet may match multiple rules, which requires conflict resolution. In this paper, we present ReWiFlow, a restricted class of OpenFlow wildcard rules (the fundamental way to control groups of flows in OpenFlow), which allows managing groups of flows with flexibility and without loss of performance. We demonstrate how ReWiFlow can be used to implement applications such as dynamic proactive routing. We also present a generalization of ReWiFlow, called Multi-ReWiFlow, and show how it can be used to efficiently represent access control rules collected from Stanford's backbone network.
Auxin Signaling in Regulation of Plant Translation Reinitiation

The mRNA translation machinery directs protein production, and thus cell growth, according to prevailing cellular and environmental conditions. The target of rapamycin (TOR) signaling pathway—a major growth-related pathway—plays a pivotal role in optimizing protein synthesis in mammals, while its deregulation triggers uncontrolled cell proliferation and the development of severe diseases. In plants, several signaling pathways sensitive to environmental changes, hormones, and pathogens have been implicated in post-transcriptional control, and thus far phytohormones have attracted most attention as TOR upstream regulators in plants. Recent data have suggested that the coordinated actions of the phytohormone auxin, Rho-like small GTPases (ROPs) from plants, and TOR signaling contribute to translation regulation of mRNAs that harbor upstream open reading frames (uORFs) within their 5′-untranslated regions (5′-UTRs). This review will summarize recent advances in translational regulation of a specific set of uORF-containing mRNAs that encode regulatory proteins—transcription factors, protein kinases and other cellular controllers—and how their control can impact plant growth and development.
Lifestyle Intervention “Obeldicks Mini” for Obese Children Aged 4 to 7 Years

Interventions for obese preschool children are missing in Germany. However, an effective and long-lasting improvement of the health behaviour seems plausible especially in this age, since the health behaviour is impressed in this age span. Therefore, we developed the outpatient one-year lifestyle intervention "Obeldicks Mini" for obese children aged 4 to <8 years and their parents based on nutrition, education, physical activity, and behaviour therapy. This intervention addressed primarily the parents. The training program was evaluated in 84 patients. In the three months before intervention, the degree of overweight significantly increased in the participants (in mean+0.12 SDS-BMI; p=0.002). Based on an intention-to-treat approach, 69% of the participants reduced their overweight, while 24% dropped out of the intervention. The mean SDS-BMI reduction was 0.46 (p<0.001) and was associated with a significant improvement of cardiovascular risk factors such as hypertension, dyslipidemia and insulin resistance. Intima-media thickness as predictive factor for later atherosclerosis decreased significantly. Even 3 years after end of intervention, the achieved weight loss sustained.
Randomized prospective study comparing radical prostatectomy alone versus radical prostatectomy preceded by androgen blockade in clinical stage B2 (T2bNxM0) prostate cancer. The Lupron Depot Neoadjuvant Prostate Cancer Study Group.

PURPOSE Nonrandomized clinical trials have suggested that preoperative androgen deprivation can decrease the likelihood of positive surgical margins in patients with clinically localized prostate cancer. A multicenter prospective randomized trial compared radical prostatectomy alone to radical prostatectomy after 3 months of leuprolide acetate depot and flutamide in patients with stage cT2bNxM0 prostate cancer and a serum prostate specific antigen level less than 50 ng. /ml. MATERIALS AND METHODS We randomized 149 patients to undergo androgen deprivation and 138 to undergo lymphadenectomy with (137) or without (1) prostatectomy. Of the 154 patients randomized to the surgery alone group 144 underwent pelvic node dissection with (138) or without (6) prostatectomy. RESULTS There was no statistically significant difference between the 2 groups in operating time, blood loss, need for transfusion, postoperative morbidity or length of hospital stay. There were 4 rectal and 2 ureteral injuries in the surgery alone group and none in the pretreatment group (p < 0.05). Patients who received androgen deprivation preoperatively had a significantly lower rate of capsule penetration (47% versus 78%, p < 0.001), positive surgical margins (18% versus 48%, p < 0.001) and tumor at the urethral margin (6% versus 17%, p < 0.01). CONCLUSIONS Long-term followup data will be needed to determine whether there will be a lower incidence of biochemical relapse as determined by prostate specific antigen, local recurrence or metastasis, with an improvement in patient survival.
Right or Left Ventricular Pacing in Young Minipigs With Chronic Atrioventricular Block: Long-Term In Vivo Cardiac Performance, Morphology, Electrophysiology, and Cellular Biology

Background— Left ventricular (LV) dyssynchrony may occur as a result of right ventricular (RV) pacing and is a known risk factor for the development of heart failure. In children with complete atrioventricular block, pacing-induced dyssynchrony lasting for decades might be especially deleterious for LV function. To determine the hemodynamic and ultrastructural remodeling after either RV free wall or LV apical pacing, we used a chronic minipig model. Methods and Results— Fourteen piglets 8 weeks of age underwent atrioventricular node ablation and were paced from either the RV free wall or the LV apex at 120 bpm for 1 year (7 age-matched minipigs served as controls with spontaneous heart rates of 104±5 bpm). Echocardiographic examinations, pressure-volume loops, patch-clamp investigations, and examinations of connexin43, calcium-handling proteins, and histomorphology were carried out. RV free wall–paced minipigs exhibited significantly more LV dyssynchrony than LV apex–paced animals, which was accompanied by worsening of LV function (maximum LV mechanical delay/LV ejection fraction: RV free wall pacing, 154±36 ms/28±3%, LV apical pacing, 52±19 ms/45±2%, control 47±14 ms/62±1%; P=0.0001). At the cellular level, both pacemaker groups exhibited a significant reduction in L-type calcium and peak sodium current, shortening of action potential duration and amplitude, increased cell capacity, and alterations in the calcium-handling proteins that were similar for RV free wall– and LV apex–paced animals. Conclusions— The observed molecular remodeling seemed to be more dependent on heart rate than on dyssynchrony. LV apical pacing is associated with less dyssynchrony, a more physiological LV contraction pattern, and preserved LV function as opposed to RV free wall pacing.
[Traumatic life events and problem solving skills in psychiatric outpatients: relationships with suicidal behavior].

OBJECTIVE To investigate the prevalence and the relationship of traumatic life events and problem solving skills to suicidal behavior in a group of psychiatric outpatients. METHOD The study was conducted with 121 (57 % women) psychiatric outpatients. Patients who accepted participation filled in a questionnaire that contained questions about socio-demographics, clinical features, suicidal behavior, traumatic life-events, and a problem-solving inventory. Data were analyzed by means of t-tests, chi-square tests, one-way analysis of variance and multiple logistic regression analyses. RESULTS Of the patients, 43.2 % (47.8 % women) reported having thought and 28.7 % (29.7 % women) reported having attempted suicide. 75.4 % of the sample experienced at least one traumatic life-event. On average, a person experienced approximately 2 (SD = 1.7) traumatic events. Two logistic regression analyses showed that inefficient problem solving was an independent predictor of both suicidal thoughts and attempts. Number of traumatic events appeared to be an independent predictor of suicidal attempts after the problem solving skills. In accordance with diathesis-stress model, both suicidal thoughts and attempts were found to be most frequent among persons with inefficient problem solving skills and who were also exposed to a large amount of traumatic life events. CONCLUSION Suicidal behavior and traumatic life events are common among psychiatric patients. Findings indicate that inefficient problem solving may be an independent predictor of both suicidal ideation and attempts. Suicidal behavior, traumatic life-events and problem solving skills should be addressed during psychiatric assessment. Study findings imply that problem solving therapy may be an important approach that can be used for the treatment of psychiatric patients exhibiting suicidal behavior.
Three Third-Grade Teachers' Gender-Related Beliefs and Behavior

The purpose of this study was to investigate the ways in which 3 third-grade teachers diminished or contributed to the gender-differentiated schooling experiences of girls and boys by comparing the teachers' gender beliefs with what actually happened in their classrooms. The teachers, from a midwestern elementary school, were observed and interviewed during the 15-week data collection period. Data were analyzed using the constant-comparison method. Results indicated that the teachers worked from a gender-blind position, meaning they believed they did not take students' gender into account when teaching. However, the teachers' beliefs did not match their practices.