Miracle-dz/UniFAR-FTUs huggingface dataset:
Multifactorial Experimental Design to Optimize the Anti‐Inflammatory and Proangiogenic Potential of Mesenchymal Stem Cell Spheroids

Mesenchymal stem cell therapies promote wound healing by manipulating the local environment to enhance the function of host cells. Aggregation of mesenchymal stem cells (MSCs) into three‐dimensional spheroids increases cell survival and augments their anti‐inflammatory and proangiogenic potential, yet there is no consensus on the preferred conditions for maximizing spheroid function in this application. The objective of this study was to optimize conditions for forming MSC spheroids that simultaneously enhance their anti‐inflammatory and proangiogenic nature. We applied a design of experiments (DOE) approach to determine the interaction between three input variables (number of cells per spheroid, oxygen tension, and inflammatory stimulus) on MSC spheroids by quantifying secretion of prostaglandin E2 (PGE2) and vascular endothelial growth factor (VEGF), two potent molecules in the MSC secretome. DOE results revealed that MSC spheroids formed with 40,000 cells per spheroid in 1% oxygen with an inflammatory stimulus (Spheroid 1) would exhibit enhanced PGE2 and VEGF production versus those formed with 10,000 cells per spheroid in 21% oxygen with no inflammatory stimulus (Spheroid 2). Compared to Spheroid 2, Spheroid 1 produced fivefold more PGE2 and fourfold more VEGF, providing the opportunity to simultaneously upregulate the secretion of these factors from the same spheroid. The spheroids induced macrophage polarization, sprout formation with endothelial cells, and keratinocyte migration in a human skin equivalent model—demonstrating efficacy on three key cell types that are dysfunctional in chronic non‐healing wounds. We conclude that DOE‐based analysis effectively identifies optimal culture conditions to enhance the anti‐inflammatory and proangiogenic potential of MSC spheroids. Stem Cells 2017;35:1493–1504
Paradigmatic effects in auditory word recognition: The case of alternating voice in Dutch

Two lexical decision experiments addressed the role of paradigmatic effects in auditory word recognition. Experiment 1 showed that listeners classified a form with an incorrectly voiced final obstruent more readily as a word if the obstruent is realised as voiced in other forms of that word's morphological paradigm. Moreover, if such was the case, the exact probability of paradigmatic voicing emerged as a significant predictor of the response latencies. A greater probability of voicing correlated with longer response latencies for words correctly realised with voiceless final obstruents. A similar effect of this probability was observed in Experiment 2 for words with completely voiceless or weakly voiced (incompletely neutralised) final obstruents. These data demonstrate the relevance of paradigmatically related complex words for the processing of morphologically simple words in auditory word recognition.
Simultaneous Lp-stabilization and internal stabilization for linear singular systems subject to input saturation

The simultaneous Lp-stabilization and internal stabilization for continuous-time linear singular systems subject to input saturation are considered. A scheduled low-and high-gain state feedback control law is proposed, which relies on the asymptotical property of a class of parametric generalized algebraic Riccati equations. When applied to the singular system with input saturation and external disturbance, finite-gain Lp-stabilization and global internal stabilization are obtained simultaneously. The result reduces to the existing results for non-singular linear systems when the linear singular system reduces to a non-singular system. Furthermore, the result also extends the semi-global stabilization results for singular systems with saturation in the absence of the external disturbance.
Smart macroencapsulated resin/wax composite for energy conservation in the built environment

This study reports the results of experimental and numerical investigations on the thermophysical properties and the process of melting of a phase-change composite material. The proposed phase-change composite material based on epoxy resin with spherical shape paraffin wax (RT27) was used as a new thermal storage system. Thermal characterization was performed using a transient guarded hot plate technique. The results revealed the importance of thermal storage by latent heat. The numerical analysis is realized using numerical COMSOL® Multiphysics 4.3b. The effect of various parameters of the numerical solution on the results is examined: in particular, the term describing the mushy zone in the momentum equation and the influence of temperature melting range. The findings of the experimental investigation compare favorably with the numerical results.
Genetic and environmental sources of covariance among internal reproductive traits in the yellow dung fly

Substantial inter‐ and intraspecific variation is found in reproductive traits, but the evolutionary implications of this variation remain unclear. One hypothesis is that natural selection favours female reproductive morphology that allows females to control mating and fertilization and that diverse male reproductive traits arise as counter adaptations to subvert this control. Such co‐evolution predicts the establishment of genetic correlations between male and female reproductive traits that closely interact during mating. Therefore, we measured phenotypic and genetic correlations between male and female reproductive tract characteristics in the yellow dung fly, Scathophaga stercoraria (Diptera: Scathophagidae), using a nested half‐sib breeding experiment. We found significant heritabilities for the size of most reproductive tract traits investigated in both females (spermathecae and their ducts, accessory glands and their ducts) and males (testis size but not sperm length). Within the sexes, phenotypic and genetic correlations were mostly nil or positive, suggesting functional integration of or condition‐dependent investment in internal reproductive traits. Negative intrasexual genetic correlations, potentially suggestive of resource allocation trade‐offs, were not evident. Intersexual genetic correlations were mostly positive, reflecting expected allometries between male and female morphologies. Most interestingly, testis size correlated positively with female accessory gland size and duct length, potentially indicative of a co‐evolutionary arms race. We discuss these and alternative explanations for these patterns of genetic covariance.
Discovery and development of tramadol for the treatment of pain

ABSTRACT Introduction: Tramadol is an opioid drug that, unlike classic opioids, also modulates the monoaminergic system by inhibiting noradrenergic and serotoninergic reuptake. For this reason, tramadol is considered an atypical opioid. These special pharmacological characteristics have made tramadol one of the most commonly prescribed analgesic drugs to treat moderate to severe pain. Areas covered: The aim of this review is to provide a historical description of the biochemistry, pharmacokinetics and particularly, the mechanisms of action of tramadol. In addition, a summary is offered of the analgesic effects of tramadol in a variety of animal models of acute and chronic pain. Finally, clinical studies that demonstrate the efficacy and safety of tramadol in the treatment of pain are also assessed. Expert opinion: The discovery that tramadol combines opioid and monoaminergic effects represented a milestone in the evolution of pain treatment. Given its ‘mild effect’ on opioid receptors, tramadol induces fewer side effects than classic opioids. Tramadol produces satisfactory analgesia against various types of pain and it is currently approved for the treatment of moderate to severe pain. Thus, the combination of monoamine and opioid mechanisms opens new avenues for the design of innovative analgesics.
Cigarette Butt Decomposition and Associated Chemical Changes Assessed by 13C CPMAS NMR

Cigarette butts (CBs) are the most common type of litter on earth, with an estimated 4.5 trillion discarded annually. Apart from being unsightly, CBs pose a serious threat to living organisms and ecosystem health when discarded in the environment because they are toxic to microbes, insects, fish and mammals. In spite of the CB toxic hazard, no studies have addressed the effects of environmental conditions on CB decomposition rate. In this study we investigate the interactive effects of substrate fertility and N transfer dynamics on CB decomposition rate and carbon quality changes. We carried out an experiment using smoked CBs and wood sticks, used as a slow decomposing standard organic substrate, incubated in both laboratory and field conditions for two years. CB carbon quality changes during decomposition was assessed by 13C CPMAS NMR. Our experiment confirmed the low degradation rate of CBs which, on average, lost only 37.8% of their initial mass after two years of decomposition. Although a net N transfer occurred from soil to CBs, contrary to our hypothesis, mass loss in the medium-term (two years) was unaffected by N availability in the surrounding substrate. The opposite held for wood sticks, in agreement with the model that N-rich substrates promote the decomposition of other N-poor natural organic materials with a high C/N ratio. As regards CB chemical quality, after two years of decomposition 13C NMR spectroscopy highlighted very small changes in C quality that are likely to reflect a limited microbial attack.
A new high‐precision 40Ar/39Ar age for the Rochechouart impact structure: At least 5 Ma older than the Triassic–Jurassic boundary

The Rochechourt impact structure in south‐central France, with maximum diameter of 40–50 km, has previously been dated to within 1% uncertainty of the Triassic–Jurassic boundary, at which time ~30% of global genera became extinct. To evaluate the temporal relationship between the impact and the Triassic–Jurassic boundary at high precision, we have re‐examined the structure's age using multicollector ARGUS‐V 40Ar/39Ar mass spectrometry. Results from four aliquots of impact melt are highly reproducible, and yield an age of 206.92 ± 0.20/0.32 Ma (2σ, full analytical/external uncertainties). Thus, the Rochechouart impact structure predates the Triassic–Jurassic boundary by 5.6 ± 0.4 Ma and so is not temporally linked to the mass extinction. Rochechouart has formerly been proposed to be part of a multiple impact event, but when compared with new ages from the other purported “paired” structures, the results provide no evidence for synchronous impacts in the Late Triassic. The widespread Central Atlantic Magmatic Province flood basalts remain the most likely cause of the Triassic–Jurassic mass extinction.
Vibration damping in manipulation of deformable linear objects using sliding mode control

Manipulation of deformable linear objects (DLOs) such as hoses, wires and so on is common in daily life and some manufacturing applications. Whereas, the DLOs are more challengeable to handle than rigid objects, as the uncertainty resulting from oscillation at the end of DLOs may cause failure in the operation. In this paper, based on the dynamic model of a DLO in our recent work, we proposed a position-based control strategy for eliminating the vibration at the end of DLOs. First, the simplified linear model of a DLO can be obtained by local linearization about the equilibrium. Then, by using Schur decomposition of matrices and linear transform of variables, the couplings between states are eliminated, and the actuated and underactuated parts of the DLO system are separated. Finally, based on the simplified linear model with a position constraint condition, a control strategy using sliding mode control (SMC) with exponential approach law is illustrated. The proposed control strategy is verified by numerical simulations. The simulation results show that the proposed method can damp the vibration effectively.
Allowing for non-adherence to treatment in a randomized controlled trial of two antidepressants (citalopram versus reboxetine): an example from the GENPOD trial

Background Meta-analyses suggest that reboxetine may be less effective than other antidepressants. Such comparisons may be biased by lower adherence to reboxetine and subsequent handling of missing outcome data. This study illustrates how to adjust for differential non-adherence and hence derive an unbiased estimate of the efficacy of reboxetine compared with citalopram in primary care patients with depression. Method A structural mean modelling (SMM) approach was used to generate adherence-adjusted estimates of the efficacy of reboxetine compared with citalopram using GENetic and clinical Predictors Of treatment response in Depression (GENPOD) trial data. Intention-to-treat (ITT) analyses were performed to compare estimates of effectiveness with results from previous meta-analyses. Results At 6 weeks, 92% of those randomized to citalopram were still taking their medication, compared with 72% of those randomized to reboxetine. In ITT analysis, there was only weak evidence that those on reboxetine had a slightly worse outcome than those on citalopram [adjusted difference in mean Beck Depression Inventory (BDI) scores: 1.19, 95% confidence interval (CI) –0.52 to 2.90, p = 0.17]. There was no evidence of a difference in efficacy when differential non-adherence was accounted for using the SMM approach for mean BDI (–0.29, 95% CI –3.04 to 2.46, p = 0.84) or the other mental health outcomes. Conclusions There was no evidence of a difference in the efficacy of reboxetine and citalopram when these drugs are taken and tolerated by depressed patients. The SMM approach can be implemented in standard statistical software to adjust for differential non-adherence and generate unbiased estimates of treatment efficacy for comparisons of two (or more) active interventions.
Ecological mechanisms underlying soil bacterial responses to rainfall along a steep natural precipitation gradient

ABSTRACT Changes in the structure and function of soil microbial communities can drive substantial ecosystem feedbacks to altered precipitation. However, the ecological mechanisms underlying community responses to environmental change are not well understood. We used an 18‐month soil reciprocal transplant experiment along a steep precipitation gradient to quantify how changes in rainfall affected bacterial community structure. We also conducted an enhanced dispersal treatment to ask whether higher immigration rates of taxa from the surrounding environment would accelerate community responses to climate change. Finally, we addressed how the composition of soil bacteria communities was related to the functional response of soil respiration to moisture in these treatments. Bacterial community structure (OTU abundance) and function (respiration rates) changed little in response to manipulation of either rainfall environment or dispersal rates. Although most bacteria were ecological generalists, a subset of specialist taxa, over 40% of which were Actinobacteria, tended to be more abundant in the rainfall environment that matched their original conditions. Bacteria community composition was an important predictor of the respiration response to moisture. Thus, the high compositional resistance of microbial communities dictated respiration responses to altered rainfall in this system.
Slow Gait Speed and Cardiac Rehabilitation Participation in Older Adults After Acute Myocardial Infarction

Background Lack of participation in cardiac rehabilitation (CR) and slow gait speed have both been associated with poor long‐term outcomes in older adults after acute myocardial infarction (AMI). Whether the effect of CR participation on outcomes after AMI differs by gait speed is unknown. Methods and Results We examined the association between gait speed and CR participation at 1 month after discharge after AMI, and death and disability at 1 year, in 329 patients aged ≥65 years enrolled in the TRIUMPH (Translational Research Investigating Underlying Disparities in Recovery From Acute Myocardial Infarction: Patients' Health Status) registry. Among these patients, 177 (53.7%) had slow gait speed (<0.8 m/s) and 109 (33.1%) participated in CR. Patients with slow gait speed were less likely to participate in CR compared with patients with normal gait speed (27.1% versus 40.1%; P=0.012). In unadjusted analysis, CR participants with normal gait speed had the lowest rate of death or disability at 1 year (9.3%), compared with those with slow gait speed and no CR participation (43.2%). After adjustment for cardiovascular risk factors and cognitive impairment, both slow gait speed (odds ratio, 2.30; 95% confidence interval, 1.30–4.06) and non‐CR participation (odds ratio, 2.34; 95 confidence interval, 1.22–4.48) were independently associated with death or disability at 1 year. The effect of CR on the primary outcome did not differ by gait speed (P=0.70). Conclusions CR participation is associated with reduced risk for death or disability after AMI. The beneficial effect of CR participation does not differ by gait speed, suggesting that slow gait speed alone should not preclude referral to CR for older adults after AMI.
Spatial-temporal population dynamics across species range: from centre to margin

Understanding the boundaries of species' ranges and the variations in population dynamics from the centre to margin of a species' range is critical. This study simulated spatial-temporal patterns of birth and death rates and migration across a species' range in different seasons. Our results demonstrated the importance of dispersal and migration in altering birth and death rates, balancing source and sink habitats, and governing expansion or contraction of species' ranges in changing environments. We also showed that the multiple equilibria of metapopulations across a species' range could be easily broken following climatic changes or physical disturbances either local or regional. Although we refer to our models as describing the population dynamics across whole species' range, they should also apply to small-scale habitats (metapopulations) in which species abundance follows a humped pattern or to any ecosystem or landscape where strong central-marginal (C-M) environmental gradients exist. Conservation of both central and marginal populations would therefore be equally important considerations in making management decisions.
Diversity of flies (Diptera: Tephritidae and Lonchaeidae) in organic citrus orchards in the vale do Rio Caí, Rio Grande do Sul, southern Brazil.

This study was carried out in the Counties of Montenegro and Pareci Novo located in the region of the Vale do Rio Cai, Rio Grande do Sul, Southern Brazil, aiming to determine the fruit fly species of Tephritidae and Lonchaeidae that occur in organic orchards of sweet orange [Citrus sinensis (L.) Osb. ] cultivar Céu, and Murcott tangor (Citrus reticulata Blanco x C. sinensis), during the fruit ripening stages in 2003 and 2004. Eight McPhail traps baited with integral grape juice diluted to 25% were installed in four orchards, two in each citrus species. The traps were checked weekly, when the baits were changed, the flies separated and preserved in 70% ethyl alcohol. Fruits were also sampled from the orchards, placed in containers with damp soil and closed with a mesh. The tephritid flies represented 86.2% of all captured flies in the four orchards during both years. Five Tephritidae species were captured from traps: Anastrepha fraterculus (Wiedemann), Anastrepha grandis (Macquart), Anastrepha pseudoparallela (Loew), Anastrepha dissimilis Stone and Ceratitis capitata (Wiedemann). The captured species of Lonchaeidae were: Neosilba zadolicha McAlpine & Steyskal, Neosilba n.sp. 3, Neosilba sp. and Lonchaea sp. Anastrepha fraterculus was found in 99% of the fruit samples, both in 'Céu' orange and 'Murcott' tangor, and Neosilba n.sp. 3 were only obtained from 'Murcott' tangor fruits.
Possible Involvement of the Rat Hypothalamo‐Neurohypophysial/‐Spinal Oxytocinergic Pathways in Acute Nociceptive Responses

Oxytocin (OXT)‐containing neurosecretory cells in the parvocellular divisions of the paraventricular nucleus (PVN), which project to the medulla and spinal cord, are involved in various physiological functions, such as sensory modulation and autonomic processes. In the present study, we examined OXT expression in the hypothalamo‐spinal pathway, as well as the hypothalamo‐neurohypophysial system, which includes the magnocellular neurosecretory cells in the PVN and the supraoptic nucleus (SON), after s.c. injection of saline or formalin into the hindpaws of transgenic rats that express the OXT and monomeric red fluorescent protein 1 (mRFP1) fusion gene. (i) The numbers of OXT‐mRFP1 neurones that expressed Fos‐like immunoreactivity (‐IR) and OXT‐mRFP1 intensity were increased significantly in the magnocellular/parvocellular PVN and SON after s.c. injection of formalin. (ii) OXT‐mRFP1 neurones in the anterior parvocellular PVN, which may project to the dorsal horn of the spinal cord, were activated by s.c. injection of formalin, as indicated by a significant increases of Fos‐IR and mRFP1 intensity intensity. (iii) Formalin injection caused a significant transient increase in plasma OXT. (iv) OXT, mRFP1 and corticotrophin‐releasing hormone mRNAs in the PVN were significantly increased after s.c. injection of formalin. (v) An intrathecal injection of OXT‐saporin induced hypersensitivity in conscious rats. Taken together, these results suggest that the hypothalamo‐neurohypophysial/‐spinal OXTergic pathways may be involved in acute nociceptive responses in rats.
Open Packet Processor: a programmable architecture for wire speed platform-independent stateful in-network processing

This paper aims at contributing to the ongoing debate on how to bring programmability of stateful packet processing tasks inside the network switches, while retaining platform independency. Our proposed approach, named "Open Packet Processor" (OPP), shows the viability (via an hardware prototype relying on commodity HW technologies and operating in a strictly bounded number of clock cycles) of eXtended Finite State Machines (XFSM) as low-level data plane programming abstraction. With the help of examples, including a token bucket and a C4.5 traffic classifier based on a binary tree, we show the ability of OPP to support stateful operation and flow-level feature tracking. Platform independence is accomplished by decoupling the implementation of hardware primitives (registries, conditions, update instructions, forwarding actions, matching facilities) from their usage by an application formally described via an abstract XFSM. We finally discuss limitations and extensions.
The Effect of Yarn Hairiness on Air Drag in Ring Spinning

Air drag on yarn and package surfaces affects yarn tension, which in turn affects energy consumption and ends-down in ring spinning. This study investigated the effects of yarn hairiness on air drag in ring spinning. Theoretical models of skin friction coefficient on the surface of rotating yarn packages were developed. The predicted results were verified with experimental data obtained from cotton and wool yarns. The results show that hairiness increases the air drag by about one-quarter and one-third for the rotating cotton and wool yarn packages, respectively. In addition, yarn hairiness increases the air drag by about one-tenth on a ballooning cotton yarn.