Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison Between Hydrogen Clearance and Microsphere Techniquefor rCBF Measurement

Regional cerebral blood flow was measured repeatedly in anesthetized and immobilized cats under various experimental conditions by recording the clearance of inhaled hydrogen with inserted platinum electrodes and by recording the distribution of 15 n microspheres labeled with 3 different radioisotopes. The values from both methods in normal cortical tissue were comparable (75.7 ± 23.5 ml/100 g min for H2-clearance; 67.6 ± 26.2 ml/100 g min for microsphere technique), but were below those recorded in awake cats. With both methods the values could be reliably reproduced (correlation coefficient between measurements: 0.903 for H2-clearance, 0.754 for microsphere technique). During ischemia induced by temporary occlusion of the middle cerebral artery the microsphere technique usually yielded higher flow values than the H2-clearance, and did not indicate severe ischemia in 6 out of 20 instances. After restoration of flow, hyperperfusion was observed by the microsphere technique in 2 cases only while H2-clearance indicated hyperemia in 6 instances. This limited comparability between the 2 methods was also expressed in a low correlation coefficient (0.486) calculated from 139 flow values obtained simultaneously with both methods. The discrepancy between the methods under pathological conditions might be due mainly to the different recording volumes: while Pt-electrodes record H2-clearance from a few mm' or less, tissue samples of 300-700 mg were necessary for the microsphere technique and inhomogeneities of flow may thereby escape detection. The technique for measuring cerebral blood flow in an experimental setup should be selected according to the requirements of the study and according to the limitations of the various methods. Stroke, Vol 12, No 2, 1981
Inhibition of Glycogen Synthase Kinase-3 Ameliorates β-Amyloid Pathology and Restores Lysosomal Acidification and Mammalian Target of Rapamycin Activity in the Alzheimer Disease Mouse Model

Background: The mechanisms behind the contribution of GSK-3 to Alzheimer disease pathogenesis remain elusive. Results: A GSK-3 inhibitor reduced Aβ pathology and ameliorated cognitive decline in an Alzheimer disease mouse model. GSK-3 impairs lysosomal acidification and impacts mTOR activity. Conclusion: Inhibition of GSK-3 reverses Alzheimer disease pathogenesis via restoration of lysosomal acidification and reactivation of mTOR. Significance: We identified novel mechanisms linking GSK-3 with Aβ pathology. Accumulation of β-amyloid (Aβ) deposits is a primary pathological feature of Alzheimer disease that is correlated with neurotoxicity and cognitive decline. The role of glycogen synthase kinase-3 (GSK-3) in Alzheimer disease pathogenesis has been debated. To study the role of GSK-3 in Aβ pathology, we used 5XFAD mice co-expressing mutated amyloid precursor protein and presenilin-1 that develop massive cerebral Aβ loads. Both GSK-3 isozymes (α/β) were hyperactive in this model. Nasal treatment of 5XFAD mice with a novel substrate competitive GSK-3 inhibitor, L803-mts, reduced Aβ deposits and ameliorated cognitive deficits. Analyses of 5XFAD hemi-brain samples indicated that L803-mts restored the activity of mammalian target of rapamycin (mTOR) and inhibited autophagy. Lysosomal acidification was impaired in the 5XFAD brains as indicated by reduced cathepsin D activity and decreased N-glycoyslation of the vacuolar ATPase subunit V0a1, a modification required for lysosomal acidification. Treatment with L803-mts restored lysosomal acidification in 5XFAD brains. Studies in SH-SY5Y cells confirmed that GSK-3α and GSK-3β impair lysosomal acidification and that treatment with L803-mts enhanced the acidic lysosomal pool as demonstrated in LysoTracker Red-stained cells. Furthermore, L803-mts restored impaired lysosomal acidification caused by dysfunctional presenilin-1. We provide evidence that mTOR is a target activated by GSK-3 but inhibited by impaired lysosomal acidification and elevation in amyloid precursor protein/Aβ loads. Taken together, our data indicate that GSK-3 is a player in Aβ pathology. Inhibition of GSK-3 restores lysosomal acidification that in turn enables clearance of Aβ burdens and reactivation of mTOR. These changes facilitate amelioration in cognitive function.
Results of a Wind Turbine FDI Competition

In this paper some newly published methods for fault detection and isolation developed for a wind turbine benchmark model are tested, compared and evaluated. These methods have been presented as a part of an international competition. The tested methods cover different types of fault detection and isolation methods, which include support vector machines, observer based methods, and auto generated methods. All of these methods show interesting potentials for usage in the wind turbine application, but all with different strong and weak sides in relation to the requirements specified in the proposed benchmark model.
Therapeutic B cell depletion impairs adaptive and autoreactive CD4+ T cell activation in mice

CD20 antibody depletion of B lymphocytes effectively ameliorates multiple T cell-mediated autoimmune diseases through mechanisms that remain unclear. To address this, a mouse CD20 antibody that depletes >95% of mature B cells in mice with otherwise intact immune systems was used to assess the role of B cells in CD4+ and CD8+ T cell activation and expansion in vivo. B cell depletion had no direct effect on T cell subsets or the activation status of CD4+ and CD8+ T cells in naive mice. However, B cell depletion impaired CD4+ T cell activation and clonal expansion in response to protein antigens and pathogen challenge, whereas CD8+ T cell activation was not affected. In vivo dendritic cell ablation, along with CD20 immunotherapy, revealed that optimal antigen-specific CD4+ T cell priming required both B cells and dendritic cells. Most importantly, B cell depletion inhibited antigen-specific CD4+ T cell expansion in both collagen-induced arthritis and autoimmune diabetes mouse models. These results provide direct evidence that B cells contribute to T cell activation and expansion in vivo and offer insights into the mechanism of action for B cell depletion therapy in the treatment of autoimmunity.
Differences in Action Style Recognition in Children with Autism Spectrum Disorders

Vitality form is a term, originally introduced by Stern (2010), to describe “how” an action is performed. The capacity to perceive the vitality form of others’ actions is a fundamental element of social interactions and a basic way of relating to and understanding others’ behaviors. Although vitality forms characterize all human interactions, few studies have addressed their role in social and communicative disorders such as autism. The aim of the present study is to evaluate the ability to recognize different vitality forms during the observation of different motor actions in a group of children with autism spectrum disorders (ASD) compared to typically developing controls (TD). Results show a significant difference between children with ASD and TD in vitality forms recognition. This finding sheds new light on how children with ASD understand others’ actions providing new ideas on overall social understanding as well as useful insights for professionals and caregivers alike.
Studies on Neuroprotective and Regenerative Effects of GDNF in a Partial Lesion Model of Parkinson's Disease

Intrastriatal 6-hydroxydopamine injections in rats induce partial lesions of the nigrostriatal dopamine (DA) system which are accompanied by a delayed and protracted degeneration of DA neurons within the substantia nigra. By careful selection of the dose and placement of the toxin it is possible to obtain reproducible and regionally defined partial lesions which are well correlated with stable functional deficits, not only in drug-induced behaviors but also in spontaneous motoric and sensorimotoric function, which are analogous to the symptoms seen in patients during early stages of Parkinson's disease. The intrastriatal partial lesion model has proved to be particularly useful for studies on the mechanisms of action of neurotrophic factors since it offers opportunities to investigate both protection of degenerating DA neurons during the acute phases after the lesion and stimulation of regeneration and functional recovery during the chronic phase of the postlesion period when a subset of the spared nigral DA neurons persist in an atrophic and dysfunctional state. In the in vivo experiments performed in this model glial cell line-derived neurotrophic factor (GDNF) has been shown to exert neurotrophic effects both at the level of the cell bodies in the substantia nigra and at the level of the axon terminals in the striatum. Intrastriatal administration of GDNF appears to be a particularly effective site for induction of axonal sprouting and regeneration accompanied by recovery of spontaneous sensorimotor behaviors in the chronically lesioned nigrostriatal dopamine system.
Circularly establishing feature correspondences and motion estimation for object tracking via multivisit of Kalman filtering

The validity of feature correspondences plays an important role for feature-correspondence based motion estimation, which leads to the final goal of object tracking. Though different data association methods have been proposed, the problem of feature correspondence is, in general, ill-posed due to either the presences of multiple candidates within search regions or no candidates because of occlusion or other factors. Our research is inspired by how we evaluate the effectiveness of the feature correspondence and how the evaluation will affect motion estimation. The evaluation of template based feature correspondence is achieved by considering the feedback of the latest motion estimation from first visit of Kalman Filtering. Then motion estimation and feature correspondence are re-processed based on evaluation result, which constitutes the second and third visit of Kalman filtering. What makes our work different from others is also that instead of restricting the semantic object tracking in 2D domain, our framework is formulated to recover the 3D depth values of selected features during motion estimation process.
Timekeeping in the memory system: predicting and optimizing memory behavior

This paper offers afresh perspective on the problem of predicting and optimizing memory behavior. We show quantitatively the extent to which detailed timing characteristics of past memory reference events are strongly predictive of future program reference behavior. We propose a family of timekeeping techniques that optimize behavior based on observations about particular cache time durations, such as the cache access interval or the cache dead time. Timekeeping techniques can be used to build small, simple, and high-accuracy (often 90% or more) predictors for identifying conflict misses, for predicting dead blocks, and even for estimating the time at which the next reference to a cache frame will occur and the address that will be accessed. Based on these predictors, we demonstrate two new and complementary time-based hardware structures: (1) a time-based victim cache that improves performance by only storing conflict miss lines with likely reuse, and (2) a time-based prefetching technique that hones in on the right address to prefetch, and the right time to schedule the prefetch.
Energy-limited wireless networking with directional antennas: the case of session-based multicasting

We consider ad hoc wireless networks that use directional antennas and have limited energy resources. The performance objectives of such networks depend largely on the application. However, a robust performance measure is the total traffic volume that the network can deliver when all nodes are equipped with a finite and non-renewable amount of energy. We show that the network's lifetime can be extended significantly by incorporating a simple measure of a node's residual energy into the node's cost function. To explore quantitatively the advantage offered by the use of directional antennas over the case of omnidirectional antennas, we consider the case of connection-oriented multicast traffic. Building upon our prior work on multicasting algorithms, we introduce two protocols that exploit the use of directional antennas and evaluate their performance. We observe significant improvement with respect to the omnidirectional case.
Oral health of Hong Kong children: a historical and epidemiological perspective.

OBJECTIVE To provide a historical and epidemiological overview of the oral health of Hong Kong children. METHODS Literature published before 2014 related to the oral health of Hong Kong children, supplemented with information accessed from government-archived oral health reports, was sourced using electronic databases and hand searches. Dental caries experience, periodontal health, enamel defects, and malocclusion of Hong Kong children were reviewed. RESULTS A decline in the prevalence and extent of dental caries was observed among Hong Kong schoolchildren and adolescents after the 1960s. Among preschool children, however, dental caries remains common and the extent appears to have increased. The periodontal health of Hong Kong children remains unsatisfactory. Recently, enamel defects/dental fluorosis have considerably reduced. Information about malocclusion in Hong Kong children is limited. CONCLUSIONS Since the 1960s, following public health policies, health promotion activities, and the introduction of a School Dental Care Service, improvements in the oral health of schoolchildren are evident. Nonetheless, the oral health of preschool children remains a concern. Policies and practices to improve the oral health of preschool children in Hong Kong are required.
Implications of loyalty program membership and service experiences for customer retention and value

Companies that offer loyalty reward programs believe that their programs have a long-run positive effect on customer evaluations and behavior. However, if loyalty rewards programs increase relationship durations and usage levels, customers will be increasingly exposed to the complete spectrum of service experiences, including experiences that may cause customers to switch to another service provider. Using cross-sectional, time-series data from a worldwide financial services company that offers a loyalty reward program, this article investigates the conditions under which a loyalty rewards program will have a positive effect on customer evaluations, behavior, and repeat purchase intentions. The results show that members in the loyalty reward program overlook or discount negative evaluations of the company vis-à-vis competion. One possible reason could be that members of the loyalty rewards program perceive that they are getting better quality and service for their price or, in other words, “good value. ”
Sex difference in age of onset of schizophrenia: findings from a community‐based study in India

This study examined the sex difference in age of onset of schizophrenia in a community sample. Community-level health workers identified patients with symptoms of schizophrenia living in the community in a defined geographical area in South India. Two hundred and nine of them were diagnosed as hav-ing schizophrenia according to ICD-10 criteria by a team of psychiatrists. The age of onset of schizophrenia was assessed using the Interview for Retro-spective Assessment of Onset of Schizophrenia (IRAOS). The mean age of onset of schizophrenia did not significantly differ between males (29.2+/-8.8 years) and females (30.8+/-11.4 years) (t = 1.12; p = 0.27). Among those with an age of onset </=33 years, females had a significantly earlier onset; among those with an age of onset >33 years, females had a significantly later onset. The results from this community-based study confirm the previous findings in hospital-based patients in Asia. There is a need to revise the description of schizophrenia in the classificatory systems, keeping in view the regional varia-tions in the age of onset of the disorder.
The O-Antigen Capsule of Salmonella enterica Serovar Typhimurium Facilitates Serum Resistance and Surface Expression of FliC

ABSTRACT Group IV polysaccharide capsules are common in enteric bacteria and have more recently been described in nontyphoidal Salmonella species. Such capsules are known as O-antigen (O-Ag) capsules, due to their high degree of similarity to the O-Ag of the lipopolysaccharide (LPSO-Ag). Capsular polysaccharides are known virulence factors of many bacterial pathogens, facilitating evasion of immune recognition and systemic dissemination within the host. Previous studies on the O-Ag capsule of salmonellae have focused primarily on its role in bacterial surface attachment and chronic infection; however, the potential effects of the O-Ag capsule on acute pathogenesis have yet to be investigated. While much of the in vivo innate immune resistance of Salmonella enterica serovar Typhimurium is attributed to the high-molecular-weight LPS, we hypothesized that the O-Ag capsule may enhance this resistance by diminishing surface expression of pathogen-associated molecular patterns, such as flagella, and increasing resistance to host immune molecules. To test this hypothesis, O-Ag capsule-deficient mutants were constructed, and the loss of O-Ag capsular surface expression was confirmed through microscopy and immunoblotting. Loss of O-Ag capsule production did not alter bacterial growth or production of LPS. Western blot analysis and confocal microscopy revealed that O-Ag capsule-deficient mutants demonstrate reduced resistance to killing by human serum. Furthermore, O-Ag capsule-deficient mutants produced exclusively phase I flagellin (FliC). Although O-Ag capsule-deficient mutants did not exhibit reduced virulence in a murine model of acute infection, in vitro results indicate that the O-Ag capsule may function to modify the antigenic nature of the bacterial surface, warranting additional investigation of a potential role of the structure in pathogenesis.
Health Locus of Control and Self-Efficacy Beliefs in a Healthy Elderly Sample

Background. The elderly are the fastest growing segment of the population, and this will place additional burdens on the healthcare system. It is therefore valuable to study the elderly who have reached their later years relatively free of disease and in good health. Methods. Health locus of control, self-efficacy beliefs, and lifestyle behaviors were studied in a sample of 57 healthy elderly subjects. Subjects completed the Multidimensional Health Locus of Control Scale, Self-Efficacy Scale, and Healthstyle Self- Test for Seniors. Results. Most of the subjects were characterized by an internal health locus of control belief (91.2%), high generalized self-efficacy (57.9%), and good health behaviors. As hypothesized, positive relationships were found among these variables. Discussion. Individuals with an internal health locus of control and high generalized self-efficacy are more likely to benefit from a health education program than those with an external locus of control and low self-efficacy. Health educators can better serve their clients by evaluating these parameters when developing programs.
p40phox-Deficient Mice Exhibit Impaired Bacterial Clearance and Enhanced Pro-inflammatory Responses during Salmonella enterica serovar Typhimurium Infection

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major cause of acute gastroenteritis in humans. During infection, reactive oxygen species (ROS), generated from NADPH oxidase (a multisubunit enzyme complex), are required for pathogen killing upon phagocytosis and for regulating pro-inflammatory signaling in phagocytic cells. Mutations in subunits forming the NADPH complex may lead to enhanced susceptibility to infection and inflammatory disease. Compared to other NADPH oxidase subunits, the function of p40phox is relatively understudied, particularly in the context of intestinal bacterial infection. In this study, we utilized genetically engineered mice to determine the role of p40phox in the response to S. Typhimurium infection. We show that mice lacking p40phox are more susceptible to oral infection with S. Typhimurium, as demonstrated by significantly enhanced bacterial dissemination to spleen and liver, and development of exacerbated bacterial colitis. Moreover, we demonstrate that the increased infection and disease severity are correlated with markedly increased F4/80+ macrophage and Ly6G+ neutrophil infiltration in the infected tissues, coincident with significantly elevated pro-inflammatory cytokines (IL-1β and TNF-α) and chemoattractant molecules in the infected tissues. Functional analysis of macrophages and neutrophils further shows that p40phox deficiency impairs bacteria- or PMA-induced intracellular ROS production as well as intracellular killing of Salmonella. These observations indicate that the p40phox subunit of NADPH oxidase plays an essential role in suppressing intracellular multiplication of Salmonella in macrophages and in the regulation of both systemic and mucosal inflammatory responses to bacterial infection.
Cloned human teratoma cells differentiate into neuron-like cells and other cell types in retinoic acid.

Single cell clones were isolated from the human teratoma line, Tera-2. The cells of three of these clones were studied. The progressively growing cells were shown to be tumorigenic, and they were characterized by the lack of expression of beta 2-microglobulin and HLA-A,B,C determinants on the cell surface. The majority of the cells expressed Thy-1 antigen and a 90 X 10(3) molecular weight protein recognized by the monoclonal antibody F10.44. 2; between a third and half of the cells expressed the sugar specificities detected by the anti-SSEA-1 monoclonal antibody. In response to 5 X 10(-5) M-retinoic acid applied to cells in monolayer culture, the cells differentiated into a population of flat static cells arrested in the G1 phase of the cell cycle. A substantial proportion of these differentiated cells expressed beta 2-microglobulin and 43 X 10(3) molecular weight HLA-A,B,C polypeptides, Thy-1, SSEA-1 sugar determinants, and the 90 X 10(3) Mr protein recognized by F10.44. 2. The apparent molecular weight of fibronectin secreted by the cells decreased by about 5 X 10(3) Mr to 235 X 10(3) Mr after differentiation. The progressively growing cells lacked reactivity with reagents that mark cells in the nervous system. Following aggregation and retinoic acid treatment, neuron-like cells were formed. These cells reacted with reagents that also react with human neurons in culture: they reacted with tetanus toxin, the anti-neurofilament antibodies BF10 and RT97, the anti-ganglioside, GQ1c antibody F12 A2B5, and anti-Thy-1. The progressively growing cells of these Tera-2 clones are therefore capable of forming at least two types of cell: the flat cells in monolayer cultures and the neuron-like cells. None of the cell populations reacted with the monoclonal antibody against SSEA-3 and these cloned cells are therefore distinct from previous isolates from Tera-2.