Miracle-dz/UniFAR-FTUs huggingface dataset:
Mitogen‐Induced Interleukin 2 and Gamma Interferon Production by CD4+ and CD8+ Cells of Patients with Inflammatory Arthritides. A Comparison between Cells from Synovial Fluid and Peripheral Blood

The purpose of this study was to investigate interleukin 2 (IL‐2) and gamma interferon (IFN‐γ) production by purified CD4+ and CD8+ cells isolated from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis (RA) and other inflammatory arthritides (non‐RA). CD4+ and CD8+ cells were selected positively by immunomagnetic separation. Supernatants of unstimulated CD4+ and CD8+ cells from both compartments did not contain any delectable IL‐2 or IFN‐γ, while supernatants of CD4+ and CDSI cells stimulated with phytohaemagglutinin and irradiated Raji cells mostly contained both cytokines. In vitro stimulated SF CD4+ cells gave supernatants with significantly less IL‐2 than supernatants from PBCD4+ cells, while in vitro stimulated SF CD4+ ‐cell supernatants contained significantly more IFN‐γ. SF CD4+ ‐cell supernatants contained significantly more IL‐2 than the parallel CD8+ supernatants, while there was no significant difference with regard to IFN‐γ content. The pattern of differences between SF ‐and PB‐derived T cells was the same for the two groups of patients, but the SF CD4+ cells from RA patients produced significantly less IL‐2 than the corresponding cells from the non‐RA group. The difference between SF and PB T cells with regard to lymphokine production is probably related to various degrees of in vivo pre‐activation. The results do not indicate a major T‐cell deficiency in relation to lymphokine production in RA.
Gene transfer of master autophagy regulator TFEB results in clearance of toxic protein and correction of hepatic disease in alpha-1-anti-trypsin deficiency

Alpha‐1‐anti‐trypsin deficiency is the most common genetic cause of liver disease in children and liver transplantation is currently the only available treatment. Enhancement of liver autophagy increases degradation of mutant, hepatotoxic alpha‐1‐anti‐trypsin (ATZ). We investigated the therapeutic potential of liver‐directed gene transfer of transcription factor EB (TFEB), a master gene that regulates lysosomal function and autophagy, in PiZ transgenic mice, recapitulating the human hepatic disease. Hepatocyte TFEB gene transfer resulted in dramatic reduction of hepatic ATZ, liver apoptosis and fibrosis, which are key features of alpha‐1‐anti‐trypsin deficiency. Correction of the liver phenotype resulted from increased ATZ polymer degradation mediated by enhancement of autophagy flux and reduced ATZ monomer by decreased hepatic NFκB activation and IL‐6 that drives ATZ gene expression. In conclusion, TFEB gene transfer is a novel strategy for treatment of liver disease of alpha‐1‐anti‐trypsin deficiency. This study may pave the way towards applications of TFEB gene transfer for treatment of a wide spectrum of human disorders due to intracellular accumulation of toxic proteins.
Improving Early Childhood Educators' Understanding of the Value of Indoor Play through a Teacher Training Program.

A practicum project was designed to retrain preschool personnel on the importance of indoor play and on their role during play sessions. Workshops and training sessions were developed to examine the physical environment of the classroom, discuss play theories, and review skills that children can develop during play sessions. Preand posttests, along with classroom observations of teachers and children, revealed that most teachers who participated in the intervention changed their views and practices regarding indoor play. Teachers became both more aware of the role of play in helping children develop physical, emotional, cognitive, and social skills, and more involved in the children's play to promote these skills. Rooms were rearranged to allow for freedom of movement in and around newly created theme centers. The children became more focused on their role-playing, more verbal with their peers, and more involved in imaginative situations. (Six appendixes provide samples of preand posttest surveys and classroom observItion forms.) (MDM) *********************************************************************** Reproductions supplied by EDRS are the best that can be made from the original document. *********************************************************************** U S DEPARTMENT OP EDUCATION Office of Educational Research enct trnpeovement EDUCATIONAL RESOURCES INFORMATION CENTER IERICI NiitsthiS 6oCument has been reproduce0 as received from the person or organizabon frofg.libngit 0 Minot changes hcire been made to improve teoroducoon ouaItty Po.nIs of nrew or opinions slated in thiS document do not necessarily represent official OE RI position Of pOliCy Improving Early Childhood Educators' Understanding of the Value of Indoor Play Through A Teacher Training Program
Chromosomal G + C Content Evolution in Yeasts: Systematic Interspecies Differences, and GC-Poor Troughs at Centromeres

The G + C content at synonymous codon positions (GC3s) in genes varies along chromosomes in most eukaryotes. In Saccharomyces cerevisiae, regions of high GC3s are correlated with recombination hot spots, probably due to biased gene conversion. Here we examined how GC3s differs among groups of related yeast species in the Saccharomyces and Candida clades. The chromosomal locations of GC3s peaks and troughs are conserved among four Saccharomyces species, but we find that there have been highly consistent small shifts in their GC3s values. For instance, 84% of all S. cerevisiae genes have a lower GC3s value than their S. bayanus orthologs. There are extensive interspecies differences in the Candida clade both in the median value of GC3s (ranging from 22% to 49%) and in the variance of GC3s among genes. In three species—Candida lusitaniae, Pichia stipitis, and Yarrowia lipolytica—there is one region on each chromosome in which GC3s is markedly reduced. We propose that these GC-poor troughs indicate the positions of centromeres because in Y. lipolytica they coincide with the five experimentally identified centromeres. In P. stipitis, the troughs contain clusters of the retrotransposon Tps5. Likewise, in Debaryomyces hansenii, there is one cluster of the retrotransposon Tdh5 per chromosome, and all these clusters are located in GC-poor troughs. Locally reduced G + C content around centromeres is consistent with a model in which G + C content correlates with recombination rate, and recombination is suppressed around centromeres, although the troughs are unexpectedly wide (100–300 kb).
Mutagens in urine sampled repetitively from municipal refuse incinerator workers and water treatment workers.

Municipal refuse incinerator workers may be exposed to mutagenic compounds from combustion gases and particulates during plant operation, maintenance, and ash removal procedures. The frequency of mutagens was measured by the Ames assay in 3 urine samples collected from each of 37 workers in 4 refuse incinerators and 35 (control) workers from 8 water treatment plants during June-August 1990. When comparing the first urine samples contributed by workers in each cohort, incinerator workers had a significantly (p < .05) increased risk of both direct-acting mutagens and promutagens (8/37 or 22% for each mutagen type) compared with water treatment workers (2/35 or 6% for each mutagen type). Smoking within 24 h before urine sampling was not a confounder of these results. Interestingly, there was no significant (p > .05) difference for risk of urinary mutagens or promutagens between the two cohorts when comparing, respectively, the second and third urine samples from each cohort. The repeatability of demonstrating urinary mutagens in individual incinerator workers was poor, suggesting that their exposure was highly variable and/or that these workers modified their exposure (e.g., wore masks) as a consequence of being studied. Factors that influence production of mutagenic compounds during refuse incineration and subsequent worker exposure are discussed.
Novel Clinical Evidence of an Association between Homocysteine and Insulin Resistance in Patients with Hypothyroidism or Subclinical Hypothyroidism

Objective Hypothyroidism (HO) can induce significant metabolic dysfunction and increase cardiovascular disease risk. In the present study, we investigated the relationship between homocysteine (Hcy) and insulin resistance (IR) in patients with HO or subclinical hypothyroidism (SHO). Methods A total of 270 subjects were enrolled. All subjects were divided into the following three groups: HO, SHO and control. Plasma levels of Hcy were measured, and each patient’s homeostatic index of insulin resistance (HOMA-IR) was calculated. Statistical analyses were carried out to evaluate the correlations among groups and to determine the predictors of IR in patients with HO or SHO. Results The HOMA-IR value was significantly higher in the HO group than in the SHO and control groups. Plasma levels of Hcy were markedly increased in the HO group compared with those of the SHO group and controls. In addition, plasma levels of Hcy were positively correlated with the HOMA-IR values in both the HO and SHO groups. Multiple linear regression models showed that plasma levels of Hcy and free thyroxine (FT4) were the only predictors of HOMA-IR in patients with HO or SHO. Conclusions Plasma levels of Hcy and HOMA-IR were increased in patients with HO or SHO. Our results suggest that HO and SHO may increase the risk for atherogenesis and cardiovascular disease by increased IR. The increased IR induced by hyperhomocysteinemia in patients with HO or SHO may partially explain this adverse effect.
Customer-Oriented Selling: A Review, Extension, and Directions for Future Research

As customer-relationship selling continues to emerge as a significant selling strategy, the role of customer-oriented selling takes on added significance. The personal selling relationship literature lacks a comprehensive review that summarizes and organizes the empirical research regarding customer-oriented selling. Subsequently, it is unclear what exactly has been learned by research on customer-oriented selling and what questions remain to be answered. Therefore, this paper addresses these concerns by reviewing the current literature in customer-oriented selling, offering propositions to extend our understanding, providing directions for developing a customer-oriented sales force based on our current understanding, and offering suggestions for future research.
Bioassay and Characterization of Several Palmer Amaranth (Amaranthus palmeri) Biotypes with Varying Tolerances to Glyphosate

The wide distribution of Palmer amaranth (Amaranthus palmeri) in the southern US became a serious weed control problem prior to the extensive use of glyphosate-resistant crops. Currently glyphosate-resistant populations of Palmer amaranth occur in many areas of this geographic region creating an even more serious threat to crop production. Investigations were undertaken using four biotypes (one glyphosate-sensitive, one resistant from Georgia and two of unknown tolerance from Mississippi) of Palmer amaranth to assess bioassay techniques for the rapid detection and level of resistance in populations of this weed. These plants were characterized with respect to chlorophyll, betalain, and protein levels and immunological responses to an antibody of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) the target site of glyphosate. Only slight differences were found in four biotypes grown under greenhouse conditions regarding extractable soluble protein and chlorophyll content, but one biotype was found to be devoid of the red pigment, betalain. Measurement of early growth (seedling shoot elongation) of seedlings was a useful detection tool to determine glyphosate resistance. A leaf disc bioassay (using visual ratings and/or chlorophyll analysis) and an assay for shikimate accumulation were effective methods for determining herbicide resistance levels. The two unknown biotypes were found to be resistant to this herbicide. Some differences were found in the protein profiles of the biotypes, and western blots demonstrated a weak labeling of antibody in the glyphosate-sensitive biotype, whereas strong labeling occurred in the resistant plants. This latter point supports research by others, that increased copy number of the EPSPS gene (and increased EPSPS protein levels) is the resistance mechanism in this species. Results indicate the utility of certain bioassays for the determination of resistance and provide useful comparative information on the levels of inherent constituents among closely related plants.
The Case for Permanent Foster Care

This article reviews historical developments in the social institution of foster care, leading up to the permanent planning revolution and current family preservation policies in the United States. Success rates of adoption, family preservation and family reunification programs are examined and a rationale for the inclusion of permanent foster care as an option for children is presented. Permanent foster care has several advantages: (1) it is federally and automatically funded; (2) it can lead to increased supervision of foster parents; (3) it creates more permanence for more children; (4) it promotes attachment through ensuring both child and foster parent stability. Models for permanent foster care already exist, in long-term foster care arrangements for special-needs children and in subsidized adoption
International Journal of C 2004 Institute for Scientific Numerical Analysis and Modeling Computing and Information Polynomial Preserving Gradient Recovery and a Posteriori Estimate for Bilinear Element on Irregular Quadrilaterals

A polynomial preserving gradient recovery method is proposed and analyzed for bilinear element under quadrilateral meshes. It has been proven that the recovered gradient converges at a rate O(h 1+ρ) for ρ = min(α, 1), when the mesh is distorted O(h 1+α) (α > 0) from a regular one. Consequently, the a posteriori error estimator based on the recovered gradient is asymptotically exact.
Mesolimbic Functional Magnetic Resonance Imaging Activations during Reward Anticipation Correlate with Reward-Related Ventral Striatal Dopamine Release

The dopaminergic mechanisms that control reward-motivated behavior are the subject of intense study, but it is yet unclear how, in humans, neural activity in mesolimbic reward-circuitry and its functional neuroimaging correlates are related to dopamine release. To address this question, we obtained functional magnetic resonance imaging (fMRI) measures of reward-related neural activity and [11C]raclopride positron emission tomography measures of dopamine release in the same human participants, while they performed a delayed monetary incentive task. Across the cohort, a positive correlation emerged between neural activity of the substantia nigra/ventral tegmental area (SN/VTA), the main origin of dopaminergic neurotransmission, during reward anticipation and reward-related [11C]raclopride displacement as an index of dopamine release in the ventral striatum, major target of SN/VTA dopamine neurons. Neural activity in the ventral striatum/nucleus accumbens itself also correlated with ventral striatal dopamine release. Additionally, high-reward-related dopamine release was associated with increased activation of limbic structures, such as the amygdala and the hippocampus. The observed correlations of reward-related mesolimbic fMRI activation and dopamine release provide evidence that dopaminergic neurotransmission plays a quantitative role in human mesolimbic reward processing. Moreover, the combined neurochemical and hemodynamic imaging approach used here opens up new perspectives for the investigation of molecular mechanisms underlying human cognition.
Multilocus Genotyping of Human Giardia Isolates Suggests Limited Zoonotic Transmission and Association between Assemblage B and Flatulence in Children

Background Giardia intestinalis is one of the most common diarrhea-related parasites in humans, where infection ranges from asymptomatic to acute or chronic disease. G. intestinalis consists of eight genetically distinct genotypes or assemblages, designated A–H, and assemblages A and B can infect humans. Giardiasis has been classified as a possible zoonotic disease but the role of animals in human disease transmission still needs to be proven. We tried to link different assemblages and sub-assemblages of G. intestinalis isolates from Swedish human patients to clinical symptoms and zoonotic transmission. Methodology/Principal Findings Multilocus sequence-based genotyping of 207 human Giardia isolates using three gene loci: ß-giardin, glutamate dehydrogenase (gdh), and triose phosphate isomerase (tpi) was combined with assemblage-specific tpi PCRs. This analysis identified 73 patients infected with assemblage A, 128 with assemblage B, and six with mixed assemblages A+B. Multilocus genotypes (MLGs) were easily determined for the assemblage A isolates, and most patients with this genotype had apparently been infected through anthroponotic transmission. However, we also found evidence of limited zoonotic transmission of Giardia in Sweden, since a few domestic human infections involved the same assemblage A MLGs previously reported in Swedish cats and ruminants. Assemblage B was detected more frequently than assemblage A and it was also more common in patients with suspected treatment failure. However, a large genetic variability made determination of assemblage B MLGs problematic. Correlation between symptoms and assemblages was found only for flatulence, which was significantly more common in children less than six years of age infected with assemblage B. Conclusions/Significance This study shows that certain assemblage A subtypes are potentially zoonotic and that flatulence is connected to assemblage B infections in young children. Determination of MLGs from assemblages A and B can be a valuable tool in outbreak situations and to help identify possible zoonotic transmission.
FREE AMINO ACIDS OF FETAL BRAIN

Abstract— Injection of each of the branched chain amino acids into maternal rats from the 14th to 21st day of pregnancy resulted in increased levels of these compounds in fetal serum. Furthermore, these amino acids were found in high concentration in fetal brain. The elevated levels of the branched chain amino acids were associated with a disturbance of the free amino acid pool in the brain. Isoleucine appeared to have the greatest effect in depressing the other amino acids.
Software-based self-testing of embedded processors

Embedded processor testing techniques based on the execution of self-test programs have been recently proposed as an effective alternative to classic external tester-based testing and pure hardware built-in self-test (BIST) approaches. Software-based self-testing is a nonintrusive testing approach and provides at-speed testing capability without any hardware or-performance overheads. In this paper, we first present a high-level, functional component-oriented, software-based self-testing methodology for embedded processors. The proposed methodology aims at high structural fault coverage with low test development and test application cost. Then, we validate the effectiveness of the proposed methodology as a low-cost alternative over structural software-based self-testing methodologies based on automatic test pattern generation and pseudorandom testing. Finally, we demonstrate the effectiveness and efficiency of the proposed methodology by completely applying it on two different processor implementations of a popular RISC instruction set architecture including several gate-level implementations.
Humility attenuates negative attitudes and behaviors toward religious out-group members

Humility is marked by the regulation of selfish impulses for the sake of others, including holding a modest view of one’s beliefs (and their relative strengths and weaknesses). In three studies, we evaluated the extent to which humility attenuates negative attitudes, behavioral intentions, and behaviors toward religious out-group members. In Study 1 (N = 159), humility regarding religious beliefs was associated with positive attitudes toward religiously different individuals. In Study 2 (N = 149), relational and intellectual humility were associated with less aggressive behavioral intentions in a hypothetical situation in which their cherished beliefs were criticized. In Study 3 (N = 62), participants implicitly primed with humility administered significantly less hot sauce (a behavioral measure of aggression) to a religious out-group member who criticized their cherished views relative to participants in the neutral prime condition. We highlight the importance of humility in promoting positive attitudes and behaviors toward religious out-group members.
Universities and the future of America

Since World War II, says the author, industrialized nations have come to depend so heavily on expert knowledge, scientific discovery, and highly trained personnel that universities have become "the central institution in postindustrial society. " "If universities are so important to society and if ours are so superior, one might have thought that America would be flourishing in comparison to other industrialized countries of the world. Yet this is plainly not the case.Our economic position in the world has deteriorated [and] we have climbed to the top, or near the top, of all advanced countries in the percentage of population who live in poverty, commit crimes, become addicted to drugs, have illegitimate children, or are classified as functionally illiterate. " In light of these results, "it is fair to ask whether our universities are doing all they can and should to help America surmount the obstacles that sap our economic strength and blight the lives of millions of our people. " Having posed this question, Derek Bok reviews what science can do to bring about greater productivity, what professional schools can do to improve the effectiveness of corporations, government, and public education, and what all parts of the university are doing to help students acquire higher levels of ethical and social responsibility. He concludes that Universities are contributing much less than the should to help the nation address its most urgent social problems. "A century after the death of Cardinal Newman, many university officials and faculty members continue to feel ambivalent about deliberate efforts to address practical problems of society. And though competition drives university leaders and their faculties to unremitting effort, what competition rewards is chiefly success in fields that command academic prestige rather than success in responding to important social needs. " Bok urges academic leaders, trustees, foundations, and government agencies to work together to help universities realign their priorities "so that they will be ready to make their full contribution when the nation turns its attention again to the broad agenda of reform.Observing our difficulties competing abroad, our millions of people in poverty, our drug-ridden communities, our disintegrating families, our ineffective schools, those who help to shape our universities have reason to ask whether they too have any time to lose. "