Miracle-dz/UniFAR-FTUs huggingface dataset:
High-speed detection of occult tumor cells in peripheral blood

Although detection of tumor cells in peripheral blood using imitiunocytochemistry and optical scanning is a promising method for screening and monitoring cancer, it poses a major technical challenge due to the extremely low tumor cell concentration in blood. The preferred detection method - digital microscopy - is far too slow for analysis of the large numbers of cells required for statistical validity. We describe here a novel prescan instrument that rapidly identifies a small number of candidates for subsequent examination by digital microscopy to determine if they are genuine tumor cells. The prescan is 500 times faster than digital microscopy and yet has a similar sensitivity. The high prescan speed is accomplished by trading resolution for field of view. The resolution of the prescan is determined by the laser spot size of about 10 microns. While this resolution is much coarser than the submicron resolution of microscopes, it is still sufficient for detecting fluorescent cells because it matches the size of a typical cell. The wide field of view and high scan rate are enabled by a novel application of fiber optics.
Roles of Forkhead-box Transcription Factors in Controlling Development, Pathogenicity, and Stress Response in Magnaporthe oryzae

Although multiple transcription factors (TFs) have been characterized via mutagenesis to understand their roles in controlling pathogenicity and infection-related development in Magnaporthe oryzae, the causal agent of rice blast, if and how forkhead-box (FOX) TFs contribute to these processes remain to be characterized. Four putative FOX TF genes were identified in the genome of M. oryzae, and phylogenetic analysis suggested that two of them (MoFKH1 and MoHCM1) correspond to Ascomycota-specific members of the FOX TF family while the others (MoFOX1 and MoFOX2) are Pezizomycotina-specific members. Deletion of MoFKH1 (ΔMofkh1) resulted in reduced mycelial growth and conidial germination, abnormal septation and stress response, and reduced virulence. Similarly, ΔMohcm1 exhibited reduced mycelial growth and conidial germination. Conidia of ΔMofkh1 and ΔMohcm1 were more sensitive to one or both of the cell cycle inhibitors hydroxyurea and benomyl, suggesting their role in cell cycle control. On the other hand, loss of MoFOX1 (ΔMofox1) did not show any noticeable changes in development, pathogenicity, and stress response. Deletion of MoFOX2 was not successful even after repeated attempts. Taken together, these results suggested that MoFKH1 and Mo-HCM1 are important in fungal development and that MoFKH1 is further implicated in pathogenicity and stress response in M. oryzae.
Flexural Behaviour of Carbon Textile-Reinforced Concrete with Prestress and Steel Fibres

Four-point bending tests were adopted to investigate the influences of the number of textile layers, volume content of steel fibres, and prestress on the flexural behaviour of carbon textile-reinforced concrete (TRC). The failure mode of the specimen changed from debonding failure to shear failure, accompanied by the matrix-textile interfacial debonding with an increasing number of textile layers. The interfacial bonding performance between the textile and matrix improved with the addition of steel fibres in the TRC specimens. The presence of prestress or steel fibres improved first-crack and ultimate stresses of the TRC specimen. In comparison with the first-crack stress, a more pronounced enhancement in the ultimate stress was achieved by the addition of steel fibres. However, the effect of prestress on the first-crack stress was found to be more significant than on the ultimate stress. The prestress combined with steel fibres further improved the flexural behaviour of the TRC specimens. The prestressed TRC specimens with 1% volume content of steel fibres effectively avoided debonding. Thus, the utilization of the textiles could be improved.
Growth of the Escherichia coli cell surface

Phage T6 was used as a label to follow the growth of the outer membrane in a strain of Escherichia coli temperature sensitive for the production of the T6 receptor. Extension of the surface takes place at the cell poles. Small cells extend at only one pole, whereas larger cells grow from both poles. The change from unipolar to bipolar growth appears to depend on the attainment of a particular cell size and not on completion of chromosome replication.
Gene Transfection of H25A Mutant Heme Oxygenase-1 Protects Cells against Hydroperoxide-induced Cytotoxicity*

Heme oxygenase (HO)-1 is a stress-inducible enzyme protecting cells against oxidative stress, and mechanisms have been considered to depend exclusively on its enzyme activity. This study aimed to examine if the protein lacking catalytic activities could also display such resistance against oxidative stress. Stable transfectants of rat wild type HO-1 cDNA (HO-1-U937) and those of its H25A mutant gene (mHO-1-U937) were established using human monoblastic lymphoma cell U937. HO-1-U937 and mHO-1-U937 used in the study exhibited similar levels of the protein expression, while only the former increased enzyme activities. HO-1- and mHO-1 U937 cells became more and less sensitive to H2O2than mock transfectants, respectively; such distinct susceptibility between the cells was ascribable to differences in the capacity to scavenge H2O2 through catalase and to execute iron-mediated oxidant propagation. On the other hand, both cell lines exhibited greater resistance to tert-butyl hydroperoxide than mock transfectants. The resistance of HO-1-U937 to hydroperoxides appeared to result from antioxidant properties of bilirubin, an HO-derived product, while that of mHO-1-U937 was ascribable to increased contents of catalase and glutathione. These results provided evidence that gene transfection of the activity-lacking mutant HO-1 protects cells against oxidative stress through multiple mechanisms involving up-regulation of catalase and glutathione contents.
Seasonality, incidence and prognosis in atrial fibrillation and stroke in Denmark and New Zealand

Objectives There are relatively few large studies of seasonal variation in the occurrence of stroke in patients with atrial fibrillation (AF). We investigated the seasonal variation in incidence rates of hospitalisation with stroke in patients from Denmark and New Zealand. Design Cohort study. Setting Nationwide hospital discharge data from Denmark and New Zealand. Participants 243 381 (median age 75) subjects having a first-time hospitalisation with AF in Denmark and 51 480 (median age 76) subjects in New Zealand constituted the study population. Subjects with previous hospitalisation with stroke were excluded. Primary and secondary effect measures Peak-to-trough ratio of the seasonal variation in incidence rates of stroke in AF patients adjusted for an overall trend was primary effect measure and was assessed using a log-linear Poisson regression model. Secondary effect measures were incidence rate ratios of AF and 30-day case fatality for stroke patients. Results Incidence rates of AF per 1000 person-years in Denmark increased by 5.4% (95% CI 5.3% to 5.7%) for patients aged <65 and 5% (95% CI 4.9% to 5.1%) for patients aged ≥65, whereas the increase was 0.2% (95% CI −0.2% to 0.6%) for patients aged <65 and 2.6% (95% CI 2.4% to 2.8%) for patients aged ≥65 in New Zealand. In Denmark 36 088 subjects were hospitalised with stroke, and 7518 subjects in New Zealand, both showing peaks during winter with peak-to-trough ratios of 1.22 and 1.27, respectively and a decreasing trend. The 30-day case fatality risk for stroke patients having AF is now (2000–2008) about 20% in both countries. Conclusions Although incidence rates of hospitalisation with stroke in patients with AF have decreased in recent years, stroke remains a common AF complication with a high case fatality risk. The marked winter peak in incidence rates of hospitalisation with stroke in AF patients suggests that there are opportunities to reduce this complication. Further studies are necessary to identify how to optimise treatment of AF and prevention of stroke.
Distress levels in pregnant and matched non‐pregnant women

This study examined self‐rated symptoms of distress (Depression Anxiety and Stress Scale—DASS‐21 and Perceived Stress Scale—PSS‐4) among 93 pregnant women and a comparison group of 93 non‐pregnant women matched on age and educational attainment. There were no significant differences between the groups, either on mean levels of distress or on proportions above a clinical cut‐off point. Overall, 22%, 31% and 16% of pregnant women reported experiencing at least moderate levels of depression, anxiety and stress, respectively. Implications for conceptualising distress in pregnancy and identifying and providing support for the substantial minority who are distressed are discussed.
The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo

Cytoplasmic Polyadenylation Element Binding protein (CPEB) is an RNA binding protein involved in dendritic delivery of mRNA and activity-dependent, polyadenylation-induced translation of mRNAs in the dendritic arbor. CPEB affects learning and memory and impacts neuronal morphological and synaptic plasticity. In neurons, CPEB is concentrated in ribonucleoprotein (RNP) granules that distribute throughout the dendritic arbor and localize near synapses, suggesting that the trafficking of RNP granules is important for CPEB function. We tagged full-length CPEB and an inactive mutant CPEB with fluorescent proteins, then imaged rapid dendritic branch dynamics and RNP distribution using two-photon time-lapse microscopy of neurons in the optic tectum of living Xenopus laevis tadpoles. Though the inactive CPEB mutant transports mRNA in the dendritic arbor, its expression interferes with CPEB-dependent translation because it is incapable of activity-triggered mRNA polyadenylation. In dendrites, the distributions of the active and inactive CPEB-containing RNP granules do not differ; the RNP granules are dense and their positions do not correlate with sites of rapid dendritic branch dynamics or the eventual fate of the dendritic branches. Because CPEB's sensitivity to activity-dependent signaling does not alter its dendritic distribution, it indicates that active sites in the dendritic arbor are not targeted for RNP granule localization. Nevertheless, inactive CPEB accumulates in granules in terminal dendritic branches, supporting the hypothesis that upon activation CPEB and its mRNA cargo are released from granules and are then available for dendritic translation.
Sensitivity Analysis of Limit-Cycle Oscillating Hybrid Systems

A theory is developed for local, first-order sensitivity analysis of limit-cycle oscillating hybrid systems, which are dynamical systems exhibiting both continuous-state and discrete-state dynamics whose state trajectories are closed, isolated, and time-periodic. Methods for the computation of initial-condition sensitivities and parametric sensitivities are developed to account exactly for any jumps in the sensitivities at discrete transitions and to exploit the time-periodicity of the system. It is shown that the initial-condition sensitivities of any limit-cycle oscillating hybrid system can be represented as the sum of a time-decaying component and a time-periodic component so that they become periodic in the long-time limit. A method is developed for decomposition of the parametric sensitivities into three parts, characterizing the influence of parameter changes on period, state variable amplitudes, and relative phases, respectively. The computation of parametric sensitivities of period, amplitudes, and different types of phases is also described. The methods developed in this work are applied to particular models for illustration, including models exhibiting state variable jumps.
Time, Chance and Reduction: Irreversibility in stochastic dynamics

Over recent decades, some approaches to non-equilibrium statistical mechanics, that differ decidedly in their foundational and philosophical outlook, have nevertheless converged in developing a common unified mathematical framework. I will call this framework ‘stochastic dynamics’, since the main characteristic feature of the approach is that it characterizes the evolution of the state of a mechanical system as evolving under stochastic maps, rather than under a deterministic and time-reversal invariant Hamiltonian dynamics.1 The motivations for adopting this stochastic type of dynamics come from at least three different viewpoints. 1. “Coarse graining”(cf. van Kampen 1962, Penrose 1970): In this view one assumes that on the microscopic level the system can be characterized as a (Hamiltonian) dynamical system with deterministic time-reversal invariant dynamics. However, on the macroscopic level, one is only interested in the evolution of macroscopic states, i.e. in a partition (or coarse graining) of the microscopic phase space into discrete cells. The usual idea is that the form and size of these cells are chosen in accordance with the limits of our observational capabilities. On the macroscopic level, the evolution now need no longer be portrayed as deterministic. When only the macrostate of a system at an instant is given, it is in general not fixed what its later macrostate will be, even if the underlying microscopic evolution is deterministic. Instead, one can provide transition probabilities, that specify how probable the transition from any given initial macrostate to later macrostates is. Although it is impossible, without further assumptions, to say anything general about the evolution of the macroscopically characterized states, it is possible to describe the evolution of an ensemble or a probability distribution over these states, in terms of a stochastic process. 2. “Interventionism”, or “open systems” (cf. Blatt 1959, Davies 1976, Lindblad 1976, Lindblad 1983, Ridderbos 2002): On this view, one assumes that the system to be described is not isolated but in (weak) interaction with the environment. It is assumed that the total system, consisting of the system of interest and the environment can be described as a (Hamiltonian) dynamical system with a time-reversal invariant and deterministic dynamics. If we represent the state of the system by x ∈ Γ and that of the environment by y ∈ Γ, their joint evolution is
ECAP, ESR and Subjective Levels for Two Different Nucleus 24 Electrode Arrays

Objective: We compared behavioral judgment of maximum comfortable loudness levels (C levels) and behavioral thresholds (Ts) for straight and Contour electrode arrays with two objective thresholds, electrically elicited stapedial reflex thresholds (eSRTs) and electrically elicited compound action potential thresholds (eCAP thresholds), on experienced adult cochlear implant users. Next, we evaluated the predictive value of objective measures for the straight and Contour electrode arrays, respectively. Study Design: This is a prospective, two-group comparison study of two objective and subjective levels for two different Nucleus 24 electrode arrays. Patients: Thirty experienced adults with Nucleus 24 cochlear implant were subjects in this study. Half the subjects used the straight electrode array, and the other half used the Contour electrode array. Methods: Subjective C levels, Ts, and eSRTs were successfully identified for each active electrode. eCAP thresholds were measured on 5 representative basal, medial, and apical electrodes. Correlation and regression analyses between subjective levels and objective thresholds were performed. Results: For our study subjects, there were no significant differences between the straight and Contour electrode array in regard to stimulation requirements between C levels, Ts, and thresholds of eCAP thresholds and eSRTs. Conclusion: Both eSRTs and eCAP thresholds may be used equally for estimation of subjective levels for either straight electrode array or Contour electrode array.
Attitudes Toward Psychiatry as a Prospective Career among Medical Students in Their Pre-Clinical Year in China- A Pilot Study

Objective To understand the attitudes among medical students in China toward different medical specialties and to find the factors that influenced their choice of career in psychiatry. Methods A questionnaire was developed and administered to 287 medical students at the Xiangya Medical College, Central South University in Changsha, China. All the students were asked to rate the importance of five possible factors in choosing a specialty as a vocation: the ability to help patients, interesting and challenging work, lifestyle factors, financial reward, and prestige. Results Students reported negative perceptions of psychiatry in regard to all five possible factors that were important in choosing a specialty as a vocation, especially in financial reward and prestige. Conclusions Medical students in China have negative attitudes toward psychiatry as a career. Some negative beliefs about psychiatry seem to be due to erroneous or insufficient knowledge that could be corrected during the course of medical education. Some negative attitudes were unlikely to be completely changed until the mental health system in China improves.
Identification of White Spot Syndrome Virus Latency-Related Genes in Specific-Pathogen-Free Shrimps by Use of a Microarray

ABSTRACT To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
Specific reduction in cortisol stress reactivity after social but not attention-based mental training

Social but not attention-based mental training reduces cortisol reactivity to psychosocial stress. Psychosocial stress is a public health burden in modern societies. Chronic stress–induced disease processes are, in large part, mediated via the activation of the hypothalamic-pituitary-adrenal (HPA) axis and the sympathetic-adrenal-medullary system. We asked whether the contemplative mental training of different practice types targeting attentional, socio-affective (for example, compassion), or socio-cognitive abilities (for example, perspective-taking) in the context of a 9-month longitudinal training study offers an effective means for psychosocial stress reduction. Using a multimethod approach including subjective, endocrine, autonomic, and immune markers and testing 313 participants in a standardized psychosocial laboratory stressor, we show that all three practice types markedly reduced self-reported stress reactivity in healthy participants. However, only the training of intersubjective skills via socio-affective and socio-cognitive routes attenuated the physiological stress response, specifically the secretion of the HPA axis end-product cortisol, by up to 51%. The assessed autonomic and innate immune markers were not influenced by any practice type. Mental training focused on present-moment attention and interoceptive awareness as implemented in many mindfulness-based intervention programs was thus limited to stress reduction on the level of self-report. However, its effectiveness was equal to that of intersubjective practice types in boosting the association between subjective and endocrine stress markers. Our results reveal a broadly accessible low-cost approach to acquiring psychosocial stress resilience. Short daily intersubjective practice may be a promising method for minimizing the incidence of chronic social stress–related disease, thereby reducing individual suffering and relieving a substantial financial burden on society.
Video Inpainting of Complex Scenes

We propose an automatic video inpainting algorithm which relies on the optimisation of a global, patch-based functional. Our algorithm is able to deal with a variety of challenging situations which naturally arise in video inpainting, such as the correct reconstruction of dynamic textures, multiple moving objects and moving background. Furthermore, we achieve this in an order of magnitude less execution time with respect to the state-of-the-art. We are also able to achieve good quality results on high definition videos. Finally, we provide specific algorithmic details to make implementation of our algorithm as easy as possible. The resulting algorithm requires no segmentation or manual input other than the definition of the inpainting mask, and can deal with a wider variety of situations than is handled by previous work. 1. Introduction. Advanced image and video editing techniques are increasingly common in the image processing and computer vision world, and are also starting to be used in media entertainment. One common and difficult task closely linked to the world of video editing is image and video " inpainting ". Generally speaking, this is the task of replacing the content of an image or video with some other content which is visually pleasing. This subject has been extensively studied in the case of images, to such an extent that commercial image inpainting products destined for the general public are available, such as Photoshop's " Content Aware fill " [1]. However, while some impressive results have been obtained in the case of videos, the subject has been studied far less extensively than image inpainting. This relative lack of research can largely be attributed to high time complexity due to the added temporal dimension. Indeed, it has only very recently become possible to produce good quality inpainting results on high definition videos, and this only in a semi-automatic manner. Nevertheless, high-quality video inpainting has many important and useful applications such as film restoration, professional post-production in cinema and video editing for personal use. For this reason, we believe that an automatic, generic video inpainting algorithm would be extremely useful for both academic and professional communities.
"spatial-Temporal" Patterns in Prokaryote genomes

We have developed a simple scheme to visualize the string composition of long DNA sequences and applied it to all available prokaryote complete genomes. Each species has a specific "portrait" and most clearly seen patterns in these portraits are determined by short avoided and under-represented strings. The "spatial" patterns in the space of short strings might reflect some "temporal" events in the evolutionary history.