Miracle-dz/UniFAR-FTUs huggingface dataset:
Egg Consumption and Coronary Heart Disease: An Epidemiologic Overview

Serum cholesterol has been established as a modifiable risk factor for coronary heart disease. Experimental feeding studies show that saturated fat and cholesterol increase serum cholesterol levels; thus, dietary recommendations for lowering the risk of heart disease proscribe the intake of both substances. Recommendations have also included limits on the intake of eggs because of their high cholesterol content. In free-living populations, diet reflects a pattern of associated choices. Increases in one food may lead to changes in the consumption of other foods that may modulate disease risk. Epidemiologic data are helpful in assessing the importance of foods and nutrients in the context in which they are actually consumed. We review epidemiologic data relating dietary cholesterol and eggs to coronary disease risk. Cholesterol intake was associated with a modest increase in the risk of coronary events. The true magnitude of the association is difficult to estimate because most studies fail to account for potential confounding by other features of the diet. When a full-range of confounding factors was considered, the association between cholesterol intake and heart disease risk was small (6% increase in risk for 200mg/1,000kcal/day difference in cholesterol intake). Several studies have examined egg intake and its relationship with coronary outcomes. All but one failed to consider the role of other potentially confounding dietary factors. When dietary confounders were considered, no association was seen between egg consumption at levels up to 1+ egg per day and the risk of coronary heart disease in non-diabetic men and women.
CD34 is required for dendritic cell trafficking and pathology in murine hypersensitivity pneumonitis.

RATIONALE Although recent work has shown that CD34 plays an important role in the trafficking of inflammatory cells during Th2-biased inflammatory responses, its role in Th1/Th17-biased disease as well as dendritic cell (DC) trafficking is unknown. OBJECTIVES We used CD34-deficient mice (Cd34(-/-)) to investigate the role of CD34 in the Th1/Th17-biased lung inflammatory disease, hypersensitivity pneumonitis (HP). METHODS HP was induced in wild-type (wt) and Cd34(-/-) mice by repeated intranasal administration of Saccharopolyspora rectivirgula antigen. Lung inflammation was assessed by histology and analysis of bronchoalveolar lavage cells. Primary and secondary immune responses were evaluated by cytokine recall responses of pulmonary inflammatory cells as well as draining lymph node cells. MEASUREMENTS AND MAIN RESULTS Cd34(-/-) mice were highly resistant to the development of HP and exhibited an inflammatory pattern more reflective of a primary response to S. rectivirgula rather than the chronic lymphocytosis that is typical of this disease. Cytokine recall responses from Cd34(-/-) lymph node cells were dampened and consistent with a failure of antigen-loaded Cd34(-/-) DCs to deliver antigen and prime T cells in the draining lymph nodes. In agreement with this interpretation, adoptive transfer of wt DCs into Cd34(-/-) mice was sufficient to restore normal sensitivity to HP. CD34 was found to be expressed by wt DCs, and Cd34(-/-) DCs exhibited an impaired ability to chemotax toward a subset of chemokines in vitro. Finally, expression of human CD34 in Cd34(-/-) mice restored normal susceptibility to HP. CONCLUSIONS We conclude that CD34 is expressed by mucosal DCs and plays an important role in their trafficking through the lung and to the lymph nodes. Our data also suggest that CD34 may play a selective role in the efficient migration of these cells to a subset of chemokines.
A systematic analysis of spring symmetry on optimality of antagonistic variable stiffness actuation

We present a systematic co-design optimization framework that enables comparison of antagonist variable stiffness actuators (VSA) with and without spring symmetry constraints. The proposed framework promotes a fair comparative analysis by maintaining design continuity among system-optimal designs of symmetric and asymmetric VSA configurations. Through a case study of a VSA-powered knee prosthesis, we not only demonstrate that co-design of robots driven by VSAs can provide important performance benefits with respect to sub-system level control-optimal designs, but also provide evidence that relaxing symmetry constraints for certain periodic tasks can result in substantial advantages in performance. Our systematic comparative analysis justifies the use of asymmetric stiffness arrangements for antagonistic VSA-powered robotic systems performing periodic tasks.
Strongly bound citrate stabilizes the apatite nanocrystals in bone

Nanocrystals of apatitic calcium phosphate impart the organic-inorganic nanocomposite in bone with favorable mechanical properties. So far, the factors preventing crystal growth beyond the favorable thickness of ca. 3 nm have not been identified. Here we show that the apatite surfaces are studded with strongly bound citrate molecules, whose signals have been identified unambiguously by multinuclear magnetic resonance (NMR) analysis. NMR reveals that bound citrate accounts for 5.5 wt% of the organic matter in bone and covers apatite at a density of about 1 molecule per (2 nm)2, with its three carboxylate groups at distances of 0.3 to 0.45 nm from the apatite surface. Bound citrate is highly conserved, being found in fish, avian, and mammalian bone, which indicates its critical role in interfering with crystal thickening and stabilizing the apatite nanocrystals in bone.
Pyrosequencing-Based Comparative Genome Analysis of Vibrio vulnificus Environmental Isolates

Between 1996 and 2006, the US Centers for Disease Control reported that the only category of food-borne infections increasing in frequency were those caused by members of the genus Vibrio. The gram-negative bacterium Vibrio vulnificus is a ubiquitous inhabitant of estuarine waters, and is the number one cause of seafood-related deaths in the US. Many V. vulnificus isolates have been studied, and it has been shown that two genetically distinct subtypes, distinguished by 16S rDNA and other gene polymorphisms, are associated predominantly with either environmental or clinical isolation. While local genetic differences between the subtypes have been probed, only the genomes of clinical isolates have so far been completely sequenced. In order to better understand V. vulnificus as an agent of disease and to identify the molecular components of its virulence mechanisms, we have completed whole genome shotgun sequencing of three diverse environmental genotypes using a pyrosequencing approach. V. vulnificus strain JY1305 was sequenced to a depth of 33×, and strains E64MW and JY1701 were sequenced to lesser depth, covering approximately 99.9% of each genome. We have performed a comparative analysis of these sequences against the previously published sequences of three V. vulnificus clinical isolates. We find that the genome of V. vulnificus is dynamic, with 1.27% of genes in the C-genotype genomes not found in the E- genotype genomes. We identified key genes that differentiate between the genomes of the clinical and environmental genotypes. 167 genes were found to be specifically associated with environmental genotypes and 278 genes with clinical genotypes. Genes specific to the clinical strains include components of sialic acid catabolism, mannitol fermentation, and a component of a Type IV secretory pathway VirB4, as well as several other genes with potential significance for human virulence. Genes specific to environmental strains included several that may have implications for the balance between self-preservation under stress and nutritional competence.
Study of the Influence of Ethanol on Basic Fibroblast Growth Factor Structure Influence of Ethanol on Basic Fibroblast Growth Factor Structure

The growth of cells is controlled by stimulatory or inhib-itory factors. More than twenty different families of polypeptide growth factors have been structurally and functionally characterized. Basic fi-broblast growth factor (bFGF) of the fibroblast growth factor family was characterized in 1974 as having proliferative activity for fibroblastic cells. The inhibitory effects of ethanol on cell proliferation result from interference with mitogenic growth factors (e.g., bFGF, EGF and PDGF). In order to better understand the mode of action of bFGF, particularly regarding the influence of ethanol on the biological activity of bFGF, three recombinant bFGF mutants were produced (M6B-bFGF, M1-bFGF and M1Q-bFGF). In the present study, wild bFGF and these mutants were examined by molecular dynamics simulations in systems consisting of a solute molecule in ethanol solution at 298 K and physiological pH over 4.0 ns. The hydrogen bonds, the root mean square deviations and specific radial distribution functions were employed to identify changes in the hydrogen bond structures, in the stability and in the approximation of groups in the different peptides to get some insight into the biological role of specific bFGF regions. The detailed description of the intramo-lecular hydrogen bonds, hydration, and intermolecular hydrogen bonds taking place in bFGF and its mutants in the presence of ethanol established that the residues belonging to the β5 and β9 strands, especially SER-73(β5), TYR-112(β9), THR-114(β9), TYR-115(β9), and SER-117(β9), are the regions most affected by the presence of ethanol molecules in solution.
Hierarchical Clustering of Dynamical Networks Using a Saddle-Point Analysis

This paper studies cluster synchronization in dynamical networks. A class of cooperative dynamical networks that exhibit clustering in their asymptotic behavior is analyzed. The network nodes are equipped with heterogeneous dynamics and interact with a nonlinear and saturated interaction rule. It is proven that cluster synchronization appears asymptotically independent of the initial conditions. The clustering behavior of the dynamic network is shown to correspond to the solution of a static saddle-point problem, enabling a precise characterization of the clustering structure. We show how the clustering structure depends on the relation between the underlying graph, the node dynamics, and the saturation level of the interactions. This interpretation leads to deeper combinatorial insights related to clustering, including a generalization of classical network partitioning problems such as the inhibiting bisection problem, the min s-t-cut problem, and hierarchical clustering analysis. The theoretical results are applied for the analysis of a test-case network, inspired by the IEEE 30-bus system.
Cancer Stem Cells (CSCs) in Lung Cancer

The cancer stem cell model for tumor progression is the first model of its type to suggest that only one subpopulation of cancer cells is capable of proliferating indefinitely. These cells resemble normal stem cells in their capacity for self-renewal and multi-potential differentiation, and can both initiate and maintain tumors. The prevailing names for these cells are “cancer stem cells (CSCs)” and “cancer initiating cells (CICs). ” The CSC model implies a hierarchical organization within the tumor in which a limited number of CSCs represents the apex of the hierarchy. CSCs are chemo-resistant, radio-resistant, and quiescent, and have been shown to cause both metastasis and relapse. CSCs were first described in patients with acute myeloid leukemia (AML) by Dick et al. (Lapidot et al., 1994). As to be expected from leukemia stem cells, these CSCs exhibited the properties of self-renewal, proliferation and multipotency. The frequency of these leukemiainitiating cells in the peripheral blood of those AML patients was one engraftment unit in 250,000 cells. Dick et al. identified the leukemia-initiating cells as CD34+CD38-. In the years since, CSCs have been identified in cancers of the breast(Al-Hajj et al., 2003), brain (Singh et al., 2003) and prostate, pancreas and lung (Eramo et al., 2008). In this chapter we review CSCs in lung cancer.
Innervation of the rabbit ductus deferens after vasectomy and vasovasostomy.

The urethralend of the ductus deferens in New Zealand white rabbitsbilaterallyvasecomized retained normal adrenergic innervation as evaluated by fibers identified with formalin-induced fluorescence. The testicular end of the ductus deferens and the caudal epididymal innervation were severely compromised. The site of the vasectomy may affect the degree of denervation in that inguinal vasectomies destroyed most, but not all, innervation, whereas vasectomies performed adjacent to the caudal epididymis (scrotal) caused total denervation. With vasovasostomy 30 days after vasectomy, significant reinnervation of the ductus deferens was observed by 28 days after the second operation. At the vasovasostomy site the nerve fibers were tortuously swirled and innervation was less than in the adjacent urethral and testicular sections of the ductus deferens. Such a zone may impede the flow of sperm during sexual excitement while not significantly affecting slow filling. Reinnervation of the caudal epididymis was slower and apparently less complete than that of the ductus deferens; by day 49 post vasovasostomy, only 20% of the normal innervationdensity was found.
The Impact of Family Intactness on Family Functioning, Parental Control, and Parent–Child Relational Qualities in a Chinese Context

The current study investigated the differences between intact and non-intact families in family processes, including systematic family functioning, parental behavioral control, parental psychological control, and parent–child relational qualities. The participants were 3,328 Secondary One students, with a mean age of 12.59 years, recruited from 28 secondary schools in Hong Kong. Four validated scales were used to assess family processes. Results showed that adolescents in non-intact families perceived relatively poorer family functioning, lower level of paternal and maternal behavioral control, lower level of paternal psychological control, and poorer parent–child relational qualities than did adolescents in intact families. This generally indicated that family processes were poorer in non-intact families, compared with those in intact families. The theoretical and practical implications of the findings were discussed.
Semantics-preserving and memory-efficient implementation of inter-task communication on static-priority or EDF schedulers

In previous work, we have proposed a method of preserving the functional semantics of model-based designs by the use of static checks and a double-buffer protocol [12]. However, this is restricted to static, fixed-priority scheduling and for high-priority to low-priority communications requires a double buffer to be stored for each pair of communicating tasks. In this paper we extend the method to dynamic-priority scheduling in the form of earliest-deadline-first (EDF) scheduling and show that, although scheduling is dynamic, a static buffering scheme can still be used. We also suggest some memory optimizations of our protocol which still preserve the original functional semantics. Finally, we show how model checking can be used to prove correctness of the scheme.
Enhancing the output power of GaN-based LEDs grown on wet-etched patterned sapphire substrates

GaN-based light-emitting diodes (LEDs) with emitting wavelength of 450 nm were grown on patterned sapphire substrates (PSSs) fabricated by chemical wet etching. The crystallography-etched facet was {1-102} R-plane with a 57deg against {0001} C-axis and had superior capability for enhancing light extraction efficiency. The light output power of the PSS LED was 1.15 times higher than that of the conventional LED at an injection current of 20 mA. The output power and external quantum efficiency were estimated to be 9 mW and 16.4%, respectively. The improvement was attributed not only to geometrical shapes of {1-102} crystallography-etched facets that efficiently scatter the guided light to find escape cones, but also to dislocation density reduction by adopting the PSS growth scheme
Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION)

Superparamagnetic iron oxide nanoparticles (SPION) are being widely used for various biomedical applications, for example, magnetic resonance imaging, targeted delivery of drugs or genes, and in hyperthermia. Although, the potential benefits of SPION are considerable, there is a distinct need to identify any potential cellular damage associated with these nanoparticles. Besides focussing on cytotoxicity, the most commonly used determinant of toxicity as a result of exposure to SPION, this review also mentions the importance of studying the subtle cellular alterations in the form of DNA damage and oxidative stress. We review current studies and discuss how SPION, with or without different surface coating, may cause cellular perturbations including modulation of actin cytoskeleton, alteration in gene expression profiles, disturbance in iron homeostasis and altered cellular responses such as activation of signalling pathways and impairment of cell cycle regulation. The importance of protein–SPION interaction and various safety considerations relating to SPION exposure are also addressed.
Effects of Cold Air on Cardiovascular Disease Risk Factors in Rat

The purpose of this study is to explore possible potential implications of cold air in cardiovascular disease (CVD) risk in rats. Healthy Wistar rats were exposed to artificial cold air under laboratory conditions, and their systolic blood pressure, heart rate, vasoconstriction, CVD risk factors, and myocardial damage indicators after cold air exposure were determined and evaluated. Systolic blood pressure, whole blood viscosity, and plasma level of norepinephrine, angiotensinⅡ, low density lipoprotein, total cholesterol, and fibrinogen in treatment groups increased significantly compared with control groups. No significant variations were found in plasma Mb and cTnT and myocardial tissue between the treatment and control groups. Results indicate that: (1) higher levels of SBP, WBV and LDL/HDL, total cholesterol (TC), and FG in blood may indicate higher CVD risks during cold air exposure; (2) cold air may exert continuous impacts on SBP and other CVD risk factors.
Fisetin Suppresses Macrophage-Mediated Inflammatory Responses by Blockade of Src and Syk

Flavonoids, such as fisetin (3,7,3′,4′-tetrahydroxyflavone), are plant secondary metabolites. It has been reported that fisetin is able to perform numerous pharmacological roles including anti-inflammatory, anti-microbial, and anti-cancer activities; however, the exact anti-inflammatory mechanism of fisetin is not understood. In this study, the pharmacological action modes of fisetin in lipopolysaccharide (LPS)-stimulated macrophage-like cells were elucidated by using immunoblotting analysis, kinase assays, and an overexpression strategy. Fisetin diminished the release of nitric oxide (NO) and reduced the mRNA levels of inducible NO synthase (iNOS), tumor necrosis factor (TNF)-α, and cyclooxygenase (COX)-2 in LPS-stimulated RAW264.7 cells without displaying cytotoxicity. This compound also blocked the nuclear translocation of p65/nuclear factor (NF)-κB. In agreement, the upstream phosphorylation events for NF-κB activation, composed of Src, Syk, and IκBα, were also reduced by fisetin. The phospho-Src level, triggered by overexpression of wild-type Src, was also inhibited by fisetin. Therefore, these results strongly suggest that fisetin can be considered a bioactive immunomodulatory compound with anti-inflammatory properties through suppression of Src and Syk activities.
Durability of GFRP Bars’ Bond to Concrete under Different Loading and Environmental Conditions

In the last decade, noncorrodible fiber-reinforced polymer (FRP) reinforcing bars have been increasingly used as the main reinforcement for concrete structures in harsh environments. Also, owing to their lower cost compared with other types of FRP bars, glass-FRP (GFRP) bars are more attractive to the construction industry, especially for implementation in bridge deck slabs. In North America, bridge deck slabs are exposed to severe environmental conditions, such as freeze-thaw action, in addition to traffic fatigue loads. Although the bond strength of GFRP bars has been proved to be satisfactory, their durability performance under the dual effects of fatigue-type loading and freeze-thaw action is still not well understood. Few experimental test data are available on the bond characteristics of FRP bars in concrete elements under different loading and environmental conditions. This research investigates the individual and combined effects of freeze-thaw cycles along with sustained axial load and fatigue loading on the bond characteristics of GFRP bars embedded in concrete. An FRP-reinforced concrete specimen was developed to apply axial-tension fatigue or sustained loads to GFRP bars within a concrete environment. A total of thirty-six test specimens was constructed and tested. The test parameters included bar diameter, concrete cover thickness, loading scheme, and environmental conditioning. After conditioning, each specimen was sectioned into two halves for pullout testing. Test results showed that fatigue load cycles resulted in approximately 50% loss in the bond strength of sand-coated GFRP bars to concrete, while freeze-thaw cycles enhanced their bond to concrete by approximately 40%. Larger concrete covers were found more important in cases of larger bar sizes simultaneously subjected to fatigue load and freeze-thaw cycles.
Origin of “current-onset voltage” variability in scaled MOSFETs

Present work analyzes the cause of “current-onset voltage” variability, which has been newly found to largely affect drain current variability [1]. It is found by 3D device simulation that the “current-onset voltage” variability is determined by how largely the channel potential fluctuates by random dopant disposition. Reducing RDF will suppress both threshold voltage and current-onset voltage variability as well.
Inhibition of NF‐kappaB/Rel induces apoptosis of murine B cells.

Apoptosis of the WEHI 231 immature B cell lymphoma line following membrane interaction with an antibody against the surface IgM chains (anti‐IgM) is preceded by dramatic changes in Nuclear Factor‐kappaB (NF‐kappaB)/ Rel binding activities. An early transient increase in NF‐kappaB/Rel binding is followed by a significant decrease in intensity below basal levels. Here we have explored the role of these changes in Rel‐related factors in B cell apoptosis. Treatment of WEH1 231 cells with N‐tosyl‐L‐phenylalanine chloromethyl ketone (TPCK), a protease inhibitor which prevents degradation of the inhibitor of NF‐kappaB (IkappaB)‐alpha, or with low doses of pyrrolidinedithiocarbamate (PDTC) selectively inhibited NF‐kappaB/Rel factor binding and induced apoptosis. Bcl‐XL expression protected WEHI 231 cells from apoptosis induced by these agents. Microinjection of WEHI 231 cells with either IkappaB‐alpha‐GST protein or a c‐Rel affinity‐purified antibody induced apoptosis. Ectopic c‐Rel expression ablated apoptosis induced by TPCK or anti‐IgM. Treatment of BALENLM 17 and A20 B lymphoma cells or normal murine splenic B lymphocytes with either TPCK or PDTC also resulted in apoptosis. These findings indicate that the drop in NF‐kappaB/Rel binding following anti‐IgM treatment activates apoptosis of WEHI 231 cells; furthermore, they implicate the NF‐kappaB/Rel family in control of apoptosis of normal and transformed B cells.