Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact on Diabetes Mellitus on the Epicardial Coronary Flow Velocity Assessed by the Thrombolysis in Myocardial Infarction Frame Count

The aim of this study is to evaluate the effect of type 2 diabetes mellitus on epicardial coronary flow velocity assessed by the thrombolysis in myocardial infarction frame count. The thrombolysis in myocardial infarction frame count was measured in 272 coronary arteries from 101 patients with type 2 diabetes mellitus and in 271 coronary arteries from 104 age- and gender-matched patients without type 2 diabetes mellitus referred for coronary angiography. The thrombolysis in myocardial infarction frame count was measured only in normal arteries or in arteries without significant lesion. By both univariate and multivariate analysis, the thrombolysis in myocardial infarction frame count was not related with either type 2 diabetes mellitus or the duration and glycated hemoglobin levels in the patients with type 2 diabetes mellitus. The thrombolysis in myocardial infarction frame count was significantly associated with body surface area, heart rate, and proximal coronary artery diameter. Type 2 diabetes mellitus did not affect epicardial coronary flow velocity assessed by the thrombolysis in myocardial infarction frame count.
Manual vs. computer-assisted sperm analysis: can CASA replace manual assessment of human semen in clinical practice?

OBJECTIVES The aim of the study was to check the quality of computer-assisted sperm analysis (CASA) system in comparison to the reference manual method as well as standardization of the computer-assisted semen assessment. MATERIAL AND METHODS The study was conducted between January and June 2015 at the Andrology Laboratory of the Division of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, Poland. The study group consisted of 230 men who gave sperm samples for the first time in our center as part of an infertility investigation. The samples underwent manual and computer-assisted assessment of concentration, motility and morphology. A total of 184 samples were examined twice: manually, according to the 2010 WHO recommendations, and with CASA, using the program set-tings provided by the manufacturer. Additionally, 46 samples underwent two manual analyses and two computer-assisted analyses. The p-value of p < 0.05 was considered as statistically significant. RESULTS Statistically significant differences were found between all of the investigated sperm parameters, except for non-progressive motility, measured with CASA and manually. In the group of patients where all analyses with each method were performed twice on the same sample we found no significant differences between both assessments of the same probe, neither in the samples analyzed manually nor with CASA, although standard deviation was higher in the CASA group. CONCLUSIONS Our results suggest that computer-assisted sperm analysis requires further improvement for a wider application in clinical practice.
Rapid Dissociation of Human Apurinic Endonuclease (Ape1) from Incised DNA Induced by Magnesium*

Repair of apurinic/apyrimidinic (AP) sites is initiated by AP endonucleases, such as the human Ape1 protein (also called Hap1, Apex, and Ref1). This and related enzymes show strong dependence on divalent cations, particularly magnesium. Here we explore the role of this metal in different stages of the Ape1 reaction: substrate binding, cleavage, and product release. We examined DNA binding using an electrophoretic approach and DNA cleavage in single-turnover and steady-state reactions. Magnesium at low to moderate concentrations accelerated both substrate and product release by wild-type Ape1 protein. For a mutant Ape1 protein with an aspartate to alanine substitution at residue 308, substrate in preformed protein-DNA complexes was more efficiently cleaved before release in contrast to wild-type Ape1, whereas product release was accelerated dramatically. The magnesium dependence of steady-state AP endonuclease reactions was sigmoidal for both wild-type and the aspartate 308 to alanine protein but was not sigmoidal for an aspartate 283 to alanine derivative of Ape1. These results show that magnesium affects both DNA interactions with and phosphodiester cleavage by Ape1 and can change the rate-limiting step of the reaction. Structural studies will need to be interpreted in the context of these diverse effects of the metal.
A Fault-Tolerant Two-Motor Drive With FCS-MP-Based Flux and Torque Control

Independently controlled multimotor drives are typically realized by using a common dc link and independent sets of three-phase inverters and motors. In the case of an open-circuit fault in an inverter leg, one motor becomes single phase. To enable continued controllable operation by eliminating single phasing, the supply for the motor phase with the faulted inverter leg can be paralleled to a healthy leg of another inverter using hardware reconfiguration. Hence, the two motors are now supplied from a five-leg inverter, which has inherent voltage and current limitations. Theoretically, violating the voltage limit leads to inverter overmodulation and large torque oscillations. It is shown here that the finite-control-set model predictive control, designed to control the machines' stator flux and torque, can consider the inherent voltage limit dynamically in the control loop. Apart from preserving the independent control of the two machines, the additional constraint consideration significantly widens the operating speed ranges of the machines. In particular, it is shown that, whenever the voltage limit is entered, the controller reduces the stator flux level automatically, without requiring external flux reference change. The obtained performance is illustrated using experimental results and is also compared to the conventional two-motor field-oriented control scheme. The control concept is thus fully experimentally verified.
Exercise promotes the expression of brain derived neurotrophic factor (BDNF) through the action of the ketone body β-hydroxybutyrate

Exercise induces beneficial responses in the brain, which is accompanied by an increase in BDNF, a trophic factor associated with cognitive improvement and the alleviation of depression and anxiety. However, the exact mechanisms whereby physical exercise produces an induction in brain Bdnf gene expression are not well understood. While pharmacological doses of HDAC inhibitors exert positive effects on Bdnf gene transcription, the inhibitors represent small molecules that do not occur in vivo. Here, we report that an endogenous molecule released after exercise is capable of inducing key promoters of the Mus musculus Bdnf gene. The metabolite β-hydroxybutyrate, which increases after prolonged exercise, induces the activities of Bdnf promoters, particularly promoter I, which is activity-dependent. We have discovered that the action of β-hydroxybutyrate is specifically upon HDAC2 and HDAC3, which act upon selective Bdnf promoters. Moreover, the effects upon hippocampal Bdnf expression were observed after direct ventricular application of β-hydroxybutyrate. Electrophysiological measurements indicate that β-hydroxybutyrate causes an increase in neurotransmitter release, which is dependent upon the TrkB receptor. These results reveal an endogenous mechanism to explain how physical exercise leads to the induction of BDNF. DOI: http://dx.doi. org/10.7554/eLife.15092. 001
Cyclic stretching force-induced early apoptosis in human periodontal ligament cells.

OBJECTIVE Human periodontal ligament (PDL) cells occur changes in morphology and express relative protein by stretching force. However, whether stretching force, especially excessive stretching force, induces PDL cell apoptosis is not yet clearly understood. In the present study we investigated the relationship between early apoptosis and stretching force in human PDL cells in vitro. MATERIALS AND METHODS The human PDL cells were obtained from healthy premolars. After three to five passages, the cells were stretched by strain 1%, 10% and 20% for 30 min, 1 h, 6 h and 12 h, then early apoptosis were detected through annexin fluorescein isothiocyanate (V-FITC) binding by flow cytometry and confocal laser scanning microscopy. RESULTS The experiments indicated that human PDL apoptotic cells in the early stage increased in a time- and force-dependent manner in response to stretching strain within 6 h, and then apoptosis decreased at 12 h. Human PDL cells which stretched inclined parallel to each other and aligned their long axis perpendicular to the stretching force vector, but in the centre of the disc, cells showed minimal deformation and unidirectional alignment of PDL cells. CONCLUSION The overall results suggested that stretching force not only influenced morphology but also induced early apoptosis in human PDL cells.
EVALUATING TUNA MANAGEMENT IN THE EASTERN PACIFIC OCEAN

We answer four questions about tuna management in the eastern Pacific Ocean: What are the management objectives? Are they reasonable? Can we determine whether they have been achieved? Have they been achieved? There are explicit, over arching management objectives including keeping populations at levels that will permit maximum sustainable yields, consideration of the precautionary approach, and consideration of ecosystem consequences, but although reasonable, these objec tives are vague from a stock-assessment perspective. Objectives of individual partic ipants are unlikely to be the same and collectively make achieving the overarching objectives more difficult. For data-rich stocks, we can determine whether the yieldbased objectives have been achieved, but even these results have alternative inter pretations. Unfortunately, when data are limited, the management objectives can not be evaluated. In the eastern Pacific, species differ in achievement of objectives. Yield-based objectives are probably being achieved for yellowfin tuna—yields may increase if effort is reallocated among fishing methods—whereas for bigeye tuna, biomass levels are falling below that necessary for maximum sustainable yield. For skipjack, although the population level is healthy, restrictions designed to protect bigeye and yellowfin tuna prevent fishermen from achieving maximum sustainable yield. By-catch of other species in the tuna fisheries is a management concern. The status and management of large pelagic fish populations worldwide is caus
Deep Aesthetic Learning Estimating Photo Aesthetic Rating Using Deep Convolutional Neural Network

Deep Learning has been widely applied in several computer vision applications such as item recognition and image retrieval. In addition to identifying what a image contains, it is also a interesting problem to explore about whether the image is appealing or the composition of the items are highly rated aesthetically. There are multiple studies using hand-designed or datadriven model to quantify the metrics regarding to photo quality such as color distribution, composition, sharpness etc. Aesthetic rating is such a complicated and unknown function of human beings’ psychological responses to the pixels that deep networks are potentially better tools to achieve similar modeling and predictions. Existing large aesthetic rating data-set also enable the training good deep networks. In this work, I developed a convolution neural network dedicated for learning to model and predict photo ratings from pixels. Novel data augmentation scheme and further fine tuning regression from the learned features are the two main parts of the contributions in this work. Results demonstrate better prediction performance compared with existing solutions using either conventional engineered features or deep networks. In addition, the trained network is able to out-perform human beings in estimating the aesthetic rating of images. The resulting performance are summarized and more details of network design and tuning are further discussed. The trained networks are further quantified which also provides insights to the understanding of human being’s appreciation to art and beauty.
MAGE-A3-specific anticancer immunotherapy in the clinical practice

Antigen-specific immunotherapy may offer a unique approach to fight cancer. We have demonstrated that specific immunotherapeutic regimens involving recombinant melanoma antigen family A3 (MAGE-A3) and different immunostimulants exert clinical anticancer activity. In particular, the combination of recombinant MAGE-A3 and AS15, a multicomponent immunostimulant, was found to elicit robust antigen-specific immune responses.
A Survey About Prediction-Based Data Reduction in Wireless Sensor Networks

One of the main characteristics of Wireless Sensor Networks (WSNs) is the constrained energy resources of their wireless sensor nodes. Although this issue has been addressed in several works and received much attention over the years, the most recent advances pointed out that the energy harvesting and wireless charging techniques may offer means to overcome such a limitation. Consequently, an issue that had been put in second place now emerges: the low availability of spectrum resources. Because of it, the incorporation of the WSNs into the Internet of Things and the exponential growth of the latter may be hindered if no control over the data generation is taken. Alternatively, part of the sensed data can be predicted without triggering transmissions that could congest the wireless medium. In this work, we analyze and categorize existing prediction-based data reduction mechanisms that have been designed for WSNs. Our main contribution is a systematic procedure for selecting a scheme to make predictions in WSNs, based on WSNs’ constraints, characteristics of prediction methods, and monitored data. Finally, we conclude the article with a discussion about future challenges and open research directions in the use of prediction methods to support the WSNs’ growth.
First detection of Potosia opaca larva attacks on Phoenix dactylifera and Phoenix canariensis in morocco: focus on pests control strategies and soil quality of prospected palm groves

The present study illustrated the impact of Potosia opaca larva as devastating of two species Phoenix Canariensis and Phoenix dactylifera. We examined the possible relationship between the health status of palm trees and the soil quality of plantations. This study was performed on oases of Marrakech and Errachidia during the period of 2014-2016. The present study results showed that the larvae found belong to Potosia opaca var. cardui Gyllenhal. Chemical and mineral analyses showed that the level of organic matter was higher in soil of Marrakech palm grove (2.06-3.62%) than in Errachidia (1.51%). Levels of available phosphorus (6.98 ppm) and total nitrogen (0.05%) were very low in soils of Errachidia palm groves. K, Cl, Fe, Zn and Cu contents were high in the planting soils of P. Canariensis without exceeding the anomaly thresholds. Lead concentration was found to be low in the studied soils (< to 16 ppm). Phytopahtological analyses show the presence of saprophytic fungi in particular the Dimaties and genus Fusarium with a non-virulent strain. Finally, the present study proposed a strategy to control the larvae of Potosia Opaca to limit its risk of contamination of Moroccan oases.
Adaptive Acid Tolerance Response of Streptococcus sobrinus

ABSTRACT Streptococcus mutans and Streptococcus sobrinus are the bacteria most commonly associated with human dental caries. A major virulence attribute of these and other cariogenic bacteria is acid tolerance. The acid tolerance mechanisms of S. mutans have begun to be investigated in detail, including the adaptive acid tolerance response (ATR), but this is not the case for S. sobrinus. An analysis of the ATR of two S. sobrinus strains was conducted with cells grown to steady state in continuous chemostat cultures. Compared with cells grown at neutral pH, S. sobrinus cells grown at pH 5.0 showed an increased resistance to acid killing and were able to drive down the pH through glycolysis to lower values. Unlike what is found for S. mutans, the enhanced acid tolerance and glycolytic capacities of acid-adapted S. sobrinus were not due to increased F-ATPase activities. Interestingly though, S. sobrinus cells grown at pH 5.0 had twofold more glucose phosphoenolpyruvate:sugar phosphotransferase system (PTS) activity than cells grown at pH 7.0. In contrast, glucose PTS activity was actually higher in S. mutans grown at pH 7.0 than in cells grown at pH 5.0. Silver staining of two-dimensional gels of whole-cell lysates of S. sobrinus 6715 revealed that at least 9 proteins were up-regulated and 22 proteins were down-regulated in pH 5.0-grown cells compared with cells grown at pH 7.0. Our results demonstrate that S. sobrinus is capable of mounting an ATR but that there are critical differences between the mechanisms of acid adaptation used by S. sobrinus and S. mutans.
Joint acoustic modeling of triphones and trigraphemes by multi-task learning deep neural networks for low-resource speech recognition

It is well-known in machine learning that multitask learning (MTL) can help improve the generalization performance of singly learning tasks if the tasks being trained in parallel are related, especially when the amount of training data is relatively small. In this paper, we investigate the estimation of triphone acoustic models in parallel with the estimation of trigrapheme acoustic models under the MTL framework using deep neural network (DNN). As triphone modeling and trigrapheme modeling are highly related learning tasks, a better shared internal representation (the hidden layers) can be learned to improve their generalization performance. Experimental evaluation on three low-resource South African languages shows that triphone DNNs trained by the MTL approach perform significantly better than triphone DNNs that are trained by the single-task learning (STL) approach by ~3-13%. The MTL-DNN triphone models also outperform the ROVER result that combines a triphone STL-DNN and a trigrapheme STL-DNN.
Leptin Impairs Cardiovagal Baroreflex Function at the Level of the Solitary Tract Nucleus

Circulating leptin is elevated in some forms of obesity-related hypertension, associated with impaired baroreflex function. Leptin receptors are present on vagal afferent fibers and neurons within the solitary tract nucleus, providing an anatomic distribution consistent with baroreflex modulation. Although solitary tract nucleus microinjection of 144 fmol/60 nL of leptin had no significant effect on baroreflex sensitivity for control of the heart rate in urethane/chloralose-anesthetized Sprague-Dawley rats, 500 fmol of leptin impaired baroreflex sensitivity for bradycardia in response to increases in pressure (1.15±0.04 versus 0.52±0.12 ms/mm Hg; P<0.01). Transgenic ASrAOGEN rats with low brain angiotensinogen have an upregulation of the leptin receptor and p85 &agr; mRNA in the dorsal medulla relative to Sprague-Dawley rats. Consistent with these observations, the response to leptin was enhanced in ASrAOGEN rats, because both the 144-fmol (1.46±0.08 versus 0.75±0.10 ms/mm Hg; P<0.001) and 500-fmol (1.36±0.32 versus 0.44±0.06 ms/mm Hg; P<0.05) leptin microinjections impaired baroreflex sensitivity. At these doses, leptin microinjection had no effect on resting pressure, heart rate, or the tachycardic response to decreases in pressure in Sprague-Dawley or ASrAOGEN rats. Thus, exogenous leptin at sites within the solitary tract nucleus impairs the baroreflex sensitivity for bradycardia induced by increases in arterial pressure, consistent with a permissive role in mediating increases in arterial pressure. Baroreflex inhibition was enhanced in animals with evidence of increased leptin receptor and relevant signaling pathway mRNA.
Protection of telomeres by a conserved Stn1–Ten1 complex

Telomeres are specialized chromatin structures that protect chromosome ends. Critical among telomere proteins are those that bind the telomeric single-strand DNA (ssDNA) overhangs. These proteins are thought to differ among eukaryotes. Three interacting proteins (Cdc13, Stn1, and Ten1) associate with the telomeric overhang in budding yeast, a single protein known as Pot1 (protection of telomeres-1) performs this function in fission yeast, and a two-subunit complex consisting of POT1 and TPP1 associates with telomeric ssDNA in humans. Cdc13 and Pot1 have related oligonucleotide/oligosaccharide-binding fold (OB-fold) domains that bind the telomeric ssDNA overhang. Here we show that Schizosaccharomyces pombe has Stn1- and Ten1-like proteins that are essential for chromosome end protection. Stn1 orthologs exist in all species that have Pot1, whereas Ten1-like proteins can be found in all fungi. Fission yeast Stn1 and Ten1 localize at telomeres in a manner that correlates with the length of the ssDNA overhang, suggesting that they specifically associate with the telomeric ssDNA. Unlike in budding yeast, in which Cdc13, Stn1, and Ten1 all interact, fission yeast Stn1 and Ten1 associate with each other, but not with Pot1. Our findings suggest that two separate protein complexes are required for chromosome end protection in fission yeast. Structural profiling studies detect OB-fold domains in Stn1 and Ten1 orthologs, indicating that protection of telomeres by multiple proteins with OB-fold domains is conserved in eukaryotic evolution.
Molecular Cancer apeutics linical Development one Deacetylase Inhibition Attenuates Cell Growth with ociated Telomerase Inhibition in High-Grade Ther ldhood Brain Tumor Cells

Download rrant epigenetic regulation of gene expression contributes to tumor initiation and progression. Studies plethora of hematologic and solid tumors support the use of histone deacetylase inhibitors (HDACi) as anticancer agents. However, the mechanism of HDACi action with respect to the temporal order of d cellular events is unclear. The present study investigates the anticancer effects of the HDACi trichosin high-grade childhood brain tumor cells. Acute exposure to trichostatin A resulted in marked inhiof cell proliferation, an increase in the proportion of G2-M cells, activation of H2A.X, and subsequent ion of apoptosis in the majority of cell lines. These phenotypic effects were associated with abrogation merase activity and human telomerase reverse transcriptase downregulation in the majority of cell In contrast, no cytotoxicity was observed in primary ependymal cells with respect to cilia function. inhibition of histone deacetylases leads to antiproliferative and proapoptotic effects in childhood brain Thus, tumor cells, likely to involve altered chromatin regulation at the human telomerase reverse transcriptase promoter. Mol Cancer Ther; 9(9); 2568–81. ©2010 AACR.