Miracle-dz/UniFAR-FTUs huggingface dataset:
Older Persons, and Caregiver Burden and Satisfaction in Rural Family Context

The present study is aimed at assessing the burden among the caregivers of the elderly in rural families and to standardize and compare Burden Assessment Scale (BAS) and Caregivers' Reaction Assessment scale (CRA) in Indian context. A Sample of 300 (176 women and 124 men) caregivers of the elderly was selected using a systematic sampling method from three village panchayats of a predominantly rural mandal (Padmanabham) of Visakhapatnam district, Andhra Pradesh. An interview schedule covering basic socio-demographic data and the two measures i.e. BAS and CRA was used to collect data from the caregivers. Findings reveal that burden and satisfaction of care giving is associated with sex and age of caregiver, and family income. A large percentage of caregivers expressed satisfaction about the care-giving role played by them. However, only a small percentage of women, as compared to men, expressed satisfaction about their care-giving role probably because the major burden of caring the elderly was actually shouldered by women rather than by men. Similarly, more women caregivers reported tiredness and worsening health due to stress arising out of their care giving roles. Lastly, evidence showed that higher age and lower income was found to bring down the caregiver satisfaction significantly.
Observation of multiple folding pathways of beta-hairpin trpzip2 from independent continuous folding trajectories

MOTIVATION After 10-year investigations, the folding mechanisms of beta-hairpins are still under debate. Experiments strongly support zip-out pathway, while most simulations prefer the hydrophobic collapse model (including middle-out and zip-in pathways). In this article, we show that all pathways can occur during the folding of beta-hairpins but with different probabilities. The zip-out pathway is the most probable one. This is in agreement with the experimental results. We came to our conclusions by 38 100-ns room-temperature all-atom molecular dynamics simulations of the beta-hairpin trpzip2. Our results may help to clarify the inconsistencies in the current pictures of beta-hairpin folding mechanisms.
Vertebral Coplanar Alignment: A Standardized Technique for Three Dimensional Correction in Scoliosis Surgery: Technical Description and Preliminary Results in Lenke Type 1 Curves

Study Design. Prospective multicentric study. Objective. To present the preliminary results of an innovative method for standardized correction of scoliosis, vertebral coplanar alignment (VCA), based on a novel concept: the relocation of vertebral axis in a single plane. Summary of Background Data. Normal standing spine has no rotation in coronal or transverse planes, therefore X and Z axis of vertebrae are in the same plane: they are coplanar. VCA intends to relocate these axis in one plane, correcting rotation and translation, while X axis are returned to its normal posterior divergence in sagittal plane in thoracic spine. Methods. Twenty-five consecutive adolescent idiopathic scoliosis patients (Lenke type 1) underwent posterior surgery with segmental pedicle screw fixation. Slotted tubes were attached to convex side screws. Two longitudinal rods were inserted through the end of tubes. Then, they were separated along the slots, driving the tubes into one plane, making the axis of the vertebrae coplanar and thus correcting transverse rotation and coronal translation. To obtain kyphosis, distal ends of the tubes were spread in thoracic spine. Correction was maintained by locking a definitive rod in the concave side, then tubes were retrieved and the convex side rod, inserted and tightened. Correction was assessed on preoperative and postoperative full-spine standing radiograph. Vertebral rotation was measured on computed tomography-scan and magnetic resonance imaging. Results. Preoperative average thoracic curves of 61° were corrected to 16° (73%). Preoperative average thoracolumbar curves of 39° were corrected to 12° (70%). Preoperative average thoracic apical rotation of 24° was corrected to 11° (56%). Preoperative average thoracic kyphosis of 18° remained unchanged after surgery; however, no patients had kyphosis <10° after surgery. Rib hump improved from 30 to 11 mm (65%). There were no perioperative complications. Conclusion. VCA provided excellent correction of coronal and transverse planes with normalization of thoracic kyphosis in Lenke type 1 adolescent idiopathic scoliosis surgery.
Meta-analysis of pain relief effects by laser irradiation on joint areas.

BACKGROUND Laser therapy has been proposed as a physical therapy for musculoskeletal disorders and has attained popularity because no side effects have been reported after treatment. However, its true effectiveness is still controversial because several clinical trials have reported the ineffectiveness of lasers in treating pain. METHODS In this systematic review, we investigate the clinical effectiveness of low-level laser therapy (LLLT) on joint pain. Clinical trials on joint pain satisfying the following conditions are included: the laser is irradiated on the joint area, the PEDro scale score is at least 5, and the effectiveness of the trial is measured using a visual analogue scale (VAS). To estimate the overall effectiveness of all included clinical trials, a mean weighted difference in change of pain on VAS was used. RESULTS MEDLINE is the main source of the literature search. After the literature search, 22 trials related to joint pain were selected. The average methodological quality score of the 22 trials consisting of 1014 patients was 7.96 on the PEDro scale; 11 trials reported positive effects and 11 trials reported negative effects. The mean weighted difference in change of pain on VAS was 13.96 mm (95% CI, 7.24-20.69) in favor of the active LLLT groups. When we only considered the clinical trials in which the energy dose was within the dose range suggested in the review by Bjordal et al. in 2003 and in World Association for Laser Therapy (WALT) dose recommendation, the mean effect sizes were 19.88 and 21.05 mm in favor of the true LLLT groups, respectively. CONCLUSIONS The review shows that laser therapy on the joint reduces pain in patients. Moreover, when we restrict the energy doses of the laser therapy into the dose window suggested in the previous study, we can expect more reliable pain relief treatments.
Organisational diagnoses: a survey of the literature and proposition of a new diagnostic model

Organisational diagnosis is a technique used to check an organisation's current health. A complete diagnosis not only checks the current health, but also suggests corrective measures. Diagnostic models proposed by many others were analysed in this paper and shortcomings in these approaches to organisational health diagnosis were identified. We therefore propose in this paper an alternative, concise yet concrete model for organisational diagnosis.
Mechanisms of Pinometostat (EPZ-5676) Treatment–Emergent Resistance in MLL-Rearranged Leukemia

DOT1L is a protein methyltransferase involved in the development and maintenance of MLL-rearranged (MLL-r) leukemia through its ectopic methylation of histones associated with well-characterized leukemic genes. Pinometostat (EPZ-5676), a selective inhibitor of DOT1L, is in clinical development in relapsed/refractory acute leukemia patients harboring rearrangements of the MLL gene. The observation of responses and subsequent relapses in the adult trial treating MLL-r patients motivated preclinical investigations into potential mechanisms of pinometostat treatment-emergent resistance (TER) in cell lines confirmed to have MLL-r. TER was achieved in five MLL-r cell lines, KOPN-8, MOLM-13, MV4-11, NOMO-1, and SEM. Two of the cell lines, KOPN-8 and NOMO-1, were thoroughly characterized to understand the mechanisms involved in pinometostat resistance. Unlike many other targeted therapies, resistance does not appear to be achieved through drug-induced selection of mutations of the target itself. Instead, we identified both drug efflux transporter dependent and independent mechanisms of resistance to pinometostat. In KOPN-8 TER cells, increased expression of the drug efflux transporter ABCB1 (P-glycoprotein, MDR1) was the primary mechanism of drug resistance. In contrast, resistance in NOMO-1 cells occurs through a mechanism other than upregulation of a specific efflux pump. RNA-seq analysis performed on both parental and resistant KOPN-8 and NOMO-1 cell lines supported two unique candidate pathway mechanisms that may explain the pinometostat resistance observed in these cell lines. These results are the first demonstration of TER models of the DOT1L inhibitor pinometostat and may provide useful tools for investigating clinical resistance. Mol Cancer Ther; 16(8); 1669–79. ©2017 AACR.
Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity

Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) infection is associated with a high case-fatality rate, and the potential pandemic spread of the virus is a public health concern. The spike protein of MERS-CoV (MERS-S) facilitates viral entry into host cells, which depends on activation of MERS-S by cellular proteases. Proteolytic activation of MERS-S during viral uptake into target cells has been demonstrated. However, it is unclear whether MERS-S is also cleaved during S protein synthesis in infected cells and whether cleavage is required for MERS-CoV infectivity. Here, we show that MERS-S is processed by proprotein convertases in MERS-S–transfected and MERS-CoV–infected cells and that several RXXR motifs located at the border between the surface and transmembrane subunit of MERS-S are required for efficient proteolysis. However, blockade of proprotein convertases did not impact MERS-S–dependent transduction of target cells expressing high amounts of the viral receptor, DPP4, and did not modulate MERS-CoV infectivity. These results show that MERS-S is a substrate for proprotein convertases and demonstrate that processing by these enzymes is dispensable for S protein activation. Efforts to inhibit MERS-CoV infection by targeting host cell proteases should therefore focus on enzymes that process MERS-S during viral uptake into target cells.
Prediction of Individual Brain Maturity Using fMRI

Connectivity Map of the Brain The growing appreciation that clinically abnormal behaviors in children and adolescents may be influenced or perhaps even initiated by developmental miscues has stoked an interest in mapping normal human brain maturation. Several groups have documented changes in gray and white matter using structural and functional magnetic resonance imaging (fMRI) in cross-sectional and longitudinal studies. Dosenbach et al. (p. 1358) developed an index of resting-state functional connectivity (that is, how tightly neuronal activities in distinct brain regions are correlated while the subject is at rest or even asleep) from analyses of three independent data sets (each based on fMRI scans of 150 to 200 individuals from ages 6 to 35 years old). Long-range connections increased with age and short-range connections decreased, indicating that networks become sparser and sharper with brain maturation. Multivariate pattern analysis of 5-minute brain scans provides a measure of brain maturity. Group functional connectivity magnetic resonance imaging (fcMRI) studies have documented reliable changes in human functional brain maturity over development. Here we show that support vector machine-based multivariate pattern analysis extracts sufficient information from fcMRI data to make accurate predictions about individuals’ brain maturity across development. The use of only 5 minutes of resting-state fcMRI data from 238 scans of typically developing volunteers (ages 7 to 30 years) allowed prediction of individual brain maturity as a functional connectivity maturation index. The resultant functional maturation curve accounted for 55% of the sample variance and followed a nonlinear asymptotic growth curve shape. The greatest relative contribution to predicting individual brain maturity was made by the weakening of short-range functional connections between the adult brain’s major functional networks.
Evaluation of the pathological response and prognosis following neoadjuvant chemotherapy in molecular subtypes of breast cancer

Background The pathological complete response of neoadjuvant chemotherapy for breast cancer correlates with the prognosis for survival. Tumors may have different prognoses according to their molecular subtypes. This study was performed to evaluate the relevance of the pathological response and prognosis following neoadjuvant chemotherapy in the molecular subtypes of breast cancer. Methods A consecutive series of 88 patients with operable breast cancer treated with neoadjuvant chemotherapy was analyzed. Patients were classified into four molecular subtypes based on the immunohistochemistry profile of the estrogen receptor, progesterone receptor, HER2, and Ki-67. The histological response was assessed according to Miller-Payne grading (MPG) and Residual Disease in Breast and Nodes (RDBN). Results Ten patients (11.4%) achieved a pathological complete response, assessed according to RDBN. The pathological complete response rate was 13.6% according to MPG. Patients with the triple-negative subtype were more likely to achieve a pathological complete response than those with luminal A breast cancer (P=0.03). MPG and RDBN are independent predictors of distant disease-free survival and local recurrence-free survival, but do not predict overall survival. Ki-67, size of invasive carcinoma, lymph nodes, molecular subtypes, MPG, and RDBN are important predictors of distant disease-free survival, local recurrence-free survival, and overall survival. Conclusion MPG and RDBN were similarly related to the patient’s prognosis. MPG was more suitable for evaluation of distant disease-free survival, and RDBN was more suitable for evaluation of local recurrence-free survival. Survival following neoadjuvant chemotherapy correlated with the pathological reaction rather than the molecular subtype of breast cancer. The molecular subtype of breast cancer was not correlated with pathological response in patients who did not achieve a pathological complete response.
HIV/AIDS Policy in Africa: What Has Worked in Uganda and What Has Failed in Botswana

A comparison of HIV|AIDS policies in Botswana and Uganda is revealing. It helps to highlight the kinds of policies that are necessary to come to terms with the pandemic in Africa, where it is already a public health disaster. It is argued that the promotion of condoms at an early stage proved to be counter-productive in Botswana, whereas the lack of condom promotion during the 1980s and early 1990s contributed to the relative success of behaviour change strategies in Uganda. Other important factors included national and local-level leadership, the engagement (or alienation) of religious groups and local healers and, most controversially, procedures of social compliance. We end with a call for more draconian measures than are currently envisaged. Copyright © 2004 John Wiley & Sons, Ltd.
Comparing the immunoregulatory effects of stem cells from human exfoliated deciduous teeth and bone marrow-derived mesenchymal stem cells.

Stem cells from human exfoliated deciduous teeth (SHED) have been introduced recently and possess characteristics similar to mesenchymal stem cells (MSCs). Because of their convenient accessibility and safety of harvest, SHED can be a preferable source for the ever-increasing MSCs' applications While they are new, their immunoproperties have not been adequately studied. In this study, we aimed to explore the effect of SHED on T lymphocytes and compare it to conventional MSCs (BMMSCs). At first the isolated T lymphocytes were activated specifically/nonspecifically in vitro and cocultured with SHED or BMMSCs under the same conditions, subsequently their proliferation and cytokine secretion (IL-2 and IFN-γ) were measured.In our experiment, BMMSCs and SHED inhibit the proliferation and cytokine production of both PHA and alloantigen stimulated T lymphocytes in a dose-dependent manner. In direct and indirect contact to T lymphocytes, the inhibition of BMMSCs (but not of SHED) was significantly different The cytokine production from activated T cells was affected differently by two types of MSCs. The inhibition decreased by the separation of lymphocytes and MSCs by a semipermeable membrane, but it was not abolished.This study showed that SHED suppress the activation of human T lymphocytes in vitro like other MSCs. Compared to BMMSCs, this suppression was alleviated. In the equal conditions, the pattern of immune-modulation of BMMSCs and SHED was different, suggesting that SHED do not exert the exact mechanisms of BMMSCs' immunosuppression. , This finding should be verified by further studies focused on the detailed mechanisms of the immunomodulation of SHED and also BMMSCs.
Parallel Interleaver Design for a High throughput HSPA+/LTE Multi-Standard Turbo Decoder

To meet the evolving data rate requirements of emerging wireless communication technologies, many parallel architectures have been proposed to implement high throughput turbo decoders. However, concurrent memory reading/writing in parallel turbo decoding architectures leads to severe memory conflict problem, which has become a major bottleneck for high throughput turbo decoders. In this paper, we propose a flexible and efficient VLSI architecture to solve the memory conflict problem for highly parallel turbo decoders targeting multi-standard 3G/4G wireless communication systems. To demonstrate the effectiveness of the proposed parallel interleaver architecture, we implemented an HSPA +/LTE/LTE-Advanced multi-standard turbo decoder with a 45 nm CMOS technology. The implemented turbo decoder consists of 16 Radix-4 MAP decoder cores, and the chip core area is 2.43 mm 2. When clocked at 600 MHz, this turbo decoder can achieve a maximum decoding throughput of 826 Mbps in the HSPA+ mode and 1.67 Gbps in the LTE/LTE-Advanced mode, exceeding the peak data rate requirements for both standards.
Heterostructured III-V Nanowires with Mixed Crystal Phases Grown by Au-assisted Molecular Beam Epitaxy

Nanowires (NWs) with diameters in the range of few 10s of nanometers and lengths up to few micrometers long are one-dimensional materials that have attracted a great deal of interest recently in various fields, including photonics, electronics and medicine. These onedimensional materials can offer improvement in the performance of optical and electronic devices due to their size-induced quantum confinement effects. In addition, their high surface area to volume ratio makes them potential candidates for various sensor applications. Several review articles reporting about the properties and applications of NWs
Tracking multiple humans in complex situations

Tracking multiple humans in complex situations is challenging. The difficulties are tackled with appropriate knowledge in the form of various models in our approach. Human motion is decomposed into its global motion and limb motion. In the first part, we show how multiple human objects are segmented and their global motions are tracked in 3D using ellipsoid human shape models. Experiments show that it successfully applies to the cases where a small number of people move together, have occlusion, and cast shadow or reflection. In the second part, we estimate the modes (e.g., walking, running, standing) of the locomotion and 3D body postures by making inference in a prior locomotion model. Camera model and ground plane assumptions provide geometric constraints in both parts. Robust results are shown on some difficult sequences.
Can we meet the challenge? The educating systems librarian research project report 3

The investigators examined 133 job advertisements with job title “systems librarians’‘ and other similar job titles in American Libraries from January 1996 to December 1997. Information about full‐time positions available in the United States only was collected. For examination, four variables and their components were studied: degree requirements, work experience, job responsibilities, and knowledge and skills. Based on the classified job advertisements three types of libraries were identified: academic, public and other libraries. Two follow‐up questionnaires were distributed to library directors and newly hired systems librarians of the 133 libraries. The follow‐up survey based on the two questionnaires reflects that the majority of employers are satisfied with their newly hired systems librarians. However, they have higher expectations from the positions in terms of qualifications and responsibilities. The investigators also examined the curricula of seven American LIS schools which concentrate on educating “systems librarians” to provide recommendations to the curriculum design.
Oral distribution of Candida species and presence of oral lesions in Brazilian leprosy patients under multidrug therapy.

OBJECTIVE The aim of this study was to evaluate the prevalence of Candida spp. and presence of oral lesions in Brazilian leprosy patients under multidrug therapy (MDT). METHODS Thirty-eight individuals (18 males and 20 females, median age 53 years) clinically and microbiologically diagnosed as leprosy (lepromatous variant), and under MDT for at least 45 days were studied. The control group constituted by 38 healthy individuals (median age 53.5), matched to the test group in relation to age, gender and oral conditions. Oral rinses were collected and the Candida identification was performed by phenotypic tests. The existence of Candida dubliniensis among the isolates was analyzed using a validated multiplex PCR assay. Twenty-nine leprosy patients were examined intra-orally for the presence of lesions. Data were analyzed by z- and Mann-Whitney tests (alpha = 5%). RESULTS Yeast carriage rate between leprosy patients (65.8%) and controls (47.4%) was similar (P = 0.099), and no significant difference between yeast counts was observed (P = 0.1004). Candida albicans was the most frequently isolated species in both groups. In the leprosy group, Candida tropicalis and Candida parapsilosis were also identified. In the control group, we additionally identified Candida tropicalis, Candida glabrata and Candida kefyr. Candida dubliniensis was not detected. No leprosy-related oral lesion was registered. CONCLUSION Within the limits of the study, we concluded that Brazilian leprosy patients under MDT showed similar levels of carriage and Candida species distribution in relation to the controls.
ProTox: a web server for the in silico prediction of rodent oral toxicity

Animal trials are currently the major method for determining the possible toxic effects of drug candidates and cosmetics. In silico prediction methods represent an alternative approach and aim to rationalize the preclinical drug development, thus enabling the reduction of the associated time, costs and animal experiments. Here, we present ProTox, a web server for the prediction of rodent oral toxicity. The prediction method is based on the analysis of the similarity of compounds with known median lethal doses (LD50) and incorporates the identification of toxic fragments, therefore representing a novel approach in toxicity prediction. In addition, the web server includes an indication of possible toxicity targets which is based on an in-house collection of protein–ligand-based pharmacophore models (‘toxicophores’) for targets associated with adverse drug reactions. The ProTox web server is open to all users and can be accessed without registration at: http://tox.charite. de/tox. The only requirement for the prediction is the two-dimensional structure of the input compounds. All ProTox methods have been evaluated based on a diverse external validation set and displayed strong performance (sensitivity, specificity and precision of 76, 95 and 75%, respectively) and superiority over other toxicity prediction tools, indicating their possible applicability for other compound classes.