Miracle-dz/UniFAR-FTUs huggingface dataset:
Inducible MHC Class II Expression by Mast Cells Supports Effector and Regulatory T Cell Activation 1

In addition to their well-established role as regulators of allergic response, recent evidence supports a role for mast cells in influencing the outcome of physiologic and pathologic T cell responses. One mechanism by which mast cells (MCs) influence T cell function is indirectly through secretion of various cytokines. It remains unclear, however, whether MCs can directly activate T cells through Ag presentation, as the expression of MHC class II by MCs has been controversial. In this report, we demonstrate that in vitro stimulation of mouse MCs with LPS and IFN-γ induces the expression of MHC class II and costimulatory molecules. Although freshly isolated peritoneal MCs do not express MHC class II, an in vivo inflammatory stimulus increases the number of MHC class II-positive MCs in situ. Expression of MHC class II granted MCs the ability to process and present Ags directly to T cells with preferential expansion of Ag-specific regulatory T cells over naive T cells. These data support the notion that, in the appropriate setting, MCs may regulate T cell responses through the direct presentation of Ag.
Aflatoxin and viral hepatitis exposures in Guatemala: Molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence

Liver cancer is an emerging global health issue, with rising incidence in both the United States and the economically developing world. Although Guatemala experiences the highest rates of this disease in the Western hemisphere and a unique 1:1 distribution in men and women, few studies have focused on this population. Thus, we determined the prevalence and correlates of aflatoxin B1 (AFB1) exposure and hepatitis virus infection in Guatemalan adults. Healthy men and women aged ≥40 years (n = 461), residing in five departments of Guatemala, were enrolled in a cross-sectional study from May—October of 2016. Serum AFB1-albumin adducts were quantified using isotope dilution mass spectrometry. Multivariate linear regression was used to assess relationships between AFB1-albumin adduct levels and demographic factors. Biomarkers of hepatitis B virus and hepatitis C virus infection were assessed by immunoassay and analyzed by Fisher’s exact test. AFB1-albumin adducts were detected in 100% of participants, with a median of 8.4 pg/mg albumin (range, 0.2–814.8). Exposure was significantly higher (p<0.05) in male, rural, low-income, and less-educated participants than in female, urban, and higher socioeconomic status participants. Hepatitis B and C seropositivity was low (0.9% and 0.5%, respectively). Substantial AFB1 exposure exists in Guatemalan adults, concurrent with low prevalence of hepatitis virus seropositivity. Quantitatively, AFB1 exposures are similar to those previously found to increase risk for liver cancer in Asia and Africa. Mitigation of AFB1 exposure may reduce liver cancer incidence and mortality in Guatemala, warranting further investigation.
Saccharomyces cerevisiae strains for second-generation ethanol production: from academic exploration to industrial implementation

Abstract The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. After a discussion of the challenges that these novel industrial contexts impose on yeast strains, this minireview describes key metabolic engineering strategies that have been developed to address these challenges. Additionally, it outlines how proof-of-concept studies, often developed in academic settings, can be used for the development of robust strain platforms that meet the requirements for industrial application. Fermentation performance of current engineered industrial S. cerevisiae strains is no longer a bottleneck in efforts to achieve the projected outputs of the first large-scale second-generation ethanol plants. Academic and industrial yeast research will continue to strengthen the economic value position of second-generation ethanol production by further improving fermentation kinetics, product yield and cellular robustness under process conditions.
Individual Differences in Risky Decision-Making Among Seniors Reflect Increased Reward Sensitivity

Increasing age is associated with subtle but meaningful changes in decision-making. It is unknown, however, to what degree these psychological changes are reflective of age-related changes in decision quality. Here, we investigated the effect of age on latent cognitive processes associated with risky decision-making on the Balloon Analog Risk Task (BART). In the BART, participants repetitively inflate a balloon in order to increase potential reward. At any point, participants can decide to cash-out to harvest the reward, or they can continue, risking a balloon pop that erases all earnings. We found that among seniors, increasing age was associated with greater reward-related risk taking when the balloon has a higher probability of popping (i.e., a “high risk” condition). Cognitive modeling results from hierarchical Bayesian estimation suggested that performance differences were due to increased reward sensitivity in high risk conditions in seniors.
Cardiovascular Imaging Techniques in Systemic Rheumatic Diseases

The risk of cardiovascular (CV) events and mortality is significantly higher in patients with systemic rheumatic diseases than in the general population. Although CV involvement in such patients is highly heterogeneous and may affect various structures of the heart, it can now be diagnosed earlier and promptly treated. Various types of assessments are employed for the evaluation of CV risk such as transthoracic or transesophageal echocardiography, magnetic resonance imaging (MRI), and computed tomography (CT) to investigate valve abnormalities, pericardial disease, and ventricular wall motion defects. The diameter of coronary arteries can be assessed using invasive quantitative coronarography or intravascular ultrasound, and coronary flow reserve can be assessed using non-invasive transesophageal or transthoracic ultrasonography (US), MRI, CT, or positron emission tomography (PET) after endothelium-dependent vasodilation. Finally, peripheral circulation can be measured invasively using strain-gauge plethysmography in an arm after the arterial infusion of an endothelium-dependent vasodilator or non-invasively by means of US or MRI measurements of flow-mediated vasodilation of the brachial artery. All of the above are reliable methods of investigating CV involvement, but more recently, introduced use of speckle tracking echocardiography and 3-dimensional US are diagnostically more accurate.
openXBOW - Introducing the Passau Open-Source Crossmodal Bag-of-Words Toolkit

We introduce openXBOW, an open-source toolkit for the generation of bag-of-words (BoW) representations from multimodal input. In the BoW principle, word histograms were first used as features in document classification, but the idea was and can easily be adapted to, e.g., acoustic or visual low-level descriptors, introducing a prior step of vector quantisation. The openXBOW toolkit supports arbitrary numeric input features and text input and concatenates computed subbags to a final bag. It provides a variety of extensions and options. To our knowledge, openXBOW is the first publicly available toolkit for the generation of crossmodal bags-of-words. The capabilities of the tool are exemplified in two sample scenarios: time-continuous speech-based emotion recognition and sentiment analysis in tweets where improved results over other feature representation forms were observed.
Quantitative Trait Transcripts Mapping Coupled with Expression Quantitative Trait Loci Mapping Reveal the Molecular Network Regulating the Apetalous Characteristic in Brassica napus L.

The apetalous trait of rapeseed (Brassica napus, AACC, 2n = 38) is important for breeding an ideal high-yield rapeseed with superior klendusity to Sclerotinia sclerotiorum. Currently, the molecular mechanism underlying the apetalous trait of rapeseed is unclear. In this study, 14 petal regulators genes were chosen as target genes (TGs), and the expression patterns of the 14 TGs in the AH population, containing 189 recombinant inbred lines derived from a cross between apetalous “APL01” and normal “Holly,” were analyzed in two environments using qRT-PCR. Phenotypic data of petalous degree (PDgr) in the AH population were obtained from the two environments. Both quantitative trait transcript (QTT)-association mapping and expression QTL (eQTL) analyses of TGs expression levels were performed to reveal regulatory relationships among TGs and PDgr. QTT mapping for PDgr determined that PLURIPETALA (PLP) was the major negative QTT associated with PDgr in both environments, suggesting that PLP negatively regulates the petal development of line “APL01. ” The QTT mapping of PLP expression levels showed that CHROMATIN-REMODELING PROTEIN 11 (CHR11) was positively associated with PLP expression, indicating that CHR11 acts as a positive regulator of PLP expression. Similarly, QTT mapping for the remaining TGs identified 38 QTTs, associated with 13 TGs, and 31 QTTs, associated with 10 TGs, respectively, in the first and second environments. Additionally, eQTL analyses of TG expression levels showed that 12 and 11 unconditional eQTLs were detected in the first and second environment, respectively. Based on the QTTs and unconditional eQTLs detected, we presented a hypothetical molecular regulatory network in which 14 petal regulators potentially regulated the apetalous trait in “APL01” through the CHR11-PLP pathway. PLP acts directly as the terminal signal integrator negatively regulating petal development in the CHR11-PLP pathway. These findings will aid in the understanding the molecular mechanism underlying the apetalous trait of rapeseed.
Correction : An alternative surgical approach reduces variability following filament induction of experimental stroke in mice

Animal models are essential for understanding the pathology of stroke and investigating potential treatments. However, in vivo stroke models are associated, particularly in mice, with high variability in lesion volume. We investigated whether a surgical refinement where reperfusion is not reliant on the Circle of Willis reduced outcome variability. Mice underwent 60 min of transient middle cerebral artery occlusion avoiding ligation of the external carotid artery. During reperfusion, the common carotid artery was either ligated (standard approach), or it was repaired to allow re-establishment of blood flow through the common carotid artery. All mice underwent MRI scanning for assessment of infarct volume, apparent diffusion coefficient and fractional anisotropy, along with terminal assessment of infarct volume by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Repairing the common carotid artery following middle cerebral artery occlusion enhanced reperfusion (P<0.01) and reduced the variability seen in both total (histological analysis, P=0.008; T2-weighted MRI, P=0.015) and core (diffusion tensor MRI, P=0.043) lesion volume. Avoiding external carotid artery ligation may improve animal wellbeing, through reduced weight loss, while using an alternative surgical approach that enabled reperfusion through the common carotid artery decreased the variability in lesion volume
Interpersonal trauma moderates the relationship between personality factors and suicidality of individuals with posttraumatic stress disorder

Individuals with posttraumatic stress disorder (PTSD) are more prone to suicidal ideation and behavior. While those who have experienced interpersonal trauma exhibit more suicidality than those who have experienced non-interpersonal trauma, it is unclear how the traumatic effects are related to an individual’s personality characteristics. This study examined the association between interpersonal trauma and personality factors with suicidality, and elucidated the moderating role of interpersonal trauma in individuals with PTSD. The study included 6,022 participants from the Korean Epidemiologic Catchment Area Study 2011. The Korean Version of Composite International Diagnostic Interview was used for the survey, including the participants’ history of suicidality, the traumas they have experienced, and their PTSD symptoms. The 11-item version of the Big Five Inventory (BFI-11) was used to assess the participants’ personality factors. 76 individuals were diagnosed with PTSD, while 810 had been exposed to trauma but were not diagnosed with any DSM-IV mental disorder. Among the individuals with PTSD, those who had experienced interpersonal trauma were more likely to have suicidal ideation than those who had experienced non-interpersonal trauma (p = .020; odds ratio [OR] = 3.643; 95% confidence interval of OR = [1.226, 10.825]). High agreeableness and conscientiousness predicted less suicidality in those exposed to non-interpersonal trauma, while predicting more suicidality in those exposed to interpersonal trauma. Clinicians examining individuals with PTSD should pay closer attention to the trauma that they have experienced, as well as their personality factors, to provide appropriate treatment.
Type 2 diabetes epidemic in East Asia: a 35–year systematic trend analysis

Facing the challenge of effective prevention type 2 diabetes (T2DM) in China (as part of global health) requires knowledge about both the temporal trend and risk factors variation in T2DM. We searched the PubMed, CNKI, WANFANG, and International Diabetes Federation (IDF) databases for data on the prevalence of T2DM/ IGT (impaired glucose tolerance) published from January 1, 1980 to December 31, 2014 in China, Japan and Korea. The prevalence of T2DM was estimated with 95% confidence intervals (CIs) using random–effects meta–analysis. T2DM prevalence trend in the next 10 years was estimated by using a time series regression model based on the 35 years of data. The 621 articles covered 11.8 million Chinese people, 1.64 million Japanese, and 37.69 million Koreans. The aggregate prevalence of T2DM in China has increased sharply from 1.3% in 1980–1989 to 4.5% in 1990–1999, 6.8% 2000–2009, and 8.7% in 2010–2014. We estimated that by 2025, T2DM prevalence will have grown to 12.5%. Central obesity is the largest preventable cause of T2DM. We also found that female having a very high BMI (body mass index, ≥28 kg/m2) and being an older (≥50 years old) female are next–highest risk factors for T2DM compared with male. Consistent with the patterns characterized for China, T2DM prevalence in Japan increased with aging, and men were more likely to develop T2DM. It was the same as Korea. In the Far East, especially in China, T2DM prevalence will continue to increase until 2025. Statistical analyses were conducted using Stata 12.0 and SPSS 19.0.
1 Organized for Parliament ? Explaining the Electoral Success of Radical Right Parties in Post-Communist Europe

Over the last three decades a great deal of research has been carried out in an attempt to explain the electoral performance of radical right parties in Europe. Most approaches concentrate on demand side determinants and have some limitations. We compensated for these shortcomings and focus on the context of party competition and supply-side determinants (consistency of ideological discourse, functioning party propaganda, the continuity of leader in office, and strong party organization) to explain the electoral success of radical right parties in post-communist Europe. We conducted our analysis at party level on nine radical right parties in four countries from Central and Eastern Europe (Bulgaria, the Czech Republic, Hungary, and Romania) between 1990 and 2014. The bivariate and multivariate (ordinal logistic regression) analyses draw on unique data collected from primary and secondary sources.
Ultrasonic computed tomography based on full-waveform inversion for bone quantitative imaging

We introduce an ultrasonic quantitative imaging method for long bones based on full-waveform inversion. The cost function is defined as the difference in the L2-norm sense between observed data and synthetic results at a given iteration of the iterative inversion process. For simplicity, and in order to reduce the computational cost, we use a two-dimensional acoustic approximation. The inverse problem is solved iteratively based on a quasi-Newton technique called the Limited-memory Broyden–Fletcher–Goldfarb–Shanno method. We show how the technique can be made to work fine for benchmark models consisting of a single cylinder, and then five cylinders, the latter case including significant multiple diffraction effects. We then show pictures obtained for a tibia-fibula bone pair model. Convergence is fast, typically in 15 to 30 iterations in practice in each frequency band used. We discuss the so-called ‘cycle skipping’ effect that can occur in such full waveform inversion techniques and make them remain trapped in a local minimum of the cost function. We illustrate strategies that can be used in practice to avoid this. Future work should include viscoelastic materials rather than acoustic, and real data instead of synthetic data.
For Black men, being tall increases threat stereotyping and police stops

Significance Young Black men are stereotyped as threatening, which can have grave consequences for interactions with police. We show that these threat stereotypes are even greater for tall Black men, who face greater discrimination from police officers and elicit stronger judgments of threat. We challenge the assumption that height is intrinsically good for men. White men may benefit from height, but Black men may not. More broadly, we demonstrate how demographic factors (e.g., race) can influence how people interpret physical traits (e.g., height). This difference in interpretation is a matter not of magnitude but of meaning: The same trait is positive for some groups of people but negative for others. Height seems beneficial for men in terms of salaries and success; however, past research on height examines only White men. For Black men, height may be more costly than beneficial, primarily signaling threat rather than competence. Three studies reveal the downsides of height in Black men. Study 1 analyzes over 1 million New York Police Department stop-and-frisk encounters and finds that tall Black men are especially likely to receive unjustified attention from police. Then, studies 2 and 3 experimentally demonstrate a causal link between perceptions of height and perceptions of threat for Black men, particularly for perceivers who endorse stereotypes that Black people are more threatening than White people. Together, these data reveal that height is sometimes a liability for Black men, particularly in contexts in which threat is salient.
Foursquare to the Rescue: Predicting Ambulance Calls Across Geographies

Understanding how ambulance incidents are spatially distributed can shed light to the epidemiological dynamics of geographic areas and inform healthcare policy design. Here we analyze a longitudinal dataset of more than four million ambulance calls across a region of twelve million residents in the North West of England. With the aim to explain geographic variations in ambulance call frequencies, we employ a wide range of data layers including open government datasets describing population demographics and socio-economic characteristics, as well as geographic activity in online services such as Foursquare. Working at a fine level of spatial granularity we demonstrate that daytime population levels and the deprivation status of an area are the most important variables when it comes to predicting the volume of ambulance calls at an area. Foursquare check-ins on the other hand complement these government sourced indicators, offering a novel view to population nightlife and commercial activity locally. We demonstrate how check-in activity can provide an edge when predicting certain types of emergency incidents in a multi-variate regression model.
The Drosophila junctophilin gene is functionally equivalent to its four mammalian counterparts and is a modifier of a Huntingtin poly-Q expansion and the Notch pathway

ABSTRACT Members of the Junctophilin (JPH) protein family have emerged as key actors in all excitable cells, with crucial implications for human pathophysiology. In mammals, this family consists of four members (JPH1-JPH4) that are differentially expressed throughout excitable cells. The analysis of knockout mice lacking JPH subtypes has demonstrated their essential contribution to physiological functions in skeletal and cardiac muscles and in neurons. Moreover, mutations in the human JPH2 gene are associated with hypertrophic and dilated cardiomyopathies; mutations in JPH3 are responsible for the neurodegenerative Huntington's disease-like-2 (HDL2), whereas JPH1 acts as a genetic modifier in Charcot–Marie–Tooth 2K peripheral neuropathy. Drosophila melanogaster has a single junctophilin (jp) gene, as is the case in all invertebrates, which might retain equivalent functions of the four homologous JPH genes present in mammalian genomes. Therefore, owing to the lack of putatively redundant genes, a jp Drosophila model could provide an excellent platform to model the Junctophilin-related diseases, to discover the ancestral functions of the JPH proteins and to reveal new pathways. By up- and downregulation of Jp in a tissue-specific manner in Drosophila, we show that altering its levels of expression produces a phenotypic spectrum characterized by muscular deficits, dilated cardiomyopathy and neuronal alterations. Importantly, our study has demonstrated that Jp modifies the neuronal degeneration in a Drosophila model of Huntington's disease, and it has allowed us to uncover an unsuspected functional relationship with the Notch pathway. Therefore, this Drosophila model has revealed new aspects of Junctophilin function that can be relevant for the disease mechanisms of their human counterparts. Summary: This work reveals that the Drosophila Junctophilin protein has similar functions to its mammalian homologues and uncovers new interactions of potential biomedical interest with Huntingtin and Notch signalling.
The case against ergocalciferol (vitamin D 2 ) as a vitamin supplement 1,2

Supplemental vitamin D is available in 2 distinct forms: ergocalciferol (vitamin D 2 ) and cholecalciferol (vitamin D 3 ). Pharmacopoeias haveofficiallyregardedthese2formsasequivalentandinterchange-able,yetthispresumptionofequivalenceisbasedonstudiesofricketspreventionininfantsconducted70yago.Theemergenceof25-hydroxyvitaminDasameasureofvitaminDstatusprovidesanobjective,quantitativemeasureofthebiologicalresponsetovitaminDadministration.Asaresult,vitaminD 3 has proven to be the more potent form of vitamin D in all primate species, including humans. Despite an emerging body of evidence suggesting several plausible explanations for the greater bioefficacy of vitamin D 3 , the form of vitamin D used in major preparations of prescriptions in North America is vitamin D 2 . The case that vitamin D 2 should no longer be considered equivalent to vitamin D 3 is based on differences in their efficacy at raising serum 25-hydroxyvitamin D, diminished binding of vitamin D 2 metabolites to vitamin D binding protein in plasma, and a nonphysiologic metabolism and shorter shelf life of vitamin D 2 . Vitamin D 2 , or ergocalciferol, should not be regarded as a nutrient suitable for supplementation or fortification.