Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of supplementation with purified red clover (Trifolium pratense) isoflavones on plasma lipids and insulin resistance in healthy premenopausal women

Consumption of isoflavone-rich soyabean protein is reported to reduce total and LDL-cholesterol, but the specific components responsible are undetermined. In a previous crossover trial we showed that purified isoflavones, derived from red clover (Trifolium pratense), raised HDL3-cholesterol in premenopausal women; however, these findings were inconclusive due to period and carryover effects. In an attempt to overcome this problem, we utilised a parallel study designed to re-examine the effects of purified isoflavones on plasma lipoproteins and markers of insulin resistance in premenopausal women. Twenty-five healthy premenopausal women participated in a double-blind, randomised, parallel study. The treatment group (n 12) consumed a placebo for the first menstrual cycle and an isoflavone supplement (86 mg/d, derived from red clover) for three cycles, while the placebo group (n 13) consumed a placebo supplement for four menstrual cycles. Blood samples were collected weekly during cycles 1, 3 and 4. Supplementation with isoflavones resulted in a 15-fold increase in urinary isoflavone excretion (P<0·0001). There were no significant effects on total cholesterol, LDL- and HDL-cholesterol, HDL subfractions, triacylglycerol, lipoprotein(a), glucose or insulin concentrations. Our present results indicate that purified isoflavones derived from red clover have no effect on cholesterol homeostasis or insulin resistance in premenopausal women, a group which is at low risk of CHD.
Factors Associated With Diabetes-Specific Health-Related Quality of Life in Youth With Type 1 Diabetes: The Global TEENs Study

OBJECTIVE Our objective was to characterize diabetes-specific health-related quality of life (D-HRQOL) in a global sample of youth and young adults with type 1 diabetes (T1D) and to identify the main factors associated with quality of life. RESEARCH DESIGN AND METHODS The TEENs study was an international, cross-sectional study of youth, 8–25 years of age, with T1D. Participants (N = 5,887) were seen in clinical sites in 20 countries across 5 continents enrolled for 3 predetermined age groups: 8–12, 13–18, and 19–25 years of age. To assess D-HRQOL, participants completed the PedsQL Diabetes Module 3.0 and were interviewed about family-related factors. Specifics about treatment regimen and self-management behaviors were collected from medical records. RESULTS Across all age groups, females reported significantly lower D-HRQOL than did males. The 19–25-year age group reported the lowest D-HRQOL. Multivariate linear regression analyses revealed that D-HRQOL was significantly related to HbA1c; the lower the HbA1c, the better the D-HRQOL. Three diabetes-management behaviors were significantly related to better D-HRQOL: advanced methods used to measure food intake; more frequent daily blood glucose monitoring; and more days per week that youth had ≥30 min of physical activity. CONCLUSIONS In all three age groups, the lower the HbA1c, the better the D-HRQOL, underscoring the strong association between better D-HRQOL and optimal glycemic control in a global sample of youth and young adults. Three diabetes-management behaviors were also related to optimal glycemic control, which represent potentially modifiable factors for clinical interventions to improve D-HRQOL as well as glycemic control.
Ultra-Short Versus Conventional Uncemented Stems for Hip Replacement in Octogenarians.

The aim of this study was to determine how an ultra-short anatomic uncemented stem vs a diaphyseal filling five-eighths porous coated uncemented femoral stem (conventional stem) affects the survival of primary total hip arthroplasties in octogenarians. The current study consisted of 52 patients (55 hips) in the ultra-short stem group (mean age, 85.5±5.3 years) and 61 patients (72 hips) in the diaphyseal (conventional) stem group (mean age, 84.1±4.8 years). The predominant diagnosis was osteoarthritis, followed by osteonecrosis of the femoral head, in both groups. Average follow-up was 6.1 years (range, 5-8 years) in the ultra-short stem group and 6.3 years (range, 5-9 years) in the conventional stem group. Pre- (41 vs 39 points) and postoperative (85 vs 86 points) Harris hip scores were similar between groups (P=.131 and .129, respectively). The incidence of thigh pain was 0% (0 of 52 patients) in the ultra-short stem group and 15% (9 of 61 patients) in the conventional stem group. The revision rate was 1.8% (1 hip) in the ultra-short stem group and 1.4% (1 hip) in the conventional stem group. Survivorship of the femoral stem was 98.2% in the ultra-short stem group at 6.1 years and 98.6% in the conventional stem group at 6.3 years. Ultra-short anatomic and diaphyseal filling uncemented stems obtained osseointegration in all hips. However, the prevalence of thigh pain and periprosthetic fracture were significantly higher in the patients with a conventional stem than in those with an ultra-short stem. [Orthopedics. 2018; 41(1):28-34. ].
Enterococcus faecalis Modulates Immune Activation and Slows Healing During Wound Infection

Enterococcus faecalis is one of most frequently isolated bacterial species in wounds yet little is known about its pathogenic mechanisms in this setting. Here, we used a mouse wound excisional model to characterize the infection dynamics of E. faecalis and show that infected wounds result in two different states depending on the initial inoculum. Low dose inocula were associated with short term, low titer colonization whereas high dose inocula were associated with acute bacterial replication and long term persistence. High dose infection and persistence were also associated with immune cell infiltration, despite suppression of some inflammatory cytokines and delayed wound healing. During high dose infection, the multiple peptide resistance factor (MprF) which is involved in resisting immune clearance, contributes to E. faecalis fitness. These results comprehensively describe a mouse model for investigating E. faecalis wound infection determinants, and suggest that both immune modulation and resistance contribute to persistent, non-healing wounds.
Sickle cell anemia mice develop a unique cardiomyopathy with restrictive physiology

Significance Sickle cell anemia (SCA) is a common monogenic disorder associated with significant morbidity and mortality and a high incidence of unexplained sudden death in young adults. With the prevention of infections, there is an increasing appreciation for cardiopulmonary complications and a cardiac phenotype that cannot be solely attributed to chronic anemia. We used mouse models of SCA and iron-deficient anemia to show distinct functional, pathological, ultrastructural, and molecular cardiac features causing a unique restrictive cardiomyopathy in SCA that predisposed the myocardium to electrophysiological abnormalities and sudden death. This is a comprehensive longitudinal analysis in preclinical mouse models that unifies the previously reported diverse cardiac phenotypes in SCA, and opens new avenues for early diagnostics and targeted therapies for human SCA-related cardiac disease. Cardiopulmonary complications are the leading cause of mortality in sickle cell anemia (SCA). Elevated tricuspid regurgitant jet velocity, pulmonary hypertension, diastolic, and autonomic dysfunction have all been described, but a unifying pathophysiology and mechanism explaining the poor prognosis and propensity to sudden death has been elusive. Herein, SCA mice underwent a longitudinal comprehensive cardiac analysis, combining state-of-the-art cardiac imaging with electrocardiography, histopathology, and molecular analysis to determine the basis of cardiac dysfunction. We show that in SCA mice, anemia-induced hyperdynamic physiology was gradually superimposed with restrictive physiology, characterized by progressive left atrial enlargement and diastolic dysfunction with preserved systolic function. This phenomenon was absent in WT mice with experimentally induced chronic anemia of similar degree and duration. Restrictive physiology was associated with microscopic cardiomyocyte loss and secondary fibrosis detectable as increased extracellular volume by cardiac-MRI. Ultrastructural mitochondrial changes were consistent with severe chronic hypoxia/ischemia and sarcomere diastolic-length was shortened. Transcriptome analysis revealed up-regulation of genes involving angiogenesis, extracellular-matrix, circadian-rhythm, oxidative stress, and hypoxia, whereas ion-channel transport and cardiac conduction were down-regulated. Indeed, progressive corrected QT prolongation, arrhythmias, and ischemic changes were noted in SCA mice before sudden death. Sudden cardiac death is common in humans with restrictive cardiomyopathies and long QT syndromes. Our findings may thus provide a unifying cardiac pathophysiology that explains the reported cardiac abnormalities and sudden death seen in humans with SCA.
Abstract: Surgical Site Infection in Immediate Breast Reconstruction: Does Chemotherapy Timing Make a Difference?

chemotherapy to surgery date noted between patients who developed a post-operative surgical site infection and those who did not (4.40 ± 1.58 vs 4.72 ± 1.39 weeks; p=0.517). CONCLUSION: In our study, chemotherapy timing did not increase the odds of surgical site infections in patients undergoing immediate breast reconstruction. a needed a Two one for
Combining Mineral and Polymer Binder Material Science for Sustainability in Construction and Restoration

Abstract The study of microstructure formation in polymer-cement concrete provides opportunities to exploit synergetic actions between cement and polymer, leading to performance improvement and to a wide range of new and innovative properties and applications. Polymers can reduce the impact of construction industry on environment, by decreasing the carbon footprint of cement and concrete production. Renovation and restoration, largely figuring in the concept of sustainable construction development, thank their growing share in construction activity to the input of polymers in repair and binder materials and in rehabilitation procedures. The study of ancient binders and mortars reveals aspects of the origins of the observed long lasting durability of those ancient mortars. It also reveals the interaction mechanisms between carbonation of air hardening components and hydration of hydraulic components, which in turn helps to develop chemical activation methods (i.e. alkaline activation) to improve the hydraulic properties of pozzolans and industrial residues to develop inorganic polymers (i.e. geopolymers) for eventually full replacement of cement in binders.
Systemic Inflammatory Response to Malaria During Pregnancy Is Associated With Pregnancy Loss and Preterm Delivery

Background Pregnancy malaria (PM) is associated with a proinflammatory immune response characterized by increased levels of cytokines and chemokines such as tumor necrosis factor-α, interferon-γ, interleukin 10 (IL-10), and CXCL9. These changes are associated with poor outcomes including low birthweight delivery and maternal anemia. However, it is unknown if inflammatory pathways during malaria are related to pregnancy loss and preterm delivery (PTD). Methods Cytokine and chemokine levels were measured in maternal peripheral blood at enrollment, gestational week 30-32, and delivery, and in placental blood, of 638 women during a longitudinal cohort study in Ouelessebougou, Mali. Plasmodium falciparum infection was assessed by blood smear microscopy at all visits. Results PM was associated with increased levels of cytokines and chemokines including IL-10 and CXCL9. In a competing risks model adjusted for known covariates, high CXCL9 levels measured in the peripheral blood during pregnancy were associated with increased risk of pregnancy loss and PTD. At delivery, high IL-10 levels in maternal blood were associated with an increase in pregnancy loss, and increased IL-1β levels in placental blood were associated with pregnancy loss and PTD. Conclusions PM is associated with increased proinflammatory cytokine and chemokine levels in placental and maternal peripheral blood. Systemic inflammatory responses to malaria during pregnancy predict increased risk of pregnancy loss and PTD. Clinical Trials Registration NCT01168271.
Breathing Disturbances Without Hypoxia Are Associated With Objective Sleepiness in Sleep Apnea

Study Objectives To determine whether defining two subtypes of sleep-disordered breathing (SDB) events-with or without hypoxia-results in measures that are more strongly associated with hypertension and sleepiness. Methods A total of 1022 participants with 2112 nocturnal polysomnograms from the Wisconsin Sleep Cohort were analyzed with our automated algorithm, developed to detect breathing disturbances and desaturations. Breathing events were time-locked to desaturations, resulting in two indices-desaturating (hypoxia-breathing disturbance index [H-BDI]) and nondesaturating (nonhypoxia-breathing disturbance index [NH-BDI]) events-regardless of arousals. Measures of subjective (Epworth Sleepiness Scale) and objective (2981 multiple sleep latency tests from a subset of 865 participants) sleepiness were analyzed, in addition to clinically relevant clinicodemographic variables. Hypertension was defined as BP ≥ 140/90 or antihypertensive use. Results H-BDI, but not NH-BDI, correlated strongly with SDB severity indices that included hypoxia (r ≥ 0.89, p ≤ .001 with 3% oxygen-desaturation index [ODI] and apnea hypopnea index with 4% desaturations). A doubling of desaturation-associated events was associated with hypertension prevalence, which was significant for ODI but not H-BDI (3% ODI OR = 1.06, 95% CI = 1.00-1.12, p < .05; H-BDI OR 1.04, 95% CI = 0.98-1.10) and daytime sleepiness (β = 0.20 Epworth Sleepiness Scale [ESS] score, p < .0001; β = -0.20 minutes in MSL on multiple sleep latency test [MSLT], p < .01). Independently, nondesaturating event doubling was associated with more objective sleepiness (β = -0.52 minutes in MSL on MSLT, p < .001), but had less association with subjective sleepiness (β = 0.12 ESS score, p = .10). In longitudinal analyses, baseline nondesaturating events were associated with worsening of H-BDI over a 4-year follow-up, suggesting evolution in severity. Conclusions In SDB, nondesaturating events are independently associated with objective daytime sleepiness, beyond the effect of desaturating events.
Energy Absorption Behavior of Carbon-Fiber-Reinforced Thermoplastic Composite Tubes

The primary objective of this work was to study the energy absorption characteristics of carbon-fiber-reinforced thermoplastic composite tubes. Composite tubes with different thermoplastic matrices-polyetheretherketone (PEEK), polyetherimide (PEI), polyimide (PI), and polyarylsulfone (PAS) -have been investigated. Fiber orientations of 0°,±5°±10°, ±15°, ±20°, ±25° and ±30° with respect to the axis of the tube were used. Among the thermoplastic tubes investigated, the carbon fiber/PEEK tubes displayed the highest specific energy absorption capability. This was attributed to the superior fracture toughness of the carbon fiber/PEEK composite material and a large number of frond splits and fractured fibers in the crush zone. The specific energy absorption capability was dependent on the fiber orientation. Efforts have been made to relate the change in specific energy to the variation of microfracture processes in the crush zone.
Global Ensemble Texture Representations are Critical to Rapid Scene Perception

Traditionally, recognizing the objects within a scene has been treated as a prerequisite to recognizing the scene itself. However, research now suggests that the ability to rapidly recognize visual scenes could be supported by global properties of the scene itself rather than the objects within the scene. Here, we argue for a particular instantiation of this view: That scenes are recognized by treating them as a global texture and processing the pattern of orientations and spatial frequencies across different areas of the scene without recognizing any objects. To test this model, we asked whether there is a link between how proficient individuals are at rapid scene perception and how proficiently they represent simple spatial patterns of orientation information (global ensemble texture). We find a significant and selective correlation between these tasks, suggesting a link between scene perception and spatial ensemble tasks but not nonspatial summary statistics In a second and third experiment, we additionally show that global ensemble texture information is not only associated with scene recognition, but that preserving only global ensemble texture information from scenes is sufficient to support rapid scene perception; however, preserving the same information is not sufficient for object recognition. Thus, global ensemble texture alone is sufficient to allow activation of scene representations but not object representations. Together, these results provide evidence for a view of scene recognition based on global ensemble texture rather than a view based purely on objects or on nonspatially localized global properties.
Impact Resistance Behaviour of Light Weight Rice Husk Concrete with Bamboo Reinforcement

This paper investigate the performance of lightweight rice husk concrete (LWRHC) with varied bamboo reinforcement content for the concrete slab of 300mm × 300mm size reinforced with varied slab thickness subjected to low impact projectile test. A self-fabricated drop-weight impact test rig with a steel ball weight of 1.236 kg drop at 0.65 m height has been used in this research work. The main variables for the study is to find the relationship of the impact resistance against the amount of bamboo reinforcement and slab thickness. A linear relationship has been established between first and ultimate crack resistance against bamboo diameters and slab thickness by the experiment. The linear relationship has also been established between the service (first) crack and ultimate crack resistance against the bamboo reinforcement diameter and slab thickness. 5% RH content exhibit better first and ultimate crack resistance up to 1.80 times and up to 1.72 times respectively against 10% RH content.
Preparation and characterization of carboxyl-treated multiwalled carbon nanotube-reinforced shape-memory polyurethane nanocomposites

Shape-memory polyurethane (SMPU) having segmented structure, with 4,4-diphenylmethane diisocyanate as hard segment, poly caprolactone diol 4000 as soft segment, and 1,4-butane diol as chain extender was synthesized using two-step polymerization technique. This pure SMPU was blended with unmodified and modified multiwalled nanotubes (MWNTs), using twin screw extruder and melt spinning technique. X-Ray diffraction, differential scanning calorimetry, morphological analysis, and shape-memory behavior results for the SMPUs are presented in this study. An improvement in the thermal stability and degradation temperature of the SMPU was observed especially in the soft segments, upon 0.1% loading of MWNT. Slight decrease in crystallinity was recorded for MWNT/SMPU as compared to virgin SMPU. The recovery ratio of SMPU fiber increased to 90% with 0.1 wt% loading of MWNT, but the spinnability decreased due to reduced crystallinity and viscosity with an addition of nanotubes to the polyurethane matrix.
Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4

Significance The kinase WNK4 (with-no-lysine kinase 4) is an important regulator of the Na-Cl cotransporter (NCC) in the renal distal convoluted tubule (DCT). Volume depletion induces angiotensin II, activating PKC, which prevents WNK4 degradation by phosphorylating the KLHL3/CUL3 ubiquitin ligase. We now show that PKC also directly phosphorylates WNK4 at multiple sites in cell culture. Phosphorylation of two of these sites, S64 and S1196, promotes increased WNK4 kinase activity by increasing autophosphorylation of the WNK4 T-loop at S332. Volume depletion also induces phosphorylation of WNK4-S64 in the DCT in vivo, promoting NCC activity. These findings provide insights into the mechanisms regulating activity of NCC and the promotion of renal Na-Cl reabsorption without concomitant K+ secretion in volume depletion. With-no-lysine kinase 4 (WNK4) regulates electrolyte homeostasis and blood pressure. WNK4 phosphorylates the kinases SPAK (Ste20-related proline alanine-rich kinase) and OSR1 (oxidative stress responsive kinase), which then phosphorylate and activate the renal Na-Cl cotransporter (NCC). WNK4 levels are regulated by binding to Kelch-like 3, targeting WNK4 for ubiquitylation and degradation. Phosphorylation of Kelch-like 3 by PKC or PKA downstream of AngII or vasopressin signaling, respectively, abrogates binding. We tested whether these pathways also affect WNK4 phosphorylation and activity. By tandem mass spectrometry and use of phosphosite-specific antibodies, we identified five WNK4 sites (S47, S64, S1169, S1180, S1196) that are phosphorylated downstream of AngII signaling in cultured cells and in vitro by PKC and PKA. Phosphorylation at S64 and S1196 promoted phosphorylation of the WNK4 kinase T-loop at S332, which is required for kinase activation, and increased phosphorylation of SPAK. Volume depletion induced phosphorylation of these sites in vivo, predominantly in the distal convoluted tubule. Thus, AngII, in addition to increasing WNK4 levels, also modulates WNK4 kinase activity via phosphorylation of sites outside the kinase domain.
Intensity-Specific Differential Leukocyte DNA Methylation in Physical (In)Activity: An Exploratory Approach

The aim of this exploratory study was to investigate how sedentary behavior (SB) and physical activity (PA) influence DNA methylation at a global, gene-specific, and health-related pathway level. SB, light PA (LPA), and moderate-to-vigorous PA (MVPA) were assessed objectively for 41 Flemish men using the SenseWear Pro 3 Armband. CpG site-specific methylation in leukocytes was determined using the Illumina HumanMethylation 450 BeadChip. Correlations were calculated between time spent on the three PA intensity levels and global DNA methylation, using a z-score-based method to determine global DNA methylation levels. To determine whether CpG site-specific methylation can be predicted by these three PA intensity levels, linear regression analyses were performed. Based on the significantly associated CpG sites at α = 0.005, lists were created including all genes with a promoter region overlapping these CpG sites. A biological pathway analysis determined to what extent these genes are overrepresented within several pathways. No significant associations were observed between global DNA methylation and SB (r = 0.084), LPA (r = -0.168), or MVPA (r = -0.125), although the direction of the correlation coefficients is opposite to what is generally reported in literature. SB has a different impact on global and gene-specific methylation than PA, but also LPA and MVPA affect separate genes and pathways. Furthermore, the function of a pathway seems to determine its association with SB, LPA, or MVPA. Multiple PA intensity levels, including SB, should be taken into account in future studies investigating the effect of physical (in)activity on human health through epigenetic mechanisms.