Miracle-dz/UniFAR-FTUs huggingface dataset:
Thermo-Plasmonic Killing of Escherichia coli TG1 Bacteria

Plasmonic photo-thermal therapy (PPTT) is a minimally invasive, drug-free, therapy based on the properties of noble metal nanoparticles, able to convert a bio-transparent electromagnetic radiation into heat. PPTT has been used against cancer and other diseases. Herein, we demonstrate an antimicrobial methodology based on the properties of gold nanorods (GNRs). Under a resonant laser irradiation GNRs become highly efficient light to heat nano-converters extremely useful for PPTT applications. The concept here is to assess the antimicrobial effect of easy to synthesize, suitably purified, water-dispersible GNRs on Escherichia coli bacteria. A control on the GNRs concentration used for the process has been demonstrated critical in order to rule out cytotoxic effects on the cells, and still to be able to generate, under a near infrared illumination, an adequate amount of heat suited to increase the temperature up to ≈50 °C in about 5 min. Viability experiments evidenced that the proposed system accomplished a killing efficiency suitable to reducing the Escherichia coli population of about 2 log CFU (colony-forming unit).
Incorporating dosimetric features into the prediction of 3D VMAT dose distributions using deep convolutional neural network

An accurate prediction of achievable dose distribution on a patient specific basis would greatly improve IMRT/VMAT planning in both efficiency and quality. Recently machine learning techniques have been proposed for IMRT dose prediction based on patient’s contour information from planning CT. In these existing prediction models geometric/anatomic features were learned for building the dose prediction models and few features that characterize the dosimetric properties of the patients were utilized. In this study we propose a method to incorporate the dosimetric features in the construction of a more reliable dose prediction model based on the deep convolutional neural network (CNN). In addition to the contour information, the dose distribution from a PTV-only plan (i.e. the plan with the best PTV coverage by sacrificing the OARs sparing) is also employed as the model input to build a deep learning based dose prediction model. A database of 60 volumetric modulated arc therapy (VMAT) plans for the prostate cancer patients was used for training. The trained prediction model was then tested on a cohort of ten cases. Dose difference maps, DVHs, dosimetric endpoints and statistical analysis of the sum of absolute residuals (SARs) were used to evaluate the proposed method. Our results showed that the mean SARs for the PTV, bladder and rectum using our method were 0.007 ± 0.003, 0.035 ± 0.032 and 0.067 ± 0.037 respectively, lower than the SARs obtained with the contours-based method, indicating the potential of the proposed approach in accurately predicting dose distribution.
Gender, hyperandrogenism and vitamin D deficiency related functional and morphological alterations of rat cerebral arteries

Hyperandrogenism is a risk factor of cerebrovascular diseases as androgens can alter markedly the regulation of cerebrovascular tone. We examined the combined impact of androgen excess and vitamin D deficiency (VDD), a common co-morbidity in hyperandrogenic disorders, on remodeling and testosterone-induced vascular responses of anterior cerebral arteries (ACA) in order to evaluate the interplay between androgens and VDD in the cerebral vasculature. Male and female Wistar rats were either fed with vitamin D deficient or vitamin D supplemented diet. Half of the female animals from both groups received transdermal testosterone treatment. After 8 weeks, vessel lumen, wall thickness and testosterone-induced vascular tone of isolated ACA were determined using pressure microangiometry and histological examination. Androgen receptor protein expression in the wall of cerebral arteries was examined using immunohistochemistry. In female rats only combined VDD and testosterone treatment decreased the lumen and increased the wall thickness of ACA. In males, however VDD by itself was able to decrease the lumen and increase the wall thickness. Vascular reactivity showed similar alterations: in females, testosterone constricted the ACA only after combined VDD and hyperandrogenism, whereas in males VDD resulted in increased testosterone-induced contractions in spite of decreased androgen receptor expression. In conclusion, a marked interplay between hyperandrogenism and VDD results in inward remodeling and enhanced testosterone-induced constrictions of cerebral arteries, which might compromise the cerebral circulation and thus, increase the risk of stroke in the long term. In addition, the early cerebrovascular manifestation of VDD appears to require androgen excess and thus, depends on gender.
The correlation between the microstructure of meibomian glands and ocular Demodex infestation

Abstract Meibomian gland dysfunction (MGD) is a common disease in ophthalmic clinic. This study aimed to explore ocular Demodex infestation on the microstructure changes of the meibomian glands (MGs) in patients with MGD by in vivo confocal microscopy (IVCM). We retrospectively reviewed 103 eyes of 52 patients with MGD and 62 eyes of 31 non-MGD patients. All enrolled patients underwent IVCM examination. The following IVCM parameters were recorded: the MG acinar density (MAD), MG acinar longest diameter (MALD), MG acinar shortest diameter (MASD), MG orifice area (MOA), severity of MG fibrosis (MF), MG acinar irregularity (MAI), meibum secretion reflectivity (MSR), inhomogeneous appearance of walls of acinar units (AWI) and periglandular interstices of acinar units (API), and the number of Demodex. The positive rate of Demodex infestation in MGDs was 89.32%, and statistically higher than control group (controls; P < .001). All parameters showed statistically significant differences between MGDs and controls (P < .001), and Demodex-negative group and Demodex-positive group (P < .05) in both MGDs and controls, except MAD (P = .826) in controls. The number of Demodex was positively correlated with MALD, MASD, MF, MAI, MSR, AWI, and API in MGDs and controls (P < .05), and negatively correlated with MAD and MOA in MGDs (P < .05). MOA showed a strong significant correlation with the number of Demodex in controls (P < .001), whereas there was no significant difference between the number of Demodex and the MAD in controls (P = .448). Demodex can cause microstructural changes of MGs, which can cause or aggravate MGD, and the more the number of Demodex infestation, the more serious the structural damage.
Development and Evaluation of a Novel HNB Based Isothermal Amplification Assay for Fast Detection of Pyrimethamine Resistance (S108N) in Plasmodium falciparum

Sulphadoxine and pyrimethamine (SP) have been used as long-acting partner antimalarial drugs in artemisinin combination therapy (ACT) for falciparum malaria. The emergence and increasing spread of SP resistance in malaria-endemic areas have become a challenge for the control of malaria. Therefore, regular monitoring of the mutation status of partner drugs is important for the better management of drug policy. There are limitations with traditional molecular methods and there is an urgent need for an easy method for diagnosis of drug resistance. In this study we have introduced and developed a novel single nucleotide polymorphism loop-mediated isothermal amplification (SNP–LAMP) approach based on a hydroxynaphthol blue (HNB) indicator for the easier and quicker detection of pyrimethamine resistance in Plasmodium falciparum malaria. To implement this novel approach, many sets of LAMP primers were designed and tested. Finally, one set of forward inner primer M1 (FIPM1) of LAMP primer was selected that specifically distinguishes pyrimethamine-resistant P. falciparum malaria. The LAMP reactions were optimized at 60–66 °C for 45 min. High sensitivity (7 parasites/µL) was observed with 10−4 fold dilutions (<2 ng DNA) of genomic DNA. Moreover, this approach has the potential to be applied even in laboratories unfamiliar with PCR or other molecular methods, and in future, this can be helpful for the better management of anti-malarial policy.
The sexual selection continuum

The evolution of mate choice for genetic benefits has become the tale of two hypotheses: Fisher's ‘run–away’ and ‘good genes’, or viability indicators. These hypotheses are often pitted against each other as alternatives, with evidence that attractive males sire more viable offspring interpreted as support for good genes and with a negative or null relationship between mating success of sons and other components of fitness interpreted as favouring the Fisher process. Here, we build a general model of female choice for indirect benefits that captures the essence of both the ‘Fisherian’ and ‘good–genes’ models. All versions of our model point to a single process that favours female preference for males siring offspring of high reproductive value. Enhanced mating success and survival are therefore equally valid genetic benefits of mate choice, but their relative importance varies depending on female choice costs. The relationship between male attractiveness and survival may be positive or negative, depending on life–history trade–offs and mating skew. This relationship can change sign in response to increased costliness of choice or environmental change. Any form of female preference is subject to self–reinforcing evolution, and any relationship (or lack thereof) between male display and offspring survival is inevitably an indicator of offspring reproductive values. Costly female choice can be maintained with or without higher offspring survival.
Role of Fatty Liver Index and Metabolic Factors in the Prediction of Nonalcoholic Fatty Liver Disease in a Lean Population Receiving Health Checkup

OBJECTIVES: Some metabolic factors and noninvasive markers, including fatty liver index (FLI), are used to predict nonalcoholic fatty liver disease (NAFLD) in obese patients. Despite the increasing prevalence of NAFLD in lean patients (lean-NAFLD), the risk factors and predictors are not well determined in this population. We investigated factors associated with lean-NAFLD and validated their predictive ability. METHODS: From 9,293 examinees who underwent routine health checkups, we enrolled 4,000, aged ≥20 years, with a body mass index <24 kg/m2 in our lean-NAFLD study population. NAFLD diagnoses were made according to the patients' histories, laboratory values, and sonographic criteria. Clinical variables, serum sugar, lipid, and liver profiles were evaluated using multiple logistic regression analysis. The predictive ability and optimal cutoff values for NAFLD were determined according to the area under the receiver operating characteristic curve. RESULTS: Overall, 18.5% (n = 740) of the lean population had NAFLD. Male sex, body mass index, body fat mass, fasting plasma glucose, uric acid, alanine aminotransferase, triglyceride, and FLI values were associated with NAFLD. FLI had the best discriminative ability to predict lean-NAFLD compared to the other biochemical markers. We further used the Youden index test and found an optimum cut-off value for FLI of 15 with the highest discriminant ability than other values. DISCUSSION: The prevalence of lean-NAFLD was not low. FLI was superior to other predictors including sex, liver function, and other metabolic factors, in the prediction of lean-NAFLD. FLI may be considered an easy to use, noninvasive marker to screen for lean-NAFLD.
Risk of Anxiety and Depression in Patients with Inflammatory Bowel Disease: A Nationwide, Population-Based Study

Background and Aims: Inflammatory bowel disease (IBD) may be associated with anxiety and depression. The aim of this study was to evaluate the incidence of anxiety and depression in patients with IBD compared to the general population. Methods: A nationwide population-based cohort study was conducted using claims data from the National Healthcare Insurance service in Korea. We compared the incidence of anxiety and depression between 15,569 IBD patients and 46,707 non-IBD controls, age and sex matched at a ratio of 1:3. Results: During a mean follow-up of six years, IBD patients experienced significantly more anxiety (12.2% vs. 8.7%; p < 0.001) and depression (8.0% vs. 4.7%; p < 0.001) compared to controls. The curves showing cumulative incidences of anxiety and depression showed a steep rise within one year following a diagnosis of IBD, leading to lines with a constant slope. The hazard ratio (HR) for new onset anxiety following a diagnosis of Crohn’s disease (CD) and ulcerative colitis (UC) was 1.63 and 1.60, respectively, compared to controls (p < 0.001). Compared to controls, the HR for developing depression after a diagnosis of CD and UC was 2.09 and 2.00, respectively (p < 0.001). The risks of anxiety and depression in patients with IBD were higher compared to controls, except in those with diabetes mellitus, hypertension, and dyslipidemia, or who required immunomodulators and biologics within one year of the IBD diagnosis. Conclusions: The risk of anxiety and depression increased after a diagnosis of IBD compared to the general population.
Dietary Patterns Are Related to Clinical Characteristics in Memory Clinic Patients with Subjective Cognitive Decline: The SCIENCe Project

As nutrition is one of the modifiable risk factors for cognitive decline, we studied the relationship between dietary quality and clinical characteristics in cognitively normal individuals with subjective cognitive decline (SCD). We included 165 SCD subjects (age: 64 ± 8 years; 45% female) from the SCIENCe project, a prospective memory clinic based cohort study on SCD. The Dutch Healthy Diet Food Frequency Questionnaire (DHD-FFQ) was used to assess adherence to Dutch guidelines on vegetable, fruit, fibers, fish, saturated fat, trans fatty acids, salt and alcohol intake (item score 0–10, higher score indicating better adherence). We measured global cognition (Mini Mental State Examination), cognitive complaints (Cognitive Change Index self-report; CCI) and depressive symptoms (Center for Epidemiologic Studies Depression Scale; CES-D). Using principal component analysis, we identified dietary components and investigated their relation to clinical characteristics using linear regression models adjusted for age, sex and education. We identified three dietary patterns: (i) “low-Fat-low-Salt”, (ii) “high-Veggy”, and (iii) “low-Alcohol-low-Fish”. Individuals with lower adherence on “low-Fat-low-Salt” had more depressive symptoms (β −0.18 (−2.27–−0.16)). Higher adherence to “high-Veggy” was associated with higher MMSE scores (β 0.30 (0.21–0.64)). No associations were found with the low-Alcohol-low-Fish component. We showed that in SCD subjects, dietary quality was related to clinically relevant outcomes. These findings could be useful to identify individuals that might benefit most from nutritional prevention strategies to optimize brain health.
Radio jet structures at ∼100 pc and larger scales of the γ-ray-emitting narrow-line Seyfert 1 galaxy PMN J0948+0022

The narrow-line Seyfert 1 (NLS1) galaxy PMN J0948+0022 is an archetype of γ-ray-emitting NLS1s in active galactic nuclei (AGNs). In this study, we investigate its radio structures using archival data obtained using the Karl G. Jansky Very Large Array (VLA) and the Very Long Baseline Array (VLBA). The new VLA images reveal an outermost radio emission separated by 9.1 arcsec. Its resolved structure and steep spectrum suggest a terminal shock in a radio lobe energized by the jet from the PMN J0948+0022 nucleus. This large-scale radio component is found at almost the same position angle as that of the pc-scale jet, indicating a stable jet direction up to ∼1 Mpc. Its apparent one-sidedness implies a moderate advancing speed (β > 0.27). The kinematic age of <1× 107 yr of the jet activity is consistent with the expected NLS1 phase of ∼107 yr in the AGN lifetime. The VLBA image reveals the jet structure at distances ranging from r = 0.82 milliarcsec to 3.5 milliarcsec, corresponding to approximately 100 pc, where superluminal motions were found. The jet width profile (∝ r1.12) and flux-density profile (∝ r−1.44) depending on the distance r along the jet suggest that the jet kinetic energy is converted to internal energy in this region. The jet is causally connected in a nearly conical streamline, which is supported by ambient pressure at 100 pc scales in the host galaxy of PMN J0948+0022.
TRPV2 Calcium Channel Gene Expression and Outcomes in Gastric Cancer Patients: A Clinically Relevant Association

Gastric cancer (GC) is characterized by poor efficacy and the modest clinical impact of current therapies. Apoptosis evasion represents a causative factor for treatment failure in GC as in other cancers. Since intracellular calcium homeostasis regulation has been found to be associated with apoptosis resistance, the aberrant expression of intracellular calcium regulator genes (CaRGs) could have a prognostic value in GC patients. We analyzed the association of the expression levels of 98 CaRGs with prognosis by the log-rank test in a collection of 1524 GC samples from four gene expression profiling datasets. We also evaluated differential gene expression in comparison with normal stomach tissue, and then we crossed results with tissue microarrays from the Human Protein Atlas. Among the investigated CaRGs, patients with high levels of TRPV2 expression were characterized by a shorter overall survival. TRPV2 expression was found to increase according to tumor stage. Both mRNA and protein levels were significantly higher in tumor than normal stomach samples. TRPV2 was also associated with poor prognosis in the Lauren’s intestinal type GC and in patients treated with adjuvant therapy. Overall, we highlighted the relevance of TRPV2 not only as a prognostic biomarker but also as a potential therapeutic target to improve GC treatment efficacy.
Validation of the Beliefs about Children’s Adjustment in Same-Sex Families Scale in Italian University Students

ABSTRACT Assessing attitudes toward same-sex parenting has important implications for both intervention and prevention programs designed to foster the tolerance and normality of sexual and family diversity. Few empirically validated measures of rejection of same-sex parenting have been developed. The current study examined the psychometric properties of an Italian translation of the Beliefs about Children’s Adjustment in Same-Sex Families Scale, a 14-item scale distributed in two subscales that measure direct and subtle rejection toward same-sex parenting, for its use in assessing heterosexual people’s attitudes toward same-sex parents in Italy. The sample consisted of 344 Italian heterosexual university students (113 men) with a mean age of 20.48 years (SD = 1.60). The results showed that the scale has a good factor structure (via confirmatory factor analysis) and satisfactory reliability and acceptable criterion-related validity. Therefore, the BCASSFS can be used to measure opposition toward gay and lesbian parenting in the Italian context.
The EDGE-CALIFA Survey: Evidence for Pervasive Extraplanar Diffuse Ionized Gas in Nearby Edge-on Galaxies

We investigate the prevalence, properties, and kinematics of extraplanar diffuse ionized gas (eDIG) in a sample of 25 edge-on galaxies selected from the CALIFA survey. We measure ionized gas scale heights from Hα and find that 90% have measurable scale heights with a median of kpc. From the Hα kinematics, we find that 60% of galaxies show a decrease in the rotation velocity as a function of height above the midplane. This lag is characteristic of eDIG, and we measure a median lag of 21 km s−1 kpc−1, which is comparable to lags measured in the literature. We also investigate variations in the lag with radius. H i lags have been reported to systematically decrease with galactocentric radius. We find both increasing and decreasing ionized gas lags with radius, as well as a large number of galaxies consistent with no radial lag variation, and investigate these results in the context of internal and external origins for the lagging ionized gas. We confirm that the [S ii]/Hα and [N ii]/Hα line ratios increase with height above the midplane as is characteristic of eDIG. The ionization of the eDIG is dominated by star-forming complexes (leaky H ii regions). We conclude that the lagging ionized gas is turbulent ejected gas likely resulting from star formation activity in the disk as opposed to gas in the stellar thick disk or bulge. This is further evidence for the eDIG being a product of stellar feedback and for the pervasiveness of this WIM-like phase in many local star-forming galaxies.
Rural Emergency Laparotomy Audit

Emergency laparoscopy/laparotomy is associated with high levels of mortality. The aim of this study was to determine whether outcomes following emergency laparoscopy/laparotomy in rural and regional South Australian hospitals were comparable to those reported in the National Emergency Laparotomy Audit and Perth Emergency Laparotomy Audit.
Relationship Between Resting Heart Rate Variability and Intermittent Endurance Performance in Novice Soccer Players

ABSTRACT Purpose: This study examined the relationships between the Yo-Yo Intermittent Recovery Test level 1 (Yo-Yo IR1) and resting heart rate variability (HRV) and submaximal 5ʹ-5ʹ test derived measures in novice male soccer players. Methods: Forty players (11.54 ± 0.58 years) from a soccer academy participated in this study, performing physical tests on two different days, separated by 48 h, as follows: (day 1) resting HRV and Yo-Yo IR1 test, and (day 2) anthropometric assessments (for peak height velocity assessment [PHV]) and the 5ʹ-5ʹ test. The Pearson product-moment correlation coefficient was used to determine the correlations between Yo-Yo IR1 performance and the remaining variables. A partial correlation analysis was further performed using age, stature, body mass, distance to PHV, and age at PHV as “confounders. ” Results: The highest correlation score was observed between Yo-Yo IR1 performance and resting HRV, when the absolute age was used as confounder (r = 0.72; p < .05). Conclusions: We observed that a practical measure of parasympathetic activity at rest is largely associated with performance obtained during a traditional intermittent endurance performance test.
Experimental Verification of Isotropic and Anisotropic Anhysteretic Magnetization Models

The anhysteretic magnetization curve is the key element of modeling magnetic hysteresis loops. Despite the fact that it is intensively exploited, known models of anhysteretic curve have not been verified experimentally. This paper presents the validation of four anhysteretic curve models considering four different materials, including isotropic, such as Mn-Zn soft ferrite, as well as anisotropic amorphous and nanocrystalline alloys. The presented results indicate that only the model that considers anisotropic energy is valid for a wide set of modern magnetic materials. The most suitable of the verified models is the anisotropic extension function-based model, which considers uniaxial anisotropy.