Miracle-dz/UniFAR-FTUs huggingface dataset:
High‐resolution DNA melt‐curve analysis for cost‐effective mass screening of pairwise species interactions

Ecological studies of pairwise interactions are constrained by the methods available for rapid species identification of the interacting organisms. The resolution of data required to characterize species interaction networks at multiple spatio‐temporal scales can be intensive, and therefore laborious and costly to collect. We explore the utility of high‐resolution DNA melt‐curve analysis (HRM) as a rapid species identification method. An approach was developed to identify organisms at the pairwise interaction level, with particular application to cryptic species interactions that are traditionally difficult to study. Here, we selected a challenging application; to identify the presence/absence of pathogenic fungi (Sporothrix inflata, Ophiostoma nigrocarpum and Ophiostoma galeiforme) transported by bark beetle vectors (Hylastes ater and Hylurgus ligniperda). The technique was able to distinguish between different species of DNA within a single, pooled sample. In test applications, HRM was effective in the mass screening and identification of pathogenic fungal species carried by many individual bark beetle vectors (n = 455 beetles screened) across large geographic scales. For two of the fungal species, there was no difference in the frequency of association with either of their vectors, but for the third fungal species there was a shift in vector–pathogen associations across locations. This technique allows rapid, mass screening and characterization of species interactions at a fraction of the time and cost of traditional methods. It is anticipated that this method can be readily applied to explore other cryptic species interactions, or other studies requiring rapid generation of large data sets and/or high‐throughput efficiency.
ACUTE CHEMICAL TOXICITY OF URANIUM

Although human experience with uranium spans more than 200 years, the LD50 for acute intake in humans has not been well established. Large acute doses of uranium can produce death from chemical toxicity in rats, guinea pigs, and other small experimental animals, with variation in sensitivity among species. However, there has never been a death attributable to uranium poisoning in humans, and humans seem to be less sensitive to both acute and chronic toxic effects of uranium than other mammalian species studied. Highly relevant data on uranium toxicity in humans are available from the experience of persons administered large doses of uranium for therapy of diabetes and from acute accidental inhalation intakes. Although the data on which to establish oral and inhalation acute LD50 for uranium in humans are sparse, they are adequate to conclude that the LD50 for oral intake of soluble uranium compounds exceeds several grams of uranium and is at least 1.0 g for inhalation intakes. For intakes of uranium compounds of lesser solubility, acute LD50 values are likely to be significantly greater. It is suggested that 5 g be provisionally considered the acute oral LD50 for uranium in humans. For inhalation intakes of soluble compounds of uranium, 1.0 g of uranium is proposed as the provisional acute inhalation LD50.
Finders Keepers: Forfeiture Laws, Policing Incentives, and Local Budgets

In order to encourage anti-drug policing, both the federal government and many state governments have enacted laws that allow police agencies to keep a substantial fraction of assets that they seize in drug arrests. By adjusting their own allocations to police budgets, however, county governments can effectively undermine these incentives, capturing the additional resources for other uses. We use a rich new data set on police seizures and county spending to explore the reactions of both local governments and police to the complex incentives generated by these laws. We find that local governments do indeed offset the seizures that police make by reducing their other allocations to policing, undermining the statutory incentive created by the laws. They are more likely to do so in times of fiscal distress. Police, in turn, respond to the real net incentives for seizures, once local offsets are taken into account, not simply the incentives set out in statute. When de facto policies allow police to keep the assets they seize, they seize more. These findings have strong implications for the effectiveness of using financial incentives to solve agency problems in the provision of public goods in a federal system: agents respond to incentives, but so do intervening governments, and the effectiveness of federal and state laws in influencing agents' behavior is limited by the ability of local governments to divert funds to other uses.
Boundaryless careers and institutional resources

The ‘boundaryless career’ model has been dominant in the careers and management literatures for over a decade. It has provided valuable conceptual tools for researchers as well as a supportive ideology for those embarking on careers outside the limits of a single organization. It has stimulated a great deal of research in a variety of countries. But, along with a growing number of critics, we suggest that this model has an excessively individualistic bias which views career success as largely a function of individual proactive traits and the person-cantered social networks built by individual action. As a result it has neglected the institutional resources needed to support such careers. In this paper we outline seven different sets of needs for successful boundaryless careers: career counselling, socioemotional support, job performance and entrepreneurial skills, skill transferability and certification, labour market assistance, financial and material resources, and collective voice. We then describe institutions that currently provide at least some of the resources to meet these needs and have the potential to provide more: employers, occupational communities and associations, labour unions, employment and recruiting agencies, temporary worker agencies, community organizations, the Internet, and government at all levels. Employers can play a key role in supporting boundaryless careers by serving as points of reference for independent and contract workers, in particular those who have been their full-time employees. Rather than boundaryless, we suggest that more appropriate metaphors for the new career might be boundary crossing and boundary converging. We call for more research on the role of institutionally provided resources in supporting extra-organizational careers.
In vitro and in vivo evaluation of controlled-release matrix tablets of highly water-soluble drug applying different mw polyethylene oxides (PEO) as retardants

Abstract The aim of the work presented is to prepare a controlled-release hydrophilic matrix tablet (CMT) controlling release of highly water-soluble drug applying pure combination of high- and low-Mw PEO as matrix materials, to avoid the lag time of drug release, and to overcome incomplete release in later stages. The influences of types and amounts of different Mw PEOs used, drug loading, pH of release medium and agitation rate on drug release were evaluated. The study of uptake and erosion of matrix was conducted and mechanism of improving drug release was discussed. In vivo pharmacokinetics of the CMT and reference preparation self-made controlled-release osmotic pump tablets (COPT) were performed in beagle dogs. The optimized formulation containing 43% PEO WSR 303 and 32% PEO N750 showed a zero order release from 1 h to 12 h. In vivo results demonstrated that the CMT had similar AUC0-48 h and Cmax with the COPT but smaller Tmax than the COPT and provided a more stable therapeutic concentration compared to the COPT. In conclusion, hydrophilic matrix tablet combining only different Mw PEOs as matrix materials had very good potential to be developed into a controlled-release drug delivery system for highly water-soluble drug. Besides, its manufacturing processes were succinct which would be preferable for modern medicine industry.
Governing education through data in England: from regulation to self‐evaluation

This paper explores the relationship between changing forms of the governance of education and the growth and uses of data in the context of England – a context that can be described as the most ‘advanced’ in Europe in terms of data production and use. The paper links the shifting relations between the central department of education (variously known between the 1980s and the time of writing as the Department of Education and Science [DES], Department for Education and Skills [DfES] and the Department for Children, Schools and Families [DCSF]), the local education authorities and the schools to the growth and development of data‐based systems of inspection and performance management, and suggests that the massive growth of data has unbalanced the relations of governing and created highly centralised system steering. Recent attempts to ‘rebalance’ steering through ‘intelligent accountability’ invoke network principles and self‐regulation through self‐evaluation, and thus give the appearance of deregulation, but the centre maintains control through its management and use of data, and local government remains peripheral.
Dideoxynucleoside resistance emerges with prolonged zidovudine monotherapy. The RV43 Study Group

Human immunodeficiency virus type 1 (HIV-1) isolates resistant to zidovudine (ZDV) have previously been demonstrated to exhibit in vitro cross-resistance to other similar dideoxynucleoside agents which contain a 3'-azido group. However, cross-resistance to didanosine (ddI) or dideoxycytidine (ddC) has been less well documented. ZDV, ddI, and ddC susceptibility data have been collected from clinical HIV-1 isolates obtained by five clinical centers and their respective retrovirology laboratories. All subjects were treated only with ZDV. Clinical HIV-1 isolates were isolated, amplified, and assayed for drug susceptibility in standardized cultures of phytohemagglutinin-stimulated donor peripheral blood mononuclear cells obtained from healthy seronegative donors. All five cohorts showed a correlation between decreased in vitro susceptibility to ZDV and decreased susceptibility to ddI and ddC. For each 10-fold decrease in ZDV susceptibility, an average corresponding decrease of 2.2-fold in ddI susceptibility was observed (129 isolates studied; P < 0.001, Fisher's test of combined significance). Similarly, susceptibility to ddC decreased 2.0-fold for each 10-fold decrease in ZDV susceptibility (82 isolates studied; P < 0.001, Fisher's test of combined significance). These data indicate that a correlation exists between HIV-1 susceptibilities to ZDV and ddI or ddC for clinical HIV-1 isolates.
Self-collected breath sampling for monitoring low-level benzene exposures among automobile mechanics.

Automobile mechanics are exposed to benzene through their contact with gasoline vapor and engine exhaust. This study investigated the benzene uptake associated with these exposures. We first evaluated the reliability of self-collected breath samples among a subset of subjects and found good agreement between these samples and those collected under expert supervision (intraclass correlation coefficient 0.79, n = 69). We then used self-monitoring together with a longitudinal sampling design (with up to three measurements per worker) to measure benzene in air and benzene in end-exhaled breath among 81 workers from 12 automobile repair garages in North Carolina. A statistically significant difference (P < 0.0001, Mann-Whitney rank sum test) was observed between non-smokers and smokers for post-exposure benzene concentration in breath (median values of 18.9 and 39.1 micro g/m(3), respectively). Comparing pre- and post-exposure breath concentrations within these two groups, the difference was significant among non-smokers (P < 0.0001) but not significant among smokers (P > 0.05). Mixed effects regression analysis using backwards elimination yielded five significant predictors of benzene concentration in breath, namely benzene exposure (P < 0.0001), pre-exposure benzene concentration in breath (P = 0.021), smoking status (P < 0.0001), fuel system work (P = 0.0043) and carburetor cleaner use (P < 0.0001). The between-person variance component comprised only 28% of the total variance in benzene levels in breath, indicating that differences among individuals related to physiological and metabolic characteristics had little influence on benzene uptake among these workers.
Strategies of Legitimacy Through Social Media: The Networked Strategy

How can corporations develop legitimacy when coping with stakeholders who have multiple, often conflicting sustainable development (SD) agendas? We address this question by conducting an in‐depth longitudinal case study of a corporation's stakeholder engagement in social media and propose the concept of a networked legitimacy strategy. With this strategy, legitimacy is gained through participation in non‐hierarchical open platforms and the co‐construction of agendas. We explore the organizational transition needed to yield this new legitimacy approach. We argue that, in this context, legitimacy gains may increase when firms are able to reduce the control over the engagements and relate non‐hierarchically with their publics. We contribute to the extant literature on political corporate social responsibility and legitimacy by providing an understanding of a new context for engagement that reconfigures cultural, network, and power relations between the firm and their stakeholders in ways that challenge previous forms of legitimation.
Technical efficiency in African agriculture: Is it catching up or lagging behind?

This article uses recent advances in data envelopment analysis, bootstrap data envelopment analysis, to investigate whether technical efficiency in the agricultural sector of 33 African countries improved (catching up) for the period 1966–2001. We also investigate whether there is evidence of efficiency catching‐up within the five regions of Central, Eastern, Western, Northern and Southern Africa. Overall, the results show no evidence for efficiency catching‐up in the entire sample. However, efficiency differed across countries and regions with evidence of catching‐up within the East African countries. Our analyses point to the need for policies that improve technological uptake in African agriculture. Copyright © 2013 John Wiley & Sons, Ltd.
Hidden keys to survival: the type, density, pattern and functional role of emperor penguin body feathers

Antarctic penguins survive some of the harshest conditions on the planet. Emperor penguins breed on the sea ice where temperatures drop below −40°C and forage in −1.8°C waters. Their ability to maintain 38°C body temperature in these conditions is due in large part to their feathered coat. Penguins have been reported to have the highest contour feather density of any bird, and both filoplumes and plumules (downy feathers) are reported absent in penguins. In studies modelling the heat transfer properties and the potential biomimetic applications of penguin plumage design, the insulative properties of penguin plumage have been attributed to the single afterfeather attached to contour feathers. This attribution of the afterfeather as the sole insulation component has been repeated in subsequent studies. Our results demonstrate the presence of both plumules and filoplumes in the penguin body plumage. The downy plumules are four times denser than afterfeathers and play a key, previously overlooked role in penguin survival. Our study also does not support the report that emperor penguins have the highest contour feather density.
Partial lipodystrophy in patients who have undergone hematopoietic stem cell transplantation during childhood: an institutional cross-sectional survey

Abstract. Partial lipodystrophy (PD), a condition similar to metabolic syndrome without obesity, is one of the late complications of hematopoietic stem cell transplantation (HSCT) performed during childhood. We aimed to investigate the prevalence and risk factors of PD. A cross-sectional survey was performed in a children’s hospital, targeting patients treated for a malignancy or hematological disorder, and who were disease-free for > 24 mo. PD was defined as gluteal lipoatrophy and lipohypertrophy of the cheeks or neck associated with diabetes and/or fatty liver disease. In total, 65 patients were enrolled. Six patients (9.2%) were judged to have PD, all of whom had received 10–14 Gy total body irradiation. Compared with the patients without PD, patients with PD were older at investigation (P < 0.01), had a longer elapsed time following HSCT (P < 0.01), had more frequent disease recurrence (P < 0.05), and were more likely to have undergone multiple HSCT (P < 0.05). In addition, they had higher blood pressure and showed higher levels of low-density lipoprotein-cholesterol and triglycerides, whereas their adiponectin levels were significantly lower. In conclusion, a large number of patients developed PD following HSCT, with unfavorable metabolic profiles at a later age, especially when they experienced a complex disease course.
Theory and Misbehavior of First-Price Auctions: Reply

Economic theory has been under severe attack in recent years. The source of this attack has been the observation of apparently robust behavioral anomalies in decisions that experimental subjects make in controlled environments. The implication of these observations drawn by some is that many of the fundamental tenets of economic theory are systematically misleading as a descriptive model of human behavior. Many alternative models of individual and group behavior have been proposed which can account for some or all of the apparent anomalies. In the "Theory and Misbehavior of FirstPrice Auctions" (Harrison, 1989), I argued that the effort to extend or generalize received auction theory as a response to such anomalies was misdirected. Specifically, I argued that the anomalies observed in the experiments in question may simply reflect the failure of the experiment to meet widely accepted sufficient conditions for a valid controlled experiment proposed by Vernon Smith (1982 pp. 930-9). The result of this failure is simply that the opportunity cost of "misbehavior" in these experiments is, by any reasonable standard, minuscule. Observed "anomalies" may then not be anomalies at all: they reflect theoretically consistent behavior under conditions where misbehavior is virtually costless. My critique is quite general in going well beyond auction theory and sealed-bid experiments. Perhaps for this reason there is some truth in the assessment of John D. Hey (1991 p. 195) that it "has stirred the passions of the experimental community throughout America. " The sad corollary of that assessment, however, is that experimentalists must be a pretty dull lot if such modest concerns as mine stir their passions. Section I restates the payoff-dominance critique in general terms to introduce the nonexperimentalist to the main issues. To address some of the issues raised by my critics, Section II contains a detailed numerical example of an important experimental procedure for eliciting the certainty-equivalent of any lottery that uses the G. M. Becker et al. (1964) procedure. In Section III, the generality of the problem is briefly catalogued, so as to emphasize that this is a debate over much broader methodological matters than sealed-bid auction experiments. Section IV addresses directly some of the specific comments of my critics. Section V identifies a number of qualifications to my critique of existing experimental practice.
Equity Value, Implied Cost of Equity and Shareholders’ Real Options

This study investigates the effects of shareholders’ real options on (i) firm financial performance and (ii) estimations of the implied cost of equity. After measuring the equity value of steady-state operations using the residual income model, and the abandonment and expansion options using the Black-Scholes option pricing model, I find that firms with a large expansion (abandonment) option value experience better (worse) financial performance than those with a small such value. I also find that ignoring the expansion (abandonment) option results in a downward bias in implied cost of equity estimates by an average of 1.23 percentage points.
Exploration of the Impact of Gambling and Problem Gambling on Pacific Families and Communities in New Zealand

Background Pacific people have consistently been at higher risk than other ethnicities for developing problem gambling. Despite this higher risk, there is very little research on Pacific people’s gambling and the impacts of problem gambling. There is even less gambling research focusing on different Pacific ethnicities, with small Samoan and Tongan studies being the only research to date. Most Pacific research is conducted with participants described as ‘Pacific’ irrespective of their ethnicity.
Issue Unbundling Via Citizens' Initiatives

The role of citizens' intitiatives figures prominantly in contemporary debates on constitutional change. A basic question is why are initiatives necessary in a representative democracy where candidates must already compete for the right to control policy? This Paper offers one answer to this question. In a representative democracy, the bundling of issues, together with the fact that citizens have only one vote, means that policy outcomes on specific issues may diverge far from what the majority of citizens want. In such circumstances, allowing citizens to put legislation directly on the ballot permits the 'unbundling' of these issues, which forces a closer relationship between policy outcomes and popular preferences. To the extent that it is condsidered socially undesirable for outcomes on specific issues to stray too far from what the majority wants, this creates a role for citizens initiatives.
The Influence of Competitive Intensity and Market Dynamism on Knowledge Management Capabilities of Multinational Corporation Subsidiaries

The authors use the environment–strategy–performance theoretical framework to examine the direct influence of the environmental market conditions of competitive intensity and market dynamism on knowledge management capabilities of multinational corporation (MNC) subsidiaries and resultant subsidiary performance. The results of a survey of the managers of Croatian subsidiaries of foreign MNCs indicate that though both competitive intensity and market dynamism individually influence knowledge management capabilities, when examined jointly, market dynamism is a more influential environmental market condition than competitive intensity. The authors also find a significant, positive relationship between a subsidiary's knowledge management capabilities and its performance. The findings indicate the importance of understanding local market conditions and developing proper strategic configurations for MNC subsidiaries.