Miracle-dz/UniFAR-FTUs huggingface dataset:
Evidence for a direct, nucleotide-sensitive interaction between actin and liver cell membranes

We have investigated the association of actin with membranes isolated from rat liver. A plasma membrane-enriched fraction prepared by homogenization in a low salt/CaCl2 buffer was found to contain a substantial amount of residual actin which could be removed by treatment with 1 M Na2CO3/NaHCO3, pH 10.5. Using a sedimentation binding assay that uses gelsolin to shorten actin filaments and render membrane binding saturable (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol. 102:2067-2075), we found that membranes stripped of endogenous actin bound 125I-actin in a specific and saturable manner. Scatchard plots of binding data were linear, indicating a single class of binding sites with a Kd of 1.6 microns; 66 micrograms actin bound/mg membrane protein at saturation. Binding of actin to liver cell membranes was negligible with unstripped membranes, was competed by excess unlabeled actin, and was greatly reduced by preheating or proteolytic digestion of the membranes. Kinetic measurements showed that binding had an initial lag phase and was strongly temperature dependent. The binding of actin to liver cell membranes was also found to be competitively inhibited by ATP and other nucleotides, including the nonhydrolyzable analogue AMP-PNP. We conclude that we have reconstituted an interaction between actin and integral membrane proteins from the rat liver. This interaction exhibits a number of distinctive features which have not been observed in other actin- membrane systems.
Occurrence of Six Honeybee Viruses in Diseased Austrian Apiaries

ABSTRACT The occurrence, prevalence, and distribution patterns of acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbrood virus (SBV) were investigated in 90 Austrian honeybee colonies suffering from symptoms of depopulation, sudden collapse, paralysis, or dark coloring by employing reverse transcription-PCR. Infestation with parasites was also recorded. The samples originated from all parts of Austria. The most prevalent virus was DWV, present in 91% of samples, followed by ABPV, SBV, and BQCV (68%, 49%, and 30%, respectively). CBPV was detected in 10% of colonies, while KBV was not present in any sample. In most samples, more than one virus was identified. The distribution pattern of ABPV, BQCV, CBPV, and SBV varied considerably in the different geographic regions investigated, while DWV was widespread in all Austrian federal states. In bees that showed dark coloring and disorientation, CBPV was always detected. Simultaneous infections of DWV and ABPV were most frequently observed in colonies suffering from weakness, depopulation, and sudden collapse. Bees obtained from apparently healthy colonies within the same apiaries showed a similar distribution pattern of viruses; however, the relative virus load was 10 to 126 times lower than in bees from diseased colonies. A limited number of bee samples from surrounding central European countries (Germany, Poland, Hungary, and Slovenia) were also tested for the presence of the above viruses. Variances were found in the distribution of BQCV and SBV.
Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves' Orbitopathy: Clinical Implications

Purpose. To assess FGF-β, TGF-β, and COX2 expression and immunocompetent cells in the orbital tissue of patients with severe and mild Graves' orbitopathy. Patients and Methods. Orbital tissue was taken from 27 patients with GO: (1) severe GO (n = 18), the mean clinical activity score (CAS) being 8.5 (SD 2.5); and (2) mild GO (n = 9), the mean CAS being 2.2 (SD 0.8), and from 10 individuals undergoing blepharoplasty. The expression of CD4+, CD8+, CD20+, and CD68 and FGF-β, TGF-β, and COX2 in the orbital tissue was evaluated by immunohistochemical methods. Results. We demonstrated predominant CD4+ T cells in severe GO. CD68 expression was observed in the fibrous connective area of mild GO and was robust in severe GO, while the prominent TGF-β expression was seen in all GO. Increased FGF-β expression was observed in the fibroblasts and adipocytes of severe GO. No expression of COX2 was found in patients with GO. Conclusions. Macrophages and CD4 T lymphocytes are both engaged in the active/severe and long stage of inflammation in the orbital tissue. FGF-β and TGF-β expression may contribute to tissue remodeling, fibrosis, and perpetuation of inflammation in the orbital tissue of GO especially in severe GO.
Emerging links between homeostatic synaptic plasticity and neurological disease

Homeostatic signaling systems are ubiquitous forms of biological regulation, having been studied for hundreds of years in the context of diverse physiological processes including body temperature and osmotic balance. However, only recently has this concept been brought to the study of excitatory and inhibitory electrical activity that the nervous system uses to establish and maintain stable communication. Synapses are a primary target of neuronal regulation with a variety of studies over the past 15 years demonstrating that these cellular junctions are under bidirectional homeostatic control. Recent work from an array of diverse systems and approaches has revealed exciting new links between homeostatic synaptic plasticity and a variety of seemingly disparate neurological and psychiatric diseases. These include autism spectrum disorders, intellectual disabilities, schizophrenia, and Fragile X Syndrome. Although the molecular mechanisms through which defective homeostatic signaling may lead to disease pathogenesis remain unclear, rapid progress is likely to be made in the coming years using a powerful combination of genetic, imaging, electrophysiological, and next generation sequencing approaches. Importantly, understanding homeostatic synaptic plasticity at a cellular and molecular level may lead to developments in new therapeutic innovations to treat these diseases. In this review we will examine recent studies that demonstrate homeostatic control of postsynaptic protein translation, retrograde signaling, and presynaptic function that may contribute to the etiology of complex neurological and psychiatric diseases.
Touching moments: desire modulates the neural anticipation of active romantic caress

A romantic caress is a basic expression of affiliative behavior and a primary reinforcer. Given its inherent affective valence, its performance also would imply the prediction of reward values. For example, touching a person for whom one has strong passionate feelings likely is motivated by a strong desire for physical contact and associated with the anticipation of hedonic experiences. The present study aims at investigating how the anticipatory neural processes of active romantic caress are modulated by the intensity of the desire for affective contact as reflected by passionate feelings for the other. Functional magnetic resonance imaging scanning was performed in romantically involved partners using a paradigm that allowed to isolate the specific anticipatory representations of active romantic caress, compared with control caress, while testing for the relationship between neural activity and measures of feelings of passionate love for the other. The results demonstrated that right posterior insula activity in anticipation of romantic caress significantly co-varied with the intensity of desire for union with the other. This effect was independent of the sensory-affective properties of the performed touch, like its pleasantness. Furthermore, functional connectivity analysis showed that the same posterior insula cluster interacted with brain regions related to sensory-motor functions as well as to the processing and anticipation of reward. The findings provide insight on the neural substrate mediating between the desire for and the performance of romantic caress. In particular, we propose that anticipatory activity patterns in posterior insula may modulate subsequent sensory-affective processing of skin-to-skin contact.
Tumor Mesenchymal Stem-Like Cell as a Prognostic Marker in Primary Glioblastoma

The isolation from brain tumors of tumor mesenchymal stem-like cells (tMSLCs) suggests that these cells play a role in creating a microenvironment for tumor initiation and progression. The clinical characteristics of patients with primary glioblastoma (pGBM) positive for tMSLCs have not been determined. This study analyzed samples from 82 patients with pGBM who had undergone tumor removal, pathological diagnosis, and isolation of tMSLC from April 2009 to October 2014. Survival, extent of resection, molecular markers, and tMSLC culture results were statistically evaluated. Median overall survival was 18.6 months, 15.0 months in tMSLC-positive patients and 29.5 months in tMSLC-negative patients (P = 0.014). Multivariate cox regression model showed isolation of tMSLC (OR = 2.5, 95% CI = 1.1~5.6, P = 0.021) showed poor outcome while larger extent of resection (OR = 0.5, 95% CI = 0.2~0.8, P = 0.011) has association with better outcome. The presence of tMSLCs isolated from the specimen of pGBM is associated with the survival of patient.
Antioxidant metabolism during blood storage and its relationship to posttransfusion red cell survival

The status of the erythrocyte antioxidant defense system and its relationship to posttransfusion red cell survival were determined in erythrocytes stored for 35 or 42 days in CPD‐A1 anticoagulant with a saline‐adenine‐glucose additive. As storage progressed, there was a significant increase in incubated Heinz body formation (P < .001) and a significant decrease in reduced glutathione (GSH) stability (P < .001). Stimulated pentose phosphate shunt activity also declined during storage (P < .06), while unstimulated shunt activity remained unchanged. The increase in Heinz body formation was associated with decreased GSH stability (r = –.77, P < .001), which in turn was associated with the decline in stimulated pentose shunt activity (r = .67, P < .001). The changes in Heinz body formation (r = ‐.85), GSH stability (r = .83). and stimulated pentose shunt activity (r = .54) were all significantly (P < .001) related to the decline in adenosine triphosphate (ATP) content of the erythrocyte. Red cell survival 24 hours after transfusion was significantly related to the GSH stability (r = .80, P < .001) and to the ATP concentration (r = .76, P < .005) on the day of transfusion. Thus, dysfunction of the erythrocyte antioxidant defense system occurs during blood storage and appears to be related, in part, to ATP depletion. The ability to maintain a normal reduced glutathione concentration during oxidant stress appears to be an important determinant of red cell survival in the peritransfusion period.
Active Brownian motion tunable by light

Active Brownian particles are capable of taking up energy from their environment and converting it into directed motion; examples range from chemotactic cells and bacteria to artificial micro-swimmers. We have recently demonstrated that Janus particles, i.e. gold-capped colloidal spheres, suspended in a critical binary liquid mixture perform active Brownian motion when illuminated by light. In this paper, we investigate in more detail their swimming mechanism, leading to active Brownian motion. We show that the illumination-borne heating induces a local asymmetric demixing of the binary mixture, generating a spatial chemical concentration gradient which is responsible for the particle’s self-diffusiophoretic motion. We study this effect as a function of the functionalization of the gold cap, the particle size and the illumination intensity: the functionalization determines what component of the binary mixture is preferentially adsorbed at the cap and the swimming direction (towards or away from the cap); the particle size determines the rotational diffusion and, therefore, the random reorientation of the particle; and the intensity tunes the strength of the heating and, therefore, of the motion. Finally, we harness this dependence of the swimming strength on the illumination intensity to investigate the behavior of a micro-swimmer in a spatial light gradient, where its swimming properties are space-dependent.
Vascular flow reserve as a link between long‐term blood pressure level and physical performance capacity in mammals

Mean arterial pressure (MAP) is surprisingly similar across different species of mammals, and it is, in general, not known which factors determine the arterial pressure level. Mammals often have a pronounced capacity for sustained physical performance. This capacity depends on the vasculature having a flow reserve that comes into play as tissue metabolism increases. We hypothesize that microvascular properties allowing for a large vascular flow reserve is linked to the level of the arterial pressure.To study the interaction between network properties and network inlet pressure, we developed a generic and parsimonious computational model of a bifurcating microvascular network where diameter and growth of each vessel evolves in response to changes in biomechanical stresses. During a simulation, the network develops well‐defined arterial and venous vessel characteristics. A change in endothelial function producing a high precapillary resistance and thus a high vascular flow reserve is associated with an increase in network inlet pressure. Assuming that network properties are independent of body mass, and that inlet pressure of the microvascular network is a proxy for arterial pressure, the study provides a conceptual explanation of why high performing animals tend to have a high MAP.
Vygotsky and education: Cognitive development and formal schooling: The evidence from cross-cultural research

It is the dilemma of psychology to deal as a natural science with an object that creates history. Ernst Boesch My goal in this chapter is to summarize the implications for understanding cognitive development of the past three decades of intensive cross-cultural research on the cognitive consequences of formal educational experience during middle childhood. Furthermore, in concert with the theme of this book, I will approach this topic from the perspective of the sociohistorical school associated with the names of Lev S. Vygotsky, Alexei N. Leontiev, Alexander R. Luria, and their followers. Some basic presuppositions As described by Luria (1979), the sociohistorical school of psychology emerged in the 1920s as an explicit attempt to resolve the widely perceived dilemma commented on by Ernst Boesch in the epigraph with which this chapter begins: Scientific psychology, conceived as an experimental discipline, depends upon the assumption that the processes it studies are universal (and hence not dependent upon the exigencies of time and place), yet human beings manifestly are creatures who create history, rendering psychological processes historically contingent (and thus inappropriate objects of study using experimental methods). Wilhelm Wundt, titular father of scientific psychology, was acutely aware of this problem. His solution was to declare that psychology must be two sciences. The first, experimental psychology, was to be an experimental, explanatory science, in the mold of the natural sciences such as physics. Its task was to be the analysis of the contents of individual human consciousness into its elements and a specification of the universal laws according to which these elements combine.
Localization of dopamine D1 and D2 receptors in the rat neostriatum: Synaptic interaction with glutamate‐ and GABA‐containing axonal terminals

In order to determine the synaptic interactions between the glutamate‐ and GABA‐containing axonal terminals and the two subpopulations of medium spiny neurons in the rat neostriatum, double immunocytochemistry was performed. Sections of perfuse‐fixed rats were used. Immunoreactivity for dopamine D1 and D2 receptors was used as a marker for the two subpopulations of spiny neurons that give rise to the direct and indirect pathways, respectively. Receptor immunoreactivity was first revealed by preembedding immunostaining. Postembedding colloidal gold labeling was then performed to reveal immunoreactivity for glutamate or GABA. The results were analyzed at the electron microscopic level. Both the D1‐immunoreactive, presumed striatonigral/entopeduncular neurons, and the D2‐immunoreactive, presumed striatopallidal neurons, were found to receive qualitatively similar synaptic inputs from glutamate‐immunoreactive terminals and GABA‐immunoreactive terminals. The present results indicate that the different classes of spiny neurons are thus likely to be under a similar regime of excitatory and inhibitory control. Synapse 38:413–420, 2000. © 2000 Wiley‐Liss, Inc.
Achieving Target Equilibria in Network Routing Games without Knowing the Latency Functions

The analysis of network routing games typically assumes, right at the onset, precise and detailed information about the latency functions. Such information may, however, be unavailable or difficult to obtain. Moreover, one is often primarily interested in enforcing a desirable target flow as the equilibrium by suitably influencing player behavior in the routing game. We ask whether one can achieve target flows as equilibria without knowing the underlying latency functions. Our main result gives a crisp positive answer to this question. We show that, under fairly general settings, one can efficiently compute edge tolls that induce a given target multicommodity flow in a nonatomic routing game using a polynomial number of queries to an oracle that takes candidate tolls as input and returns the resulting equilibrium flow. This result is obtained via a novel application of the ellipsoid method, and applies to arbitrary multicommodity settings and non-linear latency functions. Our algorithm extends easily to many other settings, such as (i) when certain edges cannot be tolled or there is an upper bound on the total toll paid by a user, and (ii) general nonatomic congestion games. We obtain tighter bounds on the query complexity for series-parallel networks, and single-commodity routing games with linear latency functions, and complement these with a query-complexity lower bound applicable even to single-commodity routing games on parallel-link graphs with linear latency functions. We also explore the use of Stackelberg routing to achieve target equilibria and obtain strong positive results for series-parallel graphs. Our results build upon various new techniques that we develop pertaining to the computation of, and connections between, different notions of approximate equilibrium, properties of multicommodity flows and tolls in series-parallel graphs, and sensitivity of equilibrium flow with respect to tolls. Our results demonstrate that one can indeed circumvent the potentially-onerous task of modeling latency functions, and yet obtain meaningful results for the underlying routing game.
Geodesic Finite Mixture Models

We present a novel approach for learning a finite mixture model on a Riemannian manifold in which Euclidean metrics are not applicable and one needs to resort to geodesic distances consistent with the manifold geometry. For this purpose, we draw inspiration on a variant of the expectation-maximization algorithm, that uses a minimum message length criterion to automatically estimate the optimal number of components from multivariate data lying on an Euclidean space. In order to use this approach on Riemannian manifolds, we propose a formulation in which each component is defined on a different tangent space, thus avoiding the problems associated with the loss of accuracy produced when linearizing the manifold with a single tangent space. Our approach can be applied to any type of manifold for which it is possible to estimate its tangent space. In particular, we show results on synthetic examples of a sphere and a quadric surface and on a large and complex dataset of human poses, where the proposed model is used as a regression tool for hypothesizing the geometry of occluded parts of the body.
Human language identification with reduced spectral information

We conducted human language identification (LID) experiments using signals with reduced segmental information in pursuit of cues that humans use in their remarkable LID ability, which may be applicable to the development of robust automatic LID. American English and Japanese excerpts from the OGI-TS were processed by (1) spectral-envelope removal (SER) and (2) temporal-envelope modulation. With the SER signal, where the spectral-envelope is eliminated, humans could still identify the languages fairly successfully (85.2%). With the TEM signal, composed of white-noise driven, combined intensity envelopes from several frequency bands, the identification rate rose from 62.5% to 93.8% corresponding to the increasing number of bands from 1 to 4. These results, though with a limited number of languages, indicate that humans can identify languages using signal with its segmental information much reduced — in acoustic terms much reduced in spectral information.
Behavioral and Brain Functions

Background: Working memory performance is important for maintaining functioning in cognitive, academic and social activities. Previous research suggests there are prevalent working memory deficits in children with attention deficit hyperactivity disorder (ADHD). There is now a growing body of literature characterizing working memory functioning according to ADHD subtypes in children. The expression of working memory deficits in adults with ADHD and how they vary according to subtype, however, remains to be more fully documented. Methods: This study assessed differences in working memory functioning between Normal Control (NC) adults (N = 18); patients with ADHD, Combined (ADHD-CT) Type ADHD (N = 17); and ADHD, Inattentive (ADHD-IA) Type (N = 16) using subtests from the Wechsler Adult Intelligence Scale-III and Wechsler Memory Scale-III and the Paced Auditory Serial Addition Task (PASAT). Results: The ADHD groups displayed significant weaknesses in contrast to the NC group on working memory tests requiring rapid processing and active stimulus manipulation. This included the Letter-Number-Sequencing test of the Wechsler scales, PASAT omission errors and the longest sequence of consecutive correct answers on the PASAT. No overall ADHD group subtype differences emerged; however differences between the ADHD groups and the NC group varied depending on the measure and the gender of the participants. Gender differences in performance were evident on some measures of working memory, regardless of group, with males performing better than females. Conclusion: In general, the data support a dimensional interpretation of working memory deficits experienced by the ADHD-CT and ADHD-IA subtypes, rather than an absolute difference between subtypes. Future studies should test the effects of processing speed and load on subtype performance and how those variables interact with gender in adults with ADHD. Background Attention deficit hyperactivity disorder (ADHD) may be the most common childhood DSM-IV diagnosis [1], with 30% to 50% of cases persisting into adulthood [2]. Published: 15 December 2006 Behavioral and Brain Functions 2006, 2:43 doi:10.1186/1744-9081-2-43 Received: 21 March 2006 Accepted: 15 December 2006 This article is available from: http://www.behavioralandbrainfunctions. com/content/2/1/43 © 2006 Schweitzer et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.