Miracle-dz/UniFAR-FTUs huggingface dataset:
Collagen fibrillogenesis in situ: Fibril segments undergo post‐depositional modifications resulting in linear and lateral growth during matrix development

Elucidating how collagen fibril growth is regulated is important in determining how tissues are assembled. Fibrils are deposited as segments. The growth of these segments is an important determinant of tissue architecture, stability, and mechanical attributes. Fibril segments were isolated from developing tendons and their structure characterized. The post‐depositional changes leading to linear and lateral growth of fibrils also were examined. Segments extracted from 14‐day chicken embryo tendons had a mean length of 29 μm. The segments were asymmetric, having a short and a long tapered end. Most of the segments were centrosymmetric with respect to molecular packing. Segments extracted from 12‐to 16‐day tendons had the same structure, but mean segment length increased incrementally due to the addition of an increasingly large population of longer segments. At 17 days of development there was a precipitous increase in segment length. The morphological data indicate that the increase in length was the result of lateral associations among adjacent segments. Analysis demonstrated that this fibril growth was associated with a significant decrease in fibril associated decorin. Using immunoelectron microscopy, decorin was seen to decrease significantly at 18 days of development. When decorin content was biochemically determined, a decrease also was observed. Decorin mRNA also decreased relative to fibrillar collagen mRNA during the same period. These data support the hypothesis that a decrease in fibril‐associated decorin is necessary for fibril growth associated with tissue maturation. Growth through post‐depositional fusion allows for appositional and intercalary growth and would be essential for normal development, growth, and repair. © 1995 Wiley‐Liss, Inc.
The Three Horsemen of Growth: Plague, War and Urbanization in Early Modern Europe

How did Europe overtake China? We construct a simple Malthusian model with two sectors, and use it to explain how European per capita incomes and urbanization rates surged ahead of Chinese ones. Productivity growth can only explain a small fraction of rising living standards. Population dynamics - changes of the birth and death schedules - were far more important drivers of the long-run Malthusian equilibrium. The Black Death raised wages substantially, creating important knock-on effects. Because of Engel’s Law, demand for urban products increased, raising urban wages and attracting migrants from rural areas. European cities were unhealthy, especially compared to Far Eastern ones. Urbanization pushed up aggregate death rates. This effect was reinforced by more frequent wars (fed by city wealth) and disease spread by trade. Thus, higher wages themselves reduced population pressure. We show in a calibration exercise that our model can account for the sharp rise in European urbanization as well as permanently higher per capita incomes in 1700, without technological change. Wars contributed importantly to the rise of Europe, even if they had negative short-run effects. We also examine intra-European growth, using a panel of European states in the period 1300-1700. Estimation results suggest that war frequency can explain a good share of divergent fortunes within Europe as well.
Application of Natural Language Processing to Determine User Satisfaction in Public Services

Research on customer satisfaction has increased substantially in recent years. However, the relative importance and relationships between different determinants of satisfaction remains uncertain. Moreover, quantitative studies to date tend to test for significance of pre-determined factors thought to have an influence with no scalable means to identify other causes of user satisfaction. The gaps in knowledge make it difficult to use available knowledge on user preference for public service improvement. Meanwhile, digital technology development has enabled new methods to collect user feedback, for example through online forums where users can comment freely on their experience. New tools are needed to analyze large volumes of such feedback. Use of topic models is proposed as a feasible solution to aggregate open-ended user opinions that can be easily deployed in the public sector. Generated insights can contribute to a more inclusive decision-making process in public service provision. This novel methodological approach is applied to a case of service reviews of publicly-funded primary care practices in England. Findings from the analysis of 145,000 reviews covering almost 7,700 primary care centers indicate that the quality of interactions with staff and bureaucratic exigencies are the key issues driving user satisfaction across England.
Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

the present study was undertaken to detect the incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and the clonal relationship of the isolates in healthy kuroiler birds. a total of 80 cloacal swabs from kuroilers were collected randomly from West Bengal, India. the use of costly antimicrobials (cephalosporins) was not practiced by farmers. Escherichia coli was isolated and identified by standard biochemical tests and 16SrRNA-PCR. All the E. coli isolates, including controls, were subjected to PCr for detection of blaCTX-M, blaTEM, blaSHV, and blaCMY-2 genes. By comparing the raPD-banding pattern, the phylogenetic relationship among the isolates was established. all the isolates were tested for phenotypical resistance against other antibiotics. In total, 60 isolates were identified as E. coli from the kuroilers studied (n = 80). among them, 12 (20%) isolates possessed one of the studied extended-spectrum cephalosporinase genes. among the studied genes, blaTEM and blaSHV were detected in 6 (10%) and 12 (20%) E. coli isolates, respectively. None of the E. coli isolates possessed blaCTX-M and blaCMY-2. In phylogenetic analysis, the strains isolated from same localities with similar genetic profile were grouped into the same cluster. resistance of extended-spectrum cephalosporinase gene possessing E. coli isolates was observed most frequently against ampicillin/cloxacillin, co-trimoxazole, amoxyclav, piperacillin, ceftriaxone, and tetracycline. Kuroiler birds with no cephalosporin usage profile may act as a reservoir of extended-spectrum cephalosporinase gene possessing E. coli. This is the first systematic study in kuroilers, to raise the awareness of consumers regarding the possibility of transmission of antimicrobial resistant E. coli from them.
The Chameleon-like Nature of Evaluative Adjectives

This is is an experimental study of the semantics of the construction NP was (not) Adj to VP where Adj is an evaluative adjective such as stupid. We show that in the simple past tense this construction is predominantly factive for most people but implicative for some. We also demonstrate that the interpretations are sensitive to preconceptions about how suitable the adjective is as a characterization of the event described by the innitival clause. This Consonance/Dissonance eect gives the construction its chameleon-like characteristics.
Ameliorative Effects of a Combination of Baicalin, Jasminoidin and Cholic Acid on Ibotenic Acid-Induced Dementia Model in Rats

Aims To investigate the therapeutic effects and acting mechanism of a combination of Chinese herb active components, i.e., a combination of baicalin, jasminoidin and cholic acid (CBJC) on Alzheimer’s disease (AD). Methods Male rats were intracerebroventricularly injected with ibotenic acid (IBO), and CBJC was orally administered. Therapeutic effect was evaluated with the Morris water maze test, FDG-PET examination, and histological examination, and the acting mechanism was studied with DNA microarrays and western blotting. Results CBJC treatment significantly attenuated IBO-induced abnormalities in cognition, brain functional images, and brain histological morphology. Additionally, the expression levels of 19 genes in the forebrain were significantly influenced by CBJC; approximately 60% of these genes were related to neuroprotection and neurogenesis, whereas others were related to anti-oxidation, protein degradation, cholesterol metabolism, stress response, angiogenesis, and apoptosis. Expression of these genes was increased, except for the gene related to apoptosis. Changes in expression for 5 of these genes were confirmed by western blotting. Conclusion CBJC can ameliorate the IBO-induced dementia in rats and may be significant in the treatment of AD. The therapeutic mechanism may be related to CBJC’s modulation of a number of processes, mainly through promotion of neuroprotection and neurogenesis, with additional promotion of anti-oxidation, protein degradation, etc.
Online Inspiration and Exploration for Identity Reinvention

Self-representation online can be difficult for those who are in life transitions that involve exploring new identity facets and changes in personal style. Many desire to tailor their online representations for different audiences. Social media site profiles and sharing settings offer varying levels of anonymity, privacy, and thus safety, but these settings are often opaque and poorly understood. To understand the complex relationship between identity, personal style and online self-representation, we examine how people explore and experiment with new styles in public and in private online settings during gender transition. We present the results of interviews with transgender people who have recently reinvented their personal style, or are planning to do so in the near future. We find that people explore new styles in online settings to craft possible or ideal future selves. When involving others, people engage intimate and unknown others, but often avoid weak ties. Our results indicate that to account for changing identities, social media sites must be designed to support finding inspiration and advice from strangers and style experimentation with close friends.
Recognition of nitrogen-responsive upstream activation sequences of Saccharomyces cerevisiae by the product of the GLN3 gene

We describe the purification of the product of the GLN3 gene of Saccharomyces cerevisiae and the demonstration that the purified product, Gln3p, binds specifically to the DNA sequences GATAAG and GATTAG, previously identified as nitrogen-responsive upstream activation sequences (UASN). When Gln3p is overproduced, it is released from the cells in a highly aggregated form incapable of specific binding to UASN. We used Gln3p tagged with six histidine codons at the 5' terminus and equipped with a galactose-inducible promoter to overproduce histidine-tagged Gln3p. The material was denatured, adsorbed to an Ni-nitrilotriacetic acid (NTA)-agarose column, eluted with imidazole, and after renaturation further purified on a gel filtration column. We then demonstrated the specific binding of the more than 90% pure Gln3p to the UASN by gel shift and footprinting methods.
Shape classification through structured learning of matching measures

Many traditional methods for shape classification involve establishing point correspondences between shapes to produce matching scores, which are in turn used as similarity measures for classification. Learning techniques have been applied only in the second stage of this process, after the matching scores have been obtained. In this paper, instead of simply taking for granted the scores obtained by matching and then learning a classifier, we learn the matching scores themselves so as to produce shape similarity scores that minimize the classification loss. The solution is based on a max-margin formulation in the structured prediction setting. Experiments in shape databases reveal that such an integrated learning algorithm substantially improves on existing methods.
“Beyond the Four Walls of My Building”: A Case Study of #Edchat as a Community of Practice

ABSTRACT Although Twitter and other social media sites have grown in popularity with educators, we still do not know what is happening within this online space or how it supports teachers. The purpose of this case study of #Edchat, a group of educators who meet weekly on Twitter, was to investigate informal professional development through the lens of communities of practice theory. Chat observations, participant interviews, and archival documents were analyzed. #Edchat reflected some aspects of a community of practice, including overlap in the description of who belongs, sustained mutual relationships, absences of introductory preamble in the conversations, quick setup of a problem to be discussed, and a rapid flow of information. The authors explore how these indicators overlap with best practices in professional development and recommend that administrators learn from what is happening in social media spaces as they work toward supporting teacher learning.
The E27 β2-adrenergic Receptor Polymorphism Reduces the Risk of Myocardial Infarction in Dyslipidemic Young Males

Summary In the present study we evaluated whether two polymorphisms of β2-adrenergic receptors (β2-AR) gene (R16G and Q27E) could modify the risk of myocardial infarction (MI). Using a case-control design, we analyzed the data from 125 male patients who had experienced a first episode of MI before the age of 45 years and 108 male controls matched for age. The allele frequencies for R16G and Q27E were: G16=0.56 and E27=0.36 in patients with MI and G16=0.61 and E27=0.42 in the control group. There was a trend (not statistically significant) of decreasing MI risk according to E27 or G16 alleles. Combined effect between E27 allele and history of dyslipidemia has been observed. Whereas dyslipidemia conferred a relative risk of MI of 4.8 (P<0.001) compared with normolipidemia in the entire study population, the relative risk increased to 9.0 (P<0.001) in Q27 homozygotes with dyslipidemia, and decreased to 1.8 (P=0.36) in E27 homozygotes. Our results show that the E27 allele of the β2-adrenergic receptor has a significant protective effect on MI in dyslipidemic young male.
The return of the Scarlet Pimpernel: cobalamin in inflammation II — cobalamins can both selectively promote all three nitric oxide synthases (NOS), particularly iNOS and eNOS, and, as needed, selectively inhibit iNOS and nNOS

The up-regulation of transcobalamins [hitherto posited as indicating a central need for cobalamin (Cbl) in inflammation], whose expression, like inducible nitric oxide synthase (iNOS), is Sp1- and interferondependent, together with increased intracellular formation of glutathionylcobalamin (GSCbl), adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), may be essential for the timely promotion and later selective inhibition of iNOS and concordant regulation of endothelial and neuronal NOS (eNOS/nNOS.) Cbl may ensure controlled high output of nitric oxide (NO) and its safe deployment, because: (1) Cbl is ultimately responsible for the synthesis or availability of the NOS substrates and cofactors heme, arginine, BH4 flavin adenine dinucleotide/flavin mononucleotide (FAD/FMN) and NADPH, via the far-reaching effects of the two Cbl coenzymes, methionine synthase (MS) and methylmalonyl CoA mutase (MCoAM) in, or on, the folate, glutathione, tricarboxylic acid (TCA) and urea cycles, oxidative phosphorylation, glycolysis and the pentose phosphate pathway. Deficiency of any of theNOS substrates and cofactors results in ‘uncoupled’ NOS reactions, decreasedNO production and increased or excessive O2−, H2O2, ONOO− and other reactive oxygen species (ROS), reactive nitric oxide species (RNIS) leading to pathology. (2) Cbl is also the overlooked ultimate determinant of positive glutathione status, which favours the formation of more benign NO species, s-nitrosothiols, the predominant form in which NO is safely deployed. Cbl status may consequently act as a ‘back-up disc’ that ensures the active status of antioxidant systems, as well as reversing and modulating the effects of nitrosylation in cell signal transduction.New evidence shows that GSCbl can significantly promote iNOS/ eNOS NO synthesis in the early stages of inflammation, thus lowering high levels of tumour necrosis factor-a that normally result in pathology, while existing evidence shows that in extreme nitrosative and oxidative stress, GSCbl can regenerate the activity of enzymes important for eventual resolution, such as glucose 6 phosphate dehydrogenase, which ensures NADPH supply, lactate dehydrogenase, and more; with human clinical case studies of OHCbl for cyanide poisoning, suggesting Cbl may regenerate aconitase and cytochrome c oxidase in the TCA cycle and oxidative phosphorylation. Thus, Cbl may simultaneously promote a strong inflammatory response and the means to resolve it.
Do Converts Always Make the Most Committed Christians

This paper presents an attempt to clarify the distinction between religious commitment and the identity change process through which an individual becomes religiously committed. It is argued that conversion and commitment have often been incorrectly equated in the social science literature. The question is asked whether those who have converted to a particular religious perspective are more committed than those who have been brought up in that tradition. The latter are referred to as alternators. Using a sample drawn from among born-again Christians, it is demonstrated here that religious commitment can be seen to vary within, but not between groups of converts and alternators. Religious commitment, it is argued, is sustained by interaction with other believers after recruitment to the religious group has taken place.
Iron, Iron‐binding Proteins and Immune System Cells a

In summary, the work reviewed in the present paper indicates that 1. Iron and the iron-binding proteins can act as regulators of immune function, and not only as a result of a nutritional dependence of lymphoid cells on transferrin and transferrin-iron. Subsets of cells of the immune system respond differently to increases in iron concentration in vitro and in vivo. 2. Macrophages and lymphocytes differ in the H and L subunit content of the ferritins synthesized in response to increases in iron concentration in vitro. 3. NK activity by adherent and nonadherent cells differ in their susceptibility to the enhancing effect of lactoferrin in vitro. 4. Responses to mitogen stimulation by PHA and Con A are diminished, while the PWM response remains unaffected by exposure to acidic ferritins or by increasing concentrations of iron in vitro and in vivo. 5. Pretreatment of effector but not target cells with iron results in diminished responses in the MLR, an effect that appears to be related to the HLA-A locus. 6. In situ hybridization studies indicate that transferrin is synthesized by a specific subset of the T lymphocytes. 7. Transient increases in serum iron concentration above the full saturation of transferrin, reproducing the clinical situation frequently seen in hereditary hemochromatosis, are followed by a series of cellular changes in the synovium that can be correlated to changes in the course of an experimental model of arthritis in the rat.
Study of basic-life-support training for college students.

OBJECTIVE To study about attitude and knowledge regarding basic-life-support among college students outside medical system. MATERIAL AND METHOD The cross-sectional study in the emergency department of Thammasat Hospital. The authors included college students at least aged 18 years old and volunteers to be study subjects. The authors collected data about attitudes and knowledge in performing basic-life-support by using set of questionnaires. RESULTS 250 college students participated in the two hours trainingprogram. Most ofparticipants (42.4%) were second-year college students, of which 50 of 250 participants (20%) had trained in basic-life-support program. Twenty-seven of 250 participants (10.8%) had experience in basic-life-support outside the hospital. Most of participants had good attitude for doing basic-life-support. Participants had a significant improved score following training (mean score 8.66 and 12.34, respectively, p<0.001). Thirty-three of 250 participants (13.2%) passed the minimum score before trained testing, whereas 170 of 250 participants (68%) passed the minimum score after trained testing. CONCLUSION With accurate knowledge and experience, lay rescuers may have more confidence tope7form basic-life-support to cardiac arrest patient. The training program in basic-life-support has significant impact on knowledge after training.
Hoarseness subsequent to cardiovascular surgery, intervention, maneuver and endotracheal intubation: the so-called iatrogenic Ortner's (cardiovocal) syndrome.

BACKGROUND The clinical characteristics and outcomes of hoarseness subsequent to cardiovascular surgery, intervention, maneuver and endotracheal intubation have not been systematically elucidated. METHODS The literature of hoarseness following cardiovascular surgery, intervention, and maneuver and intubation published between 1980 and 2011 was comprehensively retrieved in the MEDLINE database and the Google and Highwire Press search engines. RESULTS The so-called "iatrogenic Ortner's (cardiovocal) syndrome" developed 0-7 (2.33 ± ± 2.66) days following cardiovascular surgery, intervention, maneuver and endotracheal intubation with an incidence of 10.15%. The most common symptoms associated with hoarseness were stridor (49.45%) and aspiration (15.38%). Patent ductus arteriosus ligation and otherwise congenital heart disease repair were the two main causes leading to such a complication. Patients' hoarse voice spontaneously resolved in 70.52%, and persisted in 33.61% of the patients. Treatment of the hoarseness included gelfoam/teflon injection, intravenous steroid therapy, type 1 thyroplasty and arytenoid adduction. Hoarseness recovered in 46.67%, improved in 13.33%, and persisted in 40%. CONCLUSIONS The recurrent laryngeal nerve was often injured following cardiovascular surgery, intervention, maneuver and endotracheal intubation. Care must be taken during the manipulations in order to avoid the nerve injury. The so-called "iatrogenic Ortner's (cardiovocal) syndrome" was a wrong concept as it did not meet the satisfaction of a main element "cardiovascular disease as an underlying cause of hoarseness" of the definition of Ortner's (cardiovocal) syndrome defined by Ortner in 1897. It was actually an immediate vocal cord complication following cardiovascular manipulation.
The Power Structure of American Business.

Mintz and Schwartz offer a fascinating tour of the corporate world. Through an intensive study of interlocking corporate directorates, they show that for the first time in American history the loan making and stock purchasing and selling powers are concentrated in the same hands: the leadership of major financial firms. Their detailed descriptions of corporate case histories include the forced ouster of Howard Hughes from TWA in the late fifties as a result of lenders' pressure; the collapse of Chrysler in the late seventies owing to banks' refusal to provide further capital infusions; and the very different "rescues" of Pan American Airlines and Braniff Airlines by bank intervention in the seventies.