Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of human basic fibroblast growth factor cDNA in baby hamster kidney-derived cells results in autonomous cell growth

Growth factor over-production by responsive cells might contribute to their autonomous proliferation as well as their acquisition of a transformed phenotype in culture. Basic fibroblast growth factor (bFGF) has been shown to induce transient changes in cell behavior that resemble those encountered in transformed cells. In addition, several types of human tumor cells have been shown to produce bFGF. To determine directly the role that bFGF might play in the induction of the transformed phenotype, we have introduced a human bFGF cDNA expression vector into baby hamster kidney-derived (BHK-21) fibroblasts. One of the BHK transfectants, termed clone 19, expresses the bFGF mRNA and produces biologically active bFGF that accumulates to a high concentration inside the cells. These properties correlate with the ability of the cells to grow in serum-free medium without the addition of exogenous bFGF. Clone 19 cells also proliferated in soft agar, indicating that constitutive expression of the bFGF gene results in a loss of anchorage-dependent growth.
A comparison between Venables standardized respiratory questionnaire and pre-shift spirometry in screening of occupational asthma in a steel industry.

BACKGROUND Occupational asthma (OA) is the most common occupational lung disease in developed countries. One of the causative agents is metal fume that may be encountered in steel industries. Screening for the OA is mainly performed by questionnaire but in our country spirometry is used more commonly. OBJECTIVE To compare the diagnostic value of the Venables standardized respiratory questionnaire and pre-shift spirometry as screening tools for OA. METHODS In a cross-sectional study, we investigated 450 workers of a steel industry by the Venables standardized questionnaire. We also performed a pre-shift spirometry as the screening spirometry and a post-shift spirometry. A person with 10% drop in post-shift FEV1 compared with the pre-shift value was considered as asthmatic (our gold-standard). The results of the questionnaire and the pre-shift spirometry were then examined against the gold-standard test results. For each test, sensitivity, specificity, positive and negative predictive values were calculated. RESULTS The overall prevalence of OA among our studied workers was 3.9% (95% CI: 1.9%-5.9%). The highest rate was seen in those working in catering (25%) and welding (10%) units. Pre-shift spirometry and the questionnaire had low sensitivity (42.9% and 28.6%, respectively) and positive predictive values (16.7% and 3.6%, respectively); moderate specificity (92.4% and 71.6%, respectively) and high negative predictive values (97.9% and 96.5%, respectively). CONCLUSION Taking into account the ease of use of the questionnaire, it seems that it is more feasible to use questionnaire as the primary screening tool for the diagnosis of OA.
Metabolism in 1,3‐propanediol fed‐batch fermentation by a D‐lactate deficient mutant of Klebsiella pneumoniae

Klebsiella pneumoniae HR526, a new isolated 1,3‐propanediol (1,3‐PD) producer, exhibited great productivity. However, the accumulation of lactate in the late‐exponential phase remained an obstacle of 1,3‐PD industrial scale production. Hereby, mutants lacking D‐lactate pathway were constructed by knocking out the ldhA gene encoding fermentative D‐lactate dehydrogenase (LDH) of HR526. The mutant K. pneumoniae LDH526 with the lowest LDH activity was studied in aerobic fed‐batch fermentation. In experiments using pure glycerol as feedstock, the 1,3‐PD concentrations, conversion, and productivity increased from 95.39 g L−1, 0.48 and 1.98 g L−1 h−1 to 102. 06 g L−1, 0.52 mol mol−1 and 2.13 g L−1 h−1, respectively. The diol (1,3‐PD and 2,3‐butanediol) conversion increased from 0.55 mol mol−1 to a maximum of 0.65 mol mol−1. Lactate would not accumulate until 1,3‐PD exceeded 84 g L−1, and the final lactate concentration decreased dramatically from more than 40 g L−1 to <3 g L−1. Enzymic measurements showed LDH activity decreased by 89–98% during fed‐batch fermentation, and other related enzyme activities were not affected. NADH/NAD+ enhanced more than 50% in the late‐exponential phase as the D‐lactate pathway was cut off, which might be the main reason for the change of final metabolites concentrations. The ability to utilize crude glycerol from biodiesel process and great genetic stability demonstrated that K. pnemoniae LDH526 was valuable for 1,3‐PD industrial production. Biotechnol. Bioeng. 2009; 104: 965–972. © 2009 Wiley Periodicals, Inc.
Chromosome 2 aberrations in clinical cases characterised by high resolution multicolour banding and region specific FISH probes

The field of human cytogenetics has been through many different stages of development, each of them improving the characterisation of structurally abnormal and/or supernumerary chromosomes. The era of reliable identification of human chromosomes started with the invention of the banding method by Dr Lore Zech in 1968.1 The introduction of fluorescence in situ hybridisation (FISH) techniques in human cytogenetics by Pinkel et al 2 in 1986 allowed specific staining of chromosomes and chromosomal subregions. Even though G banding3 is still the gold standard against which all molecular cytogenetic techniques are measured, this technique based on alternating light and dark bands can lead to equivocal chromosome breakpoints.4 The development of multicolour FISH5 in 1996, multiplex FISH (M-FISH),6 and spectral karyotyping (SKY),7 allowing the simultaneous and specific painting of all 24 human chromosomes in different colours, was helpful in overcoming these problems in part. However, they are not suited for the detection of inversions or duplications or for more precise determination of chromosome breakpoints. Several FISH based techniques that are capable of solving this problem have been developed in the last decade: the application of chromosome arm specific probes,6,8 the use of chromosome bar codes,9,10 the cross species colour banding (RX-FISH) approach,11 and the high resolution multicolour banding technique (MCB). 12,13 The latter approach can cover the entire karyotype with human DNA probes without leaving any gaps. To illustrate the power of the MCB technique, clinical cases with five different kinds of aberrations identified by conventional banding techniques, that is, translocations (four cases), deletions (two cases), duplications (three cases), inversions (two cases), and small supernumerary marker chromosomes (one case), were reinvestigated. In 9/11 cases (∼80%), the chromosome breakpoints were redefined by MCB and these results have been confirmed by locus …
A Phase Ib/II Study Evaluating the Combination of Weekly Docetaxel and Cisplatin Together with Capecitabine and Bevacizumab in Patients with Advanced Esophago-Gastric Cancer

Introduction Current treatment options for advanced esophagogastric cancer (AEGC) are still unsatisfactory. The aim of this prospective phase Ib/II study was to evaluate the safety and efficacy of a novel regimen, AVDCX, consisting of weekly docetaxel and cisplatin together with capecitabine and bevacizumab, in AEGC. Methods Patients with AEGC received treatment with different dose levels of AVDCX (cisplatin and docetaxel 25–35 mg/m2, days 1,8, capecitabine 1,600 mg/m2 days 1–14, bevacizumab 7.5 mg/kg, day 1, Q:21 days). The study's primary objectives were to establish the recommended phase II doses of docetaxel and cisplatin in AVDCX (phase Ib part) and to determine the tumor response rate (phase II part). Results The study was closed early, after the accrual of 22 patients, due to accumulating toxicity-related deaths. The median age was 59 years and 77% of patients had gastric or gastroesophageal adenocarcinomas. Grade ≥3 adverse events were documented in 18 patients (82%), usually neutropenia (36%), fatigue (54%) or diarrhea (23%). There were three fatal toxicities (14%): mesenteric thromboembolism, gastric perforation and pancytopenic sepsis. The recommended phase II doses of cisplatin and docetaxel were determined to be 25 mg/m2 and 30 mg/m2, respectively. Twenty-one patients were evaluable for response: 12 (54%) had partial response (PR), 4 (18%) had stable disease (SD) and none had complete response (CR). Hence, the objective response rate (CR+PR) was 54% and the disease control rate (CR+PR+SD) was 72%. For the 17 patients treated at the MTD, the objective response rate was 41% and the disease control rate was 88%. The median overall survival (OS) for these patients was 13.9 months (range, 1.5–52.2 months) and the median progression-free survival was 7.6 months (range, 1.3–26.6 months). The 2-year OS rate reached 23.7%. Conclusions AVDCX was associated with a high rate of regimen related fatal adverse events and is not appropriate for further development in AEGC patients. Trial Registration ClinicalTrials.gov NCT00845884,
Generic Language in Parent-Child Conversations

Generic knowledge concerns kinds of things (e.g., birds fly; a chair is for sitting; gold is a metal). Past research demonstrated that children spontaneously develop generic knowledge by preschool age. The present study examines when and how children learn to use the multiple devices provided by their language to express generic knowledge. We hypothesize that children assume, in the absence of specifying information or context that nouns refer to generic kinds, as a default. Thus, we predict that (a) children should talk about kinds from an early age; (b) children should learn generic forms with only minimal parental scaffolding; (c) children should recognize a variety of different linguistic forms as generic. Results from longitudinal samples of adult-child conversations support all three hypotheses. We also report individual differences in the use of generics, suggesting that children differ in their tendency to form the abstract generalizations so expressed.
Fixed-Parameter Algorithms for Finding Minimal Models

Computing minimal models is an important task in Knowledge Representation and Reasoning that appears in formalisms such as circumscription, diagnosis and answer set programming. Even the most basic question of whether there exists a minimal model containing a given variable is known to be ΣP2-complete. In this work we study the problem of computing minimal models from the viewpoint of parameterized complexity theory. We perform an extensive complexity analysis of this problem with respect to eleven parameters. Tractable fragments based on combinations of these parameters are identified by giving several fixed-parameter algorithms. For the remaining combinations we show parameterized hardness results and thus prove that under usual complexity theoretic assumptions no further fixed-parameter algorithms exist for these parameters.
Loss of DJ-1 Does Not Affect Mitochondrial Respiration but Increases ROS Production and Mitochondrial Permeability Transition Pore Opening

Background Loss of function mutations in the DJ-1 gene have been linked to recessively inherited forms of Parkinsonism. Mitochondrial dysfunction and increased oxidative stress are thought to be key events in the pathogenesis of Parkinson’s disease. Although it has been reported that DJ-1 serves as scavenger for reactive oxidative species (ROS) by oxidation on its cysteine residues, how loss of DJ-1 affects mitochondrial function is less clear. Methodology/Principal Findings Using primary mouse embryonic fibroblasts (MEFs) or brains from DJ-1−/− mice, we found that loss of DJ-1 does not affect mitochondrial respiration. Specifically, endogenous respiratory activity as well as basal and maximal respiration are normal in intact DJ-1−/− MEFs, and substrate-specific state 3 and state 4 mitochondrial respiration are also unaffected in permeabilized DJ-1−/− MEFs and in isolated mitochondria from the cerebral cortex of DJ-1−/− mice at 3 months or 2 years of age. Expression levels and activities of all individual complexes composing the electron transport system are unchanged, but ATP production is reduced in DJ-1−/− MEFs. Mitochondrial transmembrane potential is decreased in the absence of DJ-1. Furthermore, mitochondrial permeability transition pore opening is increased, whereas mitochondrial calcium levels are unchanged in DJ-1−/− cells. Consistent with earlier reports, production of reactive oxygen species (ROS) is increased, though levels of antioxidative enzymes are unaltered. Interestingly, the decreased mitochondrial transmembrane potential and the increased mitochondrial permeability transition pore opening in DJ-1−/− MEFs can be restored by antioxidant treatment, whereas oxidative stress inducers have the opposite effects on mitochondrial transmembrane potential and mitochondrial permeability transition pore opening. Conclusions/Significance Our study shows that loss of DJ-1 does not affect mitochondrial respiration or mitochondrial calcium levels but increases ROS production, leading to elevated mitochondrial permeability transition pore opening and reduced mitochondrial transmembrane potential.
Review Article Autologous Stem Cell Transplant for Al Amyloidosis

AL amyloidosis is caused by clonal plasma cells that produce immunoglobulin light chains which misfold and get deposited as amyloid fibrils. Therapy directed against the plasma cell clone leads to clinical benefit. Melphalan and corticosteroids have been the mainstay of treatment for a number of years and the recent availability of other effective agents (IMiDs and proteasome inhibitors) has increased treatment options. Autologous stem cell transplant (ASCT) has been used in the treatment of AL amyloidosis for many years. It is associated with high rates of hematologic response and improvement in organ function. However, transplant carries considerable risks. Careful patient selection is important to minimize transplant related morbidity and mortality and ensure optimal patient outcomes. As newer more affective therapies become available the role and timing of ASCT in the overall treatment strategy of AL amyloidosis will need to be continually reassessed.
Rational Diverse Beliefs and Economic Volatility

This paper explores works which contribute to explaining market dynamics and volatility by employing models of rational but diverse beliefs. The first part gives a complete exposition of Noisy Rational Expectations (i.e. REE) Asset Pricing Theory where diversity of beliefs arises from diverse private information. The general conclusion is that, although Noisy REE asset pricing theories have many attractive features, they do not offer a satisfactory paradigm for market dynamics. Indeed, for most models increased amount of idiosyncratic private information leads to a reduction in volatility. We next turn to models of diverse beliefs without private information. We explore work on Rational Belief Equilibria (i.e. RBE) starting with modeling beliefs. It is shown that to be rational a belief cannot be constant, creating an endogenous mechanism for market dynamics. For the development of an equilibrium theory, beliefs are treated as a vector of state variables and market belief is the distribution of individual beliefs. Market belief is an observable for which ample data is available and we review both data sources and applications which use such data. Much of the material reviewed covers the applications of this theory to the study of market dynamics.
Fractional embedding of differential operators and Lagrangian systems

This paper is a contribution to the general program of embedding theories of dynamical systems. Following our previous work on the stochastic embedding theory developed with Darses [C. R. Acad. Sci. Ser. I: Math 342, 333 (2006); (preprint IHES 06/27, p. 87, 2006)], we define the fractional embedding of differential operators and ordinary differential equations. We construct an operator combining in a symmetric way the left and right (Riemann-Liouville) fractional derivatives. For Lagrangian systems, our method provides a fractional Euler-Lagrange equation. We prove, developing the corresponding fractional calculus of variations, that such equation can be derived via a fractional least-action principle. We then obtain naturally a fractional Noether theorem and a fractional Hamiltonian formulation of fractional Lagrangian systems. All these constructions are coherents, i.e., the embedding procedure is compatible with the fractional calculus of variations. We then extend our results to cover the Ostrogradski.
What Makes Lawyers Happy?: A Data-Driven Prescription to Redefine Professional Success

This is the first theory-guided empirical research seeking to identify the correlates and contributors to the well-being and life satisfaction of lawyers. Data from several thousand lawyers in four states provide insights about diverse factors from law school and one's legal career and personal life. Striking patterns appear repeatedly in the data and raise serious questions about the common priorities on law school campuses and among lawyers. External factors, which are often given the most attention and concern among law students and lawyers (factors oriented towards money and status — such as earnings, partnership in a law firm, law school debt, class rank, law review membership, and U.S. News & World Report's law school rankings), showed nil to small associations with lawyer well-being. Conversely, the kinds of internal and psychological factors shown in previous research to erode in law school appear in these data to be the most important contributors to lawyers’ happiness and satisfaction. These factors constitute the first two of five tiers of well-being factors identified in the data, followed by choices regarding family and personal life. The external money and status factors constitute the fourth tier, and demographic differences were least important.Data on lawyers in different practice types and settings demonstrate the applied importance of the contrasting internal and external factors. Attorneys in large firms and other prestigious positions were not as happy as public service attorneys, despite the far better grades and pay of the former group; and junior partners in law firms were no happier than senior associates, despite the greatly enhanced pay and status of the partners. Overall, the data also demonstrate that lawyers are very much like other people, notwithstanding their specialized cognitive training and the common perception that lawyers are different from others in fundamental ways.Additional measures raised concerns. Subjects did not broadly agree that the behavior of judges and lawyers is professional, or that the legal process reaches fair outcomes; and subjects reported quite unrealistic earnings expectations for their careers when they entered law school. Implications for improving lawyer performance and professionalism, and recommendations for law teachers and legal employers, are drawn from the data.
Effects of Mitochondrial Translocation of Telomerase on Drug Resistance in Hepatocellular Carcinoma Cells

Hepatocellular carcinoma (HCC) cells exhibit multidrug resistance (MDR), but the underlying mechanisms remain unclear. Cancer cells that overexpress telomerase are resistant to chemotherapeutic drugs. This study aimed to determine the effects of mitochondrial translocation of telomerase on MDR in HCC cells. HepG2 cells were transfected with negative plasmid and PTPN11 (Shp-2) short hairpin RNA (ShRNA) plasmid to establish HepG2-negative (HepG2 transfected with negative plasmid) and HepG2-ShShp-2 (HepG2 transfected with Shp-2 ShRNA plasmid) cells. Sensitivity to chemotherapeutic drugs was assessed by Cell Counting Kit-8 (CCK-8) assays. Distribution of human telomerase reverse transcriptase (hTERT) within mitochondria was detected by western blotting and immunofluorescence combined with laser scanning confocal microscopy. Mitochondrial reactive oxygen species (ROS) generation was demonstrated by flow cytometry with the mitochondrial superoxide (Mito-Sox) indicator. The frequency of damaged mitochondrial DNA (mtDNA) was illustrated by quantitative real-time polymerase chain reaction (Q-PCR). Expression of mitochondrial respiratory chain complex subunits ND1 and COXII were also demonstrated by western blotting. Knockdown of Shp-2 in HepG2 cells resulted in upregulation of mitochondrial TERT expression and increased resistance to cisplatin (CDDP) and 5-fluorouracil (5-FU) (resistance indices, 2.094 and 1.863, respectively). In addition, both the mitochondrial ROS and the frequency of mtDNA damage were decreased, and COXII expression was upregulated. Our results suggest that Mitochondrial translocation of hTERT may lead to chemotherapeutic resistance in HCC cells. Mitochondrial hTERT contributes to the drug resistance of tumor cells by reducing ROS production and mtDNA damage, and exerting a protective effect on the mitochondrial respiratory chain.
Modelling and identification of the da Vinci Research Kit robotic arms

The da Vinci Research Kit (DVRK) is a telerobotic surgical research platform endowed with an open controller that allows position, velocity and current control. We consider the problem of modelling and identification of both the Patient Side Manipulators (PSMs) and of the Master Tool Manipulators (MTMs) of the platform. This problem is relevant when realistic dynamic simulations have to be performed using standard software tools, but also for the design of model-based control laws, and for the implementation of sensorless strategies for collision detection or contact force estimation. A LMI-based approach is used for the identification of the robot dynamics in order to guarantee the physical feasibility of the parameters that is not ensured by standard least-squares methods. The identified models are validated experimentally.
Interaction between parasympathetic and sympathetic nerves in prevertebral ganglia: Morphological evidence for vagal efferent innervation of ganglion cells in the rat

Vagal efferent preganglionic neurons were anterogradely labeled by injecting either DiI or DiA, fluorescent lipophilic carbocyanine dyes, into the dorsal motor nucleus of the vagus of the rat. All neurons of the peripheral nervous system (outside the blood‐brain barrier) were then fluorescently counterstained in vivo by injecting Fluorogold (Fluorochrome, Inc., Englewood, CO) intraperitoneally. The upper abdominal prevertebral ganglia, including the numerous microganglia associated with the periarterial plexuses of the celiac and superior mesenteric arteries, were identified and dissected in formalin‐fixed tissue under ultraviolet light and stereomicroscopic guidance. In 14 of 15 animals analyzed (93%), labeled vagal efferent fibers were found to penetrate into both the left and right celiac ganglia and the superior mesenteric ganglion, as well as into some of the associated microganglia. These projections formed varicose terminal‐like structures, highly suggestive of synaptic contacts surrounding individual ganglion cells. In about half the animals, such vagal innervation was also seen in the left and right suprarenal ganglia. The specificity of vagal efferent labeling was confirmed by control experiments, which included injections in vagotomized animals and direct selective labeling of vagal afferents from the nodose ganglia. It is concluded that vagal efferent preganglionics innervate principal ganglion cells of prevertebral ganglia. These vagal contacts may either directly modulate the postganglionic outflow or else gate some or all of the potential modulatory inputs to these postganglionic neurons, thus allowing the vagal system to exert a more selective influence on sympathetic outflow. Finally, the use of laser scanning confocal microscopy and the in toto Fluorogold staining method for investigations of the peripheral nervous system are discussed. © 1996 Wiley‐Liss, Inc.