Miracle-dz/UniFAR-FTUs huggingface dataset:
Hydrogen Sulfide Regulates Krüppel‐Like Factor 5 Transcription Activity via Specificity Protein 1 S‐Sulfhydration at Cys664 to Prevent Myocardial Hypertrophy

Background Hydrogen sulfide (H2S) is a gasotransmitter that regulates multiple cardiovascular functions. Krüppel‐like factor 5 (KLF5) exerts diverse functions in the cardiovascular system. Whether and how H2S regulates KLF5 in myocardial hypertrophy is unknown. Methods and Results In our study, hypertrophic myocardial samples in the clinic were collected and underwent histological and molecular biological analysis. Spontaneously hypertensive rats and neonatal rat cardiomyocytes were studied for functional and signaling responses to GYY4137, an H2S‐releasing compound. Expression of cystathionine γ‐lyase, a principal enzyme for H2S generation in heart, decreased in human hypertrophic myocardium, whereas KLF5 expression increased. After GYY4137 administration for 4 weeks, myocardial hypertrophy was inhibited in spontaneously hypertensive rats, as demonstrated by improvement in cardiac structural parameters, heart mass, size of cardiac myocytes, and expression of atrial natriuretic peptide. H2S diminished expression of KLF5 in myocardium of spontaneously hypertensive rats and in hypertrophic cardiomyocytes. H2S also inhibits platelet‐derived growth factor A promoter activity, decreased recruitment of KLF5 to the platelet‐derived growth factor A promoter, and reduced atrial natriuretic peptide expression in angiotensin II–stimulated cardiomyocytes, and these effects are suppressed by KLF5 knockdown. KLF5 promoter activity and KLF5 expression was also reversed by H2S. H2S increased the S‐sulfhydration on specificity protein 1 in cardiomyocytes. Moreover, H2S decreased KLF5 promoter activity; reduced KLF5 mRNA expression; attenuated specificity protein 1 binding activity with KLF5 promoter; and inhibited hypertrophy after specificity protein 1 mutated at Cys659, Cys689, and Cys692 but not Cys664 overexpression. Conclusions These findings suggest that H2S regulates KLF5 transcription activity via specificity protein 1 S‐sulfhydration at Cys664 to prevent myocardial hypertrophy.
Breast Carcinomatous Tumoral Emboli Can Result From Encircling Lymphovasculogenesis Rather Than Lymphovascular Invasion

The canonical view of the origin of tumor lymphovascular emboli is that they usually originate from lymphovascular invasion as part of a multistep metastatic process. Recent experimental evidence has suggested that metastasis can occur earlier than previously thought and we found evidence that tumor emboli formation can result from the short-circuiting step of encircling lymphovasculogenesis. Experimentally, we used a xenograft of human inflammatory breast cancer (MARY-X), a model that exhibited florid tumor emboli, to generate tumoral spheroids in vitro. In observational studies, we chose human breast carcinoma cases where there appeared to be a possible transition of in situ carcinoma to lymphovascular emboli without intervening stromal invasion. These cases were studied by morphometry as well as IHC with tumor proliferation (Ki-67) and adhesion (E-cadherin) markers, myoepithelial (p63), as well as endothelial (podoplanin [D2-40], CD31, VEGFR-3, Prox-1) markers. Unlabelled spheroids coinjected with either GFP or RFP-human myoepithelial cells or murine embryonal fibroblasts (MEFs) gave rise to tumors which exhibited GFP/RFP immunoreactivity within the cells lining the emboli-containing lymphovascular channels. In vitro studies demonstrated that the tumoral spheroids induced endothelial differentiation of cocultured myoepithelial cells and MEFs, measured by real time PCR and immunofluorescence. In humans, the in situ clusters exhibited similar proliferation, E-cadherin immunoreactivity and size as the tumor emboli (p =.5), suggesting the possibility that the latter originated from the former. The in situ clusters exhibited a loss (50%-100%) of p63 myoepithelial immunoreactivity but not E-cadherin epithelial immunoreactivity. The tumor emboli were mainly present within lymphatic channels whose dual p63/CD31, p63/D2-40 and p63/VEGFR-3 and overall weak patterns of D2-40/CD31/VEGFR-3 immunoreactivities suggested that they represented immature and newly created vasculature derived from originally myoepithelial-lined ducts. Collectively both experimental as well as observational studies suggested the possibility that these breast cancer emboli resulted from encircling lymphovasculogenesis rather than conventional lymphovascular invasion.
Adherence to Vitamin D Recommendations Among US Infants

OBJECTIVES: In November 2008, the American Academy of Pediatrics (AAP) doubled the recommended daily intake of vitamin D for infants and children, from 200 IU/day (2003 recommendation) to 400 IU/day. We aimed to assess the prevalence of infants meeting the AAP recommended intake of vitamin D during their first year of life. METHODS: Using data from the Infant Feeding Practices Study II, conducted from 2005 to 2007, we estimated the percentage of infants who met vitamin D recommendations at ages 1, 2, 3, 4, 5, 6, 7.5, 9, and 10.5 months (n = 1952–1633). RESULTS: The use of oral vitamin D supplements was low, regardless of whether infants were consuming breast milk or formula, ranging from 1% to 13%, varying by age. Among infants who consumed breast milk but no formula, only 5% to 13% met either recommendation. Among mixed-fed infants, 28% to 35% met the 2003 recommendation, but only 9% to 14% would have met the 2008 recommendation. Among those who consumed formula but no breast milk, 81% to 98% met the 2003 recommendation, but only 20% to 37% would have met the 2008 recommendation. CONCLUSIONS: Our findings suggest that most US infants are not consuming adequate amounts of vitamin D according to the 2008 AAP recommendation. Pediatricians and health care providers should encourage parents of infants who are either breastfed or consuming <1 L/day of infant formula to give their infants an oral vitamin D supplement.
Effects of Presynaptic Mutations on a Postsynaptic Cacna1s Calcium Channel Colocalized with Mglur6 at Mouse Photoreceptor Ribbon Synapses

PURPOSE. Photoreceptor ribbon synapses translate light-dependent changes of membrane potential into graded transmitter release via L-type voltage-dependent calcium channel (VDCC) activity. Functional abnormalities (e.g., a reduced electroreti-nogram b-wave), arising from mutations of presynaptic proteins , such as Bassoon and the VDCC␣1 subunit Cacna1f, have been shown to altered transmitter release. L-type VDCC␣1 subtype expression in wild-type and mutant mice was examined , to investigate the underlying pathologic mechanism. METHODS. Two antisera against Cacna1f, and a Cacna1f mouse mutant (Cacna1f⌬Ex14-17) were generated. Immunocyto-chemistry for L-type VDCC␣1 subunits and additional synaptic marker proteins was performed in wild-type, Bassoon⌬Ex4-5 and Cacna1f⌬Ex14-17 mice. RESULTS. Active zone staining at photoreceptor ribbon synapses with a pan␣1 antibody colocalized with staining for Cacna1f in wild-type mouse retina. Similarly, in the Bassoon⌬Ex4-5 mouse, residual mislocalized staining for pan␣1 and Cacna1f showed colocalization. Unlike the presynaptic location of Cacna1f and pan␣1 antibody staining, the skeletal muscle VDCC␣1 subunit Cacna1s was present postsynaptically at ON-bipolar cell dendrites, where it colocalized with metabotropic glutamate receptor 6 (mGluR6). Surprisingly, Cacna1s labeling was severely downregulated in the Bassoon⌬Ex4-5 and Cacna1f⌬Ex14-17 mutants. Subsequent analyses revealed severely reduced ON-bipolar cell dendritic expression of the sarcoplasmic reticulum Ca 2ϩ ATPase Serca2 in both mouse mutants and of mGluR6 in the Cacna1f⌬Ex14-17 mutant. CONCLUSIONS. Presynaptic mutations leading to reduced photo-receptor-to-bipolar cell signaling are associated with disturbances in protein expression within postsynaptic dendrites. Moreover, detection of Cacna1s and Serca2 in ON-bipolar cell dendrites in wild-type animals suggests a putative role in regulation of postsynaptic Ca 2ϩ flux. P hotoreceptor ribbon synapses continuously translate light-dependent changes in membrane potential into graded release of transmitter. Several features of ribbon synapses reflect the functional requirement for high rates of tonic transmitter release and for transmission of signals over several orders of magnitude. 1,2 For example, the calcium-triggered fusion of neurotransmitter-filled vesicles at ribbon synapses is mediated by slowly inactivating L-type voltage-dependent calcium channels (VDCCs), rather than by the Nor P/Q-type channels present in conventional synapses. 3–7 The central, pore-forming component of a VDCC is provided by the ␣1 subunit. Of the 10 known VDCC␣1 subunits, four form L-type channels: Cacna1s (Ca v 1.1), Cacna1c (Ca v 1.2), Cacna1d (Ca v 1.3), and Cacna1f (Ca v 1.4). 7 Loss-of-function mutations in the human CACNA1F gene cause the incomplete form of X-linked congenital stationary night blindness (CSNB2). 8,9 The condition is characterized by an electroretino-gram (ERG) with a largely intact a-wave and a …
Geographical Mobility of Danish Dual-Earner Couples: The Relationship between Change of Job and Change of Residence

We analyze the relationship between geographical residence and job mobility for Danish dual-earner couples. We estimate the probability of moving residence and changing job, taking the interdependence between the events into account. The results point to the importance of addressing the interrelationship between residence and jobs. Furthermore, the change of residence matter more than change of job and women respond relatively more to changes in their husbands’ job region. The findings imply that mobility promoting initiatives must focus on families rather than individuals and recognize that for most families the choice of residence location dominates the choice of job location.
Views of Maharashtrian Postgraduates about Dowry, Divorce, Remarriage and Singlehood

Three hundred Maharashtrian postgraduate students (21-26 yrs) were chosen at random from purposely selected seven colleges of Parbhani town, Maharashtra state. Seventy two percent of the sample PG students hailed from middle SES group and the remaining 23 percent from low SES. The data was collected personally interviewing the PG students based on the interview schedule cum checklist. Majority of the PG boys and girls expressed unfavourable views about taking or giving dowry in marriage. Significantly a higher percentage of the Maharashtrian PG girls reported positive views about divorce for valid reasons as compared to their male counterparts. Majority of them had favourable views about remarriage of divorced / widow / widower for the reason of preventing problems of singlehood and to have a partner to lead a happy life and majority of them have not favoured singlehood for the reasons difficult to live without a companion and exploitation of self by people. Significant differences were found in their views based on SES and gender.
Clustering via the Bayesian information criterion with applications in speech recognition

One difficult problem we are often faced with in clustering analysis is how to choose the number of clusters. We propose to choose the number of clusters by optimizing the Bayesian information criterion (BIC), a model selection criterion in the statistics literature. We develop a termination criterion for the hierarchical clustering methods which optimizes the BIC criterion in a greedy fashion. The resulting algorithms are fully automatic. Our experiments on Gaussian mixture modeling and speaker clustering demonstrate that the BIC criterion is able to choose the number of clusters according to the intrinsic complexity present in the data.
Investigating the Safety of Electroacupuncture with a Picoscope™

Our wish to know more about the paths taken by electrical currents in electroacupuncture (EA) with special reference to the heart, particularly in patients with an implanted pacemaker, prompted us to undertake this study. Using ourselves as subjects, we have developed a safe oscillographic method to detect, visualise and record the EA currents that avoids the use of equipment requiring mains electricity. After two trials with unsatisfactory equipment, we found that the newly developed model 3425 PicoScope™ (Pico Technology Ltd), with a four channel differential amplifier input connected to a laptop PC operating in battery mode, satisfied our criteria. With this recording system, we carried out two sets of experiments in which EA was provided by a Cefar acus4™ stimulator. The results confirm that the placement of a pair of acupuncture needles for EA can be used to predict the paths taken by the stimulating currents, and thus their areas of likely influence. When the needles are placed in closely adjacent acupuncture points in a limb, there is little or no detectable spread of the currents along the limb or into the chest. By contrast, when the needles are placed far apart, the electrical currents spread widely. Thus, when each of a pair of needles is placed in a point on opposite arms, the electrical currents recorded in the area of the pectoral muscles is of an order that might trigger an abnormal cardiac rhythm in a susceptible heart or activate a cardiac pacemaker incorporating an intracardiac defibrillator (ICD). Our results confirm the guidelines for EA safe practice recommended by the British Medical Acupuncture Society (BMAS) to avoid adverse events, ie EA should not be applied such that the current is likely to traverse the heart. We can now be confident that electric fields generated by pairs of needles below the knee or elbow do not create detectable currents in the chest. It is likely that similar results would be obtained with the use of transcutaneous electrical nerve stimulation (TENS) but this remains to be established by additional experiments.
Evaluating expertise recommendations

Finding a person who has the expertise to solve a specific problem is an important application of recommender systems to a difficult organizational problem. Prior systems have made attempts to implement solutions to this problem, but few systems have undergone systematic user evaluation. This work describes a systematic evaluation of the Expertise Recommender (ER), a system that recommends people who are likely to have expertise in a specific problem. ER and the organizational context for which it was designed are described to provide a basis for understanding this evaluation. Prior to conducting the evaluation, a baseline experiment showed that people are relatively good at judging coworkers' expertise when given an appropriate context. This finding provides a way to demonstrate the effectiveness of ER by comparing ER's performance to ratings by coworkers. The evaluation, the design, and results are described in detail. The results suggest that the participants agree with the recommendations made by ER, and that ER significantly outperforms other expertise recommender systems when compared using similar metrics.
Density profile of pyrolite under the lower mantle conditions

The pyrolite model is one of the possible compositions of the Earth's lower mantle. The lower mantle's composition is generally modelled by comparing seismic observations with mineral physics data of possible lower mantle end‐member phases. Here, we report the compression behavior of a natural KLB‐1 peridotite (a representative composition of the pyrolite model) in a quasi‐hydrostatic environment at simultaneous high pressure (P) and temperature (T), covering the entire range of lower mantle P‐T conditions up to 112 GPa. This is the first experimentally determined density profile of pyrolite under the lower mantle conditions. The results allow us to directly compare the measured density of peridotite mantle along the geotherm with the Preliminary Reference Earth Model (PREM) derived from seismic observations, without extrapolation. The comparison shows significant mismatch between the two, which calls for a re‐evaluation of the PREM density model or a non‐pyrolite lower mantle composition.
Characteristics of Australian B2B iMarketplaces

Existing research on B 2B iMarketplaces (and intermediaries operating them) focuses primarily on viewing US iMarketplace web sites or conducting case studies. This paper extends this work by presenting survey findings of the total identifiable population of Australian B2B iMarketplace intermediaries to describe the iMarketplace characteristics and to determine if the findings provide more generalisable support for the literature.
A Theory of the Theory of Public Goods

A public good, as defined by economic theory, is a good that, once produced, can be consumed by an additional consumer at no additional cost. A second characteristic is sometimes added, specifying that consumers cannot be excluded from consuming the public good once it is produced. Goods with these characteristics will be underproduced in the private sector, or may not be produced at all, following the conventional wisdom, so economic efficiency requires that the government force people to contribute to the production of public goods, and then allow all citizens to consume them. Simple observation of the real world suggests two problems with the application of public goods theory as a justification for government production. First, many public goods are successfully produced in the private sector, so government production is not necessary. Second, many of the goods government actually does produce do not correspond to the economist's definition of public goods, so the theory does a poor job of explaining the government's actual role in the economy. If public goods theory fails as a theory of public expenditure, why is it so firmly entrenched in the economic theory of the public sector? This paper develops a theory to explain the development and use of public goods theory as a justification for government production. The paper begins by examining the theory of public goods. Public goods certainly exist, in the sense that there are goods that fit the economist's definition of public goods, but production in the public sector is neither necessary nor sufficient for the efficient production of public goods. A model that explains government involvement in the economy is then presented. Within this model, the production of national defense is explained as an institution that enables the government to protect and enhance its own wealth. Following this reasoning, national defense is produced by government because it furthers the private interests of those who run the government, not because it is in the public interest for the government to produce public goods. The model in this paper has more
Dropout: a simple way to prevent neural networks from overfitting

Deep neural nets with a large number of parameters are very powerful machine learning systems. However, overfitting is a serious problem in such networks. Large networks are also slow to use, making it difficult to deal with overfitting by combining the predictions of many different large neural nets at test time. Dropout is a technique for addressing this problem. The key idea is to randomly drop units (along with their connections) from the neural network during training. This prevents units from co-adapting too much. During training, dropout samples from an exponential number of different "thinned" networks. At test time, it is easy to approximate the effect of averaging the predictions of all these thinned networks by simply using a single unthinned network that has smaller weights. This significantly reduces overfitting and gives major improvements over other regularization methods. We show that dropout improves the performance of neural networks on supervised learning tasks in vision, speech recognition, document classification and computational biology, obtaining state-of-the-art results on many benchmark data sets.
Comparing a Perceptual and an Automated Vision-Based Method for Lie Detection in Younger Children

The present study investigates how easily it can be detected whether a child is being truthful or not in a game situation, and it explores the cue validity of bodily movements for such type of classification. To achieve this, we introduce an innovative methodology – the combination of perception studies (in which eye-tracking technology is being used) and automated movement analysis. Film fragments from truthful and deceptive children were shown to human judges who were given the task to decide whether the recorded child was being truthful or not. Results reveal that judges are able to accurately distinguish truthful clips from lying clips in both perception studies. Even though the automated movement analysis for overall and specific body regions did not yield significant results between the experimental conditions, we did find a positive correlation between the amount of movement in a child and the perception of lies, i.e., the more movement the children exhibited during a clip, the higher the chance that the clip was perceived as a lie. The eye-tracking study revealed that, even when there is movement happening in different body regions, judges tend to focus their attention mainly on the face region. This is the first study that compares a perceptual and an automated method for the detection of deceptive behavior in children whose data have been elicited through an ecologically valid paradigm.
A Comparison of a Moderate with Moderate-high Intensity Oral Anticoagulant Treatment in Patients with Mechanical Heart Valve Prostheses

Summary Background. The long-term administration of oral anticoagulants to patients with mechanical heart valve prostheses is generally accepted. However, the appropriate intensity of oral anticoagulant treatment in these patients is still controversial. Methods and Results. From March 1991 to March 1994, patients referred to the Padova Thrombosis Center who had undergone mechanical heart valve substitution at least 6 months earlier were randomly assigned to receive oral anticoagulants at moderate intensity (target INR = 3) or moderate-high intensity (target INR = 4). Principal end points were major bleeding, thromboembolism and vascular death. Minor bleeding was a secondary end-point. A total of 104 patients were assigned to the target 3 group and 101 to the target 4 group; they were followed for from 1.5 years to up 4.5 years (mean, 3 years). Principal end-points occurred in 13 patients in the target 3 group (4 per 100 patient-years) and in 20 patients in the target 4 group (6.9 per 100 patient-years). Major hemorrhagic events occurred in 15 patients, 4 in the target 3 group (1.2 per 100 patient-years) and 11 in the target 4 group (3.8 per 100 patient-years) (p = 0.019). The 12 recorded episodes of thromboembolism, 4 of which consisted of a visual deficit, were all transient ischemic attacks, 6 in the target 3 group (1.8 per 100 patient-years) and 6 in the target 4 group (2.1 per 100 patient- years). There were 3 vascular deaths in each group (0.9 and 1 per 100 patient-years for target 3 and target 4 groups, respectively). Minor bleeding episodes occurred 85 times (26 per 100 patient-years) in the target 3 group and 123 times (43 per 100 patient-years) in the target 4 group (p = 0.001). Conclusions. Mechanical heart valve patients on anticoagulant treatment who had been operated on at least 6 months earlier experienced fewer bleeding complications when maintained on a moderate intensity regimen (target INR = 3) than those on a moderate-high intensity regimen (target INR = 4). The number of thromboembolic events and vascular deaths did not differ between the two groups.