Miracle-dz/UniFAR-FTUs huggingface dataset:
The N-Terminal Domain of Chlorophyllide a Oxygenase Confers Protein Instability in Response to Chlorophyll b Accumulation in Arabidopsis

Plants acclimate to variations in light intensity by changing the antenna size of photosystems. This acclimation allows them to undergo efficient photosynthesis and creates a protective strategy to minimize photodamage. Chlorophyll b synthesis by chlorophyllide a oxygenase (CAO) is a key regulatory step in the control of antenna size. Recently, we found that higher plant CAOs consist of three domains (A, B, and C domains) and confirmed that the C domain possesses catalytic function. To investigate the function of the A domain, we fused various combinations of these three domains with green fluorescent protein (GFP) and introduced them into Arabidopsis thaliana. When a full-length CAO-GFP fusion protein was introduced into a chlorophyll b–less chlorina1-1 mutant, chlorophyll b accumulated to almost the same levels as in the chlorophyll b–containing Columbia wild type, but the CAO-GFP could not be detected by immunoblotting. By contrast, when a GFP-C domain fusion was introduced into chlorina1-1 or Columbia wild type, a large amount of GFP-C domain protein accumulated and the chlorophyll a/b ratio decreased drastically from 3.6 to 2.2 in Columbia wild type. When an A domain-GFP was introduced into Columbia wild type, A domain-GFP levels were very low. Conversely, a large amount of the protein accumulated when it was introduced into the chlorina1-1 mutant. These results indicate that the A domain may sense the presence of chlorophyll b and regulate the accumulation of CAO protein in the chloroplasts.
Fear and Courage: A Psychological Perspective

the population and the authorities greatly over-predicted how much fear would be provoked, especially during air raids on civilian populations. For example, in 1934 Winston Churchill warned the House of Commons to anticipate massive damage and casualties from repeated air attacks. He said the dangers were material but "no less formidable than these material effects are the reaction which will be produced upon the mind of the civilian population. We must expect . that at least 3 million or 4 million people will be driven out into the open country around the Metropolis" (Jenkins, 2002: 476). Contrary to the virtually universal expectation that repeated air attacks would produce mass panic and very large numbers of psychiatric casualties, people endured astonishingly well (Janis, 1951). The expected panic and uncontrollable fear did not occur.
A Search for Core Values: Towards a Model Code of Ethics for Information Professionals.

L'auteur examine differents codes ethiques promulgues par les associations professionnelles, relatifs aux pratiques des specialistes de l'information, en pointant les contradictions de ces systemes de valeurs entre eux : primaute des droits du client individuel contre primaute des droits de l'institution, du bien public ou de l'interet national ; role de l'organisme professionnel dans la reglementation du comportement des specialistes de l'information. Il cherche ainsi a mettre a jour le coeur des principes applicables aux professions de l'information et propose en conclusion un modele de code base sur ces principes.
Role of vascular smooth muscle cell in the inflammation of atherosclerosis

Atherosclerosis is a pathologic process occurring within the artery, in which many cell types, including T cell, macrophages, endothelial cells, and smooth muscle cells, interact, and cause chronic inflammation, in response to various inner- or outer-cellular stimuli. Atherosclerosis is characterized by a complex interaction of inflammation, lipid deposition, vascular smooth muscle cell proliferation, endothelial dysfunction, and extracellular matrix remodeling, which will result in the formation of an intimal plaque. Although the regulation and function of vascular smooth muscle cells are important in the progression of atherosclerosis, the roles of smooth muscle cells in regulating vascular inflammation are rarely focused upon, compared to those of endothelial cells or inflammatory cells. Therefore, in this review, we will discuss here how smooth muscle cells contribute or regulate the inflammatory reaction in the progression of atherosclerosis, especially in the context of the activation of various membrane receptors, and how they may regulate vascular inflammation. [BMB Reports 2014; 47(1): 1-7]
The “Weather Intelligence for Renewable Energies” Benchmarking Exercise on Short-Term Forecasting of Wind and Solar Power Generation

A benchmarking exercise was organized within the framework of the European Action Weather Intelligence for Renewable Energies (“WIRE”) with the purpose of evaluating the performance of state of the art models for short-term renewable energy forecasting. The exercise consisted in forecasting the power output of two wind farms and two photovoltaic power plants, in order to compare the merits of forecasts based on different modeling approaches and input data. It was thus possible to obtain a better knowledge of the state of the art in both wind and solar power forecasting, with an overview and comparison of the principal and the novel approaches that are used today in the field, and to assess the evolution of forecast performance with respect to previous benchmarking exercises. The outcome of this exercise consisted then in proposing new challenges in the renewable power forecasting field and identifying the main areas for improving accuracy in the future.
High-resolution imager for digital mammography: physical characterization of a prototype sensor

The physical performance characteristics of a high-resolution sensor module for digital mammography were investigated. The signal response of the imager was measured at various detector entrance air kerma and was found to be linear. The spatial resolution was determined by measuring the presampling modulation transfer function, MTF(f), of the system. The noise power spectra, NPS(f), of the system were estimated using 26 kVp: Mo/Mo, 28 kVp: Mo/Rh and 30 kVp: Rh/Rh, with polymethyl methacrylate (PMMA) ‘tissue equivalent material’ of thickness 20, 45 and 57 mm for each of three x-ray spectra at detector entrance air kerma in the range between ∼80.2 and 92.3 µGy. The noise equivalent quanta, NEQ(f), and detective quantum efficiencies, DQE(f), for the various spectral conditions were computed. In addition, dose dependence of NPS(f) and DQE(f) was studied at various detector entrance air kerma ranging from 9.4 to 169.7 µGy. A spatial resolution of about 10 cycles mm−1 was obtained at the 10% MTF(f) level. A small increase in NEQ(f)was observed under higher energy spectral conditions while the DQE(f) decreased marginally. For a given spectrum, increasing PMMA filtration produced negligible change in DQE(f). The estimated DQE values at zero frequency were in the range between 0.45 and 0.55 under the conditions investigated in this study.
Changing Teaching Takes More than a One-Shot Workshop.

The school reform movement is in trouble. In more than a century, no fundamental changes have been made in the way American teachers teach (Cuban 1990; Sarason 1971, 1983; Warren 1985). Further, student achievement is unchanged from 20 years ago, the Educational Testing Service recently concluded (Mullis et al. 1990). Perhaps most damning, ETS asserted that the rhetoric of instructional innovation far surpasses the reality of classroom change. Once again, it seems, reformers have underestimated the difficulty of achieving genuine changes in the ways teachers teach (Sarason 1971, 1990). This underestimation has occurred even in some otherwise commendable efforts, such as the new California curriculum frameworks (e.g., California State Department of Education 1987). These visionary frameworks are part of a more general movement toward active, constructive, goal-oriented learning by students (Shuell 1986, Putnam et al. 1990, Resnick and Klopfer 1989). If we are to achieve the goals of this new framework and similar efforts, important changes in teaching practices will be required. Unfortunately, however, if past experiences are any guide, these changes will elude the reformers. Everyone seems to want change, but with a few exceptions, the reform movement is not achieving its aims. What's the problem? Our research suggests that (1) the "new kinds of teaching" required to implement the reforms are described in terms too general for teachers to use, and (2) even if these new kinds of teaching were clearly defined, current staff develop ment practices are inadequate to effect meaningful changes. One solution, we suggest, is to say goodbye to quick-fix workshops. We must, instead, create contexts in teachers' work lives that assist and sustain meaningful changes. These contexts should consist, preeminently, of engaging teachers in rigorous exami nations of teaching: the concrete chal lenges and problems they face, the range of possible solutions, and, most important, close examination of whether, over time, there is progress in addressing these challenges. Staff development, in other words, must be grounded in the mundane but very real details of teachers' daily work lives and in a form that provides the
Collaboration in Education: The Phenomenon and Process of Working Together

In this article we propose a model for collaboration in education. We begin by examining the roots of collaboration in consultation, collegiality, and cooperation. We then delineate the features of collaboration as phenomenon and process, offering the view that through talk, action, and reflection among individuals a community of learners emerges. Finally, we suggest that collaboration can create a space that enables us to challenge taken-for-granted ways of working together and to bring about transformation in educational practice.
Foretinib is a potent inhibitor of oncogenic ROS1 fusion proteins

Significance ROS1 fusion kinases are critical oncogenes in several malignancies, suggesting that ROS1 inhibitors are likely to be effective molecularly targeted therapies in these patients. Although phase I/II clinical trials using the ALK/ROS1 inhibitor crizotinib to treat ROS1 fusion-harboring non–small-cell lung cancer patients demonstrate early success, evidence of clinical resistance to crizotinib due to the acquired ROS1G2032R mutation was recently reported. Here, we demonstrate that foretinib is a more potent ROS1 inhibitor than crizotinib in vitro and in vivo and remains effective against crizotinib-resistant ROS1 kinase domain mutations, including ROS1 G2032R. Taken together, our findings establish foretinib as a highly promising therapeutic candidate for treating patients with ROS1-driven malignancies and provide rationale for rapid clinical translation. The rapidly growing recognition of the role of oncogenic ROS1 fusion proteins in the malignant transformation of multiple cancers, including lung adenocarcinoma, cholangiocarcinoma, and glioblastoma, is driving efforts to develop effective ROS1 inhibitors for use as molecularly targeted therapy. Using a multidisciplinary approach involving small molecule screening in combination with in vitro and in vivo tumor models, we show that foretinib (GSK1363089) is a more potent ROS1 inhibitor than crizotinib (PF-02341066), an ALK/ROS inhibitor currently in clinical evaluation for lung cancer patients harboring ROS1 rearrangements. Whereas crizotinib has demonstrated promising early results in patients with ROS1-rearranged non–small-cell lung carcinoma, recently emerging clinical evidence suggests that patients may develop crizotinib resistance due to acquired point mutations in the kinase domain of ROS1, thus necessitating identification of additional potent ROS1 inhibitors for therapeutic intervention. We confirm that the ROS1G2032R mutant, recently reported in clinical resistance to crizotinib, retains foretinib sensitivity at concentrations below safe, clinically achievable levels. Furthermore, we use an accelerated mutagenesis screen to preemptively identify mutations in the ROS1 kinase domain that confer resistance to crizotinib and demonstrate that these mutants also remain foretinib sensitive. Taken together, our data strongly suggest that foretinib is a highly effective ROS1 inhibitor, and further clinical investigation to evaluate its potential therapeutic benefit for patients with ROS1-driven malignancies is warranted.
CRH and Lysine-Vasopressin Stimulation Tests in the Diagnosis of Hypoadrenalism Secondary to Hypothalamic or Pituitary Disorders

Ten patients with secondary hypoadrenalism have been tested with corticotropin releasing hormone (CRH) and lysine-vasopressin (LVP). One patient had isolated ACTH deficiency; 9 had deficiency of other pituitary hormones attributable to a primary pituitary disease in 3 and to an hypothalamic disorder in 6. After CRH administration, a definite increase in plasma ACTH was observed in all 6 patients with hypothalamic disorder. No response was elicited in the 3 patients with pituitary disease and in the patient with isolated ACTH deficiency. In the responsive patients. ACTH showed a delayed and prolonged pattern of response. Lysine-vasopressin administration produced an increase in plasma ACTH in 4 of the 6 hypothalamic patients and no response in those with pituitary disease and in the patient with isolated ACTH deficiency. These findings suggest that CRH represents a reliable test in differentiating hypothalamic from pituitary adrenal failure; LVP appeared a less sensitive diagnostic test.
Epithelial Cell Differentiation Pathways in the Human Prostate: Identification of Intermediate Phenotypes by Keratin Expression

SUMMARY The prostate grows slowly throughout adult life, leading to benign prostatic hyperplasia (BPH), which often results in urethral obstruction in later years. The symptoms of BPH are the second most common reason for surgery in men over 65. The aim of this study was to determine the relationship between cell proliferation and cell differentiation in BPH tissue. Using multiple antibodies, simultaneously detected with different fluorophore-conjugated secondary antibodies, several subpopulations of epithelial cells were detected. In addition to K14, basal cells also expressed keratins 15, 17, and 19 in various combinations, and some of the luminal cells also expressed K19 together with K8 and K18. Co-staining for cytokeratins and Ki-67 indicated that 44% of proliferative cells expressed K14 and 36% K19, although the difference was not statistically significant. This report provides a detailed description of the relationship between keratin expression and cell proliferation in the prostate and indicates that K19-positive cells form the link between the basal and luminal layers of the epithelium. (J Histochem Cytochem 49:271–278, 2001)
Power Scaling of Uplink Massive MIMO Systems With Arbitrary-Rank Channel Means

This paper investigates the uplink achievable rates of massive multiple-input multiple-output (MIMO) antenna systems in Ricean fading channels, using maximal-ratio combining (MRC) and zero-forcing (ZF) receivers, assuming perfect and imperfect channel state information (CSI). In contrast to previous relevant works, the fast fading MIMO channel matrix is assumed to have an arbitrary-rank deterministic component as well as a Rayleigh-distributed random component. We derive tractable expressions for the achievable uplink rate in the large-antenna limit, along with approximating results that hold for any finite number of antennas. Based on these analytical results, we obtain the scaling law that the users' transmit power should satisfy, while maintaining a desirable quality of service. In particular, it is found that regardless of the Ricean K-factor, in the case of perfect CSI, the approximations converge to the same constant value as the exact results, as the number of base station antennas, M, grows large, while the transmit power of each user can be scaled down proportionally to 1/M. If CSI is estimated with uncertainty, the same result holds true but only when the Ricean K-factor is non-zero. Otherwise, if the channel experiences Rayleigh fading, we can only cut the transmit power of each user proportionally to 1/√M. In addition, we show that with an increasing Ricean K-factor, the uplink rates will converge to fixed values for both MRC and ZF receivers.
Simultaneous routing and resource allocation via dual decomposition

In wireless data networks, the optimal routing of data depends on the link capacities which, in turn, are determined by the allocation of communications resources (such as transmit powers and bandwidths) to the links. The optimal performance of the network can only be achieved by simultaneous optimization of routing and resource allocation. In this paper, we formulate the simultaneous routing and resource allocation (SRRA) problem, and exploit problem structure to derive efficient solution methods. We use a capacitated multicommodity flow model to describe the data flows in the network. We assume that the capacity of a wireless link is a concave and increasing function of the communications resources allocated to the link, and the communications resources for groups of links are limited. These assumptions allow us to formulate the SRRA problem as a convex optimization problem over the network flow variables and the communications variables. These two sets of variables are coupled only through the link capacity constraints. We exploit this separable structure by dual decomposition. The resulting solution method attains the optimal coordination of data routing in the network layer and resource allocation in the radio control layer via pricing on the link capacities.
Differential Gene Expression Patterns of EBV Infected EBNA-3A Positive and Negative Human B Lymphocytes

The genome of Epstein-Barr virus (EBV) encodes 86 proteins, but only a limited set is expressed in EBV–growth transformed B cells, termed lymphoblastoid cell lines (LCLs). These cells proliferate via the concerted action of EBV nuclear antigens (EBNAs) and latent membrane proteins (LMPs), some of which are rate limiting to establish a stable homeostasis of growth promoting and anti-apoptotic activities. We show here that EBV mutants, which lack the EBNA-3A gene, are impaired but can still initiate cell cycle entry and proliferation of primary human B cells in contrast to an EBNA-2 deficient mutant virus. Surprisingly, and in contrast to previous reports, these viral mutants are attenuated in growth transformation assays but give rise to permanently growing EBNA-3A negative B cell lines which exhibit reduced proliferation rates and elevated levels of apoptosis. Expression profiles of EBNA-3A deficient LCLs are characterized by 129 down-regulated and 167 up-regulated genes, which are significantly enriched for genes involved in apoptotic processes or cell cycle progression like the tumor suppressor gene p16/INK4A, or might contribute to essential steps of the viral life cycle in the infected host. In addition, EBNA-3A cellular target genes remarkably overlap with previously identified targets of EBNA-2. This study comprises the first genome wide expression profiles of EBNA-3A target genes generated within the complex network of viral proteins of the growth transformed B cell and permits a more detailed understanding of EBNA-3A's function and contribution to viral pathogenesis.
EVALUATING ONLINE DISCUSSIONS: FOUR DIFFERENT FRAMES OF ANALYSIS

This study uses four different “frames” to analyze 17 online discussions that occurred in two doctoral level classes in educational leadership. Two of the frames were developmental models: King andKitchener’s Reflective Judgment Model and Perry’s model of intellectual and ethical development. Two of the frames captured levels of thinking: Garrison’s four-stage critical-thinking model and Bloom’s taxonomy of educational objectives. Of the 278 individual postings, 45.3% were at levels five through seven of the King and Kitchener model, 100% were at levels five through nine of the Perry model, 52.2% were at the two highest levels of the Garrison model, and 54.3% were at levels four through six in Bloom’s taxonomy. These results seem appropriate to the level of response expected of doctoral students. For each frame, the analysis resulted in additional findings. The study concludes that each frame has value and focuses attention on different aspects of the student’s thinking as evidenced in his/her posting to an online discussion; however, some frames are more difficult to use than others, which argues for specific training and/or tailoring the topic of discussions to address issues in a particular manner. Lastly, the question initiating each of the online discussions influenced the level of the responses from students. Each frame has the potential to illumine students’ online discussions, although using multiple frames may have more benefit than using any one frame exclusively.
Counterfactual thinking and emotions: regret and envy learning

Emotions like regret and envy share a common origin: they are motivated by the counterfactual thinking of what would have happened had we made a different choice. When we contemplate the outcome of a choice we made, we may use the information on the outcome of a choice we did not make. Regret is the purely private comparison between two choices that we could have taken, envy adds to this the information on outcome of choices of others. However, envy has a distinct social component, in that it adds the change in the social ranking that follows a difference in the outcomes. We study the theoretical foundation and the experimental test of this view.
Metastatic chondroblastoma. Report of a fatal case with a review of the literature on atypical, aggressive, and malignant chondroblastoma

A boy with metastatic and fatal chondroblastoma is presented. Unlike previously published examples of metastatic chondroblastoma, these metastases developed before any operative manipulation of the primary tumor. The histologic characteristics of the primary, metastatic, and locally recurrent tumors were those of a conventional chondroblastoma. A review of published cases of atypical, aggressive, and malignant chondroblastoma is presented with current follow‐up information. Although some metastatic chondroblastomas may result from operative manipulation of the primary tumor and are clinically benign, other histologically benign chondroblastomas exist that are capable of pursuing a malignant course. The authors designate these as malignant chondroblastomas. No histologic criteria exist for the separation of these tumors.
Integration of multiple and asynchronous acoustic cues to word initial fricatives and context compensation in 7-year-olds, 12-year-olds and adults

ion) from frication alone because listeners would not even recognize the frication as speech (without additional information). However, an abstracted buffer is compatible with the view that frication is buffered because it is not temporal in nature like VOT, and the possibility that listeners do not integrate frication until they are able to compensate for context. This is because while listeners must wait until the offset of frication or the availability of context to utilize acoustic cues, they at least recognize frication as part of the speech stream, and therefore would be capable of extracting cue values. The first step towards teasing apart these two possibilities is a reanalysis of the present eye-tracking data. In the analyses presented here frication was treated as an