Miracle-dz/UniFAR-FTUs huggingface dataset:
Using Perls Staining to Trace the Iron Uptake Pathway in Leaves of a Prunus Rootstock Treated with Iron Foliar Fertilizers

The aim of this study was to trace the Fe uptake pathway in leaves of Prunus rootstock (GF 677; Prunus dulcis × Prunus persica) plants treated with foliar Fe compounds using the Perls blue method, which detects labile Fe pools. Young expanded leaves of Fe-deficient plants grown in nutrient solution were treated with Fe-compounds using a brush. Iron compounds used were the ferrous salt FeSO4, the ferric salts Fe2(SO4)3 and FeCl3, and the chelate Fe(III)-EDTA, all of them at concentrations of 9 mM Fe. Leaf Fe concentration increases were measured at 30, 60, 90 min, and 24 h, and 70 μm-thick leaf transversal sections were obtained with a vibrating microtome and stained with Perls blue. In vitro results show that the Perls blue method is a good tool to trace the Fe uptake pathway in leaves when using Fe salts, but is not sensitive enough when using synthetic Fe(III)-chelates such as Fe(III)-EDTA and Fe(III)-IDHA. Foliar Fe fertilization increased leaf Fe concentrations with all Fe compounds used, with inorganic Fe salts causing larger leaf Fe concentration increases than Fe(III)-EDTA. Results show that Perls blue stain appeared within 30 min in the stomatal areas, indicating that Fe applied as inorganic salts was taken up rapidly via stomata. In the case of using FeSO4 a progression of the stain was seen with time toward vascular areas in the leaf blade and the central vein, whereas in the case of Fe(III) salts the stain mainly remained in the stomatal areas. Perls stain was never observed in the mesophyll areas, possibly due to the low concentration of labile Fe pools.
The tackle in South African youth rugby union – Gap between coaches' knowledge and training behaviour

In youth rugby union matches, tackle-related injuries account for 60% of all injuries, 62% of concussion injuries and almost 50% of spinal cord injuries (youth and amateur). Because of this high risk of injury, the inclusion of the tackle in youth rugby has been a topic of discussion in the public and a high priority research area for World Rugby. What a coach knows and his/her attitude toward player safety directly impacts the risk and performance profile of a player. The purpose of this study is to describe the tackle knowledge, attitudes and training behaviours of youth rugby coaches. The entire population of Western Province Rugby Union Premier A1 division (highest level of school rugby) under-19 rugby coaches (n = 8) completed a knowledge and attitude questionnaire and 96 field-training sessions were observed over four weeks. Coaches rated tackling (mean 3.9, 95% confidence interval 3.3–4.4), rucking (mean 3.8, 95% confidence interval 3.0–4.5) and ball-carrying (mean 3.6, 95% confidence interval 2.6–4.6) as high-risk of injury facets of play (H = 30.8, p < 0.001). Coaching proper technique was rated as very important for safety (mean 4.6, 95% confidence interval 4.2–5.0) and performance (mean 4.8, 95% confidence interval 4.4–5.0, U = 28, p > 0.05). Of the 96 observed training sessions, tackle training was recorded 16% of the time (vs. 84% no tackle training, p < 0.001). Coaches were aware of the risk of injury in the tackle and rated the coaching of proper technique of utmost importance. These positive knowledge and attitudes did not transfer into their tackle training. The discrepancy between coaches' tackle knowledge and attitudes, and their training of the tackle might be related to how competent they believe themselves to be in delivering tackle training.
Cultural Mediators Seduced by Mad Men: How Cultural Journalists Legitimized a Quality TV Series in the Nordic Region

Based on theories about the role of cultural mediators in cultural production and using the TV series Mad Men as a case, this article investigates how cultural journalists in the Nordic countries have contributed to legitimizing “quality TV series” as a worthy field of aesthetic consumption. Key analytical points are as follows: (1) cultural journalists legitimize Mad Men’s quality by addressing aspects internal (aesthetic markers) and aspects external (culture industry markers) to the series, as well as the series’ broader social and historical anchoring; (2) Nordic cultural journalists position themselves positively toward the TV series based on their professional expertise and their personal taste preferences and predilections; (3) these legitimation processes take place across journalistic genres, pointing to the importance not only of TV criticism, epitomized by the review, but of cultural journalism more broadly in constructing affirmative attitudes toward popular culture phenomena such as TV series.
TextLogger: inferring longer inputs on touch screen using motion sensors

Today's smartphones are equipped with precise motion sensors like accelerometer and gyroscope, which can measure tiny motion and rotation of devices. While they make mobile applications more functional, they also bring risks of leaking users' privacy. Researchers have found that tap locations on screen can be roughly inferred from motion data of the device. They mostly utilized this side-channel for inferring short input like PIN numbers and passwords, with repeated attempts to boost accuracy. In this work, we study further for longer input inference, such as chat record and e-mail content, anything a user ever typed on a soft keyboard. Since people increasingly rely on smartphones for daily activities, their inputs directly or indirectly expose privacy about them. Thus, it is a serious threat if their input text is leaked. To make our attack practical, we utilize the shared memory side-channel for detecting window events and tap events of a soft keyboard. The up or down state of the keyboard helps triggering our Trojan service for collecting accelerometer and gyroscope data. Machine learning algorithms are used to roughly predict the input text from the raw data and language models are used to further correct the wrong predictions. We performed experiments on two real-life scenarios, which were writing emails and posting Twitter messages, both through mobile clients. Based on the experiments, we show the feasibility of inferring long user inputs to readable sentences from motion sensor data. By applying text mining technology on the inferred text, more sensitive information about the device owners can be exposed.
SGLT2 inhibitor lowers serum uric acid through alteration of uric acid transport activity in renal tubule by increased glycosuria

Sodium glucose cotransporter 2 (SGLT2) inhibitors have been reported to lower the serum uric acid (SUA) level. To elucidate the mechanism responsible for this reduction, SUA and the urinary excretion rate of uric acid (UEUA) were analysed after the oral administration of luseogliflozin, a SGLT2 inhibitor, to healthy subjects. After dosing, SUA decreased, and a negative correlation was observed between the SUA level and the UEUA, suggesting that SUA decreased as a result of the increase in the UEUA. The increase in UEUA was correlated with an increase in urinary d‐glucose excretion, but not with the plasma luseogliflozin concentration. Additionally, in vitro transport experiments showed that luseogliflozin had no direct effect on the transporters involved in renal UA reabsorption. To explain that the increase in UEUA is likely due to glycosuria, the study focused on the facilitative glucose transporter 9 isoform 2 (GLUT9ΔN, SLC2A9b), which is expressed at the apical membrane of the kidney tubular cells and transports both UA and d‐glucose. It was observed that the efflux of [14C]UA in Xenopus oocytes expressing the GLUT9 isoform 2 was trans‐stimulated by 10 mm d‐glucose, a high concentration of glucose that existed under SGLT2 inhibition. On the other hand, the uptake of [14C]UA by oocytes was cis‐inhibited by 100 mm d‐glucose, a concentration assumed to exist in collecting ducts. In conclusion, it was demonstrated that the UEUA could potentially be increased by luseogliflozin‐induced glycosuria, with alterations of UA transport activity because of urinary glucose. © 2014 The Authors. Biopharmaceutics & Drug Disposition. Published by John Wiley & Sons Ltd.
Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts

Baby hamster kidney (BHK) fibroblasts increase their cell capacitance by 25–100% within 5 s upon activating maximal Ca influx via constitutively expressed cardiac Na/Ca exchangers (NCX1). Free Ca, measured with fluo-5N, transiently exceeds 0.2 mM with total Ca influx amounting to ∼5 mmol/liter cell volume. Capacitance responses are half-maximal when NCX1 promotes a free cytoplasmic Ca of 0.12 mM (Hill coefficient ≈ 2). Capacitance can return to baseline in 1–3 min, and responses can be repeated several times. The membrane tracer, FM 4-64, is taken up during recovery and can be released at a subsequent Ca influx episode. Given recent interest in signaling lipids in membrane fusion, we used green fluorescent protein (GFP) fusions with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and diacylglycerol (DAG) binding domains to analyze phospholipid changes in relation to these responses. PI(4,5)P2 is rapidly cleaved upon activating Ca influx and recovers within 2 min. However, PI(4,5)P2 depletion by activation of overexpressed hM1 muscarinic receptors causes only little membrane fusion, and subsequent fusion in response to Ca influx remains massive. Two results suggest that DAG may be generated from sources other than PI(4,5)P in these protocols. First, acylglycerols are generated in response to elevated Ca, even when PI(4,5)P2 is metabolically depleted. Second, DAG-binding C1A-GFP domains, which are brought to the cell surface by exogenous ligands, translocate rapidly back to the cytoplasm in response to Ca influx. Nevertheless, inhibitors of PLCs and cPLA2, PI(4,5)P2-binding peptides, and PLD modification by butanol do not block membrane fusion. The cationic agents, FM 4-64 and heptalysine, bind profusely to the extracellular cell surface during membrane fusion. While this binding might reflect phosphatidylserine (PS) “scrambling” between monolayers, it is unaffected by a PS-binding protein, lactadherin, and by polylysine from the cytoplasmic side. Furthermore, the PS indicator, annexin-V, binds only slowly after fusion. Therefore, we suggest that the luminal surfaces of membrane vesicles that fuse to the plasmalemma may be rather anionic. In summary, our results provide no support for any regulatory or modulatory role of phospholipids in Ca-induced membrane fusion in fibroblasts.
People With Cerebral Palsy: Effects of and Perspectives for Therapy

The movement disorder of cerebral palsy (CP) is expressed in a variety of ways and to varying degrees in each individual. The condition has become more complex over the last 20 years with the increasing survival of children born at less than 28 to 30 weeks gestationai age. Impairments present in children with CP as a direct result of the brain injury or occurring indirectly to compensate for underlying problems include abnormal muscle tone; weakness and lack of fitness; limited variety of muscle synergies; contracture and altered biomechanics, the net result being limited functional ability. Other contributors to the motor disorder include sensory, cognitive and perceptual impairments. In recent years understanding of the motor problem has increased, but less is known about effects of therapy. Evidence suggests that therapy can improve functional possibilities for children with cerebral palsy but is inconclusive as to which approach might be most beneficial. The therapist requires an understanding of the interaction of all systems, cognitive/perceptual, motor, musculoskeletal, sensory and behavioral, in the context of the development and plasticity of the CNS. It is necessary to understand the limitations of the damaged immature nervous system, but important to optimize the child's functional possibilities.
Additive Effects of Genetic Variation in Dopamine Regulating Genes on Working Memory Cortical Activity in Human Brain

Functional polymorphisms in the catechol-O-methyltransferase (COMT) and the dopamine transporter (DAT) genes modulate dopamine inactivation, which is crucial for determining neuronal signal-to-noise ratios in prefrontal cortex during working memory. We show that the COMT Met158 allele and the DAT 3′ variable number of tandem repeat 10-repeat allele are independently associated in healthy humans with more focused neuronal activity (as measured with blood oxygen level-dependent functional magnetic resonance imaging) in the working memory cortical network, including the prefrontal cortex. Moreover, subjects homozygous for the COMT Met allele and the DAT 10-repeat allele have the most focused response, whereas the COMT Val and the DAT 9-repeat alleles have the least. These results demonstrate additive genetic effects of genes regulating dopamine signaling on specific neuronal networks subserving working memory.
Binary output of cellular neural networks with smooth activation

An important property of cellular neural networks (CNN's) is the binary output property, that, when the self-feedback is greater than one, the final activations are /spl plusmn/1. This brief considers the generalization of this property to networks with sigmoidal output functions. It is shown that in this case the property cannot be stated without reference to the cross feedback, and conditions are found under which the property remains valid.
The Antinociceptive Effect of Milnacipran in the Monosodium Iodoacetate Model of Osteoarthritis Pain and Its Relation to Changes in Descending Inhibition

Osteoarthritis (OA) is a chronic joint disorder whose principal symptom is chronic pain. Current analgesics are inadequate and the mechanisms contributing to this pain are poorly understood but likely to include both local joint changes and central consequences. These studies used monoamine receptor agents combined with behavioral studies and single-unit dorsal horn recordings to examine whether descending noradrenergic and serotonergic inhibitions are altered in the monosodium iodoacetate model of OA pain, and whether increasing these inhibitions with the serotonin/noradrenaline reuptake inhibitor milnacipran can attenuate the attendant hypersensitivity. Early and late in the course of this model, milnacipran (s.c.) reduced behavioral hypersensitivity, and inhibited evoked responses from sensitized dorsal horn neurons. In naïve animals and the early, but not late, phase of the model, spinal administration of the α2-adrenoceptor antagonist atipamezole fully reversed this neuronal inhibition, whereas atipamezole administered alone revealed that endogenous noradrenergic inhibition was reduced in the late phase. Blocking spinal 5-hydroxytryptamine-7 receptors with (2R)-1-[(3-hydroxyphenyl)sulfonyl]-2-[2-(4-methyl-1-piperidinyl)ethyl]pyrrolidine hydrochloride suggested that the effects of milnacipran in the late phase were partly mediated by these receptors, and that descending serotonergic inhibition was increased in this phase. An opioidergic mechanism behind the effects of milnacipran was indicated by a partial reversal of these effects with naloxone. These studies demonstrate antinociceptive effects for milnacipran in a model of OA pain, whose effects come via descending serotonergic and noradrenergic, as well as opioidergic, pathways. Variations in the activity of these pathways over the course of this model may contribute to the presence of behavioral hypersensitivity and determine through which endogenous systems milnacipran exerts its effects.
The Implementation of Inquiry Based Learning toward Students' Learning Outcomes and Critical Thinking Skills

This research aimed to (1) test the effect of inquiry based learning toward learning outcome of cognitive product and cognitive process, (2) test the effect of inquiry-based learning toward critical thinking skills. Implementation this model using the quasi-experimental. The independent variable was inquirybased learning while the dependent variables were learning outcome of cognitive product, cognitive process, and critical thinking skills. This research population was grade 11th students from SMA Negeri 1 Sungai Tabuk i.e. XI IPA-1, XI IPA-2, and XI IPA-3. This research samples for implementation of inquirybased learning toward learning outcomes for the first treatment classes were XI IPA-2 and XI IPA-3, while the control class was XI IPA-1. The second treatment classes were XI IPA-1 and XI IPA-2, while the control class was XI IPA-3. The treatment classes were determined by using purposive sampling technique. This research samples for implementation of inquiry based learning toward critical thinking skills were XI IPA-2 and XI IPA-3, while the control class was XI IPA-1. This research was held for 3 months. The instrument to measure the cognitive product and cognitive process consisted of multiple-choice test and the critical thinking skills consisted of essay test. The data were analyzed by using anacova SAS release 9.1. 3. The results are (1) inquiry base learning has effect to learning outcome of cognitive product and cognitive process, (2) inquiry base learning has no effect to the ability to apply, but has effect to the ability to analyze and the ability to evaluate. Keywords—Critical Thinking Skills, Inquiry Model, Learning Outcome
Managing Patients With Psoriasis in the Busy Clinic

Psoriasis is a common inflammatory disease with significant comorbidities, whose management can be challenging given the variety of treatment options. It is critical for nurse practitioners, physician assistants, general practitioners, and dermatology trainees to have useful information about the treatment and monitoring of patients with psoriasis. Although certain aspects of care apply to all patients, each therapeutic agent has its own nuances in terms of assessments, dosing, and monitoring. The most appropriate treatment is based not only on disease severity but also on comorbid conditions and concomitant medications. These practitioners are vital in facilitating patient care by thorough understanding of systemic agents, selection criteria, dosing, and recommended monitoring. This article provides high-yield practical pearls on managing patients with moderate to severe psoriasis. It includes case-based discussions illustrating considerations for special populations, such as pregnant women, children, and patients with comorbidities (eg, human immunodeficiency virus infection, hepatitis C, hepatitis B, and history of malignancy).
Creative Arts Therapies Students' Professional Development: Mixed Methods Longitudinal Research

This mixed-methods longitudinal study sought to describe, understand, and explain the professional development (PD) of graduate students during art therapy (AT), dance movement therapy (DMT), and drama therapy (DT) training (N = 51). Repeated measures of students’ scores in profession-related variables were performed. Quantitative theory testing probed the extent to which students confirm Rønnestad and Skovholt’s (2003) theory of counselors and therapists’ PD as representative of their pre-training and in-training experiences. Qualitative theory derivation generated data-driven concepts to modify and refine the extant theory. Data were collected using My Vocational Situation, Career Commitment Measurement, and questionnaires for each developmental phase. Data analysis involved descriptive statistics, analyses of variance, correlation analyses, repeated measures, qualitative data quantification, measurements of inter-rater agreement, and thematic analysis. Results indicate that whereas students’ professional identity and career commitment significantly increased, this increase did not differ between the three modality specializations; yet, scores of DMT and next AT students were overall significantly higher than scores of DT students. Students’ perceived environmental and personal barriers to career decision-making decreased; yet perceived financial barriers slightly increased. Results also revealed that students entered training for various motivations. The results confirm that transition into training provoked stress and anxiety for all students and that most students avoided art making outside of training. In-training arts making, exposure to different modalities, and professional socialization in the field cultivated students’ PI and sense of belonging to the CAT profession. A flow chart displays the emerged model of students’ PD process, policy and practice implications are suggested, and recommendations for further research are offered.
Physiotherapy for people with movement disorders arising from basal ganglia dysfunction.

Two of the most common diseases of the basal ganglia are Huntington’s disease (HD) and Parkinson’s disease (PD). In this article we compare and contrast the aetiology, pathogenesis and movement disorders associated with HD and PD, as a basis for deriving a framework for physiotherapy practice for people with basal ganglia dysfunction. Although much has been written about therapy for PD, little is known about the effects of allied health interventions on outcomes in HD. It is possible that strategies used to optimise movement in PD could also be beneficial for select movement disorders in HD because PD and HD share some common pathological features and symptoms. Movement disorders common to HD and PD include hypokinesia, akinesia and dyskinesia. Secondary musculoskeletal and cardiovascular changes can also occur as a result of inactivity or disuse. A framework for physiotherapy practice for people with basal ganglia disorders is proposed, based upon scientific evidence from the movement disorders literature. For both conditions, the major aim is to teach people how to by-pass the defective basal ganglia structures and to use frontal neural pathways to control movement. Belinda Bilney*, Meg E Morris, Sonia Denisenko. Physiotherapy for people with movement disorders arising from basal ganglia dysfunction. New Zealand Journal of Physiotherapy 31(2): 94-100.
Accumulation of F-Spondin in Injured Peripheral Nerve Promotes the Outgrowth of Sensory Axons

F-spondin, an extracellular matrix protein, is present in peripheral nerve during embryonic development, but its amount diminishes by birth. Axotomy of adult rat sciatic nerve, however, causes a massive upregulation of both F-spondin mRNA and protein distal to the lesion. F-spondin in the distal stump of axotomized nerve promotes neurite outgrowth of sensory neurons, as revealed by protein neutralization with F-spondin-specific antibodies. Thus, F-spondin is likely to play a role in promoting axonal regeneration after nerve injury.