Miracle-dz/UniFAR-FTUs huggingface dataset:
Foxp4 Is Dispensable for T Cell Development, but Required for Robust Recall Responses

Transcription factors regulate T cell fates at every stage of development and differentiation. Members of the Foxp family of forkhead transcription factors are essential for normal T lineage development; Foxp3 is required for T regulatory cell generation and function, and Foxp1 is necessary for generation and maintenance of naïve T cells. Foxp4, an additional member of the Foxp family, is highly homologous to Foxp1 and has been shown to dimerize with other Foxp proteins. We report the initial characterization of Foxp4 in T lymphocytes. Foxp4 is expressed in both thymocytes and peripheral CD4+ and CD8+ T cells. We used a CD4Cre mediated approach to evaluate the cell autonomous role for Foxp4 in murine T lymphocytes. T cell development, peripheral cellularity and cell surface phenotype are normal in the absence of Foxp4. Furthermore, Foxp3+ T regulatory cells develop normally in Foxp4 deficient animals and naïve Foxp4 deficient CD4 T cells can differentiate to inducible T regulatory cells in vitro. In wild-type T cells, expression of Foxp4 increases following activation, but deletion of Foxp4 does not affect T cell proliferative responses or in vitro effector T cell differentiation. In vivo, despite effective control of Toxoplasma gondii and acute lymphocytic choriomeningitis virus infections, effector cytokine production during antigen specific recall responses are reduced in the absence of Foxp4. We conclude that Foxp4 is dispensable for T cell development, but necessary for normal T cell cytokine recall responses to antigen following pathogenic infection.
CRESTBOT: A New Family of Resilient Botnets

We show that it is possible to design botnet structures called CRESTBOT based on extractor graphs which are highly resilient to command-and-control (C&C) take-downs, yet do not require significant changes to existing botnet designs and codes, and do not suffer from the implementation complexity of P2P-based and hybrid structures. The UDP family of CRESTBOT is shown to be able to send commands from the botmaster much faster than traditional botnet. Our analyses are validated by extensive experiments on Emulab. Our results prove that current C&C-takedown solutions are ineffective against well designed botnets such as our crestbot. Secondly, short UDP commands can be as reliable as TCP commands with much less time consumption. Third, extremely fast command issuing is possible, which at first glance might seem beneficial to the attacker; however, it might also be of use for the "good guys" when certain race conditions are desired such as software patching or quick bot takedowns.
A genealogical assessment of heritable predisposition to aneurysms.

OBJECT This study was conducted to investigate the familial and genetic contribution to intracranial, abdominal aortic, and all other types of aneurysms, and to define familial relationships among patients who present with the different aneurysm types. METHODS The authors used a unique Utah resource to perform population-based analysis of the familial nature of aneurysms. The Utah Population Data Base is a genealogy of the Utah population dating back eight generations, which is combined with death certificate data for the state of Utah dating back to 1904. Taking into account the genetic relationships among all aneurysm cases derived from this resource, the authors used a previously published method to estimate the familiality of different aneurysm types. Using internal, birth-cohort-specific rates of disease calculated from the database, they estimated relative risks by comparing observed to expected rates of aneurysm incidence in defined sets of relatives of probands. CONCLUSIONS Each of the three aneurysm types investigated showed significant evidence for a genetic component. Relatives of patients with intracranial aneurysms do not appear to be at increased risk for abdominal or other lesions, but relatives of patients with abdominal aortic aneurysms appear to be at increased risk for other types of these lesions.
Effects of Cognitive Load and Anticipation on Driver Responses to a Critical Traffic Event

Existing studies on the effects of cognitive load on drivers’ responses to critical traffic events have yielded inconsistent results. The recently proposed cognitive control hypothesis offers a potential explanation for this discrepancy by suggesting that cognitive load selectively impairs aspects of driving relying on cognitive control, that is, novel, non-practiced or inherently difficult tasks, but leaves automatized responses unaffected. The present driving simulator study addressed one specific prediction of the cognitive control hypothesis, namely that the effect of cognitive load on responses to a traffic event should depend on whether the event it anticipated. The results generally supported this prediction, showing no effects of cognitive load on braking and gaze responses to an initial non-anticipated event and gradually increasing cognitive load effects as the scenario was repeated.
Pacific Asia Conference on Information Systems ( PACIS ) 6-18-2013 Down-to-Earth Issues in Information System Use

This study proposes a new understanding of IS use that gives attention to the everyday 'Down-ToEarth' (DTE) issues that actually affect the success or quality of IS use. These differ from the kinds of issues discussed in the extant literature on IS use, in at least five main ways. The DTE approach takes the user's everyday perspective rather than that of management, IT suppliers or researchers. It widens the often narrow focus on single aspects of IS use. It recognises the importance of indirect and hidden issues, which are often overlooked. And it provides a way of coping with the plethora of issues that result from this. To achieve this, the DTE approach makes use of the suite of fifteen aspects that emerge from Dooyeweerd's philosophy of everyday life. Empirical research has been undertaken to operationalize the DTE approach as a qualitative analysis method enriched with Dooyeweerd's aspects. This showed that the kinds of issues found by the DTE approach are not only more numerous than with conventional approaches, but also of different kinds, giving a richer and wider picture of IS use. Contributions to theory, methodology and practice are briefly discussed
Orienting Response and Memory for Web Advertisements:

This study investigated the effects of pop-up windows and animation on online users’ orienting response and memory for Web advertisements. All participants (N = 60) in a mixed-design factorial experiment were exposed to four online portal Web sites, each containing a banner ad that was either animated or static and a pop-up ad that was also either animated or static. Their orienting responses during reception of the online sites were measured via heartbeats using electrocardiogram (ECG). Recall and recognition memory for ads and portal Web sites were measured via a postexposure paper-and-pencil questionnaire. Hypotheses derived from visual attention, motion effect, distinctiveness, bio-informational, and limited-capacity theories were tested. The results fully supported the proposition that pop-up ads elicit orienting responses. Ad recognition was lower whereas ad recall was higher for pop-up ads compared to banner ads. In addition to main effects, the data revealed several interaction effects, with implications for theory.
Critical thinking: A voyage of the imagination

In this dissertation I contend that there is a strong connection between critical thinking and the imagination, a connection which increases the dynamism and vitality of critical thinking. By acknowledging a role for the imagination, we are able to form a more coherent and complete critical thinking conception, which leads to the positing of a new theory of critical thinking. This new conception has pedagogical implications demanding that we alter or augment current approaches to critical thinking instruction. Employing a conceptual analysis, I first focus on critical thinking conceptions found on a continuum from traditional conceptions, which focus on logic and argument analysis, to expanded conceptions, which are more eclectic and admit a role for the affective as well as the cognitive. In order to focus on the nature of the imagination, which I argue plays an important role especially in expanded conceptions of critical thinking, I examine first the philosophical and then the literary conceptions of the imagination, specifically considering the arguments by the philosophers Edward Casey and Mary Warnock and the writers William Wordsworth, Samuel Taylor Coleridge, and Wallace Stevens. These philosophers and writers reveal an imagination characterized by a connection to creativity, the capacity to construct meaning, the generation of potentially unending possibilities, the capacity to enable the emotions to emerge and coexist with rationality. Other writers and literary theorists like Samuel Johnson, Toni Morrison, and Deanne Bogdan alert us to the epistemological and moral dangers of the imagination, dangers which need to be acknowledged and addressed in order to allow for the imagination to fully enrich and enhance critical thinking. The new conception of critical thinking, which I call integrative critical thinking, fully employs the imagination to generate a variety of possible avenues for our thinking and our conclusions, evokes emotions held in creative tension with reason, envisages a conclusion (or conclusions) to one's thought process and the means to reach those conclusions, and allows for creativity during the critical thinking process. Integrative critical thinking incorporates criticism and judgment, but also recognizes that critical thinking occurs in and is affected by a social context. This conception integrates the three enduring approaches (knowledge, skills, and dispositions) to critical thinking and opens up the critical thinker not only to envision a liberated state of mind and being but also to act on that vision.
Functional Expression and Characterization of Schizosaccharomyces pombe Avt3p as a Vacuolar Amino Acid Exporter in Saccharomyces cerevisiae

In Saccharomyces cerevisiae, Avt3p and Avt4p mediate the extrusion of several amino acids from the vacuolar lumen into the cytosol. SpAvt3p of Schizosaccharomyces pombe, a homologue of these vacuolar amino acid transporters, has been indicated to be involved in spore formation. In this study, we confirmed that GFP-SpAvt3p localized to the vacuolar membrane in S. pombe. The amounts of various amino acids increased significantly in the vacuolar pool of avt3Δ cells, but decreased in that of avt3 +-overexpressing avt3Δ cells. These results suggest that SpAvt3p participates in the vacuolar compartmentalization of amino acids in S. pombe. To examine the export activity of SpAvt3p, we expressed the avt3 + gene in S. cerevisiae cells. We found that the heterologously overproduced GFP-SpAvt3p localized to the vacuolar membrane in S. cerevisiae. Using the vacuolar membrane vesicles isolated from avt3 +-overexpressing S. cerevisiae cells, we detected the export activities of alanine and tyrosine in an ATP-dependent manner. These activities were inhibited by the addition of a V-ATPase inhibitor, concanamycin A, thereby suggesting that the activity of SpAvt3p is dependent on a proton electrochemical gradient generated by the action of V-ATPase. In addition, the amounts of various amino acids in the vacuolar pools of S. cerevisiae cells were decreased by the overproduction of SpAvt3p, which indicated that SpAvt3p was functional in S. cerevisiae cells. Thus, SpAvt3p is a vacuolar transporter that is involved in the export of amino acids from S. pombe vacuoles.
Program on Education Policy and Governance Working Papers Series the Educational Consequences of Blinding Weak States, for Example Do Not Cite without Prior Permission from Authors the Illusion of Equality: the Educational Consequences of Blinding Weak States, for Example

Does the government control of school systems facilitate equality in school quality? There is a trade-off. On the one hand, government direct control of schools, typically through a large scale hierarchical organization, could produce equalization across schools by providing uniformity in inputs, standards, and teacher qualifications that localized individually managed schools could not achieve. But there is a tendency for large scale formal bureaucracies to " see " less and less of localized reality and hence to manage on the basis of a few simple, objective, and easily administratively verified characteristics (e.g. resources per student, formal teacher qualifications). Whether centralized or localized control produces more equality depends therefore not only on what " could " happen in principle but what does happen in practice. When government implementation capacity is weak, centralized control could lead to only the illusion of equality: in which central control of education with weak internal or external accountability actually allows for much greater inequalities across schools than entirely " uncontrolled " local schools. Data from Pakistan, using results from the LEAPS study, and from two states of India show much larger variance in school quality (adjusted for student characteristics) among the government schools—because of very poor public schools which continue in operation. We use the PISA data to estimate school specific learning achievement (in mathematics, science, and reading) net of individual student and school average background characteristics and compare public and private schools for 34 countries. For these countries there is, on average, exactly the same inequality in adjusted learning achievement across the private schools as across the public schools. But while inequality is the same on average, in some countries, such as Denmark, there was much more equality within the public sector while in others, such as Mexico, there was much more inequality among the public schools. Among the 18 non-OECD participating PISA countries the standard deviation across schools in adjusted quality was, on average, 36 percent higher in government than in private schools. In cases with weak states the proximate cause of high inequality again was that the public sector distribution of performance had a long left tail—schools with extremely poor performance. Relying on blinded weak states for top-down control of educational systems can be lose-lose relative to localized systems relying on bottom-up control— producing worse average performance and higher inequality. 3 A self-ordained professor's tongue Too serious to fool Spouted out that liberty …
Living with HIV, disclosure patterns and partnerships a decade after the introduction of HIV programmes in rural South Africa

Prevention of mother-to-child Transmission and HIV Treatment programmes were scaled-up in resource-constrained settings over a decade ago, but there is still much to be understood about women's experiences of living with HIV and their HIV disclosure patterns. This qualitative study explored women's experiences of living with HIV, 6–10 years after being diagnosed during pregnancy. The area has high HIV prevalence, and an established HIV treatment programme. Participants were enrolled in a larger intervention, “Amagugu”, that supported women (n = 281) to disclose their HIV status to their children. Post-intervention we conducted individual in-depth interviews with 20 randomly selected women, stratified by clinic catchment area, from the total sample. Interviews were entered into ATLAS.ti computer software for coding. Most women were living with their current sexual partner and half were still in a relationship with the child's biological father. Household exposure to HIV was high with the majority of women knowing at least one other HIV-infected adult in their household. Eighteen women had disclosed their HIV status to another person; nine had disclosed to their current partner first. Two main themes were identified in the analyses: living with HIV and the normalisation of HIV treatment at a family level; and the complexity of love relationships, in particular in long-term partnerships. A decade on, most women were living positively with HIV, accessing care, and reported experiencing little stigma. However, as HIV became normalised new challenges arose including concerns about access to quality care, and the need for family-centred care. Women's sexual choices and relationships were intertwined with feelings of love, loyalty and trust and the important supportive role played by partners and families was acknowledged, however, some aspects of living with HIV presented challenges including continuing to practise safe sex several years after HIV diagnosis.
Glucocorticoid-induced impairment in declarative memory performance in adult humans

Glucocorticoids (GCs) have a variety of effects on the brain including site-preferential, inhibitory effects on hippocampal neurons. In the case of dexamethasone (DEX), extended rather than single-dose treatment in vivo may be required for binding to brain rather than peripheral (e.g., pituitary) GC receptors and for maximizing other biologic effects in hippocampus (e.g., GC receptor downregulation, inhibition of glucose transport). Based on the contributory role of hippocampal neurons in declarative memory performance, we investigated the cognitive consequences of DEX treatment in normal adult human subjects, hypothesizing a decrease in declarative memory performance after extended but not overnight treatment. Double-blind, placebo-controlled treatment with DEX was given at 2300 hr for four consecutive days (0.5, 1, 1, 1 mg, respectively). Plasma sampling (0800 and 1600 hr) and cognitive testing (1600 hr) were performed on study days 0 (baseline), 1, and 4, and 7 d posttreatment. Repeated-measures ANOVA found a significant interaction between study day and treatment condition for correct recall during a paragraph recall task [F(3,51) = 3.52, p = 0.02]. DEX (n = 10) in comparison to placebo (n = 9) treatment decreased correct paragraph recall on study day 4 [F(1,17) = 5.01, p = 0.04] and study day 11 [F(1,17) = 5.82, p = 0.03], with the lowest level of performance occurring on day 4 followed by a return toward baseline performance level by day 11. In the placebo-treated subjects, correct paragraph recall improved over the course of treatment, consistent with practice. (ABSTRACT TRUNCATED AT 250 WORDS)
Microstructured tellurite glass fibre laser development.

ix DECLARATION . xi ACKNOWLEDGMENTS . xiii PUBLICATIONS DURING CANDIDATURE . xv LIST OF TABLES . xvii LIST OF FIGURES . xix LIST OF ABBREVIATIONS. xxix GLOSSARY . xxx
Model checking for the concurrent constraint paradigm

This paper abstracts the contents of the Ph.D. dissertation which has been recently defended by the author. Although model checking was defined to automatically verify hardware, in the last decades it has been showed that it is possible to apply the technique also to software. The concurrent constraint paradigm is a simple but powerful computational model which we can use to specify reactive and hybrid systems. The thesis considers three of the timed languages of this paradigm. It presents two methods to apply the model checking technique to two different timed concurrent constraint languages, and it is also defined a denotational semantics which is fully abstract w.r. t. the operational behavior of another timed concurrent constraint language. This new semantics allows one to perform useful static analysis of programs.
Neonatal brainstem dysfunction risks infant social engagement

The role of the brainstem in mediating social signaling in phylogenetic ancestral organisms has been demonstrated. Evidence for its involvement in social engagement in human infants may deepen the understanding of the evolutionary pathway of humans as social beings. In this longitudinal study, neonatal brainstem functioning was measured by auditory brainstem-evoked responses (ABRs) in 125 healthy neonates born prematurely before 35 weeks’ gestational age. At 4 months, infants were tested in a set of structured vignettes that required varying levels of social engagement and cardiac vagal tone was assessed. Data show that neonates with a disrupted I–V waveform, evident mostly by delayed wave V, exhibit shorter latencies to gaze averts in episodes involving direct face-to-face interactions but engage gaze as controls when interacting with masked agents or with agents whose faces are partly veiled by toys. Analysis of variance of infants’ social engagement with ABR, neonatal risk, maternal stress and cardiac vagal tone showed a main effect for ABR and an ABR by gestational age interaction. The integrity of brainstem transmission of sensory information during the final weeks of gestation may scaffold the development of social disengagement, thereby attesting to the brainstem's preserved evolutionary role in developing humans as social organisms prior to engaging in social encounters.
Immunization against a Saccharide Epitope Accelerates Clearance of Experimental Gonococcal Infection

The emergence of ceftriaxone-resistant strains of Neisseria gonorrhoeae may herald an era of untreatable gonorrhea. Vaccines against this infection are urgently needed. The 2C7 epitope is a conserved oligosaccharide (OS) structure, a part of lipooligosaccharide (LOS) on N gonorrhoeae. The epitope is expressed by 94% of gonococci that reside in the human genital tract (in vivo) and by 95% of first passaged isolates. Absence of the 2C7 epitope shortens the time of gonococcal carriage in a mouse model of genital infection. To circumvent the limitations of saccharide immunogens in producing long lived immune responses, previously we developed a peptide mimic (called PEP1) as an immunologic surrogate of the 2C7-OS epitope and reconfigured it into a multi-antigenic peptide, (MAP1). To test vaccine efficacy of MAP1, female BALB/c mice were passively immunized with a complement-dependent bactericidal monoclonal antibody specific for the 2C7 epitope or were actively immunized with MAP1. Mice immunized with MAP1 developed a TH1-biased anti-LOS IgG antibody response that was also bactericidal. Length of carriage was shortened in immune mice; clearance occurred in 4 days in mice passively administered 2C7 antibody vs. 6 days in mice administered control IgG3λ mAb in one experiment (p = 0.03) and 6 vs. 9 days in a replicate experiment (p = 0.008). Mice vaccinated with MAP1 cleared infection in 5 days vs. 9 days in mice immunized with control peptide (p = 0.0001 and p = 0.0002, respectively in two replicate experiments). Bacterial burden was lower over the course of infection in passively immunized vs. control mice in both experiments (p = 0.008 and p = 0.0005); burdens were also lower in MAP1 immunized mice vs. controls (p<0.0001) and were inversely related to vaccine antibodies induced in the vagina (p = 0.043). The OS epitope defined by mAb 2C7 may represent an effective vaccine target against gonorrhea, which is rapidly becoming incurable with currently available antibiotics.