Miracle-dz/UniFAR-FTUs huggingface dataset:
Outbreaks of antimicrobial resistant Salmonella Choleraesuis in wild boars piglets from central‐western Spain

Abstract Salmonella enterica serovar Choleraesuis is the aetiological agent of swine paratyphoid being a highly invasive zoonotic pathogen. Wild boar natural populations are experiencing a demographical expansion as well as some farms are breeding this species to release for hunting with management sometimes identical to that of domestic pigs, including supplementation, grouping, and antibiotic treatments. This situation increases the chance of contact between wild boars and livestock, and potentially induces stress, with different sanitary consequences. The present work aims to describe the clinical features of recent outbreaks caused by S. Choleraesuis in wild boar from central‐western Spain, as well as the antimicrobial resistance and phylogenetic relationships of isolates involved. 28 strains of S. Choleraesuis were isolated from 28 different wild boars belonging to 10 different game states located in central western Spain and submitted to the Clinical Veterinary Hospital (CVH) of the University of Extremadura. Samples were taken from different organs and cultured according to the ISO 6579:2002 procedure. Suspicious colonies were identified by PCR and antimicrobial resistance was evaluated by disc diffusion susceptibility test and the presence of the main resistance genes as well as 18 plasmid replicons frequently found among the Enterobacteriaceae was verified by PCR. Pulsed field gel electrophoresis was applied to determine the genetic relationship between isolates. The outbreaks under study were characterized by high mortality (35%–84%) and a septicaemic presentation. S. Choleraesuis was isolated from all the wild boars analysed, and 26 of the 28 isolates presented resistance to at least one antibiotic. The predominant resistances found were against sulphonamide, streptomycin, tetracycline, and doxicicline and sul1, strA‐strB, and tetA were the most prevalent resistance genes among isolates. 10 strains carried FIIA, FIB+H/1 or FIIA+H/1 plasmids. PFGE classified the isolates into four different profiles, grouped into two clusters. This results show that prevention against S. Choleraesuis must be considered in the sanitary programs of the wild boar breeders.
Application of the Different Electrophoresis Techniques to the Detection and Identification of Lactic Acid Bacteria in Wines

© 2012 Gonzalez-Arenzana et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Application of the Different Electrophoresis Techniques to the Detection and Identification of Lactic Acid Bacteria in Wines
Cystic echinococcosis in animals and humans of Maharashtra State, India

Echinococcosis/hydatidosis which is a neglected parasitic zoonosis in the developing country like India. The study was conducted during April, 2010 to March, 2017 to determine the prevalence among slaughtered food animals, dogs and human. The samples were collected from the various slaughterhouses situated in different regions of Maharashtra state. A total of 9464 cattle (male), 3661 buffalo, 47189 sheep, 33350 goats and 13579 pigs were scientifically examined during PM inspection at different slaughterhouses. The study revealed that the prevalence of disease in cattle (3.00%) was highest followed by buffalo (2.05%), pig (1.28%), sheep (0.09%) and goat (0.01%), by PM examination. The average estimated economic losses (direct and indirect) due to hydatidosis were Rs. 8,65,83,566 in cattle, Rs. 44,33,93,900 in buffalo, Rs. 7,24,50,615 in sheep, Rs. 1,88,29,359 in goat and Rs. 5,20,49,081 in pigs. Dog faecal samples analyzed and showed the prevalence of echinococcosis as 4.34% (19/438) by sedimentation method and positive samples were confirmed by PCR assay, whereas in high risk human, 11.09% sera samples were found to be positive for echinococcosis. However, based on data collection for seven years, 58 patients were found to be surgically operated for hydatid cyst removal. The results of the present study indicated that cystic echinococcosis/hydatidosis is prevalent in both human and animal population in study areas which attracts serious attention from veterinary and public health authority to reduce economic burden and in designing appropriate strategy for prevention and control of disease.
Environmental management model for small dairies in the Industrial Corridor of Boyaca (Colombia)

This research addresses the general objective of environmental issues, focusing on the Environmental Management System model that can be applied to small businesses in the dairy industry in the Industrial Corridor of Boyaca, for which the Environmental Management Model based on the NTC ISO 14000 Standard was created. This is a case study of 10 small dairy companies legally established in the study area. The research results are presented initially with a description of the context and specific means of dairy production activity in the Industrial Corridor of Boyaca; a situational diagnosis was performed for the dairy chain of the department, and likewise the study configured the profile and characterization of the administration and environment of these companies in the study area, using the information collected through a survey of the businesses as the input. With the information obtained in the diagnosis, the Environmental Management Model for these organizations was designed.
Postprandial nutrient-sensing and metabolic responses after partial dietary fishmeal replacement by soyabean meal in turbot (Scophthalmus maximus L.)

Abstract In this study, we chose a carnivorous fish, turbot (Scophthalmus maximus L.), to examine its nutrient-sensing and metabolic responses after ingestion of diets with fishmeal (FM), or 45 % of FM replaced by soyabean meal (34·6 % dry diet) balanced with or without essential amino acids (EAA) to match the amino acid profile of FM diet for 30 d. After a 1-month feeding trial, fish growth, feed efficiency and nutrient retention were markedly reduced by soyabean meal-incorporated (SMI) diets. Compared with the FM diet, SMI led to a reduction of postprandial influx of free amino acids, hypoactivated target of rapamycin signalling and a hyperactivated amino acid response pathway after refeeding, a status associated with reduced protein synthesis, impaired postprandial glycolysis and lipogenesis. These differential effects were not ameliorated by matching an EAA profile of soyabean meal to that of the FM diet through dietary amino acid supplementation. Therefore, this study demonstrated that the FM diet and SMI diets led to distinct nutrient-sensing responses, which in turn modulated metabolism and determined the utilisation efficiency of diets. Our results provide a new molecular explanation for the role of nutrient sensing in the inferior performance of aquafeeds in which FM is replaced by soyabean meal.
Evaluation of Beeswax Influence on Physical Properties of Lipstick Using Instrumental and Sensory Methods

The aim of this study was to optimize the lipsticks formulation according to the physical properties and sensory attributes and investigate the relationship between instrumental and sensory analyses and evaluate the influence of the main ingredients, beeswax and oil, with analysis of lipsticks properties. Central composite design was used to optimize the mixture of oils and beeswax and cocoa butter for formulation of lipsticks. Antioxidant activity was evaluated by DPPH free radical scavenging method spectrophotometrically. Physical properties of lipsticks melting point were determined in a glass tube; the hardness was investigated with texture analyzer. Sensory analysis was performed with untrained volunteers. The optimized mixture of sea buckthorn oil and grapeseed oil mixture ratio 13.96 : 6.18 showed the highest antioxidative activity (70 ± 0.84%) and was chosen for lipstick formulation. According to the sensory and instrumental analysis results, optimal ingredients amounts for the lipstick were calculated: 57.67% mixture of oils, 19.58% beeswax, and 22.75% cocoa butter. Experimentally designed and optimized lipstick formulation had good physical properties and high scored sensory evaluation. Correlation analysis showed a significant relationship between sensory and instrumental evaluations.
Learning Generative Models with Sinkhorn Divergences

The ability to compare two degenerate probability distributions (i.e. two probability distributions supported on two distinct low-dimensional manifolds living in a much higher-dimensional space) is a crucial problem arising in the estimation of generative models for high-dimensional observations such as those arising in computer vision or natural language. It is known that optimal transport metrics can represent a cure for this problem, since they were specifically designed as an alternative to information divergences to handle such problematic scenarios. Unfortunately, training generative machines using OT raises formidable computational and statistical challenges, because of (i) the computational burden of evaluating OT losses, (ii) the instability and lack of smoothness of these losses, (iii) the difficulty to estimate robustly these losses and their gradients in high dimension. This paper presents the first tractable computational method to train large scale generative models using an optimal transport loss, and tackles these three issues by relying on two key ideas: (a) entropic smoothing, which turns the original OT loss into one that can be computed using Sinkhorn fixed point iterations; (b) algorithmic (automatic) differentiation of these iterations. These two approximations result in a robust and differentiable approximation of the OT loss with streamlined GPU execution. Entropic smoothing generates a family of losses interpolating between Wasserstein (OT) and Maximum Mean Discrepancy (MMD), thus allowing to find a sweet spot leveraging the geometry of OT and the favorable high-dimensional sample complexity of MMD which comes with unbiased gradient estimates. The resulting computational architecture complements nicely standard deep network generative models by a stack of extra layers implementing the loss function.
Optical properties of aerosol mixtures derived from sun-sky radiometry during SAMUM-2

The SAMUM-2 experiment took place in the Cape Verde is lands in January–February 2008. The colocated ground-based and airborne instruments allow the study of desert dust optical and microphysical properties in a closure experiment. The Meteorological Institute of the University of Munich deployed one sun-sky photometer and two tropospheric lidar systems. A travelling AERONET-Cimel sun-sky radiometer was also deployed. During the measurement period the aerosol scenario over Cape Verde mostly consisted of a dust layer below 2 km and a smoke-dust layer above 2–4 km a.s. l. The Saharan dust arrived at the site from the NE, whereas the smoke originated in the African equatorial region. This paper describes the main results of the Sun photometer observations, supported by lidar information. An analysis of the variations in the aerosol optical depth (AOD) in the range 340–1550 nm, the Ångström exponent, volume size distributions and single scattering albedo is presented. The aerosol mixtures are analysed by means of the fine mode fraction of the AOD provided by the sun-sky inversion data and the Spectral Deconvolution Algorithm. The mean AOD (500 nm) was 0.31, with associated low ångström exponent of 0.46. Several types of events were detected within the data set, with prevalence of dust or mixtures as characterized by the Ångstr¨om exponents of extinction and absorption and the fine mode fraction. Aerosol properties derived from sunphotometry were compared to in situ measurements of size distribution, effective radius and single scattering albedo.
CXCL4 Plasma Levels Are Not Associated with the Extent of Coronary Artery Disease or with Coronary Plaque Morphology

Background CXCL4 is a platelet chemokine released at micromolar concentrations upon platelet activation. CXCL4 has been shown to promote atherogenesis by various mechanisms. However, data on CXCL4 plasma levels in patients with coronary artery disease are largely inconclusive. Computed coronary artery angiography (CCTA) represents an excellent tool to quantify and characterize coronary atherosclerotic plaques. We hypothesized that increased CXCL4 plasma levels may be associated with features of plaque instability resulting in adverse cardiovascular events. Specifically, we sought to determine whether CXCL4 levels are correlated with specific features of coronary artery disease including (1) plaque volume, (2) calcium score, (3) degree of stenosis, or (4) vascular remodeling. Methods and Results CXCL4 plasma levels were measured by ELISA in 217 patients undergoing CCTA for suspected CAD (mean age 64.2 ± 9.4 years, 107 (49.3%) male). Mean CXCL4 plasma levels were 12.5 ± 4.6 ng/mL. There was no significant correlation between CXCL4 levels and any clinical or demographic parameters including cardiovascular risk factors. CXCL4 plasma levels did not differ between patient with or without coronary artery disease (CAD: 12.5 ± 4.5 ng/ml, no CAD: 12.5 ± 4.8 ng/ml). Neither univariate nor multivariate analysis showed an association between CXCL4 levels and plaque volume, total calcium score, degree of stenosis, or vascular remodeling. Subgroup analysis of patients with CAD as confirmed by CCTA did not show any association of CXCL4 levels with the extent of CAD. Conclusions While CXCL4 may be present and active within the arterial wall, local increase of CXCL4 may not translate into systemically elevated CXCL4 levels. Further studies will have to test whether CXCL4 may still represent a suitable therapeutic target in human atherosclerosis.
RF behavior of a 220/251.5 GHz, 2MW, triangular corrugated coaxial cavity gyrotron extended to the third operating frequency 283 GHz

In this paper, RF behavior studies of a 2MW triangular corrugated coaxial cavity gyrotron is presented for the operating frequency of 283 GHz to explore the possibility of multi-frequency operation in an already proposed dual regime gyrotron (220/251.5 GHz). The cavity mode for the 283 GHz operation is chosen as TE62,38. Cold cavity analysis, mode competition studies and self consistent single mode calculations are carried out for this operating frequency. Time dependent multi-mode calculations are performed to verify power growth in the desired mode and the possibility of power in the competing modes. Startup analyses are carried out using the electron beam parameters obtained from the electron gun design studies for both partial and complete space charge neutralization cases. These studies confirm that 2MW, continuous wave (CW) operation can be obtained at all the three operating frequencies (220/251.5/283 GHz) in the proposed coaxial cavity gyrotron.
Rapid Identification of Intact Staphylococcal Bacteriophages Using Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry

Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities. Five different phage purification techniques were tested in combination with two MALDI-TOF MS matrices. Phages, either purified by CsCl density gradient centrifugation or as resuspended phage pellets, yielded mass spectra with the highest information value if ferulic acid was used as the MALDI matrix. Phage tail and capsid proteins yielded the strongest signals whereas the culture conditions had no effect on mass spectral quality. Thirty-seven phages from Myoviridae, Siphoviridae or Podoviridae families were analysed, including 23 siphophages belonging to the International Typing Set for human strains of S. aureus, as well as phages in preparations produced by Microgen, Bohemia Pharmaceuticals and MB Pharma. The data obtained demonstrate that MALDI-TOF MS can be used to effectively distinguish between Staphylococcus-specific bacteriophages.
Assessment of reporting sites for acute flaccid paralysis surveillance in Ethiopia: implications for planning of active case search visits

Introduction The World Health Organization acute flaccid paralysis (AFP) surveillance standards recommend documentation of the role of each potentially reporting site for evidence -based planning and tailoring support for active surveillance visits. This study assessed the contribution of various sites as source and quality of AFP cases reported over a five -year period in Ethiopia. Methods We conducted a retrospective analysis of AFP surveillance data collected from 2010-2014 in Ethiopia. Analyses were done using EPI-INFO version 7 for calculating frequencies and proportions, and testing possible associations between reporting sites and key dependent variables. Results Of the 5,274 AFP cases reported, hospitals and health centers reported 4627 (88%) of the cases. Hospitals in Addis Ababa (53%) and health posts in Benishangul Gumuz (48%) regions have contributed majority of the cases reported. Only 3% of cases were reported by private clinics nationally. The stool adequacy rate for health posts (81%) was lower than the overall national rate of 88% . Cases from health posts are more likely to be reported after 14 days of onset of paralysis, and 62% less likely to be investigated within two days of notification(OR: 1.82, 95% CI OR : 1.41-2.36, p-value <0.0001). Greater proportion (2.4%) of cases reported from health posts were either compatible, VDPV or WPV compared to cases reported by health centers (1.14%) or hospitals (1.4%). Conclusion Though majority of the cases were reported by health centers followed by hospitals ,our findings suggest that all potentially reporting sites should be exhaustively identified, prioritized and regularly supported for quality case detection, investigation and reporting.
The Evolution of Polystyrene as a Cell Culture Material

Polystyrene (PS) has brought in vitro cell culture from its humble beginnings to the modern era, propelling dozens of research fields along the way. This review discusses the development of the material, fabrication, and treatment approaches to create the culture material. However, native PS surfaces poorly facilitate cell adhesion and growth in vitro. To overcome this, liquid surface deposition, energetic plasma activation, and emerging functionalization methods transform the surface chemistry. This review seeks to highlight the many potential applications of the first widely accepted polymer growth surface. Although the majority of in vitro research occurs on two-dimensional surfaces, the importance of three-dimensional (3D) culture models cannot be overlooked. The methods to transition PS to specialized 3D culture surfaces are also reviewed. Specifically, casting, electrospinning, 3D printing, and microcarrier approaches to shift PS to a 3D culture surface are highlighted. The breadth of applications of the material makes it impossible to highlight every use, but the aim remains to demonstrate the versatility and potential as both a general and custom cell culture surface. The review concludes with emerging scaffolding approaches and, based on the findings, presents our insights on the future steps for PS as a tissue culture platform.
Design and performance evaluation of a switched-beam antenna array for 60 GHz WPAN applications

In this paper, the design and performance evaluation of a switched-beam antenna array to operate in the 60 GHz band for short-range WPAN applications is presented. In order to overcome the high path-loss attenuation at millimeter-wave frequencies and, moreover, to achieve high data rate wireless communications, highly directive antennas are required. In addition, beam-steering capabilities are also needed to deal with the high user random mobility and human-body shadowing characteristic of indoor environments. For these reasons, we propose a design based on a five by five slot antenna array, in which each single element can be selected individually, and a dielectric inhomogeneous gradient-index flat lens to steer and enhance the radiation in a particular direction. A total of 25 fixed high-gain beams can be selected individually to scan in both theta and phi directions. Our measurement results show that we can achieve up to 17.25 dB of broadside gain, and beam-steering capabilities from +20° to -20° with more than 15 dB of gain, and up to ±52° with around 12.8 dB, with low-side lobe levels, in the entire frequency band of interest (from 57 to 64 GHz).
Betrayed by Your Dashboard: Discovering Malicious Campaigns via Web Analytics

To better understand the demographics of their visitors and their paths through their websites, the vast majority of modern website owners make use of third-party analytics platforms, such as, Google Analytics and ClickTale. Given that all the clients of a third-party analytics platform report to the same server, the tracking requests need to contain identifiers that allow the analytics server to differentiate between their clients. In this paper, we analyze the analytics identifiers utilized by eighteen different third-party analytics platforms and show that these identifiers enable the clustering of seemingly unrelated websites as part of a common third-party analytics account (i.e. websites whose analytics are managed by a single person or team). We focus our attention on malicious websites that also utilize third-party web analytics and show that threat analysts can utilize web analytics to both discover previously unknown malicious pages in a threat-agnostic fashion, as well as to cluster malicious websites into campaigns. We build a system for automatically identifying, isolating, and querying analytics identifiers from malicious pages and use it to discover an additional 11K live domains that use analytics associated with malicious pages. We show how our system can be used to improve the coverage of existing blacklists, discover previously unknown phishing campaigns, identify malicious binaries and Android apps, and even aid in attribution of malicious domains with protected WHOIS information.
Understanding disentangling in β-VAE

We present new intuitions and theoretical assessments of the emergence of disentangled representation in variational autoencoders. Taking a rate-distortion theory perspective, we show the circumstances under which representations aligned with the underlying generative factors of variation of data emerge when optimising the modified ELBO bound in $\beta$-VAE, as training progresses. From these insights, we propose a modification to the training regime of $\beta$-VAE, that progressively increases the information capacity of the latent code during training. This modification facilitates the robust learning of disentangled representations in $\beta$-VAE, without the previous trade-off in reconstruction accuracy.