Miracle-dz/UniFAR-FTUs huggingface dataset:
Laboratory evaluation of the effects of aggregated gradation and binder type on performance of asphalt mixtures

Findings from a laboratory study on the performance of seven different asphalt mixtures are presented. The mixtures were developed by systematically changing aggregate gradations and binders to investigate the effects of these mixture variables on performance. Dynamic Shear Rheometer (DSR) and Bending Beam Rheometer (BBR) tests were conducted on the binders to obtain temperature susceptibility. Axial creep, triaxial repeated permanent deformation, and uniaxial tensile fatigue tests were performed on the mixtures. Fatigue data were analyzed using the viscoelastic, continuum damage approach. The results from the experiments were investigated together to evaluate the effects of different gradations and asphalt modifications.
High-Frequency Jump Filtering in a Microstructure Model ∗

We estimate a general microstructure model with transitory and permanent order flow price impact written as a state-space model. We distinguish jumps in the price (observation) equation and in the fundamental value (state) equation and introduce information about the size and direction of the trades. We find that buy and sell orders have an asymmetric price impact. Jumps barely affect the estimation of the microstructure parameters. Explicit modeling of microstructure effects decreases the standard deviation of innovations and therefore more jumps will be detected. On average we detect about one jump per day. We obtain similar numbers of occurrences of both types of jumps with increased intensity in the morning and the close. By casting the model in a Bayesian OLS setting with intradaily volatility seasonality, we are able to estimate the intradaily evolution of market characteristics.
Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA

ABSTRACT Hepatitis C virus (HCV) NS5B protein is the viral RNA-dependent RNA polymerase capable of directing RNA synthesis. In this study, an electrophoretic mobility shift assay demonstrated the interaction between a partially purified recombinant NS5B protein and a 3′ viral genomic RNA with or without the conserved 98-nucleotide tail. The NS5B-RNA complexes were specifically competed away by the unlabeled homologous RNA but not by the viral 5′ noncoding region and very poorly by the 3′ conserved 98-nucleotide tail. A 3′ coding region with conserved stem-loop structures rather than the 3′ noncoding region of the HCV genome is critical for the specific binding of NS5B. Nevertheless, no direct interaction between the 3′ coding region and the HCV NS5A protein was detected. Furthermore, two independent RNA-binding domains (RBDs) of NS5B were identified, RBD1, from amino acid residues 83 to 194, and RBD2, from residues 196 to 298. Interestingly, the conserved motifs of RNA-dependent RNA polymerase for putative RNA binding (220-DxxxxD-225) and template/primer position (282-S/TGxxxTxxxNS/T-292) are present in the RBD2. Nevertheless, the RNA-binding activity of RBD2 was abolished when it was linked to the carboxy-terminal half of the NS5B. These results provide some clues to understanding the initiation of HCV replication.
On a theorem of Kontsevich

In [12] and [13], M. Kontsevich introduced graph homology as a tool to compute the homology of three infinite dimensional Lie algebras, associated to the three operads "commutative," "associative" and "Lie. " We generalizehis theorem to all cyclic operads, in the process giving a more careful treatment of the construction than in Kontsevich's original papers. We also give a more explicit treatment of the isomorphisms of graph homologies with the homology of moduli space and Out(F r ) outlined by Kontsevich. In [4] we defined a Lie bracket and cobracket on the commutative graph complex, which was extended in [3] to the case of all cyclic operads. These operations form a Lie bi-algebra on a natural subcomplex. We show that in the associative and Lie cases the subcomplex on which the bi-algebra structure exists carries all of the homology, and we explain why the subcomplex in the commutative case does not.
The symbiotic binary system RX Puppis: a possible recurrent nova with a Mira companion

We present an analysis of photometric and spectroscopic observations of the symbiotic binary system RX Pup with the aims of developing a reliable binary model for the system and identifying mechanisms responsible for its spectacular activity. The binary is composed of a long-period Mira variable surrounded by a thick dust shell and a hot ∼ 0.8M ⊙ white dwarf companion. The hot component produces practically all activity observed in the UV, optical and radio range, while variable obscuration of the Mira by circumstellar dust is responsible for long-term changes in the near-infrared magnitudes. The observations show RX Pup underwent a nova-like eruption during the last three decades. The hot component contracted in radius at roughly constant luminosity from 1975 to 1986, and was the source of a strong stellar wind which prevented it from accreting material lost in the Mira wind. Around 1988/9 the hot component turned over in the HR diagram and by 1991 its luminosity had faded by a factor of ∼ 30 with respect to the maximum plateau value and the hot wind had practically ceased. By 1995 the nova remnant started to accrete material from the Mira wind, as indicated by a general increase in intensity of the optical continuum and H i emission. The quiescent spectrum resembles the quiescent spectra of symbiotic recurrent novae, and its intensity indicates the hot component must accrete as much as ∼ 1 per cent of the Mira wind, which is more or less the amount predicted by Bondi-Hoyle theory. The earliest observational records from the 1890s suggest that another nova-like eruption of RX Pup occurred around 1894.
High and Low Gain Switches for Regulation of cAMP Efflux Concentration: Distinct Roles for Particulate GC- and Soluble GC-cGMP-PDE3 Signaling in Rabbit Atria

Abstract— This study tests the hypothesis that particulate (p) guanylyl cyclase (GC) and soluble (s) GC are involved in the distinct roles for the regulation of cGMP-PDE-cAMP signaling and of mechanical and secretory functions in the heart. Experiments were performed in perfused beating rabbit atria. C-type natriuretic peptide (CNP) and SIN-1, an NO donor, or BAY 41-2272 (BAY), a direct activator for sGC, were used to activate pGC and sGC, respectively. CNP and SIN-1 increased cGMP and cAMP efflux in a concentration-dependent manner. Increase in cAMP was a function of cGMP. The changes in cAMP efflux concentration in terms of cGMP were much more prominent in the atria treated with CNP than in the atria treated with SIN-1. Increase in cAMP efflux concentration was blocked by milrinone but not changed by EHNA. BAY increased cGMP but not cAMP in a concentration-dependent manner. CNP and SIN-1 decreased atrial stroke volume and myocytic ANP release. The decreases in terms of cGMP efflux concentration were much more prominent in the atria treated with CNP than in the atria treated with SIN-1 or BAY. Milrinone accentuated GC agonist–induced decreases in atrial stroke volume and ANP release. In the presence of ODQ, SIN-1 or BAY induced effects were not observed. These data suggest that pGC and sGC activations have distinct roles via cGMP-PDE3-cAMP signaling in the cardiac atrium: high and low gain switches, respectively, for the regulation of cAMP levels and contractile and secretory functions.
Gene expression in mature neutrophils: early responses to inflammatory stimuli

Neutrophils provide an essential defense against bacterial and fungal infection and play a major role in tissue damage during inflammation. Using oligonucleotide microarrays, we have examined the time course of changes in gene expression induced by stimulation with live, opsonized Escherichia coli, soluble lipopolysaccharide, and the chemoattractant formyl‐methionyl‐leucyl‐phenylalanine. The results indicate that activated neutrophils generate a broad and vigorous set of alterations in gene expression. The responses included changes in the levels of transcripts encoding 148 transcription factors and chromatin‐remodeling genes and 95 regulators of protein synthesis or stability. Clustering analysis showed distinct temporal patterns with many rapid changes in gene expression within the first hour of exposure. In addition to the temporal clustering of genes, we also observed rather different profiles associated with each stimulus, suggesting that even a nonvirulent organism such as E. coli is able to play a dynamic role in shaping the inflammatory response. Principal component analysis of transcription factor genes demonstrated clear separation of the neutrophil‐response clusters from those of resting and stimulated human monocytes. The present study indicates that combinatorial transcriptional regulation including alterations of chromatin structure may play a role in the rapid changes in gene expression that occur in these terminally differentiated cells.
Direct Parsing of Discontinuous Constituents in German

Discontinuities occur especially frequently in languages with a relatively free word order, such as German. Generally, due to the longdistance dependencies they induce, they lie beyond the expressivity of Probabilistic CFG, i.e., they cannot be directly reconstructed by a PCFG parser. In this paper, we use a parser for Probabilistic Linear Context-Free Rewriting Systems (PLCFRS), a formalism with high expressivity, to directly parse the German NeGra and TIGER treebanks. In both treebanks, discontinuities are annotated with crossing branches. Based on an evaluation using different metrics, we show that an output quality can be achieved which is comparable to the output quality of PCFG-based systems.
Role of microtubules in polarized delivery of apical membrane proteins to the brush border of the intestinal epithelium

Colchicine- and vinblastine-induced depolymerization of microtubules (MTs) in the intestinal epithelium of rats and mice resulted in significant delivery of three apical membrane proteins (alkaline phosphatase, sucrase-isomaltase, and aminopeptidase N) to the basolateral membrane domain. In addition, typical brush borders (BBs) occurred at the basolateral cell surface, consisting of numerous microvilli that contained the four major components of the cytoskeleton of apical microvilli (actin, villin, fimbrin, and the 110-kD protein). Formation of basolateral microvilli required polymerization of actin and proceeded at glycocalyx-studded plaques that resembled the dense plaques located at the tips of apical microvilli. BBs from the basolateral membrane became internalized into BB-containing vacuoles which served as recipient organelles for newly synthesized apical membrane proteins. The BB vacuoles fused with each other and finally were inserted into the apical BB. Polarized distribution of Na+,K+- ATPase, a basolateral membrane protein, was not affected by drug- induced depolymerization of MTs. These observations indicate that Golgi- derived carrier vesicles (CVs) containing apical membrane proteins are vectorially guided to the apical cell surface by a retrograde transport along MTs. MTs are uniformly oriented towards a narrow space underneath the apical terminal web (termed subterminal space) that contains MT- organizing properties and controls polarized alignment of MTs. In contrast to apical CVs, targeting of basolateral CVs appears to be independent of MTs but demands a barrier at the apical membrane domain that prevents basolateral CVs from apical fusion (transport barrier hypothesis).
Temperature Dependence of Voltage-gated H+ Currents in Human Neutrophils, Rat Alveolar Epithelial Cells, and Mammalian Phagocytes

H+ currents in human neutrophils, rat alveolar epithelial cells, and several mammalian phagocyte cell lines were studied using whole-cell and excised-patch tight-seal voltage clamp techniques at temperatures between 6 and 42°C. Effects of temperature on gating kinetics were distinguished from effects on the H+ current amplitude. The activation and deactivation of H+ currents were both highly temperature sensitive, with a Q 10 of 6–9 (activation energy, E a, ≈ 30–38 kcal/mol), greater than for most other ion channels. The similarity of E a for channel opening and closing suggests that the same step may be rate determining. In addition, when the turn-on of H+ currents with depolarization was fitted by a delay and single exponential, both the delay and the time constant (τact) had similarly high Q 10. These results could be explained if H+ channels were composed of several subunits, each of which undergoes a single rate-determining gating transition. H+ current gating in all mammalian cells studied had similarly strong temperature dependences. The H+ conductance increased markedly with temperature, with Q 10 ≥ 2 in whole-cell experiments. In excised patches where depletion would affect the measurement less, the Q 10 was 2.8 at >20°C and 5.3 at <20°C. This temperature sensitivity is much greater than for most other ion channels and for H+ conduction in aqueous solution, but is in the range reported for H+ transport mechanisms other than channels; e.g., carriers and pumps. Evidently, under the conditions employed, the rate-determining step in H+ permeation occurs not in the diffusional approach but during permeation through the channel itself. The large E a of permeation intrinsically limits the conductance of this channel, and appears inconsistent with the channel being a water-filled pore. At physiological temperature, H+ channels provide mammalian cells with an enormous capacity for proton extrusion.
Predictive error detection in pianists: a combined ERP and motion capture study

Performing a piece of music involves the interplay of several cognitive and motor processes and requires extensive training to achieve a high skill level. However, even professional musicians commit errors occasionally. Previous event-related potential (ERP) studies have investigated the neurophysiological correlates of pitch errors during piano performance, and reported pre-error negativity already occurring approximately 70–100 ms before the error had been committed and audible. It was assumed that this pre-error negativity reflects predictive control processes that compare predicted consequences with actual consequences of one's own actions. However, in previous investigations, correct and incorrect pitch events were confounded by their different tempi. In addition, no data about the underlying movements were available. In the present study, we exploratively recorded the ERPs and 3D movement data of pianists' fingers simultaneously while they performed fingering exercises from memory. Results showed a pre-error negativity for incorrect keystrokes when both correct and incorrect keystrokes were performed with comparable tempi. Interestingly, even correct notes immediately preceding erroneous keystrokes elicited a very similar negativity. In addition, we explored the possibility of computing ERPs time-locked to a kinematic landmark in the finger motion trajectories defined by when a finger makes initial contact with the key surface, that is, at the onset of tactile feedback. Results suggest that incorrect notes elicited a small difference after the onset of tactile feedback, whereas correct notes preceding incorrect ones elicited negativity before the onset of tactile feedback. The results tentatively suggest that tactile feedback plays an important role in error-monitoring during piano performance, because the comparison between predicted and actual sensory (tactile) feedback may provide the information necessary for the detection of an upcoming error.
Inhibition of Melanoma Angiogenesis by Telomere Homolog Oligonucleotides

Telomere homolog oligonucleotides (T-oligos) activate an innate telomere-based program that leads to multiple anticancer effects. T-oligos act at telomeres to initiate signaling through the Werner protein and ATM kinase. We wanted to determine if T-oligos have antiangiogenic effects. We found that T-oligo-treated human melanoma (MM-AN) cells had decreased expression of vascular endothelial growth factor (VEGF), VEGF receptor 2, angiopoeitin-1 and -2 and decreased VEGF secretion. T-oligos activated the transcription factor E2F1 and inhibited the activity of the angiogenic transcription factor, HIF-1α. T-oligos inhibited EC tubulogenesis and total tumor microvascular density matrix invasion by MM-AN cells and ECs in vitro. In melanoma SCID xenografts, two systemic T-oligo injections decreased by 60% (P < .004) total tumor microvascular density and the functional vessels density by 80% (P < .002). These findings suggest that restriction of tumor angiogenesis is among the host's innate telomere-based anticancer responses and provide further evidence that T-oligos may offer a powerful new approach for melanoma treatment.
Risk hull method and regularization by projections of ill-posed inverse problems

We study a standard method of regularization by projections of the linear inverse problem Y = Af + ∈, where ∈ is a white Gaussian noise, and A is a known compact operator with singular values converging to zero with polynomial decay. The unknown function f is recovered by a projection method using the singular value decomposition of A. The bandwidth choice of this projection regularization is governed by a data-driven procedure which is based on the principle of risk hull minimization. We provide nonasymptotic upper bounds for the mean square risk of this method and we show, in particular, that in numerical simulations this approach may substantially improve the classical method of unbiased risk estimation.
The effect of endotoxin on the controllability of cardiac rhythm in rats

Decreased heart rate variability (HRV) has both diagnostic and prognostic value in patients with sepsis. However, it is not known whether reduced HRV in sepsis reflects an altered input from the autonomic nervous system or a remodeling of the cardiac pacemaker cells by inflammatory mediators. The present study aimed to investigate the effect of endotoxin on the heart rate dynamics of a denervated isolated heart in rats. Saline or endotoxin was injected into rats and their hearts were isolated and perfused. Atrial electrical activity was recorded and memory length in the time-series was assessed using inverse statistical analysis. Memory was defined as a statistical feature that lasts for a period of time and distinguishes the time-series from a random process. Endotoxaemic hearts exhibited a prolonged memory compared to the controls with respect to observing rare events. This indicates that a sudden decelerating event could potentially affect the cardiac rhythm of an endotoxaemic heart for a longer time than the controls. The prolongation of memory is indirectly linked to a reduced controllability in a complex system; therefore our data may provide evidence for a reduced controllability in cardiac rhythm following endotoxaemia.
Elasticity of α-Cristobalite: A Silicon Dioxide with a Negative Poisson's Ratio

Laser Brillouin spectroscopy was used to determine the adiabatic single-crystal elastic stiffness coefficients of silicon dioxide (SiO2) in the α-cristobalite structure. This SiO2 polymorph, unlike other silicas and silicates, exhibits a negative Poisson's ratio; α-cristobalite contracts laterally when compressed and expands laterally when stretched. Tensorial analysis of the elastic coefficients shows that Poisson's ratio reaches a maximum value of –0.5 in some directions, whereas averaged values for the single-phased aggregate yield a Poisson's ratio of –0.16.
Formation of fully pearlitic microstructure in medium carbon steel

Pearlitic microstructure can be obtained when austenite of eutectoid composition is slowly cooled from high temperature to below the Ae3 temperature. It is also possible to induce such structure in hypo-eutectoid composition of austenite through proper heat treatment as well. However, in the current work, one hypo-eutectoid steel during very slow cooling only produced completely pearlitic microstructure which was not expected from a steel with such composition. The calculations carried out considering orthoequilibrium condition actually predicted the presence of about 37% ferrite in the microstructure. Further calculations considering different equilibrium modes and kinetics of transformation indicates that the composition and thermal condition of the steel under consideration was such that proeutectoid ferrite formation could not start before the composition reaches to the Negligible Partitioning Local Equilibrium phase boundary which further coincides with the area described by Hultgren extrapolation and thus, the steel completely transforms to pearlite even with hypo-eutectoid composition during very slow cooling.
High Temperature Stability of SiC/Ti Interface

Ti, SiC and their composite materials have been widely used as high temperature structural material. The knowledge of interfacial stability between SiC and Ti is vital in high temperature applications. In this study, SiC/Ti diffusion couples were prepared to investigate the interfacial reactions between SiC and Ti at 1273 K. Phase forming sequence, microstructure and thermal stability of SiC/Ti interface were studied. It was indicated that after annealed at 1273 K for 10 days, 4 reaction layers were formed at the SiC/Ti interface. The diffusion path between SiC and Ti is SiC/Ti3SiC2/Ti5Si3/Ti5Si3+TiC/Ti3Si/Ti. As the annealing time prolong, the thicknesses of these reaction layers increased.
Parry–Romberg syndrome: A possible association with borreliosis

Progressive facial hemiatrophy or Parry–Romberg syndrome is a rare entity characterized by unilateral atrophy of the skin, subcutaneous tissue and the underlying bony structures. This syndrome has many features of linear scleroderma ‘en coup de sabre’ but is distinguished by more extensive involvement of the lower face with only slight cutaneous sclerosis. The onset typically occurs in childhood or young adult years. The aetiology of the condition is unknown. A case of progressive hemifacial atrophy occurring in a 30‐year‐old woman is reported, in which the aetiology is thought to be Lyme disease, a borrelial infection first recognized in 1975 that has since become the most commonly reported vector‐borne disease in the world. No sure link is established between these two disease states, but their coincident occurrence in this patient is noted. We hypothesize that the aetiology of Parry–Romberg syndrome involves borreliosis.
Shape modeling with point-sampled geometry

We present a versatile and complete free-form shape modeling framework for point-sampled geometry. By combining unstructured point clouds with the implicit surface definition of the moving least squares approximation, we obtain a hybrid geometry representation that allows us to exploit the advantages of implicit and parametric surface models. Based on this representation we introduce a shape modeling system that enables the designer to perform large constrained deformations as well as boolean operations on arbitrarily shaped objects. Due to minimum consistency requirements, point-sampled surfaces can easily be re-structured on the fly to support extreme geometric deformations during interactive editing. In addition, we show that strict topology control is possible and sharp features can be generated and preserved on point-sampled objects. We demonstrate the effectiveness of our system on a large set of input models, including noisy range scans, irregular point clouds, and sparsely as well as densely sampled models.