Miracle-dz/UniFAR-FTUs huggingface dataset:
Transplants and Neurotrophic Factors Prevent Atrophy of Mature CNS Neurons after Spinal Cord Injury

Axotomy of mature rubrospinal neurons leads to a substantial atrophy of these neurons within days after surgery. In addition, these neurons do not successfully regenerate following axotomy. The relationship of atrophy to regenerative failure is not clear, and the signals which regulate these events have not been identified. However, it is possible that the atrophy of these neurons plays a role in preventing regeneration. In the present study, we evaluated the hypothesis that interventions which have been shown to promote growth of axotomized CNS neurons are also capable of reversing the axotomy-induced atrophy. To test this hypothesis, adults rats received thoracic spinal cord hemisection alone or in combination with transplants of fetal spinal cord tissue and/or neurotrophic factor support. Our data indicate that application of either transplants or neurotrophic factors partially reverse the axotomy-induced atrophy in rubrospinal neurons, but that both interventions together reverse the atrophy completely. These results suggest that the same pathways that are activated to enhance growth of rubrospinal neurons after axotomy may also be involved in the maintenance of cell morphology.
Unpicking the Semantic Impairment in Alzheimer’s Disease: Qualitative Changes with Disease Severity

Despite a vast literature examining semantic impairment in Alzheimer's disease (AD), consensus regarding the nature of the deficit remains elusive. We re-considered this issue in the context of a framework that assumes semantic cognition can break down in two ways: (1) core semantic representations can degrade or (2) cognitive control mechanisms can become impaired [1]. We hypothesised and confirmed that the nature of semantic impairment in AD changes with disease severity. Patients at mild or severe stages of the disorder exhibited impairment across various semantic tasks but the nature of those deficits differed qualitatively for the two groups. Commensurate with early dysfunction of the cognitive control, temporoparietal-frontal-cingulate network, characteristics of deregulated semantic cognition were exhibited by the mild AD cases. In contrast, the severe AD group reproduced features of additional degradation of core semantic representations. These results suggest that spread of pathology into lateral anterior temporal lobes in later stage AD produces degradation of semantic representations, exacerbating the already deregulated system. Moreover, the dual nature of severe patients’ impairment was highlighted by disproportionately poor performance on tasks placing high demand on both conceptual knowledge and control processes–e.g., category fluency.
The relationship between emotional intelligence and customer orientation for pharmaceutical salespeople

Purpose – The purpose of this paper is to examine the relationships between salesperson emotional intelligence, dispositional affectivity, and customer‐orientation levels in pharmaceutical marketing.Design/methodology/approach – A total of 71 pharmaceutical salespeople working in the UK provided responses to scales designed to assess emotional intelligence, dispositional affectivity (positive affect (PA) and negative affect (NA) and customer orientation). The emotional intelligence and dispositional affectivity scores provided by the salespeople were then analyzed to determine the degree to which they related to customer‐orientation levels.Findings – The findings indicate that salesperson emotional intelligence levels are positively correlated with their customer‐orientation scores. Positive dispositional affectivity levels are also significantly correlated with salesperson customer‐orientation levels. This result suggests that UK pharmaceutical salespeople who possessed more PA tended to also be more pos.
Investigating the potential of re-conductoring a lattice tower overhead line structure

In order to evaluate the potential from re-conductoring existing overhead power systems (OHL), the paper employs a holistic computational methodology that allows sag-ampacity-tension calculations considering electro-mechanical properties of arbitrary OHL systems. The focus of analysis is a 275 kV lattice tower system. Initial investigation of the performance of the widely used aluminum alloy conductors (AAAC) on this system and the comparison with the corresponding performance of the conductors on a 33-kV wood pole structure supports the conclusion that the OHL structure is an essential part of the system performance. Further analysis and results involve the mechanical and electrical performance of standard as well as some recently developed high temperature low sag (HTLS) composite conductors in order to identify the optimal conductor size and type for the investigated lattice tower system.
Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium

Histidine-containing phosphocarrier protein (HPr) is common to all of the phosphoenolpyruvate:sugar phosphotransferase systems (PTS) in Escherichia coli and Salmonella typhimurium, except the fructose-specific PTS. Strains which lack HPr activity (ptsH) have been characterized in the past, and it has proved difficult to delineate between tight and leaky mutants. In this study four different parameters of ptsH strains were measured: in vitro sugar phosphorylation activity of the mutant HPr; detection of 32P-labeled P-HPr; ability of monoclonal antibodies to bind mutant HPr; and sensitivity of ptsH strains to fosfomycin. Tight ptsH strains could be defined; they were fosfomycin resistant and produced no HPr protein or completely inactive mutant HPr. All leaky ptsH strains were fosfomycin sensitive, usually produced normal amounts of mutant HPr protein, and had low but measurable activity, and HPr was detectable as a phosphoprotein. This indicates that the regulatory functions of the PTS require a very low level of HPr activity (about 1%). The antibodies used to detect mutant HPr in crude extracts were two monoclonal immunoglobulin G antibodies Jel42 and Jel44. Both antibodies, which have different pIs, inhibited PTS sugar phosphorylation assays, but the antibody-HPr complex could still be phosphorylated by enzyme I. Preliminary evidence suggests that the antibodies bind to two different epitopes which are in part located in a beta-sheet structure.
About fractional models physical consistency: Case of implicit differentiation based fractional order models

As recently shown, a fractional model can be viewed as a doubly infinite model: its “real state” is of infinite dimension as it is distributed, but it is distributed on an infinite domain. It is shown in the paper, that this last feature induces a physically inconsistent property: the model real state has the ability to store an infinite amount of energy. This property demonstration is based on an electrical interpretation of fractional models. As a consequence, even if fractional models permit to capture accurately the input-output dynamical behavior of many physical systems, such a property highlights a physical inconsistence of fractional models: they do not reflect the reality of macroscopic physical systems in terms of energy storage ability. This property is shown for implicit fractional models and extends previous result of the authors for explicit fractional models.
Effect of insertion of xanthan-based chlorhexidine gel in the maintenance phase following the treatment of chronic periodontitis

Objectives: The aim of this study was to evaluate the efficacy of subgingivally administered xanthan-based chlorhexidine gel when used in the maintenance phase following scaling and root planing (SRP) in the treatment of chronic periodontitis. Materials and Methods: A randomized, controlled, single-center study was conducted involving 92 sites in 46 systemically healthy patients suffering from moderate to advanced chronic periodontitis with isolated pockets. The selected sites were randomized to two treatment arms: Group A (SRP alone) and Group B (SRP + insertion of chlorhexidine gel after 1 month). The gingival index, plaque index, probing pocket depth (PPD) and clinical attachment level (CAL) were recorded at baseline and subsequently after 1 month and 3 months. Results: Both the groups showed significant reductions in PPD and CAL at both follow-up visits when compared with the baseline values (P<0.001). Conclusions: The results suggest that the application of xanthan based chlorhexidine gel following SRP in the maintenance phase might be beneficial in treatment of the chronic periodontitis in comparison to SRP alone. Greater improvements may be achieved when antimicrobial agents are used following SRP.
Generating Scenario Trees for Multistage Decision Problems

In models of decision making under uncertainty we often are faced with the problem of representing the uncertainties in a form suitable for quantitative models. If the uncertainties are expressed in terms of multivariate continuous distributions, or a discrete distribution with far too many outcomes, we normally face two possibilities: either creating a decision model with internal sampling, or trying to find a simple discrete approximation of the given distribution that serves as input to the model. This paper presents a method based on nonlinear programming that can be used to generate a limited number of discrete outcomes that satisfy specified statistical properties. Users are free to specify any statistical properties they find relevant, and the method can handle inconsistencies in the specifications. The basic idea is to minimize some measure of distance between the statistical properties of the generated outcomes and the specified properties. We illustrate the method by single- and multiple-period problems. The results are encouraging in that a limited number of generated outcomes indeed have statistical properties that are close to or equal to the specifications. We discuss how to verify that the relevant statistical properties are captured in these specifications, and argue that what are the relevant properties, will be problem dependent.
Global pose estimation with limited GPS and long range visual odometry

Here we present an approach to estimate the global pose of a vehicle in the face of two distinct problems; first, when using stereo visual odometry for relative motion estimation, a lack of features at close range causes a bias in the motion estimate. The other challenge is localizing in the global coordinate frame using very infrequent GPS measurements. Solving these problems we demonstrate a method to estimate and correct for the bias in visual odometry and a sensor fusion algorithm capable of exploiting sparse global measurements. Our graph-based state estimation framework is capable of inferring global orientation using a unified representation of local and global measurements and recovers from inaccurate initial estimates of the state, as intermittently available GPS information may delay the observability of the entire state. We also demonstrate a reduction of the complexity of the problem to achieve real-time throughput. In our experiments, we show in an outdoor dataset with distant features where our bias corrected visual odometry solution makes a fivefold improvement in the accuracy of the estimated translation compared to a standard approach. For a traverse of 2km we demonstrate the capabilities of our graph-based state estimation approach to successfully infer global orientation with as few as 6 GPS measurements and with two-fold improvement in mean position error using the corrected visual odometry.
Getting to Know You: Reputation and Trust in a Two-Person Economic Exchange

Using a multiround version of an economic exchange (trust game), we report that reciprocity expressed by one player strongly predicts future trust expressed by their partner—a behavioral finding mirrored by neural responses in the dorsal striatum. Here, analyses within and between brains revealed two signals—one encoded by response magnitude, and the other by response timing. Response magnitude correlated with the “intention to trust” on the next play of the game, and the peak of these “intention to trust” responses shifted its time of occurrence by 14 seconds as player reputations developed. This temporal transfer resembles a similar shift of reward prediction errors common to reinforcement learning models, but in the context of a social exchange. These data extend previous model-based functional magnetic resonance imaging studies into the social domain and broaden our view of the spectrum of functions implemented by the dorsal striatum.
Model‐based linkage analysis with imprinting for quantitative traits: ignoring imprinting effects can severely jeopardize detection of linkage

Genes with imprinting (parent‐of‐origin) effects express differently when inheriting from the mother or from the father. Some genes for development and behavior in mammals are known to be imprinted. We developed parametric linkage analysis that accounts for imprinting effects for continuous traits, implementing it in MORGAN. To study misspecification of imprinting on linkage analysis, we simulated eight markers over a 35 cM region with phenotypes where imprinting contributes 0, 25, 50, and 75% of the variance of a quantitative trait locus (QTL) effect and analyzed them under all four models. Multipoint lod scores were computed and maximized over the same 35 cM region. Our most important finding is the dramatic lod score improvement under the correct imprinting model over the no‐imprinting model. For data with minor QTL allele frequency 0.05, the correct model provided the highest lod scores with maximum expected lod scores over 4 in all settings. Ignoring imprinting provided the lowest lod scores with maximum expected lod scores between −9.9 and 2.4. In the extreme scenario, cases with max lod ≥3 from the correct imprinting model and max lod ≤−2 from the no‐imprinting model occurred in 86% of replications. Models with misspecified imprinting produced lod scores intermediate between those with correct imprinting and with no imprinting. The effects of model misspecification were less pronounced for singlepoint analysis. Our multipoint results illustrate that ignoring true imprinting severely impairs detection of linkage and erroneously excludes genomic regions (with max lod <−2), whereas accounting for it can substantially improve linkage detection. Genet. Epidemiol. 2008. © 2008 Wiley‐Liss, Inc.
Robust mean field games for coupled Markov jump linear systems

ABSTRACT We consider robust stochastic large population games for coupled Markov jump linear systems (MJLSs). The N agents’ individual MJLSs are governed by different infinitesimal generators, and are affected not only by the control input but also by an individual disturbance (or adversarial) input. The mean field term, representing the average behaviour of N agents, is included in the individual worst-case cost function to capture coupling effects among agents. To circumvent the computational complexity and analyse the worst-case effect of the disturbance, we use robust mean field game theory to design low-complexity robust decentralised controllers and to characterise the associated worst-case disturbance. We show that with the individual robust decentralised controller and the corresponding worst-case disturbance, which constitute a saddle-point solution to a generic stochastic differential game for MJLSs, the actual mean field behaviour can be approximated by a deterministic function which is a fixed-point solution to the constructed mean field system. We further show that the closed-loop system is uniformly stable independent of N, and an approximate optimality can be obtained in the sense of ε-Nash equilibrium, where ε can be taken to be arbitrarily close to zero as N becomes sufficiently large. A numerical example is included to illustrate the results.
Long-Term Cardiac pro-B-Type Natriuretic Peptide Gene Delivery Prevents the Development of Hypertensive Heart Disease in Spontaneously Hypertensive Rats

Background— Diastolic dysfunction associated with high blood pressure (BP) leads to cardiac remodeling and fibrosis and progression to congestive heart failure. B-type natriuretic peptide (BNP) has BP-lowering, antifibrotic, and antihypertrophic properties, which makes BNP an attractive agent for attenuating the adverse cardiac remodeling associated with hypertension. In the current study, we tested the effects of sustained cardiac proBNP gene delivery on BP, cardiac function, and remodeling in spontaneously hypertensive rats (SHR). Methods and Results— We used the myocardium-tropic adeno-associated virus serotype 9 (AAV9) vector to achieve continuously enhanced cardiac rat proBNP expression. In SHR, a single systemic administration of AAV9 vector allowed long-term cardiac BNP overexpression, resulting in reductions in systolic and diastolic BP for 9 months after injection. Left ventricular (LV) thickness, LV end-systolic dimensions, and LV mass were reduced, whereas ejection fraction was significantly increased, in BNP-treated compared with untreated SHR. Circumferential systolic strain and strain rate of the early phase of diastole were improved in BNP-treated compared with untreated SHR. Noncardiac overexpression of BNP via AAV2 vector was not associated with changes in BP and plasma BNP in SHR. Furthermore, normal Wistar rats injected with AAV9 proBNP vector showed significantly reduced heart weights 4 weeks after injection without BP reduction. Conclusions— AAV9 vector facilitates sustained cardiac proBNP overexpression and improves LV function in hypertensive heart disease. Long-term proBNP delivery improved both systolic and diastolic function. The effects on cardiac structure and function occurred independently of BP-lowering effects in normal Wistar rats.
Ambulatory Care of the Pregnant Diabetic

One hundred eight pregnancies in 98 insulin-dependent diabetic women were managed according to a strict protocol calling for weekly postprandial blood sugar determinations, frequent prenatal contacts, and placental evaluation. Patients were cared for on an ambulatory basis with an average of 1 hospitalization during pregnancy exclusive of delivery. There were no fetal deaths and only 1 neonatal death related to diabetes, for a corrected perinatal mortality of 0.97%. White's classification was not helpful in identifying perinatal risks. The incidence of both perinatal and maternal morbidity was high; however, complications were not serious and the overall outcome was acceptable. In view of the financial and social benefits to patients, it is recommended that an ambulatory approach to care of the pregnant diabetic patient be used and when feasible it should be the method of choice.
Uncertainty and primary appraisal as predictors of acute stress disorder in parents of critically ill children: A mediational model

UNCERTAINTY AND PRIMARY APPRAISALS AS PREDICTORS OF ACUTE STRESS DISORDER IN PARENTS OF CRITICALLY ILL CHILDREN: A MEDIATIONAL MODEL By Monica Mary Durrette, M.S. A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University. Virginia Commonwealth University, 2013 Major Director: Marilyn Stern, Ph.D. Professor Departments of Psychology, Pediatrics, Social and Behavioral Health This study examined illness-related uncertainty and primary appraisals of threat, centrality, and challenge as predictors of acute stress disorder (ASD) symptoms in parents of children hospitalized in a pediatric intensive care unit (PICU). Ultimately, a mediational pathway was tested to determine if primary appraisal was a mechanism that accounted for the impact of uncertainty on ASD symptoms. Ancillary study aims were to assess the degree to which parents perceived uncertainty in the PICU environment, and to determine the prevalence of ASD among parents in this setting. Self-report data was collected from 77 parents (57 mothers, 19 fathers) of children hospitalized in a PICU for a minimum of 48 hours. Descriptive analyses showed that parents perceived a high degree of uncertainty and 57% of parents met diagnostic criteria for ASD. Unexpected admission was the only objective medical status variable significantly related to uncertainty, threat appraisal, and ASD symptoms. Consistent with hypotheses, results from hierarchical regression analyses showed that perceived uncertainty and primary appraisals of threat accounted for significant variance in parents’ ASD symptoms; however, neither centrality nor challenge appraisals were related to parents’ ASD symptoms. Because threat was the only appraisal dimension found to be directly related to ASD symptoms, it was the only dimension tested in the mediational model. Consistent with the hypothesis, threat appraisals fully mediated the effect of uncertainty on ASD symptoms; results from a Sobel test confirmed the significance of full mediation. This study is the first to examine uncertainty, primary appraisal, and ASD symptoms in this population. Results clarify that it is not the mere perception of uncertainty that adversely impacts parental adjustment, but rather how it is appraised, and therefore, point to a practical area for in-hospital interventions targeting parents’ pediatric medical traumatic stress symptoms. Although a substantial body of empirical research supports a relation between uncertainty and maladaptive outcomes, studies also link uncertainty to positive outcomes. Future research should include positive indicators of adjustment and examine how appraisals vary according to sources of uncertainty.
Bacteriological quality and food safety in a Brazilian school food program.

INTRODUCTION Food safety is a critical issue in school food program. OBJECTIVE This study was conducted to assess the bacteriological quality and food safety practices of a municipal school food program (MSFP) in Jequitinhonha Valley, Brazil. MATERIALS AND METHODS A checklist based on good manufacturing practices (GMP) for food service was used to evaluate food safety practices. Samples from foods, food contact surfaces, the hands of food handlers, the water supply and the air were collected to assess bacteriological quality in establishments that comprise the MSFP. RESULTS Nine (81.8%) establishments were classified as poor quality and two (18.2%) as medium quality. Neither Salmonella nor Listeria monocytogenes were detected in food samples. Coliforms, Escherichia coli and Staphylococcus aureus were detected in 36 (52.9%), 1 (1.5%) and 22 (32.4%) of the food samples and in 24 (40.7%), 2 (3.3%) and 13 (22.0%) of the food contact surfaces, respectively. The counts of coliforms and Staphylococcus aureus ranged from 1 to 5.0 and 1 to 5.1 log CFU/g of food, respectively. The mean aerobic mesophilic bacteria count was 3.1 log CFU/100 cm2 of surface area. Coliforms, E. coli and S. aureus were detected on the hands of 33 (73.3%), 1 (2.2%) and 36 (80%) food handlers, respectively. With regard to air quality, all the establishments had an average aerobic mesophilic count above 1.6 log CFU/cm2/week. CONCLUSIONS The results indicate the need to modify the GMP used in food service in MSFP in relation to food safety, particularly because children served in these establishments are often the most socially vulnerable.
Gfap and Osmr regulation by BRG1 and STAT3 via interchromosomal gene clustering in astrocytes

Gene clustering is relevant in the regulation of gene expression. However, the mechanisms of gene clustering remain to be elucidated. Using a glial differentiation system, we found that the clustering of Gfap, an astrocyte-pecific gene, with Osmr enhances transcription of both genes. BRG1 and the JAK-STAT pathway are central to the clustering.