Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytosolic free Ca2+ changes and calpain activation are required for β integrin–accelerated phagocytosis by human neutrophils

Phagocytosis of microbes coated with opsonins such as the complement component C3bi is the key activity of neutrophils. However, the mechanism by which opsonins enhance the rate of phagocytosis by these cells is unknown and has been difficult to study, partly because of the problem of observing and quantifying the events associated with phagocytosis. In this study, C3bi-opsonized particles were presented to neutrophils with a micromanipulator, so that the events of binding, pseudopod cup formation, engulfment, and completion of phagocytosis were clearly defined and distinguished from those involved with chemotaxis. Using this approach in combination with simultaneous phase contrast and Ca2+ imaging, the temporal relationship between changes in cytosolic free Ca2+ concentration and phagocytosis were correlated. Here we show that whereas small, localized Ca2+ changes occur at the site of particle attachment and cup formation as a result of store release, rapid engulfment of the particle required a global change in cytosolic free Ca2+ which resulted from Ca2+ influx. This latter rise in cytosolic free Ca2+ concentration also liberated a fraction of β2 integrin receptors which were initially immobile on the neutrophil surface, as demonstrable by both fluorescence recovery after laser bleaching and by visualization of localized β2 integrin labelling. Inhibitors of calpain activation prevented both the Ca2+-induced liberation of β2 integrin and the rapid stage of phagocytosis, despite the persistence of the global Ca2+ signal. Therefore, we propose that Ca2+ activation of calpain causes β2 integrin liberation, and that this signal plays a key role in the acceleration of β2 integrin–mediated phagocytosis.
The role of non‐native plants and vertebrates in defining patterns of compositional dissimilarity within and across continents

Aim Human activities have led to the spread and establishment of increasing numbers of non-native species. Here we assess whether non-native plant and vertebrate species have affected species compositions within and across Europe and North America. We also assess the effects of intra-continental species exchange using the example of vertebrates. Location European countries and North America (states in the contiguous United States and provinces of Canada). Methods We measured compositional dissimilarity of native and non-native assemblages of vascular plants and vertebrates and related these patterns to climatic dissimilarity and geographical distance. We considered three categories of non-native species (introduced after ad 1500), namely: those (1) originating outside of both continents, (2) native to one continent and non-native to the other, and (3) native in a particular region of a continent but non-native in another region. Results The presence of non-native plants and vertebrates led to more homogeneous species compositions between continents and to less homogeneous species composition within Europe compared with the native assemblages. In North America, the presence of non-native plants led to more homogeneous species compositions and the presence of non-native vertebrates had no effect. Species compositions being more homogeneous than the native composition were found for the three categories of non-native vertebrate species for both continents. Between continents, climate was a better predictor of compositional dissimilarity for non-native plants, whereas for vertebrates the explanatory power of climate and geographical distance were comparable. By contrast, within continents, climate was a better predictor of compositional dissimilarity of both plants and vertebrates. Conclusions We found clear evidence for biotic homogenization as a consequence of species displacement. However, in relation to overall species richness this effect was rather small, indicating that floras and faunas are still quite distinct. Therefore, claiming that we already face homogeneous biotas might be premature, although clear indications are visible which should raise a note of caution, especially in the light of increasing globalization.
Evidence for quantitative trait loci affecting ovulation rate in pigs.

Fifty-five microsatellite markers were scored in an F2 population of pigs (n = 114 females) developed at the University of Nebraska. The population was produced by crossing a line previously selected for 10 generations using an index of ovulation rate and embryonal survival and a line selected at random. The lines were from a common base population and differed by 6.7 ovulations and 3.1 fetuses at 50 d of gestation. Ovulation rate and number of fully formed and mummified pigs were recorded for each female. Data were analyzed with regression models that included random animal effects. Likelihood-ratio tests were used to test for quantitative trait loci (QTL) effects by deviating the log-likelihood for the full model that included additive and dominance QTL effects from the log-likelihood for the reduced model that did not contain QTL effects. A QTL for ovulation rate was found on chromosome 8 (P < .001) with an additive effect of 3.07 ovulations. Other evidence of potential QTL affecting ovulation rate was found on chromosomes 4 (P < .10), 13, and 15 (P < .05). Effects on chromosomes 4, 13 and 15 were not significant for fan experiment-wise threshold value of P < . 001. No significant QTL for litter size or number of mummified pigs were found. Additional data are needed to confirm the location and the effect of QTL found for ovulation rate before markers associated with them can be used in marker-assisted selection.
High-dose intravenous infusion of irrigating fluids containing glycine and mannitol in the pig.

BACKGROUND Large-scale absorption of electrolyte-free irrigating fluid during endoscopic surgery may result in a "transurethral resection syndrome. " The severity of the syndrome can probably be modified by using mannitol 5% instead of the most widely used glycine 1.5%. METHODS Seventeen pigs with a mean body weight of 22 (range 19-26) kg received an intravenous infusion of 100 mL kg(-1) h(-1) of either glycine 1.5% or mannitol 5% over 90 min. Central hemodynamics, whole-body and brain oxygen consumption, intracranial pressure, blood hemoglobin, and the sodium concentrations in serum and urine were monitored for 120 min. Selected measurements were made on 6 other pigs given mannitol 3% and in 2 controls not given any fluid. Morphological examinations of the hearts were conducted. RESULTS Both glycine 1.5% and mannitol 5% transiently increased cardiac output, the aortic blood flow rate, and arterial pressures, but all of these parameters fell to below baseline after the infusions were ended. The intracranial pressure was lower (P < 0.05) and the oxygen consumption in the brain decreased (P < 0.001) during the infusion of mannitol 5%. Glycine 1.5% expanded the intracellular volume more than mannitol did (P < 0.002). Signs of myocardial damage were graded glycine 1.5% > mannitol 5% > mannitol 3%. CONCLUSIONS Massive infusion of glycine 1.5% and mannitol 5% left the pigs in a hypokinetic hypotensive state. Glycine 1.5% increased the intracranial pressure and injured the myocardium more than mannitol 5%, which then seems to be a more appropriate irrigating fluid to use during endoscopic surgery.
Monte Carlo techniques for time-dependent radiative transfer in 3-D supernovae

Monte Carlo techniques based on indivisible energy packets are described for computing light curves and spectra for 3-D supernovae. The radiative transfer is time-dependent and includes all effects of O(v/c). Monte Carlo quantization is achieved by discretizing the initial distribution of 56 Ni into N radioactive pellets. Each pellet decays with the emission of a single energy packet comprising γ-ray photons representing one line from either the 56 Ni or the 56 Co decay spectrum. Subsequently, these energy packets propagate through the homologously-expanding ejecta with appropriate changes in the nature of their contained energy as they undergo Compton scatterings and pure absorptions. The 3-D code is tested by applying it to a spherically-symmetric SN in which the transfer of optical radiation is treated with a grey absorption coefficient. This 1-D problem is separately solved using Castor's co-moving frame moment equations. Satisfactory agreement is obtained. The Monte Carlo code is a platform onto which more advanced treatments of the interactions of matter and radiation can be added. Some of these have already been developed and tested in previous papers and are summarized here.
The effect of energy drinks on the urge to drink alcohol in young adults.

BACKGROUND Recently, Marczinski and colleagues (2013) showed that energy drinks combined with alcohol augment a person's desire to drink more alcohol relative to drinking alcohol alone. The current study replicates the findings of Marczinski and colleagues (2013) using a robust measure of alcohol craving. METHODS Seventy-five participants aged 18 to 30 years were assigned to an alcohol only or alcohol+energy drink condition in a double-blind randomized pre- versus posttest experiment. Participants received a cocktail containing either 60 ml of vodka and a Red Bull(®) Silver Edition energy drink (alcohol+energy drink condition) or 60 ml of vodka with a soda water vehicle (alcohol-only condition); both cocktails contained 200 ml of fruit drink. The primary outcome measure was the Alcohol Urge Questionnaire taken at pretest and at 20 minutes (posttest). Other measures taken at posttest were the Biphasic Alcohol Effects Questionnaire, the Drug Effects Questionnaire, and breath alcohol concentration (BAC). RESULTS The alcohol+energy drink condition showed a greater pre- versus posttest increase in urge to drink alcohol compared with the alcohol-only condition (B = 3.24, p = 0.021, d = 0.44). Participants in the alcohol+energy drink condition had significantly higher ratings on liking the cocktail and wanting to drink more of the cocktail, and lower BACs, than the alcohol-only condition. When examined at specific BACs, the effect of the energy drink on the pre- to posttest increase in urge to drink was largest and only significant at BACs of 0.04-0.05 (cf. < 0.04 g/dl). There were no significant differences in stimulation, sedation, feeling the effects of the cocktail, or feeling high. CONCLUSIONS Combining energy drinks with alcohol increased the urge to drink alcohol relative to drinking alcohol alone. More research is needed to understand what factors mediate this effect and whether it increases subsequent alcohol consumption.
Automated deconvolution and deisotoping of electrospray mass spectra.

Electrospray ionization (ESI) of peptides and proteins produces a series of multiply charged ions with a mass/charge (m/z) ratio between 500 and 2000. The resulting mass spectra are crowded by these multiple charge values for each molecular mass and an isotopic cluster for each nominal m/z value. Here, we report a new algorithm simultaneously to deconvolute and deisotope ESI mass spectra from complex peptide samples based on their mass-dependent isotopic mean pattern. All signals corresponding to one peptide in the sample were reduced to one singly charged monoisotopic peak, thereby significantly reducing the number of signals, increasing the signal intensity and improving the signal-to-noise ratio. The mass list produced could be used directly for database searching. The developed algorithm also simplified interpretation of fragment ion spectra of multiply charged parent ions.
Portuguese crypto-Jews: the genetic heritage of a complex history

The first documents mentioning Jewish people in Iberia are from the Visigothic period. It was also in this period that the first documented anti-Judaic persecution took place. Other episodes of persecution would happen again and again during the long troubled history of the Jewish people in Iberia and culminated with the Decrees of Expulsion and the establishment of the Inquisition: some Jews converted to Catholicism while others resisted and were forcedly baptized, becoming the first Iberian Crypto-Jews. In the 18th century the official discrimination and persecution carried out by the Inquisition ended and several Jewish communities emerged in Portugal. From a populational genetics point of view, the worldwide Diaspora of contemporary Jewish communities has been intensely studied. Nevertheless, very little information is available concerning Sephardic and Iberian Crypto-Jewish descendants. Data from the Iberian Peninsula, the original geographic source of Sephardic Jews, is limited to two populations in Portugal, Belmonte, and Bragança district, and the Chueta community from Mallorca. Belmonte was the first Jewish community studied for uniparental markers. The construction of a reference model for the history of the Portuguese Jewish communities, in which the genetic and classical historical data interplay dynamically, is still ongoing. Recently an enlarged sample covering a wide region in the Northeast Portugal was undertaken, allowing the genetic profiling of male and female lineages. A Jewish specific shared female lineage (HV0b) was detected between the community of Belmonte and Bragança. In contrast to what was previously described as a hallmark of the Portuguese Jews, an unexpectedly high polymorphism of lineages was found in Bragança, showing a surprising resistance to the erosion of genetic diversity typical of small-sized isolate populations, as well as signs of admixture with the Portuguese host population.
The presence of IgA on the surface of rat thoractic duct lymphocytes which contain internal IgA

The presence of lymphocytes with internal IgA among cells from rat thoracic duct lymph wdy. The number of cells detected was greater in animals kept in a convential animal house compared with those maintained under specific pathogen-free conditions. Thoracic duct lymph from B rats and adult thymectomized rats also contained cell with internal IgA. The surface Ig of the IgA-containing cells was studied using a double-labeling technique with (126I) anti-Ig to detect surface Ig, and fluorescein-conjugated anti-IgA in large amounts, but very little IgM and no surface IgG2. The surface IgA was not acquired passively.
Effect of UV treatment on the dielectric strength of BOPP capacitor film

The effect of UV treatment on dielectric breakdown strength of metallized biaxially-oriented polypropylene (BOPP) capacitor film was studied. Results from Weibull statistical analysis indicate that an optimal UV exposure level of ~3.4 J/cm2 (200-400 nm) increases the breakdown strength at 1% probability by ~20%. UV spectroscopic analysis indicates that the antioxidant in BOPP was consumed almost entirely. The results suggest that antioxidant remaining in the BOPP after processing may have an unfavorable effect on the breakdown strength of the film
Zoroastrians Support Oocyte and Embryo Donation Program for Infertile Couples

Background The main goal was to evaluate the attitudes and knowledge of Zoroastrians living in Iran towards oocyte donation (OD) and embryo donation (ED) program. Methods This cross sectional study consisted of 318 Zoroastrians (n=175 for OD and n=143 for ED) of both sexes. The questionnaire form comprised two parts of general demographic characteristics of the participants and twenty multiple-choice questions about attitude and knowledge of participants towards OD and ED. For statistical analysis, the chi-square test was applied for comparison of data generated from ED and OD groups. Results Majority of the participants supported OD (69.7%) and ED (71.3%) for infertile patients. In addition, 40% and 42% preferred donation program (OD and ED, respectively), compared to adoption. About 60% of the respondents believed that the donors have no right to find the child and claim it as their own. In addition, more than half of the respondents thought that the recipients of oocyte/embryo should never know the name and address of the donors. More than half of the participants did not know whether their religion accepts donation program or not. Approximately, 80% of respondents supported psychological counseling for both donors and recipients. Moreover, about 56% of the participants necessitated the advertisement on OD/ED program in the mass media. Conclusion Our preliminary data showed that Zoroastrians supported both OD and ED program equally for infertile couples.
Preschoolers' appreciation of uncommon desires and subsequent emotions

Many theory of mind researchers have argued that even preschoolers understand the causal relationship between desires and emotions: the fulfilment of a desire results in a positive emotion, whereas its frustration elicits a negative emotion. Children can acknowledge this simple link between desires and emotions, even when their own desire differs from that of the story protagonist. However, in this paper we argue that under some conditions preschool children will not base their emotion predictions for another person on the basis of the other person's desires. In the first experiment, 3-to 5-year-old children were tested for their understanding of desires, when the protagonist's desire for a snack increasingly conflicted with their own preference for a snack. Only the 4-year-olds performed as expected: they gave more accurate emotion predictions when the distance between their own preference and the protagonist's desire was reduced. When the snacks were replaced by toys, however, preschool children showed a bias in their emotion predictions that seemed gender related. The second experiment confirmed that sex-stereotyped beliefs about desirability biased children's predictions of others' emotions: 4- and 5-year-old children were more accurate in their predictions when the protagonist had a traditional desire (a girl wanting to play with a doll), than when the protagonist had a non-traditional desire (a boy wanting to play with a doll), irrespective of children's own preferences for one toy over the other. In sum, evidence was found for two biasing influences in children's understanding of others' emotions: (1) an increased distance between the protagonist's desire and participants' own desires, and (2) beliefs about desirability based on, for example, cultural-norms for gender related preferences, which increases with age.
A case of oral lichen sclerosus with gingival involvement and Borrelia identification

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Statistical analysis of glottal pulses in speech under psychological stress

In this paper, the problem of speech signal under psychological stress is addressed. The investigation into the speaker's stress is based on statistical analysis of glottal pulse derivative extracted from the vowel signals. A pitch synchronous selection of segments from the glottal pulse waveform is used. Selected segments are fixed in their maxima and overlaid. The generated distribution matrix is analysed using special cuts. A new database of speech under stress is created for use in our experiments consisting of data collected during oral final examinations at our university. The database contains read and conversational speech of 31 male speakers, both in neutral and in stressed state. The stress recognition rate in the speaker dependent binomial classification (stress/no stress) reaches 88%.
Executive Ownership and Control in Newly Public Firms: The Role of Venture Capitalists

We study the implications of CEO equity ownership for incentives and control in a sample of 1,011 newly public firms. Before an initial public offering, equity investments by venture capitalists reduce CEO ownership by about half, from an average of 35 percent to 19 percent. Venture capitalists narrow this difference by granting options, reducing secondary sales, and lowering the dilution by primary shares, but a gap in post-IPO CEO equity ownership remains. The effect of this lower ownership on incentives depends upon the measure employed - the dollar sensitivity of CEO pay to firm value is lower in venture firms, but the elasticity is about the same. In addition, we present evidence that lower ownership, combined with concentrated outside holdings, leads to a reduction in the agency costs of managerial control. We conclude that the patterns of ownership in part represent a tradeoff by venture capitalists between the benefits of incentives and the agency costs of control.
Structure and superorganization of acetylcholine receptor–rapsyn complexes

The scaffolding protein at the neuromuscular junction, rapsyn, enables clustering of nicotinic acetylcholine receptors in high concentration and is critical for muscle function. Patients with insufficient receptor clustering suffer from muscle weakness. However, the detailed organization of the receptor–rapsyn network is poorly understood: it is unclear whether rapsyn first forms a wide meshwork to which receptors can subsequently dock or whether it only forms short bridges linking receptors together to make a large cluster. Furthermore, the number of rapsyn-binding sites per receptor (a heteropentamer) has been controversial. Here, we show by cryoelectron tomography and subtomogram averaging of Torpedo postsynaptic membrane that receptors are connected by up to three rapsyn bridges, the minimum number required to form a 2D network. Half of the receptors belong to rapsyn-connected groups comprising between two and fourteen receptors. Our results provide a structural basis for explaining the stability and low diffusion of receptors within clusters.