Miracle-dz/UniFAR-FTUs huggingface dataset:
Exosomes serve as novel modes of tick-borne flavivirus transmission from arthropod to human cells and facilitates dissemination of viral RNA and proteins to the vertebrate neuronal cells

Molecular determinants and mechanisms of arthropod-borne flavivirus transmission to the vertebrate host are poorly understood. In this study, we show for the first time that a cell line from medically important arthropods, such as ticks, secretes extracellular vesicles (EVs) including exosomes that mediate transmission of flavivirus RNA and proteins to the human cells. Our study shows that tick-borne Langat virus (LGTV), a model pathogen closely related to tick-borne encephalitis virus (TBEV), profusely uses arthropod exosomes for transmission of viral RNA and proteins to the human- skin keratinocytes and blood endothelial cells. Cryo-electron microscopy showed the presence of purified arthropod/neuronal exosomes with the size range of 30 to 200 nm in diameter. Both positive and negative strands of LGTV RNA and viral envelope-protein were detected inside exosomes derived from arthropod, murine and human cells. Detection of Nonstructural 1 (NS1) protein in arthropod and neuronal exosomes further suggested that exosomes contain viral proteins. Viral RNA and proteins in exosomes derived from tick and mammalian cells were secured, highly infectious and replicative in all tested evaluations. Treatment with GW4869, a selective inhibitor that blocks exosome release affected LGTV loads in both arthropod and mammalian cell-derived exosomes. Transwell-migration assays showed that exosomes derived from infected-brain-microvascular endothelial cells (that constitute the blood-brain barrier) facilitated LGTV RNA and protein transmission, crossing of the barriers and infection of neuronal cells. Neuronal infection showed abundant loads of both tick-borne LGTV and mosquito-borne West Nile virus RNA in exosomes. Our data also suggest that exosome-mediated LGTV viral transmission is clathrin-dependent. Collectively, our results suggest that flaviviruses uses arthropod-derived exosomes as a novel means for viral RNA and protein transmission from the vector, and the vertebrate exosomes for dissemination within the host that may subsequently allow neuroinvasion and neuropathogenesis.
Evaluating Critical Uncertainty Thresholds in a Spatial Model of Forest Pest Invasion Risk

Pest risk maps can provide useful decision support in invasive species management, but most do not adequately consider the uncertainty associated with predicted risk values. This study explores how increased uncertainty in a risk model's numeric assumptions might affect the resultant risk map. We used a spatial stochastic model, integrating components for entry, establishment, and spread, to estimate the risks of invasion and their variation across a two‐dimensional landscape for Sirex noctilio, a nonnative woodwasp recently detected in the United States and Canada. Here, we present a sensitivity analysis of the mapped risk estimates to variation in key model parameters. The tested parameter values were sampled from symmetric uniform distributions defined by a series of nested bounds (±5%, … , ±40%) around the parameters' initial values. The results suggest that the maximum annual spread distance, which governs long‐distance dispersal, was by far the most sensitive parameter. At ±15% or larger variability bound increments for this parameter, there were noteworthy shifts in map risk values, but no other parameter had a major effect, even at wider bounds of variation. The methodology presented here is generic and can be used to assess the impact of uncertainties on the stability of pest risk maps as well as to identify geographic areas for which management decisions can be made confidently, regardless of uncertainty.
Dapped-End Strengthening of Full-Scale Prestressed Double Tee Beams with FRP Composites

The unusual shape of the dapped-end beam develops a severe stress concentration at the re-entrant corner. If suitable reinforcement is not provided close to the re-entrant corner, the diagonal tension crack may grow rapidly and failure may occur with little or no warning. This paper reports the testing of three full-scale prestressed concrete (PC) double tee beams (five tests) in shear at the re-entrant corner. One dapped-end of each beam was reinforced with internal steel reinforcement as a bench mark and the other was strengthened with externally bonded carbon fiber reinforced polymer (CFRP) laminates. Of the two CFRP strengthened dapped-ends one used one-ply externally bonded CFRP reinforcement and the other two-ply externally bonded CFRP reinforcement with a U-anchor. The tests revealed that the reinforcement arrangements have significant influence on the member response. For one of the CFRP cases, the application of the anchor was such that failure by fiber rupture rather than peeling was achieved. Results from design computations were confirmed.
Photocatalytic degradation of H2S in the gas-phase using a continuous flow reactor coated with TiO2-based acrylic paint

ABSTRACT For the photocatalytic degradation of the hydrogen sulphide (H2S) in the gas-phase it was developed a rectangular reactor, coated with acrylic paint supported on fiber cement material. The surface formed by the paint coverage was characterized structural and morphologically by scanning electron microscopy with energy dispersive X-ray and X-ray diffraction analysis. The flow rate and the inlet concentration of H2S were evaluated as operational performance parameters of the reactor. Removal efficiencies of up to 94% were obtained at a flow rate of 2 L min−1 (residence time of 115 s) and inlet concentration of 31 ppm of H2S. In addition, the H2S degradation kinetics was modelled according to the Langmuir–Hinshelwood (L–H) model for the inlet concentrations of 8–23 ppm of H2S. The results suggest that flow rate has a more important influence on photocatalytic degradation than the inlet concentration. It is assumed that H2S has been oxidized to , a condition that led to a deactivation of the photocatalyst after 193 min of semi-continuous use.
On Regularity of Unary Probabilistic Automata

The quantitative verification of Probabilistic Automata (PA) is undecidable in general. Unary PA are a simpler model where the choice of action is fixed. Still, the quantitative verification problem is open and known to be as hard as Skolem's problem, a problem on linear recurrence sequences, whose decidability is open for at least 40 years. In this paper, we approach this problem by studying the languages generated by unary PAs (as defined below), whose regularity would entail the decidability of quantitative verification. Given an initial distribution, we represent the trajectory of a unary PA over time as an infinite word over a finite alphabet, where the n th letter represents a probability range after n steps. We extend this to a language of trajectories (a set of words), one trajectory for each initial distribution from a (possibly infinite) set. We show that if the eigenvalues of the transition matrix associated with the unary PA are all distinct positive real numbers, then the language is effectively regular. Further, we show that this result is at the boundary of regularity, as non-regular languages can be generated when the restrictions are even slightly relaxed. The regular representation of the language allows us to reason about more general properties, e.g., robustness of a regular property in a neighbourhood around a given distribution.
High frequency variability of the Atlantic meridional overturning circulation

We compare the variability of the Atlantic meridional overturning circulation (AMOC) as simulated by the coupled climate models of the RAPID project, which cover a wide range of resolution and complexity, and observed by the RAPID/MOCHA array at about 26° N. We analyse variability on a range of timescales, from five-daily to interannual. In models of all resolutions there is substantial variability on timescales of a few days; in most AOGCMs the amplitude of the variability is of somewhat larger magnitude than that observed by the RAPID array, while the time-mean is within about 10 % of the observational estimate. The amplitude of the simulated annual cycle is similar to observations, but the shape of the annual cycle shows a spread among the models. A dynamical decomposition shows that in the models, as in observations, the AMOC is predominantly geostrophic (driven by pressure and sea-level gradients), with both geostrophic and Ekman contributions to variability, the latter being exaggerated and the former underrepresented in models. Other ageostrophic terms, neglected in the observational estimate, are small but not negligible. The time-mean of the western boundary current near the latitude of the RAPID/MOCHA array has a much wider model spread than the AMOC does, indicating large differences among models in the simulation of the wind-driven gyre circulation, and its variability is unrealistically small in the models. In many RAPID models and in models of the Coupled Model Intercomparison Project Phase 3 (CMIP3), interannual variability of the maximum of the AMOC wherever it lies, which is a commonly used model index, is similar to interannual variability in the AMOC at 26° N. Annual volume and heat transport timeseries at the same latitude are well-correlated within 15–45° N, indicating the climatic importance of the AMOC. In the RAPID and CMIP3 models, we show that the AMOC is correlated over considerable distances in latitude, but not the whole extent of the North Atlantic; consequently interannual variability of the AMOC at 50° N, where it is particularly relevant to European climate, is not well-correlated with that of the AMOC at 26° N, where it is monitored by the RAPID/MOCHA array.
Positive–Negative Asymmetry in the Evaluations of Political Candidates. The Role of Features of Similarity and Affect in Voter Behavior

In this study we followed the extension of Tversky’s research about features of similarity with its application to open sets. Unlike the original closed-set model in which a feature was shifted between a common and a distinctive set, we investigated how addition of new features and deletion of existing features affected similarity judgments. The model was tested empirically in a political context and we analyzed how positive and negative changes in a candidate’s profile affect the similarity of the politician to his or her ideal and opposite counterpart. The results showed a positive–negative asymmetry in comparison judgments where enhancing negative features (distinctive for an ideal political candidate) had a greater effect on judgments than operations on positive (common) features. However, the effect was not observed for comparisons to a bad politician. Further analyses showed that in the case of a negative reference point, the relationship between similarity judgments and voting intention was mediated by the affective evaluation of the candidate.
Tensile and fatigue behaviour of wrought magnesium alloys AZ31 and AZ61

The mechanical behaviour of two hot rolled magnesium alloys, namely the AZ31 and AZ61, has been evaluated experimentally under both monotonic and cyclic loading. Both longitudinal (L) and long transverse (LT) directions were evaluated. The tensile behaviour of the L and LT directions is similar and differs only in the offset 0.2% yield strength for both materials. This difference is attributed to the angular spread of basal poles toward the rolling direction and is more pronounced for the case of AZ31. A distinct hardening response is obvious in both directions. Twinning formation was observed; it is more pronounced in the longitudinal direction while the fracture mode is intergranular and equiaxed facets are present in the fracture surfaces of the specimens. The S-N curves exhibit a smooth transition from the low to high cycle fatigue regime. AZ61 exhibits an overall better fatigue behaviour compared to AZ31. A transgranular crack initiation mode is observed in all tested specimens while the propagation of the cracks is characterized as intergranular.
Bioactive nanocomposites for dental application obtained by reactive suspension method

Abstract Hydroxyapatite (HA) filled poly(methyl methacrylate)/poly(hydroxyethyl methacrylate) (PMMA/PHEMA) blends were prepared by reactive suspension method: HA was synthesized by co-precipitation process directly within a HEMA solution and the so-obtained suspension was polymerized in the presence of PMMA. HA particles were obtained in form of nanorods with a length of 50–200 nm and a diameter of 10–30 nm. A significant increase in glass transition temperature was observed in the nanocomposites with respect to the unfilled polymer blends. Dynamic-mechanical thermal analysis showed a significant increase in the storage modulus in the nanocomposites measured in the rubbery region. This increase was unpredicted by Mooney’s predictive equation and was attributed to the presence of cross-linking points due to the in situ generated HA particles. An increase in the elastic modulus was also observed at room temperature in compression and three-point bending tests. The presence of HA in the polymer blends resulted in an important decrease in the water sorption values. The bioactivity of the nanocomposites was verified by the precipitation of HA layer on the surface after soaking in simulated body fluid. Graphical abstract
Regenerative potential and functional composition of soil seed banks in remnant evergreen broad-leaved forests under urbanization in South China.

Soil seed banks can act as an important source in forest regeneration, and the information on the seed bank composition is vital for determining the resilience of plant communities under severe environments such as urban settings. In this study, we examined the seed bank density and functional composition, and their relationships with aboveground vegetation in three remnant evergreen broad-leaved forests, i.e., PuGang (PG), LuoGang (LG), and DaLingShan (DLS) under urbanization in Guangzhou, South China. In both years of our study (2010–2011), seed density and species richness for overall soil seed banks and each classified life forms (tree, shrub, herb and grass) significantly differed among the forests and were much higher in the PG forest. The prevailing life forms in the seed banks were herbs and grasses, and the proportion of tree species Importance Value index (IV) of the seed banks was low. We did not detect significant difference in the percentage of exotic species seeds in the seed banks among the forests. The proportion of species with animal dispersal mode was much higher in the DLS forest than in the PG and LG forests. The similarity in species composition between standing vegetation and seed banks was low with the lower value in the DLS forest than in PG and LG forest. Our findings suggest that the regeneration potential of the soil seed banks is limited for the remnant forests in urban areas. Therefore, greater proactive and enhanced conservation efforts are thus needed.
Protein Engineering for Nicotinamide Coenzyme Specificity in Oxidoreductases: Attempts and Challenges

Oxidoreductases are ubiquitous enzymes that catalyze an extensive range of chemical reactions with great specificity, efficiency, and selectivity. Most oxidoreductases are nicotinamide cofactor-dependent enzymes with a strong preference for NADP or NAD. Because these coenzymes differ in stability, bioavailability and costs, the enzyme preference for a specific coenzyme is an important issue for practical applications. Different approaches for the manipulation of coenzyme specificity have been reported, with different degrees of success. Here we present various attempts for the switching of nicotinamide coenzyme preference in oxidoreductases by protein engineering. This review covers 103 enzyme engineering studies from 82 articles and evaluates the accomplishments in terms of coenzyme specificity and catalytic efficiency compared to wild type enzymes of different classes. We analyzed different protein engineering strategies and related them with the degree of success in inverting the cofactor specificity. In general, catalytic activity is compromised when coenzyme specificity is reversed, however when switching from NAD to NADP, better results are obtained. In most of the cases, rational strategies were used, predominantly with loop exchange generating the best results. In general, the tendency of removing acidic residues and incorporating basic residues is the strategy of choice when trying to change specificity from NAD to NADP, and vice versa. Computational strategies and algorithms are also covered as helpful tools to guide protein engineering strategies. This mini review aims to give a general introduction to the topic, giving an overview of tools and information to work in protein engineering for the reversal of coenzyme specificity.
Single-Center Study Investigating Foreign Language Acquisition at School in Children, Adolescents, and Young Adults With Uni- or Bilateral Cochlear Implants in the Swiss German Population.

OBJECTIVE To evaluate foreign language acquisition at school in cochlear implant patients. STUDY DESIGN Cohort study. SETTING CI center. PATIENTS Forty three cochlear implants (CI) patients (10-18 yr) were evaluated. CI nonusers and patients with CI-explantation, incomplete datasets, mental retardation, or concomitant medical disorders were excluded. INTERVENTION(S) Additional data (type of schooling, foreign language learning, and bilingualism) were obtained with questionnaires. German-speaking children with foreign tuition language (English and/or French) at school were enrolled for further testing. MAIN OUTCOME MEASURE(S) General patient data, auditory data, and foreign language data from both questionnaires and tests were collected and analyzed. RESULTS Thirty seven out of 43 questionnaires (86%) were completed. Sixteen (43%) were in mainstream education. Twenty-seven CI users (73%) have foreign language learning at school. Fifteen of these were in mainstream education (55%), others in special schooling. From 10 CI users without foreign language learning, one CI user was in mainstream education (10%) and nine patients (90%) were in special schooling. Eleven German-speaking CI users were further tested in English and six additionally in French. For reading skills, the school objectives for English were reached in 7 of 11 pupils (64%) and for French in 3 of 6 pupils (50%). For listening skills, 3 of 11 pupils (27%) reached the school norm in English and none in French. CONCLUSIONS Almost 75% of our CI users learn foreign language(s) at school. A small majority of the tested CI users reached the current school norm for in English and French in reading skills, whereas for hearing skills most of them were not able to reach the norm.
Efficacy of locally-delivered 1% metformin gel in the treatment of intrabony defects in patients with chronic periodontitis: a randomized, controlled clinical trial.

OBJECTIVE Metformin (MF), used for the treatment of type 2 diabetes mellitus, has shown to possess properties favoring osteoblastic proliferation. The present study was designed to investigate the effectiveness of MF 1% gel as an adjunct to scaling and root planing in the treatment of intrabony defects in patients with chronic periodontitis. METHODS The study comprised 65 individuals divided into two groups: 1% MF with SRP and placebo gel with SRP. Clinical parameters were evaluated at baseline, 3 months, and 6 months; they included plaque index, modified sulcus bleeding index, probing depth (PD), and clinical attachment level (CAL). Intrabony defect depth (IBD) was evaluated at the end of 6 months using computer-aided software. RESULTS The mean PD reduction, CAL gain, and IBD depth reduction were found to be greater in the MF group than in the placebo group at all visits. The percentage of defect depth reduction was significantly greater in the MF group (26.8 ± 5.52%) than in the placebo sites (4.79 ± 2.30%, P < 0.001). CONCLUSION One percent MF was found to significantly improve clinical and radiographic parameters in intrabony defects in patients with chronic periodontitis.
PROP Nontaster Women Lose More Weight Following a Low‐Carbohydrate Versus a Low‐Fat Diet in a Randomized Controlled Trial

Taste blindness to 6‐n‐propylthiouracil (PROP) associates with increased fat preference and intake. No studies have matched a diet to a woman's PROP phenotype to improve weight loss. This study investigated (1) whether PROP nontaster (NT) women would lose more weight following a low‐carbohydrate (LC) diet than a low‐fat (LF) diet, and (2) whether PROP supertaster (ST) women would lose more weight following a LF diet than a LC diet.
Machine Intelligence on Resource-Constrained IoT Devices

Despite their remarkable performance in various machine intelligence tasks, the computational intensity of Convolutional Neural Networks (CNNs) has hindered their widespread utilization in resource-constrained embedded and IoT systems. To address this problem, we present a framework for synthesis of efficient CNN inference software targeting mobile SoC platforms. We argue that thread granularity can substantially impact the performance and energy dissipation of the synthesized inference software, and demonstrate that launching the maximum number of logical threads, often promoted as a guiding principle by GPGPU practitioners, does not result in an efficient implementation for mobile SoCs. We hypothesize that the runtime of a CNN layer on a particular SoC platform can be accurately estimated as a linear function of its computational complexity, which may seem counter-intuitive, as modern mobile SoCs utilize a plethora of heterogeneous architectural features and dynamic resource management policies. Consequently, we develop a principled approach and a data-driven analytical model to optimize granularity of threads during CNN software synthesis. Experimental results with several modern CNNs mapped to a commodity Android smartphone with a Snapdragon SoC show up to 2.37X speedup in application runtime, and up to 1.9X improvement in its energy dissipation compared to existing approaches.
Direct Laser Interference Patterning of CoCr Alloy Surfaces to Control Endothelial Cell and Platelet Response for Cardiovascular Applications

The main drawbacks of cardiovascular bare‐metal stents (BMS) are in‐stent restenosis and stent thrombosis as a result of an incomplete endothelialization after stent implantation. Nano‐ and microscale modification of implant surfaces is a strategy to recover the functionality of the artery by stimulating and guiding molecular and biological processes at the implant/tissue interface. In this study, cobalt‐chromium (CoCr) alloy surfaces are modified via direct laser interference patterning (DLIP) in order to create linear patterning onto CoCr surfaces with different periodicities (≈3, 10, 20, and 32 µm) and depths (≈20 and 800 nm). Changes in surface topography, chemistry, and wettability are thoroughly characterized before and after modification. Human umbilical vein endothelial cells' adhesion and spreading are similar for all patterned and plain CoCr surfaces. Moreover, high‐depth series induce cell elongation, alignment, and migration along the patterned lines. Platelet adhesion and aggregation decrease in all patterned surfaces compared to CoCr control, which is associated with changes in wettability and oxide layer characteristics. Cellular studies provide evidence of the potential of DLIP topographies to foster endothelialization without enhancement of platelet adhesion, which will be of high importance when designing new BMS in the future.