Miracle-dz/UniFAR-FTUs huggingface dataset:
PCR based high risk HPV testing is superior to neural network based screening for predicting incident CIN III in women with normal cytology and borderline changes

Background/Aims—To improve the accuracy of conventional cytology in cervical cancer screening, high risk human papillomavirus (HPV) testing and neural network based screening have been developed. This study assessed the power of both techniques to detect women at risk of developing incident CIN III; that is, CIN III detected during the follow up of women with normal cytology and borderline nuclear changes. Methods—A cohort of 2250 women, 34–54 years of age, who attended population based cervical cancer screening from 1988 to 1991 and had normal smears or borderline nuclear changes was followed. All smears were tested for high risk HPV and the smears were rescreened using neural network based screening. The value of neural network based screening for predicting incident CIN III during a mean follow up period of 6.4 years was compared with that of high risk HPV testing. In addition, morphological markers presumed to be related to HPV were correlated with HPV status. Results—Thirteen (0.6%) women had incident CIN III. Both high risk HPV positivity and abnormal cytology were associated with an increased risk for incident CIN III (odds ratio, 240 and 22, respectively) and high risk HPV positivity was associated with abnormal cytology. The sensitivity of high risk HPV testing for predicting incident CIN III was much higher than that of neural network based screening (92% and 46%, respectively). None of the morphological markers assessed, including koilocytosis, was correlated with high risk HPV status. Conclusion—High risk HPV testing is superior to neural network based screening in identifying women at risk of developing CIN III. For women with normal cytology and borderline changes and a negative high risk HPV test, the screening interval can be considerably prolonged.
Physiological and agonistic behavioural response of Procambarus clarkii to an acoustic stimulus

SUMMARY This study examined the effects of an acoustic stimulus on the haemolymph and agonistic behaviour of the red swamp crayfish, Procambarus clarkii. The experiment was conducted in a tank equipped with a video recording system using six groups (three control and three test groups) of five adult crayfish (30 specimens in total). After 1 h of habituation, the behaviour of the crayfish was monitored for 2 h. During the second hour, the animals in the test groups were exposed to a linear sweep (frequency range 0.1–25 kHz; peak amplitude 148 dBrms re. 1 μPa at 12 kHz) acoustic stimulus for 30 min. Exposure to the noise produced significant variations in haemato-immunological parameters as well as a reduction in agonistic behaviour.
Role of the Gulf Stream and Kuroshio–Oyashio Systems in Large-Scale Atmosphere–Ocean Interaction: A Review

Abstract Ocean–atmosphere interaction over the Northern Hemisphere western boundary current (WBC) regions (i.e., the Gulf Stream, Kuroshio, Oyashio, and their extensions) is reviewed with an emphasis on their role in basin-scale climate variability. SST anomalies exhibit considerable variance on interannual to decadal time scales in these regions. Low-frequency SST variability is primarily driven by basin-scale wind stress curl variability via the oceanic Rossby wave adjustment of the gyre-scale circulation that modulates the latitude and strength of the WBC-related oceanic fronts. Rectification of the variability by mesoscale eddies, reemergence of the anomalies from the preceding winter, and tropical remote forcing also play important roles in driving and maintaining the low-frequency variability in these regions. In the Gulf Stream region, interaction with the deep western boundary current also likely influences the low-frequency variability. Surface heat fluxes damp the low-frequency SST anomalies ove.
A scalable active framework for region annotation in 3D shape collections

Large repositories of 3D shapes provide valuable input for data-driven analysis and modeling tools. They are especially powerful once annotated with semantic information such as salient regions and functional parts. We propose a novel active learning method capable of enriching massive geometric datasets with accurate semantic region annotations. Given a shape collection and a user-specified region label our goal is to correctly demarcate the corresponding regions with minimal manual work. Our active framework achieves this goal by cycling between manually annotating the regions, automatically propagating these annotations across the rest of the shapes, manually verifying both human and automatic annotations, and learning from the verification results to improve the automatic propagation algorithm. We use a unified utility function that explicitly models the time cost of human input across all steps of our method. This allows us to jointly optimize for the set of models to annotate and for the set of models to verify based on the predicted impact of these actions on the human efficiency. We demonstrate that incorporating verification of all produced labelings within this unified objective improves both accuracy and efficiency of the active learning procedure. We automatically propagate human labels across a dynamic shape network using a conditional random field (CRF) framework, taking advantage of global shape-to-shape similarities, local feature similarities, and point-to-point correspondences. By combining these diverse cues we achieve higher accuracy than existing alternatives. We validate our framework on existing benchmarks demonstrating it to be significantly more efficient at using human input compared to previous techniques. We further validate its efficiency and robustness by annotating a massive shape dataset, labeling over 93,000 shape parts, across multiple model classes, and providing a labeled part collection more than one order of magnitude larger than existing ones.
Is the Pain Visual Analogue Scale Linear and Responsive to Change? An Exploration Using Rasch Analysis

Objectives Pain visual analogue scales (VAS) are commonly used in clinical trials and are often treated as an interval level scale without evidence that this is appropriate. This paper examines the internal construct validity and responsiveness of the pain VAS using Rasch analysis. Methods Patients (n = 221, mean age 67, 58% female) with chronic stable joint pain (hip 40% or knee 60%) of mechanical origin waiting for joint replacement were included. Pain was scored on seven daily VASs. Rasch analysis was used to examine fit to the Rasch model. Responsiveness (Standardized Response Means, SRM) was examined on the raw ordinal data and the interval data generated from the Rasch analysis. Results Baseline pain VAS scores fitted the Rasch model, although 15 aberrant cases impacted on unidimensionality. There was some local dependency between items but this did not significantly affect the person estimates of pain. Daily pain (item difficulty) was stable, suggesting that single measures can be used. Overall, the SRMs derived from ordinal data overestimated the true responsiveness by 59%. Changes over time at the lower and higher end of the scale were represented by large jumps in interval equivalent data points; in the middle of the scale the reverse was seen. Conclusions The pain VAS is a valid tool for measuring pain at one point in time. However, the pain VAS does not behave linearly and SRMs vary along the trait of pain. Consequently, Minimum Clinically Important Differences using raw data, or change scores in general, are invalid as these will either under- or overestimate true change; raw pain VAS data should not be used as a primary outcome measure or to inform parametric-based Randomised Controlled Trial power calculations in research studies; and Rasch analysis should be used to convert ordinal data to interval data prior to data interpretation.
Selective Spectrophotometric and Spectrofluorometric Methods for the Determination of Amantadine Hydrochloride in Capsules and Plasma via Derivatization with 1,2-Naphthoquinone-4-sulphonate

New selective and sensitive spectrophotometric and spectrofluorometric methods have been developed and validated for the determination of amantadine hydrochloride (AMD) in capsules and plasma. The methods were based on the condensation of AMD with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium to form an orange-colored product. The spectrophotometric method involved the measurement of the colored product at 460 nm. The spectrofluorometric method involved the reduction of the product with potassium borohydride, and the subsequent measurement of the formed fluorescent reduced AMD-NQS product at 382 nm after excitation at 293 nm. The variables that affected the reaction were carefully studied and optimized. Under the optimum conditions, linear relationships with good correlation coefficients (0.9972–0.9974) and low LOD (1.39 and 0.013 μg mL−1) were obtained in the ranges of 5–80 and 0.05–10 μg mL−1 for the spectrophotometric and spectrofluorometric methods, respectively. The precisions of the methods were satisfactory; RSD ≤2.04%. Both methods were successfully applied to the determination of AMD in capsules. As its higher sensitivity, the spectrofluorometric method was applied to the determination of AMD in plasma; the recovery was 96.3–101.2 ± 0.57–4.2%. The results obtained by the proposed methods were comparable with those obtained by the official method
Challenges of Assessing Maltreated Children Coming into Foster Care

Children who have experienced early adversity have been known to be at risk of developing cognitive, attachment, and mental health problems; therefore, it is crucial that children entering foster care can be properly assessed as early as possible. There are known difficulties in assessing children in foster care, for example, in finding a reliable informant. An ongoing randomised controlled trial in Glasgow, Scotland, recruiting infants entering foster care, provides a unique opportunity to explore some of the issues which need to be considered when assessing these children. The assessment data of 70 infants entering care is described while exploring the reliability of foster carers as informants and the importance of infant engagement with tasks. This group of infants was shown to be having more problems than children from the general population. While correlations were found between a carer's level of concern about a child and the severity of a child's problem, there were still a number of children displaying worrying problem scores whom foster carers did not report concern. The child's engagement in the cognitive task showed associations with the child's attainment on the task. Findings emphasise the importance of a holistic assessment for these children and all should be considered as potential cases with Maltreatment-Associated Psychiatric Problems (MAPP).
Enhancement of quinone redox cycling by ascorbate induces a caspase-3 independent cell death in human leukaemia cells. An in vitro comparative study

Since the higher redox potential of quinone molecules has been correlated with enhanced cellular deleterious effects, we studied the ability of the association of ascorbate with several quinones derivatives (having different redox potentials) to cause cell death in K562 human leukaemia cell line. The rationale is that the reduction of quinone by ascorbate should be dependent of the quinone half-redox potential thus determining if reactive oxygen species (ROS) are formed or not, leading ultimately to cell death or cell survival. Among different ROS that may be formed during redox cycling between ascorbate and the quinone, the use of different antioxidant compounds (mannitol, desferal, N-acetylcysteine, catalase and superoxide dismutase) led to support H2O2 as the main oxidizing agent. We observed that standard redox potentials, oxygen uptake, free ascorbyl radical formation and cell survival were linked. The oxidative stress induced by the mixture of ascorbate and the different quinones decreases cellular contents of ATP and GSH while caspase-3-like activity remains unchanged. Again, we observed that quinones having higher values of half-redox potential provoke a severe depletion of ATP and GSH when they were associated with ascorbate. Such a drop in ATP content may explain the lack of activation of caspase-3. In conclusion, our results indicate that the cytotoxicity of the association quinone/ascorbate on K562 cancer cells may be predicted on the basis of half-redox potentials of quinones.
The Bifunctional Entamoeba histolytica Alcohol Dehydrogenase 2 (EhADH2) Protein Is Necessary for Amebic Growth and Survival and Requires an Intact C-terminal Domain for Both Alcohol Dehydrogenase and Acetaldehyde Dehydrogenase Activity*

The intestinal protozoan pathogen Entamoeba histolytica lacks mitochondria and derives energy from the fermentation of glucose to ethanol with pyruvate, acetyl enzyme Co-A, and acetaldehyde as intermediates. A key enzyme in this pathway may be the 97-kDa bifunctional E. histolytica alcohol dehydrogenase 2 (EhADH2), which possesses both alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase activity (ALDH). EhADH2 appears to be a fusion protein, with separate N-terminal ALDH and C-terminal ADH domains. Here, we demonstrate that EhADH2 expression is required forE. histolytica growth and survival. We find that a mutant EhADH2 enzyme containing the C-terminal 453 amino acids of EhADH2 has ADH activity but lacks ALDH activity. However, a mutant consisting of the N-terminal half of EhADH2 possessed no ADH or ALDH activity. Alteration of a single histidine to arginine in the putative active site of the ADH domain eliminates both ADH and ALDH activity, and this mutant EhADH2 can serve as a dominant negative, eliminating both ADH and ALDH activity when co-expressed with wild-type EhADH2 inEscherichia coli. These data indicate that EhADH2 enzyme is required for E. histolytica growth and survival and that the C-terminal ADH domain of the enzyme functions as a separate entity. However, ALDH activity requires residues in both the N- and C-terminal halves of the molecule.
Effects of the molecular weight of hyaluronic acid and its action mechanisms on experimental joint pain in rats.

OBJECTIVES--It has been shown previously that hyaluronic acid (HA) has an analgesic action on bradykinin induced pain in the knee joints of rats. This study further clarifies the effects of the molecular weight of HA and its mechanism of action in the same model using HA of molecular weight 800 to 2.3 x 10(6) daltons and a bradykinin antagonist. METHODS--Bradykinin and the test HA preparations were given to rats by intra-articular injection, and the severity of pain was evaluated by a change in the walking behaviour. RESULTS--HA with a molecular weight greater than 40 kilodaltons produces analgesic effects with a simultaneous or earlier injection. The ID50 values of HA with molecular weight 40, 310, 860, and 2300 kilodaltons were greater than 2.5, 0.6, 0.07, and 0.06 mg/joint respectively. The duration of the analgesic effect of 860 and 2300 kilodalton HA was 72 hours at 10 mg/ml, whereas that of 310 kilodalton HA was short, being undetectable after 24 hours. The analgesic action of HA of 860 kilodaltons was not changed by pretreatment with four saccharide HA and inhibited by pretreatment with HA larger than six to eight saccharides, capable of binding to HA receptors. Further, HA did not interfere with the analgesic action of the bradykinin antagonist, indicating that HA does not directly bind with bradykinin receptors. CONCLUSIONS--HA with a molecular weight of greater than 40 kilodaltons produced an analgesic effect, and HA of 860 and 2300 kilodaltons produced high and long-lasting analgesia. These effects of HA appear to be caused by the interaction between HA and HA receptors.
Modeling and estimation of mutual coupling in a uniform linear array of dipoles

The mutual coupling in a uniform linear array (ULA) of dipoles is calculated using basic electromagnetic concepts. Since the coupling often is unknown and needs to be estimated, a simpler model is proposed based on the electromagnetic analysis. The parameterization of this model is shown to be locally unambiguous. A necessary condition for the joint solution of directions and coupling parameters to be unique is also derived. Finally, the directions and coupling parameters are estimated using a maximum likelihood method. It is found that the simpler coupling model with just a few parameters well describes the full electromagnetic model.
Spread and prevention of some common viral infections in community facilities and domestic homes

Viruses are probably the most common cause of infectious disease acquired within indoor environments and have considerable impact on human health, ranging from severe life-threatening illnesses to relatively mild and self-limiting or asymptomatic diseases. In particular, viruses causing gastrointestinal and respiratory diseases spread rapidly in the community and cause considerable morbidity. Increasing numbers of people who have impaired immunity, for whom the consequences of infection can be much more serious, are now cared for in "out of hospital" settings. This review examines the dispersal, persistence and control of some common viruses in the domestic home and in community facilities. There is growing evidence that person-to-person transmission via the hands and contaminated fomites plays a key role in the spread of viral infections and there is a need for wider understanding of the potential for contaminated surfaces to act as unidentified vectors of pathogens in the transmission cycle. Intervention studies have shown that improved standards of education, personal hygiene (particularly handwashing) and targeted environmental hygiene have considerable impact in the control and prevention of infectious organisms.
A High‐Optical Quality Supramolecular Assembly for Third‐Order Integrated Nonlinear Optics

A high-quality organic thin film for integrated nonlinear optics is produced by molecular beam deposition of a compact molecule that has a high specific third-order polarizability. The resulting dense, homogenous, amorphous supramolecular assembly with a large third-order nonlinearity and high-optical quality enables novel applications in integrated nonlinear optics.
Homogeneous varieties---zero-cycles of degree one versus rational points

Examples of projective homogeneous varieties over the field of Laurent series over p-adic fields which admit zero cycles of degree one and which do not have rational points are constructed. Let k be a field and X a quasi-projective variety over k. Let Z0(X) denote the group of zero cycles on X and deg : Z0(X) → Z the degree homomorphism which associates to a closed point x of X, the degree [k(x) : k] of its residue field. We study the question: for what classes X of varieties (respectively, what classes of fields k) is it true that for X ∈ X, if X admits a 0-cycle of degree one, then X has a rational point. If X is a curve of genus zero or one over any field k, then X has a rational point once it admits a zero cycles of degree one. However, the question is more reasonable to ask for classes of rational varieties or homogeneous varieties. In the setting of rational varieties, there are examples, due to Colliot-Thelene and Coray [CTC] of conic bundles over the projective line over a p-adic field with a zero cycle of degree one, which have no rational points. We shall list from literature some questions in this direction for homogeneous varieties. Q(HPr) (Veisfeiler)[V] Let X be a projective homogeneous variety under a connected linear algebraic group defined over a field k. If X has a zero cycle of degree one, does X have a rational point?
Systemic and Local Responses to Repeated HL Stress-Induced Retrograde Signaling in Arabidopsis

Chloroplasts of leaves under high light stress initiate signals to the nuclei of both exposed and distal leaves in order to acclimate against the potential threat of oxidative damage: a process known as high light systemic acquired acclimation (HL SAA). This study explores the nature of HL SAA, synergistic interactions with other environmental stresses, and the impact of repeated HL stress on the acclimation response of exposed and distal leaves. This necessitated the development of novel experimental systems to investigate the initiation, perception, and response to HL SAA. These systems were used to investigate the HL SAA response by monitoring the induction of mRNA in distal leaves not exposed to the HL stress. Acclimation to HL is induced within minutes and the response is proportionally dependent on the quality and quantity of light. HL SAA treatments in conjunction with variations in temperature and humidity reveal HL SAA is influenced by fluctuations in humidity. These treatments also result in changes in auxin accumulation and auxin-responsive genes. A key question in retrograde signaling is the extent to which transient changes in light intensity result in a “memory” of the event leading to acclimation responses. Repeated exposure to short term HL resulted in acclimation of the exposed tissue and that of emerging and young leaves (but not older leaves) to HL and oxidative stress.
Shear and extensional flow of reinforced plastics in injection molding. II. Effects of die angle and bore diameter on entry pressure with bulk molding compound

Orifice flow measurements have been performed at high strain rates on bulk molding compound (BMC) using convergent dies. A range of die angles and bore diameters was examined. Two models for flow behavior have been compared. It appears that, with BMC, there is a transition from constrained convergence to convergence with recirculation at a die semiangle of approximately 56 degrees.
Rapid hemostasis in a sheep model using particles that propel thrombin and tranexamic acid

Bleeding during endoscopic sinus surgery and open surgeries can easily obstruct the surgeons' field of view and increase morbidity and risk of intraoperative complications. Intraoperative bleeding could potentially be addressed by a hemostatic agent that safely disperses itself through the escaping blood. We tested the safety and efficacy of a self‐propelling formulation of thrombin and tranexamic acid (SPTT) in stopping bleeding in a paranasal sinus injury and in an open surgical carotid injury sheep model.