Miracle-dz/UniFAR-FTUs huggingface dataset:
A single nucleotide substitution in the internal ribosome entry site of foot-and-mouth disease virus leads to enhanced cap-independent translation in vivo

Mutants of foot-and-mouth disease virus (FMDV) with altered biological properties can be selected during the course of persistent infection of BHK-21 cells with FMDV C-S8c1 (J. C. de la Torre, E. Martínez-Salas, J. Díez, A. Villaverde, F. Gebauer, E. Rocha, M. Dávila, and E. Domingo, J. Virol. 62:2050-2058, 1988). Two nucleotide substitutions, U to C at position -376 and A to G at position -15, (counting as +1 the A of the first functional AUG), were fixed within the internal ribosome entry site (IRES) of R100, the virus rescued after 100 passages of the carrier BHK-21 cells. IRES-directed cap-independent protein synthesis was quantitated by using bicistronic constructs of the form chloramphenicol acetyltransferase gene-IRES-luciferase gene. The IRES from R100 was 1.5- to 5-fold more active than that of C-S8c1 in directing cap-independent luciferase synthesis. This enhanced translational activity was observed when the RNAs were transcribed either in the nucleus or in the cytoplasm by a weak or a strong promoter, respectively. C-S8c1 and R100 IRES elements were functional in both FMDV-sensitive and FMDV-resistant cells (including persistently infected R cells), indicating that factors mediating cap-independent protein synthesis are not limited in any of the analyzed cell lines. Constructs in which each of the two mutations in the R100 IRES were analyzed separately indicate that the transition at position -376 is responsible for the enhanced activity of the R100 IRES. By estimating the effect that an increase in the initial translation efficiency may have on subsequent RNA replication steps, we suggest that the modifications in the IRES elements can account for the previously described hypervirulence of FMDV R100 for BHK-21 cells. The results show that a single point mutation in an IRES element of a picornavirus can cause an increase in translation efficiency.
BendDesk: dragging across the curve

We present BendDesk, a hybrid interactive desk system that combines a horizontal and a vertical interactive surface via a curve. The system provides seamless touch input across its entire area. We explain scalable algorithms that provide graphical output and multi-touch input on a curved surface. In three tasks we investigate the performance of dragging gestures across the curve, as well as the virtual aiming at targets. Our main findings are: 1) Dragging across a curve is significantly slower than on flat surfaces. 2) The smaller the entrance angle when dragging across the curve, the longer the average trajectory and the higher the variance of trajectories across users. 3) The curved shape of the system impairs virtual aiming at targets.
Rethinking the duration requirement for generalized anxiety disorder: evidence from the National Comorbidity Survey Replication

Background. The proposed revisions of the ICD and DSM diagnostic systems have led to increased interest in evaluation of diagnostic criteria. This report focuses on the DSM-IV requirement that episodes of generalized anxiety disorder (GAD) must persist for at least 6 months. Community epidemiological data are used to study the implications of changing this requirement in the range 1–12 months for estimates of prevalence, onset, course, impairment, co-morbidity, associations with parental GAD, and sociodemographic correlates. Method. Data come from the US National Comorbidity Survey Replication (NCS-R), a US household survey carried out during 2001–2003. Version 3.0 of the WHO Composite International Diagnostic Interview (WMH-CIDI) was used to assess DSM-IV anxiety disorders, mood disorders, substance disorders, and impulse-control disorders. Results. Lifetime, 12-month, and 30-day prevalence estimates of DSM-IV GAD changed from 6·1%, 2·9%, and 1·8% to 4·2–12·7%, 2·2–5·5%, and 1·6–2·6% when the duration requirement was changed from 6 months to 1–12 months. Cases with episodes of 1–5 months did not differ greatly from those with episodes of [ges ]6 months in onset, persistence, impairment, co-morbidity, parental GAD, or sociodemographic correlates. Conclusions. A large number of people suffer from a GAD-like syndrome with episodes of <6 months duration. Little basis for excluding these people from a diagnosis is found in the associations examined here.
The effects of shadow removal on across-date settlement type classification of QuickBird images

QuickBird imagery acquired on separate dates may have significant differences in viewing- and illumination geometries, which can negatively impact across-date settlement type classification accuracy. The effect of cast shadows on classification accuracy is specifically investigated in this study by performing shadow removal, before the calculation of texture features which are then used as classifier inputs. It is shown that popular texture features are sensitive to differences in shadow profiles, and that across-date classification accuracy can be improved with shadow removal. The strong relationship between the best shadow detection thresh-old and the best settlement classification accuracy is indicated for the study data set. A refined shadow correction algorithm is proposed, and demonstrated to be more effective than basic shadow correction methods.
Instructional recommendations for the teaching of writing

Clearly, writing is an essential life, work, and educational skill, and far too many students without essential writing skills are receiving high school diplomas. These realities put many recent high school graduates at a disadvantage in personal, professional, and educational arenas. Writing skills are simply too important to be ignored. This reality begs an important question: Which instructional approaches are likely to lead to improved student writing? For the purposes of this paper, research will center mainly on controlled studies, though much insight also stands to be gained from studies of what writers and teachers of writing have to say about their own processes and experiences. This open access graduate research paper is available at UNI ScholarWorks: http://scholarworks.uni. edu/grp/14 Writing recommendations 1 Running Head: WRITING RECOMMENDATIONS Instructional Recommendations for the Teaching of Writing Submitted In Partial Fulfillment of the Requirements for the Degree Master of Arts in Education Bridgette Wagoner University of Northern Iowa September 2007 Writing recommendations 2
Differences in Word Recognition Based on Approach to Reading Instruction.

Cette etude vise a reperer des differences dans la competence a reconnaitre des mots chez des eleves qui ont appris a lire selon differentes methodes. 54 eleves participent a l'etude, 27 provenant de classes se servant de la methode globale et 27 de classes se servant de la methode syllabique. Le niveau en lecture est determine par le module test de comprehension en lecture du Gates-MacGinitie (MacGinitie & MacGinitie, 1992). Le critere de participation a l'etude est fixe au 25e percentile ou plus, les eleves ayant moins de 2.2 et trouvant les textes choisis trop difficiles n'etant pas retenus. A la fin de la 2eme annee primaire, les eleves sont soumis aux tâches suivantes: (a) identification de mots isoles et en contexte; (b) substitution de grapheme dans un texte; et (c) un exercice de phrases a completer. L'exercice de completement est la seule epreuve pour laquelle on mesure une difference significative en faveur du groupe Methode globale, aucune autre difference significative n'existe dans les autres tâches d'identification, ce qui suggere que l'approche pedagogique a peu d'effets sur les strategies de reconnaissance du vocabulaire.
Influence of Widening Electrode Separation on Current Steering Performance

Objectives: Current steering between adjacent electrodes makes it possible to create more spectral channels than the number of electrodes in an electrode array. With current steering on nonadjacent electrodes, called “spanning,” it could be possible to bridge a defective electrode contact or potentially reduce the number of electrode contacts for the same level of access to the auditory nerve. This study investigates the effectiveness of spanning in terms of the number of intermediate pitches, loudness effects, and linearity of the current weighting coefficient (&agr;) with respect to the perceived pitch. Design: Twelve postlingually deafened users of the HiRes90K cochlear implant with HiFocus1j electrode were randomly selected to participate in this study. Electrode contacts were selected at two locations in the cochlea, as determined on multislice computed tomography: 180° (basal) and 360° (apical) from the round window. For both cochlear locations, three psychophysical experiments were performed using simultaneous stimulation of electrode contacts. An adaptive staircase-based procedure was used. The number of intermediate pitches was assessed with a three-alternative forced choice (3AFC) pitch discrimination task, and the extent of current adjustment required when varying the current weighting coefficient (&agr;) was determined with loudness balancing (2AFC). Finally, the pitch of a spanned channel was matched with the pitch of an intermediate physical electrode in a 2AFC procedure to assess the place of the spanned channel on the electrode array. Results: Spanning required significantly more current compensation to maintain equal loudness than current steering between adjacent electrode contacts. A significant decrease of discriminable intermediate pitches occurred with spanning in comparison with current steering between adjacent electrode contacts. No significant difference was found between the pitch-matched current steering coefficient and the theoretical coefficient corresponding a priori with the intermediate physical electrode. No significant difference was found between the data from the apical and the basal sections of the electrode array. Conclusions: Spanning over wider electrode distance is feasible. With increasing electrode spanning distance, more current compensation is needed to maintain equal loudness, and a gradual deterioration in the just noticeable difference for pitch is observed. However, the pitch progression is linear. For a spanned signal with equal proportions of current delivered to both electrodes, pitch is equivalent to that produced by an intermediate physical electrode.
Five General Properties of Resolution

This paper presents five properties that are true of the resolution of (geographic) data, and discusses their implications for Geographic Information Science (GIScience). It argues that resolution is (i) always present in data, (ii) representation-dependent, (iii) positively correlated with accuracy, (iv) positively correlated with data volume, and (v) more specific than granularity. These statements are brought forward with the intent of stimulating discussions, and should be seen as provisional, not definitive, much less exhaustive regarding possible laws pertaining to resolution.
Inhibitors of hydroperoxide metabolism enhance ascorbate-induced cytotoxicity

Abstract Pharmacological ascorbate, via its oxidation, has been proposed as a pro-drug for the delivery of H2O2 to tumors. Pharmacological ascorbate decreases clonogenic survival of pancreatic cancer cells, which can be reversed by treatment with scavengers of H2O2. The goal of this study was to determine if inhibitors of intracellular hydroperoxide detoxification could enhance the cytotoxic effects of ascorbate. Human pancreatic cancer cells were treated with ascorbate alone or in combination with inhibitors of hydroperoxide removal including the glutathione disulfide reductase inhibitor 1,3 bis (2-chloroethyl)-1-nitrosurea (BCNU), siRNA targeted to glutathione disulfide reductase (siGR), and 2-deoxy-D-glucose (2DG), which inhibits glucose metabolism. Changes in the intracellular concentration of H2O2 were determined by analysis of the rate of aminotriazole-mediated inactivation of endogenous catalase activity. Pharmacological ascorbate increased intracellular H2O2 and depleted intracellular glutathione. When inhibitors of H2O2 metabolism were combined with pharmacological ascorbate the increase in intracellular H2O2 was amplified and cytotoxicity was enhanced. We conclude that inclusion of agents that inhibit cellular peroxide removal produced by pharmacological ascorbate leads to changes in the intracellular redox state resulting in enhanced cytotoxicity.
Enhanced Tumour Uptake of Doxorubicin Loaded Poly(butyl cyanoacrylate) Nanoparticles in Mice Bearing Dalton's Lymphoma Tumour

The objective of this study is to enhance the delivery of Doxorubicin hydrochloride to Dalton's lymphoma solid tumour through poly(butyl cyanoacrylate) (PBC) nanoparticles. Doxorubicin loaded PBC (DPBC) nanoparticles were prepared by emulsion polymerization and characterized by particle size analysis, zeta potential and scanning electron microscopy. Doxorubicin HCl (Dox) and DPBC nanoparticles were radiolabeled with 99mTc by reduction method using stannous chloride and optimized the labeling parameters to obtain high labeling efficiency. The in vitro stability of 99mTc-labeled complexes was determined by DTPA and cysteine challenge test. The labeled complexes showed very low transchelation and high in vitro and serum stability. 99mTc labeled complexes of Dox and DPBC nanoparticles were administered subcutaneously below the Dalton's lymphoma tumour and biodistribution was studied. The distribution of DPBC nanoparticles to the blood, heart and organs of RES such as liver, lung and spleen was biphasic with a rapid initial distribution, followed by a significant decrease later at 6 h post-injection. The distribution of Dox to tissues was very low initially and increased significantly at 6 h post-injection indicating its accumulation at the injection site for a longer time. The concentration of DPBC nanoparticles was also found high in tissues at 6 h post-injection indicating their accumulation at the subcutaneous site and consequent disposition to tissues with time. A significantly high tumour uptake of DPBC nanoparticles (∼13 fold higher at 48 h post-injection) (P <0.001) was found compared to free Dox. The tumour concentrations of both Dox and DPBC nanoparticles increased with time indicating their slow penetration from the injection site into tumour. The concentration of DPBC nanoparticles in the femur bone in the tumour region was also significantly higher (P <0.001) than free Dox and increased with time. The study signifies the advantage of delivering Dox to Dalton's lymphoma through PBC nanoparticles by facilitating enhanced tumour uptake and prolonged tumour retention, which are expected to lead to greater therapeutic effect in the form of tumour regression.
Hippuric Acid in 24-Hour Urine Is a Potential Biomarker for Fruit and Vegetable Consumption in Healthy Children and Adolescents 1 , 2

An objective noninvasive biomarker for fruit and vegetable (FV) consumption would help to more reliably characterize the relationship between FV intake and health status in observational studies. Because increases in urinary hippuric acid (HA) were observed after consumption of several FV varieties, we aimed to investigate whether 24-h urinary HAmay represent a potential biomarker for FV consumption in children and adolescents. The association of FV and juice (FVJ) intake calculated from 3-d weighed dietary records with 24-h urinary HA was analyzed in 240 healthy children and adolescents and compared with associations of the established biomarkers urinary nitrogen (uN) and potassium (uK) with protein and potassium intake, respectively. Spearman correlation coefficients (r) and cross-classifications were calculated for all dietbiomarker associations. Potential confounders for the HA-FVJ association were examined in linear regression models. In children, correlations of HA with FVJ (r = 0.62), uN with protein (r = 0.64), and potassium intake with uK (r = 0.65) were comparable. In adolescents, the HA-FVJ association was weaker (r = 0.41) compared with the biomarkers uN (r = 0.60) and uK (r= 0.58) (allP, 0.0001). Cross-classification into the same/adjacent quartile by dietary and urinary datawere.85% for all analyzed comparisons except for a 75% classification agreement between HA and FVJ in adolescents. Unadjusted and adjusted linear regression models indicated significant (P , 0.0001) HA-FVJ associations in both age groups. FVJ explained more of the variability in HA excretion in children (R = 0.38) than in adolescents (R = 0.22). Our findings in children showing HA-FVJ associations comparable to those for well-established biomarkers with their respective dietary intakes suggest that HA may represent a useful biomarker for FVJ. J. Nutr. doi: 10.3945/jn.112. 159319.
Improving Intrinsic Motivation among Sophomore Electrical Engineering Students by an Introductory Project

800x600 In 2011, the Technion – Israel Institute of Technology decided to open a new elective course designed for sophomore electrical engineering students. The course was devised to expose students to the discipline of electrical engineering and improve their motivation. The core of the course was a team-based design project of a window cleaning robot. The present mixed-method study indicates a significant improvement in intrinsic motivation of the students who took the course. Normal 0 21 false false false DE X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Normale Tabelle"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:. 0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";}
First Report of Tripled Retromolar Foramina

The retromolar foramen (RMF) is the bony entrance of the retromolar canal, through which a neurovascular bundle runs. It is essential to locate such anatomic variants in a patient in order to avoid complications in surgery, implant placement, or anesthesia of the area. To our knowledge, there has only been one case report of supernumerary RMF, which reported one bilateral doubled retromolar foramina and one doubled left RMF. We present an extremely rare case in which a right triple RMF was observed on cone beam computed tomography in a cadaver. Diameters of the RMF were 0.8 mm, 1.0 mm and 1.1 mm, respectively. Distances from the distal edge of the third molar were measured as 4.0 mm, 3.6 mm, and 0.5 mm, respectively. RMF was not found on the left side of the mandible.
Film, interrupted: investigating how mobile device notifications affect immersion during movies

Mobile devices are increasingly used while watching video, both as a secondary device and for dedicated viewing. However, devices frequently issue notifications that can interrupt viewing. This study investigated the effect of interruptions from notifications on viewer immersion. Participants watched 10 minutes of a movie without notifications, and 10 minutes while receiving message notifications. There were two participant groups: one watched video on a 30-inch monitor with messages sent to a separate smartphone; while another watched on a smartphone while also receiving messages on it. Viewer immersion was assessed after each condition via questionnaire. We also considered message response times. Results showed that immersion scores were lower when the video was interrupted with notifications, regardless of viewing device. Message response times were fastest when using the phone as a secondary device. Our results suggest that device-driven interruptions should be minimised for an immersive film experience.
Glycophorin A‐mediated haemolysis of normal human erythrocytes: evidence for antigen aggregation in the pathogenesis of immune haemolysis

Summary. The inexplicable severity of anti‐Pr autoimmune haemolytic anaemia led us to test the hypothesis that the haemolysis was primarily due to a change in the function of glycophorin A, on which the Pr antigen is located. The lectins Maclura pomifera and wheat germ agglutinin that bind to glycophorin A induced the haemolysis of normal erythrocytes in vitro. Lectin binding led to an increase in erythrocyte membrane permeability to sodium and potassium, the former resulting in an influx of water and subsequent haemolysis. The response was glycophorin A specific as Concanavalin A, which binds to band 3, did not cause haemolysis and peanut agglutinin only did so after removal of erythrocyte sialic acid. The lectin‐induced cation leak was not mediated by activation of cation channels as the inhibitors, tetrodotoxin, amiloride and 4,4′ diisothiocyanate stilbene 2,2′disulphonate, had no effect, suggesting that the haemolysis was due to exacerbation of the inherent cation permeability of the erythrocyte membrane. A human IgAK anti‐Pr autoantibody and a mouse anti‐human glycophorin A antibody increased erythrocyte permeability to sodium. The role of glycophorin A in stabilizing and, upon aggregation, destabilizing the phospholipid bilayer is discussed. Our findings may help explain the severity of anti‐Pr autoimmune haemolytic anaemia and other pathophysiological changes in human erythrocytes.
Multivariate Out-of-Sample Tests for Granger Causality

A time series is said to Granger cause another series if it has incremental predictive power when forecasting it. While Granger causality tests have been studied extensively in the univariate setting, much less is known for the multivariate case. Multivariate out-of-sample tests for Granger causality are proposed and their performance is measured by a simulation study. The results are graphically represented by size-power plots. It emerges that the multivariate regression test is the most powerful among the considered possibilities. As a real data application, it is investigated whether the consumer confidence index Granger causes retail sales in Germany, France, the Netherlands and Belgium.
Cytokines and Hormones That Contribute to the Positive Association between Fat and Bone

The positive association between body weight and bone density has been established in numerous laboratory and clinical studies. Apart from the direct effect of soft tissue mass on bone through skeletal loading, a number of cytokines and hormones contribute to the positive association between adipose and bone tissue, acting either locally in sites where cells of the two tissues are adjacent to each other or systemically through the circulation. The current review describes the effects of such local and systemic factors on bone physiology. One class of factors are the adipocyte-secreted peptides (adipokines), which affect bone turnover through a combination of direct effects in bone cells and indirect mechanisms mediated by the central nervous system. Another source of hormones that contribute to the coupling between fat and bone tissue are beta cells of the pancreas. Insulin, amylin, and preptin are co-secreted from pancreatic beta cells in response to increased glucose levels after feeding, and are also found in high circulating levels in obesity. A number of peptide hormones secreted from the gastrointestinal tract in response to feeding affect both fat and bone cells and thus can also act as mediators of the association between the two tissues. The current review focuses on results of laboratory studies investigating possible mechanism involved in the positive association between fat mass and bone mass.