Miracle-dz/UniFAR-FTUs huggingface dataset:
Adhesion- and Degranulation-Promoting Adapter Protein Promotes CD8 T Cell Differentiation and Resident Memory Formation and Function during an Acute Infection

During acute infections, naive Ag-specific CD8 T cells are activated and differentiate into effector T cells, most of which undergo contraction after pathogen clearance. A small population of CD8 T cells persists as memory to protect against future infections. We investigated the role of adhesion- and degranulation-promoting adapter protein (ADAP) in promoting CD8 T cell responses to a systemic infection. Naive Ag-specific CD8 T cells lacking ADAP exhibited a modest expansion defect early after Listeria monocytogenes or vesicular stomatitis virus infection but comparable cytolytic function at the peak of response. However, reduced numbers of ADAP-deficient CD8 T cells were present in the spleen after the peak of the response. ADAP deficiency resulted in a greater frequency of CD127+ CD8 memory precursors in secondary lymphoid organs during the contraction phase. Reduced numbers of ADAP-deficient killer cell lectin-like receptor G1− CD8 resident memory T (TRM) cell precursors were present in a variety of nonlymphoid tissues at the peak of the immune response, and consequently the total numbers of ADAP-deficient TRM cells were reduced at memory time points. TRM cells that did form in the absence of ADAP were defective in effector molecule expression. ADAP-deficient TRM cells exhibited impaired effector function after Ag rechallenge, correlating with defects in their ability to form T cell–APC conjugates. However, ADAP-deficient TRM cells responded to TGF-β signals and recruited circulating memory CD8 T cells. Thus, ADAP regulates CD8 T cell differentiation events following acute pathogen challenge that are critical for the formation and selected functions of TRM cells in nonlymphoid tissues.
A Local Clustering Algorithm for Massive Graphs and Its Application to Nearly Linear Time Graph Partitioning

We study the design of local algorithms for massive graphs. A local graph algorithm is one that finds a solution containing or near a given vertex without looking at the whole graph. We present a local clustering algorithm. Our algorithm finds a good cluster---a subset of vertices whose internal connections are significantly richer than its external connections---near a given vertex. The running time of our algorithm, when it finds a nonempty local cluster, is nearly linear in the size of the cluster it outputs. The running time of our algorithm also depends polylogarithmically on the size of the graph and polynomially on the conductance of the cluster it produces. Our clustering algorithm could be a useful primitive for handling massive graphs, such as social networks and web-graphs. As an application of this clustering algorithm, we present a partitioning algorithm that finds an approximate sparsest cut with nearly optimal balance. Our algorithm takes time nearly linear in the number edges of the graph.
Beliefs About Willpower Are Related to Therapy Adherence and Psychological Adjustment in Patients With Type 2 Diabetes

Research suggests that self-control, the ability to forego immediate needs for the sake of future rewards, promotes health behavior. The present study examined the role of beliefs about willpower as predictor of self-control in the context of diabetes. Seventy-nine type 2 diabetes patients reported their beliefs about willpower, therapy adherence (i.e., self-care activities, diet, exercise), and psychological adjustment (i.e., emotional distress, well-being, life quality). Endorsing the belief that willpower is a limited versus nonlimited resource was associated with lower therapy adherence and lower psychological adjustment. How people think about their willpower might be crucial for therapy success in diabetes patients.
A Breath of Fresh Air on the Mesenchyme: Impact of Impaired Mesenchymal Development on the Pathogenesis of Bronchopulmonary Dysplasia

The early mouse embryonic lung, with its robust and apparently reproducible branching pattern, has always fascinated developmental biologists. They have extensively used this embryonic organ to decipher the role of mammalian orthologs of Drosophila genes in controlling the process of branching morphogenesis. During the early pseudoglandular stage, the embryonic lung is formed mostly of tubes that keep on branching. As the branching takes place, progenitor cells located in niches are also amplified and progressively differentiate along the proximo-distal and dorso-ventral axes of the lung. Such elaborate processes require coordinated interactions between signaling molecules arising from and acting on four functional domains: the epithelium, the endothelium, the mesenchyme, and the mesothelium. These interactions, quite well characterized in a relatively simple lung tubular structure remain elusive in the successive developmental and postnatal phases of lung development. In particular, a better understanding of the process underlying the formation of secondary septa, key structural units characteristic of the alveologenesis phase, is still missing. This structure is critical for the formation of a mature lung as it allows the subdivision of saccules in the early neonatal lung into alveoli, thereby considerably expanding the respiratory surface. Interruption of alveologenesis in preterm neonates underlies the pathogenesis of chronic neonatal lung disease known as bronchopulmonary dysplasia. De novo formation of secondary septae appears also to be the limiting factor for lung regeneration in human patients with emphysema. In this review, we will therefore focus on what is known in terms of interactions between the different lung compartments and discuss the current understanding of mesenchymal cell lineage formation in the lung, focusing on secondary septae formation.
Clear suppression of Th1 responses but marginal amelioration of autoimmune manifestations by IL-12p40 transgene in MRL-FAS(lprcg)/FAS(lprcg) mice.

To investigate the effects of overproduction of IL-12p40, a potent antagonist against IL-12, on lupus-like autoimmune disease in vivo, we generated p40 transgenic MRL-Fas(lprcg)/Fas(lprcg) mice. Serum p40 and IL-12 levels were 600- to 8000-fold and 3- to 20-fold higher in transgenic (p40-lpr(cg)) than nontransgenic (lpr(cg)) mice, respectively. Serum IFN-gamma levels increased after 3 months of age in lpr(cg) and this age-related increase was completely abrogated in p40-lpr(cg). Serum IL-4 levels were the same in both mice. Production of IgM and IgG anti-double-stranded DNA (dsDNA) antibodies was significantly lower in p40-lpr(cg). Anti-dsDNA antibodies decreased in Th1-dependent IgG2a but increased in the Th2-dependent IgG1 subclass significantly in p40-lpr(cg). Proteinuria, glomerulonephritis, and survival were only marginally ameliorated in p40-lpr(cg). The results suggest that excess p40 production in vivo may suppress Th1 responses in autoantibody and IFN-gamma production but lead to minimal improvement of clinical manifestations of autoimmune disease in this mouse model.
Nonprotein-bound iron in postasphyxial reperfusion injury of the newborn.

OBJECTIVE To investigate if the availability of nonprotein-bound iron after birth asphyxia is related to the severity of the postasphyxial injury and neurodevelopmental outcome. METHODS Nonprotein-bound iron (bleomycin assay) and thiobarbituric-acid-reactive species, an index of oxidative lipid damage, were measured in plasma of 50 newborn infants (gestational age > 34 weeks) between 0 to 8 hours, 8 to 16 hours, and 16 to 24 hours after birth. Three groups were compared: healthy infants (n = 20), moderately asphyxiated infants (n = 15), who were neurologically normal during the first 24 hours after birth and severely asphyxiated infants (n = 15), who developed abnormal neurological signs in the first 24 hours after birth. RESULTS In the severely asphyxiated infants, liver enzymes, creatinine, urea, and uric acid concentrations were significantly elevated. Eleven severely asphyxiated infants were brain-damaged, 9 of them died during the neonatal period. Nonprotein-bound iron was detectable in 30% of the control, 60% of the moderately asphyxiated, and 80% of the severely asphyxiated infants. During the whole study period nonprotein-bound iron concentration was significantly elevated in severely asphyxiated infants as compared with controls. Three of the four severely asphyxiated infants who had a normal outcome at 1 year of age, had no detectable nonprotein-bound iron during the study period. Stepwise logistic regression analysis with neurodevelopmental outcome at 1 year of age (normal versus adverse/death) as dependent variable and all the measured parameters for organ damage as independent variables revealed that the nonprotein-bound iron concentration at 0 to 8 hours after birth was the most significant variable and at the same time the only variable that entered the model, in relation to neurodevelopmental outcome. Thiobarbituric-acid-reactive species tended to be higher in severely asphyxiated infants, suggesting oxidative lipid damage. CONCLUSION Nonprotein-bound iron may play an important role in oxidative damage-mediated postasphyxial brain injury and subsequent neurodevelopmental outcome.
Mast cell density in oral submucous fibrosis: a possible role in pathogenesis.

OBJECTIVE Oral submucous fibrosis (OSMF) is a premalignant condition of oral cavity characterized by inflammation and progressive mucosal fibrosis. It has questionable pathogenesis. Mast cells (MC) have been associated with variety of inflammatory and fibrotic conditions, but little is known about their role in OSMF. Mast cells have been studied in normal gingiva, chronic inflammatory gingivitis, desquamative gingivitis, lichen planus, OSMF and oral cancer. Mast cells exhibit phenotypic plasticity. There is variation in the mast cell mediators with the change in the microenvironment, which makes the study of this cell in various diseases interesting. STUDY DESIGN A retrospective study was conducted to find possible correlation between MC in 25 cases of OSF, 10 cases of oral squamous cell carcinoma (OSCC) and 10 cases of normal buccal mucosa by means of acidified toluidine blue staining method. RESULTS The density of MC increased with disease progression. The densities of MC were significantly higher in OSMF than in normal buccal mucosa (p=0.001). The average numbers of MCs per square millimeter were 25, 49.50, 53.25 & 55.25 respectively. CONCLUSION The results suggest that MC have a definite role in initiation and progression of OSMF.
Preferred therapies for neovascular age-related macular degeneration

Purpose of review This report reviews the current treatment strategies and the most recent clinical trials in the treatment of neovascular age-related macular degeneration. Recent findings The functional and anatomic outcomes achieved in the pivotal ranibizumab trials with monthly injections set the standard for comparison. Since then, various modified dosing regimens with the aim of lessening the treatment burden associated with monthly injections have been investigated. Additionally, level I evidence now exists for the noninferiority of bevacizumab, as compared to ranibizumab, in the treatment of neovascular age-related macular degeneration (AMD) through 1 year of follow-up. Aflibercept has emerged as a new anti- vascular endothelial growth factor (VEGF) therapy showing encouraging treatment results at 1 year. Novel treatments combined with anti-VEGF agents such as localized radiation are currently being investigated. Summary Anti-VEGF monotherapy remains the preferred therapy for the management of neovascular AMD at the present time. Aflibercept is a new, FDA-approved, effective, anti-VEGF agent available for clinical use. Ongoing clinical trials will help determine the optimal dosing regimens for all of these agents, as well as the long-term efficacy and safety of combination therapy modalities.
Toxicity of Nanoparticles and an Overview of Current Experimental Models

Nanotechnology is a rapidly growing field having potential applications in many areas. Nanoparticles (NPs) have been studied for cell toxicity, immunotoxicity, and genotoxicity. Tetrazolium-based assays such as MTT, MTS, and WST-1 are used to determine cell viability. Cell inflammatory response induced by NPs is checked by measuring inflammatory biomarkers, such as IL-8, IL-6, and tumor necrosis factor, using ELISA. Lactate dehydrogenase (LDH) assay is used for cell membrane integrity. Different types of cell cultures, including cancer cell lines have been employed as in vitro toxicity models. It has been generally agreed that NPs interfere with either assay materials or with detection systems. So far, toxicity data generated by employing such models are conflicting and inconsistent. Therefore, on the basis of available experimental models, it may be difficult to judge and list some of the more valuable NPs as more toxic to biological systems and vice versa. Considering the potential applications of NPs in many fields and the growing apprehensions of FDA about the toxic potential of nanoproducts, it is the need of the hour to look for new internationally agreed free of bias toxicological models by focusing more on in vivo studies.
Governing bodies: a Foucaultian critique of Paralympic power relations

In this thesis, I use Foucault’s methods of discourse analysis and genealogy, and my own experiences as a Paralympic athlete, to analyze and critique the power relations of the Paralympic Movement. In Chapter 1, I contextualize my study by discussing relevant literature in Critical Disability Studies, Sociology of Sport and Adapted Physical Activity, and by introducing my methodological and epistemological frameworks. In Chapter 2, I analyze two historical accounts of the Paralympic Movement to demonstrate how they discursively represent, reproduce and justify Paralympic power relations. In Chapters 3 through 5, I use genealogy to critique Paralympic power relations: analyzing their systems of differentiation, types of objectives, instrumental modes, forms of institutionalization and degrees of rationalization. This analysis brings to the forefront how discourses of empowerment reproduce, justify and conceal the increasingly rationalized structures that enable Paralympic experts to act upon the actions, bodies and identities of those experiencing disabilities.
Microsurgical anatomy of cerebral revascularization. Part II: posterior circulation.

OBJECT Revascularization is an important component of treatment for complex aneurysms, skull base tumors, and vertebrobasilar ischemia in the posterior circulation. In this study, the authors examined the microsurgical anatomy related to cerebral revascularization in the posterior circulation and demonstrate various procedures for bypass surgery. METHODS The microsurgical anatomy of cerebral and cerebellar vessels as they relate to revascularization procedure and techniques, including extracranial-to-intracranial bypass grafting, arterial interposition grafting, and side-to-side anastomosis, were examined by performing stepwise dissections in 22 adult cadaveric specimens. The arteries and veins in the specimens were perfused with colored silicone. Dominant cerebral and cerebellar revascularization procedures in the posterior circulations include superficial temporal artery (STA)-posterior cerebral artery (PCA), STA-superior cerebellar artery (SCA), occipital artery (OA)-anterior inferior cerebellar artery, OA-posterior inferior cerebellar artery (PICA), and PICA-PICA anastomoses. These procedures are effective in relatively small but critical areas including the brainstem and cerebellum. For revascularization of larger areas a saphenous vein graft is used to create a bypass between the PCA and the external carotid artery. Surgical procedures are generally difficult to perform in deep and narrow operative spaces near critical vital structures. CONCLUSIONS Although a clear guideline for cerebral revascularization procedures has not yet been established, it is important to understand various microsurgical techniques and their related anatomical structures. This will help surgeons consider surgical indications for treatment of patients with vertebrobasilar ischemia caused by aneurysms, tumors, or atherosclerotic diseases in the posterior circulation.
Validation and description of two new north-western Australian Rainbow skinks with multispecies coalescent methods and morphology

While methods for genetic species delimitation have noticeably improved in the last decade, this remains a work in progress. Ideally, model based approaches should be applied and considered jointly with other lines of evidence, primarily morphology and geography, in an integrative taxonomy framework. Deep phylogeographic divergences have been reported for several species of Carlia skinks, but only for some eastern taxa have species boundaries been formally tested. The present study does this and revises the taxonomy for two species from northern Australia, Carlia johnstonei and C. triacantha. We introduce an approach that is based on the recently published method StarBEAST2, which uses multilocus data to explore the support for alternative species delimitation hypotheses using Bayes Factors (BFD). We apply this method, jointly with two other multispecies coalescent methods, using an extensive (from 2,163 exons) data set along with measures of 11 morphological characters. We use this integrated approach to evaluate two new candidate species previously revealed in phylogeographic analyses of rainbow skinks (genus Carlia) in Western Australia. The results based on BFD StarBEAST2, BFD* SNAPP and BPP genetic delimitation, together with morphology, support each of the four recently identified Carlia lineages as separate species. The BFD StarBEAST2 approach yielded results highly congruent with those from BFD* SNAPP and BPP. This supports use of the robust multilocus multispecies coalescent StarBEAST2 method for species delimitation, which does not require a priori resolved species or gene trees. Compared to the situation in C. triacantha, morphological divergence was greater between the two lineages within Kimberley endemic C. johnstonei, which also had deeper divergent histories. This congruence supports recognition of two species within C. johnstonei. Nevertheless, the combined evidence also supports recognition of two taxa within the more widespread C. triacantha. With this work, we describe two new species, Carlia insularis sp. nov and Carlia isostriacantha sp. nov. in the northwest of Australia. This contributes to increasing recognition that this region of tropical Australia has a rich and unique fauna.
Cognitive and Social Factors Affecting Arab Students Learning English as a Third Language in Israel

This study investigated the relationship between attitudes, interest, and culture, on the one hand, and reading comprehension in English as a third language on the other. Participants were 70 native Arab students aged 15-16 who were sampled from three high schools in the Haifa area. They were administered an attitude questionnaire, culturally familiar and culturally unfamiliar stories, multiple-choice comprehension questions and an interest questionnaire. The results revealed that the Arab students' attitudes towards English were more instrumental than integrative; and their attitude towards the English learning situation was positive. Further, they were more interested in the familiar cultural story than in the unfamiliar one, and they performed accordingly. In other words, type of text (culturally familiar/unfamiliar) was the only significant predictor of reading comprehension in English. However, when reading scores were regressed on the independent variables controlling for text type, interest was the only significant predictor. Thus, in the Israeli-Arab situation, learner interest and type of text are powerful predictors of comprehension of English as a third language.
The Effectiveness of Online Shopping Characteristics and Well-Designed Websites on Satisfaction

Electronic commerce has grown rapidly in recent years. However, surveys of online customers continue to indicate that many remain unsatisfied with their online purchase experiences. Clearly, more research is needed to better understand what affects customers' evaluations of their online experiences. Through a large dataset gathered from two online websites, this study investigates the importance of product uncertainty and retailer visibility in customers' online purchase decisions, as well as the mitigating effects of retailer characteristics. We find that high product uncertainty and low retailer visibility have a negative impact on customer satisfaction. However, a retailer's service quality, website design, and pricing play important roles in mitigating the negative impact of high product uncertainty and low retailer visibility. Specifically, service quality can mitigate the negative impacts of low retailer visibility and high product uncertainty in online markets. Website design, on the other hand, helps to reduce the impact of product uncertainty when experience goods are involved.
Attitudes Toward English and Its Functions in Finland: A Discourse-Analytic Study.

With the second cognitive revolution, a new paradigm is emerging in (social) psychology: positivism is giving way to social constructionism. Consequently, this paper argues for a redefinition of terms and reconsideration of methodology in research on language attitudes. More specifically, it argues that mentalistic definitions of attitudes be replaced with social ones, and experimentation with the matched-guise technique with discourse-analytic research. These developments are illustrated with a qualitative study concerning the attitudes or views held by college students towards English in Finland, based on their written responses to a letter-to-the-Editor that basically argued against the use of English in the country for a number of reasons.
Student and Environmental Protests in Chile: The Role of Social Media

In 2011, Chile experienced two massive protest movements – one against the cost and quality of public education and another against the construction of power plants in Patagonia. This represented a unique opportunity to analyse and compare how Facebook and Twitter use were related to street demonstrations. Using a probabilistic face-to-face survey among urban youth (18–29 years old) conducted shortly after the protests, this study revealed a positive relationship between the use of social media and participation in both social movements, even controlling for other relevant variables (e.g. political interest, ideology and trust). The theoretical and methodological implications of these findings are discussed in this article.