Miracle-dz/UniFAR-FTUs huggingface dataset:
In-vivo administration of CLC-K kidney chloride channels inhibitors increases water diuresis in rats: a new drug target for hypertension?

Objective The human kidney-specific chloride channels ClC-Ka (rodent ClC-K1) and ClC-Kb (rodent ClC-K2) are important determinants of renal function, participating to urine concentration and blood pressure regulation mechanisms. Here we tested the hypothesis that these chloride channels could represent new drug targets for inducing diuretic and antihypertensive effects. Methods To this purpose, the CLC-K blockers benzofuran derivatives MT-189 and RT-93 (10, 50, 100 mg/kg), were acutely administered by gavage in Wistar rats, and pharmacodynamic and pharmacokinetic parameters determined by functional, bioanalytical, biochemical and molecular biology assays. Results Plasma concentration values for MT-189 and RT-93 were indicative of good bioavailability. Both MT-189 and RT-93 dose-dependently increased urine volume without affecting electrolyte balance. A comparable reduction of SBP was observed in rats after MT-189, RT-93 or furosemide administration. Benzofuran derivatives treatment did not affect kidney CLC-K mRNA level or inner medulla osmolality, whereas a significant vasopressin-independent down-regulation of aquaporin water channel type 2 was observed at protein and transcriptional levels. In rats treated with benzofuran derivatives, the observed polyuria was mainly water diuresis; this finding indirectly supports a cross-talk between chloride and water transport in nephron. Moreover, preliminary in-vitro evaluation of the drugs capability to cross the blood-inner ear barrier suggests that these compounds have a limited ability to induce potential auditory side effects. Conclusion CLC-K blockers may represent a new class of drugs for the treatment of conditions associated with expanded extracellular volume, with a hopeful high therapeutic potential for hypertensive patients carrying ClC-K gain-of-function polymorphisms.
Freeze after writing: quasi-deterministic parallel programming with LVars

Deterministic-by-construction parallel programming models offer the advantages of parallel speedup while avoiding the nondeterministic, hard-to-reproduce bugs that plague fully concurrent code. A principled approach to deterministic-by-construction parallel programming with shared state is offered by LVars: shared memory locations whose semantics are defined in terms of an application-specific lattice. Writes to an LVar take the least upper bound of the old and new values with respect to the lattice, while reads from an LVar can observe only that its contents have crossed a specified threshold in the lattice. Although it guarantees determinism, this interface is quite limited. We extend LVars in two ways. First, we add the ability to "freeze" and then read the contents of an LVar directly. Second, we add the ability to attach event handlers to an LVar, triggering a callback when the LVar's value changes. Together, handlers and freezing enable an expressive and useful style of parallel programming. We prove that in a language where communication takes place through these extended LVars, programs are at worst quasi-deterministic: on every run, they either produce the same answer or raise an error. We demonstrate the viability of our approach by implementing a library for Haskell supporting a variety of LVar-based data structures, together with a case study that illustrates the programming model and yields promising parallel speedup.
Context-Dependent Plastic Response during Egg-Laying in a Widespread Newt Species

Previous research on predator-induced phenotypic plasticity mostly focused on responses in morphology, developmental time and/or behaviour during early life stages, but the potential significance of anticipatory parental responses has been investigated less often. In this study I examined behavioural and maternal responses of gravid female smooth newts, Lissotriton vulgaris, in the presence of chemical cues originating from invertebrate predators, Acilius sulcatus water beetles and Aeshna cyanea dragonfly larvae. More specifically, I tested the extent of oviposition preference, plasticity in egg-wrapping behaviour and plasticity in egg size when females had the possibility to lay eggs at oviposition sites with and without predator cues during overnight trials. I found that individuals did not avoid laying eggs in the environment with predator cues; however, individuals that deposited eggs into both environments adjusted the size of the laid eggs to the perceived environment. Females deposited larger eggs earlier in the season but egg size decreased with time in the absence of predator cues, whereas individuals laid eggs of average size throughout the investigated reproductive period when such cues were present. Also, egg size was found to be positively related to hatching success. Individuals did not adjust their wrapping behaviour to the presence of predator cues, but females differed in the extent of egg-wrapping between ponds. Females’ body mass and tail depth were also different between ponds, whereas their body size was positively associated with egg size. According to these results, female smooth newts have the potential to exhibit activational plasticity and invest differently into eggs depending on temporal and environmental factors. Such an anticipatory response may contribute to the success of this caudate species under a wide range of predator regimes at its natural breeding habitats.
Population Dynamics of a Salmonella Lytic Phage and Its Host: Implications of the Host Bacterial Growth Rate in Modelling

The prevalence and impact of bacteriophages in the ecology of bacterial communities coupled with their ability to control pathogens turn essential to understand and predict the dynamics between phage and bacteria populations. To achieve this knowledge it is essential to develop mathematical models able to explain and simulate the population dynamics of phage and bacteria. We have developed an unstructured mathematical model using delay-differential equations to predict the interactions between a broad-host-range Salmonella phage and its pathogenic host. The model takes into consideration the main biological parameters that rule phage-bacteria interactions likewise the adsorption rate, latent period, burst size, bacterial growth rate, and substrate uptake rate, among others. The experimental validation of the model was performed with data from phage-interaction studies in a 5 L bioreactor. The key and innovative aspect of the model was the introduction of variations in the latent period and adsorption rate values that are considered as constants in previous developed models. By modelling the latent period as a normal distribution of values and the adsorption rate as a function of the bacterial growth rate it was possible to accurately predict the behaviour of the phage-bacteria population. The model was shown to predict simulated data with a good agreement with the experimental observations and explains how a lytic phage and its host bacteria are able to coexist.
Impact of Laser Mode Partition Noise on Optical Heterodyning at Millimeter-Wave Frequencies

This paper examines the impact of laser mode partition noise (LMPN) during optical heterodyning process. The system presented here employs a passively mode-locked laser diode (PMLLD) as a multimode source for millimeter-wave (mm-wave) generation. A comprehensive theoretical and experimental study of LMPN at low frequency and, for the first time, in mm-wave frequency band is demonstrated. Simulations are presented to investigate the distribution of mode partition noise across optical modes. LMPN appears as an impairment in performance on a radio-over-fiber (RoF) communication system by increasing the intensity noise when the number of optical modes detected by a photodiode (PD) is limited. The effect of LMPN for various number of modes is demonstrated by coherent and incoherent receivers, and it is shown that 25 dB degradation in signal power occurs due to noise when not all modes are detected.
Forcing of the Arctic Oscillation by Eurasian Snow Cover

AbstractThroughout much of the latter half of the twentieth century, the dominant mode of Northern Hemisphere (NH) extratropical wintertime circulation variability—the Arctic Oscillation (AO)—exhibited a positive trend, with decreasing high-latitude sea level pressure (SLP) and increasing midlatitude SLP. General circulation models (GCMs) show that this trend is related to several factors, including North Atlantic SSTs, greenhouse gas/ozone-induced stratospheric cooling, and warming of the Indo-Pacific warm pool. Over the last approximately two decades, however, the AO has been decreasing, with 2009/10 featuring the most negative AO since 1900. Observational and idealized modeling studies suggest that snow cover, particularly over Eurasia, may be important. An observed snow–AO mechanism also exists, involving the vertical propagation of a Rossby wave train into the stratosphere, which induces a negative AO response that couples to the troposphere. Similar to other GCMs, the authors show that transient sim.
An Integrated Framework for High Angular Resolution Diffusion Imaging-Based Investigation of Structural Connectivity

Structural connectivity models hold great promise for expanding what is known about the ways information travels throughout the brain. The physiologic interpretability of structural connectivity models depends heavily on how the connections between regions are quantified. This article presents an integrated structural connectivity framework designed around such an interpretation. The framework provides three measures to characterize the structural connectivity of a subject: (1) the structural connectivity matrix describing the proportion of connections between pairs of nodes, (2) the nodal connection distribution (nCD) characterizing the proportion of connections that terminate in each node, and (3) the connection density image, which presents the density of connections as they traverse through white matter (WM). Individually, each possesses different information concerning the structural connectivity of the individual and could potentially be useful for a variety of tasks, ranging from characterizing and localizing group differences to identifying novel parcellations of the cortex. The efficiency of the proposed framework allows the determination of large structural connectivity networks, consisting of many small nodal regions, providing a more detailed description of a subject's connectivity. The nCD provides a gray matter contrast that can potentially aid in investigating local cytoarchitecture and connectivity. Similarly, the connection density images offer insight into the WM pathways, potentially identifying focal differences that affect a number of pathways. The reliability of these measures was established through a test/retest paradigm performed on nine subjects, while the utility of the method was evaluated through its applications to 20 diffusion datasets acquired from typically developing adolescents.
A note on estimation using quantized data

Abstract In this paper we discuss estimation of parameters from quantized data. Extending some recent results appeared in the literature we show that, under a regularity assumption on the parametric model describing the data, the Maximum Likelihood estimator can be found, asymptotically, in closed form in two steps. The first is a non-linear (and invertible) mapping of the observed relative frequencies which makes the dependence on the parameters linear; the second is a linear estimator. Some simulations which demonstrate the results are included.
Bovine Viral Diarrhea Virus (BVDV) in White-Tailed Deer (Odocoileus virginianus)

Bovine viral diarrhea virus (BVDV) is the prototypic member of the genus Pestivirus in the family Flaviviridae. Infections with BVDV cause substantial economic losses to the cattle industries, prompting various organized control programs in several countries. In North America, these control programs are focused on the identification and removal of persistently infected (PI) cattle, enhancement of BVDV-specific immunity through vaccination, and the implementation of biosecure farming practices. To be successful, control measures must be based on complete knowledge of the epidemiology of BVDV, including the recognition of other potential sources of the virus. BVDV does not possess strict host-specificity, and infections of over 50 species in the mammalian order Artiodactyla have been reported. Over 50 years ago, serologic surveys first suggested the susceptibility of white-tailed deer (Odocoileus virginianus), the most abundant free-ranging ruminant in North America, to BVDV. However, susceptibility of white-tailed deer to BVDV infection does not alone imply a role in the epidemiology of the virus. To be a potential wildlife reservoir, white-tailed deer must: (1) be susceptible to BVDV, (2) shed BVDV, (3) maintain BVDV in the population, and (4) have sufficient contact with cattle that allow spillback infections. Based on the current literature, this review discusses the potential of white-tailed deer to be a reservoir for BVDV.
Cognitive Training for Schizophrenia in Developing Countries: A Pilot Trial in Brazil

Cognitive deficits in schizophrenia can massively impact functionality and quality of life, furthering the importance of cognitive training. Despite the development of the field in Europe and in the United States, no programmes have been developed and tested in developing countries. Different cultural backgrounds, budget restrictions, and other difficulties may render treatment packages created in high income countries difficult for adoption by developing nations. We performed a pilot double-blind, randomized, controlled trial in order to investigate the efficacy and feasibility of an attention and memory training programme specially created in a developing nation. The intervention used simple, widely available materials, required minimal infrastructure, and was conducted in groups. The sample included seventeen stable Brazilians with schizophrenia. Sessions were conducted weekly during five months. The cognitive training group showed significant improvements in inhibitory control and set-shifting over time. Both groups showed improvements in symptoms, processing speed, selective attention, executive function, and long-term visual memory. Improvements were found in the control group in long-term verbal memory and concentration. Our findings reinforce the idea that cognitive training in schizophrenia can be constructed using simple resources and infrastructure, facilitating its adoption by developing countries, and it may improve cognition.
PTSD and DNA Methylation in Select Immune Function Gene Promoter Regions: A Repeated Measures Case-Control Study of U.S. Military Service Members

Background: The underlying molecular mechanisms of PTSD are largely unknown. Distinct expression signatures for PTSD have been found, in particular for immune activation transcripts. DNA methylation may be significant in the pathophysiology of PTSD, since the process is intrinsically linked to gene expression. We evaluated temporal changes in DNA methylation in select promoter regions of immune system-related genes in U.S. military service members with a PTSD diagnosis, pre- and post-diagnosis, and in controls. Methods: Cases (n = 75) had a post-deployment diagnosis of PTSD in their medical record. Controls (n = 75) were randomly selected service members with no PTSD diagnosis. DNA was extracted from pre- and post-deployment sera. DNA methylation (%5-mC) was quantified at specific CpG sites in promoter regions of insulin-like growth factor 2 (IGF2), long non-coding RNA transcript H19, interleukin-8 (IL8), IL16, and IL18 via pyrosequencing. We used multivariate analysis of variance and generalized linear models to calculate adjusted means (adjusted for age, gender, and race) to make temporal comparisons of %5-mC for cases (pre- to post-deployment) versus controls (pre- to post-deployment). Results: There were significant differences in the change of %5-mC pre- to post-deployment between cases and controls for H19 (cases: +0.57%, controls: −1.97%; p = 0.04) and IL18 (cases: +1.39%, controls: −3.83%; p = 0.01). For H19 the difference was driven by a significant reduction in %5-mC among controls; for IL18 the difference was driven by both a reduction in %5-mC among controls and an increase in %5-mC among cases. Stratified analyses revealed more pronounced differences in the adjusted means of pre-post H19 and IL18 methylation differences for cases versus controls among older service members, males, service members of white race, and those with shorter deployments (6–12 months). Conclusion: In the study of deployed personnel, those who did not develop PTSD had reduced %5-mC levels of H19 and IL18 after deployment, while those who did develop PTSD had increased levels of IL18. Additionally, pre-deployment the people who later became cases had lower levels of IL18 %5-mC compared with controls. These findings are preliminary and should be investigated in larger studies.
Human bronchoalveolar lavage fluid: Two‐dimensional gel electrophoresis, amino acid microsequencing and identification of major proteins

Although bronchoalveolar lavage has been used as a research and clinical tools for more than two decades to investigate the cellular and soluble components of the lower respiratory tract, its exact protein composition has never been established. In this context, proteins of human bronchoalveolar lavage fluids (BALF), obtained by washing the epithelial lining fluid of the lungs with phosphate‐buffered saline, were analyzed by two‐dimensional electrophoresis (2‐DE) under denaturing and reducing conditions. To characterize the widest amount of proteins, an analytical map of human bronchoalveolar lavage fluid proteins has been created from a pool of BALF from various patients. The resulting map comprises 211 silver‐stained spots in the range of pI 3.5—10 and molecular mass 5—100 kDa. We identified 182 spots by microsequence analysis and by matching with human blood plasma and the Macrophage Like Cell line reference 2‐DE maps available from the SWISS‐2DPAGE database. The human bronchoalveolar lavage fluid was found to contain 61 different proteins or isoforms thereof. Most of the proteins had low molecular masses (< 35 kDa) and rather acidic isoelectric points (pI; 4 < pI < 7). The proteins in the lavage either are produced locally or originate from plasma. Two unknown proteins were identified and are currently under investigation.
Setting up Cows for First Postpartum Artificial Insemination

A long standing goal of reproductive physiologists was to develop a hormonal synchronization program that could overcome the problems and limitations associated with visual detection of estrus in dairy cattle. This goal was realized in 1995 with the publication of a hormonal synchronization protocol that combined GnRH and PGF2α to control ovarian physiology and is now commonly referred to as the Ovsynch protocol (Pursley et al., 1995). The Ovsynch protocol synchronizes follicular development, luteal regression and ovulation such that artificial insemination can be conducted at a fixed-time without the need for estrus detection, commonly referred to as timed artificial insemination (TAI). Subsequent studies that repeated this work soon verified the results of the original publication (Burke et al., 1996; Pursley et al., 1997a,b), and dairy producers and veterinarians began to implement the Ovsynch protocol as a tool for reproductive management on commercial dairies. In the nearly 15 years since this first publication, today many dairy farms in the U.S. and around the world have adopted systematic synchronization protocols as a routine strategy for submitting cows for first and greater postpartum AI service (Caraviello et al., 2006).
Doubly Bound : The Impact of Gender and Race on the Politics of Black Women

Gender has been thought to be less salient than race among black women. Data from two national surveys of black Americans, conducted in 1984 and 1996, show that black women identify as strongly on the basis of their gender as their race, and that these gender and racial identities are mutually reinforcing. Nevertheless, among black women, their identification with their race more powerfully affected their political attitudes than did their identification on the basis of gender, except in instances where the interests of blacks directly conflict with the interests of women. These empirically based findings speak to the issue of why the attitudes of black women toward contemporary gender issues can sharply diverge from those of white women.
A SUMMARY OF EVIDENCE ON RADIATION EXPOSURES RECEIVED NEAR TO THE SEMIPALATINSK NUCLEAR WEAPONS TEST SITE IN KAZAKHSTAN

Abstract— The presently available evidence about the magnitude of doses received by members of the public living in villages in the vicinity of Semipalatinsk nuclear test in Kazakhstan, particularly with respect to external radiation, while preliminary, is conflicting. The village of Dolon, in particular, has been identified for many years as the most highly exposed location in the vicinity of the test site. Previous publications cited external doses of more than 2 Gy to residents of Dolon while an expert group assembled by the WHO in 1997 estimated that external doses were likely to have been less than 0.5 Gy. In 2001, a larger expert group workshop was held in Helsinki jointly by the WHO, the National Cancer Institute of the United States, and the Radiation and Nuclear Safety Authority of Finland, with the expressed purpose to acquire data to evaluate the state of knowledge concerning doses received in Kazakhstan. This paper summarizes evidence presented at that workshop. External dose estimates from calculations based on sparse physical measurements and biodosimetric estimates based on chromosome abnormalities and electron paramagnetic resonance from a relatively small sample of teeth do not agree well. The physical dose estimates are generally higher than the biodosimetric estimates (1 Gy or more compared to 0.5 Gy or less). When viewed in its entirety, the present body of evidence does not appear to support external doses greater than 0.5 Gy; however, research is continuing to try and resolve the difference in dose estimates from the different methods. Thyroid doses from internal irradiation, which can only be estimated via calculation, are expected to have been several times greater than the doses from external irradiation, especially where received by small children.
Massive Multiuser MIMO in Heterogeneous Cellular Networks With Full Duplex Small Cells

Full duplex (FD) communication has emerged as an attractive solution for increasing the network throughput, by allowing downlink (DL) and uplink (UL) transmissions in the same spectrum. However, only employing FD base stations in heterogeneous cellular networks (HCNs) cause coverage reduction, due to the DL and UL interferences as well as the residual loop interference. We, therefore, propose HCNs with half duplex massive multiuser multiple-input multiple-output macrocell base stations (MBSs) to relax the coverage reduction, and FD small cell base stations (SBSs) to improve spectrum efficiency. A tractable framework of the proposed system is presented, which allows to derive exact and asymptotic expressions for the DL and the UL rate coverage probabilities, and the DL and the UL area spectral efficiencies (ASEs). Monte Carlo simulations confirm the accuracy of the analytical results, and it is revealed that the equipping massive number of antennas at MBSs enhances the DL rate coverage probability, whereas increasing FD SBSs increases the DL and the UL ASEs. The results also demonstrate that by tuning the UL fractional power control, a desirable performance in both UL and DL can be achieved.