Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessment of the underestimation of childhood diarrhoeal disease burden in Israel

SUMMARY We determined the extent by which mandatory reporting on isolates of Shigella and Salmonella underestimates the burden of diarrhoeal diseases in individuals aged <17 years in Israel and examined paediatricians' knowledge, attitudes and practices related to patient visits with diarrhoeal diseases. Sources of data were a nationwide population-based telephone survey for presence of diarrhoeal diseases, Maccabi Healthcare Services databases and a mail survey among its paediatricians. Monte Carlo simulation and rate estimates for all stages, from visit to physician to reporting on a culture-confirmed case of shigellosis or salmonellosis, were used to determine the underestimation factor. Of 1492 children, 5·7% reported a diarrhoeal episode during the 2 weeks prior to interview. The rate of visiting a physician with and without fever was 86% and 16%, respectively. A stool culture was performed for around 20% of patients and the isolation rates were 7·1% for Shigella and 2·1% for Salmonella. Paediatricians (n=214) ranked very young age of patient and the complaint ‘bloody diarrhoea’ as the most important determinants. We estimated that one reported isolate of Shigella or Salmonella represented 152 diarrhoeal episodes of all aetiologies. This estimate is important for further assessments of the true burden of diarrhoeal diseases.
Comparative Genomic Analysis Using Microarray Demonstrates a Strong Correlation between the Presence of the 80-Kilobase Pathogenicity Island and Pathogenicity in Kanagawa Phenomenon-Positive Vibrio parahaemolyticus Strains

ABSTRACT Vibrio parahaemolyticus is a gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. Almost all of the clinical V. parahaemolyticus isolates exhibit beta-type hemolysis on Wagatsuma agar, known as the Kanagawa phenomenon (KP). KP is induced by the thermostable direct hemolysin produced by the organism and has been considered a crucial marker to distinguish pathogenic strains from nonpathogenic ones. Since 1996, so-called “pandemic clones,” the majority of which belong to serotype O3:K6, have caused worldwide outbreaks of gastroenteritis. In this study, we used a DNA microarray constructed based on the genome sequence of a pandemic V. parahaemolyticus strain, RIMD2210633, to examine the genomic composition of 22 strains of V. parahaemolyticus, including both pathogenic (pandemic and nonpandemic) and nonpathogenic strains. More than 86% of the RIMD2210633 genes were conserved in all of the strains tested. Many variably present genes formed gene clusters on the genome of RIMD2210633 and were probably acquired through lateral gene transfer. At least 65 genes over 11 loci were specifically present in the pandemic strains compared with any of the nonpandemic strains, suggesting that the difference between pandemic and nonpandemic strains is not due to a simple genetic event. Only the genes in the 80-kb pathogenicity island (Vp-PAI) on chromosome II, including two tdh genes and a set of genes for the type III secretion system, were detected only in the KP-positive pathogenic strains. These results strongly suggest that acquisition of this Vp-PAI was crucial for the emergence of V. parahaemolyticus strains that are pathogenic for humans.
The Role of Family-led Disability Organizations in Supporting Families with Hearing-Related Concerns

2Hands & Voices Abstract A survey was conducted with state level chapters from Family Voices, Parent Training and Information Centers, and Parent–to-Parent USA to understand how their current activities support families of children with hearing-related concerns and to identify gaps in their ability to support families of children who are deaf or hard of hearing (DHH). These organizations reported that they are contacted with parent requests for information in regard to family support opportunities, early intervention, referral sources pertaining to hearing concerns, financial help, and providing information about legal rights. Results showed that the greatest challenges for these organizations were related to needing to connect families to financial resources pertaining to hearing-related needs, engaging families of children who are DHH in their organization’s activities, having resources available in other languages, and identifying pediatric providers that serve DHH children. Potential ways to strengthen the capacity of these organizations to meet the needs of families with hearing-related concerns as well as increasing their awareness of partnerships with the EHDI system are discussed.
The Global Burden of Chronic Kidney Disease: How Valid Are the Estimates?

Background/Aims: The values for the global prevalence of chronic kidney disease (CKD) are poorly understood. Current classification schemas may overstate the prevalance of CKD. This minireview analyzes the pitfalls in the use of current classification approaches for identifying CKD on a global basis. Methods: Literature review and comment. Results: Published estimates for the global burden of CKD are likely to be incorrect and inflated. Overestimations of prevalence have occurred due to flaws in the classification systems employed and in ascertainment methods. Conclusions: A revision of the current system of diagnosing and classifying CKD is needed in order to determine with greater precision true global burden of CKD. A new system is proposed.
Characterization of Native and Recombinant Falcipain-2, a Principal Trophozoite Cysteine Protease and Essential Hemoglobinase ofPlasmodium falciparum *

Trophozoites of the malaria parasitePlasmodium falciparum hydrolyze erythrocyte hemoglobin in an acidic food vacuole to provide amino acids for parasite protein synthesis. Cysteine protease inhibitors block hemoglobin degradation, indicating that a cysteine protease plays a key role in this process. A principal trophozoite cysteine protease was purified by affinity chromatography. Sequence analysis indicated that the protease is encoded by a previously unidentified gene, falcipain-2. Falcipain-2 was predominantly expressed in trophozoites, was concentrated in food vacuoles, and was responsible for at least 93% of trophozoite soluble cysteine protease activity. A construct encoding mature falcipain-2 and a small portion of the prodomain was expressed in Escherichia coli and refolded to active enzyme. Specificity for the hydrolysis of peptide substrates by native and recombinant falcipain-2 was very similar, and optimal at acid pH in a reducing environment. Under physiological conditions (pH 5.5, 1 mm glutathione), falcipain-2 hydrolyzed both native hemoglobin and denatured globin. Our results suggest that falcipain-2 can initiate cleavage of native hemoglobin in the P. falciparum food vacuole, that, following initial cleavages, the protease plays a key role in rapidly hydrolyzing globin fragments, and that a drug discovery effort targeted at this protease is appropriate.
Coexpression of UL20p and gK Inhibits Cell-Cell Fusion Mediated by Herpes Simplex Virus Glycoproteins gD, gH-gL, and Wild-Type gB or an Endocytosis-Defective gB Mutant and Downmodulates Their Cell Surface Expression

ABSTRACT Syncytium formation in cells that express herpes simplex virus glycoprotein B (gB), gD, gH, and gL is blocked by gK (E. Avitabile, G. Lombardi, and G. Campadelli-Fiume, J. Virol. 77:6836-6844, 2003). Here, we report the results of two series of experiments. First, UL20 protein (UL20p) expression weakly inhibited cell-cell fusion. Coexpression of UL20p and gK drastically reduced fusion in a cell-line-dependent manner, with the highest inhibition in BHK cells. Singly expressed UL20p and gK localized at the endoplasmic reticulum and nuclear membranes. When they were coexpressed, both proteins relocalized to the Golgi apparatus. Remarkably, in cells that coexpressed UL20p and gK, the antifusion activity correlated with a downmodulation of gD, gB, gH, and gL cell surface expression. Second, gBΔ867 has a partial deletion in the cytoplasmic tail that removed endocytosis motifs. Whereas wild-type (wt) gB was internalized in vesicles lined with the endosomal marker Rab5, gBΔ867 was not internalized, exhibited enhanced cell surface expression, and was more efficient in mediating cell-cell fusion than wt gB. The antifusion activity of UL20p and gK was also exerted when gBΔ867 replaced wt gB in the cell fusion assay. These studies show that the gB C tail carries a functional endocytosis motif(s) and that the removal of the motif correlated with increased gB surface expression and increased fusion activity. We conclude that cell-cell fusion in wt-virus-infected cells is negatively controlled by at least two mechanisms. The novel mechanism described here involves the concerted action of UL20p and gK and correlates with a moderate but consistent reduction in the cell surface expression of the fusion glycoproteins. This mechanism is independent of the one exerted through endocytosis-mediated downmodulation of gB from the plasma membrane.
Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface

Ag-implanted titanium with a nanostructured surface was prepared by hydrothermal treatment with H2O2 followed by Ag plasma immersion ion implantation. Streptococcus mutans, Porphyromonas gingivalis and Candida albicans were chosen for antimicrobial tests. Genes related to microbial structure or adhesion, namely glucan-binding proteins B (GbpB), fimbria protein A (FimA), and agglutinin-like sequence4 (Als4), were examined. The osteoblast’s attachment, viability, and quantitative analysis of osteogenic gene expression (Alp, Ocn, RunX2) on titanium surfaces were evaluated. Scanning electron microscopy (SEM) revealed that Ag nanoparticles of approximately 10 nm were incorporated on the nanostructured surface of titanium after Ag plasma immersion ion implantation. Trials showed that 93.99% of S. mutans, 93.57% of P. g, and 89.78% of C. albicans were killed on the Ag-implanted titanium with a nanostructured surface. Gene expressions from the three microorganisms confirmed the antimicrobial activities of the Ag-implanted titanium with a nanostructured surface. Furthermore, the adhesive images and viability assays indicated that the Ag-implanted titanium with a nanostructured surface did not impair osteoblasts. The expressions of osteoblast phenotype genes in cells grown on the Ag-implanted titanium surface were significantly increased. The results of this study suggest that the Ag-implanted titanium with a nanostructured surface displays good antimicrobial properties, reducing gene expressions of microorganisms, and excellent cell adhesion and osteogenic effects.
Differences in emotional intelligence and team cohesiveness in men's and women's community college athletic teams

This study contributes to the sports psychology literature by examining an existing paucity in the application of Emotional Intelligence (EI) theory and models to the athletic domain. Four branches of the ability model of EI (perceiving emotions, using emotions, understanding emotions, and managing emotions) were examined in relation to team cohesiveness (task, social, and overall) and team performance disaggregated by gender. The Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT) was used to assess the EI level of participants. Team cohesiveness was measured using the Group Environment Questionnaire (GEQ). The findings indicted that EI only had a significant effect on team cohesiveness social when moderated by gender, while the other relationships were not found to be statistically significant when moderated by gender. No significant relationship was discovered between EI and team cohesiveness in female athletes. No significant difference existed between males and females on a model containing four branches of emotional intelligence. A significant difference between gender and team cohesiveness was found. The final analysis of emotional intelligence, team cohesiveness, and team performance indicated that a significant relationship did exist. Team cohesiveness task made the strongest contribution to team performance. Managing emotions made a significant negative contribution to team performance.
A Monthly Double-blind Peer Reviewed Refereed Open Access International E-journal -included in the International Serial Directories Ownership Structure and Oppurtunistic Accounting: a Case of Listed Food and Beverage Firms in Nigeria _________________________________________________________ a Monthl

Earnings management has been identified by regulators and practitioners as a tool used by managers to mislead investors about the underlying economic conditions of their firms. Although, the relationship between ownership structure and the discretionery behaviour of managers has been considerably discussed in developed countries, an examination into this relationship has only recently attracted the attention of researchers in the developing economies such as Nigeria. This paper examines the impact of ownership structure on earnings management in quoted food and beverage firms in Nigeria. Secondary data were extracted from the annual reports of our sample firms for the period between 2006 to 2010 and OLS multiple regression is used as a tool for data analysis. The result indicates that ownership structure affects earnings management in divergent ways. Specifically, the study documents an inverse relationship between institutional sherholding and discretionery accruals. While ownership concentration and family ownership positively impact on earnings manipulation.
The fragile X mental retardation syndrome protein interacts with novel homologs FXR1 and FXR2.

Fragile X Mental Retardation Syndrome is the most common form of hereditary mental retardation, and is caused by defects in the FMR1 gene. FMR1 is an RNA‐binding protein and the syndrome results from lack of expression of FMR1 or expression of a mutant protein that is impaired in RNA binding. The specific function of FMR1 is not known. As a step towards understanding the function of FMR1 we searched for proteins that interact with it in vivo. We have cloned and sequenced a protein that interacts tightly with FMR1 in vivo and in vitro. This novel protein, FXR2, is very similar to FMR1 (60% identity). FXR2 encodes a 74 kDa protein which, like FMR1, contains two KH domains, has the capacity to bind RNA and is localized to the cytoplasm. The FXR2 gene is located on human chromosome 17 at 17p13.1. In addition, FMR1 and FXR2 interact tightly with the recently described autosomal homolog FXR1. Each of these three proteins is capable of forming heteromers with the others, and each can also form homomers. FXR1 and FXR2 are thus likely to play important roles in the function of FMR1 and in the pathogenesis of the Fragile X Mental Retardation Syndrome.
A comparison of the chromosome G-banding pattern in two Sorex species, S. satunini and S. araneus (Mammalia, Insectivora)

Abstract The G-banded karyotype of Sorex satunini was compared with the karyotype of Sorex araneus. Extensive homology was revealed. The major chromosomal rearrangements involved in the evolutionary divergence of these species have been identified as centric fusions and centromeric shifts. From the known palaeontological age of Sorex satunini it is obvious that the vast chromosomal polymorphism of the Sorex araneus group originated during the middle Pleistocene.
Physical Orbit for λ Virginis and a Test of Stellar Evolution Models

The star λ Virginis is a well-known double-lined spectroscopic Am binary with the interesting property that both stars are very similar in abundance but one is sharp-lined and the other is broad-lined. We present combined interferometric and spectroscopic studies of λ Vir. The small scale of the λ Vir orbit (~20 mas) is well resolved by the Infrared Optical Telescope Array (IOTA), allowing us to determine its elements, as well as the physical properties of the components, to high accuracy. The masses of the two stars are determined to be 1.897 and 1.721 M☉, with 0.7% and 1.5% errors, respectively, and the two stars are found to have the same temperature of 8280 ± 200 K. The accurately determined properties of λ Vir allow comparisons between observations and current stellar evolution models, and reasonable matches are found. The best-fit stellar model gives λ Vir a subsolar metallicity of Z = 0.0097 and an age of 935 Myr. The orbital and physical parameters of λ Vir also allow us to study its tidal evolution timescales and status. Although atomic diffusion is currently considered to be the most plausible cause of the Am phenomenon, the issue is still being actively debated in the literature. With the present study of the properties and evolutionary status of λ Vir, this system is an ideal candidate for further detailed abundance analyses that might shed more light on the source of the chemical anomalies in these A stars.
Constrained maximization of saliency of intended object for guiding attention

Saliency of an object in an image determines the attentiveness of the object with respect to human visual system. Saliency, in the absence of any external stimuli, is determined by contrast of features (like intensity, color, etc.) of the object with its surroundings. This paper proposes a novel technique to enhance the saliency of an object, which is not intrinsically salient. In order to enhance the attentiveness, the feature values of a used-defined target object (whose saliency has to be enhanced) should have more differences with the feature values of its surrounding objects. But too much modification of these values of the target object will destroy the naturalness of the image. So this problem of enhancing saliency of a target object is treated as a maximization problem under some constraints.
Gain‐of‐Function of Olig Transcription Factors Enhances Oligodendrogenesis and Myelination

The basic helix‐loop‐helix transcription factors Olig1 and Olig2 are required for oligodendrocyte specification and differentiation during central nervous system (CNS) development but the effects of overexpression of these factors in murine development are not well understood. To test whether Olig1 and Olig2 may reprogram CNS stem/progenitors toward an oligodendroglial fate for myelination, we generated transgenic mice with doxycycline (Dox)‐inducible expression of Olig1 or Olig2 in nestin‐expressing stem/progenitor cells of the CNS. Overexpression of Olig1 or Olig2 from E8.5 to E12.5 was sufficient to promote the generation of Platelet‐Derived Growth Factor Receptor alpha + oligodendrocyte precursors (OPCs) in the spinal cord. We also demonstrated that overexpression of Olig2, but not Olig1, enhanced the stem/progenitor cell proliferation and generation of motoneuron precursors and inhibited the development of V3 interneurons. In the postnatal brain, Dox‐inducible expression of Olig2 but not Olig1 in nestin+ stem/progenitors of the subventricular zone increased the generation of OPCs that migrated and differentiated into mature oligodendrocytes in the corpus callosum, cortex and olfactory bulb, leading to increased and precocious myelination. Altogether, our data indicate that Olig2 is a potential therapeutic target to enhance myelination and remyelination in the CNS. STEM CELLS 2010; 28:1611–1622.
A Novel Role of CDX1 in Embryonic Epicardial Development

The molecular mechanism that regulates epicardial development has yet to be understood. In this study, we explored the function of CDX1, a Caudal-related family member, in epicardial epithelial-to-mesenchymal transition (EMT) and in the migration and the differentiation of epicardium-derived progenitors into vascular smooth muscle cells. We detected a transient expression of CDX1 in murine embryonic hearts at 11.5 days post coitum (dpc). Using a doxycycline-inducible CDX1 mouse model, primary epicardium, and ex vivo heart culture, we further demonstrated that ectopic expression of CDX1 promoted epicardial EMT. In addition, a low-dose CDX1 induction led to enhanced migration and differentiation of epicardium-derived cells into α-SMA+ vascular smooth muscles. In contrast, either continued high-level induction of CDX1 or CDX1 deficiency attenuated the ability of epicardium-derived cells to migrate and to mature into smooth muscles induced by TGF-β1. Further RNA-seq analyses showed that CDX1 induction altered the transcript levels of genes involved in neuronal development, angiogenesis, and cell adhesions required for EMT. Our data have revealed a previously undefined role of CDX1 during epicardial development, and suggest that transient expression of CDX1 promotes epicardial EMT, whereas subsequent down-regulation of CDX1 after 11.5 dpc in mice is necessary for further subepicardial invasion of EPDCs and contribution to coronary vascular endothelium or smooth muscle cells.
Differential Changes with Age in Multiscale Entropy of Electromyography Signals from Leg Muscles during Treadmill Walking

Age-related gait changes may be due to the loss of complexity in the neuromuscular system. This theory is disputed due to inconsistent results from single-scale analyses. Also, behavioral adaptations may confound these changes. We examined whether EMG dynamics during gait is less complex in older adults over a range of timescales using the multiscale entropy method, and whether slower walking attenuates this effect. Surface EMG was measured from the left vastus lateralis (VL), biceps femoris (BF), gastrocnemius (GA), and tibialis anterior (TA) in 17 young and 18 older adults as they walked on a treadmill for 5 minutes at 0.8x-1.2x of preferred speed. Sample entropy (SE) and the complexity index (CI) of the EMG signals were calculated after successive coarse-graining to extract dynamics at timescales of 27 to 270 Hz, with m = 2 and r = 0.15 SD. SE and CI were lower across the timescales in older adults in VL and BF, but higher in GA (all p<0.001); these results held for VL and GA even after accounting for longer EMG burst durations in older adults. CI was higher during slower walking speed in VL and BF (p<0.001). Results were mostly similar for m = 3 and r = 0.01–0.35. Smaller r was more sensitive to age-related differences. The decrease in complexity with aging in the timescales studied was limited to proximal muscles, particularly VL. The increase in GA may be driven by other factors. Walking slower may reflect a behavioral adaptation that allows the nervous system to function with greater complexity.
Exploring Mechanisms of Effective Teacher Coaching

Although previous research has shown that teacher coaching can improve teaching practices and student achievement, little is known about specific features of effective coaching programs. We estimate the impact of MATCH Teacher Coaching (MTC) on a range of teacher practices using a blocked randomized trial and explore how changes in the coaching model across two cohorts are related to program effects. Findings indicate large positive effects in Cohort 1 but no effects in Cohort 2. After ruling out explanations related to the research design, a set of exploratory analyses suggest that differential treatment effects may be attributable to differences in coach effectiveness, coaching dosage, and the focus of coaching across cohorts.