Miracle-dz/UniFAR-FTUs huggingface dataset:
Exposure to Bisphenol A Correlates with Early-Onset Prostate Cancer and Promotes Centrosome Amplification and Anchorage-Independent Growth In Vitro

Human exposure to bisphenol A (BPA) is ubiquitous. Animal studies found that BPA contributes to development of prostate cancer, but human data are scarce. Our study examined the association between urinary BPA levels and Prostate cancer and assessed the effects of BPA on induction of centrosome abnormalities as an underlying mechanism promoting prostate carcinogenesis. The study, involving 60 urology patients, found higher levels of urinary BPA (creatinine-adjusted) in Prostate cancer patients (5.74 µg/g [95% CI; 2.63, 12.51]) than in non-Prostate cancer patients (1.43 µg/g [95% CI; 0.70, 2.88]) (p = 0.012). The difference was even more significant in patients <65 years old. A trend toward a negative association between urinary BPA and serum PSA was observed in Prostate cancer patients but not in non-Prostate cancer patients. In vitro studies examined centrosomal abnormalities, microtubule nucleation, and anchorage-independent growth in four Prostate cancer cell lines (LNCaP, C4-2, 22Rv1, PC-3) and two immortalized normal prostate epithelial cell lines (NPrEC and RWPE-1). Exposure to low doses (0.01–100 nM) of BPA increased the percentage of cells with centrosome amplification two- to eight-fold. Dose responses either peaked or reached the plateaus with 0.1 nM BPA exposure. This low dose also promoted microtubule nucleation and regrowth at centrosomes in RWPE-1 and enhanced anchorage-independent growth in C4-2. These findings suggest that urinary BPA level is an independent prognostic marker in Prostate cancer and that BPA exposure may lower serum PSA levels in Prostate cancer patients. Moreover, disruption of the centrosome duplication cycle by low-dose BPA may contribute to neoplastic transformation of the prostate.
Novel cell separation method for molecular analysis of neuron-astrocyte co-cultures

Over the last decade, the importance of astrocyte-neuron communication in neuronal development and synaptic plasticity has become increasingly clear. Since neuron-astrocyte interactions represent highly dynamic and reciprocal processes, we hypothesized that many astrocyte genes may be regulated as a consequence of their interactions with maturing neurons. In order to identify such neuron-responsive astrocyte genes in vitro, we sought to establish an expedited technique for separation of neurons from co-cultured astrocytes. Our newly established method makes use of cold jet, which exploits different adhesion characteristics of subpopulations of cells (Jirsova etal., 1997), and is rapid, performed under ice-cold conditions and avoids protease-mediated isolation of astrocytes or time-consuming centrifugation, yielding intact astrocyte mRNA with approximately 90% of neuronal RNA removed. Using this purification method, we executed genome-wide profiling in which RNA derived from astrocyte-only cultures was compared with astrocyte RNA derived from differentiating neuron-astrocyte co-cultures. Data analysis determined that many astrocytic mRNAs and biological processes are regulated by neuronal interaction. Our results validate the cold jet as an efficient method to separate astrocytes from neurons in co-culture, and reveals that neurons induce robust gene-expression changes in co-cultured astrocytes.
Evaluation of an Education and Training Program to Prevent and Manage Patients’ Violence in a Mental Health Setting: A Pretest-Posttest Intervention Study

Workplace violence can lead to serious consequences for victims, organizations, and society. Most workplace violence prevention programs aim to train staff to better recognize and safely manage at-risk situations. The Omega education and training program was developed in Canada in 1999, and has since been used to teach healthcare and mental health workers the skills needed to effectively intervene in situations of aggression. The present study was designed to assess the impact of Omega on employee psychological distress, confidence in coping, and perceived exposure to violence. This program was offered to 105 employees in a psychiatric hospital in Montreal, Canada. Eighty-nine of them accepted to participate. Questionnaires were completed before the training, after a short period of time (M = 109 days) and at follow-up (M = 441 days). Repeated-measures ANOVAs and Cohen’s d effect sizes were calculated. Results demonstrated statistically significant improvements in short-term and follow-up posttest scores of psychological distress, confidence in coping, and in levels of exposure to violence. This study is one of very few to demonstrate the positive impact of this training program. Further research is needed to understand how to improve the effectiveness of the program, especially among participants resistant to change.
The Scale Structure of Multi-Scale Measures: Application of the Split-Scale Method to the Task- Specific Occupational Self-Efficacy Scale and the Career Decision-Making Self-Efficacy Scale

The present study evaluated the internal structure of two translated multiple-scale questionnaires: the Task-Specific Occupational Self-Efficacy Scale (TSOSS; Osipow, Temple, & Rooney, 1993) and the Career Decision-Making Self-Efficacy Scale (CDMSE; Taylor & Betz, 1983). Data were obtained during a pilot study for testing the Hebrew versions of the TSOSS and the CDMSE. Cluster analysis applied to the split scales was found to complement the traditional method used to assess the psychometric properties of the instruments. As proposed by its authors, the TSOSS was found to possess four distinct scales in both language versions. The elimination of problematic items from the Hebrew version revealed the five hypothesized factors assumed to underlie the CDMSE. The proposed clustering analysis of the split scales appears to have potential application to the refinement process of other multi-scale instruments as well.
Effects of Playing Surface (Hard and Clay Courts) on Heart Rate and Blood Lactate During Tennis Matches Played by High-Level Players

Martin, C, Thevenet, D, Zouhal, H, Mornet, Y, Delès, R, Crestel, T, Ben Abderrahman, A, and Prioux, J. Effects of playing surface (hard and clay courts) on heart rate and blood lactate during tennis matches played by high level players. J Strength Cond Res 25(1): 163-170, 2011-The aim of this study was to compare tennis matches played on clay (CL) and resin (R) courts. Six matches were played (3 on CL courts and 3 on R courts) by 6 high-level players. Heart rate (HR) was monitored continuously while running time (4.66 m), and blood lactate concentration ([La]) were measured every 4 games. Mean duration of points and effective playing time (EPT) were measured for each match. Mean HR (154 ± 12 vs. 141 ± 9 b·min−1) and [La] values (5.7 ± 1.8 vs. 3.6 ± 1.2 mmol·L−1) were significantly higher on CL (p < 0.05). The [La] increased significantly during the match on CL court. Mean duration of rallies (8.5 ± 0.2 vs. 5.9 ± 0.5 seconds) and EPT (26.2 ± 1.9 vs. 19.5 ± 2.0%) were significantly longer (p < 0.05) on CL. Running time values in speed tests were not significantly different between CL and R. Running time performance was not significantly decreased during the match, whatever the playing surface. This study shows that the court surface influences the characteristics of the match and the player's physiological responses. The court surface should be a key factor for consideration when coaches determine specific training programs for high-level tennis players.
Hierarchical Cheap Talk

We investigate situations in which agents can communicate to each other only through a chain of intermediators, for example because they have to obey institutionalized communication protocols. We assume that all involved in the communication are strategic, and might want to influence the action taken by the final receiver. The set of pure strategy equilibrium outcomes is simple to characterize, monotonic in each intermediator's bias, does not depend on the order of intermediators, and intermediation in these equilibria cannot improve information transmission. However, none of these conclusions hold for mixed equilibria. We provide a partial characterization of mixed equilibria, and offer an economically relevant sufficient condition for every equilibrium to be outcome-equivalent to a pure equilibrium and hence the simple characterization and comparative statics results to hold for the set of all equilibria.
Noncompliance and child-rearing attitudes as predictors of aggressive behaviour: A longitudinal study in Chinese children

The purpose of the study was to examine contributions of early personal and socialisation factors to the prediction of aggressive behaviour in Chinese children. A sample of children, initially at 2 years of age, and their parents in the People's Republic of China participated in this two-year longitudinal study. Observational data were collected on children's noncompliance in mother-child interactions at Time 1 and aggressive behaviour in peer interactions at Time 2. In addition, information concerning child-rearing attitudes were obtained from parental reports. The results indicated that boys had higher scores than girls on physical and verbal aggression at 4 years of age. Early noncompliance significantly and positively predicted aggressive behaviour. Parental child-rearing attitudes had unique, but generally weak, contributions to the prediction of aggression. Finally, the relations between child-rearing variables and child aggression might be different for boys and girls.
Output consensus of nonlinear multi-agent systems with unknown control directions

In this paper, we consider an output consensus problem for a general class of nonlinear multi-agent systems without a prior knowledge of the agents' control directions. Two distributed Nussbaumtype control laws are proposed to solve the leaderless and leader-following adaptive consensus for heterogeneous multiple agents. Examples and simulations are given to verify their effectiveness
Extracellular ATP or ADP Induce Chemotaxis of Cultured Microglia through Gi/o-Coupled P2Y Receptors

The initial microglial responses that occur after brain injury and in various neurological diseases are characterized by microglial accumulation in the affected sites of brain that results from the migration and proliferation of these cells. The early-phase signal responsible for this accumulation is likely to be transduced by rapidly diffusible factors. In this study, the possibility of ATP released from injured neurons and nerve terminals affecting cell motility was determined in rat primary cultured microglia. Extracellular ATP and ADP induced membrane ruffling and markedly enhanced chemokinesis in Boyden chamber assay. Further analyses using the Dunn chemotaxis chamber assay, which allows direct observation of cell movement, revealed that both ATP and ADP induced chemotaxis of microglia. The elimination of extracellular calcium or treatment with pyridoxalphosphate-6-azophenyl-2′,4′-disulphonic acid, suramin, or adenosine-3′-phosphate-5′-phosphosulfate did not inhibit ATP- or ADP-induced membrane ruffling, whereas AR-C69931MX or pertussis toxin treatments clearly did so. As an intracellular signaling molecule underlying these phenomena, the small G-protein Rac was activated by ATP and ADP stimulation, and its activation was also inhibited by pretreatment with pertussis toxin. These results strongly suggest that membrane ruffling and chemotaxis of microglia induced by ATP or ADP are mediated by Gi/o-coupled P2Y receptors.
THE ORIGIN OF SERUM PROTEIN, A, B AND H BLOOD GROUP, AND Gm AND Inv ANTIGENS IN HOUSE DUST

Materials precipitated from an aqueous extract of house dust by saturation with ammonium sulphate showed immunological reaction with antisera to human serum albumin, human alpha1-acid glycoprotein, human IgG, Gm and Inv antigens and to A, B and H antigens. It is concluded that the albumin and alpha1-acid glycoprotein are of human origin. It seems that the apparent IgG activity is due to cross-reactivity since, from the anticipated specificities of the Fab and Fc fragments of human IgG, the former could not be detected, and since Gm (6) activity was present, unexpectedly, in Swiss house dust. The A, B and H blood group antigenic activities were detected in relative concentration different from those expected if they were solely of human origin. It is concluded that they are from both human and non-human sources.
Cytokines and growth factors in keratinocytes and sweat glands in chronic venous leg ulcers. An immunohistochemical study

The role of growth factors and cytokines in the impaired healing of chronic leg ulcers remains uncertain. The aim of this study was to determine whether changes in the amount and location of cytokines and growth factors may be associated with impaired healing in chronic leg ulcers. Biopsies from leg ulcers of 21 patients and from normal skin of nine healthy volunteers were examined immunohistochemically for selected growth factors and cytokines. Greater staining intensity was found in keratinocytes at the edges of ulcers compared to normal skin, or skin adjacent to the ulcers. Staining at the ulcer edge was more intense in nonhealing ulcers for only vascular endothelial growth factor and platelet‐derived growth factor, whereas staining in the adjacent skin was more intense for all factors in the nonhealing phase. For all factors staining was cytoplasmic, suggesting production in these areas. This study shows up‐regulation of the production of cytokines and growth factors in keratinocytes of chronic leg ulcers that is greater when the ulcers are nonhealing. (WOUND REP REG 2003;11:316–325)
Regionalized Sequence of Myocardial Cell Growth and Proliferation Characterizes Early Chamber Formation

Increase in cell size and proliferation of myocytes are key processes in cardiac morphogenesis, yet their regionalization during development of the heart has been described only anecdotally. We have made quantitative reconstructions of embryonic chicken hearts ranging in stage from the fusion of the heart-forming fields to early formation of the chambers. These reconstructions reveal that the early heart tube is recruited from a pool of rapidly proliferating cardiac precursor cells. The proliferation of these small precursor cells ceases as they differentiate into overt cardiomyocytes, producing a slowly proliferating straight heart tube composed of cells increasing in size. The largest cells were found at the ventral side of the heart tube, which corresponds to the site of the forming ventricle, as well as the site where proliferation is reinitiated. The significance of these observations is 2-fold. First, they support a model of early cardiac morphogenesis in 2 stages. Second, they demonstrate that regional increase in size of myocytes contributes significantly to chamber formation.
Antihyperlipidemic Properties of Novel N-(Benzoylphenyl)-5-substituted-1H-indole-2-carboxamides in Triton WR-1339-Induced Hyperlipidemic Rats

In the search for new potential antihyperlipidemic agents, the present study focuses on the synthesis of novel N-(benzoylphenyl)-5-substituted-1H-indole-2-carboxamides (compounds 8-12, 15, 16, 18) and investigating their antihyperlipidemic activity using Triton WR-1339-induced hyperlipidemic rats as an experimental model. Hyperlipidemia was developed by intraperitoneal injection of Triton WR-1339 (250 mg/kg body weight). The tested animals were divided into normal control (NCG), hyperlipidemic (HG), compound 8, 9, 15, 16, 18- and bezafibrate treated groups. At a dose of 15 mg/kg body weight, compounds 9, 16, 18 and bezafibrate (100 mg/kg) significantly (p < 0.0001) reduced elevated plasma triglycerides levels after 12 h compared to the hyperlipidemic control group. However, only the group treated with compounds 9, 16 and 18 showed an obviously significant (p < 0.001) reduction in plasma total cholesterol levels after 12 h compared to the hyperlipidemic control group. Moreover, high density lipoprotein-cholesterol levels were significantly (p < 0.0001) increased in all treated groups after 12 h compared to the hyperlipidemic control group, except for compounds 8 and 15 which revealed inactive. It is therefore reasonable to assume that compounds 9, 16 and 18 may have potential in the treatment of hyperlipidemia.
Trefoil factor 1 expression suppresses Helicobacter pylori–induced inflammation in gastric carcinogenesis

Infection with Helicobacter pylori, a high‐risk factor for gastric cancer, is frequently associated with chronic inflammation through activation of nuclear factor κB (NF‐κB). Trefoil factor 1 (TFF1) is a constitutively expressed protein in the stomach that has tumor‐suppressor functions and plays a critical role in maintaining mucosal integrity. This study investigated the role of TFF1 in regulating the proinflammatory response to H. pylori infections.
When problem solving is superior to studying worked examples.

Interactions between levels of learner knowledge in a domain and levels of instructional guidance were investigated. Inexperienced mechanical trade apprentices were presented with either a series of worked examples to study or problems to solve. On subsequent tests, inexperienced trainees benefited most from the worked examples condition, with this group performing better with lower ratings of mental load than similar trainees who solved problems. With more experience in the domain, worked examples became redundant and problem solving proved superior. It is suggested that the relative effectiveness of either worked examples or problem solving depends heavily on levels of learner knowledge.
Human resource practices, organizational climate, and customer satisfaction

The degree to which organizational climate mediates the relationship between human resource practices and customer satisfaction is investigated for 351 small businesses in the same industry. Results indicated support for the hypothesized mediated relationship. The indirect effects of HR practices on customer satisfaction were significant and relatively large while the direct effect was nonsignificant and near zero. The results were supportive of a social context model of the impact of human resource practices on organizational outcomes. Limitations of the study and implications for future research are discussed.
The Legend of "Nigger" Lake

The term scapegoating has had three principal meanings that have not always been clearly differentiated: biblical, anthropological, and psychosocial. Originally, the scapegoat was a term used to refer to the first of two goats in the Leviticus ritual, the one chosen to carry (symbolically) the sins of the Hebrews into exile. In the 19th century, anthropologists generalized from the biblical ritual and applied the term to a wide range of rituals, which later became a subcategory called scapegoat rituals or rites for the expulsion of evil. For at least 20 years, however, the anthropological use of this category has been moribund. Finally, in everyday discourse, in journalism, and in popular literature, scapegoat is a term used to refer to those unfairly blamed for problems of any kind. This general psychosocial meaning has become predominate (Girard, 1987a, pp. 73-74). Despite the fact that the psychosocial meaning of scapegoat is widespread, the term has been given short shrift in the social sciences. Two approaches characterize those who do not use scapegoat in an exclusively descriptive way. Some social scientists, often
The sustainability of corporate wikis: A time-series analysis of activity patterns

While existing theoretical frameworks describe collective technology adoption patterns, they provide little insight regarding the expected patterns of wiki activity within projects. Another impediment to the study of wiki sustainability is the absence of time-series analysis methods that are suitable for the unique patterns of wiki activity logs. The primary goals of this study are to: (i) develop a novel method for analyzing wiki edit activity logs, (ii) reveal the temporal patterns of corporate wiki edit activity, and (iii) study the factors impacting wikis' sustainability. A validation of our proposed method demonstrates that it is superior to the baseline algorithm in the face of noisy data. Our empirical study combines wiki system edit activity logs with a survey of users' perceptions, and explores 33277 distinct wiki applications within one global organization over the first 5 years of wiki operation. Our results reveal six different prototypical wiki activity patterns, and show that most corporate wikis become inactive after a relatively short period. Findings from the user survey show that users of sustainable wikis are more satisfied with the wiki system and its contents, and feel that the wiki provides them with a sense of community and productivity enhancements.
The Sleep Disturbance Scale for Children (SDSC) Construct ion and validation of an instrument to evaluate sleep disturbances in childhood and adolescence

SUMMARY To attempt a categorization of sleep disorders in children, we developed a 27 item Likert‐type rating scale (Sleep Disturbance Scale for Children: SDSC) and assessed the psychometric properties was developed. The scale was distributed to the mothers of 1304 children (1157 controls, mean age 9.8y; 147 sleep disorder subjects, mean age 9.2y, composed of four clinical groups: Insomnia 39 subjects, Hypersomnia 12 subjects, Respiratory disturbances during sleep 25 subjects and Parasomnias 71 subjects). The internal consistency was high in controls (0.79) and remained at a satisfactory level in sleep disorder subjects (0.71); the test/retest reliability was adequate for the total (r = 0.71) and single item scores. The factor analysis (variance explained 44.21%) yielded six factors which represented the most common areas of sleep disorders in childhood and adolescence. Enuresis was the only item with a factor loading lower than 0.40 and with a low inter‐item correlation and was therefore eliminated, resulting in a final scale of 26 items. The re‐evaluation of the sample, using the factor scores, supported the validity and the discriminating capacity of the scales between controls and the four clinical groups. The correlation between factor scores corroborated the hypothesis that childhood sleep disturbances are not independent entities nor do they cluster into different groupings related to each other. The SDSC appears to be a useful tool in evaluating the sleep disturbances of school‐age children in clinical and non‐clinical populations.
Complexity Driven Recombination of MIDI Loops

An algorithm and a software application for recombining in real time MIDI drum loops that makes use of a novel analysis of rhythmic patterns that sorts them in order of their complexity is presented. We measure rhythmic complexity by comparing each rhythmic pattern found in the loops to a metrical template characteristic of its time signature. The complexity measure is used to sort the MIDI loops prior to utilizing them in the recombination algorithm. This way, the user can effectively control the complexity and variation in the generated rhythm during performance.
What Makes a Good Elementary School? A Critical Examination

Abstract With a renewed emphasis on student achievement, school improvement, and shared decision making, and in an effort to create and maintain effective schools, researchers try to delineate those characteristics that positively affect student learning and behavior. In exploring the different views that persons have regarding the characteristics of effective schools, the authors reviewed research studies to identify factors considered to be determinants of an effective school. Specific topic areas examined were the views of teachers, parents, and administrators regarding effective schools, as well as an examination of the related research literature. Consistent across the 4 areas was the importance of a positive school climate and strong leadership. Implications for educational leaders are provided.