Miracle-dz/UniFAR-FTUs huggingface dataset:
A smac mimetic reduces TNF Related Apoptosis Inducing Ligand (TRAIL)‐induced invasion and metastasis of cholangiocarcinoma cells

Cholangiocarcinoma (CCA) cells paradoxically express tumor necrosis factor–related apoptosis‐inducing ligand (TRAIL), a death ligand that, failing to kill CCA cells, instead promotes their tumorigenicity and especially the metastatic behaviors of cell migration and invasion. Second mitochondria‐derived activator of caspase (smac) mimetics are promising cancer therapeutic agents that enhance proapoptotic death receptor signaling by causing cellular degradation of inhibitor of apoptosis (IAP) proteins. Our aim was to examine the in vitro and in vivo effects of the smac mimetic JP1584 in CCA. Despite JP1584‐mediated loss of cellular inhibitor of apoptosis‐1 (cIAP‐1) and cIAP‐2, TRAIL failed to induce apoptosis in KMCH‐1, TFK‐1, and BDEneu CCA cells; a finding consistent with a downstream block in death signaling. Because cIAP‐1 and cIAP‐2 also promote nuclear factor kappa B (NF‐κB) activation by the canonical pathway, the effect of JP1584 on this signaling pathway was examined. Treatment with JP1584 inhibited TRAIL‐induced NF‐κB activation as well as TRAIL‐mediated up‐regulation of the NF‐κB target gene, matrix metalloproteinase 7 (MMP7). JP1584 also reduced TRAIL‐mediated CCA cell migration and invasion in vitro. Finally, in a syngeneic rat orthotopic CCA model, JP1584 administration reduced MMP7 messenger RNA levels and extrahepatic metastases.
A SON solution for sleeping cell detection using low-dimensional embedding of MDT measurements

Automatic detection of cells which are in outage has been identified as one of the key use cases for Self Organizing Networks (SON) for emerging and future generations of cellular systems. A special case of cell outage, referred to as Sleeping Cell (SC) remains particularly challenging to detect in state of the art SON because in this case cell goes into outage or may perform poorly without triggering an alarm for Operation and Maintenance (O&M) entity. Consequently, no SON compensation function can be launched unless SC situation is detected via drive tests or through complaints registered by the affected customers. In this paper, we present a novel solution to address this problem that makes use of minimization of drive test (MDT) measurements recently standardized by 3GPP and NGMN. To overcome the processing complexity challenge, the MDT measurements are projected to a low-dimensional space using multidimensional scaling method. Then we apply state of the art k-nearest neighbor and local outlier factor based anomaly detection models together with pre-processed MDT measurements to profile the network behaviour and to detect SC. Our numerical results show that our proposed solution can automate the SC detection process with 93% accuracy.
Incidence and Characterization of Resistance to Fenpropathrin in Some Liriomyza sativae (Diptera: Agromyzidae) Populations in Iran

ABSTRACT The vegetable leafminer, Liriomyza sativae Blanchard, is a key pest of vegetable and ornamental crops worldwide and is mainly controlled by insecticides. The toxicity of fenpropathrin on adult flies of F1 generation of six Iranian populations of the pest was assessed using the residual glass vial method. The bioassay results showed significant differences in susceptibility to fenpropathrin among the populations tested. Resistance ratios ranged from 5.09 to 28.32. The resistant populations were reared in the greenhouse under fenpropathrin pressure for 14 mo and then susceptibility of both larvae and adults of these strains to fenpropathrin was evaluated. The resistance levels significantly increased in these populations. Further study demonstrated that diethyl maleate and triphenyl phosphate had no synergistic effects on fenpropathrin, however, this compound was significantly synergized by piperonyl butoxide in all tested populations. S,S,S-tributyl phosphorotrithioate also showed a slight synergism with fenpropathrin. Enzyme assays for general esterases, glutathione S-transferases and monooxygenases were performed on adults and larvae of L. sativae. The activity of glutathione s-transferase in different resistant and susceptible strains was not significantly different. The activity of esterases in resistant strains was higher than the susceptible strain. Monooxygenase levels also were higher in resistant strains compared with the susceptible strain. Synergism and biochemical assays revealed that esterases and monooxygenases have a major role in resistance of L. sativae to fenpropathrin.
F-18 Fluorodeoxyglucose PET/CT and Post Hoc PET/MRI in a Case of Primary Meningeal Melanomatosis

Primary meningeal melanomatosis is a rare, aggressive variant of primary malignant melanoma of the central nervous system, which arises from melanocytes within the leptomeninges and carries a poor prognosis. We report a case of primary meningeal melanomatosis in a 17-year-old man, which was diagnosed with 18F-fluorodeoxyglucose (F-18 FDG) PET/CT, and post hoc F-18 FDG PET/MRI fusion images. Whole-body F-18 FDG PET/CT was helpful in ruling out the extracranial origin of melanoma lesions, and in assessing the therapeutic response. Post hoc PET/MRI fusion images facilitated the correlation between PET and MRI images and demonstrated the hypermetabolic lesions more accurately than the unenhanced PET/CT images. Whole body F-18 FDG PET/CT and post hoc PET/MRI images might help clinicians determine the best therapeutic strategy for patients with primary meningeal melanomatosis.
HDAC6-mediated acetylation of lipid droplet–binding protein CIDEC regulates fat-induced lipid storage

Obesity is characterized by aberrant fat accumulation. However, the intracellular signaling pathway that senses dietary fat and leads to fat storage remains elusive. Here, we have observed that the levels of histone deacetylase 6 (HDAC6) and the related family member HDAC10 are markedly reduced in adipose tissues of obese animals and humans. Mice with adipocyte-specific depletion of Hdac6 exhibited increased fat accumulation and reduced insulin sensitivity. In normal adipocytes, we found that reversal of P300/CBP-associated factor–induced (PCAF-induced) acetylation at K56 on cell death-inducing DFFA-like effector C (CIDEC, also known as FSP27) critically regulated lipid droplet fusion and lipid storage. Importantly, HDAC6 deacetylates CIDEC, leading to destabilization and reduced lipid droplet fusion. Accordingly, we observed elevated levels of CIDEC and its acetylated form in HDAC-deficient adipocytes as well as the adipose tissue of obese animals and humans. Fatty acids (FAs) prevented CIDEC deacetylation by promoting the dissociation of CIDEC from HDAC6, which resulted in increased association of CIDEC with PCAF on the endoplasmic reticulum. Control of CIDEC acetylation required the conversion of FAs to triacylglycerols. Thus, we have revealed a signaling axis that is involved in the coordination of nutrient availability, protein acetylation, and cellular lipid metabolic responses.
The diversity of floral temperature patterns, and their use by pollinators

Pollinating insects utilise various sensory cues to identify and learn rewarding flower species. One such cue is floral temperature, created by captured sunlight or plant thermogenesis. Bumblebees, honeybees and stingless bees can distinguish flowers based on differences in overall temperature between flowers. We report here that floral temperature often differs between different parts of the flower creating a temperature structure or pattern. Temperature patterns are common, with 55% of 118 plant species thermographed, showing within-flower temperature differences greater than the 2°C difference that bees are known to be able to detect. Using differential conditioning techniques, we show that bumblebees can distinguish artificial flowers differing in temperature patterns comparable to those seen in real flowers. Thus, bumblebees are able to perceive the shape of these within-flower temperature patterns. Floral temperature patterns may therefore represent a new floral cue that could assist pollinators in the recognition and learning of rewarding flowers.
Efficacy of supplemental microbial phytase at different dietary calcium levels on growth performance and mineral utilization of broiler chickens.

A 3-wk feeding trial with 240 sexed, day-old broiler chickens was conducted to determine the efficacy of microbial phytase at different levels of dietary Ca on performance and utilization of minerals in broiler chickens fed a low-P corn-soybean diet. The experimental design was a 3 x 2 factorial arrangement of treatments; Ca at 0.6, 1.0, and 1.25% and phytase at 0 and 600 phytase U/kg diet. Phytase supplementation, regardless of Ca level, increased (P < or = 0.005) feed intake, (P < or = 0.0001) body weight, and (P < or = 0.025) feed efficiency at 21 d; the optimum levels of body weight, feed intake, and feed efficiency were obtained with low (0.6%) dietary Ca plus phytase. Retentions of P, Ca, and N were increased (P < or = 0.05) by phytase supplementation. Although maximum retentions of P and N were obtained at the 1.0 and 1.25% Ca levels, respectively, they were not significantly different from the values obtained at 0.6% Ca. The increasing level of dietary Ca decreased plasma P ( P < or = 0.05) and Cu (P < or = 0.06). Phytase supplementation had the opposite effect; i.e., increased plasma P (P < or = 0.03) and Cu (P < or = 0.02). The maximum level of plasma P was obtained with phytase at the 1.0% Ca level, but this value was not significantly different from the value obtained with phytase at the 0.6% Ca level. Phytase supplementation increased (P < 0.04) the ash content of both tibia head and shaft but had no effect on mineral contents in the ash. The optimum level of ash content was observed with the 0.06% Ca diet plus phytase. The results show that microbial phytase supplementation to a low P diet improved growth performance and mineral utilization in broiler chickens. Dietary Ca levels had a significant effect on the response to phytase; the optimum growth performance and mineral utilization were achieved at the low (0.6%) level of dietary Ca supplemented with phytase.
Dissociative identity disorder: An empirical overview

Objective: Despite its long and auspicious place in the history of psychiatry, dissociative identity disorder (DID) has been associated with controversy. This paper aims to examine the empirical data related to DID and outline the contextual challenges to its scientific investigation. Methods: The overview is limited to DID-specific research in which one or more of the following conditions are met: (i) a sample of participants with DID was systematically investigated, (ii) psychometrically-sound measures were utilised, (iii) comparisons were made with other samples, (iv) DID was differentiated from other disorders, including other dissociative disorders, (v) extraneous variables were controlled or (vi) DID diagnosis was confirmed. Following an examination of challenges to research, data are organised around the validity and phenomenology of DID, its aetiology and epidemiology, the neurobiological and cognitive correlates of the disorder, and finally its treatment. Results: DID was found to be a complex yet valid disorder across a range of markers. It can be accurately discriminated from other disorders, especially when structured diagnostic interviews assess identity alterations and amnesia. DID is aetiologically associated with a complex combination of developmental and cultural factors, including severe childhood relational trauma. The prevalence of DID appears highest in emergency psychiatric settings and affects approximately 1% of the general population. Psychobiological studies are beginning to identify clear correlates of DID associated with diverse brain areas and cognitive functions. They are also providing an understanding of the potential metacognitive origins of amnesia. Phase-oriented empirically-guided treatments are emerging for DID. Conclusions: The empirical literature on DID is accumulating, although some areas remain under-investigated. Existing data show DID as a complex, valid and not uncommon disorder, associated with developmental and cultural variables, that is amenable to psychotherapeutic intervention.
Improving the Robustness of Electromyogram-Pattern Recognition for Prosthetic Control by a Postprocessing Strategy

Electromyogram (EMG) contains rich information for motion decoding. As one of its major applications, EMG-pattern recognition (PR)-based control of prostheses has been proposed and investigated in the field of rehabilitation robotics for decades. These prostheses can offer a higher level of dexterity compared to the commercially available ones. However, limited progress has been made toward clinical application of EMG-PR-based prostheses, due to their unsatisfactory robustness against various interferences during daily use. These interferences may lead to misclassifications of motion intentions, which damage the control performance of EMG-PR-based prostheses. A number of studies have applied methods that undergo a postprocessing stage to determine the current motion outputs, based on previous outputs or other information, which have proved effective in reducing erroneous outputs. In this study, we proposed a postprocessing strategy that locks the outputs during the constant contraction to block out occasional misclassifications, upon detecting the motion onset using a threshold. The strategy was investigated using three different motion onset detectors, namely mean absolute value, Teager–Kaiser energy operator, or mechanomyogram (MMG). Our results indicate that the proposed strategy could suppress erroneous outputs, during rest and constant contractions in particular. In addition, with MMG as the motion onset detector, the strategy was found to produce the most significant improvement in the performance, reducing the total errors up to around 50% (from 22.9 to 11.5%) in comparison to the original classification output in the online test, and it is the most robust against threshold value changes. We speculate that motion onset detectors that are both smooth and responsive would further enhance the efficacy of the proposed postprocessing strategy, which would facilitate the clinical application of EMG-PR-based prosthetic control.
RESPONSE OF YIELD AND YIELD COMPONENTS OF WHEAT TOWARDS FOLIAR SPRAY OF NITROGEN, POTASSIUM AND ZINC

An experimental trail was designed to quantify the response of yield and yield component of wheat toward foliar spray of nitrogen, potassium and zinc. The experiment was conducted at Agricultural Research Farm of Khyber Pakhtunkhwa Agricultural University, Peshawar during 2002-2003. Yield and yield component of wheat showed significant response towards foliar spray of Nitrogen, Potassium and Zinc. Maximum biological yield (8999 kg ha -1 ), number of grains (52) spike -1 and straw yield (6074 kg ha -1 ) were produced in plots under the effect of foliar spray of 0.5% N + 0.5% K + 0.5% Zn solution (once), while control (no spray) plots produced minimum biological yield (5447 kg ha -1 ), number of grains (29) spike -1 and straw yield (3997 kg ha -1 ). Similarly maximum thousand grain weight (46 g) and grain yield (2950 kg ha -1 ) were recorded in plots sprayed with 0.5% N + 0.5% K + 0.5% Zn solution (twice), followed by lowest values (36 g) and (1450 kg ha -1 ) in plots having no spray (control). Among the treatment of 0.5% N + 0.5% K + 0.5% Zn solution applied either one or two times, gave best response towards yield and yield components of wheat in irrigated area of Peshawar valley.
Testing nonlocal models of electron thermal conduction for magnetic and inertial confinement fusion applications

Three models for nonlocal electron thermal transport are here compared against Vlasov-Fokker-Planck (VFP) codes to assess their accuracy in situations relevant to both inertial fusion hohlraums and tokamak scrape-off layers. The models tested are (i) a moment-based approach using an eigenvector integral closure (EIC) originally developed by Ji, Held, and Sovinec [Phys. Plasmas 16, 022312 (2009)]; (ii) the non-Fourier Landau-fluid (NFLF) model of Dimits, Joseph, and Umansky [Phys. Plasmas 21, 055907 (2014)]; and (iii) Schurtz, Nicolai, and Busquet's [Phys. Plasmas 7, 4238 (2000)] multigroup diffusion model (SNB). We find that while the EIC and NFLF models accurately predict the damping rate of a small-amplitude temperature perturbation (within 10% at moderate collisionalities), they overestimate the peak heat flow by as much as 35% and do not predict preheat in the more relevant case where there is a large temperature difference. The SNB model, however, agrees better with VFP results for the latter problem if care is taken with the definition of the mean free path. Additionally, we present for the first time a comparison of the SNB model against a VFP code for a hohlraum-relevant problem with inhomogeneous ionisation and show that the model overestimates the heat flow in the helium gas-fill by a factor of ? 2 despite predicting the peak heat flux to within 16%.
THE EFFECT OF CARBON SOURCE ON IN VITRO ORGANOGENESIS OF CHRYSANTHEMUM THIN CELL LAYERS (1)

Carbon source is an indispensable factor for the development of an in vitro morphogenic program of chrysanthemum micropropagation. The choice of carbon source affects the qualitative and quantitative outcome, and also the response of thin cell layers when these are placed onto morphogenic (callus, root, shoot, somatic embryo) media. Threshold survival levels (TSLs) could not be obtained for sucrose, fructose or glucose. TSLs together with organ differentiation were, however, obtained for mannose (60-80 g.L-1), xylose and lactose (40 g.L-1) and cellulose (60-80 g.L-1), making these carbon sources suitable as potential carbon sources for positive selection systems for chrysanthemum genetic transformation.
Circulating RANKL and RANKL/OPG and Breast Cancer Risk by ER and PR Subtype: Results from the EPIC Cohort

Receptor activator of nuclear factor-kappa B (RANK)-RANK ligand (RANKL) signaling promotes mammary tumor development in experimental models. Circulating concentrations of soluble RANKL (sRANKL) may influence breast cancer risk via activation of RANK signaling; this may be modulated by osteoprotegerin (OPG), the decoy receptor for RANKL. sRANKL and breast cancer risk by hormone receptor subtype has not previously been investigated. A case–control study was nested in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. This study included 1,976 incident invasive breast cancer cases [estrogen receptor positive (ER+), n = 1,598], matched 1:1 to controls. Women were pre- or postmenopausal at blood collection. Serum sRANKL was quantified using an ELISA, serum OPG using an electrochemiluminescent assay. Risk ratios (RR) and 95% confidence intervals (95% CI) were calculated using conditional logistic regression. Associations between sRANKL and breast cancer risk differed by tumor hormone receptor status (Phet = 0.05). Higher concentrations of sRANKL were positively associated with risk of ER+ breast cancer [5th vs. 1st quintile RR 1.28 (95% CI, 1.01–1.63); Ptrend = 0.20], but not ER− disease. For both ER+ and estrogen and progesterone receptor positive (ER+PR+) breast cancer, results considering the sRANKL/OPG ratio were similar to those for sRANKL; we observed a suggestive inverse association between the ratio and ER−PR− disease [5th vs. 1st quintile RR = 0.60 (0.31–1.14); Ptrend = 0.03]. This study provides the first large-scale prospective data on circulating sRANKL and breast cancer. We observed limited evidence for an association between sRANKL and breast cancer risk. Cancer Prev Res; 10(9); 525–34. ©2017 AACR.
The double adjuvants LTB and CpG significantly enhanced the immuno‐protective effects of recombinant GIT derived from Staphylococcus aureus and Streptococcus in mice

Purpose. In this study, we prepared GapC1‐150‐IsdB126‐361‐TRAP (GIT) proteins plus heat‐labile enterotoxin B (LTB) as an intra‐molecular adjuvant, together with CpG to further enhance its immunogenicity. Methodology. Initially, the target genes were acquired and inserted into pET‐32a (+) vectors to express LTB‐GIT protein. LTB‐GIT expression was confirmed by Western blotting and its immunocompetence was estimated through ELISA. Further, we immunized BALB/c mice with the LTB‐GIT plus CpG adjuvant. After the second immunization, the antigen‐specific CD4+ cell responses for IFN‐&ggr;, IL‐2, IL‐4 and IL‐10 were monitored by intracellular cytokine staining (ICS) assay. After the third immunization, the level of IgG antibodies in the serum from immunized groups was assessed by ELISA, and the protective immune response was appraised by Staphylococcus aureus and Streptococcus dysgalactiae challenge. Results. The ELISA results showed that the OD450nm value of the LTB‐GIT group was significantly higher than that of the BSA group. The group immunized with LTB‐GIT plus CpG exhibited significantly stronger CD4+ T cell responses for IFN‐&ggr;, IL‐2, IL‐4 and IL‐10 compared to the group immunized with LTB‐GIT, GIT alone orLTB‐GIT plus CpG. In addition, the group immunized with LTB‐GIT plus CpG generated the highest level of IgG antibodies against GIT among all of the groups, and our results also showed that LTB‐GIT plus CpG markedly improved the survival percentage of mice compared to other groups. Conclusion. We confirmed that the novel double adjuvants, LTB and CpG, are able to significantly improve GIT‐induced immune responses. This formula could be a promising strategy for enhancing the immune efficacy of multi‐subunit vaccines against Staphylococcus aureus and streptococcal infection.
Experimental Study of Effect of Polypropylene Fiber on Concrete Workability

Effect of different amount of fiber,different mixing process and tenuous admixture on dispersibility of polypropylene(PP) fiber was studied.At the same time,application of double admixture was used to compensate the drag effect of PP fiber on concrete fluidity thus the concrete has good plasticity and viscosity,its workability is much improved.The new ways and theoretical principle for mix proportion design of PP fiber reinforced concrete are provided by the indoor experimental study.