Miracle-dz/UniFAR-FTUs huggingface dataset:
Acanthamoeba castellanii : growth on human cell layers reactivates attenuated properties after prolonged axenic culture

The free-living, but potentially pathogenic, bacteriovorous amoebae of the genus Acanthamoeba can be easily grown axenically in a laboratory culture. This, however, often leads to considerable losses in virulence, and encystment capacity, and to changes in drug susceptibility. We evaluated potential options for a reactivation of a number of physiological properties, attenuated by prolonged axenic laboratory culture, including encystment potential, protease activity, heat resistance, growth rates and drug susceptibility against N-chlorotaurine (NCT). Toward this end, a strain that had been grown axenically for 10 years was repeatedly passaged on human HEp-2 cell monolayers or treated with 5′-azacytidine (AzaC), a methyltransferase inhibitor, and trichostatin A (TSA), a histone deacetylase inhibitor, in order to uplift epigenetic gene regulation. Culture on human cell monolayers resulted in significantly enhanced encystment potentials and protease activities, and higher susceptibility against NCT, whereas the resistance against heat shock was not altered. Treatment with AzaC/TSA resulted in increased encystment rates and protease activities, indicating the participation of epigenetic mechanisms. However, lowered resistances against heat shock indicate that possible stress responses to AzaC/TSA have to be taken into account. Repeated growth on human cell monolayers appears to be a potential method to reactivate attenuated characteristics in Acanthamoeba.
The effect of growth hormone replacement therapy on adrenal androgen secretion in adult onset hypopituitarism

objective Growth hormone replacement therapy in GH‐deficient children is associated with enhanced adrenal androgen production, raising the possibility that GH might stimulate adrenocortical hormone secretion. This has not been extensively investigated in adults to date. GH is a potent modulator of the activity of the 11β‐hydroxysteroid dehydrogenase type 1 (11β‐HSD1) enzyme and by altering cortisol metabolism can affect the function of the hypothalamo–pituitary–adrenal (HPA) axis and therefore potentially of adrenal androgen secretion. This study examined the effects of GH replacement in GH‐deficient adults on adrenal androgen secretion.
DETECTION AND GENETIC CHARACTERIZATION OF PORCINE CIRCOVIRUS 2 ISOLATES FROM THE FIRST CASES OF POSTWEANING MULTISYSTEMIC AND WASTING SYNDROME IN WILD BOARS IN GREECE

In 2002, postweaning multisystemic wasting syndrome (PMWS) was diagnosed in a European female wild boar (Sus scrofa), based on the detection of porcine circovirus 2 (PCV2) DNA in various organs, including the uterus, and on histopathologic lesions. This is the first detection of PCV2 DNA in the uterus of a wild boar. Three years later (2005), a wild boar <6–8 mo of age was found moribund. It presented wasting and dyspnea and finally died. PCV2 DNA was detected in tissue samples, and histopathologic lesions consistent with PMWS were observed. Both wild boars were from neighboring hunting areas in central Greece. Two PCV2 strains from the wild boars were genetically characterized and compared to other reported PCV2 sequences from wild boars and domestic pigs. The PCV-2 sequences from the wild boars in this study were closely related to each other and were grouped with two isolates from wild boars from Hungary. The phylogenetic analysis revealed that the virus might be transmitted between hunting areas. In addition, PCV2 may spread from domestic pigs to wild boars and vice versa.
Design by Contract for Python

The idea of design by contract (DEC), realized in the statically typed object-oriented programming language Eiffel, can be viewed as a systematic approach to specifying and implementing object-oriented software systems. We believe that a statically typed programming language is not suitable in the analysis and design phase of a prototyping-oriented software life cycle. For this purpose, dynamically typed interpreted programming languages are better suited. Unfortunately, dynamically typed programming languages usually do not support the concept of DEC. Therefore we integrated DEC into the programming language Python by using a metaprogramming approach, i.e., without changing the language or the run-time system. We adopted the DEC concept by adding mechanisms for dynamic type checking for method parameters and instance variables. The proposed combination of a more formal approach with a slim programming language provides a good basis for elicitation and documentation tasks in the analysis and design phase, especially in cases of a prototyping-oriented software development approach. Although the approach presented provides basic tool support for the analysis and design phase, further tool support especially for browsing assertions, is desirable.
Ethnic and gender differences in physical activity levels among 9-10-year-old children of white European, South Asian and African-Caribbean origin: the Child Heart Health Study in England (CHASE Study).

BACKGROUND Ethnic differences in physical activity in children in the UK have not been accurately assessed. We made objective measurements of physical activity in 9-10-year-old British children of South Asian, black African-Caribbean and white European origin. METHODS Cross-sectional study of urban primary school children (2006-07). Actigraph-GT1M activity monitors were worn by 2071 children during waking hours on at least 1 full day. Ethnic differences in mean daily activity [counts, counts per minute of registered time (CPM) and steps] were adjusted for age, gender, day of week and month. Multilevel modelling allowed for repeated days within individual and clustering within school. RESULTS In white Europeans, mean daily counts, CPM and mean daily steps were 394,785, 498 and 10,220, respectively. South Asian and black Caribbean children recorded more registered time per day than white Europeans (34 and 36 min, respectively). Compared with white Europeans, South Asians recorded 18 789 fewer counts [95% confidence interval (CI) 6390-31 187], 41 fewer CPM 95% CI 26-57) and 905 fewer steps (95% CI 624-1187). Black African-Caribbeans recorded 25 359 more counts (95% CI 14 273-36 445), and similar CPM, but fewer steps than white Europeans. Girls recorded less activity than boys in all ethnic groups, with 74 782 fewer counts (95% CI 66 665-82 899), 84 fewer CPM (95% CI 74-95) and 1484 fewer steps (95% CI 1301-1668). CONCLUSION British South Asian children have lower objectively measured physical activity levels than European whites and black African-Caribbeans.
Vanadyl sulfate lowers plasma insulin and blood pressure in spontaneously hypertensive rats.

Spontaneously hypertensive rats (SHR) are hyperinsulinemic compared with their Wistar-Kyoto (WKY) controls. Since previous studies have demonstrated that vanadyl sulfate lowers insulin levels in nondiabetic rats, we used vanadyl to explore the relation between hyperinsulinemia and hypertension. In a prevention study, 5-week-old SHR and WKY rats were started on long-term vanadyl sulfate treatment. Vanadyl in doses of 0.4 to 0.6 mmol/kg per day lowered plasma insulin (252 +/- 22.8 versus 336 +/- 12.6 pmol/L, treated versus untreated, P < .01) and systolic blood pressure (158 +/- 2 versus 189 +/- 1 mm Hg, P < .001) in SHR without causing any change in plasma glucose. No changes were seen in the treated WKY rats. At 11 weeks of age, a group of untreated rats from the prevention study was started on vanadyl treatment as before. Again, vanadyl caused significant and sustained decreases in plasma insulin (264 +/- 12.6 versus 342 +/- 6.6 pmol/L, treated versus untreated, P < .001) and blood pressure (161 +/- 1 versus 188 +/- 1 mm Hg, P < .001) in SHR but had no effect in the normotensive WKY controls. Furthermore, restoration of plasma insulin in the vanadyl-treated SHR to pretreatment levels (subcutaneous insulin, 14,000 pmol/kg per day) reversed the effects of vanadyl on blood pressure (vanadyl with insulin, 190 +/- 3.0 mm Hg versus vanadyl without insulin, 152 +/- 3.0 mm Hg, P < .001). Since vanadyl treatment resulted in decreased weight gain, treated SHR were compared with a corresponding pair-fed group. (ABSTRACT TRUNCATED AT 250 WORDS)
Microscale observables for heat and mass transport in sub-micron scale evaporating thin film

Microscale Observables for Heat and Mass Transport in Sub-Micron Scale Evaporating Thin Film. (May 2004) Sang-Kwon Wee, B.S., Pusan National University, Korea; M.S., Korea Advanced Institute of Science and Technology, Korea Chair of Advisory Committee: Dr. Kenneth D. Kihm A mathematical model is developed to describe the micro/nano-scale fluid flow and heat/mass transfer phenomena in an evaporating extended meniscus, focusing on the transition film region under nonisothermal interfacial conditions. The model incorporates thermocapillary stresses at the liquid-vapor interface, a slip boundary condition on the solid wall, polarity contributions to the working fluid field, and binary mixture evaporation. The analytical results show that the adsorbed film thickness and the thin film length decrease with increasing superheat by the thermocapillary stresses, which influences detrimentally the evaporation process by degrading the wettability of the evaporating liquid film. In contrast, the slip effect and the binary mixture enhance the stability of thin film evaporation. The slip effect at the wall makes the liquid in the transition region flow with smaller flow resistance and thus the length of the transition region increases. In addition, the total evaporative heat flow rate increases due to the slip boundary condition. The mixture of pentane and decane increases the length of the thin film by counteracting the thermocapillary stress, which enhances the stability of the thin film evaporation. The polarity effect of water significantly elongates the thin film length
Adenosine as an endogenous regulating factor of hippocampal sharp waves

The rodent hippocampus exhibits population activities called sharp waves (SPWs) during slow wave sleep and wake immobility. SPWs are important for hippocampal‐cortical communication and memory consolidation, and abnormal sharp wave‐ripple complexes are closely related to epileptic seizures. Although the SPWs are known to arise from the CA3 circuit, the local mechanisms underlying their generation are not fully understood. We hypothesize that endogenous adenosine is a local regulator of hippocampal SPWs. We tested this hypothesis in thick mouse hippocampal slices that encompass a relatively large hippocampal circuit and have a high propensity of generating spontaneous in vitro SPWs. We found that application of adenosine A1 receptor antagonists induced in vitro SPWs and that such induction was sensitive to blockade by NMDA receptor antagonists. By contrast, an increase in endogenous adenosine via pharmacological inhibition of adenosine transporters or adenosine degrading enzymes suppressed spontaneous in vitro SPWs. We thus suggest that the initiation and incidence of sharp wave‐like population events are under tight control by the activity of endogenously stimulated A1 receptors. © 2008 Wiley‐Liss, Inc.
Effectiveness of home respiratory polygraphy for the diagnosis of sleep apnoea and hypopnoea syndrome

Introduction Home respiratory polygraphy (HRP) may be a cost-effective alternative to polysomnography for the diagnosis of sleep apnoea-hypopnoea syndrome (SAHS), but stronger evidence is needed. Normally, patients transport HRP equipment from the hospital to home and back, which may create difficulties for some patients. Objectives To determine both the diagnostic efficacy and cost of HRP (with and without a transportation service moving the device and telematic transmission of data) in a large sample compared with in-hospital polysomnography. Methods Patients suspected of having SAHS were included in a multicentre study (eight hospitals). They were assigned to home and hospital protocols in random order. Receiver operating characteristic curves were constructed for manual respiratory polygraphy scoring protocol and different polysomnographic cut-off points. Diagnostic efficacies for several polysomnographic cut-off points were explored and costs for two equally effective alternatives were calculated. Results Of 366 randomised patients, 348 completed the protocol. The best receiver operating characteristic curve was obtained with a polysomnographic cut-off of the apnoea-hypopnoea index (AHI)≥5. The sensitive HRP AHI cut-off point (<5) had a sensitivity of 96%, a specificity of 57% and a negative likelihood ratio (LR) of 0.07; the specific cut-off (>10) had a sensitivity of 87%, a specificity of 86% and a positive LR of 6.25. The cost of HRP was half that of polysomnography. Telematic transmission costs were similar if the patients' costs were taken in to account. Conclusion HRP is an alternative to polysomnography in patients with suspected SAHS. Telematic procedures may help patients with limited mobility and those who live a long way from the sleep centre.
Apical electrogenic NaHCO3 cotransport. A mechanism for HCO3 absorption across the retinal pigment epithelium

Intracellular microelectrode techniques and intracellular pH (pHi) measurements using the fluorescent dye 2',7'-bis(carboxyethyl)-5(6)- carboxyfluorescein (BCECF) were employed to characterize an electrogenic bicarbonate transport mechanism at the apical membrane of the frog retinal pigment epithelium (RPE). Reductions in apical concentrations of both [HCO3]o (at constant Pco2 or pHo) or [Na]o caused rapid depolarization of the apical membrane potential (Vap). Both of these voltage responses were inhibited when the concentration of the other ion was reduced or when 1 mM diisothiocyano-2-2 disulfonic acid stilbene (DIDS) was present in the apical bath. Reductions in apical [HCO3]o or [Na]o also produced a rapid acidification of the cell interior that was inhibited by apical DIDS. Elevating pHi at constant Pco2 (and consequently [HCO3]i) by the addition of apical NH4 (20 mM) produced an immediate depolarization of Vap. This response was much smaller when either apical [HCO3]o or [Na]o was reduced or when DIDS was added apically. These results strongly suggest the presence of an electrogenic NaHCO3 cotransporter at the apical membrane. Apical DIDS rapidly depolarized Vap by 2-3 mV and decreased pHi (and [HCO3]i), indicating that the transporter moves NaHCO3 and net negative charge into the cell. The voltage dependence of the transporter was assessed by altering Vap with transepithelial current and then measuring the DIDS-induced change in Vap. Depolarization of Vap increased the magnitude of the DIDS-induced depolarization, whereas hyperpolarization decreased it. Hyperpolarizing Vap beyond -114 mV caused the DIDS- induced voltage change to reverse direction. Based on this reversal potential, we calculate that the stoichiometry of the transporter is 1.6-2.4 (HCO3/Na).
Schizotypal traits and psychotic-like experiences during adolescence: An update.

BACKGROUND The psychosis phenotype is distributed along a severity continuum that ranges from psychological well-being to full-blown psychosis. Schizotypal traits and psychotic-like experiences are considered as one of the possible phenotypic indicators of the latent liability for psychosis (named schizotypy). This selective review offers a general overview and update of trait schizotypy and psychotic like experiences during adolescence. METHOD The previous literature on schizotypal traits and psychotic like experiences is selectively reviewed. RESULTS We begin with a brief introduction of psychosis syndrome from an extended psychosis phenotype framework as well as a brief definition of trait schizotypy and psychotic-like experiences. We introduce the study of these traits and experiences from a developmental perspective, where the psychosis proneness-persistence-impairment model is highlighted. We provide a selective review of the tools available for assessment these constructs from the psychometric high-risk paradigm. We then discuss the factorial validity of the studies conducted in adolescents. In addition, the links between this set of subclinical traits and experiences and other variables gathered from a translational approach are discussed, with the aim to establish a nomological network. CONCLUSIONS We conclude by considering remaining questions and future directions for the understanding of trait schizotypy and psychotic-like experiences during adolescence.
Output signal-to-noise ratio and speech perception in noise: effects of algorithm

Abstract Objective: The aims of this study were to: 1) quantify the amount of change in signal-to-noise ratio (SNR) as a result of compression and noise reduction (NR) processing in devices from three hearing aid (HA) manufacturers and 2) use the SNR changes to predict changes in speech perception. We hypothesised that the SNR change would differ across processing type and manufacturer, and that improvements in SNR would relate to improvements in performance. Design: SNR at the output of the HAs was quantified using a phase-inversion technique. A linear mixed model was used to determine whether changes in SNR across HA conditions were predictive of changes in aided speech perception in noise. Study sample: Two groups participated: 25 participants had normal-hearing and 25 participants had mild to moderately severe sensorineural hearing loss. Results: The HAs programmed for both groups changed the SNR by a small, but statistically significant amount. Significant interactions in SNR changes were observed between HA devices and processing types. However, the change in SNR was not predictive of changes in speech perception. Conclusion: Although observed significant changes in SNR resulting from compression and NR did not convert to changes in speech perception, these algorithms may serve other purposes.
Brain CB2 Receptors: Implications for Neuropsychiatric Disorders

Although previously thought of as the peripheral cannabinoid receptor, it is now accepted that the CB2 receptor is expressed in the central nervous system on microglia, astrocytes and subpopulations of neurons. Expression of the CB2 receptor in the brain is significantly lower than that of the CB1 receptor. Conflicting findings have been reported on the neurological effects of pharmacological agents targeting the CB2 receptor under normal conditions. Under inflammatory conditions, CB2 receptor expression in the brain is enhanced and CB2 receptor agonists exhibit potent anti-inflammatory effects. These findings have prompted research into the CB2 receptor as a possible target for the treatment of neuroinflammatory and neurodegenerative disorders. Neuroinflammatory alterations are also associated with neuropsychiatric disorders and polymorphisms in the CB2 gene have been reported in depression, eating disorders and schizophrenia. This review will examine the evidence to date for a role of brain CB2 receptors in neuropsychiatric disorders.
Clinicians' attitudes to spirituality in old age psychiatry

Background: The aim of this survey is to investigate professional attitudes to the presence and value of spiritual care from Old Age Psychiatrists. Method: All registered members of the Faculty of the Psychiatry of Old Age in the United Kingdom were asked to complete a 21-question semi-structured questionnaire. The first mail shot took place in 2002 and the second mail shot to non-respondents in 2003. Quantitative and qualitative analyses were carried out on the answers received. Results: The response rate was 46%. The majority of respondents (92%) recognize the importance of spiritual dimensions of care for older people with mental health needs and about a quarter of respondents appear to consider referring patients to the chaplaincy service. In contrast, integration of spiritual advisors within the assessment and management of individual cases is rare. Conclusions: Opinions vary as to whether provision of spiritual care should become widely available to older people with mental health needs who are admitted to hospital. Old age psychiatrists recognize that awareness of spiritual dimensions may be important for their patients. They seem less clear about the role of spiritual advisors and how NHS multidisciplinary clinical teams and spiritual and pastoral care services can be best integrated. Much work needs to be done on developing effective training and operational policies in this area.
Analysing and evaluating usefulness and usability in electronic information services

Digital libraries, e-journal platforms, portals, e-prints and other web-based information systems provide services supporting users to perform intense work tasks that require complex interaction activities. The main components of such services are the users, the offered content and the system on which they are performed. This paper presents a model, which analyses the attributes of the electronic information services' components that affect user interaction and correlates them in the usefulness and usability evaluation process. An experimental study traces the relations between usefulness and usability, indicating that these evaluation parameters are interconnected and users do not find discriminating differences between them. The analysis of the content and system attributes suggests that user interaction is affected equally by content and system characteristics. Finally, the study illustrates users' preference for the attributes that constitute a useful system in contrast to those that support usability.
Detection of novel biomarkers of liver cirrhosis by proteomic analysis

Hepatic cirrhosis is a life‐threatening disease arising from different chronic liver disorders. One major cause for hepatic cirrhosis is chronic hepatitis C. Chronic hepatitis C is characterized by a highly variable clinical course, with at least 20% developing liver cirrhosis within 40 years. Only liver biopsy allows a reliable evaluation of the course of hepatitis C by grading inflammation and staging fibrosis, and thus serum biomarkers for hepatic fibrosis with high sensitivity and specificity are needed. To identify new candidate biomarkers for hepatic fibrosis, we performed a proteomic approach of microdissected cirrhotic septa and liver parenchyma cells. In cirrhotic septa, we detected an increasing expression of cell structure associated proteins, including actin, prolyl 4‐hydroxylase, tropomyosin, calponin, transgelin, and human microfibril–associated protein 4 (MFAP‐4). Tropomyosin, calponin, and transgelin reflect a contribution of activated stellate cells/myofibroblasts to chronic liver injury. The expression of tropomyosin, transgelin, and MFAP‐4, an extracellular matrix associated protein, were further evaluated by immunohistochemistry. Tropomyosin and MFAP‐4 demonstrated high serum levels in patients with hepatic cirrhosis of different causes. Conclusion: A quantitative analysis of MFAP‐4 serum levels in a large number of patients showed MFAP‐4 as novel candidate biomarker with high diagnostic accuracy for prediction of nondiseased liver versus cirrhosis [area under receiver operating characteristic curve (AUC) = 0.97, P < 0.0001] as well as stage 0 versus stage 4 fibrosis (AUC = 0.84, P < 0.0001), and stages 0 to 3 versus stage 4 fibrosis (AUC = 0.76, P < 0.0001). (HEPATOLOGY 2009.)