Miracle-dz/UniFAR-FTUs huggingface dataset:
Corneal Optical Regularization and Biomechanical Stabilization in Keratoconus and Irregular Astigmatism by Use of Topography-Guided Custom Ablation and Corneal Cross-Linking

Purpose: To evaluate one-step topography-guided transepithelial ablation in the treatment of low to moderate myopic astigmatism using a 1KHz excimer laser. Methods: Retrospective study of 117 consecutive eyes available for evaluation 12 months after surgery. Preand postoperative visual and refractive data as well as post-operative pain and haze were analyzed. A novel technique integrating custom refractiveand epithelialablation in a single uninterrupted procedure was used. Results: The mean pre-operative spherical equivalent (SE) and the mean cylinder were: –3.22 diopters (D) 61.54 (SD) (range –0.63 to –7.25 D) and –0.77 D 60.65 (range 0 to –4.50 D), respectively. At 12 months after surgery: no eyes lost $2 lines of corrected distant visual acuity (CDVA). Safety and efficacy indexes were 1.27 and 1.09, respectively. Uncorrected distant visual acuity (UDVA) was $20/20 in 96.6% of the eyes. Manifest refraction spherical equivalent was within 60.5 D of the desired refraction in 93.2% of the eyes. Average root mean square (RMS) wavefront error measured at central 6 mm, increased from 0.38 pre-operatively to 0.47 mm post-operatively. Refractive stability was achieved and sustained 1 month after surgery. No visually significant haze was registered during the observation period. Post-operative pain was reported in 4.5% of patients. Conclusions: One-step transepithelial topography-guided treatment for low to moderate myopia and astigmatism performed with a 1 KHz laser, provided safe, effective, predictable and stable results with low pain and no visually significant haze. Citation: Stojanovic A, Chen S, Chen X, Stojanovic F, Zhang J, et al. (2013) One-Step Transepithelial Topography-Guided Ablation in the Treatment of Myopic Astigmatism. PLoS ONE 8(6): e66618. doi:10.1371/journal.pone. 0066618 Editor: Andreas Wedrich, Medical University Graz, Austria Received December 27, 2012; Accepted May 8, 2013; Published June 17, 2013 Copyright: 2013 Stojanovic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by SynsLaser Kirurgi AS, Tromsø̧, Norway and Norges forskningsråd, Oslo, Norway. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: This study was funded by SynsLaser Kirurgi AS, Tromsø, together with Norges forskningsråd, Oslo, Norway. Authors Aleksandar Stojanovic, Xiangjun Chen, and Tor Paaske Utheim are employed by SynsLaser Kirurgi AS. This does not alter the authors’ adherence to all the PLOS ONE polices on sharing data and materials. * E-mail: aleks@online.no
Analysis and Control of M3C-Based UPQC for Power Quality Improvement in Medium/High-Voltage Power Grid

To enhance the power quality in the medium/high-voltage distribution power systems, a single-phase unified power quality conditioner (UPQC) based on the modular multilevel matrix converter (M3C) is presented in this paper. The M3C-UPQC is comprised of four identical multilevel converter arms and associated filtering inductors. According to the established equivalent circuit of M3C-UPQC, its operation principle and power balance of each arm are analyzed theoretically, and the parameters' design for the arm inductance as well as submodule capacitance is studied. Then, an integrated control method for M3C-UPQC in which the dc circulating current is used to balance the instantaneous active power of each arm is proposed to prevent the capacitor voltages from divergence inter- and intra-arms, so as to achieve voltages balance of M3C-UPQC. Finally, the effectiveness of the proposed control method is verified by a prototype rated at 8 kVA.
The Solution Structure of FADD Death Domain

A signal of Fas-mediated apoptosis is transferred through an adaptor protein Fas-associated death domain protein (FADD) by interactions between the death domains of Fas and FADD. To understand the signal transduction mechanism of Fas-mediated apoptosis, we solved the solution structure of a murine FADD death domain. It consists of six helices arranged in a similar fold to the other death domains. The interactions between the death domains of Fas and FADD analyzed by site-directed mutagenesis indicate that charged residues in helices α2 and α3 are involved in death domain interactions, and the interacting helices appear to interact in anti-parallel pattern, α2 of FADD with α3 of Fas and vice versa.
Determination of retinal cell fates is affected in the absence of extraocular striated muscles

Neural retinas of genetically modified mouse embryos and fetuses entirely lacking extraocular striated muscles (designated as Myf5‐/‐:MyoD‐/‐ or amyogenic) are used to study in vivo the role of extraocular muscle (i.e., fetal ocular movements) in the genesis of retinal cell diversity. Although retinal lamination and the total number of cells per retinal layer appeared unaffected in amyogenic fetuses, electron microscopy and histochemistry revealed the absence of cholinergic amacrine cell type. By contrast, the amounts of other amacrine cell subpopulations (calretinin‐, tyrosine hydroxylase‐, and parvalbumin‐expressing) were increased, whereas the amounts of Islet1/2‐expressing retinal ganglion cells were decreased. Surprisingly, it was not possible to detect any change in proliferation or cell death. Consistently, the number of progenitors for retinal ganglion cells (nestin‐expressing precursors) were increased, whereas the amounts of precursors for amacrine cells (syntaxin‐ and VC1.1‐expressing precursors) were decreased in the mutant retinas. The difference in requirements for extraocular muscle support in regulation of precise ratios of retinal neuronal cell types suggests an essential role of extrinsic cues in the determination of retinal cell fates. Taken together, it appears that patterning mechanisms intrinsic to the neural retina specify the basic organization of retinal spatial organization (e.g., retinal layers and total number of cells). However, extrinsic cues seem to change intrinsic properties (e.g., competence) of retinal progenitor cells and influence the ratios of the differentiated cell types (i.e., cell fate choice) they produce. Developmental Dynamics 226:000–000, 2003. © 2003 Wiley‐Liss, Inc.
Comparison of the Cancer Gene Targeting and Biochemical Selectivities of All Targeted Kinase Inhibitors Approved for Clinical Use

The anti-proliferative activities of all twenty-five targeted kinase inhibitor drugs that are in clinical use were measured in two large assay panels: (1) a panel of proliferation assays of forty-four human cancer cell lines from diverse tumour tissue origins; and (2) a panel of more than 300 kinase enzyme activity assays. This study provides a head-on comparison of all kinase inhibitor drugs in use (status Nov. 2013), and for six of these drugs, the first kinome profiling data in the public domain. Correlation of drug activities with cancer gene mutations revealed novel drug sensitivity markers, suggesting that cancers dependent on mutant CTNNB1 will respond to trametinib and other MEK inhibitors, and cancers dependent on SMAD4 to small molecule EGFR inhibitor drugs. Comparison of cellular targeting efficacies reveals the most targeted inhibitors for EGFR, ABL1 and BRAF(V600E)-driven cell growth, and demonstrates that the best targeted agents combine high biochemical potency with good selectivity. For ABL1 inhibitors, we computationally deduce optimized kinase profiles for use in a next generation of drugs. Our study shows the power of combining biochemical and cellular profiling data in the evaluation of kinase inhibitor drug action.
Continuous Time-Domain Analysis of Cerebrovascular Autoregulation Using Near-Infrared Spectroscopy

Background and Purpose— Assessment of autoregulation in the time domain is a promising monitoring method for actively optimizating cerebral perfusion pressure (CPP) in critically ill patients. The ability to detect loss of autoregulatory vasoreactivity to spontaneous fluctuations in CPP was tested with a new time-domain method that used near-infrared spectroscopic measurements of tissue oxyhemoglobin saturation in an infant animal model. Methods— Piglets were made progressively hypotensive over 4 to 5 hours by inflation of a balloon catheter in the inferior vena cava, and the breakpoint of autoregulation was determined using laser-Doppler flowmetry. The cerebral oximetry index (COx) was determined as a moving linear correlation coefficient between CPP and INVOS cerebral oximeter waveforms during 300-second periods. A laser-Doppler derived time-domain analysis of spontaneous autoregulation with the same parameters (LDx) was also determined. Results— An increase in the correlation coefficient between cerebral oximetry values and dynamic CPP fluctuations, indicative of a pressure-passive relationship, occurred when CPP was below the steady state autoregulatory breakpoint. This COx had 92% sensitivity (73% to 99%) and 63% specificity (48% to 76%) for detecting loss of autoregulation attributable to hypotension when COx was above a threshold of 0.36. The area under the receiver-operator characteristics curve for the COx was 0.89. COx correlated with LDx when values were sorted and averaged according to the CPP at which they were obtained (r=0.67). Conclusions— The COx is sensitive for loss of autoregulation attributable to hypotension and is a promising monitoring tool for determining optimal CPP for patients with acute brain injury.
Pattern minimization problems over recursive data types

In the context of program verification in an interactive theorem prover, we study the problem of transforming function definitions with ML-style (possibly overlapping) pattern matching into minimal sets of independent equations. Since independent equations are valid unconditionally, they are better suited for the equational proof style using induction and rewriting, which is often found in proofs in theorem provers or on paper. We relate the problem to the well-known minimization problem for propositional DNF formulas and show that it is £P/2-complete. We then develop a concrete algorithm to compute minimal patterns, which naturally generalizes the standard Quine-McCluskey procedure to the domain of term patterns.
Speculative query execution in Relational databases with Graph Modelling

In computer architecture, speculative execution is the process of executing instructions ahead of their normal schedule[1]. Grama et al.[2] introduce the concept of speculative decomposition as a possibility to execute one or more of possible branches in parallel with computation which are expected to determine the branch choice. The following paper introduces the method of speculative query execution in relational databases. Query queue can be seen as a line of sequential instructions and thus changing their order can result in some errors. Author introduce a middleware called the Speculative Layer which, based on a specific graph representation, executes some additional Speculative Queries. Results of those Speculative Queries can be used while executing queries from the queue providing a befit which is a shorter response time. The paper describes the process of graph modelling for groups of queries in order to initiate speculative computations, metrics used to evaluate Speculative Queries and experimental results for a test database and a group of input queries.
Testing Theories of Fairness - Intentions Matter

Recently developed models of fairness can explain a wide variety of seemingly contradictory facts. One of the most controversial and yet unresolved issues in the modeling of fairness preferences concerns the behavioral relevance of fairness intentions. Intuitively, fairness intentions seem to play an important role in economic relations, political struggles, and legal disputes but there is surprisingly little direct evidence for its behavioral importance. We provide experimental evidence for the behavioral relevance of fairness intentions in this paper. Our main result indicates that the attribution of fairness intentions is important in both the domains of negatively and positively reciprocal behavior. This means that equity models exclusively based on preferences over the distribution of material payoffs cannot capture reciprocal behavior. Models that take players' fairness intentions and distributional preferences into account are consistent with our data, while models that focus exclusively on intentions or on the distribution of material payoffs are not.
A Novel Lactobacilli-Based Teat Disinfectant for Improving Bacterial Communities in the Milks of Cow Teats with Subclinical Mastitis

Teat disinfection pre- and post-milking is important for the overall health and hygiene of dairy cows. The objective of this study was to evaluate the efficacy of a novel probiotic lactobacilli-based teat disinfectant based on changes in somatic cell count (SCC) and profiling of the bacterial community. A total of 69 raw milk samples were obtained from eleven Holstein-Friesian dairy cows over 12 days of teat dipping in China. Single molecule, real-time sequencing technology (SMRT) was employed to profile changes in the bacterial community during the cleaning protocol and to compare the efficacy of probiotic lactic acid bacteria (LAB) and commercial teat disinfectants. The SCC gradually decreased following the cleaning protocol and the SCC of the LAB group was slightly lower than that of the commercial disinfectant (CD) group. Our SMRT sequencing results indicate that raw milk from both the LAB and CD groups contained diverse microbial populations that changed over the course of the cleaning protocol. The relative abundances of some species were significantly changed during the cleaning process, which may explain the observed bacterial community differences. Collectively, these results suggest that the LAB disinfectant could reduce mastitis-associated bacteria and improve the microbial environment of the cow teat. It could be used as an alternative to chemical pre- and post-milking teat disinfectants to maintain healthy teats and udders. In addition, the Pacific Biosciences SMRT sequencing with the full-length 16S ribosomal RNA gene was shown to be a powerful tool for monitoring changes in the bacterial population during the cleaning protocol.
Cytochrome P450 1A2 Detoxicates Aristolochic Acid in the Mouse

Aristolochic acids (AAs) are plant-derived nephrotoxins and carcinogens responsible for chronic renal failure and associated urothelial cell cancers in several clinical syndromes known collectively as aristolochic acid nephropathy (AAN). Mice provide a useful model for study of AAN because the renal histopathology of AA-treated mice is strikingly similar to that of humans. AA is also a potent carcinogen in mice with a tissue spectrum somewhat different from that in humans. The toxic dose of AA in mice is higher than that in humans; this difference in susceptibility has been postulated to reflect differing rates of detoxication between the species. Recent studies in mice have shown that the hepatic cytochrome P450 system detoxicates AA, and inducers of the arylhydrocarbon response protect mice from the nephrotoxic effects of AA. The purpose of this study was to determine the role of specific cytochrome P450 (P450) enzymes in AA metabolism in vivo. Of 18 human P450 enzymes we surveyed only two, CYP1A1 and CYP1A2, which were effective in demethylating 8-methoxy-6-nitro-phenanthro-(3,4-d)-1,3-dioxolo-5-carboxylic acid (AAI) to the nontoxic derivative 8-hydroxy-6-nitro-phenanthro-(3,4-d)-1,3-dioxolo-5-carboxylic acid (AAIa). Kinetic analysis revealed similar efficiencies of formation of AAIa by human and rat CYP1A2. We also report here that CYP1A2-deficient mice display increased sensitivity to the nephrotoxic effects of AAI. Furthermore, Cyp1a2 knockout mice accumulate AAI-derived DNA adducts in the kidney at a higher rate than control mice. Differences in bioavailability or hepatic metabolism of AAI, expression of CYP1A2, or efficiency of a competing nitroreduction pathway in vivo may explain the apparent differences between human and rodent sensitivity to AAI.
Healthcare workers' hand decontamination practices: compliance with recommended guidelines.

AIM This paper reports a study of healthcare workers' handwashing/hand hygiene practices from a behavioural perspective. BACKGROUND Hospital acquired infection poses a very real and serious threat to all who are admitted to hospital. Pathogens are readily transmitted on healthcare workers' hands, and hand hygiene substantially reduces this transmission. Evidence-based guidelines for healthcare workers' hand hygiene practices exist, but compliance with these is internationally low. METHODS A quasi-experimental design with a convenient sample was used. The Predisposing, Reinforcing, Enabling Constructs in Educational Diagnosis and Evaluation Health Education Theory was used as the theoretical framework, and the data were collected in 2001. Healthcare workers' handwashing practices (observation of behaviour, n = 314) and their predisposition (attitudes, beliefs and knowledge) towards compliance with hand hygiene guidelines (questionnaire, n = 62) were studied. Nurses, doctors, physiotherapists and care assistants involved in direct patient care in the study unit participated in the study. The interventional hand hygiene programme aimed to predispose healthcare workers to adopt hand hygiene behaviour (poster campaign and educational handout), reinforce (feedback on pretest results) and enable the behaviour (provision of an alcohol hand rub beside each patients bedside). RESULTS Implementation of the multifaceted interventional behavioural hand hygiene programme resulted in an overall improvement in compliance with hand hygiene guidelines (51-83%, P < 0.001). Furthermore, healthcare workers believed that their skin condition improved (P < 0.001). An increase in knowledge about handwashing guidelines was also found. CONCLUSIONS In order to be effective, efforts to improve compliance with handwashing guidelines must be multifaceted. Alcohol hand rubs (with emollients) need to be provided at each patient's bedside. Issues surrounding healthcare workers' skin irritation need to be addressed urgently.
The Semantics of C++ Data Types: Towards Verifying low-level System Components

In order to formally reason about low-level system programs one needs a semantics (for the programming language in question) that can deal with programs that are not statically type-correct. For system-level programs, the semantics must deal with such heretical constructs like casting integers to pointers and converting pointers between incompatible base types. In this paper we describe a formal semantics for the data types of the C++ programming language that is suitable for low-level programs in the above sense. This work is part of a semantics for a large subset of the C++ programming language developed in the VFiasco project. In the VFiasco project we aim at the verification of substantial properties of the Fiasco microkernel, which is written in C++.
Electronic Cigarette Smoking Increases Arterial Stiffness and Oxidative Stress to a Lesser Extent Than a Single Conventional Cigarette: An Acute and Chronic Study.

The electronic cigarette (e-cig) has been proposed as a safer alternative than tobacco cigarettes (con-cigs)1,2 and as a bridge to smoking cessation. We aimed to examine its effects (1) on aortic stiffness as assessed by pulse wave velocity (PWV) and augmentation index (AIX75), (2) exhaled CO concentration, and (3) oxidative stress as assessed by malondialdehyde (MDA) plasma concentrations, both acutely and after 1 month of use. We studied 70 current smokers (age: 48±5 years, 56% female, 24±8 cigarettes/d, 30±7 pack-years, no other atherosclerotic risk factors or any medication) attending the hospital’s smoking cessation unit. Of those, 35 were assigned to use nicotine-free e-cig fluid (NOBACCO eGo Epsilon BDC 1100, eGo battery, 1100 mAh, operating at 3.9 V) during the acute-phase protocol, whereas the remaining 35 were assigned to an e-cig fluid with nicotine concentration of 12 mg/mL (propylene glycol 74.3%, glycerin 20%, flavoring 4.5%, nicotine 1.2%). All subjects underwent a baseline sham smoking for 7 minutes (inhaling on a nonlighted cigarette) and then were randomly assigned to smoke a single con-cig or vape an e-cig for 7 minutes (simulating the duration of a normal cigarette smoking). …
A Study of Workplace Violence Experienced by Doctors and Associated Risk Factors in a Tertiary Care Hospital of South Delhi, India.

INTRODUCTION The increasing incidences of violence against doctors in their workplaces are an important reason for stress among these healthcare workers. Many incidences of workplace violence against doctors have been reported in the past and are also being continuously reported from different parts of the country as well as the world. AIM To determine the prevalence of workplace violence among doctors and to study the associated risk factors in a tertiary care hospital of Delhi, India. MATERIAL AND METHODS A cross-sectional study was conducted using a self-administered semi-structured questionnaire. The contents were: data related to the workplace, incidences at work, violence prevention policy of the institution, reporting of incidences and follow-up, education and training for violence management. A total of 151 doctors participated in the study. RESULTS Total participants in the study were 151. The mean age of study participants was 26.73±4.24 years. Almost half (47.02%; 44.56% of males and 50.84% of females) of the doctors reported having an experience of violence during work hours in past 12 months. Among the cases, 39.4% were reported from Department of Obstetrics and Gynaecology while Surgery, Medicine and other departments reported 29.6%, 26.8% and 4.2% respectively. Patients or their relatives were perpetrators in most of the cases. Maximum (87.3%) of the reported cases were of verbal violence while 8.6% of the cases were of physical violence. Younger doctors with less work experience were more prone to physical violence. Regarding the time of violence, 35.1% of such cases occurred during afternoon while 30.1% of them took place at night. CONCLUSION A large number of doctors had experienced violence in past 12 months in a tertiary care hospital of South Delhi, India. Verbal violence came out to be most common form of violence experienced by the doctors. Afternoon or night hours were the timings when majority of such cases were reported.
Algal-lytic activities encoded by Chlorella virus CVK2.

Using a halo assay with E. coli lysates expressing Chlorella virus CVK2 genes on a cosmid contig, two different algal-lytic activities against Chlorella strain NC64A cells were found to be encoded on the CVK2 genome. The gene for vAL-1, one of the two activities, encoded a 349-aa ORF, which was homologous to PBCV-1 A215L and CVN1 CL-2. The vAL-1 gene was expressed at relatively early stages of the virus life cycle; transcripts and translation products appeared at 60 and 90 min postinfection, respectively. The vAL-1 protein was not incorporated into the viral particles but remained in the cell lysate, suggesting a role in the digestion of the cell wall before viral release at the final stage of infection. Cell wall materials isolated from Chlorella strain NC64A cells were digested by vAL-1 and degradation products were detected on TLC. In addition to Chlorella strain NC64A, vAL-1 lysed cells of four C. vulgaris strains as well as Chlorella sp. SAG-241-80.