Miracle-dz/UniFAR-FTUs huggingface dataset:
Arteriosclerosis, Thrombosis, and Vascular Biology

BACKGROUND: BETs (bromodomain and extraterminal domain–containing epigenetic reader proteins), including BRD4 (bromodomain-containing protein 4), orchestrate transcriptional programs induced by pathogenic stimuli, as intensively studied in cardiovascular disease and elsewhere. In endothelial cells (ECs), BRD4 directs induced proinflammatory, proatherosclerotic transcriptional responses; BET inhibitors, like JQ1, repress these effects and decrease atherosclerosis. While BET effects in pathogenic conditions have prompted therapeutic BET inhibitor development, BET action under basal conditions, including ECs, has remained understudied. To understand BET action in basal endothelial transcriptional programs, we first analyzed EC RNA-Seq data in the absence versus presence of JQ1 before using BET regulation to identify novel determinants of EC biology and function. METHODS: RNA-Seq datasets of human umbilical vein ECs without and with JQ1 treatment were analyzed. After identifying C12orf34, also known as FAM222A (family with sequence similarity 222 member A), as a previously unreported, basally expressed, potently JQ1-induced EC gene, FAM222A was studied in endothelial and angiogenic responses in vitro using small-interference RNA silencing and lentiviral overexpression, in vitro, ex vivo and in vivo, including aortic sprouting, matrigel plug assays, and murine neonatal oxygen-induced retinopathy. RESULTS: Resting EC RNA-Seq data indicate BETs direct transcriptional programs underlying core endothelial properties including migration, proliferation, and angiogenesis. BET inhibition in resting ECs also significantly induced a subset of mRNAs, including FAM222A—a unique BRD4-regulated gene with no reported EC role. Silencing endothelial FAM222A significantly decreased cellular proliferation, migration, network formation, aorta sprouting, and Matrigel plug vascularization through coordinated modulation of VEGF (vascular endothelial growth factor) and NOTCH mediator expression in vitro, ex vivo, in vivo; lentiviral FAM222A overexpression had opposite effects. In vivo, siFAM222A significantly repressed retinal revascularization in neonatal murine oxygen-induced retinopathy through similar angiogenic signaling modulation. CONCLUSIONS: BET control over the basal endothelial transcriptome includes FAM222A, a novel, BRD4-regulated, key determinant of endothelial biology and angiogenesis.
Phonological Treatment Approaches for Spoken Word Production in Aphasia

Abstract This article provides an overview of phonological treatment approaches for anomia in individuals with aphasia. The role of phonology in language processing, as well as the impact of phonological impairment on communication is initially discussed. Then, traditional phonologically based treatment approaches, including phonological, orthographic, indirect, guided, and mixed cueing methods, are described. Collectively, these cueing treatment approaches aim to facilitate word retrieval by stimulating residual phonological abilities. An alternative treatment approach, phonomotor treatment, is also examined. Phonomotor treatment aims to rebuild sublexical, phonological sequence knowledge and phonological awareness as a means to strengthen lexical processing and whole-word naming. This treatment is supported by a parallel-distributed processing model of phonology and therefore promotes multimodal training of individual phonemes and phoneme sequences in an effort to enhance the neural connectivity supporting underlying phonological processing mechanisms. The article concludes with suggestions for clinical application and implementation.
Approximation-Based Adaptive Tracking Control for MIMO Nonlinear Systems With Input Saturation

In this paper, an approximation-based adaptive tracking control approach is proposed for a class of multiinput multioutput nonlinear systems. Based on the method of neural network, a novel adaptive controller is designed via backstepping design process. Furthermore, by introducing Nussbaum function, the issue of unknown control directions is handled. In the backstepping design process, the dynamic surface control technique is employed to avoid differentiating certain nonlinear functions repeatedly. Moreover, in order to reduce the number of adaptation laws, we do not use the neural networks to directly approximate the unknown nonlinear functions but the desired control signals. Finally, we provide two examples to illustrate the effectiveness of the proposed approach.
Chromosomal aberrations and schizophrenia

Chromosomal aberrations associated with schizophrenic disorders may suggest regions in which to focus a search for genes predisposing to schizophrenia by a linkage strategy. As for other genetic illnesses, chromosomal abnormalities may also provide useful tools for subsequent physical mapping, fine localisation, and isolation of important susceptibility genes. Identification of several chromosomal aberrations may be especially important, given the unknown pathophysiology, the paucity of known brain genes, and the probable genetic heterogeneity of schizophrenia and manic-depression. However, because psychiatric disorders are common and inherited in a complex manner, researchers must use caution when drawing inferences about associations with chromosomal aberrations. Reported abnormalities involving autosomes (chromosomes 1–22) associated with psychotic disorders are reviewed. Their relevance to linkage studies localising genes for schizophrenia was estimated by standardised criteria for specificity, diagnosis, family history, and overall weight of evidence. Four ‘possibly relevant’ chromosomal regions were identified: 5q, 11q, 18q, and 19p. This paper outlines strategies for future studies to detect new chromosomal aberrations associated with major psychotic disorders that may be relevant to isolating the genes for schizophrenia.
QUANTIZATION OF MAGNITUDE SPECTRA IN WAVEFORM INTERPOLATION SPEECH CODING

Efficient quantization of magnitude spectra is one of the central issues in speech coding, especially with techniques operating at low bit rates. This paper presents a novel high-accuracy quantization scheme for the magnitude spectra in waveform interpolation speech coding. The performance of the proposed techniques, including both their accuracy and complexity, is studied using objective measures. In addition, the techniques are compared to conventional approaches. On the basis of these comparisons, it can be concluded that the techniques presented in this paper are capable of achieving high quantization accuracy. Thus, the proposed techniques can be employed in waveform interpolation speech coders to enhance the quality of the reconstructed speech.
The Prevalence of Polycystic Ovaries on Ultrasound Scanning in a Population of Randomly Selected Women

Summary: Polycystic ovaries (PCO) diagnosed by ultrasound have been commonly reported amongst healthy women. The study aimed to determine the prevalence of PCO in a population of women from the community, and to relate it to clinical and endocrinological data. Twelve hundred women chosen randomly from electoral rolls were invited to take part in the study. Two hundred and fifty five women (21%) who met eligibility criteria agreed to participate and 183 women (16%) finally completed the study. Seventy two women did not attend. An ultrasound scan and blood tests were taken on day 5–9 of their menstrual cycles. Data about their menstrual periods and reproductive history was collected. The prevalence of PCO was 21% (39 of 183). No differences existed between women with PCO and normal ovaries with respect to uterine size, body mass index, luteinizing hormone levels, serum hormone binding globulin levels and fertility status. Hirsutism (Ferriman Gallwey score >7), elevated testosterone levels and irregular menstrual cycles were significantly more frequent amongst women with PCO. Mean ovarian volume was larger in women with PCO irrespective of the use of hormonal contraception. Fifty nine per cent of women with PCO had irregular menstrual cycles or elevated Ferriman Gallwey scores or both. There was no detectable effect of PCO on parity or miscarriage although only 70% of women with PCO had evidence of an adequate ovulation compared to 95% of women with normal ovaries.
Intrapopulation Diet Variation in Four Frogs (Leptodactylidae) of the Brazilian Savannah

Abstract Age and sex-based as well as individual-level diet variation are known to occur in many natural populations, and may have important ecological and evolutionary implications. In the case of individual-level diet variation, most examples come from species-poor, temperate communities, and it is currently believed that it results from population niche expansion following interspecific competitive release. We investigated and measured the intrapopulation diet variation in four species of frogs, Leptodactylus ( = Adenomera) sp. , Eleutherodactylus cf. juipoca, L. fuscus, and Proceratophrys sp. , that are part of species-rich frog communities of the Brazilian Cerrado. Specifically, we investigated age and sex-related, as well as individual-level, diet variation. We measured individual-level diet variation with the IS index of individual specialization, which is a measure of the degree of overlap between individual niches and the population niches. We found no ontogenetic shifts or sex-related differences in the types of prey consumed. However, we found evidence of individual-level diet variation in the four studied species (IS ˜ 0.2–0.5). There was a negative correlation between IS and the population niche width (r = –0.980; P < 0.0001), indicating that interindividual diet variation is more pronounced in more generalized populations. This pattern suggests that individual niche widths remain constrained even when population niche breadth is wide, consistent with the presence of functional trade-offs. We found no evidence that these trade-offs arise from morphology, since there was no diet–morphology correlation. We hypothesize that trade-offs have a behavioral or physiological basis, which needs further investigation. This is the first documented case of individual-level diet variation in a diverse tropical community, indicating that this phenomenon is not restricted to competitive release-driven niche expansion in temperate, depauperate communities.
Integration along the Abuja road map - A progress report

This paper reviews integration among the eight African Regional Economic Communities by comparing their characteristics and progress with three other South–South Regional Integration Arrangements. Three conclusions emerge: (i) slow progress towards meeting overly ambitious objectives; (ii) small changes in the destination of trade across all Regional Economic Communities, indicative of persistent high trade costs and few new manufactures products destined for geographically close markets; and (iii) compared with other South–South Regional Integration Arrangements, the Regional Economic Communities include a high number of provisions not covered in Word Trade Organization negotiations, but these have low legal enforceability. Reasons for this slow progress are explored in the paper.
Value Maximization, Stakeholder Theory, and the Corporate Objective Function*

Abstract: In this article, I offer a proposal to clarify what I believe is the proper relation between value maximization and stakeholder theory, which I call enlightened value maximization. Enlightened value maximization utilizes much of the structure of stakeholder theory but accepts maximization of the long-run value of the firm as the criterion for making the requisite tradeoffs among its stakeholders, and specifies long-term value maximization or value seeking as the firm’s objective. This proposal therefore solves the problems that arise from the multiple objectives that accompany traditional stakeholder theory. I also discuss the Balanced Scorecard, which is the managerial equivalent of stakeholder theory, explaining how this theory is flawed because it presents managers with a scorecard that gives no score—that is, no single-valued measure of how they have performed. Thus managers evaluated with such a system (which can easily have two dozen measures and provides no information on the tradeoffs between them) have no way to make principled or purposeful decisions. The solution is to define a true (single dimensional) score for measuring performance for the organization or division (and it must be consistent with the organization’s strategy), and as long as their score is defined properly, (and for lower levels in the organization it will generally not be value) this will enhance their contribution to the firm.
Using Data-driven and Phonetic Units for Speaker Verification

Recognition of speaker identity based on modeling the streams produced by phonetic decoders (phonetic speaker recognition) has gained popularity during the past few years. Two of the major problems that arise when phone based systems are being developed are the possible mismatches between the development and evaluation data and the lack of transcribed databases. Data-driven segmentation techniques provide a potential solution to these problems because they do not use transcribed data and can easily be applied on development data minimizing the mismatches. In this paper we compare speaker recognition results using phonetic and data-driven decoders. To this end, we have compared the results obtained with a speaker recognition system based on data-driven acoustic units and phonetic speaker recognition systems trained on Spanish and English data. Results obtained on the NIST 2005 Speaker Recognition Evaluation data show that the data-driven approach outperforms the phonetic one and that further improvements can be achieved by combining both approaches
Altered Cytochrome P450 Expression in Mice during Pregnancy

Human pregnancy is known to influence hepatic drug metabolism in a cytochrome (P450)-specific manner. However, the underlying mechanisms remain unknown, in part due to a lack of experimental models to study altered drug metabolism during pregnancy. In this study, we examined how pregnancy influences expression of major P450 isoforms in mice. Liver tissues were isolated from female FVB/N-mice at different gestational time points: prepregnancy, 7, 14, and 21 days of pregnancy, and 7 days postpartum. mRNA expression levels of major P450 isoforms (Cyp1a2, Cyp2a5, Cyp2b10, Cyp2c37, Cyp2d22, Cyp2e1, Cyp3a11, and Cyp3a41) in the liver tissues were determined by quantitative real-time polymerase chain reaction. Whereas Cyp2a5 expression was unchanged, Cyp3a41 expression was significantly increased during pregnancy. In contrast, expression of Cyp1a2, Cyp2c37, Cyp2d22, Cyp2e1, and Cyp3a11 was decreased. Expression of Cyp2d22 and Cyp2e1 isoforms correlated with that of peroxisome proliferator-activated receptor (PPAR)α in the mouse livers, suggesting potential involvement of PPARα in down-regulation of the P450 expression during pregnancy. Effects of pregnancy on expression of other P450 mouse isoforms as well as on in vivo drug disposition remain to be characterized. These results provide a guide for future studies on P450 regulation during pregnancy.
Knowledge, Attitudes, and Practice Related to Cervical Cancer Screening among Kuwaiti Women

Objectives: To assess the knowledge, attitude, and practice regarding cervical cancer screening among Kuwaiti women. Subjects and Methods: A total of 300 married Kuwaiti women were randomly selected from those who visited the clinics irrespective of reason(s) for the visit. A structured questionnaire covering sociodemographic characteristics, knowledge, attitude, and practice related to cervical cancer screening was administered. Results: Of the 300 women, complete information was collected from 281 (93.7%), the knowledge about the test was adequate in 147 (52.3%) women, while 86 (30.6%) had adequate attitude towards the test and only 67 (23.8%) had an adequate practice. The main reason given for not having had a Papanicolaou smear was that it was not suggested by the doctor. Among all the respondents 220 (78.7%) would prefer a female doctor to conduct the test. The level of education was the only significant factor independently associated with inadequate knowledge and attitude towards Papanicolaou smear test (p = 0.006 and p = 0.001, respectively) when adjusted for the effect of other factors in multivariate logistic regression analysis. However, age (p < 0.001), level of education (p = 0.028), and number of years since last visit with a gynaecologist (p = 0.005) were significant factors independently associated with inadequate practice of the test. Conclusion: Our findings showed that a well-designed health education programme on cervical cancer and benefits of screening would increase the awareness among Kuwaiti women.
Targeting of secretory vesicles to cytoplasmic domains in AtT-20 and PC- 12 cells

Organelles are not uniformly distributed throughout the cytoplasm but have preferred locations that vary between tissues and during development. To investigate organelle targeting to cytoplasmic domains we have taken advantage of the mouse pituitary cell line, AtT-20, which, when induced to extend long processes, accumulates dense core secretory granules at the tips of the processes. During mitosis, these secretory granules accumulate along the plane of division. Protein synthesis is not mandatory for such redistribution of secretory granules. To explore the specificity of the redistribution we have used transfected AtT-20 cells that express the immunoglobulin kappa light chain. While the endogenous hormone ACTH is found in secretory granules, the kappa chain is a marker for organelles involved in constitutive secretion. By immunofluorescence, kappa also accumulates at the tips of growing processes, and along the midline of dividing cells, suggesting that the redistribution of vesicles is not specific for dense-core secretory granules. Since there is evidence for selective organelle transport along processes in neuronal cells, the rat pheochromocytoma cell PC-12 was transfected with DNA encoding markers for regulated and constitutive secretory vesicles. Again regulated and constitutive vesicles co-distribute, even in cells grown in the presence of nerve growth factor. We suggest that at least in the cells studied here, cytoskeletal elements normally carry exocytotic organelles to the surface; when the cytoskeletal elements coalesce in an extending process, exocytotic organelles of both the constitutive and regulated pathway are transported nonselectively to the tips of the cytoskeletal elements where they accumulate.
The Role of the Southern Ocean in Abrupt Transitions and Hysteresis in Glacial Ocean Circulation

High‐latitude Northern Hemisphere climate during the last glacial period was characterized by a series of abrupt climate changes, known as Dansgaard‐Oeschger events, which were recorded in Greenland ice cores as shifts in the oxygen isotopic composition of the ice. These shifts in inferred Northern Hemisphere high‐latitude temperature have been linked to changes in Atlantic meridional overturning strength. The response of ocean overturning circulation to forcing is nonlinear and a hierarchy of models have suggested that it may exist in multiple steady state configurations. Here, we use a time‐dependent coarse‐resolution isopycnal model with four density classes and two basins, linked by a Southern Ocean to explore overturning states and their stability to changes in external parameters. The model exhibits hysteresis in both the steady state stratification and overturning strength as a function of the magnitude of North Atlantic Deep Water formation. Hysteresis occurs as a result of two nonlinearities in the model—the surface buoyancy distribution in the Southern Ocean and the vertical diffusivity profile in the Atlantic and Indo‐Pacific basins. We construct a metric to assess circulation configuration in the model, motivated by observations from the Last Glacial Maximum, which show a different circulation structure from the modern. We find that circulation configuration is primarily determined by North Atlantic Deep Water density. The model results are used to suggest how ocean conditions may have influenced the pattern of Dansgaard‐Oeschger events across the last glacial cycle.
Proteomic profiling of pancreatic ductal carcinoma cell lines treated with trichostatin‐A

A pancreatic adenocarcinoma cell line (Paca44) was treated with trichostatin‐A (TSA), a potent inhibitor of histone deacetylases, in order to evaluate the effect of this drug on protein expression. Master maps of control and treated Paca44 cells were generated by analysis with the PDQuest software. The comparison between such maps showed up‐ and downregulation of 51 polypeptide chains, out of a total of 700 spots detected by a medium‐sensitivity stain, micellar Coomassie Brilliant Blue. Fingerprinting by matrix‐assisted laser desorption/ionization‐time of flight (MALDI‐TOF)‐mass spectrometry analysis enabled the identification of 22 of these spots. Among these proteins, of particular interest are the two downregulated proteins nucleophosmin and translationally controlled tumor protein, as well as the upregulated proteins programmed cell death protein 5 (also designated as TFAR19) and stathmin (oncoprotein 18). The modulation of these four proteins is consistent with our observation that TSA is able to inhibit cell growth of Paca44 by causing cell cycle arrest at the G2 phase and apoptotic cell death.
From transportation patterns to power demand: Stochastic modeling of uncontrolled domestic charging of electric vehicles

This paper presents a Monte Carlo simulation approach for the modeling of the power demand of electric vehicles under the scenario of uncontrolled domestic charging. A detailed transportation dataset for the Netherlands is used to derive the stochastic characteristics of the behavior of vehicles. The stochastic variables are the start/end-time of each trip and the respective travelled distance while the battery state of charge at the beginning of charging is derived by the consideration of the distance traveled since the last charging and the charging history. The stochastic variables are modeled using normal copula function based on the respective correlations and marginal distributions. The total load due to electric vehicles is computed based on the combination of the simulated commuting pattern with the charging profile of a typical electric vehicle battery. The results show that the EV power demand reaches the highest value during the evening peak hours for the residential load, however the peak is significantly lower than maximum which is mainly caused by the low charging time due to a generally low mean traveled distance.