Miracle-dz/UniFAR-FTUs huggingface dataset:
Open biological reduction and a locking compression plate for distal femoral fractures: a review of 40 cases.

OBJECTIVE To determine the clinical results of patients with comminuted fractures of the distal femur treated by open biological reduction using an indirect reduction technique and locking compression plate (LCP) without primary bone grafting. MATERIAL AND METHOD Prospective observation was conducted with 40 patients (24 males and 16 females), average age 48.7 years (range 20-81), with distal femoral fractures AO/OTA types A2 (5), A3 (17), C2 (8) and C3 (10) who were treated using an open indirect reduction technique and fixation with LCP between May 2010 and December 2013. Among the 40 patients, 28 were closed fractures and 12 were open fractures. Clinical results were evaluated using the Neer score six months after surgery. Follow-up periods ranged from 12 to 36 months (average 18.35). RESULTS Thirty eight (95%) of the fractures healed completely without a secondary procedure. The average time to union was 14.45 weeks (range 12-24). The average knee range of motion was 2° (0-5) to 110° (20-140). Two patients had an implant failure which required revision and secondary iliac bone grafting. There were no varus or valgus deformities, no limb shortening and no deep infections. Neer scores were excellent in 18 cases (45%), good in 14 (35%), fair in 6 (15%) and poor in 2 (5%). The mean Neer score was 83.60 (range 50-100). CONCLUSION The indirect reduction technique for the treatment of comminuted distal femoral fractures provides satisfactory results when combined with LCP fixation.
Sall4 controls differentiation of pluripotent cells independently of the Nucleosome Remodelling and Deacetylation (NuRD) complex

Sall4 is an essential transcription factor for early mammalian development and is frequently overexpressed in cancer. Although it is reported to play an important role in embryonic stem cell (ESC) self-renewal, whether it is an essential pluripotency factor has been disputed. Here, we show that Sall4 is dispensable for mouse ESC pluripotency. Sall4 is an enhancer-binding protein that prevents precocious activation of the neural gene expression programme in ESCs but is not required for maintenance of the pluripotency gene regulatory network. Although a proportion of Sall4 protein physically associates with the Nucleosome Remodelling and Deacetylase (NuRD) complex, Sall4 neither recruits NuRD to chromatin nor influences transcription via NuRD; rather, free Sall4 protein regulates transcription independently of NuRD. We propose a model whereby enhancer binding by Sall4 and other pluripotency-associated transcription factors is responsible for maintaining the balance between transcriptional programmes in pluripotent cells. Highlighted article: Sall4 and Sall1 inhibit neural differentiation in ESCs by acting at enhancer sequences independently of the NuRD complex, and are dispensable for the maintenance of pluripotency.
Analytical upstream collocation solution of a quadratically forced steady‐state convection‐diffusion equation

Purpose – The purpose of this paper is to present the analytical solution to the Hermite collocation discretization of a quadratically forced steady‐state convection‐diffusion equation in one spatial dimension with constant coefficients, defined on a uniform mesh, with Dirichlet boundary conditions. To improve the accuracy of the method “upstream weighting” of the convective term is used in an optimal way. The authors also provide a method to determine where the forcing function should be optimally sampled. Computational examples are given, which support and illustrate the theory of the optimal sampling of the convective and forcing term.Design/methodology/approach – The authors: extend previously published results (which dealt only with the case of linear forcing) to the case of quadratic forcing; prove the theorem that governs the quadratic case; and then illustrate the results of the theorem using computational examples.Findings – The algorithm developed for the quadratic case dramatically decreases th.
Curves of Genus 2, Continued Fractions, and Somos Sequences

We detail the continued fraction expansion of the square root of monic sextic polynomials. We note in passing that each line of the expansion corresponds to addition of the divisor at inflnity, and interpret the data yielded by the general expansion. In particular we obtain an associated Somos sequence deflned by a three-term recurrence relation of width 6.
Ultrastructural differences between diabetic and idiopathic gastroparesis

The ultrastructural changes in diabetic and idiopathic gastroparesis are not well studied and it is not known whether there are different defects in the two disorders. As part of the Gastroparesis Clinical Research Consortium, full thickness gastric body biopsies from 20 diabetic and 20 idiopathic gastroparetics were studied by light microscopy. Abnormalities were found in many (83%) but not all patients. Among the common defects were loss of interstitial cells of Cajal (ICC) and neural abnormalities. No distinguishing features were seen between diabetic and idiopathic gastroparesis. Our aim was to provide a detailed description of the ultrastructural abnormalities, compare findings between diabetic and idiopathic gastroparesis and determine if patients with apparently normal immunohistological features have ultrastructural abnormalities. Tissues from 40 gastroparetic patients and 24 age‐ and sex‐matched controls were examined by transmission electron microscopy (TEM). Interstitial cells of Cajal showing changes suggestive of injury, large and empty nerve endings, presence of lipofuscin and lamellar bodies in the smooth muscle cells were found in all patients. However, the ultrastructural changes in ICC and nerves differed between diabetic and idiopathic gastroparesis and were more severe in idiopathic gastroparesis. A thickened basal lamina around smooth muscle cells and nerves was characteristic of diabetic gastroparesis whereas idiopathic gastroparetics had fibrosis, especially around the nerves. In conclusion, in all the patients TEM showed abnormalities in ICC, nerves and smooth muscle consistent with the delay in gastric emptying. The significant differences found between diabetic and idiopathic gastroparesis offers insight into pathophysiology as well as into potential targeted therapies.
Doctors' emotional reactions to recent death of a patient: cross sectional study of hospital doctors

Abstract Objectives: To describe doctors' emotional reactions to the recent death of an “average” patient and to explore the effects of level of training on doctors' reactions. Design: Cross sectional study using quantitative and qualitative data. Setting: Two academic teaching hospitals in the United States. Participants: 188 doctors (attending physicians (equivalent to UK consultants), residents (equivalent to UK senior house officers), and interns (equivalent to UK junior house officers)) who cared for 68 patients who died in the hospital. Main outcome measures: Doctors' experiences in providing care, their emotional reactions to the patient's death, and their use of coping and social resources to manage their emotions. Results: Most doctors (139/188, 74%) reported satisfying experiences in caring for a dying patient. Doctors reported moderate levels of emotional impact (mean 4.7 (SD 2.4) on a 0-10 scale) from the death. Women and those doctors who had cared for the patient for a longer time experienced stronger emotional reactions. Level of training was not related to emotional reactions, but interns reported needing significantly more emotional support than attending physicians. Although most junior doctors discussed the patient's death with an attending physician, less than a quarter of interns and residents found senior teaching staff (attending physicians) to be the most helpful source of support. Conclusions: Doctors who spend a longer time caring for their patients get to know them better but this also makes them more vulnerable to feelings of loss when these patients die. Medical teams may benefit from debriefing within the department to give junior doctors an opportunity to share emotional responses and reflect on the patient's death.
7,8-Dihydroxyflavone, a Tropomyosin-Kinase Related Receptor B Agonist, Produces Fast-Onset Antidepressant-Like Effects in Rats Exposed to Chronic Mild Stress

Objective Brain-derived neurotrophic factor (BDNF) and its specific receptor, tropomyosin-related kinase (TrkB), play important roles in treating depression. In this experiment, we examined whether 7,8-dihydroxyflavone, a novel potent TrkB agonist, could reverse the behavioral and biochemical abnormalities induced by the chronic mild stress (CMS) paradigm in rats. Methods SD rats were exposed to a battery of stressors for 56 days. 7,8-dihydroxyflavone (5 and 20 mg/kg) were administered intraperitoneally during the last 28 days of the CMS paradigm. Rats were tested in sucrose consumption test (SCT), forced-swimming test (FST) and elevated T-maze (ETM). Serum corticosterone levels and hippocampal BDNF levels of the rats were measured. Results Four-week CMS on the rats induced their depression-like behavior in SCT. The CMS-reduced sucrose consumption was reversed starting from 7 days after the 7,8-dihydroxyflavone (20 mg/kg) treatment and remained across the subsequent treatment regime. 7,8-dihydroxyflavone, when given at 5 mg/kg for 3 weeks, reduced the immobility time in the FST in the CMS-subjected rats. Additionally, the 4-week treatment with 7,8-dihydroxyflavone (20 mg/kg) attenuated the CMS-induced increase in anxiety-like behavior in the ETM. For the CMS-subjected rats, 7,8-dihydroxyflavone treatment dose-dependently reduced their serum corticosterone levels but increased their hippocampal BDNF levels only at 5 mg/kg. Conclusion 7,8-dihydroxyflavone was beneficial for both depression and anxiety-like behaviors, and may exert fast-onset antidepressant effects. This provides a new insight into the pharmacological management of depression.
Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration

New optogenetic tools are introduced that provide spatial and temporal control over the activity of heterotrimeric G protein subunits inside single cells. They are used to identify dynamic roles for G protein subunits in immune cell migration. They can be applied to study the mechanistic basis of other GPCR-regulated cellular functions.
PI(4,5)P2-dependent microdomain assemblies capture microtubules to promote and control leading edge motility

The lipid second messenger PI(4,5)P2 modulates actin dynamics, and its local accumulation at plasmalemmal microdomains (rafts) might mediate regulation of protrusive motility. However, how PI(4,5)P2-rich rafts regulate surface motility is not well understood. Here, we show that upon signals promoting cell surface motility, PI(4,5)P2 directs the assembly of dynamic raft-rich plasmalemmal patches, which promote and sustain protrusive motility. The accumulation of PI(4,5)P2 at rafts, together with Cdc42, promotes patch assembly through N-WASP. The patches exhibit locally regulated PI(4,5)P2 turnover and reduced diffusion-mediated exchange with their environment. Patches capture microtubules (MTs) through patch IQGAP1, to stabilize MTs at the leading edge. Captured MTs in turn deliver PKA to patches to promote patch clustering through further PI(4,5)P2 accumulation in response to cAMP. Patch clustering restricts, spatially confines, and polarizes protrusive motility. Thus, PI(4,5)P2-dependent raft-rich patches enhance local signaling for motility, and their assembly into clusters is regulated through captured MTs and PKA, coupling local regulation of motility to cell polarity, and organization.
Genome-wide analyses of small non-coding RNAs in streptococci

Streptococci represent a diverse group of Gram-positive bacteria, which colonize a wide range of hosts among animals and humans. Streptococcal species occur as commensal as well as pathogenic organisms. Many of the pathogenic species can cause severe, invasive infections in their hosts leading to a high morbidity and mortality. The consequence is a tremendous suffering on the part of men and livestock besides the significant financial burden in the agricultural and healthcare sectors. An environmentally stimulated and tightly controlled expression of virulence factor genes is of fundamental importance for streptococcal pathogenicity. Bacterial small non-coding RNAs (sRNAs) modulate the expression of genes involved in stress response, sugar metabolism, surface composition, and other properties that are related to bacterial virulence. Even though the regulatory character is shared by this class of RNAs, variation on the molecular level results in a high diversity of functional mechanisms. The knowledge about the role of sRNAs in streptococci is still limited, but in recent years, genome-wide screens for sRNAs have been conducted in an increasing number of species. Bioinformatics prediction approaches have been employed as well as expression analyses by classical array techniques or next generation sequencing. This review will give an overview of whole genome screens for sRNAs in streptococci with a focus on describing the different methods and comparing their outcome considering sRNA conservation among species, functional similarities, and relevance for streptococcal infection.
Simplification envelopes

We propose the idea of simpliﬁcation envelopes for generating a hierarchy of level-of-detail approximations for a given polygonal model. Our approach guarantees that all points of an approximation are within a user-speciﬁable distance (cid:15) from the original model and that all points of the original model are within a distance (cid:15) from the approximation. Simpliﬁcationenvelopes provide a general framework within which a large collection of existing simpliﬁcation algorithms can run. We demonstrate this technique in conjunction with two algorithms, one local, the other global. The local algorithm provides a fast method for generating approximations to large input meshes (at least hundreds of thousands of triangles). The global algorithm provides the opportunity to avoid local “minima” and possibly achieve better simpliﬁcations as a result. Each approximation attempts to minimize the total number of polygons required to satisfy the above (cid:15) constraint. The key advantages of our approach are:
Study on the Potential Toxicity of a Thymoquinone-Rich Fraction Nanoemulsion in Sprague Dawley Rats

Toxicological studies constitute an essential part of the effort in developing an herbal medicine into a drug product. A newly developed thymoquinone-rich fraction nanoemulsion (TQRFNE) has been prepared using a high pressure homogenizer. The purpose of this study was to investigate the potential acute toxicity of this nanoemulsion in Sprague Dawley rats. The acute toxicity studies were conducted as per the OECD guidelines 425, allowing for the use of test dose limit of 20 mL TQRFNE (containing 44.5 mg TQ)/kg. TQRFNE and distilled water (DW) as a control were administered orally to both sexes of rats on Day 0 and observed for 14 days. All the animals appeared normal, and healthy throughout the study. There was no observed mortality or any signs of toxicity during the experimental period. The effects of the TQRFNE and DW groups on general behavior, body weight, food and water consumption, relative organ weight, hematology, histopathology, and clinical biochemistry were measured. All the parameters measured were unaffected as compared to the control (DW) group. The administration of 20 mL TQRFNE /kg was not toxic after an acute exposure.
Can MRI Visual Assessment Differentiate the Variants of Primary-Progressive Aphasia?

BACKGROUND AND PURPOSE: Primary-progressive aphasia is a clinically and pathologically heterogeneous condition. Nonfluent, semantic, and logopenic are the currently recognized clinical variants. The recommendations for the classification of primary-progressive aphasia have advocated variant-specific patterns of atrophy. The aims of the present study were to evaluate the sensitivity and specificity of the proposed imaging criteria and to assess the intra- and interrater reporting agreements. MATERIALS AND METHODS: The cohort comprised 51 patients with a root diagnosis of primary-progressive aphasia, 25 patients with typical Alzheimer disease, and 26 matched control participants. Group-level analysis (voxel-based morphometry) confirmed the proposed atrophy patterns for the 3 syndromes. The individual T1-weighted anatomic images were reported by 3 senior neuroradiologists. RESULTS: We observed a dichotomized pattern of high sensitivity (92%) and specificity (93%) for the proposed atrophy pattern of semantic-variant primary-progressive aphasia and low sensitivity (21% for nonfluent-variant primary-progressive aphasia and 43% for logopenic-variant primary-progressive aphasia) but high specificity (91% for nonfluent-variant primary-progressive aphasia and 95% for logopenic-variant primary-progressive aphasia) in other primary-progressive aphasia variants and Alzheimer disease (sensitivity 43%, specificity 92%). MR imaging was least sensitive for the diagnosis of nonfluent-variant primary-progressive aphasia. Intrarater agreement analysis showed mean κ values above the widely accepted threshold of 0.6 (mean, 0.63 ± 0.16). Pair-wise interobserver agreement outcomes, however, were well below this threshold in 5 of the 6 possible interrater contrasts (mean, 0.41 ± 0.09). CONCLUSIONS: While the group-level results were in precise agreement with the recommendations, semantic-variant primary-progressive aphasia was the only subtype for which the proposed recommendations were both sensitive and specific at an individual level.
Mobile sales assistant: NFC for retailers

The Mobile Sales Assistant helps retailers and customers to check the availability of articles with any NFC enabled mobile phone at the Point of Sale. The application uses NFC tags attached to products or to warehouse shelves to identify the articles by Electronic Product Code (EPC). It sends a request containing EPC to a server application which is connected to the local and company-wide Enterprise Resource Planning (ERP) system. Information about the article is sent to the mobile phone display. This makes the shopping experience for the customer faster and more convenient.
Essays on Earning Management and Leading Indicator Variables

This dissertation consists of two essays. The first essay provides a rational explanation of the mysterious earnings discontinuity phenomenon. It also develops two new empirical predictions which are supported by the empirical tests. The second essay identifies conditions under which leading indicator variables discourage long-term investment. Essay 1: Discontinuity in Earnings Distribution: A Theory and Evidence This paper presents a rational model of financial reporting in which investors use reported earnings not only to infer true (pre-managed) earnings but also to update their beliefs about the precision (inverse of the variance) of earnings. In the model, overreporting earnings has two opposing pricing effects. For example, when earnings are positively auto-correlated, inflating a positive (reported) earnings surprise has a positive pricing effect because investors infer higher (pre-managed) earnings for both current and future periods. However, investors also infer a lower earnings precision from the higher earnings surprise, leading to a lower pricing weight placed on the higher surprise. This is the negative pricing effect of over-reporting earnings. For firms whose earnings are strongly positively auto-correlated, the trade-off between the two opposing effects creates a pooled report right above the prior mean of the earnings distribution and a no-reporting "hole" right below the prior mean in equilibrium (i.e., an earnings discontinuity around the prior mean). The pricing function of reported earnings exhibits an overall "S-shape" and a negative slope for medium (positive and negative) earnings surprises. The above theoretical results are consistent with existing empirical findings. What distinguish the paper are two new empirical predictions: (1) no earnings discontinuity exists for firms whose earnings are negatively or weakly positively auto-correlated, and (2) the earnings discontinuity is more pronounced for firms with more positively auto-correlated earnings (higher auto-covariance or lower variance). The paper also presents empirical evidence supporting the two predictions. Essay 2: When Leading Indicator Variables reduce Long-term Investment
How much reduction in portal pressure is necessary to prevent variceal rebleeding? a longitudinal study in 225 patients with transjugular intrahepatic portosystemic shunts

OBJECTIVES:This longitudinal study determines the risk of rebleeding in relation to the reduction of the portosystemic pressure gradient in patients with a transjugular intrahepatic portosystemic shunt (TIPS) for variceal bleeding.METHODS:The study included 225 patients in whom a TIPS revision was indicated by the endoscopic finding of varices with a high risk for rebleeding (n = 167) or a recent variceal rebleed (n = 58). The portosystemic pressure gradient was determined before and after TIPS placement and at revision performed after a mean of 10 ± 15 months.RESULTS:The portosystemic pressure gradient at revision approached the index pressure gradient before TIPS implantation (23.1 ± 5.5 mm Hg) by 8.4 ± 31%. Rebleeding was inversely correlated with the reduction in index pressure gradient found at revision. Thus, 80% of rebleedings occurred with pressure gradients close to the index pressure gradient (<25% reduction) or with gradients equal to or greater than the index pressure gradient. In contrast, only one patient (0.4%) and three patients (1.3%) rebled with a pressure gradient of <12 mm Hg or a reduction of the index pressure gradient by >50%, respectively. Kaplan-Meier analysis of rebleeding, which included the 225 patients at risk, showed a probability of rebleeding of 18%, 7%, and 1% for a reduction of the index pressure gradient by 0%, 25–50%, and >50%, respectively.CONCLUSIONS:Most rebleedings occurred with pressure gradients similar to the index-pressure gradient measured at first bleeding. Accordingly, a graded reduction by 25–50% sufficiently prevents rebleeding. It can be assumed that, in comparison with the widely used threshold value of 12 mm Hg, a reduction by 25–50% may have a favorable benefit-to-risk ratio with respect to shunt-induced hepatic encephalopathy and liver failure. It should therefore be a goal in the decompressive treatment of portal hypertension and maintained during follow-up of patients with variceal bleeding.
Estimation of human daily boron exposure in a boron-rich area

Although, the safe limits of human daily boron (B) exposure are not absolutely clear, there is a growing interest in B and its effects on human health. The aim of the present study was to estimate daily B exposure in 66 males in Turkey living in a B-rich area using water containing at least 2 mg/l boron, with an average age of 38·55 (se 1·66) years and an average number of years of residence in the B-rich area of 35·89 (se 1·73). Another group of males (n 57), living in the city centres of Balıkesir and Ankara, were taken as controls; the average age and number of years of residence for this group were 29·44 (se 1·43) and 10·26 (se 1·83) years, respectively. As it is assumed that the B level in urine reflects daily B exposure, the amount of urinary B of both the study and control groups was analysed by using an inductively coupled plasma optical emission spectrometry (ICP-OES) technique. The average daily B exposure value was calculated as 6·77 (se 0·47) mg in the study group and 1·26 (se 0·1) mg in the controls. The results of this study are expected to contribute to creating a reference value for a safe daily B exposure.