Miracle-dz/UniFAR-FTUs huggingface dataset:
ATP-Citrate Lyase Links Cellular Metabolism to Histone Acetylation

Chromatin Modifier Modulates Gene Expression Modification of chromatin structure is usually thought of as a global, relatively nonspecific way of modulating gene expression. However, Wellen et al. (p. 1076; see the Perspective by Rathmell and Newgard) demonstrate that such regulation helps link growth factor–stimulated increases in metabolism to appropriate changes in gene expression. Adenosine triphosphate (ATP)–citrate lyase (ACL), which converts citrate to acetyl–coenzyme A (CoA) in the mitochondria of mammalian cells during metabolism of glucose, was also found to be present in the nucleus, where it might regulate activity of histone acetyl transferases (HATs) by controlling the availability of acetyl-CoA. Indeed, depletion of ACL from cultured human colon carcinoma cells specifically decreased histone acetylation in the nucleus, but appeared not to affect the overall amount of acetylation of proteins in the cells. Loss of ACL in cultured mouse 3T3-L1 cells diminished the increase in histone acetylation normally associated with hormone-stimulated differentiation of these cells and inhibited the increase in expression of specific genes, such as that encoding the Glut4 glucose transporter. Thus, ACL may help cells link metabolic activity to changes in gene expression. Histone acetylation and gene expression in mammals are modulated by glycolytic metabolism. Histone acetylation in single-cell eukaryotes relies on acetyl coenzyme A (acetyl-CoA) synthetase enzymes that use acetate to produce acetyl-CoA. Metazoans, however, use glucose as their main carbon source and have exposure only to low concentrations of extracellular acetate. We have shown that histone acetylation in mammalian cells is dependent on adenosine triphosphate (ATP)–citrate lyase (ACL), the enzyme that converts glucose-derived citrate into acetyl-CoA. We found that ACL is required for increases in histone acetylation in response to growth factor stimulation and during differentiation, and that glucose availability can affect histone acetylation in an ACL-dependent manner. Together, these findings suggest that ACL activity is required to link growth factor–induced increases in nutrient metabolism to the regulation of histone acetylation and gene expression.
A viral suppressor of RNA silencing differentially regulates the accumulation of short interfering RNAs and micro-RNAs in tobacco

Two major classes of small noncoding RNAs have emerged as important regulators of gene expression in eukaryotes, the short interfering RNAs (siRNAs) associated with RNA silencing and endogenous micro-RNAs (miRNAs) implicated in regulation of gene expression. Helper component-proteinase (HC-Pro) is a viral protein that blocks RNA silencing in plants. Here we examine the effect of HC-Pro on the accumulation of siRNAs and endogenous miRNAs. siRNAs were analyzed in transgenic tobacco plants silenced in response to three different classes of transgenes: sense-transgenes, inverted-repeat transgenes, and amplicon-transgenes. HC-Pro suppressed silencing in each line, blocking accumulation of the associated siRNAs and allowing accumulation of transcripts from the previously silenced loci. HC-Pro-suppression of silencing in the inverted-repeat- and amplicon-transgenic lines was accompanied by the apparent accumulation of long double-stranded RNAs and proportional amounts of small RNAs that are larger than the siRNAs that accumulate during silencing. Analysis of these results suggests that HC-Pro interferes with silencing either by inhibiting siRNA processing from double-stranded RNA precursors or by destabilizing siRNAs. In contrast to siRNAs, the accumulation of endogenous miRNAs was greatly enhanced in all of the HC-Pro-expressing lines. Thus, our results demonstrate that accumulation of siRNAs and miRNAs in plants can be differentially regulated by a viral protein. The fact that HC-Pro affects the miRNA pathway raises the possibility that this pathway is targeted by plant viruses as a means to control gene expression in the host.
What Ecological and Anthropogenic Factors Affect Group Size in White‐lipped Peccaries (Tayassu pecari)?

Group living among ungulates has evolved mainly in species living in open habitats, such as grasslands and savannas, whereas in the forest, few ungulate species form groups and these tend to be small. Therefore, the white‐lipped peccary (Tayassu pecari), a Neotropical ungulate listed as Vulnerable by the IUCN, represents an almost unique social occurrence as it lives in large and cohesive groups, yet it inhabits dense tropical forests. Large variations in group sizes have been observed throughout the species range, with reports of herds with less than 10 to around 300 individuals. We examined factors that might cause variation in group size in white‐lipped peccary, including ecological and anthropogenic variables. We conducted an extensive literature review and used our original data to compile information on white‐lipped peccary's group size across its range. We built models to quantitate generalizations for group sizes distinguishing data from areas with high human influence, and areas that have not been significantly disturbed by humans for at least the last 20 years. We found that white‐lipped peccary's group size for all sites was most strongly predicted by a combination of the distances to the nearest human settlement and rainfall and its seasonality. Results from the undisturbed sites indicated that group size is positively influenced by rainfall. Our results contribute to understand why group size varies in different environments that are subjected to different ecological and human conditions. Information on these relationships is a key to advance our understanding of the socio‐ecological strategies of animal species living in groups.
Relationship between season, lactation number and incidence of clinical mastitis in different stages of lactation in a Holstein dairy farm

The aim of the present study was to compare the occurrence and duration of clinical mastitis in different seasons, stages of lactation period and parities in a Holstein dairy farm in Iran. A retrospective epidemiological survey from April 2005 to March 2008 was conducted on 884 clinical mastitis cases of 7437 lactations. Data of each case including calendar-date of mastitis onset, days in milk (DIM) of mastitis onset (early: 0-74 DIM; middle: 75-150 DIM, and late ≥ 150 DIM), duration of mastitis, and parity (1, 2, and ≥ 3) were recorded. Based on date of mastitis onset, cases were classified into stages of lactation. Moreover, beginning of mastitis was seasonally categorized. Duration of clinical mastitis after treatment in early lactation was less than late lactation in the first-parity cows (p = 0.005). In early lactation period, the first-parity cows suffered clinical mastitis in days earlier than two other parity groups (p < 0.001). Moreover, in late lactation period, the first-parity cows had clinical mastitis in days later than cows in the third and more parities (p = 0.002). Occurrence of clinical mastitis in summer increased in late lactation period but in winter increased in early lactation period (p = 0.001). In addition, occurrence time of clinical mastitis in summer were in days later than in spring (p = 0.02) and winter (p = 0.03) in early lactation period. In conclusion, occurrence of mastitis in winter and spring during early lactation and in summer during late lactation period were more prevalent especially in lower parities.
Domestic and Farm-Animal Exposures and Risk of Non-Hodgkin's Lymphoma in a Population-Based Study in the San Francisco Bay Area

Objective: To assess the association between animal exposures and non-Hodgkin's lymphoma (NHL). Methods: Exposure data were collected from 1,591 cases and 2,515 controls during in-person interviews in a population-based case-control study of NHL in the San Francisco Bay Area. Odds ratios (OR) and 95% confidence intervals (95% CI) were adjusted for potential confounders. Results: Pet owners had a reduced risk of NHL (OR, 0.71; 95% CI, 0.52-0.97) and diffuse large-cell lymphoma large cell (DLCL; OR, 0.58; 95% CI, 0.39-0.87) compared with those who never had owned a pet. Ever having owned dogs and/or cats was associated with reduced risk of all NHL (OR, 0.71; 95% CI, 0.54-0.94) and of DLCL (OR, 0.60; 95% CI, 0.42-0.86). Longer duration of cat ownership (Ptrend = 0.008), dog ownership (Ptrend = 0.04), and dog and/or cat ownership (Ptrend = 0.004) was inversely associated with risk of NHL. Ownership of pets other than cats and dogs was associated with a reduced risk of NHL (OR, 0.64; 95% CI, 0.55-0.74) and DLCL (OR, 0.58; 95% CI, 0.47-0.71). Exposure to cattle for ≥5 years was associated with an increased risk of NHL (OR, 1.6; 95% CI, 1.0-2.5) as was exposure to pigs for all NHL (OR, 1.8; 95% CI, 1.2-2.6) and for DLCL (OR, 2.0; 95% CI, 1.2-3.4). Conclusions: The association between animal exposure and NHL warrants further investigation in pooled analyses. (Cancer Epidemiol Biomarkers Prev 2008;17(9):2382–7)
Barriers in accessing care services for physically disabled in a hospital setting in Riyadh, Saudi Arabia, cross-sectional study

ABSTRACT Objective: The aim of this study was to identify the significant difficulties in access to health care services experienced by patients with physical disabilities. Method: A cross-sectional study at King Abdul Aziz Medical City, Riyadh, Saudi Arabia on 235 persons with physical disability, from 1 January to 30 June 2014. Results: A total of 88% of the people with disability reported having the need for someone to accompany them. Over 52% were unsatisfied with parking, 49.8% with the waiting area, 51.3% with wheel-chair services, and nearly 45% were unsatisfied with toilet facilities for the physically disabled. Those who were wheel-chair bound had statistically significant lower mean score for satisfaction with services and facilities related to parking area, reception, and appointment, elevator, and physiotherapy.The majority were satisfied with the attitude of the clinical staff. Conclusion: The majority of patients with physical disability require assistance and cannot move around independently in the healthcare facilities. Nearly half of these patients face several challenges in accessing health care services and are unsatisfied with the services received. Patients with physical disability who are wheel-chair bound have a lesser degree of satisfaction than those who are not wheel-chair bound.
Does transactional leadership count for team innovativeness

Purpose – Drawing on the contingency perspective of leadership, the purpose of this paper is to examine the relation between transactional leadership and team innovativeness by focusing on the moderating role of emotional labor and the mediating role of team efficacy. The authors propose and empirically test the hypothesis that the relation between transactional leadership and team innovativeness is negative when the level of emotional labor is high whereas the relation is positive when the level of emotional labor is low. The authors further examine the process mechanism of this interactive effect by testing the mediating role of team efficacy.Design/methodology/approach – In total, 90 Chinese work teams, comprising 462 members and 90 team leaders, were surveyed. Hierarchical regression analyses were performed and moderated causal steps approach applied to test the authors' mediated moderation model.Findings – As predicted, transactional leadership was negatively associated with team innovativeness when .
A controlled evaluation of a comprehensive mental skills program for Ncaa and youth club swimmers

A Controlled Evaluation of a Comprehensive Mental Skills Program for NCAA and Youth Club Swimmers By Kevin M. Lancer, M A. Dr. Brad Donohue, Examination Committee Chair Professor of Psychology University of Nevada, Las Vegas Previous studies have established that mental skills training is an effective intervention for improving performance in athletes. Relaxation, imagery, and mental preparation interventions have demonstrated efficacy in the improvement of athletic performance. However, relaxation combined with imagery is more effective than either one alone. Similarly, past studies have indicated mental preparation interventions may be more effective in the improvement of athletic performance when combined with other interventions. In team sports such as swimming, optimum performance has been found to improve cohesion. However, whether cohesion leads to improvements in sports performance is undetermined. In this study, a multiple baseline across team sports methodology was utilized to evaluate the effectiveness of cohesion and multi-component interventions in swim performance. The study included thirty-nine NCAA swimmers, and twelve Youth Club swimmers. Results indicated that the cohesion intervention appeared to enhance swim trial times for males, and female youth swimmers. However, this
Ligand binding‐dependent functions of the lipocalin NLaz: an in vivo study in Drosophila

Lipocalins are small extracellular proteins mostly described as lipid carriers. The Drosophila lipocalin NLaz (neural Lazarillo) modulates the IIS pathway and regulates longevity, stress resistance, and behavior. Here, we test whether a native hydrophobic pocket structure is required for NLaz to perform its functions. We use a point mutation altering the binding pocket (NLazL130R) and control mutations outside NLaz binding pocket. Tryptophan fluorescence titration reveals that NLazL130R loses its ability to bind ergosterol and the pheromone 7(z)‐tricosene but retains retinoic acid binding. Using site‐directed transgenesis in Drosophila, we test the functionality of the ligand binding‐altered lipocalin at the organism level. NLaz‐dependent life span reduction, oxidative stress and starvation sensitivity, aging markers accumulation, and deficient courtship are rescued by overexpression of NLazWT, but not of NLazL130R. Transcriptional responses to aging and oxidative stress show a large set of age‐responsive genes dependent on the integrity of NLaz binding pocket. Inhibition of IIS activity and modulation of oxidative stress and infection‐responsive genes are binding pocket‐dependent processes. Control of energy metabolites on starvation appears to be, however, insensitive to the modification of the NLaz binding pocket. —Ruiz, M. , Ganfornina, M. D. , Correnti, C. , Strong, R. K. , and Sanchez, D. Ligand binding‐dependent functions of the lipocalin NLaz: an in vivo study in Drosophila. FASEB J. 28, 1555–1567 (2014). www.fasebj. org
Accuracy of Anthropometric Measurements: Past, Present, and Future

Experience, based on anthropometric examination of over 1000 children with facial syndromes and more than 2400 healthy subjects of both sexes and various ages, has led me to diverge in some points from the more usual views found in the physical anthropologic literature. The purpose of this paper is to discuss the major controversial topics associated with anthropometric measurement. These include the problems associated with formation of a representative population sample, the relative validity of longitudinal and cross-sectional studies, the interpretation of intraobserver and interobserver testings, and the questionable judgments of mensurative skill in clinical practice. The factors influencing the accuracy of anthropometric measurements, definitions of both the consistent and less reliable measurements, and the duration of validity of anthropometric normative data are also discussed.
Perceived Job Discrimination in Australia: Its Correlates and Consequences

We use data from a nationally representative Australian household panel survey to examine the extent and nature of self-reported job discrimination, its correlates, and its associations with various employment outcomes and measures of subjective wellbeing. We find that approximately 8.5% of job applicants and 7.5% of employees report being discriminated against in the preceding two years, most commonly on the basis of their age. Gender is found to be a common factor predicting perceived discrimination in both job applications and in the course of employment, but the determinants of these two types of discrimination are otherwise somewhat different. In particular, age is a significant determinant of perceived discrimination in job applications only, while being a mother of young children is a significant factor only for discrimination in the course of employment. We also find that, holding other traits constant, ethnic and religious minorities are not significantly more likely to perceive they have been discriminated against. Little evidence of adverse effects of perceived job discrimination is found for wage levels, wage changes and the probability of promotion, but we find large negative effects on subjective outcomes such as job satisfaction and self-assessed probability of job loss.
Effect of Interactions between Harvester Ants on Forager Decisions

Harvester ant colonies adjust their foraging activity to day-to-day changes in food availability and hour-to-hour changes in environmental conditions. This collective behavior is regulated through interactions, in the form of brief antennal contacts, between outgoing foragers and returning foragers with food. Here we consider how an ant, waiting in the entrance chamber just inside the nest entrance, uses its accumulated experience of interactions to decide whether to leave the nest to forage. Using videos of field observations, we tracked the interactions and foraging decisions of ants in the entrance chamber. Outgoing foragers tended to interact with returning foragers at higher rates than ants that returned to the deeper nest and did not forage. To provide a mechanistic framework for interpreting these results, we develop a decision model in which ants make decisions based upon a noisy accumulation of individual contacts with returning foragers. The model can reproduce core trends and realistic distributions for individual ant interaction statistics, and suggests possible mechanisms by which foraging activity may be regulated at an individual ant level.
Querying socio-spatial networks on the world-wide web

navigation systems, allow users to record their location history. The location history data can be analyzed to generate life patterns|patterns that associate people to places they frequently visit. Accordingly, an SSN is a graph that consists of (1) a social network, (2) a spatial network, and (3) life patterns that connect the users of the social network to locations, i.e., to geographical entities in the spatial network. In this paper we present a system that stores SNN in a graph-based database management system and provides a novel query language, namely SSNQL, for querying the integrated data. The system includes a Web-based graphical user interface that allows presenting the social network, presenting the spatial network and posing SSNQL queries over the integrated data. The user interface also depicts the structure of queries for the purpose of debugging and optimization. Our demonstration presents the management of the integrated data as an SSN and it illustrates the query evaluation process in SSNQL.
UWB channel modelling for wireless body area networks in a hospital

This paper describes an experimental study of the ultra wideband (UWB) wireless body area network (WBAN) channel in a hospital environment. The measured data are used to develop statistical models for the channel, which can then be used to design efficient and safe communication networks. WBANs are expected to be used in the healthcare field to enable concepts such as telemedicine. The human body has a complex shape and consists of different tissues. It is then expected that the propagation of the electromagnetic waves near a patient's body will have unique characteristics. In a hospital environment, there are also a variety of electronic devices and specialised medical equipment, which have impact on the electromagnetic propagation. To properly design WBANs is then necessary to have a good understanding of the characteristics of the radio channel in the proximity of the human body within a hospital.
Altered Protein Expression in the Ileum of Mice Associated with the Development of Chronic Infections with Echinostoma caproni (Trematoda)

Background Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode that has been extensively used as experimental model to investigate the factors determining the expulsion of intestinal helminths or, in contrast, the development of chronic infections. Herein, we analyze the changes in protein expression induced by E. caproni infection in ICR mice, a host of high compatibility in which the parasites develop chronic infections. Methodology/Principal Findings To determine the changes in protein expression, a two-dimensional DIGE approach using protein extracts from the intestine of naïve and infected mice was employed; and spots showing significant differential expression were analyzed by mass spectrometry. A total of 37 spots were identified differentially expressed in infected mice (10 were found to be over-expressed and 27 down-regulated). These proteins were related to the restoration of the intestinal epithelium and the control of homeostatic dysregulation, concomitantly with mitochondrial and cytoskeletal proteins among others. Conclusion/Significance Our results suggests that changes in these processes in the ileal epithelium of ICR mice may facilitate the establishment of the parasite and the development of chronic infections. These results may serve to explain the factors determining the development of chronicity in intestinal helminth infection.
Sources and Consequences of Turkish Middle School Students’ Science Self-Efficacy

The present study aimed at investigating sources and consequences of Turkish middle school students’ science self-efficacy beliefs. While mastery experience, vicarious experience, verbal persuasion, and emotional arousal were examined as sources of self-efficacy beliefs, students’ achievement goals, metacognition, and effort regulation were examined as consequences of self-efficacy beliefs. Self-report instruments were administered to 1,932 middle school students to assess variables of the study. Results showed that mastery experience, verbal persuasion, and emotional arousal significantly predict students’ science self-efficacy which was found to be positively linked to achievement goals, metacognition, and effort regulation. In addition, a positive relationship was found between verbal persuasion and mastery approach goals. Moreover, findings revealed that approach goals were positively associated with metacognition and effort regulation while avoidance goals are negatively linked to effort regulation. Additionally, results indicated a positive association between emotional arousal and effort regulation.
Stabilizing novel objects by learning to predict tactile slip

During grasping and other in-hand manipulation tasks maintaining a stable grip on the object is crucial for the task's outcome. Inherently connected to grip stability is the concept of slip. Slip occurs when the contact between the fingertip and the object is partially lost, resulting in sudden undesired changes to the objects state. While several approaches for slip detection have been proposed in the literature, they frequently rely on previous knowledge of the manipulated object. This previous knowledge may be unavailable, seeing that robots operating in real-world scenarios often must interact with previously unseen objects. In our work we explore the generalization capabilities of well known supervised learning methods, using random forest classifiers to create generalizable slip predictors. We utilize these classifiers in the feedback loop of an object stabilization controller. We show that the controller can successfully stabilize previously unknown objects by predicting and counteracting slip events.