Miracle-dz/UniFAR-FTUs huggingface dataset:
Long-distance spore transport: methods of measurement, vertical spore profiles and the detection of immigrant spores.

SUMMARY: Airborne spores can be carried long distances, but little is known about the atmospheric transport processes involved or the rates at which spore clouds are depleted. Aircraft sampling is expensive and inevitably intermittent, and surface traps reveal only some of the processes involved. The best compromise is to combine surface and aircraft observations and to support both with detailed meteorological interpretation. Gravity slide traps exposed for 1 day indicate the arrival of spores less precisely than moving-slide impactors, which therefore provide a more accurate starting time for estimating the past track of spores from air trajectories. Catches of Puccinia graminis uredospores from continental European sources illustrated how immigration depends on the movement of atmospheric pressure systems and the gradients within them and suggested that in addition to surface air movement winds at the 700 and 500 mb. levels were important. Aircraft of the Meteorological Research Flight, using suction impactors which operated approximately isokinetically, sampled air in the lower troposphere, both to ascertain vertical spore profiles over land and to intercept immigrant Puccinia graminis uredospores over the English Channel. The vertical distribution of spores seemed to be determined in the same way as that of other aerosol particles; atmospheric turbulence was a major factor and there were indications that wind shear, precipitation and surface deposition might be important. However, most spores are liberated periodically and so encounter different degrees of atmospheric turbulence depending on the diurnal periodicity of their concentration near the ground. Concentrations of 104 spores/m.3 occurred at heights up to 1000 m. and hundreds/m.3 at 3000 m. In unstable air spore concentrations often declined roughly logarithmically with height, but layers of stable air were often associated with abrupt changes of concentration. Details of vertical spore profiles also depended on the history of both the temperature profile and the spore cloud. Such factors tended to affect all spore types similarly: but occasionally some components, e.g. P. graminis uredospores, showed unique vertical profiles. One such profile, characterized by preferential ‘erosion’ of the spore cloud from air near the surface, may indicate travel remote from sources. Spores of plant pathogenic fungi were frequent in samples of air moving northward over the English Channel but their viability was not tested.
“I have often walked down this street before”: fMRI Studies on the hippocampus and other structures during mental navigation of an old environment

The role of the hippocampus in recent spatial memory has been well documented in patients with damage to this structure, but there is now evidence that the hippocampus may not be needed for the storage and recovery of a spatial layout that was experienced long before injury. Such preservation may rely, instead, on a network of dissociable, extra‐hippocampal regions implicated in topographical orientation. Using functional magnetic resonance imaging (fMRI), we investigated this hypothesis in healthy individuals with extensive experience navigating in a large‐scale urban environment (downtown Toronto). Participants were scanned as they performed mental navigation tasks that emphasized different types of spatial representations. Tasks included proximity judgments, distance judgments, landmark sequencing, and blocked‐route problem‐solving. The following regions were engaged to varying degrees depending on the processing demands of each task: retrosplenial cortex, believed to be involved in assigning directional significance to locales within a relatively allocentric framework; medial and posterior parietal cortex, concerned with processing space within egocentric coordinates during imagined movement; and regions of prefrontal cortex, present in tasks heavily dependent on working memory. In a second, event‐related experiment, a distinct area of inferotemporal cortex was revealed during identification of familiar landmarks relative to unknown buildings in addition to activation of many of those regions identified in the navigation tasks. This result suggests that familiar landmarks are strongly integrated with the spatial context in which they were experienced. Importantly, right medial temporal lobe activity was observed, its magnitude equivalent across all tasks, though the core of the activated region was in the parahippocampal gyrus, barely touching the hippocampus proper. © 2004 Wiley‐Liss, Inc.
Efficient Genetic Method for Establishing Drosophila Cell Lines Unlocks the Potential to Create Lines of Specific Genotypes

Analysis of cells in culture has made substantial contributions to biological research. The versatility and scale of in vitro manipulation and new applications such as high-throughput gene silencing screens ensure the continued importance of cell-culture studies. In comparison to mammalian systems, Drosophila cell culture is underdeveloped, primarily because there is no general genetic method for deriving new cell lines. Here we found expression of the conserved oncogene RasV12 (a constitutively activated form of Ras) profoundly influences the development of primary cultures derived from embryos. The cultures become confluent in about three weeks and can be passaged with great success. The lines have undergone more than 90 population doublings and therefore constitute continuous cell lines. Most lines are composed of spindle-shaped cells of mesodermal type. We tested the use of the method for deriving Drosophila cell lines of a specific genotype by establishing cultures from embryos in which the warts (wts) tumor suppressor gene was targeted. We successfully created several cell lines and found that these differ from controls because they are primarily polyploid. This phenotype likely reflects the known role for the mammalian wts counterparts in the tetraploidy checkpoint. We conclude that expression of RasV12 is a powerful genetic mechanism to promote proliferation in Drosophila primary culture cells and serves as an efficient means to generate continuous cell lines of a given genotype.
Modulation of Cell Firing in the Nucleus Accumbens

ABSTRACT: Pennartz et al. 48 have proposed that functions of the nucleus accumbens (NA) are subserved by the activity of ensembles of neurons rather than by an overall neuronal activation. Indeed, the NA is a site of convergence for a large number of inputs from limbic structures that may modulate the flow of prefrontal cortical information and contribute to defining such ensembles, as exemplified in the ability of hippocampal input to gate cortical throughput in the nucleus accumbens. NA neurons exhibit a bistable membrane potential, characterized by a very negative resting membrane potential (down state), periodically interrupted by plateau depolarizations (up state), during which the cells may fire in response to cortical inputs. A dynamic ensemble can be the result of a distributed set of neurons in their up state, determined by the moment‐to‐moment changes in the spatial distribution of hippocampal inputs responsible for transitions to the up state. Ensembles may change as an adaptation to the contextual information provided by the hippocampal input. Furthermore, for dynamic ensembles to be functionally relevant, the model calls for near synchronous transitions to the up state in a group of neurons. This can be accomplished by the cell‐to‐cell transfer of information via gap junctions, a mechanism that can allow for a transfer of slow electrical signals, including “up” events between coupled cells. Furthermore, gap junction permeability is tightly modulated by a number of factors, including levels of dopamine and nitric oxide, and cortical inputs, allowing for fine‐tuning of this synchronization of up events. The continuous selection of such dynamic ensembles in the NA may be disputed in schizophrenia, resulting in an inappropriate level of activity of thalamocortical systems.
Plasmodium Strain Determines Dendritic Cell Function Essential for Survival from Malaria

The severity of malaria can range from asymptomatic to lethal infections involving severe anaemia and cerebral disease. However, the molecular and cellular factors responsible for these differences in disease severity are poorly understood. Identifying the factors that mediate virulence will contribute to developing antiparasitic immune responses. Since immunity is initiated by dendritic cells (DCs), we compared their phenotype and function following infection with either a nonlethal or lethal strain of the rodent parasite, Plasmodium yoelii, to identify their contribution to disease severity. DCs from nonlethal infections were fully functional and capable of secreting cytokines and stimulating T cells. In contrast, DCs from lethal infections were not functional. We then transferred DCs from mice with nonlethal infections to mice given lethal infections and showed that these DCs mediated control of parasitemia and survival. IL-12 was necessary for survival. To our knowledge, our studies have shown for the first time that during a malaria infection, DC function is essential for survival. More importantly, the functions of these DCs are determined by the strain of parasite. Our studies may explain, in part, why natural malaria infections may have different outcomes.
The Cinnamon-Derived Dietary Factor Cinnamic Aldehyde Activates the Nrf2-Dependent Antioxidant Response in Human Epithelial Colon Cells

Colorectal cancer (CRC) is a major cause of tumor-related morbidity and mortality worldwide. Recent research suggests that pharmacological intervention using dietary factors that activate the redox sensitive Nrf2/Keap1-ARE signaling pathway may represent a promising strategy for chemoprevention of human cancer including CRC. In our search for dietary Nrf2 activators with potential chemopreventive activity targeting CRC, we have focused our studies on trans-cinnamic aldehyde (cinnamaldeyde, CA), the key flavor compound in cinnamon essential oil. Here we demonstrate that CA and an ethanolic extract (CE) prepared from Cinnamomum cassia bark, standardized for CA content by GC-MS analysis, display equipotent activity as inducers of Nrf2 transcriptional activity. In human colon cancer cells (HCT116, HT29) and non-immortalized primary fetal colon cells (FHC), CA- and CE-treatment upregulated cellular protein levels of Nrf2 and established Nrf2 targets involved in the antioxidant response including heme oxygenase 1 (HO-1) and γ-glutamyl-cysteine synthetase (γ-GCS, catalytic subunit). CA- and CE-pretreatment strongly upregulated cellular glutathione levels and protected HCT116 cells against hydrogen peroxide-induced genotoxicity and arsenic-induced oxidative insult. Taken together our data demonstrate that the cinnamon-derived food factor CA is a potent activator of the Nrf2-orchestrated antioxidant response in cultured human epithelial colon cells. CA may therefore represent an underappreciated chemopreventive dietary factor targeting colorectal carcinogenesis.
Control Capacity of Partially Observable Dynamic Systems in Continuous Time

Stochastic dynamic control systems relate in a prob- abilistic fashion the space of control signals to the space of corresponding future states. Consequently, stochastic dynamic systems can be interpreted as an information channel between the control space and the state space. In this work we study this control-to-state informartion capacity of stochastic dynamic systems in continuous-time, when the states are observed only partially. The control-to-state capacity, known as empowerment, was shown in the past to be useful in solving various Artificial Intelligence & Control benchmarks, and was used to replace problem-specific utilities. The higher the value of empowerment is, the more optional future states an agent may reach by using its controls inside a given time horizon. The contribution of this work is that we derive an efficient solution for computing the control-to-state information capacity for a linear, partially-observed Gaussian dynamic control system in continuous time, and discover new relationships between control-theoretic and information-theoretic properties of dynamic systems. Particularly, using the derived method, we demonstrate that the capacity between the control signal and the system output does not grow without limits with the length of the control signal. This means that only the near-past window of the control signal contributes effectively to the control-to-state capacity, while most of the information beyond this window is irrelevant for the future state of the dynamic system. We show that empowerment depends on a time constant of a dynamic system.
Introducing Mentoring to 1st-year Medical Students of a Private Medical College in North India: A Pilot Study

Background: The stress of complex medical course, emotional immaturity, and adaptations to new surroundings are the challenges faced by the new medical entrants. Therefore, mentorship program was introduced to support them for their academic and personal development. Aim and Objectives: The aim of this study is to introduce and to assess the perception of mentors and mentees on mentorship program. Materials and Methods: A mentorship program was designed for Bachelor of Medicine and Bachelor of Surgery (MBBS) First Professional students. A 1-day workshop was conducted to sensitize the faculty. Seventeen faculty members from various departments volunteered to be mentors. After sensitization, 150 MBBS First Professional students were divided among these faculty members by lottery system. A regular visit of mentees was scheduled with the mentor. At the end of mentorship program, the perception of mentors and mentees was taken using a validated and semi-structured feedback questionnaire. A focus group discussion of students was also conducted. Results: A total of 112 students and 16 faculty members completed the feedback questionnaire. The mentors considered this program helpful in their self-improvement, teaching, and communication skills. Most of the mentees felt that this program helped them emotionally and academically. It was a good way to develop a strong student–teacher relationship. All the mentors and mentees were satisfied with the mentorship program. Conclusions: The newly introduced mentorship program helped in the overall development of mentors and mentees. Both mentors and mentees were extremely satisfied with this program and considered this as a successful intervention.
The Influence of Negative Emotion on the Simon Effect as Reflected by P300

The Simon effect refers to the phenomenon that reaction time (RT) is faster when stimulus and response location are congruent than when they are not. This study used the priming-target paradigm to explore the influence of induced negative emotion on the Simon effect with event-related potential techniques (ERPs). The priming stimuli were composed of two kinds of pictures, the negative and neutral pictures, selected from the International Affective Picture System (IAPS). The target stimuli included chessboards of two color types. One was red and black the other one was green and black. Each chessboard was presented on the left or the right of the screen. The participants were asked to press the response keys according to the colors of the chessboards. It was called the congruent condition if the chessboard and the response key were on the same side, otherwise incongruent condition. In this study, the emotion-priming Simon effect was found in terms of RT and P300. Negative emotion compared with neutral emotion significantly enhanced the Simon effect in the cognitive process, reflected by a larger difference of P300 latency between the incongruent and congruent trials. The results suggest that the induced negative emotion influenced the Simon effect at the late stage of the cognitive process, and the P300 latency could be considered as the reference measure. These findings may be beneficial to researches in psychology and industrial engineering in the future.
Modified pulse repetition coding boosting energy detector performance in low data rate systems

We consider ultra-wideband impulse radio (UWB-IR) low data rate (LDR) applications where a more complex cluster head (CH) communicates with many basic sensors nodes (SN). At receiver side, noncoherent energy detectors (ED) operating at low sampling clock, i.e., below 300 kHz, are focused. Drawback is that EDs suffer from significant performance losses with respect to coherent receivers. Pulse repetition coding (PRC) is a known solution to increase receiver performance at the expense of more transmit power. But in LDR systems known PRC is very inefficient due to the low receiver sampling clock. Boosting transmit power is not possible due to Federal Communications Commission's (FCC) power constraints. Hence, we present a modified PRC scheme solving this problem. Modified repetition coded binary pulse position modulation (MPRC-BPPM) fully exploits FCC power constraints and for EDs of fixed integration duration is optimal with respect to bit error rate (BER). Furthermore, MPRC-BPPM combined with ED outperforms SRAKE receivers at the expense of more transmit power and makes ED's performance robust against strong channel delay spread variations.
Chemotactic preferences govern competition and pattern formation in simulated two-strain microbial communities

Substrate competition is a common mode of microbial interaction in natural environments. While growth properties play an important and well-studied role in competition, we here focus on the influence of motility. In a simulated two-strain community populating a homogeneous two-dimensional environment, strains competed for a common substrate and only differed in their chemotactic preference, either responding more sensitively to a chemoattractant excreted by themselves or responding more sensitively to substrate. Starting from homogeneous distributions, three possible behaviors were observed depending on the competitors' chemotactic preferences: (i) distributions remained homogeneous, (ii) patterns formed but dissolved at a later time point, resulting in a shifted community composition, and (iii) patterns emerged and led to the extinction of one strain. When patterns formed, the more aggregating strain populated the core of microbial aggregates where starving conditions prevailed, while the less aggregating strain populated the more productive zones at the fringe or outside aggregates, leading to a competitive advantage of the less aggregating strain. The presence of a competitor was found to modulate a strain's behavior, either suppressing or promoting aggregate formation. This observation provides a potential mechanism by which an aggregated lifestyle might evolve even if it is initially disadvantageous. Adverse effects can be avoided as a competitor hinders aggregate formation by a strain which has just acquired this ability. The presented results highlight both, the importance of microbial motility for competition and pattern formation, and the importance of the temporal evolution, or history, of microbial communities when trying to explain an observed distribution.
The Importance of Job Training to Job Satisfaction of Older Workers

If job training has positive impacts on worker satisfaction, then job training can have desirable consequences for an organization that result both directly through its effects on productivity and indirectly through its effects on job satisfaction. Furthermore, the aging of the workforce implies that older workers will become increasingly important to firms and to the economy. This study, therefore, seeks to examine the relationship between job training and job satisfaction, focusing in particular on U.S. workers born in 1964 or earlier. The results of ordered logit regression analysis indicate that availability and quality of training received directly affect job satisfaction.
Chromosomal Mapping of Repetitive DNAs in the Grasshopper Abracris flavolineata Reveal Possible Ancestry of the B Chromosome and H3 Histone Spreading

Supernumerary chromosomes (B chromosomes) occur in approximately 15% of eukaryote species. Although these chromosomes have been extensively studied, knowledge concerning their specific molecular composition is lacking in most cases. The accumulation of repetitive DNAs is one remarkable characteristic of B chromosomes, and the occurrence of distinct types of multigene families, satellite DNAs and some transposable elements have been reported. Here, we describe the organization of repetitive DNAs in the A complement and B chromosome system in the grasshopper species Abracris flavolineata using classical cytogenetic techniques and FISH analysis using probes for five multigene families, telomeric repeats and repetitive C0t-1 DNA fractions. The 18S rRNA and H3 histone multigene families are highly variable and well distributed in A. flavolineata chromosomes, which contrasts with the conservation of U snRNA genes and less variable distribution of 5S rDNA sequences. The H3 histone gene was an extensively distributed with clusters occurring in all chromosomes. Repetitive DNAs were concentrated in C-positive regions, including the pericentromeric region and small chromosomal arms, with some occurrence in C-negative regions, but abundance was low in the B chromosome. Finally, the first demonstration of the U2 snRNA gene in B chromosomes in A. flavolineata may shed light on its possible origin. These results provide new information regarding chromosomal variability for repetitive DNAs in grasshoppers and the specific molecular composition of B chromosomes.
Phase III trial of epirubicin plus paclitaxel compared with epirubicin plus cyclophosphamide as first-line chemotherapy for metastatic breast cancer: United Kingdom National Cancer Research Institute trial AB01.

PURPOSE To compare the effectiveness and tolerability of epirubicin and paclitaxel (EP) with epirubicin and cyclophosphamide (EC) as first-line chemotherapy for metastatic breast cancer (MBC). PATIENTS AND METHODS Patients previously untreated with chemotherapy (except for adjuvant therapy) were randomly assigned to receive either EP (epirubicin 75 mg/m2 and paclitaxel 200 mg/m2) or EC (epirubicin 75 mg/m2 and cyclophosphamide 600 mg/m2) administered intravenously every 3 weeks for a maximum of six cycles. The primary outcome was progression-free survival; secondary outcome measures were overall survival, response rates, and toxicity. RESULTS Between 1996 and 1999, 705 patients (353 EP patients and 352 EC patients) underwent random assignment. Patient characteristics were well matched between the two groups, and 71% of patients received six cycles of treatment. Objective response rates were 65% for the EP group and 55% for the EC group (P = .015). At the time of analysis, 641 patients (91%) had died. Median progression-free survival time was 7.0 months for the EP group and 7.1 months for the EC group (hazard ratio = 1.07; 95% CI, 0.92 to 1.24; P = .41), and median overall survival time was 13 months for the EP group and 14 months for the EC group (hazard ratio = 1.02; 95% CI, 0.87 to 1.19; P = .8). EP patients, compared with EC patients, had more grade 3 and 4 mucositis (6% v 2%, respectively; P = .0006) and grade 3 and 4 neurotoxicity (5% v 1%, respectively; P < .0001). CONCLUSION In terms of progression-free survival and overall survival, there was no evidence of a difference between EP and EC. The data demonstrate no additional advantage to using EP instead of EC as first-line chemotherapy for MBC in taxane-naïve patients.