Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytokine Profiling in Chagas Disease: Towards Understanding the Association with Infecting Trypanosoma cruzi Discrete Typing Units (A BENEFIT TRIAL Sub-Study)

Background Chagas disease caused by the protozoan Trypanosoma cruzi is an important public health problem in Latin America. The immunological mechanisms involved in Chagas disease pathogenesis remain incompletely elucidated. The aim of this study was to explore cytokine profiles and their possible association to the infecting DTU and the pathogenesis of Chagas disease. Methods 109 sero-positive T. cruzi patients and 21 negative controls from Bolivia and Colombia, were included. Flow cytometry assays for 13 cytokines were conducted on human sera. Patients were divided into two groups: in one we compared the quantification of cytokines between patients with and without chronic cardiomyopathy; in second group we compared the levels of cytokines and the genetic variability of T. cruzi. Results Significant difference in anti-inflammatory and pro-inflammatory cytokines profiles was observed between the two groups cardiac and non-cardiac. Moreover, serum levels of IFN-γ, IL-12, IL-22 and IL-10 presented an association with the genetic variability of T.cruzi, with significant differences in TcI and mixed infections TcI/TcII. Conclusion Expression of anti-inflammatory and pro-inflammatory cytokines may play a relevant role in determining the clinical presentation of chronic patients with Chagas disease and suggests the occurrence of specific immune responses, probably associated to different T. cruzi DTUs.
Disruption of Coiled-coil Domains in Fer Protein-tyrosine Kinase Abolishes Trimerization but Not Kinase Activation*

The protein-tyrosine kinase Fer and the highly homologous proto-oncoprotein Fps/Fes are implicated in signaling from a variety of growth factor and cytokine receptors. Here we examine the molecular basis of Fer kinase activation with an emphasis on the role of oligomerization. We show that Fer forms trimers in vivoand that disruption of either the first or second coiled-coil domain abolishes oligomerization, suggesting a cooperative interaction between these two domains. Although Fps/Fes also forms homotypic oligomers, probably via homologous coiled-coil domains, no heterotypic interactions were observed between Fer and Fps/Fes. Incorporation of catalytically inactive Fer peptides into the oligomeric complex caused only mild reduction of wild type Fer kinase activity, suggesting that kinase-inactive Fer would not behave as a potent dominant negative. Although oligomerization of Fer can potentiate autophosphorylation intrans at three major phosphorylation sites, these residues can likely also be phosphorylated in cis. In contrast, the testis-specific FerT isomer does not oligomerize and is able to autophosphorylate in cis at two of the same three residues autophosphorylated in Fer. These results suggest that although oligomerization potentiates autophosphorylation intrans, this is apparently not necessary for Fer activation.
Rabies Exposures, Post-Exposure Prophylaxis and Deaths in a Region of Endemic Canine Rabies

Background Thousands of human deaths from rabies occur annually despite the availability of effective vaccines following exposure, and for disease control in the animal reservoir. Our aim was to assess risk factors associated with exposure and to determine why human deaths from endemic canine rabies still occur. Methods and Findings Contact tracing was used to gather data on rabies exposures, post-exposure prophylaxis (PEP) delivered and deaths in two rural districts in northwestern Tanzania from 2002 to 2006. Data on risk factors and the propensity to seek and complete courses of PEP was collected using questionnaires. Exposures varied from 6–141/100,000 per year. Risk of exposure to rabies was greater in an area with agropastoralist communities (and larger domestic dog populations) than an area with pastoralist communities. Children were at greater risk than adults of being exposed to rabies and of developing clinical signs. PEP dramatically reduced the risk of developing rabies (odds ratio [OR] 17.33, 95% confidence interval [CI] 6.39–60.83) and when PEP was not delivered the risks were higher in the pastoralist than the agro-pastoralist area (OR 6.12, 95% CI 2.60–14.58). Low socioeconomic class and distance to medical facilities lengthened delays before PEP delivery. Over 20% of rabies-exposed individuals did not seek medical treatment and were not documented in official records and <65% received PEP. Animal bite injury records were an accurate indicator of rabies exposure incidence. Conclusions Insufficient knowledge about rabies dangers and prevention, particularly prompt PEP, but also wound management, was the main cause of rabies deaths. Education, particularly in poor and marginalized communities, but also for medical and veterinary workers, would prevent future deaths.
Comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against Enterococcous faecalis in root canals; an in vitro study

Background: Enterococcus faecalis has been widely used as a valuable microbiological pathogen for in vitro studies due to its ability to successfully colonize the root canal in a biofilm-like style, invade dentinal tubules, and resist endodontic treatment procedures.The aim of this study was to compare the bactericidal efficacy of photodynamic therapy (PDT), 2% chlorhexidine, 2.5% NaOCl, and combination of PDT and 2.5% NaOCl against E. faecalis. Materials and Methods: Sixty single-rooted teeth had their canals contaminated with E. faecalis in brain heart infusion broth and were incubated for 48 hours.The canals were then subjected to 2% chlorhexidine, 2.5% NaOCl, PDT (red light emitting diode 625 nm+ Toludine Blue) and PDT + 2.5% NaOCl. Controls consisted of no treatment (positive control) and without inoculation of bacterium (negative control). Following treatment, the canal contents were sampled with sterile paper points.The samples were dispersed in transport medium, serially diluted, and cultured on blood agar to determine the number of colony forming units (CFU). Data were analyzed by Mann-Whitney U test at 5% significance level. The significance level for all analyses was set at P <. 05. Results: Combination of PDT and 2.5% NaOCl achieved maximum reduction in recovered viable bacteria, no viable bacteria was observed after treatment of PDT + 2.5% NaOCl. Conclusion: Combination of PDT and 2.5% NaOCl simultaneously is effective in the elimination of E. faecalis from dentinal tubules under the conditions of this study.
Model-Based Vasculature Extraction From Optical Fluorescence Cryomicrotome Images

The aim of this study was to develop a novel method to reconstruct 3-D coronary vasculature from cryomicrotome images, comprised of two distinct sets of data-fluorescent microsphere beads and coronary vasculature. Fluorescent beads and cast injected into the vasculature were separately imaged with different filter settings to obtain the microsphere and vascular data, respectively. To extract the vascular anatomy, light scattering in the tissue was modelled using a point spread function (PSF). The PSF was parametrized by optical tissue excitation and emission attenuation coefficients, which were estimated by fitting simulated images of microspheres convolved with the PSF model to the experimental microsphere images. These parameters were then applied within a new model-based method for vessel radius estimation. Current state-of-the-art radii estimation methods and the proposed model-based method were applied on vessel phantoms. In this validation study, the full-width half-maximum method of radii estimation, when performed on the raw data without correcting for the optical blurring, resulted in 42.9% error on average for the 170&nbsp;μm vessel. In comparison, the model-based method resulted in 0.6% error on average for the same phantom. Whole-organ porcine coronary vasculature was automatically reconstructed with the new model-based vascular extraction method.
Carotid Stiffness and Physical Activity in Elderly—A Short Report of the SAPALDIA 3 Cohort Study

Introduction Regular physical activity has been shown to reduce cardiovascular disease risk in the general population. While smaller studies in specified groups (highly trained versus untrained individuals) indicate a certain dose-dependent effect of physical activity on the reduction of carotid stiffness (an indicator of subclinical vascular disease), it is unclear whether this association is present in a representative sample. Thus, we investigated this question cross-sectionally in participants from the population-based Swiss Cohort Study on Air Pollution And Lung and Heart Diseases In Adults (SAPALDIA). Methods Self-reported total, moderate and vigorous physical activity and distensibility as a measure of local arterial stiffness among 1636 participants aged 50 to 81 years without clinically manifest diseases were evaluated. Mixed regression models were used to examine associations of physical activity intensity with distensibility. Results Vigorous physical activity, but not total nor moderate physical activity, was significantly associated with increased distensibility (= reduced carotid stiffness) in univariate analyses (percent change in the geometric mean and 95% confidence interval per 1 standard deviation increment in vigorous physical activity = 2.54 (0.69; 4.43), p<0.01; in total physical activity = 1.62 (-0.22; 3.50), p = 0.08; in moderate physical activity = 0.70 (-1.12; 2.56), p = 0.45). These associations disappeared when we additionally adjusted for age. Conclusion After adjustment for the most important confounders and risk factors, we found no evidence for an association of physical activity with carotid stiffness in the general middle aged to elderly population.
Wild bees enhance honey bees’ pollination of hybrid sunflower

Pollinators are required for producing 15–30% of the human food supply, and farmers rely on managed honey bees throughout the world to provide these services. Yet honey bees are not always the most efficient pollinators of all crops and are declining in various parts of the world. Crop pollination shortages are becoming increasingly common. We found that behavioral interactions between wild and honey bees increase the pollination efficiency of honey bees on hybrid sunflower up to 5-fold, effectively doubling honey bee pollination services on the average field. These indirect contributions caused by interspecific interactions between wild and honey bees were more than five times more important than the contributions wild bees make to sunflower pollination directly. Both proximity to natural habitat and crop planting practices were significantly correlated with pollination services provided directly and indirectly by wild bees. Our results suggest that conserving wild habitat at the landscape scale and altering selected farm management techniques could increase hybrid sunflower production. These findings also demonstrate the economic importance of interspecific interactions for ecosystem services and suggest that protecting wild bee populations can help buffer the human food supply from honey bee shortages.
Audit of referral and explant diagnoses in lung transplantation: a pathologic study of lungs removed for parenchymal disease.

BACKGROUND Lung transplantation is performed for an increasing range of pulmonary conditions in which the diagnosis is often clinical or based on limited biopsy material. Diagnosis may be made late in the course of the disease where specific features are no longer present. Posttransplantation complications and disease recurrence may relate to the primary disease, and accurate diagnosis is therefore essential. METHODS AND RESULTS A pathologic review of 183 explanted lungs over a 10-year period (heart-lung = 109, single lung = 65, double lung = 9) showed 29 significant discrepancies or additional features likely to effect outcome. The final pathologic diagnosis was cystic fibrosis (n = 66), emphysema (59), bronchiectasis (17), pulmonary fibrosis (19), sarcoidosis (10), Langerhans cell histiocytosis (3), pulmonary veno-occlusive disease (3), posttransplantation obliterative bronchiolitis (2), primary hemosiderosis (1), rheumatoid obliterative bronchiolitis (1), extrinsic allergic alveolitis (1), pneumoconiosis (1). Unsuspected diagnoses included tuberculosis (8) (four cases of which were active and in single lung recipients requiring antituberculous chemotherapy), sarcoidosis (9), (of which, six were unsuspected primary diagnoses and three were additional diagnoses), veno-occlusive disease (3), carcinoma (1), pneumoconiosis (1), and pulmonary fibrosis (2). Aspergillus infection (2) and bronchocentric granulomatosis (3) were found in patients with cystic fibrosis. One active tuberculosis case also showed an aspergilloma. Unsuspected infections requiring therapy in immunosuppressed patients and previously unsuspected sarcoidosis, which is known to recur in the graft, were the major novel diagnoses. Discrepancy rate was 12 of 65 in single lungs (19%) and 17 of 109 in heart-lungs (16%). CONCLUSIONS These results emphasize the need for accurate preoperative diagnosis especially when the similarly diseased native lung remains in situ.
Loss of nighttime blood pressure dipping as a risk factor for coronary artery calcification in nondialysis chronic kidney disease

Abstract Diurnal variations in blood pressure (BP) loss are closely associated with target organ damage and cardiovascular events. The quantity of coronary artery calcification (CAC) correlates with the atherosclerotic plaque burden, and an increased quantity indicates a substantially increased risk of cardiovascular events. This study investigated the nighttime diurnal variation in BP loss associated with CAC in patients with chronic kidney disease (CKD). Of the 1958 participants, we enrolled 722 participants with CKD without a history of acute coronary syndrome or symptomatic coronary artery disease. CAC was measured with computed tomography. BP was measured using 24-hour ambulatory BP monitoring. Central BP was measured using a SphygmoCor waveform analysis system. Participants with CAC had significantly higher 24-hour systolic, daytime systolic, and nighttime systolic ambulatory BP and central systolic BP. The percentage of participants with dipping loss was significantly higher among those with CAC. Multivariate logistic regression analysis indicated that dipping loss and dipping ratio were independently associated with CAC after adjusting for traditional and nontraditional cardiovascular risk factors and other BP parameters, including measurements of office-measured BP and central BP. The dipping status improved risk prediction for CAC after considering traditional risk factors and office-measured BP, using the net reclassification improvement and integrated discrimination improvement. Nighttime loss of diurnal variation in BP is an independent risk factor for CAC in CKD patients.
Release Mechanisms of Renin Vesicle Content

ly playing a role in granule formation, the glycosylation state of renin may also be important for mannose-6phosphate receptor-mediated uptake of renin or prorenin in other tissues, such as the heart (62, 93). Protogranules that are destined for the regulated secretory pathway pinch off from the trans-Golgi network. They contain prorenin and proteases, such as prohormone convertases (1, 5) and cathepsin B (53, 58), that have the ability to cleave off the prosegment. Other proteases, such as kallikrein and plasmin, are also able to cleave the prosegment, but there is no evidence that they play a physiological role in the process. In dense core secretory granules, an acidic intracellular pH is created by vacuolar H+-ATPases. The low pH is optimal for the protease activity that activates prorenin to renin by splitting off the 43amino acid NH2-terminal propeptide. Activation of the proteases from their own pro-forms by autoactivation is also dependent on a low pH of 4–6. The low pH of the renin secretory vesicles has been used experimentally to stain them with substances like the fluorescent dye quinacrin (1, 9, 63). When using quinacrine for dynamic visualization of live cells, it is possible to observe disappearance of fluorescence from individual secretory granules, representing exocytosis, a sudden increase in granule pH, or both (63). The early secretory granules have the ability to take up extracellular markers, such as horseradish peroxidise, which is targeted to early granules without transit through the Golgi apparatus (88). Such retrograde transport linking early/recycling endosomes to the trans-Golgi network is a well known cellular phenomenon (e.g., Ref. 2) involving SNARE complexes and clathrin (74). The direct uptake route into the secretory granules could potentially be used to target the activation of renin.
Making computers look the way we look: exploiting visual attention for image understanding

Human Visual attention (HVA) is an important strategy to focus on specific information while observing and understanding visual stimuli. HVA involves making a series of fixations on select locations while performing tasks such as object recognition, scene understanding, etc. We present one of the first works that combines fixation information with automated concept detectors to (i) infer abstract image semantics, and (ii) enhance performance of object detectors. We develop visual attention-based models that sample fixation distributions and fixation transition distributions in regions-of-interest (ROI) to infer abstract semantics such as expressive faces and interactions (such as look, read, etc.). We also exploit eye-gaze information to deduce possible locations and scale of salient concepts and aid state-of-art detectors. A 18% performance increase with over 80% reduction in computational time for a state-of-art object detector [4].
Parameters Identification and Modeling of High-Frequency Current Transducer for Partial Discharge Measurements

Rogowski coil (RC) is a low-cost, air-cored, and flexible induction sensor for nonintrusive condition monitoring and thus can be used in a variety of applications. In this paper, a lumped parameter model of RC is presented and an experiment-based methodology is developed to determine its parameters. The performance of the RC is analyzed for detection and measurement of high-frequency (pulsed) signals such as partial discharge (PD) current pulses. A simple and efficient technique of numerical integration is adopted to avoid the conventional type of expensive and complex design analogue integrators. RC is modeled and simulated in the alternative transient program-electromagnetic transient program environment. The designed coil is tested to measure PDs in the laboratory. Simulated and experimental performance of RC is compared with a high-frequency current transformer. This comparison shows a good match and, hence, validates the design of RC for PD applications.
Still talking to machines (cognitively speaking)

This overview article reviews the structure of a fully statistical spoken dialogue system (SDS), using as illustration, various systems and components built at Cambridge over the last few years. Most of the components in an SDS are essentially classifiers which can be trained using supervised learning. However, the dialogue management component must track the state of the dialogue and optimise a reward accumulated over time. This requires techniques for statistical inference and policy optimisation using reinforcement learning. The potential advantages of a fully statistical SDS are the ability to train from data without hand-crafting, increased robustness to environmental noise and user uncertainty, and the ability to adapt and learn on-line.
Teaching Business Management to Engineers: The Impact of Interactive Lectures

Some education specialists are challenging the use of traditional strategies in classrooms and are calling for the use of contemporary teaching and learning techniques. In response to these calls, many field experiments that compare different teaching and learning strategies have been conducted. However, to date, little is known on the outcomes of these strategies specific to the context of engineers and engineering education. The debate on traditional versus contemporary strategies continues, with many traditionalists holding firm to the view that contemporary techniques diminish the academic quality and integrity of teaching and learning. This paper adds to the current debate by assessing the outcome of interactive lectures, a contemporary teaching strategy, in the context of engineering education. Specifically, this paper examines the effect of interactive lectures on student academic achievement and the learning environment. This paper supported the quantitative survey responses with self-reported student reflections. The findings contradict the traditionalist view of teaching and learning; student performance was not significantly different when interactive learning strategies were employed. However, students held a more positive view toward the learning environment and felt that interactive learning enhanced their overall learning experiences and spurred interest in the subject area. This paper concluded that interactive lecturing can be an effective approach in teaching business management in engineering education.
The Chinese Childbirth Self-Efficacy Inventory: the development of a short form.

AIMS AND OBJECTIVES To examine the validity and reliability of the short form of the Childbirth Self-Efficacy Inventory in a clinical Chinese pregnant population. BACKGROUND Childbirth self-efficacy has become an important psychological construct for both childbirth educators and researchers because pregnant women's perception may influence the meaning and consequences of childbirth. Assessment done by the original Childbirth Self-Efficacy Inventory limits the scientific rigor for generating additional research into the construct, as its items were repetitive and its structure is lengthy. METHODS This prospective study investigated 293 Chinese pregnant women in their third trimester of pregnancy. The women completed the short form of the Childbirth Self-Efficacy Inventory, the Chinese Self-efficacy Scale and the socio-demographic questionnaire in an interview room of the study hospital. The re-testing of the scale on the participants was carried out two weeks later in the same clinic. RESULTS Factor analysis and item-subscale correlations supported the conceptual dimensions of the short form of the Chinese Childbirth Self-Efficacy Inventory. The Cronbach's alpha coefficients for both subscales were over 0.9. The convergent validity with the Chinese Self-Efficacy Scale was reflected by a moderate correlation for the two subscales. The efficacy expectancy subscale differentiated primigravid from multigravid women (t = 2.83, P < 0.01, CI = -2.33 to 2.34). CONCLUSIONS The reliability and validity information presented in this paper supports the use of the short form of the Childbirth Self-Efficacy Inventory as a research instrument in measuring the childbirth self-efficacy among the Chinese population. Further validation of the measure is warranted. RELEVANCE TO CLINICAL PRACTICE The reliable short form of the Childbirth Self-Efficacy Inventory could be used in routine clinical practice in maternity care services to provide a point of reference for future research and development in both childbirth education and clinical practice.