Miracle-dz/UniFAR-FTUs huggingface dataset:
Inactivation of Glycogen Synthase Kinase 3 Promotes Axonal Growth and Recovery in the CNS

Axonal regeneration is minimal after CNS injuries in adult mammals and medical treatments to recover neurological deficits caused by axon disconnection are extremely limited. The failure of axonal elongation is principally attributed to the nonpermissive environment and reduced intrinsic growth capacity. In this report, we studied the role of glycogen synthase kinase-3 (GSK-3) inactivation on neurite and axon growth from adult neurons via combined in vitro and in vivo approaches. We found that the major CNS inhibiting substrates including chondroitin sulfate proteoglycans could inactivate protein kinase B (Akt) and activate GSK-3β signals in neurons. GSK-3 inactivation with pharmacologic inhibitors enhances neurite outgrowth of dorsal root ganglion neurons derived from adult mice or cerebellar granule neurons from postnatal rodents cultured on CNS inhibitors. Application of GSK-3 inhibitors stimulates axon formation and elongation of mature neurons whether in presence or absence of inhibitory substrates. Systemic application of the GSK-3 inhibitor lithium to spinal cord-lesioned rats suppresses the activity of this kinase around lesion. Treatments with GSK-3 inhibitors including a clinical dose of lithium to rats with thoracic spinal cord transection or contusion injuries induce significant descending corticospinal and serotonergic axon sprouting in caudal spinal cord and promote locomotor functional recovery. Our studies suggest that GSK-3 signal is an important therapeutic target for promoting functional recovery of adult CNS injuries and that administration of GSK-3 inhibitors may facilitate the development of an effective treatment to white matter injuries including spinal cord trauma given the wide use of lithium in humans.
Deficits in the Provision of Cardiopulmonary Resuscitation During Simulated Obstetric Crises: Results from the Israeli Board of Anesthesiologists

BACKGROUND:Cardiac arrest in the parturient is often fatal, but appropriate resuscitation in this special situation may save the lives of the mother and/or unborn baby. Concern has arisen as to application of recommended techniques for resuscitation in the obstetric patient. The Israel Board of Anesthesiology has incorporated simulation assessment into accreditation examinations. The candidates represent a unique national cohort in which we were able to assess competence in the simulated scenario of cardiorespiratory arrest in the parturient. METHODSA simulated scenario of preeclampsia with magnesium toxicity leading to cardiac arrest in a pregnant patient was performed by 25 senior anesthesiology residents. A unique two-stage simulation examination consisting of high fidelity simulation followed immediately by oral debriefing was conducted. The assessment was scored using a predetermined checklist of key actions and answers to clarifying questions. Simulation performance was compared to debriefing performance. RESULTSDuring the board examination, resuscitation not specific to the pregnant patient was performed well (commencing chest compressions, bag-mask ventilation, cardiac defibrillation); however actions specific to the parturient were performed poorly. Left uterine displacement, cricoid pressure during bag-mask ventilation, and instructing preparations to be made for perimortem cesarean delivery within 5 minutes were performed by 68%, 48%, and 40% of candidates respectively (lower 99% confidence limit 42%, 25%, and 19%, respectively). Cricoid pressure during bag-mask ventilation was performed by 48% (25%) but described in debriefing by 80% of candidates (53%) (P = 0.08), and time setting for perimortem cesarean delivery was performed by 40% (29%) but described by 80% (53%) (P = 0.05) of examinees. CONCLUSIONSSenior anesthesiology residents have poor knowledge of resuscitation of the pregnant patient. The results suggest 2-stage simulation including an oral component may reveal disparities in knowledge not assessed by simulation alone, but definitive conclusions require further study.
Few Mutations in the 5′ Leader Region Mediate Fitness Recovery of Debilitated Human Immunodeficiency Type 1 Viruses

ABSTRACT Repeated bottleneck passages of RNA viruses result in fitness losses due to the accumulation of deleterious mutations. In contrast, repeated transfers of large virus populations result in exponential fitness increases. Human immunodeficiency virus type 1 (HIV-1) manifested a drastic fitness loss after a limited number of plaque-to-plaque transfers in MT-4 cells. An analysis of the mutations associated with fitness loss in four debilitated clones revealed mutation frequencies in gag that were threefold higher than those in env. We now show an increase in the fitness of the debilitated HIV-1 clones by repeated passages of large populations. An analysis of the entire genomic nucleotide sequences of these populations showed that few mutations, from two to seven per clone, mediated fitness recovery. Eight of the 20 mutations affected coding regions, mainly by the introduction of nonsynonymous mutations (75%). However, most of the mutations accumulated during fitness recovery (12 of 20) were located in the 5′ untranslated leader region of the genome, and more specifically, in the primer binding site (PBS) loop. Two of the viruses incorporated the same mutation in the primer activation signal in the PBS loop, which is critical for the tRNA3Lys-mediated initiation of reverse transcription. Moreover, 25% of the mutations observed were reversions. This fact, together with the presence of a large proportion of nonsynonymous replacements, may disclose the operation, during large population passages, of strong positive selection for optimal HIV-1 replication, which seems to be primarily affected by binding of the tRNA to the PBS and the initiation of reverse transcription.
Inhibition of Cyclooxygenase-2 by Celecoxib Reverses Tumor-Induced Wasting

There have been a number of reports suggesting inhibition of prostaglandin production may impact tumor-mediated wasting and levels of associated humoral factors such as hypercalcemia. These reductions were achieved using traditional nonsteroidal anti-inflammatory drugs (NSAIDs), which are often contraindicated in cancer patients. This is especially true during chemotherapeutic regimens due to concerns of bleeding from gastrointestinal and hematopoietic toxicities associated with inhibition of the housekeeping cyclooxygenase enzyme COX-1. Here, we report that celecoxib, one of the new class of selective COX-2 inhibitors, has the potential to reverse tumor-mediated wasting and associated humoral factors such as interleukin (IL)-6 and hypercalcemia in preclinical models of cachexia. Tumor bearing mice in late stage cachexia regained weight within days of the start of celecoxib treatment. Two models were tested. The first was the Colon 26 (Col26) syngeneic murine model that induces high levels of circulating IL-6 and hypercalcemia. The second was the human head and neck 1483 HNSCC xenograft model, which is less inflammatory and produces less prostaglandin than Col26. Despite the observation that no significant impact on tumor growth was observed between vehicle and celecoxib-treated animals over the course of the studies, celecoxib rapidly reversed weight loss in both cachectic models. With the added safety of celecoxib over traditional NSAIDs, these results suggest a possible therapeutic use for celecoxib for treating tumor-mediated wasting.
Document heading doi : In vitro evaluation of free radical scavenging activity of Codariocalyx motorius root extract

In vitro evaluation of free radical scavenging activity of Codariocalyx motorius root extract Uma Chidambaram, Vanitha Pachamuthu, Suganya Natarajan, Bhakkiyalakshmi Elango, Suriyanarayanan, Kunga Mohan Ramkumar 1 Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur603 203, Tamilnadu, India 2 Department of Water and Health, JSS University, Mysore 570015, Karnataka, India
Ligand Activation of TAM Family Receptors-Implications for Tumor Biology and Therapeutic Response

The TAM family of receptors (i.e., Tyro3, Axl, and Mertk), and their ligands Growth arrest specific factor 6 (Gas6) and Protein S (Pros1) contribute to several oncogenic processes, such as cell survival, invasion, migration, chemo-resistance, and metastasis, whereby expression often correlates with poor clinical outcomes. In recent years, there has been great interest in the study of TAM receptors in cancer, stemming both from their roles as oncogenic signaling receptors, as well as their roles in tumor immunology. As a result, several classes of TAM inhibitors that include small molecule tyrosine kinase inhibitors, monoclonal antibodies, decoy receptors, as well as novel strategies to target TAM ligands are being developed. This paper will review the biology of TAM receptors and their ligands with a focus on cancer, as well as evidence-based data for the continued pursuit of TAM/Gas6 inhibitors in clinical practice.
Dose-dependent protection of reseveratrol against spinal cord ischemic-reperfusion injury in rats

Purpose : To examine the protective effects of resveratrol (RESV) against spinal cord ischemic reperfusion (SCIR) injury. Methods : Forty-eight male rats were divided into six groups: sham-operated (control-I), SCIR-treated (SCIR-II), rats receiving 20 mg/kg of RESV with SCIR (RESV 20+SCIR-III), rats receiving 40 mg/kg of RESV with SCIR (RESV 40+SCIR-IV), rats receiving 60 mg/kg of RESV with SCIR (RESV 60+SCIR-V), and rats receiving 50 mg/kg of methylprednisolone (MP) with SCIR (MP + SCIR-VI), for 7 days prior to IR (pre-treatment) and 7 days after IR (post-treatment). Results : The levels of oxidative markers (TBARS, MPO) and inflammatory markers (IL-1β, IL-6, TNF-α, and NF-p65) were concomitantly suppressed in RESV-treated rats, which showed improved locomotor function. A pronounced increase in the activities of antioxidant enzymes (SOD, CAT and GSH) was noted in the RESV group compared with the MP and SCIR groups. RESV and MP supplementation increased neuronal count with decreased nuclear degeneration. RESV (40 mg) exhibited greater protective effect than 20 mg and 60 mg of RESV and 50 mg of MP. Conclusion : The results show the neurotherapeutic potential of RESV (40 mg) to attenuate oxidative stress and the inflammatory response to SCIR injury. Keywords : Spinal cord ischemia reperfusion, Resveratrol, Locomotor function, Antioxidant, Inflammatory markers
Modeling and constraining human interactions in shared control utilizing a discrete event framework

Human integration is essential in systems where autonomous control alone would not be successful. In this paper, we present a framework for integrating a human supervisor into an otherwise autonomous control system. To facilitate integration, discrete event systems theory is adopted to model human interactions. It is via this interaction model that human commands can be combined with commands from an automated control system. For control synthesis, a method based on constraints is being used to generate velocity commands from the autonomous task level controller. The constraints are also utilized to limit human input so that erroneous human input is minimized. The methods are demonstrated by experiments.
Recycled Aggregate Concrete under Cyclic Loading

The objective of this paper was to evaluate the behavior of reinforced concrete beam-column joints that were subjected to cyclic loading in order to investigate and compare ductility and toughness of the joints made either of ordinary or recycled-aggregate concrete. Therefore, a recycled-aggregate concrete was prepared with water/cement of 0.35 and fluid consistency, by completely replacing natural aggregates with recycled aggregates coming from an industrial crushing plant in which rubble from building demolition was suitably treated. A reference concrete was also prepared using natural aggregates with the same fluid consistency but a water/cement of 0.60, sufficient to achieve adequate strength class. A further recycled-aggregate concrete was prepared with a water/cement of 0.40, by adding fly ash to the mixture as a partial replacement for fine aggregate. An acrylic polymer based superplasticiser was used to guarantee the same workability level. Concrete specimens were manufacture for the evaluation of compressive and tensile strength, as well as bond strength between concrete and reinforcing steel bars, to assess the suitability for structural uses. Then cyclic loading of the beam-column joints made of these concretes was carried out. The results gave further experimental evidence of the suitability of recycled-aggregate concrete for structural use, at least for ordinary reinforced concrete structures.
Understanding the Performance of Sparse Matrix-Vector Multiplication

In this paper we revisit the performance issues of the widely used sparse matrix-vector multiplication (SpMxV) kernel on modern microarchitectures. Previous scientific work reports a number of different factors that may significantly reduce performance. However, the interaction of these factors with the underlying architectural characteristics is not clearly understood, a fact that may lead to misguided and thus unsuccessful attempts for optimization. In order to gain an insight on the details of SpMxV performance, we conduct a suite of experiments on a rich set of matrices for three different commodity hardware platforms. Based on our experiments we extract useful conclusions that can serve as guidelines for the subsequent optimization process of the kernel.
An aquatic insect imaging system to automate insect classification

Population counts of aquatic insects are a valuable tool for monitoring the water quality of rivers and streams. However, the handling of samples in the lab for species identification is time consuming and requires specially trained experts. An aquatic insect imaging system was designed as part of a system to automate aquatic insect classification and was tested using several species and size classes of stonefly (Plecoptera). The system uses ethanol to transport specimens via a transparent rectangular tube to a digital camera. A small jet is used to position and reorient the specimens so that sufficient pictures can be taken to classify them with pattern recognition. A mirror system is used to provide a split set of images 90° apart. The system is evaluated with respect to engineering requirements developed during the research, including image quality, specimen handling, and system usability.
A double-blind controlled study of adjunctive treatment with risperidone in schizophrenic patients partially responsive to clozapine: efficacy and safety.

BACKGROUND Several open trials and case studies have reported beneficial effects following the addition of risperidone for partial responders to clozapine. The purpose of this study was to carry out a placebo-controlled, randomized, double-blind trial of the efficacy, safety, and tolerability of adjunctive treatment with risperidone in patients with schizophrenia partially responsive to clozapine. METHOD In this 6-week double-blind study, 30 patients with DSM-IV schizophrenia who had partial response to clozapine despite being treated for a mean of 32 months were randomly assigned to risperidone (N = 16) up to 6 mg/day or placebo (N = 14). Efficacy assessments included the Positive and Negative Syndrome Scale (PANSS), the Calgary Depression Scale, the Clinical Global Impressions-Severity of Illness scale, the Global Assessment of Functioning scale, and the Quality of Life Scale. A variety of safety and tolerability measures were also obtained. Data were collected between November 2001 and July 2003. RESULTS Significant improvement was noted in both groups on a variety of measures of psychopathology, but there was significantly greater improvement in the placebo-treated patients on the primary outcome measure, the PANSS positive symptom subscale. There were no significant differences between the treatment groups regarding extrapyramidal symptoms, weight gain, vital signs, serum clozapine levels, and QTc interval. The only side effect significantly more severe in risperidone-treated compared to placebo-treated patients was sedation. The patients treated with risperidone developed significant increases in plasma prolactin levels. CONCLUSION Adjunctive risperidone treatment in schizophrenia patients partially responsive to clozapine does not significantly improve psychopathology or quality of life compared to placebo in a 6-week period.
PUFA diets alter the microRNA expression profiles in an inflammation rat model

Omega-3 and -6 polyunsaturated fatty acids (PUFAs) can directly or indirectly regulate immune homeostasis via inflammatory pathways, and components of these pathways are crucial targets of microRNAs (miRNAs). However, no study has examined the changes in the miRNA transcriptome during PUFA-regulated inflammatory processes. Here, we established PUFA diet-induced autoimmune-prone (AP) and autoimmune-averse (AA) rat models, and studied their physical characteristics and immune status. Additionally, miRNA expression patterns in the rat models were compared using microarray assays and bioinformatic methods. A total of 54 miRNAs were differentially expressed in common between the AP and the AA rats, and the changes in rno-miR-19b-3p, -146b-5p and -183-5p expression were validated using stem-loop reverse transcription-quantitative polymerase chain reaction. To better understand the mechanisms underlying PUFA-regulated miRNA changes during inflammation, computational algorithms and biological databases were used to identify the target genes of the three validated miRNAs. Furthermore, Gene Ontology (GO) term annotation and KEGG pathway analyses of the miRNA targets further allowed to explore the potential implication of the miRNAs in inflammatory pathways. The predicted PUFA-regulated inflammatory pathways included the Toll-like receptor (TLR), T cell receptor (TCR), NOD-like receptor (NLR), RIG-I-like receptor (RLR), mitogen-activated protein kinase (MAPK) and the transforming growth factor-β (TGF-β) pathway. This study is the first report, to the best of our knowledge, on in vivo comparative profiling of miRNA transcriptomes in PUFA diet-induced inflammatory rat models using a microarray approach. The results provide a useful resource for future investigation of the role of PUFA-regulated miRNAs in immune homeostasis.
Development of a Fabry-Perot Etalon spectrometer for high-resolution aerosol observations in the oxygen A-band

Aerosols are an important factor of the Earth climatic system and they play a key role for air quality and public health. Observations of the Oxygen A-Band at 760 nm can provide information on the vertical distribution of aerosols from passive sensors, that can be of great interest for operational monitoring applications with high spatial coverage if the aerosol information is obtained with sufficient precision, accuracy and vertical resolution. To address this issue, retrieval simulations of the aerosol vertical profile retrieval from Oxygen A-Band observations by GOSAT, the upcoming OCO-2 and Sentinel 5-P mission and the proposed CarbonSat mission have been carried out. These simulations suggest that such observations will be useful in the measurement of uplifted aerosols such as volcanic plumes and forest fires. Retrievals from GOSAT were also undertaken that showed promising results in challenging conditions, warranting further study. The Fabry-Pérot Etalon spectrometer provides a compact, robust and affordable alternative to grating or Fourier transform spectrometers. Such an instrument was designed and optimised for the measurement of aerosol in the Oxygen A-Band and its performance simulated in comparison to GOSAT, where it competed well despite its low cost. Initial calibrations of the instrument were undertaken and showed promising results in the instrument operation concept. Following on from this simulations were used to study the performance of groundbased observations showing that using and combining a range of viewing angles would contribute significantly to the vertical information content whereas polarisation proved not to be useful except possibly in extreme circumstances.
Roughness sense display representing temporal frequency changes of tactile information in response to hand movements

We propose a hypothesis on mechanism of roughness perception based on temporal frequency changes of tactile information. Roughness sense can be considered as relative information to the hand velocity. Finite element analysis using human finger model showed that spatial information of the rough surface can be reflected in the temporal frequency changes at the position of tactile receptors. It should be important for roughness sense display to make a vibratory stimulation changing frequencies in response to the hand velocities.
SLAW : A Mobility Model for Human Walks

Simulating human mobility is important in mobile networks because many mobile devices are either attached to or controlled by humans and it is very hard to deploy real mobile networks whose size is controllably scalable for performance evaluation. Lately various measurement studies of human walk traces have discovered several significant statistical patterns of human mobility. Namely these include truncated power-law distributions of flights, pause-times and inter-contact times, fractal way-points, and heterogeneously defined areas of individual mobility. Unfortunately, none of existing mobility models effectively captures all of these features. This paper presents a new mobility model called SLAW (Self-similar Least Action Walk) that can produce synthetic walk traces containing all these features. This is by far the first such model. Our performance study using using SLAW generated traces indicates that SLAW is effective in representing social contexts present among people sharing common interests or those in a single community such as university campus, companies and theme parks. The social contexts are typically common gathering places where most people visit during their daily lives such as student unions, dormitory, street malls and restaurants. SLAW expresses the mobility patterns involving these contexts by fractal waypoints and heavy-tail flights on top of the waypoints. We verify through simulation that SLAW brings out the unique performance features of various mobile network routing protocols.