Miracle-dz/UniFAR-FTUs huggingface dataset:
Placental zinc in normal and intra-uterine growth-retarded pregnancies

The zinc concentration of placental tissue and cord blood in sixteen mothers who gave birth to normal babies was measured. The blood volume of each placenta was estimated from measurements of haemoglobin concentration of placental homogenate and cord blood, and, by deduction, the Zn content of blood-free placental tissue was calculated. Results were compared with eleven mothers whose fetuses showed a low biparietal diameter velocity between 17 and 28 weeks gestation and with ten mothers who gave birth to intra-uterine growth-retarded (IUGR) babies. As expected, placental weight was significantly correlated with infant birth weight. Blood-free placental tissue contained about four times xmore Zn (approximately 10 µg Zn/g) than cord blood (approximately 2.5 µg Zn/ml). Concentrations of Zn in blood-free placental tissue were similar in all three groups, but the cord blood Zn of mothers producing IUGR babies was significantly lower than that of the other two groups. Results of the present study suggested that fetal growth retardation in the mothers studied could not be explained by differences in blood-free placental Zn concentration, but that there may be some association between lower cord blood Zn levels and intra-uterine growth retardation.
Effects of PNPLA3 on Liver Fat and Metabolic Profile in Hispanic Children and Adolescents

OBJECTIVE A genome-wide study of adults identified a variant of PNPLA3 (rs738409) associated with ∼twofold higher liver fat. The purpose of this study was to examine the influence of PNPLA3 genotype on liver fat and other related metabolic outcomes in obese Hispanic children and adolescents. RESEARCH DESIGN AND METHODS Three hundred and twenty-seven Hispanics aged 8–18 years were genotyped for rs738409. One hundred and eighty-eight subjects had measures of visceral (VAT) and subcutaneous (SAT) adipose tissue volume and hepatic (HFF) and pancreatic (PFF) fat fraction by magnetic resonance imaging. One hundred and thirty-nine subjects did not have HFF measures but had extensive measures of insulin sensitivity and fasting lipids. RESULTS Liver fat in GG subjects was 1.7 and 2.4 times higher than GC and CC (11.1 ± 0.8% in GG vs. 6.6 ± 0.7% in GC and 4.7 ± 0.9% in CC; P < 0.0001), and this effect was observed even in the youngest children (8–10 years of age). The variant was not associated with VAT, SAT, PFF, or insulin sensitivity or other glucose/insulin indexes. However, Hispanic children carrying the GG genotype had significantly lower HDL cholesterol (40.9 ± 10.9 in CC vs. 37.0 ± 8.3 in CG vs. 35.7 ± 7.4 in GG; P = 0.03) and a tendency toward lower free fatty acid levels (P = 0.06). CONCLUSIONS These results provide new evidence that the effect of the PNPLA3 variant is apparent in Hispanic children and adolescents, is unique to fat deposition in liver as compared with other ectopic depots examined, and is associated with lower HDL cholesterol.
Substitutions at the Putative Receptor-Binding Site of an Encephalitic Flavivirus Alter Virulence and Host Cell Tropism and Reveal a Role for Glycosaminoglycans in Entry

ABSTRACT The flavivirus receptor-binding domain has been putatively assigned to a hydrophilic region (FG loop) in the envelope (E) protein. In some flaviviruses this domain harbors the integrin-binding motif Arg-Gly-Asp (RGD). One of us has shown earlier that host cell adaptation of Murray Valley encephalitis virus (MVE) can result in the selection of attenuated variants altered at E protein residue Asp390, which is part of an RGD motif. Here, a full-length, infectious cDNA clone of MVE was constructed and employed to systematically investigate the impact of single amino acid changes at Asp390 on cell tropism, virus entry, and virulence. Each of 10 different E protein 390 mutants was viable. Three mutants (Gly390, Ala390, and His390) showed pronounced differences from an infectious clone-derived control virus in growth in mammalian and mosquito cells. The altered cell tropism correlated with (i) a difference in entry kinetics, (ii) an increased dependence on glycosaminoglycans (determined by inhibition of virus infectivity by heparin) for attachment of the three mutants to different mammalian cells, and (iii) the loss of virulence in mice. These results confirm a functional role of the FG loop in the flavivirus E protein in virus entry and suggest that encephalitic flaviviruses can enter cells via attachment to glycosaminoglycans. However, it appears that additional cell surface molecules are also used as receptors by natural isolates of MVE and that the increased dependence on glycosaminoglycans for entry results in the loss of neuroinvasiveness.
Time for capnography – everywhere

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Numerical Simulations of Convective Accretion Flows in Three Dimensions

Advection-dominated accretion flows (ADAFs) are known to be convectively unstable for low values of the viscosity parameter α. Two-dimensional axisymmetric hydrodynamic simulations of such flows reveal a radial density profile that is significantly flatter than the ρ ∝ r-3/2 expected for a canonical ADAF. The modified density profile is the result of inward transport of angular momentum by axisymmetric convective eddies. We present three-dimensional hydrodynamic simulations of convective ADAFs that are free of the assumption of axisymmetry. We find that the results are qualitatively and quantitatively similar to those obtained with two-dimensional simulations. In particular, the convective eddies are nearly axisymmetric and transport angular momentum inward.
Hierarchical test pattern generation based on high-level primitives

It is demonstrated that the exploitation of high-level primitives (HLPs) and, in particular, of the knowledge concerning their function in ATPG (automatic test pattern generation) leads to significant improvements in implication, unique sensitization, and multiple backtrace. Motivated by this observation and the necessity of covering all faults inside HLPs, the authors present the extension of the ATPG system SOCRATES to hierarchical test pattern generation, which is based upon HLPs and the strategy of dynamically expanding the HLPs to their gate-level realization, at most one at a time. Experimental results have substantiated that the proposed approach performs significantly better in terms of CPU time, elapsed time, fault coverage, and memory requirements than a gate-level ATPG algorithm. It is expected that the extended SOCRATES algorithm will be capable of coping with circuits consisting of 100000 gates and more within reasonable times, even in a workstation environment. <<ETX>>
Game Design Patterns

We present a model to support the design, analysis, and comparison of games through the use of game design patterns, descriptions of reoccurring interaction relevant to game play. The model consists of a structural framework to describe the components of games, and patterns of interaction that describes how components are used by players (or a computer) to affect various aspects of the game play. Focusing on the patterns and identified methods for using them, we describe the development of the model and how we are currently working to enlarge and validate the collection of patterns.
The Relationship between National Culture and the Usability of an E-learning System

The purpose of this study was to investigate possible relationships between national culture and the usability of an e-learning system. The theoretical frameworks that were used to guide this study were Hofstede's cultural dimensions and Nielsen's usability attributes. The sample for this study was composed of the twenty-four attendees in an international workshop on training improvement held in Penang, Malaysia. This population was selected for this study because of the attendees' diverse cultural backgrounds and their underlying interest in all forms of training and instructional delivery. Three instruments were used in this study and each instrument assisted in collecting information regarding unique aspects of the research study. The study revealed that high uncertainty avoidance cultures found the e-learning system most frustrating to use. The study also found that individuals from cultures with low power distance indicators (e.g. people who are more accepting of uneven power distribution) rated the overall usability higher than individuals from high power distance indicator cultures.
Improved Robust Stability Criteria for Delayed Cellular Neural Networks via the LMI Approach

Uniqueness and robust exponential stability are analyzed for a class of uncertain cellular neural networks with time-varying delays. By dividing the variation interval of the time delay into two subintervals with equal length, a novel Lyapunov-Krasovskii functional is introduced. Using the free-weighting matrix method, a new delay-dependent stability criterion is obtained, which is less conservative than some previous literature. Since the result is presented in terms of linear matrix inequalities, the condition is easy to be verified. Finally, an example is given to illustrate the effectiveness of our proposed method.
Low Complexity Antenna Selection based MIMO Scheduling Algorithms for Uplink Multiuser MIMO/FDD System

Antenna selection schemes combined with the scheduler are applied to the uplink multiuser MIMO/FDD system. In this paper, two heuristic Antenna selection based MIMO schedulers are proposed to achieve the optimal performance with affordable complexity, where two search methods are also discussed to set up the antenna channels to be the candidates of the scheduler. Simulation results show that the sum rate and BER performance of the proposed low complexity schemes are about the same to that of the brute-force scheduler which is believed to be the optimal scheme. Moreover, the complexity can be further reduced by the sub-set search method when the number of transmit and receive antennas are 2 which is applicable in the realistic systems.
Phylogenetic Reconstruction of the Legionella pneumophila Philadelphia-1 Laboratory Strains through Comparative Genomics

Over 20 years ago, two groups independently domesticated Legionella pneumophila from a clinical isolate of bacteria collected during the first recognized outbreak of Legionnaires’ disease (at the 1976 American Legion’s convention in Philadelphia). These two laboratory strains, JR32 and Lp01, along with their derivatives, have been disseminated to a number of laboratories around the world and form the cornerstone of much of the research conducted on this important pathogen to date. Nevertheless, no exhaustive examination of the genetic distance between these strains and their clinical progenitor has been performed thus far. Such information is of paramount importance for making sense of several phenotypic differences observed between these strains. As environmental replication of L. pneumophila is thought to exclusively occur within natural protozoan hosts, retrospective analysis of the domestication and axenic culture of the Philadelphia-1 progenitor strain by two independent groups also provides an excellent opportunity to uncover evidence of adaptation to the laboratory environment. To reconstruct the phylogenetic relationships between the common laboratory strains of L. pneumophila Philadelphia-1 and their clinical ancestor, we performed whole-genome Illumina resequencing of the two founders of each laboratory lineage: JR32 and Lp01. As expected from earlier, targeted studies, Lp01 and JR32 contain large deletions in the lvh and tra regions, respectively. By sequencing additional strains derived from Lp01 (Lp02 and Lp03), we retraced the phylogeny of these strains relative to their reported ancestor, thereby reconstructing the evolutionary dynamics of each laboratory lineage from genomic data.
Clinical Effects of Regional Intravenous Guanethidine (Ismelin®) in Reflex Sympathetic Dystrophy

Twenty patients with documented reflex sympathetic dystrophy were treated with a series of regional intravenous guanethidine blocks. The mean delay between the first clinical symptoms and the start of guanethidine blocks was 3.6 months. The overall result was good in 11 patients, moderate in two patients and poor in seven patients. Poor results are due mainly to incorrect diagnosis and to application either too late in the third phase or too early in the first phase when only signs of increased blood flow are part of the symptomatology. Side effects, except pain after the injection, were few and of minor importance. The tolerance of the procedure may be improved by preceding the injection of guanethidine by an injection of a local anaesthetic agent. It may be concluded that with correct diagnosis and indication, guanethidine injections may play an important part in the treatment of reflex sympathetic dystrophy and may replace sympathetic blocks with local anaesthetics because of the longer duration of action and lower incidence of serious side‐effects.
Identifying racial differences in nodular lymphocyte‐predominant Hodgkin lymphoma

Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is an uncommon, indolent lymphoma comprising less than 10% of all new diagnoses of Hodgkin lymphoma (HL). In “Race-specific features and outcomes of nodular lymphocyte-predominant Hodgkin lymphoma: analysis of the National Cancer Data Base”, Dr. Olszewski and colleagues present the first comprehensive analysis of racial disparities in NLPHL. 1 This entity is a predominantly male disease with a 3:1 male to female ratio and a 1.2:1 ratio in African Americans compared to Caucasians. 2 NLPHL displays a bimodal age distribution with peaks in childhood and early adulthood with a median age similar to classical HL of approximately 30 years, but bears more similarity clinically to many subtypes of non-Hodgkin lymphoma (NHL) than to classical HL. 3 Because of the low frequency of this diagnosis there are few prospective clinical studies to guide clinical management producing controversies and conflicting data regarding the optimal management. This lack of consensus complicates our understanding of whether racial differences in patient management represent variations that may portend a worse outcome. The indolent
Genetic Ablation of Angiotensinogen in the Subfornical Organ of the Brain Prevents the Central Angiotensinergic Pressor Response

The subfornical organ (SFO) of the brain has long been considered a critical integrating center for the cardiovascular actions of the renin-angiotensin system (RAS). Early reports of angiotensin II (Ang II) immunoreactivity in the SFO and its neural projections to downstream cardiovascular nuclei raised the possibility that Ang II is produced locally and functions as a putative neurotransmitter in these circuits. However, evidence of functionally significant de novo synthesis of Ang II in the SFO has been lacking. Here, implementing spatiotemporally restricted gene ablation by way of the Cre recombinase/loxP system, we provide the first direct evidence that the local RAS in the SFO has a critical role in blood pressure regulation. Using a transgenic mouse harboring an angiotensinogen (AGT) gene modified for Cre-mediated deletion (hAGTflox), in combination with gene transfer of an adenovirus encoding Cre targeted to the SFO, we show that deletion of the Ang II substrate in this brain region nearly abolishes the pressor and bradycardic effects of renin infused in the CNS. Immunohistochemical analyses verified intense and restricted expression of Cre in the SFO, which paralleled the decrease in AGT expression selectively in this site. Further physiological studies confirmed the integrity of central angiotensinergic and nonangiotensinergic cardiovascular response systems in the Cre-treated mice. In addition to establishing that AGT expression in the SFO and its local conversion to Ang II has a profound effect on blood pressure, this study provides proof-of-principle of the utility of this approach for dissecting the brain RAS and other complex systems in CNS cardiovascular circuits.
Prevalence of COPD in Greece.

OBJECTIVES The prevalence of COPD in Greece is unknown. The aim of this study was to determine the prevalence and trends of COPD among adults in Greece. METHODS This study involved 888 randomly identified adults (475 men and 413 women) aged > 35 years, and smokers of at least 100 cigarettes, in a population-based, multiregional, cross-sectional descriptive design. The selected individuals reflected the urban/rural population distribution in Greece and, within each study region, the age group and gender of the community setting. The diagnosis of COPD was based on clinical and spirometric data including reversibility test (DeltaFEV(1) < 15%). RESULTS The overall prevalence of COPD in the population aged > 35 years with a smoking history of > 100 cigarettes per lifetime was 8.4%. The gender-standardized COPD prevalence was 11.6% for men and 4.8% for women. The COPD prevalence by community setting was as follows: Athens, 6%; other urban areas, 10.1%; semiurban areas, 8.5%; and rural areas, 9.1%. Smoking intensity and age were significantly associated with higher COPD prevalence in both men and women. CONCLUSIONS COPD is a substantial health problem in Greece, although prevalence rates are lower than expected when the high smoking rates are taken into account. The high proportion of the patients with mild COPD who were unaware of their illness highlights the need to increase public awareness of COPD.
Sequential tumor biopsies in early phase clinical trials of anticancer agents for pharmacodynamic evaluation.

PURPOSE In the setting of target-based anticancer drug development, it is critical to establish that the observed preclinical activity can be attributed to modulation of the intended target in early phase trials in human subjects. This paradigm of target modulation allows us to determine a Phase II or III dose (optimal biochemical/biological modulatory dose) that may not necessarily be the maximum tolerated dose. A major obstacle to target-based (often cytostatic) drug development has been obtaining relevant tumor tissue during clinical trials of these novel agents for laboratory analysis of the putative marker of drug effect. EXPERIMENTAL DESIGN From 1989 to present, we have completed seven clinical trials in which the end point was a biochemical or biological modulatory dose in human tumor tissues (not surrogate tissue). Eligibility enrollment required that patients have a biopsiable lesion either with computerized tomography (CT) guidance or direct visualization and consent to sequential (pre and posttreatment) biopsies. RESULTS A total of 192 biopsies were performed in 107 patients. All but 8 patients had sequential pre and posttreatment biopsies. Seventy-eight (73%) of the 107 patients had liver lesion biopsies. In eight patients, either one or both biopsies contained insufficient viable tumor tissue or no tumor tissue at all for analysis. Of a total of 99 patients in whom we attempted to obtain paired biopsies, a total of 87 (88%) were successful. Reasons for failure included patient refusal for a second biopsy (n = 2), vasovagal reaction with first biopsy precluding a second biopsy (n = 1), subcapsular hepatic bleeding (n = 1), and most commonly obtaining necrotic tumor, fibrous, or normal tissue in one of the two sequential biopsies (n = 8). CONCLUSIONS This is the first and largest reported series demonstrating that with adequate precautions and experience, sequential tumor biopsies are feasible and safe during early phase clinical trials.
Type I IFN Signaling Is Crucial for Host Resistance against Different Species of Pathogenic Bacteria1

It is known that host cells can produce type I IFNs (IFN-αβ) after exposure to conserved bacterial products, but the functional consequences of such responses on the outcome of bacterial infections are incompletely understood. We show in this study that IFN-αβ signaling is crucial for host defenses against different bacteria, including group B streptococci (GBS), pneumococci, and Escherichia coli. In response to GBS challenge, most mice lacking either the IFN-αβR or IFN-β died from unrestrained bacteremia, whereas all wild-type controls survived. The effect of IFN-αβR deficiency was marked, with mortality surpassing that seen in IFN-γR-deficient mice. Animals lacking both IFN-αβR and IFN-γR displayed additive lethality, suggesting that the two IFN types have complementary and nonredundant roles in host defenses. Increased production of IFN-αβ was detected in macrophages after exposure to GBS. Moreover, in the absence of IFN-αβ signaling, a marked reduction in macrophage production of IFN-γ, NO, and TNF-α was observed after stimulation with live bacteria or with purified LPS. Collectively, our data document a novel, fundamental function of IFN-αβ in boosting macrophage responses and host resistance against bacterial pathogens. These data may be useful to devise alternative strategies to treat bacterial infections.
Fractional Genetic Programming with Probability Density Data

We extend the fractional genetic programming scheme with data elements that are no more scalar, but instead are similar to probability density functions. The extension straightforwardly fits into fractional programming, in which data elements are blended from several values. In the case of our previous work, the blend produced a single scalar value. The extension proposes to build an approximate probability density function out of the blended elements. The extension turned out to be very effective in an unsuspected way: when a data element, despite being destined to approximate a probability density, represented a single–dimensional image of spatial data.
Rethinking the initialization bias problem in steady-state discrete event simulation

The state in which a discrete event simulation is started causes the estimators for equilibrium measures obtained from the simulation to be biased, and to reduce this bias, the collection of data is delayed until a so-called warm-up period is completed. In this paper, we determine the optimal warm-up periods for steady-state discrete event simulations. We do this by using deterministic numerical methods, that is, methods not using random numbers. We found that in the systems investigated, transient expectations give no indication regarding the optimal length of the warm-up periods, which is counterintuitive. This requires some re-evaluation of some of commonly held opinions about the factors one should take into account when determining warm-up periods. Such factors will also be discussed.