Miracle-dz/UniFAR-FTUs huggingface dataset:
Development of a Next-Generation Environmental Chamber Facility for Chemical Mechanism and VOC Reactivity Research

A new state-of-the-art indoor environmental chamber facility for the study of atmospheric processes leading to the formation of ozone and secondary organic aerosol (SOA) has been constructed and characterized. The chamber is designed for atmospheric chemical mechanism evaluation at low reactant concentrations under well-controlled environmental conditions. It consists of two collapsible 90 m3 FEP Teflon film reactors on pressure-controlled moveable frameworks inside a temperature-controlled enclosure flushed with purified air. Solar radiation is simulated with either a 200 kW Argon arc lamp or multiple blacklamps. Results of initial characterization experiments, all carried out under dry conditions, concerning NOx and formaldehyde offgasing, radical sources, particle loss rates, and background PM formation are described. Results of initial single organic - NOx and simplified ambient surrogate - NOx experiments to demonstrate the utility of the facility for mechanism evaluation under low NOx conditions are summarized and compared with the predictions of the SAPRC-99 chemical mechanism. Overall, the results of the initial characterization and evaluation indicate that this new environmental chamber can provide high quality mechanism evaluation data for experiments with NOx levels as low as ~2 ppb, though the results indicate some problems with the gas-phase mechanism that need further study. Initial evaluation experiments for SOA formation, also carried out under dry conditions, indicate that the chamber can provide high quality secondary aerosol formation data at relatively low hydrocarbon concentrations.
A Cisplatin and vinorelbine (NP) regimen as a postoperative adjuvant chemotherapy for completely resected breast cancers in China: final results of a phase II clinical trial.

OBJECTIVE To evaluate the efficacy and toxicity of cisplatin and vinorelbine (NP) for postoperative adjuvant chemotherapy of completely resected breast cancers. METHODS Between September 1994 and April 2005, 91 Chinese breast cancer patients, with pathologically-confirmed adenocarcinoma in Jiangsu Cancer Hospital and Research Institute, were enrolled. They received postoperative vinorelbine at 25 mg/m² on days 1 and 8, and cisplatin 25mg/m2 on days 1 to 3, this regimen being repeated every 3 weeks. RESULTS Median age was 49 years (range, 25-69 years). According to the TNM stage system, stage I, II, IIIA patients accounted for 7.7%, 58.2% and 34.1%, respectively. The median number of chemotherapy cycles was 4.5 (range, 1-8), over half of the patients receiving 4 to 6 NP cycles. After a median follow-up of 48 months, 11 deaths and 29 relapses were documented. Median disease-free survival was 45 months, with disease-free and overall survival at 5 years being 76% and 88.7%, respectively. All patients could be evaluated with regard to toxicity, 17 (18.7%) developing grade III neutropenia during treatment, but all recovering after recombinant human granulocyte colony stimulating factor (G-CSF) injection, 3 suffering thrombocytopenia (3.3%), 5 anemia (5.5%) and 5 nausea/vomiting (5.5%). No treatment related deaths occurred. CONCLUSIONS NP is an effective and feasible treatment for completely resected breast cancer cases at the doses tested. A randomized clinical trial is now needed to compare NP with other conventional regimens.
A monoclonal antibody directed against the 97-kilodalton gonococcal hemin-binding protein inhibits hemin utilization by Neisseria gonorrhoeae

Neisseria gonorrhoeae expresses two hemin-binding proteins (HmBPs) of 97,000 and 44,000 in molecular weight. A murine monoclonal antibody (MAb) produced against the 97-kDa HmBP from N. gonorrhoeae PID543 specifically inhibited in a concentration-dependent manner the ability of hemin to promote growth. The anti-97-kDa HmBP MAb competitively inhibited binding of the 97-kDa HmBP to a hemin-agarose affinity column. In Western immunoblots, the MAb recognized the 97-kDa homologs from a limited survey of clinical gonococcal isolates. These results support the contention that the 97-kDa HmBP is involved in the gonococcal hemin acquisition pathway.
Extracellular and Intracellular Mechanisms of Mechanotransduction in Three-Dimensionally Embedded Rat Chondrocytes

Purpose Articular cartilage homeostasis involves modulation of chondrocyte matrix synthesis in response to mechanical stress (MS). We studied extracellular and intracellular mechanotransduction pathways mediating this response. Methods We first confirmed rapid up-regulation of the putative chondro-protective cytokine, interleukin (IL)-4, as an immediate response to MS. We then studied the role of IL-4 by investigating responses to exogenous IL-4 or a specific IL-4 inhibitor, combined with MS. Next we investigated the intracellular second messengers. Since chondrocyte phenotype alters according to the extracellular environment, we characterized the response to mechanotransduction in 3-dimensionally embedded chondrocytes. Results Expression of aggrecan and type II collagen was significantly up-regulated by exogenous IL-4 whereas MS-induced matrix synthesis was inhibited by an IL-4 blocker. Further, MS-induced matrix synthesis was completely blocked by a p38 MAPK inhibitor, while it was only partially blocked by inhibitors of other putative second messengers. Conclusion IL-4 mediates an extracellular pathway of mechanotransduction, perhaps via an autocrine/paracrine loop, while p38 mediates an intracellular pathway prevalent only in a 3-dimensional environment.
Epigenetic alterations in hippocampus of SAMP8 senescent mice and modulation by voluntary physical exercise

The senescence-accelerated SAMP8 mouse model displays features of cognitive decline and Alzheimer's disease. With the purpose of identifying potential epigenetic markers involved in aging and neurodegeneration, here we analyzed the expression of 84 mature miRNAs, the expression of histone-acetylation regulatory genes and the global histone acetylation in the hippocampus of 8-month-old SAMP8 mice, using SAMR1 mice as control. We also examined the modulation of these parameters by 8 weeks of voluntary exercise. Twenty-one miRNAs were differentially expressed between sedentary SAMP8 and SAMR1 mice and seven miRNAs were responsive to exercise in both strains. SAMP8 mice showed alterations in genes involved in protein acetylation homeostasis such as Sirt1 and Hdac6 and modulation of Hdac3 and Hdac5 gene expression by exercise. Global histone H3 acetylation levels were reduced in SAMP8 compared with SAMR1 mice and reached control levels in response to exercise. In sum, data presented here provide new candidate epigenetic markers for aging and neurodegeneration and suggest that exercise training may prevent or delay some epigenetic alterations associated with accelerated aging.
Analysis of interannual changes in northern vegetation activity observed in AVHRR data from 1981 to 1994

This paper reports on the analysis of Pathfinder AVHRR land (PAL) data set that spans the period July 1981 to September 1994. The time series of normalized difference vegetation index (NDVI) data for land areas north of 45/spl deg/ N assembled by correcting the PAL data with spectral methods confirms the northerly greening trend and extension of the photosynthetically active growing season. Analysis of the channel reflectance data indicates that the interannual changes in red and near-infrared reflectances are similar to seasonal changes in the spring time period when green leaf area increases and photosynthetic activity ramps up. Model calculations and theoretical analysis of the sensitivity of NDVI to background reflectance variations confirm the hypothesis that warming driven reductions in snow cover extent and earlier onset of greening are responsible for the observed changes in spectral reflectances over vegetated land areas.
Direct Antigen Presentation and Gap Junction Mediated Cross-Presentation during Apoptosis1

MHC class I molecules present peptides from endogenous proteins. Ags can also be presented when derived from extracellular sources in the form of apoptotic bodies. Cross-presentation of such Ags by dendritic cells is required for proper CTL responses. The fate of Ags in cells initiated for apoptosis is unclear as is the mechanism of apoptosis-derived Ag transfer into dendritic cells. Here we show that novel Ags can be generated by caspases and be presented by MHC class I molecules of apoptotic cells. Since gap junctions function until apoptotic cells remodel to form apoptotic bodies, transfer and cross-presentation of apoptotic peptides by neighboring and dendritic cells occurs. We thus define a novel phase in classical Ag presentation and cross-presentation by MHC class I molecules: presentation of Ags created by caspase activities in cells in apoptosis.
A Social Network Based Patching Scheme for Worm Containment in Cellular Networks

Recently, cellular phone networks have begun allowing third-party applications to run over certain open-API phone operating systems such as Windows Mobile, Iphone and Google’s Android platform. However, with this increased openness, the fear of rogue programs written to propagate from one phone to another becomes ever more real. This chapter proposes a counter-mechanism to contain the propagation of a mobile worm at the earliest stage by patching an optimal set of selected phones. The counter-mechanism continually extracts a social relationship graph between mobile phones via an analysis of the network traffic. As people are more likely to open and download content that they receive from friends, this social relationship graph is representative of the most likely propagation path of a mobile worm. The counter-mechanism partitions the social relationship graph via two different algorithms, balanced and clustered partitioning and selects an optimal set of phones to be patched first as those have the capability to infect the most number of other phones. The performance of these partitioning algorithms is compared against a benchmark random partitioning scheme. Through extensive trace-driven experiments using real IP packet traces from one of the largest cellular networks in the US, we demonstrate the efficacy of our proposed counter-mechanism in containing a mobile worm.
Pharmacokinetic changes of a new carbapenem, DA-1131, after intravenous administration to spontaneously hypertensive rats and deoxycorticosterone acetate-salt-induced hypertensive rats.

The pharmacokinetics of a new carbapenem, DA-1131, were compared after i.v. administration of the drug, 50 mg/kg, to spontaneously hypertensive rats (SHRs) at 16 weeks of age (an animal model for human primary hypertension) and at 6 weeks of age (corresponding to the early phase of the development of hypertension, at which time blood pressure remains within the normotensive range) and their respective age-matched control normotensive Kyoto-Wistar rats (KW rats), and deoxycorticosterone acetate-salt-induced hypertensive rats at 16 weeks of age (an animal model for human secondary hypertension) and their age-matched control Sprague-Dawley rats. The total area under the plasma concentration-time curve from time zero to time infinity (AUC) (4720 versus 7070 microg x min/ml) was significantly smaller, and the nonrenal clearance (CLNR) (5.37 versus 3.57 ml/min/kg) was significantly faster in 16-week-old SHRs than those in their control KW rats. Similar results were also obtained from 6-week-old SHRs in AUC (3800 versus 4680 microg x min/ml) and CLNR (7.73 versus 3.31 ml/min/kg). However, the values were reversed in 16-week-old deoxycorticosterone acetate-salt rats in AUC (5310 versus 3870 microg.min/ml) and CLNR (2.57 versus 4.90 ml/min/kg). The significantly faster CLNR of DA-1131 in both 6- and 16-week-old SHRs could be supported at least partly by the results of the in vitro metabolism with kidney homogenate and considerably greater total renal dehydropeptidase-I activity. The data above indicated that the significantly faster CLNR of DA-1131 in 16-week-old SHRs than that in their age-matched control KW rats was due to any hereditary characteristics of SHRs and was not due to the hypertensive state itself.
Practical application of digital fractional-order controller to temperature control

Prakticka aplikacia cislicoveho regulatora necelociselneho radu na riadenie teploty Prispevok sa zaobera praktickým navrhom a aplikaciou cislicoveho regulatora necelociselneho radu na riadenie teploty žiarica. V prispevku je ďalej uvedený matematický popis regulatorov a regulovaných sustav necelociselneho radu. Pre použitie cislicových regulatorov necelociselneho radu je uvedený prislusný algoritmus. Vlastnosti regulacneho obvodu s regulatorom necelociselneho radu su porovnane s regulacným obvodom s klasickým regulatorom celociselneho radu a dosiahnute výsledky su uvedene a diskutovane v zavere prispevku.
Portfolio Blending via Thompson Sampling

As a definitive investment guideline for institutions and individuals, Markowitz's modern portfolio theory is ubiquitous in financial industry. However, its noticeably poor out-of-sample performance due to the inaccurate estimation of parameters evokes unremitting efforts of investigating effective remedies. One common retrofit that blends portfolios from disparate investment perspectives has received growing attention. While even a naive portfolio blending strategy can be empirically successful, how to effectually and robustly blend portfolios to generate stable performance improvement remains less explored. In this paper, we present a novel online algorithm that leverages Thompson sampling into the sequential decision-making process for portfolio blending. By modeling blending coefficients as probabilities of choosing basis portfolios and utilizing Bayes decision rules to update the corresponding distribution functions, our algorithm sequentially determines the optimal coefficients to blend multiple portfolios that embody different criteria of investment and market views. Compared with competitive trading strategies across various benchmarks, our method shows superiority through standard evaluation metrics.
B2 bradykinin receptor immunoreactivity in rat brain

Bradykinin has long been known to exist in the central nervous system and has been hypothesized to mediate specific functions. Despite an increasing understanding of the functions of bradykinin, little is known about the cell types expressing the bradykinin receptor within the brain. The present investigation employed a monoclonal antibody directed against the 15–amino–acid portion of the C–terminal of the human bradykinin B2 receptor to establish the cellular distribution of bradykinin B2 receptor immunoreactivity in the rat brain. Bradykinin B2 receptor immunoreactivity was ubiquitously and selectively observed in neurons, including those within the olfactory bulb , cerebral cortex, hippocampus, basal forebrain, basal ganglia, thalamus, hypothalamus, cerebellum, and brainstem nuclei. Bradykinin B2 receptor immunoreactivity was also present in the circumventricular organs including choroid plexus, subfornical organ, median eminence, and area postrema. Double–labeling experiments colocalizing the bradykinin B2 receptor with the neuronal marker NeuN or the astrocytic marker glial fibrillary acidic protein revealed that virtually 100% of the bradykinin B2 receptor–immunoreactive positive cells were neurons. The widespread distribution of bradykinin B2 receptor immunoreactivity in neuronal compartments suggests a greater than previously appreciated role for this peptide in neuronal function. J. Comp. Neurol. 427:1–18, 2000. © 2000 Wiley‐Liss, Inc.
[Expression of high mobility group box-1 in the lung tissue and BALF of asthmatic mice and the influence of dexamethasone].

OBJECTIVE To investigate the expression of high mobility group box-1 (HMGB1) in the lung tissue and bronchoalveolar lavage fluid (BALF) of asthmatic mouse models and the influence of dexamethasone (DM). METHODS Eighteen female Balb/C mice were randomly divided PBS control group, OVA group and OVA/DM group, and asthmatic mouse models were established in the latter two groups. The airway responsiveness of the mice was assessed by whole-body plethysmography, and the cells in the BALF were counted and classified, with the supernatants of the BALF collected for detection of the level of HMGB1 by ELISA. The left lung of the mice was collected for HE staining, and the expression of HMGB1 in the right lung tissue was detected by Western blotting. RESULTS Asthmatic mouse models were successfully established. The level of HMGB1 in the BALF was significantly higher in OVA group than in the control group (6.31 ± 4.05 ng/ml vs 2.59 ± 0.73 ng/ml, P = 0.017), but no significant difference was found between OVA/DM group (3.39 ± 0.50 ng/ml) and OVA group (PP = 0.052). The expression of HMGB1 relative to tubulin was significantly higher in OVA group than in the control group (2.08 ± 0.87 vs 0.85 ± 0.30, P = 0.032), but similar between OVA/DM group (1.15 ± 0.48) and OVA group (PP = 0.133). CONCLUSION The expression of HMGB1 is obviously increased in the lung and BALF of asthmatic mice and DM produces no significant effect on HMGB1 expression, suggesting that HMGB1 may serve as a new therapeutic target for asthma treatment.
1,25-Dihydroxyvitamin D3 Promotes a Sustained LPS-Induced NF-κB-Dependent Expression of CD55 in Human Monocytic THP-1 Cells

The vitamin D3 system imposes immunosuppressive effects on monocytic cells, in part, by inhibiting NF-κB-dependent expression of proinflammatory mediators. CD55, a cell surface complement regulatory protein that promotes protective and anti-inflammatory properties, is reportedly an NF-κB target gene transiently induced in monocytic cells by the bacterial endotoxin LPS. CD55 is elevated on white cells in women experiencing preterm labor (a pathophysiology commonly associated with bacterial infection) and failure to maintain CD55 was associated with subsequent preterm delivery. We examined the influence of vitamin D3 signaling on LPS-induced expression of CD55 in human monocytic THP-1 cells using quantitative PCR, immunoblot, immunohistochemistry, and NF-κB activation pathway inhibitors. Non-NF-κB targets CD14 and CD11b, which modulate bacterial surveillance and eradication, respectively, were also examined. LPS produced a rapid transient 1.6-fold increase in CD55 mRNA. 1,25-D3 alone did not affect CD55 mRNA expression within the first 48 h. However, in 1,25-D3 pretreated cells, LPS produced a >4-fold immediate and sustained increase in CD55 mRNA and protein expression, which was blocked by NF-κB inhibitors. Our results unexpectedly suggest that vitamin D3 signaling may promote an anti-inflammatory response through an NF-κB-dependent increase in CD55 expression. As expected, LPS or 1,25-D3 alone led to sustained increases in CD14 and CD11b expression. In 1,25-D3 pretreated cells, LPS differentially regulated protein expression - CD14 (21-fold increase) and CD11b (a transient 2-fold decrease) - principally at the posttranscriptional level. The coordinated temporal expression of CD55, CD14 and CD11b would contribute to an anti-inflammatory response by providing protection against complement-mediated cell lysis during pathogen recognition and eradication. Overall, the vitamin D3 system may play a role coordinating an anti-inflammatory response pattern of the host complement immune system. This may be particularly important when considering the high rates of preterm births in blacks, a population that exhibits reduced circulating vitamin D3 levels.
Quantification of Epicardial Fat by Cardiac CT Imaging

The aim of this work is to introduce and design image processing methods for the quantitative analysis of epicardial fat by using cardiac CT imaging. Indeed, epicardial fat has recently been shown to correlate with cardiovascular disease, cardiovascular risk factors and metabolic syndrome. However, many concerns still remain about the methods for measuring epicardial fat, its regional distribution on the myocardium and the accuracy and reproducibility of the measurements. In this paper, a method is proposed for the analysis of single-frame 3D images obtained by the standard acquisition protocol used for coronary calcium scoring. In the design of the method, much attention has been payed to the minimization of user intervention and to reproducibility issues. In particular, the proposed method features a two step segmentation algorithm suitable for the analysis of epicardial fat. In the first step of the algorithm, an analysis of epicardial fat intensity distribution is carried out in order to define suitable thresholds for a first rough segmentation. In the second step, a variational formulation of level set methods - including a specially-designed region homogeneity energy based on Gaussian mixture models- is used to recover spatial coherence and smoothness of fat depots. Experimental results show that the introduced method may be efficiently used for the quantification of epicardial fat.
The Genetic Structure and History of Africans and African Americans

African Origins The modern human originated in Africa and subsequently spread across the globe. However, the genetic relationships among the diverse populations on the African continent have been unclear. Tishkoff et al. (p. 1035; see the cover, published online 30 April) provide a detailed genetic analysis of most major groups of African populations. The findings suggest that Africans represent 14 ancestral populations. Populations tend to be of mixed ancestry which documents historical migrations. The data mainly support but sometimes challenge proposed relationships between groups of self-identified ethnicity previously hypothesized on the basis of linguistic studies. The authors also examined populations of African Americans and individuals of mixed ancestry from Cape Town, documenting the variation and origins of admixture within these groups. A genetic study illuminates population history, as well as the relationships among and the origin of major language families. Africa is the source of all modern humans, but characterization of genetic variation and of relationships among populations across the continent has been enigmatic. We studied 121 African populations, four African American populations, and 60 non-African populations for patterns of variation at 1327 nuclear microsatellite and insertion/deletion markers. We identified 14 ancestral population clusters in Africa that correlate with self-described ethnicity and shared cultural and/or linguistic properties. We observed high levels of mixed ancestry in most populations, reflecting historical migration events across the continent. Our data also provide evidence for shared ancestry among geographically diverse hunter-gatherer populations (Khoesan speakers and Pygmies). The ancestry of African Americans is predominantly from Niger-Kordofanian (~71%), European (~13%), and other African (~8%) populations, although admixture levels varied considerably among individuals. This study helps tease apart the complex evolutionary history of Africans and African Americans, aiding both anthropological and genetic epidemiologic studies.
Brief report: complete deficiency of plasminogen-activator inhibitor type 1 due to a frame-shift mutation.

THE process of plasminogen activation is considered a critical component of diverse biologic systems in humans. This reaction, which is catalyzed in vivo by tissue-type and urokinase-type plasminogen activators, results in the conversion of plasminogen to plasmin. Plasmin has an essential role in maintaining vascular patency by converting fibrin to soluble fibrin-degradation products. In addition, plasminogen activators have been implicated in the regulation of embryogenesis, angiogenesis, ovulation, inflammation, and tumor metastasis,1 suggesting that the plasminogen-activation system is an important mediator of tissue remodeling and cell migration. The activity of plasminogen activators is precisely regulated by several mechanisms.1 Critical among these .