Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical application of sensitive troponin assays.

Despite the pervasive measurement of cardiac troponin for the diagnosis of myocardial infarction, the continued evolution of assays and guidelines for their application has created uncertainty among many practitioners regarding the use of cutoff values for clinical interpretation. As such, many clinicians may not welcome more sensitive assays for troponin. Nevertheless, professional societies have advocated for improved analytic performance of commercial assays, resulting in a current generation of widely used troponin assays that are more sensitive than their predecessors. In this issue of the Journal, important studies by Keller et al.1 and Reichlin et al.2 reveal the advantages and limitations .
Metabolic functions of AMPK: Aspects of structure and of natural mutations in the regulatory gamma subunits

AMP‐activated protein kinase, AMPK, is widely accepted as the master regulator of energy levels within the cell. Responding quickly to changing energy demands, AMPK works to restore levels of ATP during times of cellular stress by promoting ATP producing catabolic pathways and inhibiting ATP consuming anabolic ones. As a heterotrimeric protein complex, AMPK's subunits each act in unique and crucial ways to control AMPK function and its localization within the cell. Research in the last decade has identified and begun to characterize the impact of naturally occurring mutations in the gamma regulatory subunits. Mutations in the γ2 subunit have implications for cardiac function and disease, while the R225W mutation in the γ3 subunit have implications for skeletal muscle fuel metabolism and resistance to fatigue. Research focused on structure‐function aspects of AMPK regulatory subunits will lead to a better understanding of the roles of AMPK in health and disease. © 2010 IUBMB IUBMB Life, 62(10): 739–745, 2010
Preliminary Validation of Direct Detection of Foot-And-Mouth Disease Virus within Clinical Samples Using Reverse Transcription Loop-Mediated Isothermal Amplification Coupled with a Simple Lateral Flow Device for Detection

Rapid, field-based diagnostic assays are desirable tools for the control of foot-and-mouth disease (FMD). Current approaches involve either; 1) Detection of FMD virus (FMDV) with immuochromatographic antigen lateral flow devices (LFD), which have relatively low analytical sensitivity, or 2) portable RT-qPCR that has high analytical sensitivity but is expensive. Loop-mediated isothermal amplification (LAMP) may provide a platform upon which to develop field based assays without these drawbacks. The objective of this study was to modify an FMDV-specific reverse transcription–LAMP (RT-LAMP) assay to enable detection of dual-labelled LAMP products with an LFD, and to evaluate simple sample processing protocols without nucleic acid extraction. The limit of detection of this assay was demonstrated to be equivalent to that of a laboratory based real-time RT-qPCR assay and to have a 10,000 fold higher analytical sensitivity than the FMDV-specific antigen LFD currently used in the field. Importantly, this study demonstrated that FMDV RNA could be detected from epithelial suspensions without the need for prior RNA extraction, utilising a rudimentary heat source for amplification. Once optimised, this RT-LAMP-LFD protocol was able to detect multiple serotypes from field epithelial samples, in addition to detecting FMDV in the air surrounding infected cattle, pigs and sheep, including pre-clinical detection. This study describes the development and evaluation of an assay format, which may be used as a future basis for rapid and low cost detection of FMDV. In addition it provides providing “proof of concept” for the future use of LAMP assays to tackle other challenging diagnostic scenarios encompassing veterinary and human health.
Increase in IL-6 levels among major depressive disorder patients after a 6-week treatment with duloxetine 60 mg/day: a preliminary observation

Background: Immune modifications, including changes in interleukin (IL)-6 levels, have often been observed in major depressive disorder (MDD) during treatment with selective serotonin reuptake inhibitors (SSRIs) or the serotonin norepinephrine reuptake inhibitor (SNRI) venlafaxine. Nevertheless, no equivalent observation for the SNRI duloxetine has been made to date. Method: Sixteen patients diagnosed with MDD and an actual major depressive episode according to DSM-IV criteria and 16 healthy controls entered a 6-week trial with duloxetine 60 mg/day. All subjects (n = 32) were assessed using the Hamilton Depression Rating Scale (HAM-D), the Young Mania Rating Scale (YMRS), and were monitored for IL-6 levels both at baseline and at week 6. Blood samples for IL-6 levels were evaluated by ELISA. Results: After 6 weeks of treatment, the mean total scores for HAM-D declined both in the depressed and control groups, while IL-6 modification showed an opposite trend both in depressed (12.38 ± 19.80 to 19.73 ± 18.94 pg/mL) and control subjects (12.25 ± 21.12 to 17.63 ± 20.44 pg/mL), as did YMRS (ns), although none of the subjects switched to (hypo)mania. Of note, IL-6 levels increased significantly only in the responders subgroup (n = 9; P = 0.012). Conclusion: The small sample size and weak design of this study limit the validity of our results, which should be regarded as preliminary only. Nonetheless, the trend of increasing IL-6 levels observed in responder patients treated with duloxetine should prompt further controlled, extended studies with larger samples, with the specific aim of better assessing a putative differential role of norepinephrinergic antidepressant stimulation of serotonergic reuptake inhibition in determining modifications in IL-6 levels. Ideally, more accurate replication studies may contribute to further understanding of the complex interaction of mood, antidepressant response, and the immune system.
Effect of growth temperature on structural and electronic properties of ZnO thin films

The electronic and structural properties for RF magnetron sputtering deposited ZnO thin films grown on Si substrate was obtained by using X-ray diffraction (XRD) and reflection electron energy loss spectroscopy (REELS). XRD spectra show the intensity of the diffraction peak increases with increasing the growth temperature with (002) is strongest diffraction peak. The particle sizes for (002) are increase from 10.2 nm to 60.2 nm with increasing growth temperature from room temperature to 500oC, respectively. The band gap of ZnO thin films REELS spectra are 3.1±0.1 eV. The dominant peak from REELS at about 18 eV is ascribed to a bulk Plasmon excitation, which represents the collective oscillation of the valence electrons excited by the incident fast electrons.
H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination

H2O2 is known as a signal molecule in plant cells, but its role in the regulation of aqbscisic acid (ABA) and gibberellic acid (GA) metabolism and hormonal balance is not yet clear. In this study it was found that H2O2 affected the regulation of ABA catabolism and GA biosynthesis during seed imbibition and thus exerted control over seed dormancy and germination. As seen by quantitative RT-PCR (QRT-PCR), H2O2 up-regulated ABA catabolism genes (e.g. CYP707A genes), resulting in a decreased ABA content during imbibition. This action required the participation of nitric oxide (NO), another signal molecule. At the same time, H2O2 also up-regulated GA biosynthesis, as shown by QRT-PCR. When an ABA catabolism mutant, cyp707a2, and an overexpressing plant, CYP707A2-OE, were tested, ABA content was negatively correlated with GA biosynthesis. Exogenously applied GA was able to over-ride the inhibition of germination at low concentrations of ABA, but had no obvious effect when ABA concentrations were high. It is concluded that H2O2 mediates the up-regulation of ABA catabolism, probably through an NO signal, and also promotes GA biosynthesis. High concentrations of ABA inhibit GA biosynthesis but a balance of these two hormones can jointly control the dormancy and germination of Arabidopsis seeds.
Advances in the treatment of patients with gastric adenocarcinoma

Despite a decline in its incidence in the Western world, gastric cancer (GC) remains the fourth most frequent cancer diagnosis worldwide and is, after lung cancer, the second leading cause of death from a malignant disease globally. Based on the published literature, treatment guidelines and reports from international meetings, we here review the current treatment options for GC and discuss insights and perspectives from the latest clinical studies. The management of GC in the early stages of the disease is based on an optimal surgical resection of the primary tumor and the regional lymph nodes. However, less than one third of patients have a resectable disease at diagnosis and among those operated, more than half are not cured by surgery alone, due to a high rate of relapse. Thus, for the majority of patients, systemic cytotoxic therapy, and sometimes radiotherapy, is a treatment option both as an adjunct to surgery and in the palliative setting. Adjuvant chemotherapy offers only a marginal benefit and has not become a standard of care in the West. In North America, adjuvant chemoradiation is broadly used, shown to significantly improve overall survival, albeit with the cost of high toxicity. Furthermore, a recently reported study from the United Kingdom demonstrated a significant disease-free and survival benefit by the use of perioperative combination chemotherapy. Several chemotherapeutic agents have been tested as a palliative therapy in advanced GC including 5- fluorouracil (5-FU), oral pyrimidines, platinum derivatives, anthracyclines, taxanes and camptothecans. It is now accepted that chemotherapy is better than best supportive care only and that 5-FU based combinations are more effective than monotherapy. However, the response rates have generally been moderate and there is no consensus on the optimal combination of cytotoxic agents and the potential role of more recently developed “targeted therapies”.
Defining Relations: A General Incremental Approach with Spatial Temporal Case Studies

This paper aims to lay a foundation for a systematic study of mechanisms for construction of definitions within a formal theory, by investigating operators for incremental construction of definitions of new relations from an existing set of primitives and previously defined relations. To illustrate our method, we apply it to two of the best known relation sets studied in KRR: Allen's Interval Algebra and Region Connection Calculus. We also show that systematic exploration of definitional possibilities can yield interesting insights into relation sets that were originally defined in a more ad hoc way, and opens the possibility for discovering new vocabulary for extending or refining existing calculi or for developing completely new calculi.
Adapted Time-Optimal Trajectory Planning for Parallel Manipulators with Full Dynamic Modelling

This article provides a systematic insight into planning time-minimal trajectories for 6-dof parallel manipulators. Most of wide spread model simplifications, especially known from serial manipulators, are removed in order to give a general solution. All rigid-body forces, dry friction, viscous damping in passive and in actuated joints are considered. This yields a considerable escalation of the task’s complexity. Therefore, an adapted algorithm is presented, that ensures the computational efficiency of trajectory planning, even in cases of high complex dynamics.
Huntington's disease: Molecular basis of pathology and status of current therapeutic approaches

Huntington's disease (HD) is a frequent and incurable hereditary neurodegenerative disorder that impairs motor and cognitive functions. Mutations in huntingtin (HTT) protein, which is essential for neuronal development, lead to the development of HD. An increase in the number of CAG repeats within the HTT gene, which lead to an expansion of polyglutamine tract in the resulting mutated HTT protein, which is toxic, is the causative factor of HD. Although the molecular basis of HD is known, there is no known cure for this disease other than symptomatic relief treatment approaches. The toxicity of mutHTT appears to be more detrimental to striatal medium spiny neurons, which degenerate in this disease. Therapeutic strategies addressing a reduction in the mutHTT content at the transcriptional level using zinc finger proteins and at the translational level with RNA interference and antisense oligonucleotides or promoting the proteosomal degradation of mutHTT are being studied extensively in preclinical models and also to a limited extent in clinical trials. The post-translational modification of mutHTT is another possibility that is currently being investigated for drug development. In addition to the pharmacological approaches, several lines of evidence suggested the potential therapeutic use of stem cell therapy, in particular using the patient-derived induced pluripotent stem cells, to replace the lost striatal neurons. The multi-pronged clinical investigations currently underway may identify therapies and potentially improve the quality of life for the HD patients in future.
The Ahlswede-Han conjecture on channel with coded side information at the decoder

We study the Ahlswede-Han problem of single-user communication with partial state information available at the destination. For a class of channels, we show that the Wyner-Ziv compression of the channel state treating the receiver's channel output as side information is optimal. This result is more general than the modulo-sum channel studied by Aleksic et. al in 2009. Thus, for this more general class of channels, we prove the Ahlswede-Han conjecture.
On the Detection of Signaling DoS Attacks on 3G Wireless Networks

Third generation (3G) wireless networks based on the CDMA2000 and UMTS standards are now increasingly being deployed throughout the world. Because of their complex signaling and relatively limited bandwidth, these 3G networks are generally more vulnerable than their wireline counterparts, thus making them fertile ground for new attacks. In this paper, we identify and study a novel denial of service (DoS) attack, called signaling attack, that exploits the unique vulnerabilities of the signaling/control plane in 3G wireless networks. Using simulations driven by real traces, we are able to demonstrate the impact of a signaling attack. Specifically, we show how a well-timed low-volume signaling attack can potentially overload the control plane and detrimentally affect the key elements in a 3G wireless infrastructure. The low-volume nature of the signaling attack allows it to avoid detection by existing intrusion detection algorithms, which are often signature or volume-based. As a counter-measure, we present and evaluate an online early detection algorithm based on the statistical CUSUM method. Through the use of extensive trace-driven simulations, we demonstrate that the algorithm is robust and can identify an attack in its inception, before significant damage is done.
Distinct temporal requirements for Runx1 in hematopoietic progenitors and stem cells

The transcription factor Runx1 is essential for the formation of yolk sac-derived erythroid/myeloid progenitors (EMPs) and hematopoietic stem cells (HSCs) from hemogenic endothelium during embryogenesis. However, long-term repopulating HSCs (LT-HSCs) persist when Runx1 is conditionally deleted in fetal liver cells, demonstrating that the requirement for Runx1 changes over time. To define more precisely when Runx1 transitions from an essential factor to a homeostatic regulator of EMPs and HSCs, and whether that transition requires fetal liver colonization, we performed conditional, timed deletions of Runx1 between E7.5 and E13.5. We determined that Runx1 loss reduces the formation or function of EMPs up through E10.5. The Runx1 requirement in HSCs ends later, as deletion up to E11.5 eliminates HSCs. At E11.5, there is an abrupt transition to Runx1 independence in at least a subset of HSCs that does not require fetal liver colonization. The transition to Runx1 independence in EMPs is not mediated by other core binding factors (Runx2 and/or Runx3); however, deleting the common non-DNA-binding β subunit (CBFβ) severely compromises LT-HSC function. Hence, the requirements for Runx1 in EMP and HSC formation are temporally distinct, and LT-HSC function is highly reliant on continued core binding factor activity.
Multicolour Emission States from Charge Transfer between Carbon Dots and Surface Molecules

The emissive states of carbon dots have been tuned by controlling the charge transfer process. The carbon dots couple with molecules, which are made of a benzene ring and different heteroatom substituents, through amino-carboxylic bonds that are generally identified as charge transfer promoters at the interface. New ways of radiative recombination are created due to the transfer of photo-excited electrons from carbon dots to the lowest unoccupied molecular orbital (LUMO) of the grafted molecules. By variation of the molecular orbital energy levels via heteroatom substituents in the benzene ring, the different optical properties and emission colors of the carbon dots were presented. This work opens up new opportunities for the application of carbon dots since different heteroatom substituents could lead to many possibilities for conjugation with drugs and biomolecules.
In vivo aging of gutta-percha dental cone

Gutta-percha cone is the most widely used material for root canal filling. The in vivo aging of this cone focus on the degradation of its main organic component, trans-1,4-polyisoprene, was studied. Aged cones (25 samples) from 2 to 30 years of root canal filling were extracted from different patients in the occasion of retreatment by mechanical way. The information about the aging time was given by the patients. Gel-permeation chromatography (GPC) and infrared spectroscopy (FTIR) were the analytical techniques used. Polyisoprene degrades with time of aging, but in a slow process. Decrease in polymer molar mass from 5.7 × 105 to 1.7 × 105 g/mol was observed in polyisoprene from cone after 30 years of root canal filling and inside a noninfected tooth. In tooth with caries and periodontal infection, the decrease in molar mass is higher (4.6 × 104 g/mol in cone with 10 years of aging). The production of carbonyl and hydroxyl groups in the aged material indicates that the process is oxidative, even in closed teeth. In these cases, the oxygen could be provided from tissue fluid. The degradation mechanism is complex and depends on many factors, besides time of root canal filling. The dental problem caused by the aging could be the production and migration of cytotoxic substances to periodontal ligament and the reduction on the canal sealing property due to the polymer weight loss. Both of them could contribute to the root canal treatment failure. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100:4082–4088, 2006
Quasi-Stationary Distributions and the Continuous-State Branching Process Conditioned to Be Never Extinct

We consider continuous-state branching (CB) processes which become extinct (i.e., hit 0) with positive probability. We characterize all the quasi-stationary distributions (QSD) for the CB-process as a stochastically monotone family indexed by a real number. We prove that the minimal element of this family is the so-called Yaglom quasi-stationary distribution, that is, the limit of one-dimensional marginals conditioned on being nonzero. Next, we consider the branching process conditioned on not being extinct in the distant future, or $Q$-process, defined by means of Doob $h$-transforms. We show that the $Q$-process is distributed as the initial CB-process with independent immigration, and that under the $L\log L$ condition, it has a limiting law which is the size-biased Yaglom distribution (of the CB-process). More generally, we prove that for a wide class of nonnegative Markov processes absorbed at 0 with probability 1, the Yaglom distribution is always stochastically dominated by the stationary probability of the $Q$-process, assuming that both exist. Finally, in the diffusion case and in the stable case, the $Q$-process solves a SDE with a drift term that can be seen as the instantaneous immigration.
Large Scale CVR Prediction through Dynamic Transfer Learning of Global and Local Features

This paper presents a combination of strategies for conversion rate (CVR) prediction deployed at the Yahoo! demand side platform (DSP) Brightroll, targeting at modeling extremely high dimensional, sparse data with limited human intervention. We propose a novel probabilistic generative model by tightly integrating components of natural language processing, dynamic transfer learning and scalable prediction, named Dynamic Transfer Learning with Reinforced W ord M odeling (a.k.a. Trans-RWM ) to predict user conversion rates. Our model is based on assumptions that: on a higher level, information can be transferable between related campaigns; on a lower level, users who searched similar contents or browsed similar pages would have a higher probability of sharing similar latent purchase interests. Novelties of this framework include (i) A novel natural language modeling specifically tailored for semantic inputs of CVR prediction; (ii) A Bayesian transfer learning model to dynamically transfer the knowledge from source to the future target ; (iii) An automatic new updating rule with adaptive regularization using Stochastic Gradient Monte Carlo to support the efficient updating of Trans-RWM in high-dimensional and sparse data. We demonstrate that on Brightroll our framework can effectively discriminate extremely rare events in terms of their conversion propensity.
UvA-DARE (Digital Academic Repository) Approach, avoidance, and affect A meta-analysis of approach-avoidance tendencies in manual reaction time tasks Approach, avoidance, and affect: a meta-analysis of approach-avoidance tendencies in manual reaction time tasks

Approach action tendencies toward positive stimuli and avoidance tendencies from negative stimuli are widely seen to foster survival. Many studies have shown that approach and avoidance arm movements are facilitated by positive and negative affect, respectively. There is considerable debate whether positively and negatively valenced stimuli prime approach and avoidance movements directly (i.e., immediate, unintentional, implicit, automatic, and stimulus-based), or indirectly (i.e., after conscious or non-conscious interpretation of the situation). The direction and size of these effects were often found to depend on the instructions referring to the stimulus object or the self, and on explicit vs. implicit stimulus evaluation. We present a meta-analysis of 29 studies included for their use of strongly positive and negative stimuli, with 81 effect sizes derived solely from the means and standard deviations (combined N = 1538), to examine the automaticity of the link between affective information processing and approach and avoidance, and to test whether it depends on instruction, type of approach-avoidance task, and stimulus type. Results show a signiﬁcant small to medium-sized effect after correction for publication bias. The strongest arguments for an indirect link between affect and approach-avoidance were the absence of evidence for an effect with implicit evaluation, and the opposite directions of the effect with self and object-related interpretations. The link appears to be inﬂuenced by conscious or non-conscious intentions to deal with affective stimuli.