Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of cell cycle events in asymmetrically dividing cells: functions required for DNA initiation and chain elongation in Caulobacter crescentus

To study the regulation of cell cycle events after asymmetric cell division in Caulobacter crescentus, we have identified functions that are required for DNA synthesis in the stalked cell produced at division and in the new stalked cell that develops from the swarmer cell 60 min after division. The initiation of DNA synthesis in the two progeny cells is dependent upon at least two common functions. One of these is a requirement for protein synthesis and the other is a gene product identified in a temperature-sensitive cell cycle mutant. DNA chain elongation requires a third common function. The characteristic pattern of DNA synthesis in C. crescentus appears to be controlled in part by the expression of these functions in the two stalked cells at different times after cell division. The age distribution for Caulobacter cells in an exponential population has been calculated (Appendix by Robert Tax) and used to analyze some of the results.
The effect of intrauterine human chorionic gonadotropin injection before embryo transfer on the implantation and pregnancy rate in infertile patients: A randomized clinical trial

Background: Implantation is one of the essential steps for the success of assisted reproductive techniques (ART). Their success depends on three main factors: embryo quality, endometrial receptivity (ER), and synchrony between embryo and endometrium. There are various factors that regulate the complex process of implantation. In this regard, one may refer to human chorionic gonadotropin (hCG) as the most important factor. Objective: This study aims to investigate the effect of intrauterine hCG injection before embryo transfer (ET) on pregnancy outcome in infertile couples. Materials and Methods: A total of 159 patients undergone In vitro Fertilization/ Intracytoplasmic Sperm Injection (IVF/ICSI) with an antagonist protocol were evaluated. Patients were divided into three groups (n=53). Group 1 received 500 IU of hCG, group 2 received 1000 IU of hCG intrauterine injection before ET, and the control group underwent ET without hCG preceding intrauterine injection. Results: There was no significant difference among the groups. The implantation rates were 18.86%, 13.52%, and 14.37%, chemical pregnancy rates were 34%, 32.1%, and 35.3%, and clinical pregnancy rates were 34%, 32.1%, and 31.4% respectively. Conclusion: The pregnancy outcome in IVF/ICSI /ET cycles cannot be improved through hCG intrauterine injections before ET.
Scattering on stratified media: the microlocal properties of the scattering matrix and recovering asymptotics of perturbations

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Isolation and characterization of human primary enterocytes from small intestine using a novel method

Abstract Cell culture studies of enterocytes are important in many fields. However, there are difficulties in obtaining cell lines from adult human intestine, such as microbial contamination of cultures from the tissue samples, short life span of enterocytes, overgrowth of mesenchymal cells, etc. Various model used to obtain adult intestinal cell lines are very complex requiring use of feeder layer or gel matrices. The aim of this study was to establish a novel method for the simple and reproducible isolation of human enterocytes. Enterocytes were isolated from SI samples (n = 5) obtained from cadaveric donors using a mechanical procedure, and separation with immunomagnetic beads coated with anti-EpCAM antibodies. Light and electron microscopy, flow cytometry and immunocytochemistry techniques were used to characterize the isolated cells. Immunohistochemical staining of normal SB biopsies confirmed that the cell cultures maintained an in vivo phenotype as reflected in cytokeratin expression CK18, CK20 and expression of intestine-specific markers such as sucrase isomaltase and maltase glucoamylase. Furthermore, the cells strongly expressed TLR-5, 6, 7, 8 and 10 and several molecules such as CD40, CD86, CD44, ICAM-1 and HLA-DR which are important in triggering cell-mediated immune responses. This novel technique provides a unique in vitro system to study the biology of enterocytes in normal conditions as well as to study inflammatory processes in various small bowel disorders.
Healing the incest wound : adult survivors in therapy

Occasionally a book for psychotherapists instantly becomes a classic. Not only do clinicians recognize the excellence and importance of the book, but their clients respond enthusiastically to the accurate portrayal of their experiences and the model for healing. With the publication of Healing the Incest Wound, such a classic was born. Incest is not a rare aberrant happening, but a common childhood experience for a substantial minority of children. Since incest is generally hidden and denied, the victims are left to cope with their reactions in an atmosphere that contradicts their reality. Yet all incest is not the same; for instance, one-time fondling by an uncle has different effects from rapes repeated over many years by a stepfather. The book describes these variations and the symptoms, short-term aftereffects, and long-term secondary elaborations of incest from four theoretical perspectives: traumatic stress, developmental, feminist, and loss. The author not only comprehensively discusses the salient issues of incest therapy but also illustrates these with numerous case studies, showing how incest survivors can heal and build a core of self-respect and dignity.
SEMI-SUPERVISED OBJECT BASED DIGITAL MEASURABLE IMAGE SEQUENCE SEGMENTATION FOR MMS

Mobile mapping system (MMS) is the trend of state of art mapping technique and digital measurable image sequence is the main data type of Mobile mapping system. As one of the most important pre-processing for mobile mapping system, it is necessary to segment digital measurable image sequences in to manageable and meaningful objects such as guideposts. This paper proposed a novel semi-supervised object based image sequence segmentation method. Firstly, the object of interest such as guideposts or mailbox is specified by the user in a key frame. Secondly, color information is employed to find the candidate region of the object of interest in the new frame. Then local invariant features such as SIFT are employed to describe the object of interest. A novel matching is done in the following frames to segment the region of the object of interest frame by frame. The object template is revised as the matching going on. The segmentation and modelling method we developed is applied to different digital measurable image sequences. Experimental results demonstrate the robustness and effectiveness of our method. * Correspondence Author: Cheng WANG, Department of Computer Science, School of Information Science and Tech., Xiamen University, Xiamen City, Fujian Province, China, 361005 chwang_nudt@263.net
The selectivity of responses to red‐green colour and achromatic contrast in the human visual cortex: an fMRI adaptation study

There is controversy as to how responses to colour in the human brain are organized within the visual pathways. A key issue is whether there are modular pathways that respond selectively to colour or whether there are common neural substrates for both colour and achromatic (Ach) contrast. We used functional magnetic resonance imaging (fMRI) adaptation to investigate the responses of early and extrastriate visual areas to colour and Ach contrast. High‐contrast red–green (RG) and Ach sinewave rings (0.5 cycles/degree, 2 Hz) were used as both adapting stimuli and test stimuli in a block design. We found robust adaptation to RG or Ach contrast in all visual areas. Cross‐adaptation between RG and Ach contrast occurred in all areas indicating the presence of integrated, colour and Ach responses. Notably, we revealed contrasting trends for the two test stimuli. For the RG test, unselective processing (robust adaptation to both RG and Ach contrast) was most evident in the early visual areas (V1 and V2), but selective responses, revealed as greater adaptation between the same stimuli than cross‐adaptation between different stimuli, emerged in the ventral cortex, in V4 and VO in particular. For the Ach test, unselective responses were again most evident in early visual areas but Ach selectivity emerged in the dorsal cortex (V3a and hMT+). Our findings support a strong presence of integrated mechanisms for colour and Ach contrast across the visual hierarchy, with a progression towards selective processing in extrastriate visual areas.
Shift-Reduce Dependency DAG Parsing

Most data-driven dependency parsing approaches assume that sentence structure is represented as trees. Although trees have several desirable properties from both computational and linguistic perspectives, the structure of linguistic phenomena that goes beyond shallow syntax often cannot be fully captured by tree representations. We present a parsing approach that is nearly as simple as current data-driven transition-based dependency parsing frameworks, but outputs directed acyclic graphs (DAGs). We demonstrate the benefits of DAG parsing in two experiments where its advantages over dependency tree parsing can be clearly observed: predicate-argument analysis of English and syntactic analysis of Danish with a representation that includes long-distance dependencies and anaphoric reference links.
Beyond health and well-being: transformation, memory and the virtual in older people’s music and dance

Research exploring older people and the participatory arts has tended to focus on notions of biomedical impact, often coupled with appeals to evasive notions of “well-being. ” Rather than suggesting such approaches are invalid, this article proposes the need for their extension and proposes an alternative, critical approach to analysing older people’s experience of arts participation. Based on ethnographic participant observation and intensive consultation with a cohort of older people engaged in a programme of creative music and dance, we explore the complex processes and possibilities of transformation that the participatory arts can initiate, examining how performance can create intriguing linkages between past, present and future experiences. Taking a phenomenological approach to the study of memory, recollection, reminiscence and future anticipation, we discuss how arts participation can “actualise” potential memories in older participants, examining how and why this kind of expressive activity animates the idea of “virtual” selves (after Bergson).
Constitutively Active Protein Kinase A Disrupts Motility and Chemotaxis in Dictyostelium discoideum

ABSTRACT The deletion of the gene for the regulatory subunit of protein kinase A (PKA) results in constitutively active PKA in the pkaR mutant. To investigate the role of PKA in the basic motile behavior and chemotaxis of Dictyostelium discoideum, pkaR mutant cells were subjected to computer-assisted two- and three-dimensional motion analysis. pkaR mutant cells crawled at only half the speed of wild-type cells in buffer, chemotaxed in spatial gradients of cyclic AMP (cAMP) but with reduced efficiency, were incapable of suppressing lateral pseudopods in the front of temporal waves of cAMP, a requirement for natural chemotaxis, did not exhibit the normal velocity surge in response to the front of a wave, and were incapable of chemotaxing toward an aggregation center in natural waves generated by wild-type cells that made up the majority of cells in mixed cultures. Many of the behavioral defects appeared to be the result of the constitutively ovoid shape of the pkaR mutant cells, which forced the dominant pseudopod off the substratum and to the top of the cell body. The behavioral abnormalities that pkaR mutant cells shared with regA mutant cells are discussed by considering the pathway ERK2 —| RegA —| [cAMP] → PKA, which emanates from the front of a wave. The results demonstrate that cells must suppress PKA activity in order to elongate along a substratum, suppress lateral-pseudopod formation, and crawl and chemotax efficiently. The results also implicate PKA activation in dismantling cell polarity at the peak and in the back of a natural cAMP wave.
Exposures and asthma outcomes using two different job exposure matrices in a general population study in northern Europe.

OBJECTIVE We have recently published a study on new-onset asthma in a large population in northern Europe using a modified job exposure matrix (N-JEM) to better reflect exposure assignment in these countries. The aim of this paper was to investigate how the N-JEM differs in exposure assignment and asthma risks from an already established JEM. METHOD The study comprised 6253 men and 7031 women from northern Europe, born 1945-1973, who had answered both a screening (1989-1992) and a follow-up questionnaire (1999-2001). During the study period (1980-2000), there were 136 men and 293 women with new-onset asthma. Hazard ratios of new-onset asthma were calculated for both JEMs using Cox regression models. The analyses were made separately for men and women and were also stratified for atopy. Cohen's kappa (κ) was used to show agreements in exposure assignment (yes/no) between the JEMs. Population attributable risks (PARs) were calculated as well. RESULTS The agreement in exposure assignment between the JEMs was substantial for the group 'any exposure' to asthma agents (κ = 0.78). The agreement between comparable exposure groups in the JEMs varied from κ = 1.00 (pharmaceutical product antigens, textile dust, cleaning agents) to κ = 0.27 (low molecular weight agents). Significant increased asthma risks were seen for men exposed to isocyanates and accidental peak exposure with both JEMs. With the N-JEM, increased asthma risks were seen for men exposed to plant-associated antigens (all and non-atopic), epoxy compounds (all and non-atopic), and acrylates (non-atopic). With the other JEM, increased asthma risks were seen in men and women exposed to 'possible exposure to irritant gases or fumes' (all and non-atopic), a group classified as having low asthma risk. Men and women exposed to cleaning agents also showed significant asthma risks with both JEMs. PAR with the N-JEM was 14.3% for men and 6.6% for women, compared with 12.9% and 8.3% with the other JEM. CONCLUSIONS Acrylates, epoxy compounds, and isocyanates are three exposure groups in the modified asthma JEM that might better reflect exposure situations in northern Europe than the already established JEM. Exposure to 'possible exposure to irritant gases or fumes', a low asthma risk group in the established JEM, seems to be a group with high asthma risk in northern Europe. It is important to continuously update JEMs, which are based only on occupational titles, in order to find new risk groups and to better reflect changes in work exposures when old risks disappear and new emerge.
Topical Immunomodulation in Dermatology: Potential of Toll‐like Receptor Agonists

BACKGROUND Topical immunomodulators include both immunostimulatory and immunosuppressive agents. If successful, topical immunotherapy may represent an important improvement in the therapy of inflammatory dermatoses, viral infections, and cancers of the skin and genital mucosa. Topical immunotherapy using obligate contact sensitizers such as diphencyprone or dinitrochlorobenzene has been used against viral (e.g., common warts) and autoimmune diseases (e.g., alopecia areata). RESULTS Newer agents such imidazoquinolines (imiquimod and resiquimod) act by cytokine secretion from monocytes/macrophages (interferon-α, interleukin-12, tumor-necrosis factor-α). The locally generated immune milieu leads to a Th1-dominance and cell-mediated immunity that have been clinically used to treat viral infections such as human papillomavirus, herpes simplex virus, and mollusca. Although these agents improve antigen presentation by dendritic cells, they also act on B cells leading to the synthesis of antibodies such as IgG2a. We have also introduced this treatment against cancerous lesions including initial squamous cell and basal cell carcinoma in immunocompetent and immunosuppressed patients. We provide examples of successful treatment of squamous cell cancer using topical imiquimod. CONCLUSION The available and additional Toll-like receptor agonists will help to improve the specific dermatologic therapy. Topical immunotherapy with both immunostimulatory and immunosuppressive agents bears potential for effective and patient friendly treatment of inflammatory, infectious, and cancerous skin diseases. Long-term evaluation will define the tolerability and safety profile of these novel topical agents.
Exploring the intentionality of design in the graphic narrative of one middle-years student

The classroom-based research featured in this article explores how the development of student understanding of elements of visual art and design affected their comprehension, interpretation and analysis of multimodal texts, as well as the subsequent production of student-generated texts created in the medium of comics. This article presents the case study of Jarvinia, one of the grade 7 research participants, and focuses on her semiotic decision making when she designed a graphic narrative for the culminating activity of the research. Multi-literacies, multimodality, social semiotics and sociocultural theory framed the study as well as the descriptive analysis and discussion of the intentionality of Jarvinia’s meaning-making. A colour photocopy of Jarvinia’s graphic narrative and two interview transcripts constituted the sources of data that were examined to explore her text-making as a process of design. The findings from the descriptive case study reveal the intentionality of Jarvinia’s semiotic work when working with the medium of comics, and demonstrate how learning a metalanguage to talk about elements of visual art and design, and conventions of the medium of comics can inform more critical descriptions, interpretations and analysis of texts.
Joint bidding and geographical load balancing for datacenters: Is uncertainty a blessing or a curse?

We consider the scenario where a cloud service provider (CSP) operates multiple geo-distributed datacenters to provide Internet-scale service. Our objective is to minimize the total electricity and bandwidth cost by jointly optimizing electricity procurement from wholesale markets and geographical load balancing (GLB), i.e., dynamically routing workloads to locations with cheaper electricity. Under the ideal setting where exact values of market prices and workloads are given, this problem reduces to a simple LP and is easy to solve. However, under the realistic setting where only distributions of these variables are available, the problem unfolds into a non-convex infinite-dimensional one and is challenging to solve. Our main contribution is to develop an algorithm that is proven to solve the challenging problem optimally and efficiently, by exploring the full design space of strategic bidding. Trace-driven evaluations corroborate our theoretical results, demonstrate fast convergence of our algorithm, and show that it can reduce the cost for the CSP by up to 20% as compared to baseline alternatives. Our study highlights the intriguing role of uncertainty. While variability in workloads deteriorates the cost-saving performance of joint electricity procurement and GLB, counter-intuitively, variability in market prices can be exploited to achieve a cost reduction even larger than the setting without price variability.
The protective effects of IgM-enriched immunoglobulin and erythropoietin on the lung and small intestine tissues of rats with induced sepsis: Biochemical and histopathological evaluation

Abstract Context: Sepsis continues to be a significant problem for critical care patients. Objective: To evaluate the protective effects of IgM-enriched immunoglobulin and erythropoietin on pulmonary and small intestine tissues in a rat model of intra-abdominal sepsis induced via the cecal ligation and puncture (CLP) method. Materials and methods: Male Sprague–Dawley rats were used. Control group (n = 6): surgical procedure was not performed. Laparotomy was only performed in the sham group (n = 6) and CLP was only performed in the sepsis (CLP) group (n = 30). After erythropoietin (2000 U/kg, intraperitoneal) was given in the sepsis + erythropoietin (CLP + EPO) group (n = 30), IgM-enriched immunoglobulin (600 mg/kg, intraperitoneal) was given in the sepsis + pentaglobin (CLP + PEN) group (n = 30), CLP was created. Intracardiac blood samples were collected for biochemical analysis; lung and small intestine tissue samples were removed for histopathological evaluation. Results: Plasma TNF-α levels (pg/ml) were similar among CLP, CLP + EPO, and CLP + PEN groups (204.0 ± 52.4, 198.5 ± 17.3, and 214.6 ± 93.6, respectively). The CLP group had higher plasma IL-1β levels (pg/ml) compared with CLP + EPO and CLP + PEN groups (325.1 ± 134.1, 164.3 ± 25.6, and 186.3 ± 26.0, respectively) (p < 0.05). Rats in CLP + EPO and CLP + PEN groups had abolished histopathologic appearance of lung and small intestine tissues compared with rats in the CLP group. Discussion and conclusion: Our findings support the use of EPO and IgM-enriched immunoglobulin in the prevention of lung and small intestine injuries associated with sepsis.
Comparison of Predicted Amino Acid Sequences of Measles Virus Strains in the Edmonston Vaccine Lineage

ABSTRACT Protein-encoding nucleotide sequences of the N, P, M, F, H, and L genes were determined for a low-passage isolate of the Edmonston wild-type (wt) measles virus and five Edmonston-derived vaccine virus strains, including AIK-C, Moraten, Schwarz, Rubeovax, and Zagreb. Comparative analysis demonstrated a high degree of nucleotide sequence homology; vaccine viruses differed at most by 0.3% from the Edmonston wt strain. Deduced amino acid sequences predicted substitutions in all viral polypetides. Eight amino acid coding changes were common to all vaccine viruses; an additional two were conserved in all vaccine strains except Zagreb. Comparisons made between vaccine strains indicated that commercial vaccine lots of Moraten and Schwarz had identical coding regions and were closely related to Rubeovax, while AIK-C and Zagreb diverged from the Edmonston wt along slightly different paths. These comparisons also revealed amino acid coding substitutions in Moraten and Schwarz that were absent from the closely related reactogenic Rubeovax strain. All of the vaccine viruses contained amino acid coding changes in the core components of the virus-encoded transcription and replication apparatus. This observation, combined with identification of noncoding region nucleotide changes in potential cis-acting sequences of the vaccine strains (C. L. Parks, R. A. Lerch, P. Walpita, H.-P. Wang, M. S. Sidhu, and S. A. Udem, J. Virol. 75:921–933, 2001), suggest that modulation of transcription and replication plays an important role in attenuation.