Miracle-dz/UniFAR-FTUs huggingface dataset:
γ-Aminobutyric acid immunoreactivity in multiple cell types of the developing rabbit retina

Abstract We have previously demonstrated that the neonatal rabbit retina contains a larger complement of cells that accumulate [3H]-GABA than does the adult. In order for these neurons to be classified as GABAergic, they must also contain endogenous GABA. We now report that these same neonatal cell populations are also immunoreactive to GABA antisera. In frozen sections from rabbit retina, treated with GABA antisera, immunoreactive processes in both synaptic layers were observed at postnatal day 1. The appearance of immunofluorescent fibers precedes that of photoreceptor and bipolar cell terminals in the outer plexiform layer and is diminished by postnatal day 5. Also noted, was a 50% decrease in the density of GABA-immunoreactive cell bodies in the inner nuclear and ganglion cell layers, accompanied by an increase in cell volume and a shift toward a more spherical cell shape of the remaining cells. At postnatal day 1 and 3, we also observed immunoreactive cells having the characteristic morphology of interplexiform cells. This cell type sends branches to both the outer and inner plexiform layers, thus a morphological basis for communication between the two developing plexiform layers is present as early as postnatal day 1. Thus, retinas from neonatal rabbits have a larger complement of cells that stain for endogenous GABA than does the adult. These results coupled with our previous studies suggest that GABAergic properties are expressed by a larger number of cell types in the neonate than in the adult and are consistent with the general hypothesis that GABA functions as a trophic agent during development.
Day Care Attendance in the First Year of Life and Illnesses of the Upper and Lower Respiratory Tract in Children With a Familial History of Atopy

Objective. To examine the relationship between day care attendance and illnesses of the upper and lower respiratory tract in the first year of life. Study Design. Prospective birth cohort study. Methods. Children (N = 498) who had at least 1 parent with a history of allergy or asthma were enrolled at birth and followed prospectively for the first year of life. A home visit at 2 to 3 months of age and bimonthly telephone questionnaires were used to obtain information on day care arrangements, home characteristics, respiratory symptoms, and physician-diagnosed illnesses of the upper and lower respiratory tract. Results. Day care attendance in the first year of life was associated with two or more doctor-diagnosed ear infections (OR: 2.4; 95% CI: 1.7–3.6), three or more parental reports of runny or stuffed nose (OR: 3.2; 95% CI: 1.9–5.5), a doctor's diagnosis of sinusitis (OR: 2.2; 95% CI: 1.1–4.2), and doctor-diagnosed lower respiratory illnesses (croup, bronchitis, bronchiolitis, and pneumonia; OR: 1.6; 95% CI: 1.0–2.4). For children attending day care, exposure to pets in day care, the presence of a rug or carpet in the area where the child slept in day care, and a nonresidential setting for day care all were independent predictors of two or more doctor-diagnosed ear infections. Conclusions. The results suggest that day care increases the risk of illnesses of the upper and lower respiratory tract in the first year of life for children with a familial history of atopy. Specific environmental exposures within day care, such as the presence of pets or having a rug or carpet in the area where children sleep, may increase the risk of recurrent ear infections in the first year of life among children with familial history of atopy who attend day care.
Dynamic Network Surgery for Efficient DNNs

Deep learning has become a ubiquitous technology to improve machine intelligence. However, most of the existing deep models are structurally very complex, making them difficult to be deployed on the mobile platforms with limited computational power. In this paper, we propose a novel network compression method called dynamic network surgery, which can remarkably reduce the network complexity by making on-the-fly connection pruning. Unlike the previous methods which accomplish this task in a greedy way, we properly incorporate connection splicing into the whole process to avoid incorrect pruning and make it as a continual network maintenance. The effectiveness of our method is proved with experiments. Without any accuracy loss, our method can efficiently compress the number of parameters in LeNet-5 and AlexNet by a factor of $\bm{108}\times$ and $\bm{17.7}\times$ respectively, proving that it outperforms the recent pruning method by considerable margins. Code and some models are available at this https URL.
Prevalence of Human Metapneumovirus and Influenza Virus Infections Among Japanese Children During Two Successive Winters

Background: Human metapneumoviruses (hMPVs) are recognized as a leading cause of respiratory infections in young infants in many countries. The objective of this study was to identify links between hMPV and influenza virus infections among children with influenza-like illness. Method: This study was conducted in 2 influenza seasons (2002–2003 and 2003–2004) at 2 pediatric outpatient clinics in Niigata city, Japan. Nasopharyngeal swabs or aspirates were collected from influenza-like illness patients, and hMPV and influenza were detected by reverse transcription-polymerase chain reaction (RT-PCR). A nucleotide sequence of 352 nucleotides segment of the F gene was performed. Results: A total of 765 influenza viruses and 84 hMPV were identified from 1498 nasopharyngeal swabs or aspirates by virus isolation and RT-PCR, respectively. hMPV-positive rates in patients with influenza-like illness were 5.7 and 5.2% in the 2002–2003 and 2003–2004 seasons, respectively. Epidemic curves of influenza and hMPV patients showed similar patterns with peaks in February in 2 influenza seasons. hMPV infections occurred frequently in infants and school children. Approximately 46% of hMPV patients were coinfected with influenza A viruses, but those coinfected cases were not clinically distinct from the others. No coinfection with influenza B viruses was found. Phylogenetic analysis of the hMPV fusion gene sequences revealed that 2 distinct hMPV cocirculated and that completely identical strains in subgroup A were observed over 2 years. Conclusions: HMPV plays an important pathogenic role in patients with influenza-like illness in winter seasons, often in coinfections with influenza A viruses.
Commentary on comparison of MODIS snow cover and albedo products with ground observations over the mountainous terrain of Turkey

Papers published in Hydrology and Earth System Sciences Discussions are under open-access review for the journal Hydrology and Earth System Sciences Abstract The MODerate-resolution Imaging Spectroradiometer (MODIS) snow cover product was evaluated by Parajka and Bï osch (2006) over the territory of Austria. The spatial and temporal variability of the MODIS snow product classes are analyzed, the accuracy of the MODIS snow product against numerous in situ snow depth data are examined 5 and the main factors that may influence the MODIS classification accuracy are identified in their studies. The authors of this paper would like to provide more discussion to the scientific community on the " Validation of MODIS snow cover images " when similar methodology is applied to mountainous regions covered with abundant snow but with limited number of ground survey and automated stations. 10 Daily snow cover maps obtained from MODIS images are compared with ground observations in mountainous terrain of Turkey for the winter season of 2002–2003 and 2003–2004 during the accumulation and ablation periods of snow. Snow depth and density values are recorded to determine snow water equivalent values at 19 points in and around the study area in Turkey. Comparison of snow maps with in situ data 15 show good agreement with overall accuracies in between 62 to 82 percent considering a 2-day shift during cloudy days. Studies show that the snow cover extent can be used for forecasting of runoff hydrographs resulting mostly from snowmelt for a mountainous basin in Turkey. MODIS-Terra snow albedo products are also compared with ground based measure-20 ments over the ablation stage of 2004 using the automated weather operating stations (AWOS) records at fixed locations as well as from the temporally assessed measuring sites during the passage of the satellite. Temporarily assessed 20 ground measurement sites are randomly distributed around one of the AWOS stations and both MODIS and ground data were aggregated in GIS for analysis. Reduction in albedo is noticed 25 as snow depth decreased and SWE values increased.
Phenotypic and Genetic Characterization of a NovelBorrelia burgdorferi Sensu Lato Isolate from a Patient with Lyme Borreliosis

ABSTRACT Borrelia burgdorferi sensu lato A14S was cultured from a skin biopsy specimen of a patient with erythema migrans in The Netherlands. This isolate had a unique DNA fingerprint pattern compared to 135 other B. burgdorferi sensu lato isolates. In this study, the isolate A14S was further characterized by protein analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reactivity with various monoclonal antibodies. In addition, the 16S rRNA, ospA, and ospC genes, as well as the 5S-23S rRNA intergenic spacer DNA, were amplified by PCR, cloned, and sequenced. SDS-PAGE protein profiles and phylogenetic analysis based on all of the analyzed genes confirmed that B. burgdorferi sensu lato A14S was phenotypically and genetically different from the three human pathogenic species B. burgdorferi sensu stricto, Borrelia garinii, andBorrelia afzelii, as well as from other B. burgdorferi sensu lato species. Our findings indicate thatBorrelia genomic groups or isolates other than the three well-known human pathogenic species may also cause human Lyme borreliosis.
Acyclicity over local rings with radical cube zero

This paper studies infinite acyclic complexes of finitely generated free modules over a commutative noetherian local ring $(R,m)$ with $m^3=0$. Conclusive results are obtained on the growth of the ranks of the modules in acyclic complexes, and new sufficient conditions are given for total acyclicity. Results are also obtained on the structure of rings that admit acyclic complexes; part of this structure is exhibited by every ring $R$ that admits a non-free finitely generated non-zero module $M$ with $Ext^n(M,R)=0$ for a few $n>0$.
A comparison of related donor peripheral blood and bone marrow transplants: importance of late-onset chronic graft-versus-host disease and infections.

Peripheral blood hematopoietic stem cell (PBSC) transplants have been shown to result in more rapid engraftment than standard bone marrow transplants (BMTs). Little comparative data exist regarding complications in patients receiving transplants using these stem cell sources. In our study, 97 adults with advanced hematologic malignancies who received allogeneic PBSC transplants were compared with 97 adults who received allogeneic BMTs using identical preparative regimens and support parameters. The incidence of systemic infections and other major complications occurring within the first year after transplantation were calculated in both groups. Proportional hazard analysis was used to examine risk factors for death and complications in both groups. Patients receiving PBSC transplants had more rapid neutrophil (17 days versus 24 days; P <.001) and platelet engraftment (28 days versus 47 days; P <.001) than BMT recipients. The survival rate at 2 years was 38% in PBSC transplant recipients and 28% in marrow recipients (P =.08). There was no difference in rates of grade II to IV acute graft-versus-host disease (GVHD) between groups (PBSC 46%, BMT 51%; P =.3). PBSC transplant recipients were more likely to develop chronic GVHD after 180 days (hazard ratio 2.2; P =.05). Accompanying this "late-onset chronic GVHD," a pattern of more frequent late systemic fungal and cytomegalovirus infections was observed in PBSC transplant recipients. In conclusion, although PBSC transplant recipients engraft more quickly than BMT recipients and have somewhat better 2-year survival rates, they develop more frequent late-onset chronic GVHD and may have more late fungal and cytomegalovirus infections than marrow recipients. Further studies must examine this late-onset chronic GVHD and better characterize immune reconstitution in PBSC transplant recipients to understand their effects on patient recovery.
Identification of the Distance between the Homologous Halves of P-glycoprotein That Triggers the High/Low ATPase Activity Switch*

Background: The mechanism for activation of P-glycoprotein ATPase activity during its reaction cycle is unknown. Results: Cross-linking the homologous halves within 6–19 Å highly activated ATPase activity but cross-linking >20 Å did not. Conclusion: ATPase activation switch is turned on when halves clamped at less than 20 Å. Significance: A key conformational switch in the P-gp reaction cycle is identified. P-glycoprotein (P-gp, ABCB1) is an ATP-binding cassette drug pump that protects us from toxic compounds and confers multidrug resistance. Each homologous half contains a transmembrane domain with six transmembrane segments followed by a nucleotide-binding domain (NBD). The drug- and ATP-binding sites reside at the interface between the transmembrane domain and NBDs, respectively. Drug binding activates ATPase activity by an unknown mechanism. There is no high resolution structure of human P-gp, but homology models based on the crystal structures of bacterial, mouse, and Caenorhabditis elegans ATP-binding cassette drug pumps yield both open (NBDs apart) and closed (NBDs together) conformations. Molecular dynamics simulations predict that the NBDs can be separated over a range of distances (over 20 Å). To determine the distance that show high or low ATPase activity, we cross-linked reporter cysteines L175C (N-half) and N820C (C-half) with cross-linkers of various lengths that separated the halves between 6 and 30 Å (α-carbons). We observed that ATPase activity increased over 10-fold when the cysteines were cross-linked at distances between 6 and 19 Å, although cross-linking at distances greater than 20 Å yielded basal levels of activity. The results suggest that the ATPase activation switch appears to be turned on or off when L175C/N820 are clamped at distances less than or greater than 20 Å, respectively. We predict that the high/low ATPase activity switch may occur at a distance where the NBDs are predicted in molecular dynamic simulations to undergo pronounced twisting as they approach each other (Wise, J. G. (2012) Biochemistry 51, 5125–5141).
Human Visual System Automatically Encodes Sequential Regularities of Discrete Events

For our adaptive behavior in a dynamically changing environment, an essential task of the brain is to automatically encode sequential regularities inherent in the environment into a memory representation. Recent studies in neuroscience have suggested that sequential regularities embedded in discrete sensory events are automatically encoded into a memory representation at the level of the sensory system. This notion is largely supported by evidence from investigations using auditory mismatch negativity (auditory MMN), an event-related brain potential (ERP) correlate of an automatic memory-mismatch process in the auditory sensory system. However, it is still largely unclear whether or not this notion can be generalized to other sensory modalities. The purpose of the present study was to investigate the contribution of the visual sensory system to the automatic encoding of sequential regularities using visual mismatch negativity (visual MMN), an ERP correlate of an automatic memory-mismatch process in the visual sensory system. To this end, we conducted a sequential analysis of visual MMN in an oddball sequence consisting of infrequent deviant and frequent standard stimuli, and tested whether the underlying memory representation of visual MMN generation contains only a sensory memory trace of standard stimuli (trace-mismatch hypothesis) or whether it also contains sequential regularities extracted from the repetitive standard sequence (regularity-violation hypothesis). The results showed that visual MMN was elicited by first deviant (deviant stimuli following at least one standard stimulus), second deviant (deviant stimuli immediately following first deviant), and first standard (standard stimuli immediately following first deviant), but not by second standard (standard stimuli immediately following first standard). These results are consistent with the regularity-violation hypothesis, suggesting that the visual sensory system automatically encodes sequential regularities. In combination with a wide range of auditory MMN studies, the present study highlights the critical role of sensory systems in automatically encoding sequential regularities when modeling the world.
Autoimmune hepatitis/sclerosing cholangitis overlap syndrome in childhood: A 16‐year prospective study

To investigate whether sclerosing cholangitis with an autoimmune serology characteristic of autoimmune hepatitis (AIH) and AIH are distinct entities, we studied 55 consecutive children with clinical and/or biochemical evidence of liver disease and circulating antinuclear (ANA), anti‐smooth muscle (SMA), and/or liver‐kidney‐microsomal type 1 (LKM1) autoantibodies. They underwent liver biopsy, direct cholangiography, sigmoidoscopy, and rectal biopsy at presentation. Twenty‐eight were diagnosed as AIH in the absence and 27 autoimmune sclerosing cholangitis (ASC) in the presence of radiological features of cholangiopathy. Twenty‐six ASC and 20 AIH had ANA and/or SMA; 1 ASC and 8 AIH LKM1 autoantibody. Similarities between the 2 conditions included most clinical and biochemical parameters and a lower frequency of HLA DR4. Inflammatory bowel disease and histological biliary changes were more common in ASC; coagulopathy, hypoalbuminemia, lymphocytic periportal hepatitis, and HLA DR3 were more common in AIH. Histological biliary changes were observed in 65% of ASC and 31% of AIH patients. Eighty‐nine percent responded to immunosuppression. Follow‐up liver biopsies from 17 ASC and 18 AIH patients had similarly reduced inflammatory activity and no progression to cirrhosis. Sixteen follow‐up cholangiograms from AIH patients and 9 from ASC patients were unchanged, while 8 ASC patients showed a progressive cholangiopathy. One child with AIH and ulcerative colitis developed sclerosing cholangitis 8 years after presentation. At 2 to 16 years (median, 7 years) from presentation, all patients are alive, including 4 ASC patients who underwent liver transplantation. In conclusion, ASC and AIH are similarly prevalent in childhood; cholangiography is often needed to distinguish between these 2 entities, which are likely to lie within the same disease process.S
SoftFLASH: analyzing the performance of clustered distributed virtual shared memory

One potentially attractive way to build large-scale shared-memory machines is to use small-scale to medium-scale shared-memory machines as clusters that are interconnected with an off-the-shelf network. To create a shared-memory programming environment across the clusters, it is possible to use a virtual shared-memory software layer. Because of the low latency and high bandwidth of the interconnect available within each cluster, there are clear advantages in making the clusters as large as possible. The critical question then becomes whether the latency and bandwidth of the top-level network and the software system are sufficient to support the communication demands generated by the clusters.To explore these questions, we have built an aggressive kernel implementation of a virtual shared-memory system using SGI multiprocessors and 100Mbyte/sec HIPPI interconnects. The system obtains speedups on 32 processors (four nodes, eight processors per node plus additional reserved protocol processors) that range from 6.9 on the communication-intensive FFT program to 21.6 on Ocean (both from the SPLASH 2 suite). In general, clustering is effective in reducing internode miss rates, but as the cluster size increases, increases in the remote latency, mostly due to increased TLB synchronization cost, offset the advantages. For communication-intensive applications, such as FFT, the overhead of sending out network requests, the limited network bandwidth, and the long network latency prevent the achievement of good performance. Overall, this approach still appears promising, but our results indicate that large low latency networks may be needed to make cluster-based virtual shared-memory machines broadly useful as large-scale shared-memory multiprocessors.
Event-Driven Nonlinear Discounted Optimal Regulation Involving a Power System Application

By employing neural network approximation architecture, the nonlinear discounted optimal regulation is handled under event-driven adaptive critic framework. The main idea lies in adopting an improved learning algorithm, so that the event-driven discounted optimal control law can be derived via training a neural network. The stability guarantee and simulation illustration are also included. It is highlighted that the initial stabilizing control policy is not required during the implementation process with the combined learning rule. Moreover, the closed-loop system is formulated as an impulsive model. Then, the related stability issue is addressed by using the Lyapunov approach. The simulation studies, including an application to a power system, are also conducted to verify the effectiveness of the present design method.
Clinicopathologic characteristics of five autopsied cases of dura mater‐associated Creutzfeldt‐Jakob disease

We present five cases of dura mater‐associated Creutzfeldt‐Jakob disease (dura‐CJD) that were analyzed clinicopathologically and review previous reports. The average age at dura mater transplantation was 54.4 ± 7.3 years, and the average age at CJD onset was 66.0 ± 8.2 years, with an average latency period of 11.6 ± 1.1 years. The average age at death was 67.6 ± 8.7 years, with an average CJD disease duration of 16.8 ± 10.4 months. Symptoms of CJD onset in four patients who received dura mater transplantation below the cerebellar tent reflected cerebellar or brainstem dysfunction, whereas symptoms of one patient who received transplantation above the cerebellar tent reflected cerebral cortical involvement. All patients showed rapidly progressive cognitive impairment, and both periodic sharp‐wave complexes on electroencephalogram and myoclonus were observed in the early disease stage. Neuropathologic evaluation showed one case of subacute spongiform encephalopathy and four cases of panencephalopathic‐type CJD. Widespread cerebral neocortical, subcortical gray matter and cerebellar cortical involvement were observed to varying degrees, and severity tended to be associated with CJD disease duration. There were no instances of kuru plaques or florid plaques. Prion protein (PrP) immunostaining showed widespread synaptic‐type PrP deposition. No differences between our dura‐CJD cases and typical cases of sporadic CJD were found with respect to clinicopathologic findings, except history of dura mater transplantation. Although a specific association between the dura mater graft site and neuropathologic observations was not evaluated in the present study, the initial symptoms appear to be closely related to the graft site, indicating a direct transmission of CJD from the graft site to the adjacent brain.