Miracle-dz/UniFAR-FTUs huggingface dataset:
Butyrate as an effective treatment of congenital chloride diarrhea.

BACKGROUND & AIMS Many therapeutic attempts have demonstrated to be ineffective in reducing the severity of congenital chloride diarrhea and its long-term complications. The short-chain fatty acid butyrate stimulates intestinal water and ion absorption through a variety of mechanisms, including the activation of a parallel Cl-/butyrate and Na+/H+ exchanger. In this case report, we report the therapeutic efficacy of butyrate on an 11-year-old patient affected by congenital chloride diarrhea. METHODS The efficacy of increasing doses of oral butyrate (from 50 to 100 mg/kg/day) was investigated through the daily evaluation of stool volume, bowel movements, fecal incontinence, serum, and stool electrolytes concentrations. The modifications in transepithelial intestinal ion transport elicited by butyrate were examined by rectal dialysis study. RESULTS A butyrate dose of 100 mg/kg/day induced a normalization of stool pattern and of serum and fecal electrolytes concentration. The rectal dialysis study demonstrated a proabsorptive effect induced by butyrate on Na+, Cl-, and K+ intestinal transport. Butyrate therapy was well tolerated during the entire 12-month observation period, and the stool pattern and fecal and serum ion concentrations remained stable within the normal ranges. No clinical adverse events or episodes of dehydration requiring hospital care were observed. CONCLUSIONS Butyrate could be effective in treating congenital chloride diarrhea. It is easily administered, useful in preventing severe dehydration episodes, and may be a promising therapeutic approach for a long-term treatment in this rare and severe condition.
Potential Cooperations between Odorant-Binding Proteins of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)

It was previously thought that the odorant binding proteins (OBPs) in the sensillum lymph might serve as carriers, which could carry lipophilic odorant molecules to olfactory receptors. In this study, two novel OBP genes of the scarab beetle Holotrichia oblita were screened using an antennal cDNA library. The full cDNA of HoblOBP3 and HoblOBP4 was cloned using reverse transcription PCR and rapid amplification of the cDNA ends. Homology modeling of both OBPs was performed using SWISS-MODEL on-line tools. Next, the two OBPs were expressed in Escherichia coli and purified using Ni ion affinity chromatography. The ligand-binding properties of HoblOBP3 and HoblOBP4 in 42 ligands respectively were measured using the fluorescence probe N-phenyl-naphthylamine (1-NPN). The results obtained from competitive binding assays demonstrated that HoblOBP4 showed a broader range of binding affinities to the test compounds, while HoblOBP3 displays more specific binding affinity. Furthermore, other OBPs and CSPs were expressed in Escherichia coli and purified using Ni ion affinity chromatography. Binding curves were measured for binary mixtures of OBPs and CSPs using 1-NPN, and the Scatchard plots exhibited “J”-like nonlinear correlation trends in some samples. In addition, competitive binding assays of the HoblOBP1 and HoblOBP2 mixtures and of the HoblOBP2 and HoblOBP4 mixtures with representative compounds unexpectedly demonstrated good affinity, which revealed extreme differences that were only obtained using the individual proteins. In the immunocytochemical analysis, colocalization of HoblOBP1 and HoblOBP2, and of HoblOBP2 and HoblOBP4, was detected in the sensilla basiconica and sensilla placodea, respectively. All of these results suggested that HoblOBP1 and HoblOBP2, as well as HoblOBP2 and HoblOBP4, may serve as heterodimers in the sensillum lymph.
Desistance and dynamic risk factors belong together

ABSTRACT Depending on definitions, dynamic risk factors and desistance are either highly intertwined or aligned with distinctly separate paradigms. This paper describes and critiques each concept, and then reviews research on how they may be linked, including some preliminary findings from a longitudinal study of the early phases of desistance in high-risk offenders. I argue that seeking to understand how reductions in dynamic risk work together with the development of the psychological components of desistance will shed the most light on how offenders move from persistence to the maintenance of desistance.
Apg14p and Apg6/Vps30p Form a Protein Complex Essential for Autophagy in the Yeast, Saccharomyces cerevisiae *

Mutation in the Saccharomyces cerevisiae APG14 gene causes a defect in autophagy. Cloning and structural analysis of the APG14 gene revealed that APG14 encodes a novel hydrophilic protein with a predicted molecular mass of 40.5 kDa, and that Apg14p has a coiled-coil motif at its N terminus region. We found that overproduction of Apg14p partially reversed the defect in autophagy induced by theapg6-1 mutation. The apg6-1 mutant was found to be defective not only in autophagy but also in sorting of carboxypeptidase Y (CPY), a vacuolar-soluble hydrolase, to the vacuole. However, overexpression of APG14 did not alter the CPY sorting defect of the apg6-1 mutant, nor did theapg14 null mutation affect the CPY sorting pathway. Structural analysis of APG6 revealed that APG6is identical to VPS30, which is involved in a retrieval step of the CPY receptor, Vps10p, to the late-Golgi from the endosome (Seaman, M. N. J., Marcusson, E. G., Cereghino, J. L., and Emr, S. D. (1997) J. Cell Biol. 137, 79–92). Subcellular fractionation indicated that Apg14p and Apg6p peripherally associated with a membrane structure(s). Apg14p was co-immunoprecipitated with Apg6p, suggesting that they form a stable protein complex. These results imply that Apg6/Vps30p has two distinct functions in the autophagic process and the vacuolar protein sorting pathway. Apg14p may be a component specifically required for the function of Apg6/Vps30p through the autophagic pathway.
Critical Review: Is it beneficial for children awaiting a cochlear implant to be taught sign language prior to implantation?

This critical review examines the efficacy of teaching sign language to children awaiting cochlear implantation. A holistic approach to development is utilized so children’s later mode of communication, speech characteristics and psychosocial development are considered. Study designs include: prospective or retrospective cohort studies (3), within group repeated measures (1), and expert opinion (1). Based on the evidence provided, pre-implant sign language use does not provide a measurable advantage or determent to cochlear implant users. Parents should be advised to choose the pre-implant mode of communication they are most comfortable and proficient with, as this will allow them to provide their child with a rich language environment prior to cochlear implantation.
Factors Contributing to Teachers' Judgments about Students and Decisions about Grouping Students for Reading Instruction

In a policy-capturing study of the planning of reading instruction, teachers and student teachers read descriptions of 32 hypothetical students who varied on five dimensions: standardized reading achievement score, self-correction of errors during oral reading, class participation, social competence, and classroom behavior. They judged each student's probable mastery of the reading curriculum and impact on classroom life, placed the students in three reading groups, and described the reading groups and strategies used to form these groups. While most participants used the academic cues in judging mastery and forming reading groups and the nonacademic cues in estimating impact on classroom life, decision policies differed with respect to the number of cues used and size of each cue's effect. Some variability in cue use was explained by teaching experience and conceptions of reading. For example, teachers' policies were more complex than those of student teachers for the mastery and grouping decisions. However, no differences were apparent for the impact judgment. Additional insight into differences in grouping strategies was provided by participants' written descriptions of reading groups and strategies used to form these groups.
Correction: Association of birth weight with corneal power in early adolescence: Results from the National Health and Nutrition Examination Survey (NHANES) 1999-2008

Purpose To analyze the effect of birth weight on ocular morphology, refraction and visual function in early adolescents aged 12–15 years. Material and methods We conducted a secondary data analysis using the public use files from the National Health and Nutrition Examination Survey of the period from 1999 to 2008. Study participants aged 12 to 15 years were included with data on birth weight and ophthalmic parameters including presenting distance visual acuity, objective refraction and keratometry. Visual acuity, sphere, astigmatism in power vectors J0 and J45, corneal power and corneal astigmatism were evaluated for an association with birth weight. Linear and logistic regression with adjustment for age, sex, ethnicity, survey cycle and birth weight as independent variable were calculated. Results Linear regression analysis revealed an association between corneal power and birth weight (per 100g: beta = -0.04, p<0.001) in the univariate analysis, and in the model adjusted for age, sex, ethnicity and NHANES survey cycle (per 100g: beta = -0.04, p<0.001). A lower birth weight was associated with higher corneal power. We found no evidence for an association of visual acuity, sphere, spherical equivalent, J0-vector and J45-vector of astigmatism, corneal J0- or corneal J45-vector with birth weight. Conclusion Our data demonstrate that low BW is linked to alterations in keratometric power even in early adolescents aged 12–15 years whereas visual acuity and refractive error showed no association.
RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism

Xyr1 has been demonstrated to be the main transcription activator of (hemi)cellulases in the well-known cellulase producer Trichoderma reesei. This study comprehensively investigates the genes regulated by Xyr1 through RNA sequencing to produce the transcription profiles of T. reesei Rut-C30 and its xyr1 deletion mutant (Δxyr1), cultured on lignocellulose or glucose. xyr1 deletion resulted in 467 differentially expressed genes on inducing medium. Almost all functional genes involved in (hemi)cellulose degradation and many transporters belonging to the sugar porter family in the major facilitator superfamily (MFS) were downregulated in Δxyr1. By contrast, all differentially expressed protease, lipase, chitinase, some ATP-binding cassette transporters, and heat shock protein-encoding genes were upregulated in Δxyr1. When cultured on glucose, a total of 281 genes were expressed differentially in Δxyr1, most of which were involved in energy, solute transport, lipid, amino acid, and monosaccharide as well as secondary metabolism. Electrophoretic mobility shift assays confirmed that the intracellular β-glucosidase bgl2, the putative nonenzymatic cellulose-attacking gene cip1, the MFS lactose transporter lp, the nmrA-like gene, endo T, the acid protease pepA, and the small heat shock protein hsp23 were probable Xyr1-targets. These results might help elucidate the regulation system for synthesis and secretion of (hemi)cellulases in T. reesei Rut-C30.
Comparison of gait in patients following a computer‐navigated minimally invasive anterior approach and a conventional posterolateral approach for total hip arthroplasty: A randomized controlled trial

Minimally invasive total hip arthroplasty (MIS THA) aims at minimizing damage to muscles and tendons to accelerate postoperative recovery. Computer navigation allows a precise prosthesis alignment without complete visualization of the bony landmarks during MIS THA. A randomized controlled trial (RCT) was conducted to determine the effectiveness of a computer‐navigated MIS anterior approach for THA compared to a conventional posterolateral THA technique on the restoration of physical functioning during recovery following surgery. Thirty‐five patients underwent computer‐navigated MIS THA via the anterior approach, and 40 patients underwent conventional THA using the conventional posterolateral approach. Gait analysis was performed preoperatively, 6 weeks, and 3 and 6 months postoperatively using a body‐fixed‐sensor based gait analysis system. Walking speed, step length, cadence, and frontal plane angular movements of the pelvis and thorax were assessed. The same data were obtained from 30 healthy subjects. No differences were found in the recovery of spatiotemporal parameters or in angular movements of the pelvis and thorax following the computer‐navigated MIS anterior approach or the conventional posterolateral approach. Although gait improved after surgery, small differences in several spatiotemporal parameters and angular movements of the trunk remained at 6 months postoperatively between both patient groups and healthy subjects. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 288–294, 2013
Enzymes Involved in Anaerobic Polyethylene Glycol Degradation by Pelobacter venetianus and Bacteroides Strain PG1

In extracts of polyethylene glycol (PEG)-grown cells of the strictly anaerobically fermenting bacterium Pelobacter venetianus, two different enzyme activities were detected, a diol dehydratase and a PEG-degrading enzyme which was characterized as a PEG acetaldehyde lyase. Both enzymes were oxygen sensitive and depended on a reductant, such as titanium citrate or sulfhydryl compounds, for optimal activity. The diol dehydratase was inhibited by various corrinoids (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, and methylcobalamin) by up to 37% at a concentration of 100 μM. Changes in ionic strength and the K+ ion concentration had only limited effects on this enzyme activity; glycerol inhibited the enzyme by 95%. The PEG-degrading enzyme activity was stimulated by the same corrinoids by up to 80%, exhibited optimal activity in 0.75 M potassium phosphate buffer or in the presence of 4 M KCI, and was only slightly affected by glycerol. Both enzymes were located in the cytoplasmic space. Also, another PEG-degrading bacterium, Bacteroides strain PG1, contained a PEG acetaldehyde lyase activity analogous to the corresponding enzyme of P. venetianus but no diol dehydratase. Our results confirm that corrinoid-influenced PEG degradation analogous to a diol dehydratase reaction is a common strategy among several different strictly anaerobic PEG-degrading bacteria.
Establishing the validity and reliability of course evaluation questionnaires

This article uses the case of designing a new course questionnaire to discuss the issues of validity, reliability and diagnostic power in good questionnaire design. Validity is often not well addressed in course questionnaire design as there are no straightforward tests that can be applied to an individual instrument. The authors propose the technique of establishing validity by deriving constructs from naturalistic qualitative research—in this case by interviewing award‐winning teachers about their principles and practices. Analysis of the interview transcripts led to nine principles of good teaching, which were developed into nine questionnaire scales. Reliability was tested with Cronbach’s alpha and with confirmatory factor analysis, as the use of Cronbach’s alpha alone can mask issues of multi‐dimensionality in scales. The concept of diagnostic power as the ability of an instrument to distinguish between related constructs is introduced. This is important in course evaluation questionnaires, as it enables relative strengths and weaknesses to be identified, which makes it possible to advise on remedial action.
The Hepatitis C Virus NS2 Protein Is an Inhibitor of CIDE-B-induced Apoptosis*

Chronic hepatitis C virus (HCV) infection frequently leads to liver cancer. To determine the viral factor(s) potentially involved in viral persistence, we focused our work on NS2, a viral protein of unknown function. To assign a role for NS2, we searched for cellular proteins that interact with NS2. Performing a two-hybrid screen on a human liver cDNA library, we found that NS2 interacted with the liver-specific pro-apoptotic CIDE-B protein. Binding specificity of NS2 for CIDE-B was confirmed by cell-free assays associated with colocalization studies and coprecipitation experiments on human endogenous CIDE-B. CIDE-B, a member of the novel CIDE family of apoptosis-inducing factors, has been reported to show strong cell death-inducing activity in its C-terminal domain. We show that this CIDE-B killing domain is involved in the NS2 interaction. NS2 binding was sufficient to inhibit CIDE-B-induced apoptosis because an NS2 deletion mutant unable to interact with CIDE-B in vitrolost its capacity to interfere with CIDE-B cell death activity. Although it has been reported that CIDE-B-induced apoptosis is characterized by mitochondrial localization, the precise apoptotic mechanism remained unknown. Here, we show that CIDE-B induced cell death in a caspase-dependent manner through cytochromec release from mitochondria. Furthermore, we found that NS2 counteracted the cytochrome c release induced by CIDE-B.In vivo, the CIDE-B protein level was extremely low in adenovirus-infected transgenic mice expressing the HCV polyprotein compared with that in wild-type mice. We suggest that NS2 interferes with the CIDE-B-induced death pathway and participates in HCV strategies to subvert host cell defense.
Peer Assessment in Undergraduate Programmes

Abstract Peer assessment was introduced into the assessment strategy of two marketing modules on two undergraduate programmes at Manchester Metropolitan University. A questionnaire was used to elicit responses from a sample of 80 students concerning their attitudes to and experience of, the peer assessment exercise. Students felt that the benefits of peer assessment were: the increase in personal motivation as a result of their active involvement in the assessment process; the opportunity to compare and discuss the assignment, and the opportunity to gain knowledge and develop a greater understanding of the assignment content and assessment process. Criticisms included the effects of personal bias on the marks awarded, the interpretation of criteria and the ability of the students to assess. Some students regarded peer assessment as an incentive to perform, while others saw it as an unfair system that lacked objectivity. Over half the sample regarded assessment as solely a role for the tutor. The research .
Dynamics and monitoring of mind-wandering

Humans’ conscious experience is always changing: now seeing, now remembering, now planning. Yet, how people notice the unfolding of their own thoughts remains unclear. The present thesis studied the dynamics and introspective monitoring of thoughts, by capitalizing on the salient and often unnoticed phenomenon of mind-wandering (Chapter 1). Mind-wandering is the rise of thoughts about personal concerns. Against the idea that this rise is unpredictable, I first describe the characteristics of slow rhythms that predict mind-wandering occurrences during goal-directed tasks (Ch. 2, 3). Interestingly, these dynamics may determine thought content and introspection. Indeed, patients with Attention deficits (ADHD), who would likely experience alterations in both the dynamics and introspection of mind-wandering, report more “empty thoughts” (Ch. 4). Also, two experiments and a study using smartphones suggest that inner speech facilitates awareness of mind-wandering (Ch. 5). Inner speech contributes to monitoring capacities, thus verbal thoughts could be better sustained in time, increasing their vividness. Finally, I suggest that the detection of inner thoughts is not a direct access to clear-cut elements, but rather an adjustable decision mechanism applied to internal continuous and dynamic variables, similar to what happens in perception (Ch. 6). In sum, these studies shed a new light on human’s introspection and confirm old insights (Ch. 7). Indeed, they provide empirical support to a philosophical claim made 125 years ago by William James, one of the fathers of scientific psychology: we experience a continuous stream of thought, and detecting mind-wandering may depend on these dynamics.
Assessment and treatment of aggressive children, from a social-cognitive perspective

The development and psychometric evaluation of the Social Cognitive Skills Test (SCST) for (aggressive) children is described. The SCST is based on the structural developmental approach of social cognition by Selman and Byrne (1974) and consists of six short stories with corresponding pictures. Each story measures eight social cognitive skills. The SCST takes into account the developmental level of the (aggressive) child and his/her social information processing deficits. Three studies were conducted to explore the psychometric qualities of the SCST. Study 1 (n = 47) supported the notion that (a) the SCST discriminates between aggressive and non-aggressive children, and that (b) there is a descending trend in SCST-scores as social cognitive skills increase in complexity. Study 2 (n = 115) confirmed the findings of Study 1 and further revealed (a) no differences in the scores on the SCST between reactive and proactive aggressive children, and (b) a positive association between chronological age and social cognitive level of the aggressive and non-aggressive children. Finally, Study 3 (n = 48) confirmed the previous findings and found that 1 Published in: Clinical Psychology and Psychotherapy, 8, 341-351, October, 2001 Social Cognitive Skills Test 24 aggressive children have difficulty verbalizing their thoughts, feelings and intentions and show a lack in non-verbal social understanding.
CHINESE AND CHINESE-AMERICAN FAMILIES OF CHILDREN WITH DISABILITIES

Cultural characteristics impact on parents’ attitudes and perceptions toward children with disabilities. Therefore the unique family background could create a challenge for service providers. Professional who works with children with disabilities need information from families in order to provide effective intervention(Parette, Summer 2004). The purpose of this paper is to review the perception and challenges ChineseAmerican families of children with disabilities encounter. Specific emphasis was placed on the influence of acculturation of Chinese-American and the intervention of professionals in educational decision making process.