Miracle-dz/UniFAR-FTUs huggingface dataset:
A Summating, Exponentially-Decaying CMOS Synapse for Spiking Neural Systems

Synapses are a critical element of biologically-realistic, spike-based neural computation, serving the role of communication, computation, and modification. Many different circuit implementations of synapse function exist with different computational goals in mind. In this paper we describe a new CMOS synapse design that separately controls quiescent leak current, synaptic gain, and time-constant of decay. This circuit implements part of a commonly-used kinetic model of synaptic conductance. We show a theoretical analysis and experimental data for prototypes fabricated in a commercially-available 1. 5µm CMOS process.
HIV-1 infection alters CD4+ memory T-cell phenotype at the site of disease in extrapulmonary tuberculosis

HIV‐1‐infected people have an increased risk of developing extrapulmonary tuberculosis (TB), the immunopathogenesis of which is poorly understood. Here, we conducted a detailed immunological analysis of human pericardial TB, to determine the effect of HIV‐1 co‐infection on the phenotype of Mycobacterium tuberculosis (MTB)‐specific memory T cells and the role of polyfunctional T cells at the disease site, using cells from pericardial fluid and blood of 74 patients with (n=50) and without (n=24) HIV‐1 co‐infection. The MTB antigen‐induced IFN‐γ response was elevated at the disease site, irrespective of HIV‐1 status or antigenic stimulant. However, the IFN‐γ ELISpot showed no clear evidence of increased numbers of antigen‐specific cells at the disease site except for ESAT‐6 in HIV‐1 uninfected individuals (p=0.009). Flow cytometric analysis showed that CD4+ memory T cells in the pericardial fluid of HIV‐1‐infected patients were of a less differentiated phenotype, with the presence of polyfunctional CD4+ T cells expressing TNF, IL‐2 and IFN‐γ. These results indicate that HIV‐1 infection results in altered phenotype and function of MTB‐specific CD4+ T cells at the disease site, which may contribute to the increased risk of developing TB at all stages of HIV‐1 infection.
Evaluation of the osteogenic potential of growth factor-rich demineralized bone matrix in vivo.

BACKGROUND The study evaluates the osteogenic properties and biocompatibility of growth factor-rich demineralized bone matrix (GDBM) by comparing with cancellous mineralized bone matrix (CMBM) and anorganic bovine bone matrix (ABBM). METHODS Thirty-six Sprague-Dawley rats were used (n = 6/group/time point). To assess biocompatibility and osteoinductivity, the respective bone matrices were randomly placed in subcutaneous pouches for 7 and 28 days and evaluated by histology and osteopontin expression. Osteoconductivity was assessed by randomly implanting respective bone matrices in osteotomies on femurs for 14 and 28 days and evaluated by microcomputed tomography and histology. RESULTS Neither acute inflammation nor mineralized tissue was noted in any of the subcutaneous specimens, whereas expression of osteopontin was more prominent in the GDBM group. Among the femoral specimens, the greatest relative bone volume (bone volume [BV] divided by trabecular volume [TV]) and trabecular thickness was noted in the ABBM group at both time points, whereas less BV/TV was noted in GDBM group at day 14. Residual matrix particles were noted in all examined groups at both time points, without significant differences regarding defect fill between groups. The GDBM group presented similar levels of newly formed bone compartment and marrow space to those of the ABBM group. CONCLUSIONS GDBM demonstrated acceptable biocompatibility and osteogenic potential comparable to ABBM in vivo. Further investigations in a more clinically relevant model are warranted.
Quality in Early Childhood Programs: Reflections from Program Evaluation Practices

This study investigates how program evaluation has contributed to the definition of quality in early childhood programs and what social and cultural conditions have influenced these definitions. First, a review of evaluation reports from the last 3 decades identified three types of quality used to evaluate early childhood programs: (a) outcome-determined quality, (b) standards-based quality, and (c) developmental appropriateness. An analysis of questionnaire responses from evaluators and interviews with early childhood practitioners was also conducted in order to explore underlying social and cultural conditions of each quality definition. The authors conclude that, in order to be valid and meaningful, evaluation of early childhood programs should be based on in-depth understanding of dynamic program processes and diverse stakeholders’ perspectives on program quality. Evaluation should challenge and expand the dominant perspectives on early childhood program quality.
Differentiation of Arabidopsis Guard Cells: Analysis of the Networks Incorporating the Basic Helix-Loop-Helix Transcription Factor, FAMA1[C][W][OA]

Nearly all extant land plants possess stomata, the epidermal structures that mediate gas exchange between the plant and the environment. The developmental pathways, cell division patterns, and molecules employed in the generation of these structures are simple examples of processes used in many developmental contexts. One specific module is a set of “master regulator” basic helix-loop-helix transcription factors that regulate individual consecutive steps in stomatal development. Here, we profile transcriptional changes in response to inducible expression of Arabidopsis (Arabidopsis thaliana) FAMA, a basic helix-loop-helix protein whose actions during the final stage in stomatal development regulate both cell division and cell fate. Genes identified by microarray and candidate approaches were then further analyzed to test specific hypothesis about the activity of FAMA, the shape of its regulatory network, and to create a new set of stomata-specific or stomata-enriched reporters.
Barnase as a New Therapeutic Agent Triggering Apoptosis in Human Cancer Cells

Background RNases are currently studied as non-mutagenic alternatives to the harmful DNA-damaging anticancer drugs commonly used in clinical practice. Many mammalian RNases are not potent toxins due to the strong inhibition by ribonuclease inhibitor (RI) presented in the cytoplasm of mammalian cells. Methodology/Principal Findings In search of new effective anticancer RNases we studied the effects of barnase, a ribonuclease from Bacillus amyloliquefaciens, on human cancer cells. We found that barnase is resistant to RI. In MTT cell viability assay, barnase was cytotoxic to human carcinoma cell lines with half-inhibitory concentrations (IC50) ranging from 0.2 to 13 µM and to leukemia cell lines with IC50 values ranging from 2.4 to 82 µM. Also, we characterized the cytotoxic effects of barnase-based immunoRNase scFv 4D5-dibarnase, which consists of two barnase molecules serially fused to the single-chain variable fragment (scFv) of humanized antibody 4D5 that recognizes the extracellular domain of cancer marker HER2. The scFv 4D5-dibarnase specifically bound to HER2-positive cells and was internalized via receptor-mediated endocytosis. The intracellular localization of internalized scFv 4D5-dibarnase was determined by electronic microscopy. The cytotoxic effect of scFv 4D5-dibarnase on HER2-positive human ovarian carcinoma SKOV-3 cells (IC50 = 1.8 nM) was three orders of magnitude greater than that of barnase alone. Both barnase and scFv 4D5-dibarnase induced apoptosis in SKOV-3 cells accompanied by internucleosomal chromatin fragmentation, membrane blebbing, the appearance of phosphatidylserine on the outer leaflet of the plasma membrane, and the activation of caspase-3. Conclusions/Significance These results demonstrate that barnase is a potent toxic agent for targeting to cancer cells.
Safetalk Revisited, or: Language and Ideology in Luxembourgish Educational Policy

In this paper, I first review previous research on the topic of safetalk. I then apply some of the insights derived from this discussion to a case study of the Luxembourgish primary school system. The study uses both ethnographic and discourse analysis, and focuses in particular on the role of French language varieties in a system that forces large numbers of romanophone students to go through a German-language literacy programme. The picture that emerges reveals what changes would be needed to break through restrictive safetalk practices and enable more effective and more equitable learning. However, it is argued that the difficulties of implementing such changes are compounded by a number of language ideologies that underpin both preschool and primary school education in Luxembourg.
Comparison of the effects of a balanced crystalloid‐based and a saline‐based tetrastarch solution on canine whole blood coagulation and platelet function

OBJECTIVE To evaluate the effects of a 6% hydroxyethyl starch (130/0.42) solution in either a buffered, electrolyte-balanced (HES-BAL), or a saline (HES-SAL) carrier solution on canine platelet function and whole blood coagulation. DESIGN Prospective, randomized study. SETTING University teaching hospital. ANIMALS Thirty-seven client-owned dogs undergoing general anesthesia for arthroscopy or imaging studies. INTERVENTIONS Dogs received a 15 mL/kg intravenous bolus of HES-SAL (n = 13), HES-BAL (n = 14), or a modified Ringer's solution (n = 10) over 30-40 minutes. Coagulation was analyzed using a Platelet Function Analyzer-100 (closure time [CtPFA ]), and whole blood thromboelastometry (ROTEM) with extrinsically (ex-tem and fib-tem) and intrinsically (in-tem) activated assays, which assessed clotting time (CT), clot formation time (CFT), maximal clot firmness (MCF), and lysis index (LI). Coagulation samples were assayed prior to fluid administration (T0), and 5 minutes (T1), and 3 hours (T2) following fluid bolus administration, respectively. RESULTS Both HES solutions resulted in impaired platelet function as indicated by a significant prolongation of CtPFA at T1 as compared to T0, but which resolved by T2. An IV bolus of Ringer's solution did not alter platelet function. In both HES groups, whole blood coagulation was significantly impaired at T1 as indicated by a significant increase in in-tem CFT, and a significant decrease in ex-tem, in-tem, and fib-tem MCF compared to T0. Furthermore, a significant increase in ex-tem CFT at T1 compared to T0 was found in the HES-SAL group. With the exception of in-tem MCF after HES-BAL, these effects were not present at T2. No significant differences were found in CtPFA or any ROTEM variable at any time point between HES-SAL and HES-BAL. CONCLUSION Administration of a single bolus of 15 mL/kg 6% HES 130/0.42 results in significant but short-lived impairment of canine platelet function and whole blood coagulation, regardless of carrier solution.
Social Determinants of Environmental Concern

Differential support for environmental issues has long provoked the curiosity of researchers. Sociodemographics and political ideology have been the primary focus of much research on the determinants of environmental concern. In light of recent work on environmental paradigms and advanced statistical methodologies, it may be advantageous to reexamine the tenets of this body of work. This study restates the findings of previous studies within a broader causal model, and tests that model using data from a general population survey. Analysis indicated that sociodemographic variables were ineffective in explaining any of three types of environmental concern measured here, but pro-regulatory liberal ideology was a strong predictor of support for environmental regulation. Further research might benefit most by exploring underlying belief structures ratherthan demographic characteristics of the population. These results emphasize the importance of careful specification of measures in studies of environmental concern.
Buccal pumping mechanics of Xenopus laevis tadpoles: effects of biotic and abiotic factors

SUMMARY Biotic factors such as body size and shape have long been known to influence kinematics in vertebrates. Movement in aquatic organisms can also be strongly affected by abiotic factors such as the viscosity of the medium. We examined the effects of both biotic factors and abiotic factors on buccal pumping kinematics in Xenopus tadpoles using high-speed imaging of an ontogenetic series of tadpoles combined with experimental manipulation of the medium over a 10-fold range of viscosity. We found influences of both biotic and abiotic factors on tadpole movements; absolute velocities and excursions of the jaws and hyoid were greater in higher viscosity fluid but durations of movements were unaffected. Smaller tadpoles have relatively wider heads and more robust hyoid muscles used in buccal expansion and compression. Lever arm ratios were found to be constant at all sizes; therefore, smaller tadpoles have relatively higher resolved muscle forces and, like tadpoles in more viscous medium, displayed higher absolute velocities of jaw and hyoid movements. Nonetheless, small tadpoles drew in water at lower Reynolds numbers (Re) than predicted by kinematics, due to negative allometry of the buccal pump. Finally, tadpoles transitioned from a flow regime dominated by viscous forces (Re=2) to an intermediate regime (Re=106).
Primary or depressive dementia: Clinical features

In 1979, Wells tabled a list of ‘major clinical features’ which he found most useful in distinguishing dementia due to organic disease from dementia occuring in the context of psychiatric conditions like depression. This article reviews recent findings on the most commonly accepted items of this list and, for several of them, concludes that the evidence is controversial. Newer aspects are then briefly considered which, when validated in longitudinal studies, may add to the clinician's range of implements during diagnostic workup.
Temperature-sensitive BC mutants of simian virus 40: block in virion assembly and accumulation of capsid-chromatin complexes

We examined the morphology, protein composition, and stability of the nucleoprotein complexes assembled in cells infected with simian virus 40 mutants belonging to the BC complementation group (tsBC11, tsBC208, tsBC214, tsB216, tsBC217, tsBC248, tsBC223, and tsBC274). We found that the 220S virions were not assembled in tsBC-infected cells under restrictive conditions. This block in assembly resulted in the accumulation of 75S chromatin in tsBC11-infected cells, as previously observed by Garber et al. (E.A. Garber, M.M. Seidman, and A.J. Levine, Virology 107:389-401, 1980). In cells infected with any other mutant listed above, the block in assembly resulted in the accumulation of 75S chromatin as well as nucleoprotein complexes sedimenting from 90 to 140S. Biochemical analysis revealed that these latter complexes contained the capsid proteins in addition to simian virus 40 DNA and the cellular core histones. Electron microscopic analysis clearly showed the association of the capsid proteins with the viral chromatin. Our results suggest that these proteins interact with simian virus 40 chromatin in the course of virion maturation and may thus play an active role in controlling simian virus 40 functions.
Translanguaging and the Writing of Bilingual Learners

This article makes the case for using translanguaging in developing the academic writing of bilinguals. It reviews the emerging literature on learning and teaching theories of translanguaging and presents theoretical understandings of biliteracy development and specifically on the teaching of writing to bilingual learners. The article analyzes five written texts produced by young bilingual writers in which translanguaging is used in the planning, drafting, and production stages of writing. It analyzes how and why translanguaging is used, as well as the effect it has in the development of writing and of voice. Translanguaging in writing is here proposed more as a self-regulating mechanism in which bilingual students can engage, rather than a pedagogy to be used in the teaching of writing itself. That is, the objective of this writing is not translanguaging itself, but the writing in one or the other constructed academic language that is the object of learning in school. Yet, the article makes it clear that even to develop the monolingual voice in writing that schools—and even bilingual schools—expect, a translanguaging approach has the most potential.
THE INTERACTION OF READING SKILLS AND SCIENCE CONTENT KNOWLEDGE WHEN TEACHING STRUGGLING SECONDARY STUDENTS

Science content area texts are difficult for most middle school students, particularly whose reading skills are below grade level. This article describes an innovative approach to integrating the teaching of middle school science content and reading skills to increase levels of student success with content area textbooks. For reading instruction, this approach features carefully selected vocabulary, word reading instruction, oral (including partner reading) and silent reading with reading fluency practice as needed, and explicit instruction on comprehension strategies, such as retelling, concept mapping, and summarization. The teaching of the science content incorporates instructional design principles that have been documented to improve comprehension of science content, process skills, and higher order thinking. Six aspects of instructional design that are discussed are the identification and teaching of big ideas, the systematic instruction of vocabulary (including science affixes), a review and integration of core concepts, visual displays of how core concepts are integrated, mnemonics for core concepts, and structured hands-on activities.
'That's what scientists have to do': Preservice elementary teachers' conceptions of the nature of science during a moon investigation

In a science methods course for elementary education majors, students investigated the phases of the moon for six weeks. The moon investigation emphasized that scientific knowledge: a) is empirically based; b) involves the invention of explanations; and c) is socially embedded. After the moon investigation, students realized that scientists make observations and generate patterns, but failed to recognize that observation could precede or follow theory building. Students could separate the processes of observing from creating explanations in their learning, but did not articulate the role of invention in science. Similarly, students valued the social dimensions of learning, but were unable to apply them to the activity of scientists. Although our teaching was explicit about students' science learning, we did not help them make direct connections between their science learning activities and the nature of science [NOS]. We provide a set of recommendations for making the NOS more explicit in the moon investigation.
The Absence of Cooperation Is Not Necessarily Defection: Structural and Motivational Constraints of Knowledge Transfer in a Social Dilemma Situation

There is a wealth of literature about knowledge transfer in general, but only a small amount deals with cultural and motivational factors of knowledge transfer. The main question of this article is: How can knowledge transfer be supported? First, we present a theoretical underpinning of knowledge transfer as a social dilemma situation. Second, we analyze how a situation of social dilemma can be overcome by means of structural and motivational solutions. Third, we provide empirical evidence from our survey in hospitals. These findings support three of our five hypotheses: direct channels for interaction, organizational culture, and intrinsic motivation are relevant requirements for knowledge transfer. Team size is not a significant factor for transferring knowledge in hospitals. Furthermore, our empirical findings differentiate between providing and obtaining knowledge. The last finding leads back to a modification of the theoretical framework.
Cognitive Function after Major Noncardiac Surgery, Apolipoprotein E4 Genotype, and Biomarkers of Brain Injury

Background:Postoperative cognitive dysfunction (POCD) is a significant cause of morbidity after noncardiac surgery. Identified risk factors are largely limited to demographic characteristics. We hypothesized that POCD was associated with apolipoprotein E4 (APOE4) genotype and plasma biomarkers of brain injury and inflammation. Methods:Three hundred ninety-four patients older than 55 yr undergoing major elective noncardiac surgery were enrolled in this prospective observational study. Apolipoprotein E genotyping was performed at baseline. Plasma was collected at baseline and end of surgery and at 4.5, 24, and 48-h postoperatively. Six protein biomarkers were assayed (B-type natriuretic peptide, C-reactive protein, D-dimer, matrix metalloproteinase-9, neuron-specific enolase, and S-100B). Neurocognitive testing was conducted at baseline and at 6 weeks and 1 yr after surgery; scores were subjected to factor analysis. The association of APOE4 and biomarkers with POCD was tested using multivariable regression modeling. Results:Three hundred fifty patients (89%) completed 6-week neurocognitive testing. POCD occurred in 54.3% of participants at 6 weeks and 46.1% at 1 yr. There was no difference in POCD between patients with or without the APOE4 allele (56.6 vs. 52.6%; P = 0.58). The continuous cognitive change score (mean ± SD) was similar between groups (APOE4: 0.05 ± 0.27 vs. non-APOE4: 0.07 ± 0.28; P = 0.53). Two hundred ninety-one subjects (74%) completed testing at 1 yr. POCD occurred in 45.9% of APOE4 subjects versus 46.3% of non-APOE4 subjects (P = 0.95). The cognitive score was again similar (APOE4: 0.08 ± 0.27 vs. non-APOE4: 0.05 ± 0.25; P = 0.39). Biomarker levels were not associated with APOE4 genotype or cognition at 6 weeks or 1 yr. Conclusion:Cognitive decline after major noncardiac surgery is not associated with APOE4 genotype or plasma biomarker levels.
Psychometric Properties of the Theory of Mind Assessment Scale in a Sample of Adolescents and Adults

This research aimed at the evaluation of the psychometric properties of the Theory of Mind Assessment Scale (Th.o.m.a.s.). Th.o. m.a.s. is a semi-structured interview meant to evaluate a person's Theory of Mind (ToM). It is composed of several questions organized in four scales, each focusing on one of the areas of knowledge in which such faculty may manifest itself: Scale A (I-Me) investigates first-order first-person ToM; Scale B (Other-Self) investigates third-person ToM from an allocentric perspective; Scale C (I-Other) again investigates third-person ToM, but from an egocentric perspective; and Scale D (Other-Me) investigates second-order ToM. The psychometric proprieties of Th.o. m.a.s. were evaluated in a sample of 156 healthy persons: 80 preadolescent and adolescent (aged 11–17 years, 42 females) and 76 adults (aged from 20 to 67 years, 35 females). Th.o. m.a.s. scores show good inter-rater agreement and internal consistency; the scores increase with age. Evidence of criterion validity was found as Scale B scores were correlated with those of an independent instrument for the evaluation of ToM, the Strange Stories task. Confirmatory factor analysis (CFA) showed good fit of the four-factors theoretical model to the data, although the four factors were highly correlated. For each of the four scales, Rasch analyses showed that, with few exceptions, items fitted the Partial credit model and their functioning was invariant for gender and age. The results of this study, along with those of previous researches with clinical samples, show that Th.o. m.a.s. is a promising instrument to assess ToM in different populations.