Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of Oseltamivir Resistance among Pandemic and Seasonal Influenza A (H1N1) Viruses by an His275Tyr Genotyping Assay Using the Cycling Probe Method

ABSTRACT Neuraminidase inhibitors are agents used against influenza viruses; however, the emergence of drug-resistant strains is a major concern. Recently, the prevalence of oseltamivir-resistant seasonal influenza A (H1N1) virus increased globally and the emergence of oseltamivir-resistant pandemic influenza A (H1N1) 2009 viruses was reported. In this study, we developed a cycling probe real-time PCR method for the detection of oseltamivir-resistant seasonal influenza A (H1N1) and pandemic influenza A (H1N1) 2009 viruses. We designed two sets of primers and probes that were labeled with 6-carboxyfluorescein or 6-carboxy-X-rhodamine to identify single nucleotide polymorphisms (SNPs) that correspond to a histidine and a tyrosine at position 275 in the neuraminidase protein, respectively. These SNPs confer susceptibility and resistance to oseltamivir, respectively. In the 2007-2008 season, the prevalence of oseltamivir-resistant H1N1 viruses was 0% (0/72), but in the 2008-2009 season, it increased to 100% (282/282). In the 2009-2010 season, all of the pandemic influenza A (H1N1) 2009 viruses were susceptible to oseltamivir (0/73, 0%). This method is sensitive and specific for the screening of oseltamivir-resistant influenza A (H1N1) viruses. This method is applicable to routine laboratory-based monitoring of drug resistance and patient management during antiviral therapy.
Nodding syndrome in Ugandan children—clinical features, brain imaging and complications: a case series

Objectives Nodding syndrome is a devastating neurological disorder of uncertain aetiology affecting children in Africa. There is no diagnostic test, and risk factors and symptoms that would allow early diagnosis are poorly documented. This study aimed to describe the clinical, electrophysiological and brain imaging (MRI) features and complications of nodding syndrome in Ugandan children. Design Case series. Participants 22 children with nodding syndrome brought to Mulago National Referral Hospital for assessment. Outcome measures Clinical features, physical and functional disabilities, EEG and brain MRI findings and a staging system with a progressive development of symptoms and complications. Results The median age of symptom onset was 6 (range 4–10) years and median duration of symptoms was 8.5 (range 2–11) years. 16 of 22 families reported multiple affected children. Physical manifestations and complications included stunting, wasting, lip changes and gross physical deformities. The bone age was delayed by 2 (range 1–6) years. There was peripheral muscle wasting and progressive generalised wasting. Four children had nodding as the only seizure type; 18 in addition had myoclonic, absence and/or generalised tonic–clonic seizures developing 1–3 years after the onset of illness. Psychiatric manifestations included wandering, aggression, depression and disordered perception. Cognitive assessment in three children demonstrated profound impairment. The EEG was abnormal in all, suggesting symptomatic generalised epilepsy in the majority. There were different degrees of cortical and cerebellar atrophy on brain MRI, but no hippocampal changes. Five stages with worsening physical, EEG and brain imaging features were identified: a prodrome, the development of head nodding and cognitive decline, other seizure types, multiple complications and severe disability. Conclusions Nodding syndrome is a neurological disorder that may be characterised as probably symptomatic generalised epilepsy. Clinical manifestations and complications develop in stages which might be useful in defining treatment and rehabilitation. Studies of risk factors, pathogenesis, management and outcome are urgently needed.
Sink–Source Balance and Down-Regulation of Photosynthesis in Raphanus sativus: Effects of Grafting, N and CO2

To clarify whether excessive accumulation of total non-structural carbohydrate (TNC) causes down-regulation of photosynthesis in Raphanus sativus, we manipulated sink-source balance to alter TNC levels in source leaves and examined its effects on photosynthetic characteristics, whole-plant biomass allocation and anatomical characteristics of leaves and petioles. Comet and Leafy varieties with large and small hypocotyls were reciprocally grafted to change hypocotyl sink strength. They were grown at high or low nitrogen (N) availability and at elevated or ambient CO2. Maximum photosynthetic rate, which was highly correlated with Rubisco and leaf N contents, was hardly correlated with TNC across the grafting combinations and growth conditions. Biomass allocation to petioles and hypocotyls and accumulation of TNC in each organ were significantly higher at low N. TNC and structural carbohydrates such as cellulose and hemicellulose were higher and the proportion of intercellular air space in source leaves was lower at low N and elevated CO2. We conclude that excess TNC does not cause severe down-regulation of photosynthesis, and cell walls and petioles are also major carbohydrate sinks responding to changes in sink-source and carbon-nitrogen balances, which contribute to alleviating further accumulation of TNC to avoid its negative effects in source leaves.
Day and Night GSH and MDA Levels in Healthy Adults and Effects of Different Doses of Melatonin on These Parameters

The pineal secretory product melatonin (chemically, N-acetyl-5-methoxytryptamine) acts as an effective antioxidant and free-radical scavenger and plays an important role in several physiological functions such as sleep induction, immunomodulation, cardiovascular protection, thermoregulation, neuroprotection, tumor-suppression and oncostasis. Membrane lipid-peroxidation in terms of malondialdehyde (MDA) and intracellular glutathione (GSH) is considered to be a reliable marker of oxidative stress. The present work was undertaken to study the modulating effect of melatonin on MDA and GSH in human erythrocytes during day and night. Our observation shows the modulation of these two biomarkers by melatonin, and this may have important therapeutic implications. In vitro dose-dependent effect of melatonin also showed variation during day and night. We explain our observations on the basis of melatonin's antioxidative function and its effect on the fluidity of plasma membrane of red blood cells. Rhythmic modulation of MDA and GSH contents emphasized the role of melatonin as an antioxidant and its function against oxidative stress.
Inferior Field Loss Increases Rate of Falls in Older Adults with Glaucoma

Purpose. To examine the visual predictors of falls and injurious falls among older adults with glaucoma. Methods. Prospective falls data were collected for 71 community-dwelling adults with primary open-angle glaucoma (mean age, 73.9 ± 5.7 years) for 1 year using monthly falls diaries. Baseline assessment of central visual function included high-contrast visual acuity and Pelli-Robson contrast sensitivity. Binocular integrated visual fields were derived from monocular Humphrey Field Analyzer plots. Rate ratios (RR) for falls and injurious falls with 95% confidence intervals (CIs) were based on negative binomial regression models. Results. During the 1-year follow-up, 31 (44%) participants experienced at least one fall and 22 (31%) experienced falls that resulted in an injury. Greater visual impairment was associated with increased falls rate, independent of age and gender. In a multivariate model, more extensive field loss in the inferior region was associated with higher rate of falls (RR, 1.57; 95% CI, 1.06 to 2.32) and falls with injury (RR, 1.80; 95% CI, 1.12 to 2.98), adjusted for all other vision measures and potential confounding factors. Visual acuity, contrast sensitivity, and superior field loss were not associated with the rate of falls; topical beta-blocker use was also not associated with increased falls risk. Conclusions. Falls are common among older adults with glaucoma and occur more frequently in those with greater visual impairment, particularly in the inferior field region. This finding highlights the importance of the inferior visual field region in falls risk and assists in identifying older adults with glaucoma at risk of future falls, for whom potential interventions should be targeted.
Performance Analysis of Decode-and-Forward Relay Network Under Adaptive M-QAM

In this paper, the performance of a cooperative Decode-and-Forward (DF) relay network with adaptive M-ary Quadrature Amplitude Modulation (M-QAM) is analyzed. A five mode adaptive M-QAM scheme is employed, and the system performance is investigated for both fixed switching levels and for optimized switching levels with a target bit error rate (BER) constraint. Expressions for average BER, spectral efficiency, and outage probability are derived to investigate the performance of the cooperative system under independently and identically distributed (i.i.d.) Nakagami-m fading wireless environments. Adaptive M-QAM with optimized switching levels maintains the target BER constraint while fixed switching levels achieves a conservative BER, which is lower than the target.
The Globular Cluster System of NGC 1399. II. Kinematics of a Large Sample of Globular Clusters

We study the kinematics and dynamics of the globular cluster system of NGC 1399, the brightest elliptical galaxy near the center of the Fornax cluster of galaxies. The observational data consists of medium-resolution spectra, obtained at the Very Large Telescope with FORS2 and the Mask Exchange Unit (MXU). Our sample comprises 468 radial velocities in the magnitude range 20 < mR < 23. This is the largest sample of globular cluster velocities around any galaxy obtained so far. Typical velocity uncertainties are 50 km s-1, significantly improving on earlier samples. The radial range is 2′ < r < 9′, corresponding to 11 kpc to 50 kpc of galactocentric distance. The shape of the velocity distribution of the sample is compatible with being a Gaussian distribution. However, under moderate error selection, a slight asymmetry is visible between high and low radial velocities. We find bright clusters with radial velocities below 800 km s-1, while they are not found at the corresponding high-velocity side above 2000 km s-1. There is the possibility that unbound clusters and/or objects in the foreground contaminate the NGC 1399 cluster sample. Under strong error selection, practically no objects are found with velocities lower than 800 km s-1 or higher than 2000 km s-1. Since the extreme velocities influence the velocity dispersion considerably, uncertainty regarding the exact value of the dispersion remains. With the above velocity limits, we derive a projected velocity dispersion for the total sample of 274 ± 9 km s-1 which within the uncertainties remains constant over the entire radial range. Without any velocity restriction, it increases to 325 km s-1. Guided by the bimodal color distribution of clusters, we distinguish between red clusters (C-R > 1.6) and blue clusters (C-R < 1.6), and find velocity dispersions for these groups of 255 ± 13 and 291 ± 14 km s-1, respectively, again radially constant. Any possible rotation of either of these cluster populations is below the detection limit, with the exception of a weak signature of rotation for the blue clusters more distant than 6′. Spherical models point to a circular velocity of 419 ± 30 km s-1, assuming isotropy for the red clusters. This value is constant out to 40 kpc. The inferred dark halo potential can be well represented by a logarithmic potential. A halo of the NFW type also provides a good fit to the observations. The orbital structure of the clusters can only be weakly constrained. It is consistent with isotropy for the red clusters and a slight tangential bias for the blue clusters. Some mass profiles derived from X-ray analyses do not agree with a constant circular velocity within our radial range, irrespective of its exact value. Interpreting the extreme low radial velocities as space velocities of bound clusters near their pericentric distances would require an extension of the cluster system of at least 200 kpc. Implications for formation scenarios of the cluster system are briefly commented on.
Optimizing cost and performance for multihoming

Multihoming is often used by large enterprises and stub ISPs to connect to the Internet. In this paper, we design a series of novel smart routing algorithms to optimize cost and performance for multihomed users. We evaluate our algorithms through both analysis and extensive simulations based on realistic charging models, traffic demands, performance data, and network topologies. Our results suggest that these algorithms are very effective in minimizing cost and at the same time improving performance. We further examine the equilibrium performance of smart routing in a global setting and show that a smart routing user can improve its performance without adversely affecting other users.
Example-based video remixing support system

Video remixes are generally created by sequentially arranging selected video clips and mixing them with other media streams such as audio clips and transition effects. Especially, mixing music clips often effectively improves the expressive quality of video clips. This paper proposes an example-based system for supporting average users in the 3 steps in video remixing: I)video shot sequence creation, II)music clip selection, and III)audio volume adjustment. The proposed system creates a template for a video remix and gives suggestions to users on an interface such as which video clips should be selected to create a video shot sequence and which music clips should be mixed to the created video shot sequence based on professionally created video remix examples. Then, the audio volume of each video shot is automatically adjusted based on its audio content so that the sounds in the video shots and the music clips would not interfere with each other. Experiments have verified that our system was able to create a video shot sequence whose quality was improved equally as the professionally created one by mixing the selected music clips, doubling the subjective scores from 1.7 to 3.7 on a scale of 1-5. Automatic audio volume adjustment improved the subjective scores by approximately 0.5 points on average. Further, the suggestions provided on the interface was evaluated useful by 5 subjects when creating a video remix by selecting 32 video clips and 4 music clips from 265 video clips and 180 music clips.
Motion processing across multiple topographic maps in the electrosensory system

Animals can efficiently process sensory stimuli whose attributes vary over orders of magnitude by devoting specific neural pathways to process specific features in parallel. Weakly electric fish offer an attractive model system as electrosensory pyramidal neurons responding to amplitude modulations of their self‐generated electric field are organized into three parallel maps of the body surface. While previous studies have shown that these fish use parallel pathways to process stationary stimuli, whether a similar strategy is used to process motion stimuli remains unknown to this day. We recorded from electrosensory pyramidal neurons in the weakly electric fish Apteronotus leptorhynchus across parallel maps of the body surface (centromedial, centrolateral, and lateral) in response to objects moving at velocities spanning the natural range. Contrary to previous observations made with stationary stimuli, we found that all cells responded in a similar fashion to moving objects. Indeed, all cells showed a stronger directionally nonselective response when the object moved at a larger velocity. In order to explain these results, we built a mathematical model incorporating the known antagonistic center–surround receptive field organization of these neurons. We found that this simple model could quantitatively account for our experimentally observed differences seen across E and I‐type cells across all three maps. Our results thus provide strong evidence against the hypothesis that weakly electric fish use parallel neural pathways to process motion stimuli and we discuss their implications for sensory processing in general.
Teacher reports of DSM-IV ADHD, ODD, and CD symptoms in schoolchildren.

OBJECTIVE To examine the prevalence of DSM-IV symptoms of attention-deficit/hyperactivity disorder (ADHD), oppositional defiant disorder, and conduct disorder and age, gender, and comorbidity differences between ADHD subtypes. METHOD Teachers completed a DSM-IV-referenced symptom inventory for 3,006 schoolchildren aged between 3 and 18 years. RESULTS The screening prevalence rate of ADHD behaviors was 15.8%; rates for individual subtypes were 9.9% for inattentive, 2.4% for hyperactive-impulsive, and 3.6% for combined. The inattentive type was relatively uncommon in preschool children (3.9%), whereas the hyperactive-impulsive type was least common in teenagers (0.8%). Screening prevalence rates were higher for African-American (39.5%) than white (14.2%) students, but did not vary significantly (p < .05) as a function of geographic region or socioeconomic status. ADHD subtypes were rated as more impaired than the non-ADHD group on most measures and were easily differentiated on the basis of comorbid symptoms, social skills impairment, and special education services. CONCLUSIONS The findings of this and similar studies show relatively high convergence for the prevalence of ADHD behaviors and differences between ADHD subtypes.
Ten years of shipboard ADCP measurements along the Northwestern Hawaiian Islands

Ten years of shipboard acoustic Doppler current profiler data, resulting in 105 transects along the Hawaiian Ridge, have been analyzed to describe the spatial and temporal variability of the mean currents and vertical shear structure in the vicinity of the Northwestern Hawaiian Islands. The analysis spans the period October 1 0 through November 000, with data being most sparse during the boreal winter months. The current field is dominated by mesoscale variability; only in a few locations is the mean statistically significant. The mean shows the North Hawaiian Ridge Current flowing westward south of Kauai and Nihoa. The average from March to July shows the eastward Subtropical Countercurrent, from Maro Reef to Necker Island. Information on ocean current structure is critical to better understand biological connectivity among the Northwestern Hawaiian Islands as well as between the Main Hawaiian Islands and the Northwestern Hawaiian Islands.
The Vulcanization of the Human Brain: A Neural Perspective on Interactions Between Cognition and Emotion

Emotions may explain inconsistencies in human behavior and forms of behavior that some have deemed irrational, though such behavior may seem more sensible after a discussion of the functions that emotions serve—or may have once served in our evolutionary past. People do have the capacity to override emotional responses. This capacity relies in large measure on the most recently evolved parts of our brains that support forms of behavior that are more recognizably rational. Neuroscientists are beginning to make headway in identifying the neural mechanisms involved in both emotional responses and higher cognitive processes. Among the most recent and exciting developments in neuroscience has been the introduction of methods for imaging the function of the intact human brain. This effort offers the promise of a deeper understanding of how and why emotions impact decision making, how this may contribute to behavior that appears to deviate from optimality, and how and when we are able to overcome such emotional responses.
Impact of Residual Additive Transceiver Hardware Impairments on Rayleigh-Product MIMO Channels With Linear Receivers: Exact and Asymptotic Analyses

Despite the importance of Rayleigh-product multiple-input multiple-output channels and their experimental validations, there is no work investigating their performance in the presence of residual additive transceiver hardware impairments, which arise in practical scenarios. Hence, this paper focuses on the impact of these residual imperfections on the ergodic channel capacity for optimal receivers, and on the ergodic sum rates for linear minimum mean-squared-error (MMSE) receivers. Moreover, the low- and high-signal-to-noise ratio cornerstones are characterized for both types of receivers. Simple closed-form expressions are obtained that allow the extraction of interesting conclusions. For example, the minimum transmit energy per information bit for optimal and MMSE receivers is not subject to any additive impairments. In addition to the exact analysis, we also study the Rayleigh-product channels in the large system regime, and we elaborate on the behavior of the ergodic channel capacity with optimal receivers by varying the severity of the transceiver additive impairments.
The Effect of Bone Marrow Mononuclear Cells on Lung Regeneration and Apoptosis in a Simple Model of Pulmonary Emphysema

Background In severe chronic stages of emphysema the only treatment is lung transplantation. SO, an urgent need exists for the development of effective treatments. Stem cells therapy arises as a new therapeutic approach. Aim of the Work To investigate whether bone marrow mononuclar cells (BMMNCs) can promote lung regeneration and decrease apoptosis in lipopolysaccharide (LPS) induced pulmonary emphysema in C57Bl/6 mice. Material and Methods 14 weeks old female mice (C57Bl/6), weighing around 25 g were used in this study. The mice were divided into 4 groups (10 in each group): group A: mice received no treatment, group B: mice received intranasal instillation of LPS with no further treatment, group C: mice received intranasal instillation of LPS then given a dose of BMMNCs and evaluated 21 days later and group D: the mice that received intranasal instillation of LPS then given a dose of Dulbecco’s Modified Eagle’s Medium (DMEM) and evaluated 21 days later. Imaging analysis was done using imagej program. To measure apoptotic index, Anti–caspase 3 polyclonal antibody staining was done. Results Analysis of the mean of airspace equivalent diameters (D0) and its statistical distribution (D1) for the different groups allowed to observe that group treated with BMMNCs (group C) showed the significant improvement in D0 and D1 than the group received LPS only (group B). Analysis of apoptotic index showed significant difference between BMMNCs treated group (group C) and that received LPS only (group B). Conclusions BMMNCs effectively promote lung regeneration and reduction of apoptosis in pulmonary emphysema.
Optical Properties of Low Loss (70dB/km) Hypocycloid-Core Kagome Hollow Core Photonic Crystal Fiber for Rb and Cs Based Optical Applications

We report on hypocycloid core shaped hollow-core Kagome fiber with a record loss of 70 dB/km at a spectral range of around 750-850 nm, which is compatible with Cs and Rb based applications. The fiber exhibits a single mode operation in a core diameter as large as 40 μm, and very low sensitivity to bend. We show experimentally that the bending-loss is limited by coupling between the guiding core mode and the modes in the cladding holes.