Miracle-dz/UniFAR-FTUs huggingface dataset:
Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routes.

PURPOSE To determine the afferent pathways linking the anterior chamber (AC) of the eye to the secondary lymphoid organs. METHODS Single intracameral, subconjunctival, or intravenous injections and topical application on the conjunctiva of 3 muL (30 mug) of cascade-blue-labeled Dextran (CB-Dx) were performed in Lewis rats. In addition, bilateral intracameral injections (CB-Dx into the right AC and FITC-Dx into the left AC) or a combination of intracameral (CB-Dx) and intravenous (FITC-Dx) injections were performed. Distribution of antigen-positive cells and free antigen in frozen sections of lymphoid organs from animals killed at 24 hours after these various types of injection was analyzed by fluorescence and confocal microscopy. RESULTS After intracameral and subconjunctival injections, antigen reaches the ipsilateral lymph node of the head and neck predominantly via the conjunctival lymphatics. Intraocular antigen entering the venous circulation reaches the spleen and mesenteric lymph nodes, but also a small proportion enters the lymph nodes of the head and neck. After bilateral intracameral injections, individual cells bearing both fluorescent antigens were identified in lymphoid organs draining the eye. Similarly, double antigen-positive marginal zone macrophages were observed after simultaneous intracameral injection of CB-Dx and intravenous injection of FITC-Dx. CONCLUSIONS These data demonstrate that in the first 24 hours after injection of antigen into the anterior chamber of the eye, antigen reaches the lymphoid organs mainly in a soluble form via both the blood and lymph.
The effect of endotoxin and tumour necrosis factor on erythrocyte and leucocyte deformability in vitro

Summary Microcirculatory disorders are a common finding in sepsis. We have analysed the influence of two factors released in sepsis, endotoxin and tumour necrosis factor (TNF), on rheological properties of blood cells. The deformability of mixed cell suspensions, isolated erythrocytes, mononuclear cells, or polymorphonuclear leucocytes exposed to endotoxin and TNF in vitro was assessed by filtration through pores of different sizes. Mixed blood cell suspensions showed an increase in cell rigidity when incubated with 100 ng/ml endotoxin. The filtration resistance of isolated erythrocytes, mononuclear or polymorphonuclear leucocytes was not affected by endotoxin. Incubation with TNF in physiological concentrations increased the rigidity of mixed blood cells and of isolated polymorphonuclear leucocytes in a dose‐ and time‐dependent manner, while erythrocytes and mononuclear leucocytes remained unaffected. Polymorphonuclear cells showed decreased deformability associated with shape changes (polarized and non‐polar cells with surface protrusions and a shift of F‐actin into protrusions). The decrease in deformability was reversed by cytochalasin B or xanthin derivatives such as pentoxifylline. We conclude that TNF decreases the passive deformability of polymorphonuclear leucocytes, which may affect the microcirculation in sepsis. The reversibility with xanthin derivatives may represent a new therapeutic approach for the high morbidity and mortality in sepsis.
22q11 deletion syndrome: a review of the neuropsychiatric features and their neurobiological basis

The 22q11.2 deletion syndrome (22q11DS) is caused by an autosomal dominant microdeletion of chromosome 22 at the long arm (q) 11.2 band. The 22q11DS is among the most clinically variable syndromes, with more than 180 features related with the deletion, and is associated with an increased risk of psychiatric disorders, accounting for up to 1%–2% of schizophrenia cases. In recent years, several genes located on chromosome 22q11 have been linked to schizophrenia, including those encoding catechol-O-methyltransferase and proline dehydrogenase, and the interaction between these and other candidate genes in the deleted region is an important area of research. It has been suggested that haploinsufficiency of some genes within the 22q11.2 region may contribute to the characteristic psychiatric phenotype and cognitive functioning of schizophrenia. Moreover, an extensive literature on neuroimaging shows reductions of the volumes of both gray and white matter, and these findings suggest that this reduction may be predictive of increased risk of prodromal psychotic symptoms in 22q11DS patients. Experimental and standardized cognitive assessments alongside neuroimaging may be important to identify one or more endophenotypes of schizophrenia, as well as a predictive prodrome that can be preventively treated during childhood and adolescence. In this review, we summarize recent data about the 22q11DS, in particular those addressing the neuropsychiatric and cognitive phenotypes associated with the deletion, underlining the recent advances in the studies about the genetic architecture of the syndrome.
State feedback control of uncertain networked control systems with random time-delays

This paper investigates the stabilisation problem for a class of linear uncertain networked control systems (NCSs) with random communication time-delays. Both sensor-to-controller and controller-to-actuator random network-induced delays are considered. Markov processes are used to model these random network-induced delays. Based on the Lyapunov-Razumikhin method a mode-dependent state feedback controller is proposed to stabilize this class of systems. The existence of such controller is given in terms of the solvability of bilinear matrix inequalities (BMIs), which are to be solved by a newly proposed algorithm. A numerical example is used to illustrate the validity of the design methodology.
Adherence of oral microorganisms to guided tissue membranes: an in vitro study.

Microorganisms can adhere and colonize on an exposed guided tissue regeneration (GTR) membrane thus developing a nidus of infection. The purpose of this study was to compare early bacterial adhesion to three different GTR membranes. Expanded polytetrafluoroethylene, polyglactin 910, and collagen were used as the test membranes. In part I of this study 15 different oral microbes were used to compare their relative ability to adhere to the membranes. Six of the most strongly adherent bacteria (Actinomyces viscosus, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans, Fusobacterium nucleatum, and Selenomonas sputigena) were selected for part II of this study. The membranes were placed in tubes containing broth cultures containing identical concentrations (1 x 10(8) cells/ml) of these bacteria at 37 degrees C. Membranes were placed in tubes of media without bacteria as controls. At 4, 6, 12, and 24 hours, the bacterial cultures were decanted and the membranes in the tubes were agitated gently in reduced transfer fluid (RTF) 4 times to remove non-adherent bacteria. Each tube was then sonicated for 30 seconds in 10 ml RTF to detach adherent bacteria. The detached adherent bacteria were counted using a Petroff-Hausser chamber. Data were analyzed by using the SAS program. Analysis of variance was used to test for differences between multiple means. Results showed S. mutans had the strongest attachment to the collagen membrane at 4 and 6 hours. Selenomonas sputigena had the lowest adherence capability to all test membranes. Polyglactin 910 had significantly (P < 0.05) lower S. mutants adherence than either the ePTFE or the collagen membrane at 4 and 6 hours. (ABSTRACT TRUNCATED AT 250 WORDS)
TREATMENT PROCESS IN PRISON THERAPEUTIC COMMUNITIES: MOTIVATION, PARTICIPATION, AND OUTCOME

Although the largest effects of prison-based therapeutic community (TC) programs are associated with entry into aftercare, only a minority of prisoners volunteer for these aftercare programs. The study addresses the gap in our knowledge concerning these low rates of voluntary entry. A theoretical formulation of the TC process involving the effect of the interaction of clients' motivation and participation in the activities of the TC on entry into aftercare was tested on a sample of 110 volunteers in a prison-based TC for whom there were client and staff ratings of 3-month participation and 12-month follow-up data on relapse and recidivism. Path analyses support a model in which the interaction of motivation and 3-month participation ratings have a direct effect on the selection of aftercare, and aftercare has a direct effect on relapse and recidivism. The use of a combination of enhanced motivation and early program participation as a means of increasing the utilization and effectiveness of aftercare is discussed.
Metabolic stress is a barrier to Epstein–Barr virus-mediated B-cell immortalization

Significance Epstein–Barr virus (EBV) was the first human tumor virus discovered. Although nearly all adults are infected with EBV, very few go on to develop disease, for reasons that we are only beginning to understand. Infection with EBV induces a period of very rapid cell division, which requires an increased supply of metabolites, such as nucleotides, amino acids, and lipids. We found that EBV-infected cells that are unable to meet this increased metabolic demand are forced to stop proliferating and undergo a permanent growth arrest called senescence. Epstein–Barr virus (EBV) is an oncogenic herpesvirus that has been causally linked to the development of B-cell and epithelial malignancies. Early after infection, EBV induces a transient period of hyperproliferation that is suppressed by the activation of the DNA damage response and a G1/S-phase growth arrest. This growth arrest prevents long-term outgrowth of the majority of infected cells. We developed a method to isolate and characterize infected cells that arrest after this early burst of proliferation and integrated gene expression and metabolic profiling to gain a better understanding of the pathways that attenuate immortalization. We found that the arrested cells have a reduced level of mitochondrial respiration and a decrease in the expression of genes involved in the TCA cycle and oxidative phosphorylation. Indeed, the growth arrest in early infected cells could be rescued by supplementing the TCA cycle. Arrested cells were characterized by an increase in the expression of p53 pathway gene targets, including sestrins leading to activation of AMPK, a reduction in mTOR signaling, and, consequently, elevated autophagy that was important for cell survival. Autophagy was also critical to maintain early hyperproliferation during metabolic stress. Finally, in assessing the metabolic changes from early infection to long-term outgrowth, we found concomitant increases in glucose import and surface glucose transporter 1 (GLUT1) levels, leading to elevated glycolysis, oxidative phosphorylation, and suppression of basal autophagy. Our study demonstrates that oncogene-induced senescence triggered by a combination of metabolic and genotoxic stress acts as an intrinsic barrier to EBV-mediated transformation.
The relationship of forensic odontology with various dental specialties in the articles published in a National and an International Forensic Odontology Journal: A 5-year content analysis

Aims: The aim of this study is to assess the quantum of articles published by various dental specialties in a National and an International Forensic Odontology Journal from January 1, 2010, to December 31, 2014. Settings and Design: The present study is a 5-year retrospective content analysis study. Subjects and Methods: Data were collected from two forensic odontology journals (Journal of Forensic Odonto Stomatology [JOFS] and Journal of Forensic Dental Sciences [JFDS]) which are subscribed by institutional library. The article contents were scrutinized by one investigator and categorized into nine individual dental specialties based on the new working classification proposed for forensic odontology. Statistical Analysis Used: The quantum of articles published by various dental specialties and the various focus areas in each specialty were assessed using Chi-square test. Results: Among all the published articles, a maximum number of articles were related to the Department of Oral Medicine and Radiology (32.6%) in JFDS with Cheiloscopy (46.7%) being more focused area and to the Department of Prosthodontics (25.7%) in JFOS with Bite mark analysis (66.7%) being more focused area. Conclusions: There was a scarcity of information about the relationship of forensic odontology with various dental specialties in the articles published in JFDS and JFOS. The editorial board of journals should expand and elaborate their scope of journals to various focus areas of forensic odontology. This will encourage the researchers to explore in the different focus areas which are most neglected as of now.
Does tip-of-the-tongue for proper names discriminate amnestic mild cognitive impairment?

ABSTRACT Background: Difficulty in retrieving people's names is very common in the early stages of Alzheimer's disease and mild cognitive impairment. Such difficulty is often observed as the tip-of-the-tongue (TOT) phenomenon. The main aim of this study was to explore whether a famous people's naming task that elicited the TOT state can be used to discriminate between amnestic mild cognitive impairment (aMCI) patients and normal controls. Methods: Eighty-four patients with aMCI and 106 normal controls aged over 50 years performed a task involving naming 50 famous people shown in pictures. Univariate and multivariate regression analyses were used to study the relationships between aMCI and semantic and phonological measures in the TOT paradigm. Results: Univariate regression analyses revealed that all TOT measures significantly predicted aMCI. Multivariate analysis of all these measures correctly classified 70% of controls (specificity) and 71.6% of aMCI patients (sensitivity), with an AUC (area under curve ROC) value of 0.74, but only the phonological measure remained significant. This classification value was similar to that obtained with the Semantic verbal fluency test. Conclusions: TOTs for proper names may effectively discriminate aMCI patients from normal controls through measures that represent one of the naming processes affected, that is, phonological access.
Using ALOS/PALSAR and RADARSAT-2 to Map Land Cover and Seasonal Inundation in the Brazilian Pantanal

The Brazilian Pantanal is a large continuous tropical wetland with large biodiversity and many threatened habitats. The interplay between the distribution of vegetation, the hydrology, the climate and the geomorphology nourishes and sustains the large diversity of flora and fauna in this wetland, but it is poorly understood at the scale of the entire Pantanal. This study uses multi-temporal L-band ALOS/PALSAR and C-band RADARSAT-2 data to map ecosystems and create spatial-temporal maps of flood dynamics in the Brazilian Pantanal. First, an understanding of the backscattering characteristics of floodable and non-floodable habitats was developed. Second, a Level 1 object-based image analysis (OBIA) classification defining Forest, Savanna, Grasslands/Agriculture, Aquatic Vegetation and Open Water cover types was achieved with accuracy results of 81%. A Level 2 classification separating Flooded from Non-Flooded regions for five temporal periods over one year was also accomplished, showing the interannual variability among sub-regions in the Pantanal. Cross-sensor, multi-temporal SAR data was found to be useful in mapping both land cover and flood patterns in wetland areas. The generated maps will be a valuable asset for defining habitats required to conserve the Pantanal biodiversity and to mitigate the impacts of human development in the region.
A Blood-Based Algorithm for the Detection of Alzheimer’s Disease

Background: We previously created a serum-based algorithm that yielded excellent diagnostic accuracy in Alzheimer’s disease. The current project was designed to refine that algorithm by reducing the number of serum proteins and by including clinical labs. The link between the biomarker risk score and neuropsychological performance was also examined. Methods: Serum-protein multiplex biomarker data from 197 patients diagnosed with Alzheimer’s disease and 203 cognitively normal controls from the Texas Alzheimer’s Research Consortium were analyzed. The 30 markers identified as the most important from our initial analyses and clinical labs were utilized to create the algorithm. Results: The 30-protein risk score yielded a sensitivity, specificity, and AUC of 0.88, 0.82, and 0.91, respectively. When combined with demographic data and clinical labs, the algorithm yielded a sensitivity, specificity, and AUC of 0.89, 0.85, and 0.94, respectively. In linear regression models, the biomarker risk score was most strongly related to neuropsychological tests of language and memory. Conclusions: Our previously published diagnostic algorithm can be restricted to only 30 serum proteins and still retain excellent diagnostic accuracy. Additionally, the revised biomarker risk score is significantly related to neuropsychological test performance.
Do edge responses cascade up or down a multi-trophic food web?

Despite nearly 100 years of edge studies, there has been little effort to document how edge responses 'cascade' to impact multi-trophic food webs. We examined changes within two, four-tiered food webs located on opposite sides of a habitat edge. Based on a 'bottom-up' resource-based model, we predicted plant resources would decline near edges, causing similar declines in specialist herbivores and their associated predators, while a generalist predator was predicted to increase due to complementary resource use. As predicted, we found declines in both specialist herbivores and predators near edges, but, contrary to expectations, this was not driven by gradients in plant resources. Instead, the increase in generalist predators near edges offers one alternative explanation for the observed declines. Furthermore, our results suggest how recent advances in food web theory could improve resource-based edge models, and vice versa.
Exact reconstruction with directional wavelets on the sphere

A new formalism is derived for the analysis and exact reconstruction of band-limited signals on the sphere with directional wavelets. It represents an evolution of a previously developed wavelet formalism developed by Antoine & Vandergheynst and Wiaux et al. The translations of the wavelets at any point on the sphere and their proper rotations are still defined through the continuous three-dimensional rotations. The dilations of the wavelets are directly defined in harmonic space through a new kernel dilation, which is a modification of an existing harmonic dilation. A family of factorized steerable functions with compact harmonic support which are suitable for this kernel dilation are first identified. A scale-discretized wavelet formalism is then derived, relying on this dilation. The discrete nature of the analysis scales allows the exact reconstruction of band-limited signals. A corresponding exact multi-resolution algorithm is finally described and an implementation is tested. The formalism is of interest notably for the denoising or the deconvolution of signals on the sphere with a sparse expansion in wavelets. In astrophysics, it finds a particular application for the identification of localized directional features in the cosmic microwave background data, such as the imprint of topological defects, in particular, cosmic strings, and for their reconstruction after separation from the other signal components.
Anti-HIV-1 Activity of Elafin Is More Potent than Its Precursor's, Trappin-2, in Genital Epithelial Cells

ABSTRACT Cervicovaginal lavage fluid (CVL) is a natural source of anti-HIV-1 factors; however, molecular characterization of the anti-HIV-1 activity of CVL remains elusive. In this study, we confirmed that CVLs from HIV-1-resistant (HIV-R) compared to HIV-1-susceptible (HIV-S) commercial sex workers (CSWs) contain significantly larger amounts of serine antiprotease trappin-2 (Tr) and its processed form, elafin (E). We assessed anti-HIV-1 activity of CVLs of CSWs and recombinant E and Tr on genital epithelial cells (ECs) that possess (TZM-bl) or lack (HEC-1A) canonical HIV-1 receptors. Our results showed that immunodepletion of 30% of Tr/E from CVL accounted for up to 60% of total anti-HIV-1 activity of CVL. Knockdown of endogenous Tr/E in HEC-1A cells resulted in significantly increased shedding of infectious R5 and X4 HIV-1. Pretreatment of R5, but not X4 HIV-1, with either Tr or E led to inhibition of HIV-1 infection of TZM-bl cells. Interestingly, when either HIV-1 or cells lacking canonical HIV-1 receptors were pretreated with Tr or E, HIV-1 attachment and transcytosis were significantly reduced, and decreased attachment was not associated with altered expression of syndecan-1 or CXCR4. Determination of 50% inhibitory concentrations (IC50) of Tr and E anti-HIV-1 activity indicated that E is ∼130 times more potent than its precursor, Tr, despite their equipotent antiprotease activities. This study provides the first experimental evidence that (i) Tr and E are among the principal anti-HIV-1 molecules of CVL; (ii) Tr and E affect cell attachment and transcytosis of HIV-1; (iii) E is more efficient than Tr regarding anti-HIV-1 activity; and (iv) the anti-HIV-1 effect of Tr and E is contextual.
Counseling Professionals' Attitudes toward Transgender People and Responses to Transgender Clients

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A Large-Droplet Mode and Prognostic Number Concentration of Cloud Droplets in the Colorado State University Regional Atmospheric Modeling System (RAMS). Part II: Sensitivity to a Colorado Winter Snowfall Event

Abstract This paper is the second in a two-part series describing recent additions to the microphysics module of the Regional Atmospheric Modeling System (RAMS) at Colorado State University. These changes include the addition of a large-cloud-droplet mode (40–80 μm in diameter) into the liquid-droplet spectrum and the parameterization of cloud-droplet nucleation through activation of cloud condensation nuclei (CCN) and giant CCN (GCCN). The large-droplet mode was introduced to represent more precisely the natural dual mode of the cloud-droplet distribution. The parameterized droplet nucleation replaces the former estimation of cloud-droplet formation solely from supersaturation calculations. In Part I of this series, details of the improvements to the microphysics were presented, including the set of equations governing the development of cloud droplets in the Lagrangian parcel model that was employed to parameterize this complex process. Supercell simulations were examined with respect to the model sensi.