Miracle-dz/UniFAR-FTUs huggingface dataset:
E1 Enzyme of the Pyruvate Dehydrogenase Complex in Corynebacterium glutamicum: Molecular Analysis of the Gene and Phylogenetic Aspects

ABSTRACT The E1p enzyme is an essential part of the pyruvate dehydrogenase complex (PDHC) and catalyzes the oxidative decarboxylation of pyruvate with concomitant acetylation of the E2p enzyme within the complex. We analyzed the Corynebacterium glutamicum aceE gene, encoding the E1p enzyme, and constructed and characterized an E1p-deficient mutant. Sequence analysis of the C. glutamicum aceE gene and adjacent regions revealed that aceE is not flanked by genes encoding other enzymes of the PDHC. Transcriptional analysis revealed that aceE from C. glutamicum is monocistronic and that its transcription is initiated 121 nucleotides upstream of the translational start site. Inactivation of the chromosomal aceE gene led to the inability to grow on glucose and to the absence of PDHC and E1p activities, indicating that only a single E1p enzyme is present in C. glutamicum and that the PDHC is essential for the growth of this organism on carbohydrate substrates. Surprisingly, the E1p enzyme of C. glutamicum showed up to 51% identity to homodimeric E1p proteins from gram-negative bacteria but no similarity to E1 α- or β-subunits of heterotetrameric E1p enzymes which are generally assumed to be typical for gram-positives. To investigate the distribution of E1p enzymes in bacteria, we compiled and analyzed the phylogeny of 46 homodimeric E1p proteins and of 58 α-subunits of heterotetrameric E1p proteins deposited in public databases. The results revealed that the distribution of homodimeric and heterotetrameric E1p subunits in bacteria is not in accordance with the rRNA-based phylogeny of bacteria and is more heterogeneous than previously assumed.
Disagreement, confusion, disapproval, turn elicitation and floor holding: Actions as accomplished by ellipsis marks-only turns and blank turns in quasisynchronous chats

This study evidences turn actions done by ellipsis marks-only turns and blank turns as employed in quasisynchronous chats that are not discussed in prior literature. A brief introduction to the research background of ellipsis marks in online chats is followed by a description of the data collected before delving into the actions done by ellipsis marks-only turns and blank turns. Data were culled from multi-party chats among tertiary students during a critical reasoning class. A Conversation Analysis-informed approach is applied in this article to analyze the preference organization of elliptical turns that illustrate several turn actions which include responses signaling disagreement, confusion and disapproval besides initial actions of eliciting responses and holding the floor. More than punctuation marks or paralinguistic restitution of silences, their interpersonal meaningfulness in sequential context and differentness/similarity vis-a-vis temporal silences are demonstratively shown in microscopic and .
Early-Life Hepatitis E Infection in Pigs: The Importance of Maternally-Derived Antibodies

Passive immunity (PI), acquired through colostrum intake, is essential for piglet protection against pathogens. Maternally-derived antibodies (MDAs) can decrease the transmission of pathogens between individuals by reducing shedding from infected animals and/or susceptibility of naïve animals. Only a limited number of studies, however, have been carried out to quantify the level of protection conferred by PI in terms of transmission. In the present study, an original modeling framework was designed to estimate parameters governing the transmission of infectious agents in the presence and absence of PI. This epidemiological model accounts for the distribution of PI duration and two different forces of infection depending on the serological status of animals after colostrum intake. A Bayesian approach (Metropolis-Hastings algorithm) was used for parameter estimation. The impact of PI on hepatitis E virus transmission in piglets was investigated using longitudinal serological data from six pig farms. A strong impact of PI was highlighted, the efficiency of transmission being on average 13 times lower in piglets with maternally-derived antibodies than in fully susceptible animals (range: 5–21). Median infection-free survival ages, based on herd-specific estimates, ranged between 8.7 and 13.8 weeks in all but one herd. Indeed, this herd exhibited a different profile with a relatively low prevalence of infected pigs (50% at slaughter age) despite the similar proportions of passively immune individuals after colostrum intake. These results suggest that the age at HEV infection is not strictly dependent upon the proportion of piglets with PI but is also linked to farm-specific husbandry (mingling of piglets after weaning) and hygiene practices. The original methodology developed here, using population-based longitudinal serological data, was able to demonstrate the relative impact of MDAs on the transmission of infectious agents.
Submodular Streaming in All its Glory: Tight Approximation, Minimum Memory and Low Adaptive Complexity

Streaming algorithms are generally judged by the quality of their solution, memory footprint, and computational complexity. In this paper, we study the problem of maximizing a monotone submodular function in the streaming setting with a cardinality constraint $k$. We first propose Sieve-Streaming++, which requires just one pass over the data, keeps only $O(k)$ elements and achieves the tight $(1/2)$-approximation guarantee. The best previously known streaming algorithms either achieve a suboptimal $(1/4)$-approximation with $\Theta(k)$ memory or the optimal $(1/2)$-approximation with $O(k\log k)$ memory. Next, we show that by buffering a small fraction of the stream and applying a careful filtering procedure, one can heavily reduce the number of adaptive computational rounds, thus substantially lowering the computational complexity of Sieve-Streaming++. We then generalize our results to the more challenging multi-source streaming setting. We show how one can achieve the tight $(1/2)$-approximation guarantee with $O(k)$ shared memory while minimizing not only the required rounds of computations but also the total number of communicated bits. Finally, we demonstrate the efficiency of our algorithms on real-world data summarization tasks for multi-source streams of tweets and of YouTube videos.
Eigenspace-based face recognition: a comparative study of different approaches

Eigenspace-based face recognition corresponds to one of the most successful methodologies for the computational recognition of faces in digital images. Starting with the Eigenface-Algorithm, different eigenspace-based approaches for the recognition of faces have been proposed. They differ mostly in the kind of projection method used (standard, differential, or kernel eigenspace), in the projection algorithm employed, in the use of simple or differential images before/after projection, and in the similarity matching criterion or classification method employed. The aim of this paper is to present an independent comparative study among some of the main eigenspace-based approaches. We believe that carrying out independent studies is relevant, since comparisons are normally performed using the implementations of the research groups that have proposed each method, which does not consider completely equal working conditions for the algorithms. Very often, a contest between the abilities of the research groups rather than a comparison between methods is performed. This study considers theoretical aspects as well as simulations performed using the Yale Face Database, a database with few classes and several images per class, and FERET, a database with many classes and few images per class.
Factors Influencing the Intention to Donate Blood: The Application of the Theory of Planned Behavior

A question about current availability of the blood has lifted up the concerns on how to keep the world population survive in the future. This has created the necessity to realize the underlying causes rely behind the scenario. The purpose of this study is to investigate the significant application of the Theory of Planned Behavior (TPB) in determining an individual intention and the actual behavior to involve in blood donation specifically in Perlis, Malaysia population. The questionnaire is adapted from prior researches on the application of the TPB. The findings indicated that, the TPB is relevant to the prediction of the intention to donate blood. Besides, among the three factors, Perceived Behavioral Control (PBC) is proved to be the best predictor. As well, the presents of blood donation experience also is said to have influence on an individual’s intention since those who ever donated their blood in the past have more intention to donate blood in future than those who are not. The findings from this study are very critical since they can help the authority to improve the current blood donation program and formulate a new strategy to call for more blood donors in the future.
Additional chromosomal abnormalities in patients with a previously detected abnormal karyotype, mental retardation, and dysmorphic features

The detection of chromosomal abnormalities in patients with mental retardation (MR) and dysmorphic features increases with improvements of molecular cytogenetic methods. We report on six patients referred for detailed characterization of chromosomal abnormalities (four translocations, one inversion, one deletion) detected by conventional cytogenetics, in whom metaphase CGH revealed imbalances not involved in the initially detected rearrangements. The detected abnormalities were validated by real‐time PCR. Parents were investigated by CGH in four cases. The genomic screening revealed interstitial deletions of 2q33.2‐q34, 3p21, 4q12‐q13.1, 6q25, 13q22.2‐q31.1, and 14q12. The estimated minimum sizes of the deletions ranged from 2.65 to 9.27 Mb. The CGH assay did not reveal imbalances that colocalized with the breakpoints of the inversion or the translocations. The deletion of 6q included ESR1, in which polymorphisms are associated with variation of adult height. FOXG1B, known to be involved in cortical development, was located in the 14q deletion. The results illustrate that whole‐genome molecular cytogenetic analysis of phenotypically affected patients with abnormal conventional karyotypes may detect inapparent molecular cytogenetic abnormalities in patients with microscopic chromosomal abnormalities and that these data provide additional information of clinical importance. © 2006 Wiley‐Liss, Inc.
Different goals, different predictions: Accuracy and bias in financial planning for events and time periods

Personal spending predictions are sometimes optimistically biased because predictors focus on their current savings goals. The present studies explored the role of savings goals in prediction by comparing spending predictions for time periods and discrete events. Contemplating a concrete event may elicit specific goals that compete with a focus on savings goals. Consistent with this hypothesis, Studies 1 and 2 revealed that participants relied less on savings goals, and were less biased, when predicting event spending rather than weekly spending. Study 3 demonstrated the causal impact of focusing on goals that compete with savings goals: Participants induced to focus on competing goals predicted to spend more money next week, and relied less on savings goals to generate their predictions.
X‐ray fiber diffraction analysis shows dynamic changes in axial tubulin repeats in native microtubules depending on paclitaxel content, temperature and GTP‐hydrolysis

Microtubules are key components of the cytoskeleton in eukaryotic cells. The dynamics between assembled microtubules and free tubulin dimers in the cytoplasm is closely related to the active shape changes of microtubule networks. One of the most fundamental questions is the association of microtubule dynamics with the molecular conformation of tubulin within microtubules. To address this issue, we applied a new technique for the rapid shear‐flow alignment of biological filaments, enabling us to acquire the structural periodicity data of microtubules by X‐ray fiber diffraction under various physiological conditions. We classified microtubules into three main groups on the basis of distinct axial tubulin periodicities and mean microtubule diameters that varied depending on GTP hydrolysis and the content of paclitaxel, a microtubule stabilizer. Paclitaxel induced rapid changes in tubulin axial repeats in a cooperative manner. This is the first demonstration of dynamic changes of axial tubulin repeats within native microtubules without fixation. We also found extraordinary features of negative thermal expansion of axial tubulin repeats in both paclitaxel‐stabilized and GMPCPP‐containing microtubules. Our results suggest that even in assembled microtubules, both GTP‐ and GDP‐tubulin dimers can undergo dynamic conversion between at least two different states: short and long configurations. © 2016 Wiley Periodicals, Inc.
Logical representations for automated reasoning about spatial relationships

This thesis investigates logical representations for describing and reasoning about spatial situations. Previously proposed theories of spatial regions are investigated in some detail - especially the 1st-order theory of Randell, Cui and Cohn (1992). The difficulty of achieving effective automated reasoning with these systems is observed. A new approach is presented, based on encoding spatial relations in formulae of 0-order ('propositional') logics. It is proved that entailment, which is valid according to the standard semantics for these logics, is also valid with respect to the spatial interpretation. Consequently, well-known mechanisms for propositional reasoning can be applied to spatial reasoning. Specific encodings of topological relations into both the modal logic S4 and the intuitionistic propositional calculus are given. The complexity of reasoning using the intuitionistic representation is examined and a procedure is presented with is shown to be of O(n3) complexity in the number of relations involved. In order to make this kind of representation sufficiently expressive the concepts of model constraint and entailment constraint are introduced. By means of this distinction a 0-order formula may be used either to assert or to deny that a certain spatial constraint holds of some situation. It is shown how the proof theory of a 0-order logical language can be extended by a simple meta-level generalisation to accommodate a representation involving these two types of formula. A number of other topics are dealt with: a decision procedure based on quantifier elimination is given for a large class of formulae within a 1st-order topological language; reasoning mechanisms based on the composition of spatial relations are studied; the non-topological property of convexity is examined both from the point of view of its 1st-order characterisation and its incorporation into a 0-order spatial logic. It is suggested that 0-order representations could be employed in a similar manner to encode other spatial concepts.
Statewide low-stakes tests and a teaching to the test effect? An analysis of teacher survey data from two German states

Governments worldwide are changing their educational policies to improve student performance. Some of these reforms involve the introduction of high-stakes testing, which often creates negative effects such as ‘teaching to the test’. This article deals with the use of low-stakes testing in the German states of Hesse and Bremen, and analyses whether statewide low-stakes testing produces comparable results to the use of high-stakes tests from the perspective of teachers. Moreover, it seeks to identify the factors responsible for the observable teaching to the test effect, above and beyond the use of statewide exams. The data come from surveys carried out in the framework of a longitudinal study funded by the German Research Foundation. Hesse and Bremen introduced statewide exit exams in several subjects in 2007 and 2008 as low-stakes tests. Our quasi-experimental study has been evaluating these implementations since 2007.
CD1d and CD1c Expression in Human B Cells Is Regulated by Activation and Retinoic Acid Receptor Signaling

B cell activation and Ab production in response to protein Ags requires presentation of peptides for recruitment of T cell help. We and others have recently demonstrated that B cells can also acquire innate help by presenting lipid Ags via CD1d to NKT cells. Given the newfound contribution of NKT cells to humoral immunity, we sought to identify the pathways that regulate CD1 molecule expression in human B cells. We show that ex vivo, activated and memory B cells expressed lower levels of CD1d compared with resting, naive, and marginal zone-like B cells. In vitro, CD1d was downregulated by all forms of B cell activation, leaving a narrow temporal window in which B cells could activate NKT cells. CD1c expression and function also decreased following activation by CD40L alone, whereas activation via the BCR significantly upregulated CD1c, particularly on marginal zone-like B cells. We found that the CD40L-induced downreglation of CD1d and CD1c correlated with diminished expression of retinoic acid receptor α (RARα) response genes, an effect that was reversed by RARα agonists. However, BCR-induced upregulation of CD1c was independent of the RAR pathway. Our findings that both CD1d and CD1c are upregulated by RARα signaling in human B cells is distinct from effects reported in dendritic cells, in which CD1c is inversely downregulated. One functional consequence of CD1d upregulation by retinoic acid was NKT cell cytotoxicity toward B cells. These results are central to our understanding of how CD1-restricted T cells may control humoral immunity.
Woman Abuse in Dating Relationships: An Exploratory Study

An exploratory survey of premarital woman abuse in a sample of Ontario male university students yielded an incidence rate of 70 percent. Consistent with many U.S. studies, "minor" forms of abuse were more frequently reported. Moreover, cohabitors were more violent than men involved in less serious dating relationships. Suggestions for further research are discussed.
Parent attitudes on the importance and success of integrated self‐contained services for students who are gifted, learning disabled, and gifted/learning disabled

This study reported on an assessment of parent attitudes of services provided by a specialized school for which the majority of the students were gifted, learning disabled (LD), or both. Parent perceptions were also surveyed on the services offered at their children's previous schools. In general, parents rated services of the specialized school as being significantly more important and successful as compared to the programs of the previous schools. The overall variance accounted for was 48.6% in the success ratings for the previous vs. specialized school. The higher the rated importance of the service in question, the higher was the perceived success. In general, the findings supported the need for specialized and integrated services for students who are gifted, LD, and gifted/LD, and the description of this particular specialized school provided a starting point for discussion on comprehensive and integrated programs to meet the needs of these students.
Cetacean Morbillivirus: Current Knowledge and Future Directions

We review the molecular and epidemiological characteristics of cetacean morbillivirus (CeMV) and the diagnosis and pathogenesis of associated disease, with six different strains detected in cetaceans worldwide. CeMV has caused epidemics with high mortality in odontocetes in Europe, the USA and Australia. It represents a distinct species within the Morbillivirus genus. Although most CeMV strains are phylogenetically closely related, recent data indicate that morbilliviruses recovered from Indo-Pacific bottlenose dolphins (Tursiops aduncus), from Western Australia, and a Guiana dolphin (Sotalia guianensis), from Brazil, are divergent. The signaling lymphocyte activation molecule (SLAM) cell receptor for CeMV has been characterized in cetaceans. It shares higher amino acid identity with the ruminant SLAM than with the receptors of carnivores or humans, reflecting the evolutionary history of these mammalian taxa. In Delphinidae, three amino acid substitutions may result in a higher affinity for the virus. Infection is diagnosed by histology, immunohistochemistry, virus isolation, RT-PCR, and serology. Classical CeMV-associated lesions include bronchointerstitial pneumonia, encephalitis, syncytia, and lymphoid depletion associated with immunosuppression. Cetaceans that survive the acute disease may develop fatal secondary infections and chronic encephalitis. Endemically infected, gregarious odontocetes probably serve as reservoirs and vectors. Transmission likely occurs through the inhalation of aerosolized virus but mother to fetus transmission was also reported.
Psychosocial Assistance for Youth: Toward Reconstruction for Peace in Angola

Following decades of war, Angolan youth are at risk of continuing cycles of violence and need support in developing positive behaviors and social roles. Accordingly, a community-based program, conducted in Angola 1998-2001, taught youth life skills, provided peer support and peace education, educated adults about youth, and engaged youth as workers on community development projects. The main results included increased adult awareness of the situation and needs of youth, improved youth-adult relations, reduced perceptions of youth as troublemakers, reduced fighting between youth, increased community planning, and increased perceptions that youth make a positive contribution to the community. The results suggest that a dual focus on youth and community development contributes to peacebuilding and the disruption of cycles of violence.
Expectant management of missed miscarriage

The option of expectant management was offered 221 women with ultrasound diagnosis of missed miscarriage. Eighty‐five women (38%) accepted; the remaining 136 women chose surgical evacuation of retained products of conception. In the expectant management group 21 women (24.7%) had a complete miscarriage, 14 (16.5%) had incomplete miscarriage necessitating surgery, and 50 (58.8%) requested surgery within 48 days from the original diagnosis. These results suggest that the success of expectant management of missed miscarriage is too low to justify its use in routine clinical practice.