definiens: means one or more of the following components : ( a ) a guide RNAelement, wherein said guide RNA element can be a guide RNA or a polynucleotide(s)encoding such guide RNA, that is complementary to a Target gene or safe harbor locus, and which is designed to be used with a naturally occurring or engineered clusteredregularly interspaced short palindromic repeats ( CRISPR)/CRISPR - associated ( Cas)protein, ( b ) a naturally occurring or engineered CRISPR - associated nuclease element, wherein said nuclease element can be a nuclease protein ( such as Cas protein ) or apolynucleotide(s ) encoding such protein, and which can be active or deactivated, and whichcan be complexed to an activation or repressor domain and ( c ) an HDR donor template, wherein said HDR donor template can be a single or double stranded polynucleotide(s),which is designed to be used with a nuclease and guide