definiens: means the process by which a fluorescent sample containing two distinct dyes wherein the sample is irradiated with light energy optimal to excite the first of the two dyes and wherein that first dye upon excitation transfers the energy to the second dye specifically when both dyes are attached to the same primer through a process whereby a nucleic acid template - dependent primer extension reaction determines the identity of a single nucleotide base at a specific position in a nucleic acid of interest, which process is covered by U.S. Patent No . 5,888,819 and 6,004,744 and any divisionals, continuation, reissues and foreign counterparts thereof, and the fluorescence emission of the second dye is detected at second wavelength, as and to the extent claimed in U.S. Patent No . 5,945,283 entitled Methods and Kits for Nucleic Acid Analysis Using Fluorescence Resonance Energy Transfer, issued on December 17, 1996 and owned or licensed by NEN Life Science Products, Inc., and any divisionals, continuations, reissues and foreign counterparts thereof and as and to the extent such NEN patents are valid, maintained and enforceable